LOCUS       DAVDWM010000001       103917 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVDWM010000001 DAVDWM010000000
VERSION     DAVDWM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 103917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.001"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            89..529
                     /locus_tag="VUW49_000001"
     CDS             89..529
                     /locus_tag="VUW49_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7979995.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            624..1053
                     /locus_tag="VUW49_000002"
                     /pseudo
     CDS             624..1053
                     /locus_tag="VUW49_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1124..1282
                     /locus_tag="VUW49_000003"
     CDS             1124..1282
                     /locus_tag="VUW49_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HET7979996.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1375..2178
                     /locus_tag="VUW49_000004"
                     /pseudo
     CDS             1375..2178
                     /locus_tag="VUW49_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            2195..2773
                     /locus_tag="VUW49_000005"
                     /pseudo
     CDS             2195..2773
                     /locus_tag="VUW49_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2786..2956
                     /locus_tag="VUW49_000006"
     CDS             2786..2956
                     /locus_tag="VUW49_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7979997.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(3462..4808)
                     /gene="gdhA"
                     /locus_tag="VUW49_000007"
     CDS             complement(3462..4808)
                     /gene="gdhA"
                     /locus_tag="VUW49_000007"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET7979998.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            5145..5896
                     /locus_tag="VUW49_000008"
                     /pseudo
     CDS             5145..5896
                     /locus_tag="VUW49_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5893..6111
                     /locus_tag="VUW49_000009"
     CDS             5893..6111
                     /locus_tag="VUW49_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7979999.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(6168..6452)
                     /locus_tag="VUW49_000010"
     CDS             complement(6168..6452)
                     /locus_tag="VUW49_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET7980000.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(6506..7441)
                     /locus_tag="VUW49_000011"
     CDS             complement(6506..7441)
                     /locus_tag="VUW49_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7980001.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            7545..7988
                     /locus_tag="VUW49_000012"
     CDS             7545..7988
                     /locus_tag="VUW49_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET7980002.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            8104..8370
                     /locus_tag="VUW49_000013"
     CDS             8104..8370
                     /locus_tag="VUW49_000013"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET7980003.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            8367..8741
                     /gene="crcB"
                     /locus_tag="VUW49_000014"
     CDS             8367..8741
                     /gene="crcB"
                     /locus_tag="VUW49_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7980004.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            8735..9064
                     /gene="crcB"
                     /locus_tag="VUW49_000015"
     CDS             8735..9064
                     /gene="crcB"
                     /locus_tag="VUW49_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7980005.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            9183..9530
                     /gene="rplS"
                     /locus_tag="VUW49_000016"
     CDS             9183..9530
                     /gene="rplS"
                     /locus_tag="VUW49_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7980006.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            9572..9643
                     /locus_tag="VUW49_000017"
     tRNA            9572..9643
                     /locus_tag="VUW49_000017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9605..9607,aa:Arg,seq:cct)
     gene            complement(9809..10807)
                     /locus_tag="VUW49_000018"
     CDS             complement(9809..10807)
                     /locus_tag="VUW49_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET7980007.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNDLECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(10850..11656)
                     /gene="yidA"
                     /locus_tag="VUW49_000019"
     CDS             complement(10850..11656)
                     /gene="yidA"
                     /locus_tag="VUW49_000019"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET7980008.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(11669..12973)
                     /locus_tag="VUW49_000020"
     CDS             complement(11669..12973)
                     /locus_tag="VUW49_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7980009.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            13045..13422
                     /locus_tag="VUW49_000021"
     CDS             13045..13422
                     /locus_tag="VUW49_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET7980010.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            13513..13845
                     /locus_tag="VUW49_000022"
     CDS             13513..13845
                     /locus_tag="VUW49_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET7980011.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            13857..15428
                     /gene="ffh"
                     /locus_tag="VUW49_000023"
     CDS             13857..15428
                     /gene="ffh"
                     /locus_tag="VUW49_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7980012.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15623..16906)
                     /locus_tag="VUW49_000024"
     CDS             complement(15623..16906)
                     /locus_tag="VUW49_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET7980013.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(17160..17873)
                     /gene="rsmG"
                     /locus_tag="VUW49_000025"
     CDS             complement(17160..17873)
                     /gene="rsmG"
                     /locus_tag="VUW49_000025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET7980014.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            17967..18527
                     /locus_tag="VUW49_000026"
     CDS             17967..18527
                     /locus_tag="VUW49_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7980015.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            18529..19428
                     /gene="htpX"
                     /locus_tag="VUW49_000027"
     CDS             18529..19428
                     /gene="htpX"
                     /locus_tag="VUW49_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET7980016.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19472..21031)
                     /locus_tag="VUW49_000028"
     CDS             complement(19472..21031)
                     /locus_tag="VUW49_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7980017.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRLLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(21270..21587)
                     /locus_tag="VUW49_000029"
     CDS             complement(21270..21587)
                     /locus_tag="VUW49_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET7980018.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYDHVKHVLSLYGPV
                     FEF"
     gene            21757..22299
                     /locus_tag="VUW49_000030"
     CDS             21757..22299
                     /locus_tag="VUW49_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET7980019.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            22299..22928
                     /gene="nth"
                     /locus_tag="VUW49_000031"
     CDS             22299..22928
                     /gene="nth"
                     /locus_tag="VUW49_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7980020.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            23139..23660
                     /gene="pyrR"
                     /locus_tag="VUW49_000032"
     CDS             23139..23660
                     /gene="pyrR"
                     /locus_tag="VUW49_000032"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET7980021.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            23679..24602
                     /locus_tag="VUW49_000033"
     CDS             23679..24602
                     /locus_tag="VUW49_000033"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7980022.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            24652..25731
                     /gene="carA"
                     /locus_tag="VUW49_000034"
     CDS             24652..25731
                     /gene="carA"
                     /locus_tag="VUW49_000034"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7980023.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            26044..29220
                     /gene="carB"
                     /locus_tag="VUW49_000035"
     CDS             26044..29220
                     /gene="carB"
                     /locus_tag="VUW49_000035"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7980024.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(29495..30304)
                     /locus_tag="VUW49_000036"
     CDS             complement(29495..30304)
                     /locus_tag="VUW49_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET7980025.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(30306..31109)
                     /locus_tag="VUW49_000037"
     CDS             complement(30306..31109)
                     /locus_tag="VUW49_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET7980026.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(31119..32606)
                     /gene="tacF"
                     /locus_tag="VUW49_000038"
     CDS             complement(31119..32606)
                     /gene="tacF"
                     /locus_tag="VUW49_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET7980027.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            32942..33649
                     /locus_tag="VUW49_000039"
     CDS             32942..33649
                     /locus_tag="VUW49_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7980028.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            33654..34676
                     /locus_tag="VUW49_000040"
     CDS             33654..34676
                     /locus_tag="VUW49_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7980029.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            34693..35562
                     /gene="licA"
                     /locus_tag="VUW49_000041"
     CDS             34693..35562
                     /gene="licA"
                     /locus_tag="VUW49_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET7980030.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            35552..36430
                     /locus_tag="VUW49_000042"
     CDS             35552..36430
                     /locus_tag="VUW49_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7980031.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            36442..37131
                     /locus_tag="VUW49_000043"
     CDS             36442..37131
                     /locus_tag="VUW49_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET7980032.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            37227..38075
                     /gene="dprA"
                     /locus_tag="VUW49_000044"
     CDS             37227..38075
                     /gene="dprA"
                     /locus_tag="VUW49_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET7980033.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            38965..40008
                     /locus_tag="VUW49_000045"
     CDS             38965..40008
                     /locus_tag="VUW49_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET7980034.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            40175..42262
                     /gene="topA"
                     /locus_tag="VUW49_000046"
     CDS             40175..42262
                     /gene="topA"
                     /locus_tag="VUW49_000046"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET7980035.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            42483..42842
                     /locus_tag="VUW49_000047"
     CDS             42483..42842
                     /locus_tag="VUW49_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET7980036.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            42935..43567
                     /locus_tag="VUW49_000048"
     CDS             42935..43567
                     /locus_tag="VUW49_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET7980037.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQAEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            43611..44267
                     /locus_tag="VUW49_000049"
     CDS             43611..44267
                     /locus_tag="VUW49_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET7980038.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            44286..45380
                     /locus_tag="VUW49_000050"
     CDS             44286..45380
                     /locus_tag="VUW49_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET7980039.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            45392..46429
                     /gene="leuB"
                     /locus_tag="VUW49_000051"
                     /pseudo
     CDS             45392..46429
                     /gene="leuB"
                     /locus_tag="VUW49_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            46426..46695
                     /locus_tag="VUW49_000052"
     CDS             46426..46695
                     /locus_tag="VUW49_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET7980040.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            46699..47058
                     /gene="leuD"
                     /locus_tag="VUW49_000053"
     CDS             46699..47058
                     /gene="leuD"
                     /locus_tag="VUW49_000053"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET7980041.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            47277..47420
                     /locus_tag="VUW49_000054"
     CDS             47277..47420
                     /locus_tag="VUW49_000054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980042.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            47681..47842
                     /locus_tag="VUW49_000055"
     CDS             47681..47842
                     /locus_tag="VUW49_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980043.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
     gene            48006..49937
                     /locus_tag="VUW49_000056"
     CDS             48006..49937
                     /locus_tag="VUW49_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET7980044.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            49924..51240
                     /locus_tag="VUW49_000057"
     CDS             49924..51240
                     /locus_tag="VUW49_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET7980045.1"
                     /translation="MQITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            51415..52401
                     /locus_tag="VUW49_000058"
     CDS             51415..52401
                     /locus_tag="VUW49_000058"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET7980046.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVSQLKADTPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            52465..52536
                     /locus_tag="VUW49_000059"
     tRNA            52465..52536
                     /locus_tag="VUW49_000059"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52498..52500,aa:Arg,seq:ccg)
     gene            52719..53417
                     /locus_tag="VUW49_000060"
     CDS             52719..53417
                     /locus_tag="VUW49_000060"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7980047.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            53408..56947
                     /gene="smc"
                     /locus_tag="VUW49_000061"
     CDS             53408..56947
                     /gene="smc"
                     /locus_tag="VUW49_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET7980048.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HVYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDQMARLSELLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELDRLSDGDWQADKEKCQDRLATIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            56944..57738
                     /locus_tag="VUW49_000062"
     CDS             56944..57738
                     /locus_tag="VUW49_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7980049.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            57738..58556
                     /locus_tag="VUW49_000063"
     CDS             57738..58556
                     /locus_tag="VUW49_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7980050.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKVAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            58560..59837
                     /gene="ftsY"
                     /locus_tag="VUW49_000064"
     CDS             58560..59837
                     /gene="ftsY"
                     /locus_tag="VUW49_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7980051.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(59883..61370)
                     /locus_tag="VUW49_000065"
     CDS             complement(59883..61370)
                     /locus_tag="VUW49_000065"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7980052.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(61519..62259)
                     /locus_tag="VUW49_000066"
     CDS             complement(61519..62259)
                     /locus_tag="VUW49_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7980053.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(62259..64424)
                     /locus_tag="VUW49_000067"
     CDS             complement(62259..64424)
                     /locus_tag="VUW49_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET7980054.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            64597..65550
                     /locus_tag="VUW49_000068"
     CDS             64597..65550
                     /locus_tag="VUW49_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980055.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            65627..67615
                     /gene="uvrB"
                     /locus_tag="VUW49_000069"
     CDS             65627..67615
                     /gene="uvrB"
                     /locus_tag="VUW49_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET7980056.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVVAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            67625..68188
                     /locus_tag="VUW49_000070"
     CDS             67625..68188
                     /locus_tag="VUW49_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980057.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            68154..68642
                     /locus_tag="VUW49_000071"
     CDS             68154..68642
                     /locus_tag="VUW49_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980058.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            68716..69183
                     /locus_tag="VUW49_000072"
     CDS             68716..69183
                     /locus_tag="VUW49_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET7980059.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(69180..69695)
                     /locus_tag="VUW49_000073"
     CDS             complement(69180..69695)
                     /locus_tag="VUW49_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET7980060.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(69707..70234)
                     /locus_tag="VUW49_000074"
     CDS             complement(69707..70234)
                     /locus_tag="VUW49_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7980061.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(70516..71079)
                     /locus_tag="VUW49_000075"
     CDS             complement(70516..71079)
                     /locus_tag="VUW49_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7980062.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(71063..71614)
                     /gene="coaC"
                     /locus_tag="VUW49_000076"
     CDS             complement(71063..71614)
                     /gene="coaC"
                     /locus_tag="VUW49_000076"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET7980063.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETLDEKTL"
     gene            complement(71626..72315)
                     /gene="coaB"
                     /locus_tag="VUW49_000077"
     CDS             complement(71626..72315)
                     /gene="coaB"
                     /locus_tag="VUW49_000077"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET7980064.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREVTNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            72545..74215
                     /locus_tag="VUW49_000078"
     CDS             72545..74215
                     /locus_tag="VUW49_000078"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET7980065.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            74543..75718
                     /gene="mutY"
                     /locus_tag="VUW49_000079"
     CDS             74543..75718
                     /gene="mutY"
                     /locus_tag="VUW49_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7980066.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            75774..76478
                     /locus_tag="VUW49_000080"
     CDS             75774..76478
                     /locus_tag="VUW49_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7980067.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            76471..77820
                     /gene="vicK"
                     /locus_tag="VUW49_000081"
     CDS             76471..77820
                     /gene="vicK"
                     /locus_tag="VUW49_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET7980068.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            77822..78631
                     /locus_tag="VUW49_000082"
     CDS             77822..78631
                     /locus_tag="VUW49_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7980069.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            78722..78964
                     /locus_tag="VUW49_000083"
     CDS             78722..78964
                     /locus_tag="VUW49_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET7980070.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            78966..79220
                     /locus_tag="VUW49_000084"
     CDS             78966..79220
                     /locus_tag="VUW49_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7980071.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            79267..80043
                     /locus_tag="VUW49_000085"
     CDS             79267..80043
                     /locus_tag="VUW49_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET7980072.1"
                     /translation="MNKKWLFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILIEKQSEKIEELHRMIKDLVEKIS"
     gene            complement(80302..81288)
                     /locus_tag="VUW49_000086"
     CDS             complement(80302..81288)
                     /locus_tag="VUW49_000086"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET7980073.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQVSAKELQAIIDEAWKNPEFQEASKN"
     gene            81482..83950
                     /gene="gyrA"
                     /locus_tag="VUW49_000087"
     CDS             81482..83950
                     /gene="gyrA"
                     /locus_tag="VUW49_000087"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7980074.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            83950..84693
                     /gene="srtA"
                     /locus_tag="VUW49_000088"
     CDS             83950..84693
                     /gene="srtA"
                     /locus_tag="VUW49_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET7980075.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            84937..85734
                     /locus_tag="VUW49_000089"
     CDS             84937..85734
                     /locus_tag="VUW49_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET7980076.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGVKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ANSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            85882..87141
                     /locus_tag="VUW49_000090"
                     /pseudo
     CDS             85882..87141
                     /locus_tag="VUW49_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            87237..88511
                     /locus_tag="VUW49_000091"
     CDS             87237..88511
                     /locus_tag="VUW49_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET7980077.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            88523..91708
                     /locus_tag="VUW49_000092"
     CDS             88523..91708
                     /locus_tag="VUW49_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980078.1"
                     /translation="MEDDLNYENLMDDVTEAIKKFNLVIFIGAGVSIAQGYPNWNNYI
                     EHLIKYWQGQVLSVSGEKRLGREHHVVFDLISKSSISNKRKVDLVNYELKKVFGEDFE
                     KRRLDFEKGYFKNLLPYSIVNQTVESLASLNAIFITSNYDYEIENHIKRLKNAVVTIN
                     DLNEFTKNKNGKLQFGDVLHIHGTPDCDVKYFVSSSADYSKTYLKNRENFENLVTWFK
                     ETKPTVLFVGAGLEEDEILSLLCKDSKNYALMKSENTGNQRVDEHYRGVVEGFFSSEN
                     HTQIIWYGDEFEKLPLFVKKLVADINEKLGTHDFYNQWNNLLNPSINQEEYNKNLDSI
                     SNDFKYLSSVLDKVIENDNNQLDQLMLNALLRSETLTVIKKNFVLAFWRFIVKNIEKL
                     SDNEWDVIYKIIYEGSQNYFIDDVFFVYNYAIDNKISSFTNNNKLNELREIISKDGYI
                     VNSNFNKDKTLLGYWLVSAFEQQNRDLYIKEDSEVEVNLNYECVNKLMSILNNPEFLS
                     YNYYSIEHQLKEYDVVKFLYELVKSKKLFIEEEKFLESDSEDLISTILIQKLLVQLDN
                     EINLDLEFIKRLIDKIDFSNIHFGEELNTFIKEHRSIIREKNIEIPKKPYRNWISSLE
                     GGFVSQFSYLTQENLVEYDESRVLEILVNAEKEQRGSSFLEEKTINETENFFITVLKE
                     SNEISKKVSDLLKNHIDDLYPKYKRLYVKIISFPEIEENLRKIVREKYLKRFNKESFD
                     SNDRKFFEYHIKQQNTDIDIFEKLLSINVNELSTPKGDNKQLDILHFINSEMGSYFQC
                     LISLFINHSSYRDVIIQIINSVTDTDYREFAQGILLNEYNPNRINVTYNTFLGFAYYH
                     STITIEAADVFTDVVRDILNKKIEDNQILNKVYLVALERVDPTIESFSLSKNNYSQMI
                     NIIFTGDYEFRYSKEWLGALFKFDSSANYLVTIFYLLYNENLKKNRFALFIEELSDYL
                     TTYNQKLSLRGMNYKLNHEELNNFDLLKKMFLKLMETDKIENDIFYLDGIKSILPLLS
                     LDDRRNVLQHIQKQNNCPPPEIEELQRIIVN"
     gene            91809..92687
                     /gene="truB"
                     /locus_tag="VUW49_000093"
     CDS             91809..92687
                     /gene="truB"
                     /locus_tag="VUW49_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7980079.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAKTPVLSSLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            92928..93230
                     /locus_tag="VUW49_000094"
     CDS             92928..93230
                     /locus_tag="VUW49_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET7980080.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            93338..93976
                     /gene="udk"
                     /locus_tag="VUW49_000095"
     CDS             93338..93976
                     /gene="udk"
                     /locus_tag="VUW49_000095"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET7980081.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            94104..95444
                     /locus_tag="VUW49_000096"
     CDS             94104..95444
                     /locus_tag="VUW49_000096"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7980082.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKVEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEVKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            95422..95634
                     /locus_tag="VUW49_000097"
     CDS             95422..95634
                     /locus_tag="VUW49_000097"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7980083.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            95631..96506
                     /locus_tag="VUW49_000098"
     CDS             95631..96506
                     /locus_tag="VUW49_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7980084.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            96499..97314
                     /locus_tag="VUW49_000099"
     CDS             96499..97314
                     /locus_tag="VUW49_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET7980085.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            97307..97738
                     /locus_tag="VUW49_000100"
     CDS             97307..97738
                     /locus_tag="VUW49_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET7980086.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            97745..99412
                     /gene="recN"
                     /locus_tag="VUW49_000101"
     CDS             97745..99412
                     /gene="recN"
                     /locus_tag="VUW49_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7980087.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYC
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            99414..100142
                     /locus_tag="VUW49_000102"
     CDS             99414..100142
                     /locus_tag="VUW49_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET7980088.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            100221..102044
                     /gene="lepA"
                     /locus_tag="VUW49_000103"
     CDS             100221..102044
                     /gene="lepA"
                     /locus_tag="VUW49_000103"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET7980089.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(102203..102283)
                     /locus_tag="VUW49_000104"
                     /pseudo
     CDS             complement(102203..102283)
                     /locus_tag="VUW49_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            102458..102934
                     /locus_tag="VUW49_000105"
     CDS             102458..102934
                     /locus_tag="VUW49_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7980090.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKGLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIDEAIA"
     gene            102972..103274
                     /locus_tag="VUW49_000106"
     CDS             102972..103274
                     /locus_tag="VUW49_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7980091.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAKQNMV"
     gene            103282..103917
                     /locus_tag="VUW49_000107"
                     /pseudo
     CDS             103282..103917
                     /locus_tag="VUW49_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        32009 a        18287 c        22725 g        30896 t
ORIGIN      
        1 ccaatattag tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg
       61 agtaaaagat aatcatctgg ataagcttgt gaaatctcct attcctaaag cccctttagc
      121 gcataacttt ggctcagctt ctattatcgc tcacaccatc catcagaagt ttaatctgaa
      181 ggtacccaat tatcgccaag aagaagattg ggctaggatg ggtttaccaa tcacacgtaa
      241 ggaaatctct aattggcata tcaaggcgag tcaatactat ttggagcccc tttataacct
      301 cttgcgagag agactattga ctcagccctt acttcatgcg gatgaaactt cttatagggt
      361 gctagagagt gatagtcagc tgacttacta ttggactttt ttgtcaggta aagcagagaa
      421 acaagggatt acgctttacc accatgatca gtgtcgaagt ggttcagtag tacaagaatt
      481 cctaggagat tattctggct atgtgcattg tgatattttg cggcagtaac ttaggacttt
      541 agtcctctag ttctgcctat gcgatagcag tccaaggttt aggagcaagg cgacgctaag
      601 cttggtaaac ttcgaaccgc tcgtctgctt atcgtcaact ggaagaagct gaacttgttg
      661 gatgttgggc gcatgtgaga aggaagtttt ttgaagcgcc cccccaagca agcggataaa
      721 tcatccttag gagctaaagg tttagcttat tgtgatcagt tattttcctt ggaaagagac
      781 tgggaggctt tgccagctga tgaacgacta cagaaacgtc aagaacatct ccagccctta
      841 atggaagact tctttgctta gtgccggcgt cagtcagttt tagcaggttc aaaactagga
      901 agggcaattg aatacagcct caagtatgaa gaaaccttta agaccatttt gaaagacgga
      961 catctggtcc tttccaataa tctagctgaa cgcgccatta aatcattggt tatgggacgg
     1021 agtaaaagag tccagtggac tcttttagcc taagctcagt ttaaaaaagc gagggtggtt
     1081 attttctcaa agttttgaag gagctaaagc aagagctatt attatgagtt tgttggaaac
     1141 agctaaacgt catcaattaa atagcgagaa atatctatcc tatcttctag aatgtcttcc
     1201 aaacgaggaa actctcgtaa acaaagaggt tttagaggct tatttaccat ggactaaagt
     1261 tgtacaagaa aagtgcaaat aagaaatctc cagattagga actatccgtg agttctccag
     1321 tctggagatt tttcaataga cttcctgcga aacaaaatat ggtataatag ttctatgaat
     1381 gatgaagcaa gtaaacaact aaccgatgca cgatttaagc gtcttgttgg tgttcaacgc
     1441 acgacttttg aagagatgtt agctgtatta aaaacagctt atcaacttaa acacgcaaaa
     1501 ggtggacgaa aacctaaatt aagtctagaa gaccttctta tggccactct tcaatatgtg
     1561 cgagaatatc gaacttatga acaaattgcg gctgtttttg gtattcacga aagcaactta
     1621 atccgtcgga gccaatgggt tgaagtaact cttgttcaaa gtggtgttac gatttcaaga
     1681 actcctctca gttctgagga cacggtaatg attgatgcga cggaagtaaa aatcaatcgc
     1741 cctaaaaaaa gaattagcga attattctgg taaaaagaaa tttcacgcta tgaaggctca
     1801 agcgattgtc acaagtcaag ggagaattgt ttctttggat atcactgtga actattgtca
     1861 tgatatgaag ttgttcaaaa tgagtcgcag aaatatcaga caagctggta aaatcttggc
     1921 tgacagtggt tatcaagggc tcatgaagat atatcctcaa gcacaaactt cacgtaaatc
     1981 cagcaaactc aaaccgctaa caattgaaga taaagtctat aaccatgcgc tatctaagga
     2041 gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatga tttcaacaac
     2101 ctatcgaaat catctaaacg cttcggatta cgaatgaatt tgattgctgg tattatcaat
     2161 catgaactag gattctagtt ttgcaggaag tctattatca aaaataccat caagattata
     2221 taagattgat acaggaaaag ttttatttga tggtgtaaat attaatcaaa tagataaaaa
     2281 aatattaagt caaaatttag gagtagttcc acaggattca tttttattga accgaagtat
     2341 tcttgataat ataactttaa agcacgaagt tacttcacaa aagatagagg aagtttgtaa
     2401 agcagttcaa atctatgatg aaatcatggc tatgccgatg aaatttaata ctatcatctc
     2461 agagatgggg tcaaatattt caggtgggca aaggcaacgg atagcactgg cacgtgcatt
     2521 aataaataat cctagtattg taattttaga tgaagcaact agtgcattag acactattaa
     2581 tgaggaaaga ataacaaagt atatacaaag tcagggctgt actcaaataa ttgtagctca
     2641 tagattgtca acgattaagg atgcggatgt tatttttgta atgaaaggtg gtaagattgt
     2701 tgagtcagga aatcataagt acttaatgga tcttggtgga gagtactaca gcttatatac
     2761 aaaaaggaaa tgaggtgtaa agaaaatgaa gaaagaaaat gaatatgtaa ttttaacaac
     2821 agcctcacta ggggtgatga ttggaatagt gtttgcaatt tttttagatt ttccagttga
     2881 atatggtatt tctttaggct tgttgaatgg aatagtattg ggttcgctga ttgtttacaa
     2941 aaacaataag aattaagcat aattttttgc tgtaaactaa ggagtagaga tggctatagt
     3001 tgaaattata aatctaacaa aaagctttaa agatattgaa gttattcata acacttaaat
     3061 aatagagcaa ctacagtagt agcttaaaaa catgattaaa tcgctattct taggagtagc
     3121 ggtttttctt tttgtttaat actctttgaa aatctcttca aaccacgtca gctttgcttt
     3181 accgtactca agtacagcct gcggctcgct tcctagtttg ctctttgatt ttcattgagt
     3241 ataaaaaggg tcaagtaagt atagtaaatt gaaataagat atgaacaaat cgattagaaa
     3301 agtcaaatta atttctagaa atatgttaga aattggtttg aattccgcaa tcaatttgtt
     3361 cagtttttat ttcatttcat tttatttaat tagattttcc aattttttaa ttcaagctaa
     3421 aaatccccaa tcgtagtgat tgaggattga gtaaataaat cttaaacaat accttgtgca
     3481 atcatggcat ttgctacatt ttcaaaggca gcaatgttag ctcctgcaag gtagtcttta
     3541 tcaagaccgt atgtttctga agtcgtttta gctgtgttga agatgtttgt catgatgtct
     3601 ttgagacggc catcaacttc ttcacgagtc catgagaggc gaagactgtt ttggctcatt
     3661 tcaagagctg aaacggctac accaccagcg ttggcagctt ttgcaggtcc gtagaagata
     3721 ccattttctt tgtaaacttt gatggcatca aggtcgctcg gcatgttggc accttcagat
     3781 acacagataa cgccttgagc aaccaaacgt ttagctgctt caccgttgat ttcgttttga
     3841 gtggcacatg gaagagcaat gtcatagttt ccagcgtaag tccatacagt accttcgtgg
     3901 taggttgcag ttgctttttc agctgcatac tcagtcaaac gagcacgacg tttttcttta
     3961 acatcaacca aaagatcgaa gtcgatacca ttttcatcga tgacataacc atttgagtca
     4021 gaaacagaaa taacagttgc accgagttca gttgcttttt gaagagcata ttgagcaacg
     4081 ttaccagaac ctgaaataac gactttctta ccagcaaagc tgttaccgtt agctttgagc
     4141 atttcttcag tatagtaaac caaaccgtaa ccagttgctt ctggacgaat caagctacca
     4201 ccaaatccaa gaggtttacc agtcaagaca ccagcatcaa attggttaag acgtttgtat
     4261 tgaccgtaaa ggtaaccaat ttcacgtcca ccaacaccga tatcaccagc aggtacgtca
     4321 agtgatggtc cgatgtgttt ttgcaattca gtcatgaagc tttggcagaa gcgcatcact
     4381 tcagcatctg ttttaccttt aggatcgaag tctgatccac ctttacctcc accgatagga
     4441 agtccagtca agacattttt aaagatttgt tcaaatccga ggaatttcaa gatcccttgg
     4501 tttacagttg ggtggaaacg aagtccacct ttgtatggtc caacagctga gttgaattga
     4561 acacggtaac cacggtttac ttgaattttt ccatcacggt caacccaagg aacacggaaa
     4621 gaaaccacgc gctcaggctc agtaatacgt gccaagatat tttcttcgat atactcaggg
     4681 tgtttttcaa atacaggttc taaagtgttg aaaaattctt caacagcttg gaggaattca
     4741 gcctcgtgcc cgttacgagc tttcacagtt tcaaacacgc tttggatata ttctttagca
     4801 gatgtcatat cgttctcctt ttattgtcat attttattac ataagacatt atagcagaat
     4861 tttttttcac tgtaaagaaa aaacagaaat tttctaaaaa ttctttcaat tcagtttttt
     4921 ggtttttttg aaaatagata aaaaaacgaa cgtttttctt ggaaatatct ttctcaaaga
     4981 caaaatctta aagtatgcta taagaaaaga aaatgtttag caattcagaa atcacttgtt
     5041 aatttatccg tgatatgtta gatttgccag taaaactatg accattttgg aggaaagcaa
     5101 tatttatacc agactaaaat caaaaagcat tatataatag taatatgaaa tcaactaaag
     5161 aagaaatcca aaccatcaaa acacttttaa aagactctcg tacagctaaa tattataaac
     5221 gccttcaaat cgttctatag taaaatgaaa ctagaacagg acaaatcgat ttctaacaat
     5281 gttttagaag tagaggtgta ctattctagt ttcaatctac tatatttcgt ctgatgggca
     5341 aatcttataa agagattata gagcttttag actgtaatca aacaacgatt tggcgaaacg
     5401 taaaaaaata tgaggagttc ggactcgact ctctccttca agaaacacgt ggtggtcgta
     5461 accatgcata tatgacagtt gaggaagaga aagcctttct tgcccgtcat ttgaaggcta
     5521 cagaggcagg agaatttgtt acaattgatg ccttatttca ggtttataaa aaggagtgtg
     5581 gatgaacgcc tttttagaag agctttcaca agcttatcct tttactcgtt atggacaatg
     5641 ctatatggca taaatcaagt accttaaata ttccgactaa tattgatttt acctttattc
     5701 ctccatacac accagagatg aacccattga acaagtgtgg aaagagattc gtaaacgtgg
     5761 atttaagaat aaagcctttc gaactttgga agatgtcatg aatcaacttc aagatgtcat
     5821 acaaggactg gagaaggagg tgataaagtc catcggtaat cggagatgga ctagaatgct
     5881 ttttgaaagc agatgagtat aagtcctcgt tttgaaacct tagagcaggc cattgcctcc
     5941 aaagatttgg aaaaagtccg agaagctttt aagaaaatga attccacttg gaccatcaat
     6001 gaaagtgtgg ttcgtgacaa tagtatagct cattatggac gtgttgaaac agctatttcc
     6061 ttcctgccta gtagcatgga aatcgaacct acagatgagt ctggaacatg aaaacagcag
     6121 ttgatttcaa gaaacaaaaa agaacagcca aaactgttct cttatcatta tttgagaccg
     6181 tattttttgt tgaaacgatc cacgcgtcca tctgcttgag tgaacttttg acgtccagtg
     6241 tagaatgggt gtgagtctga tgaaatttcc acacggatca atgggtaagt ttcgccttcg
     6301 aactcaactg tttcgttaga gcgttttgtt gaaccgctaa ggaattggta accagtagtt
     6361 gtgtccatga agacaactgg gcgatattct ggatggatat cttttttcat tttgcaatat
     6421 ttcctttctg ccatggtctc tttgcgagcc atagattgtt actatactag tctatcagat
     6481 tctgaaaagt ttggcaagta ttttatcagt ttttaagcaa gttttttaac ttttggtaga
     6541 tgatttcgtt ttcttctagg ctataggaat tagcaccact tgctagaggg tggcctccac
     6601 catcatgctc cttggcaatt tcattgatag gatggacttt actgcgtaag cgaactcggt
     6661 agtggccatc agcctgttcg acaaaaattc cccagagact cactctgtca atgcgtccag
     6721 gtgcacctac aatggccgca gtttcagcat cggttatatt gtattgtttc aagattttct
     6781 gactcaggat aacgcgagca gcaccatttt catccacttc cagatggtcg tagatgtagc
     6841 cttgcagttt agcaattttg tagctcatag tgtccatttt gcgagtgaga gccgcaaagt
     6901 caaagttatg ttctctcaaa taagcagcca ggcgaagagt ccgtgcagtg gtagaagggt
     6961 agaggaagcg acctgtatca ccgacaattc ctgcaaagag caactcagca tcgcgatctg
     7021 ccaaggctag ttgggttgtt tgggcaaata gggtaatcat ctcgctagcg ctacttgaac
     7081 tagtatcgac ccaagacagg tcaccgtata catcatcatt tggatggtgg tcaatcttaa
     7141 tgagaaaatc accttgacta tagcgcttat catcgatacg agcagtatta gctgtatcac
     7201 agacgatgac aagtgcgcct tggtaggctc tatcttcaac aagatccatc tcagccatcc
     7261 aagtaagagt tggttcatca aaaccgacgg ctttgatggt tttttctggg aaatgatgtt
     7321 ctagcaaggc tttcaatccc acctgacttc ccaaggcatc agggtctggt ttcatatgac
     7381 gatgaatgat aatggtgtcg tattctttaa ttttttctaa aatttgttgg caaatctcca
     7441 taaataacct caattctttc tcatttcatt gtagcacaag aatttttatt tttaaaatga
     7501 aaaagatgtg atataatgga gaaagataaa ttgaggagga cgatatggca ttagcaaaaa
     7561 ttgtatttgc cagtatgacc ggtaataccg aagaaattgc agatattgta gcagacaaat
     7621 tacgtgactt gggcttggat gttgatgttg atgaatgtac aactgttgac gcttcagact
     7681 tcttggaagc agacatcgct atcgttgcga cctatactta tggtgatggt gaattgccag
     7741 atgagatgat ggacttctac gaagacctag cagatctcaa cttgaatggc aaaatctacg
     7801 gagtggtcgg ctcaggagat accttctacg atgaattttg taaggctgtt gatgactttg
     7861 accgtgtctt tgtgtcaaca ggagcagaaa aaggttcaga gtgtgttaaa gttgatcttt
     7921 ctgccgagga agaagatatt gaacgcttgg aacaattcgc agaagaattg gctgctaaag
     7981 taggataagc ataaaagtgc gctgatgtaa tcaatcagtg catttttctt atcgagagtt
     8041 gggattttac tagcctaatt gatggctttt tgatacaata attcaaataa aggaggagac
     8101 tgtatggatt tagatattat tcgtcaagaa atcgatcaaa tcgacgacca aattgtcaaa
     8161 ctgttagaag aacgaatgca cttagttgaa ggagttgtcg cttataagaa ggcttcaggg
     8221 aaaccgattt tagatactaa gagagaagca gttatttttg aaaaggttag aagtcgtgta
     8281 gaggacaagc gctatcagga gactattgtc gcgacttttt cggacatact caaacgttcg
     8341 cgtgattatc aggatcaaaa tatcaaatga aaaaagaaca attttatccg ctagggattt
     8401 ttctagctgc tatgttgggc ggacttgtcc gctacctagt ttccacttgg ttaccagcca
     8461 gtccagattt cccttggggc actctctttg tcaactatct gggaattttc tgcttgattt
     8521 atcttgtcaa gggctatctg gtctataagg ggactagtaa gggcttgatt ttagcactgg
     8581 ggacgggttt ttgcggaggt ttaacaactt tttccagcct aatgcttgat actgtgaagc
     8641 tgcttgatac agggcgttat cttagtttga tactgtattt gcttttgagt atcggtggag
     8701 gcctgctttt agcttactat ttggggagga agaaatggta atcgtctatc ttgcaatcgc
     8761 ctgtggcttg ggagcactcg tacggtattt cttttctcgc tataatcaag cttctaaatt
     8821 gccactcgga acgctcatag ctaatctttt aggttgtttt ttaattggag tattctacaa
     8881 tcatgtagag tctaaggaag tatatgctat tctagcaaca ggattttgtg gcggtttaac
     8941 aactttttcg accttgaatg acgaacttca aagactgcta agtgataaga aggtctttta
     9001 ttcttacctg actttgactt acataggtgg tttggttgcg atttttttag gaattttgct
     9061 ataaatttct ttactttttt ttagaaattt ggtaaactat atcttgtgtg taatggacgc
     9121 acaaaaatac agtttatccg ctgaggaaga ttcctcaaga ttgacaaaga taggagaata
     9181 aaatgaatcc attaatccaa agcttgactg aaggtcaact tcgtacagat atcccatcat
     9241 tccgtcctgg tgacactgtt cgtgtacatg cgaaagttgt cgaaggtaac cgtgaacgta
     9301 tccagatttt tgaaggtgtt gttatcgcac gtaaaggtgc tggaatctca gaaaactaca
     9361 cagttcgtaa aatctctaac ggtgtaggtg ttgagcgtat cttcccaatc cacactccac
     9421 gtgttgaaaa aatcgaagtt gttcgttacg gtaaagtacg tcgtgcgaaa ttgtactact
     9481 tgcgtgctct tcaaggtaag gcagctcgta tcaaagaaat ccgtcgttaa ttcgactaaa
     9541 aaactctgct tttcagcaga gtttttatct agctccctta gttcaatgga tataacaact
     9601 ccctcctaag gagtagttgc aggttcgatt cctgcagggg gcagtaatta gatatcgaag
     9661 aagtttgtca aaaagtcttt gatattaaaa gaacgcataa tataggggat tgtctccata
     9721 atattatgcg ttttattatt gaaaaatttg ggcaatcatc agataaaatt gagactatat
     9781 cgagtatata aatgtttgta tagtcaattt attcaaaatc gtcaagttct aaattattta
     9841 aaggactata acattcatat ttctttaagt aattgtttat ttcttcagca gtggaacgag
     9901 gtaaattaac tcttaaatag tcaatatctt ccttagataa aaaatgattt gcattcaaaa
     9961 ttgagaagcg aaataatgct tcatcaaaag caaaatcata atcgtcatca gataatgatt
    10021 ccattaatgt ttgaatatgt tttagtgagt agtcaggtat cgaatcggga taatatactg
    10081 gataatcagt ggctgaaaaa ttgttttcta atcctgaaat agtaattatc aaagattcta
    10141 tgtaatatat taaagcatgt ttaatattac cttcttgttc caaatgtctt ccctgagcct
    10201 ttcgtgtgtt agataagcat ccaaaatctc ctgatacagt attcctatga gcttgtttat
    10261 ttaagagact ccattttata tcaccaaaag ttgctttaaa agagatattg tttttttctt
    10321 tacaataatc ttccatagtt atgtctttat catagtatgc tttgatatag tcactatgtt
    10381 cgataataag attttgtgct tcttttgtta gtttaaattc gaacggaata tctaaattgt
    10441 caatgttaac attattaatt attcttgtaa ttaattctgc tttttttcca gaagtagcta
    10501 aattatgttt agataatata gtttttaatt ctactactgt aagatatgat aaagtcttat
    10561 ttttagtgaa gtatcctaat ttgtataatt gattgtgtgt agattcaggg tcaatttgaa
    10621 gagagtattt aaaatatctt gggaaattat aatttctccc tgttttttga gtccaatcta
    10681 tgagtatgag ttgcccagga gtaaggccac attctaaatc gtttaggtaa aaattgttac
    10741 aatcagaatt tgtgtctcta ttatgattat tttgttttaa attaaatagt ttgctaaaaa
    10801 aattcattgg attttattgc taatcaattc ttattgaaaa atgatacttt tacagtaccc
    10861 atgttcggat ggcgtgggca acgccggatt cgtcatttgt tttagtgatg tatttggcga
    10921 tttttttgat ttctggattt ccattttcca tgacaacggg gtttccaacg acttccagca
    10981 tggcacggtc attttcttcg tcaccgattg ccatggtttc atctttggtc aatccgagtt
    11041 tttcagccaa gtgagtaata gctgaaccct tgtctacatt ctttttaagg agttcgaggt
    11101 agaaaggagc agatttgttg atagagtagt gctcgtaaaa ttcggctggt attttttcaa
    11161 tcgcagcatc gagaatttct ggttcatcga tgaacataca cttgacaatt tccttgccag
    11221 ccatttcttc aggggtacgg tagaagatag gcatgctgac gagggttgat tcgtgtacag
    11281 tgtattttcc gatattgcga tttgcagtat agataccgtc cttggtaatg gcatgcatgt
    11341 ggacaccgag cttgcgactg aggaattcca tatccagata atcctcatag gtcaaggatt
    11401 cgctgataat ctcatggcca gtagcagttt cttggacaag ggcaccattg aaggtcacca
    11461 catagtcacc ttggtctctc aactgcaagt cgtccagaag tttggcaaca cctgcgatgg
    11521 ggcggccagt tgcaatcacg actttgacac cagcttcttt ggcatcttgg atggcagaaa
    11581 agacttcagg agtgatttcc ttttggctgt tgactagggt tccgtcaata tcgacggcga
    11641 ttagtttaat actcataaat ttcctctttt agtttttatt tggggtaaaa tgatcgttct
    11701 caatcaagtg taaaaattgc tgggtgatgc ttgcgaagat gctgttttgg tctaacattt
    11761 ctttcgggaa ataaaagcga ttatctccgt ggcgacttcc agcaagggat tggacgatag
    11821 gggacaggct agagagttct gccagttctc catttttttg taaaatctca atctgtgtcc
    11881 ttggattttc agattcggga cggtaaatat cataagggag gtcaaagttc ttatgaatgg
    11941 cagtatagta gtcgggatca aagccgatgt cctcaaccaa ttttctcatg ctagcgagtt
    12001 ggtcttggtc ttcttgtgaa aaggtaatgg atttaaagac cttgcggttg acaaagcgat
    12061 gcgataaatc tgcaagaatc ttgtcaggac tggtcatcca aagctggaag taggtattca
    12121 tcacgccatc atccagagcc agatagtcag tcaaggtcac atttttttcg aagaaaggca
    12181 ggaggtgtgg agaagttcgg gcaaagaaat ccttgtcctc aggatagagt tccttggcgc
    12241 gtttgagaag attctgtagg agaacttcca tggcgcgtgt tgcggggtgg aaataaacct
    12301 gcatgtacat ctggtagcga ctgaggacgt agtcttcgat ggcgtgcatg ccattgcgct
    12361 gaaaggcgat accattttcg ataggacgaa tgactcggag gattcgagtc aggtcaaatt
    12421 ccccatagga tgctcctgta aaataggagt cgcgcaagag atagtccatg cgatctgcgt
    12481 caatctgact agaaatgagc tgcacgacct gcttattagg ataggtatgg tcaatgacac
    12541 tggccacctt ttctgggaaa tcaggtgcca cttgtagcag gacttggtga atctctgtct
    12601 caggattttg aataatctcc tgagtaatgg cttcatggtc tgtatcaaag agattttcaa
    12661 aagtatggga gtaggcacca tgcccaaggt cgtgtaggag agcagcggtc atggtcaaga
    12721 gagactcggc aggattccat tcctcaggat atttttcttc gaaaatctct gtgatgcgtc
    12781 gtgcaatttc atagactcct agacagtgag agaagcgact gtgttctcca ccgtggaagg
    12841 tataactgga agttcccagt tgtttgatcc ggcgcaaacg ctgaaattct tttgtattaa
    12901 tcaagtcata gatgatttga ttattgacat ggatgtagtt gtgaacaggg tcacggaata
    12961 ctttttcgtt catatgacca ttatagcaaa atcctgctct ttttggtaaa atagtaggac
    13021 aagagacaag aaagagggcc tgatgtgaag attcggatgc gaaatacaat tcagtttgat
    13081 gagcagttgg aagtaattga ccagctttat gatgtggaag tgcatgaaaa aggagattat
    13141 agctacctgc ttttctataa tgaggaaaag gaaaaagtag tgattaaatt tcatggtcaa
    13201 gaacttgtga tgagtcgatt ttccaatccc aagaccatta tgcgctttct aaaggatagt
    13261 gatagtttag cctatattcc cacacctatg ggtatgcagg agtttattat ccaaacgaat
    13321 cgttatcaag tggatgggca aaagattgaa ctagattatc aactacaaaa tcaagaggga
    13381 catccctttg ccagctatca attggaaatt acttggggct aggcttatgt ctttttcaaa
    13441 tttagctatc tatcttcttg gatcggcttc ctagtgtggt agattaggct aatttttggt
    13501 ataataaaag ttatggaaat cgaaaaaacc aatcgtatga atgcgctctt tgaattttat
    13561 gcggcgcttt tgacagataa gcaaatgaat tatatcgagc tctactacgc tgatgattac
    13621 agccttgctg aaattgccga ggagttcggt gtcagtcgtc aggctgtcta tgacaatatc
    13681 aagcgaacag aaaagattct ggaagattat gagatgaaat tgcacatgta ctcggactat
    13741 attgtccgca gtcagatttt tgatcagatt ttggagcgct atcccaagga taactttctg
    13801 caggagcaga tagaaatttt aacaagcatt gataatagag aataagagga agaaaaatgg
    13861 catttgaaag tttaacagaa cgtttgcaga acgtctttaa aagtctacgt aaaaaaggaa
    13921 aaatctctga atctgatgtc caagaggcaa ccaaagaaat tcgcttggcc ttgctcgagg
    13981 ccgacgttgc cttgcctgtt gtaaaggact ttatcaagaa agttcgtgag cgtgcagtcg
    14041 ggcatgaggt cattgataca cttaatcctg cgcaacagat tattaaaatc gttgatgagg
    14101 aactgacagc tgttttaggt tctgatacgg cagaaattat caagtcacct aagattccta
    14161 caatcatcat gatggttggt ttgcagggag ctggtaaaac aacttttgca ggtaagctgg
    14221 ctaacaaact caagaaggaa gaaaatgccc gtcctttgat gattgcggcg gatatttacc
    14281 gtcctgctgc cattgatcag ctgaagacac tgggacaaca gattgatgtg ccggttttcg
    14341 ctcttgggac agaggttcca gctgttgaga ttgtacgtca aggtttggag caagctcaaa
    14401 ctaatcataa cgactatgtc ttgattgata ctgcgggtcg tttgcagatt gatgagctcc
    14461 tcatgaatga gcttcgtgat gtgaaagcat tggctcaacc aaatgaaatc ttgcttgtcg
    14521 ttgatgctat gattggtcag gaagcagcca atgttgcgcg tgagtttaat gctcagttgg
    14581 aagtgactgg ggtcatcctt accaagattg atggcgatac tcgtggtggt gctgctctgt
    14641 ctgttcgtca cattactgga aaaccaatca agttcactgg tacaggtgaa aagattacgg
    14701 acattgaaac cttccaccca gaccgcatgt ctagccgtat ccttggcatg ggggatatgc
    14761 tcactttgat tgagaaagct tctcaggaat acgatgaaca aaaagccctt gaaatggctg
    14821 agaagatgcg cgaaaacacc tttgatttta atgatttcat cgatcaatta gatcaggtgc
    14881 aaaatatggg gccgatggaa gacttgctca agatgattcc aggtatggcc aacaatccag
    14941 cacttcaaaa catgaaggtg gatgaacgcc agattgctcg taaacgtgcg attgtgtctt
    15001 cgatgacacc tgaagaacgt gaaaatccag atttgttaaa tccaagccgt cgccgtcgta
    15061 ttgctgctgg ttctggaaat acattcgtcg aagtcaataa attcatcaag gactttaacc
    15121 aggctaaaca gctcatgcag ggtgttatgt ctggggatat gaataaaatg atgaagcaaa
    15181 tggggattaa tccaaataac cttcctaaaa atatgccaaa tatgggagga atggatatgt
    15241 ctgcccttga aggaatgatg ggacaaggcg gtatgcctga cttatcagct ctcggaggag
    15301 caggaatgcc agatatgagc cagatgtttg gtggcggttt gaaaggtaaa attggtgaat
    15361 ttgccatgaa acagtccatg aaacgtatgg ctaacaaaat gaagaaagcg aagaagaaac
    15421 gcaagtaaga caagagattg atgcaaatct catttgatga gaaaataaag taaaaatttc
    15481 tatgataaaa cgtacagtat caaggtttta gaatatctga tattatacgt tttttgattt
    15541 taataatttt gttaagacac ttttattata gtatacgaaa aatccactca gctgtctgag
    15601 tggattaggt gtatttagac tcttagtctt tattttcgta tggcaagatc aagttcaaga
    15661 tgattcctgt catggctgaa agggcagtac ctgaaagtgt aactggacca agtttaagga
    15721 tagctcctcc aagtccaaga accaacatag cacttgcgat gatgaggttt cgcatttgag
    15781 cgaaatcaac acgttcttta atcaagactt tcaaaccatt gctggcgata accccataga
    15841 gaaggattga cataccacca agtacagcgt ttggaatagt tgaaatcaag gcagtgaatt
    15901 taccaaggaa gctgagggca atcgcgatga aggcagcgtt acggataact gagacagaag
    15961 cgatacgagt cataccgata acccctgtat tttctccgta agttgtattg gctggtccac
    16021 caaggaaggc agaaacagaa gttgcgatac cgtcaccaat aagagtacgg tgaagacctg
    16081 gttcttttaa gaattgacgg ccacagattt gacccaaaac agtatggtct ccgatatgtt
    16141 cagaaattgt tacgatagcg attggcaaga tagcgatggc ttctggacca aagtaaagat
    16201 tgtactcttt aaaggcacca cctgtgctaa atggcaagta gaaaccagga atttcaaacc
    16261 agttagcttc taggacaggc gtaaagtcaa ccaaaccaag agttagcgcg aagaggtaac
    16321 caccgataat ggcaaagagg aatggaatga ttcgtaggaa gccttttcct tttgtattga
    16381 taaaggcagc aattaggaaa gtaacaacgg ctaccagagc atttttccaa tttccgtctg
    16441 ctacaagacc tgcattggta acagctgaac ctgcaagtcc aagaccgata acgatgatca
    16501 taggaccgat aatgattggt ggcaagagtt tatcaatcca ttttgttcct acaaatcgga
    16561 tgctggtagc aacaaggaca tagaccaaac cagtcaagat aacccctgtt tgagcagcag
    16621 atacatcccc ccccatttct ttcatagcca gtgacatagc tgtgataaag gcgaatgaag
    16681 aacctagata aactggaact ttaaaaccag tagcaatcat gtagatgagt gttccaacac
    16741 ctgaagcaaa aagggcaaca gatacaggca ttcccaaaat caatggtacc aagatggtcg
    16801 caccaaacat ggcgaaaacg tgttggaaac taaggagaat tccttttcca gccgaaggac
    16861 gttggtcaac gtctagtaac aaatcaacag ttgattcttg tttcatataa acctcacttt
    16921 tgggcatcaa aaaagagccc attattttac agcaataggc cctcagagta agacttcttt
    16981 aaaatttaga ttttaaagag ttttaaatat ttctgcgtct tcgtcacctc acgggatgac
    17041 attaaaaacc tttgcttagg atagtatagc agaaaaaaat gattttgtaa atcatttttt
    17101 cccgagccta gaaataaaag agcgaggctg attttgtaaa catttgttta ctaaaaaatt
    17161 taaagtgggc gtttatttgg cataccagcc ttacgtggat atttatttgg tgtttctttt
    17221 ttcttttcta ccactgtgat atagcgcgga tctctattcg gtagggcgta gctgagattg
    17281 tcttcgacct tactaaaaag gagattgagg gcattcttag cttctaataa ttcctcaggc
    17341 gcattgctag ccttgagtgc taatagtttg ccaccaacct taaggtaggg aatagtcaat
    17401 tcagatagga cctgcatacg ggcaaccgca cgagctgtta caaaatcata ttgagcacgg
    17461 aagttcttgt cttgggcaaa atcttcggca cgtccgtggt agaaatgaac tccgttcaaa
    17521 tccagttctt gagccaagag ttgtaggaag ttgatgcgct tattgagtga atcaataatg
    17581 gtcacatcta actccggata gagaattttc attggtagac taggaaatcc tgccccagcc
    17641 ccgatatcaa gaagtttgat agtttcattg ggaatcaaac cttgaagaat gggtgcaatc
    17701 gaatcgtaaa aatgtttgag ataaacttct tccttgtccg taatcgccgt caaattaatc
    17761 ttctcattcc actcgaccaa gagctcaaaa taacgttcaa attgttcttt ttgctggtcc
    17821 gaaagtggaa gattctgctc ggcaagcaag ttgtaaaatg tttttggttt catagttatt
    17881 tccttcttta gtatcatctt attttaccac aatcatagaa aatttgatat actggtacta
    17941 atctaaaagc agaaaggact tatattatga cttggattat tcttggagtt atcgctctta
    18001 ttgttatttt tgtgattgtt agctataacg gtttggttaa aaatcgtatg caaaccaagg
    18061 aggcttggag tcagattgat gttcagttga aacgtcgcaa tgacctcttg ccaaacttga
    18121 ttgagactgt aaaaggttat gccaaatatg aaggttctac ccttgaaaag gtggcagaac
    18181 tacgtaacca agtggcggca gcgacttcac cagcagaagc tatgaaagcc agtgatgccc
    18241 tcactcgtca ggtttcaggt atttttgcag ttgcagaaag ctatccagat ttgaaagcta
    18301 gtgctaactt tgttaaattg caagaggagt tgacaaacac agaaaataaa atttcttact
    18361 ctcgtcaact ctataacagt gttgtcagca actacaatgt aaaattagaa actttcccga
    18421 gcaatattat cgctggaatg tttggattta aagcggcaga tttccttcaa acacctgaag
    18481 aggaaaagtc ggttcctaaa gttgatttta gcggtttagg tgactaagat gttgtttgat
    18541 caaattgcaa gcaataaacg aaaaacctgg attttgttgc tggtattttt cctactctta
    18601 gctcttgttg gttatgcggt tggttatctc tttataagat ctggacttgg tggtttggtt
    18661 attgcactga ttatcggctt tatctacgct ttgtctatga tttttcaatc gacagagatt
    18721 gtcatgtcca tgaatggagc gcgtgaggtg gatgagcaaa cggcaccaga cctctaccat
    18781 gtagtggaag atatggctct ggtcgctcag attcctatgc cccgtgtttt catcattgat
    18841 gatccagcct taaatgcctt tgcgacaggt tctaatcctc aaaatgcggc tgttgctgcg
    18901 acttcaggtc tactagctat catgaatcgt gaagaactag aagctgttat gggacatgaa
    18961 gtcagtcata ttcgtaatta tgatatccgt atttcgacta ttgcagttgc ccttgctagt
    19021 gctatcacca tgctttctag tatggcaggt cgtatgatgt ggtggggtgg agcaggtcgc
    19081 agacgaagtg atgatgaccg agatggaaat ggtcttgaaa tcattatgct agtggtttcc
    19141 ctactagcta ttgtactggc acctctcgct gcaaccttgg ttcagctcgc tatttctcgt
    19201 cagagggaat ttctggcaga tgcatctagt gtcgagctga ctcgcaatcc ccagggaatg
    19261 attaatgccc tagataagtt ggacaatagc aaacctatga gtcgccacgt cgatgatgct
    19321 agcagtgccc tttatatcaa tgatcctaag aaaggtgggg ggttccaaaa actcttttat
    19381 acccacccac ctatctcaga acggattgaa cgtttaaaac agatgtaaaa gaatccagag
    19441 gtcgtctctg gatttttgct atgttgcaaa tttataaatc ttctaaatta actagcttca
    19501 aaccgtgttc tgtcatgcga tagatgatcg agcagacttc ttttaataaa cgacggtcat
    19561 gcgaaacagt gatgagaccg cctggatagg tagcaaagag ttttctgatt tggggttgag
    19621 aagtgggaga aaagtttcgt gtgggttcat ccagcaggag aaagtttggt ttgcgcagga
    19681 ctaaatccaa aagcaggagt tttccctgtt gtccgccaga taaggagcga atttgatgct
    19741 gcatttctgg ataactgaaa ttgagactag ctaggtgaga ttggattttc tgtagttcct
    19801 ctttttcccc agttttactg agataggcta ttggggataa atccaattgc agttttttgt
    19861 ggtaatcttg tggcataaaa ccaagtgaaa tctctctttt atcattcaga agtctctgta
    19921 acttggctaa cagagttgat ttcccaacac catttggccc gataataccg attttttctt
    19981 ggccacggac agttagttgt agtttttgaa ccaaaactcg gtcgtcaatg gacaaatttt
    20041 ctttttccag ttggactaag actttagaag ctggtaatgg ttggatgtct gaaaagaaaa
    20101 gttggatttg ttcctcttca agtggctttt gagtcatgga ctgagctgcc ttttcgtagc
    20161 gtttttcttg tgagaggaca gttttcatct ttttagccaa taggcgaccg gcagtactat
    20221 ctttggtagc tcgaagcgca gtttctacat tttgcttaac tctccgatgt ttttccatgg
    20281 ttttatcgta ggctctttgg ttgttagcag cttgctgact ttgtttggca aaattagcct
    20341 ttctctgctc actatagcta tcatagtcta aatgctctac tagcgtttcc gcttctttac
    20401 ggtgtttgac cagtcgcaag tgaacaatag tgtctgccgt ttcagaaaga aagtcttcat
    20461 catgggaaat gaaaataacg gtttgcctgg tcttttgaat ctggcctttt agccaatcaa
    20521 ctgtctcaag gtctaggtca tttgaaggtt catctaaaaa tagaatctca aagggtttgg
    20581 ctaactcatg gataagctga attttcaaag cttcgccccc tgatagattg ccaatctctt
    20641 ggtcacttgc gaaacgattg ctatcaaaat gcaattcctc cgccaaacga tagaggatac
    20701 tgtagtctaa atcaatagaa tctaaaaaga agtagtcgtg taaagttttc ttttttaggt
    20761 cctcagggac tttttgagga atgtaggcca gtgactgata gtcagattgg atgtttccct
    20821 tgatagtgaa atcagacaaa gcttccccca ttaaaatttt aagtaaggtt gatttgccat
    20881 ttccttcttc accaataata gcaacctttt ccccgtcttg gatggtcatg gttaggtcag
    20941 atacaagatc tcgtaaatct ttgttttgtg tgatggttag atgattgatt tttatcatat
    21001 gatttccctt tctagaatgt tcatagtgca taacaaaaag ctctacttta cctagtagag
    21061 cccaaagtct atgtcaaaaa aactctatta acatgccaga aaaagagcat aaaagagcac
    21121 acaaaaagag acaaaaacaa gatctactaa ataaaggttc tttatttgat agacttagtt
    21181 gttcatgtct cccttacctc cgagtattag tatctttata ttataccttt aaggaaattt
    21241 tgtcaacaga aaactggaaa ttctatgctc taaaattcaa aaacaggtcc atagagactt
    21301 agtacgtgtt tgacatgatc gtaatggaat ttatcatagt ttcgccaagc agtgcgtgct
    21361 tgatcgttta gttttaggct acccagtcca atcagaagac tggtaggttg gtaaaatttc
    21421 tcaagatcaa ttaagatact gttgttcaac tgttccgttt ttttgagctc ttgcatagta
    21481 gtgtttagca gctcttgtag tcgaatatca tctgccgtac cctgtttaca gttttggtag
    21541 cttttattta acttggatag gagttgaatg agtttcttgt cttccatgtg agcatcctcc
    21601 ttgatctgat gtactctttc cctatagtat agcactaaaa aataaaacta tcatagtaaa
    21661 tatgttaaaa ttttgaattt taattacaaa gtttggtgtg ctgatttgta gccttttcat
    21721 ggtataatca agagagtaaa tctttatgga ggaagtatga aattaaatat tcaagaaatt
    21781 cgtaagcagt ctgaaggttt gaactttgaa caaacgttag atttagttga tgacctgcgt
    21841 gcacgtaatc aagaaatttt agatgtaaaa gatatccttg cagttgggaa agtacaatat
    21901 gaagaccgta tgtatttctt agattatcaa ctatcttata ccattgttct tgcttcgagt
    21961 cgcagtatgg agccagttga gttagttgaa tcttatccag tcacggaagt tttcatggaa
    22021 ggcgcaacta accagctaga tcaagaagtt ttagatgatg acttggtctt gcccatcgaa
    22081 aatggggagc ttgaccttgc tgagagtgta tcagacaata tcctgctaaa cattcctatc
    22141 aaggtcttga cggctgaaga agaagctggt caaggattta tctcaggaaa tgactggcaa
    22201 atcatgacag aggaagaata ccaagctcaa aaagcagtaa agaaagaaga aaacagtcct
    22261 tttgctggct tacaaggact atttgacgga gatgaataat ggtcttgtca aaaaaacgag
    22321 cacgaaaggt gctagaagaa atcattgccc tcttcccaga tgccaagcct agtcttgatt
    22381 ttaccaatca ttttgaactc ctggttgcgg tcatgttatc agcccagacg acagatgcag
    22441 cggtaaataa ggccacacca ggtctctttg ttgcctttcc aacaccccaa gctatgtctg
    22501 tagcgacaga gagtgagatt gcttcacata tttctcgctt gggattgtat cggaataaag
    22561 ctaaattcct taaaaaatgt gcccaacagt tactagacga ttttgatggt caagtccctc
    22621 agacacgtga agaattggag agtttggcag gtgttggtcg caagacagcc aatgttgtca
    22681 tgagtgtagg atttggaatt ccagcctttg cagtggatac tcatgtggag cgtatttgca
    22741 aacaccacga tattgtcaaa aaatcagcga cgccacttga ggtggaaaag cgggtcatgg
    22801 atatcttgcc gcctgagcag tggttagctg cccatcaggc catgatttat tttggaagag
    22861 ccatttgcca tccaaaaaat ccagagtgtg accagtaccc acaattatat gattttagca
    22921 atttgtaaga tgctacattg aaactttttg cttgatttta agcatgcttt gtgctatagt
    22981 aaatgataac taaatattcc tttaaacctg tcccgtgagg caggcaagga gcgcgataaa
    23041 gtagaagggt gaagtctata ctgtgtgcag tagggctcgc ttggctataa atttcaagtc
    23101 tccttgttta aggagacttt tctttttgga ggtttgtcat gaaaacaaaa gaagttgtag
    23161 acgaattgac cgtcaaacga gcgattacgc gtattactta tgagattatc gaacgcaaca
    23221 aagatttgaa taagatcgtc ttggctggta ttaaaactcg tggtgtcttt attgcccacc
    23281 gaatccaaga acgtttgaag cagttagaaa atctttcagt tcctgttgtg gaattggata
    23341 ctaaaccttt ccgtgatgat gttaaaagtg gagaagatac ttctttggtt tctgtcgatg
    23401 tgacagaccg cgaagttatc ttggtggatg atgtgctcta tacaggtcgt accatccgtg
    23461 ctgctattga taatattgtc ggtcatggtc gtcctgcgcg tgtgagttta gcagttctag
    23521 tcgatcgtgg acatagagaa ttgccaatcc gtccagatta cgttggaaaa aatatcccaa
    23581 ccagtcgttc tgaagaaatc atcgtagaga tgacagaact tgatgaccaa gacagagttc
    23641 tgattactga agaagcttag aaagttaaag gagtagccat gtcagaaaat caacaagcat
    23701 tgaaccatgt ggtgtccatg gaagacctca ctgtcgatca agtaatgaaa ttgatcaagc
    23761 gaggaattga gtttaaaaat ggagcccagc ttccctatga agaccatccg attgtttcca
    23821 atctcttctt tgaggattct acacggacac ataagtcctt tgaagtcgca gagattaaac
    23881 ttggattgga acgacttgac tttgatgtga agactagctc ggttaataag ggtgagacac
    23941 tttatgacac cattttgact ctgtctgctt taggagtgga tgtctgtgtg attcgccatc
    24001 cagaggtcga ctactataga gaattgattg ctagtccaac gattacgact tccatcatca
    24061 atggtggaga tggttcgggc caacacccta gccagagctt gcttgatttg atgaccattt
    24121 atgaggaatt tggccacttt gagggtctta aagttgctat tgcaggtgac ttggaccact
    24181 cacgcgttgc caaatccaat atgcagattt tgaaacgctt gggagctgaa ctctttttcg
    24241 ctggacctga ggaatggaga agtcaagagt ttgcagacta tggacagttt gtaaccattg
    24301 atgaaatcat tgatcaggtt gatgtcatga tgtttctccg tgtgcaacac gaacgccatg
    24361 atagtggagc tgtattttca aaagaagact accatgccca acatggcttg actcaagaac
    24421 gttacgatcg tttgaaagaa acagcaatcc tcatgcaccc agctccaatc aatcgtgatg
    24481 tagaaatagc agaccacttg gttgaagcac caaaatcacg gattgtccaa caaatgacca
    24541 atggtgtctt tgttcgaatg gcaatcttag aatccgtact agcgagtaga aacgccaact
    24601 aaaacacaag acgttaaaag acgccagtga gcgtccacga gaggtgaaaa tatgacaaaa
    24661 agaattctag tattagaaga tggcacagtt tttgaaggca aggccttcgg agcagatatt
    24721 gatgtaacag gcgaaatcgt ctttaataca gggatgaccg gctaccaaga atccattaca
    24781 gaccagtctt ataatggaca aatcttgacc tttacttatc ctttggtagg aaattatggt
    24841 attaatcgtg atgattatga atccattatt ccaacttgta agggagtcgt tgtttttgaa
    24901 gaagcgcgta gagctagcaa ctggcgcaac caaatgacct tggatgaatt tttgaaagcc
    24961 aagaaaattc cagggatttc aggaattgat acgcgtgctc ttaccaagat tatccgtaag
    25021 catggtacta tgcgtgcaac cttgacccat gttggggaca gtatggacca tgtgacggac
    25081 cagctccaag caacagtttt accgacagac aatatcaaac aggtttctac taaaacgtct
    25141 tatccagctc caggagttgg tttgagcgtg gtgctagtgg actttggtct caagcactca
    25201 atcctacgtg aactttctaa acgcaactgt aacgtgacag ttgttcctta ttcgacaacg
    25261 gctgaagaaa ttctccatct caatcctgac ggagttatgt tgtcaaatgg tccaggtaac
    25321 ccagaagacg ttccacaagc actcgacatg attcgcggtg tacaaggaaa aattccaatc
    25381 tttggtattt gtatgggaca ccaactcttt gcaatggcaa acggggctaa gacctataag
    25441 atgaagtttg gccaccgtgg attcaaccat gcggtacgtg aaatcgcaac aggacgagta
    25501 gacttcacca gccagaacca tggttatgca gtcagccgcg aggatttgcc agagcacttg
    25561 attattaccc acgaagaaat caatgacaag tcagtggaag gtgtgcgcca cagataccaa
    25621 cctgctttct ctgttcaata ccacccagat gcagctcctg ggccacacga cgctagctac
    25681 ctatttgacg agtttatcga gatgatggaa gtttttaaac aatcaaacta agaacgagta
    25741 aaagctcgtc ggagaattta tgtaactgaa cacggacgga aagctcagaa tgtagagaaa
    25801 ccaacttgga ttgtcaatcc ttccttgatt tcctaaaatt caatcgcttt ctattcgtcc
    25861 tttgtatctt atttaatgtc gccctgagag gcgacgaata gaaaggagaa gggtgctccg
    25921 tatttgctga actgaatacg ggctacggac ggtgctaaaa agatagttat tcctagaact
    25981 gacagttctt cgtcataact cctattttag ctttgtccgc ttgacaccct taatatctta
    26041 tatatgccta aacgtactga tattcaaaaa attatggtga ttggttctgg tccgattatt
    26101 attggtcagg ctgctgagtt tgactacgct gggactcagg cttgcttgtc gttgaaagag
    26161 gaaggttatg aggttgtttt ggttaactca aatcctgcaa ccatcatgac ggacaaggaa
    26221 attgctgaca aggtttacat tgaaccgatt acacttgagt ttgtgacacg tattcttcgt
    26281 aaggaaggtc cagatgcctt gctaccaaca ctcggtggtc agacaggtct caacatggcc
    26341 atggaattat ctaaaaatgg tatcctagac gagcttggcg ttgaacttct gggtaccaaa
    26401 ttatctgcca ttgaccaagc ggaggaccgt gacctcttta aacaattgat ggaagagctt
    26461 gaacaaccca ttccagaatc tgaaattgtt aacacagtag aagaagcagt tgcctttgca
    26521 gcgacaattg gctacccagt catcgttcgt ccagccttta cccttggtgg tactggtggt
    26581 ggtatgtgtg ccaacgagaa agaattgcgt gaaatcactg aaaatggttt gaaattgtca
    26641 cctgttaccc aatgtttgat tgagcgttcg attgccggtt tcaaggaaat tgaatacgaa
    26701 gtgatgcgtg actcagctga caatgccttg gttgtttgta acatggaaaa ctttgaccca
    26761 gttgggattc acacagggga ttccattgta tttgcccctg cgcaaaccat gtcagactat
    26821 gaaaaccaaa tgcttcgtga tgcgagcttg agcattattc gtgccctcaa gattgaaggt
    26881 ggatgtaatg ttcagctagc tcttgatcct aacagcttca agtattatgt tatcgaagta
    26941 aaccctcgtg tatcacgttc gtcagccctt gcttctaaag cgacaggata tccgattgct
    27001 aagttggctg ccaagattgc agtaggtttg accttggatg aggttataaa cccagttaca
    27061 ggttcaacct atgccatgtt tgaaccagcc cttgactacg tagttgctaa gattccacgt
    27121 ttcccatttg acaagtttga aaagggtgaa cgccgtctcg gtacacaaat gaaggcgact
    27181 ggagaagtca tggcaatcgg tcgtaacatc gaggaatctc ttctcaaggc ctgccgctcc
    27241 cttgaaattg gggtgcacca caatgaaata cctgaacttg cagcagtttc tgatgatgcc
    27301 ttgattgaaa aggttgtcaa agcccaagat gaccgtctct tctacgtatc agaagccatt
    27361 cgtcgtggct acacacctga agaaattgca gagcttacaa aaattgatat cttctatctt
    27421 gataaactct tgcatatctt tgaaattgag caagaattgg gtgctcatcc acaagattta
    27481 gaagttttga aaacagccaa acttaatggt ttttcagacc gtaagattgc tgaactctgg
    27541 ggaacgactg atgataaagt tcgccaactt cgcttggaaa acaagattgt tccagtttat
    27601 aagatggtag acacttgtgc ggcagagttc gactccgaaa cgccatattt ctattcaacc
    27661 tatggttggg aaaatgaatc tatcagatct gataaagaat ctgtacttgt cctaggttca
    27721 ggtccaatcc gtatcggtca aggggttgag tttgactacg caaccgttca ctctgttaag
    27781 gctatccagg cagctggtta tgaagctatc atcatgaact caaacccaga gaccgtttca
    27841 acagacttct ctgtatcaga taagctttac tttgagccat tgacattcga agatgttatg
    27901 aacgtcattg acttggaaca accaaaggga gttatcgttc agttcggtgg tcaaacagcc
    27961 atcaaccttg cggagccatt ggcaaaagca ggtgtgacca tccttggtac acaggttgct
    28021 gacctagatc gtgccgaaga ccgtgacctc ttcgagcaag ctctcaaaga attggatatt
    28081 ccacagccac caggacaaac ggctaccaat gaagaagaag cagctcttgc agctcgcaag
    28141 attggcttcc cagttcttgt ccgcccatct tatgtacttg gtggacgtgc catggaaatc
    28201 gttgaaaacg aagaagacct tcgttcttac atgcgaactg ctgttaaggc tagtccagac
    28261 cacccagttc ttgtcgactc ttatatcgtt gggcaagagt gcgaagttga tgccatttca
    28321 gatggtaaaa atgtactcat tcctggtatc atggagcata tcgaacgtgc tggtgtccac
    28381 tcaggtgact caatggctgt ttacccacca caaaccttgt cgcaaaaggt tcaggagacc
    28441 atcgcagact acaccaaacg cttagcaatc ggtcttcact gccttggaat gatgaacatc
    28501 cagtttgtta tcaaggatga aaaagtctac gttattgagg tcaatccacg tgccagccgt
    28561 actgttccat tcctatctaa ggtaaccaat attcctatgg ctcaagtagc gaccaaactc
    28621 attcttggtc aaagtttatc agaacttggc taccaaaatg gactttaccc tgaaagcacc
    28681 cgtgttcata tcaaggcacc tgtcttctcc tttaccaaac tagctaaggt agacagcttg
    28741 ctcggtcctg aaatgaagtc aacaggtgaa gttatgggtt ctgatgctac tttggaaaaa
    28801 gctctctata aagcctttga agcttcttat ctacacttgc caacttttgg taatgtcgtc
    28861 tttaccatcg ctgatgatgc caaagaagaa gccttgaact tggctcgtcg tttccaaaac
    28921 attggttatg gaatccttgc gacagaaggg acagcagcct tctttgctag tcatggcttg
    28981 caagcccaac ctgttggtaa gattggtgat gacgataagg atatcccaag ttttgtacgc
    29041 aaaggaagaa ttcaagctat cattaacaca gtaggaacaa aacgaactgc tgacgaagat
    29101 ggtgagcaaa ttcgtcgttc agccattgaa cacggggtgc ccctcttcac agcgctagat
    29161 acagctaatg ccatgcttaa ggtacttgaa agtcgtagct ttgttacaga agcgatttaa
    29221 atagaaaaat aagatatagc agactgaatt tagaacagta cacaggaata ctaaaatatt
    29281 tctataaatt aatttgaatt ttctaattga tttgttcgca tcttatttca atctactata
    29341 gaaaaaagtc tttaaaatta taaaacgcat catatcaagg tttttcaaaa accttgatat
    29401 gatgcgtttt attgtgggaa tatgtatttc attttctact aaaattatgt ttttgaataa
    29461 cctctatctt agtagtttgt ataatccccc tcaatcagtt tttgcgataa gctttaatgc
    29521 tatgactata ccactcttgc atttcttttg atggtggtgt catataatcg ccatacatct
    29581 gggttaaaaa ttggtcatat tttttgggaa caggcaacat acggccctca aactcagtta
    29641 aaatcagttc tttaaaggta tcaactggga agatttcttt catcccttcc ttaccgatcc
    29701 caactcctcc ttcatattga ggagtgttgg ttacagcatt tttgactagt tgatcaattt
    29761 tcttgtaaaa gtagcgagga ttgacaaatc ggagagcgta ccagctacat aatctaagaa
    29821 aatcttttag tttgctatca ccgtgaactg ctcgtgattt tttgatataa gctagttgac
    29881 gaagagccac atacttatag ctcttgtcga caatgctcaa gtctgtaaat cgatcaattg
    29941 ggaagacatc gatgaaaagg ctggtatcat gacgcttgta cttaacatgg tcttctataa
    30001 cagtagaagt gtccaaaatc gatgcgaaat tatggaagta ccaagaagat gtatcgtagg
    30061 aaagaacctt gtagcgaggg tgattttctt cttcaataat cttcagtaaa cgctcataat
    30121 cctcacgata aagggaaata tcaatatcat catcccaagg aatcatacct ttgtggcgga
    30181 tggctccaag catggttcca taactgagaa aataaggaat atcatgtttc ttacaagtct
    30241 catcaatata gtccagcagg gctagttgaa tttctttaat ttcttttttt tctaaatatt
    30301 gcatcctaat cctccaattt ataagcgtga aattcatgac tgtagaagcg tttttcttct
    30361 ggtggtaggg tcatataatc tccataaaat tgtgtcaaaa tagtatcaaa tttttcaggt
    30421 gcaggaaggc ttaaattctc aaagggtaaa tcgattgttt tatcaaaggt accacttggg
    30481 aagacttcct tttccttaaa ttttgaaggg ataaaagcca tatattgccc attttcacga
    30541 ctatattttt gaatttcttt ctcgatttta tttgcaaaat aacgaggaga aaccggtcga
    30601 aggagtaacc agaaggctgt tcgtatccaa tcttttaaaa ggctatcctt atagacaata
    30661 tttttatgtt tactgaaaga cagcagtttg aagctttcca gtttataaca agtatcaatg
    30721 accttaggat catcaaagcg atctataggg aaaatatcga taaagatacc agactcatag
    30781 gtttttgtat ttcgagtatc aataatttta gtcgtactgt cggttatctt gataaagttg
    30841 ttaaagtagt tcttatcagt ttctaaggat aggagcttat acttgctttt ttccttttga
    30901 aaaatgttaa taaatcgttg gtagtcttct ctaggcatgg acagatcaat atcgtcgtcc
    30961 caagggataa agccctcatg tcgaaccgcc ccaatcagag taccgtagtt aataatatag
    31021 ttgatattgt gctttttaca gagagtatca atataatcca aaatttctaa ttcaatttgt
    31081 ttggcatctt caacggttag ttgtttcatt ttaaactcct atgatttttt aaatttattt
    31141 tttaaggcta ggacatggtt taaaaattca tagaaaatgc tatcttttgt gaagacaagt
    31201 agactaatat aagagatagc tgatagcaag acaatcaaac cagtattaat caaaaatggc
    31261 aaattaatga ccatatctac aggatacacg aaattaatca ggaaataaat tgctacaaag
    31321 gaaagtgaaa agagagagta tcgaacagta tagctaaaga tatgtcccaa gtggatgagt
    31381 tgttttctat ggatgaaaat gatatagaaa acaagtagag aagtctctga tagcatagtt
    31441 gtcagtaagt agtattcagg agccacgata tggttgaaaa aaaggagact attcaagccc
    31501 aaattgagta gcccagcaaa gactgtatag actgtgatac gtttttcata gccattggta
    31561 aagagaattt gggaaccaag aatggtatct aaggccagga taatcgtacg aaaagcgaag
    31621 agagaggtca agatgccgcc tccgatatat ttttcactac cgtaaagtag gatggcattt
    31681 ggtcctaaaa ccatgagtcc aaaactcagt ggaatgataa agaagttaaa gattcgacta
    31741 cctctattaa ccagagaaac ataggcttct ttgtctcctt tccccagata gtaactgaga
    31801 cgaggcacac tcactccaat tgcacctgtt acaaccccag ctataacggt cacaattcgc
    31861 tgagctatgg tatagtaact aacgttgaca tcaatccctg ttttaacgag gaagaggcga
    31921 tctaaaaaag tgaagagcat attggcattg gcaaagacta acatggctgt cagagggaga
    31981 aagagtggtt taaaatcact taggtgaatt ttaacaagtt tgatgtctct tttaatccaa
    32041 aaataactaa tcaggtagtt aatcagcgtc gataaactca tcacaagtgt atagacaaca
    32101 atatcgtgtt catttttaac aaataagaaa atagagacca gcatcaggat acggatgaag
    32161 gcagttttgt aaaagagaaa actgtaattt tccagagctt cattgaccca ttcgattgaa
    32221 aaaatctggg caatgagttg aatccccata acaaggtaga cctttttgac gattggatta
    32281 tcagtaaaga agagaggata ggctaggata tagacagcag tggtcaaaat cgtacaagcg
    32341 atgcacaaat aaaaaagact agaaaaggtt ctgttaagat cttttttgtt atccttgaca
    32401 ttactgatag cccttaaacc gtagttatag acaccataag ttgcaaaggg caagaaaaat
    32461 gacaaaatag tgtcgactga gttgaagtaa ccatagtcag ttcggtccaa gacacgcgcg
    32521 acataggttc cagttaggat gggaaaaata atattcaaga cacgaattcc catgtaagat
    32581 agagcattta attttatact tttcattcaa tttacctcgt ttttcattat atcataaagt
    32641 tagctaataa gaaatgaagg gcagtaagtc aagtaatcac tttgaagttt caaatcttaa
    32701 gttttaagtt ttctttaagg aaagtatatt attctgaagg actctaaaat ttcgcagcca
    32761 tttattagta attgctacag aattcctagt cattactaga aatggactag tttctttgaa
    32821 taatagaact gcataattct cctattctag aaggggagga ccagtatttc ttttatgata
    32881 ggactagatt gtggtataat agagagaata agttttttta gtaagacaaa ggagaaaata
    32941 gatgatttat gcaggaattc ttgccggtgg aactggcaca cgcatgggga tcagtaactt
    33001 gccaaaacaa tttttagagc taggtgatcg acctattttg attcatacaa ttgaaaaatt
    33061 tgtcttggag ccaagtattg aaaaaattgt agttggtgtt catggagact gggtttctca
    33121 tgcagaagat cttgtagata aatatcttcc tctttataag gaacgtatca tcattacaaa
    33181 gggtggtgct gaccgcaata caagtattaa gaacatcatt gaagccattg atgcttatcg
    33241 tccgcttact ccagaggata tcgttgttac ccacgattct gttcgtccat ttattacact
    33301 tcgcatgatt caggacaata tccaacttgc ccaaaatcat gacgcagtgg acacagtggt
    33361 agaagcggtt gatactatcg ttgaaagtac caatggtcaa tttattacag atattccaaa
    33421 tcgtgctcac ctttatcaag gacaaacacc tcaaacattc cgttgcaagg acttcatgga
    33481 cctttatgga tctctttctg atgaagagaa ggaaatcttg acagatgcat gtaaaatctt
    33541 tgtgatcaaa ggaaaagatg tggctttggc caaaggtgaa tactcaaatc tgaagattac
    33601 aaccgtaaca gatttgaaga ttgcaaaaag tatgattgag aaagactagt aaaatgatta
    33661 atcaaattta tcaactaact aagcctaagt ttatcaatgt caaatatcag gaagaggcta
    33721 ttgaccaaga gaatcatatc cttatccgtc ccaactacat ggctgtctgt catgcggatc
    33781 agcgttacta tcagggaaaa cgtgatccca agattttgaa taaaaagctt ccaatggcaa
    33841 tgattcacga gtcatgtgga accgtcattt ctgacccgac cggaacctac gaggttggtc
    33901 aaaaagttgt catgattccc aatcagtctc ctatgcagag tgatgaagaa ttctatgaaa
    33961 actacatgac agggacccat ttcttgtcta gtggatttga tggctttatg agagagtttg
    34021 tttctctccc taaagatcgt gtggtggctt atgatgctat tgaagatacg gttgcagcca
    34081 ttacagagtt tgtcagtgtg ggcatgcacg ctatgaatcg tctattgact cttgctcata
    34141 gcaagcggga gcggatcgcc gttattggag atggaagttt agcttttgtg gttgccaata
    34201 ttatcaacta tactttgcca gaagcagaga ttgtggttat tggtcgtcat tgggaaaagt
    34261 tggaactctt ctcatttgcc aaagaatgct atattacgga taatattcct gaagatttgg
    34321 cctttgacca tgcttttgaa tgttgtggtg gtgatggtac tggaccagct attaatgact
    34381 tgattcgcta cattcgtcct cagggaacga ttctcatgat gggagttagc gaatataaag
    34441 tcaatctcaa tactcgcgat gccttagaaa agggcttgat tttggttggg tcatctcgtt
    34501 ctggtcgcat tgattttgaa aatgctatcc aaatgatgga agtcaagaaa tttgccaatc
    34561 gtcttaaaaa tatcctttat ctagaagaac ctgtaagaga aattaaagat attcatcgtg
    34621 tctttgcaac cgatttaaac acagccttta aaacagtgtt taagtgggaa gtataagtac
    34681 tggaggttaa ttgtggagaa aatcattaaa gaaaaaattt cttccttact tagtcaagaa
    34741 gaggaagtcc tcagtgttga acaactgggt ggaatgacca atcaaaacta tttggccaaa
    34801 acaacaaata agcaatacat tgttaaattc tttggtaaag ggacagaaaa gcttatcaat
    34861 cgacaagatg aaaagtacaa tcttgaacta ctaaaggatt taggcttaga tgtaaaaaat
    34921 tatctttttg atattgaagc tggtatcaaa gtaaatgagt atatcgaatc tgcgattacg
    34981 cttgattcaa cgtcaatcaa gaccaagttc gacaaaatta ctccaatatt acaaactatt
    35041 catacgtctg ctaaggaatt aagaggagaa tttgctcctt ttgaagaaat caaaaaatac
    35101 gaatccttga ttgaagaaca aattccttat gccaactatg aatctgttag aaatgcagtc
    35161 ttctccttag agaaaagact ggctgactta ggtgttgaca gaaaatcttg tcatatcgat
    35221 ttggtgcctg aaaactttat cgaatcacct caaggacgac tttatttgat tgactgggaa
    35281 tattcatcaa tgaatgatcc aatgtgggat ttggctgccc tctttttaga gtctgaattc
    35341 acttcccaag aggaagaaac tttcttatct cactatgaga gtgaccaaac accggtttct
    35401 catgaaaaga ttgctattta taaaatttta caagatacta tttggagtct atggactgtc
    35461 tataaggaag agcaaggtga agattttggt gactatggtg tgaatcgtta ccaaagagct
    35521 attaaaggtt tggcttctta tggaggttca gatgaaaagt aaaaacggag ttccttttgg
    35581 ccttctctca ggtattttct ggggcttggg tctaacggtt agtgcttata tcttttcgat
    35641 ttttacagat ttgtcaccct ttgtggtggc tgcaactcat gattttttga gcatctttat
    35701 cttactagct tttctcttgg taaaagaagg gaaagttcgc ctctcaattt tcttaaatat
    35761 tcgcaatgtc agtgttatca tcggagcctt gctagcaggc cctatcggta tgcaggccaa
    35821 tctttatgca gttaagtata tcggaagttc tttagcttca tctgtatcgg ctatttaccc
    35881 tgcgatttca gttctattgg ctttcttctt tttgaagcac aagatttcga aaaatactgt
    35941 atttgggatt gtcttgatta ttggagggat tattgctcag acctataagg ttgaacaggt
    36001 taattctttc tacattggga ttctttgtgc tttggtttgt gctattgcat ggggaagtga
    36061 gagtgttctt agctcttttg ccatggaaag tgaattgagt gaaatcgaag ccctcttaat
    36121 ccgtcaagta acttcgttct tgtcctatct tgtgattgtg ctcttctctc atcagtcatt
    36181 tactgcagta gccaatggac aattgctagg tctcatgatt gtttttgcag cctttgatat
    36241 gatttcctac ttggcttatt atatcgctat caatcgcttg caaccagcca aggctacagg
    36301 cttgaacgtg agctatgtag tatggacggt cttgtttgca gttgttttct tgggtgcacc
    36361 gctagatatg ctgaccatta tgacgtcact tgtcgtcatt gctggagttt atattattat
    36421 taaagaataa aggagattcg tgtgaaagcc attatcttag cagcgggatt gggaactcgc
    36481 ttgcgtccta tgactgaaaa tacccctaaa gccttggttc aggttaatca aaaacctttg
    36541 attgagtacc aaattgagtt tctcaaagaa aaaggaatca atgacatcat catcattgtt
    36601 ggttatctta aagaacaatt cgattacttg aaagagaaat acggtgttcg tctcgttttc
    36661 aatgataaat acgctgacta caataacttt tactctctct atcttgtaaa agaagaattg
    36721 gccaacagct atgttattga tgctgacaat tatctcttta aaaatatgtt ccgcaatgat
    36781 ttgacacgtt cgacttattt tagtgtttat cgtgaagatt gtaccaacga atggttcttg
    36841 gtttatggag atgactacaa ggttcaagac attattgttg atagcaaggc aggtcgcatc
    36901 cttagtggtg tatccttctg ggatgctcca actgcagaaa agattgtcag ctttatcgac
    36961 aaggcttatg taagtggtga atttgttgat ctctattggg acaatatggt taaggataat
    37021 atcaaagagc tagatgtcta tgttgaagaa ttagaaggca atagcattta tgagatcgat
    37081 agtgtccaag actatcgtaa attagaagaa attcttaaaa acgaaaatta aagattccaa
    37141 catctgacaa aatagtcgga tgttttttga ttttttacga acttttacga atagatagat
    37201 gagtagaaaa agaaatggag ttatttatga aaatcacaaa ctatgaaatc tataagttaa
    37261 aaaaatcagg tttgaccaat caacagattt tgaaagtgct agaatacggt gaaaatgttg
    37321 atcaggagct tttgttgggt gatattgcag atatctcagg ttgccgtaat ccagccgttt
    37381 ttatggaacg ttattttcag atagacgatg cgcatttgtc gaaagagttt caaaaatttc
    37441 catctttctc tattttagat gactgttatc cttgggattt gagtgaaata tatgatgcgc
    37501 ctgtactttt attttacaag ggaaatcttg acctcctgaa attcccgaag gtagcggtcg
    37561 tgggcagtcg tgcttgtagc aaacagggag ctaagtcagt tgaaaaagtc attcaaggct
    37621 tggaaaatga actggttatt gtcagtggtc tggccaaggg cattgacaca gcagctcata
    37681 tggcagctct tcagaatggc ggaaaaacca ttgcagtgat tggaacagga ctggatgtgt
    37741 tttatcctaa agccaataaa cgcttgcaag actacatcgg caatgaccat ctggttctaa
    37801 gtgaatatgg acctggtgaa caacctctga aatttcattt tcctgcccgt aatcgcatca
    37861 ttgctggact ttgtcgtggt gtgattgtag cagaggctaa gatgcgttca ggtagtctca
    37921 ttacgtgtga gcgagcaatg gaagaaggac gcgatgtctt tgctattcct ggtagcattt
    37981 tagatggact atcagacggt tgccatcatt tgattcaaga aggagcaaaa ttggtcacca
    38041 gtgggcaaga tgttcttgcg gaatttgaat tttaaaaatg acctaagcta gaattctaag
    38101 aaaaaatcaa ttttaagaga aaatgaaccc aacatttcca taataaaacg catattagca
    38161 agtttttaac acttgataat atgcgttttt tctaagtgga ttagtagagt agaggatttt
    38221 tctcatataa tactcttcga aaatctcttc aaactacgtc agcttccatc tgcaacctca
    38281 aaacagtatt ttgagctgac ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag
    38341 caacctgtgg ctagcttcct agtttgcgct ttgattttca ttgagtataa gggaaagtat
    38401 agtgaattga aataagatgt gaataactct atcaggaaag tcaaattaat ttatagaaat
    38461 attttagcag ccaaggtgta ctgttataga ttcaattaca ctataattta gtgtaattga
    38521 gaaaggagaa atgattgtga ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct
    38581 caaatcttgt cagtaaggaa aaataaattc ttcaaaagta gagattacaa ggcttgttta
    38641 agaaagaatt caaagacctt gacaaataaa aataaaatgg ttattataaa aaatggtctg
    38701 aaatagatga tgatactttt cgaaaatctc ttcaaactac gtcagctcag ctttgccttg
    38761 ctgtgttttg agcaagctac tgttagcttc cgagtttgat tttcatttac tagaaatgaa
    38821 actgatgaga gatatcagta gacatttgag tcaggatatt atggaaaatg ataaaaagag
    38881 ctcgtgagat tggcatatca gactactaaa gtattgagtt tgttaggatt ttagcgacta
    38941 gttagctggg aaaggaagat atttgtgaca aataataaac tgtattcgtt gatagaattt
    39001 agaaataaaa tatatgaaga attagaactt tccagaagtg atttagcgat tttactatgt
    39061 gccatgctta tcgcctctat cggattaaat atggattcga ctcccgtgat tattggagcc
    39121 atgttaatct ctcctttgat gacacctatt ctgggagtgg ggctctctct agctatattt
    39181 gattttaaat tgttaagaaa atcttttaaa atattagcta ttcaaattct tgccagtcta
    39241 atagcttcaa cactttattt ttatctttct cccatttcgt atgctagttc ggagattgtt
    39301 gctagaacct ctccgactat ttgggatgtt ctcattgctt ttgtaggagg gatagcaggt
    39361 atcattggtg ctaggaaaaa agagaccaat aatattgttc ctggtgttgc tattgcaacc
    39421 gccttgatgc ctcctctttg tacagtaggt tatgctattg cttctgctaa tctaaaattt
    39481 atcataggct cctcttacct attcctcatc aattgtagct ttattgtcat tgcgacttat
    39541 ataggtgtta ggttgatgat ggttaagaaa cattatttta aagataatga agaagactct
    39601 aaaatgcgta ggattttgct tctagttgct gttttgctga tgattccgag tttcatctct
    39661 gcaacgactt tagtgagaga aacgttgaaa aaagagtccc ttaagaaatt tatatcagag
    39721 cagtttcagg ggtataatat tttgaaaaaa acctattcta aaaagactca taccctaaag
    39781 ctaaccattt caggaaatta tttgacagaa gaagaactcg atatgatttc aagtaagaga
    39841 ggtgactatg gtttaagtga tgtttctgtt caagtttcac aattgtctga ttcagaacaa
    39901 cttagtaagg aagagctggt ggagtatttc ttccagtata tcaaggataa ggaagcaaaa
    39961 gaaaaggaga aagccaataa atttgataca gagtctgagg agcaataatt tcttgagaat
    40021 agctggtttc tctcgtgagt cttctatgta tatcaaagga agactgaggt tttaagtata
    40081 aaacttttct tctattatag tagctaagtc ttgacagggt tcaaaaaatg gtttacactt
    40141 tataaagttt attactttga aaaggtgtga tactgtggct acggcaacaa aaaagaaaaa
    40201 atcaacagtt aaaaaaaatc tagtcatcgt ggagtcgcct gctaaggcca agacgattga
    40261 aaaatatcta ggcagaaact acaaggtttt agccagtgtc gggcatatcc gtgatttgaa
    40321 gaaatccagt atgtccgtcg atattgaaaa taattatgaa ccgcaatata ttaatatccg
    40381 aggaaaaggc cctcttatca atgacttgaa aaaagaagct aaaaaagcta ataaagtttt
    40441 tctcgcgagt gacccggacc gtgaaggaga agcgatttct tggcatttgg cccatattct
    40501 caacttggat gaaaatgatg ccaaccgtgt ggtcttcaat gaaatcacca aggatgcagt
    40561 caaaaatgct tttaaagaac ctcgtaagat cgatatggac ttggtcgatg cccaacaagc
    40621 tcgtcggatc ttggatcgct tggtagggta ttcgatttcg cctattttgt ggaagaaggt
    40681 caagaagggc ttgtcagcag gtcgcgttca gtccattgcc cttaaactca tcattgaccg
    40741 tgaaaatgaa atcaatgcct tccagccaga agaatactgg acagttgatg ctgtctttaa
    40801 aaagggaacc aaacaatttc atgcttcctt ctatggagta gatggtaaaa agatgaaact
    40861 gaccagcaat aacgaagtca aggaagtctt gtctcgtctg acgagtaaag acttttcagt
    40921 agatcaggtg gataagaaag agcgcaagcg caatgctcct ttaccctata ccacttcatc
    40981 tatgcagatg gatgctgcca ataaaatcaa tttccgtact cgaaaaacca tgatggttgc
    41041 ccaacagctc tatgaaggaa ttaatatcgg ttctggtgtt caaggtttga ttacctatat
    41101 gcgtaccgat tcgactcgta tcagtcctgt agcgcaaaat gaggcggcaa gcttcattac
    41161 ggatcgtttt ggtagcaagt attctaagca cggtagcaag gtcaaaaacg catcaggtgc
    41221 tcaggatgcc catgaggcta ttcgtccgtc aagtgtcttt aatacaccag aaagcatcgc
    41281 taagtatctg gacaaggatc agcttaagct atataccctt atctggaatc gttttgtggc
    41341 tagccagatg acagcggccg tttttgatac catggctgtt aaattgtctc aaaaaggggt
    41401 tcaatttgct gccaatggta gtcaggttaa gtttgatggt tatcttgcca tttataatga
    41461 ttctgacaag aataagatgt taccggacat ggttgttgga gatgtggtca aacaggtcaa
    41521 tagcaaacca gagcaacatt tcacccaacc gcctgcccgt tattctgaag caacactgat
    41581 taaaacctta gaggaaaatg gggttggacg tccatcaacc tacgcgccaa ccattgaaac
    41641 cattcagaaa cgttattatg ttcgcctggc agccaaacgt tttgaaccga cagagttggg
    41701 agaaattgtc aataagctca tcgttgaata tttcccagat atcgtaaacg tgaccttcac
    41761 agctgaaatg gaaggtaaac tggatgatgt cgaagttgga aaagagcagt ggcgacgggt
    41821 cattgatgcc ttttacaaac cattctctaa agaagttgcc aaggctgaag aagaaatgga
    41881 aaaaatccag attaaggatg aaccagctgg atttgactgt gaagtgtgtg gcagtccaat
    41941 ggtcattaaa cttggtcgtt ttggtaaatt ctacgcttgt agcaatttcc cagattgccg
    42001 tcatacccaa gcaatcgtga aagagattgg tgttgagtgt ccaagctgtc atcagggaca
    42061 aattattgag cgaaaaacca agcgtaatcg cctattctat ggttgcaatc gctatccaga
    42121 atgtgaattt acctcttggg acaagcctgt tggtcgtgac tgtccaaaat gtggcaactt
    42181 cctcatggag aaaaaagtcc gtggtggtgg caagcaggtt gtttgtagca aaggcgacta
    42241 cgaggaagaa aagattaaat aagaggaggg gcccgaaagt aaatttcagg ctccttttgg
    42301 ttagaacttg acaaaaaaca tcattttatg cgaaactaga agaagattat tttatactca
    42361 atgagaatca aagagcaaat tagaaagcta gtcgcaggtt gctcaaagca ctactttgag
    42421 gttgaagatg gaagctgacc tggtttgaag agattttcga agagtattaa ctaggagaag
    42481 ttatgcgtat tatctatcta attattggtt ttttatcgct gaccttggct attgttgggg
    42541 ttgttttacc cttgttgcct acaacacctt tccttttgtt gtctattgct tgtttctcca
    42601 gaagttccaa gcgattcgaa gattggcttt atcataccaa gctctatcaa gcatatgtag
    42661 ctgattttcg tgagaccaag tctattgcgc gtgaacgaaa gaaaaaaatc atcgtctcta
    42721 tctacgtctt gatgggaatt tctatttatt ttgcacctct tttaccagtc aaaatcggtc
    42781 tgggtgcttt gaccatcttt attacttatt atctcttcaa ggtcattcca gacaaagaat
    42841 agttaaaaca gtagttattt gccttgataa aattgaaagc atattcataa caatatgata
    42901 taataaaatt gaagtaatat tcaaggagaa tcaaatgatt tacgaatttt gtgctgaaaa
    42961 tgtgacttta cttgaaaaag cgatgcaggc tggagctcgt cggattgaac tctgtgataa
    43021 tctagcagtt ggtgggacaa cacccagcta tggagtgact aaggcagcgg ttgaactggc
    43081 agctaactac gatacaacca tcatgaccat gattcggcca cgtggtggtg actttgtcta
    43141 taatgaccta gaaattgcta tcatgctaga agacattcgt ttgactgctc aggctggaag
    43201 tcaaggggtt gtatttggag ctttaactgc tgataaaaag ttggataagc ctaatctgga
    43261 aaagttaatt gctgcatcaa aaggaatgga aattgtcttt cacatggcct ttgatgaact
    43321 aagtgatgaa gatcaagcgg aagctattga ctggctcagt caagccggtg tcactcgtat
    43381 cctaactcgt gctggtgtgt ctggcgactc cttagaaaaa cgttttgttc actatcacag
    43441 aattttggag tacgctaaag gtaaaattga aattctacca ggtgggggga ttgaccttga
    43501 aaaccgtcaa acctttatcg accaggtggg ggtaacacaa ttgcatggta ctaaggttgt
    43561 tttttaaaaa atagaaagga actgctagct ttgggtagca gttttcactt atgtttgaaa
    43621 tttttaaatc ctatcaattt aatcaagaaa aggctcatga ttatggtttt atagaaaata
    43681 gcgaagtctg gacatatagt tgccagattt tgcaaggtga ctttgtcatg actgtgtcca
    43741 tcactgctga taatgtgaac tttcaagtct ttgaccaaga gactggtgac ctctatcctc
    43801 acgtttatat ggaaagcatg aggggaagtt ttgtcggaaa tgtccgtgag gcttgtctgg
    43861 agattcttta ccagattcgg aaggcttgtt ttgatgtgca agattttatc tgtcatcaga
    43921 ctaagcgtat catgactcaa gttcaggaaa agtatggaaa ccagttggag tatctgtggg
    43981 aaaaatcgcc tgatacagct gtattgcgcc atgaaggcaa tcaaaagtgg tatgccgtct
    44041 tgatgaaaat ctcttggaat aagctggaaa agggcagaga aggacaagtg gaagcagtca
    44101 acctcaagca tgaccaagta gctaatttgc tttcacaaaa ggggatttat ccagccttcc
    44161 atatgagcaa gcgctactgg attagtgtgt cccttgatga tactttatca gatgaagaag
    44221 tactggaatt gatagaaaaa agttggaact taacctctaa aaaatgaaat attttaataa
    44281 ttttcatgaa ctttcaatta gctaaatatt ctttactgaa gagattttta gaaaatatag
    44341 gatttaccac actagaggaa tatggtgcca tcttcaaata cctgattgag aatgtcaaga
    44401 cggatcgtca gatcatctat tcgcctcact gtcatgatga cctcggaatg gcagtggcaa
    44461 atagccttgc tgctgtcaag aatggtgcag gacgtgttga agggactatc aatggtatta
    44521 gggagcgagc tgaaaatgct gctttggaag aaattgcagt ggctctcaat attcgccaag
    44581 attactacca agtagaaacc agtattgtcc taaatgagac catcaatacg tcagaaatgg
    44641 tttctcgctt ctctggtatt ccagttccta aaaacaaagc cgtcgttggt ggcaatacct
    44701 tctcccacga atctggtatt caccaagatg gagtccttaa aaatcctctc acttatgaga
    44761 tcatcacacc tgaattggtt ggtgttaaga ttctgcttgg aaaattatct ggtcgccatg
    44821 cttttgttga gaaactgaga gaattggccc tagattttac agaagaggat atcaaaccac
    44881 tctttgctaa gttcaaggca ctggtcgata agaagcaaga aatcacagat gcagatattc
    44941 gagctttggt agctggaacc atggttgaaa atccagaagg cttccacttt gatgatttac
    45001 aacttcaaac tcatgcagat aatgacattg aagcgctcgt tagcctagcc aatatggatg
    45061 gtgagaaagt cgaatttaat gcgacagggc aaggttccgt tgaagcaatc tttaatgcta
    45121 tcgataagtt ctttaaccaa tctgttcgtt tggtgtccta cactatcgat gcggtaacag
    45181 atggaatcga tacccaggat cgggttttgg tcactgttga aaacagagat acagaaacca
    45241 tctttaatgc agcagggctt gattttgatg tgttgaaggc ttctgctatt gtctatataa
    45301 acgctaatac ctttgttcaa aaagagaatg caggtgagat gggacgcagt gtttcttacc
    45361 acgatatgcc tagtgtgtaa aggagaaggc tatggcaaag aaaatagtag ctctagcagg
    45421 agacggaatt ggcccagaaa tcatggaggt tggtttagaa gttctggagg ctctagctga
    45481 aaaaacaggt tttgactatg agattgacag acgaccgttc ggaggtgcag atattgatgc
    45541 agcatgacct cccttacctg atgaaaccct taaggcaagt agggaagcag atgctatcct
    45601 actagtagct atcggtagtc ctcagtatga tggagcagtg gttcgccctg aacaaggcct
    45661 gatggctctc cgtaaggaac tcaatcttta cgctaatatt cgtcctgtaa aaatctttga
    45721 cagtctcaag catttgtcac cactcaaact ggaacgaatt gctggtgtag actttgtcgt
    45781 ggtgcgtgaa ttgacaggcg ggatttactt tggatatcat attcttgaag agcgcaatgc
    45841 gcgtgatatc aacgactata gctatgagga agtggagcgg attattcgca aagcctttga
    45901 aattgcaaga aatcgcagaa aaatcgttac tagtatcgat aagcaaaatg ttctagcgac
    45961 ctcaaaactc tggcggaaag tagctgagga agtcgcacag gatttcccag atgtaacctt
    46021 ggaacatcag ctggtagact cagctgctat gcttatgatt accaatcctg ctaagtttga
    46081 tgttattgta acggagaatc tttttggaga tattttatct gatgaatcaa gcgtcttatc
    46141 tggtacactt ggggttatgc catcagccag tcattctgaa aatggaccaa gtctctatga
    46201 acctattcac ggttcagcac ctgatattgc aggtcaagga attgccaatc ctatttccat
    46261 gattttatca gtttccatga tgttgagaga tagtttcgga cgttatgagg atgcagagcg
    46321 tatcaaacgt gctgttgaga caagtctggc ggcaggaatt ttaacgagag atataggagg
    46381 tcaggcttca acaaaggaaa tgacggaagc tattattgca aggttatgaa gttagacgaa
    46441 aaaattactc tagtcctttt gatttggaat gtcatcattt tcttgattta tggtattgac
    46501 aaatctaagg caaggagaag agtttggcgc atccctgaga aaatcttact tattttagcc
    46561 tttacttttg gtggttttgg tgcctggcta gcaggaatca tctttcacca caagactcga
    46621 aaatggtact ttaaaatagt ttggtttctt gggatggtga ccacactagt agccttatat
    46681 tttatttgga ggtaatggat ggcagggtct tcgagggaat acgctgcttg ggctctagcg
    46741 gactatggtt ttaaggtcgt gattgcagga tctttcggtg acattcatta caataatgaa
    46801 ctcaataatg gcatgttgcc aatcgttcag cctagagagg ttagagagaa actagcccag
    46861 ctaaaaccaa ccgaccaggt aactgtggac ttggaacaac aaaaaatcat ctcaccagtt
    46921 gaagaattca ccttcgagat agatagcgag tggaaacata aactcctaaa tagtttggat
    46981 gatatcggta ttaccttgca gtatgaagag ttgattgctg cttatgaaaa acaacgacca
    47041 gcctactggc aggattagaa aaaatagaaa aggagatata gtaaactgaa ataagatgta
    47101 aacaaatgaa ttggaactta acatccattt ccagcaattt tttagaaact acagtggact
    47161 attctggatt caacacatta taaaattatg acaaaacaca ttcacaagaa ggctacgaca
    47221 ttttaaaagg tgagggcgga tgtatcgttt gccctactaa agttggttac attatcatga
    47281 ccagtgacaa ggcaggactt gagcgtaagt tcgcagccaa agaacgtaag cgtaacaaac
    47341 caggtgttgt tctctgcggt agcatggatg aactttgcgc tttagcgcaa ctcaacccag
    47401 aaattgaagc attctactaa aaacattggg atgaagatat tcttcttggt tgtatccttc
    47461 cttggaaacc agaagccttt gaaaaactca aagcatacgg ggatggccgt gaagaactta
    47521 ttactgatgt acgtggtact agctgttttg ttatcaagtt tggaaaagca ggtgaacaat
    47581 tggctgccaa gctttgggaa gaaggtaaaa tggtctacgc ctcatctgct tcaatgacaa
    47641 aacgattgaa actcgctatg agcaaggtgt aatggtgtct atggtcgata aggacggcaa
    47701 actcatccca gaacaaggag gagcacgttc aacttcacca gctccagttg tgatccgtaa
    47761 agggcttgac attgataaaa tcatgatgca cctgtcagat acttttaact catgggacta
    47821 ccgtcaggtt gagtattatt aggatagaga agaagtctag tgttatgaga tattaaagct
    47881 cctaacactg ggcttttgtt tagaatttct tttctttttc tataggatat ggtattctat
    47941 gtagaaaata tatgttaata agtaatgcca atatttaaac atcattagta aaaggagtta
    48001 gattgatgaa taaaagaaaa gttagtttag aagattttta taaatggtat agtctaaata
    48061 aagaagagtt attaaataag gcaactgttg gtgaaaagtt taatgataaa ttaaaagaag
    48121 agtttctcca ggaatggcct ttggatagga ttttaacaat gtcaatcgat gaatatgtaa
    48181 taggaaaggg acagcaaaat aagtctttat gctacgctct tgagaaggga aaatacaaaa
    48241 atctatttct tggaatttct ggtggctcag cttcaaaatt tggtatttat tggaataaaa
    48301 aaacaaacaa atataaagat caagctaata atgagatttc agagttggat cagcgatttt
    48361 caaaattaaa atcagatttg tatgaaatta tcaaagaagg tattcgtttt aactttgaaa
    48421 atcctatttt tgatatgaaa agatcaacaa atgaatttat tggtcgttct gctatggtga
    48481 caaaattact ttgtatctat actgagggag atcctttctt tggtgtaaat attaatagtc
    48541 agaaagaatt ttggaaccac tttgtttctc agacaaatca aggtggacct tatctgcaaa
    48601 atcataaaat aattgaactg gtgtccaaaa cttatcctga gttggagcca tcgaaattag
    48661 gaactatgct ttttgagtat tctaagcttt ttatggaaaa taaggaagac aatagtacaa
    48721 tggattcatc aaacaatttt cgtcatcaat taactcaatc tctattaaag tctccaaacc
    48781 tcatcctccg cggtgctcct ggcacgggaa aaacttatct tgctaaagaa attgctaaag
    48841 aattaacgga tggcaacgaa gatcaaatcg gatttgtaca atttcaccca tcatatgatt
    48901 atacggattt tgtagaaggt ttaagaccag tatcaaatgg ggatggagct attgagttta
    48961 ggctacagga cggtattttt aaagattttt gtcagaaagc aaaagaaacc caattgattg
    49021 gaggacaaga taattttgat gaggcttggg attcttactt agaatatata aatgttgctg
    49081 aagaaaaaga atatataaca aaaacatctt acttatctgt taatagtaga caaaatttgt
    49141 cagtaaatta tgatagtggt gttccaggat ggtcactacc tagcaaatat gtttacgagt
    49201 tgtataaaga taaaaattat aataagcaag aatactacaa aagtggtgga aaaactgtcc
    49261 tagaaacatt gagaaagaga tttggtttga aagactatgt ttccccaaca gaaattgata
    49321 ctgataagaa attcgtcttt atcatcgatg aaatcaatcg tggggagatt tctaagattt
    49381 ttggcgaact ctttttctct atcgaccctg gctatcgtgg tgaaaaagga agtgtttcta
    49441 cccaatatgc aaatctacac gaaactgatg aaaagttcta tatccccgaa aatgtttaca
    49501 tcatcggaac tatgaatgat attgatcgtt cagtggatac ctttgatttt gctatgcgtc
    49561 gtcgttttcg ttttgttgaa gttactgccg agggtcaagt tggcatgttg gataaagagc
    49621 tgaatatcca tgcagaagaa gcaaaaattc gtctaagaaa cttgaacgct gctatcgaaa
    49681 atgttcagga attaaacagt cattatcata ttggaccaag ttattttctt aagttgaagg
    49741 atgtagattt tgactatgaa ttactctggt ccgattatat taagcctctc ctagaagact
    49801 acttgcgagg ttcttatgat gaggttgaaa ctttggaaac tttgaaaaaa gcatttgaac
    49861 tgacaaataa tgagcaaaaa gatcaggcag tagctgatga caatgaaggt gatgaaaacg
    49921 atgatgcaga ttactgataa tcaacacaag attattaaag aaaaatttgt tgaagaatat
    49981 cctaaactaa gcaatcttct tttagacaga accttggaaa gtctatccca agatgaacgt
    50041 attttcattt ttccaaatga tttgactcat actcctgatt tggataagga ccaaaagatt
    50101 tttgaaacag tcaatcagaa aatcaagaca gggaacgtga ttggttttct tggatatggt
    50161 caggaaagat taacgatttc ctcacgattt tctgatgaga gtaatgacca ctttttgcat
    50221 tatctcttaa acaaggttct tcatatcaat ctcactagtt tagatgttgc tttgtctcgt
    50281 gaagagaggc tttatcaact tttggtgtat ctctttccca agtatctaca agctgctatt
    50341 cgaaaaggtc tttataagga atatcatcga ttttctcata acgacagtca tgttaaggga
    50401 gtgattgatg taagaaacca tctcaagaaa aatcttcctt tcacgggaaa tattgcctac
    50461 gcaacgagag agttcaccta tgataatccc ctcatgcagt tggtccgtca cactattgaa
    50521 tacattaaga atcagaaaag cattggtcaa ggggtactag ataatctctc aactagtcgt
    50581 gaaaacgtat ctgaaatcgt gcgtgtaacg ccctcttata aactagctga tcgtgctaag
    50641 attattcggg gaaatcaatc taaacctata cgtcatgcat actttcacga gtacagaaac
    50701 ttacaagaac tttgtctgat gatcctaaac caagaaaagc acggtttagg gtatcaagat
    50761 caaaaaatct atggtattct ctttgatgtt gcctggcttt gggaagagta tgtttacacc
    50821 ttgttgccaa aaggttttgt acatcccaga aataaggata agacggatgg aatttcagta
    50881 ttttctgttg ggaaacgaaa agtatatcca gatttttatg acagagaacg aaagattgtt
    50941 ctagatgcaa aatataaaaa actggaattg actgaaaaag gaatcaaccg tgaggactta
    51001 ttccagctga tttcctattc ttatatttta aaagctgaga aggctggact gatttttcct
    51061 agtatggagc agtcagtaaa tagtgaaata ggaaaagtag ctggctatgg agctcaattg
    51121 aagaagtggt ctattcgaat ccctcagaat gcctcattct atagtacatt ttgtaaaatg
    51181 atggaaaatt cagaagaaaa ttttaaagcg attattgatg aagaagtggg gagaaaataa
    51241 tttcccctct ttcttttatt tttaacacaa gtaaaacccg aacgatatat tttaattcat
    51301 aaaataattt gacaaaaact gtactttgat ttataataaa ttaaggaaac gtcggaaaag
    51361 gcgtagtgat gcaagagcat aggtaggtca ttacaaaaga aacgagacat cgatatgtta
    51421 aatgaatttc caatttttga ttacgaagat attcaattga ttccaaataa atgtgtcatt
    51481 aaaagccgtg cagaagcgga tacaagtgtt actttaggaa atcacacctt taaactacct
    51541 gttgtgccag cgaatatgca gacgattttg gatgaaaatg tagcagagca actggctaaa
    51601 ggtggttact tctacattat gcaccgtttt gatgaggcag gacgcattcc ttttattaaa
    51661 cgaatgcaca atcaagggct cattgcttct atctctgtcg gtgttaagga ttatgagtat
    51721 gattttgtta gccagctcaa ggctgatact cctgagtata tcactattga tattgctcat
    51781 ggtcatgcgg atagcgtgat ttctatgatt caacacatca agaaagaatt gccagatacc
    51841 tttgtcattg ctggaaatgt gggaacacca gaagctgtgc gtgaattgga aaatgctggt
    51901 gcggatgcta ctaaggtcgg aatcggtcct ggtaaggttt gtatcaccaa ggttaagact
    51961 ggttttggta caggtggttg gcagttggct gctctacgct ggtgtgccaa ggctgcgcgt
    52021 aaaccgatta tcgctgatgg aggaattcgt actcacggtg atattgctaa gtctatccgc
    52081 ttcggtgcta gcatgatcat gattggttcc ctctttgcag gacacattga aagtccaggg
    52141 aaaacgattg aagtcgatgg tgaacagttc aaagaatatt atggttcagc ctcacaatat
    52201 caaaaaggtg cttacaaaaa tgtggaaggc aaacgtatct tgcttcctgc taaaggtcat
    52261 ctgcaagaca ctttaactga gatggaacaa gaccttcaaa gtgctatttc ttatgcaggt
    52321 ggacgtcagg ttgctgacct taaacacgtt gattatgtga tcgtgaaaaa ctccatctgg
    52381 aatggggatg cttcccacta agacggggtc tttgaccaaa aaacttgtta ttagagcaaa
    52441 tttctgttat aataaaacaa gtttccaccc ttagtgtaat ggatatcacg taagattccg
    52501 gttcttgaga tgggggttcg attccctcag ggtggatgta aacatcctaa aaagccttta
    52561 atagggcttt tttctttatc ctgacccaaa tttgccgcta aaactgagaa tctctgtcta
    52621 aagaattccc aaatgctttc cagtcccgtt ttagagtgac tcagggggct ttttttgata
    52681 taataaaaag gactattatc agttagaaag aggttggtat gaaagaatta caaactgtac
    52741 taaagaatca ttttgcaatc gaatttgcag acaaaaattt actggaaact gcctttactc
    52801 atacgagtta tgccaatgag caccgcctct taaaaatttc acacaatgaa cgcttggaat
    52861 ttttaggaga cgctgttcta cagttattga tttcagaata tctatataaa aaatatccta
    52921 aaaagcctga aggtgaccta tcaaaactcc gtgctatgat tgtccgtgag gagagtttag
    52981 ctggttttgc gcgtgattgc cagtttgacc agtttatcaa gttgggtaaa ggggaagaaa
    53041 aatcaggtgg tcgcaatcgt gacaccattc ttggtgatgc ctttgaagcc tttcttggtg
    53101 cccttctttt ggataaggat gtggccaagg tcaaggaatt tatctatcaa gtcatgattc
    53161 ctaaggttga agcaggcgag tttgagatga ttacagacta taaaacccat ctccaagagt
    53221 tgcttcaggt caatggtgat gtggctattc gttatcaggt gatttctgaa acagggcctg
    53281 ctcacgataa ggtttttgat gtagaagttc ttgttgaagg taagagcatc ggtcaaggcc
    53341 aaggtcgttc taagaaatta gcagagcagg aagctgccaa aaatgccgtt gagaaggggc
    53401 tggattcatg tatttaaagg aaatcgaaat tcaggggttc aagtcttttg ctgataagac
    53461 caaggtcgtt tttgaccaag gtgtgacggc agttgttgga cccaatggat ctggaaagtc
    53521 caatattaca gaaagtctgc gttgggcttt gggggagtct agtgtcaaga gtctccgtgg
    53581 gggcaagatg ccggatgtca tctttgctgg aaccgaaagt cgcaaaccgc tcaattatgc
    53641 ttctgtagtt gtgactctgg ataatcatga cggatttatc aaggatgcag gtcaagaaat
    53701 cagggtggaa cgccatgtct atcgtagtgg agatagcgaa tacaagattg acggcaagaa
    53761 agtccgtctg cgtgatattc atgacctctt cttggatact ggattgggac gagattcctt
    53821 ctctattatt tcccaaggga aggttgagga gatttttaat tccaagcctg aggaacgacg
    53881 agctattttt gaagaagctg ctggagtttt aaaatacaag actcgcagaa aagaaaccga
    53941 gagtaaactg cagcaaactc aggataatct ggaccgctta gaggacatta tctacgagtt
    54001 ggataatcaa atcaagcctc ttgagaagca agctgagaat gcccgtaagt ttttagactt
    54061 ggaaggacaa cgtaaggcta tttatttaga tgttctggtt gctcaaatca aggaaaataa
    54121 ggctgaacta gagtcgacag aagaagagtt gactcaggtt caagaactct tgatgagtta
    54181 ttaccaaaag cgtgaaaaat tagaagaaga aaatcaaact cttaaaaagc aacgccaaga
    54241 tttacaggct gaaatggcca aagaccaagg cagtttgatg gacttgacta gtctgattag
    54301 tgatttagaa agaaaattag ccctatcgaa actggagtcc gagcaagtgg ccttgaatca
    54361 acaggaggca caagcccgtt tggctgcttt ggaggataag agaaattcac tcagcaaaga
    54421 aaagtctgat aaagaaagct ctttagctct gttagaggga aatctagtcc aaaataatca
    54481 aaaactcaat cgtttagaag ctgaattgct ggctttctca gacgatcctg atcagatgat
    54541 tgagctctta cgtgaacgct ttgtagctct tttacaagaa gaagcggatg tctcaaacca
    54601 gttgacccgt attgagaatg agttggaaaa tagtcgtcag ctttctcaaa aacaagcaga
    54661 tcaactagaa aagctgaaag agcaattagc tatagctaaa gagaaggcta gtcagcaaaa
    54721 ggaagagctt gaaactgcca aggagcaggt tcagaaatta ttggctgact accaagctat
    54781 tgccaaggag caagaggagc agaaaacttc ctatcaagct caacaaagcc aactctttga
    54841 ccgtctggat aatctcaaaa acaagcaggc cagagctcaa agtttggaaa atatcctgag
    54901 aaatcatagt aacttttatg caggtgttaa gagtgttctc caagaaaaag atcgcctagg
    54961 tgggattatt ggtgcagtca gtgagcatct gacctttgat atgcattatc aaactgccct
    55021 agaaattgcg cttggagcga gtagccaaca tatcatcgta gaagatgaag agtcggcaac
    55081 caaggcgatt gatttcctta aacggaacag agccggtcgt gcaacctttc ttcctttgac
    55141 cactatcaag gcgcgtacga tttctagtca gaaccaagat gctatcgctg taagcccagg
    55201 tttccttggg atggcagatg agttggtgac ttttgatact agactggaag ccattttcaa
    55261 gaacttgcta gctacgacgg ctatctttga taccgtagaa catgcgcgtg cagcagcgcg
    55321 ccaggttcgt tatcaggttc gtatggtgac attggacgga acagagctgc gcacaggtgg
    55381 ttcttatgca ggtggagcta atcgccagaa taacagtatt ttcatcaagc cagaactgga
    55441 gcaattacaa aaagaaattg ctgaagaaga agcaagcttg ggttcagaag aggcgacttt
    55501 gaagacctta caagaccaga tggctagatt gtccgaatta ttagaagcca tcaaatctca
    55561 aggagaacag gcacgtattc aggagcaagg cttgtccctc gcttatcagc agaccagtca
    55621 gcaagttgaa gagctagaaa ctctttggaa actccaagaa gaggaattag atcgtctttc
    55681 tgacggagat tggcaagcag ataaggaaaa atgccaagat cgtcttgcta ctatcgccag
    55741 tgacaagcaa aatctggaag ctgagattga agagattaag tctaacaaaa atgccatcca
    55801 agaacgctat caaaacttgc aggaagagct agcgcaagct cgtttgctta agacagaact
    55861 gcaagggcaa aaacgttatg aagttgctga tattgaacgc ttaggcaagg aattggacaa
    55921 tcttaatatc gaacaagaag aaattcagcg catgctccaa gaaaaagttg acaatcttga
    55981 gaaggttgat acagaattgc tcagtcaaca ggcggaagaa tccaaaactc agaaaacaaa
    56041 tctccaacaa ggtttgattc gcaagcagtt tgagttggat gatatagaag gtcaactgga
    56101 tgatattgcc agtcacttgg atcaagctcg ccagcagaat gaggagtgga ttcgcaagca
    56161 aacacgtgct gaagccaaga aagaaaaggt cagcgagcgc ttgcgccatc tacaaaatca
    56221 attaacagac cagtaccaga ttagctatac tgaagcacta gaaaaggcac atgaattgga
    56281 aaacctcaat ctggcagagc aagaggtgca ggatttagag aaggctattc gctcattggg
    56341 acctgtcaac ttggaagcta ttgaccagta cgaagaagtt cacaaccgtc tggactttct
    56401 aaatagtcag cgagatgata ttttgtcagc gaaaaatctg ctccttgaaa ccattacaga
    56461 gatgaatgat gaggtcaagg aacgctttaa atcaaccttt gaagctattc gtgagtcctt
    56521 taaagtgacc ttcaagcaga tgtttggcgg aggtcaggca gacttgatat tgactgaggg
    56581 cgacctttta acagctggtg tggagatttc tgttcaacct ccaggtaaga aaatccagtc
    56641 gcttaacctc atgagtggtg gtgaaaaagc tctatcggct cttgccttgc ttttctccat
    56701 tattcgtgtc aagaccattc cttttgtcat cttggatgag gtggaagctg cgctggatga
    56761 agccaatgtt aaacgttttg gggattacct caaccgcttt gacaaggaca gccagtttat
    56821 cgttgtaacc caccgtaagg gaaccatggc agcggctgat tccatctatg gagtgaccat
    56881 gcaagaatcg ggtgtttcaa agattgtttc agttaagtta aaagatttag aaagtattga
    56941 aggatgacaa ttaaactagt agcaacggat atggacggaa ccttcctaga tgggaatgga
    57001 cgctttgata tggatcgtct caagtctctc ttggcttcct acaaggaaaa agggatttac
    57061 tttgcggtgg cttcgggtcg gggatttctg tctctggaaa aattatttgc tggtgttcgt
    57121 gatgacatta ttttcatcgc ggaaaatggc agtttggtag agtatcaagg tcaggacttg
    57181 tatgaagcga ctatgtctcg tgacttttat ctggcaactt ttgaaaagct gaaaacgtca
    57241 ccttatgtag atatcaataa actgctcttg acgggtaaga agggttcata tgttctagat
    57301 acggttgatg agacctattt gaaagtgagt cagcattata atgaaaatat ccaaaaagta
    57361 gcgagtttgg aagatatcac agatgacatt ttcaaattta caaccaactt cacagaagaa
    57421 acgctagaag ctggtgaagc ttgggttaat gaacatgtcc ctggtgttaa ggccatgaca
    57481 actggctttg aatctattga tattgttctg gactatgtcg ataagggtgt agctattgtt
    57541 gaattagcta aaaaacttgg tatcacaatg gatcaggtca tggcttttgg agacaatctt
    57601 aatgacttac atatgatgca ggttgtggga catcctgtag ctcctgaaaa tgcacgacct
    57661 gaaattttag aattagcaaa gactgtgatt ggtcaccata aggaacggtc ggttatagct
    57721 tatatggagg gcttataatg gcagatataa aattgattgc attggacttg gacgggacct
    57781 tgctgactac tgataaaagg ctgacggatc gtaccaagga aaccttgaaa gctgcgcgtg
    57841 atcgtggtat caaggtcgta ttgacaactg gtcgtccctt aaaagccatg gatttctttc
    57901 tccatgagtt agggactgac ggtcaggaag atgagtatac cattactttt aatggtggat
    57961 tagttcagaa aaatacagga gaaatccttg ataaaacagt cttttcatat gatgatgtgg
    58021 cacgtttgta tgaagaaaca gagaaattat cactgcctct tgatgccatc tcagaaggaa
    58081 cagtttatca aatccaatcg gaccaagaaa gtctttatgc caaattcaat ccagctttga
    58141 cctttgttcc agtggacttt gaagacttat ctagtcaaat gacctacaac aaatgcgtga
    58201 ctgcctttgc tcaagaaccc ttggatgcag ccattcagaa gatttctcca gaattgtttg
    58261 accaatatga aatctttaaa tcacgtgaaa tgttgctaga atggtcacca aagaatgttc
    58321 ataaagcaac aggtttggca aaactaatca gccatcttgg aatcgaccaa agtcaagtga
    58381 tggcttgtgg tgacgaggcc aatgacctct ctatgattga atgggcaggt cttggtgttg
    58441 ctatgcaaaa cgctgttcct gaagtaaagg tagccgcaaa tgtagtgacg ccgatgacca
    58501 acgatgagga agctgtcgcc tgggctatcg aagaatatgt gctaaaggag aactaagata
    58561 tgggattgtt tgaccgtcta ttcggaaaaa aagaagaacc taaaatcgaa gaagttgtaa
    58621 aagaagctct ggaaaatctt gatttgtctg aagatgttga tcctaccttc acagaagttg
    58681 aggaagtttc tcaggaagaa gcagaggttg aaattgttga acaagctgtg ttccaagaag
    58741 aggaaatcca agacacagtt gaagaaagtc tggatttaga gccagttgta gaagtttctc
    58801 aaaaagaagt cgaagaattt ccacactcag aagaagggaa tactgagttt ttagagactg
    58861 tagaagaaaa taattctgaa attcttgaag cagaaaggcc tcaaacagaa gaaactgttc
    58921 aggaaaagta tgaccgcagt cttaagaaaa ctcgcacagg tttcggtgcc cgcttgaatg
    58981 ccttctttgc taacttccgc tctgttgacg aagaattttt cgaggaactg gaagaactgt
    59041 tgattatgag tgatgttggt gtccaagtcg cttctaactt aacggaggaa ctacgttacg
    59101 aagccaagct tgaaaatgcc aagaaacctg atgcacttcg tcgtgtcatc attgagaaat
    59161 tggttgagct ttatgaaaag gatggtagct acgatgaaag catccacttc caagataact
    59221 tgacagttat gctctttgtt ggtgtgaatg gtgttgggaa aacaacttct atcggaaaac
    59281 tagcccaccg ctacaaacaa gctggtaaga aggtcatgct ggttgcagca gataccttcc
    59341 gtgcgggtgc agtagctcag ctagctgaat ggggccgacg agtagatgtt ccagtagtaa
    59401 ctggacctga aaaagctgat ccagccagcg tggtctttga tggtatggaa cgtgccgtgg
    59461 ctgaaggtat cgatattctc atgattgata ctgctggtcg tctgcaaaat aaggataacc
    59521 ttatggctga gttggaaaag attggtcgta ttatcaaacg tgttgtgcca gaagcaccac
    59581 atgaaacctt cttggcactt gatgcatcaa caggtcaaaa tgccctagta caggccaaag
    59641 aattttcgaa aatcacacct ttaacgggaa ttgttttgac taagattgat ggaactgctc
    59701 gaggaggtgt ggttctagcc attcgtgaag aactcaatat tcctgtaaaa ttgattggtt
    59761 ttggtgaaaa aatcgatgat attggagagt ttaactcaga aaactttatg aaaggtctct
    59821 tggaaggttt aatctaatca gaagcaaaaa tcctgcaagg cataaacttg caggaaattt
    59881 ttttattcta agcgaccatc ttgacgatag gtgatatctg gttgccaagt ccatttggca
    59941 ccgaattttt caagtaggtc aaagctggct tgaggtccca tgcttccagc tttatagtca
    60001 tgaagtgggg caccattttc agcccagagc ttttcaatac ggtcaatcaa cttccatgac
    60061 gcacaaactt catcccagtg gctaaagtta gttgagttgt tatttaggac atcataaatc
    60121 aatttttcgt atggttctgg agaagcacca gttgcagtcg catctgtacg gtaatcaagt
    60181 gagttaggag ccaagttaaa ttcttctcct acttgcttcc catttaggct aagagagaag
    60241 ccttctgttg gttgaatata gatggtcaaa atatttggag caagtggttc tccaaagata
    60301 gaatccattt gtttaaagac gatgttgaca tgggttcctt tttcagtcag tcgtttacct
    60361 gtacggaaaa agaaaggaac actacggaat cgatcgctgt ctacaaagaa ggcaccagat
    60421 gtaaaggttt cagttgttga ttctggattc acatttggct cgctacgata agagatgtat
    60481 ttcatgccat caatcttacc agagcggtat tgcccacgta taaagtgttc tttgagttct
    60541 tcatcagttg gatgatagag gtttttaaag accttaatct tttcagcacg aatctcgtct
    60601 tttgtgaagc ttgctggttt gtccatggcg aggagcgaaa gaagttgtag agtgtggttt
    60661 tggaccatgt cacggagggc accggattgg tcatagtagc caccacgttc ttctacaccc
    60721 aagcgctccg caaaggtaat ttgaacattg tcgataaaat ccttgttcca aacgttttca
    60781 aaaatcaagt ttgcaaagcg aactgcaaag atgctttgga tcatttcctt accaagataa
    60841 tggtcgatac ggaaaatttg ttcttcgtca aatgttgcta ggagttcgtc attcaacttg
    60901 cttgcagttg cgtaatctgt accaaatggt ttttcaacga tcaagcgctc aaaacctttg
    60961 ccatcgacaa tgttttcaga tttgaggtgt ttggcaatgg ttccaaagaa ctgaggtgcc
    61021 atagacaaga agaagagctt attgtgttca gcttggtatt tttcattaag ctcagcttgt
    61081 aattgacgca aagcaatata atgttccgaa tcattgacat catggctttg atagtagaag
    61141 tggctagcaa attcttgggc ttgctcggta ctatctgcca aatcaaggat ggactcgaca
    61201 actacagatt caaaatattc cttactccaa ggtctacggg cagttccaat aacggcaaag
    61261 tgcttggaaa gattgccgga tttatatagt ctaaaaaggg aagggtagag cttgcgttta
    61321 gccaggtctc cactcgcacc gaaaattgta acaataacct tagatgacat ctagctacct
    61381 aatttctatt tttattccta gtcttgctag gtatgaggaa acctcatttc ataaacttaa
    61441 ttctaacata attttccgaa aaatcaaaaa ttccaatacg aggtagaaaa actgcaagga
    61501 aatccttaca gtttgagttt agacgtttaa gaccttatct aagaactctt tcagacgagg
    61561 gtgttgtggg ttatcaaaga tttggtcagg tgttccgtct tcaaggaact cgccatctgc
    61621 agtaaagata acgcggttgg caacctgacg agcaaatccc atctcatggg ttacgataat
    61681 catggtcatg ccttgctcag ccaattcctt cataacgtta agtacgtctc caaccatctc
    61741 agggtcaagg gcagaagttg gttcatcgaa gagcatgatg tctggattca ttgctaggcc
    61801 acgagctatg gccacacgtt gtttttgacc acctgatagg ctatctggat tggcattagc
    61861 tttatctgct agtccaacct tttcaagcaa ctccattccc aatttctcag cttcttcctt
    61921 agtcatcaac ttgtgctcaa taggagcaaa ggtgatgttg tccaatacag acatatgagg
    61981 gaagaggttg aagtgttgga ataccatgcc gatattttca cggacgtggt caacattggt
    62041 tgttttttca gttaaatcat agccgttcac agtgatgtga ccgctagtga cttcttctaa
    62101 aagattgagg ctacggagga aagttgactt accagaacct gaaggaccga tgatacaaac
    62161 aacatctcct tcatagaact tagtcgtaat tccttttagg acttcatttt ttccatagtg
    62221 cttgtgtaaa tcatttacat caatttttaa ttttgccatt aacgaatcct cttttctaag
    62281 cgtttcgcta gtctagtcaa aagtgtgata attacaagat agaagatagc aaggattgca
    62341 tacatcttga aactttggta gttacgagca atgataatct taccagtttg gaagagttca
    62401 accaaaccga tagcagatac gatagttgta tctttaagag cgataacgaa ttggttgaca
    62461 aagtttggca acatcaattt agttgcttgt ggcaagataa tcttacgcat ggtttttcca
    62521 taagagatac ccaagcttcg gctggcttcc atttggccaa ctggaacggc ctgaatacca
    62581 ccacgaacga tttcagcgat ataagcagcc gcattgagtg agagggcaat ggttccagct
    62641 acaaagtcgt taattgggct ttgttggcct gtgatagact cgatgaagtt tggaattccc
    62701 cagaagatga aggctgcaag aatcatcaat ggaataccac gaataacgtc aacgaaaatc
    62761 tcagagatga cgcgaagaga tttgtatggg ctaacgctaa acataccgaa gataattccg
    62821 atgacaatgg caatagcaaa tgagataaga gctagagcaa gagtgatacc aagaccgcta
    62881 aggagttgtt tgtagttgtt ttgaagcaag ccccagagcg ttgtttcgtc aacagtactt
    62941 gttgaagcag ttgaagattc gctagctagg tatttgtcaa gaatcttttg gaattcaccg
    63001 tttgctttaa ggtttgcaag tccgttgttg aacatttcaa tcagttctgg atttgctcct
    63061 tttttaacgg caaaggctgt ttcaccgatt ggagttccag agattggagt tttcaatttt
    63121 tgaccttggc tgatagaata tttgagaaca ggttcatcat ccataacggc atcaatggca
    63181 ccagtgttta aactgtcata cattgaagaa ccatcagcaa aggttttgat tttgtagccg
    63241 tatttgcttt gattttctgt taggaaggtt tgagaagcag ttccgttttt aacaccgact
    63301 gtctttcctt ttagatcttc ataagaagca atattgcttg attctttgac accaagaatg
    63361 gtattagcag tgtagtatga ttctgagaag tcaaaagttg ccttacgagc atctgtgaca
    63421 gacataccag cgatgatacc atcggcttga ccagcttgga cagcactgat agcagcatca
    63481 aaaccagggt tggtgatttc aatttcaaaa ccttggtctt tagcgattgc cttaatcaat
    63541 tccatatcaa taccagtgta ttggttgctt gaattttgga aaacaaaagg tgcaaaagaa
    63601 gaatcgctgg caatgatata tttagcctta acaggaatag cttttaatcc tgcgagagta
    63661 gttgtagttg gcactgctga agatgatgaa gcagtccatt tcttgataat tttatcaaga
    63721 ctaccatctt ttttcatttc agacaaggct tggttaaatt cagtaactag gtgctcgtat
    63781 ttacttcctt ttttcacacc gaaagcaaaa cttcctacag cttcaccatc catttcaata
    63841 tggaggtctt gaccttggtt aatggcatat tcgataacag gtttgtcatc catcatggca
    63901 tcgatggcac cagcactcaa gctgttgttc attaaatcac cagtgtcaaa tgttttaata
    63961 gtaaagccgt atttatcttt gattgtttca aggaaacgtt gagcggcagt tccgttttta
    64021 acaccaacgg ttttgccagt taattggtcg tacttgctaa ttttgtgtga ctttgtagta
    64081 gcaatgacaa cttttgtatc atagtaagta tcagacatgg tgaagacttt ttcacgttct
    64141 ttagtctttg tcatccctgc catgatagcg tcggcttgcc cagcttgaac cgcattgact
    64201 gctgcgtcaa atccaggata ggacatctga atgttccagc ctttaatctc agcgactttg
    64261 ttaataatgt caacatcaat tcctttataa gtttgatctg aatctttaaa ctcaaaaggt
    64321 gcataggcgg tatcagaaac aatcttaatc gtttctgctt tggcaatacc taatgagaaa
    64381 attgggaata aaattagcaa aaatgctaga aattttttct tcatttttag tctccttttc
    64441 cgaatattct ctcattatat cagaaaaagt aaaaaaaggc aaatttttat atttttcgtg
    64501 tcagaaaata taacattgat ttttcctttt ctttcttgaa ttgctattaa aaagtgctat
    64561 aataatagta tatatagaag aaaagaggac ggggatatga agaacaaaag aatatttaaa
    64621 gacttccaag cttcaaaaat gagtttaaac atttacacaa gccccttgtt agcctttgtt
    64681 tttgtcttca taggagagtt tgtggctttt actttgtatg gtattggctt gttagctctc
    64741 atcggacttg ctagaaattt tggagaggct ggtcaaaatc ttgcaaccta cttgcagacc
    64801 ttgcatcaga gcttgacgga taaaacaagt gactttcgtt taattttagg attactggcc
    64861 tttggtttta ttcttaacac tgtgttcaga tggacaagaa aagttgagaa aagacctatt
    64921 cgaaccttgg gattttatag agagaatttc ctcagcaatc ttctgaaagg atttagtcta
    64981 ggcttggcac tttttcttct gaccttgtta ggtttagtgg tcttaggtca atatcgtttg
    65041 gaatccattc acttgaatcc ttattctctt gcctttgtcg tctttactat cccattttgg
    65101 attttacagg ggacagcaga agaagtggtg gcccgtgctt ggctacttcc tcaattggcc
    65161 tcaagaacca atctaaaact agctattctt atatctagcc tgttctttac cctgcttcat
    65221 atgggcaatt ctggcctcac ccctctatct ctagtaaatc tctttttatt cggagttgcc
    65281 atggctcttt atcttctcaa aactgataca gtttggggtg ttgcaggtat tcatggtgct
    65341 tggaattttg ctcagggtaa tctctttggg attttagtta gtggtcaacc gtcaggaacg
    65401 tctctgatga cctttttacc acaaggcaat caagattggc tatcaggtgg ttcttttggc
    65461 atagaaggtt ccattatgac aagtctggta ttactactgc tgattgtcta tcttgctaat
    65521 aaattaaaga aagaaaatga aaggatgtga cttcggtccg tccttttctt cgtgaaaata
    65581 ctataagtat gctaaaatag gaatagcaca tggagagagg attcttatga tcaatcacat
    65641 tacagataat caatttaaac tagtatcaaa atatcaacca tcaggagatc aaccccaagc
    65701 tatcgagcag ttggtggata acattgaggg gggagaaaaa gctcagattc tgatgggggc
    65761 gactggaaca gggaagacct atactatgag tcaggtcatt tctaaagtca ataaaccaac
    65821 tctggttgtt gcccacaata aaactctggc tggtcagctc tatggggagt ttaaggaatt
    65881 tttccctgaa aatgcagttg agtatttcgt atcctactat gattattacc agccagaggc
    65941 ctatgtccct tctagcgata cctatattga gaaggatagt tctgtcaatg acgagattga
    66001 caagcttcgc cactcagcta cctcagccct tttggagcgt aatgatgtta ttgtcgtggc
    66061 ctcagtctct tgtatctatg gtttgggttc gcccaaggaa tacgctgata gtgtcgttag
    66121 tctccgtcct ggtctagaga tttctcgtga taaactcttg aatgacttgg tcgatattca
    66181 gtttgaacgt aatgatattg atttccaacg cggaagattt cgcgttcgtg gggatgtggt
    66241 agagattttc ccagcttccc gagatgaaca tgcctttcga gtagaatttt ttggagacga
    66301 aattgaccgt attcgtgaag ttgaggctct gacaggtcag gtgttgggag aagtggatca
    66361 tttggcgatt ttcccagcta ctcactttgt gaccaatgac gaccatatgg aagttgccgt
    66421 tgccaagatt caagcagaat tagaagagca gttagctgtc tttgagaagg aaggtaaatt
    66481 gcttgaagcc cagcgcttga aacaacggac agagtacgat atcgaaatgc tgcgcgagat
    66541 gggctataca aatggggttg aaaattattc tcgccacatg gatggacgta gtgaaggtga
    66601 gcctccttat acgcttctcg acttcttccc agatgatttt ttgattatga ttgacgagag
    66661 tcatatgact atgggacaaa taaaaggcat gtacaatgga gaccgttcgc gtaaggaaat
    66721 gctagtaaat tatggtttcc gtttaccgtc tgccttggac aatcgtcctc tccgtcgtga
    66781 ggagtttgaa agccatgtgc atcagattgt ttacgtatca gcgacacctg gtgactatga
    66841 aaatgaacag actgagacgg tgattgagca aatcattcga ccgacagggc ttttggatcc
    66901 tgaggtggaa gtccgtccga ctatgggaca gattgatgat ctcttaggtg aaatcaatgc
    66961 ccgtgttgaa aagaatgaac gaacctttat caccaccttg accaagaaaa tggcagagga
    67021 cttgaccgac tatttcaagg aaatgggcat caaggtcaag tacatgcact cggatatcaa
    67081 gaccttggaa cggacggaga ttatccgtga cctgcgcttg ggtgtctttg atgtcttggt
    67141 cggaattaac ctgctccgtg aaggaattga cgttcctgaa gtgagcctcg tagctattct
    67201 cgatgctgac aaggaaggtt tccttcgcaa cgaacgtgga ctcatccaga ctattggacg
    67261 tgctgctcgt aacagtgaag gccatgtcat catgtatgca gacacggtta cccagtctat
    67321 gcaacgtgct atcgatgaaa ctgcccgccg tcggaaaatt cagatggcct ataatgaaga
    67381 gcatggtatc gtaccacaga caatcaagaa agaaatccgt gacctgattg ccgtgaccaa
    67441 ggcagttgct aaggaagagg acaaggaagt cgacatcaat agcctcaaca aacaagagcg
    67501 caaagaactc gtcaagaaac tagaaaaaca aatgcaagaa gccgtcgaag tgcttgactt
    67561 tgaactggcg gctcagatcc gtgatatgat gttggaagtc aaggccttgg attaggggaa
    67621 tagtatgatt tatttaagaa agttaaagag agaagatttg atgtctttat gggaaatggc
    67681 ttattcacaa cttaatccag tttggaaaca gtatgatgct ccctattatg atgattatca
    67741 gtatttttca aattttaaag aattcgaact acaaaaatca gaatccattt taagcaactc
    67801 aaatcgcctt ggtatttttg ttgatgataa actagttggg actgtttcgc gttattgggt
    67861 atgtaaacaa acaagatgga tggaattggg aattggtatt tatgataaaa aattctggaa
    67921 cactggtatt gggaaagttg ctatgttgca gtggatagat aggacgtttc aggattactt
    67981 ggagttggag catctgggtt tgacaacttg gtcagggaat cttggtatga tgaaacttgc
    68041 tgaaaaatta agaatgaaaa aagaagctca tattccaaaa gttcgttatt atcaaggtaa
    68101 atattttgat agtattaaat atggtatttt gagagaagac tgggagaaaa taaatgacgg
    68161 ttattatcaa atcaatggaa actcctgaag agatagaagg taaatccctc gttcactggc
    68221 aaacgtggag agaggcttat gatgaccttt tgcctgcaga atttcaggag acaatgacat
    68281 tagaaagatg tcgactcttt agtcaaaagt atccagaaaa tacattgatt gcgataggtg
    68341 atctgaaggt agttggtttt atcagttatg gtaactttcg tgatgagact attcaagctg
    68401 gtgaaattat tgctttatat gttttaaaag actattacgg aaaaggcata gcacaaaagt
    68461 taatgaaagc agctttgact gctcttaatc atttttctga aattttctta tgggtattga
    68521 aagataacaa gcgcgccatt gctttctatc aaaaaatggg ttttactttt gatggacaag
    68581 aaaaaatact tgaacttgga aagcctataa aggaaaaacg aatggtgttc tattctaaat
    68641 aattttaaaa aagtaaaaga taatatctct ggtagtaagt ctgaaaattt aataaattag
    68701 aaagcgagta aatttatgtc ccgttcccaa ttaacaattt taacaaatat ctgtctgatt
    68761 gaagacctcg aaactcagcg cgtggtgatg cagtatcgcg cccctgaaaa caatcgctgg
    68821 tctggttatg cctttcctgg aggtcatgta gaaaatgatg aggcttttgc ggagtctgtc
    68881 attcgtgaaa tctacgaaga aacagggttg actatccaaa atcctcaact tgtcggcatt
    68941 aaaaattggc cactagatac aggtgggcgc tatattgtca tttgttataa ggcgactgag
    69001 ttctctggta cccttcaatc ttcagaagag ggagaagttt cttgggtgca aaaagaccag
    69061 attccaaact taaatctggc ctatgatatg ctaccattga tggaaatgat ggaagctccc
    69121 gacaagtcag agtttttcta tcctcgccgt acagaagacg attgggaaaa gaaaatcttc
    69181 tagtctttta ctaaataacc tagctgatcc aaggcctcct cgatatagtg gaggtcttgt
    69241 tgtgtttcgg cttcgactag gtggtaatgg atgccatctg tcaattcaga tagaggtcta
    69301 aagtcagaat gctcgacttg ttctagaaaa tgttgcacat ctctgcgaca agacagttta
    69361 agcaaggttt caatttctcc ataaacggga tggtcaatca aggtattctg gacatgccca
    69421 ccattatcga caatagcaag gagttcctga ccgatttctt caacttcatg tttcactttg
    69481 aaaagtttgt gaacataagg attggtatca atttgcttat agatgtagcc acgattggtg
    69541 gataggatag gagcgccatc ggctctcaaa attgcaatgt cctgcacaat gacctgacgt
    69601 gtgacatgaa aatgttccgc caaactttgg ccattaaggg ctttaggagc ttctttcaag
    69661 agttggagca gggcttgttt gcgatctttt gtcatagctt ttccttttaa cggcgttttc
    69721 gaagcacttt atagacagct agtgctaatg tatagtctac catactatgg ataattgtgc
    69781 caaatccaac tagtacaaat agaacataga acatgttttc tacattggta cccgaagttg
    69841 cgtaaaaaag gacacaggct aatacttcag caagggcatg aacaacagcc aaaacaaaat
    69901 tgaaaaccca ggaagatttt ggtttatcta gggtatcggg gaatttttgt aggtaaagag
    69961 ctcctaaagc accaaaagat atatgggaaa aagcccgaaa aacgataacc atgggatagc
    70021 cagccatcaa aaatccaaaa ctagaggcta ggatgacaaa aactgccatc aagggcgaca
    70081 agaacatggc tataaaaata gcgatgtggc tccccaaagt ataggaagca ggtggaatga
    70141 caatcttgaa aggcataaca attggaatca aaatcgcaat agccgttaaa agggctgtca
    70201 ttgtcataaa ttgtgtcttt ttccgtgtat tcacaagaat ctccttttta actgcatata
    70261 cactagtatg gtacaataaa ccagacaata aagcaagaat ttacttgggt ttatagatca
    70321 ttttttagtt aaaagttata gtagattgaa actagaatag tccacctcta cttctaaaac
    70381 attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttcttatt tcgttttact
    70441 atagtaaaga tttcattaaa aagaaactgt atagagcaaa atctccacct tcaggtttga
    70501 aagcggagat tgtttttatt ttttcaaggt ttgtagtcgt ggaacaatgg ctagggttag
    70561 aattgcagaa atgaccaatt cagcaattga atttgttgag ataacggttg ccagaagaag
    70621 ttggatattt ccattataaa catttccaaa taggaagaag attcctccaa ggacaaagat
    70681 agtatttgtc aaggaaccaa gggctccagc taaaatcaga ccagtcttgt ttttcatcag
    70741 tttatagact aagtaaggag ttaaaccaat caaaatacgt gggacgatgg caatgatagc
    70801 tgagtagatg tttccgtttg gtacgaaggg agagaagagg tagcttgtcg gtagaatcgt
    70861 aatcgtgtta accgtcaagc taagtagccc catcaaaaat ccaagtgtaa ccccaactcg
    70921 tggaccataa ataatgctgg caataatgac aggaatatga acaatggtcg gtttgattgg
    70981 aaatggaaaa aggttaaaga taagtgagct cagaaagtgt atcacgagca tggtagcaaa
    71041 aaagatagca atgggtgcaa tattagagcg ttttttcatc gagagtttcc tttattcttt
    71101 ctaaaataat tgtgaggtca gctaaagctc ctcgtccgtg gtctccacaa gctagtaggg
    71161 attccttagg agcaatcagc tgatagccgt aggtttctaa tgttttcaga ttattctgag
    71221 ttactggatg gtcatacatt tttgtattca tagcaggagc tattagtttg ggaatatgac
    71281 ttggtagggc tagagctgta ctggttacca tgttgtccgc aaatccgtga gctagttttg
    71341 caatagtgtt agcagttgca ggtaccacga taaataaatc tgcttttttt ccaagttcga
    71401 tatgattgac ctgatcagga tagggttcct tcatgacatc caagtggaca ggattctgtg
    71461 agagtacctg tagtgtcaaa ggttggataa actctgtagc agcctgagtc attaagacag
    71521 tgacttgatg gccttgtttt tttagagaac tgactaaatc tgcagactta taagaggcga
    71581 ttgaacccgt tacagccaag agaatgtttg ccatagtttt cctttctaag aatgataggc
    71641 ttgaattttt tcaaggagga gttctgcaat ttcttcttta gtctggactg tttgaagctg
    71701 atttttctca acaaatatag ctcggtgctg atctgctgaa atttgagtca ggtcattcgc
    71761 gatgattaaa tctgcttgat tcttgataag actttttcgt gcaatgtcaa ccagatgatc
    71821 ttcggtaaca tcaaccagca gtttgaaacc aatcagatga atagtgggat tccattcctt
    71881 gactagggat atgattttgg gtgtcttttt aaggaacaaa acctgaacct catcagttga
    71941 agaaatcttg gcctgatgat tctgcttgct taaaaattct tttagattgg agctagcctg
    72001 aacttcctca agccctgtca tataaacagg agtgtagtca gaaacagcca ttgagtggat
    72061 caagacctga taatcctgaa cacgttcttg catttctatt agaaggtcct tggtattggt
    72121 aacttctcga atacttaggt taggatgagg ctctggcttc agagctcgtt ttgtcgtaat
    72181 taaacaaact tcatgccctg cagaaagcaa agtctctgtg ataattttcc ccaagtgacc
    72241 tgtagaatgg ttagtgatag agcggacgct atcgatagct tcactggtac cgcccgatgt
    72301 aactaaaatt ttcatagcac tattgtacac aaaaataagc ggtaagtaaa atgaaagtga
    72361 taaatttttg gtaaaaacga agatttacta tagtttcaaa ctataaagcc atataaaatt
    72421 taagaaagga ttctaaaagc cttaaaaaca gagatattta cgaacgttta gagggtttta
    72481 ggatgttaaa aatcttgttt tgtgatataa tgaattatca tataagaggt tagaggaatt
    72541 ttgaatgaaa acagatattg aaatcgcaca gagtattgag ttgaagccaa ttgttgatgt
    72601 tgtagagaaa cttggtattt cttacgacga tttggagttg tacggaaagt acaaggctaa
    72661 actcagcttt gataaaattc gtgcagttga gagcaatcca gtcggtaaat tgatcttggt
    72721 tactgccatc aacccaacac ctgcaggtga aggaaagtcg acgcttacca ttggtcttgc
    72781 ggatgccttg aacaagattg gcaagaaaac catgattgct atccgcgaac cgtctcttgg
    72841 tccagtcatg gggatcaagg gtggtgctgc tggtggtggg tatgcacaag ttctgccaat
    72901 ggaagacatc aacctccact ttactggaga tatgcatgct attacaactg ccaacaatgc
    72961 cctttctgcc ttgattgaca accacttgca ccaagggaat gagctgggaa ttgatcaacg
    73021 tcgtatcctc tggaaacgcg ttgtggactt gaacgaccgt gcgctccgcc atgtgactgt
    73081 tggtcttggt ggtcctctaa acggtattcc acgtgaggac ggttttgata ttacagttgc
    73141 ttcagaaatc atggcaattc tttgcttggc aacggacatc gaggacttga aacgtcgttt
    73201 ggcgaatatc gttattggtt atcgctatga ccgtacgcct gtttctgtag gtgatttgca
    73261 ggttgagggt gccttggctt tgattttgaa ggatgctatt aagccaaact tggttcagac
    73321 aatttacggt acacctgcct ttgtacacgg tggtccattt gccaatattg ctcatggctg
    73381 taactctgtt ttggcgacga caacagccct tcacttggct gattacactg ttactgaagc
    73441 tggttttggt gcggaccttg gtgctgagaa attccttgat atcaagacac caaacttgcc
    73501 aacatctcca gatgcagttg ttattgtcgc aacccttcgt gcccttaaga tgaatggtgg
    73561 tgtggctaaa gacgctctga ctgaagaaaa tgtagaggca gttcgtgcag gttttgctaa
    73621 cttgaaacgc cacgttgaaa atatccgtaa gttcggtatt ccagcagttg tagctattaa
    73681 cgaatttgta tctgatacag aagctgaaat tgcagtctta aaagaactct gtgcctcaat
    73741 cgatgtacca gttgaattgg ctagtgtctg ggctgatgga gcagaaggtg gagtagcact
    73801 tgccgaaaca gttgttaaga caattgctga aaatccagct aactataaac gtttgtatga
    73861 taatgacctt tctgtccaag aaaagattga aaaaattgtc actgaaatct atcgtggtag
    73921 caaagtgaac tttgagaaga aatctcaaac acaaattgct caaatcgttc aaaacggttg
    73981 ggacaaattg ccaatctgta tggctaaaac tcaatacagt ttctcagaca atccaaatgc
    74041 gcttggagca ccagaaaact ttgaaattac cattcgtgaa ttggtaccaa aattaggtgc
    74101 aggcttcatc gttgccttaa ctggtgatgt tatgaccatg ccaggtcttc caaaacgtcc
    74161 agcagctctc aacatggatg ttgaaagcga tggaactgta ctaggcttgt tctagtggat
    74221 tgaaattgac tttaagtgag tttggaaata atatccaagc tcattttttc tcagactgca
    74281 agcagttcat caataagatt tatactcttc gaaaatctct tcaaactacg tcagcttcac
    74341 cttgccgtag gtatatgata ctgactctgt cagttctatc cacaacctca aaacagtgtt
    74401 ttgagcaacc tgcggctagc ttcctagttt gctctttgat tttcattgag tataagtaga
    74461 tagaaaagtt atatactatc tattgaaaat attataaatc agatacaaag acaggtgaaa
    74521 ccttctgtgt tatactagag atatgttaga tttgaaagaa tacggtatcg tcatgtggcc
    74581 ggaggagaaa gtcatttctt tccgtgagaa acttctcgct tggtatgatg aaaacaaaag
    74641 agatttgcct tggaggagaa gtaaaaatcc ttatcacatc tgggtatctg aaatcatgct
    74701 tcagcagacc agggtggata cagttatccc ttactacgaa agattcctgg actggtttcc
    74761 aactgtcgaa agtctggcaa ctgcgcctga ggagagttta ctgaaagctt gggagggctt
    74821 gggctattat tctcgagttc gcaatatgca ggctgcagcc cagcagatta tgactgactt
    74881 tggtggtcaa tttccaaata cctatgaagg aatttccagc ttgaaaggga ttggacctta
    74941 cacagcagga gccatttcca gtattgcttt taacttgcct gagccagctg tagatggtaa
    75001 tgtcatgcgg gtcttggcgc gtctgtttga agtcaaccac gatattggga ttccaagtaa
    75061 tcgaaaaatt tttcaggcaa tgatggagat cttgattaat ccggatcgtc cgggtgattt
    75121 taatcaagcc ttgatggact tagggtctga tattgagtct cctgtaaatc ccagacctga
    75181 agaaagccca gtcaaggact ttagtgcggc atatcagaat ggaaccatgg accgttatcc
    75241 aattaaatct cctaagaaaa agccggttcc tatttatctt aaagcattag ttgtgaaaaa
    75301 ttctcaagga caatttttac ttgaaaaaaa tgaaagcgaa aagctcttgg caggtttttg
    75361 gcatttcccc tttatagaag ttgataactt ttcgcaagaa gagcagtttg acctctttca
    75421 tcaggttgcg gaagaaagtg tgaactttgg ccccagtcca gaagagagtt tccagcagga
    75481 ctatgaccta gatgttgatt ggcttgatgt ttgttttgat acagtccagc atgtttttag
    75541 tcatcgaaaa tggcatgtac agattgtagc aggtcaggtg agtgacttcc atgatttttc
    75601 agatagggaa gttcgttggc tttcaccaga agaattcaag aattacccac ttgccaaacc
    75661 ccaacaaaaa atctggcagg cttatgcaca agccaactta gacagtagcc aagactagct
    75721 attgtgtctt ctgactattt ttttggtata atagtagaga aaaaggtgaa catatgaaaa
    75781 aaatactaat tgtagatgat gagaaaccaa tctcggatat tatcaagttt aatatgacca
    75841 aggaaggtta cgaagttgta actgctttta atggtcgtga agcgctagag caatttgaag
    75901 cagagcaacc agatattatt attctggatt tgatgcttcc agaaattgat ggtttagaag
    75961 ttgctaagac cattcgtaag acaagcagtg tgcccattct tatgctttca gccaaagata
    76021 gtgaatttga taaggttatc ggtttggaac ttggggcaga tgactatgta acgaaaccct
    76081 tctccaatcg tgagttgcag gcgcgtgtta aagctcttct gcgtcgttct caacctatgc
    76141 cagtagatgg tcaggaagca gatagtaaac ctcaacctat ccaaattggg gatttagaaa
    76201 ttgttccaga cgcctacgtg gctaaaaaat atggcgaaga actagactta acccatcgtg
    76261 aatttgagct tttgtatcat ttagcatcgc atacaggtca agtcatcacg cgcgaacact
    76321 tgcttgagac tgtctggggt tatgactatt ttggtgatgt ccgtacagtt gatgtgactg
    76381 tacgacgtct gcgtgagaag attgaagata cgcccagccg accagagtat atcttgacgc
    76441 gccgtggtgt agggtattac atgagaaata atgcttgatt tactgaaaca aaccattttt
    76501 accagagatt ttatctttat cctgattttg ttaggtttca tccttgttgt gaccctctta
    76561 ttactggaaa atagacgtga taatattcag ttgaagcaaa tcaatcaaaa ggttaaagat
    76621 ttgattgcag gagattattc caaggttctt gatatgcaag gtgggtctga aatcaccaat
    76681 attaccaata atttgaatga cttgtcggag gttattcgtc tcactcagga aaatctagaa
    76741 caagagagta agaggctaaa tagtattctg ttttatatga cagatggggt tcttgcgacc
    76801 aaccgtcggg gtcagattat catgattaac gatacagcca agaagcaact ggggttggtt
    76861 aaggaagatg ttctgaatag aagcattttg gaattgctca agatagaaga aaactatgaa
    76921 ttgcgtgatt tgattaccca aagtccagaa ttgttgctag attcccaaga tatcaatggt
    76981 gaatatttga accttcgagt tcgctttgcc ttgatacgtc gagagtctgg ctttatttca
    77041 ggtttggtgg ctgttttgca tgatacgacg gagcaggaga aggaagaacg cgaacgaaga
    77101 ctctttgttt ccaatgttag ccatgagtta cggactcctc tgactagcgt aaaatcctat
    77161 cttgaagcct tggatgaggg ggctttgtgt gaaactgtag caccagactt tatcaaggtt
    77221 tctcttgatg agaccaaccg tatgatgcgc atggtgacgg atctcctcca tctttcacgt
    77281 attgataatg ctaccagtca cctagatgtg gaactgatta acttcactgc ttttattacc
    77341 tttatcctca atcgttttga caagatgaaa ggacaggaaa aggagaaaaa atatgagttg
    77401 gtgagagatt atcccatcaa ttctatctgg atggaaattg atacagataa gatgacgcag
    77461 gttgtcgaca atattttaaa taatgctatt aagtattcgc cagatggggg taaaatcact
    77521 gtcagaatga agacaactga agaccagatg attttatcca tttctgacca cggtttgggg
    77581 attcctaagc aggatttacc acgtatcttt gaccgtttct atcgtgtgga tcgtgctaga
    77641 agtcgtgcac aaggtggtac aggtctagga ctgtctatcg ctaaagaaat tatcaaacaa
    77701 cataagggct ttatttgggc caagagtgaa tacggcaagg gttcaacctt taccattgtg
    77761 ctcccttatg ataaggatgc agtgaaagaa gaagtatggg aggatgaagt agaagactag
    77821 aatgagtgaa ataggcttta aatacagtat tttagcgtcg ggttccagtg gaaattcttt
    77881 ttatctggaa acctcaaaaa agaagctttt agtagatgca ggcttgtctg gcaagaaaat
    77941 taccagtctg ctagctgaaa ttaaccgtaa gccagaagac ctggatgcca tcttgattac
    78001 ccatgagcat tcagatcata tccatggagt aggcgttttg gctcgcaagt atggtatgga
    78061 tctttatgcc aatgaaaaga cctggcaagc tatggaaaat agtaaatatc ttggcaaggt
    78121 ggattcttcg caaaagcata tttttgaaat gggtaaaacc aaaacctttg gagatatcga
    78181 catcgagagt tttggtgtaa gccatgatgc agtcgcaccg cagttctatc gctttatgaa
    78241 ggatgataag agttttgtcc tcttgacaga tacaggttat gtcagtgacc gtatggcggg
    78301 cattgtcgaa aatgcggatg gttatcttat cgaggccaac catgatgtag agattttgcg
    78361 atcaggttct tacgcttggc gcctcaaaca acgaatccta tctgaccttg gtcacctttc
    78421 taacgaggac ggtgctgaag ctatgattcg gacgctagga aatcgtacta agaagattta
    78481 ccttgggcat ttatctaagg aaaacaatat caaggaactg gctcatatga ccatggtcaa
    78541 tcagctggct caagctgatc tgggagtcgg agtagacttt aaggtttatg atacctcacc
    78601 agataccgca acaccattga cagagatata gaaagaacgc tgagaaggtg ttctttttat
    78661 attgactgga cacctaaaaa gtaatacaat ggtgttacca ttaaaaaagg gagcacaaaa
    78721 gatgactact attacattaa aagtttctga agctgataaa acatttatga aagcaatggc
    78781 taagtttgaa ggagtttccc tttcggaact tattcgcacc aaaactcttg aagccctaga
    78841 agatgaatac gatgctcgtg tggcagattt agcctatcaa gagtatttag aagacttgga
    78901 aaaaggagtt gaacccatta cttgggaaga aatgatgcat gatttaggct tgaaggatga
    78961 ataatttgta taaattagtt ccaacaagac gttttatcaa gcaattgaaa aaattggacc
    79021 gttatacgca gaagctaatt acaaactatt tacaaaccaa tgttttggaa gatccaagac
    79081 gacacggaaa ggctttggtt ggtaatcgcg ttggtcaatg gcgctataga attggtaatt
    79141 atcgagttat cgtacaaatt gtagatgatg aattagttgt tgctactcta gaagttggtc
    79201 atcggagaga tatttattga attaaagctg agtaataaca gtatagaaag taaggatagt
    79261 aaaaatttga ataaaaaatg gttatttgca gattattatg atacaacaat tattttactt
    79321 gctttaatct ctgttattct ggtattactt ggatttgctg agatgattga tttggacaat
    79381 ccgccttata gtattattga tttagtaatc tggggtgttt ttgtaattga ttatagttgg
    79441 cgctttttta ttactaaaag aaaatggcgc tttattcttg aaaatatttt tgatttattg
    79501 actattctcc ctttaaatgc tatttttaca gtgtttcgat taggacgtat cttccgctta
    79561 gcaaggctga caaaattact gaaactgact cgtttactta gaataattgg acttacaggt
    79621 aaattagaaa gaaaaattag tagattctta cggacaaatg gcctgattta tatcttatat
    79681 gttaatatct ttattgttct agtagggagt tcgattttat ccgttgttga agaaaaatcc
    79741 ttctcagata gtctttggtg ggctcttgtg acagtaacta ctgttggtta tggtgatatt
    79801 gttccagttt ccctttttgg gaaatggcta gcagttttac tgatgcttgt cggtattgga
    79861 acaataggga tgctaacaag tgctttgacg aacttttttg taaaggataa tccagatgaa
    79921 cagataaaac ttgataaact caaagacgaa ttatcgagcc aaagaatatt aatagagaaa
    79981 caatctgaaa aaatcgaaga actacatcga atgataaaag acttagttga aaaaatatca
    80041 taaaggtgag acaaaacaaa acttgattcg aggagaaatc aagtaagttt tgacacaata
    80101 gaacgcatat tatcaggtat aaaaacttga tactatgcgt tttattgtgg gaagatgtat
    80161 aatggattga aataagatat gaacaaatcg attaggaatt taaagcattt tataacaatg
    80221 ttttagagta acggggtact atttcatata atacaggaaa aaacaactcc ctgatgattc
    80281 aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg gatttttcca
    80341 tgcttcgtca ataatagctt gcaattcttt agcagatact tgcatttttt gagtttctgc
    80401 atcgttcaat ggaatgttta ctggacgaac gataccatgt gcaccaacaa cagctggttg
    80461 accgataaag acattctcaa ctccgtattg accttcttgg aatactgaaa gtggaagtac
    80521 tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg cgataccgta
    80581 gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac cttcgaacaa
    80641 ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt caaggtttac
    80701 accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt cacccatgat
    80761 gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt gacggaaacg
    80821 agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac cagagaattt
    80881 ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga tacctttgaa
    80941 accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt tacctacaag
    81001 gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtgatcacaa caaggtcagc
    81061 gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga aggcaagggc
    81121 gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa tttcgataat
    81181 tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg aacctacagc
    81241 accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg ttttaaacat
    81301 ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact tttaaaaaac
    81361 tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt agaggctgaa
    81421 atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga ggcatttatt
    81481 aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg caagttttat
    81541 cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag atggcttaaa
    81601 acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc cagacaaacc
    81661 ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc cacacgggga
    81721 ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc gttacatgct
    81781 tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg ctcaacgtta
    81841 taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca acaaaaatac
    81901 agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct tgccagcgcg
    81961 ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg caaccaatat
    82021 tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg ataatcctga
    82081 agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa ctggtgctct
    82141 tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt cgattgtcct
    82201 acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgtatcg ttgtaacaga
    82261 atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct tggttcagga
    82321 aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt
    82381 tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc tcttcaaaat
    82441 gacccaaatg caaaccaatt ttggtttcaa tatgctcgct atccaaaatg gtataccgaa
    82501 aattttgtct cttcgtcaga ttttagatgc ttatatcgag caccaaaaag aagtggttgt
    82561 tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct
    82621 cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga
    82681 tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc
    82741 tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga ttcaatctga
    82801 gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta agcctgaacg
    82861 tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt ctgataagcg
    82921 ccgtacagag ttgatgattg gacaggtctt gagtctcgag gatgaggact tgattgaaga
    82981 atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg atcaggacga
    83041 gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga aagatgatga
    83101 ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct tcacaaacaa
    83161 gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga ctgccaaagg
    83221 gctaccagta gtcaatctct tgaaattgga tgaagacgaa agtattcaga cggttatcaa
    83281 tgttgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc acggtattgt
    83341 gaagagaact agtgttaagg agtttgccaa tattcgtcaa aatggtctca aagcgctgaa
    83401 tttaaaggat gaagatgagt taatcaatgt cttgttggca gaaggagata tggatattat
    83461 cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc gtggtatgag
    83521 ccgtatcgcc actggtgtga aaggggttaa ccttcgtgaa ggagacacag ttgttggtgc
    83581 cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat atggtaagcg
    83641 tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc agacagctaa
    83701 aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaagggg atgaggattt
    83761 gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata tttcacaaac
    83821 aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc agatagtgac
    83881 tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg aaacagaagg
    83941 tgaagcataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca
    84001 aatatcctag caggatttct gatattactg tcactggctt tgatttttaa tactcaaatt
    84061 cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt atcaaaagaa
    84121 aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt caaagctatc
    84181 tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt tattggggga
    84241 attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga taatgttaat
    84301 ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg aaattatagt
    84361 ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt ttctccttta
    84421 gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt ttatacttat
    84481 gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga tgatagagat
    84541 ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga acgtattatt
    84601 gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctgatga aatcctaaca
    84661 gctttcaatc aaccatataa acaattttat taatacaaat cagtgaaatc atggatttca
    84721 ctgatttttt aagattttca taaaaatata gtaaaatgaa ataagaatag gacgaatcgt
    84781 tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt
    84841 tcaatatact ataaactttt atgaaataca gaaaacgctt cctaaaacct aaagtttgtg
    84901 atataattat caagaaaaaa gatttaggag tttattatga tttcttcaga atttatctca
    84961 aagattgaat ttgcttgcaa taagaaagaa agtctttata gtcaaagcaa atttaagtat
    85021 gcgattcgtt cgatgttcgc aggtgcattt ttaaccttca gtactgctgc aggtgcagtt
    85081 ggggctgact tgattaataa aattgcacca ggtagtggac gcttcctctt tccattcgtt
    85141 tttgcttggg gcttggccta cattgttttt ttgaatgccg agttggtcac ttcaaacatg
    85201 atgttcttga ctgctggtag tttcttaaaa aaaatctctt ggagaaaaac agctgagatt
    85261 ttactatact gtaccttgtt caaccttatc ggagccttga tagcagggtg gggctttgct
    85321 cattcggcag cctatgcgaa tctgacacac gatagtttca tctcaggtgt tgttgagatg
    85381 aagttaggcc gctcaaatga attggtcttg cttgaggcga ttttggcaaa tattttcgta
    85441 aatattgcga ttctgtcatt tattttggtc aaagatggtg gtgtcaaact ttggcttgtg
    85501 ttgtcagcta tttacatgtt tgtattctta acaaacgagc acattgcggc gaactttgct
    85561 tctttcgcga ttgtgaaatt cagtgttgct gcgaattcaa ttgccaactt cggtgttgga
    85621 aatatgcttc gccactgggg tgtgactttc atcggaaact ttatcggagg aggcctcttg
    85681 atgggtcttc catatgcctt cctcaataaa aacgaagata cttatgtaga ttaagaaaat
    85741 gagcacgatt gagtcgtgct tttttcattt tcaaaataag gtaatagcta tttcttatat
    85801 caaaatatag aaaactgata tttgtaaact ataactcaag gtgctacaat atccttaata
    85861 aaataatatg gaggtcacct tatgactcgt gattttaaat ttgaaactct acaactacat
    85921 gctggtcaag ttgtggctcc agctactaag tctcgtgcag tgccgattta tcaaacaaca
    85981 ttttttgttt ttgatgacac gtaggaaggt gccgatctgt ttgccttgag gaaaccaggg
    86041 aacatttata ctcgtatcac caatcctaca acagctgccc ttgaaggtgg tgttgaagcg
    86101 ctagcaacag catcaggtat gactgcagtg acttatacga ttttggcgct tgcccatgct
    86161 ggtgagcatg tagtggctgc ttcgactatt tacggtggaa ccttcaatct tttgaaagaa
    86221 ccccttcctc gttatggtat cacaacaacc tttgtcgatg ttgataattt ggaggaagta
    86281 gaagcagcta tcaaagacaa taccaagctt gtcttgattg aaaccttggg taaccccttg
    86341 attaatattc cagacctgga aaaactggca gagattgctc ataaacatca aatcccactt
    86401 gtgtcagaca atacttttgc aacaccttat ttgattaacg tcttctctca tggcgttgac
    86461 attgccattc actctgcgac taagtttatc ggtgggcatg gtacaactat tggaggaata
    86521 attgtcgata gtggtcgttt tgactggacg gcttcaggga aattccctca atttgttgac
    86581 gagagtccaa gctgccacaa tttgagctat actcatgatg tgggtgcagc agcctttatt
    86641 atagctgttc gagttcaatt gcttcgtgat acaggtgcag ccttgtcacc attcaatgct
    86701 ttcctcttgc tacaaagact tgaaaccctt tcacttcgtg tggaacgcca tgtacaaaat
    86761 gctgagacaa ttgttgattt tcttgtcaac catcctaagg tagaaaaggt aaattatcca
    86821 aaacttgcag atagtcctta ttatgccttg gctgagaaat acttgccaaa aggtgtcggt
    86881 tcaatcttta ccttccacgt caaaggtgac gaggaggaag cacgcaaggt cattgataat
    86941 ttagaaatct tttctgacct tgcaaacgcg gcagatgcta aatcgcttgt tgtccatcca
    87001 gcaacaatca ctcacggtca attgtcagaa aaagacctag aagcagcagg tgtcacacca
    87061 aatcaaatcc acttgtctat cggtcttgaa aatgtagaag atttgattga agacttacgc
    87121 ttggccttgg aaaaaattta aagtaaaaga agataaacag tgggttttga ctcactgttt
    87181 ttgattttcc ctcaggcatg atataatggt tacagaagtc tagaaagagg aatgtaatga
    87241 acgaaatcaa atgtcccaac tgtggggaag tctttacagt aaatgaaagc cagtatgctg
    87301 aacttttgtc ccaagtgaga acggcagagt ttgataagga actacacgat aggatgaggc
    87361 aggaactggc cttggctgag caaaaggcta tgaatgagca acagactaaa ctggctcaga
    87421 aggatcaaga aattgcgcaa ttgcagagtc aaatccaaaa ctttgataca gaaaaagaat
    87481 tggccaagaa agaagttgaa cagacaagcc atcaggcctt attggctaag gacaaggaag
    87541 tacaggcctt ggaaaaccag ttggcgacct tgcgtttgga gcatgaaaat caattgcaaa
    87601 agaccctttc tgacctagaa aaagaacgca atcaggtcaa aaatcagctc ctactgcaag
    87661 aaaaggaaaa tgagttgtct ttggcttctg ttaagcaaaa ctacgaagcc cagctcaaag
    87721 cagccagtga acaagtcgag ttttataaga attttaaggc tcaacaatct acaaaagcga
    87781 ttggggaaag cctagaacag tatgcagaga gtgagtttaa caaggttcgt agtttcgcct
    87841 ttccaaatgc ttactttgag aaggataaca aggtctcttc gcgtgggtct aaaggggact
    87901 ttatcttccg tgagtgtgat gaaaatggag ttgaaatcat ttctatcatg tttgagatga
    87961 aaaacgaagc ggacggaaca gagaagaagc acaagaatgc agatttttac aaggaattgg
    88021 acaaggaccg tcgggagaag aactgtgagt atgccgtttt ggtgaccatg cttgaggctg
    88081 ataatgacta ctttaacaca gggattgttg acgtcagtca cgagtatgaa aaaatgtatg
    88141 ttgttcgtcc tcaattcttt atccaattga ttggtctctt acgtaatgcg gcgctaaatt
    88201 ccctaaaata caagcaggag ttggccttgg ttcgcgagca aaatattgac attacgcatt
    88261 ttgaggaaga cttggatgcc tttaagctag cttttgctaa gaactataat tcagcttcga
    88321 ctaactttgg aaaagctatt gatgaaatcg acaaggccat caaacgcatg gaagaggtta
    88381 agaaattcct gactacatct gaaaaccaac tccgtctagc taacaacaaa ttggaagatg
    88441 tctctgttaa aaaattgacc cggaaaaatc caacaatgaa agcgaagttc gaagcattga
    88501 agggggagtg aggaaataag ttatggaaga tgatcttaat tatgaaaatt tgatggatga
    88561 tgtaaccgag gctataaaaa agtttaactt agtaattttt attggcgcag gagtttcaat
    88621 tgctcaaggt tatcctaatt ggaacaatta tattgaacat cttattaagt attggcaagg
    88681 acaagtttta tcggtaagtg gtgaaaaaag gcttggaaga gaacatcacg tagtttttga
    88741 tttaattagc aaatctagta ttagtaataa acgtaaagtg gatcttgtaa actatgaatt
    88801 aaaaaaagtt tttggtgaag attttgaaaa gcgacgacta gattttgaaa aaggatattt
    88861 taaaaatcta ttaccatatt caatagttaa tcaaacagta gaatctttag caagtttgaa
    88921 tgcaatattt attacttcta actacgacta tgaaattgaa aatcatatta aacgtttaaa
    88981 gaatgctgta gttactatta atgatctcaa tgaattcact aagaacaaga atgggaaact
    89041 acaatttggt gatgttctac atatccatgg aacaccagat tgtgatgtaa agtattttgt
    89101 aagttcatcc gcagattatt caaaaaccta tttaaaaaat agggaaaatt ttgaaaattt
    89161 agtaacatgg tttaaagaga caaaacctac tgttttattt gttggagcag gtcttgaaga
    89221 agatgaaatt ttatctttac tttgtaaaga tagtaagaac tatgccttga tgaagtcaga
    89281 gaacacaggt aatcagagag ttgatgaaca ttatagaggt gttgttgaag gattttttag
    89341 ctcagaaaat catactcaga ttatttggta cggagatgaa tttgagaaac tacctctttt
    89401 tgtaaaaaaa ttagttgctg atattaatga aaaattgggc acacatgatt tttataatca
    89461 gtggaacaat cttttgaatc cttctataaa tcaagaagag tataataaaa atctagatag
    89521 tatttctaat gattttaagt atctaagctc tgttttagat aaggttattg aaaatgataa
    89581 taatcaacta gatcagctaa tgttaaatgc tctgcttcgg agtgaaactc taacagtaat
    89641 taaaaaaaat tttgtccttg cattttggag gtttattgtt aagaatattg aaaagttaag
    89701 tgataatgag tgggatgtga tttataaaat aatttatgaa ggctctcaaa attactttat
    89761 agatgatgta ttctttgtct ataattatgc gatagataat aaaatttcta gttttacaaa
    89821 taataataag cttaatgaac tgagagaaat tatatccaaa gatggatata tcgttaattc
    89881 taactttaat aaagataaaa ctcttttggg ttactggcta gtaagtgctt ttgaacagca
    89941 aaatcgtgat ttatatataa aggaagatag tgaagtagaa gttaacttaa attacgaatg
    90001 tgttaacaaa ttaatgtcca tactaaataa tccagaattt ttaagttata attactactc
    90061 tatagagcat caattaaaag agtatgatgt tgttaaattt ctttatgagc ttgtaaagtc
    90121 caaaaaatta tttattgaag aggaaaagtt tttagaaagt gattctgaag acttaataag
    90181 tactatatta attcaaaaat tgcttgttca attggataat gaaattaatt tggatttaga
    90241 atttatcaag aggttaattg ataaaattga tttttcaaat attcactttg gagaggaatt
    90301 aaacactttt atcaaagaac atagaagtat tattagagaa aaaaatattg aaataccaaa
    90361 aaaaccttat agaaattgga tatcttcact agaaggaggt tttgtttcac aattttcata
    90421 tttaactcaa gaaaatcttg tagagtacga tgaatctaga gtattagaga tattagtcaa
    90481 cgccgaaaag gaacaaagag gttcttcatt tttggaagaa aagacgataa atgaaacgga
    90541 aaatttcttt attactgttt taaaagaatc aaatgaaata tctaaaaaag tttctgattt
    90601 attgaaaaat catatagatg acttatatcc aaaatataag agattatatg taaaaattat
    90661 cagttttcct gaaatagaag aaaatttgag aaagattgtt agagaaaaat atttaaaaag
    90721 atttaacaaa gaaagttttg attctaacga tcggaaattc tttgaatatc atataaaaca
    90781 acaaaatacg gatatagata tttttgaaaa attacttagt atcaatgtga atgaactctc
    90841 aacaccaaaa ggtgataata aacaattaga tatacttcat tttataaata gtgaaatggg
    90901 atcatatttt caatgtttaa tatcattatt tataaatcat tcaagttata gagatgtaat
    90961 tattcaaata ataaattcag taacagatac cgattatagg gagtttgctc aaggtatttt
    91021 attaaatgaa tacaatccaa acagaatcaa tgtaacttac aacacctttt taggatttgc
    91081 gtattatcat tctacaatta ctatcgaagc tgcggatgtt ttcacagatg tagtaaggga
    91141 tattctaaac aaaaaaattg aggacaatca aattcttaat aaagtttatc tagttgcttt
    91201 agagagggta gatccaacta ttgaatcatt ttctttatca aagaataact atagtcaaat
    91261 gataaacatc atttttacag gagactatga atttagatat tctaaagaat ggttgggagc
    91321 attattcaaa tttgactcaa gtgcaaatta tttggtgaca attttttatt tattatataa
    91381 tgaaaatctt aaaaaaaata gatttgcttt gtttatagag gaattgagtg attacttaac
    91441 aacttataac cagaaattat ctttacgagg gatgaattat aaattgaatc atgaagaact
    91501 taacaatttt gatttgttga aaaaaatgtt tctgaaatta atggagaccg ataaaataga
    91561 gaatgatatt ttttatttag atggcatcaa aagtatactc ccattattat ctttagatga
    91621 cagacgaaat gtattacaac atattcaaaa acagaacaat tgtcctcctc cagagattga
    91681 ggaattacaa agaattatag ttaattaaaa tttgtaaaaa agagaataaa aggtagacga
    91741 cgcctcagta aaaattggcc cggaaaaaag cgaagttcga agcactgaag ggggagtaga
    91801 aagcaaaaat gaacggtatt atcaacttaa aaaaagaagc aggaatgacc tcgcatgatg
    91861 cggtttttaa actgcgtaag attttgggaa ccaagaaaat tggtcatggt ggaaccttgg
    91921 atccggatgt ggtgggtgtt ttgccgattg cggttggcaa ggcgacacgc atggtcgagt
    91981 ttatgcagga cgagggtaag atctatgagg gggaaatcat tctgggctat tccacgacga
    92041 ctgaggatgc tagtggggaa gtggtcgcaa aaacccctgt tttgtcttca ttggatgaaa
    92101 agcttgttga tgaagcgatt gctagcttga ctgggcctat tactcagatt ccccctatgt
    92161 attcggcagt taaggttaat ggtcgcaagc tctatgagta tgcgcgtgct ggtcaggaag
    92221 tggagcgtcc agaacgtcag gtgatcattt atcaatttga gcgaacaagt ccgatttctt
    92281 atgatggcca acttgcccga ttcacttttc gtgtaaaatg cagtaaaggg acttacatcc
    92341 gtactttgtc agttgatttg ggtgaaaagc ttggttatgc ggctcatatg tcccatttga
    92401 ctcgtactag tgctgctggc ttacaattag aagacgctct tgccttggag gaaattgctg
    92461 aaaaagtaga ggctgggcaa ttagattttc tccatccttt agagattggg acaggtgatc
    92521 ttgtcaaagt tttcctaact ccagaagagg ctacagaagt tcgctttggt cgttttattg
    92581 agctagacca aacggacaaa gaactggctg cctttgaaga tgataaattg ttagccattc
    92641 tagaaaaacg gggcaatctc tataagccaa ggaaggtttt tagctagatc gtttaggaat
    92701 aaaaatcggg tgatagataa caattgcttg ataaaacccc atactaatag tagaatggtt
    92761 ttgggaatta taatattcca attgttgcga gttgtaggta ctcaaataat ctatatagaa
    92821 atttagaggt gtgaaatgaa gcaatttaaa attctttcag ataaatattt agagtccatt
    92881 acaggttctg gtgggaactt aggcccagga tttggtgtga taattccatg atgcgaaatg
    92941 agtttcgaga aagggtggag caacttcttc aacaaaaaga aataaatgaa aatagtgagt
    93001 tgagtcacct gtttcgtctt gctatacaaa atttagacag aaatgaaaaa taccaaacgg
    93061 tcatggccaa tttgagtcaa gggttgtcac tttacctcat gacgcatcat taccaggcac
    93121 ctaagtctgt cattgatttt ggtttatgga ttgccaaagc tcctagccag gaaagaggga
    93181 gactggcttt cttgcagatg cttgctcaaa ccctacaggg ctttcgttaa aactgtcgaa
    93241 catcagaggg aagactctgg tgttttttgc tgatgagact ataaattttg taaaaaatgt
    93301 gatagaatag acgacggata aaataacgga ggatagcatg caaaatagac caatcattat
    93361 tggagtgaca ggtggttctg gtggtggtaa aaccagtgtt tcaagagcca ttttatcgca
    93421 ttttcctgat gaaaagattt ccatgattga gcatgattca tactacaagg atcagtctca
    93481 tttgaccttt gaagagcgtg tcaaaaccaa ctatgaccat ccttttgcct ttgatacaga
    93541 cttgatgatc gagcagatta aggaattgtt ggcagggcgt ccggtggaca tcccgaccta
    93601 cgactataca gagcatacac ggagtagcaa gacctatcgt caggagcctc aagatgtctt
    93661 tatcgttgag ggcattttgg tcttggagga caagcgtctg cgcgatttga tggatatcaa
    93721 gatttttgtg gatacggatg acgatgtgcg tattattcgt cgtatcaagc gtgatatgga
    93781 ggagcgtggc cgtagccttg atagcgttat taaccagtac ttaggtgtgg tcaaaccaat
    93841 gtaccaccag tttatcgagt caactaagcg ttatgctgat atcgtcattc ctgaaggggt
    93901 tagcaatacc gtggctatcg acctgttgac gaccaagatt gcaaagattt tggaagaagc
    93961 tcgaaacagc aaataatcac atgtggaggc cttgcctcct ttttctattt ttccctcata
    94021 atggtacata aatggagatt tttaggcata tttttggtat aataataccc atgaaagagc
    94081 aagtaaatga atagtaggtg gagatggaaa agtatttatc ggtaacaact ttgaccaagt
    94141 atctgaaaat gaaattcgat aaagacccat acttggaacg ggtctattta actggtcaag
    94201 tttccaactt tcgtaaacga cctactcacc aatatttctc cctaaaggat gaccatgcag
    94261 ttattcaagc gaccatctgg tctgggattt atcagaaatt agggtttgac ctggaagaag
    94321 gaatgaagat caatgtgatt gggcgtgtac aggtctatga accaagtggt agctactcca
    94381 tcatcattga aaaagttgag cctgatgggg ttggggcgct tgcgattcag tttgaacaac
    94441 ttaagaaaaa attgacagaa gaaggcctgt ttcaagaacg cttcaagcaa gctctgcccc
    94501 aattttctaa gagaattggg gtagtaacca gccgtagtgg agccgttatt cgagatatta
    94561 tcacgaccgt cagcaggcga ttcccaggtg ttgacatcct tctttatccg accaaggttc
    94621 aaggtgaagg ggctgcggag gaaattgcta gaaatattgc gcgtgctaat caacgggacg
    94681 atttggattt gctcattatt ggtcgtggag gtggttctat tgaggatctc tgggccttta
    94741 acgaagaaat tgtggtacga gctatttttg aatctcgttt gccagttatc tctagtgtgg
    94801 ggcatgagac ggatgtgacc ttggcagatt ttgtggcaga tcgacgcgct gcaacgccaa
    94861 cagcggcggc tgaactggca acacctgtga ccaagttgga tgtattagct catttgcaaa
    94921 atcaggaaaa acggatggta acggcagtcc gaaatgttct atctaagaaa caagaggctt
    94981 tgaaaaaatg cagtcagtct gttatcttta gacaacctga gcgcttgtat gacggttatt
    95041 tgcaacgctt ggaccaactg caactgcgtt tgaaacaaag tttgcgaact cggatttctg
    95101 ataacaaaca attagttcaa gcaagaactc atcaattagt acaattatca cctgttacca
    95161 aaatccaacg ctatcaagac cgtttaggac agttggacaa gctcttaggt agccaaatgg
    95221 cgttagttta tgacgccaag gttgctgagg tcaagcgact ttcggaagct ttgctcatgt
    95281 tggatactag ccgaatcgtg gcgcgtggtt atgctattgt caaaaaagaa gagtccgttg
    95341 tagattcggt tgagagtttg aagaaaaaag accaagtaac gcttttgatg cgagatggtc
    95401 aagtagaatt agaggttaaa gatgtcaaaa caaaagaaat ttgaggaaaa tctagcagaa
    95461 ctggaaacca ttgtccaaag tttggaaaat ggtgaaattg ctctggaaga tgcgattact
    95521 gcctttcaaa agggcatggt cttgtcaaaa gagctccaag ctacgctgga caaggctgaa
    95581 aagaccttgg tcaaggtcat gcaagaagac ggaacagaaa gtgattttga atgaaaaagc
    95641 aagaaaaatt agctcttgtc gagtcggctt tggaagattt ttatggagac cagcagtttg
    95701 cctctagttt acgggagtct gttctctatt ctattcatgc tggtggcaag cgtattcggc
    95761 cttttctctt gttagaagtt ctggaagcct tgcaggttac catcaaacct gctcacgcgc
    95821 aggtagctac tgccttggag atgattcata cagggagctt gattcacgat gaccttcctg
    95881 ctatggatga tgacgattat cgaagagggc ggttaaccaa tcacaagaaa ttcggtgaag
    95941 ctatggccat tttggctgga gatgccttat tcttagactc atatgccttg attgcgcagg
    96001 cagatttgcc aagtcagatt aaggtggact tgattgccaa cttatccctt gcttcaggta
    96061 gtctgggtat ggtggcaggg caagttttgg atatggaggg cgaacaccag cacttgtctt
    96121 tggaagaact tcagactatt catgccaata agactgggaa gttactagcc tatcccttcc
    96181 aagcggcagc tattatagct gaattgtcac ctgaaatgca ggtgaagctg aaaactgtgg
    96241 gtgaattgat tggacttgct tttcaagtca gagatgatgt actggatgtg acagctagtt
    96301 ttgaggaaat cggcaagaca cctcaaaagg atctgcaggc agaaaaatca acctatcctg
    96361 ccttgttggg cttggaagag tccattgcct tttgtaacca aaccctggat caagctaatg
    96421 aaaaattaga agaaattgcc cagcagcttc cctttgaaac agaatcgatt gtaagtgtag
    96481 tagaaagttt gagaatcaat ggctaaggaa agagtggatg tactagctta taaacagggg
    96541 ttgtttgaaa cgagagagca ggccaagcga ggtgtgatgg ctggtctagt cgtagcagtc
    96601 cttaatggag aacggtttga caagccagga gagaaaattc cagatgacac cgaattaaaa
    96661 ctcaaggggg agaaactcaa gtatgtcagc cgtggtggtt tgaaactgga aaaggccttg
    96721 caggtctttg atttgtcggt ggatggcgcg actacgattg atatcggggc ctctactgga
    96781 ggttttaccg atgtcatgct acagaatagt gccaagttgg tctttgcagt cgatgttggt
    96841 accaatcagt tggcttggaa attacgccaa gacccacgag ttgtcagcat ggagcagttc
    96901 aattttcgct atgctgaaaa gactgatttc gagcaggagc cgagctttgc cagtattgat
    96961 gtgagtttca tttcccttag tctgattttg ccagccttgc accgtgtctt ggctgatcaa
    97021 ggtcaggtgg tagcacttgt caaacctcag tttgaggcag gacgtgagca gattgggaaa
    97081 aatggaatta ttcgagatgc taagattcat cagaatgtcc ttgaatctgt aacagctatg
    97141 gcagtagagg caggtttttc agtccttggc ttggactttt ctcccatcca aggtggacat
    97201 ggaaatattg aatttttagc gtatttgaaa aaagaaaagt cagcaagcaa tcagattctt
    97261 gctgagatta aagaagcagt agagagggcg catagtcaat ttaaaaatga ataaaaaaga
    97321 gagacttgaa aaaattagaa gattggtgac agattatcaa atcggcacgc aagaagaaat
    97381 tgtagaacat ttgaaagaag caggtatcac tgccactcag gcgacggtat cccgagatat
    97441 caaagagtta ggtattgtca aaattccttt gagagacaac acctatgtct acgaattgcc
    97501 aaaatcaatc gtaaaaagtc tgcaactggc tgaagacaat atcgaatcgg ctgaattgat
    97561 ggataagatg atcaatctcc aagttattcc aggaaatacg gcttttgtaa aagctcagtt
    97621 aatcgagact tttgcagaca agatttttag ctgtttgact gatgatagct cgattttagt
    97681 cattgccaga agtggaagtc tagcagagga aatctttgaa caagtaaaaa attggtaggt
    97741 ctatatgtta cttgaaattt cgataaaaaa ctttgccatt attgaggcta tttccctcaa
    97801 ttttgaaaag gggatgactg tcctgactgg tgaaacgggt gcagggaagt cgattatcat
    97861 tgatgccatg aatatgatgt tgggagctcg tgcgacgaca gatgttattc gtcatggtgc
    97921 accaaaggca gagattgagg ggcttttctc agttgagaat agtcgtcttt tacaggaaat
    97981 ttttgatgag caaggtttag aattaggtga tgaaattatc atccgtcggg aaatcttgca
    98041 aaatggtcgt agtatcagcc gtgtaaatgg tcagatggtt aatctgtctg ttttgcgagc
    98101 tattgggcaa catcttgtag atattcatgg tcagcatgac catgaggagt taatgcgtcc
    98161 ccaactgcat atccagatgt tggatgaatt tggtgatgcc gctttttggg acttgaaaga
    98221 aacctatcaa acgagttttg atgcctatcg gaaaatgcgc aagcaggttc tggaagtcaa
    98281 gaaaaaccaa caggaacaca aggcacgtat cgaaatgttg gaatttcaaa tggcagagat
    98341 tgaggcagca aacttgcagg ctggagaaga cttggctctc aatcaagagc gagataaact
    98401 cctcaaccac aaaaatattg cggatacact gaccaatgcc tacagtatgt tggacaatga
    98461 agatttttca agtctggcca atgttcgttc agctatgaat gacatggaaa gtgtcgaaga
    98521 gtatgatcct gaataccgtg aaatttcaag ctctctgtct gagacctact gtgttttaga
    98581 agacattagc aaacgtttgg aagctatcat tgaggacctt gattttgatg gcaatcgcct
    98641 catgcaggtt gagaatcgtt tggacctcct tcatactatt actcgtaagt atggtgggac
    98701 tgttgatgat gttttgcttt attttgccaa gattacggaa gaatacaatc tcttgacagg
    98761 caataatctt tcgtctgagg acatggaagc agagcttaag aagttggaag tcaatcttgt
    98821 caatttggca ggtcaacttg cttctgctcg tcataatttg gctcagcaac tcgaagctga
    98881 gattaaacaa gaactgcaag atctttatat ggaaaaagcc cagtttcagg ttcgttttag
    98941 taagggaaaa ttcagtcgtg agggaaatga aatggttgag ttttatattt caaccaaccc
    99001 tggagaagac tttaaaccct tggttaaggt tgcttctgga ggggaattat ctcgtctcat
    99061 gttagccatt aagtctgcct tttcacgtaa agaaggcaag actagcattg tctttgatga
    99121 agtggatacg ggagtttcag gtcgtgttgc tcaagctatt gctcagaaaa ttcataaaat
    99181 tggtcagcat ggtcaggttt tggctatctc ccatttgcca caagtaattg cgattgcaga
    99241 ttatcaattc tttattgaga agattagtaa tgaccattca acggtttcga ctgttcgtct
    99301 cttgacggtc gaagagcgag tggaggaagt tgccaagatg ttggcaggtg atgatgtgac
    99361 agaagcagcc ctgacgcaag ccagagaatt gttgagaaac agggagaaat aagatgacag
    99421 attattatgt aattggagat gttcacggaa aagctgggat gctggaagac cttcttaaaa
    99481 cctgggatgg tcagacccag ttgctctttc taggtgattt gattgacaga ggtgaggata
    99541 gtcaccgtgt tcttgaaatg gtcaaggact tggtggacaa tcaaggggct atctgtttgt
    99601 ctggaaacca cgagtatatg tttttgactt ggctagatga cccagaagaa agttatgacc
    99661 attatcgtcg caatggtgga gatacaacca ttaactctat cctaggtcgt cccttggatg
    99721 caccagttga tggagtagaa gatgccaagc gggttgccgc tgaagcggca gacttggtcg
    99781 aattcattcg tcaaatgcca tttgtagtag agacagacaa gtatatcttt gttcacgcag
    99841 gtattgattt gaccttggat gactggcatg aaacaacaga ttataaaaaa gtatggctta
    99901 gaaaaccatt ccacggagct gaaaatcata ctggaaaaac cattgtcttc ggtcatacac
    99961 cggtctatgg tttgttacag caagaacgcg gtacatctga gctttggact acagaagatg
   100021 gcaagattgg aatggatgga ggagctgttt atggtggtgt ccttcacggg attgtcttta
   100081 cagaccaagg aatgacagaa caccacttta tcgaaaatga cggctttgtc gctgaagatt
   100141 agtactccta gcagggtatg gtcttgtcaa aatatcaaaa acaatttata ataaatagat
   100201 accctgaaag gaagagaatc atgaacttag aagaattgaa aaaacgacag gagaagatcc
   100261 gtaacttctc tattatcgcc catattgacc atggaaaatc aactctagca gaccgcattt
   100321 tggaaaaaac agagacggtt tcaagtcgtg aaatgcaggc ccagcttttg gatagcatgg
   100381 agctagagcg ggaacgtgga attactatta agttgaatgc catcgagttg aattacactg
   100441 caaaagatgg ggaaacttat attttccatt tgattgacac accagggcac gttgacttta
   100501 cctatgaagt ttcacgttcg ctagctgcct gtgagggtgc tattttggtg gtcgatgcgg
   100561 ctcaaggaat tgaggctcaa actcttgcca acgtttatct ggctttggat aatgatttgg
   100621 aaatcatgcc tatcattaat aaaattgacc tgccggctgc agatccggag cgcgtgcgta
   100681 cagagattga agatgtgatt ggtttggatg ccagtgaagc agttttggct tctgccaagg
   100741 ctggtattgg gattgaagaa atcctcgagc aaattgtaga aaaagtacca gcaccaacgg
   100801 gtgatgtgac ggcgccactt aaggccttga ttttcgactc tgtttacgat gcttaccgtg
   100861 gggttatcct ccaagtgcgt gtcatggacg gagtggtcaa acctggtgat aagattcagc
   100921 tcatgagcaa tagtaagacc tttgatgtgg ccgaagtcgg tatttttaca ccaaaagcgg
   100981 ttggtcgtga tttccttgcg actggtgatg ttggttacat tgcggcgtct atcaagacgg
   101041 ttcaggatac tcgtgtgggt gataccgtta ccttggcaac caatcctgcg gcagaaccat
   101101 tacatggtta taagcagatg aatcctatgg tctttgcggg tctctaccca atcgaatcaa
   101161 acaaatacaa tgacctgcgt gaagcgcttg aaaaattgca actgaatgat gctagtcttc
   101221 agtttgaacc agaaacatct caggcacttg gatttggttt ccgttgtgga tttcttggac
   101281 ttctccatat ggatgttatc caggaacgtt tagagcgtga gttcaatatt gacctcatca
   101341 tgacagctcc gtctgttatt tacaaagtta atttgaccga cggggagtca atggatgtgt
   101401 ctaacccgtc tgagttccca gatccaacca agattgcgac cattgaagag ccttatgtca
   101461 aggcgcaaat catggtaccg caagagtttg ttggagcagt gatggagcta gctcagcgta
   101521 agcgtgggga ctttgtgact atggactata ttgatgataa ccgtgttaat gtcatctatc
   101581 aaattccact ggctgaaatc gtctttgact tctttgataa acttaagtct tcgacacgtg
   101641 gttatgcaag ttttgactac gaattgtcag aatatcgccc atctaagcta gtgaaaatgg
   101701 atatccttct caacggagac aaggtggatg ccctcagctt tatcgttcat aaggactttg
   101761 cttatgaacg tgggaaactc atcgttgata agctcaagaa aatcatccct cgccaacaat
   101821 ttgaggttcc aatccaagcg gctattggac acaagattgt cgctcgtact gatatcaagg
   101881 cccttcgtaa gaacgtactt gctaaatgtt atggtggtga cgtttctcgt aagcgtaaac
   101941 tccttgaaaa acaaaaagct ggtaagaaac gcatgaaatc catcggatca gttgaagttc
   102001 cgcaagaagc cttcctcagc gtcttgagta tggatgaaga ataagttatc agaaatgcct
   102061 ttccttgttc acaggaaatt tatataagaa tcaatagtaa agcaggctta gatggtctgc
   102121 ttttttagtt tttatactct tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt
   102181 atgtatggtt actgacttcg tcagtttcat ctacaacctc aaaactgtgt tttgagtaac
   102241 ctgcggctag cttcctagtt tgctctttga ttttcattga gtattagaac agaaaaatgc
   102301 ttggagtatt tgtttgtgtg tttattttta tataacaaac tataaacaca acaaaaatat
   102361 aaaaaaagag acaaaaaaag aacagaaagt aattaacaac gatttcatat agtgttatac
   102421 ttatagcata aagttaatgg aaagaaggga aaaccttatg gtattagatt atttctttga
   102481 caaaaacctt gtgttttgct tagaagcgga taatcaagaa caactctttg atcaggttgc
   102541 aactttattg gaagaacgag aaattgtcac tcctacttat cgtgaagcct tgatcacgcg
   102601 tgaaaagtca tttccaactg gtttagatat ggaatttcta ggaaagggct tgccaaatgt
   102661 agcgattcct cacacagata ttgttcataa tctagctgag aaagtggtgg ttgttcgatt
   102721 agaaaaacca gtaacttttc acaatatgat agctcctgat aaggaagtag aagtatccct
   102781 actcttcttt atcattaaca attcaagttc aagtcaaaca aatattctgg ctcagttgat
   102841 ggactttttc acaggaaatg gacatcttga agacctatca aaaatttccg aaccagaaaa
   102901 actttatgct tatatagatg aagcaatcgc ttaatcttgt ctattaaaaa ataaaatcgg
   102961 aggaagtcta gatgattaaa attcttgctg cctgcggtgc aggtgttaac tcaagtcacc
   103021 aaattaaaag tgctctagaa gaagaacttt caaaccgtgg ttacgatgtt cactgtgatg
   103081 cagttatggt gaaagatgta aacgaagacc ttatgaaagg ttacgatatc tttacaccaa
   103141 tcgctgcaac agaccttggt tttgaaccag gtatcccagt tatcgaagct gggccaatct
   103201 tattctgtat cccagcaatg agcgttccag tatttgacaa tattagactt cctgcgaaac
   103261 aaaacatggt atagtagttc tatgaatgat gaagcaagta aacaactaac tgatacacga
   103321 tttaaatgtc ttgttggtgt tcagcgcacg acttttgaag agatgttagc tgtgttaaaa
   103381 acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac
   103441 cttcttatgg ccactcttca ctatgtgcga gaataccgca cttatgaaga aattgcggct
   103501 gattttggtg ttcacgaaag caacttactc cgtcggagcc aatgggttga agtaactctt
   103561 gttcaaagtg gtgttacgat ttcaagaact cctctcagtt ctgaggacac ggtaatgatt
   103621 gatgcgacgg aagtaaaaat caatcgccct aaaaaaacaa ttagcgaatc attctggtaa
   103681 aaagaaattt cacgctatga aggctcaggc gattgtcaca agtcaaggga gaattgtttc
   103741 tttggatatc actgtgaact attgtcatga tatgaagttg ttcaaaatga gtcgcagaaa
   103801 tatcggacaa gctggtaaaa tcttggctga cagtggttat caagggctca tgaagatata
   103861 tcctcaagca caaattccac gtaaatccag caaactcaag ccgctaacag ttgaaga
//
LOCUS       DAVDWM010000002       100272 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVDWM010000002 DAVDWM010000000
VERSION     DAVDWM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 100272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.002"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(34..744)
                     /gene="treR"
                     /locus_tag="VUW49_000108"
     CDS             complement(34..744)
                     /gene="treR"
                     /locus_tag="VUW49_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET7980092.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            929..2896
                     /gene="treP"
                     /locus_tag="VUW49_000109"
     CDS             929..2896
                     /gene="treP"
                     /locus_tag="VUW49_000109"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET7980093.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKTNVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            3074..4699
                     /gene="treC"
                     /locus_tag="VUW49_000110"
     CDS             3074..4699
                     /gene="treC"
                     /locus_tag="VUW49_000110"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET7980094.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGNMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            4841..5089
                     /locus_tag="VUW49_000111"
     CDS             4841..5089
                     /locus_tag="VUW49_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET7980095.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            5296..6090
                     /gene="racE"
                     /locus_tag="VUW49_000112"
     CDS             5296..6090
                     /gene="racE"
                     /locus_tag="VUW49_000112"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET7980096.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            6087..7058
                     /locus_tag="VUW49_000113"
     CDS             6087..7058
                     /locus_tag="VUW49_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET7980097.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            7034..7555
                     /locus_tag="VUW49_000114"
     CDS             7034..7555
                     /locus_tag="VUW49_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7980098.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            7552..8013
                     /locus_tag="VUW49_000115"
     CDS             7552..8013
                     /locus_tag="VUW49_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7980099.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            8004..8744
                     /gene="xerD"
                     /locus_tag="VUW49_000116"
     CDS             8004..8744
                     /gene="xerD"
                     /locus_tag="VUW49_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7980100.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            8744..9472
                     /locus_tag="VUW49_000117"
     CDS             8744..9472
                     /locus_tag="VUW49_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7980101.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLVGRAQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            9491..10060
                     /locus_tag="VUW49_000118"
     CDS             9491..10060
                     /locus_tag="VUW49_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET7980102.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            10047..10769
                     /locus_tag="VUW49_000119"
     CDS             10047..10769
                     /locus_tag="VUW49_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET7980103.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            10769..11011
                     /gene="yidD"
                     /locus_tag="VUW49_000120"
     CDS             10769..11011
                     /gene="yidD"
                     /locus_tag="VUW49_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7980104.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGG"
     gene            complement(11236..12201)
                     /locus_tag="VUW49_000121"
     CDS             complement(11236..12201)
                     /locus_tag="VUW49_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7980105.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSLHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(12297..13049)
                     /locus_tag="VUW49_000122"
     CDS             complement(12297..13049)
                     /locus_tag="VUW49_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7980106.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(13046..14002)
                     /locus_tag="VUW49_000123"
     CDS             complement(13046..14002)
                     /locus_tag="VUW49_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET7980107.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(13992..14951)
                     /locus_tag="VUW49_000124"
     CDS             complement(13992..14951)
                     /locus_tag="VUW49_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7980108.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(15248..15358)
                     /locus_tag="VUW49_000125"
     CDS             complement(15248..15358)
                     /locus_tag="VUW49_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HET7980109.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            complement(15374..16075)
                     /locus_tag="VUW49_000126"
     CDS             complement(15374..16075)
                     /locus_tag="VUW49_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HET7980110.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SHASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLCGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            16476..17540
                     /gene="pepA"
                     /locus_tag="VUW49_000127"
     CDS             16476..17540
                     /gene="pepA"
                     /locus_tag="VUW49_000127"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET7980111.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            17562..17858
                     /locus_tag="VUW49_000128"
     CDS             17562..17858
                     /locus_tag="VUW49_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET7980112.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLASFIKREEAKYRGTATSALMYEELFKML"
     gene            18540..18983
                     /locus_tag="VUW49_000129"
     CDS             18540..18983
                     /locus_tag="VUW49_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7980113.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            18987..19751
                     /locus_tag="VUW49_000130"
     CDS             18987..19751
                     /locus_tag="VUW49_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7980114.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            19765..20493
                     /locus_tag="VUW49_000131"
     CDS             19765..20493
                     /locus_tag="VUW49_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980115.1"
                     /translation="MIEYKNVALCYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            20486..22006
                     /locus_tag="VUW49_000132"
     CDS             20486..22006
                     /locus_tag="VUW49_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET7980116.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKYPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            22350..23156
                     /locus_tag="VUW49_000133"
     CDS             22350..23156
                     /locus_tag="VUW49_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET7980117.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(23322..23858)
                     /locus_tag="VUW49_000134"
     CDS             complement(23322..23858)
                     /locus_tag="VUW49_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7980118.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            23991..24881
                     /locus_tag="VUW49_000135"
     CDS             23991..24881
                     /locus_tag="VUW49_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET7980119.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     VSLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            25125..25478
                     /locus_tag="VUW49_000136"
     CDS             25125..25478
                     /locus_tag="VUW49_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7980120.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSACVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            25479..26516
                     /locus_tag="VUW49_000137"
     CDS             25479..26516
                     /locus_tag="VUW49_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7980121.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(26562..27569)
                     /gene="galR"
                     /locus_tag="VUW49_000138"
     CDS             complement(26562..27569)
                     /gene="galR"
                     /locus_tag="VUW49_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET7980122.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            27733..28911
                     /locus_tag="VUW49_000139"
     CDS             27733..28911
                     /locus_tag="VUW49_000139"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET7980123.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            28930..30411
                     /locus_tag="VUW49_000140"
     CDS             28930..30411
                     /locus_tag="VUW49_000140"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET7980124.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARTNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQAAFMRFVEQVGISLD"
     gene            30481..30885
                     /locus_tag="VUW49_000141"
     CDS             30481..30885
                     /locus_tag="VUW49_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7980125.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGKCVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            31066..31830
                     /locus_tag="VUW49_000142"
     CDS             31066..31830
                     /locus_tag="VUW49_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HET7980126.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            31827..32288
                     /locus_tag="VUW49_000143"
     CDS             31827..32288
                     /locus_tag="VUW49_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HET7980127.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(32339..33601)
                     /locus_tag="VUW49_000144"
     CDS             complement(32339..33601)
                     /locus_tag="VUW49_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET7980128.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(33601..34182)
                     /locus_tag="VUW49_000145"
     CDS             complement(33601..34182)
                     /locus_tag="VUW49_000145"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET7980129.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(34582..34677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(35060..35188)
                     /locus_tag="VUW49_000146"
     CDS             complement(35060..35188)
                     /locus_tag="VUW49_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET7980130.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPNGYFLRFSE"
     gene            complement(35195..35446)
                     /locus_tag="VUW49_000147"
     CDS             complement(35195..35446)
                     /locus_tag="VUW49_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET7980131.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLSL
                     EECQLMLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLY"
     gene            complement(35458..36285)
                     /gene="xth"
                     /locus_tag="VUW49_000148"
     CDS             complement(35458..36285)
                     /gene="xth"
                     /locus_tag="VUW49_000148"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7980132.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            36419..36875
                     /locus_tag="VUW49_000149"
                     /pseudo
     CDS             36419..36875
                     /locus_tag="VUW49_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            37028..37402
                     /locus_tag="VUW49_000150"
     CDS             37028..37402
                     /locus_tag="VUW49_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET7980133.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKP
                     GVLYSSKISSNRVNVALPYVGY"
     gene            37530..39275
                     /locus_tag="VUW49_000151"
     CDS             37530..39275
                     /locus_tag="VUW49_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980134.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESSVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGTI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVEMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            39265..41016
                     /locus_tag="VUW49_000152"
     CDS             39265..41016
                     /locus_tag="VUW49_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980135.1"
                     /translation="MQNKQEQWVVLNRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETSDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            41190..41882
                     /locus_tag="VUW49_000153"
     CDS             41190..41882
                     /locus_tag="VUW49_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7980136.1"
                     /translation="MLKWEDLPVEMKSSEVEFYYQLVSKRKGSLIFKRCLALVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVMDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMLATLLL
                     PAGITSPASINYKDEDTIISQMTEKGLSADQAYVEHVLPEKMRYNLAYLREFSFLGDI
                     KIMFQTVFEVLK"
     gene            41925..43151
                     /locus_tag="VUW49_000154"
     CDS             41925..43151
                     /locus_tag="VUW49_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET7980137.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILHVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTLEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVENKRDLFVASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPAID
                     DEEMYKEFQILSLHGQTEDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            44281..46440
                     /gene="pfbA"
                     /locus_tag="VUW49_000155"
     CDS             44281..46440
                     /gene="pfbA"
                     /locus_tag="VUW49_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET7980138.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            46424..46720
                     /locus_tag="VUW49_000156"
     CDS             46424..46720
                     /locus_tag="VUW49_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980139.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLLEDNKID"
     gene            46958..47617
                     /locus_tag="VUW49_000157"
     CDS             46958..47617
                     /locus_tag="VUW49_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET7980140.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            47642..48709
                     /locus_tag="VUW49_000158"
     CDS             47642..48709
                     /locus_tag="VUW49_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7980141.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELASKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            48813..49823
                     /locus_tag="VUW49_000159"
     CDS             48813..49823
                     /locus_tag="VUW49_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980142.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDEIVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            49835..51526
                     /locus_tag="VUW49_000160"
     CDS             49835..51526
                     /locus_tag="VUW49_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7980143.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            51529..52239
                     /locus_tag="VUW49_000161"
     CDS             51529..52239
                     /locus_tag="VUW49_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7980144.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            52335..52703
                     /locus_tag="VUW49_000162"
     CDS             52335..52703
                     /locus_tag="VUW49_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980145.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            52811..53812
                     /locus_tag="VUW49_000163"
     CDS             52811..53812
                     /locus_tag="VUW49_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7980146.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPGLSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    54024..54253
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            54420..54497
                     /locus_tag="VUW49_000164"
                     /pseudo
     CDS             54420..54497
                     /locus_tag="VUW49_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            54953..56314
                     /gene="trpE"
                     /locus_tag="VUW49_000165"
     CDS             54953..56314
                     /gene="trpE"
                     /locus_tag="VUW49_000165"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET7980147.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKTKSMTRIGE
                     LRP"
     gene            56311..56877
                     /locus_tag="VUW49_000166"
     CDS             56311..56877
                     /locus_tag="VUW49_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET7980148.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            56888..57892
                     /gene="trpD"
                     /locus_tag="VUW49_000167"
     CDS             56888..57892
                     /gene="trpD"
                     /locus_tag="VUW49_000167"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7980149.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            57889..58656
                     /gene="trpC"
                     /locus_tag="VUW49_000168"
     CDS             57889..58656
                     /gene="trpC"
                     /locus_tag="VUW49_000168"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7980150.1"
                     /translation="MSQEFLARILEQKAREVKQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            58643..59242
                     /locus_tag="VUW49_000169"
     CDS             58643..59242
                     /locus_tag="VUW49_000169"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7980151.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQANYLLFDAPVAGSGQPFDWGQLDTTGLPQSFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            59220..60443
                     /gene="trpB"
                     /locus_tag="VUW49_000170"
     CDS             59220..60443
                     /gene="trpB"
                     /locus_tag="VUW49_000170"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7980152.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            60436..61212
                     /locus_tag="VUW49_000171"
     CDS             60436..61212
                     /locus_tag="VUW49_000171"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7980153.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     NLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(61184..61285)
                     /locus_tag="VUW49_000172"
                     /pseudo
     CDS             complement(61184..61285)
                     /locus_tag="VUW49_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            61514..61654
                     /locus_tag="VUW49_000173"
     CDS             61514..61654
                     /locus_tag="VUW49_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980154.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            61691..62245
                     /locus_tag="VUW49_000174"
     CDS             61691..62245
                     /locus_tag="VUW49_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980155.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLMIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            62260..63411
                     /locus_tag="VUW49_000175"
     CDS             62260..63411
                     /locus_tag="VUW49_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET7980156.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(63463..64122)
                     /locus_tag="VUW49_000176"
     CDS             complement(63463..64122)
                     /locus_tag="VUW49_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET7980157.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLSQVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            64192..64590
                     /locus_tag="VUW49_000177"
     CDS             64192..64590
                     /locus_tag="VUW49_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980158.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVALIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NRDIDSISLNVFEANQRAQNLYQKEGFEIV"
     gene            complement(64866..66044)
                     /locus_tag="VUW49_000178"
     CDS             complement(64866..66044)
                     /locus_tag="VUW49_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET7980159.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            66324..67586
                     /locus_tag="VUW49_000179"
     CDS             66324..67586
                     /locus_tag="VUW49_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7980160.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            67589..68485
                     /locus_tag="VUW49_000180"
     CDS             67589..68485
                     /locus_tag="VUW49_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET7980161.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            68500..68817
                     /locus_tag="VUW49_000181"
     CDS             68500..68817
                     /locus_tag="VUW49_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7980162.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            68834..69151
                     /locus_tag="VUW49_000182"
     CDS             68834..69151
                     /locus_tag="VUW49_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7980163.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            69161..70516
                     /locus_tag="VUW49_000183"
     CDS             69161..70516
                     /locus_tag="VUW49_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7980164.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            70839..72314
                     /locus_tag="VUW49_000184"
     CDS             70839..72314
                     /locus_tag="VUW49_000184"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET7980165.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNGVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDIYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            72311..73159
                     /locus_tag="VUW49_000185"
     CDS             72311..73159
                     /locus_tag="VUW49_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET7980166.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            73174..73944
                     /locus_tag="VUW49_000186"
     CDS             73174..73944
                     /locus_tag="VUW49_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7980167.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIEFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(74222..74509)
                     /locus_tag="VUW49_000187"
     CDS             complement(74222..74509)
                     /locus_tag="VUW49_000187"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET7980168.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            74712..74984
                     /locus_tag="VUW49_000188"
                     /pseudo
     CDS             74712..74984
                     /locus_tag="VUW49_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(75094..75297)
                     /locus_tag="VUW49_000189"
     CDS             complement(75094..75297)
                     /locus_tag="VUW49_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7980169.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(75328..75936)
                     /locus_tag="VUW49_000190"
     CDS             complement(75328..75936)
                     /locus_tag="VUW49_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7980170.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(75975..76145)
                     /locus_tag="VUW49_000191"
     CDS             complement(75975..76145)
                     /locus_tag="VUW49_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET7980171.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(76157..76723)
                     /gene="amaP"
                     /locus_tag="VUW49_000192"
     CDS             complement(76157..76723)
                     /gene="amaP"
                     /locus_tag="VUW49_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET7980172.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(76807..76971)
                     /locus_tag="VUW49_000193"
     CDS             complement(76807..76971)
                     /locus_tag="VUW49_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7980173.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            77414..78895
                     /locus_tag="VUW49_000194"
     CDS             77414..78895
                     /locus_tag="VUW49_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7980174.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            79257..80258
                     /locus_tag="VUW49_000195"
     CDS             79257..80258
                     /locus_tag="VUW49_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7980175.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEYKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            80346..81263
                     /locus_tag="VUW49_000196"
     CDS             80346..81263
                     /locus_tag="VUW49_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7980176.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKVDFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            81274..82164
                     /locus_tag="VUW49_000197"
     CDS             81274..82164
                     /locus_tag="VUW49_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7980177.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            82193..83809
                     /locus_tag="VUW49_000198"
     CDS             82193..83809
                     /locus_tag="VUW49_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7980178.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            83819..85138
                     /locus_tag="VUW49_000199"
     CDS             83819..85138
                     /locus_tag="VUW49_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET7980179.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVATKHKDNVGCIVLLGSDNLVEWQFESIFLKGREHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(85346..85418)
                     /locus_tag="VUW49_000200"
     tRNA            complement(85346..85418)
                     /locus_tag="VUW49_000200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85383..85385),aa:Lys,seq:ctt)
     gene            complement(85682..86953)
                     /locus_tag="VUW49_000201"
     CDS             complement(85682..86953)
                     /locus_tag="VUW49_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7980180.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            87223..87462
                     /locus_tag="VUW49_000202"
     CDS             87223..87462
                     /locus_tag="VUW49_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980181.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            87627..87806
                     /locus_tag="VUW49_000203"
     CDS             87627..87806
                     /locus_tag="VUW49_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980182.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            87938..88111
                     /locus_tag="VUW49_000204"
     CDS             87938..88111
                     /locus_tag="VUW49_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET7980183.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            88148..88600
                     /locus_tag="VUW49_000205"
     CDS             88148..88600
                     /locus_tag="VUW49_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET7980184.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            88891..89361
                     /locus_tag="VUW49_000206"
     CDS             88891..89361
                     /locus_tag="VUW49_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET7980185.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            89380..90447
                     /locus_tag="VUW49_000207"
     CDS             89380..90447
                     /locus_tag="VUW49_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7980186.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            90449..90607
                     /locus_tag="VUW49_000208"
     CDS             90449..90607
                     /locus_tag="VUW49_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HET7980187.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            90717..91751
                     /locus_tag="VUW49_000209"
     CDS             90717..91751
                     /locus_tag="VUW49_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7980188.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDLSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            91753..93996
                     /locus_tag="VUW49_000210"
     CDS             91753..93996
                     /locus_tag="VUW49_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET7980189.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDGVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIASDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VILSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            94047..95066
                     /locus_tag="VUW49_000211"
     CDS             94047..95066
                     /locus_tag="VUW49_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7980190.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDRYIWSQIAERSISGS
                     VPVIIYHYVPHFDPGNPIRAKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIF
                     NLMER"
     gene            95079..96029
                     /locus_tag="VUW49_000212"
     CDS             95079..96029
                     /locus_tag="VUW49_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7980191.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHGLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVADGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            96031..96774
                     /locus_tag="VUW49_000213"
     CDS             96031..96774
                     /locus_tag="VUW49_000213"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7980192.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSSLSVLVELEK"
     gene            96785..98581
                     /gene="pepF"
                     /locus_tag="VUW49_000214"
     CDS             96785..98581
                     /gene="pepF"
                     /locus_tag="VUW49_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET7980193.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAQLGE"
     gene            98828..99382
                     /locus_tag="VUW49_000215"
     CDS             98828..99382
                     /locus_tag="VUW49_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET7980194.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            99493..100161
                     /locus_tag="VUW49_000216"
     CDS             99493..100161
                     /locus_tag="VUW49_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET7980195.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT        30582 a        17411 c        21840 g        30439 t
ORIGIN      
        1 ccgaaaaagg ctgagatctg gtgtctcagt cttttatttc ttgcgttttg caaaatctac
       61 aaatctaaat ttctctaact tatggcgact ttcggtaaac tgaaattggc gtccgtcttg
      121 gagatagact tttgatttaa tcgaaacgac ataagggtct ttgccaatgt ccatgagaat
      181 cttgtctcgg tcgcttgagt ggtcaatggt gatttccttc tgagcataat caataaggag
      241 tttgaggcca ttttctatat aagaatagat agactgctca gcaatttggc gagtgagatt
      301 tgggatgagt tccatatcca gatagtccgt atccagaacg gataccagat catccaccac
      361 acgctggcgg accaccttcc aaaccatccg aaactctggg aaaccggtta tcagtgagga
      421 ttttttatca ataataatct tgtccagact gaccacgttg gttttagagc gcagtccaag
      481 ttctttaact agttcttggt agctggttag gttggataca gggaaattga cggtttcttc
      541 tttgacgact tgagaacctt gccctcttat ctttttgatc aatccttcct cttggaggag
      601 agacagggcc tttcggacgg tatcacgact cacttgatat tgctccataa ggtcgtgctc
      661 gctaggaagg aaatctccga cagcgtaagt ctcgttttga atggtttctt ggatttgctt
      721 aaataattgt tggtatttct tcattttgcc tgcttaaata ggtttttagt ctagtttaat
      781 gaacacctgt atctatttta ccatacaagt gctagaaaat ttacaaaaaa acaccctttt
      841 tataccaaac aagttgcaga caagtttggt atcgtttaca atatacttat caaatcaaac
      901 ttgctttgac aagtataagg agaatcaaat gggaaaattt gaacaagaag ccaaagatct
      961 gcttcaggca attggaggca aagaaaatgt gactgccgta actcactgtg cgacacggat
     1021 gcgctttgtt ttaggagatg ataagaagac taatgttaaa gctatcgagt caattccagc
     1081 tgttaaagga acttttacaa atgcaggtca atttcaggta atcattggaa atgacgtgcc
     1141 catcttttat aatgatttta cagccgtttc aggtattgag ggtgtttcca aagaagcagc
     1201 caagtctgca gctaagagta atcaaaacgt ggtccaacgt gttatgacca ctctggcgga
     1261 gatttttact ccgattattc cagccttgat agtcggagga ttgatcctcg gtttccgtaa
     1321 tgtcttggaa ggtgtccatt ggtcgatgtt ggatggcaag accatcacag aatcctctca
     1381 gttttgggca ggtgtcaatc acttcctctg gttgcctggt gaagctatct tccagttctt
     1441 accagtaggg attacttggt ctgtttctcg taagatggga accagccaaa ttttgggaat
     1501 tgttctcgga atctgtttgg tatcgcctca gttgctcaat gcctatgcgg ttgcttcaac
     1561 gccagcagct gatatcgcgg caaactgggt ttggaatttt ggctatttta ctgttaatcg
     1621 tatcggttac caagcccaag ttatcccagc cttgcttgca ggtttgagtc tgtcttatct
     1681 tgaaatcttc tggcgcaagc atatcccaga agtcatttct atgatttttg tacctttctt
     1741 gtcattgatt ccagccttga ttttggctca tactgttttg ggaccaatcg gttggacaat
     1801 tggacaagga ctttcatcag ttgtcttggc aggtttaact ggtccagtta aatggctctt
     1861 cggtgcaatt tttggtgccc tctacgctcc atttgtcatc acaggtctgc accatatgac
     1921 caatgccatt gatacacaat tgattgcgga tgctggtggc actgccctct ggcctatgat
     1981 tgctctttct aatattgctc aaggctcagc cgtgtttgcc tattatttca tgcatcgcca
     2041 tgatgagcgt gaggctcagg tttcacttcc tgcaaccatt tcagcctatc tcggtgttac
     2101 agaaccagct ctttttgggg ttaacgtaaa atatatttat ccatttgttg ctgggatgac
     2161 tggttcagcc cttgcaggca tgttatccgt tacttttaat gtaactgcgg cttctattgg
     2221 tatcggtggt ttgccaggta ttctctctat tcaacctcaa tacatgctgc catttgcagg
     2281 aactatgcta gttgcgattg ttgttccaat gctcttgact ttcttctttc gcaaggctgg
     2341 tctcttcaca aaaatagagg gcgatacgaa cttgcaggca gaattcgttg ctcaagaaga
     2401 agcagaattt gtgaaccatg aaccagtaga acttacttcg gtagaaatta tcagcccact
     2461 aactggccaa gtgaaagaat tgagtcaagc gacggatcct gtttttgcat caggtgtcat
     2521 ggggcaaggt ctggtcattg aaccaagcca aggtgagttg acctctccag ttaatggaac
     2581 agtgacggtt cttttcccta ccaagcatgc catcggcatt gtctctgacg agggagttga
     2641 attgctcatc cacatcggta tggatacagt aggtcttgat ggcaaaggtt ttgaaagtct
     2701 tgtagtccaa ggagatcacg ttatagttgg ccagcaactg attcgttttg atatggatgt
     2761 cattaaggct gcaggtctgg tgacagaaac tcctgttatc atcaccaacc aagatgctta
     2821 tacagcgact attactggaa cttatccgac aacgatccaa gctggagcat ctctcatggt
     2881 cgctacacga atctaatact cttcgaaaat ctcttcaaac cacgtcagct ctatctgcaa
     2941 cctcaaaata gtgttttgag caacctgtgg ctagctttct agtttgttct ttgattttca
     3001 ttgagtataa ttgaaatagc acaaataggg acggaagact gcttcctccc ttttatcaga
     3061 aaaggagaaa cagatgacac ttgataaagg aaaagtagtc tatcaaatct atccaaaatc
     3121 ttacaaagac accactgaaa acggtattgg ggatttccgt gggattatcg aaaaaattcc
     3181 ctatttagcc aaactaggtg tggatatggt ctggctcaat ccattctatc caagccctca
     3241 acgggataat ggttacgata tttcagatta tatggcagtg gatcctcttt ttggtaatat
     3301 ggctgatttt gaggaaatgg tgtgtgtcgg taaagagcac aagattgact ttatgctgga
     3361 catggtactt aatcattgtt cgacagagca tgaatggttt cagaaagccc tagctggtga
     3421 taagtattat caagactttt tcttcatcca agaccaacca acagattggc agtctaagtt
     3481 tggtggttct gcatgggcgc ctttcgggga tactgggaaa tactatcttc acctatttga
     3541 tgagacccag gctgacctta actggcgcaa ttccaatgtc cgtaaggagc ttttcaaggt
     3601 tgttaatttc tggcgcgaca agggtgtcaa aggtttccga tttgatgtga tcaatttgat
     3661 cggtaaggac gaggtttcag tggactgtcc tgaaaatgaa gggaagccag cttatacaga
     3721 caaacccatc gttcataact atttgcgtat gatgaaccaa gccacttttg gatctgacga
     3781 tagctttatg acagttgggg aaatgtcttc taccaccatg gaaaactgtg tcctctattc
     3841 gtcgcctgat cgtcaggaat tatccatgac ctttaatttt catcacctca aggtggacta
     3901 taaggatgga caaaagtgga ccttggctcc ctttgatttt gaagagttga agagtcttta
     3961 ccatagttgg ggtaaggaaa tgagtgataa agacggttgg agtgccctct tttggaacaa
     4021 tcacgatcaa ccacgtgcct tgaaccgttt tgtcgatatt caaaacttcc gcaaggaagg
     4081 agccaccatg ctagcagcca gcattcacct gtcacgtggg acaccttata tctacatggg
     4141 tgaggaaatc gggatgattg acccagacta tgattctatg gctgattatg tggatgtcga
     4201 atcgcttaat gcctatcaga tgctcttaga agaaggaaag agtcagcaag aagccttcca
     4261 gattattcaa gctaagtcgc gtgataattc acgtattccc atgcagtggg atacttctga
     4321 aaatgcagga ttttcaacag gcactccatg gttgaaagtt ggtaaatcct ataaagatat
     4381 caatgtagaa aatgaaatca aaggtctgat tttcaccttc tatcaagact tgattcgact
     4441 gcgtaaggaa atgccaatca tttcagaagg aaattacaaa ccagcctttg aagatagcaa
     4501 acaagtctac gcttttgaac gccagtttga ggatcaaaag ttactagtgc tcaataactt
     4561 ttatgccaaa gaagtggaaa tcgacttgcc agcagtctac caaaatggac agattttgat
     4621 ttcaaactat gaagatgcag aagtgtctga gaaaattcta ctcaaaccct atcaaacact
     4681 ggctatttat gtaaattaaa cttagcaagg agacctcttt ttcagaaaag cagggttctt
     4741 tttgctgtct attccatcct tgaaagtgta gaacaaactg gtcagggtgg ctagtttgtg
     4801 gtataatgaa agggactcaa aaagaaacag gagaaaaatg atggatttac ttttagcaat
     4861 tgtattgatt gtgctagctt ttctaggagg agctcttgga ggaatgtact tggttcgtaa
     4921 gcaaatcgaa aaagaatttg ctgacaaccc acgtttgaat gctgaagcag ttcgtactct
     4981 tttgagtgca aatggtcaaa aaccaagcga agctaaggta caacaagttt accaccaaat
     5041 catccgccaa caaaaggcag cccttgctaa caataaaaag aaaaaataaa taaggaaaag
     5101 tctgggatga aagttccaga cttctcacta tgttggttat ggtttaggga tgagactttt
     5161 tccttgcatt ctattgatat ttgattatga ttaagagaga ggtagttgac attctatcaa
     5221 gctgtcggtc agttcgttca gtacaaggaa tcataatgaa cgatcaatca gaaaaaagac
     5281 tagaaagaag actgtatgga taatcgacca attggttttt tggattcggg tgtcgggggc
     5341 ttgaccgttg tgcgcgagct catgcgccag cttccccatg aagaaatcgt ctatattgga
     5401 gattcggcgc gggcgcccta tggcccccgt cctgctgagc aaattcgtga atatacttgg
     5461 cagctggtca actttctctt gaccaaggat gtcaaaatga ttgtcattgc ttgtaacact
     5521 gcgactgcgg tcgtctggga agaaatcaag gctcaactag atattcctgt cttgggtgta
     5581 attttgccag gagcttcggc agccatcaag tccagtcaag gtgggaaaat cggagtgatt
     5641 ggaacgccca tgacggtaca atcagacata taccgtcaga aaatccatga tctggatccc
     5701 gacttacagg tggagagctt ggcctgtccc aagtttgctc ccttggttga gtcaggtgcc
     5761 ctgtcaacca gtgttaccaa gaaggtggtc tatgaaaccc tgcgtccctt ggttggaaag
     5821 gtggatagcc tgattttggg ctgtactcat tatccactcc ttcgccctat tatccaaaat
     5881 gtgatggggc caaaggttca gctcatcgat agtggggcag agtgcgtacg ggatatctca
     5941 gtcttactca attattttga aatcaatcgt ggtcgcgatg ctggaccact ccatcaccgt
     6001 ttttacacaa cagccagtag ccaaagtttt gcacaaattg gtgaagaatg gctggaaaaa
     6061 gagattcatg tggagcatgt agaattatga caaataaaat ttatgaatat aaggatgacc
     6121 aggactggta tgttgggtct tatagtattt ttggtggcgt taacagtttg agcgactata
     6181 agacagattt tcctctgttt gaattctcca aaatatttgg agatgaagag tatggtttcc
     6241 cgctttcagt tactgtttta cgctatggtt ctacctaccg tttgttctcc tttgtggtag
     6301 acatgcttaa tcaagaaatg ggacgaaact tggaagttat tcaacgtcat ggggccctgc
     6361 tcttggttga aaatgggcaa ctcttgtatg tagaattgcc taaagaaggg gtcaatgttc
     6421 atgatttctt tgagacaagc aaggtcagag aaaccttgtt gattgcgact cgtaacgaag
     6481 gtaaaaccaa ggaattccga gctatctttg ataagttagg ctacgatgtg gaaaatctta
     6541 atgactaccc tgacctgcct gaagtagcag aaacaggtat gacctttgaa gaaaatgccc
     6601 gccttaaggc agaaaccatt tctcaattaa cgggcaagat ggttttggca gatgattctg
     6661 gtctcaaagt cgatgtcctt ggtggcttac caggcgtctg gtcagctcgt ttcgcaggtg
     6721 tgggagcaac tgaccgtgaa aataatgcca aactcttgca cgaattggcc atggtctttg
     6781 aactcaagga ccgctcggct cagttccaca caaccctagt cgtagccagc ccaaataagg
     6841 aaagtttagt tgttgaagca gactggtcag gttatattaa ctttgaacct aagggtgaaa
     6901 atggctttgg ctatgatccc ctcttccttg taggagagac aggtgagtca tcagctgaat
     6961 taaccctgga agaaaaaaat agtcaatctc accgtgcctt agccgttaag aaacttttgg
     7021 aggtatttcc atcatggcaa agcaaaccat cattgtaatg agcgattccc atggcgatag
     7081 cttgattgtg gaagaagtcc gtgatcgcta tgtgggcaaa gtcgatgccg tttttcataa
     7141 cggcgattct gaactacgtc cggattctcc actttgggag ggcatccgcg ttgttaaagg
     7201 gaacatggac ttctacgccg gctacccaga acgtctggtg actgagcttg gttcgaccaa
     7261 gattatccaa actcatggtc acttgtttga catcaatttc aactttcaaa agttggacta
     7321 ctgggctcag gaggaagagg ccgctatctg cctctatggt cacttgcatg tgccaagtgc
     7381 ttggatggaa ggcaagatcc tctttctaaa tccaggttct atcagtcaac cacgaggtac
     7441 catcagagaa tgtctctatg ctcgtgtgga gattgatgat agttacttca aagtggactt
     7501 tttgacacga gatcacgagg tgtatccagg tttgtccaag gagtttagcc gatgattgcc
     7561 aaggagtttg agactttctt gttggggcag gaggaaactt ttttgacccc tgctaaaaat
     7621 ctagctgtgt tgattgatac ccacaatgcg gatcatgcga ccctcttgct cagtcagatg
     7681 acctataccc gtgttcccgt tgtgacagat gaaaaacagt ttgttgggac gattggactc
     7741 agagatatta tggcttatca gatggagcat gacttgagcc aagaaatcat ggcggatacg
     7801 gatatcgttc atatgacaaa aacggacgta gcggttgttt cgcctgattt caccattacg
     7861 gaggtcttgc acaagctagt agatgagtcc ttcttaccgg ttgtggatgc agagggtatt
     7921 ttccaaggga ttattacgcg caagtccatc ctcaaggccg ttaatgccct cttgcatgac
     7981 tttagtaagg aatatgagat tcgatgccaa tgagagacag gatttcagcc tttttagagg
     8041 aaaagcaggg cttgtctgtc aattccaagc agtcctataa gtatgatttg gagcaatttt
     8101 tagacatggt aggtgagcgg atttctgaga ccagtctcaa gatttaccaa gcccagctag
     8161 ccaatctaaa aatcagcgcc cagaagcgaa agatttcggc ctgtaaccaa tttctatact
     8221 ttctctatca aaaaggagag gtggacagct tttaccgctt ggaattagcc aaacaagctg
     8281 aaaagaagac ggaaaagcca gagattctat acctagactc tttttggcag gaaagcgacc
     8341 atccagaggg ccgcttgcta gcgctcttaa tcctagaaat ggggctcttg cccagtgaga
     8401 ttttagccat caaggttgcg gacatcaatc tggattttca ggtgttgcga atcagcaagg
     8461 cttcccaaca gaggattgtc accattccca cggccttgct ttcagaattg gaacccttga
     8521 tggggcagac ctatcttttt gaaagaggag agaaacccta ttctcgtcag tgggcctttc
     8581 gtcagttaga atcttttgtc aaggagaaag gttttccatc cttatcagct caagtcttac
     8641 gtgaacagtt tattctaaga caaatagaaa acaaggtcga tttgtacgaa attgcaaaaa
     8701 aattaggatt aaaaacagtc ctgaccttag aaaaatatag ataatggata ttaaattaaa
     8761 agattttgaa ggacccctgg acttgctctt gcatctggtt tctaagtacc agatggatat
     8821 ctacgatgtg cccattacgg aagtcatcga acagtatcta gcctatgtct caaccctgca
     8881 ggccatgcgt ctggaagtga cgggtgagta catggtcatg gctagtcagc tcatgctgat
     8941 taagagtcgt aaactccttc cgaaggtagc agaagtgaca gacttggggg atgacctgga
     9001 gcaggacctc ctctctcaaa tcgaagaata tcgcaagttc aagctcttgg gtgagcactt
     9061 ggaagccaag caccaagaac gggcccagta ttattccaaa gcgccgacag agttgattta
     9121 cgaagatgcg gagcttgtgc atgacaagac gaccattgac ctctttttgg ctttttcaaa
     9181 tatcctagcc aagaaaaaag aggagtttgc acaaaatcac acgacgatct tgcgggatga
     9241 gtataagatt gaggacatga tgattatcgt gaaagagtcc ttggttggac gagctcaatt
     9301 gcgcttgcag gatttgttca aggaagccca gaatgtccaa gaggtcatca ccctcttttt
     9361 ggcaacccta gagttaatca aaacccagga gttgatcctc gtgcaagagg agagttttgg
     9421 agatatctat ctcatggaaa agaaggaaga aagtcaagtg cctcaaagct agacttgata
     9481 gagaggaaag atgagtactt tagcaaaaat agaagcgctc ttgtttgtag cgggtgaaga
     9541 tgggattcgg gtccgccagt tagctgaact cctctctctg ccaccgacag gcatccagca
     9601 aagtttagga aaattagccc agaagtatga aaaggaccca gattccagtt tggctttgat
     9661 tgagacaagt ggtgcttata gattggtgac caagcctcaa tttgcagaga ttttgaagga
     9721 atactctaag gcgcctatca accagagctt gtctcgggct gcccttgaga ccttgtccat
     9781 tattgcctac aaacagccga ttacgcggat agaaattgat gccatccgtg gagttaactc
     9841 gagtggagcc ttggcaaagt tgcaggcttt tgacctgata aaggaagacg ggaaaaagga
     9901 agtattgggg cgccccaacc tctatgtgac tacggattat ttcctagatt acatggggat
     9961 aaaccattta gaagaattac cagtgattga tgagcttgag attcaagccc aagaaagcca
    10021 attatttggt gaaaggatag aagaagatga gaatcaataa gtatattgcc cacgcaggtg
    10081 tggccagtag gagaaaagca gaagagctga ttaagcaagg cttggtgacg gttaacggcc
    10141 aagtggtgcg tgaactagca accactatca agtcaggcga caaggtcgaa gttgaaggtc
    10201 aacctatcta caacgaagaa aaggtctact atctgcttaa caaaccacgc ggtgtgattt
    10261 ccagtgtgac agatgataag ggtcgcaaga cggttgtcga cctcttgccc aatgtcaaag
    10321 agcgtattta ccctgtgggt cgtttggact gggatacatc aggtgtcttg attttgacca
    10381 atgatgggga ctttacagac gagatgattc accctcgtaa tgagattgac aaggtttatg
    10441 tcgcgcgtgt taaaggtgtg gccaataagg acaatctccg ccccttgacc cgtggtcttg
    10501 agattgatgg taagaaaacc aagccagctg tttatgaaat tctcaaagtg gacccagtca
    10561 aaaatcgctc tgtggtgcag ttgaccatcc atgaagggcg taaccatcag gttaaaaaga
    10621 tgtttgaagc tgttggtctc caagtagata agttgtctcg gactcgtttc ggacacctag
    10681 acttgacagg actccgtcca ggagaatccc gtcgtcttaa taaaaaagaa atcagccaac
    10741 tacacaccat ggctgtaact aagaaataat gaaacgaatt ttaatagcgc ctgtgcgctt
    10801 ttaccaacgt tttatctcac cagtctttcc accctcttgt cgctttgagc tgacttgttc
    10861 caactacatg attcaggcta ttgaaaaaca tggttttaag ggggtattga tgggcttggc
    10921 tcggatttta cgttgtcatc cctggtcgaa aacaggtaag gatcccgttc cagaccactt
    10981 ttcccttaaa cgaaatcaag aagggggatg aggtggggta aatagatttc aaaatgataa
    11041 aaacgcatcc tatcaggttt gagtaaactt gataggatgc gttttagaat gtcaaaattt
    11101 tatactcttc gaaaatctct tcaaaccgcg tcagctttca tctgcaacct caaaacagtg
    11161 ttttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg agtattaaat
    11221 tgagtttgaa gtggcttatt tcaaagcttt ttgtatgtct tcaatcatga gttttgttga
    11281 ttcaagtccg cctccgctta gataccagag gtctggtgtt agttggataa tcttaccatt
    11341 tttagcagca ggtgtttcag cgataagggc attttctagg acaccgtcgt tgctagagtt
    11401 gtccccaccg atggcaaggg tacggttgat gacaaagagg atgtcagggt tgatttcttt
    11461 gacactttca aagctgactt cttgtccgtg gagtgagtct tcaaattttg tatcagttgg
    11521 tttgaatttc aaggtttggt acaagaaaga gaaacgagat ttggcaccaa aggctgccat
    11581 ttttccttca ttaaggagga tcgcaagggc ttttttgtca gagctttcat ttttagtagc
    11641 gacttcttgg atgctcttgt ctagcttggc caattcttcc ttggctttct gtgtaccagt
    11701 ttcgccgaag gcacttgcta aggattcgat attagccttg gtagaagtcc agtagtcatc
    11761 cttgcttgct tggaagagaa cggttggggc gatttctttg aatttgtcta cgaatttttg
    11821 tgtacgtggt gaagcgataa tcaaatcagg ctcaagggcg gcgatagctt ctaaatcagg
    11881 ttctttcata gaaccaacat ttttgacagt tcccactagg tcttttagat aagtcggaac
    11941 agtttttgta ggcattccga cgatattttt ttcaaatcct aaagcgcgaa tagtatccgc
    12001 agcgccgagg tcaaaggtca caatcttttc aggaactttg gaaagtttga cctcgtccag
    12061 tgaactttta atggttatct ctgttggagc agagctactg gtctctgtct gactagtgct
    12121 tgagtttgta ctacatgcac caagtaggag caagaagctg gccactaggg cagtgaaata
    12181 aagtttaagg gatgttttca taatttctcc tttttaaaat gtgataacga tttagggagt
    12241 ctcttaatct tattgactaa gagactgaag gttctctaac ttgagctttt atgttactag
    12301 ctatagatac agatcttttt gtcattgata tcagctagcg tgatgggaat ctcataaagt
    12361 tgactgagca ggtcagcctg catgatttga tcggttcttc ccttgctaaa gacctggccg
    12421 tccttgaagg cgacaatttc atctgcatac tgactggcca tgttgatatc gtggaggacg
    12481 atgataatgg tcttgccgag ttcctccacc agtcgtcgaa gaatctgcat catgctgacg
    12541 ctttgcttga tatcgagatt gttgagtggt tcgtccagca agataaagtc cgtatcctgg
    12601 gccagtacca tagcgataaa gacgcgctgg agttgccccc ctgacaggct attgatgtag
    12661 cggtctttta agttggtcag ttccaaatag ttcagagttt ctcggatttt ttcccagtct
    12721 tctgatctaa gtcgacctcg gctgtaggga aaacgtccaa aactgaccag ttcttcaaca
    12781 gtcaatttgg cttggtaatt gattttctgt tttaggatgg ttagttcttg ggccagttct
    12841 tgcgaattcc agctctcgat ttcacgtcct ttgatactga gaactccctg atctttcttg
    12901 gttagtctgc tcatgatgga gaggagagtc gattttccag caccatttgg accaataaag
    12961 gctgtcagtt tttgaggact gacttcaagc gaaatgcctt gcaaaatatc ctgtttttga
    13021 atggatttgt caatgttttc cagtttcact gacgagccct cctatatagt aagataaaga
    13081 ataagaagcc acccacactc tcaatgatca tactgatacg aatttccagt gcaaagactc
    13141 gttcaatcaa ggcttgcccc aaggttaagc taataaatcc aacaagaatg gccactataa
    13201 agagtaactt gtgctgatag tctttgacaa tcaggtaggt gaggttggcc agcataaagc
    13261 cgaagaaggc cataggtcct accaaggcag tggccgttga ggtcaaaagc acgattcccc
    13321 agaggagctc tttctgttct ttttcaacat cgagtcccaa tatctgagcc gtttctcttt
    13381 gcaggtgcaa gacatctaga acgactgctt ttcgaaagaa aaagattgtc aaagcgagga
    13441 tgatcagaga accgatggct aggatggaag tgttaagatg ttgaaaggag gcaaaaagac
    13501 tattttgcag tttatcgtat tcgtttggat ccattaggac ttgaaggaag gtgctgatat
    13561 ttcgaaagag acttctgagc gctagacaga tcagcaggat gaagaccagg tcttgcttca
    13621 tcagtgtctt caagtaacct tgtaaggcga gaaagaagaa ggactggaca agaagtaaga
    13681 ctaggaattc taagataggg gatttgccaa gttgaagaaa cttgctttca aaaaccagta
    13741 gtagggtttg tagtaggacg tagaaggatt caattcccaa aatactaggc gtcaggaagc
    13801 gattttccgt cagggtttga aaactaatgg tcgaaatccc agtcgcgatg gctaccaaga
    13861 gataaacgat gatcttttgg gaacgcaact tccaagcaaa ggctgacaag tgagtgatgg
    13921 gccaaaagta gagaagacaa gctccgatgg caagaataat gagaatccag aagagcttgg
    13981 tatgtttgct tttagtctgc atcttttcgt ccccctctcc agagaagtag gataaagacg
    14041 agactaccga tgattcctag caagagactg acagacaact catagggcct aatcagaact
    14101 cgggatagga tatcgcaagc cagaactaga ttggcaccaa ccagtgcgac catgagtttg
    14161 gtttgactta gattatctcc atagcgcttg cgaacaagat tgggaacgat aactccgaga
    14221 aatggtaagc cacccacggt aatcatggtg acgcttgtcg ttagcgccac cagaaagagg
    14281 gccagttttt caagtaggga gtaggaaatc cccaaactct cgctggtttc tttccccaga
    14341 ttcatgatgg tgaaggtttg ggataatttc caaacggtta tcaggatgat gaggcctaag
    14401 aagagccact catactgatg ggtctgaatc atggagaagg agccctgggt ccaggcagtc
    14461 atactctgaa ccagattgaa acgataggcg ataacttctg tgactgagcc gataatcccg
    14521 ctatagatga tcccaatcag aggcaacatc cacctttcct ttacagtaaa aatggtcata
    14581 aaggctagga agaagagggt gaatacgatg gatgaaacaa aagcgaagag catcttttgg
    14641 gtcagactag ccgatggaaa gacaaaaagg ctcagcacca ttcccagttt ggcggcttca
    14701 gtcgttccaa ctgtactcgg tgcagcaaac tgattttggg taatagtctg catgagaagg
    14761 cctgccatac tcatactaga ggcagtcagg agaatactga tagttcttgg gagacgggac
    14821 tcttgaaaaa ggagccaggt ctgctggtcg aaatcaaata gctttcccca tgaaaaatca
    14881 ctggtcccaa tgctaataga gagaaagact aggagtagaa gtaagccaat taaataatga
    14941 gaaagtttca taccccgtcc tttcatgtag atttggtatc gaaagatatc tgcggatata
    15001 aatgtaacat tatttttcta atctgtcaat aaaatttctg acaatttaat aaatacaaca
    15061 aggagagagc aacaagactt tctcctttgt tatcctattc taaaatgttt ttaccttaat
    15121 ctgataaaat aatatcttcg agggagtagc tagccgtcca atcaagatat tgtttagctt
    15181 ttgaagcatc tgctaggaca ctggctgggt cactagcacg tcgagcaaca atctcgtgtg
    15241 ggatttttta atttagtaat tcttcagcag ttttaaagat ttctttgata gtatagcctt
    15301 ttttagttcc taagttaaag atttgagaag aactgtcttc ttgaaatagg tagttcattc
    15361 ctttaacatg agcctatgca aggtccaaga cataaatgta atctcgaata catgaaccgt
    15421 cacgtgtatc gtagtcatct ccaaatattt ttaagctatc attttgtccc aatgcggtct
    15481 tgttgatatt tggaatgatg tgagttggat ttttcacacg cagaccgttt gaagcatcca
    15541 tttcagcccc agcaacatta aagtaacgga aaataacata tttccagtcg tagcgattgg
    15601 ccatccagta aatcattcgt tcgcccatca gttttgtctc tgcataaggg ttgacagggt
    15661 cgagcagggt atcttcagtc accggcttgt caatacagtt atttccacag agagaagcag
    15721 tcgaagagaa catgattttt tgaatgccaa cttcagataa gactttgaga acttggttca
    15781 taccagcaac gttggcagtg aagtatttac ttggattttc aatactttcg ctcacaacaa
    15841 tttcacctgc acaatggagg acagcatcaa tctgattttc ttctaagtaa gcctttaagg
    15901 cgctagcatc ataaacatcc agttctttaa agctagcatg actatccaca gccgctcgat
    15961 ttcctgtgga gagattatcg aggacatgca cctgatagcc agcatttaaa agagctttaa
    16021 cggtatggga gccaatgtag ctagtcccac ctgtaacaag aattgttttg gtcatgattt
    16081 tttccttttc tagttttgta ctatagtaaa ttgaaataag atatgaacaa ctctattagg
    16141 aaagtcaaat taatttctag aaatgtttta gcagctacag cgtactattc caaactcaac
    16201 caactatata tttaaagaaa gaagaaaggg aagtcaacta ttttctgtaa atttcatgaa
    16261 aaatccaaac aactttgatg gtgtattgtt tttttgtttg gaaataaata ttcgagttaa
    16321 ttttggaatg tttactagat tttacactac atgcaagaga atactatcct tttctaagat
    16381 agagcaggac taagatacta acttagaaat ccaccttccc actatccttc tcctataaaa
    16441 agtggtaaaa tggatgaaag aaagaaggaa ctgaaatgac aacattattt tcaaaaatta
    16501 aagaagtaac agaacttgct gcagtctcag gtcatgaagc gcctgtccgt gcttatcttc
    16561 gtgaaaagtt gacaccgcat gtggatgaag tggtgacaga tggcttgggt ggtatttttg
    16621 gtatcaaaca ttcagaagct gtggatgcac cgcgcgtctt ggtcgcttct catatggacg
    16681 aagttggttt tatggtcagc gaaatcaagc cagatggtac cttccgtgtt gtagaaatcg
    16741 gtggctggaa ccccatggtg gttagcagcc aacgtttcaa actcttgact cgtgatggtc
    16801 atgaaattcc tgtgatttca ggttctgttc ctccgcattt gactcgtgga aaggggggac
    16861 caaccatgcc agccattgcc gatatcgttt ttgatggtgg ttttgcggac aaggctgagg
    16921 cagaaagttt tggcatccgt cctggtgata ccatcgtacc agatagttct gcaattttga
    16981 cagccaatga aaaaaatatc atctcaaaag cttgggataa ccgctacggt gtcctcatgg
    17041 taagcgagct agctgaagct ttatcaggtc aaaaactcgg caatgaactc tatctgggtt
    17101 ctaacgtcca agaagaagtt ggtctgcgtg gcgctcatac ctctacaacc aagtttgacc
    17161 cagaagtctt cctcgcagtt gattgctcac cagcaggtga tgtctacggt ggtcaaggca
    17221 agattggaga tggaaccttg attcgtttct atgatccagg tcacttgctt cttccaggga
    17281 tgaaggattt ccttttgaca acggctgaag aagctggtat caagtaccaa tactactgtg
    17341 gtaaaggcgg aacagatgca ggtgcagctc atctgaaaaa tggtggtgtc ccatcaacaa
    17401 ctatcggtgt ctgcgctcgt tatatccatt ctcaccaaac cctctatgca atggatgact
    17461 tcctagaagc gcaagctttc ttacaagcct tggtgaagaa attggatcgt tcaacggttg
    17521 atttgattaa acattattaa accataaggg aggtggtagg gatggtagaa ccaaacctag
    17581 aaagccttat aaaagatctt tacaatcatg ctcgacatga tttgagtgaa gatttagttg
    17641 ctgctctcct agagactact aaaaaactgc ctactacaaa tgagcaattg caggcagttc
    17701 gtctctcagg cctggtcaat cgtgaattgc tcctaaatcc caaacatcca gcacctgagt
    17761 tgctcaactt ggctagcttt atcaaaagag aagaagccaa gtacagagga actgcgactt
    17821 ctgcgcttat gtatgaggaa ctctttaaaa tgctttgatt ccattgtgga tcaaagcatt
    17881 ttttataaga cctatctcgt gagcgttcaa atagtagtaa caaggaatca tgagaagtca
    17941 gccgagacca tagtagtgaa gaaacttctg taatggaagt ggagtgaagt gaggaatcgt
    18001 caaacggtag gggataggct gtttgaaggg tgctacgaaa aagatagggt aaggtgaaat
    18061 tatagcgaga tgaaataaga catgaacaaa ttgattagga aaatcaaatg aatttataga
    18121 aatcttttag cagtcatggc gtcctattct agtttcaaaa tgctataaat acgagcaaat
    18181 tgattgggaa attcaaatcg ctttctatca atgttttaga agatacagtg ccctagtcca
    18241 acttcaatat actataaaag aacagatgga agcaattatg atttacttct atctttaaaa
    18301 taaatgctct gataggaaaa atgtgtcccc tcataaaata taggatagta cggatggaag
    18361 gcttttgtag actggaggac gaaggagttt agaaactctc gttaaaagtt agttgagccg
    18421 gagtatggtg gtgtggataa ttatcaaaaa ttatttctaa atcgaaatag ttgacagtta
    18481 gaacgatgag tgttacaatt gttttattca tttcgatatc gaaataaatt ggaggtgtta
    18541 tgaaagatgg tcatttgcta gcccatcata ttcgtttgtt gaatgggcgg atttttcaaa
    18601 agttactgag tcaagatcct gaggctcttt ataggggtga acagggcaag attttagcgg
    18661 ttttatggaa tagtgaaact ggctgcgcaa ctgcgacaga tatcgcgctt gcgactggac
    18721 ttgcgaataa tacgctgacg actatgataa aaaagctaga ggaacaaaag cttgtaattg
    18781 ttagtccgtg tggaaaagac aagcgtaaga agtatttagt tttaacggag ttaggcaagt
    18841 cccagaaaga agtggggcat cgtgtcagtc agaaattgga tactatcttt tacaaaggat
    18901 tttcagagga agaaattcac caatttgaag gttttcaaga aagaattttg gcgaatctga
    18961 aagagaaggg aaatgaggtt tagaatatgt cacaacaagt gaaaaatgct cacaacctat
    19021 acatccatgc cattcaagac ggacgagttg ctgaggctca agcccagtct gtaggggata
    19081 cctatatcca acattcgaca ggtgttcctg acgggaaaga aggatttgca gccttctttg
    19141 cagatttctt tgagcgtcat ccagagcgtc agattaagat tgtccgcacc attgaggatg
    19201 gcaatctggt ctttgttcat gttcatcaat atctgaatgg tggagaagct caatgggtga
    19261 cgacggatac tttccgtgcg gatgagaatg gtcgtattgt tgagcattgg gatgtcattg
    19321 actactatcg aacacctgaa aatgaccaac tagatcaaat ttttggagat tttgaaatca
    19381 aggatttgga caagaaagca gaaaataaaa agctggttcg ccgtttcttg acagaaattt
    19441 tccaaaatgg ggagctagag cagtggagtg attatgtggc agacgatttg attcagcata
    19501 atcatgatat tggacaagga agtgctgctt ataaaaacta tgtggctgaa tatagtgtca
    19561 cttttgactt tgttttccaa ctcttaggac aaggaaacta tgtggttagc tatggtcaga
    19621 ctcagattga tggcgttgct tatgcccagt acgatatctt ccgtttaaag aacgggaaaa
    19681 ttgtggagca ttgggataat aaggaagtca tgcctaaggt agaagacttg accaatcgag
    19741 ggaagtttta aatgaggaca aagaatgatt gaatacaaaa atgtagcact atgctacaca
    19801 gaaaaggatg tcttgagaga tgtcaactta cagattgagg atggggaatt tatggtttta
    19861 gtagggcctt ctgggtcagg taagacgacc atgctcaaga tgattaaccg tcttttggaa
    19921 ccaactgatg gaaatattta tatggatggg aagcgcatca aagactatga tgagcgtgaa
    19981 cttcgtcttt ctactggtta tgttttacag gctattgctc tttttccaaa tctaacagtt
    20041 gcggaaaata ttgctctcat tcctgaaatg aaggggtgga gcaaggaaga aattacgaag
    20101 aaaacagaag agcttttggc taaggttggt ttaccagtag ctgagtatgg gcatcgctta
    20161 cctagtgaat tatctggtgg agaacagcaa cgggtcggta ttgtccgagc tatgattggt
    20221 cagcccaaga ttttcctcat ggatgaaccc ttttcggcct tggatgctat ttcgagaaaa
    20281 cagttgcagg ttctgacaaa agaattgcat aaagagtttg ggatgacaac gatttttgta
    20341 acccatgata cggatgaagc cttgaagttg gcggaccgta ttgctgtctt gcaggatgga
    20401 gaaattcgcc aggtagcgaa tcccgagaca attttaaaag tgcctgcaac agactttgta
    20461 gcagacttgt ttggaggtag tgttcatgac taatttaatt gcaacttttc aggatcgttt
    20521 tagtgattgg ttgacagctc tatctcaaca tttgcagttg tcacttttga ccttgttact
    20581 agctattttg cttgcgattc ccttggctgt ttttcttcgc tatcatgaga agctggccga
    20641 ctgggtcttg cagattgcag gtattttcca gaccatcccg tctctggcct tgttggggct
    20701 ctttatccct ttgatgggaa ttgggacctt gccggctttg acagctctag tgatttatgc
    20761 gatttttccg attttacaaa atactattac tgggctgaag ggaattgatc cgagtcttca
    20821 agaggctggg attgcctttg ggatgaccag atgggagcgt ctcaagaagt ttgaaattcc
    20881 acttgccatg cctgttatca tgtctggaat tcggacggca gcggtcttga ttatcggtac
    20941 ggctaccttg gcgaccttga ttggggcagg gggactgggt tcctttatcc tgttgggaat
    21001 tgaccgtaat aatgccagtt tgattttgat tggggccctt tcttctgcag tgctagctat
    21061 tgcctttaac ttcctactaa aagtaatgga aaaagcgaaa ttgcggacga ttttctctgg
    21121 ttttgccttg gtggctttat tactgggtct gtcttatagt ccagctcttt tggctcaaaa
    21181 agagaaggaa aacttgatta ttgctgggaa aataggccca gaaccagaaa ttttggctaa
    21241 tatgtataag ttgctgattg aagaaaatac cagcatgact gcgactgtta aaccgaattt
    21301 tgggacgaca agtttccttt atgaagctct gaaaaaaggt gatattgaca tttatcctga
    21361 atttaccggt acggtgactg aaagtttgct tcaaccatca cccaaggtga gtcatgagcc
    21421 agagcaggtt tatcaagtgg cgcgtgatgg cattgccaag caggatcatc tagcctatct
    21481 caaacctatg tcttatcaaa atacctatgc tgtagctgtt ccgaaaaaga ttgctcaaga
    21541 atatggcttg aagaccattt cggatttgaa aaaagtggaa gggcagctga aggcaggctt
    21601 tacgcttgag tttaacgacc gtgaagatgg aaataagggc ttgcaatcaa tgtatggtct
    21661 caatctcaat gtggcgacta tgcagccagc tcttcgttat caggcaattc agtcagggga
    21721 tattcaaatc acggatgcct attcgactga tgcggaattg gagcgttatg atttacaggt
    21781 cttggaagat gacaagcaac tcttcccacc ttatcaaggg gctccactca tgaaagaagc
    21841 tcttctcaag aaatacccag agttggaaag agttcttaat acattggctg gtaagattac
    21901 agaaagccag atgagccagc tcaactacca agtcggtgtt gaaggcaagt cagcaaagca
    21961 agtagccaag gagtttctcc aagaacaagg tttgttgaag aaatgatggg acagagactt
    22021 taaacatact gaactcaact tccttaaacg actagataaa agaatgctca gacaatttcg
    22081 tctgggcttt ttgatgtgag aatgactgaa tgtcacttaa aatggaaaga aagagaaaat
    22141 tttcatgatt ttctaaaatt ttactgtaaa gacagttgac tattcataaa ataggtgtat
    22201 aatattttgt gaactactta acaaataaga ggtttcagct catgtcgact aaggttagaa
    22261 atcttgtgta taggcagttc attatctaat agagcgttgc gtccgaaagt ctatccagac
    22321 acggctcttt aaaaacaaaa ggagaaatga tgcatactta tttgcaaaag aaaattgaaa
    22381 atatcaaaac aaccctaggt gaaatgtcag gtggttaccg tcgtatggtt gcggctatgg
    22441 ctgatttagg attttcagga actatgaagg ctatctggga tgacctcttt gcccatcgta
    22501 gttttgccca gtggatttat ttgctggttt taggaagttt tcctctctgg ctggagttgg
    22561 tttacgaaca tcgtattgtt gactggattg ggatgatttg tagcttgaca gggattatct
    22621 gtgtaatctt tgtatcggaa ggtcgagcaa gtaattatct ttttggcttg attaactctg
    22681 ttatttacct tattttggcc ctacagaaag gcttttatgg tgaggtgctg acgacacttt
    22741 acttcacagt catgcagcca attggacttc tagtttggat ttatcaggca cagtttaaga
    22801 aggaaaagca ggagtttgtc gcgcgtaaac tggacggcaa gggctggaca aagtatcttt
    22861 ccattagtgt gctttggtgg ttggcctttg gcttcattta tcagtctatt ggtgccaatc
    22921 gtccctatcg tgattcaatc acagatgcaa ccaatggggt agggcaaatc ctcatgacag
    22981 ctgtttaccg tgaacagtgg atattctggg cggctaccaa tgttttttca atctatctct
    23041 ggtggggaga aagcctgcaa attcaaggga aatatctaat ttatctcatt aacagtctag
    23101 ttggttggta tcaatggagc aaggcagcta agcagaatac tgatttactt aactaggaaa
    23161 agatgtttga aagtgctgtt ttgagatttc gattaaaaca gatatagttg ataatcaagg
    23221 atttatagta tgaaaaagag gatcggcggg tcctcttttg ttgttgaaaa gataaaaaac
    23281 tcagtaacct agaaataaga caactgagct ttactctata ttcaattttt aggaatgaga
    23341 aggtctagat aaaattggac aacttcctgg tctgtgaaat cttgaccttt tttgagccac
    23401 caggtcaatg tctcgataaa gttggacatg accaagtgtt ggaggtaaga agtaggtaga
    23461 cttgggtgag tttgcttcaa atcagcagcc agcatgggat agacgtggtg ctcaagctct
    23521 ctctgtagtt gacggaggaa atagtcattt ttggagaata gtagactggc gatatggtct
    23581 tggtttttct gaaaatgcag aaagaggtgg gcgaggtagt cttcggttga aatggcttgc
    23641 tctctttcaa aaagatgatg gaagaggtag cggcagagct gatccagaag aagctcttta
    23701 ctctcatagt gacagtaaaa ggtggatcgt cccacatctg cgagatcaat gatatcctga
    23761 acagtagtgg cctcgtagcc cttagcattc aaaagttgta taaaagcttg atagatggct
    23821 tttttggttt tgctgatacg gcggtcaatg ttagtcatat ggacacttaa ggcaaattgt
    23881 tcagaactga ataaagctga cgttttgctt ctatcctttc tttgagtttt agtggataat
    23941 gataatgaac aagatgttca taaatctatt ataacaaagg aatgagaaat atgaaggcaa
    24001 aatatgctgt ttgggtggct tttttcttaa atttgactta tgccattgtt gagtttattg
    24061 caggtggagt atttggttct agcgctgttc ttgctgactc tgtgcatgac ttgggagatg
    24121 cgattgcaat tggaatatca gcttttctag aaacaatctc caatcgtgaa gaagacaatc
    24181 agtacacctt gggctataag cggtttagcc tgctaggagc cttggtaaca gctgtgattc
    24241 tcgtaacggg ctctgttcta gtcattttgg aaaatgtcac gaagattttg catccgcaac
    24301 cagtcaatga tgaggggatt ctctggttag gaattattgc gattactatc aatctgttag
    24361 cgagtctggt ggttggtaag ggaaagacaa agaatgagtc tattctgagt ctgcattttc
    24421 tggaagatac gctagggtgg gtagctgtta tcctgatggc gattgttctt cgatttacgg
    24481 actggtatat cctagatcct cttttgtccc ttgtcatttc tttctttatt ctttcaaaag
    24541 cccttccacg tttttggtct acactcaaga ttttcttgga tgctgtgcca gaaggtcttg
    24601 atatcaagca agtaaagagt ggcctggagc gattggacaa tgtggtcagc cttaatcagc
    24661 ttaatctctg gactatggat gctttggaaa aaaatgccat tgtccatgtt tgtctaaaag
    24721 aaatggaaca tatggaaact tgtaaagagt ctattcgaat tttcctaaaa gattgtggtt
    24781 ttcaaaatat taccattgaa attgatgctg acctagaaac tcaccaaacc cataagcgaa
    24841 aggtgtgtga cttggaacgg agttatgagc atcaacatta gaaaaaagtg aaaaatactt
    24901 gggtactatc ttatttggaa tagagtaatt tctttattat ttaaatattt caaaaattgg
    24961 taagagaaga gcattgtata aacttcagat atatgattgt taatgataaa aatttttcga
    25021 ttagatacaa aatgcttgac ttggagtcaa ctcaaagtta tataataaga taagtgagtt
    25081 agaatagcgt gaattcagtg aatgaaatga gaggaggtta gcgtgtgaat attaaatctg
    25141 ccagtgattt gttgggaatt tcagcggata cgattcggta ttatgaacgg gttggtcttg
    25201 tgccaccgat tactcgtact gctactggga ttcgtgattt tcaagatcag gatatcgaag
    25261 cgctggaatt tattaagtgt tttcgttcgg cgtgtgtctc tgtagatagt ttagttgact
    25321 atatgtcgct ctaccaaaag ggagatgaaa cgagagagga gaggcttggt attttagaag
    25381 aggaaaagca aaaattagag gagcgcttgt ctcagctaca gacagcttta aatcgtttaa
    25441 atctcaaaat taaactttat aaggaaggaa aattttaaat gaaatcagca gtatatacaa
    25501 aggcaggtca ggttggactt gctagcattg aacgtccgca aataatagaa gcggatgatg
    25561 tgattattcg tgtggttcgt gcgtgcgttt gtggttcaga tttatggagg taccgtaatc
    25621 cagaaacgaa agctggacac aaaaatagtg gacacgaagc gattgggatt gttgaagaag
    25681 ctggggaagc cattacgacg gtgaaaccag gtgattttgt gattgtccct tttacacatg
    25741 gatgtggtga gtgtgatgcc tgtcttgctg gatttgacgg ttcttgcgac aatcatattg
    25801 gcaataattt ggggggtgat tttcaggcag aatatattcg cttccactat gcaaactggg
    25861 cgctggttaa aatccctggt caaccttctg actatacaga agggatgctc aagtcccttt
    25921 tgactcttgc agatgtcatg ccgacaggct atcatgcggc gcgtgttgca aatgttcaaa
    25981 aaggggacaa ggttgttgtt atcggtgatg gggctgttgg tcaatgtgct gtcatcgcgg
    26041 ctaagatgcg tggagcatca caaattatcc ttatgagccg tcatgaagac cgtcaaaaga
    26101 tggctatgga gtcaggtgcg acagctgttg ttgcagaacg tggtcaagaa ggaattacca
    26161 aggtgcgtga aatcctcggt ggaggagcag acgcagcact tgaatgtgtt ggtacggagg
    26221 ctgctataga acaggcgcta ggtgttcttc ataatggagg gcgtatgggc tttgtaggag
    26281 tcccacacta taataatcgt gctcttggtt cgacatttat gcaaaatatc tctgtagcag
    26341 gtggggcagc ttctgctaca acatacgata agcaattttt actaaaagcc gtccttgatg
    26401 gtgatatcaa tccaggtcgc gtctttactt caagttataa actggaagat atcgaccaag
    26461 cctacaaaga tatggatgaa cgtaagacaa ttaagtctat gattgtaatc gaataaaaaa
    26521 cgaataggag ttttagaact ctattcgttt tttatgttat cctattcttg atttagggta
    26581 ctttctctta atgtcagtct ggttcccagc atggtcaggc taggaatttt gcgaccgtgg
    26641 aggacttcct tgttaagaat atccatacct gctcgcccca tttcttcagt ataaactgta
    26701 atactagaga ggggaggata gacctgtttg gtcagactgg tgtcgttgaa tgaaatgagg
    26761 ctgacgcgat ctggcaggct gattccagct tcttggaggg cacggagggc accgatagct
    26821 aaactatcgc tggctgcgaa aaatgctggc ggaagttggt ctcccaagct ctgaatggcc
    26881 tccttcatta agtcatagcc agactgggca gtaaatcttc cttgaaagac cagttcatca
    26941 tgatagattc ccctcgcttg actgtagttt ttgaagtttt ctagacgctt gtcctgaatg
    27001 atttcttctt ggtcagttgt ttcttcaagg cctgttagaa tcccgatacg gtccatccct
    27061 tgactgagga aataatcgac aacctgtttc atggcagtgt aaaagtccgt aataatacag
    27121 gtatgtccca gggaaagtgt atcgctgtct agaaatacaa gaggcttttg gtattcttca
    27181 aaggcagaaa tctgagctcg actaaacttt ccgatgcaga gaatcccaat cacttcctcg
    27241 cttagggtaa aagggtggtc attaaaatag cgcaagatat cgtagtccaa ctcttgggct
    27301 cttttttcta ttcctaggcg aatctggtag tagtagaggt cgtccagctc cccttgttcg
    27361 ctgacccatt gaataatggc aatcttttgc ttgggtttat gggactcgcc tgtcttgagg
    27421 tgcttggtgt agcccagctc ttcagcaacg gttaaaatac ggtgtctggt ttcttctgta
    27481 acagataggc tctggtcgcg gttgaggacg cgggatacgg tcgcgataga gacagaggct
    27541 agctgtgcaa tgtcttttaa ggtagccata aatcctcctt gattaggtta gtatatcata
    27601 tttttctgct ttttactgat agtttagtaa aattttagta aaaaggattg accttgggaa
    27661 atcccttgga tacaatagaa gaaaacgatt acacgttaag gtggcttaac ggacagtcaa
    27721 aggagaaatc atatgacaca acatcttact gctgaaactc ttcgcaaaga ctttcttgct
    27781 gtttttggtc aagaagcaga ccaaactttc ttttcaccag gccgtattaa tttgattggt
    27841 gaacacacag actacaacgg tgggcacgtt tttccagctg ctatttcctt aggaacttat
    27901 ggtgcagctc gcaagcgtga tgaccaagtc ttgcgtttct actcagctaa ctttgaggac
    27961 aagggcatta tcgaagtgcc tctcgctgac ctcaagtttg aaaaagagca caactggacc
    28021 aattatccaa aaggtgtcct tcatttcttg caagaagctg ggcacgtgat tgacaaaggt
    28081 tttgattttt atgtttatgg gaatatccca aatggtgctg gtttgtcttc ttcagcatcc
    28141 cttgaactct tgacaggggt agtggcagag catctctttg atttaaaatt agagcgtctc
    28201 gatttggtta aaatcggaaa acaaacagaa aacaacttta tcggagtcaa ctctggtatc
    28261 atggaccagt ttgctattgg tatgggggcc gatcaacgtg ctatttacct tgataccaac
    28321 accttagagt atgatttggt gccacttgat ttgaaggaca atgtcgttgt tatcatgaac
    28381 accaacaaac gccgtgaatt ggcggactct aaatacaatg aacgtcgtgc tgagtgtgaa
    28441 aaagcagtgg aagaattgca agttgccttg gatattcaga ccctgggtga attggacgag
    28501 tgggccgttg accaatatag ctatttgatt aaagatgaaa atcgtttgaa acgtgctcgc
    28561 catgctgtgc ttgaaaacca acgtaccctt aaagctcaag cagcccttca agcaggtgat
    28621 ttggaaacat ttggtcgttt gatgaatgcg tcacacgttt ctctggagca tgattatgaa
    28681 gtaactggtt tggaattgga tactcttgtt cacacagctt gggcacaaga aggagttctc
    28741 ggtgctcgta tgacaggggc tggttttggt ggctgtgcca ttgccttggt tcaaaaagat
    28801 actgttgagg cctttaagga agctgtaggc aaacactacg aggaagtagt tggatacgct
    28861 ccaagcttct atatcgctga agttgcaggt ggcactcgcg tccttgacta gtcaaaagga
    28921 ggctctatag tgatcttagt agataaattt gtaacacatg tcatttctga aagttcattt
    28981 gaggaaatgg atcgaatcta cctgaccaat cgtgttttgg cacgagtggg agaaggtgtt
    29041 ttggaagttg agaccaatct ggataaattg attgacctca aggaccagct agttgaggaa
    29101 gccgttcgat tagagacgat tgaggatagt cagactgcgc gtgaaatcct tggtactgaa
    29161 ttgatggact tggtgactcc ttgtccgagt caggtcaatc gtgacttctg ggaagcctat
    29221 gcctactctc ctgagcaagc gatagaggat ttttatcaac tcagtcagaa aaatgactac
    29281 atcaaactca aggccattgc taaaaatatc gcttatcgtg ttccatctga ttatggagaa
    29341 cttgaaatta ccatcaatct ctctaagcct gaaaaggatc cgaaagagat tgcggcagcc
    29401 aagttggtgc aagctagtaa ttatcctcag tgtcaacttt gtttagagaa tgagggttac
    29461 catggtcggg tcaaccaccc agctcgcacc aaccaccgga ttatccgttt tgaaatggtt
    29521 ggtcaggaat ggggtttcca gtattcgccc tatgcttact ttaatgagca ttgtatcttt
    29581 ttagatggcc agcatcgtcc catggccatt agtcgtcaga gttttgaacg tctgttggct
    29641 atcgtagagc agtttccagg atattttgct ggatctaatg ccgacctgcc gattgtgggg
    29701 ggctctattc taactcatga tcattatcag ggaggccgtc acgtatttcc tatggaattg
    29761 gctcccttgc aaaagacctt ccgatttgct ggttttgagc aggtcaaggc tggaattatc
    29821 aagtggccaa tgtctgtcct acgcttgact tcggattcca aagaggattt gattaactta
    29881 gctgataaga ttttccagga atggcgtcag tattcagatt ctagtgtgca gattttagca
    29941 gagacagacg ggacaccgca tcacaccatc acaccaatag cgcgtaagcg tgatggacag
    30001 tttgagttgg acttggtctt gcgagacaat cagacttcag cagagcatcc tgatggcatc
    30061 tatcatcccc acaaggatgt ccaacatatc aagaaggaaa atatcggctt gattgaggtc
    30121 atgggcttgg caatcttgcc accacgtctg aaagaagaag tggagcaagt cgctagctat
    30181 cttgtaggag aagctgttac agttgccgat tatcatcagg agtgggcaga ccaactcaaa
    30241 tcccaacatc cagacctaac ggataaagaa aaagcccttg caatcgtcaa ggactctgtg
    30301 ggggctatct ttgtgcgtgt acttgaggat gcaggagtct acaagcagac agagcaggga
    30361 caggcggcct ttatgcgctt tgtggaacag gtcggaattt cactagacta ggagctttct
    30421 ccttgcacag tcctataaaa agtagtatca tagtgtcgtt tgaaatatca ggaggaaacg
    30481 atgaaagact ttcattttga cgctatatct gcctttgaaa attacgaaat tgaaaaaatg
    30541 agagatggtc acgttgtggt gacgaccaaa gtagtgaact cgtcgctcaa ctactatggc
    30601 aatgcccatg gtggctatct attcacgcta tgtgaccaaa tcagtggttt ggtggttatc
    30661 tcgctgggac ttgatggggt gacactccaa tcctctatca actaccttaa ggcaggaaaa
    30721 ctcgacgatg tattgaccat taaaggaaaa tgtgtccatc aaggtcgtac aacctgtgtg
    30781 atggatgtgg atatcaccaa tcaagagggc agaaatgtct gtaaagcaac ctttaccatg
    30841 tttgtcacag gccagcggtc agaagaaaga cgggtaagta tataaagaaa ggtgagtggg
    30901 ataaatttat ctcactcatt tttaatttat gagatatgtt agaaggaaat ataaaagaga
    30961 gtgctgaaat acaaatgtaa taggtacata tatgtggtat actagttata gtagatttta
    31021 attgcttata tcgagtgagt tctcatatta gtgaggaatt tttaaatgga attacacttt
    31081 aatttagaat tagtagaaac ctataaaagt aatagccaaa aggcacgtat cttaacggag
    31141 gattgggttt atcgtcagtc ttattgccct aattgtggga acaatccctt aaatcatttt
    31201 gaaaataatc ggcctgtagc agatttttac tgtaatcatt gtagtgagga gtttgaacta
    31261 aagagcaaaa aaggaaattt ttcatcaaca atcaatgatg gtgcttatgc aacgatgatg
    31321 aagcgtgtgc aggcagataa taatcctaat ttcttttttt taacttacac aaaaaatttt
    31381 gaggtaaata actttcttgt ccttccgaag caatttgtta caccgaaatc gattattcaa
    31441 agaaaaccac ttgcaccaac tgctagacga gcaggttgga ttggttgtaa cattgattta
    31501 tcacaagtac cttctaaagg aaggatattt cttgtgcaag atggacaagt tagagatcca
    31561 gaaaaagtta caaaagaatt taagcaaggt ttatttttaa ggaagagctc tctgtcatca
    31621 agaggttgga caatagaaat tctaaattgt atagataaga tagagggttc agaatttacc
    31681 cttgaagata tgtatcgttt tgaaagtgac ctaaaaaata tctttgttaa gaacaatcat
    31741 atcaaagaaa agattaggca acagcttcaa atattaagag acaaagaaat aatagaattt
    31801 aaaggtagag gaaagtatcg gaaattatga aaacgaaaca acttgttgca tcagaagagg
    31861 tgtatgattt cttaaaagtc atctggcctg attatgaaac tgaaagccgt tacgataacc
    31921 taagtttaat cgtctgtacc ttatcagatc ccgattgtgt gagatggtta tctgaaaata
    31981 tgaaatttgg tgacgaaaaa caactagctt tgatgaagga aaaatatggg tgggaagtag
    32041 gagataaatt gccagagtgg ctacatagct cctatcatag attattgtta ataggtgaat
    32101 tattggaaag caatctaaaa ctgaaaaagt atacagtaga aattacagaa actttatcac
    32161 gtttagtaag tatagaggct gaaaatccag atgaagccga acgacttgta agagaaaagt
    32221 ataagagttg tgaaattgtt cttgatgcag atgattttca ggactatgac actagcatat
    32281 atgaataggt agatgttttt attttgtcac acaaaaaaga ggctcgcacc tctttttctt
    32341 atttcttttt atgatttaat acggcattga ggacaatagc gagtaggctg gctacgacga
    32401 ttccgtttga gaagaacatt tggaaggctg tcggcatgct gacaaagaga ttactgttgt
    32461 tgagaccgac acctgcagcg attgaaacag ctgcgataag gaagttgtgt tcattgttag
    32521 caaagtcaac tcgggctagg atttgcatcc cttgaataga tacaaaacca aacatcacca
    32581 gcatggcacc gccgaggacg gagcttggaa tgatttgggc aagggcacca aacttaggaa
    32641 gcagtccaag gagaaccagg aaaccagctg cgtagtagat tggcaggcgt tttttgatgc
    32701 ctgacaattt aaccaaacca acgttttgtg aaaatccggt gtaagggaag gtgttaaaga
    32761 ttcctccgag aagtacggcc aaaccttctg cgcggtaacc gttgcgaagg cgcgtgctgt
    32821 cgattggatc ttttgtgata tcagacaagg ctagataaac accagttgac tcaaccatag
    32881 acaccgttgc gatgatacac atcatgacaa tagatgagat ttcaaaggtt ggcatcccaa
    32941 agtagagtgg agttgggaca tggacaagtg gagctaccgc aacaggagag aagtccacca
    33001 agcccatagt agcagcaatg gcagttccaa caaccagacc aatcaaaata gagatagact
    33061 tgataaatcc tttggtaaag atgttgatca agaggataat cagaacagta atagctgcaa
    33121 gcaagagact ttgaccagtt ggctctggaa cgttatttcc catatttcca atagcgacag
    33181 ggatcaaggt taaaccaatc gtggtaataa cagatcctgt tacgatagat gggaagagat
    33241 tggctacttt tgagaagatg cctgaaacaa gaaccacgta aatcccagat gcgataaggg
    33301 caccaaacat agcgccacta ccatggcttt gcccaatcat aatcaaggga gcgaccgact
    33361 ggaatgcaac tccaagaacg actgggagtc caatcccaaa gtatttgttg agttggagtt
    33421 ggaggaaggt tgccacccca cacatgaaga tatctgtaga aatcaggtag gtcaactgct
    33481 cagctgaata gccaagggct gtcgcaatca tgatgggaac caggatagat cctgagtaca
    33541 tggctagtaa gtgctgcaag cccagaacgg ctgcttgcga gtgtttttct tgagtttgca
    33601 ttagagatct gcctccttaa atacgacctg accattttca aaacgatcca aacgagcaag
    33661 tgataggaca gggtagcctg ctttttcaag caaatcacga ccatcttgga aggatttctc
    33721 aatcacgata ccgatagctt ggactgtggc accggcctgt tcgatgattt gaatcaagcc
    33781 tttagcagct tggccattag caaggaaatc gtcgataatc aaaaccttgt cttctggtga
    33841 gaggaatttt ccagcgatag aaacggtgct ggtcacctgc ttggtaaagg agtagacttg
    33901 agcagttaag atgccttcgt tcatggtgat gttcttagct tttttggcga aaatcatggg
    33961 aacgtttaag gcttcagctg taaaaacggc tggggcaata cccgacgctt caatggttac
    34021 gaccttggta atgccagcag cagcaaattt ttccgcaaaa accttaccaa tctctcgcat
    34081 caagctaaag tcaacttggt gggttaaaaa ggaatctacc ttgaggatgt tatcacccaa
    34141 gatatgccca tccttgagga tgcgctcttc taataatttc ataagacctc ctaaagtcta
    34201 aaagttaatt tacttgttgt ttaaatgttt ctatagtgat cccttttgct aatactatat
    34261 atttgataaa actattacga gcgaagcgag tcttatcaaa tatttcccgt tgtagtggta
    34321 tcatagacaa taatcttgtt attgtctatg acgggatttt tgagagtaaa atagttcggg
    34381 gaactatttt agcctaagcc tagaaatgaa agagctaggg gctcaaaaat tagggatgaa
    34441 attccctgga ttcctgaaat tattcacagg ataatttcac ctcccgtccg cactaattaa
    34501 gggaaatatt aaaaatattc aaaaaagacc tacttaatct ctaagtaagt cccccaaata
    34561 ggcatggcaa aaacggccat atctcactgc tgacttactt attgttaggt gttccggcac
    34621 cttgtagaaa cgtcgtgcca attcacgaca taaacaagta aaacgatatt caattttaaa
    34681 taggcttgaa ccaatgtttt tattttacac taaataactt tagaaatcaa ctattttgtt
    34741 agtattttgg tttaaaaaac gaacaaacag gagaggggat gaacaaaaac tccattgtaa
    34801 gctaacattt atactaaatg aaaatcaaag agcaaactag gaagctatcc acaacctcaa
    34861 aacactgttt tgaggttgtg gatagaattg acagagtcag tatcatatac ctacggtaag
    34921 gcgacgttga cgtggcttga agagattttc gaagagtatt agaagatttt tccatcataa
    34981 aaggcatact atcaagtttt taaacacctt acaatatgcc tttttctaac tttaaagact
    35041 tttcccaatt tttattattc tactcgctaa atcttaaaaa atagccattt ggatccaaaa
    35101 ctgcaaattt atgaggatag atatagggat cactgacacg aaactttctt ttggtcaagg
    35161 ggcgataaat aggatagttt gccttcatca ctctttaata gagttttgaa acatccttta
    35221 tgccaaagga gagattgact ccacgaccaa atggataggt cagttcagct agttgatcct
    35281 ttgttccctc ctctaacatt agttgacact cttcaagaga aagaaagaga aagttttctt
    35341 ctggacgttg gtattcaatc ctaaaaccca gtaaaccaca gtagaaggac cgggactgtt
    35401 cgatattcga tacaagcaac tcgggaatga ccgcattgta gtccatatag aaaatcctta
    35461 caagtcaatt tccaagacaa tcggtgtatg gtcttggcga gcacctgagt caatcatatc
    35521 agatttagtg accttgtcag cgatacggtt acttgtgagc cagtagtcga ttctccagcc
    35581 tgtattgttg attttagaag ttttgctgcg ttgtgcccac caagtgtagc gttcaggaac
    35641 atcgccatga acatggcgga aggtgtctgt aaatccagtt gccaaaaggt tggtaaatcc
    35701 agcacgttcc tcgtcagtaa atccaggtga acggcggttg ctagcaggat ttgcaaggtc
    35761 gatttcattg tgggctacgt tgtagtcacc ggtcgcaagg actggttttt ctttgtctag
    35821 ttcagccaaa tactccgcat atttaacatc ccagacttgg cgttcttcca agcgtttgag
    35881 accgtcacca gcgtttggag tgtaaacttg ggttacgaaa aatgcatcaa attctagagt
    35941 gatgatacgg ccttccaagt ccatggtaga aggggcaccg atttctggga agctgatcgt
    36001 aggcgtaagt tctttcttat aaaggaacat ggttccagca tagcctttac gggcaggctc
    36061 ttgggaagag cgccacgtgt tttcgtagcc tgggaagagt tcttctaaaa tttccacgtg
    36121 tttctttgta ggtcctttgg cagaaagctt ggtttcttgg atagcaataa tatcagcatt
    36181 ttcagcgacc aaggtttgta ggacttcttg ggacaattta gcacgagctg agtcactagt
    36241 tagggcagcg tttagggaat caatattcca tgagataagt ttcataaagt tacctttttc
    36301 attcagatta tagattttat tataccaaaa aaagatctat ttccccaacg tatggtttga
    36361 aaaattactc tctttcgttt ataattaaga atgattttat gaaagggagt gaaaatacat
    36421 gaaattctac ttttatgact atgtactcag ccaaatcggt cagcaaaatg gtattatggt
    36481 tggctttggg attgttctat tagctgtgac agtttttttt gctttcaagg cataccatga
    36541 taaaaaagga accaaatttc gtgagttggt catgatttca gctctggcct tatttagctc
    36601 tgcttttggt cagcatcacg acttatcaaa acaatcaagt ttctaacaat aaatttcaaa
    36661 cttcacttca tttcatcgag gttgtttcca aagatttggg agtagacaag tcagaagtct
    36721 atgttaatac ttccacaaac acagatggcg cacttatcaa ggtgggagat cgctattatc
    36781 gtgccctaaa cggaagtgag ccagacaagt acctgttaga gaaagtcgaa ttgtataaga
    36841 cagacgcaat tgaactggtg gatgtgaaca aatgacactt aattatatcg aaattttaat
    36901 caaactggtc ttgactctca aatagctcaa caacaatgtt cactttgtga aacgtttgat
    36961 tgatggtaag ccaactctcc ttatcaaaaa tgggaatatt gacccataag cctgtcgttc
    37021 agttggtttg tctgcatcgg atgtagccct caaacttcgt agccaaggga ttttccagat
    37081 gaagcaagtc aaacgagctg tgcaagagca aaatgggcaa ctcatcgttg tgcaaatggg
    37141 agatgaaaat cctaagtatc cagttgtgac tgacggtgtg attcaagtag atgtcttgga
    37201 atcgattggt cgtagcgaag agtggttgct tgataacctc agtaaacaag ggcatgacaa
    37261 tgtagccaat atctttattg ctgaatatga caagggtgct gttacagtcg taacttatga
    37321 aaaagaaaaa cctggggtct tgtactcttc gaaaatctct tcaaaccgcg tcaacgtcgc
    37381 cttgccgtat gtaggttact gacttcctca gttctatcta caacctcaaa gcagtgcttt
    37441 gagcagcctg cggctagttt cctagtttgc tctttgattt tcattgagta ttggcctcag
    37501 gtttccattt gcaatcagaa agggatttta tgtccattat tcaaaaactc tggtggtttt
    37561 tcaagttaga aaaacgccgt tatctagtcg gaattgtggc cctggtcttg gtttccgtcc
    37621 tcaatctcat tcctcctatg gttatggggc gggtcattga tgccatcaca tcggggcaat
    37681 taacccagca ggacctcctt cttagcctat tttacttgct acttgcagcc tttggtatgt
    37741 actatttgcg ctatgtgtgg cgtatgtata tccttgggac ctcttattgc ttgggacaga
    37801 tcatgcggtc tcgcttgttt aagcatttca caaaaatgtc gtcagccttt tatcaaacct
    37861 atcggacggg tgatctgatg gcacacgcaa ccaatgatat caatgccttg actcgtttag
    37921 caggtggcgg tgtcatgtct gcggtggatg cctctatcac ggctctggtg actttgttga
    37981 ccatgctctt tagcatctca tggcagatga ctcttgttgc cattctcccc ctacctttca
    38041 tggcctatac gactagtcgc ctagggagaa agactcataa ggcctttggc gaatcccaag
    38101 ctgctttttc tgaactcaat aacaaggtac aggagtccgt atcaggtatc aaagtgacca
    38161 agtctttcgg ttatcaggca gacgagttga agtcttttca ggcagtcaat gaattaacct
    38221 tccaaaagaa cctgcaaacc atgaaatatg atagtctctt tgaccctatg gttctcttgt
    38281 ttgttggttc gtcctatgtt ttaacgcttt tggttggctc cttgatggtt caggaagggc
    38341 agattacagt tgggaatcta gtcaccttta tcagctattt ggatatgctg gtctggcctc
    38401 ttatggccat cggcttcctc tttaatacta ctcagcgagg gaaggtttct taccagcgga
    38461 ttgaaaatct tttgtctcag gaatcttctg tacaagaccc tgagtttcct ctggatggta
    38521 ttgaaaatgg gcgtttggag tatgccattg acagctttgc ttttgaaaat gaggaaacac
    38581 tgacggatat tcactttagt ttggcaaaag ggcaaacact gggcttggtt gggcagacag
    38641 gctctgggaa aacgtcctta atcaagctcc tcttgcgtga atacgatgtg gataagggta
    38701 ctatttatct aaacggtcac gatattcggg actatcgtct gacagacctt cgcagtctca
    38761 tgggctatgt tcctcaggac cagtttcttt ttgcgacttc aatcctagac aatatccgct
    38821 ttggcaatcc taacttgccc ctttcagcgg tcgaggaagc tactaagcta gcccgagttt
    38881 accaagatat tgtagaaatg cctcaaggat ttgatacgct gattggtgaa aaaggagtca
    38941 gtctttctgg tggtcaaaag caacggttgg ctatgagtcg ggctatgatt ttaaaccctg
    39001 atatcttgat tttggatgat tccttatccg ccgtagatgc caagacagag tatgcgatta
    39061 tcgacaacct caaggagacg cgaaaggaca agacaaccat tatcactgcc catcgcctca
    39121 gtgctgttgt ccatgcagat tttattttag ttctacaaaa tggtcaaatt atcgaacgag
    39181 gcaggcacga agacttgcta gcgatggatg gctggtatgc ccaaacctac cagtctcagc
    39241 agttggaaat gaaaggagaa gaagatgcag aataaacaag aacaatgggt tgtattgaat
    39301 cgcttgatgt cttatctcaa gccttataga ctcctgacct ttttggcact cagttttctc
    39361 ctagcgacga cggtcattaa aagtgtcata cccctcgtgg cttcccactt tatcgaccag
    39421 tatctcagca atcttaacca actagccgtt accgttttgc tggtctacta tggtctctac
    39481 atcctacaaa ctgtagttca gtatgtaggc aatcttctct ttgcgcgcgt gtcttacagt
    39541 attgttaggg atattcgtcg agatgccttt gccaatatgg agaaactggg catgtcttac
    39601 tttgacaaga cgccagcagg ttctatcgtt tctcgtttga ccaacgatac cgagacgatt
    39661 agtgatatgt tttctgggat tttatccagc tttatctcag cagtttttat ctttctgaca
    39721 accctttata ccatgttggt gctggatttt cgtttgacgg ctttagtctt gctctttctt
    39781 cctttgattt tccttttggt caatctctat cgaaaaaagt cagtgaaaat catcgagaaa
    39841 accagaagtc tcttgtcaga tatcaatagt aagctggcag agaatatcga gggaatcagg
    39901 attattcagg cctttaatca agagaagcgc ctgcaggcag aatttgatga aatcaaccaa
    39961 gaacacttgg tctatgccaa ccgttctgta gccttggatg ccctcttttt gagacctgcc
    40021 atgagtttgc tgaaacttct aggctatgca gtcttgatgg cctactttgg ctaccgtggt
    40081 ttttctatcg ggataacggt cgggaccatg tatgccttta tccagtacat caaccgcctt
    40141 tttgacccct tgattgaggt gacgcaaaac ttttcaactc tgcaaacggc tatggtttct
    40201 gcaggtcgtg tctttgccct gatagacgag aggacctatg aacctcttca agaaaatggg
    40261 caagccaaag tccaagaagg caatatccgt tttgaacatg tgtgtttctc atatgacggt
    40321 aaacatccga ttctggatga catttctttc tctgttaata agggtgaaac cattgccttt
    40381 gtaggtcata caggttcagg gaaatcgtct attatcaatg tcctcatgcg cttttatgaa
    40441 ttccagtcag ggagagttct cttggatgat gtggatatca gggatttcag tcaagaagag
    40501 ctgagaaaaa acatcggttt ggtcttgcag gaacccttcc tctatcatgg aactattaag
    40561 tccaatatcg ccatgtacca agaaaccagt gatgagcagg ttcaggctgc ggcagccttt
    40621 gtggatgcag attcctttat tcaagaactt cctcaggggt acgactcccc tgtttccgag
    40681 cgtggttcga gcttctctac tgggcaacgc cagcttcttg cctttgctag aacagtcgcc
    40741 agccagccta aaatcctgat tttggatgaa gcgacagcca atattgactc tgaaacagaa
    40801 agcttggttc aagcttctct ggcgaagatg agacagggcc gaacaactat tgctatagct
    40861 caccgccttt ctactattca agatgccaac tgcatctatg tcttggataa gggacgcatt
    40921 atcgagagtg gaacccatga ggaactcttg gctctgggag gaacctatca caagatgtat
    40981 agtttgcagg caggggccat ggcctatact ctttgaaaat ctctttaaac catgtcagct
    41041 ttatctgcaa tctcaaagct gtactttgat tttcattgag tactagaagg aaatccttca
    41101 aattacagat ttctttcacc gcattttcca ttttgtggta taatgaaaaa tgttgacaaa
    41161 tagtataata aaaacaaagg agaaaccgca tgctgaaatg ggaagacttg cctgtggaaa
    41221 tgaaatcaag cgaggttgag ttttactacc aacttgtctc taaaaggaag gggtcgctga
    41281 ttttcaagcg ttgcttggcc ttggttttgg ccttggtctt actggttctg acctctccca
    41341 tctttctcat cttgagcatt tggatcaagt tggatagcaa ggggccagtg atttacaagc
    41401 aagagcgtgt gactcagtac aaccgtcggt tcaagatttg gaagttccgt accatggtga
    41461 tggatgcgga taaaaaagga agtctggtga cttctgctaa cgatagccgc attaccaagg
    41521 ttggaaattt catccgacgt gttcgtttgg acgaactgcc tcagttggtc aatgtcctta
    41581 aaggtgagat gtcctttgtc ggtacacgac ctgaagtgcc acgttataca gagcagtaca
    41641 gccctgaaat gctggcaacc ttgctcttgc cagcagggat tacctctcca gccagcatca
    41701 actacaagga tgaggacacc atcatcagtc aaatgacgga gaaaggtctg tcagctgatc
    41761 aggcctatgt ggagcatgtt cttcctgaaa agatgcgcta taacctcgcc tatctccgag
    41821 agtttagttt ccttggggac atcaaaatca tgtttcaaac cgtgtttgag gtactaaaat
    41881 aaagtagtca taagaaaatg agtacagata aaaggagaaa agcaatgcca aattacaata
    41941 ttccattttc accgcctgat atcacagaag cagaaattgc tgaagtagcg gataccctgc
    42001 gttctggttg gatcacaaca ggtcctaaaa caaaagaact ggagcgccgc ttgtctcttt
    42061 acacacagac acctaagact gtctgtctca actctgcgac agccgctctg gagttgattt
    42121 tacacgtttt ggaagtggga cctggtgatg aagtcatcgt tccagccatg acctataccg
    42181 cttcatgtag tgtcataact cacgtgggag caactcctgt catggtggat atccaagcag
    42241 atacgcttga gatggactat gacctgctgg agcaagccat cactgaaaag acaaaggtga
    42301 ttatcccagt agagcttgca gggattgttt gtgactatga ccgtttgttc caagttgtgg
    42361 aaaacaaacg tgacttgttt gttgcttcaa gcaagtggca aaaggccttt aaccgtattg
    42421 tcattgtctc tgatagtgcc cacgctttgg gatctactta taaaggacaa ccttctggtt
    42481 ctatcgctga ctttacttcc ttctcattcc atgccgttaa aaactttaca acggccgaag
    42541 gtggaagtgc gacttggaag gccaatccag cgattgatga cgaagagatg tacaaggaat
    42601 tccaaattct ttcccttcac gggcaaactg aggacgctct tgccaagatg caactggggt
    42661 catgggaata cgatatcgtt acaccagcct ataagtgcaa catgaccgat atcatggctt
    42721 cacttggttt ggtacaattg gaccgctatc caagtttgtt gcaacgccgt aaggacattg
    42781 tggaccgcta tgatagtggt tttgcaggtt ctcgcatcca tcctttggca cacaagactg
    42841 aaactgtcga atcttcacgc cacctctaca tcacccgtgt agaaggagca agcctagaag
    42901 aacgcaacct catcatccaa gaattggcta aagcaggaat tgcaagtaat gttcactaca
    42961 aaccgcttcc tctcttgaca gcctataaga atcttggatt tgatatgacg aactatccta
    43021 aggcctatgc cttctttgag aacgaaatta ccctccctct tcatactaaa ttaagcgatg
    43081 aagaagtaga ctatatcatt gagactttca aaacagtttc tgaaaaagtg ctaactttat
    43141 caaaaaaatg acaaactaca gtcaagcgaa agtgatcctg tccctaaaaa gtctaattga
    43201 gtgtaaaaac tgttgttttc aattgataat agtttacacc tgtagttgag gcccctctct
    43261 cctcagagag agaattttta taggattttc ctttcttgtg ggagtcccgt ggtttgaaat
    43321 aagatgtgag caatttggtg tagcatttag aatccttact agacatcatt tagaaaatct
    43381 agtgtcttgt tctagttttt aattcaccct attttttgaa agacgtgagt ttccatgagt
    43441 gagattgtgg aaactcgcgt ctttttttgt tttcagaata ttgttcaaag ttttgtgcct
    43501 gtctttcatg ttctagtcat tcttttgcat gatagaattt atagcatatt gatattataa
    43561 taatacaaac attctatacg tttagtgatg cttgctatac atcattagat ctcccgcgag
    43621 acaatctata aaacacttgt ctacgattac ctatatgccc tattccagta ttttagaagc
    43681 actgcatcta tttttatcga agttaaatct agcttttata gaaggtctat ttaagaaata
    43741 tattgtagtg ttttagtttc aatccgccat atgagcgata ttcaggtaaa tatccctagc
    43801 gaatgcttgt atgacaagat atttgttctt tcatttataa tttacaacat atcaacaaat
    43861 ttaaatatag taaatgggat attttatatt caagctaaga aagatagcat cacttttgaa
    43921 tggaaggcta aagagcaaac taggaagttg gccatagata gctcaaaacc ctgctttgag
    43981 gttgtagata taataaaatg aaataagaat aggacaaatc gatcgggaca gtcaaatcga
    44041 tttctaacaa tgttttagaa gtagaggtgt actattatag tttcagtcta ctatagaact
    44101 gaccaagtca gtaacctaga cttagggcaa ggcggcactg acctagtttg aagagatttc
    44161 cgaagagtat aaattttaat attttcttgt gttattcctt gacaattcaa tttggaaaat
    44221 atatgataaa gataatgaca gcggtgtcat tctatctatt ttaagaaaag taataatcaa
    44281 ttgttaaaaa tagtaaaaaa attggaggtt ctgatgaaat attttgttcc taatgaggta
    44341 ttcagtattc gtaaattaaa ggtggggact tgctcggtac tattggcaat ttcaattttg
    44401 ggaagccaag gtattttatc ggatgaagtt gttactagtt cttcaccgat ggctacaaaa
    44461 gagtcttcta atgcaattac taatgattta gataattcac caactgttaa tcagaatcgt
    44521 tctgctgaaa tgattgcctc taattcaacc actaatggtt tagataattc gttaagtgtt
    44581 aatagtatca gctctaatgg tactattcgt tccaattcac aattagacaa cagaacagtt
    44641 gaatctacag taacatctac taatgaaaat aagagttata aggaagatgt tataagtgac
    44701 agaattatca aaaaagaatt tgaagatact gctttaagtg taaaagatta tggtgcagta
    44761 ggtgatggga ttcatgatga tcgacaagca attcaagatg caatagatgc tgcagctcaa
    44821 gggctaggtg gaggaaatgt atattttcct gaaggaactt atttagtaaa agaaattgtt
    44881 tttttaaaaa gtcatacaca cttagaattg aatgagaaag ctacaattct aaatggtata
    44941 aatattaaga atcacccttc cattgttttt atgacaggtt tatttacgga tgatggtgcg
    45001 caagtagaat ggggcccaac agaagatatt agttattctg gtggtacgat tgatatgaac
    45061 ggtgctttga atgaagaagg aactaaagca aaaaatctac cacttataaa ttcttcaggt
    45121 gcatttgcta ttgggaattc aaataacgta actataaaaa atgtaacatt caaggatagt
    45181 tatcaagggc atgctattca aattgcaggt tcgaaaaatg tattagttga taattctcgt
    45241 tttcttgggc aagccttacc caaaacgatg aaggatgggc aaatcataag taaggagagc
    45301 attcagattg aaccattaac tagaaaaggt tttccttatg ccttgaatga tgatgggaaa
    45361 aaatctgaaa atgtgactat tcaaaattcc tattttggca aaagtgataa atctggggaa
    45421 ttagtaacag caattggcac acactatcaa acattgtcga cacagaaccc ctctaatatt
    45481 aaaattttaa ataatcattt tgataacatg atgtatgcag gtgtacgttt tacaggattc
    45541 actgatgtat taatcaaagg aaatcgcttt gataagaaag ttaaaggaga gagtgtacat
    45601 tatcgagaaa gcggagcagc tttagtaaat gcttatagct ataaaaacac taaagaccta
    45661 ttagatttaa ataaacaggt ggttatcgcc gaaaatatat ttaatattgc cgatcctaaa
    45721 acaaaagcga tacgagttgc aaaagatagt gcagaatatt taggaaaagt atcagatatt
    45781 actgtaacaa aaaatgtaat taataataat tctaaggaaa cagaacaacc aaatattgaa
    45841 ttattacgag ttagtgataa tttagtagtc tcagagaata gtatattcgg gggtaaagaa
    45901 ggaattgtta ttgaggattc aaagggtaaa ataaccgttt taaataacca attttataat
    45961 ttatccggta agtatatatc attcatcaaa tctaatgcaa atgggaaaga acctgttata
    46021 cgtgatagcg atggtaattt caatattgta acggagaatg ggctttacaa aattgtaaca
    46081 aataatttaa gtgataaaaa cgaaaaagaa aaaaacaaag aggaaaaaca atctaattca
    46141 aataatgtaa ttgatagtaa ccagaagaac ggagagttta actcaagtaa agataataga
    46201 caaatgaatg acaagatcga caataaacaa gataataaga cagaagaagt aaactataaa
    46261 atagttggag atggcagaga aactgaaaat catattaata aatctaaaga aatagtagat
    46321 gtaaaacaaa aattaccaaa gactggttcg aacaagatta tggaactatt cttaacagtg
    46381 acaggaattg gtttactttt gacactaaaa gggttgaagt attatggtaa agataaataa
    46441 aatttgttcg atacaaggaa gttccgtaga aaatgaggat attgtaggtt cacaaaatca
    46501 gtatttttgg attattggtg gagctacaga tttatataat tctaaagagg agataggtta
    46561 ttcagtctca gaagttgtac atattttatc agaaagtttg tcggttaatt gtaaagaatc
    46621 aaaaactctt aaacaaatat ttgaaactgc tttactcgag gttaaagatg aaatcggttt
    46681 aaactcatat aaattattgg aggacaacaa aatcgattga agacatgtgt agagatacat
    46741 ggaattggca atcaaaaaat cccaatggct ataattgatg atactgtcta tatgagtgct
    46801 gtattttgaa aaaattactt aaccttttaa tgaaatgcat atttaatgaa agataaattt
    46861 aaaaaaatgt ttgcaagtgc ttgacaaagg aaacggtttc attgtataat gtaaacatga
    46921 tgacaccgat gtcaaatata aaaaagaagg tctttatatg aaaaacttaa caaaaattaa
    46981 gttcaaagag aatggggaat ttaatcattt ccctgggaat acagttgtag caaatcttta
    47041 tactaaacaa gatttgatgg aagttgttga tattattcaa tcacgttata gagaattacc
    47101 atttatcgat aagtttactt taactccaag gaattctatt cacatgactg taattgaatt
    47161 gttgtgccat gaaaatcgtg aaacggagtt ttggagcagt aatcttcctc tagatacacc
    47221 tttacaggaa atacatgatt actttgctaa acaacttgaa atttttccat tgttggatga
    47281 agaaattcat atgcgtataa ctgaaatggg aaaacaaaac atactagttg aacctgcaga
    47341 tgaagcttct gcaaagagat tagaagaaat tcgtacttat gtctcagaaa aagcaggtgt
    47401 ttgtttccct aatcatgata gatatcaatt ccatatttca attgggtatc ttcggattcc
    47461 tctaaccgaa gaggaggaag aagagtttac taaagtcaga gcagaattaa ctgaaatttt
    47521 attagagaag attccaacta ttactgtaaa ccgtattgac tatactgtat ttgaagatat
    47581 gagacaattt gttccatatc acgaaaaatt taaataaaat aataaaaaag gagaacttat
    47641 catgaaaaaa atgaaagttt ggtctactgt acttgcaacg ggagttgctc ttactacact
    47701 tgctgcttgc tctggaggtt caaattctac gactgcttct tcatctgaag aaaaagctga
    47761 taaaagtcaa gaattagtta tctattcgaa ctcagtctca aatggtcgtg gtgattggtt
    47821 aactgctaaa gcaaaagaag ctggttttaa tataaaaatg gttgatatcg ctggcgctca
    47881 attagcagac cgtgttattg ctgagaagaa taatgcagtt gcagatatgg tatttggaat
    47941 tggtgctgtt gactcaaata aaattagaga tcaaaaatta ctagtacagt acaagcctaa
    48001 atggttagat aaaattgatc aatctttatc agataaagat aattattata atcctgtgat
    48061 tgttcaacca ttagttttaa ttggggcgcc tgatgtaaaa gaaatgccta aagactggac
    48121 agagttagct agtaagtata aaggtaaata ttcaatttat ggtcttcaag gtggtacagg
    48181 acgggcaatt ctagcaagta tcttagttcg ttaccttgat gaaaaaggag atttaggcgt
    48241 atcagaaaaa ggatgggaag ttgcaaaaga atactttgca aatgcctata ctcttcaaaa
    48301 aggtgaaagc tcagttgtta aagcgttaga taaagaaagc ccaattcaat acggtatgat
    48361 ctggggttct ggagcgttaa ttggacaaaa agaacaaaat gttgaattca aagttatgac
    48421 tcctgagatt ggtgtaccat ttgtaactga acaaactatg gttttaagca ctagtaaaaa
    48481 acaagcgtta gctaaagaat ttattgattg gtttggtcaa tcagaaattc aagtagaata
    48541 tagtaaaaaa tttggatcta ttcctgcaaa taaagatgcc ctaaaagagt tacctgaaga
    48601 tacgaaaaaa tttgttgatc aagtgaaacc acaaaatatt gactgggaag ctgttggaaa
    48661 gcatttggat gaatgggtag aaaaagctga attagaatac gtaaaataag aaataagagt
    48721 tattttttat gtccaagtct ctctattatt tctaaattta aggaagaaat aaagagaggc
    48781 ttttggtttt agtaatagag ggagttagtg agatgattaa atttgataat attcaaatta
    48841 aatatggtga ttttgttgca attgataatc tgaatttaga tatacatgaa ggggaattct
    48901 ttacatttct tgggccttca ggatgtggta aatcaactac tttgagagca ttggtaggtt
    48961 ttttagatcc atcatcagga agtattgaag ttaatggaac agatgtcact catttggaac
    49021 ctgaaaagcg tggaattggt attgtatttc aatcttatgc gctatttcca actatgactg
    49081 tttttgataa tattgcattt ggtttaaaag ttaagaaggt agctccagat gttattaaag
    49141 ctaaagtatc agcagtggca gcaaaaatta agatctctga tcaacagtta cagcgtaatg
    49201 tatcagaatt atctgggggt caacaacaaa gggtagcatt ggctcgtgct ctggttcttg
    49261 aacctaaaat tctttgtcta gatgaaccat tgtcaaacct tgacgcaaaa ttacgtgtag
    49321 atttgagaaa agagttgaaa agacttcaaa aagagttagg tattactact ttatatgtta
    49381 ctcatgatca agaggaagcc ttgactttat ctgatagaat tgcagtcttt aacaatggat
    49441 acatcgaaca ggtcggtaca ccagtagaga tttatcataa ttctcaaact gaatttgtat
    49501 gtgattttat tggagatatt aatgttttga ccgatgaaat agtccacgaa gtattattga
    49561 aaaatacaag cgttttctta gaggataaaa aaggatacat tcgattagag aaagttcgat
    49621 tcaatcgtga aactgaacaa gattttattc taaaagggac aattattgat gttgagtttt
    49681 ctggagttac aattcactat acaataaaag tttctgaaag tcagattctt aatgtaacaa
    49741 gtattgatag tcaggctgct attagatctg tcggagaaag tgtggaatta tttatcacac
    49801 catcagacgt tctgcaattt taaggaggtg cagtatgcgt cataaattaa atttaaaaga
    49861 ttggcttatt cgtttagggt taatctggtt cttagtaaca tttattattt atccaaactt
    49921 tgatctagta gtgaatgtat ttgtaaaagg aggagaattt tcccttgatg ctgtacatcg
    49981 tgttctaaaa tctcagaggg cacttcagag tattatgaac agttttaagt tagcattttc
    50041 actcattatt acagttaatg tcgtaggtat tctttgtgtt ctatttacag agtactttga
    50101 tattaaaggt gctaaaattt taaaattagg ttatatgacc tctttaattt atggaggagt
    50161 ggttttagcg actggatata aatttgtcta tggtccttat ggattgatta caaaattttt
    50221 acaaaatgtt atcccttctt tagactctaa ctggtttatt gggtatggtg cagtcttatt
    50281 cattatgaca ttttcaggaa ctgctaatca tacattgttt ttaacaaata caattcgaag
    50341 cgttgactat cacactattg aggctgctcg aaatatggga gcaaaaccat ttactgtttt
    50401 ccgaaaagta gtgttaccaa ccttaattcc aactctattt gcacttacta ttatggtttt
    50461 tcttagtggt ttatctgcag tagcagcacc catgattgtt ggtggtaaag aatttcaaac
    50521 tataaatcca atgattatta catttgcagg gatggggaat tctcgtgatt tagctgccct
    50581 acttgcaatt attttaggta ttgcaactac aattttgctt actatcatga ataagataga
    50641 aaaaggtgga aattatattt ctatctctaa gactaaagcg cctcttaaaa aacaaaaaat
    50701 tgcgtctaag ccttggaata tcattgctca cattgtagca tatggattgt tcacagtttt
    50761 catgcttcca ctaattttta tagtattata ctcatttaca gatccagttg caattcaaac
    50821 aggtaactta acattatcaa actttacttt agaaaattat cgcttattct ttagtaatag
    50881 tgcggcattc tctccattct tggtcagctt tatttattct attattgctg cgacaacagc
    50941 aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa cataaatctc gttttgattt
    51001 cttatttgaa tatggtgctc tacttccttg gttactacca agtacacttt tagcagtaag
    51061 tttattattt acttttaatc agccacaatt tcttgtcttg aatcagattt tggtaggtag
    51121 tttggtaatt ctacttattg catatatagt tgtaaaaatc ccattttctt atagaatggt
    51181 acgtgctatt ttatttagtg ttgatgatga gatggaagat gcagcaagaa gtatgggtgc
    51241 ttcacctttt tatactatga tgaaggttat cattccattt attttaccgg ttgttctctc
    51301 tgttattgct ttaaacttta actctttatt aactgacttc gacttatctg tattccttta
    51361 ccatccccta gctcaaccat taggtattac gattcgatct gcaggtgatg aaacagcaac
    51421 atctaatgca caagctctgg tatttgttta tacaattgtt ctgatgatta tttctggaac
    51481 ggtattatac ttcacacaaa gaccggggcg taaagtaagg aaataatcat gacagccact
    51541 agtcttgggt tatcaaatat tgaaatagtt gtcaggattg ttttatcagt agtcattggt
    51601 agtataattg gtttagagag agggagcaaa tcccagcctg caggcatccg aacttatagt
    51661 attgtttgtc tagctgcatg tttgattatg atgacgaatg aatacgtatc ttataaattt
    51721 gggacaggag atcctacacg attaggagct caagttatat caggtgtggg ttttctaggc
    51781 gctggaacga ttcttattac agataaaaag aaaattacag gtctgacaac tgcagcaggc
    51841 atttgggctt cggcaggaat tggattagct attggagtag gtttttatga gggagctctt
    51901 ttagtagcca tttctgtttg gggtgtgata tccatgttcc aaccactaaa aaaatatctg
    51961 caaaatcgtt ctaaaatgat tgaattgtat atagtggtta aatctacaga agcctataat
    52021 cgtgtattgg tttattgtgc agaaaatggt atcagaatga ccgattcaag aacagcgttt
    52081 ggagatgtta attctgacag aattgaatac tttgatgttc cggacaaaaa aatagcgtca
    52141 tttattactc tggaattatc aggtagattt gaacatcttc gactgatgga agaaattgct
    52201 aatattgtag gtgttattta tgtcgaagaa attagctgaa atttagtaaa caaagggaca
    52261 gatgtttttg tccttttgct ttttagatgt tttaatcatc taatttttga gtagattttt
    52321 gctcagaggt ttttatgaaa gattataaaa tcaattttga tttaggtaaa atagaatatt
    52381 ttgataataa ttgtttgata caagtataca aatttatttc tttttatgat atttgtgaaa
    52441 tggtgtttgc ctttcattta cctcctgatg agttgataac aaatgttatc tttaaagaaa
    52501 aaattaactc tatgttaaaa tgttacattg acaggctatt atatgttttt ataaatccta
    52561 cacattttac tgaaaaagtt aatctacaat tttatggcag ttttttctca tatgaattta
    52621 tttgtcgtga agttggaaac attcttaaaa ataaaggtgt gaaatgcaat ttaaattttt
    52681 ttgaagggga agaatatttg taaaattaat tttgtgaatt tgtggatctt aaataattta
    52741 ctaaattctc ttgttaaaaa aaattaaata ttgtatagta atattagaat gtttgtaata
    52801 gaggactgaa atgaaaaata taaatgggaa aaaggtaact atttatgata ttgcacgttt
    52861 atcaggtttt tctccaaaaa cagtttcacg agttattaat ggtggagtaa atgttaaaga
    52921 agaaacttat caagcgattc aaaaagttat agaggaattg tcgtatattc caaatgcgta
    52981 tgcaaaaaat ttaactaaaa aagaagctat aaatatattg atttctgtaa aaaaaatcga
    53041 ctcttttcct ttaatttggt ttcatacgct gttagataaa gtgttacgga cgtgtaaaga
    53101 atttggtgtt aatgctatag ttgagtactt tggagaggag gatacaatta gtaattcaat
    53161 tatatcaagc acaggtagcc tagtagatgg tgttattgtt ttttatgaaa gtgtagatga
    53221 tatcagaatt cagtatttaa agaagaacca tatgcctttt cttgttttcg gtgaatctca
    53281 aacgtctgga gtagtctatg tatctaataa taattttcaa gctacttatg atatgatgaa
    53341 agctgtaact gaagaaaaat ttaaaaatat gtggttgctt atggggggag aatcccatgt
    53401 taataaggat cgtgaaagag gtgtgcgttc ttttctgaat gataaaaatt actttatgga
    53461 tttaaaagtc atttatggct tatctacaat tgattcagtt tattcttatg caatgcagca
    53521 cttgactacc caaaattatc cggacattat ttttgtttca ggagatgaaa aggttcaggg
    53581 cttgattcgt gcgtgctatg agaaaggaat tcttattcca gatgatattt ctatcattgg
    53641 atttgataat attcctattt ctcagtatta tactccaggt ttgtctacaa ttgctcccaa
    53701 ttatgttaaa ttggctaaag aaatgataga aggtgtttta gcaattatta aaggggaaag
    53761 tgtcacatct gttgaagttt ctcctaaatt tgttaggaga cagagtttct agtattgttt
    53821 aattcttgaa aatcctagtg gaaaagatgg agtgttctaa aataagttct gtttagcctc
    53881 cattcagttt ctattgagtt ggatccaatt tctaagaagt gggacatgtt catgttcgat
    53941 ctaaggaaga catggtcaca ttcgaatgat attttcaaaa aaatcgtaaa aaagtcttga
    54001 caaagaaaaa acaaagtatt aaaataagtt caacaaatca gaaaagtaac tatttttgat
    54061 cttcagggag cctgtggtgg ttgtgaatag gtggttggaa gtagtgaaag tgggctgatt
    54121 ttgaaaatga atttgaaaca atgaaaattc ggtgcgcaca ccttacagtg cagcttgttg
    54181 ttaaacaagg cagagatgta aagggatagt ccctttataa ttgaggtggc accgcgttac
    54241 caacgccctc acacggaagt atattctgtg tgtgggcttt tttctatccg tcgtttggtt
    54301 tatcttttat tagggcctta agtcgctttg atgaacttga gttctatcta cagctccttg
    54361 cctactacta aaagcaaaca aaaaggctgg actaatacga aaaaagctag taatactcaa
    54421 tgaaaatcaa agagcaaact aggaaactag ccgcaggctg ctcaaaacac tgttttgagg
    54481 ttgtggatag aactgacgaa gtcagtaacc atacctacga taaggcgacg ttgacgcggt
    54541 ttgaagagat tttcgaagag tataaaggtg taccgagatg aacatctctt catcaatgtc
    54601 tctggtcgtg gaggcaagga cctagctgcg attgcagatt actagaagtt aaaaaataat
    54661 agatggaaaa attacagttc tttctctcac agagagcttg tggttgctga aaacaagcag
    54721 agaaagctgt aaagttgggt tcatctgaaa cgagagaaga aaacatcatt ctcaagggag
    54781 tgccctttat cgcacagcct gttacaggag atatctgaga caggaatgag agataggaga
    54841 aatcctataa ttgaggtggc accgcgaatt tcgtcctcac gcaagttatt ttgcgtgggg
    54901 atttttcata cagtcaccac ggactagaag tagaactgaa agggaaatta caatggaacg
    54961 aatcattcat ggagatgtct tatcaccaat cttggcttat atgcgcttaa aggggcaaca
    55021 taaggttatt ttagagagta ttccgagaga caaggaaaca gctcgttttt ctatcctagc
    55081 ctataatcca gtttttgaga ttaagtttga aaatggggtt ctctatcaaa atggtcaagt
    55141 gattgatcgg gatcccttgg atttccttta tgaagtgatt cataagagtc agcaccactc
    55201 agagctacct tttggtggtg gagccattgg ttttgtgggt tacgatatga tttcgcttta
    55261 tgaagaaatt ggtcaaatcc ctgaggatac aattgggacg ccagacatgc atttctttgt
    55321 ctatgagagc tatatggtct ttgaccacaa gaaggagaag atacatgtca tagaggatgc
    55381 actttatagt gagcgcagtc aagaggcctt ggaaaaatcc ttgaaccaag tgcttgagga
    55441 attacgcatt cctgctccaa atgaatttga agatttggat ctatctccgt tagacttcaa
    55501 accgcatatc gctccacata agtttgaggg aatggtagaa acagctcgtg acttgattcg
    55561 taacggagat atgttccaat gtgtactcag ccagcgtttc tcagcagaag ttactggaaa
    55621 tccatttgac ttctacagaa atctccgcgt gactaatcct tctaattacc tttatttcta
    55681 tgattttggg gattatcaaa tcatcggagc tagtccagaa agtttagttt ctgtcaaaaa
    55741 tggcatcgtg acaaccaatc cgattgcagg gacgcgacca agaggggcta cggatgaaga
    55801 agacaagacc ttggcgactg acctcctctc tgatgagaag gaaacagcag aacatcgaat
    55861 gttggtagac ttggggcgta acgatattgg ccgcatctct gaaacggcca gtgtccaagt
    55921 tactaagtat atgaaggtgg agctcttccg ctacgtcatg catttgacca gcgtggtcaa
    55981 ggggcgtttg cttccagaac tcacggccat ggatgccttg aaagctacac ttcctgctgg
    56041 aaccgtttct ggagcaccaa agattcgggc gatgagacgc atctatgaac tggaaacgga
    56101 aaaacggggc gtatatgcag gagcaatcgg ctacttgtct gcgacgggtg atatggattt
    56161 ggccattgcc atccgaacca tgattctcaa aaatcaaaga gcctatgtgc aggctggggc
    56221 agggattgtc tacgactcta tcgcccaaaa cgaataccaa gaaaccatta acaaaacaaa
    56281 atctatgact agaattggag aactaagacc atgattttat tgattgacaa ctatgattct
    56341 tttacctata acttggccca atacattggg aattttgcag aagttcaggt tctgagaaat
    56401 gatgattcca agctgtatga agaagctgaa aaagcagatg gtctggtctt ttctcctggt
    56461 cctggttggc cagttgatgc tggaaagatg gaagacatga ttcgtgattt tgcaggcaag
    56521 aagccgattc tagggatttg tttgggccac caagccattg cagaagtctt tggtggtaag
    56581 ctaggtttgg ctccaaaagt catgcatggg aaacagagca atatcaactt tgaagcgcca
    56641 tctgttttgt atcaaggtat tgaggaagga cgtgcggtca tgcgttatca cagtattttg
    56701 attgaggaaa tgccagaaga ctttgaagtg acagctcgtt cgactgatga ccaagccatc
    56761 atggggattc aacataaaaa cctaccgatt tatggcttcc agtaccatcc agagagcatt
    56821 ggaacgccag acggcttgtc ttctattcgg aattttatcg agaaggttgt aaagtgagga
    56881 aactaggatg aaagagatta ttgaaaaact agcaaaattt gaaaatttat caggtgtgga
    56941 aatgacggat gtcattgagc gtatcgtaac tgggcgtgta actgaagcgc agattgcttc
    57001 tctcctctta gctcttaaga tgaaggggga aacacctgaa gaacgcacag ccattgccca
    57061 agtcatgaga ggacatgccc agcatattcc aactgagatt catgatgcca tggacaactg
    57121 tggtacaggt ggggacaagt ctttcagttt taatatttcc acaactgcag cctttgtctt
    57181 ggctggtggc ggtattcaca tggccaagca tggtaaccgc tcgatttctt ctaaatctgg
    57241 ttccgcagat gtcctcgaag ccttgggaat caatcttgac ctcaaaccag ctgaactagg
    57301 taaggtcttt gataaaactg gaatcgtctt tctcttcgct aaaaatatgc acccagctat
    57361 gaaatacatc atgccagctc gtttggaatt gggaattcca acgattatga acttgactgg
    57421 tccactgatt catccaatgg ctttagaaac acagcttctt ggaattagtc gtccagaact
    57481 cctagaaagt acagctcagg ttttgaaaaa tatgggtcgc aaacgtgcca tcgtggttgc
    57541 tggaccagaa gggttggatg aagctggctt gaacggaaca accaagattg cacttcttga
    57601 aaatggcgaa atcagcttgt caagctttac tccagaggat ttgggaatgg aaggctatgc
    57661 tatggaagat attcgtggtg ggaatgctca ggaaaatgca gaaattttgc ttagcgttct
    57721 gaaaaacgaa gcaagtccat tcttggaaac gacagtcttg aatgctggtc ttggtttcta
    57781 tgctaatggt aagattgata gcatcaagga aggagttgcc ttggcccgtc aagtgattgc
    57841 tagaggcaag gcccttgaaa aactcagact gttacaggag taccaaaaat gagtcaggaa
    57901 tttttagcac gaatcttaga gcaaaaggcg cgtgaggtga agcagatgaa gctggagcaa
    57961 atccagcctc tgcgccagac ctatcgcttg gcagaatttt tgaagaatca tcaggaccgc
    58021 ttgcaggtaa tcgctgaggt caagaaagct agccctagtt tgggagatat caatctcgat
    58081 gtggatattg tgcaacaggc ccagacttat gaagaaaacg gagcagtgat gatttcggtg
    58141 ttgacagatg aggttttctt taaagggcat ttggattatc tacgggaaat ttccagtcag
    58201 gtagagattc cgacgctcaa caaagacttt atcatagatg aaaagcaaat catccgcgct
    58261 cgcaatgcag gtgcgacagt tatcttgctc attgtggcag ccttgtccga agaacgcctc
    58321 aaggaactgt atgactacgc gacagagctt ggtctggaag tcttagtgga gactcacaat
    58381 ctagctgaac tagaggtagc ccacagactt ggtgctgaga ttatcggggt caacaaccgc
    58441 aacttgacta cctttgaggt cgacttgcag accagtgtag atttagcccc ttactttgag
    58501 gaaggtcgct attacatttc tgaatctgcc attttcacag ggcaggatgc ggaacgacta
    58561 gccccatact ttaacggaat tttggtaggg acagctctta tgcaggcaga aaatgtggcc
    58621 cagagaatca aggagttgca gattgacaaa ggttaaaatt tgtggactat cgaccaaaga
    58681 agcggtggaa acagccgttt cagcaggagc cgactatatc ggttttgtct ttgcacctag
    58741 taaaagacag gtgactttag aagaggcagc tgagttggca aagcttattc ctgcagatgt
    58801 gaaaaaggtt ggagtatttg tttcaccaag tcgggtagaa ctgctggaag cgattgacaa
    58861 agttggcttg gacttggttc aagttcacgg tcaggtggca gatgatttat ttgagaattt
    58921 gccttgtgct agcattcagg ctgtgcaggt agatggagag ggtcatgtgc ccaattctca
    58981 ggcaaactat ctactctttg atgcccctgt ggctgggagt ggccagccct ttgattgggg
    59041 tcaactggat acgactggat tacctcagtc tttctttatc gcaggtgggc ttaatgaaga
    59101 caatgtagta aaagcaattc aacactttac tccctatgca gtagatgtat caagcggagt
    59161 ggagacagat ggacaaaaag atcatgaaaa gattagaaga tttatagaga gggtaaagaa
    59221 tggcatatca agaaccaaat aaagatggat tttacggaaa attcggcgga cgttttgtcc
    59281 cagaaacatt gatgacagca gttttggagt tggagaaggc ctaccgtgaa agtcaggcag
    59341 acccaagttt ccaagaggaa ttaaaccaac ttttgcgcca gtatgtggga cgtgaaactc
    59401 ccctttacta cgcaaaaaat ttgacccagc atatcggcgg agccaagatt tatctcaaac
    59461 gggaagacct taaccataca ggagcccaca agattaacaa tgccttagga caagtttggc
    59521 ttgccaaacg catgggtaaa aagaaaatta tcgcagaaac gggtgctggt cagcacggtg
    59581 tggcaactgc aactgctgca gccctcttta atatggaatg taccatctac atgggtgagg
    59641 aagatgtcaa acgccaagct ctcaatgtct tccgtatgga gcttttggga gctaaggtcg
    59701 aggccgtgac agatggttcg cgcgtgctca aggatgcggt caatgcagcc cttcgttcat
    59761 gggtagctaa tatcgacgat acccactata tccttggttc tgccttgggg cctcatccat
    59821 ttccagaaat cgttcgtgac ttccaaagtg tcatcggtcg cgaagctaaa caacagtatc
    59881 gcgatatgac aggtcaaaat ctgcccgatg ctctagtagc ctgtgttggt ggtggatcta
    59941 atgctattgg gctcttccat ccctttgtag aagatgagtc agtagctatg tatggaactg
    60001 aagcagcagg gcttggtgtg gacacggagc accacgctgc taccttgacc aagggtcgtc
    60061 cgggtgttct tcacggttct ctcatggatg tgctccaaga tgcccatggt caaattcttg
    60121 aagccttctc tatctcagca ggtttggact atcctggtat cggtccagag cattctcact
    60181 accacgatat taaacgtgcc agctatgtcc ctgtgacaga cgaagaagcc ttggaaggat
    60241 tccaactctt gtctcgtgtg gaagggatta tcccagcttt ggaatctagc catgctatcg
    60301 cttttgcggt gaaattggcc aaagaacttg gaccagaaaa gtctatgatt gtctgcctat
    60361 caggtcgtgg ggacaaggat gtggttcaag tcaaagaccg cttggaagca gatgcagcaa
    60421 agaagggaga agctcatgcc taagacacta acagaaaaat tgaatgctat taaagcagct
    60481 ggaaaaggaa tttttgttcc ctatatcatg gctggtgacc acgagaaagg tttggacggt
    60541 cttgctgaaa caattcactt tttagaaaat ttgggtgtct ctgcaattga agtgggtatt
    60601 cccttttcag accctgttgc agatggacct gttatcgaag aagctggctt gcgcagttta
    60661 gcccacggga cctctaccca ggctttagtt gaaaccttga aaaccattga aacagagatt
    60721 ccactggtca tcatgaccta cttcaacccc ctctttcagt acggtgtgga gaactttgtc
    60781 aaagatctgg ctgatacagc tgtgaagggc ttgattatcc cagacttgcc tcatgaacat
    60841 gccaactttg tagaaccatt tttggcagat acagatatcg ccttgattcc tttagtaagc
    60901 ttgaccacag gaattgagcg ccagaaagag ttgattgaag gggcggaggg attcatctat
    60961 gccgttgcca tcaatggagt gacagggaaa tcaggcaatt accgtgcaga tttggacaag
    61021 cacttggcgc aacttcatca agtggccgac atcccagtct tgacaggttt tggtgtatct
    61081 agtcaagccg atctggaacg cttcaatgcg gtgtcagatg gtgttatcgt cggttcgaaa
    61141 atagtaaaag ctctccacca aggagagccg attcaggact ttatcaaaca agcagtagct
    61201 taccaaaaat aatcaaacaa gtagcaagta agaggaaagg cacgaaagga agtctttcct
    61261 ttttcttttg caggcgaaag gctagtttta tagtgaattg aatctggaat attacactac
    61321 ggttcctaaa atattttaag aaagtgattt gaatttccca ataaatttgt tcagttttta
    61381 attgatttta ctatagaaga tataattttc taaattactc tgacacagaa ctttaagaaa
    61441 aagctaatta ttgtagtaat ttctttaaga aaatgatata attcattaaa aagtatttgg
    61501 aggaggaaga attatgttga atcaggatct ctttgattcg cttgaggcac aaaaaattgt
    61561 agatactttg atgaaagggc aaaaagatta tgtagatgaa cgtctagaaa aaagagagac
    61621 aatgatagtg tcgaatggtt atgcatggac gtgacctaat catattgata ctgcatttgc
    61681 atcagcagat ttgtttgagt ataaattaca attagcagga cagacttggg gatatttaga
    61741 atttgaaaca aatacagaaa aatatgggaa agtattgtta atgataaagg gtaagaagcg
    61801 acttacgaac caatttcctt tggtacaaaa aaataagagt ggctacttat ttgaatacgc
    61861 tcagatgaat acactttatc ttaatcaaca ttcttcctac aaaaatgatg aagatagtca
    61921 ttcctttcca attcagatgg agttagtttc tgatgaaatg attcaagaaa ttgaacaagc
    61981 tactaaaaat tcgaatatcg aaaaatttat gattttaact tatgaggcgg actcagaaaa
    62041 caatattata tctgtagatg ttgttatgcc tgatgcacga actggtcagt tacacttgat
    62101 tcaagatttg tctgagtata ttcaatcaag ttcgtaccat ttcgaagaag ctaaatatca
    62161 agatattcct aatttttcag aattatcgga aacagaagat tttgaaatta ttccaagaat
    62221 agaaaaacaa gaaggtcaaa agtagtaagg agtatgtaaa tgtttaatgg tcgggtattg
    62281 aaagaattac ggctgttaaa tggtttaagt agagcagaat tagctcagag aattaattta
    62341 acggaacaag ccatttggca gtttgagtcc aacgaaacga aacctaaatt atcaaccaaa
    62401 atgcatttgg ccaaccaatt tcatgttgat ttaacttatt ttgaacagga agaagagagc
    62461 attcgatttg attcttctgt aattgccttt agaaatgcag acctagcaac acggaaaaca
    62521 atagatattc aaactatgta tttacataag gtagatagtt tgattgatta ttttgaaagt
    62581 tttgtaatta tacctaatat tataattcat gacctaagta atgtagtgag tgaatcttat
    62641 cataagggag aatccattga ggaattggct ctttatgcca gggaaaaatt aggtatttca
    62701 aaagataatc atgatttgct ttataaatta gaacgttcag gcatctatat cgtggaacga
    62761 ttaattaatg gccaagctga tgcttatagc gcatggtcaa aattgggaag accttatatt
    62821 gtgttgggaa cgaataaatc atctgtacgt cgaaattttg acttagctca tgagctagga
    62881 catattcttt tacataaata taaagatatg aatgaagatg gcgatcgttt ggagcaagaa
    62941 gcaaattatt ttgcatcatg ttttttattg ccaaaagaag agtttttagt caaatttgaa
    63001 gagagggttg gcaagcgtgt cagcaatcct gatagttata ttttattgaa gtcggatttg
    63061 aatgtttcga tacaggcttt agagtatcga gcttttaagt taggattatt gactccaaag
    63121 caacattctt acttttatcg tcaaattgcg caaaaaggtt acaaaatgat tgaacctttg
    63181 gatgatcaaa tttttgttaa aaaaccaagc aaagtaaaga gtattctgga cgttgttttg
    63241 agtaatcatc tagtcagtct agcgactata atgtctaaac aaagtattcg tttacagttt
    63301 ataagcgaaa tattttcagt cgaaatgaaa ttttttgatc agtatcaaga agatagaaga
    63361 acagatcgat ttgataacat catccctttg tacaaaagaa ataatttata atttaactgt
    63421 gaaataattt catgaaggac atatggcaga aattggattt tatcagacaa gcagtagctt
    63481 accaaaaata atcacacaag cagcaagtaa gaggaaaggc acgaaaggaa gtctttcctt
    63541 tttcttttgc aggagaaagg ccaggatacc cgtcgcagaa gcgaactgaa tcaagatcag
    63601 taactccgtt acgctaaaga cgagagcgca agaagctaaa aaaaggaaat cccctgcacc
    63661 catgcggata tcgataaaat gagccaaaat tccaagtatg aggaaggaga ccatgaccag
    63721 attccagcca gaggaagcta ttaggattag gtggaaagtc atccagacca gtaagggata
    63781 ttcctgatgg cgaaagtcgt agatgcccaa ggtcaaacca gcagtgatta ggatgacttg
    63841 actcaaggaa agtaattccc aagagtaaag cagaaagagg agtcctaagc ctagttcaaa
    63901 gagggcatac cagacaggat agcggacctt gcagtagcga cagcaaaagc gattgaagac
    63961 ctgtgagaga atcggaatca aatctaaggg acgcaagcga gtctgacagg aatcgcagtg
    64021 actggctgaa ctgataatgg attgctctgg aaaacggtca atgaccaaac caagaaagga
    64081 agcgagaatg ctcccgacga gaaaaaaata aaaatcaatc atacttatct attcgtaaaa
    64141 aatgggagaa atagtataat ggagaaacat agataagatg gtgaggaaaa gatgacaatt
    64201 cgttttgaag aaaaggtgag tatagaaaac gctcaatttg tatgccagtg gtccaactcc
    64261 ctaggcaaat cctttcaaga acaatggatg ggaccaagga ttccttttct actgaccctt
    64321 caagccttgg aaggagtctt ttcaatcttt gatgaacaag agtttgtggc acttatccag
    64381 aaaatcaggc tagaagacag caatcttcat atcgggagat tttttatcaa cccccagaaa
    64441 caggagcaag acttaggtag ccaggcttta aggaaatttg ttagtttagc atttgaaaat
    64501 agagatatag acagtatttc tctaaatgtc ttcgaggcaa atcaaagagc tcagaatctt
    64561 taccaaaaag aaggatttga aatcgtttaa atggttgaag cacctgtacg aaaatatagt
    64621 agattgaaac tagaatagta cacctctact tctaaaacat tgttagaaat cgatttgact
    64681 gtcccgatcg attcgtcctg ttattatttc attttactat acattatgaa aaatgggaga
    64741 taggagtata attccaaata taaagaaagc gaacaaaaca gaattctgat tttcagaaaa
    64801 caggtttatt cgctttttat gatatactcc ctctttttag cctatttatt aaatgtaata
    64861 gaaaattaag aatgtagaat tgtatgatac aaacaaagtg caatagctga gtgtgcggct
    64921 gtaaaatcat tgtaaggttc aataatgact tcaggtaggc gattatagag cagtttgggt
    64981 ttgtagttca tttcactata tagtttttgg atgatgtgtt gatttgttag taaaggacta
    65041 tgtaggtaga ttttttgaga gtcaatcatc atactgatat ttaaaatggt ttgactaaga
    65101 tagagtaagg cttgatggat aagtgtgatt attccagtgt cacctaattg ataagcggtt
    65161 aaaataactt ggatatcaat atcatcggca tttttgacta aactaggaag tagagaatag
    65221 ggagattgat gatatagaat ttttgatttt ttgattaacc aagattctcc agcaaaagtt
    65281 tgtaagcatc ccttgcgccc gcagctacat tcttctccct cagatgaaac aactgtatgc
    65341 cctatttctc caatcattag atttcctttc ccataaatgt ttccgtcgta gatataagaa
    65401 cagtgcatac ctctagcaaa atgaaaataa gcaaaattag ggtcattctg ttggcgacta
    65461 aataggcgtt tacctatagc catacagtta acattgttag agaaaaacaa aggcttatca
    65521 aagtgggatt gaatcatttc taagtcgata tgttgccata aaggattgtt tgttgttatt
    65581 ttataatcat ctaaatagcg accaggtagc gcgattccaa ttgcttctat ttcataatca
    65641 gagcagttat ttaagaattg ctttagtgtc tggttaatga gttgatttcc cttttcttgg
    65701 attaactgtt tagtaacaat ttctttctct tcctttaaga tatttcctag attatctcct
    65761 aaagcaaaag tgaaatgttt ttcagatagt tcacaaccaa tgtagtaaaa tcgtttagct
    65821 tgaatgtcta gcaggatttt ttttctccct acgcttgtat cgtgttcgtc ttcaccaagt
    65881 tctaagagaa tattttcttt aatcagatca tttgtgatac tgctagttgt tgcaggtgtt
    65941 attcctgttt tagtagcgat ttcaattcgt gaaatgggac ctaatgtgta aactgtttcc
    66001 agtattttag ctcttagttg taattgtttc ttagataagg tcatattagt tttcttccta
    66061 gttgtcagat tcttaaaatc ctatgatttt ttgctatgga taaatgtaga tgttaatctt
    66121 tttaagcttt tcattactta ctggtctctt tttattatat attgataata aaaaataatc
    66181 aaatgtttac acaaatcctt gacaaggctt tcttatagtt gtaaaatata aataacaaaa
    66241 gataatcaaa taacaacttg acgtcaaaaa ataaagataa ttgataactt ttgataataa
    66301 aatatattta agaggaaaag gttatggaaa aattattaca agaaaagtta cttccagtag
    66361 cggctagatt gggtaataat aaagcgttgg tttctattcg agacggtatt actctaacta
    66421 tacctttatt gttgataggt tcattactaa tggttattgc aagttttcct attccaggat
    66481 gggaaaaata tctgggtgat attggtgttg ctgactattt atggaagggt gttgatagta
    66541 gttttggatt acttggtctt gtagctagtt ttggtatcgc ttactttatg gctagacaat
    66601 acaaagttga tggtattcct gctggtattg tgtcattatc atcttttatt acagttacgc
    66661 ctttcattag aggagaggct ggagcaggaa tgcctaccgc tttcatggca tcaaaaggtt
    66721 tatttgttgc tatgatttta ggattgatta atggctatat ctaccaatgg tttataaatc
    66781 ataatataca gataaaaatg ccggatggtg ttccaccagc agtatctaaa agctttagtg
    66841 ccattattcc tggtgcagtg actattgttg gttggttgat tgtttatgca actttagata
    66901 aattaagttt acctaatctt catgaaattg ctcaagtagc tttgggaggt ccacttggac
    66961 ttttaggaaa taatgttatt ggtcttctta tcttaatttt ccttaatagt agtttttggt
    67021 ttgtaggatt acacggagga aatgttgtta atgcagttat gaaaccgtta tggttagcta
    67081 atctagatgc aaataaagta gcttatcaaa ctggagaaac tcttccaaat atttttacta
    67141 gcgtatttat ggataatttt gtattcatag gtggcggtgg tgcaaccata ggtttagttc
    67201 ttgcgttagg atatttagca cataagaaga aagctagtaa acaactaaaa actttggcac
    67261 ctataactgt tatacctgga ttatttaata taaatgaacc agcaatgttt ggcgttccta
    67321 ttgttttgaa cattttgtta ctagttccat ttatcttagc tccaatgttt aacttactag
    67381 tagcgtgggg agcaatggca tcaggtttgg ttccgctaac ttatacagat ccaggttgga
    67441 ctatgccacc tgttataagt ggtttacttg ctacaggaag tatttctggt tcattattac
    67501 aaattgtatt gattgttttg gatgtcttac tttatttacc atttgtaata gctattgaaa
    67561 aacggtttaa gttattggag gattgagaat gaaacgttta attagtgcaa atccatctga
    67621 gatattacag atgaatgctg aagaattaaa acaaagtatt ttagcaagtg aaggtagagt
    67681 tgttctatct gaaaatgtag ttactcgtga gacatttgtt ggggatataa ctaattctga
    67741 aattgctaga gcttttggag ctgatatgat tttattgaat tgtgttgatg tttttgagcc
    67801 taaaatttat gctttggata gttcaggtga tgatgttatt catcgcttac accagcttgt
    67861 tgcttgtcca attggtgtaa atttggaacc gattgaccca tctgcaaaga tgctagagga
    67921 aacacaggaa attgttgcag gtcgtgttgc tagtgttgaa acattgaatc gaatagagga
    67981 gttaggtttt gactttgtct gtttgactgg aaatcctgga acaggagtta gcaatcgaga
    68041 aatcattaag gctgttcaaa ctgctaagga aaacttttct ggtttgatta ttgcaggtaa
    68101 gatgcacggg gcaggagtga atgagcctgt ggcagagctt tctgtcgcag agcaattgtt
    68161 ggaagcaggt gcggatgtga tacttgttcc agcagttgga accgttccag cctttcatga
    68221 ccaagagttg cgtgaagtcg ttgatctcgt tcatagtaag ggtggtcttg tattgagtgc
    68281 tattggtact agccaagaaa catctgatac agatactatc aaggaaattg cacttagaaa
    68341 taaaatttgt ggagttgata ttcaacatat aggtgacgca ggatatgggg gactggcaac
    68401 agtcgataat atttatgcat tgagcaaggc aattagagga gtgagacata cagtatctcg
    68461 cttggctagg tcagtaaata ggtgataaag gagtaagcta tggctaaagt aacaattatg
    68521 ttagcatgtg cagcaggtat gagtacaagt ctgctagtga caaagatgca aaaggcagca
    68581 gaagataagg ggttggatgc agaaattttt gcagttccag ctcctgaagc agaagaaatt
    68641 gtagcaacaa aggaagtaaa tgtgttgctt ttaggccctc aagttcgcta tttactaggg
    68701 gactttcaag aaaaactaaa agatagacag attcctgtgg cggttattcc gatgacagat
    68761 tatggaatga tgaatggttc taaagtctta gatttagctg aaagtttatt agactaagat
    68821 tgaggagaat gctatgaatg aaagtaattt agaatctgca atggggctaa ttatgtatgg
    68881 tggggaagcc aaaagtaatg ctatggaggc tattcaggca gcaaaaaaag gggatttctc
    68941 taaagccaat cgaagattag ctgatgcgaa tgctgcctta ttacaggcgc ataaggctca
    69001 aacagaaatg ttgacaagag aggcacaggg ggaagaaaca tcaattagcc tcttgatggt
    69061 acacgcgcaa gatcatttaa tgacgagtct gacttttgtt gacttggcga aagaagtggt
    69121 agaagtatac gaacgatttg aaaaaaatta ggagtttagt atgaatacaa tgttggataa
    69181 aatgcaagaa aaactttctc caattgcaat gaaagttggg aatcagaagt ttttagttgc
    69241 tttacgtgat tcatttgtgg ggactatgcc tgttattatg acaggttcca ttgctttgtt
    69301 attaaatgct tttctagtgg atttaccaca acaatttcac ctagaaagta ttacgaaaac
    69361 ctttcagtgg cttgttgaca tcaataattt ggtattcaag ggaagtatac caattgtttc
    69421 cttgttgttc atctattgct tgggagtgaa tatcgctaag atttacaagg ttgatacagt
    69481 ttctgctggt ttggtatcac ttgcttcgtt tgtcatttcg attggaagta cagttacaaa
    69541 gagtttccct ttggcaaatg taggagatgt gaaattagat cagatcttac aaggaattga
    69601 taatctagca tttgatggta aaaatctgat ggtaactatt gggaatgtga ttccaggaaa
    69661 ccatattaat gccagaggct actttacagc catgatgatt ggatttttag cctctattat
    69721 tttctgtaaa gtaatgaaaa agaactgggt aatcaagtta ccagattcag ttccccctgc
    69781 gattgctaag ccgttcactt ctatcattcc aggatttatg gcgatgtata tagtagccat
    69841 cttgacttat gttttccatt tacttagcaa tgacttattg attgattggg tttacaaggt
    69901 gttacaaacg ccattactag gtttgtctca aagtttcttt gcagttattc tgatgatctt
    69961 tttgaataag ttattctggt tttttggtct ccatggagga aatgtattgg ctcccatcat
    70021 ggaagggcta tttggagttg ctatgttagc gaatctggat gctttccaaa aaggtgaacc
    70081 gattccttat atatggacaa gtgggtcttt tggggcgttt gtctggtttg gaggattagg
    70141 attagtactt gctattttaa ttttttcaag aaacagtcat tatcgaaaag ttgctaaact
    70201 tggtctagca ccagttctgt ttaatattgg tgagccggta aattatggtt taccagttgt
    70261 cttgaatcct ttgctattta taccatttgt acttagcccg gttttcatgg caacggtagc
    70321 ttactgggca acaagctggg gtcttgtttc acctgttaca caaaatgtta cttgggtaat
    70381 gcctccaatt ttatatggtt tcttctctac agcatttgat tggcgtgcca tcatcttatc
    70441 agttgtttgt ttgattatta gtgtcttgac ttatttccca tttgtgaaaa tggcagataa
    70501 aactgaattg agttaatggt cttacttgcg ttttttgtga taaaatagaa atagattacg
    70561 tgtaagatga gttaggtgta ggatttctta tctccactaa aattgttatt agacaaaggt
    70621 ttataattta ttgaagttgg aatagtacac tgtaacttct aaaacagtac tagaaaatga
    70681 tttgaatttc ctaatcgatt tagtcatatt ttagtccatt gttctataaa acataacaag
    70741 aatgttattg ctttatatag agagattgat atagaaaggg gtgcagtaaa ggtagatatc
    70801 ttcttgtgcc tcttttgcca tattagaaag gagattttat ggttcagaca aaacaaccaa
    70861 atattatttt gattgttgta gatcaaatga gagcagacgc cttatccttg aatagtaagg
    70921 ataagctagt tagcactcca acattagata tgatggctag tgtaggttat aattttgaaa
    70981 atgcctattc tccagttcct tcttgcgttc ctgctagggc agctttattg actggcttag
    71041 accaagataa aagtggacga gttggttatc aagatgaggt gccttggaat tttactaata
    71101 ctcttccaaa agtctttaaa gatatgggtt atcaaacaga gtgtattggt aagatgcatg
    71161 tttttccttc cagacagaga cttgggtttg atcatgtctt acttcacgat gggtatctac
    71221 atgttgatag aaagtatgat aaggcttatg gaagtcaatt tgattatgct agtgactatt
    71281 tagccttttt aaagggtaaa gttggttatg atgttgattt gattgatgac ggaatggatt
    71341 gtaattcttg ggaagctaga ccttgggata aagatgagaa gttacatcca acaaacgggg
    71401 ttgtgagtga gtccatttca tttttacaaa gaagagatcc aacggttcct ttttttctca
    71461 aaatgtcttt tgaaaaacca catgctccat taaatccccc aaaatactat tttgatatat
    71521 atatggaacg cttgcctcaa tttctcgatt tgcatatcgg gaattgggaa gtattagaaa
    71581 agcaaattcc gtctatttat gctttaagag ggaaattaaa ggaagatgat caaagaagaa
    71641 tggttgctgc ttattttgga ttgattactc atatagacca tcaaattagt cgctttttga
    71701 ctgccctaaa agaatttcgt catgataaag atacgattat ctggtttgtt tcggatcatg
    71761 gagatcagct tggtgaacat tatctgttta gaaagggata tccttatcag ggaagtatcc
    71821 acattccttc gtttatttac gatccggctg gattgatagc tggtaatagg ggaacaatca
    71881 agcaattagt gaaaattcaa gatatttttc cttcactcgt ggatttggca ggaggaacta
    71941 ctacagatga actagatgga cggagtgtta agaatttatt atttggacag tacgaggggt
    72001 ggcgaacaga atttcatggt gaacatgctt taggaaaaga ttctagtcaa tatattttga
    72061 cagaccagtg gaaatttatc tggtttcctg ttttaaatca ttatcagtta tttgatatga
    72121 aaaaagatcc gcatgaaatg aatgatttgt atccaagtga aaaatatcaa cctattgttc
    72181 gtcagatgaa gaaaaaatta gtagactttc ttagatatag agaagaaggt tttgttgtgg
    72241 atgaagagtt agttcctgtt gaactttcta aaataacgcc aactctaacg aaaacggggg
    72301 atagtcaatc atgaaaatga aaaaaatcaa gggtgggaca tttatgatgg gaacaaattc
    72361 tgaagagggc tttttagatg attttgaagg tcctcaagtc gctgttagtg taaaggattt
    72421 ttccattgcg gatactccag ttacgaatca agaatttgct caatttgtga aagaaacagg
    72481 ctataaaaca ctagctgagc gacaagagtg gtcttttgtt tttatattgt tcgttccgga
    72541 ggcagaaagg gagggatatc ctcatcctgc tggggctccg tggtggttac aagtttcgaa
    72601 cgcttgctgg aaacatccct acggtgagaa cagtaatcta gtgggtttag aagaccatcc
    72661 agtagttcat gttgctttag aagatgcatt ggctttctgt aattggagcg gaatgtcttt
    72721 accgactgaa gcacagtggg aatatgctgc tagaggagga agacaatctg aatatccttg
    72781 gggagatact cttttagaag gtggatatta tcatgccaat acttggcaag gaagatttcc
    72841 ttatgaaaat actgccttag atggatttat aggaacggct cctgtctatg aatttttacc
    72901 taatgatttt ggtctatatc aaatgattgg gaatgtttgg gaatggtgtc gcaatccgag
    72961 atatacctta ttagctagtt ttaatgaaga tgactatgag ttaccaaaat atgggataca
    73021 agatgaggag tatgctatca gaggaggttc ctttttatgt cattgttctt attgtaatcg
    73081 ataccgagta gctgctagaa atggatgtat ttctacttcc actagtagcc atctaggttt
    73141 tagatgtttg aaagaatagg atggacttaa aaagtggttg agattattta ttttctaatc
    73201 attataatag ctagtggatt gggttctata tcaggcatgg gtggtggaat tataattaag
    73261 ccacttatgg atagttttgg gtaccattcg gtcagtgata ttgcttttta ttctagtttc
    73321 tctgttttta taatggcgat tatttcaaca atcaaaagat tttcacaaag taaagagata
    73381 aaatggagat tgatttttac ggtatccttt tcttctgttt taggaggatt tctaggtcat
    73441 cttatttttc aagttttgtt atctcaatta tcagtacggc tagtatccat tgttcaaatg
    73501 attttactat ttgtgatgct acttgtatca tttgttttaa cagattttaa aaaaacttat
    73561 caatttgata agatagaatt ctatatgatt tgtggtcttc tattgggctt aatttctagt
    73621 tttttaggga ttggtggtgg tccgctcaat gtatctttat tgatggtgtt tttttctatt
    73681 tcgataaaag aagcaacgat gtattctctt gcaattattt tcttttccca gctgtctcat
    73741 ttggctacaa ttgttgtggt tacaggttta aatcagtatc atttagcacc tgttccagtt
    73801 atttttcttg cttctatatg cggaggggta ttggggacgg ttgtatcaaa agtcttacct
    73861 gaaaattggg taagatactg ttttaaaggg atgttattct ttgtcatggg aatgacttta
    73921 tataatttat ttcatatttt gtaaggtcag tgtgaaaata tttaaataga cttcctgtaa
    73981 aaactggatt taacgccctc catttgtcaa attaagctag acagtcttac caactcagaa
    74041 aatacctgat gaggtatttg gtatccaaag gatttaggtg ggatgtgatt tcttgaggct
    74101 gggaaaaagt ctttaaaatt aaaaaatgca taatatcagg tgttggaaaa actttgatac
    74161 tatgcatttt attgtgggaa tatttatttc attttctcct gaaattgagt ttttacccag
    74221 cctaaaattc ttgactgact tcgttgaaat ctgtctgctt tggcaagtca tgacacctta
    74281 aaagtctatt tgaatgttcg ttgagacacc gctgtcttgg acatcctaga ttcgcaaaaa
    74341 agctctcact atcgtgatga ttggaaatat ctttccattt tgaaaactct tttccacagt
    74401 caaacgtaat agatttaaag aggttcacta gaaattgtgc cagccaccct tggatggatt
    74461 ctgccatatc ctttgcctta cgactcttgg tcttcaaact gacaatcaat tgactatacc
    74521 tgttcgctca caaataggag agaacttttt acgaaaatct aatgaatatg ccacaagaac
    74581 attataccgc aacgtgtact attttgggtt cattttacta tagaaactat tgaagcaccg
    74641 tacgaaaata tatcatgaaa aaggggagat agaaagcaaa cagccttgaa atcaatgttg
    74701 actgcgatta tgaacctgaa tcagcccctc aaaagggact gtatatgaat gaacgttacc
    74761 agtgtttaaa aactaaagaa tatcaggcac ttttatcttc caagggtaga caaattttcg
    74821 ctaaacgtaa gattgatatg aaatctgtct ttgggcagat aaaggtttgt ttgggttata
    74881 agagatgtca tctgagaggt aagcgtcaag tgagaattga catgggattc atactcatgg
    74941 ccaacaacct gctgaaatat aataagagaa agaggcaaaa ttaaaaagct agagttccgc
    75001 aattgggaat ctctagcttt ttgttttctg agaatcatct tgactcagac tcttttgact
    75061 aatgttacca aaaagataaa gtaactttta accttattta tcgtcgccac tcaacatgtt
    75121 tttaacacct tctacagcac cttctacagc gtctttagcg tcttcggcaa cttcttttac
    75181 tttagaaaca actttttcag ctgctccttc tttttccatt ttttcatcac cgatggcttt
    75241 cccaacacct tctttaatag aacctttagc ttgatttaat ttttcttcta ctgacatgat
    75301 aatttctcct gttatattta ttttatctta gtttacacga gtgttttcgt gagatactac
    75361 accatttgct gcacctttaa cagcttctac accttcgcta actttttctt gaacagttga
    75421 gaaaccagat ccaagaccag atttagcttt ttcgaattgt tctgaagtga attctcctgt
    75481 tgattcagca acgtcagata cgcgatcttg aaggcttact gagtctgctt catgctgttc
    75541 tttagttttg atgtcgacaa cgtttacgtt gatttcaaca atttccaagt cagtcatttt
    75601 agcaacttct gaagatacga tttctctgat ttctgaatat aaagctggaa catttttttg
    75661 gtactcaaca ataacgttta agtcaactgc aacttgtgtt ttaccaactt ctacgttaac
    75721 accacttgtt acgtcatcgc tgttaacgat tttttcttta agatttgaga agaaaccacc
    75781 atcgattccc aaaagacctg aaacgttttc tagtgaaaga ccaatgattt tttggataac
    75841 tttatcttcg taagtaagtt cccctttgat ttcgtgagct acaacagtag catctttctt
    75901 ttctacgtta gtgtttgtgt ttttttcgtt tgacatatga aactccttta attaaattag
    75961 taatttttat ttttttattt atctatatag tttttttcga tatataatcc agctgcaact
    76021 cccagtgctc ctaaaatcaa tacaaatatt gttttgaaga agccaaagga gacaatcaaa
    76081 caagccagaa atacgcctat gagaccagcg ataattggat atcgatattg tttaagccat
    76141 tccatttttt acttccttac ttcacacgac taacagtctt tttgttttga ggttttggtt
    76201 ggtaattttt tactgcaact tcaagtttta cttgacgctc aataccaaaa aactgcttca
    76261 atccattagt tatttcattt tgaattattt ggcatctgtc agcgatgttg tctgaaggaa
    76321 gaattttacc ttctacatga acgaaacatt tattttttcg taaatttaca tgaacagttg
    76381 ggttcttgat caatctatga tcactcacca aacttctaac aaaaccttcg attgccgaat
    76441 tctttaattt taatgtatcg tttttagttt caagttggat ttccaagtaa cgtttaggat
    76501 aaaacatcac aactaaaatg gataataaaa ctaaagttga tagaaccact gtcccccaaa
    76561 agacatatct agcaagatag aattcaacta cggagttctg tctccagcta agtagatgaa
    76621 tacctagatc actaacttga tgataatcta tcaaaacagg aaggaaaatt gtcaagatta
    76681 aaatacagaa aataatgaaa catattttct ttgcttttga catatacaat cctttcgatt
    76741 taacatttaa actatttttc ccgtcattga tttaaaaata gtttgctaac tttactggcg
    76801 aaaagtttac tttctacctg taaagaatga cactacagta atcacaatcg ctgcacctac
    76861 aatagatggg agcaaagcca ttccagcgat tgatggaccc caactaccta aaagagattg
    76921 tcctgcatat gccccgacta aaccagcgaa tacatttgcg atgattccca tagaactact
    76981 tttttagtga ttttacctgc caagagacca ataagacctc cagcaataat tgatcacaac
    77041 actgcctacc ctcctttttt attttaatat caaaaaatca atttctttga ttattactat
    77101 tatataactt taatatagaa aagtcaatta tttcgattgt tattattata taattttaaa
    77161 aaatcaaatt ctttaattgt tgctattata taatttttaa aattaaaaat ataatcttta
    77221 gacttgaatt tgattctgta ttcacgccct caatagacgg taaaataaga aaattgttta
    77281 tatattgtct ccgtagtgtt attatacgaa ataaaagatt ttaggaaaag tcgttatatc
    77341 atgctatacc tattctttgt ggtataattg caagaggttt aatccgataa tttataaaga
    77401 gagaaagata ttcatgagag atttattatc taaaaaaagt cataggcaat tagaattatt
    77461 agaattatta tttgaacata aacgttggtt tcatcgttct gaactagcag agttattaaa
    77521 ttgtacagaa cgtgcagtca aagatgatct atcccatgtt aaatctgctt ttcctgactt
    77581 gatttttcat tcttctacta atggtatacg cattattaat accgatgata gtgatattga
    77641 aatggtttac catcatttct ttaaacattc aactcatttt tcgattttag aattcatctt
    77701 ctttaatgaa ggttgtcaag ctgagagtat ttgtaaagaa ttttatatca gttcatcttc
    77761 gctctatcgt attattagcc aaatcaataa agtgattaaa aggcaatttc aatttgaagt
    77821 cagtctgacc cctgttcaaa tcattggaaa tgagagagac attcgttact tttttgcaca
    77881 atatttttca gaaaaatatt atttcctaga atggccattt gaaaattttt catcagagcc
    77941 actatctcaa ttgttagaat tggtttataa ggaaacaagc tttccaatga atttgtcaac
    78001 gcatagaatg ctaaagttgc tcctagttac gaacctatat agaataaagt ttggtcattt
    78061 tatggaagta gataaagatt cttttaacga ccaaagtttg gattttttaa tgcaggcaga
    78121 aggaatagaa ggtgttgctc agagttttga atcagaatac aatatctctt tagatgaaga
    78181 agttgtttgc caattatttg tgtcttactt tcaaaaaatg tttttcatag atgaaagtct
    78241 ctttatgaaa tgcgtaaaaa aggatagtta tgttgaaaaa tcttaccatc tattgagtga
    78301 ttttattgat cagatttcag tcaagtatca gattgaaatg gagaataagg ataatctgat
    78361 ttggcatctg cataataccg cacatctgta tcgtcaagag ttgtttactg agtttatttt
    78421 atttgatcaa aaagggaata caatcaggaa ctttcaaaat atttttccaa aatttgtttc
    78481 agatataaaa aaagagcttt ctcactattt agagactctt gaggtttgtt ctagttcaat
    78541 gatggtaaat catctatctt ataccttcat cactcatacc aaacatttgg tgattaattt
    78601 gctacaaaat cagcctaaac taaaggtttt ggttatgagt aattttgatc agtatcatgc
    78661 aaaattcgtt gcagagacac tttcttatta ctgtagcaat aattttgaac tggaagtttg
    78721 gactgaatta gaattatcaa aggaatcttt agaagattca ccttatgata tcattatttc
    78781 taattttatt attcctccta ttgaaaataa gagactcatc tattcaaata atataaacac
    78841 ggtctcactc atatatttgt taaatgccat gatgtttatt cgattagatg agtaaccaaa
    78901 aataacaaaa aaacgctatt tcgcgtttga actgtacctc aaaagttaga cagaaaaaat
    78961 ctaatttttg gggtgttttt gttaagaaaa gaccttcaat catttaagca aaatgtacat
    79021 ctagatttat agccatctaa aactcttgct ttttttgtag aagatacgaa aaaagccatg
    79081 aaaccaaggc ttttcctgtt ttatgtagat gtttacacat gaaaattgaa gtattttcaa
    79141 aagctaattg gagtgatttt attcatgatg ggataaatga tctggccaaa aatgattgag
    79201 aaatagttgc ttaaaaagca tttgcatgat actatatatt gaaaggaggt gataatatgg
    79261 tagaacaaag aaaatcaatt accatgaaag atgttgcttt agaagcagga gttagtgttg
    79321 gaactgtttc acgtgtaatt aataaagaaa aaggcattaa agaagtaact ttgaaaaaag
    79381 tggaacaagc gattaaaact ttgaattaca ttccagatta ctatgctaga ggaatgaaaa
    79441 aaaatcgaac agaaacgatt gcaatcattg taccaagtat ctggcatccc ttcttttcag
    79501 aatttgctat gcatgtggaa aatgaagtct ataagagaaa taacaaatta ctcttatgtt
    79561 ctataaatgg tacaaataga gagcaagact atctggagat gttgcgtcat aataaagttg
    79621 atggagtggt tgccattacc tatagtccaa ttgaacatta cttgacgtca ggaattccct
    79681 ttgttagtat tgaccgcaca tactcagata ttgccattcc ttgtgtttca tcagataatg
    79741 atgcaggtgg aagagaagca gctaaacaat taattagtaa aggatgtcag catttagcct
    79801 ttgtgggcgg acataataca accattaatg aaaccaaacg tcgtagaatt tcatttgaaa
    79861 agtatgtcca agagtataaa atacctctta gtatttttga tttggatgag acagttactg
    79921 actattatgg aaagctagag agtttcttgg aagaaaattc ttctatagat ggaattttta
    79981 caatcaacga ttttacagct ttggatgtga ttgaaatact aaaaaaaaat ggcaaacgta
    80041 ttcctcatga tgtccaaatt attggatacg atggaattaa aatggctgga gatagagatt
    80101 atctactttc aactattgaa caaccattgg aagaaatggc aaaagaagcg gttcgtattt
    80161 tgtttgatat aattgatgga aagactgtta atttgcagac aatcctacca gtaaaatttg
    80221 ttgaaggaaa aacaacaaaa aatgaaaata agtcttgaca gaaagcgcta tcaatgatag
    80281 aatgaattca gataaaaaga tttattttta aaacaaaaat gaaacgtttc aaaaaggagg
    80341 ttgctatgaa tagcaaagcg aagcaagttt ctctttggga aagaatcaag aaacaaaaac
    80401 tcttgttatt gatgactgtc cctggtttag ttttaacctt tatctttaaa tacatcccta
    80461 tgtatggggt tttaatcgca tttaaagatt acaatccttt aaaaggaatt ttagggagtg
    80521 attggattgg tttttctgag tttacaaaat tcatatcctc tcccaacttt ggtatcttgt
    80581 tagccaacac attaaaatta agtatctatg gtttattgct tggcttttta ccaccaatca
    80641 ttcttgctat tatgctcaat caactcttga gtgaaaaagt caaaaaacga attcagctca
    80701 ttttatacgc accaaacttt atctcagtcg ttgttattgt cggtatgatt ttcctcttct
    80761 tttcagtggg aggaccaatc aacaattttc tttctatgtt tggaatgaag gttgacttct
    80821 tgacaaatcc agacttcttt agacctttat acatctttag tggtatctgg caaggaatgg
    80881 gctgggcttc aacgctctac acggcaacat tggtaaatgt agatccagcc ttagtagaag
    80941 cagcccgact ggatggagcc aatatcttcc aacgaatctg gcacattgat attccagctc
    81001 ttaagcctat tatggttatc caatttgttt tagctgcagg tggaattatg aatgtcggat
    81061 atgaaaaagc attcttgatg cagacatcgt taaatttgcc aacttctgaa attatctcga
    81121 catatgtcta taaagttggt cttgtatcag gagactattc ttactcaaca gcggttggtt
    81181 tgtttaatgc agtgattaac gtagtattgc ttgttgcagt taaccaaatc gttaaacgca
    81241 tgaataatgg tgaaggaatt taaggaggaa agtatgaaaa attcgattat ggatacaaaa
    81301 tttgatagac gtatcttact cttaaataaa atcattattg tctttatcgt tttgatgact
    81361 ttgcttcctt tactttatat cgtcgtagca tcctttatgg atcctaaggt tctggttagt
    81421 agagggatta gctttaatcc agccgattgg actgtagaag gttaccagcg tgtattcagt
    81481 gaccaatcta ttctaagagg ttttatcaat tctctactat actcttttgg atttgcagct
    81541 ttaacagtct tgctatctgt gtttacagct tatcctcttt ctaagaaaga cttggttgga
    81601 cgtcgttgga ttaactactt cttgattgtg actatgttct ttggtggtgg tttagtccca
    81661 acttacttgc tcgtaaaaga attgggaatg ctcaatactc catgggctat cattgttcca
    81721 ggtgctgtca acgtttggaa tattattctt gctagggcct atttccaagg attgcctgaa
    81781 gaattagttg aagctgctgt cattgatggt gcaaatgatt tacagatttt cttcaaaatc
    81841 atgcttcctc ttgcaaaacc aattatgttt gttctcttcc tttatgcttt tgtaggacag
    81901 tggaactcat actttgatgc aatgatttat atcaaggatc caaacttgga accattgcaa
    81961 cttgtacttc gtaaaattct cattcagagc caaccaggtc aagacatgat tggagcacaa
    82021 gcggctatga atgaaatgaa acgtttagct gaattgatta aatacgcaac tattgtcatt
    82081 tccagcttgc cattgattgt tatgtatcca ttcttccaaa aatactttga taaaggaatt
    82141 atggctggtt cacttaaagg ataaaaaaag aaaaaataaa aggagttttc tcatgaaatt
    82201 caaaacattc tcaaaatcag cagttttgtt gacagctagt ttagcagtac ttgcagcctg
    82261 tggctcaaaa aatacagctt caagtccaga ttataagttg gaaggtgtaa cattcccgct
    82321 tcaagaaaag aaaacattga agtttatgac agccagttca ccgttatctc ctaaagaccc
    82381 aaatgaaaag ttaattttgc aacgtttgga gaaggaaact ggcgttcata ttgactggac
    82441 caactaccaa tccgactttg cagaaaaacg taacttggat atttctagtg gtgatttacc
    82501 agatgctatc cacaacgacg gagcttcaga tgtggacttg atgaactggg ctaaaaaagg
    82561 tgttattatt ccagttgaag atttgattga taaatacatg ccaaatctta agaaaatttt
    82621 ggatgagaaa ccagagtaca aggccttgat gacagcacct gatgggcaca tttactcatt
    82681 cccatggatt gaagagcttg gagatggtaa agagtctatt cacagcgtca acgatatggc
    82741 ttggattaac aaagattggc ttaagaaact tggtcttgaa atgccaaaaa ctactgatga
    82801 tttgattaaa gtcctagaag ctttcaaaaa cggggatcca aatggaaatg gagaggctga
    82861 tgaaattcca ttttcattta ttagtggtaa cggaaacgaa gattttaaat tcctatttgc
    82921 tgcatttggt ataggggata acgatgatca tttagtagta ggaaatgatg gcaaagttga
    82981 cttcacagca gataacgata actataaaga aggtgtcaaa tttatccgtc aattgcaaga
    83041 aaaaggcttg attgataaag aagctttcga acatgattgg aatagttaca ttgctaaagg
    83101 tcatgatcag aaatttggtg tttactttac atgggataag aataatgtta ctggaagtaa
    83161 cgaaagttat gatgttttac cagtacttgc tggaccaagt ggtcaaaaac acgtagctcg
    83221 tacaaacggt atgggatttg cacgtgacaa gatggttatt accagtgtaa acaaaaacct
    83281 agaattgaca gctaaatgga ttgatgcaca atacgctcca ctccaatctg tgcaaaataa
    83341 ttggggaact tacggagatg acaaacaaca aaacatcttt gaattggatc aagcgtcaaa
    83401 tagtctaaaa cacttaccac taaacggaac tgcaccagca gaacttcgtc aaaagactga
    83461 agtaggagga ccactagcta ttctagattc atactatggt aaagtaacaa ccatgcctga
    83521 tgatgccaaa tggcgtttgg atcttatcaa agaatattat gttccttaca tgagcaatga
    83581 caataactat ccaagagtct ttatgacaca ggaagatttg gacaaaattg cccatatcga
    83641 agcagatatg aatgactata tctaccgtaa acgtgctgaa tggattgtaa atggcaatat
    83701 tgatactgag tgggatgatt acaagaaaga acttgaaaaa tacggacttt ctgattacct
    83761 cgctattaaa caaaaatact acgaccaata ccaagcaaac aaaaactaga ggttgattat
    83821 gggagataag aaatacacag tagaaaaagc caatcgtttt atagcagaaa ataaacatct
    83881 cgttaatact caatataagc ctgaagaaca tttttcagct gagattggtt ggatcaatga
    83941 tccaaatgga tttgtctatt ttcgtggaga ataccatctc ttttatcaat tctatccata
    84001 tgatagtgtt tggggaccta tgcactgggg acatgctaaa agtaaggact tggtgacttg
    84061 ggagcacttg ccagtggcac ttgctcctga tcaagattat gaccgaaatg gttgtttctc
    84121 aggctctgcc attgtcaagg atgatcgcct ctggctcatg tacactgggc atatcgaaga
    84181 aaaaaccggt gtccgccaag tgcaaaatat ggcattttca gatgacggga ttcactttga
    84241 aaagattgaa caaaatccag ttgcaacagg aacagacttg ccagatgagt tgattgctgc
    84301 tgatttccgt gatccaaaac tctttgaaaa agatggacgc tattactccg tagtagctac
    84361 caaacacaag gataatgtgg gctgtatcgt tctactaggg tccgataacc tagtagaatg
    84421 gcagttcgaa tccatctttt taaaagggag agaacaccaa ggctttatgt gggaatgccc
    84481 agattacttc gagttagatg ggaaagattg ccttattatg tcacccatgc gttatcagcg
    84541 tgagggagac tcatatcata acatcaactc atcgcttttg ttcacgggta aggtagattg
    84601 gggagaaaaa cgttttatcc cagaatcagt tcaagaaatc gatcatggcc aagacttcta
    84661 tgcgcctcaa acattgttgg acaatcaaaa tcgtcgtatc ctgattgctt ggatgcagac
    84721 atgggggcgt acccttccaa cccatgacca agaacacaaa tgggcatgtg ccatgacttt
    84781 acctagaatt ctaagattgg aagatggcaa actaagacaa ttccctgtta aaaaaggcca
    84841 atatcaaatc caaatagata aagattgtca ttaccactta ggaaatgata tagattatct
    84901 tgaatttggt tatgacagta atgcgcagca agtttacatt gatcgtagcc atcttattca
    84961 aaaaattcta ggtgaagaag aacaggacac tagtcgacgg tatgtagata ttgaagctaa
    85021 agaattggaa gttgttctag ataaaaattc catcgagatt tttgtcaatc aaggtgaagc
    85081 aagcttgact gcaacttatt acttaacggt gccagctgag ctatcacgaa ttgattaaaa
    85141 attaagttat ttctcctaaa gaaaaagttc tctttctaaa atagtggaaa gaggactttt
    85201 tgtgttttgg gtatataagc ttagtttatg gtatttgtaa aattggtgtt ggattatgat
    85261 ttaagctagt tttctaaaga atttgaaaaa aattttattt aagcaaaaaa accttggttc
    85321 caaggctttt cctgttgtat ttagatgccc cctacaggga tcgaacctgt gacccacgga
    85381 ttaagagtcc gctgctctgc cagctgagct aaggaggcaa agaaaaagct gtattggtgc
    85441 cgaaacttca cggtttgtat tgaacccgcg caattaagca ggtgggcaac tcgctctaac
    85501 tgaagctgtt tccgtgtgag acggcctaca tgctgttaga agacttttgt ttccctaata
    85561 atacaaaaaa tagtcggtca acacttaagt gtgaagtcgt acaccacagc gtttctatac
    85621 ttatatgata ccactttttc aaaaaaaatc aagaggaaag tgcaattttt tgaaaaggat
    85681 ttcagatttt tcgcaaacga tcgatagctt cctttctttg agccaaagcc cgttcatatt
    85741 taccagtatc ttctgcttgg aaatagtgat gattacgaat tttttctggc agatagtctt
    85801 gcttgaccca atttccagga tagttgtgtg gataaagata gtcttgggca ttccccagtt
    85861 ccttgcttcc actgtagtgc ccatcacgca ggtgtcgcgg aataggcaag tgccctgatg
    85921 ttttgaggtc agcaagtgcc ttatccatag ctacataggc tgagttggat tttggagaaa
    85981 gggccaaatc aatcacgaca ttggcaatga gaatgcgggc ttcggggaaa ccaatccttt
    86041 gtgcagcatc cagagcagtc acggtatgaa tctgggcctc aggattggct aagccaatat
    86101 cttcataggc gataacagtc aagcgacgag cgagactagg cagatcccca gcctcaatca
    86161 agcgggcagc atagtggaga ctggcatcca catctgagcc acggatagac ttttgcaggg
    86221 cagatagaac atcatagtga ccgtctccat ccttgtccat agtgatgtag cttctctgaa
    86281 gactattttc catgatgtct aaggtgatgt ggcgaatgcc ctcgtcattc tcaggggtag
    86341 agagaacagc caagtccagt gagttaaagg cagagcgaag gtctccgttt gtagaggttg
    86401 cgatgaaatc cagcgcatcc tcatctagtt ctattggaaa atcaaaacca cgttcagggt
    86461 tacttagagc tatctgcagg gcctctttga cgtcttggtt agacagaggt tccaactcaa
    86521 aaatttgaac acggctacga atggcaggag tgacagagaa gaaaggattt tcagtcgttg
    86581 ctccaatcat gatgaccagt ccactttcca agagaggcaa gaggaagtct tgcttggtct
    86641 tatctagtcg atgaatttcg tctagcaata ggacgagacc accagaaaat ttagcttctt
    86701 ccgagatttc ttgcagtcgc tttttactat caactgtcgc attgaaagtt cgaaaggcat
    86761 acttggtcgt tccagcgatg gcagaggcaa tactggtttt gccgattccc ggagggccat
    86821 atagaatcat ggaggacagg cggttggctt ccaccatgcg gcggatgatt tttccaggtc
    86881 cgaccagatg ctcctgaccg atgacctggt cgatggtttt agggcgcatg cgaagcgcga
    86941 gattgtctgg catagcagtc ctttctaacg tggatttgag tagaatgact catctactat
    87001 atcttactat gtccattcta gcctattagt tgtgatattt caatggtttt gtaataatca
    87061 gggagtgaga tagaatcttt tttactattt aatttaatga ggagaggtat cagcatgaga
    87121 gatttgattt tccttaaagc tcaaaatagg atagttgagc ctaaagaagt ggaagtgctg
    87181 acttgagaaa taatattgct gaatgtcatt gtatgaacta taatgaaaag agaggtgata
    87241 agcaatggaa atgatggacc aagtcaggaa attttgattt ttacaaaaca aattcgtcat
    87301 tggattctta gtgatcaagt tatttcaggg aaaagaaaac ttttcttccg ggaagatact
    87361 ccaaaagaga ttttagattt gtacgaaaac attaaatcta aacttgattt tgcttaccaa
    87421 gaagttcatt ctaataatgg gttgaaaaaa tatgaaaaat agaataattg atgtttttga
    87481 agtagtaaat cgcctccttg taataactgt tgaaaatcct gatttcgaag acttgagagt
    87541 taatcatact caatgaaaat caaagagcaa actaggaaac tagtcgcagg ctgtacttga
    87601 gtacggtaag gcgacgttga cgtggtttga atttgatttt cgaagagtat cagtttgtaa
    87661 aaattggaga taaaaaatat agagtacgca gtggtcctat gattcattca actccgcctc
    87721 aatctgtctt agatcgagat acttttacaa ttgactatac agatgatgaa ttgctcgata
    87781 aagaagcagt ttttactacc cattagaaaa ataggtctga ataaattggt ttcccttaga
    87841 accacatcta ggggctttta tgtataatag aataataagt atcactcctt tacaatacgt
    87901 gtaacacgtg ttatcaggag gtaaaagcgc tatacctatg acacaaaaag agatggttaa
    87961 actccttacg gcccatggtt ggataaagac tagaggcggt aaaggctccc atattaaaat
    88021 cgagaagcaa ggggaaagac ctatcacaat ccttcatggt gagctgaata agtacactga
    88081 aagagggata ggaaagcaag ctgggttgta aaattcagtc cctgcttttt gatatggagg
    88141 tagtgaaatg ttagttacgt atccagcttt attttattat gacgacacag acggaacaga
    88201 agcgacttat tttgtccatt tcccagattt tgaatactca gctacacaag gagaggggat
    88261 ttctgaggct ttggctatgg ggtcggagtg gctagggata actgttgcag atttgataga
    88321 gagtgatgga gagttacctc agccatcaga tatcaatagc ttgtctttaa ttgataatga
    88381 tccttttaaa gatgatgaag atttcgtgtc aacctatgac cttgataaat cttttatttc
    88441 tatggtatct gttgatgtat cagaatacct aggaagtcag gaacccatta aaaagacttt
    88501 gaccatacca aaatgggcag ataagttggg acgagaaatg ggacttaact tttctcagac
    88561 tttgacagac gctattgcag ataagaaagt tcaagcctaa gattaagcgt ggtatcgtgg
    88621 taatagtcaa taaaaaagca cgtccacttg tgctagttac tcgcctattg attttataga
    88681 tttattagcc ctttcgaggg ctttttatat tgatttttat gagaaatgaa gaaatcagac
    88741 ttttaagaaa atcactgata ccaagggttt aaatgaggta atatggtata attaggacat
    88801 aaaataattt tgtggtaaga tggtagtatc tattttagca tatttccgag caatcggggc
    88861 gattaaagag tcgcatagaa agaggacaaa atggcaacat acggattttt agatatttta
    88921 gaggaagagt tggacaagaa ctttcccttt gactttgaga ttagttggga caagcgtaac
    88981 cacgcggtag aagtgagttt tctattggaa gcgcaaaatg ctgcaggtgt ggagatggtg
    89041 gatgaagacg gagaggtttc atcagatgac attctttttg aggaagcggt ccttttctac
    89101 aatcctgcca agtcaaccgt caatgcagaa gactatttaa cggttatccc ttatctacct
    89161 aaaaaaggat tttctcgcga gtttttagct tattttgccc ttttcctcaa agatactgcg
    89221 gaggttgggc tagataccct catggacttt ttggaagatc cagaagcaga ggaatttgtt
    89281 atggaatgga atcaagaagt ctttgaagaa ggaaaagttg gcttggaaga gggagaattt
    89341 tatccttatc caagatacta ggagttggtt ggaggtgtta tgaagaaaat tgggacttat
    89401 ttggtttatg tgctagctgt tgtctttatt atgctagctt ttgcttgtgg aacaatcgca
    89461 tttgcagagt tggggtattc cgcagtttta gcctttactt ttggttatgc cttcgctctt
    89521 ctaagcatgt atttaatctt tattcttcat gagctgggtc atgctttttg tggctacttg
    89581 acaggctatc ggctggtggc ttttggatta ggacatttta ttttgaccaa aaagtcaggc
    89641 aggtttcatc ttagtagaac agccattctg aaaaatgttg gtgctcaata cattggttta
    89701 aaagaggatg aaagcgatca aagaatcatc ctgatgcttt caggaggctt gatggttcat
    89761 ctcggcttga tattattggc gatagtgttt ggatttttga caagaagctg gtattttgca
    89821 gggacttgga tttttcttaa tttatctttc ttcctaaata acattttgcc agtcgacatc
    89881 accgatggag caaaaatttg ggaattacta caacaccctg aaaataccaa atacgcctac
    89941 ctggtattga gacattctgc tcaaactttg ctggctcctc aagaatatga tttgaaagac
    90001 tttgttcaag cagttcctga ggatgcacga gggagttttg cggatggtgt attaggtatg
    90061 caaggtgaag tttccatttt ggaagggaag gaagaagtag ctaaacagca gtttcaagca
    90121 ttacttgaga ggacaactac tccgatgatg cagacagttg cccaactatc cctgcttcat
    90181 atcgccttgt tggaaggaga ttttgagcaa gcggagcaat atgccagtat tcgacaggtc
    90241 aaatcgtttc tgtctctcaa actgtccaat cttcaagtga tacaagcctg gtatcaatta
    90301 aaggtgaaga aagatgtaga tcgaactcgt aaggctctca agattgctag acagaaaatg
    90361 gatagtagtc gaatgttgag ggacgagaaa cgctattatg aaaacttgtt agcagagctg
    90421 gaaaaggaat tagccgaagg agtctaatat ggaaatagag atttctgatt tcacaggttg
    90481 caagattgct ttgttttgtg gggataagct tttgactatc ttacgcgatg ataaggcaag
    90541 cattccctgg gccaatatgt gggaactgcc aggtggggga aagagtgtca gggaaaatct
    90601 gatatagttt tgcattaaaa gaatatcctt tcaaggttat tcttttttaa ttttgtccca
    90661 aaataattga ctttgtcccg aaaatatagt attatttgta taaggaggaa tgaatcatga
    90721 aaaaatcaga tgttcttgat ttaataaagt atcattatga gggcagagag acagaattta
    90781 gaaatcaatc catagcaatt gctcggaact tcaataaaca tggtgataca caaatagcac
    90841 aatatattat ggggttaatg tctcaaagtg atagattcat gccacaaata gaaaatccta
    90901 gtgagtattt gactcctgct aaactagata ttggtcctct cccattgcct ctttcaatta
    90961 tgaatgattt aaaaggaata ataaacgctg tcaatcatca tattggaata aataaatttt
    91021 tatttgtggg atctccaggt actggaaaaa ctgaaagtgt aaagcaggtg gctagactta
    91081 ttggcaagga attacttgta gtagatttga gtcacttagt agatagtaag ctagggcaaa
    91141 cagtcaaaaa cttagcaact ctttttaatg aaatcaataa tcttcctttc aagcaaaatt
    91201 atatcatcct atttgatgaa attgattcca tagtgttgga tagggttaat cagaatgatt
    91261 taagagaaat gggacgagtg acttctgcct ttttaaagga gttggatagg ctgtcaccag
    91321 aaattgtatt aattgcaaca acaaatcttt ttgaaaatct tgataaagca gttacgagaa
    91381 gattcgatgc cataattgac tttgaccgtt atactgatga agataaagta gaagtagcta
    91441 ctattatttt aaatgagtta ttaaagcaat ttaagaatgt agctagagat ttaaaattat
    91501 ttaaaaaaat tatcaatagt gccaatgtaa taccgaatcc tggtgatttg agaaactcaa
    91561 taagaacgtc attggcattt agtgacccat cagatccgca tgattaccaa aaacgcctgc
    91621 taagaagttt gcataatggt agaaatttat ctatttctaa attatcgaag ctaggtttta
    91681 ctgttagaga aattgagatt ttgacaggta tttctaagag tagtgtatca cgagagttaa
    91741 gcgaggatta aaatgaatac attattgaca ttacgaggta aaagttttac accaaagtca
    91801 gcaaagggga ggggaccgat aacgattccc gcagattcta ttgttacgct aaaccattta
    91861 aatgaacttt atacatccct tgaacgagta aaagaatatt ggtctaataa cacaatcatt
    91921 gatggagtac ttattagtgt gtattataat cgtgtcctag ctaagagcaa tcgtatcgat
    91981 ggttttttta atgtgggtgg cggaaatgca attccaaatg aaacgattgt gggtgcaaaa
    92041 tttagtaatg ataagagtag gcatattatt acacactaca tttcaaagga agtgttagat
    92101 aagacaattg agcatactaa aaaagttata acaatattca aagattattt tgctagtgct
    92161 cccatgacga gtatggtgtt ctccgactct tcaaaattta atcaaattga ttattctaag
    92221 tatggtctgt cgaaatcaaa attcaaacaa tacttaagag acgcttgctt tgtcaaagga
    92281 tttggtattg aacaggcaga tatgtctact atacgtaatt ctattgtaac aatctacgat
    92341 gtaaaaacta atctcttaac attacttaaa caacttgata ttgatgtttc agaagctaac
    92401 atcatggacc agacaactgt tttacttgac gaaaaaaata ttgagttact actgctaaaa
    92461 gcaccttatc tagtatcaat gatagtagaa gatttttcta gcctgtcacc agagaatttt
    92521 tataatgatg ggaacacttt ttataataat ctcccttttc caactaatga acctattatt
    92581 ggtgtcatcg atacgttgtt tgatgatgga gtttatttca atgagtgggt agagtatcat
    92641 gatttaattt caccagatat tagcaaagga agtcaagact ataagcacgg tacagcagtt
    92701 tcctcggtaa tagtagatgg agctagtttg aatccaaaac tagatgacgg atgtggaaga
    92761 tttagagtca gacattttgg gatatcttta cagagtggtt tcaattcctt cacaattatc
    92821 aaacagatta gagaaattgt gtctcaaaat cctgatatta aggtttggaa cctttcgctt
    92881 ggatcaaatg atgaaattag ggaaaacttt atttccgtcg agggttcgtt gttagacgag
    92941 attcaatttg ataacgatgt aatttttatt attgcaggca ctaatgggat tgcctctgat
    93001 ggacaacgaa aaaagattgg agcaccagca gattcactca attcaattat tgtaaattct
    93061 gttgatttta gcaatcaagt cgtttcttat tcacgcgaag gagttatcct gtctttcttt
    93121 atcaaacctg atgtttcata ctatggcggg gggaacggag acttcataaa tgtttgtgaa
    93181 ccactagggc tggctcgagt agctgggacc agttttgcgg ctccatttat tgctagaaaa
    93241 atggcttacc tcattcatat tatgggatta agtcgtgaag aagcaaaggc tttattaatt
    93301 gattcagcga taccatggaa tgataaaaaa acattcgcag acatttcttt acttggatat
    93361 ggggttgtgc caatcaaaat agaagatatt ttgtctacac ctgatgatga aattaaattt
    93421 atagtctcag atgtttctag agcatatgaa acttataatt atgatttccc agttccaatt
    93481 tctaaagaac actatccata tgtagcaaag gccactatgt gctattttcc aaagtgttct
    93541 agaaatcaag gagttgacta tactaatacg gaaatgcaac ttagtttggg agttttagat
    93601 aagaaaggca ttaaaacaat taataacgac aatcaatatt ctgaaaatgc accaggatat
    93661 gtgagagaaa agtcggcacg aaatattttt aggaaatggg ataatgtcaa acatttagga
    93721 gaaaaatttt cttctcgtaa aaaagctaag ccgattctga atccttctaa tcctcaatgg
    93781 ggaatgagtg taaaaacaat tgaacggtta agaaatgatg atggttatgg tattcgtttt
    93841 ggagtagttg ttactctaaa ggagttgaat ggtgtcaata gaatcgaaga ttttattcaa
    93901 caagccgaat tacgcggttg gctagttaat agattgcaag tagaagctca agtagacctc
    93961 tttaatagtt taaatgaaga aattgaattt gaatagtgga tattttatcc ttattctaga
    94021 tcttaggaac acaagagggc aaagatatga atattactta tatagttgga aatggtttgg
    94081 atttgcaata tggtttgaaa acaagatata aagatttcta tgaatttcaa aataggactt
    94141 acataagtag gaaagagaac gaggaagaat attcaaatct catttatgag tctctatttt
    94201 ctgatacagt aaatgattat gaaaattggt cagattttga attgtcaatt ggtaaattaa
    94261 caaaagataa cgacctcatc tcctcaagta tagaaataaa ggagaaattt atagatgatt
    94321 ttagtgaagt agttgatgat ttaagagaat atctacgcat acaacaagaa aaaaatcttg
    94381 agaaaggtaa tgcaattgac tttataagca ctctagataa tatgaggacc tctctccctg
    94441 taattaatca acctgctatc gataaaaaat ataatgaaaa tctatatcaa cacgatatcg
    94501 tgaatatatt aactcttaat tacacaaatg taatcgataa attatataat gagtcagcaa
    94561 aatcatttag caatcaatta cgaactaata attataaatt tcacattgcg cctccaattc
    94621 atgcacatgg aacggtagat atttgcacag ttttaggagt tagtgacgag acacagattt
    94681 caaatagttt tgatgaagaa caaaaagaat ccttaataaa aaatttagtt ttgaagaact
    94741 atagagaaaa tatggatgtt aaaaatagtg atattataaa aaattctgac atcattattc
    94801 tctatggggt ttctttggga gaaactgata ggtatatctg gagtcagatt gctgaacgat
    94861 caatcagcgg ttctgttcct gtcataattt accactatgt acctcatttt gatccgggga
    94921 atcccattag agcaaaacgc ctatatagaa atgtagaaga taaattcata cagaatagtg
    94981 gtatagactt agagttagag aaaaaattaa gagacaacct tattgtcgtt attggaaaga
    95041 ctatttttaa tctgatggaa aggtaagcaa aatacataat ggaaacatgg caagagttaa
    95101 aagttacagt gaagcgtgag ggagaggagt tggtttccaa tctcttgatt gagctgggag
    95161 cgcaaggtgt tgcgattgaa gacagtatgg actatgtggg aaatgttgac cgctttggcg
    95221 agattttccc agaggtggag caacaggaag aaatcgttgt gacagcctac taccctgaca
    95281 cggtggatgt gacagtggtt gaggcggact tgcaggctcg catatcagaa ttgacggatt
    95341 ttatggattt gggagagtta aaaataggta cgactgcctt ggctgaggaa gactgggcag
    95401 acaactggaa gaagtattat gaaccggcac gcatcaccca tggcctgacc atcgtaccgt
    95461 catggacgga ctatgaggca acagcaggag aaaagattat caagctggat cctggcatgg
    95521 cttttggtac tggaacccac ccaactacca agatgagcct ttttgccttg gaacaggttc
    95581 ttcgtggtgg cgaaacggtg ctagatgtgg ggactggttc aggggttctc tctattgcca
    95641 gctcgcttct gggggctaaa gaaattttcg cctatgacct ggatgatgtg gcggttcgtg
    95701 tggctcagga aaatattgag ctcaaccctg gcatggaaaa tatccatgta gcggatggag
    95761 atttgcttaa gggtgtggaa attgaggcag atgtgattgt ggctaatatc ttggcggata
    95821 tcctcattca tctaatagac gatgcctatc gtttggttaa ggacgaaggc tacctgatca
    95881 tgagtggcat tatcaaggat aagtgggaca tggtgcgcga gtcggctgaa tcagctggat
    95941 ttttcctcga aactcacatg gttcaagggg aatggaatac ctgtgtcttt aagaaaacca
    96001 aggatatctc tggtgtgatt ggaggctagc atgcagcagt attttgtaaa aggcagtgct
    96061 atctctcctg tcactatcga ggacaaggaa accagtaagc atatgtttca ggttatgcgc
    96121 ttgaaagaag aggatgaggt taccttagtc tttgatgatg gcatcaagcg cttggcgcgc
    96181 gtgctggata tggaaaatcg tcagtttgag ttggtccaag aattaaatga caatgtggaa
    96241 ctaccagtcc aagtgaccat cgcatctgga tttcccaagg gagacaagct ggagttcatc
    96301 gctcaaaaag taaccgaact gggtgctagc caaatctggg cctttcctgc agactggtca
    96361 gttgccaagt gggatggcaa gaaattgggt aaaaaagttg aaaaactaga aaaaattgcc
    96421 cttggagcag ccgagcaaag caagcgtaat attgtaccaa gtattaaact tttcgagaaa
    96481 aaagcagatt ttctagctca gctggaccag tttgactcta tcatagtagc ctatgaagaa
    96541 tcagctaaag aaggagaagc cgctgcgctt ctgcaagcag tcactggtct tgaaacagga
    96601 gccaaactgc tctttatctt tggtccagaa ggcggtctct cacctgcaga aatcgaaagt
    96661 tttgaagcta agggagccgt tttggtaggc ctaggtcctc gtattttgcg agcagaaaca
    96721 gcaccgcttt acgccttatc atcccttagt gttttagtag aattagagaa ataagaggaa
    96781 gaaaatggaa caaaaacacc gttcagaatt tccagagaag gaactctggg acttaacagc
    96841 cctataccaa gaccgtgagg atttcttgcg tgcaatcgag aaagctcgcg aagacatcaa
    96901 ccagtttagc cgtgattaca agggcaatct tcacactttt gaggatttcg agaaggcctt
    96961 tgcggaattg gaacagatct acattcagat gagccatatt ggcaactatg cttttatgcc
    97021 tcagacgacg gactatagca atgacgaatt tgcaaatatt gctcaagctg ggatggaatt
    97081 tgaaacagat gccagcgtag ccttgacctt ttttgacgat gccttggtgg cagcagacga
    97141 ggaagtcttg gaccgtttgg gtaaattgcc acatttaaca gctgccattc gtcaggctaa
    97201 aatcaaaaaa gcccactact taggggcaga tgtggagaag gccttgacaa atctcggtga
    97261 agttttctac agtccgcagg acatttatac taagatgcga gctggggatt ttgaaatggc
    97321 tgactttgaa gcccatggca agacctacaa aaacagcttt gtgacctatg agaatttcta
    97381 ccaaaaccat gaggatgctg aggttcgtga gaaatccttc cgttccttct cagagggact
    97441 tcgtaagcac caaaatacgg ctgcagcagc ctatctggct caggtcaagt ctgaaaaact
    97501 cttggctgat atgaagggat acgactctgt ctttgactat cttctagctg aacaagaagt
    97561 ggaccgtgtc atgtttgacc gccagattga cctcatcatg aaggactttg caccagtcgc
    97621 tcagagatac ctcaagcatg ttgccaaggt aaatggtctt gaaaagatga cctttgcaga
    97681 ctggaaattg gacttggaca gcgccctgaa tcctgaagtg actattgacg atgcctatga
    97741 tttggtcatg aagtcggtag aacctttggg gcaagaatat tgtcaggaag ttgctcgtta
    97801 ccaagaagag cgctgggtgg actttgctgc taacagtggc aaggattccg gtggttatgc
    97861 ggcggaccca tatcgcgtac acccttatgt actcatgagc tggacaggcc gtttgagcga
    97921 tgtctatacc ttgattcatg aaatcgggca ttctggtcaa ttcatctttt cagacaatca
    97981 ccaaagctat ttcaatgccc acatgtcgac ctattatgtt gaagcaccgt caaccttcaa
    98041 tgaattgcta ctcagtgatt acttagagaa ccagtctaat gacccacgtc aaaaacgctt
    98101 cgctctggct catcgcttga cagacaccta cttccataat ttcatcaccc acctcttgga
    98161 agccgccttc cagcgtaagg tgtatacatt gattgaagaa ggggagacct ttggagcaag
    98221 caagctcaac agcattatga aggaagtttt gacggatttt tggggcgatg ccattgaaat
    98281 cgacgacgat gcagctttga cttggatgcg ccaagctcac tactacatgg gcttgtatag
    98341 ttacacttac tcagcaggac tagtcatctc gactgcaggt taccttcatc ttaaaaactc
    98401 agaaactgga gctgaagact ggctcaatct cctcaaatca ggtggtagca agacaccact
    98461 tgagtcagcc atgattatcg gtgctgatat ttcaacagac aaaccactcc gtgataccat
    98521 ccaattcttg tctgacacag ttgaccagat tatcgcctac agtgcccagt tgggagagta
    98581 aggggaatag aaaaattttc tagaatgatg tttatgggct tgttaagcgt cattctattt
    98641 gtcttagttg tagctatttt caaaaatgtc gttgaaggtc gtgatgttaa atttgaactt
    98701 actgtagaac tggtattgat agttatttac gtcattttat atcagattgt atttaattga
    98761 gaattcaata ttgagtcatt taagactgcc tttttgaaca gttgctagaa atgtagaggt
    98821 attgaccatg tatcaagatt tactaagaaa aatagcagaa gaaaaaccaa attataacca
    98881 ggaagaaatc cagtggttgt ttgatcattt gggaaatcct tctccagaaa ttcgtaatgt
    98941 ccttttaaat caaggcttgc actatctttc aaaagaaaag gatacaacag gtttctcaag
    99001 tcagtatggt tgggttcacg cttttgcaca tggagccgat ttactgacag aggtggtttg
    99061 tcatccagac tttcctaaaa acagagttca tgaagtattt gatatacttg gccaactatt
    99121 taaaagaatg tcgattcgct ttacagatga tgaggattgg cgtttagcaa gagtgatcta
    99181 tgaacctatt ttacaaggga agttggagca agaacaagta gcttcttgga taaaaactgt
    99241 tgattttccg atagaagaaa gggaggattt ttacaaattt tccaacttta gatcctgtct
    99301 ggtggaagtc tatgtccaac ttgaccagag aaatagttta caagatgagt tgaaagaagc
    99361 tatccagtct tttcaatact agagattagc taatagttct atgattttca aaaaatttcc
    99421 aatcaatttt cttgaaaccc tttcctactt ttgatagact agtatctagt ttttgaaaaa
    99481 aggagaaaga aaatgaaaaa atttgttgct gagttaatcg gtacgttcat gcttgtgttc
    99541 gttggaacag gagctgttgt ttttggaaat ggtcttgatg gccttggtca ccttggaatc
    99601 gcctttgcct ttggtttggc aatcgtggtg gcagcctact caatcggaac tgtttcaggt
    99661 gctcacttga acccggctgt ttcgattgct atgtttgtaa acaaacgttt gtcatcttca
    99721 gaacttgtaa actacatcct tggtcaggtt gttggagctt tcatcgcttc tggcgcagtc
    99781 ttcttcctct tgagtaattc aggtatgtca actgctagtc ttggtgaaaa tgccttggca
    99841 aacggtgtca ctgtctttgg tggtttcttg tttgaagtca tcgcaacttt cttgtttgta
    99901 ttggttatca tgactgtgac ttcagaaagc aagggcaatg gcgcgattgc tggtttggta
    99961 atcggtttgt cattgatggc gatgattctt gtcggattga agattactgg actttcagta
   100021 aacccagctc gtagcttggc accagctgtc ttggtaggcg gcgcagccct tcaacaagtt
   100081 tggattttca tccttgcacc aatcgctggt ggagttcttg cagcccttgt tgcaaaaaat
   100141 ttccttggaa cagaagaata attgaaactc aaaaagcctt gctcctcatc ttgaggaaca
   100201 gggctttttc gtatgatact cttcgaaaat ctcttcaaac cacgtcagct ttaccttgcc
   100261 gtaggtatgg tt
//
LOCUS       DAVDWM010000003        90839 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVDWM010000003 DAVDWM010000000
VERSION     DAVDWM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 90839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.003"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(385..777)
                     /gene="rpsI"
                     /locus_tag="VUW49_000217"
     CDS             complement(385..777)
                     /gene="rpsI"
                     /locus_tag="VUW49_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7980196.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(797..1243)
                     /gene="rplM"
                     /locus_tag="VUW49_000218"
     CDS             complement(797..1243)
                     /gene="rplM"
                     /locus_tag="VUW49_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7980197.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1600..1917
                     /locus_tag="VUW49_000219"
     CDS             1600..1917
                     /locus_tag="VUW49_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET7980198.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1962..2774)
                     /gene="folK"
                     /locus_tag="VUW49_000220"
     CDS             complement(1962..2774)
                     /gene="folK"
                     /locus_tag="VUW49_000220"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7980199.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFIVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2817..3371)
                     /gene="folE"
                     /locus_tag="VUW49_000221"
     CDS             complement(2817..3371)
                     /gene="folE"
                     /locus_tag="VUW49_000221"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7980200.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVIIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3352..4674)
                     /locus_tag="VUW49_000222"
     CDS             complement(3352..4674)
                     /locus_tag="VUW49_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7980201.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGSIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRVYLMERKNENGYTKD"
     gene            complement(4676..5650)
                     /gene="folP"
                     /locus_tag="VUW49_000223"
     CDS             complement(4676..5650)
                     /gene="folP"
                     /locus_tag="VUW49_000223"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7980202.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMPHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5745..6452)
                     /locus_tag="VUW49_000224"
     CDS             complement(5745..6452)
                     /locus_tag="VUW49_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980203.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIIPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6506..7924)
                     /locus_tag="VUW49_000225"
     CDS             complement(6506..7924)
                     /locus_tag="VUW49_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET7980204.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            8114..8926
                     /locus_tag="VUW49_000226"
     CDS             8114..8926
                     /locus_tag="VUW49_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7980205.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKNPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9265..10284
                     /gene="adhP"
                     /locus_tag="VUW49_000227"
     CDS             9265..10284
                     /gene="adhP"
                     /locus_tag="VUW49_000227"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET7980206.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAIVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            10517..11506
                     /gene="manX"
                     /locus_tag="VUW49_000228"
     CDS             10517..11506
                     /gene="manX"
                     /locus_tag="VUW49_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET7980207.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            11534..12337
                     /locus_tag="VUW49_000229"
     CDS             11534..12337
                     /locus_tag="VUW49_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET7980208.1"
                     /translation="MSIISMVLVVGVAFLAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPATLL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            12361..13272
                     /locus_tag="VUW49_000230"
     CDS             12361..13272
                     /locus_tag="VUW49_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET7980209.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13328..14662)
                     /gene="pepC"
                     /locus_tag="VUW49_000231"
     CDS             complement(13328..14662)
                     /gene="pepC"
                     /locus_tag="VUW49_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET7980210.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTSVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(14926..15648)
                     /locus_tag="VUW49_000232"
     CDS             complement(14926..15648)
                     /locus_tag="VUW49_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET7980211.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVEIFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQVKRMVAYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRLLTKEELEILRANII"
     gene            complement(15916..16146)
                     /locus_tag="VUW49_000233"
     CDS             complement(15916..16146)
                     /locus_tag="VUW49_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7980212.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(16156..17397)
                     /locus_tag="VUW49_000234"
     CDS             complement(16156..17397)
                     /locus_tag="VUW49_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7980213.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(17419..17571)
                     /locus_tag="VUW49_000235"
     CDS             complement(17419..17571)
                     /locus_tag="VUW49_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980214.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(17592..17870)
                     /locus_tag="VUW49_000236"
     CDS             complement(17592..17870)
                     /locus_tag="VUW49_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET7980215.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAESLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(17863..18126)
                     /locus_tag="VUW49_000237"
     CDS             complement(17863..18126)
                     /locus_tag="VUW49_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET7980216.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(18228..22619)
                     /locus_tag="VUW49_000238"
     CDS             complement(18228..22619)
                     /locus_tag="VUW49_000238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET7980217.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDVQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     GAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(22723..24804)
                     /gene="fusA"
                     /locus_tag="VUW49_000239"
     CDS             complement(22723..24804)
                     /gene="fusA"
                     /locus_tag="VUW49_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7980218.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(25229..25699)
                     /gene="rpsG"
                     /locus_tag="VUW49_000240"
     CDS             complement(25229..25699)
                     /gene="rpsG"
                     /locus_tag="VUW49_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7980219.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(25719..26132)
                     /gene="rpsL"
                     /locus_tag="VUW49_000241"
     CDS             complement(25719..26132)
                     /gene="rpsL"
                     /locus_tag="VUW49_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7980220.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            26549..30391
                     /gene="spuA"
                     /locus_tag="VUW49_000242"
     CDS             26549..30391
                     /gene="spuA"
                     /locus_tag="VUW49_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET7980221.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            complement(30491..31540)
                     /locus_tag="VUW49_000243"
     CDS             complement(30491..31540)
                     /locus_tag="VUW49_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7980222.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLTAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            31597..31734
                     /locus_tag="VUW49_000244"
     CDS             31597..31734
                     /locus_tag="VUW49_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980223.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            complement(31811..33619)
                     /gene="glmS"
                     /locus_tag="VUW49_000245"
     CDS             complement(31811..33619)
                     /gene="glmS"
                     /locus_tag="VUW49_000245"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7980224.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEKYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKRALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(33811..35190)
                     /gene="bglA"
                     /locus_tag="VUW49_000246"
     CDS             complement(33811..35190)
                     /gene="bglA"
                     /locus_tag="VUW49_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET7980225.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(35289..37142)
                     /locus_tag="VUW49_000247"
     CDS             complement(35289..37142)
                     /locus_tag="VUW49_000247"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7980226.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(37155..38414)
                     /gene="rseP"
                     /locus_tag="VUW49_000248"
     CDS             complement(37155..38414)
                     /gene="rseP"
                     /locus_tag="VUW49_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET7980227.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(38436..39239)
                     /locus_tag="VUW49_000249"
     CDS             complement(38436..39239)
                     /locus_tag="VUW49_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7980228.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(39248..40006)
                     /locus_tag="VUW49_000250"
     CDS             complement(39248..40006)
                     /locus_tag="VUW49_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7980229.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(40237..40809)
                     /locus_tag="VUW49_000251"
     CDS             complement(40237..40809)
                     /locus_tag="VUW49_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7980230.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLKKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(40790..41761)
                     /gene="ruvB"
                     /locus_tag="VUW49_000252"
     CDS             complement(40790..41761)
                     /gene="ruvB"
                     /locus_tag="VUW49_000252"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7980231.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            41922..42014
                     /locus_tag="VUW49_000253"
     CDS             41922..42014
                     /locus_tag="VUW49_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET7980232.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(42829..43245)
                     /locus_tag="VUW49_000254"
     CDS             complement(42829..43245)
                     /locus_tag="VUW49_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980233.1"
                     /translation="MITIRKQEIVKFEDVLHLYQAVGWTNYTNQPQMLEQALYHSLVI
                     YLALDGDAVVGLIRLAGDGFSSVFVQDLIVLSSYQRQGIGSSLMKQALGNFKEAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(43257..43952)
                     /locus_tag="VUW49_000255"
     CDS             complement(43257..43952)
                     /locus_tag="VUW49_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980234.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELTFIDLLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMEELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLLSRRAAERLGFIYEGTFRQAVVYKGCTRDTDWLSMIDKDWPKVKDRLETWLRPENF
                     DKNGQQYKSLREF"
     gene            complement(44252..46753)
                     /locus_tag="VUW49_000256"
     CDS             complement(44252..46753)
                     /locus_tag="VUW49_000256"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7980235.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDTSKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            complement(47208..48296)
                     /locus_tag="VUW49_000257"
     CDS             complement(47208..48296)
                     /locus_tag="VUW49_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET7980236.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(48314..48982)
                     /locus_tag="VUW49_000258"
     CDS             complement(48314..48982)
                     /locus_tag="VUW49_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET7980237.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(49001..51439)
                     /locus_tag="VUW49_000259"
     CDS             complement(49001..51439)
                     /locus_tag="VUW49_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET7980238.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGRFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(51590..52888)
                     /locus_tag="VUW49_000260"
     CDS             complement(51590..52888)
                     /locus_tag="VUW49_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7980239.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLACQNEIKQS"
     gene            complement(52905..53213)
                     /locus_tag="VUW49_000261"
     CDS             complement(52905..53213)
                     /locus_tag="VUW49_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7980240.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(53259..53579)
                     /locus_tag="VUW49_000262"
     CDS             complement(53259..53579)
                     /locus_tag="VUW49_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7980241.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(53800..54753)
                     /locus_tag="VUW49_000263"
     CDS             complement(53800..54753)
                     /locus_tag="VUW49_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET7980242.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGI"
     gene            complement(54766..55512)
                     /locus_tag="VUW49_000264"
     CDS             complement(54766..55512)
                     /locus_tag="VUW49_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7980243.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            55634..56410
                     /locus_tag="VUW49_000265"
     CDS             55634..56410
                     /locus_tag="VUW49_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET7980244.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            56485..56682
                     /locus_tag="VUW49_000266"
     CDS             56485..56682
                     /locus_tag="VUW49_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7980245.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            56899..57495
                     /locus_tag="VUW49_000267"
                     /pseudo
     CDS             56899..57495
                     /locus_tag="VUW49_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            57580..58020
                     /locus_tag="VUW49_000268"
     CDS             57580..58020
                     /locus_tag="VUW49_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7980246.1"
                     /translation="MLVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDTAVKLLNDGAN
                     IKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGATTTNRPVRKNA
                     KTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            58033..59124
                     /locus_tag="VUW49_000269"
     CDS             58033..59124
                     /locus_tag="VUW49_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980247.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACTLAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIRAIQHEVGITTVYVTHDQEEAMAISDQIAAMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPTNLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            59114..60749
                     /locus_tag="VUW49_000270"
                     /pseudo
     CDS             59114..60749
                     /locus_tag="VUW49_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(60852..61544)
                     /locus_tag="VUW49_000271"
     CDS             complement(60852..61544)
                     /locus_tag="VUW49_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7980248.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(61788..63125)
                     /locus_tag="VUW49_000272"
     CDS             complement(61788..63125)
                     /locus_tag="VUW49_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET7980249.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGTSSVDFISRGGQIPAPIHSFKN"
     gene            complement(63135..63401)
                     /locus_tag="VUW49_000273"
     CDS             complement(63135..63401)
                     /locus_tag="VUW49_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7980250.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(63666..64052)
                     /gene="rplQ"
                     /locus_tag="VUW49_000274"
     CDS             complement(63666..64052)
                     /gene="rplQ"
                     /locus_tag="VUW49_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7980251.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(64064..64999)
                     /locus_tag="VUW49_000275"
     CDS             complement(64064..64999)
                     /locus_tag="VUW49_000275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7980252.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(65042..65425)
                     /gene="rpsK"
                     /locus_tag="VUW49_000276"
     CDS             complement(65042..65425)
                     /gene="rpsK"
                     /locus_tag="VUW49_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7980253.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(65443..65808)
                     /gene="rpsM"
                     /locus_tag="VUW49_000277"
     CDS             complement(65443..65808)
                     /gene="rpsM"
                     /locus_tag="VUW49_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET7980254.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDVIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(65826..65942)
                     /gene="rpmJ"
                     /locus_tag="VUW49_000278"
     CDS             complement(65826..65942)
                     /gene="rpmJ"
                     /locus_tag="VUW49_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET7980255.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(65967..66185)
                     /gene="infA"
                     /locus_tag="VUW49_000279"
     CDS             complement(65967..66185)
                     /gene="infA"
                     /locus_tag="VUW49_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7980256.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(66302..66940)
                     /locus_tag="VUW49_000280"
     CDS             complement(66302..66940)
                     /locus_tag="VUW49_000280"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7980257.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAQALDKTLA
                     ELGIELEGVINIEVNPNSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(67091..68401)
                     /gene="secY"
                     /locus_tag="VUW49_000281"
     CDS             complement(67091..68401)
                     /gene="secY"
                     /locus_tag="VUW49_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7980258.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(68414..68854)
                     /gene="rplO"
                     /locus_tag="VUW49_000282"
     CDS             complement(68414..68854)
                     /gene="rplO"
                     /locus_tag="VUW49_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7980259.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(68999..69223)
                     /gene="rpmD"
                     /locus_tag="VUW49_000283"
     CDS             complement(68999..69223)
                     /gene="rpmD"
                     /locus_tag="VUW49_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7980260.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSFVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(69195..69689)
                     /gene="rpsE"
                     /locus_tag="VUW49_000284"
     CDS             complement(69195..69689)
                     /gene="rpsE"
                     /locus_tag="VUW49_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7980261.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(69707..70063)
                     /gene="rplR"
                     /locus_tag="VUW49_000285"
     CDS             complement(69707..70063)
                     /gene="rplR"
                     /locus_tag="VUW49_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7980262.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(70147..70683)
                     /gene="rplF"
                     /locus_tag="VUW49_000286"
     CDS             complement(70147..70683)
                     /gene="rplF"
                     /locus_tag="VUW49_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7980263.1"
                     /translation="MSRIGNKVIVLPAGVELTNNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(70875..71273)
                     /gene="rpsH"
                     /locus_tag="VUW49_000287"
     CDS             complement(70875..71273)
                     /gene="rpsH"
                     /locus_tag="VUW49_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7980264.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(71558..71827)
                     /gene="rpsN"
                     /locus_tag="VUW49_000288"
     CDS             complement(71558..71827)
                     /gene="rpsN"
                     /locus_tag="VUW49_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET7980265.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(71845..72387)
                     /gene="rplE"
                     /locus_tag="VUW49_000289"
     CDS             complement(71845..72387)
                     /gene="rplE"
                     /locus_tag="VUW49_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET7980266.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(72411..72716)
                     /gene="rplX"
                     /locus_tag="VUW49_000290"
     CDS             complement(72411..72716)
                     /gene="rplX"
                     /locus_tag="VUW49_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET7980267.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(72794..73162)
                     /gene="rplN"
                     /locus_tag="VUW49_000291"
     CDS             complement(72794..73162)
                     /gene="rplN"
                     /locus_tag="VUW49_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET7980268.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(73188..73448)
                     /gene="rpsQ"
                     /locus_tag="VUW49_000292"
     CDS             complement(73188..73448)
                     /gene="rpsQ"
                     /locus_tag="VUW49_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET7980269.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(73473..73679)
                     /locus_tag="VUW49_000293"
     CDS             complement(73473..73679)
                     /locus_tag="VUW49_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET7980270.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(73689..74102)
                     /gene="rplP"
                     /locus_tag="VUW49_000294"
     CDS             complement(73689..74102)
                     /gene="rplP"
                     /locus_tag="VUW49_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET7980271.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(74106..74759)
                     /gene="rpsC"
                     /locus_tag="VUW49_000295"
     CDS             complement(74106..74759)
                     /gene="rpsC"
                     /locus_tag="VUW49_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7980272.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(74772..75116)
                     /gene="rplV"
                     /locus_tag="VUW49_000296"
     CDS             complement(74772..75116)
                     /gene="rplV"
                     /locus_tag="VUW49_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7980273.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(75128..75409)
                     /gene="rpsS"
                     /locus_tag="VUW49_000297"
     CDS             complement(75128..75409)
                     /gene="rpsS"
                     /locus_tag="VUW49_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7980274.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(75513..76346)
                     /gene="rplB"
                     /locus_tag="VUW49_000298"
     CDS             complement(75513..76346)
                     /gene="rplB"
                     /locus_tag="VUW49_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7980275.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(76364..76660)
                     /locus_tag="VUW49_000299"
     CDS             complement(76364..76660)
                     /locus_tag="VUW49_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7980276.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(76660..77283)
                     /gene="rplD"
                     /locus_tag="VUW49_000300"
     CDS             complement(76660..77283)
                     /gene="rplD"
                     /locus_tag="VUW49_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7980277.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(77308..77934)
                     /gene="rplC"
                     /locus_tag="VUW49_000301"
     CDS             complement(77308..77934)
                     /gene="rplC"
                     /locus_tag="VUW49_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7980278.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(78150..78458)
                     /gene="rpsJ"
                     /locus_tag="VUW49_000302"
     CDS             complement(78150..78458)
                     /gene="rpsJ"
                     /locus_tag="VUW49_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7980279.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(78724..79341)
                     /locus_tag="VUW49_000303"
     CDS             complement(78724..79341)
                     /locus_tag="VUW49_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET7980280.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLSKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(79338..79928)
                     /gene="nrdG"
                     /locus_tag="VUW49_000304"
     CDS             complement(79338..79928)
                     /gene="nrdG"
                     /locus_tag="VUW49_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET7980281.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(79921..80427)
                     /locus_tag="VUW49_000305"
     CDS             complement(79921..80427)
                     /locus_tag="VUW49_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980282.1"
                     /translation="MELRRPRLADKETVLEMMTEFEKFQSPHDGGFWNTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVVFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(80471..80611)
                     /locus_tag="VUW49_000306"
     CDS             complement(80471..80611)
                     /locus_tag="VUW49_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980283.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(80623..82830)
                     /gene="nrdD"
                     /locus_tag="VUW49_000307"
     CDS             complement(80623..82830)
                     /gene="nrdD"
                     /locus_tag="VUW49_000307"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET7980284.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(82950..84488)
                     /locus_tag="VUW49_000308"
     CDS             complement(82950..84488)
                     /locus_tag="VUW49_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET7980285.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(84525..84668)
                     /locus_tag="VUW49_000309"
     CDS             complement(84525..84668)
                     /locus_tag="VUW49_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980286.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(84746..86278)
                     /gene="cls"
                     /locus_tag="VUW49_000310"
     CDS             complement(84746..86278)
                     /gene="cls"
                     /locus_tag="VUW49_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET7980287.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(86424..86966)
                     /locus_tag="VUW49_000311"
     CDS             complement(86424..86966)
                     /locus_tag="VUW49_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET7980288.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            complement(86963..88213)
                     /locus_tag="VUW49_000312"
     CDS             complement(86963..88213)
                     /locus_tag="VUW49_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7980289.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(88626..88931)
                     /locus_tag="VUW49_000313"
     CDS             complement(88626..88931)
                     /locus_tag="VUW49_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET7980290.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(88947..89366)
                     /gene="ruvX"
                     /locus_tag="VUW49_000314"
     CDS             complement(88947..89366)
                     /gene="ruvX"
                     /locus_tag="VUW49_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7980291.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(89370..89636)
                     /locus_tag="VUW49_000315"
     CDS             complement(89370..89636)
                     /locus_tag="VUW49_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET7980292.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(89723..90292)
                     /locus_tag="VUW49_000316"
     CDS             complement(89723..90292)
                     /locus_tag="VUW49_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7980293.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(90358..90756)
                     /gene="spx"
                     /locus_tag="VUW49_000317"
     CDS             complement(90358..90756)
                     /gene="spx"
                     /locus_tag="VUW49_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET7980294.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT        26561 a        20359 c        16657 g        27262 t
ORIGIN      
        1 cttccccatt ctatggaatc ttgcattatc cataatatag tagattgaaa ctagaatagt
       61 acacctctac ttctaaaaca ttgttagaat tcgatttgac tatcctcgat ttgtcctatt
      121 cttatttcac atcactataa aaataaggta aacttttcaa aaaagcacga aaaaagcacc
      181 cataaggtaa acttttgagt gctttgaaac attgatataa tcgtattgat taacgttttg
      241 aggcaactga ccttgtcagg ttgcagccac atttgtaagc gactaaagtc gcaacaactg
      301 tgtcaattgc accaatttag taagaaagta taaaaaaaga acaccccgaa aggtgctctg
      361 tataagtata gtaattcttt cgaattaacg tttactaaat tgtgatgctt tacgagcttt
      421 cttaagacct ggtttcttac gttcaacttt acgtgagtca cgtgtaagaa gtcctgcgcg
      481 tttcaatgaa tcgcggaagt ctgggtctac ttgaagaagg gcacgagcga taccgtgacg
      541 gatagctcct gattgaccag cgtatccacc acctacaacg ttaacgaaaa cgtcgtatga
      601 acctacagtt gaagtaactg cgaatggttg gttgatgaca agacgaaggt cagcgtgtgg
      661 gatgtactct tcaacatctt ttttgttaac agtgatttta ccagttcctg gaacaaggcg
      721 aacgcgtgca acagcgtttt tacgacgtcc agtacctgca tattgtgctt gtgacatact
      781 ttatttgttc ctttccttag ataagtcctg aaatgtcaag aacttctggt tgttgtgcag
      841 catgagtgtg ctcagctcca acaaatactt tcaacttcat accttgagcg cgtccaagtg
      901 tattgtgtgg aagcatacct ttaactgatt tctcaatcaa acgtactgca tttttagaac
      961 gaagttcacc tgcagagatt tgtttcaatc cacctgggtg gtttgagtga gtgtagtaga
     1021 ttttatcagt tgctttttta ccagtcaatt taactttttc agcattgata acaatcacga
     1081 agtcacctgt atcagtgtgt ggtgtaaatg ttggtttgtt ttttccgcga agtacgctag
     1141 caactactgc agaaagacgt ccaagtggta catcagttgc gtcaactacg taccatttac
     1201 gttcaacttg gcctggttta gccataaatg ttgttttgtt catgatttct cctatatata
     1261 gttcgatttt tgtttacggt gatgggtgtt ccttcccatc gaaaagtatt tggaaggttc
     1321 cggggccttt caaatggggt aaacaatacc gcctactatc ataccaaatt tcacccctaa
     1381 aagtcaatag atatgaaaaa tatttttcgg taacttctca aagtaactga aagttatata
     1441 atcaagttaa tttgggaatt tacctattaa cttcggtaga tttttgggaa tttttttcct
     1501 tctttttcct tgtagttaaa atccttttca aaaaatcacc ttttcttgat ttttaatcct
     1561 atttgctgta tgataaggaa aaagaaagga gacagagata tggcttttac caatacccac
     1621 atgcggtctg ctagttttgg cattgttacc agcttgcctg atgacatcat tgactctttt
     1681 tggtatatta tcgaccattt cttaaaaaat gtctttgaat tggaagaaga actcgagttt
     1741 caattgctta ataaccaagg aaagattacc ttccactttt caagtcaaca cctccctaca
     1801 gccattgatt ttgactttaa ccatcctttc gaccctcgtt atcccccaag agtactggtt
     1861 ttagacatgg acggtagaga aactatcctc ctcccagaag aaaatgacct attttaaaaa
     1921 ctctagcctt cagttgcaag taactgaaaa ctagagtttt tctatttttt caaagcatca
     1981 tacaagttgc ggatcggttg ttttaatatc ggatggataa aatgaggcgc aatttcctgt
     2041 aaggactcaa ggacaaaaag gcgttccgct atgtaaggat gaggcaatat gaggtcgtct
     2101 gtataaagga tctggtcctc cacaaagagc aagtccaaat caatcaaacg aggtccccaa
     2161 tgcacttctc tcacccgtcc cagctctgac tcaatggcta acaaggtttc taacaagtct
     2221 tgtgctggta gccaggtttc cacctcaacc acttgattgg caaagctatc ctgctccact
     2281 ccaccccaag gctccgtcgc taagacactg gactctttga gaatatggat gccacgagct
     2341 cgcagtttgt caatggcttg cttcaagttt gcttgcttat ctcccatatt gcttcctagg
     2401 gcgataaagg ctcgttgctt gcggcgatga atggttaccg agcaagtatc tagtgacaaa
     2461 tgcaccggtg cccaaggttt tttcagttcc aacttcattt cttggacaag aggataagac
     2521 tcaaaggtac gttccaccag tttataggct accgtttcaa tcaaatcttc actcgtttcc
     2581 tgaaaccaag tcgtccactg ctgacacaat tctccgtaat ggacagaggc tgttaaatcc
     2641 aagtctgtag ctgccttggt catatcatag gataggatgg ctgaaacgat aaatttctgc
     2701 cccaattctt tctcactagg aaaaagacca tgataggcaa acatttccaa atccttaatc
     2761 tgcagttgat ccataatgat tcctttctag aaaaatccgc ttgaaacgga ttctttttat
     2821 agccccatta aacgataagc ttggtcgcgg aggtccttat ctgtttcaaa tagaccacga
     2881 gctactgtcg tcaaggttgc agtgcctggt tttctaacac cacgcatact catacacata
     2941 tgttccgcct caatgataac aaaggctcct ttagcaccta gatagtccat caaggcatcg
     3001 gccacttcga tattcaaacg ttcttgaatt tgtggttttt tcgaataaac ttcaaccgta
     3061 cgggctagct tagacaagcc tgccacacga ccatctggaa tgtaggcaat gtgcgctcta
     3121 ccataaaatg gcaagaagtg gtgttcacac atggtatgga aaaagatatc cttttctacc
     3181 accatattat cgtcaataat ttcaaaggat tttgacaaat gttcctctgc tgtttgacca
     3241 agacctgaaa aaatctcttg atacatacgg gctacacgag caggtgtttc ctgcaaaccc
     3301 tcgcgattag cgtcctctcc tacagcctcg ataatcattt ttacagctgc ttcaatcttt
     3361 tgtgtatcca ttctcgttct tcctctccat cagatagacc ctcacttggc tcaagaaata
     3421 caaggaacct gtgacaatcc taactgtttg tttctcttct tttttatctg tcacattctg
     3481 ctctagaaaa tcatgccaat cttggtagct gagatttcta gacttagctg cctctttcag
     3541 cacgctttca tccgtcgccc gactatccgc aaaatgtgtt agagtaagct cggtatctgg
     3601 catggctccc agcaagtcca acatatcctc caaggctttg gttttgatac aagtgaagag
     3661 gatttcctta tgataatccg caaaacgttc ttgcaaggtt accaacaagg ccttgatagc
     3721 atggggatta tgggctccat ccaaaatcat caagggatct cttgacacga tttccaaacg
     3781 ccctggccaa cttgtttctt ccaaggcttg accaagaaag tcattgcttg ctagctctcg
     3841 accatcttct tgacaaaaag tatcaagtaa agctatggcc atcccagcat tctctatttg
     3901 gtacaaacca agcaggctag tctggaagcg accttgtctg acagcacttg tatagtcaaa
     3961 gacttcccct gtcaccacac tttcttgatg acgaacctga taatctgtcc cgtaggcaag
     4021 tctcggcgca tctttccctt ccgcaatgcg gtcaatcaca gccaaggctt ctggagcaat
     4081 actccctgtt accaagggca tcccttgttt gataatacct gccttctgct ctgctatggc
     4141 ctccaaggtg tcaccaagta aggccacatg atccaagcca atagttgtaa ttcctgtcaa
     4201 aatgggctga cagacattgg tactatcaaa aagtccaccc atgccaactt ccatgatggc
     4261 cacatctact tgctctgagg caaagtagtc ataggccagg gctgtgataa tctcaaactc
     4321 ggttgtgccc tgtaaattgg cgaccgcttc tccctccagc aaagactgat agtctgccat
     4381 gagagcttct agcctcgctt ctgagatcga ttccccattg atgctaatct ggtctgtgta
     4441 atgaatgaga tagggcgagc taaacacgcc aactctcagc cctagctttt ctagcatctt
     4501 tttcaaaaaa gcaatagtcg agcccttgcc gttagttcct ccgatatgga ggaccttgag
     4561 tttgagatgg ggattgccac gcaaagctaa cagttccacc attcgttcca agccaaaatg
     4621 cggttgatcc gtccggtagt tagcaatcca ttgattgttt tcaatttctt tcatcttatt
     4681 tatattgttt taaatctaaa ttttccgctt catcagccag acgaatggca gaggcaattt
     4741 caactgccat cctgtgacta gctacgtcat gcacgcgcac cacttctaca ccctgtcttg
     4801 cagcgatact ggttacatga gccgaagccg tgtcccgatt gcggaaacca agctctgtct
     4861 caggattgac ttcaaaacca ttctcctcta ggatattgat gacaaatcgc ttgcgcgaca
     4921 ctccgagaaa gattggatag cccttctgat gtagtttatc caggtcccgt aaaagaagca
     4981 gattttcttt cttggtcaga ccaaagccaa ttcctggatc caacaggata ttttctggtg
     5041 caataccagc ttctgccgct ctcgctagtg ctcgttcaaa gaaagcctcc atcaattctt
     5101 cgattggcaa tgtttcaaag tcagctaact cttcctctgt aaaagcttga ccaaaaccaa
     5161 aatgagggaa gatgagcgag ctagggtgct gaggtcgcgc cataactgga ttaaacatga
     5221 tgaccacttg cgctctcgct tcagctacca catgaggcat tttctcatca cccataagac
     5281 cagtgatatc attgactaga tcggcaccag cagccaaagc agcctctgct acttggctct
     5341 tccaagtatc aatagagatg aggacatcac tttccttgcg aatcgctttg atcactggaa
     5401 caacacgctg gatttcctct tctatctcaa catagctact gcccggccga gtcgattctc
     5461 cgccgatatc gagcatactg gctccttctg ctatcaattt acgagcctgc tggagtgcct
     5521 gctcaagagc aaaaaattga ccaccgtccg aaaaggagtc tggggttaca ttgataattc
     5581 cgcaaataac tgtctttgca tgattggctt tacttgacat atcggtcact ccctcaagga
     5641 ttttcatcat attatttttc tattttacca taaaaagaaa aagatggaca cgattgcatt
     5701 catctttccc tagtagaaag aggtcggttc ctacataaaa acttttacac agaaattcct
     5761 aatgtccgac tcataatcac cacaagagcc aacaaacaga aagcaatccc attaacaatc
     5821 atgtgaagca agatcgacat ttccaaacgt tgggtcttgt aggctgtcca agatagaact
     5881 gtcgacatac ctccataaat caataaagaa ggtaaattac ttggttgatg caataaagca
     5941 aacacaatcg taccgactac aaatcccaag ttctccttgc ctcggaaaat ctttttagga
     6001 ataatcccac gacacaagat ttcctcacaa atcggagcaa gcaaggctag caagaagaaa
     6061 ctggaaatca acgaactatt ttgaaccata tcattaatct gagactggtt agctgttgtc
     6121 gtctcatttg acagttgcaa caagatagaa ccaagtatat ttgacccgac aataactaga
     6181 taactcaagc ccaaacgtgc caaatcttta gctctaaaaa aagaaaaatt aaaactagct
     6241 aacttggttt tacgagctcc cataataaat agagccagaa ccacaattga aaggccagca
     6301 actatcagcc ctgactgtag cagggctact tcttttaaag tcaaaataga ggtaaccact
     6361 aggggaacct gatataaaag gacggcaagt aaaaagacca aaatccagcc tgcacgattc
     6421 aacaattctt tccacatatt tttctctttc atcacctatc tccttttgta ttaaaaaagg
     6481 ggggaaatcc ccctgggtca ttctattata aggccatgct gatgtagtta aggataaaca
     6541 aggcatccaa aatccaaatc atgacatgaa catctttaac ttgaccttta acaagcttag
     6601 tcaaagtgta agtcaagaaa ccaactgcaa tcccttgagt gatagagtag ctgaatccca
     6661 taaagataga tgtgaagaag gcaggaactg cttcagacat atcatcccaa tggatatttt
     6721 tcaagctacc aagcatcata atcccaacga taatcaagat tggagctgta gccgctgttg
     6781 gtacgatcgc tagaagtggg ctaaagaagc ttgaaatcgc aaaacagata gctacaacca
     6841 aggctgtcaa accagtacgt ccacctgcac cgataccagc agcagactca acataagtcg
     6901 ttacgtttga agtacctgca atggcaccga ctgtcgttcc aatcaaatca gagtaaagag
     6961 ccttatccaa tttggctgat tggtgatttt caccatttgt cgctacgata ccaacttttt
     7021 cacctgtacc gatcaaggta ccaattgtgt caaaaatatc tgtcaatgag aaggcaagaa
     7081 tggccatcag agtttcaggc aagcgagctg tatctgaaac caaagctccc aaaccttctg
     7141 aaccaagagc tgcaccaaag attgtcttca aatcttcaaa agctgcacca acatggttat
     7201 tagcaaaatc gatactagac aaatcaacca aaccaactgc aatagcaaga acagttgttg
     7261 tcaagataga gagaataatt ccccctttaa tccctttgat gacaaagaag atagtaatgg
     7321 caagtcctgc aagagccact aaaacagctg gattattaaa gctgaccaat cctggaactg
     7381 ctgaagagtt tgctgcaatc gttgcttgag ctttgtcagc cccttctcct acaacagtat
     7441 agttgcctgg atcaatcgtg aatttcaaaa gtccagcatt cttaatccct acataggcaa
     7501 ggaagacacc gataccagct gaaatagctg agcgaagagc attgggaatc gattcaatga
     7561 tcattttacg aacatttgtc aaggtaataa tcaatgaaat aatcccacag atgaagacca
     7621 tagctagggc ttcttgccaa gaataaccaa gcccgaatac aactgtaaag gtaaagaagg
     7681 cattgagtcc catacctggc gcttgggcat aaggtaagtt agcataaaaa gccatcatca
     7741 aggtacccgc tactgcacca ataatcgtcg ctaggaagac accctgagca ggcattcctg
     7801 tttgtgaaag tatttgtggg tttacaaaga gaatatagct cattgcaaag aaagttgtta
     7861 aaccagcgag aacctctgta cgaacgtctg taccgttctc ttttagttta aataatttgt
     7921 ccatcatcaa tctcctttta atttttacga acaataatag aattatatca tttatcattc
     7981 actttttcaa tgtctttgtt tgatttgttt aagaaattaa tccaatttca ttttttgttt
     8041 ggaatctgct tttctgcctg ctttctgtta taatagaaga catcttatct gaaaacacac
     8101 tagaaaggtc catatgacga aaaaaattat tgcagttgac ctggatggaa ccctgctcaa
     8161 ctcagacagt caaatttctg actttaccaa aaaaaccatc aaaaaagttg ctgaaaaagg
     8221 acatcaggtt attattacga caggtcgccc ttaccgtatg tcaaaagatt tttaccgtga
     8281 actgggctta gacactccta tgattaactt caacggatcc cttactcatt taccagacca
     8341 agtttgggat tttgaaaagt gtttgactgt agacaaaaaa tatctgctag atatggttca
     8401 acgttcagag gacattcaag ccgattttat cgctggagaa tatcgtaaaa aattctacat
     8461 tacaaatccc aatgaagaaa ttgccaatcc caaactattt ggtgtagaag ctttccagcc
     8521 tgaagatcaa ttccagcctg aattggtgac caagaaccct aactgtatcc tcttgcagac
     8581 cagagccagt gacaaatatg ccttggcaaa agacatgaac gccttctacc agcatcaact
     8641 ttctatcaat acctggggag gtccgctcaa tatccttgaa tgtaccccaa aaggtgtcaa
     8701 caaggccttt gctttggact acttgctcaa gataatgaat cgtgacaaaa aagatttgat
     8761 tgcctttgga gatgaacata atgataccga aatgctcgct tttgctggga agggttatgc
     8821 catgaaaaat gccaatccag agctactccc ttatgcagat gagcaaattt cccttaccaa
     8881 cgaccaagat ggggttgcca aaaccctaca agacttattc ttataaccta tactgatact
     8941 caatgagaat caaagagcaa actaggaagc tagccgtagg ttgctcaaag cactgctttg
     9001 aggttgtaga tgaagctgac gtggtttgaa gagattttcg aagagtatga ccagcttgca
     9061 agctggtctt tgtgctcttt tcacagtctc atcaaccagt taatcgattg ttctactttt
     9121 tgtctccaaa accttggaaa aggctttctt ttgtgatata cttcacatat aaatacaaga
     9181 gagctcgtca cactcgactc tgttgacaaa aaatcaatcc agtcccgttg tccctgttct
     9241 cggtcaatat caaggaggat agctatgaaa gctgttgttg taaatccaga aagcactggt
     9301 gttgctattg aagaaaaagt actccgtcca cttgaaactg gggaagcact tgtagaagtt
     9361 gaatactgtg gcgtttgcca caccgacctc cacgttgctc atggtgactt tggtcaagtc
     9421 ccaggacgtg ttcttgggca cgaaggtatc ggtatcgtta aagagattgc tccagatgtg
     9481 aaaagcctta aagtcggtga ccgcgtcagc gttgcttggt tctttgaagg atgtggcact
     9541 tgcgaatact gtacaactgg tcgcgaaacc ctttgccgta cagtgaaaaa tgctggctac
     9601 tcagtagacg gtggtatggc tgaacaatgt atcgtaactg ctgactatgc tgtcaaagtt
     9661 cctgacggac ttgatccagc ccaagcttct tctatcacat gtgctggagt aacaacctat
     9721 aaagctatca aagaagcaaa agttgaacca ggccaatggg ttgttcttta cggtgctggt
     9781 ggacttggta acctcgctgt tcaatacgct aaaaaagtat tcaatgctca tgttatcgca
     9841 gtcgatatca acaatgacaa acttgccctt gcaaaagaag taggcgctga cattgtgatt
     9901 aacggcctcg aagttgaaga tgtagctgga ctcattaaag aaaaaactga tggaggagct
     9961 cattcagcta tcgtaactgc cgtgtctaaa gttgccttca accaggctgt tgactccatt
    10021 cgtgctggtg gtcgcgtcgt cgctgttggt cttccttctg aaatgatgga actcagcatc
    10081 gttaaaacag tcctcgatgg aatccaagtc atcggttctc ttgtcggaac tcgtaaagac
    10141 ttagaagaag ccttccaatt tggtgcagaa ggtctggtag tcccagttgt tcaaaaacgt
    10201 ccagtagaag atgctgttgc cattttcgac gaaatggaaa aaggccaaat ccaaggacgt
    10261 atggtactcg acttcaccca ctaatctaat agaaacctat tttaaaagtt ggaagcttct
    10321 tccaactttt ttatattaaa ttttaaataa gaattcaaga ttactcctca aaaactcaac
    10381 atatcaacat acaaaaataa aaatttttta attttttatg ataaatagtt gcttttttta
    10441 tgaaaacggt ttatacttaa acagtcttaa agttttctta aatgaaaact aaaacaaaaa
    10501 aggaggaatg acaataatga gtatcggaat cattattgcg agccacggcg aatttgctgc
    10561 gggtattcat cagtcaggat ctatgatctt tggtgaacaa gaaaaggttc aagttgtgac
    10621 ctttatgcca aatgaaggtc ctgatgatct atacgctaag tttaataacg ctgttgctgc
    10681 atttgacgca gaagatgagg ttctagtttt ggctgacctt tggagtggtt ctccatttaa
    10741 ccaagctagt cgcgtgatgg gagaaaatcc tgagcgtaag tttgccatca tcacaggact
    10801 taacttaccg atgttgattc aagcctacac agagcgcctc atggacgctg ctgcaggtgt
    10861 agaaaaagtc gctgctaata tcattaaaga agccaaagat ggcatcaaag ctcttccaga
    10921 agagctaaat ccagttgaag aagttgcaag cgctgcagct gctccagttg cccaaactgc
    10981 tatcccagaa ggaactgtta tcggagacgg taaattgaaa atcaatcttg cccgtcttga
    11041 cacacgtcta cttcacggtc aggttgcaac tgcttggact ccagattcaa aagcaaatcg
    11101 tatcatcgtt gcttcagata acgtggctaa agacgacctt cgtaaagaat tgattaaaca
    11161 agcagctcca ggtaatgtca aggctaacgt ggttccaatt caaaaactga ttgagatttc
    11221 aaaagaccca cgttttggag aaacacatgc ccttatcttg tttgaaacac ctcaagatgc
    11281 ccttcgtgcc atcgaaggcg gcgtgccaat caagactctt aacgttggtt ctatggctca
    11341 ctcaacaggt aaaacattgg tcaataccgt tttgtctatg gacaaagaag acgttgctac
    11401 atttgaaaaa atgcgtgact tgggtgttga atttgatgtc cgtaaagtac caaatgattc
    11461 taaaaaagat ttgtttgact tgattaacaa agccaatgtc aaataagcca ttatttatga
    11521 aaggatttta aacatgtcta ttatttctat ggttttagta gtcggtgtag ccttccttgc
    11581 aggtcttgaa ggcatcctcg accagttcca atttcaccaa ccacttgtag cctgtaccct
    11641 tattgggctt gtaacaggtc acttggaagc agggattatc ctcggtggat cgcttcaaat
    11701 gattgccctt ggttggtcaa atatcggtgc tgctatcgct cctgatgctg cacttgcttc
    11761 tgtcgctgct gccattatca tggttcttgg tggtgacttt accaagacag gtatcggtgt
    11821 tgcccaagcg gttgctatcc ctcttgccgt tgctggtcta ttcttaacta tgattgtacg
    11881 tacaatttca gttggattgg ttcacactgc tgatgctgcc gctaaaaaag gtgacttcgg
    11941 cgccgttgag cgcgctcatt ttattgcgct acttttccaa ggacttcgta tcgcgcttcc
    12001 tgcaactctt ctccttatgg taccaactga aactgtacaa agtatcctta gtgccatgcc
    12061 agactggctc aaagatggta tggctatcgg tggtggtatg gtcgttgccg ttggttacgc
    12121 catggttatc aacatgatgg caactcgtga agtatggcca ttcttcgctc ttggtttcgt
    12181 tctcgctgct gtgtcagata ttactctaat cggattcggt gctatcggtg ttgctatcgc
    12241 tcttatctac cttcaccttt ctaaaactgg tggaaatggt ggcggaggag ccgcaacttc
    12301 taacgaccca atcggcgata tcctagaaga ctactaatat aagaaaggac tgaaaacatc
    12361 atgactgaaa aacttcaatt aactaaatca gatcgtaaaa aagtttggtg gcgttcaacc
    12421 ttcttacaag ggtcttggaa ctttgaacgg atgcaaaact taggctgggc ttatacactc
    12481 attccagcta tcaaaaaact ctatactaaa aaagaagatc aaatcgctgc tcttgagcgt
    12541 caccttgagt tcttcaacac tcatccatac gtagctgctc cagtcatggg ggttactctt
    12601 gcgcttgaag aagaacgtgc taacggtgtg gaaatcgatg acgctgctat ccaaggggtt
    12661 aaaatcggta tgatgggacc tcttgctggt atcggtgacc cagtattctg gtttacagta
    12721 cgcccaatcc ttggatctct cggtgcttca cttgccctta ctggcaatat cttgggtcca
    12781 ctcctcttct ttgttgcatg gaacttgatt cgtatgtcat tcttgtggta tgttcaagag
    12841 attggataca aggctggatc agaaatcact aaagatatgt ctggtggtat ccttcaagat
    12901 atcactaaag gagcttctat ccttgggatg ttcattcttg ctgtccttgt tcaacgctgg
    12961 gtaaatatta aatttgcttt cgatgtttct aaagttcaac tagatgaaaa ggcttatatc
    13021 cattgggata aattgccaga agggtctaaa ggtatccaag aagcattcgc acaagtagga
    13081 caaggattgt ctcaaactcc tgaaaaagtt actactttcc aacaaaactt ggatatgttg
    13141 attcctggat tatcaggact actccttact ttactttgca tgtacttact taagaaaaaa
    13201 gtatctccaa tcactattat ccttgccctc ttcgcagtgg gtattgtggc acatgttctt
    13261 cacatcatgt aatcaagcaa ctaaaaagga accaggttct aaaatctgat tccttttttc
    13321 tatgctttta ttcagccaag gctcccattg gatcccatgg tgcaagtacg attggttctg
    13381 ctccataggc agcttgttct tctgctgtca gcaattcctt acgaacaacg atttggtatg
    13441 tgtattcgtc catccaagcg tctgaggcaa caaagtaacc atctgtaccg accttgtctc
    13501 cccatgagtt ttcaaccttc cacttggttg atttaccatt ttcgtccaag tcaacacctg
    13561 tcaagaccat ggcgtgggtc atcaagcttt cactatagtc caaacgtcca gccttgtctt
    13621 gagtaagttt aatgtccatg cttgattcaa agtcataaac atctgtcgca aggatgccag
    13681 ccttacggtt gctgagctgg ccgacatcag aaccaaacca aacagtctca cctgcttgca
    13741 tttgggcaat cgccaattct ttcaagcgct ccattggaac gttgatgtag cgaactgcac
    13801 ggctaccaac cacattcccc aacatctcaa ctgtgtaaga ttttccgtaa ggtttatcag
    13861 cagttggagc attgataaca gaaacgtagt cttctaaagg aagattgaca tatttcttgt
    13921 aaaactcttg tggtgtgatt cctttttcac ttttgtagtt gttatcttta tcgcgataag
    13981 caaagtcaaa cttgcgtggt ggaagtccta atgacatagc aagaaagtta aagatttctt
    14041 gcaagaggtc ttctttctta gcttgaacag tcgcttgatc tgcaccagaa acaagcaagt
    14101 cacgcaagat ttgagcatct tgacgaagca atttattaag gatcgcattt agctcacgac
    14161 tgctgctaga tgaaacagac tcaggataaa ctgacttagg cacgacaccg tatttttcaa
    14221 agagggaaac gaccatatcc cattgaccgc catcttgttg aggtgtttgg agtaagaagc
    14281 taaccttgcg gctagtcaat tcttggtctg aagtcgcaat gacttgctcc aagaaccagt
    14341 ttgatttctc atacttatcc cagaagaaag tgtgggcttg tgacaactca aagttctcca
    14401 atttgtattg cgagatgagt ttgtggcgga aggtgttgag agccgcaaac atccagcaac
    14461 gaccagacgc tttctggtta gtgaccttgt ccttggttaa atccaatgag aaaacagatg
    14521 tgttgtctac atggctttgg cgacgttcca gagctgcaaa aattccgttg tggctggcag
    14581 cattttcaat cgcttggtat tttacatttg cttcatagtt ggcaaatagt ttatcagtaa
    14641 atgattcttg aatcgcgttc atagattcct ccttttattc tacagtctat tataactctt
    14701 ttcacaaagg aagcaactaa aaaacataaa aggcgaggac attgtccctc acctttttcg
    14761 aatttatact cttcgaaaat caaattcaaa ccacgtcaac gtcgccttgc cgtatatatg
    14821 ttactgactt cgtcagttct atctgcaacc tcaaagcagt gctttgagca acctgcggct
    14881 agtttcctag tttgctcttt gattttcatt gagtattaaa ctaaattaaa taatattagc
    14941 gcggagaatt tctaattctt ccttggtcaa gagacgccat tcccctcgtt ccaggttctc
    15001 atctaatact aaagttccca tagtcaatcg ttgcaagtcc accacttcct taccgcaata
    15061 ggcaaccatg cgtttgacct gatgaaactt cccttctgca atggtcacac ggatttggct
    15121 ttgattcttt tctgtatcta tggatacaag ctccagtata gcgggttgac aggtaaagtc
    15181 tttgagagga ataccctcag caaatatctc cacatcttct tgggtcatga ttcccttgac
    15241 ttgtgccaga taagtcttgt ccacatgacg cttgggcgaa agaagaacat gagccagctg
    15301 accatcattg gtcaagagca aaagaccatg cgtgtcaata tccaagcgtc ctactgggaa
    15361 aacttcctta ctccgcgcca agtcatccaa caagtccaga acggttctgt gcttgggatc
    15421 ctcagtcgct gatataactc ctttgggctt gttcatcatg tagtagacaa actcttcata
    15481 ctccaacact tgcccatcaa agcgaatctc atctattttt tcatcaatct gcaatttagc
    15541 tgatttttct tttttaccat ttacagtcac gcgcccagcc ttgagcaagt ttttgacctc
    15601 agtccgactt cccaccgcac aggcaactaa aaatttatct aatctcatag aactattata
    15661 tcatatcaaa aggaggctgg tacaatgacc aacctccttt tcgtttcata ctcttcaaaa
    15721 atctcttcaa accgcgtcaa cgtcgccttg ccgtatatat gttactgact tcgtcagttc
    15781 tatctgcaac ctcaaaacag tgtttttagc tgacttcgtc agttctatct gcaacctcaa
    15841 aacagtgctt tgagcaacct gcggctagtt tcctagtttg ctctttgatt ttcattgagt
    15901 atcagattta ggaaattaac ttcctcgtct ccaaaaaata gctaaaacaa tcatagctcc
    15961 taaaatcgct gggataatag ctgttcctga taaaactggc ccccaagttc caaagagcaa
    16021 gtgacctaga aaggctccga tccaaccgag aaacattttt ccaaaacatc ccattcgctc
    16081 tccacgattg gtcatagcac ctgctaaaaa tcccactagg agaccaacga acatacttcc
    16141 taacatatta tctccttaat ttgcccaatt cccattacgg aaaagaggta ctcgcgttcc
    16201 atcctcacga ataccatcga tatccatttg gttagaacca atcataaagt ctacgtgaac
    16261 atctgaacgg ttaagccctg cagcttcaag ctcctcttcg ctcatctccg ctccatcaac
    16321 aacgctagtc gcataggctg caccgatagc caagtggttt gacgcatttt catcgaaaag
    16381 ggtgttaaag aaggtaatgc ctgactgaga aattggactt ggatctggta ccaaggcaca
    16441 ttcacccaag gcacgcgcac ccgcattttc aaagacaagg tctttcataa cctgatcacc
    16501 cttctcagca gtgatatcta cgatttgtcc atccttaaag gtcaccttaa tgccttcaat
    16561 gatatttccg ttgtagctaa gcggttttgt agaagtgaca taaccatctg cacgacggaa
    16621 gtcaggcgct gtgaagacct cttctgttgg catatttggc aagaattctt cgccctgtgc
    16681 attgacagca ccagctgatt cccaaacgtg gttctttggc aaaccaagtg ttaaatctgt
    16741 tcctggcgct gtgtagtgaa gggctgaaaa ttgctcctta ttaagcatat cggccttgct
    16801 cttgagaatg gctgcatgtt cctcccaagc cttaacagga tctgcttcgt agacacggca
    16861 agttttgaaa atttggtccc aaaggaaatc aactgcttct tcgtcgctcg cagcatttgg
    16921 gaagactttc ttagcccact caagtcctgc tgcagctgct acagtccagc taaccttgtt
    16981 agattgagtt gcgatacgca taggcttcat ggcaagtccc atagctttag cagaagctga
    17041 aagcttgtca gcgtccactc cgttcaaggc acctggatca gatgaacgaa ctccaagacg
    17101 gctagcctta ttctccaaga gatagttcat ctcagcaatc ttgtattctg gcacattgtc
    17161 caaacgctcc atcggggcat gaaggaattt ctcacggtta atcacatcat ctgtccactg
    17221 aacgatgacc tcatgcgcac ccaaggcata agcttctttc acgattagat gtgccaattc
    17281 acgttgctcc acatcaatag agagagccaa agtgtgacca ggttgcacgt taattccgtt
    17341 cgcaaccaac aatttcgcat atttttctag attttcttta aaatttggta aaaccattct
    17401 gttttctctt ttctattttt atttttcttt caaagcagaa aagattcctg ctaaagcaat
    17461 agcacctgac aagagtgctg tcgatagaag aattgtacga tcagcaagtt ctaattgata
    17521 ttcaaatacc ttcttagccg gcttatcttc cgcaaatatt cttagtttca tgagaacccc
    17581 cttaaaatag attaaaataa atcactgtga ctgcctgtcc tcagcaaatt taaaatcaat
    17641 tccgacttgt ctactttata aaccaaaagc caatctggct gggtatggca ttcacgaact
    17701 ccttgaaaat gcttggatgc cgtcaatgaa tgatcacgat atctggcagg atgttctttt
    17761 tcttgaacca gaaaattcaa aacttcttct aatgattctg ccttcaaacc acgcttcata
    17821 gccaacttaa aatctttttt aaactgttta tgataacgaa tcttaagcac gtaagtcctc
    17881 catcaaatct gagactgatt caaaagactg gctcatatta cgattttggt ctaaatccgt
    17941 taacacttgg agcaacttcc gattttcgtc tagtctaaca tcaaaaggta atccctgata
    18001 ttgaattgcc tgacgaagga aaatattaat agctgttgtc atatccattc ccagattact
    18061 aaacacctgt tgggcctgct ccttaacctc actatccaga cggatgctta tactcatctt
    18121 tgacatactc tcaccctctt tccatagact attttaacaa aaaagaaagc taatgtaaat
    18181 ctattggata tacgttagcc tcttttaata gattattaag caatttttta aaacaactca
    18241 tcaaacaaac tcaactggtt atcctctggc atatttccaa gaatacccat ctcatccatc
    18301 ttttcaacca aggttgatga gagtccacca cgcttgcgta gttctgtttt agagaggaat
    18361 tctccctctt cacgcgcccg caccagttgc ttggcaacgt tctctcccag accatccatt
    18421 gctacaaatg gtgggataag ggtatccccg tcgatgagga actctgtcgc ctgactacag
    18481 tagagatcta atttaccaaa cttgaaacct cgttcccaca tctcattgac aatctcaaga
    18541 gttgtataga gatcgatttc cacattagag gcttcattgt tcttccgttt ttcagagatt
    18601 tcttccattc tgcgcttgat gacctccaag cccgcaccca tggtcttgat atcaaaagcc
    18661 ttagcacgaa tggagaagta agcacagtag taataaatag gatggtgaac cttgaagtaa
    18721 gctacacgca aggccatcat aacgtaggct gccgcatggg ccttagggaa catgtactta
    18781 attttcccac aggattcgat ataccactct ggcaccttat tagccttcat tgcttcgata
    18841 tagccatttc tctcctcttc tgaaatcttt agccacaaac ccttacgtac ccgttccata
    18901 atggtaaagg ccatcttagg ttccagaccc gcatgcatga ggtaaaccat gatgtcgtcc
    18961 cgacaaccga taacagtcga taggtccgct attccttgct taatcagatc ctgagcattc
    19021 cccaaccaaa cgtcagtacc gtgggacaga ccagacagct gaagcaattc cgcaaaggtt
    19081 gtcggatggg tttcgtctac cattccacgt acgaaatttg ttccaaactc tggaatcccc
    19141 aacatacccg taggcgttcc aatttgttca ggtgttaccc ctagtacatc agtcccagaa
    19201 aagagtgcca tcacgccttc gtcatccata ggaattttat tagggtcaat accagacaaa
    19261 tcctgaagtt ttcgaatcat ggtcggatca tcatgtccca gtacatcgag tttgaggacg
    19321 ttctcatcga tatcgtggaa gttaaagtga gtggtctgcc attcagccgt gacatcatct
    19381 gctggatact ggacaggcgt aaaatcgtag acatccatgt agttcggaat aacaacgatt
    19441 ccccccgggt gttggcctgt tgtccgcttg acacctgccg ctccttgagc gaggcgttct
    19501 acttctgcat cacgataaaa cttgccataa tctcgctcgt aacccttgac aaatccatag
    19561 gcagtcttgg cagctactgt accaaccgtt cccgcacgga aggcatattc ttcaccaaag
    19621 atatcacgca catccaagtg ggcgctaggc tgatcttctc ccgagaagtt caagtcaata
    19681 tcaggaacct tatccccatc aaaaccaagg aaggtctcaa acggaatatc ctgtccgttt
    19741 ttactgagtt tgtgaccaca gtttggacag tccttatggg gcatatcaaa tcctgaaccg
    19801 tacgaaccat ctgtgataaa ctcactgtac tgacactgac cacagacata gtgaggagag
    19861 agaggattga cctccgtaat cccaatcatg gtcgcaacga aactagatcc gacagaccca
    19921 cgagaaccaa ccaaataacc ccgttcatta gaacgttgca ccagcatctg cgatgccaga
    19981 taaatcacag caaatccatt ccccagtatg gatgttaatt ctttttcaat ccgcaaatca
    20041 acaatatctg gcagcggatt tccataaatc tcaaaagctt tcttataggt caactcagca
    20101 actgtttctt cagccttgtc gatgaaaggc gtatacaagt cacccttaac gacttcaacg
    20161 ggttcaaata tttctgccaa ggcattagtg ttttcaataa ccagtttacg agccagttcc
    20221 tctcccaaaa aggcaaattc atccaacatc tcattagtcg ttcgaaaatg agcctttgga
    20281 agtggtgctg gttgggcatg ttcaccatga ccgatagttc gattaatcat cgcaccctgt
    20341 cccaaactac ggacaataat ttcacgataa atctcttctt ccggttcgat atagtgaaca
    20401 tttcccgtag ccagaacagg cttgccaagg cgatctccaa cctctatcaa actcttgata
    20461 atggtctgga gttcctccat atccttgacc tgctctttgg caatcaaggg cgcatagata
    20521 gccggtggca tgacctcgat aaagtcataa tacttggcca cctcaaccgc cccatccaca
    20581 ccttgagaaa cgaccacgtc aaaaacttca ccctctgaac aggctgaacc taaaatcaag
    20641 ccctctcgat gggcatctag aaccgttctc ggaatccgtg gcactccttc aaaatacttg
    20701 gtattagaca aggaaaccag cttaaagata ttttttagac ctacctgatt cttgacatag
    20761 atggtcgcat gcttgatccg agcttttttg taagaatctg gactgattag atcaatgttg
    20821 agtctagcta aatcggtcac accatgtttt tctgctacct ctttgataaa gatgaaaagc
    20881 agacgaccag tcgcttccgc atcgtagttg gccatgtggt gatgttccaa ggccacacca
    20941 aaacgcttgg tcaaaggccc caaaccatgg cgtttatact caggatagag gtttctagca
    21001 aactccagcg tatcaataac tggctgacta atctttggaa gatcatgacg ctcataatta
    21061 gcattcataa agccaacgtc aaaggtagca ttgtgggcaa ctagaaccgt atccttgcaa
    21121 aattcttgga attcttgcaa aacttgttct agtggtttgg catttttgac atgatcatct
    21181 gtaattccag ttaactctgt agtaaaggct gacaagggat gcccaggatt gataaattca
    21241 tcaaattcag caataacatt ccccttgtac atcttagagg ccgcaacttg aatcaagtca
    21301 ttatagatag ctgaaagtcc cgtcgtttcc acgtcaaaga ccacgtaggt tgcttctgac
    21361 aagtccatct ccacttcgtt atagacgata gggacacggt cctccacgat attggcttcc
    21421 atcccataga tcagctggat tcccgctttc ttagccgcct tatagccatg tggaaaggac
    21481 tggacattcc catggtccgt gatagcaacc gccttgtgtc cccacttagc agctgttgca
    21541 acaatctctt cgacctctgg caaagcatcc atagtcgaca tgttagtatg agcatgaaac
    21601 tcaacccgac gctcaccttc tggcatcaaa tccttccgct catagtgaac aacttcctgc
    21661 acatcctgta cgttcatagt caaatcgcgt gtgaagttat tcatctccac attccctcga
    21721 actcggagcc aagaattctt cttgatgagg tcaaacttct gggcctcttc ctcgttttta
    21781 acccactttt gcatagaaaa acttgaagtg tagtccgtca ttttaaagtt gattaaaaca
    21841 cgacctgttc tagtcacttt ttgctccaca tcaaaaacaa ccccttcaaa gaccagacga
    21901 ttttcctctg tcgtcacttc gatcatagga gtaatctccg ccttatccag cttgggttta
    21961 gctgcagctt ttttcgcttg aaaatcaaag actggtttct cttccgctgg aggaggtgcc
    22021 atctgctcca gttgttccat agcacggagc gcttcctcat tggcagcttg aacaatctgc
    22081 tcattttcag catgaaaggc ctcttcctgc tcttgggtca ggacatcatt cttctcgact
    22141 tgacagttaa aagttggaaa accaaacttt tcaagttgtt tggctaaatt aggaagatga
    22201 ttcttcttaa aatgttcctt atcaatcgcc tcagatcctt caataaatag ctgattgccc
    22261 tcagcacgaa cttgcaaatt ttgataaagg gacttaaaac cttgactagc acatggacct
    22321 tcagagaaag cctccctata gtaggactgc aagagctgat ttgaaaactc ttgagaccga
    22381 gccttaattt caaaaacagc tttattgcct gtcttagaaa attcttcgct caaacctttc
    22441 tttaattcta aaaagatttc aatcggtaaa atattagaaa atacgaaatg aaactcccat
    22501 accttactaa ttttatgaac cacaactcgc tcaatattgg cctgtgctaa agcaggagcc
    22561 tgtctcattt cagcaggcat ccccaattga ttcatcaaaa tttcaaaact atttgacatt
    22621 cattttcctc acattattct tctactattt taccatattt agaggtattt tctaaagaca
    22681 aaaggaagcc actaagtgac ttccttctag agtgaggacg tattagtctt cacctttatt
    22741 tttcttaata atttcttctt gtactgactt aggtacatct tcgtagtggt caaataccat
    22801 catgaatgta ccacgtcctt gagatgcaga acgaagaact gttgcgtaac cgaacatttc
    22861 agcaagtgga acgtaagcac gaacgatttg gctgttaccg tgtgcttcca taccatctac
    22921 acgtccacga cgagcagtta cgtgacccat aacatcacca aggttttctt ctggaacagt
    22981 gattgttaca agcatcattg gttcaaggat agctggttgt gctgatttag cagcttcttt
    23041 aagggaaagt gaagccgcaa tcttgaaggc agtttcagat gagtcgacat cgtgatatga
    23101 accatcataa agcttagctt taacgtcaac cattgggtaa cctgcaagaa caccgttagc
    23161 catagattct accaaacctt tttcaaccgc tgggataaat tcacgaggaa ccacaccacc
    23221 gacgattgcg ttttcgaatt cgaatccttt accttcttcg tttggagtaa attcaatcca
    23281 cacatcaccg aattgacctt taccaccaga ctgacgtttg aagaatccac gtgcttgagt
    23341 agaagcgcgg aatgtttcac ggtaagatac ttgaggcgca cctacgttcg cttcaacttt
    23401 gaactcacga cgcatacgat caacaaggac gtcaaggtga agttcaccca tacctgagat
    23461 aactgtttca ccagtttcaa cgtttgtttc aacgcggaat gttggatctt cttcagccaa
    23521 tttttgaagg gcgataccca tcttgtcttg gtcagcttta gattttggct caaccatcaa
    23581 ttggataact ggttctggaa cgttgattga ctcaaggatg attttagatt tttcatctgt
    23641 caatgagtca ccagttgtag tatctttcaa accaacggca gcagcgatat cacctgagta
    23701 aacagtgtcg atttcttgac ggctgttagc gtgcatttga aggatacgtc cgatacgttc
    23761 acgtttacct ttagaagtat tcaatacgta tgaacctgat tgaagaacac ctgagtaaac
    23821 acggaagaat gtcaaacgac ctacgaatgg gtcagtcatg atcttgaagg caagagctgc
    23881 aaatggctct tcgtcagatg ctggacgagt ttcttcagcg tctgtatctg ggttaatacc
    23941 tttgattgct gggatgtcaa gtggacttgg aaggtagtcg ataaccgcat caagcatcaa
    24001 ttgaacacct ttgtttttga aggctgaacc acacaatact gggaagaatt caacgttgat
    24061 agtcgcttta cggataccag ctttcaattc ttcgttagtg atttcttcac cttcgaggta
    24121 tttcatcatc aattcttcgt cagtttcagc aactgcttca atcaattttt cacggtattc
    24181 ttgagcttgg tcaaggtatt cagctgggat gtcttcttca aggatatccg taccaaggtc
    24241 gttagtatag atttcagctt tcatcttgat caagtcaatg ataccacgga agtcatcttc
    24301 agaaccgatt ggcaattgga ttgggtgtgc atttgcttga agacgatcgt gaagtgtgct
    24361 tacagagtaa aggaagtcag caccgatttt gtccattttg ttggcaaata cgatacgtgg
    24421 aactccgtac tcagttgctt gacgccaaac tgtttcagtt tgaggctcaa cacctgattg
    24481 tgagtcaaga acggtaaccg caccatccaa tacacgaaga gaacgttgta cttcgattgt
    24541 gaagtccacg tgtcctggtg tgtcgatgat gtttacgcgg tggttgttcc attgagctgt
    24601 tgtcgcagca gatgtgatcg tgataccacg ttcttgctct tgctccatcc agtccatttg
    24661 tgacgcacct tcgtgagttt caccgatttt gtggatttta ccagtgtagt aaagaatacg
    24721 ctcagtagtt gttgttttac cggcatcgac gtgagccatg ataccgatat tacgagtttt
    24781 ttcaagtgaa aattcgcgtg ccatgaggtt tgtttctcct atttattttt gatttctatt
    24841 ctattataac acgattttaa taaaaacgga taggcaggac ctacccgttc tcaatgtttt
    24901 catgctattg ttggtttcag cttacgagat ggtaagctga gttatagcta atactaatcg
    24961 atttagttaa tttgaacccg ggctaaagtt agttagccga tgtgagctgg aacgagatgc
    25021 cgcgtgaaaa agataaaact ccttgtattc gtcgaatact gcgtcagttt cctattttca
    25081 ccttacatcc ttaccgttcc tagcatcatg atttagttga gctcaagcta aaagtctgga
    25141 gaaaaagatg aatctcattg gttgcaaccg caacctgctt cgattcccta ttttctctgg
    25201 gactttctta acgctttcgc atcctatctt accaacggaa gtgtgcgaat gcacggttag
    25261 cttcagccat acggtgagta tcttcacgtt tcttaactgc tgcaccagtg ttgttagcag
    25321 catccaagat ttcttttgca agacggtctt gcattgtgtg ttcaccacga agacgagcga
    25381 ttgttaccaa ccaacgaagt ccaagtgttg tacgacgttc tggacgaact tcaactggga
    25441 cttggtagtt agaaccacca acacgacgtg cacgtacttc aagtacaggc atgatgtttt
    25501 ccatagctgt ttcaaatact tcaagtgcat cgttgccagt agcttctttg atttgctcaa
    25561 aagcaccgta aacgattgaa gcagcagtac cacgtttacc atcaagcata acgcggttga
    25621 taagacgagt aactagttgt gaattgtata gcggatctgg caatacgtca cgttttggag
    25681 ctctattttt acgactcatt tctctttatc ccctttcctt atgcttttgg acgtttagta
    25741 ccgtatttag aacggccttg tttacgatcg ttaacacctg cagtatcaag tgcaccacgg
    25801 acgatatggt aacgtacccc tggaaggtct tttacacgtc caccgcgaag aagcaccacg
    25861 ctgtgctctt gcaagttgtg tccgatacct gggatgtagg cagtaacttc gataaggttg
    25921 ctcaaacgta cacgagcgaa tttacgaagg gctgagttag gttttttagg tgtcattgtt
    25981 ccaacacgag ttgcaacacc acgtttttgt ggtgaagaaa cgtttgtttg aactttttta
    26041 tgactattgt aaccaacgtt caaagctggt gatttagatt tttctacttt tgatttacgc
    26101 ggtttgcgaa ccaattggtt aattgtaggc atctacattc tcctgtgttt ttttattttt
    26161 ggtgatgata cacttggtga cagatatcat ctgtgtgtac ttttgcaaca tttgtcagca
    26221 cgtccctgta cactcttgag agaccaaaag taaaaagtac tgtctattat tgtaacaaaa
    26281 ttttcccttg cttgtcaaga tatttttctt ttttattggt aatgttagag atcaatttct
    26341 gaataaaaat agctgagaac acatcatctt cctaataaca cggcttggct tatgtgacat
    26401 ttttatctcc gtcatttata ctgatatata aaatagtacg gaaaacttct caaaagtaag
    26461 aatttctttc acaaatcaaa gaaaacgctt gcatattgtt ttactacatg ctatactgat
    26521 atagaaattt attgattgga gaatcattat gagaaaaaca ccatctcaca ctgagaaaaa
    26581 aatggtctac agcatccgtt ccctcaaaaa tggaactggt tctgtcctta tcggcgcaag
    26641 ccttgtcttg cttgccatgg ctacaccaac tatctcatcc gacgaaagta caccaaccac
    26701 taacgaaccc aacaacagaa atacaaccac ccttgcccaa cctcttactg atacagcagc
    26761 tggctctggt aagaacgaaa gtgatatttc ttcacctgga aatgcaaacg cttccctaga
    26821 gaaaacagaa gaaaaacctg ctgcaagccc agccgatcca gcaccacaaa ctggacaaga
    26881 tcgttcaagt gagccaacta cttctactag tccagtaaca actgaaacta aggcagaaga
    26941 gcccatcgaa gataactact tccgtatcca tgtcaaaaaa cttcctgaag aaaacaagga
    27001 tgctcaagga ctatggactt gggacgatgt tgaaaaacca tctgaaaact ggccaaacgg
    27061 agctttgtcc ttcaaggatg ccaagaaaga tgactacggc tattacctag atgtcaaatt
    27121 aaagggagaa caagccaaga aaattagctt cctcatcaac aatacagctg gaaaaaatct
    27181 aaccggcgat aaatctgtag aaaaactagt tccaaaaatg aacgaagctt ggttagacca
    27241 agattacaag gttttctctt acgagccaca gcctgcagga actgttcgcg tcaactacta
    27301 ccgcacagat ggcaactatg acaagaaatc tctctggtac tggggagatg tgaaaaatcc
    27361 aagtagcgct caatggcctg acggaacaga ctttacggct acaggcaaat atggccgcta
    27421 tatcgacatt cctcttaatg aagccgcaag agaatttgga tttttattac tagatgagag
    27481 caaacaagga gacgacgtga aaatccgtaa agaaaattat aagttcacag atttgaaaaa
    27541 tcatagccaa attttcctaa aagacgatga tgaatcgatt tacacaaatc catactatgt
    27601 ccatgatatc cgtatgacag gagcccaaca cgtaggcact tctagcattg aaagtagctt
    27661 ttcaacactt gtcggtgcta aaaaagaaga tatcctcaaa cactccaaca tcactaatca
    27721 cctaggaaac aaggtaacta ttaccgatgt tgcaatcgat gaagctggta agaaagtgac
    27781 ctacagcgga gatttctctg acacaaaaca tccttatact gttagctaca attccgacca
    27841 attcactacc aaaacaagct ggcgcctgaa agatgagaca tacagctatg atggcaaact
    27901 gggagctgac ctaaaagaag aaggaaaaca agttgatttg accctttggt caccaagtgc
    27961 tgataaggtt tctgttgttg tctacgacaa gaatgaccct gacaaagtag ttggaactgt
    28021 cgctctcgaa aaaggggaaa gaggaacttg gaaacaaact ctagacagca caaacaaact
    28081 cggaatcaca gatttcactg gctactatta tcaataccaa atcgagcgtc aaggtaaaac
    28141 tgttcttgca ctcgatcctt acgctaaatc tcttgctgct tggaatagcg acgatgccaa
    28201 aattgacgat gcccataaag tggctaaagc cgcctttgta gatccagcta aactcggacc
    28261 tcaagacttg acttatggta agattcacaa tttcaagact cgtgaagacg ccgttatcta
    28321 cgaagctcat gtgcgtgact tcacttcaga tcctgccatt gcaaaagact tgaccaaacc
    28381 atttgggact tttgaagcct tcattgaaaa actagactat ctcaaagact tgggtgtaac
    28441 ccatatccag ctccttccag tcttgtctta ctactttgtc aatgaattga aaaaccatga
    28501 acgcttgtct gactacgctt caagcaacag caactacaac tggggatatg accctcaaaa
    28561 ctacttctcc ttgactggta tgtactcaag cgatcctaag aatccagaaa aacgaatcgc
    28621 agaatttaaa aacctcatca acgaaatcca caaacgtggt atgggagcta tcctagatgt
    28681 cgtttataac cacacagcca aagtcgatat ctttgaagac ctagagccaa actactacca
    28741 ctttatggat gccgatggca cacctcgaac tagctttggt ggtggacgct tggggacaac
    28801 ccaccatatg accaaacggc tcctagttga ctctatcaaa tacctagttg atacctacaa
    28861 agtggatggc ttccgcttcg atatgatggg agaccatgac gccgcttcta tcgaagaagc
    28921 ttacaaggct gcacgcgccc tcaatccaaa cctcatcatg cttggtgaag gttggagaac
    28981 ctatgccggt gatgaaaaca tgcctactaa agctgctgac caagattgga tgaaacatac
    29041 cgatactgtc gctgtctttt cagatgacat ccgtaacaac ctcaaatctg gttatccaaa
    29101 cgaaggtcaa cctgccttta tcacaggtgg caagcgtgat gtcaacacca tctttaaaaa
    29161 tctcattgct cagccaacta actttgaagc tgacagccct ggagatgtca tccaatacat
    29221 cgcagcccat gataacttga ccctctttga catcattgcc cagtctatca aaaaagaccc
    29281 aagcaaggct gagaactatg ctgaaatcca ccgtcgttta cgacttggaa atctcatggt
    29341 cttgacagct caaggaactc catttatcca ctccggtcag gaatatggac gtactaaaca
    29401 attccgtgac ccagcctaca agactccagt agcagaggat aaggttccaa acaaatctca
    29461 cttgttgcgt gataaggacg gcaacccatt tgactatcct tacttcatcc atgattctta
    29521 cgattctagc gatgcagtca acaagtttga ctggactaag gctacagatg gtaaagctta
    29581 tcctgaaaat gtcaagagcc gtgactatat gaaaggtttg attgcccttc gtcaatctac
    29641 agatgccttc cgacttaaga gtcttcaaga tatcaaagac cgtgtccacc tcatcactgt
    29701 cccaggccaa aatggtgtgg aaaaagagga tgtagtgatt ggctaccaaa tcactgctcc
    29761 aaacggcgat atctacgcag tctttgtcaa tgcggatgaa aaagctcgcg aatttaattt
    29821 gggaactgcc tttgcacatc taagaaatgc ggaagttttg gcagatgaaa accaagcagg
    29881 atcagtcgga attgccaacc cgaaaggact tgaatggact gaaaaaggct tgaaattgaa
    29941 tgcccttaca gctactgttc ttcgagtctc tcaaaatgga actagccatg agtcaactgc
    30001 agaagagaaa ccagactcaa ccccttccaa gcctgaacat caaaatgaag cttctcaccc
    30061 tgcacatcaa gacccagctc cagaagctag acctgattct actaaaccag atgccaaagt
    30121 agctgatgcg gaaaataaac ctagccaagc tacagctgat tcacaagctg aacaaccagc
    30181 acaagaagca caagcatcat ctgtaaaaga agcggttcga aacgaatcgg tagaaaactc
    30241 tagcaaggaa aatatacctg caaccccaga taaacaagct gaacttccaa atacaggaat
    30301 caaaaacgaa aacaaactcc tatttgcagg aatcagcctc cttgcgctcc ttggtctcgg
    30361 tttcttacta aaaaataaaa aagagaacta aactagccct cctatagaaa aatcccccaa
    30421 gcattatagc tcgggggatt aatttttgta caatatttgt tgtcctaata aacttgatta
    30481 ggatttttta ttaagcctct ttcatagcaa aataagctcg tactttgggt gcaacttgtg
    30541 ttccgaagag ttcaatagct ctcagaacct ggtcatgagg catagaacca agcggtagat
    30601 gaagcatgaa gcggtccaat cctaaatcct ctatcatgcg aatcaatttt tcggccacct
    30661 gatctggatt gccaacaaac atggcgccat ttggccctac ctgctccaaa tattgctcat
    30721 aacgcaattc ctgccagtgc ggacggtctt tggaaatagc atccaccact tgcttagtcg
    30781 gatggaaata atctttcacc gcctgctcac catcttccgc aatccacccc caagaatggg
    30841 ctcccacttt caagtctttg tcagcatggc ccgcttcgct tccaatctca cgataagcct
    30901 gaatcaactt tttaaaataa cgtggattac caccaataat agcatataca atcggtagac
    30961 cagcctgagc aatcttcact gttgattcga catgaccacc tgtagctatc cacaagggca
    31021 atttgtcctg aactggacga ggataaactt ctttaccagc aatcgtttga gtcaatcgac
    31081 cttgccagtc taacttggtc ttttcattga ctaactgaag caagtctaat ttctcatcaa
    31141 aaagagagtc gtagtctttc aagtcataac caaacagagg gaaagattcc gtgaaagagc
    31201 cccttccagc cataatctcc gctcgtccat ttgacaaagc atcgatagtg gcatactgtt
    31261 ggaacaaacg aatcgggtcc atgcttgaca gaatgctgac tgcactggtc aaacggattt
    31321 tcttggtatt gactgcccca gcggtcagga caatctctgg ggctgatact gcaaaatccg
    31381 cccgatggtg ctcaccaatc ccatatacgt ccaaaccaac cttgtcagcc agctcaatct
    31441 ctgccaccaa ctggcgaatg cgttcagcat gactgtaagt ttgtccagtc ccttcaagct
    31501 ccgttatttc cccaaatgtt gaaattccca attctaccat tgtgattctc cttgtctatc
    31561 tctgtccttc aatttgaaaa attattctaa tactcaatga aaatcaaaga gcaaactagg
    31621 aagctagccg caggttgctc aaaacagtgt tttgaggttg tggatagaac tgacgaaatc
    31681 agtaaccata cctacggcaa ggcgacgttg acgtggtttg aagagatttt ctaagagtat
    31741 aacacgaatc ttaagtacaa gcaaccaatt tgctcattag aaaaagccta gataactaga
    31801 cttttttagc ttattctacc gttactgatt tagcaaggtt acgtggttta tccacatcga
    31861 ggccacggtg gagggttgca aagtaagcga ctaattgcgt tggtacgacc attgaaattg
    31921 gtgagaggta tggatgtacg gtcgtaagga cgatatcgtc ggtatctttg gctacattct
    31981 cttctgcgat agtgaggact ttggcaccac gggctgcgac ctcttggata tttccacgag
    32041 tatggttggc aagaactgga tctgacaaga gagccaaaac aggcgttcct tcttcaatca
    32101 aggcaatggt tccgtgcttg agttctcctg ccgcaaaacc ttcacactgg atataagaaa
    32161 tctctttgag tttgagactt gcttccatgg ctacgtagta atcttgacca cgtccgatgt
    32221 aaaaggcgtt acgagttgtt tcaagaagtt cacgaacctt ggcttcaatg gtttctttct
    32281 ctgaaagagt tgattcgata gactgagcta cgattgacaa ttcatgaacc aggtcaaagg
    32341 cttgcgcttt agcattacca tttgcttctc cgactgcttt tgcaaggaag gcaagggctg
    32401 cgatttgcgc tgtataggct ttagttgatg ccacggcaat ttcaggacct gcgtgaagga
    32461 gcatggtata gttggcttca cgtgagaggg ttgaacctgg aacgtttgtc actgttaagc
    32521 ttggaattcc catttcatta gccttgacca aaacttgacg actatccgct gtttcaccag
    32581 attggctgat aaagatgaag agtggtttct tgctgagaag tggcataccg tagccccact
    32641 cagatgagat tccaagttca actggtgtat ctgtcaattc ttccaacatt ttcttagaag
    32701 caaatcctgc atggtaagat gttccagctg caaggatgta gatgcggtct gcgtcttgaa
    32761 cagccttaat gatatctggg tctacgacaa cttgaccagc ctcatctgtg taggcttgga
    32821 tgagtttccg cataacagtt ggttgctcgt caatttcctt gagcatgtag taagggtaag
    32881 ttcccttacc gatatctgac aagtcaagtt cagcagtgta gctagcacgc tcacgacgat
    32941 ttccatcata gtcttgaact tccacactat cagccttgac gattaccaac tcttggtcat
    33001 ggatttccat gtattggtta gtttcacgaa tcatagccat ggcgtctgag cagaccatat
    33061 tgtagccttc cccaagacca atcaaaagtg gagatttgtt tttcgctaca tagatgacat
    33121 ctggattttc agagtcaatc aaggcaaagg catatgaacc acggataata tgaagagctc
    33181 ttttaaaggc ttcaagaact gagagaccgt cttcttccgc aaattttcca atcaaatgta
    33241 cggcgatttc cgtatctgtt tgccctttga agtggtgccc agcaaggtat ttttctttaa
    33301 tttcaaggta gttttcaatt accccattgt gaaccaagac aaaacgttct gtctcagatc
    33361 ggtgtgggtg agcattgtct tccgttggtt tcccatgagt tgcccaacga gtatgtccga
    33421 taccagttgt tccctcaaca ccagctgtct tggcagacaa ttctgcgata cgaccaacag
    33481 ccttcaccaa atggttatca gcaccatcta ggacaaaaat tcccgcagaa tcatagccac
    33541 ggtattcaag cttttcaagc ccttgaatca aaatatcagt tgcatttgtg tttccaacaa
    33601 caccaacaat tccacacata gtatatacga cacaggcaag ctgtgctttc tccttaaaat
    33661 tggtatagtc taattcatct tttatagaat cagcaaaaac agtatatact tgtttctttc
    33721 acttgtcaag agtaaaaatt ggtatagttc aaattaagct tctgtaagca aaaaactctg
    33781 accgattggg ataatcagtc agagtccttt ttaaaatcca ttattatcgc ttaattcttt
    33841 gaaccagtgg cctgatttct tcagacgacg ttcttgcgtt tccaagtcta attcgaccaa
    33901 accatagcga tttttatagc tgttgagcca tgaccagcag tcaataaagg tccaaatcaa
    33961 gtagccctta cagttggcac catcttcaat ggcacggtga agttcacgaa gatgaccttt
    34021 tacaaagtca atacggtaat catcttgaat cattccatct tgacggaatt tttcttcccc
    34081 ttcaacaccc ataccattct cagtcaacat ccactcaata ttgccataat tttccttgat
    34141 attttgggcg atgtcataaa tcccttgctc ataaatctcc caaccacggt gagaattgat
    34201 tttacgtcca ggcatcacat aaggctcgta aaaatgttct ggtaagagtg gactctctgg
    34261 atgcttagca aatcgaggag ccataacacg caaaggttga tagtagttca caccaaggaa
    34321 gtccaccgta ttatcacgaa tgagttccaa ctcttcctct gtagcatcag gtaaaagacc
    34381 gtgttcatgc aagatttcta ctaactcctg tggataagtt cccaagacag atggatctaa
    34441 gaaagattgg gcctgaaaaa gggccgcaat acgagctgcc ttgacatcag caggatgctg
    34501 gctacgtgga taagccggtg tcaagttgag gacaatccca atcttggaat caggcaaaag
    34561 ttcatggcaa gccttaacag cccggctgct ggccaattgt gtatgatagg ctaccttaac
    34621 agctgcctct gcatccacct tatgtggata atgggcatca taaaaataac caaattctac
    34681 aggaacgatg ggctcgttaa aggtaatcca ttgatccact aaatctccat aagtctcaaa
    34741 acaaaaacga gcatagtctt cataggctga gactgtcgcc ttattttccc aaccatcacc
    34801 atcctcttga agggcaaaag gtaaatcaaa atgatagaga ttgactaaca gacgaattcc
    34861 tttagcctta atagcctcaa agaccttacg ataaaaatcc acaccttgag ggttgacttt
    34921 tccacagcct tgtggaaaaa tccgtgacca ctgaatagaa gtccgaaagg ctgtgtgacc
    34981 agtctctaac aaaagctcaa tatcccgctc ccaattttca taaaaagtcg atgtcttatc
    35041 tgaaccaatc ccattatagt aacgatttgg ctccacttgg aaccagtaat cccagagatt
    35101 gtctccctta ccgtcaccag ctacacgtcc ttctgtttgc ggtccagaag tagaggatcc
    35161 ccagacaaaa tcctttggaa atcttagcat acatttacct ctttatctac tcatttctcc
    35221 cattatacag aaaaaacaag gtaaaaacta gttacatttt ttccttgttt ttcttctgat
    35281 tatagttttt atttcttgct taggatttca agcgtttcaa gcacgttatc tgcatgaacc
    35341 tcaatggtgt caccagttgc cttgatctta acttctacaa tgccatcggc cgctttttta
    35401 ccaacagtga tacggattgg aagaccaatc aagtcactat cgctaaattt aacaccgaca
    35461 cgttcgttac ggtcatctgt caagacttca taaccagctc ccatcaagct tgcttcaagt
    35521 ttttctgtca aggcttgcgc ttcttcatcc ttgacattga cagtaatcaa atgcacatca
    35581 aatggtgcca attctttagg gaaattgatt ccccaagcgt aacggtattc accttttggc
    35641 gttttgttaa caaagaggcg agcgtgttgc tccatcactg ctgaaagaag acggctgaca
    35701 ccgataccgt aacatcccat gatgattggc acagcacgac cattttcatc caagacatct
    35761 gctcccatgc ttgctgaata gcgagttccg agtttgaaaa tatgaccgat ctcaatacca
    35821 cgcgcaaagt taaggacacc ttgtccatct ggggaaattt caccctcacg aacttcacgg
    35881 atatccacat attctgcagt aaaatcacgg cctgggttca caccagtcaa gtggtagcca
    35941 tcttcgttag caccgacaac tgcattgcga acatcttgta ccttacgatc tgcaataatt
    36001 ttaatattct ctggcaaacc aactggtcca agtgaaccaa atcctgcttg aacaacattc
    36061 gccacttctt cttcgctagc aacgtcaaag aaatctgctc ccaagtgatt tttcaacttg
    36121 acttcgttga gttggtcatt tccaactaga agggctgcaa caagctcgcc atctgcaatg
    36181 tagaagaggg ttttaatcgt ttgttcttct ggaacattga ggaaggctgc aacttcatca
    36241 attgatttaa catctggcgt tgcaacacga gtaacttctt cttcagcgac aacacggttg
    36301 cttggtttgt actcgtttgt tgccatttct aagttagctg catagctaga ctcacttgag
    36361 taagcaatgg tatcttcacc agagactatc catttgagca attctgcctt gatttcttct
    36421 tgcacttctg caggaatttc gtcaaatgag gcaactgact tgtccaagac aacccagcgg
    36481 tcaaggtctg tacgagcaga tgtaatggcc ataaattctt ggctatcctt accacccatg
    36541 gctccaccgt caccaataat agccttgaag tctaaaccac tacgagtgaa aatacgctca
    36601 taggctgctt tgtactcatc ataaacacta tccaaactat catagttagc gtggaaacta
    36661 taagcatcct tcatgataaa ctcacgtgta cgaagaagtc cattacgtgg gcgtttttca
    36721 tcacgatact tgggctgaat ttgataaagg ttgagtggca attgcttgta agatttaaca
    36781 gaatcacgga caatagctgt aaaggtttct tcgtgagttg gacctaagat aaagtctgat
    36841 ttttcacggt tttttagttt gtaaaggtct tcaccatagg tttcgtaacg acctgattca
    36901 cgccacaatt ctgcactaag aagggctgga gccaacatct caacagcacc aatcttttcg
    36961 aattcttggc gcatgatgtt tttagctttt tcaatcacac ggttggcaag tggtagataa
    37021 gaataaacac ctgctgaaac ttggcgaaca taaccagcac gcaacataag agcatggctg
    37081 ataacttgag catcgcttgg catttcgcga agcgttggga taggcatttt actttgtttc
    37141 ataatattcc tcgattatct aaaaaagagt cgcataatgt cattccaagt cacagcaatc
    37201 atcaagacaa ccatgatgac cactccggcc aaggtgacat aggtttcaat ttcttgtttc
    37261 aatggtttgc ggcggatggc ttctaggata ttgagcacaa tcttaccacc atccaaggct
    37321 ggaatcggaa taagattaaa aatcccaata ttgatggaaa tcattgccaa gaagtacaag
    37381 atattctcaa ttccattttt agcagcatca ctacttgcct taaagatagc aacaggtcca
    37441 cccaacttgt tcaaatccgg ttggaaaatc agatttttca gagctgagag aattcggaga
    37501 gctgagtcag cagcagtggt aaaaccacct acaaacatgg atagaaaatc tgacttaacc
    37561 cccggttgaa cacctagaag gtaacgacct tgactatctt cgggtgtaac agtgacttgt
    37621 ttgtcactcc ccttttcaga aatagtcaca tccaaagtcg gtgccgtctt atctttggtt
    37681 tctgtttcca cagcttggat caagctttcc cagttgctaa cctcatgtga gccaatcttg
    37741 gtaatttgtg ccgtttctgg tactcctacc ttggccaagg caccttgggg catgatatgg
    37801 aactgattgg tatcaacatc tctgacacca ccctgcataa agattaaaac ccaaaaaaca
    37861 acgacaccta agataaaatt gttcatagga cctgcaaaat tggtaatcag tttgccccag
    37921 atagtcgcat tttgatattg aacatctaaa ggtgcaatcc gaacctcagt accatctgct
    37981 tccacaaccg ttgcatcgtg atccactgca aatgtttttt cttcttccag aaccaatcct
    38041 ttgataaaga gcttgtcttc aaaatcaaac tgggtcacct gcatagggag ggctgtttga
    38101 tccaattttt tacctgagag attgatgcgt ttaaccttac catcatcagc aagtgtcaaa
    38161 ctaacaggcg ttcctgtctt gatttcagtt gtatcatcac cccaaccggc catgcggaca
    38221 tagccaccca gaggcaagat tcgaatggta taggccgttc catccttgcc aatgtgagca
    38281 aagattttag gtcccatacc gatggcaaat tcacgtacta aaatccctga tttcttggca
    38341 aagtagaagt gcccgaactc gtgcaccact acaataatcc caaaaaccag aataaaggtt
    38401 aaaattccga gcatagcgtt tcctccgtct tttgattaaa agagtccaaa taagtgcatg
    38461 attggaaata caagcaacat actatcgaaa cgatccaaaa caccaccatg tccagggata
    38521 aatttcccag aatccttaac accaaaatga cgtttgatcg aactttctag taaatcacca
    38581 aattgtccag caatgctaaa gaaaatagca aagactgaca tcttgtaaat tccatatgga
    38641 agagcaactg tactgtcaac tatcataaag ataatggtta ctaaaattgc tcctaaaata
    38701 ccacccaagg caccctcaag ggttttatta ggcgataccc ttggtgctaa ctttcgtttc
    38761 ccatagttca tcccaacaag ataggcacca ctgtctgtcg cccagacgat acacaaggct
    38821 aagagagcct tgtccaaacc tgcaacacga gcatctagta aagcattaaa tccaaagccc
    38881 acgtagaagc tcatagcaag agggaaaacc gcatcctcaa tcgtataaga cttgctaaaa
    38941 acggtcgttc ctaacatgat tgaaatcaaa acactatagg caaccacatt cccatcaact
    39001 ggcaaaaaag tcaggtaatt ctccaaggga atggtcaatg caaaggttgc aaagagggtc
    39061 aagaggccct ccatcgtcat ggtctctaga cctctcatct tcaaaagttc atgcatggct
    39121 agcatggcta tgattccgat tgctatctga agcaagaggc ccccaatcat taaaattggt
    39181 aggaaaatag ccagggcaat ccctgcaaac aaggttcttt tctgtaaatc ctgggtcata
    39241 tttcctccta aactcctcca aatcggcgat gacgacgatt ataggcaaga atagcttcct
    39301 gcaaggccgc ttcgtcaaaa tcaggccata aagtgtccgt aaaataaagc tcactatagg
    39361 ctccctgcca tggaaggaaa ttgctcaaac gtaattctcc actagtacgg ataatcaagt
    39421 ctgggtctcg taagtcctta ggcaaatgct gagtaaagag atagttacca atcaattcct
    39481 ctgtgatgtc acctgggttg attttggcat ctaaaacatc ctgggaaatc aacttaagcg
    39541 cctgtgtaat ctcagcacgt ccaccatagt taagagcaaa attaagaatc aatcctgtgt
    39601 tgttcttagt caattcctca gccttggtta aagcttcaaa ggtttgctta ggcaggcggt
    39661 ctgtctcccc aatcatttga atcttaacat tattcgcatg tagttccggg acataattat
    39721 cataaaactc tactggcaag ttcatgataa acttgacttc ctgatctgga cgggtccagt
    39781 tttccgtaga aaaagcatag accgtaataa ccttgacgcc cagtttgttg gctgccttgg
    39841 tcacggtttg caatgcttcc atgcccgcct tgtgtccaaa aactcgcggt tgcatacgtt
    39901 ttttagccca acggccattg ccatccatga tgatgccgat atgagcagga acctgtgtcg
    39961 gaacctctac ttccacagcc ttatctttct taaaaaatcc aaacatgatc ttattcctat
    40021 tcaaaaatct atcgtttcat tataccatat ttccccattt tcttctatca ctaagctatt
    40081 tattctcagg caccaagccc atttttcaaa aaaataagcc gcctgattgg gcgactttat
    40141 ttttataggg agattattat gaaaaagttt taggagttta agttaaggtc ttcttaactt
    40201 atgaacttag tgtacactcc ctagcttaaa gtttccttaa gtatttttaa aaatcaaatt
    40261 tttccatttc tcctgccaat ttttcttgga taaacgtgtt tgatagagtt ccattcggtc
    40321 ttcattttct aagaaatgag gagttggacg aacttgaaaa ttcaaaatat cctccaaacc
    40381 ataaggtaca tagagttcaa aatctgattc ttcattcaag cgcagtccaa ctgccgtaca
    40441 ccgttctgga tacttactca tagcatcacg agaactggta taggaagcag tgtgaggact
    40501 gtgctgatgc atatagacct gatttttcaa ttcccactgg tactgaggaa aatcctctct
    40561 cagctttttc ttcagtaata aggtttcctc ataagaaaaa tctggatcaa agaaaatcac
    40621 atctatatct gtttcatgat caaaagggga tttgtctgac aaaagattcc aaatgaaatt
    40681 tctgacagaa cctgctgcca accacgagtc tttcagacca aggtctcgga tgatcgtcag
    40741 aatggccatc atatctggat tttctctaaa agcctctaag atttcttgct tatttttcac
    40801 tgtattcata acctaagtgc tcatatgcct tagcagtcgc cacccgtcca gaccgtgtcc
    40861 gcatgataaa acctttttga atcaagtaag gctcatacat gtcttcaact gtctcacgct
    40921 cttcggcgat attcacagaa agagttccta gaccaacagg tcctccactg tacatctcaa
    40981 tcatggtacg aaggattttt tgatccacat agtccaaacc ttcatggtca acatccagca
    41041 tagtcaaagc cttatcggta ataatatcat caattacccc attccccatt atctgggcaa
    41101 aatcgcgcac gcgcttgagg agacgattgg caatacgagg ggtcccacga ctacgtaggg
    41161 ccaactcaga tgctgcctca tgagtgattt ccatctcaaa aatatctgcc gtccgctcga
    41221 caatttctgt caagtcagca tgggcataat actccatatg gcctgtaatc ccaaaacgtg
    41281 cccgtagcgg attggagagc ataccagccc gagtcgtcgc accaatcaag gtaaaaggtg
    41341 gtaactccaa atgaacacta cgactaccct caccagcccc aatcataata tcgatgtaga
    41401 agtcctccat agcactataa agcacctctt ccactgacat tggcaaacga tggatctcat
    41461 caataaaaag tacatcccca ggctctaact cattcaaaat agctaccaga tctccggctt
    41521 tttcaatgac tggacccgac gtctgcttaa gattgactcc cagttcgttg gcaataacaa
    41581 aggccatggt cgttttcccg agacctggag gcccaaataa gagcacatga tccagcgctt
    41641 catcccgcat tttggcagct tcaataaaga tttgtagctg gtccttgacc ttatcctgtc
    41701 cgatatattc acgtaaatac tgaggacgga gcgtgcgttc tactaactcc tcatccccca
    41761 ttatctcatt atctaaaatt ctactcatgg ctctattata tcaaaaaacc aagccacaaa
    41821 caaaaaagcc acctgattgg gtgactccta agtttagcac ttatgtggta taatattcta
    41881 cgacactgct acaccgccta cgaaaggagg tgagatagcc catgatggaa ttagtactca
    41941 aaactatcat cggaccaatt gtggtcggtg tcgttcttcg tatagtcgat aaatggctaa
    42001 acaaggacaa atagtgtcaa aaaagacctc aagcttattt ggtcgtgagc ttggggtctt
    42061 ttctagccta tgatatagaa ctagtactca attccttttt attatcccat agttcacgaa
    42121 ttttgtcaaa ggtttacatt ttcttcaacc gctgtacgac aagacggtta agattaagag
    42181 aacgttaggg attctatcaa tttcatagaa attttgattt cgtaaacgaa gagacaatct
    42241 tacatgtcac ttctcattta atacgccact actagacaag caaaatcatt attacagtag
    42301 ttccagttct tcaattaaca gtcacttaca atcaaattga gtttgaacta gctgaagcga
    42361 ccacagacct atttcttagt tatattcgct aaaaaagtcc ccgccaattc cccgaccaaa
    42421 atccgaaaaa taccgaaaaa tatcgaaaaa ttatttttag aatagtcccc aaaagcctga
    42481 aatagagcta aaaaactcca cctgattggg tggagttaag ggagattatt atgaaaaaga
    42541 aaagtttagg attttattaa ataaagttag gaggtcttta tttaataact acatgataca
    42601 agacgaaact taaaactagc ttaacttttc taaaatttta ctattttgca aaaaatttct
    42661 atcaccagca cctcaccacg caaaaaagcc acctgattgg gtggcttcat taggagattg
    42721 tgatgaaaaa agtttaggat tttattaaat aaagttagga ggtctttatt taatgactat
    42781 ataatactag accatcctta aattttgctt aagaaaaaac aagttttgtt attttttacg
    42841 attcatccaa gtcatcccta tacaattata ggtggataag atttcaaagc ccatagaacg
    42901 atagaagccc aaggttcttt ctgtctcttc tgtcaccagc tggacttgat aggcctcttt
    42961 aaaatttcct aaagcctgtt tcatcaagga gctaccaatc ccttggcgct gatagctaga
    43021 caaaacaatc aaatcctgta caaaaactga tgaaaaacca tctccagcca aacgaatcaa
    43081 gcccacaaca gcatcaccat caagtgccag ataaattact aatgaatgat acaaggcctg
    43141 ctccagcatc tgaggttgat ttgtataatt tgtccaaccg acagcctgat agagatgcaa
    43201 aacatcctcg aacttgacga tttcttgctt tctaatagtg atcatgtcaa cacctcttaa
    43261 aactctctca agctcttgta ctgctgtcca tttttatcaa aattttcagg acgcaaccat
    43321 gtttccaaac gatctttgac tttgggccag tccttatcaa tcatagacaa ccaatccgta
    43381 tctctcgtac accccttata aaccactgcc tgacggaagg ttccttcata aataaagccc
    43441 aaacgttccg ctgctcgtct tgatagcagg ttaagagcat cgcatttcca ctcatagcga
    43501 cgatagttaa gctcttcaaa gacatagcga gccagtagat actgggcttc tgtccctatc
    43561 cgtgtccccc tgagctctgg agaaaaagtg acagctccca cttctattac tcggttattc
    43621 tgatcaattc gcatgaggga aaaagttccc aaagccttac cagttgcctt gtctatgatt
    43681 gcatagtaaa aacggtcctt acgagccaac atctgattta aaaggctgac cagttcctcc
    43741 atgtctgcta ctggctcctg aaagaggtag gtccacatct cctgaggcgt atcaggccca
    43801 taaacagcta gcaaatcctc cgcatgcttt tccactgaaa gagcctctat cctagcataa
    43861 cgcccttcta agagatcaat aaaagtcagt tcacctggtg tataaccttc cattgactca
    43921 ccaatcatct gaccatattc gtttactggt ataactttct ccttgtttcg cttttcaaac
    43981 tttatagttg cccatagtat agcgtattga aataagatga gaacaaatcg attgggaaag
    44041 taaaattaat ttctataaat gttttagcaa ttgtttcgta ctattttaga ttcagtctac
    44101 tatatatcct actttcataa gaaggttcaa aaaatcctcc agattctact ctgaaggatt
    44161 cctatcttat ttcacaacaa tgttaactaa tttagttggc ttcaatagtc cagtggacta
    44221 ttgaaggtgg cagatagagc taataaactc gttatttaac gacgatatta acgagtttat
    44281 tcggtaccgc aattactttc acgatttcct taccgtcaat ttctgctttg actttttcat
    44341 cagctaaagc gatttcttgt aattcttcac gtgatagatc tttagcaacc atgagtttgg
    44401 cacgaacttt tcctttgatt tggacgacaa tttcaatttc atcttcaacc aatttgcttt
    44461 cgtcccaagt tggccaagct acataagaga ttgactcacc tgtttctgcg actgtttgcc
    44521 agagttcttc tgccaagtga ggtgcaaatg gtgcaatcaa ttgaataaag cctttggcat
    44581 agtcaacata aagcttatct tccttgttag cagcattgac aaagaccata agttgggcaa
    44641 tagctgtgtt gaatttgaga gactcaattt gctcagtaac agctttgact gtttcgttgt
    44701 aaaccttgtc aagagcacca ttgttttccg caaggatttc tttacttgta atcaaacggt
    44761 aaactcggtc aaggaactta cggcttcctt ccaaaccttc ttctgaccaa gcaatcgaag
    44821 catcgagtgg tcccataaac atttcataaa cacgaagggt atcggcaccg tattgttcca
    44881 ccacatcgtc tgggttaaca acgttcttga gcgatttaga catcttggct ggcgcttgct
    44941 ccaactcttc ccctgtttct acatggaaga aggaaccatc acgtttttca accttgtcgg
    45001 ttgccacaag agcaccacgg tggtcacggt agcttgttcc caaaatcatc ccttggttaa
    45061 agagtttttg gaatggttcc ttagtcggaa caacaccgag gtcatagagg aatttatgcc
    45121 agaaacgagc ataaagcaag tgaagtacag catgttccgc accacccacg tagatatcta
    45181 ctggcaacca ttgtttgagg aggtcctcat cagccaattt ctcagtattg tgcgggtcaa
    45241 tatagcggag gtagtaccag cttgaaccag cccattgtgg catagtgttg gtttcacgac
    45301 gacctttgac accatcttca cgagtcactt caagccaatc tgtcaagtta gctagtggac
    45361 tttcaccagt acctgaagga cggatatcct tggttacagg caagacaagc ggcaattcag
    45421 tttcaggaac agctgttgaa gttccatctt cccaatgaat gattggaatt ggctcacccc
    45481 agtaacgttg acggctaaag agccagtcgc ggagacggta ggtaaccttc tcctgaccac
    45541 agcctttttc ttccaaccaa gccacaatct tggcaatagc gtcttctttg ttcaatccat
    45601 ctaggaagtc tgaattgaca tgcaggccat cctctgtgta ggcagcttct tcgacatttc
    45661 caccttcaag tacttcgacg attggaaggt caaattgttt ggcaaattcc cagtcacgtt
    45721 ggtcgtgggc aggcacagcc ataaccgcac ctgttccata actagcaagg acatagtctg
    45781 caatccagat tggcatttcc ttaccattga cagggttgat ggcataagca ccagtccaaa
    45841 cccctgtttt ttctttagca aggtctgtac gagccaagtc agacttaagg ctggcttggt
    45901 gtttatagtc tgctacagct tctgcttgct ctgaacttgt gatagcgtct actaattcat
    45961 gttcaggagc caagacagtg aaagtcgcac cgaaaagtgt gtccggacga gtagtaaaga
    46021 ctgtaaattc cttgtctgtt ccttttactt tgaaagttac attggcacca gttgatttac
    46081 caatccagtt gcgttgcata tccttgatag actctgacca atctagttca tctaagtcat
    46141 tgagcaagcg ctctgcgtaa gccgtgattt tgagcatcca ttggcgcatt ggtttgcgaa
    46201 caactggata gcctccacgc tcagaagttc cgtcaggaag cacttcttca ttggcaatgg
    46261 cagttcccaa ttcctcaacc cagtttactg gcacttcagc ttcataggcc aagccttttt
    46321 cgtaaagctt ggtgaaaatc cattgagtcc acttgtagta gtttggatct gttgtgttga
    46381 cttcacgatc ccagtcataa gaaaatccaa gcgcattaat ttgacgtttg aagttggcaa
    46441 tgttttccgc tgtaaattct gctgggtcat taccagtatc catagcgtat tgctctgcag
    46501 gcaaaccaaa agcatcccaa cccattgggt gaaggacatt gtagccttgc gcacgtttgt
    46561 aacgactgag gatatcggtt gcagtataac cttctgggtg tcctacgtgc agaccagctc
    46621 cagacggata agggaacata tcaagcgcat aaaacttagg ttttgatgta tctgttcctg
    46681 tcttaaatgt atgatgttct gcccagtagc cctgccactt aggctcaatt tctttatgat
    46741 tgtaaaaact catggtctct ctccaatttt gtgatgttac tattatacca tttttgaggg
    46801 aatttgaaat gtgtttaaag gaaaaactgt atactgtatc tcattaattt tgatgactat
    46861 gaattgctct atctcccact ttttatccca ttgttatgca gtaaatcaag gagtaagtca
    46921 agaagcttat ctcgtatagc acaaaatact tattttagac cgaagataaa actggaatgc
    46981 taattaatgt attcacaata aatagccgac aacctatgtc gctttttgat ttatgaagaa
    47041 catatagtag actgaatctg aaatagtacg aaacaattgc taaaacattt atagaaatta
    47101 attttacttt cccaatcgat ttgttctcat cctatttcaa tcttctatag aattcaatac
    47161 agggatcaat aaaagactac catttggaaa acagtagtcc ttattgttta ttttgaattt
    47221 acataggcat ctacagcgat aatagcttga gcaacatctg aaggcgaaat cttaaacggc
    47281 atctgatgaa ttgtctcacc ctccatagtt gcttgtttac caacttttat taaatcatca
    47341 tatccaactt gatccaaatg catttctttt agagttgttg gcataccaat ttttttgtaa
    47401 aactcaatat acttatcaag ttcttcttta ggtctatttt ccaataatag ttgtactaaa
    47461 gttccataag ctactttttc accatgtgtt aaatgatgaa tgtcacctgt caatgcagta
    47521 aaaccattat gaattgcatg cgccgcagct aatcctccac tttcaaaacc tagaccactc
    47581 aataaagtat tagcttcaac aatattttct aatgctggtg tcaccacttt agcttcacaa
    47641 gctgccatag cctgtaaacc atctgcaaac agcgtttctt cacatttctt cgcaattgca
    47701 actccagcca atgtttgctg ttgtcccaac atagtttttc catttgcctg cataaccgca
    47761 cgcgcctcaa cccaagttgc taaaccatct gcaataccag acgctaataa acgcttaggg
    47821 gcttgtgaaa taacttttgt atcaaccaaa actaaatctg gatttttaga ataaaataga
    47881 taatgatcaa atgcaccttc atctgtataa ataacagata aagcagatac aggtgcgtcg
    47941 gtcgatgcaa ttgttggagc aataataaca ggcttttcaa tcaaatctgc aatagctttt
    48001 gcgctatcaa tcgtctttcc cccaccaaga ccgataatac tatcacaatt ttctttctca
    48061 gccaaggcaa caactcgatt gatttcattg tcagaagctt caccattaaa tagcgccaga
    48121 acaatatgga aaccatacct atgtaggtaa tcttcaaatc gttttccaac aatatcataa
    48181 accaactgat cgcataatag aatagggcaa tttcccaaat ccaaaattga tttggcattt
    48241 tcaaacaagg cattttcccc ctgaatatat ctagaaggac tagcaaaaat tctcatatgt
    48301 atttcctctc tatctaaatg gaacgactat tttgaataac aaaccaatcg tcagaaaaat
    48361 catcaaccgc cttttggata gatggcatgg cgaaagctga ttcaaaaaca tccgctcctg
    48421 ctgtaacagc atgcgcacct gcagctaaag cattatttac ttgtgctaca tttttaaagg
    48481 atgcagctaa aatcttacta ggagagttct gtctatcaat agcaagagct aattgacgaa
    48541 tgacagaatt tgaatcaatg ttcagatttt ccattctatt ataatatgga gctaggtaat
    48601 ccgctcctgc ttcgatagct aataatccct gaataactgt ataaatagct gttgcagtga
    48661 tatggtagcc ctcttttttt agcgccttta ttgcacgtaa tccagctgga gtaacaggta
    48721 ctttgataaa tatatcatct cctgcttgtc ttcgaatttt atgagcatcc tttaagatgc
    48781 cttcaaaatc ttgagaaatc acctgaacat gaatagaggg tgtagagcca atcaattctc
    48841 ttacatcttt gattcgttca aaaaaattaa tagaaccctc tctttttgca atagtgggat
    48901 ttgaagttac cccagctagc ggcaaaattt cagaccactt tttaatctca tctaaattta
    48961 atgtgtcaag cataaattcc ataaaaagaa cctctttatt ttacaaagta tgctcagtac
    49021 gtcctataat gtcatcttgg gttgccttag aaagaacatt gaagaatgca gagtatcctg
    49081 caacacgaac aattaagtct ctgtgttttt caggatgttt ctgagcgtca atcagcgtct
    49141 ctctggaaac aacattgtat tgaatatggt acccatgtaa acgattaaag aatgttcgta
    49201 acaaagcaat tagttttaat ttatcttctt ctttggctaa cgtttgagga tttactttct
    49261 gatttaagag aaccccacct acgatttcat ctgttggtaa ttttgaaaca gattttaaaa
    49321 cagatgtagg gccgtgttga tccatattat gtgatggtga acaaccctct gctaacggtg
    49381 tacccgcgtt gcgtccatct ggagttgcta atgttccacg tccctgccct acgttggctg
    49441 agatagaaga tgttcctgaa taacgaattc ctccaatagg ccctcttcca taacgtgtat
    49501 taggatattt agcaatttca tcaacataaa tgtcataagc agcagtaacc aatttgtcag
    49561 cataatcatc atcattacca tacttaggtg catcatgaat caacatttct tgaatgacct
    49621 taccttcttc tccggcataa tctgtttcca gtgcatgcca aagctgactt gggcttatac
    49681 gttcttcctc aaacaccaat tttttaattg cagctaatga atccgacaaa tttgcaattc
    49741 caacttgcaa tcctgatata taatcatata ctgctccacc ttctttaagg tgttttccac
    49801 gaccaataca atcatcagtc aatgctgaac atagaatatc aggaacttct cgttccaatg
    49861 ataaatcaat agaattttca acaataacac tcattcgtgt caaatatctt agtgttttat
    49921 cccaagcatt ttctaattca gaaaagttct tcatatcctt aaaacgacca aagcttggtg
    49981 caaaccgttt acccgaagcc ggatcaattc catcattcat cgtgataagt agaaccttag
    50041 ggaagttcat ataactcata cctgtgcaac gatagcccca tttccctgga actgccgttt
    50101 caacacatcc aatggcactg taatcataag catcatcttc caatactcct tttgcaataa
    50161 aagaaggaat aataatctca tcattattaa atgcaggcat accaaaacca agtttcatca
    50221 cttcaataca ctcattcatg aaacgagcat ctaaacctgc atggtaacgt acagttagat
    50281 taggttgcgg tagatgggtt tgtgcaactg attttaatac caaataagat aatgggttaa
    50341 cagcatcctt cttatctcga gtctgtccac caattgtaac attttgatat aaaggacttc
    50401 ctgctgaaga aaatgtatgt gattgactgc gaaccttatt aattgtaatt gtcttaatcc
    50461 aaagatttgt cagacgttca acaatgctat cttctgtttc tttaccactt tctaaatcag
    50521 ccttcatata tggatacata tattgatcaa aacggccata tgaaagagag tggccattag
    50581 attcaatttg taaaatacat tgaataaacc aaactgattg aatagcttct gcaaaagtag
    50641 ttgccggttc atatgggact ctagagcaaa tatctgcaat ctcaagtaat tctttcttac
    50701 gtttaggatt tgcattttcg gctaaacttt tagcaagagc aacaaagcgc tttgcatata
    50761 ctttaatagc atcgattacg ataaatatag agtcgtaaaa atgatattta tcaatacttg
    50821 ctggatctgt taaatctaga gctacttttg ctttacgagc ccgctcttca aaacctctta
    50881 aaccaaattg caaaagtttc tgatagttaa ctgctaagtg agcatctcca gaattcatct
    50941 taccttccat accgaagaat cctgtttcca tataaacaga cacttcttca ggtaataagg
    51001 caccagctct agcacgtaaa ttattatttt cccaaaacgg agcaatactt ctaagttgtt
    51061 cttttgtttc ttctgtaata tagaaaacat ctccatcacg cttttcaaaa agatccaact
    51121 cattgagaac aaattctagc gtatattccg gaaaaatagg agcatcttta ttggaagaag
    51181 cttgatttcc cgcaatcata gattcttctt caatatagat agacatattt tccaaaattt
    51241 ctttcagcat atatgcacgt tttaggacat taggttgttc cttgtatcgt tcataggcgc
    51301 gtgttgctag aactgctctc tcagcatcaa tataaggttt tttatttaag acatcttctc
    51361 gatatttgtt catcctttca gtaaggctgc caaaatattc tgtcttgatt gttgttcttg
    51421 ctacttctgt atttaccatc gtaagcacct ttctttcgaa aatattttta tttctttcga
    51481 aaatataata ccgattttta tagaaatttt caagctttta actagaaaaa ttttaactct
    51541 ctaattttta gcacaaatgt gtgagtttaa cagtaacaca caaatacctc taagattgtt
    51601 tgatttcatt ttggcaagct aaacgatctt gtatcttaaa gaatggaaag taaaccactg
    51661 tagatatcac caatactaat aactgagtca caacaccttg ccatccaccc accaaaaatc
    51721 ctgatagaat agctggtgta ctccaaggca atgttacccc tgagaatggt tgcatgaaac
    51781 ctgttgcaat agctccatat actatcacag ctgcaagtac aggaacaaga atgaaaggta
    51841 caaacataac tggattcatg acaatcggaa atccaaatac aactggctca tttacgttaa
    51901 atattgctgg aaaagctgca acttttccta aggcttggta ttgttttgat tttgctgcaa
    51961 aaagcatggc aactacaaga ccaaacgtaa tccctgaacc tgatagaatt aaaaatgaat
    52021 ctaaaaattg ttgagtaaca atatgtgcac cattttctaa tgatagatta gcagaggcta
    52081 acatagcttt attagcatca agattagata aaagcagagc tgtcactact ccatttacta
    52141 ccgattgccc atgaacacca aaccaccaca aaaatgatat aaagaatgca attccaatag
    52201 caccatacaa agatccagtt aaaccttgca acggaacttg aatagcagaa taaatcattt
    52261 ctatgaatgt tccgccatta gtcaatgact tcgctaaaat atatacaatc atagaagata
    52321 agaaaattac aaatgctgga atcattgctt caaactgttt ggcaatagct tgtggaactt
    52381 gttctggcat cttaataaca atttttctct ttataaagaa ggtataaata cttcctacta
    52441 ccaaacctat aatgatagca ccgataattc cttggcctcc aaaccaaact ttactaatag
    52501 cgtccccaat cgcctcacct tgtttaggga tataagatga tcttagcaaa ataaagaatg
    52561 cagatacaga tagaactcca gctggtaaag cctctactcc gctattctta gcataagaat
    52621 aggcaattga aaaacaagaa attagaccca taatagcaaa agttcctgaa tatacttgca
    52681 taaacggctc tgtccaatta gctccaaaaa cactagcaat gctcttattt aatccttcga
    52741 acggcaattg tcccataatc aagaataaac taccaactac tgtcaatggc aaaattgcta
    52801 acatcccatc ttttagagct ataatgccac gcatattcac aaacttcatc atcggtgcaa
    52861 tgattttctg aacatccatc tttgacataa taaatctcct tttcttaccc actaatcaaa
    52921 gatagggcca aatctaatac ttttttccca tctaacatac catagtccat catcggaata
    52981 acagctatcg gaacatcaca cttatcacaa atttcttttg atttatctaa tgtataagca
    53041 acttgtggac ccaatagtgc aacatctata tttggcgcat aatccgctaa tttagactga
    53101 gaaaacgcct ctatttctgc ttcaactcca ctagcttgcg ctgcaatttt catattattt
    53161 acaagcatac cagtagaaaa acctgctgca caaaacaaac caatcttcac cattatgttt
    53221 tcctcctcta tgttaataac aatgataata ctctagtatt attttttatg aagttctttt
    53281 ctcaaactaa taatttcttt gattaaatta atctccgtca tactagtcat gagatgatct
    53341 tgtgaatgaa caaagagagc tgtaatttct gtctttgtac cagacgcttc ctgtgccaag
    53401 aattttgttt gtagattatg agcttctaat aaggctgcat cagctaactg gatttcttct
    53461 tcacaccgtt cacatgtacc attttttatg taatctaacg ctttataaat atgttgtttc
    53521 gcatcaccag catataaaat taaacccatg ataatttgat ctggaacaat aatttccata
    53581 aattcctcct catttcacta tgtaaaaagc ttttttcatt tgaaatttta aaattttctt
    53641 ttgtaactat agaatacaac tacataataa agttgtcaat ccaaaaacaa cagataaact
    53701 atttttgttt atataagcac aaatgtgcaa tcaactaaaa agaaaaacga taaactgaat
    53761 cagtctatca ttttcactca cctagaatct ctctaaaagt caaatcccat cttcttccaa
    53821 aacttttaaa attgtatttt tgtctgtaat caaatgattt actaaattag cacgcaagac
    53881 agaaagaatc gaagatactt tcgtatcacc gtaagcaaca gctaaacttt gaggaatatt
    53941 ttttaaatct tccaaagaga ttgctatcgt tctttcctgt aagttttcat agacttcttt
    54001 accttttgaa tcaaaaaatc gacaacaaat ttctcctaca gttttaactt tggtcaattc
    54061 tttaaagtca tcctctgtaa gcatgtctaa ccattgatgc tttcctttat tgctaaaatc
    54121 accaattccg actacagcta tatctaaatc tttccaacta tttttcaaat tttcaaaata
    54181 tcttgattgc aaaataccat ctgctaacaa tttattttct tgcacaatcg ttgcattcat
    54241 aaatgtacac tctccatgaa attttctaga catttcataa atcagtgtat tcacatggta
    54301 tttagcgtgt atgtgactag gaccacctgc tagaggatag aagtgaacat ttcggacact
    54361 tttactgtga attaaatcta ctaaattact taaacttttc ccccaagaaa agccaatttt
    54421 catattatca tcaattagat tcctaaggac acctgctgca acttgagaaa ttctttcaga
    54481 taaaattgtt ggattatcat caaattcatt tggaataatt tctaaacttt ccagaccata
    54541 tttttctttt acataatttt ccaacttaaa catattggta tcaaaattct ctatttcaat
    54601 tttaacaatt cctgcattcc ttgcttctgt taacattcta ctaatagagg ttctataaat
    54661 tcctaatttt gctgctattt gtgactgatt taagttttca atataataca gataagcaat
    54721 tttagagagc agtttattcc tatcttgatt catacattta acctcttacg aaactacctt
    54781 aaccattatc ccagcatttt ctaatgtagc tatattttgt ttagaaagtt tttcgtctgt
    54841 tattacttca tagacttgac ttaaagcaaa tcttcttact gtaccccttt tatcaaattt
    54901 acttgagtca gttagtacaa tgactttatc cgacgctgct gaaatatatt gaactacctc
    54961 actgcgcatt aaatcttttc ctgtaaagcc catctcttta tcgtaaccat ctgtcccaac
    55021 aaaagcttga tgcacatgaa aagtctgtat catttctttt aataaaggtc ccacagtaac
    55081 ctgtgaatcc ttctgaaact ctccaccaag aacaataata cgacatgaat cataagctct
    55141 cacaaaattt gctataaaaa atgaatttgt tacaatcgta acatttcttt tttgcttgca
    55201 aatttcctca gcaagtaaag cacaggtcga tccagattct atcattattg tttcattatc
    55261 tgacaccaat tttactgctt cctgaacaat ttttctctta atttcatagt taattgacaa
    55321 acgtacattt aagtcatctc cactatttaa tacagcatat ccatgctctc tgtgtaataa
    55381 accttttgac tctaatttat ctaaatcttt tctaatcgtt actttcgata catttaattt
    55441 ttccgataat gtattaacgt cgatcttttc atattctgat actaatttaa taatttgttc
    55501 caatcttttc attttacacc tccgttttat tctaccaaaa taaaaagcaa aaaacaacaa
    55561 attaaacttt cgttcgtaat tgtttttctt tcgtttttgt gataggatag acttatgaag
    55621 aggaggaact cttatggaaa tatctaaagg aattattttt aatattcaac acttttcaat
    55681 tcatgacggc ccgggtattc gtacaactgt ttttttaaaa ggatgtcctc tgcgctgtcc
    55741 atggtgttct aatcctgaat ctcaaagaat gaaacctgaa aaaatgaaag atgctcaacg
    55801 agagaaattc accttagtcg gtgaagaaaa gactgtagaa gaaattatta cagaggtatt
    55861 aaaagacaaa gaattttacg aagaatccgg tggaggttta actttatcag gaggtgaaat
    55921 atttgctcag tttgaatttg ctaaagccat cttaaaatca gctaaagaac atcacataca
    55981 cactgccatt gaaactactg cctttgttga tcatgaaaaa tttattgatt taattcaata
    56041 tgtggatttt atctacacag acctaaaaca ttataattct ataaaacata aaaaagtgac
    56101 tggggttttt aatcaaatga ttattaaaaa cattcattat gctttttctc aaaataaaac
    56161 tatcgtttta agaatcccag ttattcctaa ttttaacaat agtttagagg atgcagaaaa
    56221 attcgctact ctatttaact cattaaatat cgaccaagtt caactactcc cttttcatca
    56281 atttggtgaa aacaaatatc gtttattaaa tcggaaatat gaaatggatg gaatcaacgc
    56341 acttcatcct gaagatctta ttgattatca aaaggtattt ctgaaccacc atattaattg
    56401 ttatttctag tttatttcct tgaaatgctc tagctatttg cagataacaa gcatctatat
    56461 aaggttatca aagaagactt ccccatgact ctaaatgaat atatcttaca atatcgcctg
    56521 aaacaagcta tagataagat ggctgaatct cccaactccc ctttaagcgc tatctctgac
    56581 caagttggat tttcagacta taaatatttt gccaaagtat ttaaaaagta tctccatatt
    56641 tccccaaaga aattgaaatc actcggaaga atagtcaaat aggctattct tctttttgta
    56701 gagaatttta ctctaatata gtgaattgaa ataagatgtg aacatctcta tcaggaaagt
    56761 caaattaatt tatagaaata ttttagcagt caaagtgtac tgttatagat tcaatacact
    56821 atagaataga attgtaggtt ttaaaaacgc ttacattgtt ttaaaataaa cttaaataac
    56881 acaacggaga tatccatcat gaaaaagaaa tggatgtatt atgctgcttg ttcttctaat
    56941 gaatctgccg atgacagttc atctgataaa ggagacggtg gttcgctagt cgtttattca
    57001 ccaaactcag aaggcttaat tggagcaact attcctgcct ttgaagaaaa atatggtatc
    57061 aaaatagaac tgattcaagc tggtactgga gaacttttca aaaaactaga gtcagaaaaa
    57121 gaagttcctg tagctgatgt tatctttggt ggttcttata cacaatatgc tacccacgga
    57181 gaactctttg aaaactatat ttcaaaagaa aatgataatg ttatcaaaga atatcaaaac
    57241 acaactggct tctctactcc ttatacacta gatggtagtg ttttaatcgt ccaccctgat
    57301 ttaactaaag gcatgaacat cgaaggatat agcgaccttc tcaaacctga actaaaagga
    57361 aaaatcgcaa ctgctgaccc agcaaactct tctagcgcct ttgctcaatt aacaaatatg
    57421 ctacaagctc aaggtggtta caaagatgat aaggcttggt cttatgtaaa agatcttttc
    57481 acacttattg atggtatagt aaaatgaaat aagaaaagga caaattaatc aggacagtca
    57541 aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctagtctc aatatactat
    57601 aaaatcggtt taagttcatc tggtgtctat aaagcagtcg ctgatggaga aatgactgtt
    57661 ggtctctctt atgaagatac agcagttaaa ctcttaaatg acggagctaa cattaaggta
    57721 gtctatccaa aagaaggaac cgtcttccta cctgctagtg ctgctatcgt taaaaaatct
    57781 aaaaatatgg aaaatgccaa gaaatttatc gattttatta tctctcaaga agtacaagat
    57841 acacttggtg caaccactac taaccgtcct gttcgtaaaa atgctaaaac aagcgaaaac
    57901 atgaaaccaa ttgacaaaat caaaacactc actgaagatt atgattatgt catcaagaat
    57961 aaatcagata tcgttaagaa atacaacgaa gtctttacag atatccaatc taaacagtaa
    58021 aagaggttca ctatgagtga gatcaaaatt attaacgcca aaaaaatcta ccacgatgtc
    58081 cctgttattg agaatttgaa cattacaatt ccaaaaggaa gtctctttac ccttcttgga
    58141 gcttcaggat gtgggaaaac gacccttctt cgtatgattg caggtttcaa cagtatcgaa
    58201 ggtggagaat tttacttcga tgatacaaaa atcaataata tggaacccag caaacgcaat
    58261 atcgggatgg ttttccaaaa ctacgctatt ttcccacatt tgactgtccg agacaacgtt
    58321 gcttttggtc ttatgcaaaa gaaggttcca aaagaagaat tgattcaaca gaccaacaag
    58381 tatcttgaac tcatgcaaat tgctcaatat gcggatcgaa agcccgataa actcagtggt
    58441 ggacaacaac aacgtgtcac cttggcatgc accttagcgg ttaatccaag tgttctcctc
    58501 atggacgagc cacttagtaa tctggaggcc aaacttcgct tggatatgcg tcaagccatc
    58561 cgagcaatcc aacacgaagt gggaattaca actgtttatg taacccacga ccaagaagaa
    58621 gccatggcta tttcagacca aattgctgct atgaaagatg gggtgatcca acaaatcggc
    58681 cgaccaaaag aactctatca taaaccagct aatgagtttg tggcaacctt tatcggacgc
    58741 acaaatatta tccctactaa tcttgaaaaa cggagcgacg gcgcttatat cgtcttttca
    58801 gatggctatg cccttcgaat gccagctctt gatcaggttg aggagcaagc tattcatgta
    58861 agcattcgtc ccgaagagtt tatcaaagat gaatctggag atattgaagg aactattagc
    58921 gatagcgtct atcttggact aaatacggag tatttcattg agacaggttt tgcctcaaaa
    58981 attcaagtta gtgaagaatc aacttttgaa gaagaactac aaaaaggcaa tcgtattcgt
    59041 ctacgaatca atacgcaaaa attaaacatc ttttctgcag atggttccca aaacctgata
    59101 aaaggagtca accatggaat gtaaaaaact aaatatttgg acaacctcct ctttcttcat
    59161 ctttcttacc tatcttgtct ttctcgttta tcctatcgtt accgtgctca agcaagcact
    59221 tatacatgaa ggacaattct cactagctaa ttttgtcact ttctttagta aagtctacta
    59281 ctctgagaca ctagtcaaca gtttcaaggt ttccattacc gctactgtca cttccttagt
    59341 tgtaggaacc ctattagctt atctcttctc tatgtatgac ttcaagggga agaaatttct
    59401 acaaatattg attatcattg cttccatgtc agctcctttc gtaggagcct actcctgggt
    59461 tctcttgcta ggacgaaatg gggtcatcac taaatttttg acaaatgccc ttcatcttcc
    59521 agctatcgat atttatggat tcaaaggaat tgtacttgtc tttacactgc aactattccc
    59581 actggtattt ctatatgttg ctggaacaat gaacagtatt gacaattctc tacttgaagc
    59641 agctgaaagc atggggtcct tcggatttaa acctatcgta acggttgttt tacctctcct
    59701 agttccaacc ttactagcag ctccttgctt gtatttatga gagcattctc agactttgga
    59761 acgcctatgt tgattggcga aggatatcgg actttccctg tcctgattta tacccaattt
    59821 attagcgagg ttggaggaaa ttctgctttt gcaattatgg cgattatcat tgccttggca
    59881 attttcctta tccaaaaaca cattgcaaac cgctacagtt tcagcatgaa tctgctccat
    59941 ccaattgagc ctaaaaaaac tacaaaagga aaaatggctg ccatttatgc aacagtctac
    60001 ggaattatct ttatctctgt tttacctcaa atctacttaa tttatacctc tttcctaaaa
    60061 acatcaggta tggtatttgt taaaggttat tctccaaaca gttacaaggt agctttcaat
    60121 cgtatgggat ctgctatttt caataccatt cgtatccctt tgattgcctt agttctagtt
    60181 gttctatttg cgacatttat ctcctaccta gccgttagaa aacggaattt gtttacaaac
    60241 ttaattgaca gcctcagtat ggtaccttat attgtaccag gaaccgttct agggattgcc
    60301 ttcatttctc tggtctattt ggaagtggat ttcttatgat tacagggact gctttcatct
    60361 tgattatgtc tctatctgtc agaagattac cgtatactat tcgctcatct gttgctagct
    60421 tacaacaaat agcaccaagt attgaagaag ctgctgaaag cttaggaagt agtcgtctca
    60481 atacctttgc taagattaca actccaatga tgctatctgg tatcatttct ggagccatct
    60541 tatcttgggt cacaatgatt tcaaaactct ctacttctat cctcctctac aatgtcaaaa
    60601 caagaacaat gactgtagct atttatacag aggttctcag aggaaattac ggtgtagccg
    60661 cagccttgtc aactatcctg actgttctaa cagtaggttc cttgctcttg tttatgaaaa
    60721 tctctaaaag caatagcatt acactttagt tttccacatc aaaaacagcc gaaaggagta
    60781 ccctcggctg ttttttctta tcttgatatc cttatcaaat ccctagctca tggcaagcaa
    60841 gtctagactg attaaataga gccttcttcc atcttctcac gtcctagctc tcggtaacta
    60901 cggtcaccga caacttcaac cctaaactga ccgtcctcat attcaagaat cgtcacgcta
    60961 ccattatcta gaccatgcgg atgcatgcca ttaatcagat aaacaatggt tccaatagtc
    61021 attccatggc tgacaacaag ggcgttacct ccaccttgat cttccatttc ttttgcaatt
    61081 atttcaaagc cttccttgat tcggccactg agtttttccc agccttcagc ccaaccagct
    61141 gtatcgacct ctaccaagcc ctcagccagt tcagcataag acaattggtg aacgtggtcc
    61201 acattaaaga tacgaggaat aatgcccatg aaaagatcgc catcataggc tccatcaaaa
    61261 ctaccgaaac accattctct gatacgcttg tccatgcgat aagggatttt cccctgcaag
    61321 ccaagctctt caaggataat tcccatggtc tgaatggtac gaccagaatc actcgaataa
    61381 gcgcgctcaa actgtagatc agattctcgc aaaccgattc ctaactcttg aatccctcgt
    61441 tcaccttcag cagttaaggg agtatcgctc caaccttgcg cgcgaccaat cgtgttaaac
    61501 atggtcttgc catgacgtac caaatacaat cgtactttta ccattttccg tcctccgttt
    61561 gcttctatta tatcatgaat gataaaataa aagccaccca tacaggcgac ttttgaagga
    61621 gattattatg aaaaagttta ggagttcatt aaataaagat attagatgaa aatcaaattc
    61681 aaactaattc agtgttatct gtttcaaata atattaggag gtctttattt aatgattata
    61741 gtatacacat ctaatcttaa atagaactta aaatttctcc tattttctta atttttaaaa
    61801 ctatgaattg gtgctgggat ttgtccaccg cgagagatga agtcgacaga cgaagtccca
    61861 ttaaccttca taacgggtgc agttcctaat agaccaccaa actcaatcat atcgccttct
    61921 cttcctttgg gaatgatacg aacagctgtt gttttcatgt tgataacacc gattgctgct
    61981 tcatccgcaa tcatagccgc aatagtttca gcaggcgtat cttctgggat ggcaatcata
    62041 tccaatccaa cagaacagat agccgtcata gcttctagtt tttctaaatt aagagagcca
    62101 ttttgcactg cagcaatcat tccttcatcc tcagaaacag ggataaaggc accagataga
    62161 ccaccgacct ggttacaggc catcactcca ccctttttaa cttggtcgtt caagagggcc
    62221 aaggcagctg tcgttccatg cgtgccaact gtttctagcc ccatttcctc aaggacacgt
    62281 gccacagagt ctccaaccgc aggggttggt gccaaactca agtccacaat accaaactcc
    62341 acacccagtc tctcactggc catttgacca accaattgac cgatacgagt gattttaaag
    62401 gcagttttct taactgtttc ggctactaca tcaaagctct gtccacgaac tttttccaaa
    62461 gcacgtttca caacaccagg accagaaact ccgacattga tgataacatc tgcttcccca
    62521 acaccatgaa aggcacccgc cataaatgga ttgtcctcaa cagcattagc gaatacaacc
    62581 aacttggcca ctcccatatc tgaaagattt gctgtttcct tgataattcg tcccatatct
    62641 gccacagccg tcatattaat accagacttg gttgagccga tattgactga cgagcagacc
    62701 ttatccgtct cagccaaagc gcgaggaatg gaattgatga gaatctcatc tcccttttga
    62761 taaccttttt gtactaaggc agaaaaacca ccaataaagt ccacaccaat ctctttcgca
    62821 gccttatcaa gcgcttttgc cagaaccacg tagtccgtcg catctgtcgc tgccccaatc
    62881 agagaaatag gtgtcaccga tacacgctta ttaacgatag gaattcccaa ctcagccgca
    62941 atttcatcac caacagctac taaattagcc gcctttgtcg taattttttg atagattttc
    63001 tccgcagcac gattgatatc tggatcgata cagtccaaaa gagaaatccc catggtaatg
    63061 gttctgatat cgaaattttg ctcctcaatc atggcgatgg tttcagtaac ttgtctaata
    63121 tccatgataa cctcctagat attatacata gcttcgaaaa tcgctgcact ctgaatattg
    63181 atttttacat tcaaagtttg cccaaaagct tcaaattcat tacgaagata ggtaaaatct
    63241 tgcttttcat cactagatac aacagccatc atcgtaaaat attcatccaa tacagtttga
    63301 gagatatcgt caatattcaa tcctaattct gcaattttac cagaaacacc tgcaacaatt
    63361 ccagatttat ctttaccaac aacagttata atcgctttca taagtaaact ccaattcttc
    63421 ttaaaataag aaaccttaac attaaacagg attcttttca aatagtatag cataattcta
    63481 acgaaaatac tcaaaaacgc ctgcttacga agttgttctt aagaaaaaaa caatttcgta
    63541 aacaagcgtc tactatccca acttatgatg agtgttcccg ctaggaccga aatcctagcg
    63601 gtagaccaga gctagactaa gagcacaaga ctccatcatc ataacactca acaaaattga
    63661 tgattttata ctaattcgat gatcgccatt ggcgctgcat caccacgacg tggttcagtt
    63721 ttaaggatac gagtgtatcc accgttacgt tcagcataac gaggtgcgat ttctgagaac
    63781 aatttttgaa gtgctgtagt agaagtgtac ttatcagttg cttcatcata gttttcagat
    63841 gcgatttcat tacgtacgaa agcagctgct tgacgacgtg catgcaaatc accacgttta
    63901 cctagagtaa tcattttttc aacagtttta cggatttctt tagcacgagc ttcagttgtc
    63961 acgattgatt cgttgatcaa aaggtcagtt gtcaaatcgc gaagcattgc tttacgttgt
    64021 gagctagtgc gtcctagttt acggtaagcc atgtattcct cctttattta tcttttaatc
    64081 caagacccaa atcaatgagt ttgagtttca cttcttccaa actcttgcgt ccaagatttc
    64141 gtactttcat catctctgct tcagattttt ctgtcaaatc atgcacagta ttgataccgg
    64201 cacgttttaa acagttgtat gaacgcacag acaagtccag ttcctcaatc gtacgatcta
    64261 aaatacggtc gtcagattca gtatcagctt ctttcatcac ttcagttgac ttagcaatct
    64321 cagtaagatt tgtaaacaaa tcaagatgtt ctgtcaaaat acgtgctgaa agccctaaag
    64381 catcttctgg aataattgtt ccatttgtca agatttcaag ggttaatttg tcaaaaccat
    64441 cattgctacc tacacgagca ggttccactt gatagttgac ttttgtaact ggtgtataaa
    64501 tagaatctac agcaagtgtt ccaactggtg cattatcctt tttattttca tcagcaggta
    64561 catatccacg accactgtta acagtcatag tcgcttttag agaagaacct tcaccgattg
    64621 taaagagata atgatctgga tttacaattt caatatcgct atctgtcaaa atgtcaccag
    64681 ctgttacttc agcaggacct tcaacatcca gttcgatgat tttttcgtct tcaacgtacg
    64741 atttcactgc aattccttta atgttcagaa tgatttgcat cacgtcttca cgaacacctg
    64801 gaactgtgtc aaactcatgt aacacaccat caatgttgat agatgtcaca gctgctcctg
    64861 gtagagaagc tagaagtaca cgacgaagag agttaccaag agttgtaccg tagccacgtt
    64921 caagtggttc gattacaaac ttgccataat ctttattttc atcaattttt gttatatttg
    64981 gtttttcaaa ctcgatcatt tagttactcc ctcttaaacg aaaagcagtg taatgcgatg
    65041 attatacacg gcgacgtttt ggaggacgag caccattgtg tggcactgga gtcacatcac
    65101 gaattgctgt tacttcaaga ccagcggcag caagcgcacg aatagctgac tcacgaccag
    65161 aacctggacc ttttacagta acttcaactg atttaagacc gtgttcttgt gcagatttag
    65221 cagcagcttc agaagccatt tgagcagcga atggtgtaga tttacgagaa cctttgaaac
    65281 caagagcacc agctgatgac caagcaattg cattaccatg cacatcagta atcataacaa
    65341 tagtgttatt aaatgtagcg tgaatatgag caataccaga ttcgatattc tttttcacac
    65401 gacgtttacg tgttggttta gccaagactt ttacctccta tattattttt tcttaccagc
    65461 aatcgcaaca gctttacctt tacgagtgcg agcgttgttt ttagtgtttt gtccacggac
    65521 aggaagtcca cgacggtgac ggataccacg gtatgaaccg atttccatca aacgtttgat
    65581 gttcaagttt acttcacgac gaaggtcacc ttcaactttg attacatcca cttcacgacg
    65641 gatagcatct tcttgatctg atgtaagatc acgtacacga acatcttctg agattccagc
    65701 agcagccaaa attttcttag atgttgcaag tccgatacca taaacataag tcaatgagat
    65761 tactacgcgt ttgtcatttg gaatatcaac tccagcaata cgagccatgt tttctccttt
    65821 ctatcttatc cttgacgttg tttgtgtttt ggatttgctg ggcaaattac cataacacga
    65881 ccattacgac gaataacttt acagtattcg caaattggtt tgaccgatgg tcttactttc
    65941 atttcttatc cctccaagtt tttcgattat ttaaagcggt aagtgatacg tccacgtgtc
    66001 aagtcatatg gactcatttc gacagtaaca cgatctcccg ctaaaatacg aatatagttt
    66061 ttacgaattt taccagaaac tgttgctaaa atctgatgtc cattttcaag ttcaaccgta
    66121 aacattgcat tcggcattgt atcaactact ttgccttcaa cttcaatcac atcgtctttt
    66181 gccacgcaaa agcacctcca taaatttcga ttcgatgcct ctagacacag agacaacaat
    66241 tataagtcag actatctcag tataacattt gtagcggatt ttggcaagtg tgaaaaacgc
    66301 tttatttcaa atttgtcaat actttttcaa tatctgagaa gacatcattg atatcttgat
    66361 taccttcgat gtcatgaacc aaacctttgg cacggtagtg agcaatgatt ggttctcctt
    66421 gagcaatatt aacatccaaa cgacgtttta ctgtctcagg cttatcatct tcacgttggt
    66481 agtaatcttc ttctttatag tcaactggtg ggttaaagac cttgtggaaa gtttctccag
    66541 ttacgcggtg gatgatacgg ccactcaaac gttccaaaag gctgttaggg ttcacttcaa
    66601 tattgataac accttctagt tcaatgccaa gttcagccaa tgttttgtcc aaggcctgag
    66661 cttgttcaat tgtacgtggg taaccatcca ataagaatcc tgtttcttta atatcatctt
    66721 gtgaaagacg ttcttttacg attccatttg taacttcgtc aggaaccaat tcacccttgt
    66781 caatatatga cttagcaaga acacccattt cagtttgatt tgccattgca gcgcggaaca
    66841 tatcacctgt tgagatatgt gcaacatgga attgttctac gatttttgct gcttgagttc
    66901 ccttacctgc accaggtaag cccataatca aaagattcat gatttgatct ccttatttta
    66961 tttttaaata gaagcaaaaa gtcttttcac ccctatttaa aaacaaaata gaagagggga
    67021 gatccaactc ttacaaatgt cagagtttaa acctccactc cctcagtatt tactgattca
    67081 gtaaatactt ttattctgtt ctgtccatga aaccaacata cttacgtttc aataggtaac
    67141 cttccaattg cttgattcct tcgatacctg tagagataat gatcaagaga cttgttccac
    67201 caaaggcaac aacatcagaa agaccaaata catctttagc tgcaatcggt aaaatggaaa
    67261 tcacaccaag gaagagggaa ccaacagttg caagacgacg aagaagttta gacatatatt
    67321 cttctgtacc tttaccagga cgaactccat ggatataggc accactcttt tgtaggctct
    67381 ctgctgcttt ttcaggatta atctgtacaa acgtatagaa gaatgtaaag agaataatca
    67441 acaaagcata catggcaata ccagttggag aagtagttgc caacatctct tgtgctaccc
    67501 ttacccaagc ccaatcatga cctgtggcac tcaaaaactg aagaatagcc gcaggcgctg
    67561 cagtaatcga actggcaaag ataacaggga taactccagc aggatttacc tttaacggaa
    67621 ggtaagagct agatggagca ccttgtgcaa ccttagtata ttggattgga attttgtatt
    67681 ctgcttgttg aacataagtt gtaaagtaaa taatcaacaa tacagtaata atcaaaatga
    67741 ttacgaaaat gatagatgaa gtgatacggc tacttgggac gttcacaaag tagtccacat
    67801 agatgccctg aatcatctct ggaattgagg aaacaatccc ggcaaagata atcatggaaa
    67861 caccgtttcc gtatccctta tctgtaattt gctcacccaa ccaagtgaca atcatactac
    67921 cagctgttaa gatgatacca atcgtcagaa aaacttgtgg agttaaagca gttttaatca
    67981 attgagctcc agccaaggta ttaaaaccag ctgtaatccc gatagattgc acaaaagcga
    68041 gaactagagc aatataacga gtagcttgat tcaattttct tcgacctact tccccttgtt
    68101 taccccactc tacaaacttg ggtaaaatat ccatttgcaa gagttggaca acaatagaag
    68161 cagtgatata gggactaact cctagggcaa aaatcgaaaa gttttttagg gcattccccg
    68221 acaccaagct caacatgttt aagaaggata atccacttaa agcattcaag ctattggcat
    68281 tcacaccagg aactgtaatg ctagttccga tacgaaagac caaaacgata aaaattgtaa
    68341 ataaaatttt tgatcgaacc tgcttgactt taagagcttc tcttaataat ttaaaaaaca
    68401 taggtcacct ctcttagatg acttctactg aaccaccttt agcagtgata gcttcttcag
    68461 ctgatttaga gaatttagct gctttcacag tcaatttctt agtcaactca ccgttaccaa
    68521 gaattttaat acctgacttt tcagctttaa caattcctgc ttcgataaga acaactggag
    68581 ttacttcagc accatcttca aagacgttca attggtcaag gttcacaatt gcgtattctt
    68641 tagcgttgat gttagtgaat ccacgttttg gaagacgacg gaacaatgga gtttgtccac
    68701 cttcaaaacc aaggcgaact ccgccaccgc tacgagcttt ttgacctttt tgaccacgac
    68761 cagatgtttt accgttacct gatgaagtac cacgaccaac gcggttacgt actttacgag
    68821 aaccttctgc aggtttcaat tcatgaagtt tcatttttat tttctcctct tttgtaaaat
    68881 gctagcgccg ataagggaga aaaggttgtc tcccccatca actcgcctat acgatgtcat
    68941 catagatgac tatatctagt tttaggggat ggtacagtgc acatccccta aaacttcatt
    69001 agtttacttc ttcaactgtt actaagtgag atactgctgt gatcatacca cggatagcag
    69061 cgttatcttc tttaataaca aagctgttca atttgccaag tccaagtgct acaacagttt
    69121 tacgttgtga tggaatgcgt ccgattggag acttagtcaa agtaatttta atttgagcca
    69181 ttttatcccc tttcttatgc caaatcagaa actgaaatac cacgaagggc agcaatttct
    69241 tcagcgcgtt tcaattgttt caaaccttca acagttgcac gaacaatgtt gattggagtg
    69301 ttagaaccaa gtgatttaga tgtaatatct gccacacctg ccaattccac aacggcacga
    69361 actgcaccac cagcggcaac tccagaacct tctacagcag gtttcaacaa tactttagct
    69421 ccaccgaatt ctgaaagaac ttcgtgtggg attgttgttc caaccatagg aacttcgatc
    69481 aagtttttct tagcatcatc tactgcttta cggattgctt ctggaacttc ttgagcttta
    69541 ccagtaccaa atcctacgcg accattgtgg tcaccaacaa caacaagagc tgcgaaacga
    69601 agacgacgtc cacctttaac aacttttgta acacggttga cagcaactac gcgttcttct
    69661 aattcaactg cattgtcttt aaatgccatt ttctagtgtc ctcctattag aatttcaatc
    69721 cgttttcacg agctgcatca gccaaagctt tcacacgtcc gtgatataga tatccaccgc
    69781 ggtcgaacac cacttctgaa atacctttag cgtttgcacg ttctgcaacg agtttaccga
    69841 cagcaacggc ttgttcagtt ttagttcctt ttgaaacttc tttatcaaga gttgaagcac
    69901 ttgcgagcgt tacacccgct acgtcatcaa tcacttgagc gtagatgcct gtattagaac
    69961 ggaatacgtt caaacgtggg cgatcagcag ttccagagag ttttccgcga acgcgacggt
    70021 ggcgtttttg gcggagtttg tttttatctg gttttgaaat cacagttttc acctctttag
    70081 ttttaaatcg tgtgctatgc acaaagttgg aaaataggtt ggtggttgat gatcaaccac
    70141 tcaacattat ttacctgttt taccttcttt acggcgaacg aattcaccaa cgtaacggat
    70201 acctttacct ttatatggtt ctggtgaacg aaggctacgt acgtaagcag ctgtttgacc
    70261 aactacttct tttgaaattc cgctaacaac gattgttgtt gggtttggaa gttcaaaagt
    70321 aattccttct ggagcttcaa cttcgtctgg atgagattta ccaacagcca aaacaagttt
    70381 agatccttga agctgtgcac ggtaaccaac cccacgcatt tcaagttctt tcttgaatcc
    70441 ttctgataca ccaacaacca tgttgttcaa aagggcacga gtagttccgt ggatagtttt
    70501 catttctttt gaatcgtttg gacggtgaag agttacttca gtaccttcca cacggatttc
    70561 aatatctttt gagaactcac gagtaagttc tcctttaggt ccttttacag ttacaacgtt
    70621 gtcattgtta gtgagttcaa caccagcagg caacacgata actttattac caatacgtga
    70681 catgtttatt ttctcctgtt aaattgtcag gccagaacgg ccagttttca cggggttcag
    70741 atacttattt agttcaagag ctgaactgaa cacgctctaa gaactgtgca aaatagataa
    70801 gttggcttgt ttgtaaacaa acttctccaa cttcctaatt ttgctttgtt ctttacgagc
    70861 tttgtatctt gattttacca aacgtaagcg ataacctcac caccaacatt cttttggcgt
    70921 gcttctttat cagtaagcaa accttcagaa gttgaaagga tggcaattcc aagtccgtta
    70981 agaacttttg gaaggtcttc acgttttttg tagacacgaa gtcctggttt agaaacacgt
    71041 ttcaagttag tgataacttt ttcaccattt ggtccgtatt taagaaatac acggatgacg
    71101 ccttgtttgt catcttcgat gatttcaacg ttttttacaa aaccttcgcg tttaaggatt
    71161 tcagcaatcc cttttttgat gtttgatgca ggtacttcaa gtacttcgtg tttagcttgg
    71221 ttagcattac gaatacgagt taggaagtct gcgattgggt cagtcataac cattttgttt
    71281 ttctcctctt actagtagtt tgcaagttgc acttgctagt taatacatga tacaaagagc
    71341 gtaacagcac gagcaaaaat agacaattta atgcagaagc aatgctccaa agcaaattgg
    71401 tcttttttgc caaagctgta gctcgggttc aaattggcaa gcccaattga acccgggcta
    71461 aactctgtgt gaaaaagata aactttccta gaaactttag tttcttcgtc aagtttccta
    71521 tttttacttg gagttttgac acccttgata tcttaaatta ccaagatgct tttgttacac
    71581 caggaatttg acctttatgc gcaagttcgc gaaaagcgat acgactcaga ccaaatttgc
    71641 ggtaaactga atgtgggcgc cccgtaactc tacaacgatt atgtaaacga gtcggtgagg
    71701 cgttgcgagg taatttagat aaaccttcgt agtcccctgc cgcctttaat gcagcacgtt
    71761 tttcagcata acggtcaaca attttttggc gtttagcctc tctagctacc attgattttt
    71821 tagccattag atttacctcc tatattattt tgcaaaaggc attccaaggc ctgtaagcaa
    71881 tgcacgtgac tcttcgtcag tgttagcagt tgttacgata acgatgtcaa gaccacgagt
    71941 tttgtcaacg tcatcgaagt tgatttctgg gaagattaat tgttctttca caccaagtgt
    72001 gtagttcccg cgtccatcaa atgattttgt tggaacaccg tgaaagtcac gtacacgtgg
    72061 aagtgaaact gataccaatt tatccaagaa ttcgtacata cgttcaccac gaagggtaac
    72121 ttttgcaccg atcgcaacac cttcacgaag acggaagccg gcgattgatt ttttagcttt
    72181 agtgataagt ggtttttgac ctgagataag tgccaattct tcagcagctt tttcaaggct
    72241 tttagcgttt gatacagctt caccaacacc catgttcaaa acaatcttat ctactttagg
    72301 cacagccatc actgatgagt agttgaattg ttctgtcaaa gcaggaacta cttcattaag
    72361 atatttttct tttaaacgat ttgccattat acttctcctt tccttcgtga ttaatcaagc
    72421 acttcgcctg attttttgtt gtagcgaact tttttaccgt ctacaaattt gtaaccaaca
    72481 cgaccagcta caccattttt gtccaaaact tgaacgtttg atacgtggat agctgcttct
    72541 ttctcgatga taccaccttg aggaagctcg ttagttggac gttggtgttt cttaacaatg
    72601 ttaacacctt caacgataac tttgtttact tttggaaggg cagtaaggac aacagcttct
    72661 gttcccttat ctttaccagc gattacgcga actttgtcgc ctttttttac aaacattagg
    72721 tttctccttg atttttctta cgcccataag ggcaccctag cttgaagcta ggggactagt
    72781 ttgttcctaa aaattaaagt acttctggag caagtgacac gatcttcatg aagccacctt
    72841 cacgcaattc acgtgcaact gggccaaaga tacgtgttcc gcgaggagtt ttgtcttcac
    72901 ggatgataac tgctgcgttt tcgtcaaatt tgatgtatga accatcagca cgacgagcac
    72961 ctgatttagt acgaacgata actgctttaa caacgtcacc ttttttaacc gcaccaccag
    73021 gagtagcttg ttttacagat gccacgataa catcaccgat gtttgcaaat ttacgtcctg
    73081 aaccaccaag aactttgata gtcaagattt cgcgagcacc gctgttgtct gcgactttca
    73141 aacgagtttc tgtttgaatc atttcagttt tctcctttca ggtttgatta gatgatgacc
    73201 gcttcttcaa caacttctac aagacggaaa cgttttgtag ctgaaagcgg gcgagtttcc
    73261 atgatacgta cgatatcgcc ttctttggca acattgtttt catcatgagc tttgtatttt
    73321 ttagagtagt taatacgttt accatagact gggtggttac gttttgtttc aactacaact
    73381 gtgattgtct tgtccatttt gtcagataca acacgtccaa caagaacttt acgattattg
    73441 cgttccattg aaatttctcc ttccctagtc tattatttcg cttcagattg aactgttttg
    73501 atacgagcga tttgtttttt aacttctttc aagcgagctg tttgttccaa ttgaccagta
    73561 gcagcttgga aacgaagttc aaacaattct tttttcaatt cgttttcgcg cttcgcgagt
    73621 tcttcttgag aaagaccacg aagttcttta acaaattctt ttacttcatt aagtttcatg
    73681 ccttctcctt attctgcttc acgttttacg aatttacatt taactggcaa tttgtggcta
    73741 gcaagtcgaa gcgcttcacg tgcaatctct tcagatacac cagcgatttc gaacatcact
    73801 ttaccacgtt taactggtgc tacccaacct tcaggtgccc ctttaccaga tcccatacgc
    73861 acaccgatag ctttagcagt gtatgatttg tgtgggaaga ttttaatcca aactttacca
    73921 ccacgtttca tgtaacgagt catggcgata cgagcagctt cgatttggcg gttagtgatc
    73981 cagtggctag ttgtagcttg aagaccgtat tcaccgaatg ctacttcttt tccacctttt
    74041 gcttcaccgc gcatttttcc acggaattca cgacggtgtt taacacgttt aggtactaac
    74101 attggttatt tacctccttt agtgttttta cgagctggaa gaacttcacc acggtagatc
    74161 catactttaa caccaagttt accgtatgta gtatctgctt cttcccaagc gtaatcgata
    74221 tctgcacgaa gtgtgtgaag cggaacagtt ccttcagagt atccttcagc acgggcgata
    74281 tctgcaccgt tcaaacgacc tgatacttga gttttgattc ctttagctcc agcacgcatt
    74341 gcacgttgga ttgcttgttt ttgtgcacga cggaaagcaa cacgttgctc caattgacga
    74401 gcaattcctt cacctacaag gtgagcatcc aaatcaggtt gtttgatttc gatgatgttg
    74461 atgtgtactt gttttccagt caatttgtta agttttgcac ggagtgcatc aacgttagca
    74521 ccacctttac cgataaccat acctggttta gcagtgtgaa gtgaaacgtt aactttgttt
    74581 actgcgcgtt cgatttcaat agttgaaact gctgcgtcag caagttcttt ttgaacgaat
    74641 ttacggattg caagatcttc atgaaggtaa tccgcgtatt ctttttcagc ataccatttg
    74701 gcatcccaat cacggatgat gccgacacgc ataccaattg gatgtacttt ttgacccacg
    74761 attttacctc cttatttttc tgcaacagct acagtgatgt gagctgtacg tttgttgatt
    74821 ggtgaagctg aacctttcgc acgtggacgg aaacgtttca tagttggtcc ttcgtttgcg
    74881 aatgcttcag atactaccaa gttagcttta tccaaaccaa agttgttttc agcgttagct
    74941 acagctgagt tcaaaacttt caagatgatt tcagcagctt tgtttggagt gaatgtcaag
    75001 attgcgattg catcggctac gcttttacca cggatgttat caagaacaag acgtgattta
    75061 cgaggtgaaa cacgtactgt acgagccatt gctttagctg aagtaatttc tgccatttat
    75121 gttctcctta ttttctacgt gttttcttgt cgtctgcagc gtgacctttg taagtacgag
    75181 ttggtgcaaa ttcaccaagt ttgtggccta ccatgtcttc ttggatgtaa acaggtacgt
    75241 gtttacgtcc gtcataaact gcaatagtgt aaccaatgaa acttgggaag atcgttgatc
    75301 gacgtgacca agttttaata acttttttct tttcgtcgtt agcttgagct tcaacttttt
    75361 tcatcaaatg ctcatcgacg aaaggtcctt ttttaagact gcgtcccatt tttatatttt
    75421 ctcctttaaa tgttgtacca cagcggcttg cactcctatg gagcgctacc gagctggcgg
    75481 atttactagt tgcttaagcg actagtttaa tattatttct cgttgcgacg acgaacgata
    75541 agtttgtcag atttcgcttt cttgttacga gttttaagac caagagcagg tttgccccat
    75601 ggagtagatg gtgctttacg accaactggt gccttacctt caccaccacc gtgtgggtga
    75661 tcgttagggt tcattacaga accacgaact gttgggcgga tacctttcca acggctacgt
    75721 cctgctttac caaggtttac aagtccatgt tgttcgtttc cgacaacacc aactgtagca
    75781 cggcaagttc caagaatcat acgaacttca cctgattgaa gacgaacaag aacgtattta
    75841 ccttcagaac ccaatacttg agcagatgca ccagcagcac gtaccaattc accaccacga
    75901 cctggtttca actcgatgtt gtgaatcaaa gtaccaactg ggatgttagc aagtggaaga
    75961 gcgtttccga ctttgatatc tgcttctgga cctgaaacga tacgttgacc tacttcaaga
    76021 ccttttggag cgatgatgta tgctttcaca ccatcagtgt agtgtacaag agcgatgttt
    76081 gcagaacggt ttggatcgta ctcgattgtt ttaacaactg cttcaacgtt gtctttatta
    76141 cgtttgaagt caaccaaacg gtagaaacgt ttgtgtccac caccttggtg acgaactgtg
    76201 atacgaccgt tgttgttacg accagccttg ctcttcaatg caacaagcaa tgatttttca
    76261 ggagtgcttg ttgtgatttc agcgaaatcc aaagaagtca tattacggcg accgtttgtt
    76321 gttggtttat aaacacgaat tcccacgata tttcctcctt agattattca gcttcagcag
    76381 caaacaactc gattgcttta gaatcagctg taagtgtgat gatagctttt ttagttttgt
    76441 tagtaaaacc agtgtaacgt ccaacacgtt tagcttttgg ttttacgttg attgtgttaa
    76501 cattggcaac tttaacacct tcgaaagcag cttcaacagc ttgcttgatc aaaagtttgt
    76561 gtgcacgagt gtcaacttca aatacatatt tccctgcttc aagttgagcc attgagcttt
    76621 cagtgatgac aggttttttg ataacatcat acaaattcat tatgcaagaa cctcctcgat
    76681 tttagagata gctgcttgtg tgacaagaag tttgtcgcta tttgcgatgt caagaacact
    76741 tgcagttgta gcagttgcaa ctttcacgtt tggaaggtta cgagctgaaa gagctgcgaa
    76801 ttcatttcct tcttcaagga taacaagaac tttagaatcg atgctcaatg ctgcaagaac
    76861 ttttgcaaat tcagcagttt ttggagctgt aaatgaaaga gcgtctacag ctacgaattt
    76921 gttttcagca actttttcag agtaaactga tttaagagct aggcgacgaa ctttttgtgg
    76981 aagtttgtag ccgtatgaac gtggagttgg tccgaagaca acaccaccac cacgccattg
    77041 tggtgagcgg atagaacctt gacgagcacg tccagttcct ttttgacgcc atggtttgcg
    77101 tccaccacct gatactgcag agcggttttt aacagcgtgt gttccttgac gaaggcttgc
    77161 gcgttggctg atgattacat caaacacaac tgattcattt ggttcgatac caaatactgc
    77221 atcgctaaga acaacttggc cagcttcttt accagtttgg tcaaataatg ttacgtttgc
    77281 cattgtgact gatttcccct ttctttatta tttaccagct ttaactgctg atttgatagt
    77341 gataagagat ttcttagcac ctggtacgtt acctttgata aggataacgt tcttttctgg
    77401 aacaacttgt acaacttcaa ggttttgaat tgttacgcgg tcgccaccca tacgtcctgc
    77461 aaggttttta cctttgaata cgcggttagg tgcaacaggt cccatagaac ctggacgacg
    77521 gtggtaacga gaaccgtgag ccattggtcc acgtgattgt ccgtggcgtt tgataacacc
    77581 ttggaaacct ttacctttag aagtaccagt tacgtcaaca acgtccccag ctgcgaatgt
    77641 ttcaactgta atttcagcac caacttccaa gccttcaacg tttttgaatt cacgaatgaa
    77701 gcgcttagga gccgtgttag ctttcgctac atgtccttta gcaggtttgt tgctcaatac
    77761 ttcgcgtttg tcatcgaaac caacttggat agcgttgtat ccgtctgttt caacagtttt
    77821 aacttgaaga acaacgtttg gagttgcttc aataactgtt acagggatca attcgccagc
    77881 ttcagtgaag atttgagtca ttcccacttt tttccctaag attccttttg tcatgagaaa
    77941 atagttcctt ttctatattt ttattcaaaa agtttttaac gagcgttttt tatgctcaag
    78001 tctaagatac aaagggcgtc aaactcaacg tgaaaatagg aaactgacac agtgtctagc
    78061 agacgcgagg aagtttatct ttttcacacc aagtttagcc cgtgttcaat tagattgaaa
    78121 caccagcctc tgctctctta tattttagat taaagtttga tttctacgtt tacaccactt
    78181 ggaagatcca atttcatcaa ggcatcaact gttttttgag ttgggttaac gatatcgatc
    78241 aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagagt ctttgtattt gtgagtcgca
    78301 cgaatgattg tgtagaggct acgttcagtt ggaagtggga ttggacccgc aacttgtgca
    78361 cctgtacgag tagctgattc tacgattttt gcagccgctg tgtcaagcgt acggtgttcg
    78421 taagctttca aacggatacg gatttttttg tttgccatct ttttctcctt ttcgtctatt
    78481 taagataata ggctagctcc acaagaaaac cgacgcggtg ttgcgtggca atgcaaccga
    78541 gcgtgtcgca acctcttgca tcaaaactaa ggctgtaatt tacagcacca taatagaata
    78601 acacaaatcc cctgcgattg caagggattt gttaggtttt ttcgtttttt tgggatgaaa
    78661 ctgttttcct actttttctt tctaaaatta gggattttct tctatatttc ttctatacat
    78721 aagttaagtc ctcttgacat caaatttatc ttctgattgg tccactaaga tatccgcctt
    78781 agactcagtt tctttatagt atcgcagata ctgctcccgt ctcatctgat ggctagccaa
    78841 tataaaggat gcatcgcgat ttctcacagt cgtatctcta gcaaggcgtc gctttaattc
    78901 ggtctcctca tccgtgtaga aacagatggt tttttcaaag agttccttgg atagaaagcc
    78961 aacagacatc ccttcgacaa tcaaaattgg tttggctcca gacaagacct cactagcctt
    79021 ccaaggttct tcaattgtca agacatccat acccgcctgc aaggcaagga tatctctctg
    79081 caaactctcc agttcatgcg ccactggcag actggctgtc accttttgat tcggcgcgtc
    79141 cttgggtact accaaatagc gtccggaggt gatataggga tctgtttcta gcaaatttac
    79201 tgtagtagaa tctaaagctt ggtacaattc ctgtgcaaag gttgatttac ctgaagctcc
    79261 atgaccgtat attcccagtg tcctgacttt ccccgtctcg atctctgaga ctagttggtc
    79321 tactaagtct tttttcttca ttctctcttc acctgttcat agctttcttt tccgtcattg
    79381 agcttgtccc aaatcactac ttgcccactt ttgagcgatt tttgcacatc gataattcgt
    79441 tggttggacg aacctcgaaa ctggagcatg agatttctct tagttcgatc gtatcgtcca
    79501 tcgacaagaa tgtcaatcaa tgataagagt tccagtttat ctggagtttc caacatcatt
    79561 tcttcccaag tgtagcccgt ccaggaccaa atgtccttgt ctggcaattc ctttcggatg
    79621 cgtttaacta gaggcaagag aatgccagta ttgagaaaag gctcccctcc cagcaaagtc
    79681 aagccttgaa catagggttg ggcaaggtct gccataatct gctcttctaa ttctgctgta
    79741 tagggaatgc cagcattaaa agaccaagtc gcaacattat aacatccctc gcagtgaaac
    79801 atacagcctg atacatagag agagttgcgc acgccttcgc cgtccacaaa gttaaaggcc
    79861 ttgtagtcaa tgatacgacc ttgactaagt tcctcgcttt tccattcttg tggttttgga
    79921 ttattcattc gctacctcta tccaataacg ctcgactcca ttgcgagcat cctcaaatat
    79981 tccaccattt gctagaatga ctgctctgct agcaggatta ttcacgctac aggtcaccag
    80041 agctttcttg atgttctttt ccttagcaac ttgcaagccc tgacggagag tctcttttgc
    80101 ataacccttg cctctttcag atggacgaat ggagtagcca atgtggccac cttcttctag
    80161 tagaaagtta ctgaggcgca accggagatt aagaaatcca actgcttgac ctttctcaga
    80221 aaaaaccact aactgaattg caggaaccca tccttcaggc agattaatcc ccatttcctg
    80281 ttcctgattg ctttctaacc agtcttcata cacaaagttc tctgtattcc agaaaccgcc
    80341 gtcgtgaggc gactgaaatt tttcaaactc tgtcatcatc tctaaaactg tttctttatc
    80401 tgctaatctt ggtctgcgta attccatccc tacctcccac tatgagaaaa gcgagagctg
    80461 gctctcactt ttattttttc ttgttcttgt ttaaaaattg ttctctgaag ctaagcaagc
    80521 gttctttctt accagctcca cctttagagt gtttctcgtg ataacgggtc acttgtgcgc
    80581 gccccttatc gtctagttga tattttccca tttcatttct ttctaatttg ttacttgatg
    80641 cccagctatt ttaatcgttg aaccattcat atgtttgaca cgcgcagcga tttccttgtg
    80701 acgcccgtta accattggtc tcgcttgagg attgccaaga tagccacaag tccgtttaac
    80761 aacgtctact gttttaggat cgctattgcc acagtttgga caagcaaacc ctctctcagt
    80821 tggttcaaaa tccccttcaa agtcacactt gtagcaacgg tcaatcggag tattggtgcc
    80881 tagatagcct acacggtcat aagcataatc ccagacagct tccaaggcct ttggattttg
    80941 ctgaaggact ggatactcac aataatggat gaaaccacct gacgcacctg cttctggata
    81001 gactttctca aagtccaatt tttcaaacgg tgttggattt ttacgaacat cgtagtggaa
    81061 agagttggtg tagtattcct tgtctgtgat atcaggaata gagccaaact tgtctatatc
    81121 tagtcggcag aaacggtctg tcagactttc ggatggtgtt gagtagatag agaaatggta
    81181 gccatattgg tctgaccact cttctacacg gcgtttcata tcgtgaatga tgtctagcgt
    81241 gaattcctta gcatctggat tactttccca gctgttacca aagaaaactg tcgctacttc
    81301 atacaagccg atatagccca gcgaaacggt cgcacgacga ttcttaaaga gctggtcaac
    81361 actttcttct ttacctagac gatggccaaa agcaccgtac tgataaagaa taggagcatt
    81421 cgctggtgtc gcctctttag tgcgttcgac acggtaaaca agagcatctt ctgcgatatt
    81481 cattcgctcg ttgaagattt cccagaactt attcatatca ccttcagact caagagcaat
    81541 acgaggcaga ttaaccgtca caacacccag attcatgcga cctgaattga cttctacacc
    81601 attttcatcc ttccaccctt gaaggaaaga acggcagccc ataggcacct tgaaagaacc
    81661 tgtcaaatca acaatcttat cataagacaa gacgtctgga tacatccgct tggttgcaca
    81721 ctctagagcc aactgcttga tgtcatagtt gggagttcct tcctctaagt tgaggcctct
    81781 tttaagcgta aagataagtt taggaaagat agccgtacgg tgttctgaac caagaccctt
    81841 gatgcgaatg tttaaaatag ctttttgaat ttctcgttca aaacgactgg ttcccagacc
    81901 aaaacctaac gaagtaaaag gtgtttgtcc atttgaagtg aagagagtat tgatttcata
    81961 ctcaagagat tgcatggcat cgtagatgtc cttttgcgct ttcttccaag cgtaatcttc
    82021 ctgtttttca ggcaataccc actcttctgc atctttgaga tgtttttgat aattcttctc
    82081 tgcataaggc gccaaaattt catcgatacg gtcagctgaa cagccaccgt actggctaga
    82141 agcaacgttg gcaatgattt gagaaatctg tgctgtcgca gtctggatag acttgggact
    82201 ctctacctct gcatttccaa tcttaaaacc attttccaac atacccttaa aatcaatcaa
    82261 acagcagttg gtcatagggg tatagggact gtagtccaaa tcgtgatagt ggatatcccc
    82321 cttttggtgg gcattggcta cgtgcttagg aagcatttgc agtccgattg atttcccaac
    82381 aatccctgct gtcaaatcac gctgagtgtt aaagacatca ctgtctttat tagcgttttc
    82441 attgacaact gtctggtctt tgttgagaag tttatgaata ctaaagttga tatccgtcgc
    82501 ttttgagcgc tcaaaatccc tctgtgtccg ataagtaata tactcctcag ccagcgcata
    82561 ttctttggct tcaaggagtt catgttctac gatattttga atttcgtaaa tcttaattcc
    82621 ctgtggaaag cgactatgaa tttctgtaat aattcgctca gtcagatcat tgaggcgttt
    82681 ttcaaccagg ggtgtcacat ccataacctt gtcagccgcc ttgtggagag ccttgtcaat
    82741 cttgtccaca tcaaatacaa cacgtctccc atctcgtttc tcgacgaaga gagttggcaa
    82801 aattgtaatt ttttcttcta gtgcaatcat atgcatgctc ttttctaaaa tgttctttct
    82861 aaaaagtatt ataccactct aaaaagaaaa atcaatatct tgtgttaaaa atcctaaaat
    82921 ttttaaaaat acacaagata ttgatttggt tatttaattt tatagacttt aaactctgag
    82981 taatcagtct ttacttgttg gtaattttct tttaagagtg tttccacttc agaccagact
    83041 accaccttat catttaccac aatcacctta ggttgttttt ctttcaagtc attaagtaac
    83101 ttattacgat tttcctcagt tgctgtatag tgcaacgggg acaaaatcgc cgatggcgac
    83161 aagcgctcac tcttacgata aagagtcgca gtatcatccc atgcatagat agcatcttcc
    83221 gtatttgtct cctcttttac caaatcagca agacgattac gttcttgata aggtactgga
    83281 taaaggacaa acttcatcaa aaaaggaaca gacaaaagat agacaaaccc aaaaactagc
    83341 agatatagat ttcctttgaa gaaacgactc cataggctaa ctggcgtttc tcttcggctt
    83401 cttctagcac tacgccccct ctgttgactc ttaatattgg ttaccaataa aagtaggaaa
    83461 acagggaaga cgaccatcaa ataagaagcg tgcagaggct cttgtgaaaa gaaaagtaaa
    83521 atcaaaccta aaattaccac aagacttgca ggaaccgaaa tgacgtatag tttaaagggt
    83581 ttggattgaa agagacctaa aaagatacaa accacaaatc ccaaaccaag ggacaacaag
    83641 ccataaaaaa acatattctc taaaatttta gaagtaaaat caaagcgaat actgtcaata
    83701 ggataacgaa taccactaat cgcatcccca aaacttcctg ttgcagcact atagtaggca
    83761 gttggataaa agacaagtga aaaacctaaa gccactgcaa gaaactgata aaacccatgc
    83821 gcaaagcgtc tatgcccaag gttaaagacc aacaagccta aactcactac agcaataaac
    83881 aggagcgatg ataagggagc aaagaaaaat ccgcctgcca aagctagccc aatccgtaca
    83941 aatcccttat catggcttgg attgcttagg taagccgcaa ctaaactaaa ggctgcgaat
    84001 aagaaaggaa gcgctaaaag agtcgcatag cctccaccaa aagcaagacc tgtaactagc
    84061 atgtaaaaaa tagtcaccag atgtccagct tggtctcctt gctctgtcaa ggtgtccgct
    84121 gatctaaaaa ggaaaaatcc tcctgctacc aaggctaacc actcaaagat ggcaaagaaa
    84181 aatccgccct gactcacgta agtcagcaaa taataaagca aaccttgact tccataatag
    84241 tcgctgtaaa tcttccctgt ctgatgaagc gcccaacctg cataaaaatc ctgcacttct
    84301 tgtgcactca ttaagtcgag taatagcggt actcctagag ttatccccgt tacaagcgta
    84361 ctccatagta aaattttcac caaaggaaga cgacttgatt cacgatgatg cgattcttgt
    84421 tcgatttggt attctagagg ttcacgattc tccttatgaa cttcttctac tctaccatac
    84481 acactcatat cgtttctcct gttcaattta tctgtcttag tatatcaaaa agtcctagga
    84541 ttgtcagctt gcgatggctg tttgagtgat tttttgcata gtttcacaaa ggtttcaatt
    84601 tcccgaaagc ggtgtaaatg tgtctgtttg aactcaatca tatcattcac ttcttcctta
    84661 gttagcatga attcccctcc tctttctttc tattcgtaaa agacaagaaa aataggtctg
    84721 gtttgtatcc taagttctag atttcttata agataggggc gaatagctgg gcgatttctt
    84781 ttacaagctt ttgataccag ctcgttttga tagaatgagg atagacttcc tgagaatctg
    84841 caaatatatt tcgaaaatct cgggcgattt cccttataga aggagtttta tagagtaaga
    84901 ctgcattttc ataatggtgt accaagctcc ggtagtcgag attgattgtc cccaccaccg
    84961 caaaatcttc gtctaccaac atctgcttac tatgaataaa acctggacta tactcataaa
    85021 tccgaacacc agcagaaaga aggtcgggat aagctcctct cgtgactaac tgaatgaact
    85081 tcttatctgg tatgtaaggg gtgataattc gaacatcgac ccctctcata gctgcatttt
    85141 tgattgtctc tgttaaatca taatctataa tcaaataagg tgtcgtaata tagaccgatt
    85201 ccgttgcttg attgattaaa ctctgataaa cttttttccc tacctgcgct cgaaaaattg
    85261 gcttgggtcc acttccgtat ggaatggtta aaccgtcact cgggatagaa tgattttcta
    85321 aatgatattg atcaaaatca ctaatttctc ctcgattaat gtaccaagtg gtcaaaaata
    85381 agcgtgtcag ggcttttact gctagtccgt ctaagcgaat tccactatcc ttccaataac
    85441 caaatctctc gacgtggtta atgtactcat ctgccagatt gaccccacca gtataggcta
    85501 tctgaccatc aacaatcaat atttttctat gatctctgtt attataagcc actgtcaaac
    85561 gaggaataac tttattgaat ttatgggcct cgatgcccag ctgacgaagt cgatgtgcat
    85621 aatctcctgt taaagtagcc atacagccga tatcatcata gagcatctta acctctacac
    85681 cctgagctac cttttgctct agtatatcta gtatgcgatt ccacatcaaa ccttcttcta
    85741 taatgtaata ttccaagaaa ataaatttct cagccttttt aagatcttct accatctttt
    85801 tccacatagc ttctccgtta ggaaaaaatg tagaggcagt ttgatcatag atgtcagcat
    85861 tggtatccat actcaataag gacttgatga ctccataagc tgccttgtca ctttctttta
    85921 atttctcttt tagtagctgg ctatttgctt cttggaaatg catagagcct agcttcttca
    85981 gttgtttgat ttcttttttg gacaatcgcc tttcaccaaa catcagatag agcaagggac
    86041 caaatactgg cacaaaggct actaacaacc aggttacctt attctcagga gtcgtattac
    86101 ggttgactat cgaaatgatt gtgatgatac tcagtagaat gaggacagta atccataaaa
    86161 tgggagccat gcgtcctaaa taaagaaaga gaccaaaaac aagacaaagt tcaagtagca
    86221 taatcgaaag actaaagcca tacttggaca tcaataattg aaatttccta tatttcatat
    86281 cccctccttg atagtttgaa tgctaaaaac aagtaattga tactagtata acgcaggtat
    86341 tcggtagaag gcaagtattt atgattattt tctaggtaaa agtaaaaagg ctgaaaaatc
    86401 cagccctttc tacttgcata gtcttattta gttaaaacga tttggtccga tgggtctcta
    86461 tccatatcat ctacgacgat ttgattaccg tttacagtgt aaattttgac atcatcgcca
    86521 ataatcatgc gttgatttgc tgaatcaaat gtaacctgtt tgactttctg atccccgtca
    86581 gattcgagct cagtccatgt accagtcgtt ccagttacca ctaaagtgat acggtctccg
    86641 tcatcctgac cagtataagt cccatcaata tcggtcggtt gagcaacagg ttggttttgt
    86701 actggagccg cttgattttg gttacctcct gcatttgcag tattggtatt ctgttgagca
    86761 ggctgttgga cctgctgagc aacaggctgt tgaacctgtt gagctctttg tgaacaagct
    86821 actaagagac taaaacttgc tagagaagca agagaaagtg tgaatctttt taatttcatg
    86881 atgaatttcc tttctgctac caatttagag aaattttctc taaccagcaa ttcccctagt
    86941 ataacaagtt caaaaaaggg ggtcaattta tctgctcacg gtccagcagg tagccccgta
    87001 cttctgagat aaaatagaga gaccctgtaa cgaacagcaa gtcttgagcg tctgcccttt
    87061 cttcgaaact gctgataaat tctcggtaag agggaattac atggtaacct gttacatctg
    87121 tctcgtccaa agacccttga tagtcaaagc cggtcatttt gagttccact tgaggtaaat
    87181 tttcagtcag ataacccaac atcccttgat aatccttacg tttcaaggat ccaaagagga
    87241 tttgaggtcg atagccttcc tgctcttttt ctttgataaa ctcaaccaag cgagtcaagg
    87301 cagggaggtt atgagcacca tccaagtaaa tctgtgggcg aatacgctcc aagcgaccag
    87361 cccaatgggt ctgttccaag gcctttctta cagcctgttc atcaatagct tcctttcttt
    87421 ctcccataaa aagaagaaaa gtttgcaacg ccaaggccgc attctcctgc tgataggctc
    87481 cttctaagcc tatgttcagc tgtgaaagat ttagtaaaga gcttgaaaaa tcaccattca
    87541 gcactgaaaa atcttgacct gcctgataaa ggtcaacagc taaagattcg gcttttttct
    87601 gacagacaag tctagcttct ggaggcaatt tcgcaatcac tgccttttta ccagccttga
    87661 aaataccagc tttctgctct gcaattgctt ctagactatc acccaaggtt tcttgatggt
    87721 caagcccaat ggaggtgatg acagcaagct ctccagttac cacattggtc gtgtcaagta
    87781 agccaccaat tcccacttct aataaaacca aatccacctc ctgctcccta aaataaagaa
    87841 aagcaaccaa ggtcagcaat tcaaaaaagg acaactggtc aggagtttgc agaagcgttt
    87901 tctccatctc ctttacctga tcagccaaac ggataaagtc tgcgtctgct ataggttgcc
    87961 cattaatgca gattcggtca ttgatagaga cgatatgagg ggaggtaaag gtcgcaactt
    88021 ttttgccatg ccccataaat aattccctca taaaagcaat ggtagatcct ttcccattag
    88081 tccctgttac gtggataata gggtaagact gctcaggatt tcctaacaaa tccaccgctt
    88141 gctgcattcg gcccaaacct gatcgaaaat ttaaaccaat ccgactatgg agccattctt
    88201 ctacttcaaa catacacatc tccttgacaa aagtccaatc aattatcgca ttaaagtatg
    88261 gttactaata aaaacaaggc caggattttc gtcccgacct cttacctggt tagctaataa
    88321 ctagccacta tgaatgtgaa tatgggctaa aaacatccac tagatgttcc aactcttctc
    88381 aatttctaag agttggggta aaaatgttca ctggacgttc caactcttcc ccatttctgg
    88441 gagttgggct gatacagtct cccagactta ccagctctat cttatttaaa ggagctgggc
    88501 taaaaacgtc cccccgaacc taccagttct ctcttattta aaggaactgg gctaaaaaca
    88561 tccactggac gttccaactc ttccccattt ctgggagttg ggctgataca gtctcccaga
    88621 ctgtatcact cctccataaa gctgttgaag acttcttcaa tcatgttcca ttcgtcttct
    88681 gagtcttctg ggattggttg caattcgcct tctgttccat cttcgttttc gatgaatgag
    88741 taagcttgga tttcaacttg tccgtcttcg tcttcttctg cgttaactgg tactagaaga
    88801 acatagtttt taccaaattc ttcttttcca tcaattgtca aaaggatttc aaacaaggtt
    88861 tcatttcctt gctcatctac tagtgtgatt agttcacgtt cttcgtggtc gtggttatga
    88921 tcgtgtgaca tagcctctcc tttatattaa aattttctat ctaaataatt ttgtaaaatc
    88981 agctgagctg ctaacttatc aatgactttc ttgcgcttat tgcgactgat atctgcttgt
    89041 tcaatcaaca tgcgctcagc agccactgtt gtcaagcgtt catcctgata gtctactggt
    89101 aaaccaaaaa actcttctag ctttgctccg taggcttgac tagcttctac gcgcggtcca
    89161 cttgtattgt tcatgttttt aggcaagccc actacaaatc gttccacctt gtaagtatca
    89221 accaattcct taacgcggtc aaaaccaaat tggccttgtt cttcatttat ctggatgatt
    89281 tcaagccctt gagctgtaaa accaagcgga tcgctaatcg ccacccctac cgtttttgaa
    89341 ccgacgtcca atcccataat tctcataggt tatagatcga ctccttgtcc tttgaggtag
    89401 taacgaacca attcctcaac gatttcatca cgctcatact tacggatttg atttcgtgca
    89461 ttattataac gaggaacgta ggcagggtct ccactcaata cgtaacctac gatttggtta
    89521 attgggttgt aacccttatc gttcaacgaa gcataaacat ctgtcaaagt ttcgctaatt
    89581 tcttttttat tggaatcgtc caatttaaaa cgtactgttt cttcagtaaa tcccattcta
    89641 acaccctctt tccttagaat agtaccatta tagcataatt ccttaccttc tacaattcag
    89701 gcagtctatt tatttggatt ttctattgtt ctgtcgcgcc atttgccaat ctatctgaaa
    89761 tatatttgct tggttcattt ttcaaaagat tttccaaacc aatattcttc agatgttcca
    89821 actgggaagc cttcttgaca tccagaactt gaaaatcaaa actagtcgtt gtttgaagtt
    89881 ccgttgcgct caatagtttt gtttcaagtt tgaaacctgc caatttacga gcttcaatga
    89941 tagacttatc cttctcctcc gcttcaagaa gagctttttg agtttcctcc actccatgtt
    90001 ggtcaatata agtcttcaac tcatcagaga ctggacgttt ttgttggata gttagactca
    90061 aaaaagtctt gtctttgtaa gtaattgttt gtgtctgctg gctaccatca tctgacttgg
    90121 gcaagaccaa agtcttagtt acaactgtat tcttctcagc attttcaata actggcaatg
    90181 ccgactgaag cgtatctttt tctgtttttg tagctggtcc agtttctttt ttctgtccgc
    90241 aaccaaccag gacaaaaagg aaagctagac taacaagaac tatttttttc atttctttct
    90301 tctttctttt tgaaattaaa atagaataag actgggaagt gctcccagcc ttgatgttta
    90361 tagagctgca cgcaaacgtg cttctgcatt ttctacatta cggacagagc gtggtaggaa
    90421 ggcacgaata tcgtcttcct tgtagccaac ttgcaggcgt ttttcatcta caaggattgg
    90481 gctctttaaa attctcggtg tttccataat cagattgaga acttcattga cactcaaatc
    90541 ttcaatatcc actccaaggg ctttggcata gcgattttta gacgaaacga tgctggctat
    90601 tccgttatct gttttggtta gaatatccag taattcttct ctcgtaattc cttctttacc
    90661 aaggttttgt tctttataac ttaactggtg ggcattgagc caggtttttg cttttttaca
    90721 gctagtacaa cttgagactg tataaatttt aatcatgtac ctaccccttt cgctacatgt
    90781 tactatcagt ttagtctatt ataccataaa aaacatccga cttgcgacct atttttaat
//
LOCUS       DAVDWM010000004        90084 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVDWM010000004 DAVDWM010000000
VERSION     DAVDWM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 90084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.004"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..255
                     /locus_tag="VUW49_000318"
     CDS             <1..255
                     /locus_tag="VUW49_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET7980295.1"
                     /translation="IRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNP
                     MELLTIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            590..1324
                     /locus_tag="VUW49_000319"
     CDS             590..1324
                     /locus_tag="VUW49_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980296.1"
                     /translation="MRIKEKTNNINGGIKNVSKHYGHSIILKDINFALNKGEIVGLAG
                     RNGVGKSTLMKILVQNNQPTSGNIISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGV
                     DYNQERFRCLIQELDLTQSINKKVKTYSLGTKQKLALLLTLVTEPDILILDEPTNGLD
                     IESSQIVLAVLKKLALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHN
                     FLFEIAFSSATDRDIFITKQEFWDIV"
     gene            1484..1964
                     /locus_tag="VUW49_000320"
                     /pseudo
     CDS             1484..1964
                     /locus_tag="VUW49_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2349..2558
                     /locus_tag="VUW49_000321"
     CDS             2349..2558
                     /locus_tag="VUW49_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7980297.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2548..3036
                     /locus_tag="VUW49_000322"
     CDS             2548..3036
                     /locus_tag="VUW49_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980298.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            3152..4612
                     /locus_tag="VUW49_000323"
     CDS             3152..4612
                     /locus_tag="VUW49_000323"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET7980299.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            4609..5433
                     /gene="nadE"
                     /locus_tag="VUW49_000324"
     CDS             4609..5433
                     /gene="nadE"
                     /locus_tag="VUW49_000324"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET7980300.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            5783..6334
                     /locus_tag="VUW49_000325"
     CDS             5783..6334
                     /locus_tag="VUW49_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980301.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKEDPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            7049..7741
                     /locus_tag="VUW49_000326"
     CDS             7049..7741
                     /locus_tag="VUW49_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET7980302.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            7795..8184
                     /locus_tag="VUW49_000327"
     CDS             7795..8184
                     /locus_tag="VUW49_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET7980303.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(8845..9873)
                     /gene="queA"
                     /locus_tag="VUW49_000328"
     CDS             complement(8845..9873)
                     /gene="queA"
                     /locus_tag="VUW49_000328"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7980304.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVHFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            10024..10725
                     /locus_tag="VUW49_000329"
     CDS             10024..10725
                     /locus_tag="VUW49_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET7980305.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLL"
     gene            10871..11047
                     /locus_tag="VUW49_000330"
     CDS             10871..11047
                     /locus_tag="VUW49_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7980306.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            11177..12112
                     /locus_tag="VUW49_000331"
     CDS             11177..12112
                     /locus_tag="VUW49_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET7980307.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            12105..12893
                     /locus_tag="VUW49_000332"
     CDS             12105..12893
                     /locus_tag="VUW49_000332"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7980308.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGFRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            12894..13277
                     /locus_tag="VUW49_000333"
     CDS             12894..13277
                     /locus_tag="VUW49_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET7980309.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            13293..13682
                     /locus_tag="VUW49_000334"
     CDS             13293..13682
                     /locus_tag="VUW49_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7980310.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            13766..14896
                     /locus_tag="VUW49_000335"
     CDS             13766..14896
                     /locus_tag="VUW49_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET7980311.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            14901..15638
                     /locus_tag="VUW49_000336"
     CDS             14901..15638
                     /locus_tag="VUW49_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET7980312.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            15649..16422
                     /locus_tag="VUW49_000337"
     CDS             15649..16422
                     /locus_tag="VUW49_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET7980313.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            16412..17029
                     /locus_tag="VUW49_000338"
     CDS             16412..17029
                     /locus_tag="VUW49_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET7980314.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            17239..17640
                     /locus_tag="VUW49_000339"
     CDS             17239..17640
                     /locus_tag="VUW49_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET7980315.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNITTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            17640..17918
                     /locus_tag="VUW49_000340"
     CDS             17640..17918
                     /locus_tag="VUW49_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET7980316.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            17908..18681
                     /locus_tag="VUW49_000341"
     CDS             17908..18681
                     /locus_tag="VUW49_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7980317.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRSLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            18682..19986
                     /locus_tag="VUW49_000342"
     CDS             18682..19986
                     /locus_tag="VUW49_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET7980318.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGHLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            20560..21438
                     /gene="pstS"
                     /locus_tag="VUW49_000343"
     CDS             20560..21438
                     /gene="pstS"
                     /locus_tag="VUW49_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7980319.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            21438..22355
                     /gene="pstC"
                     /locus_tag="VUW49_000344"
     CDS             21438..22355
                     /gene="pstC"
                     /locus_tag="VUW49_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7980320.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            22345..23229
                     /gene="pstA"
                     /locus_tag="VUW49_000345"
     CDS             22345..23229
                     /gene="pstA"
                     /locus_tag="VUW49_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7980321.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVIHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            23240..24043
                     /gene="pstB"
                     /locus_tag="VUW49_000346"
     CDS             23240..24043
                     /gene="pstB"
                     /locus_tag="VUW49_000346"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET7980322.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            24056..24814
                     /gene="pstB"
                     /locus_tag="VUW49_000347"
     CDS             24056..24814
                     /gene="pstB"
                     /locus_tag="VUW49_000347"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET7980323.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            24826..25479
                     /gene="phoU"
                     /locus_tag="VUW49_000348"
     CDS             24826..25479
                     /gene="phoU"
                     /locus_tag="VUW49_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7980324.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            25636..26451
                     /locus_tag="VUW49_000349"
     CDS             25636..26451
                     /locus_tag="VUW49_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7980325.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQVGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(26471..27742)
                     /locus_tag="VUW49_000350"
     CDS             complement(26471..27742)
                     /locus_tag="VUW49_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7980326.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            27884..28615
                     /gene="budA"
                     /locus_tag="VUW49_000351"
     CDS             27884..28615
                     /gene="budA"
                     /locus_tag="VUW49_000351"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET7980327.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            28625..29377
                     /locus_tag="VUW49_000352"
                     /pseudo
     CDS             28625..29377
                     /locus_tag="VUW49_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            29489..30439
                     /gene="murB"
                     /locus_tag="VUW49_000353"
     CDS             29489..30439
                     /gene="murB"
                     /locus_tag="VUW49_000353"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7980328.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            30498..31655
                     /locus_tag="VUW49_000354"
     CDS             30498..31655
                     /locus_tag="VUW49_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980329.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            31636..32442
                     /locus_tag="VUW49_000355"
     CDS             31636..32442
                     /locus_tag="VUW49_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7980330.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVALLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            32439..33212
                     /locus_tag="VUW49_000356"
     CDS             32439..33212
                     /locus_tag="VUW49_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7980331.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            33209..34279
                     /locus_tag="VUW49_000357"
     CDS             33209..34279
                     /locus_tag="VUW49_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7980332.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            34532..34771
                     /locus_tag="VUW49_000358"
     CDS             34532..34771
                     /locus_tag="VUW49_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980333.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            35046..35531
                     /locus_tag="VUW49_000359"
     CDS             35046..35531
                     /locus_tag="VUW49_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET7980334.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            35553..38171
                     /gene="alaS"
                     /locus_tag="VUW49_000360"
     CDS             35553..38171
                     /gene="alaS"
                     /locus_tag="VUW49_000360"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7980335.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(38261..39715)
                     /locus_tag="VUW49_000361"
     CDS             complement(38261..39715)
                     /locus_tag="VUW49_000361"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET7980336.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            39894..40643
                     /locus_tag="VUW49_000362"
     CDS             39894..40643
                     /locus_tag="VUW49_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980337.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGDIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            40624..42258
                     /locus_tag="VUW49_000363"
     CDS             40624..42258
                     /locus_tag="VUW49_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980338.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSSMIAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            42945..44108
                     /locus_tag="VUW49_000364"
     CDS             42945..44108
                     /locus_tag="VUW49_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET7980339.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFNVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASHTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            44105..44782
                     /locus_tag="VUW49_000365"
     CDS             44105..44782
                     /locus_tag="VUW49_000365"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET7980340.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            44772..45626
                     /locus_tag="VUW49_000366"
     CDS             44772..45626
                     /locus_tag="VUW49_000366"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7980341.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            45645..46712
                     /locus_tag="VUW49_000367"
     CDS             45645..46712
                     /locus_tag="VUW49_000367"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7980342.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            46722..47888
                     /gene="aroC"
                     /locus_tag="VUW49_000368"
     CDS             46722..47888
                     /gene="aroC"
                     /locus_tag="VUW49_000368"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7980343.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            47898..49001
                     /locus_tag="VUW49_000369"
     CDS             47898..49001
                     /locus_tag="VUW49_000369"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET7980344.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            49012..49350
                     /locus_tag="VUW49_000370"
     CDS             49012..49350
                     /locus_tag="VUW49_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET7980345.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            49442..50725
                     /gene="aroA"
                     /locus_tag="VUW49_000371"
     CDS             49442..50725
                     /gene="aroA"
                     /locus_tag="VUW49_000371"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7980346.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            50718..51194
                     /locus_tag="VUW49_000372"
     CDS             50718..51194
                     /locus_tag="VUW49_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7980347.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEETFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            51191..52039
                     /gene="pheA"
                     /locus_tag="VUW49_000373"
     CDS             51191..52039
                     /gene="pheA"
                     /locus_tag="VUW49_000373"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7980348.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYTEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            52036..53310
                     /gene="psr"
                     /locus_tag="VUW49_000374"
     CDS             52036..53310
                     /gene="psr"
                     /locus_tag="VUW49_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET7980349.1"
                     /translation="MSKENLLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            53589..54434
                     /locus_tag="VUW49_000375"
     CDS             53589..54434
                     /locus_tag="VUW49_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET7980350.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            54424..55527
                     /locus_tag="VUW49_000376"
     CDS             54424..55527
                     /locus_tag="VUW49_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7980351.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            55529..56515
                     /locus_tag="VUW49_000377"
     CDS             55529..56515
                     /locus_tag="VUW49_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7980352.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            56531..57964
                     /locus_tag="VUW49_000378"
     CDS             56531..57964
                     /locus_tag="VUW49_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET7980353.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            57993..59027
                     /locus_tag="VUW49_000379"
     CDS             57993..59027
                     /locus_tag="VUW49_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7980354.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            59169..59906
                     /gene="mecA"
                     /locus_tag="VUW49_000380"
     CDS             59169..59906
                     /gene="mecA"
                     /locus_tag="VUW49_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET7980355.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFAEDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            60057..61343
                     /locus_tag="VUW49_000381"
     CDS             60057..61343
                     /locus_tag="VUW49_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7980356.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            61345..62214
                     /locus_tag="VUW49_000382"
     CDS             61345..62214
                     /locus_tag="VUW49_000382"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7980357.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            62290..62367
                     /locus_tag="VUW49_000383"
                     /pseudo
     CDS             62290..62367
                     /locus_tag="VUW49_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            62509..63447
                     /gene="msrB"
                     /locus_tag="VUW49_000384"
     CDS             62509..63447
                     /gene="msrB"
                     /locus_tag="VUW49_000384"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7980358.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            63505..65229
                     /locus_tag="VUW49_000385"
     CDS             63505..65229
                     /locus_tag="VUW49_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980359.1"
                     /translation="MKYLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLIQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            65231..66979
                     /locus_tag="VUW49_000386"
     CDS             65231..66979
                     /locus_tag="VUW49_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980360.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            67281..68540
                     /locus_tag="VUW49_000387"
     CDS             67281..68540
                     /locus_tag="VUW49_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET7980361.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    68616..68751
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            68790..69290
                     /locus_tag="VUW49_000388"
     CDS             68790..69290
                     /locus_tag="VUW49_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET7980362.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNNFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            69366..69734
                     /gene="rplL"
                     /locus_tag="VUW49_000389"
     CDS             69366..69734
                     /gene="rplL"
                     /locus_tag="VUW49_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET7980363.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            69935..70108
                     /locus_tag="VUW49_000390"
     CDS             69935..70108
                     /locus_tag="VUW49_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980364.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQS"
     gene            70105..70353
                     /locus_tag="VUW49_000391"
                     /pseudo
     CDS             70105..70353
                     /locus_tag="VUW49_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            70367..70690
                     /locus_tag="VUW49_000392"
     CDS             70367..70690
                     /locus_tag="VUW49_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980365.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLW
                     EVSHD"
     gene            70683..71063
                     /locus_tag="VUW49_000393"
     CDS             70683..71063
                     /locus_tag="VUW49_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET7980366.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            71076..71219
                     /locus_tag="VUW49_000394"
     CDS             71076..71219
                     /locus_tag="VUW49_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980367.1"
                     /translation="MAERFWENLSIILAERNISCIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            71312..71899
                     /locus_tag="VUW49_000395"
     CDS             71312..71899
                     /locus_tag="VUW49_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980368.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            72300..73109
                     /locus_tag="VUW49_000396"
     CDS             72300..73109
                     /locus_tag="VUW49_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7980369.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            73114..73815
                     /locus_tag="VUW49_000397"
     CDS             73114..73815
                     /locus_tag="VUW49_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET7980370.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            73840..74757
                     /locus_tag="VUW49_000398"
     CDS             73840..74757
                     /locus_tag="VUW49_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET7980371.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            74785..76326
                     /locus_tag="VUW49_000399"
     CDS             74785..76326
                     /locus_tag="VUW49_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET7980372.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            76345..76803
                     /locus_tag="VUW49_000400"
     CDS             76345..76803
                     /locus_tag="VUW49_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7980373.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            76805..79027
                     /locus_tag="VUW49_000401"
     CDS             76805..79027
                     /locus_tag="VUW49_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET7980374.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            79067..80176
                     /locus_tag="VUW49_000402"
     CDS             79067..80176
                     /locus_tag="VUW49_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7980375.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            80333..81223
                     /locus_tag="VUW49_000403"
     CDS             80333..81223
                     /locus_tag="VUW49_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7980376.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            81248..81571
                     /locus_tag="VUW49_000404"
     CDS             81248..81571
                     /locus_tag="VUW49_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980377.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            81561..83552
                     /locus_tag="VUW49_000405"
     CDS             81561..83552
                     /locus_tag="VUW49_000405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET7980378.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            83568..84044
                     /locus_tag="VUW49_000406"
     CDS             83568..84044
                     /locus_tag="VUW49_000406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET7980379.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            84078..84659
                     /locus_tag="VUW49_000407"
     CDS             84078..84659
                     /locus_tag="VUW49_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980380.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            84724..85731
                     /locus_tag="VUW49_000408"
     CDS             84724..85731
                     /locus_tag="VUW49_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET7980381.1"
                     /translation="MDTNLFSKINTTISVKENDFITEEKFQKIIQSKDTETLAFILES
                     TPYHLSIDILEDPSQTEISLMTKLVNDYRWAYAESPSDIIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVREVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVEMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYG
                     S"
     gene            85721..86041
                     /locus_tag="VUW49_000409"
     CDS             85721..86041
                     /locus_tag="VUW49_000409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET7980382.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            86104..87879
                     /locus_tag="VUW49_000410"
     CDS             86104..87879
                     /locus_tag="VUW49_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET7980383.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            87880..89265
                     /locus_tag="VUW49_000411"
     CDS             87880..89265
                     /locus_tag="VUW49_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET7980384.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            89288..89899
                     /locus_tag="VUW49_000412"
     CDS             89288..89899
                     /locus_tag="VUW49_000412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET7980385.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT        27249 a        15827 c        19707 g        27301 t
ORIGIN      
        1 attcgtcaac gaaacgtcat taacgaaggg gaccaagttg agttttatgg tccaggtttc
       61 cgtcattttg aaacctatat tgaagatttg catgatgcta aaggcaataa aatcgaccgc
      121 gctccaaatc caatggaact attgactatt aaagtcccac aacctgttca atcaggagac
      181 atggttcgag ctcttaaaga ggggcttatc aatctttata aggaagatgg aaccagcgtc
      241 acagttcgtg cttaatgtag ttgtttagtt ttaaaaaact atgcaaagct ccatatacaa
      301 cacttaaacg agattaaaga atggcgaaat cccttgatgc gcaagagatt agctgtcttt
      361 tttatttttt aagtgataaa gtcggagttt aggcatcaaa gcctatcaaa ttaaacaaag
      421 aagcgatgtc ttagatattt tgaaaaaaat taataagcag aaaactctct attattttgt
      481 tgtagagagt tttttgttaa taaaatttca caaaatgaca tttatatatt gcattaagtt
      541 agatatatga tataatattg ttaaaaagag gcgcaacttt ttaaaattaa tgagaatcaa
      601 agagaaaacc aataatatta atggaggaat aaaaaatgta agtaagcatt atggtcattc
      661 aatcattctc aaagatataa attttgcact taacaagggt gaaattgttg gtctagcagg
      721 gagaaatgga gttggtaaga gtacgttgat gaaaattctt gttcagaata atcaaccgac
      781 ttcaggtaat attataagca gtgataatgt tgggtattta atcgaagaac caaaattatt
      841 tttatctaaa acaggtttag agaatttaaa atatttgtca aatttatatg gtgttgacta
      901 caatcaagaa agatttagat gtttgatcca agagttagat ttgactcagt ctattaataa
      961 aaaagtaaag acctattctt tgggtacaaa acaaaaatta gctttgcttc taactctcgt
     1021 tacggaacct gatatattga ttttagatga accgactaat ggtttagata ttgaatcatc
     1081 acaaatagtt ttagcggttc taaaaaaatt agctttacat gaaaatgtgg gaattttaat
     1141 atcgagtcat aaattagaag acattgaaga aatttgtgag agagttcttt tcttggagaa
     1201 cgggcttttg acatttcaaa aagtaggaaa agatagtcat aatttcttgt ttgagatagc
     1261 tttttcatca gctacagata gagacatttt cattaccaaa caagaatttt gggatattgt
     1321 ttaggaagag ggattgagaa ttactatgtc tgggaatatt caaaatagtg agctttttaa
     1381 attttttaac gaaaactcta ttaaagtagt tgattttgaa actaaaaaag agacgcttaa
     1441 agatatttac ctaaatcgtt caaaataaag gaaggttata atcatgaaat taaataaaca
     1501 gaagaatcgg atgatttacg tcttgtctaa ttttctatat gctatctcag tttccattat
     1561 ttatgctttg aatggcattg tgttactagt catagtaagt aaattgggta ttccaggtga
     1621 tttaggatta aattttatag tagctattgt agtcaataca attttgttag tcctgtttta
     1681 ttttctatta tcttacattt tctatttata caaattgaaa agtggcttgg tatttggtat
     1741 tttagtagct ttactactct ttatctctaa tatattaaat acgatgatga tgaatactag
     1801 taatgatttg tttatcaaag caattgaact tcttcctttt ttattcttta cctgtatttg
     1861 tggcttcaaa tacgatgtct attagtcagt atcttgtgct aatcactaca atcattttat
     1921 tgtacttttt cactctcaag aaaagcaaga agtattcatt ttagtttcat ttagtattat
     1981 tttgcatacc taaaatacag taaaaaatca gtcatcttgg tatgctcctg ctttcactat
     2041 tcaacacgtt tttgacttat actaggctca tttccaaaag cattatataa tagtgatatg
     2101 aaaccaacta aactaaacaa gaaatataag caataaaaat tcgtttaaaa gatcttacta
     2161 aagctaatac taaataaaaa taaaagagta aactaggaag tttatttcaa acaacctaaa
     2221 atactgattt tcggctgaag ataatactgg agtgcaaatt aatggggtta taataaatag
     2281 ctgatagctt gtgttggttt tggatttttt aagagtagat gagtattaaa actataagga
     2341 ggacgaaggt ggctaaaaat ttaaaattaa aattagctcg ggtagagcgt gatttaacac
     2401 aaggtcaact ggcagaggct gtcggggtga cacgccagac tattggttta atagaggcgg
     2461 gaaaatacaa tcccagtctc tcgctctgcc agtctatttg cagatgttta gggaaaaccc
     2521 tagaccaact attttgggag gaagaagatg aaaaatagat tttattatta tcaattacta
     2581 gacgaaagag aagaacaact gttcaataaa gcgggctctg aaagtttcta tatctgcatt
     2641 gctttgtcgc tcctatctta tatcatttca gtattagcac caagcctttt taattctaat
     2701 atgctgctaa tcgttatcat catagggaca ttttactttt tcaatcgtgc ccgttatctg
     2761 ggagtgacct actatagtcg ttttcatttt acgattttgg gttgtttttt cctaaccttg
     2821 gctattacgg ctcttttgat gttgcagaat tatcaattca acatagaaat ttatcagcac
     2881 aatcctttga attttaaata cctgtctgct tgggtcatta cttatatcat ttaccttccg
     2941 tggatcttta ttggcaatct tggtcttaag agctatggcg aatgggctca gaaaaaattt
     3001 gaacaagata tggatgaatt ggagagtgga gaatagcttg ttactctttt ctcaatccag
     3061 ctaaaatgtg atataatagt actaatttat tggaatacat gaaagttctt gaaaattttc
     3121 atgggtttct agctaaggaa gtaggaaaag tatgtatcca gatgatagtt tgacattgca
     3181 cacggacttg taccagatca acatgatgca ggtttacttt gaccaaggga ttcacaataa
     3241 gaaggcggtc tttgaggtgt atttccgcca acagcctttt aagaacggct atgcggtttt
     3301 tgcaggtttg gaaagaattg tgaactatct tgaagacttg cgtttttcag atagtgatat
     3361 agcctatttg gagtcgcttg gttatcatgg ggcgttcttg gattaccttc gcaatttcaa
     3421 gttggagttg accgttcgtt ctgcccaaga aggggatttg gtttttgcta atgaaccgat
     3481 tgtgcaggtg gaaggacctc tagcccaatg tcagttggtc gaaacggctc ttttgaacat
     3541 cgtcaactac cagactttgg tggcgacgaa ggcagctcgt attcgttcgg ttatcgaaga
     3601 tgaacccttg atggagtttg ggacacgtcg ggctcaagaa atggatgcgg ccatctgggg
     3661 aacacgcgca gctgtgattg gtggcgccaa tggaaccagc aacgtgcgtg cgggtaagct
     3721 ctttgacatt cctgttttgg gaacccatgc ccatgccttg gtacaggttt atggcaatga
     3781 ctatgaagct ttcaaggctt acgctgcgac ccacaaaaat tgtgtctttc ttgtggatac
     3841 ctatgacacc cttcgcatcg gtgtaccagc tgccattcag gtggcgcgtg aactgggtga
     3901 tcagattaac tttatgggtg tgcggattga ctctggggat attgcttaca tttctaagaa
     3961 agtccgtcag caactggacg aggctggatt tacagaggct aagatttatg cttctaatga
     4021 tttggacgaa aatactatcc tcaacctcaa gatgcaaaag gccaagattg atgtctgggg
     4081 cgtgggtacc aagctgatta cagcttatga ccagccagct cttggtgcag tttacaagat
     4141 tgttgcaatc gaagatgaaa ctggtcagat gcgcaatacg attaagctgt ctaataatgc
     4201 tgaaaaagtt tctacgccag gtaagaagca ggtgtggcgc attaccagtc gtgaaaaagg
     4261 caagtcagaa ggcgactata tcacttatga tggtgtggat attagcgaca tgacagaaat
     4321 caagatgttc catccgacct atacatacat caagaagacg gttcgtaatt ttgatgccgt
     4381 tcctctcttg gtggatatct tcaaagaagg aatattagtt tacaacttgc ctagtttgac
     4441 tgacattcag gattatgccc gtaaggaatt tgacaagttg tgggatgagt ataagcgtgt
     4501 gctcaatccg cagcactatc cagtggattt ggcgcgtgat gtatggcaag ataagatgga
     4561 cttgattgat aagatgcgca aggaagccct tggtgaagga gaagaagaat gagtttgcaa
     4621 gaaacgatta tccaagagct gggtgtcaaa ccagtgattg atgcccagga agaaatccgt
     4681 cgttctattg atttcttaaa aagatatctg aaaaaacatc ccttcctaaa aacctttgta
     4741 ctagggattt ctgggggaca agactcaacc ttggcagggc gtttggcgca attagctatg
     4801 gaagaactgc gagctgaaac gggagacgac agctacaaat ttatcgctgt ccgcctgcca
     4861 tacggagtgc aagctgatga agcagatgct caaaaagccc tagccttcat ccagccagat
     4921 gtcagcttgg ttgtgaatat caaggaatca gctgatgcca tgacagctgc agttgaagcg
     4981 acaggtagtc ctgtttcaga cttcaacaag gggaatatca aggcacgttg ccgtatgatt
     5041 gctcagtatg cccttgctgg ttcccatagc ggagcggtca ttggaacaga ccacgccgcg
     5101 gaaaatatca caggtttctt taccaagttt ggtgacggcg gtgcggatat tctccctctt
     5161 taccgtctca ataaacgcca aggaaaacag ctcttgcaga aacttggcgc agagccagcc
     5221 ctttatgaaa aaatcccaac ggcagaccta gaagaagaca aaccaggcct agctgacgaa
     5281 gtcgcacttg gagtcaccta tgcagagatt gacgattacc tagaaggcaa aacaatcagc
     5341 ccagaagctc aagcgaccat tgaaaactgg tggcacaaag gccaacacaa acgacactta
     5401 cccatcaccg tatttgatga cttttgggag taaaaacctc cattggaggt ttttaccctg
     5461 agcctagaaa tgagaaagcg aagaaggtcc gggggacctt tttagcttct tgccctgaaa
     5521 ttaaaaagca agaaaaacct ccactggagg ttttcagcct ctcatcttga aataagaaaa
     5581 tgaggcagat tcggtaactc gaagagttcg atttcatcgt cttacccctg caacgatgac
     5641 taggtttgaa aaagattgct agagcgcatt tcaaaccagg cagcgactgc gtcaagaaat
     5701 tagaagacaa actcgttttc tagctgttac tgagttgagc ctttttacta cgagtataga
     5761 aataaggaag tgaggtagca tcatgaaatc tatcggtacg caaatattac agacagaacg
     5821 tttgatttta agaagatttg tggagagtga tgcagaagcc atgtttcaaa attgggcttc
     5881 atccgctgag aatctgacct atgttacctg ggatccccat cctgatgtcg aaatcactcg
     5941 aaactcgatt tgcaattggg ttgcttccta tactaatctc aactattata aatgggccat
     6001 ttgtctaaaa gaagacccag agcaagtgat aggagatatc agcattgtta agatagacga
     6061 ggctgattta agctgtgaaa ttggctatgt gttaggcaag gcttactggg gaaatggtat
     6121 gatgacagag actttgaaag ctatcttgga cttttgtttt actcaagcag gttttcaaaa
     6181 ggtcagagca cgttatgcca gtctcaaccc agcttcaggt cgtgtcatgg aaaaggctgg
     6241 aatgtcctat ctacaaacca ttgttaatgg tgtagagaga aaaggctatc ttgcggatct
     6301 tatttattat ggtataagta gggaagaatg ttgaattcta ttttctgttt ctatcgaagt
     6361 caactattta ttgtaaatat aataattagc attccaagtt tatttgaaac tttaaaatag
     6421 catattgatt agtacaagac agatgttcta gttccttctt taatctggtt tagtattagt
     6481 taaaaaatcg ctttaagctt gtaactaaga gggagctaat cgactagatt ctccagccga
     6541 acaggtggta atgtactttt tatagtgtaa tcctagctgt tgttaaattt aaaatagaat
     6601 cctctatcga gttagggaat taaattcaac caattttatt catgtttttt ctatcaaatt
     6661 atctaatatt aaaatagtct cattctgatg agaaaactat tctatttata ctgcatagtt
     6721 gtataaggaa tgatgaaaga tgagtgaaaa ttatcaagtt ggaatgtttg tatctaaata
     6781 tattagcatg tatttagata agatgtctgc aatcctttat atatgagatt gattacgata
     6841 tagttaaagt gttagttcaa taatcaatat atcattctaa gtctatttta tatataaagt
     6901 atattgttta acgtttgata gaaaagatgc tatactagaa gtaggatgta atggcaaaat
     6961 ctaataccaa taagcgctat gcattacgta aactcggaaa gatttttggt tctgcggtaa
     7021 tcgggctaac tttattttta ggaggaatct gaatacaagt tttgttcatg ctgctgatgg
     7081 gattcaatat gtcagagatg atactagaga taaagaggag ggaatagagt atgatgacgc
     7141 tgacaatggg gatattattg taaaagtagc gactaaacct aaggtagtaa ccaagaaaat
     7201 ttcaagtacg cgaattcgtt atgaaaaaga tgaaacaaaa gaccgtagtg aaaatcctgt
     7261 tacaattgat ggagaggatg gctatgtaac tacgacaagg acctacgatg ttaatccaga
     7321 gactggtcat gttaccgaaa aggttactgt tgatagaaaa gaagccacgg atacagttat
     7381 caaagttcca gctaaaagca aggttgaaga agttcttgtt ccatttgcta ctaaatatga
     7441 agcagacaat gacctttctg caggacagga gcaagagatt actctaggaa agaatgggaa
     7501 aacagttaca acgataactt atgatgtaga tggaaagagt ggacaagtaa ctgagagtac
     7561 tttaagtcaa aaaaaagact ctcaaacaag agttgttaaa aaaagaacca agccccaagt
     7621 tcttgtccaa gaaattccaa tcgaaacaga atatctcgat ggcccaactc ttgataaaag
     7681 tcaagaagta gaagaagtag gagaaattgg taaattactc ttactacaat ctatactgta
     7741 gatgaacgtg atggaacaat tgaagaaact acttctcgtc aaattactaa agagatggta
     7801 aaaagacgta taaggagagg gacgagagaa cctgaaaaag ttgttgttcc tgagcaatca
     7861 tctattcctt cgtatcctgt atctgttaca tctaaccaag gaacagatgt agcagtagaa
     7921 ccagctaaag cagttgctcc aacaacaggc tggaaacaag aaaatggtat gtggtatttt
     7981 tataatactg atggttccat ggcaacaggt tgggtacaag ttaatggttc atggtactac
     8041 ctcaacagca acggttctat gaaagtcaat caatggttcc aagttggtgg taaatggtat
     8101 tatgtaaata catcgggtga gttagcggtc aatacaagta tagatggcta tagagtcaat
     8161 gataatggtg aatgggtgcg ttaacagatt cctccctgcg acaaaattga taatagtatg
     8221 tctgaatata taattatata taaaaagcta tagataaggt catcttatag cgactataaa
     8281 aaagtgaaca agcctagtct tctgtacatt agaattcaga tttgttcact ttttgtttaa
     8341 tactcaatga aaatcaaaga gcaaactaga aaactagtcg caggttgctc aaagtactgc
     8401 tttgaggttg tagatagaac tgacgaagtc agtcacatat ataatccaag gtgacgttga
     8461 cgcggtttga agagattttt gaagagtata agtctttgat tttgagacta aggagagtca
     8521 gggtcaaagt agtgaatgat tctgaaataa ctcataatag tctcttgaga ggacagataa
     8581 gagtaacaaa tatggtgcta ctcatgtgtc ttgcattttg atttacaaac agcgaagaaa
     8641 gtataggaag ctgaaataaa atatagatga atcaatttgg agagtcaacg caatctatgg
     8701 caatattttc gaagctaggg ggaagcattt taatttcaat gtactaggcc tcttctacag
     8761 ctagctagag tttcaattgt agatgttctt tgaaataata tcttagcgat ttattggtat
     8821 tagaagattt agagaaattc tttctcaata aataaacatg gcgtcaccaa aactgaagaa
     8881 gcggtagtgt tcttggatgg aatggtggta ggcatctaag actaattcac ggcctgcaaa
     8941 ggcagaaacc aacatgacca gagttgattt tggcaggtgg aagttggttg agaaggcatc
     9001 cacgaccttc cactcatacc caggtttgat aaagatattg gtccaaccag aatctgcttg
     9061 gatttgccca tcaaacttgg aaccaatagt ttccaaggtg cggatagaag tggttccgac
     9121 agcgatgaca cgaccaccat tttttttgac agagcgaagg gtggcagcag cttcctcaga
     9181 aagttgatag aactctgagt gcatttcgtg ttcgtccaga ttatccacag aaacaggtct
     9241 aaaggttccg agtccgacat ggagagtcag atagactaga tgaacaccct tagcttggat
     9301 ttctgccagc agttctttgg tgaagtgaag accagcagtc ggtgctgcag cagagccact
     9361 ttccttggcg tagacggttt gataacgttc acggtcatct aatttttcgt ggatataagg
     9421 tggcagaggc atttctccca gactttccaa gacttctagg aaaattcctt ggtattcaaa
     9481 gtggacaatg cgtcccccgt gggtcaattc ttctgtaacg acagcgctga ggcggccatc
     9541 accaaagctg atacgagtac cgaccttgag gcgtttggca ggtttagcca gaacttccca
     9601 ctcgtctcca ctagtgttct taaggaggag aagttccaca tgacctcctg tctccacttt
     9661 ttgaccatag aggcgggcag ggagaactcg ggtgtcgttc atgacaaggg catcaccagg
     9721 ttccagcata tcaataatag agtggaaatg tttatcttgc atttctcctg tctcacggtt
     9781 gacgatgagg agtttggagg catcacgttt ttcaaggggc gtttgggcaa tcaattcctc
     9841 tggcaagtgg aaatcaaaat cagctgtatt catatatttg ttcattcttt ctaagttcta
     9901 atcctatcca ttataacacg tttcaagttt ggacgctctt tttttagaaa ttaaatcgtt
     9961 ttcacttgac aaaaattggt ctataccata taataaatat agacaaaaat ggaggatgaa
    10021 aagatgaaag ttattaaagt tgaaaaccaa gttcaaggtg gaaaagttgc ttttgagatt
    10081 ttgaaggaaa aattggctaa tggagctcaa actctaggac ttgcgacagg aagtagccca
    10141 cttgaatttt acaaggaaat tgttgagagt gaccttgatt tttcaaatct aaccagtgtc
    10201 aaccttgatg agtatgtggg ccttgatggg gacaatccac agtcttatcg ttacttcatg
    10261 caagaaaact tgttcaacca aaaaccattt aaagaaagtt tcttgcctcg tggtgttaag
    10321 gacaatgctg aagctgaagt tgaacgctac aaccaaattt tggctgacca tccagttgac
    10381 ctccaaatct tgggaatcgg tcgcaatggg catatcggct ttaatgagcc tggcactcca
    10441 tttgacagtc aaacgcatct agtagaactt gaccagtcta ctatcgaagc caatgcacgc
    10501 ttctttgcca agattgaaga cgtcccaacc caagctattt caatggggat taaaaacatt
    10561 ttggatgcca agtctattat tctttttgct tacggtgagt cgaaagcaga agccattgcc
    10621 ggaacagtat ctggcccagt gactgagaac ctacctgcaa gtagcctcca aaatcaccct
    10681 gatgtaacca tcatcgcaga tgctgaagcg cttagcttac tctaaaaata aaaaagcaat
    10741 gttctattga acgctttcaa ctcttgtata aatggaagaa aaaatcaaaa ttaaaccgca
    10801 tttttgcttg acaattattc cttttacgtg taaaatagaa tagatcttga acttgaaggg
    10861 agtgaaaaaa atgtctaaaa cagtagtacg taagaatgaa tctcttgacg acgcacttcg
    10921 ccgtttcaaa cgtgcggtta ctaaagctgg tactcttcaa gaaacacgca aacgtgaatt
    10981 ctatgaaaaa ccttctgtaa aacgtaaacg taaatcagaa gtagctcgta aacgtaaaaa
    11041 attctaattg aaatgaaagg ctagagaaat ctagtccttt ttcttttaaa taaatactcc
    11101 aaagcctgca aaaatctgaa acttcctcct acaatttgat ataatagaga gaagaactca
    11161 tttgaaggag gaaatgatgt cggttttagt aaaagaagtg attgaaaagc ttagactaga
    11221 cattgtctat ggtgaaccag aattgcttga aaaggaaatc aatacagcgg atattacgcg
    11281 acctggtctt gaaatgacag gctattttga ctactataca ccagagcgga ttcaactttt
    11341 ggggatgaag gagtggtctt atctgatcag catgccttcc aacagccgtt atgaagtttt
    11401 gaaaaaaatg tttctacctg agacaccagc agtcattgtt gcccgtggtt tggtggttcc
    11461 agaggagatg ttaaaggctg ctagagaatg taagattgct attttaacca gccgtgcagc
    11521 taccagtcgt ttatctggag agttatctag ctatctggat tctcgtttgg cagaacgtac
    11581 cagtgtgcac ggtgtcttga tggatattta tgggatgggc gtcttgattc agggagatag
    11641 tggaattggt aagagcgaga caggtcttga gcttgtcaaa cgtggtcacc gtttggtagc
    11701 cgatgaccgt gtcgatatct ttgccaagga tgagattact ctctggggtg aaccagctga
    11761 aattttgaaa cacttgattg aaattcgtgg ggttggtatt atcgatgtta tgagtctcta
    11821 cggtgcgagt gctgtcaagg attcttcaca ggttcagctt gctgtctatt tggaaaatta
    11881 cgatacgcat aagacctttg atcgtcttgg aaataatgca gaggaacttg aagtttctgg
    11941 cgtagccatt cctcgtattc gtattccagt taaaacaggt cgtaatatct ctgttgtgat
    12001 tgaggcagct gccatgaatt atcgtgccaa ggaaatgggc tttgatgcga cccgtttgtt
    12061 cgacgaacga ctgacaagtc taatcgctcg aaatgaggtg caaaatgctt gatccaattg
    12121 ctattcaact aggaccccta gccattcgtt ggtatgcctt atgtattgtg acaggcttga
    12181 ttcttgcggt ttatttgacc atgaaagaag cacctagaaa gaagatcata ccagacgata
    12241 ttttagattt tatcttagta gcctttccct tggctatttt aggagctcgt ctctactatg
    12301 ttattttccg atttgattac tatagtcaga atttaggaga gatttttgcc atttggaatg
    12361 gtggtttggc catttacggt ggtttgataa ctggggctct tgtgctctat atctttgctg
    12421 accgtaaact catcaatact tgggattttc tagatattgc ggcgcctagc gttatgattg
    12481 ctcaaagttt ggggcgttgg ggtaatttct ttaaccaaga agcttatggt gcaacagtgg
    12541 ataatctgga ttatctacct ggctttatcc gtgaccagat gtatattgag gggagctacc
    12601 gtcaaccgac tttcctttat gagtctctat ggaatctgct tggctttgcc ttgattctga
    12661 tttttagacg gaaatggaag agtctcagac gaggtcatat cacggccttt tacttgattt
    12721 ggtatggttt cggtcgtatg gttatcgaag gtatgcgaac agatagtctc atgttcttcg
    12781 gctttcgagt gtcccaatgg ctgtcagttg tccttatcgg tctcggtata atgatcgtta
    12841 tttatcaaaa tcgaaagaag gccccttact atattacaga ggaggaaaac taaatgttag
    12901 aagttgcata tattcttgtt gccctagctt tgattgtctt tttggtctat ctgatcatta
    12961 ctgtacaaaa gcttggtcgt gtcatcgatg aaacagaaaa gacgattaaa accttgactt
    13021 cagatgtgga tgtgaccttg catcacacca atgagttgtt ggctaaggtc aatgtcttgg
    13081 cagatgatat caatgtcaag gtggctacga ttgatccact cttcagtgct gttgcagatt
    13141 tatctctatc tgtttcagac ctcaatgacc atgcgcgtgt cttgagcaag aaagcttcat
    13201 cagctggttc aaaaacactc aagactggtg caagtctgtc agctcttcgt cttgcaagta
    13261 aatttttcaa aaaataaaaa aggagaatcc ttatgggtaa attatcctca atccttttag
    13321 gaaccgtttc aggtgcagct cttgccttgt ttttaacaag tgataagggc aaacaagttt
    13381 gcagtcaggc tcaagatttt ctagatgatt tgagagaaga tccggagtat gccaaggagc
    13441 aagtctgtga aaaactgaca gaagttaagg agcaggctac agattttgtt ctgaaaacaa
    13501 aagaacaggt tgagtcaggt gaaatcactg tggacagtat acttgctcaa gctaaatcct
    13561 atgcttttca agcgacagaa gcatcaaaaa atcaattaaa taatctcaag gagcaatggc
    13621 aagaaaaagc cgaagctctt gatgactcag aagagattgt gattgatata acagaagaat
    13681 aaaccatcac catctccgga cggactatgt atctggggat ggtgattttt atctggaatc
    13741 tagtctttgt ggtataataa ttactatgca gaaaaaacca acgtcagcct atgtgcacat
    13801 cccattttgt acccagattt gttattattg tgatttttca aaggtcttca tcaaaaatca
    13861 gccagtcgac agctatttag agcatctgct ggaagagttt cgttcttatg atattgaaaa
    13921 gttgtcaacc ctttatatcg gtggtggaac accgacagcc ctgtcggctc cgcaactgga
    13981 ggtgttactg aatggcttga ctaaaaactt ggatttgtct gtcttggaag agttgaccat
    14041 tgaagccaat ccaggcgatt tggatgcgga taagatagct gttttgaaaa attcggctgt
    14101 caatcgtgtt tcgctaggtg tccagacctt tgatgataag atgttgaaaa agattgggcg
    14161 cagtcatttg gagaaggata tttatgaaaa tatcgatcgc ctgaaactgg ctggttttga
    14221 caatatctcc attgatttga tttatgctct gcctggtcag accatggagc aagtaaagga
    14281 aaatgtggct aaagccattg gattggatat tccccacatg agtttgtata gtttgatttt
    14341 agaaaaccat acggtcttta tgaaccggat gcgacgtggg aaattgcctc tgcctaagga
    14401 ggaactagaa gcggagatgt ttgagtacat cattgcagag ctggagcgag cgggttttga
    14461 gcattatgag atttctaatt tctccaaacc cggttttgaa agtcgtcata atctcatgta
    14521 ctgggacaat gctgaatact atggtattgg tgcaggggca tctggttatg tcaatggagt
    14581 acgctataaa aatcatggtc ccattcgtca ttatctcagt gcggttgagg aaggcaatgc
    14641 ttgtattaca gaagatcacc tgagtcaaaa ggagcaaatg gaagaagaaa tgttcttggg
    14701 actccgcaag aaatccgggg tttccatggc gcgatttgag gaaaaatttg gacagtcttt
    14761 tgctggactt tatggagaaa ttgtcagaga tttggttcaa caaggcctca tgcagattga
    14821 gggtgaccac gttcgcatga caaagagagg tctctttttg ggagatactg tagcagaacg
    14881 atttattttg gagtaggatc atgggcttaa cttatcaaat gaaaatgaaa attccttttg
    14941 atatggctga tatgaacggt catatcaaac ttccagatgt gattttgctg tcactgcaag
    15001 tttcagggat gcagtcgatt gaactgggag ttagtgataa ggccattttg gaagaatata
    15061 atctggtctg gattatcgta gaatatgata ttgaggtggt tcgtttgcct cgttttgcgg
    15121 aagaaattac catcgaaacg gaagccttga gctacaatcg acttttttgc taccgtcgct
    15181 ttactattta taatgaagcg gagcaggagc tcatccacat gatggcgacc tttgttctca
    15241 tggaccgcga cagtcgaaaa gtccatgttg ttgaacctga gattgtggct ccttaccagt
    15301 ctgattttga taaaaaactt atccgtggac cgaagtatga gtccttggaa gaaccgatca
    15361 gcaaggatta ccatgttcgt ttttacgact tggatatgaa tggtcatgtc aataacagta
    15421 aatacttgga ctggattttt gaggtcatgg gagcggattt tttgacccac tatattccca
    15481 agaaaatcaa cctcaagtat gtcaaggaag ttcgaccagg tggggtgatt acatcggctg
    15541 ttgaacggac tggactggaa agcaagcatg aaattacaag tgatggggtt atcaatgccc
    15601 aagcaatcat tacttggcaa gaaataaaaa aggcttaggg agaaatagat gaaatataaa
    15661 ggctatttaa ttgatttaga cggaaccatt tataagggga aagaccgaat ccctgctgga
    15721 gagacttttg ttcatgaatt gcaaaagcgg gacattccct atctctttgt aaccaacaat
    15781 acaacccgca ctccagagag tgttaaggag atgttggctc agaattttaa tatcgatacg
    15841 cccctatcga ctgtctacac agcgactttg gcaaccatcg actatatgaa tgatttgggg
    15901 cttgaaaaga ccgtctatgt cgtcggagaa gcaggactca aggaagccat caaggcggct
    15961 ggttatgtgg aagacaagga aaaacctgcc tacgtggtag ttggtctgga ttggcaagtc
    16021 gactatgaaa aatttgccac agcaactctt gctattcaaa agggtgccca ttttatcgga
    16081 accaatcctg acctcaacat cccgacggaa cgcggtcttt tgccaggtgc tggctcactg
    16141 attacgctgc ttgaagtagc gacacgagtg aagccggttt atatcggaaa accaaatgcc
    16201 atcattatgg acaaggcggt tgagcactta ggtttggaac gtgaagagtt aatcatggtt
    16261 ggggacaatt atttgactga tattcgggct gggattgaca atggcattcc aacgctcttg
    16321 gtgacgacag gttttactaa ggcagaagaa gtagctggcc tgccaatcgc accgacacat
    16381 gtggtttcta gccttgcgga gtgggatttt gatgaaaact aaattgatct tttggggctc
    16441 tatgctcttt ctcctctccc tctccatcct tctgaccatt tatctggctt ggattttcta
    16501 tcctatggag attcagtggc taaacttaac gaatcgagtc tatctaaaac cagaaaccat
    16561 tcaatacaat tttcatatct tgatgaatta tctgaccaat ccttttagtc aggtcttaca
    16621 gatgcctgat tttcgttcgt cagcagctgg tctgcaccat ttcgcagtgg tcaagaatct
    16681 ctttcatttg gttcagctag tagctctagt gacactgcca agtttctatg tctttgtcaa
    16741 taggattgtg aaaaaggact ttttgtctct ttatcgaaaa agtctcctgg ctctagtagt
    16801 cttacctgtg atgattggac ttgggggagt tttgattggt tttgaccaat tctttactct
    16861 tttccatcaa attctctttg tgggagatga tacctggctt tttgatccag ccaaggatcc
    16921 tgttattatg attttgccag agaccttctt tcttcatgcc ttcctcctct tttttgccct
    16981 ctatgaaaac ttctttggct atctgtatct gaaaagtcgt aggaagtgaa aggaaagatg
    17041 tttttaattt acatatataa aaatatatat cgtttaaaac catcgttaag agtgaattaa
    17101 ccaaatccaa ttgtgtcaat cgttcgcaaa aagcctattt ttcttgaaaa aataggcttt
    17161 ttttctaaaa atccctagtc aagcgcttta tttttttgta taatagaagt agcaaagtat
    17221 agataagaag agaaaagcat gattacacta tttctatcac cgagctgtac atcatgtcgt
    17281 aaggctaagg cctggttaga aaaacataag gttccctttg tggaacacaa tattacgacc
    17341 agtcctttaa caagaaaaga attgcaacac atcctttcct tgaccgaaaa tggtactgat
    17401 gacatcattt caactcgttc aaaaattttt caaaaattga atattgatgt agagagtatt
    17461 tcggtatcgg aattgcttca tttgattgag cagtatccta gtcttttgcg tcgtccaatt
    17521 attattgatg ccaagcgtat gcaaatcggt tttaatgaag atgagattcg tgcttttctc
    17581 cctcgtagtt atcgtaagca agaactaaaa gaagcaagaa tgagagctgg tattagttaa
    17641 tgaacaaaca gtatagttac ccactagatt tgtcgtggag cactgaagaa cttgcttcag
    17701 tgctttcttt ttttaatgat gttgaagctg cctatgaagg caaagtagag gctaaaaagt
    17761 tactggactc ctataaggga ttcaaagcgg tcgtgccaag caagagcgaa gagaaacgtt
    17821 tgggacgaga atttgaaacg gttagtggct attcacttta ccgagcagtt caggctgcca
    17881 aagaaaaagg ggaaggaaag atttctcttg gaaagtaaat ttgaatttgc taaagagctt
    17941 gttaagaaag caggtcagta cattcttgac catatgcagg aagacttgcg tgttgaaacc
    18001 aagtcctctc caacagattt ggtgaccaga ctggacaaag aagttcagga actcttggtt
    18061 ggtgagattt tgtcccgtta tcctgaggat aagatttgtg ctgaagaagg ttgtctgcga
    18121 gcctcggttc aagagggcaa ggtttgggtc attgatccca ttgatggtac caataatttt
    18181 gtagcccagc aggaagattt tgctgttatg atggcttatt ttgaaaatgg tcagggacag
    18241 tttggtctga tttatgatgt ggtcaaaggg gattgttacc acggtggtgg ggaatttcca
    18301 gtttgtctaa atgataggtc cctagctcct tttaaaacta aaccacttgg agattttctc
    18361 attgcaggga atagtggtat gctggaaacc aatgaatggg ggctggctga tttgagccga
    18421 gcggttcttg gtgttcgtgt ctatggaagt gcggccatta gttttgccaa gattttgtca
    18481 gggcgtttgt tgacctatct cacttatctg caaccatggg attatgccgc agctagtatt
    18541 ttaggggaaa gtctgggcta tcgggttgtg accctttttg gtgaagctcc tgattttcaa
    18601 accagacagc cggtcatgat ggtgcctctt gagatgcagg aggaaattca gtcctatatt
    18661 tacgaaagga aaagaactta aatgcaattt ccagaaggat ttgttgaaaa atataaagag
    18721 atactaggag atgaggcaag agattttctt gcctcttttg aggaggaagc ggtttcggcc
    18781 tttcgggtca atcccttaaa agaagagcaa ctttcctttt ctgatgccat tactcaaacc
    18841 ccttggggcc actatgggaa ggtttcaggg aaatcgcctg agcatgctac aggtttagtt
    18901 tattcgcaag aacctgctgc tcaaatggtg gctcaagtag cccaacccag tcctggtatg
    18961 aaggtcttgg acttggctgc tgcaccgggg ggcaaatcaa ctcaactggc agcctatcta
    19021 gcaggggagg ggctacttgt ttccaatgaa atttcaagca aacgggctaa gattttggtt
    19081 gaaaacatgg agcgttttgg agcgacaaat gtcgtggtga ccaatgaatc tgccgaccgc
    19141 ttggtcaagg tctttaaagg ctattttgac ttaatcgttc ttgatgcccc ttgctctggt
    19201 gaaggaatgt ttcgtaagca acctgatgct atggactatt ggagcttaga ttatccgagt
    19261 caatgtgcta gcttgcaaag agaaattctg gaggatgcag tgaccatgtt agctgaaggt
    19321 ggtcatctgg tttattcgac ctgtacctgg gcacccgagg aaaacgaaga gattgtcaat
    19381 tggttgctgg aagagtatga ttttgacttg ttgccagtag agcatatcaa tggaatggta
    19441 gctggtattg acctgccaga aacggctcgg atgtatcctc atcagtttaa gggagagggt
    19501 cagtttgttg cccatctaca gtttaagggt aacaatccag ctcctaaatt taaggcaagt
    19561 aagagtaacc tcagccgtga acaagttgcc ttgtggcagg aatttgccca aaaccatttg
    19621 aaggtcaatc taccgggtat cttgcagact tttggagacc agctttatct cttgccagaa
    19681 cttttgccag atttagggaa gctcaagatt gctcgcaatg gactgcatct gggcactttt
    19741 aagaagaaac gctttgagcc cagtttcgct cttggtttag ccttgaaacc gagtcaggtc
    19801 gaacagtcgg ttgaaattgg ccaagaagcg tttgtcaagt atgcggctgg agaaatcgtt
    19861 cagctggcag aaagtctgcc aaatggttgg taccaagttt tggttaaagg caatggcttg
    19921 ggctttgcaa aggttactgg aaatgttttg aaaaattatt ttccaaaagg cctcagattc
    19981 aaatgaaaat gctagtcaaa tatcttattc tatgttatag tgaaagtatg gattttttta
    20041 gtaggatgat tcttgatatc ttgattgcca ttacatgtag ttttactact caatgaaaat
    20101 caaagagcaa actaggaagc tagccataga tagctcaaaa cactggtttg aagagatttt
    20161 caaagagtac aagaagttta gttattagcg ttcttaccgc ttgtaaacta gatttctcat
    20221 aaaatagaat cttttccttt tagttgtaaa ctagtctggg agagtagaga ggtttgagat
    20281 acctttctag cttttggatt atcatctaag gagagtaatt tcccttgcat taaaaagggg
    20341 aaaaagagac acgaaatgac tataatgggt gacaatgggg gaagggatag acaagagatt
    20401 ttatccgcat atgaaaaaag gaggttagga aagagtaata tagcctatat tatataaata
    20461 atcaattgcg cagaaatttg gtaagaattc atgcgtcaac tcataaagaa ctacttaaaa
    20521 aattcacagt attcataatt attttcgagg agaaaaacag tgaaaaaaag aaaaaagctt
    20581 gctctgtctc ttatcgcttt ttggctgacg gcttgtttag taggctgtgc tagctggatt
    20641 gatcgtggag aatccataac ggctgttggc tcaactgcct tgcaaccctt ggttgaagta
    20701 gcggcagatg aatttggcac gatccatgtt ggaaaaacgg tcaatgtcca agggggaggt
    20761 tctggtacag gcttgtccca ggttcagtct ggggcagttg atataggaaa ctcagatgta
    20821 tttgctgagg aaaaagacgg aattgatgct tctgctcttg ttgaccacaa ggtcgcggta
    20881 gctggcttgg ctctgattgt caataaggag gttgatgttg ataacctaac gacagagcaa
    20941 cttcgtcaaa tcttcatagg tgaggtaacc aattggaaag aggttggtgg taaggactta
    21001 cccatctctg ttatcaatcg ggcagccggc tctggctctc gtgctacctt tgatactgtc
    21061 attatggaag gtcagtctgc catgcaaagt caggagcagg attcaaatgg agcggtaaaa
    21121 tcaatcgtat caaaaagtcc aggagctatc tcttatttat ctcttaccta tatagatgat
    21181 tcggtcaaaa gcatgaagtt gaatggttat gacttaagtc cagaaaatat aagtagcaat
    21241 aattggccct tgtggtctta tgagcatatg tatacattgg ggcagcccaa tgagttagct
    21301 gcagaatttc tcaattttgt tctctcggat gagacccaag aagggattgt caaaggattg
    21361 aagtatattc cgattaagga aatgaaggtt gaaaaagatg ctgccggaac tgtgacagtg
    21421 ttggaaggga gacaataatg aatcaagaag aattagctaa gaaaatgttg cttccatcaa
    21481 agaattctcg tctggagaaa ttaggaaaag gtttgacctt tgcctgtctt tctttgatag
    21541 tcatccttgt ggccatgatt ttggttttcg tagcgcaaaa aggcttgtcg accttctttg
    21601 tcaatggtgt gaatatcttt gactttcttt tgggaggaac ttggaatcct tctagtaaag
    21661 aatttggtgc ccttcctatg attttgggtt cctttatcgt taccattctc tcagccctta
    21721 tcgcaacccc ctttgctatt ggtgcagcag tttttatgac cgaagtatca ccaaaagggg
    21781 cgaagatttt gcaaccagct attgaactcc tggttgggat tccttcagta gtgtatggat
    21841 ttattggctt gcaagtcgtc gttccctttg ttcgcagtgt ctttggtggg actggttttg
    21901 ggattttgtc agggatttcc gtcctctttg tcatgatttt gccgaccgta acctttatga
    21961 caacggatag cttgcgtgcg gttcctcgtt attatcgtga ggctagtttc gctatgggag
    22021 ccactcgctg gcagactatc tggcgtgtga tcttgaaggc ggcccgttct ggtattttca
    22081 ctgcagtggt ctttgggatg gcgcgtgcct ttggtgaggc tttagctatc cagatggttg
    22141 tcggaaactc agctgttatc ccaacttcct tgaccacacc agctgcaact ttaacttcta
    22201 tattaactat gggaattggg aacactgtca tgggaactgt aaataataat gttctctggt
    22261 cactggcctt ggtactgctc ttgatgagtt tagtttttaa cagtgtgatt aaattgatta
    22321 cgaaagaaag aggaaagaaa aattatgcgc gctaagaaat tagataaact tgcaacagct
    22381 gtcctctata cgattgctag catcattgtg acaatcttgg cttccttgat tctctatatc
    22441 ttggttcggg gcttgcccca tatctcttgg tctttcttga ctggaaggtc ttctgctttt
    22501 caagcaggtg gtgggattgg cattcagctt tacaattcct ttttcctatt ggtcattacc
    22561 ttgattattt ctgtacctct ttctatggga gctgggattt acttggctga atatgctaaa
    22621 aaaggtcctg ttaccaactt tgtgcggact tgtattgaaa ttttgtcctc tttaccatca
    22681 gtggtggtgg gtctctttgg ttacttgatc tttgtagtcc agtttgagta tggattttca
    22741 atcatttcag gtgccttggc cttgacagtc tttaacttgc ctcagatgac gcgtaatgta
    22801 gaggatagtt tgaaacacgt tcaccatacc caacgtgagg ctggtctggc tcttgggatt
    22861 tctcgctggg agacagtgat tcatgttgtt attccagaag cgcttccagg tattgtaacg
    22921 ggtgtcgtct tggcatctgg tcgtatcttt ggcgaagctg cagctctgat ctatacagca
    22981 gggcaatcgg cgccagctct tgactggtct aactggaata tcctcagtgt gactagcccc
    23041 atctctatct tccgtcaagc agaaaccttg gctgtccata tctggaaagt caatagtgaa
    23101 ggcactattc cagatggaac cattgtatca gcaggttctg ccgctgtgct cctgatcttt
    23161 atcctgattt ttaactttgg agctcgtaag ttcggaagct atctacacaa gaaattaacc
    23221 gctgcctaaa ggagaagcca tgtcaacata taactgggat gagaagcata tccttacctt
    23281 tcctgaagaa aaagtagccc tttctactaa ggatgtccat gtttactatg gtaaaaatga
    23341 atccattaag gggattgata tgcaatttga aagaaataaa attacagctt tgattggtcc
    23401 gtcgggatcg gggaaatcta cctacttacg cagtctcaat cgcatgaatg atactattga
    23461 tattgctaaa gtaactgggc agattctcta tcgtggaatt gatgtcaacc gtccagaaat
    23521 caacgtttat gaaatgcgta aacacattgg aatggttttt caacgcccca atccatttgc
    23581 taagtcaatt taccgtaata ttacctttgc gcatgaacgt gctggagtta aggataagca
    23641 agtcctagat gaaatcgtag aaacctccct tcgtcaggct gccctctggg atcaggttaa
    23701 agacgatctc cacaagtcag ccttgacctt atcaggtggt cagcaacaac gtctctgtat
    23761 cgctcgtgcc atctctgtta agccagatat cctcttaatg gatgagccag cctcagcctt
    23821 ggatccgatt gcgaccatgc aactagaaga gaccatgttt gagctcaaga aaaactttac
    23881 catcatcatt gtaacgcata atatgcagca ggctgctcgt gcaagtgact atacaggctt
    23941 cttttacttg ggtgatttga ttgagtatga caagactgca actattttcc aaaatgccaa
    24001 gctacagtcc accaatgact atgtatctgg tcactttggt tagaaaggaa accgtatgac
    24061 agatgcgatt ttacaggtat cagacctgtc cgtttattat aataaaaaga aggctttgaa
    24121 tagtgtttcc ctatctttcc aacctaagga aattacagcc ttgattggtc catctggatc
    24181 agggaagtca accctcctca agtctctcaa ccgcatggga gatctcaatc cagaggtgac
    24241 cacaactgga tccgtggtgt acaatggtca caacatctac agtccgcgta cagatacggt
    24301 tgaattacgt aaggaaatcg gaatggtttt ccaacaacct aatcctttcc ctatgactat
    24361 ctatgagaat gttgtctacg ggcttcgtat caatggaatt aaggataagc aggttctgga
    24421 tgaagccgta gaaaaagcct tgcaaggtgc ctctatctgg gatgaggtca aggatcgtct
    24481 atatgattca gctattggat tgtcaggtgg tcaacagcag cgtgtctgcg tggcccgtgt
    24541 cttggcaact agtcctaaaa tcatcctctt ggatgagcca acttcggctt tggatccgat
    24601 ttcagctggt aaaattgagg aaaccttgta tggtctaaaa gacaagtaca ccatgcttct
    24661 ggtaacccgt tccatgcagc aagcttcacg tatctctgat aagacaggat ttttcctaga
    24721 tggagatttg attgaattta atgataccaa gcagatgttc cttgatcccc aacacaagga
    24781 aacggaagac tatattacag gaaaatttgg ataaggagat gaaagatgtt acgatctcaa
    24841 tttgaagaag atttagagaa attacataac cagttctacg ctatgggaca agaagtgctc
    24901 tcacaaatca atcgtacggt acgtgctttt gtcacgcatg accgtgacct ggcaaaagag
    24961 gtcatcgaag atgatgcaga agtaaatgaa tacgaagtga aactggaaaa gaaatcattt
    25021 gaaatgatcg cactccaaca accagtctct caagatttgc gtacagtctt gactgtcctt
    25081 aaggctgtat cagatgtgga gcgtatgggg gatcacgctg tagccattgc ccaggcaacc
    25141 atccgtatga agggggaaga gcgcattcca gctgtagagg aagaaattaa aaaaatggga
    25201 cgtgaagtta aaagcgttgt tgaagcagca cttgatcttt atcttaatgg ttctgttgac
    25261 gacgcatacc gggtggcctc catggatgag caaattaacc actattttga aactatccgt
    25321 gaccttgcga ctgaagagat taagaaaaat ccagaagcca ttgttacggg tcgtgattat
    25381 ttccaagtta tttcctactt ggagcgtatc ggtgactatg ctaaaaatat ctgtgaatgg
    25441 gttgtctact ttgaaacagg taagattgtc gaactataaa atagtttaat tctgtataaa
    25501 aaggagcttg aaatcaactg atggctcctt tttttgaacg aaaaaatatt tttgagataa
    25561 aaatttaaaa aacacttgac aagtaacttg gatatcgtta taattataca aaattaacag
    25621 agaggttgtt tatttatgaa atcaaaaaaa tggatatttg ttttatgtaa ttttcttgca
    25681 agtttcttct tagtagcttg ccagtcaggt tctaatggtt ctcagtcagc tgttgaggcc
    25741 attaagcaaa aggggaaatt agttgtggca accagtcctg actatgcacc ctttgaattt
    25801 caatcattgg ttgatggaaa gaaccaggta gtcggtgcag acatcgacat ggctcaggct
    25861 atcgctgatg aacttggggt taagttggaa atctcaagca tgagttttga caatgttttg
    25921 accagtcttc aaactggtaa ggctgaccta gcagttgcag gaattagtgc tactgacgag
    25981 agaaaagaag tctttgattt ttcaatccca tactatgaaa acaagattag tttcttggtt
    26041 cgtaaggctg atgtggaaaa atacaaggat ttaactagcc tagaaagtgc taatattgca
    26101 gcccaaaaag ggactgttcc agaatcaatg gtcaaggaac aattgccaaa agctcaatta
    26161 acttccctaa ctaatatggg tgaagcagtc aatgaattgc aggttggaaa agtagatgct
    26221 gttcatatgg atgagcctgt tgcacttagt tatgctgcta aaaacgctgg cttagctgtc
    26281 gcaactgtca gcttgaagat gaaggacggc gacgccaatg ccgttgctct tagaaaaaat
    26341 agtgatgatt tgaaagaagt ggtagacaag gtcatccaaa aacttaagga tgaaggaact
    26401 taccaaagct atcttgaaaa agcagcaagt ctaactgaag ttgaagaata agaaaaagca
    26461 gagttggatg tcattccaat tctgctttta aatagtttaa aacattttcc agattgtcta
    26521 gggacacctt ggcaaattga taaggacagg tgcttaaata ggcctcttca aagaagtcca
    26581 agcccagttg actgtgtttg agactggcga ttttaggata ctgtctcagg tctagcaata
    26641 aaccatcgtg taggggataa tgaggaaggg gacaaggtgt tttagttatc ctttcctcga
    26701 tttgcttttg gtaagattca tgattggtca agcgagccaa acgatttttt tcaaacaatt
    26761 gactgtcaat atagagtgac agggcctttt gcataatgag gttgctgtcg tagtctagga
    26821 tatttttgta ggccacaaat gcttctttga tagcatttgg aagaatgagt gctgtaatac
    26881 gaagggcagg aaaaaggctg gtcgagaagg acttgatata aatgacatgc tcctctgtat
    26941 caagatagtg gaaggtttgg cccttcttgg agtccaaatc acccagataa tcgtcctcta
    27001 cgatatagac atcatacttg gcagctaagt taagaataga tcgtttgtct tgctcagaat
    27061 aggaatgtcc cagaggatag tgaaatctag gaatggtgta gaaaaattta atttttcctg
    27121 ttttgaagtg gccttccagc tcctccaagt caatcccatc aatgcctcgt tcaatcgttt
    27181 gatagtccag cccctgtgca atcaagaggc gattcatccg atggtaggtt ggctgttcca
    27241 ccaagatttc cttggcttgt ctaggaaagg atatttgaga gaggataaac aaggcttgtt
    27301 gggttccaga agtcagtact agttggttag ccttgcagta gagagcttgc tcaaatagga
    27361 gtttgtgaat ggactgtctt aggtcttcta atccttcttg attgtcatag tagttgaaga
    27421 ggtagttttc tcgtccaatc aaggtttcat tgacacagag tcggaaatcg tcataggcac
    27481 tggcatgttc gtcggtaacc tcgatttcta ggtcttgatg ttgcccttgt tctaatacat
    27541 agtagccact ctgaggcttg gcatagaggt attgttcgtg ccgtaattcc agcagggctc
    27601 gttgaatggt gtccttgctg cagtgaaagt caaggctcag ttgacggata gaaggcaggc
    27661 gactacccgt cggaaatcgt tcagactcga taccattttt cagataggaa acgacctctt
    27721 tgtacttgct ttgtttcttc attccagacc tcccttgatt ttgttacatt gtaccctttt
    27781 ttttccttct tggcaatgtc tagagcgttt ctctcgttca catctagggg caaatgtggt
    27841 atacttagga gtaagattta tagaataaca gacaagaaag tgaatggata agaacgtgca
    27901 ggaaccagtg aaattatttc aatacaatac tcttggagcc ttgatggctg gcctttatgg
    27961 tggtactatg acagtaggag aattgctaga acatggtgac cttggtttgg gaaccttgga
    28021 ttccattgat ggggaattga ttgtcttgga tggcaaggct tatcaggcca aagggtcagg
    28081 agaccagcca gaaattgtgg aagtgtcacc agatgccctt attccttacg ctgcagtggt
    28141 accgcatcag gcagaggtca ttttccgcca gcgctttgag atgacagaca aggaattgaa
    28201 agaacgaatt gagtcttatt atgatgggga aaatcttttc cgctctatca agattcgggg
    28261 ggaattttta catatgcatg tgcgtatgat tcctaagtca acacccgata ccaagtttgc
    28321 tgatgtcgca acccatcaac cggaatatag tcgtgacaat gttgcgggga ctattgttgg
    28381 tttctggacg cctgagattt tccatggggt cagtgtggca ggctaccatc tgcacttcat
    28441 atcagatgat ttgaccttcg gtggacatgt catggatttt gtcatcaagg aaggcattat
    28501 cgaggtggga gcagttgacc agttggacca acgtttccca gtccaagacc gtcaatactt
    28561 gtttgctaag ttcaatgttg acgagatgaa aaaagatatt gaaaaggcag aataggagaa
    28621 gaaaatgacc attcatatca ttattaccat gttgctgttg ctagcttttc tgataggaag
    28681 catttggttt gccaaaaaga aataccagat taatctagct gtcttgggct aaggtgcagt
    28741 cgctttcttt gtctcttcgc agattttgga aaaactggtg catatcttga ttttgcaccc
    28801 tcaaaaagac ggtagtattg ccctcttgca agatcatccg cttatctata ttatctatgg
    28861 cctagccatg gcagcatttt ttgaggaaac ggctcgtctt gttttcttca aatggttgga
    28921 gaaaaagaga agtttggaaa aggcagacgc cttggcctat ggactggggc atggtggctt
    28981 ggagttgatt tttctaggcc ttactagttt actcaatctt tacattgttc tttcggcagt
    29041 tcaaactcag aatccacagg ttatgcaatt actgtctgaa aatatgttaa aaaccattca
    29101 gtcactttcg gtctggcaga tttatttgct tggttttgag cgaatcttgg cgcttggttt
    29161 ccaattactt ttgacagttt gggtttacca agctgttcgc cagaagaaat ggatttatct
    29221 cctagcagcc tatggcttgc atgccttctt tgatctggca ccatctcttt tccaagtagg
    29281 ctggttgaca aatccagtct tggttgaagt gattctagca ctggagctcg ttctggtcgc
    29341 ctatggaacc aaggaaatct tttgtaaaaa atcataaaaa ggggggaacc tctttttctt
    29401 atgcaaaatc caaacaaggt atttttatgg tcgtcaaatg tctctaaaaa tggtataatg
    29461 gaatgaattt tgtaaaagga agagtgacat gtctgtaaga gaaaaaatgc ttgaaatctt
    29521 agaaggaatt gatatccgtt ttaaggaacc cttgcatagc tatagttata caaaagtagg
    29581 tggagaggct gattatttgg tctttccacg aaatcgtttt gagttggctc gcgttgtgaa
    29641 atttgccaac caagaaaata tcccttggat ggttcttggc aatgcaagca atatcatcgt
    29701 tcgtgatggt gggattcgtg gatttgtcat cttgtgtgac aagctcaata acgtttctgt
    29761 tgatggctat accattgaag cagaagctgg ggctaacttg attgaaacaa ctcgcattgc
    29821 cctccgtcat agtttaactg gctttgagtt tgcttgtggt attccaggaa gcgttggcgg
    29881 tgctgtcttt atgaatgcgg gtgcctatgg tggcgagatt gctcacatct tgcagtcttg
    29941 taaggtcttg accaaggatg gagaaatcga aaccctgtct gctaaagact tggcttttgg
    30001 ttaccgtcat tcagctattc aggagtctgg tgcagttgtc ttgtcagtta aatttgccct
    30061 agctccagga acccatcagg ttatcaagca ggaaatggac cgcttgacgc acctacgtga
    30121 actcaagcaa cctttggaat acccatcttg tggctcggtc tttaagcgtc cagtcgggca
    30181 ttttgcaggt cagttaattt cagaagctgg cttgaaaggc tatcgtatcg gtggcgtaga
    30241 agtgtcagaa aagcatgcag gatttatgat caatgtcgca gatggaacgg ccaaagacta
    30301 cgaggacttg atccaatcgg ttatcgaaaa agtcaaggaa cactcaggta ttacgcttga
    30361 aagagaagtc cggatcttgg gtgaaagcct atcggtagcg aagatgtatg caggtggttt
    30421 tactccctgc aagaggtagt ggggacctga cagagccccg atcggttaat ctatgaaaaa
    30481 gaaggaattt atgccaattg aaaaagccaa ttatcgaatt caaaaacgtc tctaaagttt
    30541 ttgaagacag caacaccaag gttctcaaag acatcaactt tgagttggaa gaagggaaat
    30601 tctacaccct tctaggtgca tctggttcgg ggaaatcaac tatcctaaac attattgcag
    30661 gtttactgga tgcgacgaca ggagatatca tgctagacgg tgttcgtatc aatgatattc
    30721 caaccaacaa gcgcgacgta cataccgtct tccaatccta tgccttgttc ccacatatga
    30781 atgtgtttga aaatgttgcc tttccacttc gcttgcgtaa aattgataag aaagaaatcg
    30841 agcagcgtgt agcggaagtt ctcaagatgg ttcagttgga aggttatgaa aaacgttcca
    30901 tccgcaaact ttctggagga caacgtcagc gtgtggccat cgcccgtgct atcatcaacc
    30961 aaccccgtgt ggtcttgttg gacgagcctt tatcagcgct ggacttgaaa ttgagaacag
    31021 acatgcagta cgaattgcgt gaattacaac aacgattggg cattaccttt gtctttgtca
    31081 ctcacgatca ggaagaagct cttgccatga gtgactggat tttcgttatg aatgatggcg
    31141 agattgtcca gtctggaacc cctgtggaca tctacgatga gccaatcaac cactttgttg
    31201 ccacctttat cggggagtca aacatcttgc caggtaccat gattgaggac tacttggtcg
    31261 aatttaacgg caaacgcttt gaagcggttg atggtgggat gaagccaaat gaacctgttg
    31321 aggtcgttat tcgtccagag gacttgcgca ttacccttcc tgaagaaggc aagctccaag
    31381 ttaaggtcga tacccagctt ttccgtggag ttcattatga aattatcgcc tatgacgaac
    31441 ttggaaatga atggatgatc cactcaaccc gtaaggctat cgtgggtgag gaaatcggtc
    31501 tggactttga accagaagac atccacatca tgcgtctcaa tgaaaccgaa gaagagttcg
    31561 atgctcgtat tgaggagtac gtagaaatcg aagagcaaga agcaggtttg atcaatgcaa
    31621 tcgaggagga aagagatgaa gaaaacaagc tctaaactct ttgtagtacc ctacatgctt
    31681 tggattgcgc tctttgtatt ggcacccttg gtcttgattt tcggtcaatc ctttttcaac
    31741 atcgaaggcc agttcagttt agaaaattac aaatcttact ttgcgtcaca aaacttgacc
    31801 tatcttaaaa tgagtttcaa ctcagtgctt tatgcaggca ttgtgacctt tgtggcactg
    31861 cttatcagtt atccgacggc cctctttttg acccgtctca aacaccgtca actctggctc
    31921 atgctgatta tccttcctac ctggatcaat ttgctcctta aagcctatgc ttttatcggg
    31981 atttttggtc aaaatggctc tattaaccaa ttcttggaat ttatcggaat tggttcacaa
    32041 cagttgcttt ttaccgattt ttcctttatc tttgtcgcaa gctacatcga gctccccttt
    32101 atgattttgc cgattttcaa tgtcttagac gatatggata ataatctcat caatgctagt
    32161 tatgaccttg gtgcaactaa gtgggagacc ttccgtcatg tcatcttccc tctatctatg
    32221 aacggtgtgc gaagtggggt tcagtcggtc tttatcccaa gtttgagtct cttcatgctg
    32281 acccgtttga ttggtgggaa ccgcgttatc accttgggga cggctattga gcagaatttt
    32341 ctaaccaatg acaactatgg tatgggttca accatcggtg tgattctcat cctgaccatg
    32401 ttcatcacca tgtgggtgac taaggaaagg agagaacgat gaaaaaattt gccaaccttt
    32461 atctgggact ggtctttctg gtcctctacc tgcctatctt ttacttgatt ggctatgcct
    32521 ttaatgctgg tgatgatatg aatagcttta caggttttag ctggactcac tttgaaacca
    32581 tgtttggaga tgggagactc atgctgattt tggctcagac atttttcttg gccttcctat
    32641 cagccttgat agcgaccatt atcgggactt ttggtgccat ttacatctac cagtctcgta
    32701 agaaatacca agaagccttt ctatcactca ataatatcct catggttgcg cctgacgtta
    32761 tgattggtgc tagcttcttg attctcttta cccaactcaa gttttcactt ggctttttga
    32821 ccgttctatc tagtcacgtg gccttctcca ttcctatcgt ggtcttgatg gtcttgcctc
    32881 gactcaagga aatgaatggc gacatgattc atgcggccta tgacttggga gctagtcaat
    32941 ttcagatgtt caaggaaatc atgcttcctt acctgactcc gtctatcatt actggttatt
    33001 tcatggcctt cacctattcg ttagatgact ttgccgtgac cttctttgta acaggaaatg
    33061 gcttttcaac cctatcagtc gagatttact ctcgtgctcg caaggggatt tccttagaaa
    33121 tcaatgccct gtctgctcta gtctttctct ttagtattat cctagttgta ggttattact
    33181 ttatctctcg tgagaaggag gagcaagcat gaaaaaaatc tattcatttt tagcaggaat
    33241 tgcagcgatt atccttgtct tgtggggaat tgcgactcat ttagatagta aaatcaatag
    33301 tcgagatagt caaaaattgg ttatctataa ctggggagac tatatcgatc ctgaactctt
    33361 gactcagttt acagaagaaa caggaattca agttcagtac gagacttttg actccaacga
    33421 agccatgtac actaagataa agcagggtgg aacgacctac gatattgcca ttccaagtga
    33481 atacatgatt aacaagatga aggacgaaga cctcttggtt ccgcttgatt attcaaaaat
    33541 tgaaggaatc gaaaatatcg gaccagagtt tctcaaccag tcctttgacc caggtaataa
    33601 attctccatc ccttacttct ggggaacctt aggaattgtc tacaacgaaa ccatggtaga
    33661 tgaagcgcct gagcattggg atgacctttg gaagccggag tataagaatt ctatcatgct
    33721 ctttgatggg gcgcgtgagg tgctgggact aggactcaat tccctcggct acagcctcaa
    33781 ctccaaggat ctgcagcagt tggaagagac agtggataag ctctacaaac tgactccaaa
    33841 tatcaaggct atcgttgcgg acgagatgaa gggctatatg attcagaata atgttgcaat
    33901 cggcgtgacc ttctctggtg aagccagcca aatgttagaa aaaaatgaaa atctacgtta
    33961 tgtggtaccg acagaggcca gcaatctttg gtttgacaat atggtcattc ccaaaacagt
    34021 taaaaaccaa aactcagcct atgcctttat caactttatg ttgaaacctg aaaatgctct
    34081 ccaaaatgcg gagtatgtcg gctattcaac accaaaccta ccagcgaagg aattgctccc
    34141 agaggaaaca aaggaagata aggccttcta tcccgatgtt gaaaccatga aacacctaga
    34201 agtttatgag aaatttgacc ataaatggac agggaaatat agcgacctct tcctacagtt
    34261 taaaatgtat cggaagtagg agataaacgt aagaagacga atcagtcaag ctggttcgtt
    34321 tttaaggaat agaagtcagt ttttagagcc ctatcttttt ctattctagt tgtttatttg
    34381 tttatttgct tgcctagtag tatactaagc ctatactctt caaaccacgt cagcgttgcc
    34441 ttgccgtact caagtacagc ttgaggctag cttcctagtt tgctttttga tttttattga
    34501 gtattaacct tacagaaagc ggcaacaaat aatgaaaatg tctacttttt tcaaaaaaag
    34561 tttctggcca acctttatga ttgttaacca aactgctata ctattccatt taaaggatgg
    34621 cttggatcga caatacctaa caactgagtc catatattgg gtgataggca cctttatatt
    34681 tggaaatatt ctagtagctg tgtttagtaa tatgaaaatt tgggataaaa agaaaaatgg
    34741 cagtaagaaa aaatatatcc tgaaaaagta atcagagatg tagggaattg gtctaattca
    34801 acggagatca tttccagttg acgcaagctt aaaaaaacgt tataataaga aagttgagaa
    34861 ttgattttca cttgtcttag tccagagaaa tggcggtgct gcgagccatc taagctagga
    34921 agtcatgcta ctcaatcata caattgcata agaacaagag aatgacaagt tcattgaatg
    34981 aaggtggtac cgcggttttt cgcccttcgt gatatgagct tgtcttttga tttttggagg
    35041 tgttgatgaa aacatttctt gtcaaacaaa agtttcgtct tggaggcgaa cgcttcgcta
    35101 tcaaggatga caggggagaa attgcctatc aggtggaggg atcatttttt aagattccta
    35161 aaacctttac catctatgat gcggctggtg aacaagtcag tgagattagt aaagaaatct
    35221 tgaccttgct tcctcgtttt gaaattcagc ttcgggatgg ctcgagtttt gtcattcgta
    35281 agaagttgac cttctggcga gataagtatg agtttgataa tctaggtctt cgtatcgagg
    35341 gcaatatctg ggatttgaat ttcaaattgc tggatgatcg cgatcagttg attgcggaaa
    35401 ttaagaagga actcttccat ctgacttcta cctataccgt aacggttctt gaagacgctt
    35461 atgcagacct agtcatttcc ctctgtgtcg cgattgacta tgtggaaatg ctggaaagcc
    35521 aatcacatta aacaagtgaa taaggagaca atatgaaaca actatctagt gcacaagtac
    35581 gccaaatgtg gcttgatttc tgggcgacca aaggtcactc agtagaacca tcagtaagtt
    35641 tggttcctgt aaatgaccca actcttttgt ggatcaactc tggggtagca acccttaaga
    35701 aatactttga cgggaccatt atccctgaaa atccacgtat taccaatgcc caaaaggcta
    35761 tccgtaccaa cgacatcgaa aacgtaggga agaccgcgcg tcaccatacc atgtttgaaa
    35821 tgctggggaa cttctctatc ggggattact tccgtgacga agctatcact tgggcttatg
    35881 agcttttgac aagccctgaa tggtttgatt tccctgctga aaaactttac atgacctact
    35941 atccagacga taaagattct tacaaccgct ggattgaagt gggagtggat ccaagtcact
    36001 tgattccaat tgaggacaac ttctgggaaa tcggtgcggg accttctgga ccagatacag
    36061 aaatcttctt tgaccgtggg gaagcctttg acccagaaaa tatcggtctt cgcctgcttg
    36121 cagaagatat tgaaaacgac cgttatattg aaatctggaa catcgttttg tcacaattta
    36181 acgcagaccc tgctgttcct cgtagcgaat acaaggaatt gccacataag aacattgata
    36241 cgggcgctgg tttggagcgt ttggtggccg ttatccaagg ggctaagacc aactttgaaa
    36301 cggacctctt catgccgatt atccgtgaag tcgagaaatt gtctggtaag gtttatgacc
    36361 aagatggcga caacatgagc ttcaaggtta tcgcagacca cattcgttct ctttcatttg
    36421 ctatcggtga tggtgccctt ccaggaaatg aaggtcgtgg ttatgttctt cgtcgtcttc
    36481 tccgtcgtgc ttctatgcat ggtcaaaaat tgggtatcaa cgagcctttc ctttacaaat
    36541 tggttccaac tgttggaaaa atcatggaaa gctactaccc agaagtgctt gagaaacgtg
    36601 actttattga gaaaatcgtt aagagcgaag aagaatcatt tgcccgtacc cttcactcag
    36661 gtcaacactt tgcccaaggc attgtagcag acttgaaaga aaaaggtcaa tctgttatcg
    36721 ctggttcaga tgtcttcaaa ctctatgata cttatgggtt cccagttgaa ttgactgaag
    36781 aaatcgctga agaagctggt atgactgtag accgtgaagg ttttgaagca gccatgaaag
    36841 aacagcaaga acgcgcgcgt gcgtcagctg tcaagggtgg ctcaatgggt atgcaaaatg
    36901 aaactcttca aaacatcact gtagaaagtg tcttcaacta caatgctagc caattgtctt
    36961 ctaaattggt agctatcgtt gctgacaatg cagaagtagg agctgtttca gaaggaactg
    37021 cctctcttat ctttgcggaa acgtcatttt atgctgaaat gggtggacag gtagctgact
    37081 acggacaaat cttggatgag tcaggtaagg ttgtggctac tgtgaccaat gttcagaaag
    37141 ccccaaatgg tcaagccctt catacagttg aagtccttgc accgcttgcc ttgaaccaag
    37201 aatatacctt ggcaattgat agcaatcgcc gtcaccgtgt catgaaaaac cacactgcga
    37261 ctcatttgct tcacgctgcc cttcacaata tccttggaaa ccacgcaaca caggcaggat
    37321 ctcttaacga agttgaattc cttcgctttg actttaccca cttccaagca gtaactgctg
    37381 aggaacttcg tgcgattgaa cagcaagtta acgagaaaat ttgggaagca cttgaagtga
    37441 agacagttga aacggatatt gacactgcta aagaaatggg agctatggcc ctctttggtg
    37501 agaaatacgg caaggaagtt cgtgtcgtga ctatcggtga ctactctatt gaactttgtg
    37561 gtggtactca tgttgacaac acttctgaga ttggtctctt caaaattgtc aaagaagaag
    37621 gaatcggttc aggaactcgc cgtatcttgg cagtgactgg taaggaagcc tttgaagcct
    37681 atcgtgaaca agaggatgct cttaaagcta tcgcagcaac cttgaaagca cctcaagtca
    37741 aggaagtacc tcacaaggta gaaggacttc aagaacaact tcgtcaactt caaaaagaaa
    37801 atgctgagtt gaaagaaaaa gccgcagctg cagccgcagg cgatatcttc aaagatgtta
    37861 aggaagtcaa cggtcatcgt tacattgcta gtcaagtgtc tgtatccgat gccggtgccc
    37921 ttcgtacttt tgcagataac tggaaacaaa aagactactc tgatcttctt gtcctagttg
    37981 ccgctatcgg tgacaaagtc aatgtccttg tagcaagcaa gacaaaagac cttcatgcag
    38041 gaaaccttgt caaagaatta gcaccaatca tcgatggacg tggtggtggt aaaccagata
    38101 tggccatggc aggaggaagc aaccaagcta agatccaaga attgttggat gcagtagcag
    38161 gtaaattgta atcaagtaaa gatctatcca tttgggtagg tctttttgtg aatacaaaaa
    38221 agccaaatcc gcttggacct agcttggtta ttatgagcta ttagatggta ttgactgccc
    38281 agacacttac ggatctagct gagacaggga attgtccata accttcctca tcaattgtaa
    38341 cttgaccttg gtggttacca agtaaatcta caaaggtttg attagtccat tcttgaccga
    38401 caaacattga cttgctgttt tcttggtcat ttgagataag gactgcgatt ggggattgat
    38461 tttcagcacc tgaacgtacc caaccgatac agttagcatg gtcaaagtag tcattttgtt
    38521 ctccataggc caaatctttt cggatggcta ggaggcggtc aaggatttct ttgaaatctt
    38581 cttgagcata ctgccctgaa atcccatagt agtctccgta aaagacacat ggaaggccgt
    38641 cttggcgtaa caaaatgagg gcataggctg ctggcttgaa ccattcttca acggtagact
    38701 caagggcttg tcctcgttgg gtatcgtggt tgtcgacaaa agtcacagcc ttgtcaggct
    38761 tgagttcaac caggctatct gtgaaaatgc cacgaaggtc atagtttgcg ccagcttgac
    38821 tggcttcaaa gagattctgg tggagacgaa catcgacaag gtcaaagtgt tcttccgttt
    38881 tttcgagata gtccagattg gcttccttgt ctgggttcca aaattcacca aaaacataga
    38941 aatcgtcacc gtatttttcc ttcatatcgc ggatgaagtt gcgcataaag aaagagtcaa
    39001 tatgcttaac ggcatccaaa cggaaaccag ctacaccagt cgtttccatg aaccaatcag
    39061 cccagtcata gatgttttgg atgacttcag gatgtttaaa gtctaggtcg gcatacatga
    39121 ggtagtcgta gtttccgttt tcgttatcga ccaattcctc gttggcccag cccttgttgt
    39181 ccccttggat cagataaatc ccagatttac tgcgtttggc atcgtagtct gtaccggtga
    39241 agtggtacca atgccagtgg aagccattat aggtatcttg gcgaccatcg aaggtaaaac
    39301 tagtccagcc attgatggtg aagggttctc caagttcaac tgtacggtct acaggatcaa
    39361 cttcgataac ctgaaaggct tccctgtgat cggcagcagc cttgtggttg agaactacat
    39421 cggccatagg ttgaattccc tgtgctttaa gggcttgaat ggcttgaaga tagtcttctt
    39481 tgaaaccata cttggtgcgg acagtccctt tttggttgaa ctctcctaag tcaaataagt
    39541 catagacccc atagccgaca tctttttcgt tggttgcctt gaaggctggt ggcatccaga
    39601 cgtgactgat ccccagatga gctaggtgtg gagcattttc agccagacgc gtccagtgtt
    39661 gaccgtcgtg gggcagatac cattcaaagt attgcatgag tgtttgattt tgcataatgt
    39721 ttcctcttac ttgtcaatct tgttctttat tctatcataa agtatctatt agcgcaaacg
    39781 tttgcgtaag atagaaggaa actattttaa ctacttaaaa taaagtattg atttttttgt
    39841 atcaaagtgc tagtatagcg atatataata cagataaaga taggagttgt cccatgattg
    39901 aatttcaaaa tgttagtaag ttgtatggtg ataaagaggc cttgagtaat ctcaatttgc
    39961 agattgaaaa tggagatatt atgggcttga ttggtcataa tggggctgga aaatcgacca
    40021 ctataaaatc cctagtcagt atcatttcac ccagcagtgg tcgtattttg gtagacggtc
    40081 aggagttatc ggaaaatcgc ttggctatta aacgaaagat tggctacgta gcagactcgc
    40141 ctgacttatt tttacgctta acggccaatg aattttggga attgatcgcc tcatcctatg
    40201 atctgagtag atctgacttg gaggctagtc tagctaggct attgaacgtt tttgattttg
    40261 ctgaaaatcg ctatcaggtt attgaaactc tttctcacgg aatgcgtcag aaagtctttg
    40321 tcatcggagc actcttgtct gatcccgata tttgggtctt ggatgaaccc ttgactggtt
    40381 tggatcccca ggctgccttt gatttgaaac agatgatgaa ggaacatgca caaaaaggga
    40441 agacagtctt gttttcaact catgtcctag aggtggcaga gcaagtctgt gatcggattg
    40501 ccattttgaa aaaggggcat ttgatttatt gtggtagtgt agaggacttg agaaaagatt
    40561 acccagacca gtctttggaa agtatctacc ttagtcttgc tggtagaaaa gaggaggttg
    40621 cggatgcgtc tcaaggtcat taaaaaatta gttgatatca atatccttta ttcatctcaa
    40681 gaagctaatc tggctaatct acgaaagaag caggctaaga atcctgggaa aaaagtaaat
    40741 gtttccgcta gagtcttaag ttcttacatt ttttccagtc tcttgatgat catctgtttt
    40801 agtaatatag ccattcattt tccttttgag gaaataccta tttattttag ctcgatgatt
    40861 gctattttac tagtgattgc cttttcaact tctttaacgg ccttttacaa tgtcttttat
    40921 gagagtaagg atttggtatc gtataggccc tatgccttta aagaatcaga gattataatt
    40981 gctaaaggac tgtctgtcct tttgccagct ctaactggaa ttgtaccaat cctagcttat
    41041 tttctggtcc tctacattag gctagctcct tctctatggc tgggtttgcc tttgatgcta
    41101 ctgtccttga ccttattatt tgtctctgtg gctctagtga tggtagtggc agtgcatttc
    41161 ttggctcaaa ctagggtctt cagaaagtat cagtctattt tttcgaatgt gatgattggg
    41221 ataggagttc tcataccttt aatatttatc ttctttcttc agtcgacttt tggaagtatt
    41281 gttgacaaag ttagagacat tccatttctc ctttatcctc ttcatatctt ttacaaaata
    41341 gcagtggagc ctttttcgac agaagcctta gtgggtctgc tcgcttggat aggactaact
    41401 ctcttcctgc tttatctgac caaaaagaag gtccttcctc gtttttatga cgtgatcctg
    41461 cttaatagtg aggagaaggt caaaaaagaa cgtcgcagca aggagagaat ttcaaccact
    41521 aaaaagggat tttttcgcat ggttttacgc taccacctca ccctcttggg acaggggact
    41581 ggcgtgatta cagtgctttt tacaagtgct ttccttcctt atctcatgat gatcggtctg
    41641 atttccaaaa tccgagattc tcagatagtt ccagacattc atcctccata ctggttaccc
    41701 ttgtttttta tagctctctt tatagcagtt gtcaataaca atatcaccag cctgcattca
    41761 attgccttgt ccttggagag ggaaaatgtt gattttctta agagtttacc ctttgacttt
    41821 gctcgttatg tgaaagtgaa attttggatt atatatgctg tccagtcctt tttaccagtt
    41881 ctgactttgc ttggtctttc tctatatcta ggcttgccca tcatttcgat gatttacctt
    41941 cttgtagtct ggatcattgc cagtgtcatc ctttcttgcc accattactt taaggacgtt
    42001 aaaaatctgt caaccaattg gagtagcatt acggacctgg tgaatcgttc aaatggcata
    42061 gtcgccatag ttttattgtt tatttatagt gcaatcctga tggcccttgt aattgggagc
    42121 atattcttgg ttcagtctct ctcccctatc cttgccatca gcttgggagt aggagctctt
    42181 atcgtcttgc ttgctcttgc tatttttggc tatcattatt acctgtcacg catattggca
    42241 gaaatagaaa aaagatgaac tggttggtcg cttgcttgat aaattcaatt cgacaaacgc
    42301 ttttatagtt tgttagaatg tagttgtaaa tgattaagat tggtaatcaa aaatatatag
    42361 ggaggaatgt tttgaataag aaatataaca tagttctatt tttgttgttt atagtttatt
    42421 tatttggata tttttcaatt tcaaaaacgt taattcctat aatgtgtgtg ttccaagtat
    42481 ttttgataga acatatattt agagttcgaa atagaatgat gcagataggt gaaattataa
    42541 ttattgttgc ttctataata ttatttattg atagtatatt gagtttgtaa caaaaggctt
    42601 ctctataaaa tcaaattttc cagataccta ctgaagctga tttatttctt tatctttctt
    42661 ctactacctt tgtcactaaa tttgacagac acatattgct gatacaacct ctctacctat
    42721 aatctcacag ttaacctgag gctagtttca tagtttgcta tttgattttc gttgataaaa
    42781 agaaatgctc ctccaagacg gaagagcatt ttttactgtg attcttacac atttacttcc
    42841 tagttttgca ggaagtctag tataccattt tttaaaagaa gagtaaaaaa gcaagggtag
    42901 tttctttttt gagaattgtc taaaagtttg atataatggt agttatgaat agaataagag
    42961 tcagcaaaag ggttgaaaag aagcttgcta aggggctagt tttactagaa gccagtgatc
    43021 ttgagaatgt caatcttaag gatcaggaag tagaggtgca gggtcaggaa ggaaactttc
    43081 ttgggactgc ctacctttct cagcaaaaca agggcttggg ctggtttatc agcaaagaca
    43141 aggtggcctt caatcaagct ttctttgaaa cgttgtttag aaaagccaaa gaaaagagaa
    43201 acgcctacta tcaagatgat ttgacaactg cctttcgtct ctttaatcaa gagggagatg
    43261 gctttggggg tctgacagtg gacctttatg gcgactacgc cgtcttttct tggtataact
    43321 cttatgttta tcagattcgt cagactatat cagaagcctt tagacaggtt ttccctgagg
    43381 ttttaggagc ttatgagaaa atccgcttta agggtttgga ctatgaatct gcccatgttt
    43441 atggtcaaga agcacctgac tttttcaatg ttttagaaaa tggtgtcctg tatcaagtct
    43501 ttatgaatga tggcttgatg acaggaattt tcctagacca gcatgaggtt cgcggtagtt
    43561 tagttgacgg cttggctatg ggtaaatcct tactcaatat gttttcctac acagcggctt
    43621 tttcagtagc tgcggccatg ggaggagcta gccatacaac ttctgttgat ctagccaaac
    43681 gttcacgaga attgtctcaa gcgcattttc aggcaaatgg gctcagcaca gacgagcatc
    43741 gttttatagt catggatgtc tttgagtatt tcaaatatgc caaacgcaaa gacttgacct
    43801 acgatgtgat tgtcctagat ccgcctagct ttgctcggaa taaaaaacaa actttctctg
    43861 tggccaagga ttatcacaag ttgatttccc agagtcttga gattttaaat ccgggaggga
    43921 ttatcattgc cagtaccaat gctgccaatg tttcccgtca gaaatttaca gaacaaattg
    43981 ataaaggttt tgcaggaaga agttaccaga ttttaaacaa atatggtctt ccagcagatt
    44041 ttgcctataa taaaaaagat gaaagtagta attacctcaa ggtgattagt atgaaggtta
    44101 gtaaatgaaa ttaatcgttt cagtaatgcc aagaagttta gaggaggctc aggctctgga
    44161 tgccacgagg tacctggatg ccgacatcat tgaatggcgt gccgactatc tgcctaaaga
    44221 agcgattttg caggtagctc cagccatttt tgaaaaattc gcaggccgtg agttggtttt
    44281 cacgctacga actcgctccg aaggtggaga aatcgacctt tctccagaag agtatatcca
    44341 tctaatcaag gaagttgcgc aactctatca accagactat attgattttg agtactatag
    44401 ctacaaggat gtttttgagg aaatgctgga cttcccaaat cttgttttga gttaccataa
    44461 tttccaagaa acacctgaga acatgatgga aatcttgtca gagttgacga tcctaaatcc
    44521 aaaacttgtt aaggttgcgg tgatggctca cacggagcag gatgtcttag acttgatgaa
    44581 ctatacacga ggctttaaaa ccctcaatcc tgaacaggaa tatgtgacca tttctatggg
    44641 taaggtgggc aaggtctctc gtatcactgc ggatgtgact ggttcgagtt ggtcttttgc
    44701 cagtctggat gaggttagtg cccctgggca gatttctcta gctagcatga aaaaaatcag
    44761 ggagattttg gatgaagctt gatggctata cacgtttagc tgcagttgtt gccaatccta
    44821 ttaagcattc tatttctccc ttcatccaca atagagcctt tgaggcgaca gctaccaacg
    44881 gtgcttatgt ggcttgggag attgaagcga gtgacttggt agaaacagtg gccaatattc
    44941 gtcgctacca gatgtttggc atcaatctgt ccatgcccta taaggagcag gtgattcctt
    45001 atttggataa gctgagcgat gaagcgcgct tgattggtgc ggttaatacg gttgtcaatg
    45061 agaatggcaa tttaattgga tataatacag atggcaaggg attttttaag tgcttgcctt
    45121 cttttacaat ttcaggtaaa aagatgaccc tgctgggtgc aggtggtgcg gctaaatcaa
    45181 tcttggcaca ggctattttg gatggcgtca gtcagatttc ggtctttgtt cgttccgttt
    45241 ctatggaaaa aacaagacct tacctagaca agttacagga gcagacaggt tttaaagtgg
    45301 atttgtgtgc tttagaatat gtttctgaac tgcaagcaag gattgccgag tcggatttgc
    45361 tagttaatgc caccagtgtg ggcatggatg gccaatcctc tcctgttcct gaaaacatag
    45421 tcttaccaga aactctttta gttgcagata tcatatacca accctttgaa acaccgtttt
    45481 tgaaatgggc tagaagtcag ggcaatccag cagtcaatgg tctgggaatg ttactctatc
    45541 aagctgcaga agcttttcaa ctgtggacag gcaaggaaat gccgacagaa gagatttggc
    45601 agtctttaac agaaaaatac caataaagaa agggagactc tcctatgaaa atcagaatcg
    45661 atattcctca ccatccttat gatattcaga ttgaaaaagg ttgtatggcc caggctggtc
    45721 agtggttgcg agaactctgg caaccgcaaa aggtagtcat tgtgacagat aaccatgtag
    45781 cctctctcta tgcagagaag gtcaagctca gcctagaaga tgctggtttt caggtagctg
    45841 tttttgattt cttagaaggt gaagaaagaa agaatttaac tactgttcag aaagtctatg
    45901 aatttctagt caagcaaggt ctgactcgta gcgatggaat cgttgctctt ggtggtggcg
    45961 ttgttgggga cctggctgga tttgtagcct ctacctatat gcggggtatt cactttgttc
    46021 agattccgac tagtttgaca gctcaggttg attcttctat cggtggaaag acaggcgtta
    46081 atacaccatt tgctaaaaat atggtgggga cctttgccca accagatggg gttctgattg
    46141 atccacttgt tcttgaaacc ctcggaaaaa gagagttgat tgaagggatg ggtgaagtta
    46201 tcaagtatgg cttgattgag gatccagaac tgtgggctct cttgacggga ctgaatggtt
    46261 ctgttgagag tattttggaa catgcagaga ccttgattga acattcttgt caggtgaagc
    46321 gcaagatggt ggttgaagat gagttggaca atggtattcg tctttacctc aattttggcc
    46381 acactattgg ccatgccatc gaagcgactg ccggttatgg caaggtcatg catggagagg
    46441 ctgttgccat gggaatggta cagatttcca aggttgctga ggaaaaaggc ctcatgccag
    46501 ctggcattac ccaatctatc acagagatgt gtcagaaatt cggtttgcct gttgactatg
    46561 aaaattggga agttgacaag ctttatcagg ctcttactca tgacaagaaa gcgcgtggta
    46621 acaccttgaa attggtcttg gtgccagagc ttggttcagc gaccattcac ccagtttctc
    46681 tggaagagat gaaagactac ttggtaaaat aaggagagtg tatgagatat ttaactgcag
    46741 gagaatcaca cggcccccgt ctaacagcta ttattgaggg aattccagct ggacttccat
    46801 tgacagctga ggatatcaat gaggacctta gacgccgtca gggtggctac ggtcgtggtg
    46861 gtcgtatgaa gattgagaat gaccaggttg tctttacttc gggcgttcgc cacgggaaga
    46921 cgacaggggc gcctattact atggatgtca tcaataagga ccaccagaaa tggctggaca
    46981 tcatgtctgc ggaggacatt gaagaccgcc ttaaaagcaa gcggaaaatt actcatcctc
    47041 gcccaggtca tgccgatttg gttgggggga ttaagtaccg ttttgatgat ttgcgaaatt
    47101 ctttggagcg ttcatcagct cgtgaaacca ccatgcgggt ggcagttggt gcagtagcca
    47161 aacgcctctt ggctgagctg gatatggaga ttgccaacca tgtcgtggtc tttggtggca
    47221 aggaaatcga tgttcctgaa aatctgacag tcgctgaaat taagcaaaga gctgcccagt
    47281 ctgaagtttc tattgtcaac caagaacgag aacaggaaat caaggactat attgaccaaa
    47341 tcaaacgtga tggtgatacc atcggtgggg ttgtggagac agtcgtcgga ggcgttccag
    47401 ttggtcttgg ttcctatgtc caatgggata gaaaattgga tgcaagattg gctcaagctg
    47461 ttgtctctat caatgccttt aaaggggtgg aatttggtct tggctttgag gctggttatc
    47521 gtaaaggcag ccaagttatg gatgaaattc tctggtctaa agaagacggt tatactcgcc
    47581 gtaccaataa tctaggtggt tttgaaggtg gtatgactaa tgggcaaccc atcgttgttc
    47641 gtggggtcat gaaacccatt cctactcttt ataaacctct tatgagtgtg gatatcgaaa
    47701 cccacgaacc ttacaaggca accgtggaga gaagtgatcc gactgctctt ccagctgcag
    47761 gaatggtcat ggaagcagtt gtagcaacgg ttctggcgca agaaatcctc gaaaaattct
    47821 catcagataa tcttgaggaa ctaaaagaag cggtagccaa acaccgagac tatacaaaga
    47881 actattaagg agttcctatg gcaaaaacaa tctatatcgc aggtcttggg ttgattggag
    47941 cctctatggc acttggtatc aaacgcgatc atccagatta tgaaatttta ggttataatc
    48001 gtagtcaagc ttcgagagat atcgccttga aagaaggtat gattgaccgt gcaacggatg
    48061 attttgctag ttttgctcct ttggcagatg tcattatcct cagcttgcca atcaaacaaa
    48121 ctattgcttt cattaaggag ttggccaatt tggatttgcg agaaggcgtt attatttcag
    48181 atgctggttc gaccaagtca accattgtgg atgcggcgga gcagtatttg gctggcaagt
    48241 ctgttcgctt tgtcggggcc catcccatgg ctggtagtca caagacaggg gctgcttcgg
    48301 cagatgtcaa tctttttgaa aatgcctatt atatctttac accttcaagc ctgacaagtc
    48361 aggacacgct taaggaaatg aaggatctgc tttcaggtct tcatgctcgt tttatcgaga
    48421 ttgatgccaa ggagcatgat cgtgtcactt ctcagattag ccattttcct catattttgg
    48481 cttctagtct catggagcag actgcggtct atgctcaaga gcatgagatg gcaaggcgct
    48541 ttgcggcagg tggttttcga gatatgaccc gaattgcgga aagcgagcca ggaatgtgga
    48601 cctccattct cttgtccaat agcgagatca ttctggatag aattgaggat ttcaaggaac
    48661 gtttggaagc gattggtcag gccattagta agggagatga agagcaaatt tggaactttt
    48721 ttaaccaagc gcgtgaacaa cgccagacca tggaaatcca taagcgtggt ggtgtggata
    48781 gctcttacga cctctatgtt gacgttcctg atgaagaaga tgtcatcctg cggattttgg
    48841 aactgctacg tggaacttcc ttggttaata tccacatcaa cgaggaaaat cgtgaggata
    48901 ttcacggaat cctacaaatt tcatttaaaa atgctcaaga cttggaaaga gctgagcatc
    48961 tcataacaga aaatactgac tatacagtcg tcatcaaata aggagaagaa catgtcaaat
    49021 atttacgata gtgcaaacga actcagtcgc ggtctacgcg gattaccaga atacaaggct
    49081 gttaaagcag ctaaagatgc gattgcagca gatgctgagg caagcaaaat ctttacagat
    49141 tatcttgcct ttcaagagga aattcaaaaa ctagcacaga caggtcaaat gccagatgcg
    49201 tccttccaag ctaagatgga aggctttggt aaacagattc aaggaaatag cctcttgtca
    49261 gaattcttta ccaagcaaca acaattggcc atttaccttt ctgatattga aaaaattgtt
    49321 tttgaaccag tttcagaatt gctgaaataa gaaaagaact atcctaaagt caggaatttt
    49381 taaggaattt ctggcttttt atgataaaat aggtaaaagt attgcaagta tgaggtccag
    49441 tatgaaacta aaaacaaaca ttcgccattt acatggtagt atccgcgtcc caggtgacaa
    49501 gtctatcagc caccgttcca ttatctttgg aagtttggct gagggtgaga ccaaggttta
    49561 tgatattctg cgaggtgaag acgttctttc gaccatgcag gtttttcgtg accttggtgt
    49621 tgaaattgag gataaagatg gggttattac cattcaaggt gtaggcatgg ctggcttaaa
    49681 agcgccacaa aatgccctta atatgggaaa ttctggcacc tcgattcgcc tgatttcagg
    49741 tgtccttgct ggtgcagatt tcgaagtaga gatgtttgga gatgatagtc tttccaaacg
    49801 tcctatggac cgtgtgaccc ttccactgaa aaaaatgggc gtcagcattt cagggcaaac
    49861 tgaacgagac ttgcctcccc ttcgcttaaa agggacgaaa aacctaagac ctattcatta
    49921 tgagttgcca attgcctctg cccaagtcaa gtcagccttg atgtttgcag ccttacaagc
    49981 taagggggag tcagttatta tcgaaaaaga gtacacccgt aatcatactg aagatatgtt
    50041 gcaacaattt ggtggtcatt taagtgtgga tggtaagaaa atcacagtcc aggggccaca
    50101 aaaattgaca ggacagaagg tggtcgtacc aggagatatt tccagtgcag ccttttggtt
    50161 agtcgcaggt ttgattgctc caaattctcg tctagtgctg cagaatgtgg ggatcaacga
    50221 aactcgcacc ggtattattg atgtcattcg tgccatgggt ggaaaattgg aaataactga
    50281 aatcgatcca gtcgctaaat ctgctacctt gattgttgag tcttctgact tgaaaggaac
    50341 agagattggt ggcgctttga ttccacgatt gattgatgaa ttgcccatta ttgccctgct
    50401 tgcgacccaa gcccaaggtg taacagttat caaggatgct gaggagctca aggtcaagga
    50461 aacagaccgt attcaggttg tggcagacgc cttaaatagt atgggagcag atattactcc
    50521 tacggcagat gggatgatta tcaaaggaaa atcagctctt cacggtgcta gagtcaatac
    50581 gtttggtgac caccgtatcg gcatgatgac agctatcgca gccctcttgg ttgcagatgg
    50641 agaggtggag cttgaccgtg cagaagctat caataccagc tatcctagtt tctttgatga
    50701 tttggagagc ttgattcatg gctaaggtgt tattagggtt tatgggggct ggaaaatcga
    50761 ctattgcaag aggattggac cctaattacc ttgatatgga tgctctgatt gagaagcgcc
    50821 taggtatgtc cattgcgaat tttttcgctg aaaagggaga agagaccttt cgtcaggtag
    50881 aatcagaagt cctagctgat ttactacaaa cagatcaagt cgtgtcaact ggaggaggag
    50941 tggttatttc tcagagaaat cgtgacttac tcaagactaa tacagataac atctacctga
    51001 aagcagattt tgaaaccctc tacctacgta tcgcagctga taaggacaat cagcgaccgc
    51061 tttttctaaa taatagcaag gaagaactag tagctatttt tcaagaaaga caggcttggt
    51121 atgaggaagt ggctagtcgg gttttggatg tgaccaagct aagcccagag gaaattatag
    51181 aggaactaag atgaaaattg cttatctagg tcccaaggga tcattttcac accacgttgt
    51241 gcagacagct tttcctcatg aggaattgca ggcttttgcc aacattacag atgtcatcaa
    51301 ggcttatgag caaggattag tggactattc tgtggtgcca gttgaaaatt ctattgaggg
    51361 tagtgttcat gaaaccttgg actatctttt tcatcaggct cacatccaag cagtagcaga
    51421 aatcgttcag cctattcatc agcagttgat ggtggttcca ggccatacta agattgaaaa
    51481 gattttttca catccacagg ccttggctca aggaaagaaa ttcatcgatg aacaatatac
    51541 agaggctcaa atcgaggtaa cagctagtac agcttatgca gcccgtttta tttccgaaca
    51601 tccagatcag ccctttgcag caattgcacc tagaagttct gctgaagaat atggattgga
    51661 actgattgcc gaggatattc aggaaatgga agccaatttc acacgtttct gggttctagg
    51721 agctgaaaag cctagtattc ccttgcaagc acaaactgaa aagatgagtt tggccttgac
    51781 attacctgac aaccttccag gtgcacttta taaggccctg tcgacctttg cttggcgagg
    51841 aattgacttg acaaaaattg aaagtcgtcc actcaagaca gcactgggtg aatacttttt
    51901 cattatcgat gtggattata ccgataagga cttggtccac tttgcccaaa aagaattaga
    51961 agcgattgga atccagtata aaattctggg tgcctatcct atttatccaa tatcagacca
    52021 tggaaaggag agaagatgag taaagaaaat ctcttaagtc atcatgagca gttgcgttat
    52081 gattatttgc taaaaaatat tcactatctc aatgagagag aaaaaaatga gtttgcctat
    52141 ttgcaagaaa agctaactct tgctagggga aatagtagct ctagcttgga acaagaaaga
    52201 gaagagcagg ttgacttacc aagctatgcg aaccggagtc gctcacaatc caaatcacaa
    52261 gcactctctt tccctccaaa aaagaaaaga cggaagctcc gtcttaaacg aatttttatg
    52321 gtgatttttt cacttttggt ctgtgtggct ttggccatgg tattcatgtt tttgcgtggt
    52381 taccaagatg ctagcgcaaa gaaaactgct gatgcccggg cagctcaagt agaagtcttt
    52441 aatggtcagg acactagaga tggagttaat attttaatca tgggtactga tggtcgaatc
    52501 ggccagaaca gtgttgagac acgaactgac tctattatgg tattaaatgt cgggggctca
    52561 gataagaaaa tgaagctagt cagtttcatg cgtgataatt tggtctatat agatggttat
    52621 agtcaagtga ttaacggtag aaaacagaca gataacaagt taaacgtagc ctacgagtta
    52681 ggagaacaag aggggcaaaa aggggcagaa atggttcgcc aagtcttgaa agataatttt
    52741 gacttggaca ttaagtacta cgccttggtt gatttccagg cctttgccac agcgattgac
    52801 acgcttttcc ctgatggggt gacgattgat gctcaatttt caacattgaa tggtcgtccc
    52861 ctaacagaag ctacagtcgg agatgattta tacgctaccg agactgagtc tccaacccaa
    52921 accattaaag tcggaaaaca gcagatgaat ggctcgaccc tgctcaatta tgctcgtttc
    52981 cgtgatgatg atgaggcgga ttacggccgt acaaaaagac agcaacaagt tttaacagca
    53041 attttagagc aaattaaaga tcccactaaa cttttcactg gttctgaagc tcttggaaag
    53101 gtttttgcta tgacctcaac caacgtaccc tatactttcc tcctaacaaa cggtttatct
    53161 gttttggatg gagcaaaaaa tggtattgaa aaattgacga ttccagaact gggtgactgg
    53221 gtagatgcct atgatgttta tggaggcttg ggcttgctgg ttgatcaaaa caaatatcaa
    53281 accaagctcg ctcaaatggg cttgagataa gataatactc aatgaaaatc aaagtgcaaa
    53341 ctaggaagct agccgcaggc tgctaaaaac acagttttga ggttgtagat aagactgacg
    53401 aagtcagtta cctataccta cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa
    53461 gagtataaga cagaaaaatc aaagtttgat agctatttgg atagcagtca ggctttgatt
    53521 ttttacagtc tgcaatagat tttcacccct tgtttgtggt ataatgaaac gatacgatag
    53581 aaagaataat gaacaatatg actgatttaa aagcaattca ggctcgtagt ctggagatgg
    53641 ctgaatattt tgtggccttt tgtaaagaac atgatttgct ctgttatctc tgtggcggag
    53701 gcgctattgg tgcccttcga aacaagggat ttattccttg ggacgacgac ctagactttt
    53761 ttatgcctcg taaagattat gagaaattag cagaattatg gcctcgttat gcagatgaac
    53821 gttatttctt gtcaaagagt cacaaggatt ttgttgatcg taatcttttt attaccattc
    53881 gtgacaagaa aaccacctgt atcaagcctt atcagcagga tttggatttg ccacatggtc
    53941 tggccttgga tgttttgcct ttggattatt atccgaaaaa tccagctgag cggaaaaaac
    54001 aggttcgttg ggccttgatt tattcactct tttgtgcgca aactattcca gaaaagcatg
    54061 gtgctctcat gaaatgggga agtcgcattt tactgggttt gactccaaaa tctctctgtt
    54121 atcgcatctg gaaaaaagct gagaaagaaa tgactaagta tgatttggct gattgtgatg
    54181 gcattacaga attatgctca ggtcctggct acatgagaaa caagtaccca atcacatctt
    54241 ttgaagacaa tcttttcttg ccatttgaag gaacagagat gcctattcca atcggctatg
    54301 atgtctatct cagaactgct tttggggatt atatgacgcc tccaccagca gacaagcagg
    54361 taccgcatca tgatgctgtc atcgctgata tggataagtc ttatacagaa tacaagggag
    54421 aatatggtgg ctaagaaaaa aatcttattt tttatgtggt ctttttctct tggaggtggt
    54481 gcagagaaga ttctatcaac cattgtttca aatctggatc cagaaaagta tgatattgat
    54541 attcttgaaa tggagcactt tgacaaggga tatgaatctg ttccaaagca tgtacgcatt
    54601 ttaaaatccc ttcaagatta tcgccaaacc agatggttac gagctttttt gtggagaatg
    54661 agaatttatt ttccaagact ggctcgtcgt ttgcttgtaa aagatgatta tgatgttgaa
    54721 gtttctttta ccattatgaa tccaccactg ttgttctcta aaagaagaga agtcaagaag
    54781 atatcttgga ttcatggaag tattgaagaa cttcttaagg atagctctaa aagagaatca
    54841 catagaagcc agttggatgc tgcgaataca attgtaggga tttcaaaaaa gaccagcaat
    54901 tctatcaagg aagtttatcc agattatgct tctaaattac agacaatcta caatggatat
    54961 gattttcaga ctattctaga aaaatctcaa gagaagatcg atatcgagat tgctcctcaa
    55021 agtatctgta ctatcggacg gattgaggaa aataagggtt ctgaccgtgt agtggaagtg
    55081 atacgattat tacaccaaga gggaaaaaac tatcatctct attttatcgg ggctggtgat
    55141 atggaagagg aactgaaaaa acgagtcaaa gagtatggga ttgaggacta tgtacatttc
    55201 cttggttatc aaaaaaatcc ttatcagtat ctatctcaga cgaaagttct tttgtctatg
    55261 tctaaacaag aaggttttcc tggagtgtat gtggaggcct tgagtctggg actccctttt
    55321 atctctacga acgttggagg ggctgaggaa ttatcccaag aaggacgatt tggacaaatc
    55381 attgagagca atcaagaggc agctcaggcg attactaatt acatgacttc tgcctcaaac
    55441 tttgatgtcg atgaggctag ccaattcatt caacaattta caattacaaa acaaatcgaa
    55501 caagtagaaa aactattaga ggagtagcat ggaaactgca ttaattagtg tgattgtgcc
    55561 agtctataat gtggcgcagt acctagaaaa atcgatagct tccattcaga agcagaccta
    55621 tcaaaatctg gaaattattc ttgttgatga tggtgcaaca gatgaaagtg gtcgcttgtg
    55681 tgattcaatc gctgaacaag atgacagggt gtcagtgctt cataaaaaga acgaaggatt
    55741 gtcgcaagca cgaaatgatg ggatgaagca ggctcacggg gattatctga tttttattga
    55801 ctcagatgat tatatccatc cagaaatgat tcagagctta tatgagcaat tagttcaaga
    55861 agatgcggat gtttcgagct gtggtgtcat gaatgtctat gctaatgatg aaagcccaca
    55921 gtcagccaat caggatgact attttgtctg tgattctcaa acatttctaa aggaatacct
    55981 cataggtgaa aaaatacctg ggacgatttg caataagcta atcaagagac agattgcaac
    56041 tgccctatcc tttcctaagg ggttgattta cgaagatgcc tattaccatt ttgatttaat
    56101 caagttggcc aagaagtatg tggttaatac taaaccctat tattactatt tccatagagg
    56161 ggatagtatt acgaccaaac cctatgcaga gaaggattta gcctatattg atatctacca
    56221 aaagttttat aatgaagttg tgaaaaacta tcctgacttg aaagaggtcg cttttttcag
    56281 attggcctat gcccacttct ttattctgga taagatgttg ctagatgatc agtataaaca
    56341 gtttgaagcc tattctcaga ttcatcgttt tttaaaaggc catgcctttg ctatttctag
    56401 gaatccaatt ttccgtaagg ggagaagaat tagtgctttg gccctattca taaatatttc
    56461 cttatatcga ttcttattac tgaaaaatat tgaaaaatct aaaaaattac attagaacgg
    56521 gggtgaccaa gtgattgatg ggaaacgatt attatttagt ttgaccatag tcagttatgc
    56581 cttgacgcta gtaagtggaa ttgtgtatct gtttaataat aataatgtta gcttactttc
    56641 tactttattg ttcttgttgg ttagtagctt aattgcttgt tggaatgata tcaagtatta
    56701 cttaatccat tttattttct atttaaccat ttttgtattt ctggtatcaa gaccgaccat
    56761 tgattatttt agggatggtg ctttggatac ctatcatcca atagcctatc gttttgcctt
    56821 tatagttgtc atgatttcga ttctgggctt gaccacagga ggcattctgg ctcgttactt
    56881 catagctagg aagaaaataa aagtagcaaa tataggaaat tctctaaaag aggtttatat
    56941 caagcggtta cgctttgtat cactaggagt ttttcttcta acttatcctt tctatttcat
    57001 tcggttattt gaacggctct tgtatcgttt gcagacttcc tactatgcct actatgcaaa
    57061 ttttgaaagt aaactgcctt attttaccta cattttgtct acctttacgg tctatgcaat
    57121 gtgtatgtat ctggcaacca agccaaagaa attgcaggcc acagcagtgc ttgtctcctt
    57181 tattgcagct aatactattc atttggcaat tgggacacga aatcccttta ttttaagtat
    57241 tttatttgct tttgtttatt actttatgcg ggagcaaact gaaaaaggaa aatggattgg
    57301 gtttaaagaa aagttagcga tttttgtagg ttctcctatt ctcatgttag cgatgggagt
    57361 actcaattat gtacgggata atgtccaagt ttcccataca ggtttctggg atatcttact
    57421 tgactttatc tataaacaag ggactagttt tggtgttctg gctcgaggtt ttctatttaa
    57481 cagtagcctc ccttaccgag atttccgtaa ttttactttt ggtcctgttc ttgattattt
    57541 tgcaaggggg agtttgggag ccattttcgg aggaaaagcc tttgaacata caaccaatag
    57601 tgtggaacta gctattgata gtaatagtta tgcccacaat ctatcctatc ttgtcttgaa
    57661 caaggaatac ttgaaagggc atggtatcgg aagtagttat atcatggagt tgtataccga
    57721 ctatggtatg attggagtct ttctgcttag tttcttactc ggcgtattat ttatagccat
    57781 gctgcaagta gcctatcgct caaggacaat cctatttgct ttatccctac tcatcttgaa
    57841 taatctattc tttatgccaa gaagcagctt ttcagaaagt ttcttcaatt tatttacaat
    57901 gcaattctgg ggaattgttc ttgtgattat atttgtagca aaaatgctta caaaggaaaa
    57961 ctagtatcta ctaaacaaag gagaaaaaaa tcatgtttga acattattca gtagctgatt
    58021 tgtttgcaaa tctttataaa aagcgaaaag caaatatttt agctctcatc gctttatttg
    58081 ctctcattgc tgtaccattt acaattaaag cagttaggaa taaaaacact gtcaaagata
    58141 caacaagtta ttcaacttat cttatctata aaatcactcc tccaaaagag tcggccaaaa
    58201 cgattttgaa tcatcaaatt ggtggttata gtgattttta tgggaaattg attgatggta
    58261 atttgaatgg agcttatctt ttcaatgatg tagaacccag tgagttgaaa aaaattgcca
    58321 gtgaattaga cacgacagaa acaaccttga aaaattcaac gaatgactat tggtggaaaa
    58381 aattgaccgt ctactatatg attgacgatg caggggttgg tgtgaaaatt ttgacatcaa
    58441 gtaaagatgc caatgacttg ttagagaaaa aaattgatgg gttgattgag aaatttaaac
    58501 atgcctatgc aaatgtgaaa attgaaaaac tggaaaccat caactctaaa gaattgaacg
    58561 caaatggtga aacagcgctt ggcttaaatg tgaaaaatct gattcttcgt ttagttgtta
    58621 ttggagtggt ttgtgtgatt ttggttgtga tgggaaatgt gttagtttat ctctttaatc
    58681 caacaatcaa tagagtaggt gatttttctc agtatcaaat tgattttgta acagagatta
    58741 caacaattgc taacctagca gatgttttgt catacaaaaa tactggacag gaattgacca
    58801 tcgttagctc aaataaagct atcctagata aattgaaaca gagtcaagaa gctttaaaag
    58861 gaatgcattt tgtagattta caggatgtat catctctttt ggaaagagat acagtccttc
    58921 ttgttgaaga gtacggagtg actcgttata agaaatttga gcaaagtctt caaattctca
    58981 gaaacttaaa tcgttctatc ctcggtgtag caacctttaa attataagta ttctgatgta
    59041 taaggtcttc aaagtcggct aagagccgat tttgaaggct ttttctatct gtctgcagaa
    59101 attttctttt gataaaatcg taaaaatcta gtataataga tagaactgaa agtatgaggt
    59161 tactagcaat gaaaatgaaa caaattagtg atacaacttt aaaaatcacg atatctttag
    59221 aggatttgat ggatcgtgga atggagattg ctgactttct cgttcctcaa gaaaaaacag
    59281 aagagttctt ttatgctatc ttggatgagc tagagatgcc tgatagcttt ctggatacag
    59341 gcatgttgag cttccgtgtg actccaaaac ctgataaggt agatgtcttt gtgaccaagt
    59401 caaagattga ccaaaatcta gattttgaag acttatcgga tttgccagat atggaagaat
    59461 tggctcaaat gtctccagat gaatttatca aaaccctgga aaaaagcatc gcagacaaaa
    59521 ccaaggatga tatcgaagcc attcaatctc ttgagcaagt tgaagccaag gaagaagagc
    59581 aagagcaggc tgaacaagaa gctgagagta agaaagaacc ttacatctac tacatccttt
    59641 cttttgctaa gttggctgac ttggtagctt ttgccaagac agtgactttt gaaatggaaa
    59701 cttctgaact ctacaaaatg aacgagcgct attatttgac cattttagtg gatattgaaa
    59761 atcatccaag cccatatcca gcttggctgt tggcccgtat gcgcgagttt gcagaggata
    59821 gtgatatcag tcgctcagtc ttacaagagt atggtcaagt cttgatgagt cacgatgcag
    59881 tgctcaatct gcaaaaaatc ggctaatcct ttctggaaag ctattttcag attttaagaa
    59941 gagcgaatcg tctgattcgt tttttctttt tatactgaaa tagtgattta ctataatagg
    60001 aattttcaca aaattctgtt ataatggcta tattagaaaa tttcgaggag ataaagatga
    60061 cagttaaaat tgctttactg ggatttggta ccgttgcaag tggtgtgcct ttcctcctaa
    60121 aggaaaatgg aggaaaaatc aatcaatcag cacattcaga tatcaaagtt gctaaggtat
    60181 tggtcaagga tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac tttaactttg
    60241 taaccaatgt ggatgatatt ttatcagacc aggatattac tatcgtagtg gaattgatgg
    60301 ggcgtattga gcctgctaaa acctttatca ctcgtgcctt ggaagctgga aaacacgttg
    60361 ttactgctaa caaggacctt ttagctgtcc atggcgcaga attgctagaa atcgctcaag
    60421 ctaacaaggt agcactttac tacgaagcag cagttgctgg tgggattcca attcttcgta
    60481 ctttagcaaa ttccttggct tctgataaaa ttacgcgcgt gcttggagta gtcaacggaa
    60541 cttccaactt catggtgacc aagatggtgg aagaaggctg gtcttacgat gatgctcttg
    60601 cggaagcaca acgtctagga tttgcagaaa gcgatccgac gaatgacgta gatgggattg
    60661 atgcagccta caagatggtt attttgagcc aatttgcctt tggcatgaag attgcctttg
    60721 atgatgtagc ccacaaggga atccgcaata tcacaccaga agacgtagct gtagctcaag
    60781 agcttggtta cgtagtgaaa ttggttggtt ctattgagga aacttcttca ggtattgctg
    60841 cagaagtgac tccaaccttc ctacctaaag cgcacccact tgctagtgtg aatggcgtaa
    60901 tgaacgctgt ctttgtagaa tctatcggta ttggtgagtc tatgtactac ggaccaggtg
    60961 ctggtcaaaa accaactgca acaagtgttg tagctgatat tgtccgtatc gttcgtcgtt
    61021 tgaatgatgg tactattggc aaagacttca acgaatatag ccgtgacttg gtcttggcaa
    61081 atcctgaaga tgtcaaagca aactactatt tctcaatctt ggctctagac tcaaaaggtc
    61141 aggtcttgaa gttggctgaa atcttcaatg ctcaagatat ttcctttaag caaatccttc
    61201 aagatggcaa agagggtgac aaggcgcgtg tcgttatcat cacacacaag attaataaag
    61261 cccagcttga aaatgtctca gctgaattga agaaggtttc agaattcgac ctcttgaata
    61321 ccttcaaggt gctaggagaa taagatgaag attattgtac ctgcaaccag tgccaatatc
    61381 gggccaggtt ttgactcggt cggtgtagct gtaaccaagt atcttcaaat tgaggtctgc
    61441 gaagaacgag atgagtggct gattgaacac cagattggca aatggattcc acatgatgag
    61501 cgtaatctct tgctcaaaat cgctttgcaa attgtaccag acttgcaacc aagacgcttg
    61561 aaaatgacca gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc ggttatcgtt
    61621 gctgggattg aactagccaa ccaactgggt caactcaact tatcagacca tgaaaaattg
    61681 cagttagcga ccaagattga agggcatcct gacaatgtgg ctccagccat ttatggtaat
    61741 ctcgttattg caagttctgt tgaagggcaa gtctctgcta tcgtagcaga ctttccagag
    61801 tgtgattttc tagcttacat tccaaactat gaattacgta ctcgcgacag ccgtagtgtc
    61861 ttgcctaaaa aattgtctta taaggaagct gttgctgcaa gttctatcgc caatgtagcg
    61921 gttgctgcct tgttggcagg agacatggtg accgctgggc aagcaatcga gggagacctc
    61981 ttccatgagc gctatcgtca ggacttggta agagaatttg cgatgattaa gcaagtgacc
    62041 aaagaaaatg gggcctatgc aacctacctt tctggtgctg ggccgacagt tatggttctg
    62101 gcttctcatg acaagatgcc aacaattaag gcagaattgg aaaagcaacc tttcaaagga
    62161 aaactgcatg acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa ataaagaata
    62221 gaagatagga tggggaaact cttgaccaga gggtttcata tcctttttgt gaaaagaagt
    62281 ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg ctcaaaacag
    62341 tgttttgagg ttgtggatag aactgacgaa gtcagctcaa gacactgttt tgaggttgca
    62401 gatagaactg acgaagtcag taacatacct acggtaaggt gacgctgacg tggtttgaag
    62461 agattttcga agagtattag ttaaaaacgt gataaaggag aaataaaaat ggcagaaatt
    62521 tatctagcag gtggttgttt ttggggccta gaggaatatt tttcacgcat ttctggagtg
    62581 ctagaaacca gtgttggcta cgctaatggt caagtcgaaa cgaccaatta ccagttgctc
    62641 aaggaaacag accatgcaga aacggtccaa gtgatttacg atgagaagga agtgtcactc
    62701 agagagattt tactttatta tttccgagtt atcgatcctc tatctatcaa tcaacaaggg
    62761 aatgaccgtg gtcgccaata tcgaactggg atttattatc aggatgaagc agatttgcca
    62821 gctatctaca cagtggtgca ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa
    62881 gtggagcaat tacgccacta cattctggct gaagactacc accaagacta tctcaggaag
    62941 aatccttcag gttactgtca tatcgatgtg accgatgctg ataagccatt gattgatgca
    63001 gcaaactatg aaaagcctag tcaagaggtg ttgaaggcca gtctatctga agagtcttat
    63061 cgtgtcacac aagaagctgc tacagaggct ccatttacca atgcctatga ccaaaccttt
    63121 gaagagggga tttatgtaga tattacgaca ggtgagccac tcttttttgc caaggataag
    63181 tttgcttcag gttgtggttg gccaagtttt agccgtccga tttccaaaga gttgattcat
    63241 tattacaagg atctgagcca tggaatggag cgaattgaag ttcgttctcg ttcaggcagt
    63301 gctcacttgg gtcatgtttt cacagatgga ccgcgggagt taggcggcct ccgttactgt
    63361 atcaattctg cttctttacg ctttgtggcc aaggatgaga tggaaaaagc aggatatggc
    63421 tatctattgc cttacttaaa caaataaaac agagagtggg gcttcccact ttcttcattt
    63481 ctagaatatg aatagaaggg atttatgaaa tacctattat cttacttcaa accctacatc
    63541 aaggaatcaa ttttagcccc cttgttcaag ctgttagaag ctgtttttga gctcttggtt
    63601 cccatggtga ttgctgggat tgttgaccaa tctttacctc agggagatca aggtcatctc
    63661 tggatgcaga ttggcctgct ccttatcttt gcagtaattg gcgttttagt ggccttgata
    63721 gctcaatttt actcagcaaa ggcagcagta ggctttgcta aggaattgac aaacgatctt
    63781 tatcgtcata ttctttcctt gcccaaggac agcagagacc gtctgacaac ttctagtttg
    63841 gtcactcgct tgacttcgga tacctaccag attcagactg gtatcaatca attcctgcgt
    63901 ctctttttgc gagcgcccat tatcgttttt ggtgccattt ttatggctta tcgaatctca
    63961 gctgagttga ctttctggtt cttagtcatg gttgccattt tgaccattgt aattgtaggg
    64021 ttatctcgat tggtcaatcc tctctacagt agtctcagaa agaaaacgga ccaactggtt
    64081 caggaaacgc gccagcaatt gcaagggatg cgggttattc gtgcttttgg tcaagaaaaa
    64141 cgagagttac agatttttca aacccttaac caagtttatg ctagattaca agaaaagaca
    64201 ggtttctggt ctagtttatt aacacctctg acctatctga ttgtcaatgg aactcttctc
    64261 gttattatct ggcaaggcta tatttcaatt caaggaggag tgctcagtca aggtgctctc
    64321 attgctctta tcaattacct cttacagatt ttggtggaat tggtcaagct agccatgttg
    64381 atcaattccc tcaaccagtc ctatatctca gccaagcgaa tcgaggaagt ctttgttgag
    64441 gctccagagg atatccattc agagttagaa caaaagcaag ctaccagaga taaggtttta
    64501 caagtccaag aattgacctt tacctatcct gatgcagccc agccttctct gagagacatt
    64561 tcctttgata tgactcaagg acaaattcta ggtatcatcg ggggaactgg ttctggtaaa
    64621 tcaagcttga ttcaactcat acttggactt tatccagtaa acaagggaag aattgacctt
    64681 tatctagatg gattgagtcc tcttaatttg gagcaatgga gatcttggat tgcctatgtg
    64741 ccacaaaagg tcgaactctt taagggaact attcgttcca acttgacttt aggtttaaat
    64801 caagaagtga ctgatcaaga actctggcaa gccttggaaa ttgctcaagc taaggatttt
    64861 gtcagtgata aggaaggact cttggatgct ctagttgaag caggaggtcg aaatttctca
    64921 ggtggacaaa aacaaaggct gtctatcgca cgagcagtct tgcgccaggc tccgtttctc
    64981 atcctagatg atgcgacctc ggcactggat accattaccg agtccaagct cttgaaagct
    65041 attagagaaa attttccaaa catgagctta attttgatct ctcaacgaac ctcgacttta
    65101 cagatggcgg accagattct cctcttggaa aaaggtgagt tgctagctgt tggcaagcac
    65161 gatgacttga tgaaatccag tcaagtctat cgtgaaatca atgcatccca acatggaaag
    65221 gaggactaga atgaaacgac aaactgtaaa ccagacgctc aaacgtttag tcgtagattt
    65281 agcaaaccat cccttcctcc ttttcctagc ctttctagga actattgccc aagttggctt
    65341 atcaatttac ctacctattc tgattgggca ggtcattgac caagtcctag tggctggttc
    65401 atcaccagtt ttttggcaga tttttctcca gatgctcttg gtggtaatag gaaatactct
    65461 ggtacaatgg gccaatcctc tcctctataa tcgtctaatc ttctcttata ccagagattt
    65521 acgagagcga atcatccata aactccatcg tttaccgatt gcctttgtag ataggcaagg
    65581 caatggagag atggtcagtc gtgtaaccac ggatatcgaa cagttggcag ctggcttgac
    65641 catgattttt aaccaatttt tcattggtgt tttgatgatt ttggttagta ttctagccat
    65701 gctccaaatt catctcctca tgactctctt agtcttgctg ttgacgccac tgtccatggt
    65761 gatttcacgc tttattgcca agagatccta tcatctcttc cagaagcaaa cagagacgag
    65821 gggaattcag actcagttga ttgaagaatc gcttagccag cagactataa tccagtcctt
    65881 caatgctcaa acagaattta tccaaagatt gcgtgaggct catgacaact actcaggcta
    65941 ttctcagtca gccatctttt attcttcaac ggtcaatcct tcgactcgct ttgtaaatgc
    66001 actcatttat gcccttttag ctggagtagg agcttatcgt atcatgatgg gttcaacctt
    66061 gactgtcggt cgtttagtga cttttttgaa ctatgttcag caatacacca agccctttaa
    66121 tgatatttct tcagtgctag ctgagttgca aagtgctctg gcttgcgtag agcgtatcta
    66181 tggagtctta gatagccctg aagtggctga aacaggtaag gaagtcttga ccagtgacca
    66241 agttaaggga gctatttcct ttaaacatgt ctcttttggc taccatcctg aaaaaatttt
    66301 gattaaggac ttgtctatcg atattccagc tggtagtaag atagccatcg ttggtccgac
    66361 aggtgctgga aaatcaactc ttatcaatct ccttatgcgt ttttatccca ttagctcggg
    66421 agatatcttg ctggatgggc aatccattta tgattataca cgagcatcat tgagacagca
    66481 gtttggtatg gtgcttcaag aaacctggct cacacaaggg accattcatg ataatattgc
    66541 ctttggcaat cctgaagcca gtcgagagca agtaattgct gctgccaaag cagctaatgc
    66601 agactttttc atccaacagt ttccacaggg atacgatacc aagttggaaa atgctggaga
    66661 atctctctct gtcggccaag ctcagctctt gaccatagcc cgagtctttc tggctattcc
    66721 aaagattctt atcttagacg aggcaacttc ttccattgat acacggacag aagtgctggt
    66781 acaggatgcc tttgcaaaac tcatgaaggg ccgcacaagc ttcatcattg ctcaccgttt
    66841 gtcaaccatt caggatgcgg atttaattct tgtcttagta gatggtgata ttgttgaata
    66901 tggtaaccat caagaactca tggatagaaa gggtaagtat taccaaatgc aaaaagctgc
    66961 ggcttttagt tctgaataag ccattctctt ttgaaagttt atggacgaaa aaagttgcct
    67021 tcgagtgact tttttgttac aatagctaga aaaattgttc actgtaatac tcaatgaaaa
    67081 tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcacagcttt gaggttgtag
    67141 ataagactga cgaagtcagc tcaaaacact gttttgaggt tgcagataga actgacgaag
    67201 tcagtaacca cacctacggc aaagtgaatc tgaagtggtt tgaagagagt acaacttgtc
    67261 ttttaaaaaa ggagtctata atgaaagtct ttcagcatgt aaatatcgtg acttgtgatc
    67321 aaaatttcca tgtttatctt gatggaatct tagcagtcaa ggattctcaa atcgtctatg
    67381 ttggtcaaga taagccagca tttttagaac aagctgagca gattatagac tatcagggag
    67441 cttggattat gcctggtttg gtcaattgtc acacccattc tgcaatgaca ggtctgagag
    67501 ggatccgaga tgacagcaat ctccatgaat ggctcaatga ctatatctgg ccagcagaat
    67561 ctgagtttac tcccgacatg actaccaatg cggtcaaaga agccctgaca gagatgctcc
    67621 agtcaggaac aacaaccttt aacgatatgt ataatcccaa tggtgtggat atccagcaaa
    67681 tttatcaggt ggtgaaaact tccaagatgc gttgttattt ttctccgact ctcttttctt
    67741 cagagacaga aacaactgct gagactataa gcagaactcg atccatcata gacgaaatct
    67801 taaaatataa aaatccaaat ttcaaggtta tggtagcacc tcattctccg tatagctgca
    67861 gtagagactt gctggaagcg agtttggaaa tggcaaaaga gctaaatatt ccgctccatg
    67921 tccatgtggc ggagaccaag gaggagtcag gaattatcct caaacgatac ggcaaacgcc
    67981 cccttgcttt tcttgaagaa ctgggttatt tagatcatcc gtccgtattt gctcacgggg
    68041 tcgaattaaa cgagagagaa attgaacgct tggcatcttc tcaagtggct atcgcccaca
    68101 atcctatcag taacctcaaa ctggcatcag gaattgctcc aattatccag ctccaaaaag
    68161 cgggagtagc agtcggaatt gcgactgact cggttgcttc caataacaat ctagatatgt
    68221 ttgaggaagg aaggactgca gctcttcttc agaagatgaa aagtggggat gccagccagt
    68281 ttccaatcga aacagctctc aaggtactga caatcgaagg ggctaaggcc cttggaatgg
    68341 aaaatcagat aggaagtctg gaagtcggca agcaagcaga ttttctggtc attcaaccac
    68401 aagggaaaat tcatctccaa cctcaggaaa atatgctgtc tcacctggtt tatgcagtta
    68461 aatctagtga tgtagatgat gtttatatcg ccggagaaca ggttgttaag caaggtcaag
    68521 tcctgacagt agaactttaa aagaaaaatc acgaaaaatt ttaaaaaaag tttgcaaaaa
    68581 tcttgcattc tttttttgac tatgctatac ttatatacgg tttaaaaaaa ctgcctaaga
    68641 cagtagggga gctcgactca taaatatcct accgaggaca agacgtatca tgtaaaaaga
    68701 agcgtattgt actttcgtgt ctaggtttgg gcgcgttttt ctttttgaaa aattccccaa
    68761 gcaaaataat tacggaggtg aacacactaa tgagtgaagc aattattgct aaaaaagcgg
    68821 aactagttga cgtagtagct gaaaaaatga aagctgctgc atctatcgtc gttgtagacg
    68881 ctcgtggttt gacagttgag caagatacag ttcttcgtcg tgagcttcgt ggaagcgaag
    68941 ttgagtataa agttattaaa aactcaatct tgcgtcgtgc agctgaaaaa gctggtcttg
    69001 aagatcttgc atctgtattt gttggaccat ctgcagtagc attttctaat gaagatgtta
    69061 tcgcaccagc gaaaatcttg aacaactttt ctaaaaacgc tgaagcactt gaaattaaag
    69121 gtggtgcaat cgaaggcgct gtcgcatcta aagaagagat tcttgcactt gcaactcttc
    69181 caaaccgcga aggacttctt tctatgctcc tttctgtact tcaagcgcca gtgcgcaacg
    69241 ttgctcttgc agtcaaagcg gttgcagaaa gcaaagaaga cgcggcttaa tcttaagcta
    69301 cacagcgtag cctagctacg aaaaaaacta ttataaaatt taaaacttat ttggaggaaa
    69361 taacaatggc attgaacatt gaaaacatta ttgctgaaat taaagaagct tcaatccttg
    69421 aattgaacga ccttgtaaaa gctatcgaag aagaatttgg tgtaactgca gctgctcctg
    69481 tagctgttgc tgcagctgat gcagctgatg ctggtgctgc taaagattca ttcgacgttg
    69541 aattgacatc tgcaggcgac aaaaaagttg gcgttatcaa agttgtacgt gaaattactg
    69601 gtcttggtct taaagaagct aaagaacttg ttgacggtgc accagcactt gttaaagaag
    69661 gcgttgcaac tgcagaagct gaagaaatca aagctaaatt ggaagaagct ggagcttcag
    69721 ttactcttaa ataagaggca tataccaatt agaattcgga atactatagt atcagccttt
    69781 tagaatcgtg aacacatttt agaaactgat aaatttattt agtttcctac caaaaaccct
    69841 accaaaaatt ctttggcagg gtttttcttt ttaataaatt ttcggaggta gacactagat
    69901 tgtctacccc tttttttgtg tttcggaggt aattatgcaa aaggaaggac agtcatcacg
    69961 tcaattcgtg aagagtcatc tcatgactat catgggagtt gatattgtaa aagcaactca
    70021 gttagttgat gaaatggaac aagtaggttt aatccgattt gatgaatttg gaaatgttgg
    70081 aattttagtc ttggagggac aatcatgaag cgtattaccg ctagtcaata tcagacatca
    70141 gagcgttatt ataagctccc caaactttta tttgagagtg aacggtataa gaatatgaaa
    70201 ctggaggtta aggtggttta ctcagtttta aaagatcggt tggagttgtc tttgagcaaa
    70261 gcttggattg atgaggatgg ggctatttat ttgatttatt ccaattcaaa tttgatggca
    70321 cttttaggct gttcaaagtc aaaattactc tccatgtgag tttgaggtga catttttaga
    70381 tgattaccat aaaaaacata attacccact attttacgaa tcctatcttc aaaacgttat
    70441 ggaattcctt gaaagtcaag acataaagaa tggggttgat gcctttgtag atgatcatca
    70501 aaatctcgtt tttgttttat atggacaagg ctatcgagcc gagggaaaag agggaatact
    70561 tacaacccaa gtaactgtaa aagcttatga tgaagacaag aaaccgatta acttcgcaaa
    70621 tttattagat tccttaatcg tctcagaata tcaaatggaa ccgaatcttt gggaggtctc
    70681 ccatgattga tctctatcta agtaaaaata gacaaagaaa tcaacttctt ttagatttct
    70741 tccaaaacta tggcatagag gtatcttgtc attcagtttc tgaaatgaca aaggacaaat
    70801 taattgagat gatgagctat tcttcagatt gttttgagtt tttatctccc aatttattac
    70861 gatttaagaa ccgtgacaat ctaagattaa cggatttcgt tgagataatt ttaaaaaatc
    70921 ctgaactaac catcagactt cctcttgcgg tttcaaataa gcgagtttat ccaagtctga
    70981 atttggaaga ggctagagct ctattgccaa gagatacgaa acaattgatt tacatggcgc
    71041 aaacacatta tttatctaac taaaggagaa gcaatatggc tgaacgattt tgggagaact
    71101 tgtccattat tttggctgaa agaaatatca gctgtattga gttaaccaga aaaatgtttg
    71161 cgggagagtt tcactatcca agtgaattga atcgtttgta tcaaaagatt cgccactaga
    71221 agatggaaca acgaatgcct caaagttcat gggtagaaag gattgtgcag gtattagatt
    71281 tagattatga agatttattt cggaggtaac tatgaaaaga ataattccag tttatatatt
    71341 ccaacaagta aatgtcctat tggtatctct atacttattg aaatttcttt gtatcggtga
    71401 gttaactata ctacagattc tctatggttc gtcactcatt tcttttttat ggatgtatgg
    71461 ccaacgcaaa caagctcata aggtaaatat gaaatctagg atgaaatggc ttggtattgg
    71521 attcgttagc ctactgatta taagtctatg ttttagtcta atccatgcgc aagaaagcac
    71581 gaatcaagca aacttaattg ggcttcaaca tcaagttcct tggttttcat ttttattgtt
    71641 tttaatcaat gcgagtatgg ttgaagaatt tctgtataga gaaattttat ggaacttggt
    71701 tagaaaatta gatattcgag ttgcgttgac aagtgtttta tttgccttag cacatcatcc
    71761 aggaaccatt atagcttggt gtttgtatgt atcgctgggg atgtttttag ggcttgtacg
    71821 ctacaaattg gacttatggg gcagtatggg gctacatttg gtgtggaacc tactagttta
    71881 tagtttgctg cttttttaaa ataggtgacc tgatttatct cataaatctg tagtttttat
    71941 gggattctct tttaatttag aaataaaata tattacctat ataaaataaa gaaactagaa
    72001 atattttttc aattttttta gaaataaaat attatataga aattccgaaa agaaataata
    72061 aaaccaaaat aaaatattac taaaatattt taatatgaac atattattag tgataaacgt
    72121 tcttacaata catataatta cgataaaaag aataaaaact attatatttt tatgtttgtt
    72181 gttctgttat gaaacgaaag aaattggaaa ctagtttcta atttatgaaa aatattgaaa
    72241 aaaaaaaaaa aagtgttaaa ataaagaatg taaacgttta cttcaactaa ggagctcata
    72301 tgttactgca aaaagaacta attccaatga tagaagctaa cttaccaaat atggcatatg
    72361 ctgaaaaaga cattgctaaa ttcttcttaa aacagcaacc tctgaatgat tattcatcta
    72421 aagcattgtg cgaatacctt aatgtatcca aagcaacatt gactcgattt gcgaaaaaat
    72481 gtggttttaa aggttttaga caattcattt tcaaatacca agagatgatt catgagaaag
    72541 aaaagttggc attatataca gaggcaacag aaaaagtttt atccgactat gaggaaatgt
    72601 tgagaaaaac ttacacggtt cttgatgaag ttcaacttga gcgtattgct gagatgatag
    72661 aaactgctga gcgtgtatat ctctacggta aaggaagttc tgttcttgct ttacaagaaa
    72721 tgaagatgag atttatgcgt ctcggagtga ttggtgaagt attatcagac gaggatatga
    72781 ttttgtggag tagcttacta cttaatgaaa attgccttgt cattggagca tccatttcag
    72841 gtcaaactga tattgtacta gaaggtctac aaaaagctgc agataaaggc gctaaaacag
    72901 ttttaatgac tacaagaaaa tttgacgaag aagattgttt ctttgatgaa ctattgttat
    72961 tagcttcgac cgatcatctc tcgtatggca atcgcatatc acctcagttt ccaatacttt
    73021 taattacaga ctgcttattc tctaattatc tggaaagtcc agagagacaa tattattaca
    73081 atcaaactat tatccataag gaggaataat aaaatgagcc aaatttggac taaagaaaaa
    73141 tttataagcc aagttcaagg tggagtcatt gtttcttgtc aagctttacc tggtgaagcc
    73201 ctttataatg aagaatttag cttgatgcct tttatggcta aagcagcttt agaggcagga
    73261 gcagtgggca ttcgcgcaaa ttctgtgcgt gatattaaag caattcagaa agtagtagat
    73321 ttaccaataa ttggaattat caaaagggat tatccacctc aagaaccata tattactgct
    73381 acgatgaaag aagtagatga acttgtagaa tgcggaacaa cagtcattgc atttgatgca
    73441 actttaagac caagatatga tggcttagtt gtcagtgaat ttatcaaaaa aataaaagaa
    73501 aaatatccga atcaattgct gatggcggat gtaagtaatt tagatgaagg tctctatgca
    73561 tttaaatcag gcgttgattt tgttggtaca acattatcag gttacacaag tacaagtgta
    73621 caatcagatg agcctgattt tgaactaatg aaaaaattgg ctgattttaa tattccggta
    73681 attgccgaag gaaaaattca ttatccagaa caattaaaaa aagcttatag tttaggtgtt
    73741 accagtgtag tcattggtgg agcgattaca cgtccaaaag aaattgctca gcgatttatt
    73801 aatgtcatca aataaaaaac aatataaagg agaaatctga tgaaagattt aactaaatac
    73861 aaaggcgtta tccctgcatt ttatgcttgc tatgatgaaa atggtgaaat tagccaagat
    73921 cgtgtaaaat ctctggtaca atatttcatt gacaaaggtg taaaaggtat ctatgtaaat
    73981 ggttcttcag gtgaatgtat ttaccaaagt gtagaagatc gtaaacaaat tattgaagct
    74041 gttatggaag ttgctaaagg taaattaaca gttatcaacc atattgcatg taataacacg
    74101 aaagatagta tcgaattggc aaaacattca gaaagtgttg gagtcgatgc tattgcagct
    74161 atcccaccta tttatttcaa attgccagag tattcaatcg cagcatattg gaatgcaatg
    74221 agtgaagctg cgtcaaatac agattttatt atctataata ttccacaatt ggcaggggtt
    74281 gcgttgactg gtagtttgta tgcaacaatg cgtcaaaatc ctcgtgtgat tggagttaaa
    74341 aattcttcta tgcctgtaca agatattcaa atgtttgtag ctgcaggtgg agaagattac
    74401 attgtattca atggtccaga tgaacaatat cttggtggtc gcttgatggg agcagaagct
    74461 ggtattggtg gtacttatgg cgttatgcca gatttgttct tgaaattgga aagtttgatt
    74521 caagaacgag atttagatac agctaaaaaa cttcaatatg ctatcaatga agttatctat
    74581 aagatgatat caggtaaggc aaatatgtat gctgtagcaa aagaagtttt gcgtctaaat
    74641 gaaaaacttg atttaggttc tgttcgtcaa cctttagaag cattggcaga aggtgacttg
    74701 gaagttgcaa aacaagcagc agaacttatt caacaagcac gaaaagaatt tttataataa
    74761 ataaaatcaa tatggagata tgttatgggc acaacaggat ttacaataat tgacttaatt
    74821 atcttgattg tttatttact tgcggtgttg gttgcaggta tctatttctc taaaaaagag
    74881 atgaaaggaa aagagttctt taaaggagat ggttcggttc cttggtatgt tacttcggta
    74941 tccatttttg ccacaatgct cagtccgatt tccttcttgg gactcgctgg tagctcttat
    75001 gcaggtagct ggattttatg gtttgctcaa ttagggatgg tagtagctat tccactgaca
    75061 attcgtttta tcttacctat ctttgcacgg atagacatcg atacggcata tgattacttg
    75121 gataaacgtt ttaattctaa agcacttcgt attatttcag cactcttgtt tattatttat
    75181 caattgggac gtatgtctat cattatgtac ctcccatcag ctggtttatc agtattgaca
    75241 ggaattgaca tcaatatttt gattattttg atgggtgtag ttgcaattgt ttattcttat
    75301 actggtggtc taaaatccgt attatggaca gactttattc aaggtgtgat tctgattagt
    75361 ggtgtcgttt tagctttatt tgtactgatt gctaatatta aaggtggctt tggtgcagta
    75421 gcagaaacat tagcaaacgg gaaattcctt gctgcaaatg aaaaactttt cgatcctaac
    75481 ttgctttcaa actccatctt tttaattgtg atgggttcag gctttacaat cttgtcttcc
    75541 tatgcttcat ctcaagattt ggttcaacgt tttactacaa cacaaaatat taagaaactt
    75601 aataagatgt tgttcacaaa cggtgttttg tcacttgcaa ctgcaacagt cttttacttg
    75661 attggtacag gcttgtacgt attctatcaa gtacaaaatg cagatagtgc agctagcaat
    75721 atccctcaag accaaatatt tatgtacttt attgcatacc agttaccagt aggtatcaca
    75781 ggtttgatct tggcagcgat ttatgcagca tctcaatcaa ctatttcaac aggtttgaac
    75841 tctgttgcaa cttcatggac attggatatt caagatgtca tttctaaaaa tatgtcagac
    75901 aatcgtcgta cgaaaattgc acaattcgta tctctagcag taggtttatt ctcaattggt
    75961 gtttccattg tcatggctca ctcagatatt aaatctgcat acgaatggtt caatagtttc
    76021 atgggacttg tacttggtct acttggtggt gtatttattc ttggatttgt ttctaaaaaa
    76081 gcaaataaac aaggtgctta tgcagcgctg attgtatcaa ccatcgtcat ggtatttatt
    76141 aaatacttcc ttcctccaac agctgttagc tactgggcat attcattgat ttcaatctct
    76201 gtatcagtag tttcaggtta tattgtatct gttcttactg gaaataaagt atctgcacct
    76261 aaatatacaa cgattcatga tattacagaa attaaagcgg attcaagttg ggaagttcgt
    76321 cactaataga aaagagaaaa taatatgatt atttctgaac aatctgattt taaacgatat
    76381 gcttctgtca ataaatattt ttcaaaagtc tgtgattttt tagaaaatac aaatttgaca
    76441 gatttagttg atggaaaaat tgatattgac ggggaaaatg tttttgcaaa ttgtatgact
    76501 tatctagctg atggagttcc aggagatatt tttgaaactc ataagaaata tttagatatt
    76561 catattgttg ttgaaaatac tgaaaaaatg gcagtaactt ccccagttcg tgcccagtca
    76621 cgtgtacctt ttagcgaaga gaaggatatt gctttttacg atagcaaaga ctatcaaatt
    76681 gttgaattgc ttccagggaa tatgttggtg acttttgaag aagatcttca ccaaccaaag
    76741 attcattgca atgatgaaac tgttaaaaaa cttgttatca aagttttaaa cgaggaaaaa
    76801 taaaatgaaa aaaaatatta aacaatatgt aaccttaggt actgtagtgg tattatcagc
    76861 atttgttgct aactcagttg cagctcagga gactgaaact tctgaagtat caacaccaaa
    76921 gttggtgcaa cctgttgcac caacgactcc gatttcggaa gtacaaccta catcggataa
    76981 ctcttcggaa gttactgtac aacctcgaac agttgaaact actgttaagg atccatcttc
    77041 tacagcggaa gaaactcctg tcttagaaaa aaataatgtt actttaacag ggggcggaga
    77101 aaatgttact aaagagttaa aggataaatt tactagcggt gactttactg tagtgattaa
    77161 gtacaatcag tcaagtgaga aaggcttaca agctctgttt ggaatatcta attccaaacc
    77221 cggtcaacaa aatagttatg tagatgtgtt ccttagagac aatggtgagt tggggatgga
    77281 agcgcgtgat acttcttcca ataaaaataa cctagtatcc agacctgctt cagtttgggg
    77341 taagtacaaa caagaggctg tgactaacac tgttgcagta gtagcagatt cagtcaaaaa
    77401 aacatattct ttatacgcaa atggtacaaa agtagtagaa aagaaagtgg ataatttcct
    77461 aaacatcaag gatattaaag gtattgatta ctatatgctt gggggagtga aacgtgcagg
    77521 aaaaacggcg tttggtttta acggaacact agaaaatatc aaattcttta atagtgcatt
    77581 ggatgaagaa actgttaaaa agatgacaac aaacgctgtt actggacatt taatttatac
    77641 ggctaatgat acaacaggtt ctaactattt ccgtattcca gttctgtata cttttagcaa
    77701 tggtcgggta ttttcaagca ttgacgctcg ttacggtgga actcatgatt tcttgaataa
    77761 aattaatatt gctacaagtt atagtgatga taatggtaag acatggacta aaccaaaatt
    77821 aacattggca ttcgatgatt ttgcgccagt accattagaa tggcctcgtg aagttggtgg
    77881 acgtgactta caaatcagcg gtggtgcaac ctatattgac tctgttattg ttgaaaaaaa
    77941 gaacaaacaa gtactcatgt ttgctgatgt gatgcctgct ggagtaagtt ttagagaagc
    78001 aactagaaaa gattcaggtt ataaacaaat tgatggtaat tattacctta aattaaggaa
    78061 acaaggtgat actgattaca attatactat tcgtgagaat ggtactgtat acgacgatcg
    78121 taccaacaga ccaactgaat tttcagtaga taaaaatttc ggtattaaac aaaatggtaa
    78181 ttatttgacg gtagagcaat attcggtttc atttgaaaat aataaaaaga ctgaatatcg
    78241 taatgggact aaagtccata tgaatatctt ctacaaagat gcattgttta aagtagtgcc
    78301 aacgaactat attgcatata tttctagcaa tgatcatggc gaatcttggt ctgcaccaac
    78361 tttattacct cctataatgg gacttaatcg gaatgcgcca tatttaggtc ctggacgtgg
    78421 aatcattgaa agctcaactg gacgtattct tattccgtct tacactggta aagagtctgc
    78481 gttcatttat agtgacgata atggagcatc ttggaaagtt aaagtagtgc cacttccttc
    78541 tagttggtca gcagaagcac aatttgtaga attgagtcca ggagtaattc aagcatatat
    78601 gcgtacaaat aatggtaaaa ttgcatattt aacaagtaaa gacgcaggta ctacttggag
    78661 tgcaccggaa tatttgaaat ttgtttcaaa tccaagttat ggaacacaat tatcaatcat
    78721 caattatagc caattgattg atggtaaaaa ggctgtcatt ttaagtactc caaactccac
    78781 aaatggtcgt aaacacggac aaatttggat tggtctaatt aatgatgata atacaattga
    78841 ttggcgttat catcacgacg ttgattatag taactatgga tactcatatt caacattgac
    78901 agagttacca aatcatgaaa ttggattgat gtttgaaaaa tttgattcat ggtctcgtaa
    78961 tgaacttcat atgaaaaatg ttgtaccata tataacattt aagattgaag atctgaaaaa
    79021 gaattaaagc tgaaatttga aaatatataa aaagaggata aaaattatgg taaattacgg
    79081 tattgttgga gctggatatt ttggagctga tttagctcgc tcaatgaaca aaattgaaga
    79141 tgcaaaagtg gttgcggtat ttgacccaaa tcatggagaa gaagttgctc aagagttggg
    79201 atcagatgtt tgtgcaagtt tagatgaact tgtagcacgt gaagatattg attgtgtgat
    79261 cgtagcttca cctagctacc ttcaccgtga accagttgtg aaagctgctc aacatggcaa
    79321 acacgtattt tgtgaaaagc caattgcatt gtcttatgaa gattgtaaag ccatggttga
    79381 cgcatgtaaa gaaaataatg tcatctttat ggctggtcac atcatgaact tctttaacgg
    79441 tgtacaccat gctaaagaat tgattactca aggtaaaatc ggtaaagttc tttattgcca
    79501 tgctgctcgt acaggttggg aagaacaaca accaactgta tcatggaaga aacttcgttc
    79561 tcaatctgga ggacatttgt accaccatat tcatgaatta gattgcattc agtttatcat
    79621 gggaggactt cctgaaaaag cgacaatggt aggaggcaat gtatatcata aaggtgaaaa
    79681 ctttggtgat gaagatgata tgctcattgt aaacttagaa tactctgatg atcgttatgc
    79741 tgttttggaa tatggtaatg ctttccgttg gggtgaacac tacgtcttga ttcaaggaac
    79801 tgaaggagct atcaaacttg acttgttcaa tactggcggt actcttcgtg ttaaaggtga
    79861 aggagaatca cacttcttag ttcatgaaac tcaagaggaa gatgatgatc gtacagctat
    79921 ctataccggt cgtggtatgg atggagcaat tgcgtacggt aaaccaggag tacgttgccc
    79981 attatggttg caaacatgta ttgataaaga aatggaatat ctacatgaca tcattaaagg
    80041 tggagaaatt acagaagaat ttgaaaaact tctcaatggt gtagctgctt tagaatcaat
    80101 cgctaccgct gatgcatgta ctttatcagt taaagaagat cgaaaagtaa gtctttcaga
    80161 aatcacaaat gcttaacttt tgtaaaacag aatagtaaat tcttgtcatt atataatttc
    80221 taaagttctg tgatacaact cattgaataa agaaatagag atgggactgg gataatgccc
    80281 agtcccattt tttatcaaaa agtaatgaga tcaaaaatgt gggagtgttg aaatgaagat
    80341 tataggtatc gatattggcg gaacaacaat taaggcagat ttatacgatg agtttggaac
    80401 gagtttgaat catttcaaag agatagaaac aattattgac tatgatttgg gaacgaatca
    80461 gatattaaat caggtctgtg atttaattgg tgagtatact ttaaatcatt caattgatgg
    80521 tgttgggatt tccactgctg gagttgttaa tgctaatact ggagaaatca tctatgcagg
    80581 ctatacaata ccagggtata tcggagtaaa ctttactgcc gaaatagaaa aacgttttgg
    80641 gttgtatact tttgttgaaa atgatgttaa ttgtgctgca ttaggtgaat tgtggaaggg
    80701 acaagccaaa gataagaaaa atgtagtaat ggttactatt ggaacaggta taggaggcag
    80761 tattattgtc aacggacaaa ttgttaacgg atttaactat actgctggtg aagtaggtta
    80821 tattcctgta ggtaattcgg attggcaaag taaagcctca acaaccgcat tgattcattt
    80881 atatcaaaaa aagagcttga aaactaatca aactggacgt actttcttca ctgatttaag
    80941 atctggagat aaagttgctg aagaaacttt tgaaattttt gtagaaaatc taacaaaagg
    81001 tttattaacg atttcttatc tacttaatcc agaaattctc atattaggag gtgggattct
    81061 ggatagtaag gatattttgt tacctgaaat tcaaagttct ttagctaaaa atgcaatgga
    81121 taataggttt ttacctaaaa atcttgtggc agctacatta ggaaatgaag ctggtcgtat
    81181 aggagctgta aaaaatttct tagatagaat ttctaataaa tagtatgtaa gataaggagg
    81241 tgtcacaatg actaactctg tattttcgac aatgcaagat attgagaatg ttgcaaccga
    81301 tattataaaa tcatatgata atgagattta tacttataaa gctgtttccc aagaagaatt
    81361 ggaaaaacta gaaaaaagtt atgatgaaaa aagtcacgaa gaattagttt caatagaaag
    81421 caatttagaa atgaaacaac agaaccttat tgatgaggtt aataaaacaa tcaaggaaaa
    81481 tgatgcaaat attcagtata tttcatcaag taggagagga gaatttgtag aaaaaattat
    81541 tggtagggtg gtagaaaaat atggccatta gtcagatgaa aagaatctct ctactatttt
    81601 ctaaaagtag tcttgatgat gttttaaaaa ctattcaaga actagagtca gtgcagttcc
    81661 gtgatttaaa ggttcaggat aactggtcag aagctctaga aaaagatgaa gttgtatttc
    81721 caactattca aatttttcat acttctaatt ccaatcatgg ggttattgag ggaaatgatg
    81781 ccttgactta tttgatgaat caacaacaac atttagaagc aactgtagag aaattacaag
    81841 aatacctacc gaaagaaaac acgtttaaat tattgcagca acctccgata actacctctt
    81901 atgaagaatt agagaaattt ggtaaagcta atgttgctga gggtgttctt aaaaaagtga
    81961 atcatcaaat taacagagtt catgaattag aaagacacat tcaaagtaat aatgaggaaa
    82021 tagagcgatt aataaagtgg gaaaaattag aaattgttcc tgcgaattta gaacaatttt
    82081 ctttctgtaa aggaaaagtc ggaacaattc caaggactga agataatcgc ttatacaata
    82141 gtcttttaga aaacaatatt gaagttcaag aaatattttc taatgataga gagtacggtg
    82201 ttgttgtttt ctatcagtct agttactcta tagattttga tgaatactta tttgaaccat
    82261 ttgattattc tagaaaggaa ttaccgaagc agcgagtagt agatttagat caagaaaaca
    82321 tgcagttaat aactgaaaaa gagaatatta tcgcatcgtt gcaagattca aagaaatatt
    82381 tgatagattt acaatggcaa atagactata ttttatctat ctatgctcgt caaatctcta
    82441 agaataactt tttgtgcact ccgcatctag ttgcattaga aggatggata gaagaaactc
    82501 gtattttata ttttataaaa gttatggatg agcattttgg acattctatt tatatttatg
    82561 aatcggaaac attgacggat aatcaagatg aaatacctat caaattaacg aatcattctt
    82621 taattgaacc atttgaatta ttgacagaaa tgtatgctct gcccaaatat tatgagaaag
    82681 atcctacacc tgtattagca ccattttact ttacattttt tggaatgatg gttgctgatt
    82741 taggctatgg tttactattg tttttaggaa caatgttagc attaaaaatt tttcatctac
    82801 cttcagcaac taagagattt ttaaaattct ttaatatatt aggggtagcc gttgcaattt
    82861 ggggtggaat ctatggctca ttttttggat atgagttgcc atttcatctg atatctacaa
    82921 cctctgatgt catgactata ttagtagtgt cagttgtgtt tgggtttatt acagtatttg
    82981 caggtttgtt agcttcagga ctacaaaaag taagaatgaa gaaatatgca gaagcatata
    83041 attcaggatt tgcgtggtgt gttattctgc ttggcttgtt atttattgct gttggaatgt
    83101 tgatgcctga tatgagaccg ttatttgtat tagggaaatg ggtatctatt tttaatgctg
    83161 tggggatttt gattgtttct attattcaaa ccaaaagctt gtcaggtatt ggagcaggat
    83221 tgtttaatct atataacatt tcatcttata taggtgattt agttagtttc actcgattga
    83281 tggcattagg attatctgga gcaagtatag catcagcttt caatttaatt gttggtttgt
    83341 ttccgggaat attggctaaa ctgacaattg gattagtatt attcattctt ttacatgcga
    83401 tcaatatttt tctatcgtta ctatcaggat atgttcatgg agcacgtctg atatttgttg
    83461 aattttttgg taagttttat gagggtggag gaaaaccatt tcaacctttg aaggcttctg
    83521 agaaatatat taaggttatt acaaagaatt aatggaggat atatataatg gaacatttag
    83581 caacttattt ttcaacctat ggaggagctt tcttcgctgc attgggaatt gtattggcgg
    83641 ttggattaag cggtatgggg tctgcttatg gagttggtaa ggctgggcaa tctgccgcag
    83701 ctttactgaa agaacagcct gaaaagtttg cctcagcttt gatattgcaa ttattgcccg
    83761 gaacacaagg attatatggt tttgttattg gaattttaat ttggttgcaa ttaactccag
    83821 aacttccttt agaaaaaggc gttgcttatt tctttgtagc tcttccaatt gctattgtag
    83881 gatacttttc agctaagcat caaggaaatg tagcagtagc gggaatgcaa atcttggcta
    83941 aaagaccaaa agaattcatg aagggagcaa ttttagctgc catggtagaa acctatgcaa
    84001 ttcttgcttt tgtcgtatca ttcattttga cccttcgtgt ataagaaata aatttgcaat
    84061 tcaaaggagg tgtctaaatg agcaatttag aaaacttacg agagtctgtt attgaacaag
    84121 ctcatgaaaa agggcgtatg aaattattgg attccaaaaa gaagattgat gatgaatttg
    84181 aaatgcaaaa gtcgctcatt ataaagaaaa aagaagctga acatgaacga aagttaaaag
    84241 aattgcaaca gaaatatcaa ataatttttc aacaattaaa aaataaggaa cgccaatcaa
    84301 cgttagtatc aaaacagaaa atattaaaag aactttttca atctgcttta ctagaaatgg
    84361 aatcttggag tgcagataaa gaaatggagt tcatctatcg aattctggaa cgatattcac
    84421 aacaagaggt catagtaacc tttggggaac ggactttagc taaattcaat ttggaacaat
    84481 tagagaaatt gaaattctct tttccaaatt atttatttag tgaacaacct atctcaaatg
    84541 aatcaggctt acttatttca ataggtaaaa ttgatgataa ctatttgtat aaaacattaa
    84601 ttggatcgat ttctaaggaa gaaagttcaa gtatcgcaaa tcaaattttt atcaattaag
    84661 gatgaaattg gttaatcctt cttagaaatt tggagtattc caataaaatt agaaaggtat
    84721 tttatggata ctaatctttt ttcaaaaata aatacgacga tttcggtaaa agaaaacgat
    84781 tttattacag aagaaaaatt tcaaaaaatt atacaatcca aagatacgga gacattggca
    84841 tttatcttag aatcaactcc ctatcattta tcgattgaca tcttagaaga tcctagtcag
    84901 acagagattt cgctaatgac aaaattagtc aatgattata gatgggccta tgctgaaagt
    84961 ccgtctgata taattgtgac tttatttgct ttacgatatg tttatcataa tatcaaagtt
    85021 ttattaaaat ctaaggcggc aattaagaaa gatttttcta aattattaat tccaataggg
    85081 atttttgata tagaaagttt aaaacattta gtttcttcct tacattcaga tacacttcct
    85141 gattttatgg ttcgtgaagt agaatcaatt tggaatgagt atgaaacttt taataatatt
    85201 cgtgtacttg atgtcggagc tgatctagca tattttaaac atctgaaact tttatctaat
    85261 gagttagatg aggtactgtc tcaggttatt gtcgaaatga ttgactttta taatattatt
    85321 actgtaaaac gtggtttatc tcaaaataag agtcatgggg atattttaca attactttca
    85381 gatgaaggaa gtatttctgc taaagaattt atatacattg tagaaaatca agaaatattt
    85441 gtgtggttca ataaaataaa tccaagctta gattcaatct tttcaactta tgaattgaag
    85501 atgcaggacg caacaatttc atcttctgag ttagaatttt tatgtgattt actattgtat
    85561 aaaactttag atcaaggaag gtacaatgta gaggggccgt tagttcttgc tagatattta
    85621 ttgggatgtg agtttgaagt aaagaatctc agaatgatca tatcagctct tcaaaataca
    85681 attccctttg aatcaataaa agaaaggata cgcccacatt atggaagcta ataagtataa
    85741 aattggcata attggtagcc gtgatattat tttaccattt agcatgattg ggtttgatat
    85801 atttcctgcc taccaagaac aagaagctat aaatacacta agaaaattag ctcaatctga
    85861 ttatggtgtc atttatatca ctgaagacat tgcttcaatg atattagata caattcgcca
    85921 ttatgattcc caagttgtgc ctgctattat tttattaccg actcataaac aaggtttaaa
    85981 tttaggatta aaacgtatag aggataatgt agagaaagca gtaggacaca atattttata
    86041 ataatgtaca aaattgtctg taatattatt ctataatttt tggacttagt aaggagaata
    86101 actttgactc aagggaagat tataaaagta tcgggacctc tagttattgc atcaggtatg
    86161 caggaggcta atattcaaga tatttgccgt gtaggtaagc tagggttaat cggtgaaatt
    86221 attgaaatga gaagagatca ggcatctatc caagtctatg aagaaacatc tggtcttggt
    86281 ccgggagaac ctgttgttac aactggagaa cctctctcgg ttgaattagg gccaggattg
    86341 atttctcaaa tgtttgatgg catacaacgc ccattagatc gatttaaatt ggctactcat
    86401 aatgattttc tagttcgtgg ggtagaagtt ccaagtttgg atagagatat taagtggcat
    86461 tttgattcca ctatagcaat tggtcaaaaa gtgagtacgg gtgatattct tggaactgtc
    86521 aaggaaaccg aggtagttaa tcataaaatt atggttcctt atggagtatc tggagaagtc
    86581 gtttctattg catctggcga ttttacaatt gatgaagttg tatatgaaat aaaaaaattg
    86641 gacggtagtt tctataaagg aacgcttatg caaaaatggc ctgtccgcaa ggcgcgtcct
    86701 gtttctaaac gtttaattcc agaagaacca ttaatcacag gtcaacgagt tattgatgca
    86761 ttctttccag taaccaaagg gggagctgca gcagttcctg gaccgtttgg agcaggaaag
    86821 acagttgtac aacaccaagt agctaaattt gccaatgttg atattgttat ttatgtcggt
    86881 tgtggagaac gtggaaatga aatgacggat gtactgaatg agtttcctga gttgattgac
    86941 cctaataccg gacaatcaat tatgcaacgg acagttctga ttgctaatac ttcaaatatg
    87001 cctgttgctg ctcgtgaggc ttcaatttat acaggaatta ccatggctga gtattttcgt
    87061 gatatgggct actctgtcgc cattatggct gattcaactt cacgttgggc agaagcgcta
    87121 cgtgaaatgt caggacgtct agaagaaatg cctggtgatg agggttatcc tgcttatctg
    87181 ggaagtcgta tcgctgaata ttatgaaaga gcaggacgtt ctcaggttct agggcttcca
    87241 gaacgtgaag gaacgattac tgctattgga gctgtatcgc cacctggtgg agatatttca
    87301 gaaccagtta ctcaaaacac tttacggatt gtgaaagttt tttgggggct tgatgctccg
    87361 ttggcacagc gacgtcattt tcctgcaatt aactggctta catcttattc actatataaa
    87421 gacagtgtgg gcacttatat agatggtaaa gagaagacag attggaatag taaaataact
    87481 cgtgcgatga actacttaca acgggaatct agtttagagg aaattgttcg tcttgttgga
    87541 attgattctc tgtctgataa tgaacgacta acgatggaaa ttgctaaaca aattcgagaa
    87601 gattatttgc aacagaacgc ttttgattcg gtagatacat tcacttcgtt tgcaaaacaa
    87661 gaagcaatgc taagtaatat tctcactttt gctgatcagg caaatcatgc tttagagttg
    87721 ggttcttact ttacagagat tatggaaggt accgtggcag ttcgagaccg tatggcgaga
    87781 agtaaatatg tttcagaaga tagattagat gaaatcaaaa ttatatcaaa tgagattaca
    87841 catcaaattc atttgatatt agaaacagga ggtctataaa tgagtgttat aaaagaatac
    87901 agaactgcta gtgaagttgt tgggcctctt atgattgttg aacaagtaaa taatgtgtct
    87961 tacaatgagt tagttgaaat tcaacttcat aatggagaaa ttcgtcgtgg acaagtttta
    88021 gagatccacg aagataaagc aatggttcag ctttttgaag gatctagtgg aataaattta
    88081 gaaaagtcta aaattcgttt tgctggtcat gcattagaat tggctgtatc tgaggatatg
    88141 gttggtcgta tttttaatgg gatgggaaaa ccaattgatg gtggaccaga tttaattcca
    88201 gagaaatatt tagatattga tggtcaagct attaatcctg tatctagaga ttatccagat
    88261 gaatttattc agacagggat ctcctctatt gatcatttga atactcttgt acgtggtcaa
    88321 aaattaccag tattttcagg ttcgggctta cctcataatg aattagctgc tcagatagca
    88381 agacaagcga ctgttttaaa ttctgatgaa aattttgcgg ttgtatttgc agcaatgggt
    88441 attacttttg aagaagctga gttttttatg gaagaactca gaaaaacagg agcgatcgat
    88501 cgttcggttt tatttatgaa cttggcaaat gatcctgcaa ttgagcgtat tgcaactccc
    88561 cgcattgctt taactgcggc agagtatcta gcttttgaaa aagatatgca cgttctagtt
    88621 atcatgacgg atatgactaa ctattgtgaa gcgttacgtg aagtctcggc agctcgccgt
    88681 gaagttccag ggagacgagg ctatccggga tatttatata caaatttatc aactctatac
    88741 gaaagggctg gtcgcttagt tggtaaaaaa ggttcggtga cacagattcc tattttaaca
    88801 atgccagaag atgacataac acatccaatt cctgatttaa ctggatacat tactgaaggg
    88861 caaattattt tgtcgcatga gttgtataat caaggttatc gtccaccaat caatgtttta
    88921 ccttctctct ctcgattaaa agataaggga tctggagaag gtaaaactcg tggagatcat
    88981 gctccaacta tgaatcaact gtttgcagcc tatgcccaag ggaaaaaggt tgaagagtta
    89041 gcagtagtat taggagaatc ggctttatct gatgtagata aattgtatgt gaggtttaca
    89101 aagcgttttg aagaagagta cataaaccaa ggattttata aaaatcgaaa tatagaagat
    89161 acgttgaatc ttgggtggga attactatca attcttccta gaacagagtt aaaacgtatc
    89221 aaagatgatt tgcttgataa atacttacct ttggtagaag tttaatccgg aaatggagtg
    89281 attatctatg gtacgtttga atgtaaaacc aactcgtatg gaattgaata acttaaagga
    89341 acgtttgaca acagctgaac gtggacataa gttattaaag gataaaagag atgaattgat
    89401 gaggcgattt atttctttga ttcgtgagaa taatcaactt cggaaagaag tggaaagtta
    89461 tctaattgat aatctaaaat cctttgcagt tgctaaatca ttaaagaatt ctcaaatggt
    89521 ggaggaatta ttttcaattc catcgaaaga aattgaatta tttgttgaga aagaaaatat
    89581 catgagtgta acagttccta gaatgcatat gaatattact tctcaaaatg agaacagtga
    89641 atacagctat ttatcttcta atagtgaaat ggatgatgta tttgctacaa tgaatagttt
    89701 aatttataaa ttactaagac tggcagaagt tgaaaaaacg tgtcagttaa tggctgatga
    89761 aatagaaaaa acacgtagac gtgtaaatgg tttagaatac tcgattattc caaacttgtc
    89821 ggaaactatt cattatatag aattgaaact agaggaggca gaaagagcca atttagttcg
    89881 tattatgaaa gtgaagtaga tcctttattt agattattaa ttagatgaac aaatatcagc
    89941 ttggataagg ctttaagcct ttctaagctt tttttattga cagtatcagg atatcttttt
    90001 caaaattttg gtttgttaga taatgaaaat gtttctacta atctagattt aggattagta
    90061 aatcgtaaat gtaattatat agaa
//
LOCUS       DAVDWM010000005        81536 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVDWM010000005 DAVDWM010000000
VERSION     DAVDWM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 81536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.005"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            51..299
                     /locus_tag="VUW49_000413"
                     /pseudo
     CDS             51..299
                     /locus_tag="VUW49_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            430..1602
                     /locus_tag="VUW49_000414"
     CDS             430..1602
                     /locus_tag="VUW49_000414"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET7980386.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1602..2876
                     /locus_tag="VUW49_000415"
     CDS             1602..2876
                     /locus_tag="VUW49_000415"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET7980387.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(2912..3877)
                     /locus_tag="VUW49_000416"
     CDS             complement(2912..3877)
                     /locus_tag="VUW49_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7980388.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIKNYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3858..5312)
                     /locus_tag="VUW49_000417"
     CDS             complement(3858..5312)
                     /locus_tag="VUW49_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET7980389.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5365..5571
                     /locus_tag="VUW49_000418"
     CDS             5365..5571
                     /locus_tag="VUW49_000418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980390.1"
                     /translation="MQAFYVKKEEKLSNDYHKINTGNQSNFYENVKDNEIKYFLTKVL
                     NLFFQGIPDETVKNNQSKACPLSG"
     gene            5689..7572
                     /locus_tag="VUW49_000419"
     CDS             5689..7572
                     /locus_tag="VUW49_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET7980391.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            7721..8608
                     /locus_tag="VUW49_000420"
     CDS             7721..8608
                     /locus_tag="VUW49_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7980392.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEISK"
     gene            complement(8928..9545)
                     /locus_tag="VUW49_000421"
     CDS             complement(8928..9545)
                     /locus_tag="VUW49_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7980393.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(9960..10173)
                     /locus_tag="VUW49_000422"
                     /pseudo
     CDS             complement(9960..10173)
                     /locus_tag="VUW49_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10658..10738)
                     /locus_tag="VUW49_000423"
                     /pseudo
     CDS             complement(10658..10738)
                     /locus_tag="VUW49_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10854..11747
                     /locus_tag="VUW49_000424"
     CDS             10854..11747
                     /locus_tag="VUW49_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980394.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11744..12943
                     /locus_tag="VUW49_000425"
     CDS             11744..12943
                     /locus_tag="VUW49_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7980395.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGSFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(13296..14063)
                     /locus_tag="VUW49_000426"
     CDS             complement(13296..14063)
                     /locus_tag="VUW49_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980396.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(14080..14775)
                     /locus_tag="VUW49_000427"
     CDS             complement(14080..14775)
                     /locus_tag="VUW49_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980397.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYILDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(14780..15145)
                     /locus_tag="VUW49_000428"
     CDS             complement(14780..15145)
                     /locus_tag="VUW49_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7980398.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            15285..15758
                     /gene="nrdR"
                     /locus_tag="VUW49_000429"
     CDS             15285..15758
                     /gene="nrdR"
                     /locus_tag="VUW49_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET7980399.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15759..16928
                     /locus_tag="VUW49_000430"
     CDS             15759..16928
                     /locus_tag="VUW49_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET7980400.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLVLHAPLSSQD
                     FLEHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSDPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            16929..17825
                     /gene="dnaI"
                     /locus_tag="VUW49_000431"
     CDS             16929..17825
                     /gene="dnaI"
                     /locus_tag="VUW49_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET7980401.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            17822..18535
                     /locus_tag="VUW49_000432"
     CDS             17822..18535
                     /locus_tag="VUW49_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET7980402.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQHHDMKPRLLLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            18549..19859
                     /gene="der"
                     /locus_tag="VUW49_000433"
     CDS             18549..19859
                     /gene="der"
                     /locus_tag="VUW49_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7980403.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            19950..20285
                     /locus_tag="VUW49_000434"
     CDS             19950..20285
                     /locus_tag="VUW49_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980404.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFCLRQE
                     LGKSKGGRH"
     gene            20425..20760
                     /locus_tag="VUW49_000435"
     CDS             20425..20760
                     /locus_tag="VUW49_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980405.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            20945..21736
                     /locus_tag="VUW49_000436"
     CDS             20945..21736
                     /locus_tag="VUW49_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980406.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            21729..22370
                     /locus_tag="VUW49_000437"
     CDS             21729..22370
                     /locus_tag="VUW49_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980407.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            22374..23006
                     /locus_tag="VUW49_000438"
     CDS             22374..23006
                     /locus_tag="VUW49_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7980408.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            23034..23321
                     /locus_tag="VUW49_000439"
     CDS             23034..23321
                     /locus_tag="VUW49_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980409.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            23467..25980
                     /gene="secA"
                     /locus_tag="VUW49_000440"
     CDS             23467..25980
                     /gene="secA"
                     /locus_tag="VUW49_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7980410.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            26061..27092
                     /locus_tag="VUW49_000441"
     CDS             26061..27092
                     /locus_tag="VUW49_000441"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7980411.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            27094..28125
                     /locus_tag="VUW49_000442"
     CDS             27094..28125
                     /locus_tag="VUW49_000442"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7980412.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            28164..28532
                     /locus_tag="VUW49_000443"
     CDS             28164..28532
                     /locus_tag="VUW49_000443"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7980413.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            28522..29625
                     /locus_tag="VUW49_000444"
     CDS             28522..29625
                     /locus_tag="VUW49_000444"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7980414.1"
                     /translation="MKASPHRPTKALIYLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            29644..31659
                     /gene="recG"
                     /locus_tag="VUW49_000445"
     CDS             29644..31659
                     /gene="recG"
                     /locus_tag="VUW49_000445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7980415.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            31687..31836
                     /locus_tag="VUW49_000446"
     CDS             31687..31836
                     /locus_tag="VUW49_000446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980416.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHRFEVADRTDEVS
                     NIYIR"
     gene            32174..33154
                     /locus_tag="VUW49_000447"
     CDS             32174..33154
                     /locus_tag="VUW49_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET7980417.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            33959..34186
                     /locus_tag="VUW49_000448"
     CDS             33959..34186
                     /locus_tag="VUW49_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980418.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            34474..37371
                     /gene="nanA"
                     /locus_tag="VUW49_000449"
     CDS             34474..37371
                     /gene="nanA"
                     /locus_tag="VUW49_000449"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET7980419.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLIFPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGNKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            38386..38838
                     /locus_tag="VUW49_000450"
     CDS             38386..38838
                     /locus_tag="VUW49_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7980420.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            38857..40194
                     /locus_tag="VUW49_000451"
     CDS             38857..40194
                     /locus_tag="VUW49_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7980421.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLGSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            40273..41157
                     /locus_tag="VUW49_000452"
     CDS             40273..41157
                     /locus_tag="VUW49_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7980422.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            41157..41990
                     /locus_tag="VUW49_000453"
     CDS             41157..41990
                     /locus_tag="VUW49_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7980423.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            41997..42068
                     /locus_tag="VUW49_000454"
                     /pseudo
     CDS             41997..42068
                     /locus_tag="VUW49_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            42184..43287
                     /locus_tag="VUW49_000455"
     CDS             42184..43287
                     /locus_tag="VUW49_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7980424.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRIRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            43461..44159
                     /locus_tag="VUW49_000456"
     CDS             43461..44159
                     /locus_tag="VUW49_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET7980425.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            44178..45695
                     /locus_tag="VUW49_000457"
     CDS             44178..45695
                     /locus_tag="VUW49_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET7980426.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            45820..47148
                     /locus_tag="VUW49_000458"
     CDS             45820..47148
                     /locus_tag="VUW49_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7980427.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            47400..48266
                     /locus_tag="VUW49_000459"
     CDS             47400..48266
                     /locus_tag="VUW49_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7980428.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            48282..49121
                     /locus_tag="VUW49_000460"
     CDS             48282..49121
                     /locus_tag="VUW49_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7980429.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            49393..49845
                     /locus_tag="VUW49_000461"
     CDS             49393..49845
                     /locus_tag="VUW49_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7980430.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYASMEEKVRKYLFKILID"
     gene            49987..50295
                     /locus_tag="VUW49_000462"
     CDS             49987..50295
                     /locus_tag="VUW49_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980431.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAERVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDAHDKIPNNGRINF
                     "
     gene            50582..51226
                     /locus_tag="VUW49_000463"
     CDS             50582..51226
                     /locus_tag="VUW49_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET7980432.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            51395..52312
                     /locus_tag="VUW49_000464"
     CDS             51395..52312
                     /locus_tag="VUW49_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7980433.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            52330..53214
                     /locus_tag="VUW49_000465"
     CDS             52330..53214
                     /locus_tag="VUW49_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7980434.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(53230..54081)
                     /locus_tag="VUW49_000466"
     CDS             complement(53230..54081)
                     /locus_tag="VUW49_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7980435.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            54281..56338
                     /gene="pbp2b"
                     /locus_tag="VUW49_000467"
     CDS             54281..56338
                     /gene="pbp2b"
                     /locus_tag="VUW49_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET7980436.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSELTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            56349..56945
                     /gene="recR"
                     /locus_tag="VUW49_000468"
     CDS             56349..56945
                     /gene="recR"
                     /locus_tag="VUW49_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET7980437.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            57121..58164
                     /locus_tag="VUW49_000469"
     CDS             57121..58164
                     /locus_tag="VUW49_000469"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7980438.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLVYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPKLIERLVDL
                     AKESFDKREAHLI"
     gene            58248..59621
                     /locus_tag="VUW49_000470"
     CDS             58248..59621
                     /locus_tag="VUW49_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7980439.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAKIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            59608..60219
                     /locus_tag="VUW49_000471"
     CDS             59608..60219
                     /locus_tag="VUW49_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7980440.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            60310..61014
                     /locus_tag="VUW49_000472"
     CDS             60310..61014
                     /locus_tag="VUW49_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET7980441.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            61233..62606
                     /gene="ftsA"
                     /locus_tag="VUW49_000473"
     CDS             61233..62606
                     /gene="ftsA"
                     /locus_tag="VUW49_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7980442.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKSGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            62623..63882
                     /gene="ftsZ"
                     /locus_tag="VUW49_000474"
     CDS             62623..63882
                     /gene="ftsZ"
                     /locus_tag="VUW49_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7980443.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            63887..64558
                     /locus_tag="VUW49_000475"
     CDS             63887..64558
                     /locus_tag="VUW49_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7980444.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            64568..65107
                     /locus_tag="VUW49_000476"
     CDS             64568..65107
                     /locus_tag="VUW49_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET7980445.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            65107..65370
                     /locus_tag="VUW49_000477"
     CDS             65107..65370
                     /locus_tag="VUW49_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7980446.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            65367..66152
                     /locus_tag="VUW49_000478"
     CDS             65367..66152
                     /locus_tag="VUW49_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7980447.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            66161..66901
                     /gene="divIVA"
                     /locus_tag="VUW49_000479"
     CDS             66161..66901
                     /gene="divIVA"
                     /locus_tag="VUW49_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET7980448.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELVEFEAQIKQEVEAPTPVVSPQVE"
     gene            67297..70089
                     /locus_tag="VUW49_000480"
     CDS             67297..70089
                     /locus_tag="VUW49_000480"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7980449.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNSPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLISLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWKRDTKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDAVAYEDLRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTSYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAETFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAAVSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            70498..70860
                     /locus_tag="VUW49_000481"
     CDS             70498..70860
                     /locus_tag="VUW49_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980450.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            71395..72087
                     /locus_tag="VUW49_000482"
     CDS             71395..72087
                     /locus_tag="VUW49_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET7980451.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            72140..74551
                     /locus_tag="VUW49_000483"
     CDS             72140..74551
                     /locus_tag="VUW49_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET7980452.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIKLSLTCDLASDEKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGYFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            74801..75502
                     /locus_tag="VUW49_000484"
     CDS             74801..75502
                     /locus_tag="VUW49_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980453.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            75504..78212
                     /locus_tag="VUW49_000485"
     CDS             75504..78212
                     /locus_tag="VUW49_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7980454.1"
                     /translation="MIKRKTYWKDLIQSFTGSKERFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGIVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQILL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKDGLQNITLMMIEKEDLTSFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVATVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(78308..78799)
                     /gene="tpx"
                     /locus_tag="VUW49_000486"
     CDS             complement(78308..78799)
                     /gene="tpx"
                     /locus_tag="VUW49_000486"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET7980455.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(78922..79851)
                     /gene="psaA"
                     /locus_tag="VUW49_000487"
     CDS             complement(78922..79851)
                     /gene="psaA"
                     /locus_tag="VUW49_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET7980456.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(79883..80731)
                     /locus_tag="VUW49_000488"
     CDS             complement(79883..80731)
                     /locus_tag="VUW49_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7980457.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(80728..81450)
                     /locus_tag="VUW49_000489"
     CDS             complement(80728..81450)
                     /locus_tag="VUW49_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET7980458.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        24545 a        14942 c        18063 g        23986 t
ORIGIN      
        1 tactttcctt attgtctgta agtctactga ccttgttgtt tatcccagtc atggtttcta
       61 gttggggctc agagtttcaa agtggatggt aagagcatca attgattgct gagaaggtta
      121 gtaaaacact tgacaagaca tttgataagg atgtcagaga aattccgacc agtcagtttt
      181 atcaaaaatt tgtagatgag atgggaagga cttactcagg aaatttgatc ctccaggagc
      241 tgataactgt gaatggagct tataaagcta cttatatcgg tgaactctct agcaactaat
      301 cattctttaa aatgatgacc aataagaggc ttgatttggt gaatcaagtc ttttttctct
      361 cttttactgc catttgtgat aaaatagtat agaacgattt tttacatgaa tgataaaaca
      421 gaggtaaata tgacaatcgg tattgataag attggttttg cgaccagtca atatgtcttg
      481 aaattacaag acttagcaga agcgagggga attgaccctg aaaaattaag taaaggactc
      541 ttactcaagg aattgagtat tgcgccccta actgaggaca ttgtgacctt ggcggccagt
      601 gctagtgact ctattttaac tgagcaagaa agacaagaag ttgacatggt cattgtggct
      661 accgagtcag gaattgacca gagtaaggct gcggccgtct ttgtgcatgg cttgctgggc
      721 atccagccct ttgctcgtag tttcgagatt aaagaagcct gttatggagc gactgctgcc
      781 cttcattatg ccaaattgca tgtggaaaat tctccggagt ccaaggtctt ggtcattgcc
      841 agtgatattg ccaaatacgg tattgaaact ccaggagaac caactcaagg tgctggaagt
      901 gtagctatgt tgattacaca aaatccacgc atgatggcct ttaataatga caatgtagct
      961 cagacccgtg acatcatgga tttctggcga ccaaattact cgacaactcc ttatgtaaat
     1021 ggtgtttatt ctacccaaca atacttggat agtttgaaaa cgacttggct tgaatatcaa
     1081 aaacgctacc agcttactct ggatgatttt gcggctgttt gtttccactt gccttatcct
     1141 aaattagcgc taaaaggctt gaaaaaaatc atggataaga gcctgcctca agagaaaaaa
     1201 gacctcttac aaaagcattt tgaccagtct attctctaca gtcaaaaggt ggggaatatc
     1261 tacacaggtt cacttttcct tggacttttg tctctcttgg aaaatacaga tagcttgaaa
     1321 gctggggata aaatcgccct ttatagttac ggaagtggag ctgtggctga gttcttcagt
     1381 ggtgaattgg ttgaaggata tgaagcttat ttggataaag accgcttgaa caagctcaac
     1441 caacgaactg ccttatccgt tgcagactat gaaaaggtct tttttgagga agtaaacttg
     1501 gatgaaacaa actctgccca gtttgctggc tatgaaaatc aagattttgc cttggttgaa
     1561 attctcgacc accaacgccg ttatagcaag gttgaaaaat aatgaagata agttggaatg
     1621 gattttctaa aaaatcatac caagagcgcc tcgagctgtt aaaagctcag gcgctcctta
     1681 gtcctgagag acaagctagt ctggagaagg atgaacagat gagcgtgact gtggcagacc
     1741 agctgagtga gaatgtagtg ggaacttttt ctctgcctta ttcgctggtt ccggaggtac
     1801 ttgtcaacgg tcaggaatac accgttccct atgtgacaga agaaccctct gtggttgcgg
     1861 cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg ttttactgca caagtccatc
     1921 agcgacagat gattgggcag gtagcccttt atcaagttgc taatcctaaa ctagcgcaag
     1981 agaagattgc cagcaagaaa gcggagctct tggagcttgc caatcaagcc tatccttcta
     2041 tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga gcagataaaa ggcgaaccag
     2101 actttctcgt tgtttatatt catgtcgata cccaggaagc catgggtgcc aatatgctca
     2161 acaccatgct ggaagccttg aaaccagtct tagaagaact cagtcaggga cagagtctca
     2221 tgggaatcct gtccaactac gcgaccgatt ctctggtgac tgcaagctgt cgcatcgcct
     2281 ttcgctactt gagccgccaa aaggatcaag gacgagagat tgcggagaaa attgcgttgg
     2341 ctagtcagtt tgcgcaggct gatccttacc gagctgctac tcataataaa ggaattttta
     2401 atggtattga tgcgattttg attgccactg gtaatgactg gcgtgccatc gaagctgggg
     2461 cccatgcctt tgccagtcga gatggacgtt atcaaggtct tagttgctgg acgctggacc
     2521 ttgaaagaga agaattggtc ggtgagatga ccctgcccat gcctgtagcg actaagggtg
     2581 gctctatcgg cctcaaccca cgtgtagctc tcagtcatga tctactagga aatccttctg
     2641 ccagagaatt agcccagatt atcgtgtcca tcggtcttgc ccaaaatttt gcagctctca
     2701 aagccttggt aagtacgggc atccagcaag gccacatgaa actacaggcc aaatccctag
     2761 ctctcctagc tggggctagt gaatctgaag ttgctcccct agtcgagcag cttatcgcag
     2821 ataaaacctt taacctagag acagcccagc gctatctcga aaatttaaga tcataaaaaa
     2881 ctcagacgaa tcggtctgag ttttcttgtg tttaaatact ttttcctggt aatagtgtaa
     2941 ctggtaagaa gtaaccagtt gtggcgactt ccttgccttc aatcttttgc aagagaagat
     3001 caatagtgag acaagcaatc tcttccaaag gttgcttgat agtagccaat tgagggtagt
     3061 aatttttgat aaagtaggtc ccatcatagc cgatgacctt gagctctttt gggacagaaa
     3121 tgcccaattc ttgagcgatt ttaatgacca gaatagctgt caaatcatcc gaagcaaaaa
     3181 tggcatctgg tttttcccgg gtcaagatat tcttgatttc catttctttt ctgacgggag
     3241 aaaagtcact ggaaacattg ataataggag cttttgggag tacggatgca aaaccagcgt
     3301 ggcgcagtcc ggttggcgaa ttggaattgt cattccctgt aatcatgatg atagactggg
     3361 cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc agcatagttg tcagaggaga
     3421 caacagggat gtctggcgat aggtttcggt caaaggaaat aatcggcgct gtcacacgat
     3481 tgtagtcttc gattcctagg ttgtgactac cagaaatgat gccgtctacc tgattggctt
     3541 ccaacatttc gatgtattcg cgttccttct cagaatcatg ttcactgttg cagatgatgg
     3601 tcttgtaacc atttttgaag agttggtgtt ccaatttatc aatcaattct gcatagaaaa
     3661 cattggaaat attggggaaa atcaagccga ttaacttagc tgattttcct tgcagactac
     3721 gagccaggtt gttgggttta tagcctaatt ctctcatggc ttcattgact ttttggatgg
     3781 ttttctcaga tagatatcct tttttattga taacccgaga aacggtagta ggactgacgc
     3841 ctgcaagttt ggcgacatca gttagttttg cgaccataat ctaattcata gtaagttcca
     3901 gttgggtttc cagatttgat caggatacca ttttggtccg catgtgggaa gacacgacca
     3961 gaaaatactt tttctccttt attgatgaaa atttcaaaga cagagttatc gatgaagatt
     4021 gtagcagtag tagcctgatt ctcgataggg caagaacgag ttgtcccaaa ttcttgggca
     4081 tactgttctc cagcctggct acgatccact gtcacttgac cgtttacaag gtcaaagttg
     4141 attgaaagtc ccttaccttc tttatcagca agtaagacaa tctcgctctg gctattagct
     4201 tccaagttga gttcaagttc gtaagtgttc ttggtttggg aacggtttga gaaggcttct
     4261 tcagaagcac gaaggtcctt aatagcagcg actggatact ggtagagctt gtcgtctttg
     4321 atagtgagtt ccttgaccaa agagaaggtt ccttggtggt caaaacggtc agatgggtaa
     4381 gaaacatctg gcaaaccaag ccagctaact gctagagcac gcccatcagg agcgttgaag
     4441 gcttgagttg cataggcttc gaaaccgtaa tccatgtttt gaagttgaga cacatctacc
     4501 attttggcat ttttagggtc aaaggaagcc ccaatcttat acatatttgg aaagatatta
     4561 tcgtagtcta gaactttctt atccaatcct tgtggacagt agagaaggac aggttgttcc
     4621 tctacaaaga ccagattagg acattccatc atgtaggcag tacggtcgtt agcaaagtca
     4681 aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga cagccttgta gagacgaacg
     4741 aaaccttttt tctccaagtc ctgtccaccg acgacagcat agtattgacc tttaaagtta
     4801 aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg gctggtcaat caagatcttg
     4861 tcaatctttg taatcttacc ctccttgtcc atcaaagcac cgatctggta tgggtgacgg
     4921 atccagtttt catcgcgaac atttcctgta taaaatagga ataagttatc gccaaattgc
     4981 atggcagaac cagagtaggc accgtggcta tctaatggag tatctggtaa aactttgatt
     5041 ccagtttctt taaagtgaac caaatcatca ctttctagct gtgcccaaga tttcaaaccg
     5101 tgggctgcac caaaaggaaa attctggtaa aagaggatcc acttgccatc aaagtaagaa
     5161 aagccatttg ggtcgttgag aagtcctgtt tttggctcaa catggtaatg agtatgccat
     5221 ggagattgtg ccatattttc ctttatatgt tgaatttctt cttgcgtcca atcttgataa
     5281 agtctgtaac gacgctcagt tgtccattcc attcattcct ccaaaaatct tatcactttt
     5341 aatagtaact gttttcgata aaaaatgcaa gccttttatg ttaaaaaaga ggaaaaactg
     5401 tcaaacgatt atcataaaat aaatacagga aatcaatcaa atttttacga aaatgtgaaa
     5461 gataatgaaa taaaatactt tttaaccaag gttttaaact tattttttca aggaattcct
     5521 gatgaaacag tcaaaaacaa tcaatctaaa gcttgcccac taagtggata gaatattttt
     5581 ctgcaaaacg cttgacatat ttttaaaaca tgataaaata atagtcgtaa gggcgaataa
     5641 aatcgctttc aaacaagaac aaaatgttat ataaggagat ttttgcaaat gaacaatcag
     5701 gaaattgcaa aaaaagtcat cgatgccttg ggcggacgtg aaaatgtcaa tagtgttgcc
     5761 cactgtgcga ctcgtctacg tgtcatggtc aaagatgaag agaaaatcaa taaagaagtg
     5821 attgagaact tggaaaaagt tcaaggtgct ttctttaact cagggcaata ccaaattatc
     5881 tttggtacag gtacagttaa caaaatgtac gatgaagttg ttgtacttgg attaccaaca
     5941 tcatctaagg atgacatgaa agcagaagtt gctaaacaag ggaactggtt ccaacgtgct
     6001 atccgtactt ttggtgatgt tttcgttcca atcatcccag ttatcgtagc gacaggtctc
     6061 ttcatgggtg tgcgtggtct tttcaacgct cttgaaatgc cacttccagg tgactttgca
     6121 acttacacac aaatcttgac agatacagcc ttcatcatct tgccaggttt ggttgtgtgg
     6181 tcaaccttcc gtgtatttgg tggaaatcct gccgttggta tcgttcttgg tatgatgctt
     6241 gtctctggct cacttccaaa cgcttgggca gttgctcaag gtggtgaagt aacagcaatg
     6301 aatttctttg gtttcatccc tgttgttggt ttgcaaggtt ccgttcttcc agccttcatc
     6361 atcggggttg tcggagctaa atttgaaaaa gctgtccgca aggttgttcc agatgtcatt
     6421 gacctcttgg taacaccatt cgtgacactt ttggtcatgt ctatccttgg actctttgtc
     6481 attggaccag ttttccacgt tgttgaaaac tacatcctta ttgctacaaa agcgattctt
     6541 agcatgccat ttggtcttgg tggtttcttg attggtgggg ttcaccaatt gatcgtcgtg
     6601 tcaggtgtgc accacatctt caacttgctt gaagtgcaat tacttgctgc tgaccatgct
     6661 aacccattca acgctatcat cacagctgct atgacagctc aaggtgctgc tactgttgcg
     6721 gttggtgtta aaacaaaaaa tccaaaactg aaaacacttg ctttcccggc tgctctttct
     6781 gccttcctag gtattacaga gcctgctatc ttcggggtga acttgcgctt ccgtaaacca
     6841 ttcttccttt cattgattgc tggtgcaatc ggtggtggat tggcttctat ccttggactt
     6901 gctggtactg gtaatggtat caccatcatc cctggtacaa tgctttatgt tggtaacgga
     6961 caacttccac aataccttct tatggtagct gtatcatttg cccttggttt tgctcttact
     7021 tacatgtttg gttacgaaga tgaagtagac gcaactgcag ctgcaaaaca agctgaagtg
     7081 gctgaagaaa aagaagaagt tgcgccagca gctcttcaaa atgaaacact tgtaactcct
     7141 atcgtcggtg atgttgtcgc tcttgctgat gtcaatgacc cagtcttctc aagtggagct
     7201 atgggacaag gtatcgctgt gaaaccaagc caaggcgtgg tctatgcacc agctgatgct
     7261 gaagtttcaa ttgcctttcc aacagggcac gcttttggtt tgaaaacaag aaatggtgct
     7321 gaagttttga ttcatgttgg tattgatact gtatctatga acggtgacgg ttttgaaaca
     7381 aaagttgctc aaggtaataa ggtgaaagct ggcgatgttc ttggaacatt tgactcaaac
     7441 aaaatcgctg cagctggact tgatgataca acaatggtta tcgttacaaa tacaggtgac
     7501 tacgcttcag tagctccagt cgcaacaggt tcagttgcta agggggatgc tgtgatcgaa
     7561 gtgaaaatct aatcaatcct ctctaatgtg aaaacgaaca aatgacatat ttgttcgttt
     7621 ttattagata gtaagattta cagaggaaaa tctaaaaaat agagacattt agactttcga
     7681 agtatgctat aataaagaaa ataaaaacaa gaggtttatc atgacaaaat tatatggaag
     7741 cttggaagcg ggcggtacaa agtttgtctg tgctgtcggt gatgaaaact ttaacgttgt
     7801 agaaaaaaca caatttccaa caacaactcc aatcgaaaca atcgataaaa ccattgagtt
     7861 cttctcaaaa ttcgataacc ttgctggtct tgcagttggt tcatttgggc cgattgatat
     7921 tgacaaaaac tcaaaaactt atggctttat cacgacgact ccaaaaccaa actgggcaaa
     7981 tgtggacttg cttggtgccc ttcgtcgcgc cctaaatgtg ccaatgtact tcacaacaga
     8041 cgtaaacagc tctgcttacg gtgaagtggt tgcccgtaac aatgctggtg gtcgtatcga
     8101 aaacttggtt tactacacaa tcggtacagg tatcggtgca ggtgtcatcc aacgtggtga
     8161 gtttatcggt ggtgtgggtc accctgaaat gggtcattat tatgttgcta gacacccaat
     8221 ggatattgaa aaagagttta agggtgtttg tcctttccat aagggatgtc tagaaggtta
     8281 tgcagctggt ccaagtttgg aagctcgtac aggtgtacgt ggggaaacta ttgaactcaa
     8341 caaccctgtt tgggatgttc aagcctacta tatcgctcaa gctgcggtta atgcgacagt
     8401 gactttccgc ccagacgtga ttgtctttgg tggaggggtc atggctcaac aacatatgct
     8461 ggaccgtgtc cgtgagaaat ttacatctct tcttaatggt tacctaccag taccagatgt
     8521 gcgtgactat atcgtgacgc cagcagtcgc aggaaatggt tctgccacac ttgggaactt
     8581 tgttcttgca aaagaaattt caaaataaag cacaaaatcc gcttgggaaa ccaaacggat
     8641 tttgtggttg aaaacaatat ttttgagcct ttccttaagt gattacggac ggtagcgact
     8701 ttcttcgaaa ttccatacct aaactttgag acactaggct caaagtttcc gaatacctga
     8761 aaccattaga tttcaggtgt ttctataacg gcggaaaggc taggtaagtc ctcgcctcac
     8821 tatcgaaaaa tatgcatgat ttccatacca aagtatattt agtatcaagc tatagttaat
     8881 ctacgcttta aaaatccact tgggaaacca aacggatttt ttacgtgtca aagcatttgc
     8941 cagaaaaagt caataatata ggtcaaaccg taggtataaa ccacgagggt aaagggtttg
     9001 gtcagaatga tgatggtcat gtagcgcttg aaactcatct tggtcagggc agccagcata
     9061 cagagaaagt cagctgggct aatgggccaa atcatcataa aaataaagaa gcggtcaaaa
     9121 cgattgccct tatctagcca gccgatgtac ttgtcgtagg tgcgcttgct gacgacagac
     9181 tggacaaagg cagctccgta taggcgcact agataaaaga taatggcaca gccaatcacg
     9241 atgccgatat agttgtagat agtcccgatg atgtgcccgt agataaagac cccagccacc
     9301 gaggtcaagg cccctggaat gatagggacg acagtctgta aaatctgtaa aaagataaag
     9361 agaggtggac cccagatgcc tgcctgctgg ataaaggcag agagggtttc cttggattgt
     9421 aaaatcccag cctgataagc ccaaatacag aaaatcaaac tcccaacccc accgacaatc
     9481 gatgagatat tgataactcg ctgcagaagg ggagaaacag ctttcatttg tttatcctgt
     9541 gacatgtaaa ctcctcatag atagtatatt tctactatac catattttgg aggagaaagg
     9601 gggaatgagt cttttttgat aaggaataga aagtaaatag cccttcgaga cttagaatag
     9661 gtctgtcaac taaaggttat atgcattcta ggtttcgaat aatcgcaatt ccataaattt
     9721 aaaagcattc taagtttcga atagcttatc tgagataatt ttgaggcatt cgcgctttcg
     9781 aatagtcatt ttccctaaag tttagatgta ttctgaagct agaaagacta aaatctaaaa
     9841 gtagtaagag tctctctctg atggacgtaa tttatggcta gtatgaatcc tttgaattgt
     9901 tagttaattt taatattaat cagttctgaa ataattacta aatatagtta gatggtgaat
     9961 taattattgc taaatttcaa gttcaagtta gtcatccaga agtcttctct gggggacttg
    10021 tagttcaagc attttttagg atagttgtta atccactttt cgatgaatgc gacttccttt
    10081 gtagtcgttt tcttagttcc ctgaggtaac catctatgaa tgaacttgtt gtgattttta
    10141 ttagatccta tagtggtgtg cataatagat ataatagatt gaaactagaa tagtacgcat
    10201 ctacttctaa aacattgtta gaaatcgatt tgactgtccc gatctatttg tcctgttttt
    10261 atttcatttt actatataat ccttagaaaa catatcggac aagcggttga attccgttcc
    10321 gtccattagt agataaagga ttactccttc ttgaaatgac agatgaactt gtttcttata
    10381 aaaatgttgt ctgaaactat tcgctttttt ccagaagttt tagaggaaga aaactttatg
    10441 aggaaaaagg agttactcaa ataacaattc aggattaaaa atagacagtt gaggagcgga
    10501 aggtataaat taagtttgct actgtataat ggatttatca ctaatactct tcgaaaatct
    10561 cttcaaacca cgtcagcttc gccttgccgt agatatagtt actgacttcg tcagttctat
    10621 ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tttatctgca acctcaaagc
    10681 tgtactttga gcagcctacg gctagcttcc tagtttgctc tttgcttttc attgagtata
    10741 aaagtgctcc aggccatatt ttacacagca atagtcttct aaaaatggac aaaaacattg
    10801 taaaggctat caaaaaggag tataatgagt gcaagaaatt tcggaggtga aagatgctag
    10861 aagtaagaag tctagagaaa agttttggct ccaagcaagt tttgtttggt attgactttc
    10921 aagcgcgacc aggtcgtatt ttgggactag tcggaaaaaa tggtgctgga aaaacaacga
    10981 ttttccatag tattttgaaa ttcctagaat atcagggaga aatcggtctg gatggtcagg
    11041 atattcgtca ggagacctat gctcggattg gttatctgcc tgaagaacgc agtctcatgc
    11101 ctaaattgac agttcttgaa caagttcgct acttggcgac tctaaaaggc atggatgcca
    11161 aagaagtcaa agaaaaactc cctcaatgga tgaagaggtt ggaagtgaaa ggaaagctga
    11221 cagataaaat caagagtctg tcaaaaggaa atcagcagaa gattcagctc attattactc
    11281 tgattcatga accagacctg attatcttgg atgagccttt tagtggattg gacccagtta
    11341 atacagaatt gctcaaacaa gtcatttttc aggaaaaaga gcgcggagca accattatct
    11401 tttctgacca tgtcatgacc aatgttgagg aactttgtga cgatattctg atgatccgag
    11461 atggccgtgt ggtcttgcac ggaccagttc aggatgtccg caatcaatac gggaaaacgc
    11521 gtctctttgt ttcaagtgaa cgaagcaagg aagaattgga aaatcttcct catgtcaaac
    11581 aggtgagctt gaccaaacaa ggcagttgga aattgatttt ggaggatgag agcgctggaa
    11641 gggaactctt cccaatcttg actcaaggtc aatatatcgc aacatttgac caacaagcgc
    11701 caactatcga tgaaatcttt aaactagaat caggagtgga agtatgagaa atatgtgggt
    11761 tgtaatcaag gaaacctatc ttcgacatgt caagtcatgg agtttcttct ttatggtgat
    11821 ttcgccgttc ctctttttag gaatctctgt aggaattggg catatccaag gttcttctat
    11881 ggctaaaaat aataaagtgg cagtagtgac aacagtgcca tctgtagcag aaggactgaa
    11941 gaatgtaaat ggtgttaact tcgactataa agacgaagca agtgccaaag aagcaattaa
    12001 agaagaaaaa ttaaaaggtt atttgaccat tgatcaagaa gatagtgttc taaaggcagt
    12061 ttatcatggc gaaacatcgc ttgaaaatgg aattaaattt gaggttacag gtacactcaa
    12121 tgaactgcaa aatcagctta atcgttcaac tgcttccttg tctcaagagc aggaaaaacg
    12181 cttagcgcag acaattcaat tcacagaaaa gattgatgaa gccaaggaaa ataaaaagtt
    12241 tattcaaaca attgcagcag gtgccttagg attctttctt tatatgattc tgattaccta
    12301 tgcgggtgta acagctcagg aagttgccag tgaaaaaggc accaaaatta tggaagtcgt
    12361 tttttctagc ataagggcaa gtcactattt ctatgcgcgg atgatggctc tgtttctagt
    12421 gattttaacg catattggga tctatgttgt aggtggtctg gctgccgttt tgctctttaa
    12481 agatttgcca ttcttggctc agtctggtat tttggatcac ttgggagatg ctatctcact
    12541 gaataccttg ctctttattt tgatcagtct tttcatgtat gtagtcttgg cagccttcct
    12601 aggatctatg gtttctcgtc ctgaggactc agggaaagcc ttgtcgcctt tgatgatttt
    12661 gattatgggt agtttttttg gagtgacagc tctaggtgca gctggtgaca atctcctctt
    12721 gaagattggt tcttatattc cctttatttc gaccttcttt atgccgtttc gaacgattaa
    12781 tggctatgcg gggggagcag aagcatggat ttcacttgct attacagtga tttttgcggt
    12841 ggtagcaaca ggatttatcg gacgcatgta tgctagtctc gttcttcaaa cggatgattt
    12901 agggatttgg aaaaccttta aacgtgcctt atcttataaa tagaagagcc ttgcgaaagc
    12961 gaggttttta aaggtgaaaa gggtggaatc acgaacctaa ttatgtacac attatgtaaa
    13021 caggttgctt gaaaactcaa ttgtatagtg aactgaatct ggaataggat aatattactt
    13081 ctaaaacatt cttataaatt gatttgaatt ccaaaatccc atcgttccgt tcttatttca
    13141 ttttgcgata tagtgaaagg aaataagtca gctcacaaaa aacgcataat atcaaggatt
    13201 tttaacactt gatactatgc gttttctaat tttaaagact ttttgcctgg tctcttttgt
    13261 tctatgagta tgttacatat ctaggtaaaa attatttaaa gcaaattaac cttatttctc
    13321 aagatgtagt aggttccgag ataaaagata gctatgaata tgagttcttt aacaatggct
    13381 atacctgctc ccatatagaa atggtcattg agtcctacca gtgaattgac atagaaatta
    13441 gaactaagat tgaagaaaag ttcaataaat ccaatgacga tttggatacc aatgtaggct
    13501 gcaacagcga gtgctgtacg gtattcattg aaaagctgtc caatggaaat agccaggtag
    13561 atgcagagga ttcctgaaat ggtatttagt aggaaggata tacctgtaag aaagatctga
    13621 gggagatgtg tttctacaaa tgtaatcaca taagaaagag gaatccattc tggagctgtt
    13681 aaagccagaa taataacagc acttagagcc aatacagcgg tgctaatcaa tgaccagata
    13741 aaggcaccga ttagtttggc tgtgatgata tggtgttcag aaactggcaa ggtcaaagtc
    13801 agatagcctt gtcggtcgta gacactacct ttgaagcgtt taataatcaa gaaaatggtt
    13861 gaaatcgcaa gtgtaagcat caagccacca aagactgtag ctagaaaaag tagcatagta
    13921 gcctgacttt cttggtaagg tagattttta aagccttgtg tctgtattcc gatgagggca
    13981 gaaaggacta gcacggctgc gtagagggct aaataccact tgttaacatt tttaaattcg
    14041 tagtgaacta aattccaaaa cataaataat ctcctttgct taggccttaa attcctgacg
    14101 gaagagttgg tcaatggatt cacctgactc gtagcgaata tcatctacat ttccttgacg
    14161 gacgactttt ccgtctttta ggaagacaat ttcatccaag attggctcga tatcagaaat
    14221 caagtgggta gaaatcaaaa cggtagaagt tggtgagtag ttgttgataa tggtattgag
    14281 gatataagca cgggctgctg gatccacccc accaatgggt tcatccaaaa tatagagacg
    14341 agcatcacgg ctcataacca aaatcagttg aaccttttct ttgtttcctt ttgatagttt
    14401 cttgagacga ctattttcat caatgcccag gtctgcaagt agatgatggg cgcgttcaag
    14461 attgaaatct ttatagaagg tcttgaagta ggttagggct tctttgacct tcatttgctc
    14521 attgagatag gtcgtatcag gcaaataagc tacaacggcc ttggttgctg ggcttgggtc
    14581 catgtcgttg atgaggacac gtccttgatc tggttgtaag aggccattaa ttagtttaat
    14641 cagggttgtt tttcctgagc cgtttggccc aagaaggccg acaatttttc cagctggaat
    14701 gtcaagagaa acattttcaa gggctggtgt tgctccatat gatttggata cattttcaaa
    14761 tgctagtaat gacataggct taaactcctt taatataatc actgactacg cctggtagtt
    14821 cttctttttc atagccaaaa tgggtcatgg aggaaacgaa gtgttccaat tcttcttctg
    14881 ataattgttt gcgtgactga gcgattagct ccttatcctt agtcacaaat cgtccagttg
    14941 ttcgtttgct gtagacaaat ccttctcgtt caaggtctga taaggctctt tggatggtat
    15001 tgggattgac accagcctcg ctagctagct ccctcacggt tggaagttgt tgattgggtt
    15061 ccagtgtatg ggaaacaatc tgaagcttga ttttctccat aatctgtaaa tagatgggtt
    15121 ttttgttgtc aaatgtccag gacatcttag tctcctttct cttacccttt tgtcttaatt
    15181 gaataataca acaaaacaaa aaacttgtca agcaaaaaga agaattgttt tgggaatcgc
    15241 ttaaaaaatg gtataatgaa aagaaaacga taaggaggga aaggatgcgt tgtccaaaat
    15301 gtggggctac caagtcaagt gttatcgata gtcgccaagc agaagaaggg aacaccattc
    15361 gtagaagacg tgagtgcgac gaatgccaac accgttttac aacctacgaa cgagtagaag
    15421 aaagaacctt agtggttgtt aaaaaagatg gcacacggga acaattctcc agagataaaa
    15481 tctttaatgg gattatccgc tcagcccaga aacgtcctgt gtcaagtgat gaaatcaaca
    15541 tggtagtcaa tcgtatcgaa cagaaactcc gtggtcgaaa tgaaaatgaa attcaaagtg
    15601 aggacattgg ttcactcgtc atggaggagt tggctgaatt ggacgagatt acctatgtac
    15661 gttttgctag tgtctatcgt agttttaagg atgtcagtga gttagagagc ttgctccaac
    15721 aaatcaccca gtcctctaaa aagaaaaagg aaagataaat gaagccaatt gaccgttttt
    15781 cttatctaaa gaataatcgg gtgtcgcaag atacctcatc tctggtacag tgctacctcc
    15841 cgattatcgg tcaggaggca ctgagccttt atctttatac aatcagtttt tgggataatg
    15901 gtagaaagga atatcttttt tcaagcattc tcaaccatct taactttggg atggatagac
    15961 tgataaaatc attgaaaatc ttatctgctt ttaatctctt gaccctctat caaaaggggg
    16021 atgtttatca gctagtcctc catgctcctc tatctagtca agacttcttg gagcatcctg
    16081 tttatcgcag actcttagag aaaaagattg gcgatgtagc tgtggaggat ttgaaagttg
    16141 aaagtgctga tggtgaagaa atacctgtct cactcaatca agtctttcca gaattggcag
    16201 aactaggcag tcaagaagac cttggtctca agaagaaagt ggccaacgat tttgacttgg
    16261 accattttcg ccagctgatg gctcgagatg gacttcgttt tgcggatgag cagtccgatg
    16321 tcttaaacct ctttgctatt gccgaggaga agaaatggac ttggtttgaa acctatcaat
    16381 tggccaagtc aacagctgtt tctcaggtta tttcaaccaa acgcatgcgg gaaaaaattg
    16441 ctcaaaaacc agtttcctct gactttagtc ttaaggaaac aaccattatc aaagaagcca
    16501 aaagtaaaac tgccctgcag ttcttggcag aaatcaagca aacacgcaag ggaaccatta
    16561 cccaaacaga aagagaactc ttgcaacaga tggctggctt gggcttgctg gacgaagtca
    16621 tcaatatcat tcttttattg acctttaata aggtcgattc ggcaaatatc aatgagaaat
    16681 atgccatgaa ggtagccaat gactatgcct atcaaaagat tcattcggca gaaaaggcag
    16741 tcttgcgcat ccgtgagcga ggacagaaag caaaaacaca aaaacagaat cagactgccc
    16801 cagtaaaaac caatgttcct aaatggagtg atcctgaata taagaatgaa accagcgagg
    16861 aaactcgtct ggaactagaa cgtaagaagc aagaactatt agctcgatta gaaaaaggag
    16921 gagattagat ggaaagtgtc ggagacgtac tcaaacgtca acccagccgt ttttattatc
    16981 aagatttggt ccagaaaatc atgaaggacc ctgatgttgc ggcttttatc cagcaagaat
    17041 ccttgactcc agaggaatta aatcgcagta tctccaagtt taatcagtac atcaccgagc
    17101 gtgacaagtt tctccgtggg gatacggatt atattgccaa aggctacaag ccgattttgg
    17161 ttaagaatca tggctatgca gatgtttcat atgaagaaac tcctgagcta atcgcggctg
    17221 aaaaagaagc ggctattaag aaccgtctca agttaatcaa tctaccagcc agtctcaaga
    17281 aagctagttt ggctcaagtt gacttggatg atttggggcg cttgccagtt tttgaaaaac
    17341 tattagcctt tgtggagcaa tatccagcta ttcgaaaagg tctttactta tatggagact
    17401 ttggtgtggg taaaagtttc atggtggctg ccctagctca tgatttatca gaaaaacgtg
    17461 gtgtttcatc aactctcctc cactatccta gctttgtcat tgatgtcaaa aatgctatca
    17521 gtgatggcaa tgttaagacc ttggtggatg agattaagct gtctgaggtc ctgattttag
    17581 atgatattgg cgccgagcaa tcaaccgttt gggtgcgtga cgaaatcctg caggtcattc
    17641 tccaatatcg gatgcaggaa aatttaccga cctttttcac atccaacttc aactttgaag
    17701 atttggagaa gcattttgcg aaagtgaaac atggaaatga cgaaacctgg gaagccagac
    17761 gggtcatgga acgcatccgt tatttggctg aggagactcg tttagaagga gtaaaccgtc
    17821 gatgacagaa accattaaat tgatgaaggc tcatacttca gtgcgcaggt ttaaagagca
    17881 agaaattccc caagtagact taaatgagat tttgacagca gcccagatgg catcatcttg
    17941 gaagaatttc caatcctact ctgtgattgt ggtacgaagt caagagaaga aagatgcctt
    18001 gtatgaattg gtacctcaag aagccattcg ccagtctgct gttttccttc tctttgtcgg
    18061 agatttgaac cgagcagaaa agggagcccg acttcatacc gacaccttcc aaccccaagg
    18121 tgtggaaggt ctcttgatta gttcggtcga tgcagctctt gctggacaaa acgccttgtt
    18181 ggcagctgaa agcttgggct atggtggtgt gattatcggt ttggttcgat acaagtctga
    18241 agaagtggca gagctcttta acctacctga ctacacctat cctgtctttg ggatggcact
    18301 gggtgtgcca aatcaacatc atgatatgaa accgagactg ctactagaga atgttgtctt
    18361 tgaggaagaa taccaagaac agtcaactga ggcaatccaa gcttatgacc gtgttcaggc
    18421 tgactatgct ggggcgcgtg cgaccacaag ctggagtcag cgcctagcag aacagtttgg
    18481 tcaagctgaa ccaagctcaa ctagaaaaaa tcttgaacag aagaaattat tgtagaaagt
    18541 gagaaattat ggccctacca actattgcca ttgtaggacg tcccaatgtt gggaaatcaa
    18601 ccctatttaa tcggatcgct ggtgagcgaa tctccattgt agaagatgtc gaaggagtga
    18661 cacgtgaccg tatttatgca acgggtgagt ggctcaatcg ttcttttagc atgattgata
    18721 caggaggaat tgatgatgtc gatgctcctt tcatggaaca aatcaagcac caggcagaaa
    18781 ttgccatgga agaagcagat gttatcgtct ttgtcgtgtc cggtaaggaa ggaattaccg
    18841 atgcagacga atacgtagcc cgtaagcttt ataagaccca caaaccagtt atcctcgcag
    18901 ttaacaaggt agacaaccct gagatgcgaa atgatatcta tgatttctat gccctcggtt
    18961 tgggtgaacc actgcctatc tcatctgtcc atggtatcgg tacaggggat gtactagatg
    19021 cgattgtgga aaaccttcca aatgaatatg aagaagaaaa tccagatgtc attaagttta
    19081 gcctgattgg tcgtcctaac gttggaaaat caagcttgat caatgctatc ttgggagaag
    19141 accgtgtcat tgccagtcct gttgctggaa caactcgtga tgccattgat acccacttta
    19201 cagatacaga tggtcaagag tttaccatga ttgatacggc tggtatgcgt aagtctggta
    19261 aggtttatga aaataccgag aaatactctg tcatgcgtgc catgcgtgct attgaccgtt
    19321 cagatgtggt cttaatggtc atcaatgcgg aagaggggat tcgtgaatac gacaagcgta
    19381 tcgctggatt tgctcatgaa gctggtaaag ggatgattat cgtggtcaac aagtgggata
    19441 cgcttgaaaa agataaccac actatgaaaa actgggaaga agatatccgt gagcagttcc
    19501 aatacctgcc ttacgcaccg attatctttg tatccgcttt gaccaagcaa cgtctccaca
    19561 aacttcctga gatgattaag caaatcagcg aaagtcaaaa tacacgtatt ccatcagctg
    19621 tcttgaacga tgtcatcatg gatgccattg ccatcaaccc aacaccgaca gacaaaggaa
    19681 aacgtctcaa gattttctat gcgacccaag tggcaaccaa accaccaacc tttgtcatct
    19741 ttgtcaatga agaagaactc atgcactttt cttacctgcg tttcttggaa aatcaaatcc
    19801 gcaaggcctt tgtttttgag ggaacaccga ttcatctcat cgcaagaaaa cgcaaataaa
    19861 aaagtagaat ctggaatgac agttccggat ttttttgata taataaaata atagaaaacg
    19921 ctatcaaaag aaggagggtg atgtcggtga tggaacattt gtttaaattc ttacttttag
    19981 caccgtactt ttattttgat aactggattg agaaggccaa cagaaatagt aagttttttc
    20041 caatttttta ttatttttac tggttttaca tccccttcta ttctcttttt agccttgctt
    20101 ggacagttgt ttcagttctg tttttcaata ccgtcttgag aaatgtgaca gatatcaagt
    20161 tatggggcat ttggtttctt tttattctgc tagctattgg tatgaattgg ttaacttatt
    20221 cctgtttcaa agaaatgttt tgcttgagac aggaactagg gaagtctaag ggtggaaggc
    20281 attgatttat atcggtttct atttgtagga ggtggcttat ggtacctcta aaatcattta
    20341 ttacacgcta ttccaaggtt tatattggta ataacattta atagaacgaa actaaagaaa
    20401 acaatttaaa aaaggaggct tgtgatggaa cacttgttta aaatgataat cttattaccc
    20461 tgtttttact ttttcagctg gattgacaag gataatagag aaagtaaatt tttcccaatt
    20521 ttttactatt tttactggat ttacatcacc ctctatgctc tttttagcct tgcttggaca
    20581 gttttttcag ttctgttttt caatatcgtc ttgagaaatt tgacagatat caagttatgg
    20641 ggtatttggc tattattact acttatcgct tttgctagtg actggttagc ctatgtcttt
    20701 ttcaaaaaaa tgcttgactt gagacgggaa ctagggaagt ctaaaggcgg aaggcattga
    20761 tttatactct tcgaaaatct cttcaaacca cgtcaacgtc gccttggatt atatatgtaa
    20821 ctgacttcgt cagtcttatc tacaacctca aagcagtgct ttgagcaact tgcggctagt
    20881 ttcctagttt gctctttgat tttcattgag tattatatta ctttctattt gtaggaggtg
    20941 gcttatgaag attcctctct taactttggc aaggcataaa tttgtttatg tcttgcttac
    21001 tttgcttttt cttgctttgg tttatcgtga cgttttgatg acttatttct tttttgatat
    21061 tcatgcgccc gatctagcta aattcgatgg acaagcaatt aaaaatgact tattaaaatc
    21121 agcattagat tttcgtattc tccagttcaa tctaggtttt tatcaatcat ttattattcc
    21181 aatcatcatt gttttgctag gttttcaata tattgagctg aaaaataaag ttttacgatt
    21241 gagtattgga agagaagtga gctatccagg gttaaaaaga aagttgactt tgcaagttgc
    21301 aagtatccct tgtttgatat atttagtgac tgtgctgaca attgcaatta taacctattt
    21361 ccttgggact ttttctcctc ttggatggaa ttctctattt tctgatggaa gtggtttaca
    21421 aagactccta gatggagaga taaaaagcta tttgttcttt acttgtgtcc tgctaatcgg
    21481 tattttcatc aatgcaatct attttttaca aatagttgat tatgtgggga atgtgactcg
    21541 ttcggcaatc acctatttga tgtttctttg gcttggttct atgctgctct atagtgcctt
    21601 gccttactat atggttccta tgacgagttt gatgcaagct agctatgggg atgtaagttt
    21661 gatgaaactc tttactcctt atatccttta tattgtccct tacatggtgc ttgaaaaata
    21721 tgaagataat gtttaagaat tttaacaata ttttgctaaa gagaaagatt gttttactac
    21781 ttcgtatagt tctgatgatg attttgataa accatctatt gtcaacagcg gttcaaaagc
    21841 aggatgctgt tatctttttc aagagagaat tgatttcaat tttttcctat aatgactatt
    21901 ctgaagcgaa tttagaaatc cccaaactct tgttaaacct ttcgcttttc atggtaggat
    21961 ggctctctgt cattttactt gaaagtgatt tggcagacca ttaccatcac ttgattcgct
    22021 atcaatcaag ctcctttttc gattatacaa ggaaacgatt ggttgtcatt tctaaatttt
    22081 ttactcaaga tttgtttgtc tggttccttg gtttacttcc gctaggaatt catttcaaaa
    22141 cagtcgcact tttcttttta cttgctcagt taatgatgtt gtacttacta ctgtcttatc
    22201 tgatagcact gattagtgcg ggcgctggtt tttccttttt tctctatttt ttagtatttg
    22261 tgggacaaga atggatgatg gatcatattg taacagtgta tttagtactc ttaagtttat
    22321 tagttatgtt gattgttagt cgcttggaag agaaatttaa gaaaggataa acgatgagac
    22381 ttgaaattat aaatggacag aaaatttatg ggaaaagacc tattttaaat cagttgaatt
    22441 tggtgtttca atcaggaaaa atttatggac ttaaaggtga taatggatct ggcaagacgg
    22501 ttcttttaaa ggtacttgct ggctatatta agcttgacaa aggaaaagtt cttcaagacg
    22561 ggaaagtcta cgggataaaa aatcattata ttcaggatgc aggaatttta attgaaaaag
    22621 tcgagttttt atctcattta tccctgagag aaaatttgga actgttaagg tatttttcat
    22681 ctaaagttac ggaaaaaaga attgcctatt ggattcaata ctatgattta caggaatttg
    22741 aagacgttga ataccgtcat ttatccttgg gaacaaagca aaaaatggcc ttgattcaag
    22801 cctttatttc ctctccttct atactctttc tcgatgaacc tatgaatgct ttggatgaga
    22861 agagtgtgag gttaaccaaa caggtcattt tatcttacct gaaaaaagaa aatggtctgg
    22921 ttatcctgac gtcgcacata tcggaagata tttcagacct ttgtacagat gtattagttg
    22981 tcgaaaatgg acatatacaa atgtaaagga tatacaatcc taggagatgg cttatggcac
    23041 atctaaaatc atttattaca cgctattcca aggtttatat tggtttagtt ctgctgatct
    23101 ggctgtcttt cttctttatc ccttgggata gagcgattct gggggtaagg attgacatct
    23161 tcatcataca gaaaatcttg ctagcttttg gaattctgtc cattctcatg gccttgctgt
    23221 ccaagaaagt cagtctcttt gtttttggac tgatttgctg tctttctctt tggattaact
    23281 tatttatcac atttgccatt ttgccgattt ttggcaatta aacagtcata aaagtcggag
    23341 aggttagctt gaaaactaac ctctttttcc ttttcaaaat ggggattctt ccttgaaaat
    23401 aatcagtaat tgtgctaaaa ttaaaggaac attctaaaat attcggaatt taaagtaagg
    23461 aaaaacatgg ctaatatttt aaaaacaatt atcgaaaatg ataaaggaga aatccgtcgt
    23521 ctggaaaaga tggctgacaa ggttttcaaa tacgaagacc aaatggctgc tttgactgac
    23581 gaccaactaa aagcaaaaac agttgaattt aaggaacgtt atcaaaatgg agaatcactg
    23641 gattcattgc tttacgaagc atttgcggtt gtccgtgaag gtgccaaacg tgtcctaggt
    23701 ctcttccctt ataaggttca ggtcatgggg gggattgttc ttcaccatgg tgacgtgcca
    23761 gagatgcgta caggggaagg gaaaacctta actgcgacca tgccggtata cctcaatgcc
    23821 ctttcaggta aaggggttca cgtagttacg gttaatgaat acctgtcaga acgtgacgcg
    23881 actgagatgg gtgaattgta ctcttggctt ggtttgtcag tagggattaa cttggctacc
    23941 aaatctccaa tggagaaaaa agaagcctat gagtgtgata ttacttactc aactaactca
    24001 gaaatcggat ttgactacct tcgtgacaac atggtcgttc gcgctgaaaa catggtacaa
    24061 cgtccgctta actatgcctt ggtcgatgag gttgactcta tcttgattga cgaggctcgt
    24121 acacctttga ttgtatcagg tgccaatgcg gttgaaacca gtcagttgta tcacatggca
    24181 gaccactatg taaaatcttt gaacaaagat gactacatca tcgatgtgca gtctaagact
    24241 attggtttgt ctgattcagg gattgacagg gctgaaagct acttcaaact tgaaaacctc
    24301 tatgacatcg aaaacgtggc tttgacccac tttatcgata acgcccttcg tgccaactac
    24361 atcatgcttc tcgatattga ctatgtggtg agcgaagagc aagaaatctt gattgtcgac
    24421 caatttacag gtcgtaccat ggaaggtcgt cgttattctg atggattgca ccaagctatt
    24481 gaagccaaag aaggtgtgcc aatccaggat gaaaccaaga catctgcctc aatcacgtac
    24541 caaaaccttt tccgtatgta caaaaaattg tctggtatga cgggtacagg taagactgag
    24601 gaagaagaat ttcgtgaaat ctacaacatt cgtgttattc caatcccaac aaaccgtcct
    24661 gttcaacgta ttgaccactc agaccttctt tatgcaagta tcgaatctaa gtttaaagcg
    24721 gttgtcgaag acgttaaggc tcgttaccaa aagggtcaac ctgtcttggt tggtacagta
    24781 gcggttgaaa ctagtgacta catttctaag aaattggttg cagctggtgt tcctcacgaa
    24841 gtcttgaatg ccaaaaacca ctatagagaa gcccaaatca tcatgaatgc tggtcaacgt
    24901 ggtgccgtta ccatcgcaac caacatggcg ggtcgtggta ccgacatcaa gcttggtgaa
    24961 ggtgttcgtg aacttggagg actttgtgtt attggtacag aacgtcatga aagtcgtcgt
    25021 atcgataacc agcttcgtgg acgttcaggt cgtcaaggag atccaggtga gtcacaattc
    25081 tacctatctc ttgaagatga tttgatgaaa cgttttggtt ctgaacgctt gaagggaatc
    25141 tttgaacgct tgaacatgtc tgaagaggcc attgagtctc gcatgttgac gcgtcaggtt
    25201 gaagcagctc agaaacgtgt cgaaggaaat aactacgata cccgtaaaca agtccttcaa
    25261 tacgacgatg tcatgcgtga acaacgtgag attatctata ctcaacgtta tgatgtcatt
    25321 actgcagacc gtgacttggc acctgaaatt caggcaatga tcaaacgcac gattggtcgt
    25381 gtcgttgacg gtcatgcgcg tgctaaacaa gatgagaaac tagaggcaat tttgaacttc
    25441 gctaagtaca acttgcttcc tgaagattct attacgatgg aagacttgtc aggcttgtct
    25501 gataaggcca tcaaggaaga gcttttccaa cgtgccttga aggtttacga tagtcaggtt
    25561 tcaaaactac gcgatgaaga agcagttaaa gaattccaaa aagttttgat tctacgagtg
    25621 gtggataaca agtggacaga tcatatcgat gcccttgatc aattgcgtaa cgcggttgga
    25681 cttcgtggct atgctcagaa caaccctgtt gttgagtatc aggcagaagg tttccgtatg
    25741 tttaatgata tgattggttc gattgagttt gatgtgacac gcttgatgat gaaagcacaa
    25801 attcatgaac aagaaagacc acaggcagaa cgtcatatca gtacaacagc gactcgcaat
    25861 atcgctgctc accaagcaag tatgccagaa gatttggatt tgagccagat tggacgcaat
    25921 gaactttgcc catgtggttc tggtaagaaa tttaaaaact gtcacggtaa aagacaataa
    25981 aatgagatag tttagaggcg gatatcttgt gaaaagtaaa tttttactgg gtatccgttt
    26041 gctttataag gagatgagtt atggtattta cagcaaaaag ctctaaaata aatatagaag
    26101 aagttcgtgc cttgtcaaaa ttagaaggtc aggctttgga gaggaaatca cagcgagatc
    26161 aagagctaga agccattata cgtggagaag accagcgaat tctcttggta atcgggccat
    26221 gctcatctga caacgaagaa gctgtccttg aatacgctaa gcgtttggca gtcctacaag
    26281 aagaagtggc agatcgtatc tttatggtta tgcgtgttta tactgccaaa ccccgtacca
    26341 acggagatgg ctataagggc ttgattcacc agcctaacgc gacagaagcg cctagtctta
    26401 tcaatggaat caaagccgtt cgccatcttc actatcgtgt catcacagaa acagggatga
    26461 caactgctga tgaaatgctt tatcctgaaa accttccgct tgtagatgat ttgatttctt
    26521 acatggcagt tggtgcccgt tcagttgaag accagcaaca ccgctttgtg gcaagtgggg
    26581 caggattttc tactggtttt aaaaatccaa cctctggaaa tctcaatgtc atgtttaatg
    26641 ggatttatgc tgctcaaaac aaacaaagtt tccttttctt aggaaaagaa gtagaaacaa
    26701 ctgggaaccc gctttcacac gctattcttc gtggtgctct taatgagtat ggaaaaaata
    26761 ttcccaacta ctattatgac aatttaattg ataccattgc ccagtatgag aaaatgggct
    26821 tggaaaatcc ttttatcatc attgatacca atcatgacaa ttctggtaag cagtatattg
    26881 aacagatccg aattgtccgc cagaccttga ttaaccgtgc ttggaatgaa aaaattaagc
    26941 agttcgttcg tggttttatg attgagtctt atctggaaga tggtcgacaa aatgagccag
    27001 aagtatttgg taagtctatc acagaccctt gcctgggttg ggataacaca gaagctcttg
    27061 tcagagaaat ttacaaaacg ttaggagaat aagatggcat ttattgaaaa aggtcaagaa
    27121 atcgatatgg aagtcatcaa ggctgaaacc caattgtctg cagaagcctt gagactcaag
    27181 gaaagccgtg acagggaatt ggcagatatt atttcagggg aagatgaccg tattctcttg
    27241 gtgattggtc cttgctcttc tgataatgaa gaggcggtct tggaatatgc tcgccgttta
    27301 tccgccttgc aaaagaaggt agcggacaag attttcatgg tcatgcgcgt gtatactgct
    27361 aagcctcgta ccaatggaga cggctataaa ggattggttc accagccaga tacttctaag
    27421 gctccaagcc tgattaacgg cttgcaggct gtgcgccagt tgcactaccg cgtgattaca
    27481 gagactggtt tgacaacggc agatgagatg ctttatccgt caaatctgat cttggtggat
    27541 gacttggtca gctaccatgc cgttggagct cgttctgtgg aagaccaaga gcaccgcttt
    27601 gtggcttctg ggattgatgc accagtaggg atgaaaaatc caacctcagg aaatttgggt
    27661 gttatgttta acgccatcta tgctgctcaa aacaagcaaa ccttccttta tcatgggcag
    27721 gaagttgaga catcaggtaa tcctttggcc catgttatcc tccgtggagc agtcaacgag
    27781 tatggcaatt atatgccgaa ttactactat gaaaacctac tccaagccat tgaacgctat
    27841 gaaaccatgg gacttgaaaa tccttttatc ctcattgaca ccaaccatga taactcaggc
    27901 aagcaatata tggagcagat tcgaattgtt cgccagacct tgcagaatcg tgattggaat
    27961 gagaaaatta aaaagacggt tcgaggattt atgattgaat cttacctagc agatggtcgt
    28021 caaaaccaac cagagatctt tggttgctct attactgacc cttgcctagg ttgggaaaat
    28081 acagaggcct tggtagaaga gatttatgtt accttgacaa aataagtgaa aaggatggag
    28141 ttggggaatc tcaactcctt ttgatgagaa tgatagttgg acacggaatt gacatcgaag
    28201 aattggcttc gatagaaagc gcagttacac gacatgaagg atttgctaag cgtgtactga
    28261 ccgctcagga aatggagcgc ttcaccagtc tcaaaggacg caggcaaata gaatatttag
    28321 ctggtcgctg gtcggctaag gaggcctttt ccaaggctat gggaacgggc attagcaagc
    28381 tcggttttca ggatttggaa gtcttgaaca atgaacgtgg ggcgccttat tttagtcagg
    28441 caccattttc aggaaagatt tggctgtcta tcagccacac cgatcagttt gtgacagcca
    28501 gtgtcatttt ggaggaaaat catgaaagct agtccacata gaccaaccaa ggctctgatt
    28561 tatctgggag ctattcgaca aaatattcag caaatggggg ctcatatccc tcaaggaacg
    28621 ctcaagttgg ctgtggttaa ggccaatgct tatggtcatg gagctgttgc cgttgccaag
    28681 gcaattcaag atgatgttga tggcttttgc gtttccaata tcgatgaagc cattgaactc
    28741 agacaagctg gactcagcaa gccaatcctc attttaggag tttctgaaat cgaagctgtt
    28801 gctctagcta aagaatatga cttcaccttg acagtggctg gactggagtg gattcaagca
    28861 ctcttagata aggaagtgga cctaactgga ttgacagtcc acctcaagat tgattcaggg
    28921 atgggacgga ttggttttag agaggccagt gaggttgagc aggctcaaga tttgctccaa
    28981 caacacggtg ttcgtgttga aggaatcttt acccactttg ctactgctga tgaggaatca
    29041 gatgactatt ttaatgccca gttagaacgg tttaaaacta ttttagctag tatgaaggaa
    29101 gttccagagc tggttcatgc tagcaattct gcaacgactc tttggcatgt agagactatt
    29161 ttcaatgcgg ttcgtatggg agatgccatg tatggcctca atccaagtgg agcggtcttg
    29221 gatttgcctt atgatttgat accggccttg accttggagt ctgctctggt tcatgtcaag
    29281 acagttccag ctggagcttg catgggctat ggagcaactt atcaagcgga tagcgagcaa
    29341 gtcatcgcga ccgtgccaat cgggtatgca gatggatgga caagagacat gcaaaatttc
    29401 tctgtcttgg tagatggcca agcttgccca attgtcggca gggtttcgat ggaccaaatc
    29461 actattcgat tgcctaagcc ttacccactc ggaaccaagg taaccttgat tggctccaat
    29521 ggggataagg aaatcactgc aactcaggta gcgacctacc gcgtaaccat taactatgag
    29581 gtggtttgcc tcctcagcga ccgtattccg agagaatatt attagaaaag aaaggagtgg
    29641 agcatgaatc tacatcaacc cttgcatgtc ttgcctggtg tgggaccaaa gtcagcagaa
    29701 aaatacgcca aactaggaat tgaaaacttg caagatctct tgctctactt tcctttccgt
    29761 tatgaagact tcaaaaccaa gcaggtgctg gagctggaag acggtgagaa ggcagttctt
    29821 tctggtcagg tagtgactcc tgctagtgtc cagtattatg gtttcaagcg caatcgcctg
    29881 cgttttagtc tcaagcaggg agaggtcgtt tttgcggtga atttctttaa ccagccctat
    29941 ctggctgata aaatagagtt gggagcaacc cttgctgtct ttggaaaatg ggaccgcgct
    30001 aaggctagtc tgactgggat gaaggttctg gctcaggtag aagatgacct ccagcctgtc
    30061 tatcgtctgg ctcagggaat cagtcaggcc agtctggtta aggtcatcaa gacggctttt
    30121 gatcagggac tggacctctt gatagaagaa aatctgcccc agtctttact agacaaatac
    30181 aaactcatgt cccgttgtca ggcagtccga gctatgcatt ttccaaagga tttggcagaa
    30241 tacaagcagg ctcttcgccg tatcaagttt gaggaactct tttatttcca aatgcagttg
    30301 cagacgctca agtctgaaaa tagagttcag ggaagtggtc tggttctgaa ttggtctcag
    30361 gaaaaagtga cagcagttaa agtaagtctt ccttttgccc tgacccaagc tcaggaaaag
    30421 agtttgcagg aaattttaac tgatatgaag tccgaccacc acatgaatcg tctcctacaa
    30481 ggggatgtgg ggagtggaaa aacggtagtc gctggcttgg ccatgtttgc ggcagtgaca
    30541 gcaggttatc aggctgccct aatggtacca acagaaatcc tcgcagagca acactttgag
    30601 agtttacaga acctttttcc caatttgaaa ctggctctct tgacaggttc cttgaaagct
    30661 gcagaaaaga gagaagtctt ggagaccatt gccaagggtg aggctgattt gattatcgga
    30721 actcacgctc tgatacaaga tggggtggag tatgctcgtc ttggtttgat tattatcgat
    30781 gagcagcacc gttttggtgt agggcaaagg cgtattttac gggaaaaagg tgacaatcca
    30841 gatgtcctca tgatgacggc gactcccatt ccacggacgc ttgccatcac agcctttgga
    30901 gatatggatg tttccattat cgaccagatg ccagcaggtc ggaagcctat tgtgacgcgc
    30961 tggatcaaac atgagcaact acctcaggtc ttgacttggt tagaggggga aattcaaaaa
    31021 ggttcccaag cctatgtcat ctctcctttg attgaagaat cagaagctct agatttgaaa
    31081 aatgccattg ccttatcaga ggagttgacg actcattttg caggcaaggc agaggtggct
    31141 cttctacatg gtaggatgaa gagtgacgaa aaagaccaga tcatgcagga tttcaaggag
    31201 agaaagacgg atattctggt ttcgacgacg gttattgagg ttggggtcaa cgttcccaat
    31261 gcgactgtca tgattatcat ggatgccgat cgcttcggtc tcagccagct tcaccagctt
    31321 agaggtcgtg tcggtcgggg ggacaagcag tcctacgctg ttctcgttgc caatcccaag
    31381 acggattctg ggaaagaccg catgcgcatc atgacagaaa cgaccaatgg atttgtcctt
    31441 gcggaggaag atttgaaaat gcgtggttct ggtgagattt ttggaaccag acagtcagga
    31501 cttccagagt tccaagtggc tgatattatc gaagattttc cgattttaga agaagcaaga
    31561 aaggttgcta gctacattag ttctatagaa gcttggcaag aagatccaga gtggcgcatg
    31621 attgcccttc atctggaaaa gaaagaacat ctggattaag ctttctctaa ggaaaactta
    31681 tactcaatga aaatcaaaga gcaaactagg aagctaaccg caggttgctc aaaacactgt
    31741 tttgaggttg tggatgaaac tgacgaagtc agctcaaaac accgttttga ggtggcagat
    31801 agaactgacg aagtcagtaa catatatata cggtaaggcg acgctgacgt ggtttgaaga
    31861 gattttcgaa gagtattaag ctagttttta ggtttggctc ttatactaaa gtcatcaaaa
    31921 agaaacgagg actctcatat gacagtaacg attaaagtaa attaccaaac cactttccaa
    31981 aagaaggaag caaaaaacta gtataaacag aagagagagc gaaatgctct tttttcgttt
    32041 ctaaaactac tttcagccca tcatcctaaa agtaaagaat ctaaattcac tttctattta
    32101 cccttctttc ttgcattgat tacatagata tgctacagtt gtggtaacga ttacaaaata
    32161 aaaggagcat gctatgaaaa atccagcttt gctagaagaa attaagacct atagaggaag
    32221 ggatgaggtt ccggaagact ttgatgcttt ctgggatggg gaagtgaaaa atgtttccac
    32281 gcttccatcc taccacttgg aggaaagaga tttccacatt cctcaagtca agtgctatga
    32341 gttaacattt gaaggaagca aggaaggaaa ggtctatgca cgcattgttc ttccaaagag
    32401 tgaggagaag gttccactaa tcttccattt tcatggttat atgggacgtg gctgggactg
    32461 ggccgacatg ctgggcttca ccgtagctgg ttacggtgtt gtttccatgg atgtgcgggg
    32521 ccagtcaggt tactcacaag acggcttgcg ttctccttta ggaaataccg tgaaggggca
    32581 tattatccgt ggtgctgtgg aaggtcggga ccacctcttt tataaggatg tttatctgga
    32641 tatttaccag ttggtcgaaa ttgttgctag tctgtctcag gttgatgaga agcgtctttc
    32701 tagctatggt gcctcacaag gaggggctct agccctagtt gcagcagccc tcaatcctcg
    32761 aattcagaaa acagttgcca tttatccctt cttgtcagac ttcagacggg tgattgagat
    32821 tggtaatact agcgaggctt acgacgaact tttccgttat ttcaagtttc acgacccctt
    32881 ccatgaaaca gaggaggaaa tcatggcgac ccttgcctat atcgatgtca aaaatcttgc
    32941 ccatcgtatc caaggtgagg ttaagatgat tacgggcttg gacgacgatg tttgctatcc
    33001 cattacccag tttgcgattt ataatcgtct gacctgcgat aaaacctatc gtatcatgcc
    33061 tgagtatgct cacgaagcca tgaatgtatt tgtcaatgac caagtctaca actggctctg
    33121 tggaagtgag attcctttta aatatctaaa ataaggagtc gactctaagt acaaaatctt
    33181 aaaaattaca aacacgcata gtatcagggg attaagaaaa cttgatacta tgcgttttat
    33241 catagaaata tagtaaaatg aaacaagaac aggacaaatt gatcaggaca gtcaaatcga
    33301 tttctaacaa tgttttagaa acaaaggtgt actattctag tttcaatcta ttatatttat
    33361 agaatttttt gttgctagat ttgtcaaatt gcttaaaata atttttttca gaaagcaaaa
    33421 gccgatacct atcgagtagg gtagttcttg ctatcgtcag gcttgtctgt aagtgttaat
    33481 acttttcaaa agtctcttca aaccacgtca gcttcgcctt gccgtatata tgttactgac
    33541 ttcgtcagtt ctatctgcca cctcaaaacg gtgttttgag ctgactttgt cagtcttatc
    33601 tacaacctca aaacagtgtt ttgagcatca tgcggctagc ttcttagttt gctctttgat
    33661 tttcattgag tataaaaaca gatgagtttc tgttttcttt ttatggatta taaatgttca
    33721 gctgaaacta ctttcaagga cattattata taaaagaatt ttttgaaact aaaatctact
    33781 atattacact atattgaaag cgttttaaaa atgaggtata ataaatttac taacacttat
    33841 aaaaagtgat agaatctatc tttatgtata tttaaagata gattgctgta aaaatagtag
    33901 tagctatgcg aaataacaga tagagagaag ggattgaagc ttagaaaagg ggaataatat
    33961 gatatttaag gcattcaaga caaaaaagca gagaaaaaga caagttgaac tacttttgac
    34021 agtttttttc gacagttttc tgattgattt atttcttcac ttatttggga ttgtcccctt
    34081 taagctggat aagattctga ttgtgagctt gattatattt cccattattt ctacaagtat
    34141 ttatgcttat gaaaagctat ttgaaaaagt gttcgataag gattgagcag gaagtatggt
    34201 ataaatagca taagctgatg tccatcattt gcttataaag agatatttta gtttaattgc
    34261 agcggtgtcc tggtagataa actagattgg caggagtctg attggagaaa ggagagggga
    34321 aaattggcac caatttgaga tagtttgttt agttcatttt tgtcatttaa atgaactgta
    34381 gtaaaagaaa gttaataaaa gacaaactaa gtgcattttc tggagtaaat gtcttatttc
    34441 agaaatcggg atatagatat agagaggatc agtatgaatc ggagtgttca agaacgtaag
    34501 tgtcgttata gcattaggaa actatcggta ggagcggttt ctatgattgt aggagcagtg
    34561 gtatttggaa cgtctcctgt tttagctcaa gaaggggcaa gtgagcaacc tctggcaaat
    34621 gaaactcaac tttcggggga gagctcaacc ctaactgata cagaaaagag ccagccttct
    34681 tcagagactg aactttctgg caataagcaa gaacaagaaa ggaaagataa gcaagaagaa
    34741 aaaattccaa gagattacta tgcacgagat ttggaaaatg tcgaaacagt gatagaaaaa
    34801 gaagatgttg aaaccaatgc ttcaaatggt cagagagttg atttatcaag tgaactagat
    34861 aaactaaaga aacttgaaaa cgcaacagtt cacatggagt ttaagccaga tgccaaggct
    34921 ccagcattct ataatctctt ttctgtgtca agtgctacta aaaaagatga gtacttcact
    34981 atggcagttt acaataatac tgctactcta gaggggcgtg gttcggatgg gaaacagttt
    35041 tacaataatt acaacgatgc acccttaaaa gttaaaccag gtcagtggaa ttctgtgact
    35101 ttcacagttg aaaaaccgac accagaacta cctaaaggcc gagtgcgcct ctacgtaaac
    35161 ggggtattat ctcgaacaag tctgaaatct ggcaatttca ttaaagatat gccagatgta
    35221 acgcatgtac aaatcggagc aaccaagcgt gccaacaata cggtttgggg gtcaaatcta
    35281 cagattcgga atctcactgt gtataatcgt gctttaacac cagaagaggt acaaaaacgt
    35341 agtcaacttt ttaaacgctc agatttagaa aaaaaactac ctgaaggagc ggctttaaca
    35401 gagaaaacgg acatattcga aagcgggcgt aacggtaacc caaataaaga tggaatcaag
    35461 agttatcgta ttccagcact tctcaagaca gataaaggaa ctttgatcgc aggtgcagat
    35521 gaacgccgtc tccattcgag tgactggggt gatatcggta tggtcatcag acgtagtgaa
    35581 gataatggta aaacttgggg tgaccgagta accattacca acttacgtga caatccaaaa
    35641 gcttctgacc catcgatcgg ttcaccagtg aatatcgata tggtgttggt tcaagatcct
    35701 gaaaccaaac gaatcttttc tatctatgac atgttcccag aagggaaggg aatctttgga
    35761 atgtcttcac aaaaagaaga agcctacaaa aaaatcgatg gaaaaaccta tcaaatcctc
    35821 taccgtgaag gagaaaaggg agcttatacc attcgagaaa atggtactgt ctatacacca
    35881 gatggtaagg cgacagacta tcgcgttgtt gtagatcctg ttaaaccagc ctatagcgac
    35941 aagggtgatc tatacaaggg taaccaatta ctaggcaata tctacttcac aacaaacaaa
    36001 acttctccat ttagaattgc caaggatagc tatctatgga tgtcctacag tgatgacgac
    36061 gggaagacat ggtcagcgcc tcaagatatt actccgatgg tcaaagccga ttggatgaaa
    36121 ttcttgggtg taggtcctgg aacaggaatt gtacttcgga atgggcctca caagggacgg
    36181 attttgatac cagtttatac gactaataat gtatctcact taaatggctc gcaatcttct
    36241 cgtgtcatct attcagatga tcatggaaaa acttggcatg ctggagaagc ggtcaacgat
    36301 aaccgtcagg tagacggtca aaagatccac tcttctacga tgaacaatag acgtgcgcaa
    36361 aatacagaat caacggtggt acaactaaac aatggagatg ttaaactctt tatgcgtggt
    36421 ttgactggag atcttcaggt tgctacaagt aaagacggag gagtgacttg ggagaaggat
    36481 atcaaacgtt atccacaggt taaagatgtc tatgttcaaa tgtctgctat ccatacgatg
    36541 cataatggaa aagaatacat tatcctcagc aatgcaggtg gaccgaatcg tgaaaatggg
    36601 atggtccact tggcacgtgt cgaagaaaat ggtgagttga cttggctcaa acacaatcca
    36661 attcaaaaag gagagtttgc ctataattcg ctccaagaat taggaaatgg ggagtatggc
    36721 atcttgtatg aacatactga aaaaggacaa aatgcctata ccctatcatt tagaaaattt
    36781 aattgggaat ttttgagcaa agatctgatt tttcctaccg aagcgaaagt gaagcgaact
    36841 agagagatgg gcaaaggaga gatgggcaaa ggagttattg gcttggagtt cgactcagaa
    36901 gtattggtca acaaggctcc aacccttcaa ttggcaaatg gtaaaacagc gactttccta
    36961 acccagtatg atagcaagac cttgttgttt gcagtagata aggaagatat cggacaggaa
    37021 attattggta tagctaaagg aagcatcgaa agtatgcata atcttcctgt aaatctagca
    37081 ggtgccagag ttcctggcgg agtaaatggt aacaaagcag cggtgcatga agttccagaa
    37141 tttacagggg gagttaatgg tacagagcca gctgttcatg aaatcgcaga gtataaggga
    37201 tctgattcgc ttgtaactct tactacaaaa gaagattata cttacaaagc tcctcttgct
    37261 cagcaggcac ttcctgaaac agggaacaag gagagtgacc tcctagcttc actaggacta
    37321 acagctttct tccttggtct gtttacgcta gggaaaaaga gagaacaata agagaagaat
    37381 tctaaacatt tgattttgta aaaatagaag gagatagcag gttttcaagc ctgctatctt
    37441 tttttgatga cattcaagct gatacgaaat cataagaggt ctgaaactac tttcagagta
    37501 gtctgttcta taaaatatag tagattgaaa taagatgtga acaactctat caggaaagtc
    37561 aaattaattt atagaaaaat tttagcagtc aaggtgtact gttatagatt caatatatta
    37621 tgtgactatt aaccttctct tctcctaaaa ttgactttct tgttttctta tcttgtccac
    37681 tcgaaacaag tattgtaaga atttgattat ttttgaaagt acttttaata tacttgatat
    37741 agttaaaaaa gatttgaaac taaattccaa attagaaaaa gacttgaaat actaaaaaaa
    37801 aaaagtatac tctaattgaa aacggtaaca aaactaattt agagaatgaa atatagagta
    37861 tttctctctt aaaagttttt ggtgaaacga gatgtagaaa ggagatttag ccaaagagtc
    37921 tattagtgct agaataatag attagaatta ttttagaaaa acgaagtgag cagcttataa
    37981 attcaagtcc ccaaatagat tcatactagt atcttttgca aaaaataaag ggcgacttcc
    38041 ttcatgaata tcaatttcat ctataaggaa ggtagctaat tgaactaact tatttattct
    38101 gtttgtcgct agaaaaatca gacctccttg tgaagattga ggagatactt aatgaaaatc
    38161 aaagaagaaa ctagcaagct agtagcagat tgcccaaaac accgctttga ggttgtagat
    38221 aagactgacc tatataatcc aaggtgaagc gactgtggtt tgaagagatt ttcaaagagt
    38281 ataggctaga gagtagtgtt tttatgtcct tctagtagaa aatgctagac agaagaatgg
    38341 ggaacttgga taggaaaaat agattgagaa aggaggttag aagagatgat tattacaaaa
    38401 attagccgtt taggaactta tgtgggagta aatccacatt ttgcaacatt aatagatttt
    38461 ctagaaaaaa caggactaga aaatttaaca gaaggttcga ttgctatcga tggtaatcga
    38521 ttgtttggga attgctttac ttatctagca gatggtcaag caggggcttt ctttgaaaca
    38581 caccaaaaat atttggatat tcatttagtt ttggaaaacg aagaagccat ggctgttaca
    38641 tcgccggaaa atgtaagcgt tacccaagaa tatgatgaag agaaagatat tgaattatac
    38701 acagggaaag tggaacagtt ggttcatttg agagctggcg aatgcctcat cacttttcca
    38761 gaagatttac atcaacccaa ggttcgtata aatgatgaac ctgtgaaaaa agttgtcttt
    38821 aaagttgcga tttcttaatg tagaaagaga agaacgatga aaaaaatgag aaagttttta
    38881 tgtctagctg gaattgcgct agcggctgtt gccttggtag cttgttcagg aaaaaaagaa
    38941 gctacaacta gtactgaacc accaacagaa ttatctggtg agattacaat gtggcactcc
    39001 tttactcaag gaccccgttt agaaagtatt caaaaatcag cagatgcttt catgcaaaag
    39061 catccaaaaa cgaaaatcaa gattgaaaca ttttcttgga atgacttcta tactaaatgg
    39121 actacaggtt tagcaaatgg aaatgtgcca gatatcagta cagctcttcc taaccaagta
    39181 atggaaatgg tcaactcaga tgctttggtg ccactaaatg attctatcaa gcgtattgga
    39241 caagataaat ttaacgaaac tgccttaaat gaggcaaaaa tcggagatga ttactactct
    39301 gttcctcttt attcacatgc acaagtcatg tgggttagaa cagatttgtt aaaagaacat
    39361 aatattgagg ttcctaaaac ttgggatcaa ctctatgaag cttctaaaaa attgaaagaa
    39421 gctggagttt atggcttgtc tgttccgttt ggaacaaatg acttaatggc aacacgtttc
    39481 ttgaacttct acgtacgtag tggtggagga agcctcttaa caaaagatct taaagcagac
    39541 ttgacaagcc aacttgctca agatggtatt aaatactggg ttaaattgta taaagaaatc
    39601 tcacctcaag attctttgaa ctttaatgtc cttcaacaag ctaccttgtt ctatcaagga
    39661 aaaacagcat ttgactttaa ctctggcttc catatcggag gaattaatgc caacagtcct
    39721 caattgattg attcgattga tgcttatcct attccaaaaa tcaaagagtc tgataaagac
    39781 caaggaattg aaacctcaaa cattccaatg gttgtttgga aaaattcaaa acatccagaa
    39841 gttgctaaag cattcttaga agcactttat aatgaagaag actacgttaa attccttggt
    39901 tcaactccag taggtatgtt gccaactatt aaggggatta gcgattctgc agcctataaa
    39961 gaaaatgaaa ctcgtaagaa atttaaacat gctgaagaag taattactga agctgttaaa
    40021 aaaggtactg ctattggtta tgaaaatgga ccaagtgtac aagctggtat gttaactaac
    40081 caacacatta ttgaacaaat gttccaagat atcattacaa atggaacaga tcctatgaaa
    40141 gcagcaaaag aagcagaaaa acaattaaac gatttatttg aggctgttca gtagatgtaa
    40201 aagactagaa aataggtggg ctagtgatct gaaaagctct agcccaatct tgtaaaagaa
    40261 gggagaagga gaatggttaa agaacgtaat ttaactcgct ggatatttgt tttgccagct
    40321 atgattatcg taggattact ctttgtttat ccgtttttct cgagtatttt ttatagcttt
    40381 accaataagc atttgattat gcctaattat aaatttgttg gtttggctaa ctataaagct
    40441 gtgctatcag atcccaactt ctttaatgcg ttctttaatt caattaagtg gaccgttttc
    40501 tcattagttg gtcaagtttt agtagggttt gtattggctt tagctcttca cagagtacgc
    40561 cacttcaaga aattatatag gaccttattg attgttcctt gggcatttcc taccatcgtt
    40621 attgcctttt cttggcagtg gattctaaac ggggtttatg gctacttacc taatctaatc
    40681 gtaaaattag gtttaatgga acatacacct gcatttttga cagatagtac atgggcattc
    40741 ctatgtttgg tgtttatcaa catttggttt ggagcaccaa tgattatggt taatgtgctt
    40801 tcagctttgc aaacagtacc agaagaacaa tttgaggctg ctaagataga tggtgcttca
    40861 agttggcagg tgttcaagtt tatcgtcttt ccacatatta aagtggttgt aggacttcta
    40921 gttgttttga gaactgtatg gatctttaat aactttgaca ttatctacct cattactggt
    40981 ggtggaccag ccaatgctac aacgacgctt ccaatttttg cttacaacct gggctgggga
    41041 actaaattgt tgggtcgtgc ttcagcagtt acagtattgc tctttatctt cttggtggcg
    41101 atttgcttta tctactttgc tatcatcagt aagtgggaaa aggagggtag aaaataatga
    41161 agaagaaatc cagtatttat ttagatattc tctcacatgt acttttagtt ggtgcgacca
    41221 tcgttgcagt tttcccattg gtatggatta tcatatcttc tgtcaaaggg aaaggggaat
    41281 taactcagta tccaacacga ttttggcctg aacagtttac attagattat ttcactcatg
    41341 ttatcaacga tttgcacttc attgataaca ttcgaaacag tttaatcatt gccttggcta
    41401 caacccttat tgcgattatt atttctgcta tggcagccta tggtattgtt cgattctttc
    41461 ctaaattggg agcaatcatg tcgagactac tcgtcattac ctacattttc ccaccaattt
    41521 tgttagcaat tccctattca attgccattg ctaaagttgg gttaacaaat agtttatttg
    41581 gcttgatgat ggtttatcta tcttttagtg ttccatatgc agtttggctc ttagttggat
    41641 ttttccaaac agttccaatt ggaattgaag aagcggctag aattgatggt gcaaataaat
    41701 ttgttacgtt ttataaagtt gtgctaccga ttgtagcacc aggtattgta gcaacagcta
    41761 tttatacatt tatcaatgct tggaatgaat tcctgtatgc cttgattttg attaacaata
    41821 caggaaagat gacagtagca gtagcccttc gttcacttaa tggttcagaa atactagact
    41881 ggggagatat gatggcagcg tctgttattg tagttcttcc atcaattatt ttcttctcta
    41941 tcatccaaaa taagattgca agtggattat cagaaggatc tgtgaagtaa tactcaatga
    42001 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaagtacagc tttgaggttg
    42061 cagataaaac tgacgaagtc agtaaccata cgcacagtaa ggcgacgttg acgcggtttg
    42121 aagagatttt cgaagagtat aagttaaaat tagaaagttt attcaataat aaaggagaaa
    42181 aacatggtta aatacggtgt tgttggagca gggtattttg gagctgaatt ggctcgctac
    42241 atgcaaaaga atgatggagc agagattact cttctctatg atccagataa tgcagaggcg
    42301 attgcagaag aattgggagc aaaagtagca agttccttag atgagttggt ttctagcgat
    42361 gaagtagatt gtgttatcgt cgcaactcca aataatcttc ataaggaacc ggttattaag
    42421 gctgcacagc atggtaaaaa tgttttctgt gaaaaaccaa ttgcgctttc ttatcaagat
    42481 tgtcgcgaga tggtagatgc gtgtaaagaa aacaatgtaa cctttatggc aggacatatt
    42541 atgaatttct ttaatggtgt tcatcatgca aaagaactca ttaatcaagg agttatcgga
    42601 gacgttctat attgtcatac agctcgtaat ggttgggaag aacaacaacc gtcagtatca
    42661 tggaaaaaaa ttcgtgaaaa atcaggtggt cacttgtatc accacatcca tgaattggat
    42721 tgcgttcaat tccttatggg gggcatgcct gaaactgtaa ccatgacagg tggaaatgtg
    42781 gcccatgaag gtgaacattt cggtgatgaa gatgatatga tttttgtcaa tatggaattt
    42841 tctaataagc gttttgcttt gttagaatgg ggttcagctt atcgttgggg tgaacattat
    42901 gtcttaatcc aaggaagcaa aggtgccatc cgcttagact tattcaactg taaaggaact
    42961 cttaagctag atgggcaaga aagctatttc ttgattcacg aatcgcaaga agaagatgat
    43021 gatcggattc gtatctatca tagtacagag atggatggag caattgctta tggtaaacca
    43081 ggtaaacgta ctccattatg gctatcatct gtcattgata aagaaatgcg ctatctgcat
    43141 gagattatgg aaggagctcc agtatcagaa gaatttgcaa aacttttgac aggtgaagct
    43201 gccctagaag caattgctac tgcagatgct tgtacccagt ctatgtttga agatcgcaaa
    43261 gtaaaattgt cagaaattgt aaaataaatt ttggtattct cctatttata ggtcgacttg
    43321 ctcctctgaa agtactttta gaggagctgt ttgactttgc tagtttttga aactgaaatc
    43381 tattatacta caaactattg aaagcgtttt aattttaagg tataataatc tcatagaaat
    43441 aaagaaaagg aggaaagagg atgccacaga ttagcaaaga agccttgatt gagcaaatca
    43501 aagatggaat catcgtttct tgtcaggctc ttcctcatga accgctttat acagaagcgg
    43561 gaggggtgat tcccttgctg gtcaaagcgg ctgagcaagg tggagcagtc ggtatccgag
    43621 caaacagtgt tcgcgatatc aaggaaatta aggaagtcac taaacttcca atcattggga
    43681 ttatcaaacg tgattatcca cctcaggaac ccttcatcac ggctactatg aaagaagttg
    43741 atgaattggc agaactggac atcgaggtga ttgctctgga ttgtaccaag cgtgaacgct
    43801 acgatggttt ggaaattcaa gagttcattc gtcaggttaa ggagaaatat cctaatcagc
    43861 ttttgatggc tgatactagt atcttcgaag aagggctagc agctgtagaa gcaggaattg
    43921 actttgtcgg aacaacctta tcaggctaca catcctacag tccaaaagta gacggtccag
    43981 attttgaatt gattaagaaa ctctgtgatg ccggtgtaga tgtcattgca gaaggaaaaa
    44041 ttcatacacc agaacaagcc aaacaaatcc ttgaatatgg agtgcgaggc atcgttgttg
    44101 gtggcgccat tactagacca aaagagatta cagaacgctt cgttgctagt cttaaataag
    44161 atgtgagagg gagttttatg tttaaagttt tacaaaaagt tggaaaagct tttatgttac
    44221 ctatagctat acttcctgca gcaggtctac ttttggggat tggtggtgca ctttcaaacc
    44281 caaccacgat agcaacttat ccaatactag acaatagtat ttttcaatca atattccaag
    44341 taatgagctc tgcaggagag gttgtattca gtaatttgtc actacttctc tgtgtgggat
    44401 tatgtattgg cttagcgaaa cgagataaag gaaccgctgc gttagcagga gtaactggtt
    44461 acttagttat gactgcaacg atcaaagctt tggtaaaact ttttatggca gaaggatctg
    44521 caattgatac tggagttatt ggagcattag ttgtcggaat agttgccgta tatttgcaca
    44581 accgatataa caatattcaa ttaccttccg ctttaggatt ctttggaggt tcacgcttcg
    44641 ttcctattgt tacatcgttc tcttctatct tgattggctt tgtcttcttt gttatttggc
    44701 cacctttcca acaacttctt gtttctacag gtggatatat ttctcaggcg ggtccaattg
    44761 gaacttttct atatggattt ttaatgagac tttctggagc agtaggctta catcatataa
    44821 tttaccctat gttttggtat actgaacttg gtggtgttga aactgttgca ggacaaacag
    44881 tggttggagc tcaaaaaata ttttttgctc aattagccga tctggcccat tctggattat
    44941 ttacagaagg aacaaggttt tttgcaggtc gtttctcaac aatgatgttc ggtttaccgg
    45001 ctgcctgttt agcgatgtac catagtgttc ctaaaaatcg tcgtaaaaaa tacgcgggtt
    45061 tgttttttgg agttgcttta acatctttta ttaccggtat tacagaacca attgaattta
    45121 tgtttctatt cgtcagtccg gttctatatg ttgttcacgc attccttgat ggtgttagct
    45181 tctttattgc agacgtctta aatatttcaa taggaaacac attttcagga ggtgtaatcg
    45241 atttcacttt atttggaatt ttgcagggga acgctaagac gaattgggtt cttcagattc
    45301 catttggact tatttggagt gttttgtatt atattatttt tagatggttc attactcaat
    45361 tcaacgttct aacgccagga cgaggagaag aagtagattc taaagaaatt tctgaatccg
    45421 cagattcaac ttcaaatact gcagattatt taaaacagga tagcctacaa attatcagag
    45481 ccttgggtgg atcaaataat atagaagatg tagatgcttg tgtgacacgt ttacgtgtag
    45541 ctgtaaaaga agttaatcaa gttgataaag cacttttaaa acaaattggt gcagttgatg
    45601 tcttagaagt gaagggtggc attcaagcaa tctatggagc aaaagcaatc ttatataaaa
    45661 atagtattaa tgaaatttta ggtgtagatg attaagtact tactgactta ataaaaaaca
    45721 gaggagagtg atggatgagt aggatgaaat gaaatcgcat acaagaaata aagaactcat
    45781 tatccaagtt ggatacgctt attacatagg agaatacaaa tgaaatttag aaaattagct
    45841 tgtacagtac ttgcgggtgc tgcggttctt ggtcttgctg cttgtggcaa ttctggcgga
    45901 agtaaagatg ctgccaaatc aggtggtgac ggtgccaaaa cagaaattac ttggtgggca
    45961 ttcccagtat ttacccaaga aaaaactggt gacggtgttg gaacttatga aaaatcaatc
    46021 atcgaagcgt ttgaaaaagc aaacccagat ataaaagtga aattggaaac catcgacttc
    46081 aagtcaggtc ctgaaaaaat cacaacagcc atcgaagcag gaacagctcc agacgtactc
    46141 tttgatgcac caggacgtat catccaatac ggtaaaaacg gtaaattggc tgagttgaac
    46201 gacctcttca cagatgaatt tgttaaagat gtcaacaatg aaaacatcgt acaagcaagt
    46261 aaagctggag acaaggctta tatgtatccg attagttctg ccccattcta catggcaatg
    46321 aacaagaaaa tgttagaaga tgctggagta gcaaaccttg taaaagaagg ttggacaact
    46381 gatgattttg aaaaagtatt gaaagcactt aaagacaagg gttacacacc aggttcattg
    46441 ttcagttctg gtcaaggggg agaccaagga acacgtgcct ttatctctaa cctttatagc
    46501 ggttctgtaa cagatgaaaa agttagcaaa tatacaactg atgatcctaa attcgtcaaa
    46561 ggtcttgaaa aagcaactag ctggattaaa gacaatttga tcaataatgg ttcacaattt
    46621 gacggtgggg cagatatcca aaactttgcc aacggtcaaa catcttacac aatcctttgg
    46681 gcaccagctc aaaatggtat ccaagctaaa cttttagaag caagtaaggt agaagtggta
    46741 gaagtaccat tcccatcaga cgaaggtaag ccagctcttg agtaccttgt aaacgggttt
    46801 gcagtattca acaataaaga cgacaagaaa gtcgctgcat ctaagaaatt catccagttt
    46861 atcgcagatg acaaggagtg gggacctaaa gacgtagttc gtacaggtgc tttcccagtc
    46921 cgtacttcat ttggaaaact ttatgaagac aaacgcatgg aaacaatcag cggctggact
    46981 caatactact caccatacta caacactatt gatggatttg ctgaaatgag aacactttgg
    47041 ttcccaatgt tgcaatctgt atcaaatggt gacgaaaaac cagcagatgc tttgaaagcc
    47101 ttcactgaaa aagcgaacga aacaatcaaa aaagctatga aacaatagtc cttagttatt
    47161 ctataaaaag tagtttttta aagaacctaa gagtgtatac ccccttttcc ctctacacag
    47221 atagtgtaag aaaagggggc ttttgtttaa aatgtaagaa actgtcacga aattaaaatg
    47281 aagttcttac ataagcgaat cataaaaaat ttcattttga ttttaaaaca gttcaagaaa
    47341 gtcaaaaaat tattctattt gaaagagagg tgccgactgt gaaagtcaat aaaatccgta
    47401 tgcgggaaac agtgatttcc tacgctttcc tagcaccagt attattcttc tttgtcatct
    47461 ttgtgttggc tccgatggtg atgggcttca ttacaagttt ctttaactac tcaatgacta
    47521 aatttgagtt tgtaggcttg gataactata tccgtatgtt taaagatcct gtctttacaa
    47581 aatctctgat taacacagtt attttggtta ttggatctgt accagttgtt gttctattct
    47641 cactctttgt agcatctcag acctatcatc aaaatgtcat tgccagatcc ttctaccgtt
    47701 tcgtcttctt ccttcctgtt gtaacgggta gtgttgccgt gacagttgtt tggaaatgga
    47761 tttatgaccc gctatcaggg attctaaact ttgtccttaa gtcaagccac atcatcagcc
    47821 aaaacatttc ttggttggga gataaaaact gggcattgat ggcgattatg attattctct
    47881 tgaccacttc agttggtcag cccatcatcc tttatatcgc tgccatgggg aatattgaca
    47941 attcactggt tgaagcggcg cgtgttgatg gtgcaactga gtttcaagtt ttttggaaga
    48001 ttaaatggcc aagccttctt ccaacaactc tttatattgc aatcatcaca acaattaact
    48061 cattccagtg tttcgccttg attcagcttt tgacatctgg tggtccaaac tactcaacaa
    48121 gtaccttgat gtactacctt tacgaaaaag ccttccaatt gacagaatac ggctatgcca
    48181 acacaattgg tgtcttcttg gcagtcatga ttgctatcgt aagctttgtt caatttaaag
    48241 tacttggaaa cgacgtagaa tactaaagaa aggagacagc tatgcaatct acagaaaaaa
    48301 aaccattaac agcctttact gttatttcaa caatcatttt gctcttgttg actgtgctgt
    48361 tcatctttcc attctactgg attttgacag gggcattcaa atcacaacct gatacaattg
    48421 ttattcctcc tcagtggttc cctaaaatgc caaccatgga aaacttccaa caactcatgg
    48481 tgcagaaccc tgccttgcaa tggatgtgga actcagtatt tatctcattg gtaaccatgt
    48541 tcttagtttg tgcaacctca tctctagcag gttatgtatt ggctaaaaaa cgtttctatg
    48601 gtcaacgcat tctatttgct atctttatcg ctgctatggc gcttccaaaa caagttgtcc
    48661 ttgtaccatt ggtacgtatc gtcaacttca tgggaatcca cgatactctc tgggcagtta
    48721 tcttgccttt gattggatgg ccattcggtg tcttcctcat gaaacaattt agtgaaaata
    48781 ttcctacaga gttgcttgaa tcagctaaaa tcgacggttg tggtgagatt cgtaccttct
    48841 ggagtgtagc cttcccgatt gtgaaaccag ggtttgcagc ccttgcaatc tttaccttca
    48901 tcaatacttg gaatgactac ttcatgcaat tggtaatgtt gacttcacgt aacaatttga
    48961 ccatctcact tggggttgcg accatgcagg ctgaaatggc aaccaactat ggtttgatta
    49021 tggcaggagc tgcccttgct gctgttccaa tcgtcacagt cttccttgtc ttccaaaaat
    49081 ccttcacaca gggtattact atgggagcgg tcaaaggata atactctgcg aaaatctctt
    49141 caaactacgt cagcttcacc ttgccatact taagtattgc ctgcggttag cttcctagtt
    49201 tgttcttcaa ttttcattga gtataggaaa atcaatctat caagatacag aagtatattt
    49261 tatagattta gagaatatag aggttataag tgtctacaaa atggagggta tgcagttact
    49321 ttatgaagtt ttgtcagaca cttataaact taagaatggt tttagttaac tatcagaaac
    49381 gaaggaaaga gtatgatttt tgacgatttg aaaaacatca ccttttacaa agggattcat
    49441 cccaatttag acaaggctat cgactacctc taccaacatc gtaaagattc attcgaatta
    49501 ggaaagtatg agattgatgg ggataaagtc tttctagttg ttcaggaaaa tgtcctcaat
    49561 caagttgaga ataatcaatt tgaacaccat aagaactatg cagatttgca tttgctgata
    49621 gaagggcatg aatattcgag ctacggttca cgtatcaaag acgaggcagt agcattcgac
    49681 gaagcgactg acattggctt tgttcattgt catgaacact accccctctt gttgggttat
    49741 cacaattttg cgattttctt cccaggtgag ccacatcagc caaatggtta tgcaagcatg
    49801 gaagaaaagg ttcggaaata tctctttaaa attttgattg attaaaaata ggatgaattg
    49861 tttttttgta aagctttgac aatactctac catgaaattg atctttgtga ggtagggaaa
    49921 tgagaataaa atatttaaaa attggtatct tcttgtcagt ctgtgtatgc tacaagagct
    49981 agtttcttgg atggacaggg aattacagtt gatgagatgg cttggataat taggggcatt
    50041 gtgaatgcat tgattggtag atacataaaa ttaggtactt atgcggctaa gtatggtatt
    50101 agtatggcgc gttctatctt aaatagagca gctgcaactg cggcagaaag agtaggatta
    50161 ttgactaaga tttttggatg gattttacga atagctgtgc tcgcagctga tgtatatggt
    50221 aattttgcca acaatattgc tgtagcttgg gatgcacatg ataaaatccc taacaatggt
    50281 cgtataaact tttaaaatgc gagaatgaaa gcactttgta tttttttatt gaatatgtta
    50341 gcttggacag tgcttgcaat gataattcgt ggagggctag atggatttga taggcatact
    50401 tggagtaata ttttagctag tgcgttgtcc tcgctataca tacattatat atccatagat
    50461 agaaaaagaa aaaagtccaa aagaaaaaag aaaaaataga tttgttcatg gtagggactt
    50521 atgaaagctt tactaacaaa aaagaaaaca gtttacaaag aaaaatgatg gaggagtaaa
    50581 catggcacaa aaaggagtaa gccttatcaa ggcagcattt gatacagata actttcttat
    50641 gcgttttagt gagaaagtct tggacatcgt gacagccaat cttctttttg tcgtctcttg
    50701 tttgcccatc gtgacgattg gagtggctaa aatcagcctc tacgagacca tgtttgagat
    50761 taagaagagc agacgggtgc ctgtttttaa aatctatcta agatctttca agcaaaatct
    50821 gaaactaggt cttcagttag gtctgcttga gttgggcatt gtttcattaa gcctcctaga
    50881 tctttatctc ttttggggtc aaacagctct gccctttcaa ttgctgaaag ctatttgtct
    50941 aggcattctg attttcctta cgattgtgat gttggctagc tatccaatcg cggcacgtta
    51001 tgatttaact tggaaagaag tgttacaaaa agggcttatc ttggcaagtt ttaactttcc
    51061 ttggttcttc ctcatgttag ccattcttgt cctcattgtg atggttcttt atctgtctgc
    51121 cttcagtcta cttttaggtg gctcagtctt cctgcttttt gggtttggac tattggtttt
    51181 tatccagact ggattgatgg agaaaatttt cgcaaaatac caataggagc cttatttctg
    51241 aaactacttt caaaggctcc aaacgctatt ctataagcga gaaactaaaa tctaagtgtc
    51301 tataagatat tgaaagcctt ttcggtaaag tgtatactaa agaagtaaag aaaaatctgc
    51361 tttagcagaa aataaaaata ataggagaaa atctatgtca gatttgaaaa aatacgaagg
    51421 tgtcattcca gccttctacg catgttatga tgatcaagga gaagtaagcc cagaacgtac
    51481 gcgtgccttg gttcaatact tcattgataa aggtgttcaa ggtctttatg tcaatggttc
    51541 ttctggtgaa tgtatctacc aaagcgttga agatcgcaag ttgattttgg aagaagtcat
    51601 ggcggtagcc aaaggtaaat tgaccattat tgcccatgtt gcttgcaata atactaaaga
    51661 tagtatggaa cttgctcgcc atgctgaaag cttgggagta gatgctattg caacgattcc
    51721 accaatttat ttccgcttgc cagaatactc agttgccaaa tactggaacg atatcagttc
    51781 tgcagctcca aacacagact acgtgattta caacattcct caattggcag gggttgcttt
    51841 gactccaagc ctttacacag aaatgttgaa aaatcctcgt gttatcggtg tgaagaactc
    51901 ttctatgcca gttcaagata tccaaacctt tgtgagcctt ggtggggaag accatatcgt
    51961 ctttaacggt ccagatgaac agttcctagg tggtcgcctc atgggtgcca aagctggtat
    52021 cggtggtact tatggtgcta tgccagaact cttcttgaaa ctcaaccaat tgattgcgga
    52081 taaagattta gaaacagcgc gtgagttaca gtatgctatc aacgctatca ttggtaaatt
    52141 gactgctaca catggaaata tgtacggtgt catcaaagaa gttttgaaga tcaatgaagg
    52201 cttgaatatt ggttctgttc gttcaccatt gacaccagtg actgaagaag atcgtccagt
    52261 tgtagaagcg gctgctgcct tgattcgtga aaccaaggag agcttcctct aatctaaaag
    52321 gaggtattta tgacatacta cgttgcaatt gatatcggtg gaaccaacat caagtatggt
    52381 ttggttgatc aagaggggca acttcttgaa tcgcatgaaa tgccaactga ggcgcataag
    52441 ggtggacctc atatcttaca aaagaccaaa gatatcgtag ctagttattt agaaaaaggc
    52501 ccagtagcag gtgttgccat atcttctgct gggatggtgg atccggataa gggtgagatt
    52561 ttctatgctg ggccgcaaat ccctaactac gcaggcaccc agttcaaaaa ggaaatcgaa
    52621 gaaagcttta ctattccttg tgagattgaa aatgatgtca actgtgcagg tcttgctgag
    52681 gcagtatctg gttcaggcaa gggagcaagt gtgacacttt gcttgaccat tggaacaggt
    52741 atcggtggtt gcttgattat ggataggaaa gtcttccatg gttttagcaa ttcagcctgt
    52801 gaagtcgggt atatgcatat gcaggatgga gcttttcaag acttggcttc tacaacagct
    52861 ttagtgaaat atgtagctga agcccatgga gaagatgttg atcagtggaa tggccgtaga
    52921 attttcaaag aagccactga aggaaacaaa atctgcatgg aaggtattga ccgtatggtt
    52981 gactatctag gaaaaggtct ggcaaatatt tgctacgttg ccaatccaga agtggttatt
    53041 cttggtggtg gtatcatggg gcaagaggct atcctcaaac ctaagatccg cacagccttg
    53101 aaagaggctt tggtaccaag tttagcagaa aaaacacgat tagaatttgc ccatcaccaa
    53161 aatacagcag ggatgttggg tgcatattat cattttaaga caaaacaatc ctagtttggc
    53221 tcagccaaac taggattttc ttacacgttt ttgtctacga tagccgttga gttttttatt
    53281 ttcccagtag ctattaaaga ttttttcctt gctttcgcga ttgatttcca aaaagtaggc
    53341 ataaatcaaa tcgataaaga agagcatagg aagttgagcg gatattcgtt ggatatagga
    53401 gggttggcta tgggtggcta caagaacagt ctctgtatag gtctggctat ctttattggg
    53461 aacacttgta aagagtacag tctttgcccc catctccttg gcatctaata gactatctaa
    53521 aatagaagga gttgagcctg aaagtgagaa acccagtact agacaatttt catccatgat
    53581 gctggttgtc caggcaaagc cgtcttggtc tgtcaaagct tcgcagacca cacctagtcg
    53641 cataaaacgt aatttcattt cacgggcgac gaggccagaa ctccctgttc caaagaagta
    53701 gatacgctca gcatcttcga ttagctgggc aattcgctct agttggattt cgtcaatcaa
    53761 gtcttgtgtt tgttccctca tattgctata acttctgagg actcgtttgg tcagtggact
    53821 atgcttggag acttggttgg cttgattttc tgcctgatgt tgatattgga aaataaattc
    53881 tcggtagcca gtaaagccac actttttagc aaagcgggtc aaagcagctt gagaaatatg
    53941 taatttttgg gtgacttgtt gagaagataa atcatctgta atcgtttcag cttgcaaaaa
    54001 atagcgagcg atttcttgct ctaggtctgt catttcttca aaatgtgaat caatgatagt
    54061 tgcgatatct ggtttgtcca tagggaaagc tcctttacat gagtcatact ggaagactag
    54121 atcagggaat agtcacactt cattataaca cataatataa ggatagataa ataaaaacac
    54181 atctctgttt taaaaacgaa aaaattgaaa aagcttcttt cttttccata attttctact
    54241 caaattgtgg tacaattaag agtaagattt taagttagaa atgagactga tttgtatgag
    54301 aaaatttaac agccattcga ttccgattcg gcttaattta ttgttttcaa tcgtcatttt
    54361 actctttatg accattattg gtcgtttgtt gtatatgcag gttttgaaca aggattttta
    54421 cgaaaaaaag ctagcctcag ctagtcagac caagattaca agcagttcag cccgtgggga
    54481 aatttatgat gctagtggaa aacctttggt agaaaatacg ttaaagcagg ttgtttcctt
    54541 tacgcgtagc aataaaatga cggctacaga cttaaaagaa acagctaaaa agttactgac
    54601 ttatgtgagc atcagttctc caaatttgac agaacgccag ctggcggatt actatttggc
    54661 tgatcctgaa atctataaaa aaacagtgga agctctccca agcgagaaac gcttggattc
    54721 agatggaaat cgtctatccg aatcagaact gtataacaat gcggtcgata gtgtccaaac
    54781 aagtcaacta aactatacag aggatgaaaa gaaagaaatc tatcttttta gtcagttaaa
    54841 tgctgttgga aactttgcga caggaaccat tgctacagat cctctaaatg attctcaggt
    54901 ggctgttatt gcctctattt caaaggagat gcctggcatt agtatttcta cttcttggga
    54961 tagaaaggtt ttggaaactt ccctttcttc tatagttggg agtgtatcca gtgaaaaagc
    55021 tggtctccca gcggaagaag cagaagccta tcttaaaaaa ggctattctc taaatgatcg
    55081 tgtaggaacc tcctatttgg aaaagcaata tgaagagacc ttacaaggaa aacgctcggt
    55141 aaaagaaatc catctggata aatatggcaa tatggaaagc gtggatacaa ttgaggaagg
    55201 tagtaaggga aacaatatca aactgaccat tgatttggcc ttccaagata gcgtggatgc
    55261 tttactgaaa agttatttca attctgagct agaaaatggt ggagccaagt attctgaagg
    55321 tgtctatgca gtcgccctta acccaaaaac aggtgcggtt ttgtctatgt cagggattaa
    55381 acatgacttg aaaacgggag agttgacgcc tgattccttg ggaacggtaa ccaatgtctt
    55441 tgttccaggt tcggttgtca aggcggcgac catcagctca ggttgggaaa atggagtctt
    55501 gtcaggaaac cagaccttga cagaccagtc cattgtcttc caaggttcag ctcccatcaa
    55561 ttcttggtat actcaggctt acggttcatt ccctatcaca gcggtccaag ctctggagta
    55621 ttcatcaaat acctatatgg tccaaacagc cttaggtctt atggggcaaa cctatcaacc
    55681 caatatgttt gtcggcacca gcaatctaga gtctgctatg gagaaactgc gttcaacctt
    55741 tggcgaatat ggcttgggta ctgcgacagg aattgaccta ccagatgaat ctactggatt
    55801 tgttcccaaa gagtatagct ttgctaatta cattactaat gcctttgggc agtttgataa
    55861 ctatacgccg atgcagttgg ctcagtatgt agcaactatt gcaaataatg gtgttcgtgt
    55921 ggctcctcgt attgttgaag gcatttatgg taataatgat aagggaggac tgggtgactt
    55981 gattcagcaa ctgcaaccga cagagatgaa taaggtcaat atatccgact ccgatatgag
    56041 catcttgcac caaggttttt atcaggttgc ccatggtact agtgaattga caactggacg
    56101 tgccttttca aatggcgcct tggtatccat tagcggaaaa acaggtacag ccgaaagcta
    56161 tgtggcagat ggtcagcaag caaccaatac caatgcggtg gcctatgccc catctgataa
    56221 tccccaaatc gctgttgcag tggtctttcc tcataatacc aatctaacaa atggtgtagg
    56281 accttccatt gcgcgtgaca ttatcaatct gtatcaaaaa taccatccaa tgaactagaa
    56341 aggaaattat gctttatcca acacctattg ccaagttgat tgacagttat tctaagttac
    56401 caggtatcgg gattaagacg gctacgcgtc tggcctttta tacgattggg atgtctgctg
    56461 atgatgtcaa tgaatttgca aaaaatctcc tttctgctaa gagagaattg acatattgtt
    56521 ctatttgtgg acgtttgaca gacgacgatc cttgttctat ctgtactgat ccgactcgtg
    56581 accagacaac aattttagtt cttgaggata gtagagatgt ggcagccatg gaaaatatcc
    56641 aagaatacca tggactctat catgtcctgc atggcctcat ttctcctatg aatggtatca
    56701 gtccggacga tatcaatctc aagagcctta tgactcgtct tatggatagt gaggtttcag
    56761 aagtgattgt ggcgactaat gctacagccg atggtgaagc gacttccatg tatctttcac
    56821 gtttgctcaa gccggctggt atcaaggtta cgcgtttagc acgaggtctc gctgtgggag
    56881 cggacattga gtatgcggac gaagtgacac tcttacgagc cattgaaaat cggacagagt
    56941 tgtaagtgta ggcaaattta cgaactccat tcatttataa aaaatcaaag aggctgaaaa
    57001 tcgttcctat cggcctcttt ttgtatagtg tgatgagtag gatcaggttc aagttttaaa
    57061 aaaccaagca aatatgatat actaaagagc gagtattcta gtagaattag gacaaataat
    57121 atgaaacaaa cgattattct tttatatggt ggacggagtg cggaacgcga agtctctgtc
    57181 ctttcagctg agagtgtcat gcgtgcggtc aattacgacc gtttcacagt caagactttc
    57241 tttatcagtc agtcaggtga ctttatcaaa acgcaggaat ttagtcatgc tccggggcaa
    57301 gaagaccgtc tcatgaccaa tgaaaccatt gattgggata agaaagttgc accaagtgct
    57361 atctacgaag aaggtgcagt ggtctttcca gtccttcacg ggccaatggg agaagatggc
    57421 tctgttcaag gattcttgga agttttgaaa atgccttacg ttggttgcaa cattttatca
    57481 tcaagtcttg ccatggataa aatcacgact aagcgtgttc tggaatctgc tggtattgct
    57541 caagttcctt atgtggctat cgttgaaggc gatgatgtga ctgctaaaat cgctgaagtg
    57601 gaagaaaaat tggtttatcc agtcttcact aagccgtcaa acatggggtc tagtgtcggt
    57661 atttctaagt ctgaaaacca agaagaactc cgtcaagcct taaaacttgc cttccgatat
    57721 gacagccgtg tcttggttga gcaaggagtg aatgcccgtg aaattgaggt tggcctcttg
    57781 ggtaactacg atgtcaagag cacgctacca ggagaagttg tcaaggacgt tgccttttat
    57841 gactacgatg ccaagtatat tgataacaag attactatgg atattcctgc caaaatcagt
    57901 gatgatgtgg tggctgtcat gcgtcaaaat gcagaaacag ccttccgtgc cattggtggc
    57961 cttggtctat ctcgttgcga tttcttctat acagataagg gagagatttt tctcaacgag
    58021 ctcaatacca tgccaggttt cacccagtgg tctatgtacc cactactttg ggacaatatg
    58081 gggatcagct acccaaaact aatcgagcgt ttggttgacc ttgccaagga aagttttgac
    58141 aagcgcgaag cgcatttgat ataaaatgaa agagagggta gaagccagaa ccatcactgc
    58201 aaggtgacta gagttctcgg acttcagccc tttttaaagg agtagaaatg aaattaacaa
    58261 tccatgaaat tgcccaagtt gttggagcca aaaatgatat cagtatcttt gaggacaccc
    58321 agttagaaaa agctgagttt gatagtcgtt tgattggaac tggagattta tttgtgccac
    58381 ttaaaggtgc gcgtgatggc catgacttta ttgaaacagc ctttgaaaat ggtgcagcag
    58441 taaccttgtc tgagaaagag gtctcaaatc atccttacat tctagtagat gatgttttga
    58501 cagcctttca atccctagca tcctactatc ttgaaaaaac gactgttgat gtctttgctg
    58561 ttacaggttc aaatggcaag acaacgacta aggatatgtt ggcgcattta ctgtcaacga
    58621 cctacaagac ctacaaaaca caaggcaact acaataacga gattggcctt ccctacacag
    58681 ttctacacat gcctgaagga acagaaaagt tggttttgga gatggggcag gaccacttgg
    58741 gcgatattca tctcttgtct gaattggctc gtccaaaaac agccatcgtg accttggttg
    58801 gagaagccca tttggccttt ttcaaagacc gttcagagat tgctaaggga aaaatgcaaa
    58861 ttgcagacgg aatggcttca ggttccttgc ttttagcgcc ggctgaccct atcgtagagg
    58921 actatttgcc aactgataaa aaggtggtcc gttttgggca aggggcagag ttggaaatca
    58981 cagacttggt tgagcgtaag gacagtctga cctttaaggc taatttcttg gaacaagccc
    59041 ttgatttgcc agtgactggt aagtacaatg ctaccaatgc taagattgca tcctatgttg
    59101 ccctacaaga aggagtttca gaggagcaaa ttcatcaggc cttccaaggt cttgaattga
    59161 cgcgtaatcg tactgagtgg aagaaagcag ccaatggagc agatatcctg tcagacgtat
    59221 ataatgccaa tccaactgct atgaagctga ttttagagac tttctctgcc attccagcca
    59281 atgaaggtgg caagaaaatt gcagtgttgg cggatatgaa ggagcttggt gaccagtcta
    59341 ttcaactcca taaccagatg attttgagcc tttctccaga tgtgcttgat accgttattt
    59401 tctatggaga agacattgct gaattagccc aattggccag tcaaatgttc ccaattggcc
    59461 acgtttacta cttcaagaaa acagaagacc aagaccaatt tgaagaccta gtcaagcagg
    59521 tcaaggaaag cctcagtgcc aatgatcaaa tcttactcaa gggctctaac tctatgaacc
    59581 tagccatgtt ggtagaaagt ttagaaaatg aaaccaagtg attttgttaa gtatctgcaa
    59641 agaatgattg ctcttacaga tactggctta acctttacaa aagatccttt tgaccgtgag
    59701 cgctacgagg acttgcgaag tctgttatct gaaatgttga atcagggatc agacctggat
    59761 gcagaagaag tagcagaagt cttgaaacca acttcagctt atgcgactcc cttaatggac
    59821 gtccgtgctt ggattgttga ggatgagaag atttgtctgg ttaggggaca aggagaggat
    59881 agttgggctt tgccaggtgg ctttggtgaa gtcggctatt ctccaactga aaatattctc
    59941 aaggaaattg aagaagaaac cggttttaaa gccaaagttg aaagactgct agctgttttt
    60001 gataccaatc gtttccaact acagagcaaa caatatgcaa agtttgtttt tgaatgtaag
    60061 cttctcgatg gacaattcca agaaaatcaa gaaatcgctg accttcaatt ttttgcaatt
    60121 gaccaactgc cgaatttatc tgaaaagcgc attaccaagg agcaaatcga gattctttgg
    60181 caggtttatc aaggtcagag ggagcaatat cttgactaag aagatgatta tcgtatttct
    60241 aaattcattt ttcttaagta gcatggtata ataaaatgca ggaaaatttt gaatcatgag
    60301 gaagactaga tgaatttatg ggatattttc tttacgactc aggcaaccga gccgcccaaa
    60361 tttgaccttt tttggtatgt tagcctattt acgctcttag ccttaacctt ttatacagcc
    60421 catcgctatc gtgaaaagaa ggtttaccaa cgatttttcc aaatcttgca gactgttcag
    60481 ttaatccttc tttatggttg gtactgggtc aatcatatgc cactgtcaga aagcctaccc
    60541 ttttaccatt gccgtatggc tatgtttgtg gtactcttgc ttcctggtca gtccaaatat
    60601 aaacaatact ttgcattatt gggaacattt gggacattag cagcctttgt ttatccagtg
    60661 ccagatgctt acccttttcc acatatcacc attctatcct ttatctttgg tcatttagca
    60721 ctcttgggga actctctagt ttatctattg agacagtata atgcgcgatt gctggatgtg
    60781 aagggaattt ttctcatgac ctttgcccta aatgccttga tttttgtggt caatttggtg
    60841 acaggtggcg attacggatt tttgacaaaa ccgccattgg ttggggatca cggtctagta
    60901 gctaattatt tacttgtttc aattgtgctg gtagctacta tcagtttgac taagaaaatc
    60961 ttagaattct ttttagctca agaagcagaa aaaatgattg caaaggaagc ttaacacaga
    61021 gctttctttt ttgctcttag agagttttta caagcagctt ataaaataag aatttctgaa
    61081 tagacaaact caaaaaatgg ctgggaaatt taggaaaaaa gcaagcacga ttaaattttt
    61141 tgtgttataa tattttgtga atagctatgc ctatgtttag ctatggaata atacgaagtg
    61201 cgaaacttgg aagatagaga ggaagcgatg taatggctag agaaggcttt tttacaggtc
    61261 tagatattgg aacaagctct gtcaaggtgc ttgtggccga gcagagaaat ggtgaattaa
    61321 atgtaattgg cgtgagtaat gccaaaagta aaggtgtaaa ggatggaatt attgttgata
    61381 ttgatgcagc agcaactgct atcaagtcag ccatttccca agcggaagaa aagtcaggca
    61441 tttcgattaa atcagtaaat gtcggcttgc ctggtaatct tttgcaggta gaaccaactc
    61501 aggggatgat tccagtaaca tctgatacta aggaaattac ggatcaagat gttgaaaatg
    61561 ttgtcaaatc agctttgaca aagagtatga cacctgaccg tgaagtcatt acctttattc
    61621 ctgaagaatt tattgtggat ggtttccaag ggattcgtga cccacgtggc atgatggggg
    61681 ttcgccttga aatgcgtggt ttgctttata caggacctcg tactatcttg cacaatttgc
    61741 gtaagacggt tgagcgtgca ggtgttcagg ttgaaaatgt tatcatttca ccactagcaa
    61801 tggttcagtc tgttttgaac gaaggggaac gtgaatttgg tgctacagtg attgatatgg
    61861 gggcaggtca aacgactgtc gctacaatcc gtaatcaaga actccagttc acacatattc
    61921 tccaagaagg tggagattat gtaactaaag atatctccaa ggttttgaaa acctctcgca
    61981 aattagcgga aggcttgaaa ctgaattacg gggaagccta tccgcctctt gcaagcaaag
    62041 aaaccttcca agtagaggtt attggagaag tggaagcagt cgaagtgacg gaagcctact
    62101 tgtcagaaat tatttctgca cgaatcaagc acatccttga acaaatcaag caagaattag
    62161 atagaaggcg tctattggac ctccctggtg gtattgtctt aatcggtggg aatgccattt
    62221 taccaggtat ggttgagctt gctcaggaag tctttggcgt ccgtgtcaag ctttatgttc
    62281 caaatcaagt tggtatccgt aatccagcct ttgcgcatgt gattagttta tcagaatttg
    62341 cgggtcaatt aacagaagtt aatcttttgg ctcagggagc gataaaaggt gagaatgact
    62401 taagtcatca gccaattagt tttggtggga tgctgcaaaa aacagctcag tttgtacaat
    62461 caacgcctgt tcaaccagct cctgctccag aagtagagcc ggtggcgcct acagaaccaa
    62521 tggcggattt ccaacaagct tcacaaaata aaccgaaatt agcagatcgt ttccgtggct
    62581 tgatcggaag catgtttgac gaataaagag gaaaaataaa ttatgacatt ttcatttgat
    62641 acagctgctg ctcaaggggc agtgattaaa gtaattggtg tcggtggagg tggtggcaat
    62701 gccatcaacc gtatggtcga cgaaggtgtt acaggcgtag aatttatcgc agcaaacaca
    62761 gatgtacaag cattgagtag tacaaaagct gagactgtta ttcagttggg acctaaattg
    62821 actcgtggtt tgggtgcagg aggtcaacct gaggttggtc gtaaagccgc tgaagaaagc
    62881 gaagaaacac tgacggaagc tattagtggt gccgatatgg tcttcatcac tgctggtatg
    62941 ggaggaggct ctggaactgg agctgctcct gttattgctc gtatcgccaa agatttaggt
    63001 gcgcttacag ttggtgttgt aacacgtccc tttggttttg aaggaagtaa gcgtggacaa
    63061 tttgctgtag aaggaatcaa tcaacttcgt gagcatgtag acactctatt gattatctca
    63121 aacaacaatt tgcttgaaat tgttgataag aaaacaccgc ttttggaggc tcttagcgaa
    63181 gcggataacg ttcttcgtca aggtgttcaa gggattaccg atttgattac caatccagga
    63241 ttgattaacc ttgactttgc cgatgtgaaa acggtaatgg caaacaaagg gaatgctctt
    63301 atgggtattg gtatcggtag tggagaagaa cgtgtggtag aagcggcacg taaggcaatc
    63361 tattcaccac ttcttgaaac aactattgac ggtgctgagg atgttatcgt caacgttact
    63421 ggtggtcttg acttaacctt gattgaggca gaagaggctt cacaaattgt gaaccaggca
    63481 gcaggtcaag gagtgaacat ctggctcggt acttcaattg atgaaagtat gcgtgatgaa
    63541 attcgtgtaa cagttgttgc aacgggtgtt cgtcaagacc gcgtagaaaa ggttgtggct
    63601 ccacaagcta gatctgctac taactaccgt gagacagtga aaccagctca ttcacatggc
    63661 tttgatcgtc attttgatat ggcagaaaca gttgaattgc caaaacaaaa tccacgtcgt
    63721 ttggaaccaa ctcaggcatc tgcttttggt gattgggatc ttcgccgtga atcgattgtt
    63781 cgtacaacag attcagtcgt ttctccagtc gagcgctttg aagccccaat ttcacaagat
    63841 gaagatgaat tggatacacc tccatttttc aaaaatcgtt aagtaaatga atgtaaaaga
    63901 aaatacagaa cttgtttttc gagaagttgc agaggctagt ctgagtgctc atcgagagag
    63961 tggttcggtc tctgtcattg cagttaccaa gtatgtagat gtaccgacag cggaagcctt
    64021 gcttccgcta ggtgttcatc atatcggtga aaatcgtgta gataagtttc tggaaaaata
    64081 tgaagcttta aaagatcgag atgtgacttg gcatttgatt ggtaccttgc aaagacgtaa
    64141 ggtgaaagat gtcattcaat acgttgatta tttccatgca ttggactcag taaagctagc
    64201 aggggaaatt caaaaaagaa gtgaccgagt catcaagtgt ttccttcaag taaatatttc
    64261 taaagaagaa agcaaacacg gtttttcgag agaggaactg ctggaaatct tgccagagtt
    64321 agccagacta gataagattg aatatgttgg tttaatgacg atggcacctt ttgaggctag
    64381 cagtgagcag ttgaaagaga ttttcaaggc ggcccaagat ttacaaagag aaattcaaga
    64441 gaaacaaatt ccaaatatgc ctatgaccga gttaagtatg ggaatgagtc gtgattataa
    64501 agaagcgatt caattcggtt ccacttttgt tcgtataggt acatcatttt ttaagtagga
    64561 gagaaccatg tctttaaaag atagattcga tagatttata gattatttta cggaggatga
    64621 ggattcaagt ctcccttatg aaaaaagaga tgagcctgtg tttactccag taaattcttc
    64681 acaggaaccg gctctcccaa tgaatcaacc ttcacagtcg gctggtacaa aagagaacaa
    64741 tatcaccaga cttcatgcaa gacaacagga attggcaaat cagagtcagc gtgcaacgga
    64801 taaggtcatt atagatgttc gttatcctag aaaatatgag gatgcaacag aaattgttga
    64861 tttattggca ggaaacgaaa gtatcttgat tgattttcag tatatgacag aggtgcaggc
    64921 tcgtcgttgt ttggactatt tggatggagc ttgtcatgtt ttagctggaa atttgaaaaa
    64981 ggtagcttct accatgtatt tgttgacacc agtgaacgtt attgtaaatg ttgaagatat
    65041 ccgtttacca gatgaagatc aacagggtga gttcggtttt gatatgaagc gaaatagagt
    65101 acgataatga tttttttaat tcgtatgatt tataatgcag tggatattta ctccctaatt
    65161 ttggtagcct tcgctgtcat gtcttggttt ccaggtgcct acgaatccag tttaggtcgt
    65221 tggattgtag cgttggtaaa accagtgctt gctcccttgc aacgcctgcc tttacagata
    65281 gcgggtcttg atttatctgt ttgggttgcg attgttttgg ttcgattttt aggagaaaac
    65341 ctagtgcgtt ttctggcgat gataggatga ataaagggat ttatcagcat ttctccatag
    65401 aagatcgtcc atttcttgac aagggaatgg aatggataaa gaaggtagaa gatagctatg
    65461 ctcctttttt aactcctttt atcaatcctc atcaggagaa gctattaaag attttggcca
    65521 aaacctatgg tcttgcttgt agcagtagtg gggaattcgt ctcgagtgag tatgttcgag
    65581 ttttattata cccagattat ttccaaccag agttttcaga ttttgaaata tctctccagg
    65641 aaattgtgta ttccaataaa tttgaacatt taacgcatgc taagatttta gggacagtca
    65701 tcaatcaatt agggattgaa cggaaacttt ttggagatat cctagtagat gaagaacggg
    65761 cgcagattat gattaatcag cagtttcttc ttctctttca agatggacta aagaaaattg
    65821 gtcgtatacc tgtttcgctg gaggaacgtc ctttcaccga gaaaatagat aagctagaac
    65881 agtatcgaga actggattta tctgtgtcta gttttcgatt agatgttctt ttatcaaatg
    65941 ttttgaaact atctaggaat caagcaaacc agttgattga aaagaaactt gtccaagtaa
    66001 attatcatgt ggtagacaaa tcagattaca ctgttcaagt tggcgacttg attagtgtga
    66061 gaaaatttgg tcgcttgaga ttacttcaag ataagggaca aacgaaaaaa gagaagaaaa
    66121 aaataaccgt ccagttatta ttaagtaagt gaggaataga atgccaatta catcattaga
    66181 aataaaggac aagacttttg gaactcgatt cagaggtttt gatccagaag aagtcgatga
    66241 atttttagat attgtggttc gtgattacga agatcttgtg cgtgcgaatc atgataaaaa
    66301 tttgcgtatt aagagtttag aagagcgttt gtcttacttt gatgaaataa aagattcatt
    66361 gagccagtct gtattgattg ctcaggatac agctgagaga gtgaaacagg cggcgcatga
    66421 acgttcaaac aatatcattc atcaagcaga gcaagatgcg caacgcttgt tggaagaagc
    66481 taaatataag gcaaacgaga ttcttcgtca agcaactgat aatgctaaga aagtcgctgt
    66541 tgaaacagaa gaattgaaga acaagagccg tgtcttccac caacgtctca aatctacaat
    66601 tgagagtcag ttggctattg ttgaatcttc agattgggaa gatattctcc gtccaacagc
    66661 tacttatctt caaaccagtg atgaagcctt taaagaagtg gttagcgaag tacttggaga
    66721 accgattcca gctccaattg aagaagaacc aattgatatg acacgtcagt tctctcaagc
    66781 agaaatggca gaattacaag ctcgtattga ggtagccgat aaagaattgg ttgaatttga
    66841 agctcagatt aaacaggaag tggaagctcc aactcctgta gtgagtcctc aagttgaata
    66901 agagcctctg ctcatccagt tggcccaatg tatgaagaac cagaagtagc tccagtgcat
    66961 ccgacaggtc caacaccagc tacagaaact gttgattcaa taccgggatt tgaagcaccg
    67021 caagaatttg ttacaatttt ataagaaata ttctgagaac aatatcttat ccttatattc
    67081 ccagcgagca ggagatggtg tgagtcctgc attccctatc gataagatta tcctctcaaa
    67141 ctatcaagtc tgaatctagt aagatttgac gttccccacg ttacgggata agagggagaa
    67201 agactaaaac tttttccgaa taaaggtggt accacgattt tcgtcctttt tggaagtcgt
    67261 ggtttttaat ttgttattat ttataaagga gataccatga aactcaaaga tacccttaat
    67321 cttgggaaaa cagctttccc aatgcgcgca ggacttccta ccaaagagcc agtttggcaa
    67381 aaggaatggg aagatgcaaa actttatcaa cgtcgtcaag aactaaatga agggaaaccg
    67441 cattttgtct tgcatgatgg ccctccatac gcaaacggaa atatccacgt tggacatgcc
    67501 atgaatcata tctcaaaaga tatcatcatt cgttctaagt ctatgtcagg ttttaactca
    67561 ccttatatcc cgggttggga tactcatggt ctgccaatcg agcaagtttt ggcaaagcaa
    67621 ggtgttaagc gcaaagaaat ggacttggtt gagtacttga aactttgtcg tgagtacgct
    67681 ctttctcaag tttataaaca acgtgatgat ttcaagcgct tggggatgtc aggtgactgg
    67741 gaaaatttat atgtgacttt aactccagac tacgaagcag cccaaatccg tgtatttggt
    67801 gagatggcta ataaaggcta tatctaccgt ggtgcaaaac cagtttactg gtcttggtca
    67861 tctgagtcag cccttgctga agcggaaatt gaataccatg atttggtttc aacttccctt
    67921 tactatgcca ataaggtaaa agatggcaaa ggtgttctag atacagatac ttatatcgtt
    67981 gtttggacaa ctactccatt tactattaca gcttctcgtg gtttgactgt tggtgcagat
    68041 attgattacg ttttggttca acctgctggt gaaactcgta agtttgtggt tgctgcagaa
    68101 ttgttgatta gcttgtctga gaaatttggt tgggctgatg ttcaagtgtt ggcaacttac
    68161 cgtggtcaag aactcaacca catcgtaact gaacacccat gggatacagc tgtagatgag
    68221 ttggttatcc ttggtgacca cgttacaact gactctggta ctggtattgt ccatacagcc
    68281 cctggttttg gtgaggatga ctacaatgtt ggtattgcta atggtcttga agtcgcagtg
    68341 actgttgacg aacgtggtat catgatgaag aatgctggtc ctgagtttga aggtcaattc
    68401 tatgaaaagg tagttccaac tgttattgaa aaacttggta acctccttct tgctcaagaa
    68461 gaaatctctc actcataccc atttgactgg cgtaccaaga aaccaattat ttggcgtgca
    68521 gtaccacagt ggttcgcctc tgtttcaaaa ttccgccaag aaatcttgga tgctattgat
    68581 aaagttaaat tccatacaga atggggtaaa gtacgtctat ataatatgat tcgtgaccgt
    68641 ggtgactggg ttatctctcg tcaacgtgct tggggtgttc cacttccaat cttctatgca
    68701 gaagacggta cagctatcat gacagctgaa acgattgaac acgtagctca actctttgaa
    68761 gtacatggtt caagcatttg gtggaaacgt gataccaaag acctcttgcc agaaggattt
    68821 acacatccag gttctccaaa tggggagttc aagaaagaaa cagatattat ggatgtatgg
    68881 tttgactcag gctcatcatg gaatggtgtt ttggtaaatc gtccaaatct gacttatcca
    68941 gctgaccttt accttgaagg ttctgaccaa taccgtggtt ggttcaactc atcactcatt
    69001 acatctgttg ccaaccatgg cgtagcacct tacaaacaaa tcttgtcaca aggttttgcc
    69061 cttgatggta aaggtgagaa gatgtctaaa tctcttggaa ataccattgc tccaagtgat
    69121 gttgaaaaac aatttggtgc tgaaatcttg cgtctttggg taacaagtgt tgactcaagc
    69181 aatgacgtgc gtatctctat ggatatcttg agccaagtct ctgaaactta ccgtaaaatc
    69241 cgtaacaccc ttcgtttctt gattgccaat acatctgact ttaacccagc tcaagacgca
    69301 gtagcatacg aagatcttcg ctcagttgat aagtacatga ctattcgctt taaccagctt
    69361 gtcaagacca ttcgtgatgc ttacgcaaac tttgaattct tgacaagcta caaggccttg
    69421 gtgaacttta tcaacgttga cttgtcagcc ttctaccttg attttgccaa agacgttgtt
    69481 tacatcgaag gtgctaaatc attggaacgc cgtcaaatgc agactgtctt ctatgacatt
    69541 cttgtcaaaa tcacgaaact cttgactcca atccttcctc acactgcgga agaaatctgg
    69601 tcatatcttg agtttgaagc cgaagacttc gttcaattgt cagaattgcc agaagcggaa
    69661 acttttgcta atcaagaaga aatcttggat acatgggcag ccttcatgga cttccgtgga
    69721 caagctcaaa aagccttgga agaagctcgt aatgcaaaag taatcggtaa atcacttgaa
    69781 gcacacttga cagtttatcc aaacgaagtt gtgaaaactc tactcgaagc agtaaacagc
    69841 aatgtggctc aacttttgat cgtgtcagac ttgaccatcg cagaaggacc agctccagaa
    69901 gctgccgtta gcttcgaaga tgtagccttc acagttgaac gcgctgcagg tgaagtatgt
    69961 gaccgttgcc gtcgtattga cccaacaaca gcagaacgca gctaccaggc agttatctgt
    70021 gaccactgtg caagtatcgt agaagaaaac tttgcagatg cagtcgcaga aggatttgaa
    70081 gagaaataag attgaaaagt ctaggtaaaa ctcaatttga gaagaaaaga caactaattt
    70141 tatagtctat taaacgcatt gtatcacgtt tttgaacacc tgatatgatg cgtttttatt
    70201 tattttgaaa atttgtgagg tgtgactttt tatactcaac aagaatcaaa gagaaactta
    70261 gcaagctaac agtagtaaga taaaatagga atttgatatt agggataaga ttggtaaata
    70321 gtgtaatatt tttacaacaa taaatttata tagttatttc tggtttctga aaagtattat
    70381 attatatttt atttcatatt atacaaattt ttattttata atatcagaaa atactttttt
    70441 taaaaagcaa atatgataca attttatttg aaaaaaaata aaaaaggaga ttttattatg
    70501 aaatcaagtg gtatcgtcgg tcttgtggga ggaattttac ttcttatttg tccttttgtc
    70561 ttgttgggaa cagcggtaaa tacagctgct acaactctta atggaggagc tactgcaggg
    70621 gctttttcag gtgttgcctt actcttgaat gccttgaaga ttgcaaatct tgttcttggt
    70681 atcattgcta ttgtttacta taaaggagat aagcgtgtag gtgcagctcc gtctgtacta
    70741 atgattgttt ctggtgaagt tagtctcatt ccattcttag gatgggttgg ggggattctt
    70801 gctattatcg gaggatctct attccttgca acattgaaga aattcaaatc agaagaataa
    70861 aaggtatttt agcatgaaaa gaacaaaaaa gtttatcggt ataggagtag ctctattatc
    70921 tctttctctt ctagttgcat gtggaacata aagttcaaag aatacttcaa caagtaatga
    70981 tgagaagaca gtagcaacat ccaatagttc aaaagaaaca atcactttcg atacaccggt
    71041 tgtaacagac gatgcgattg aatcaatacg cacttatgca gattatatag atctttataa
    71101 aaatattttt gatgattatt ttactaaaac tgaggaaggt ttcaaaggca cagctatgga
    71161 aaataatgac tcgtttacta aactaaaaga gtcaactcaa aaattattcg atgcgcagaa
    71221 aaaaaggtta aataatgaag atagaataga aacaaccaaa aacaatgtga ttgccaaaca
    71281 ttgtcaaaca gtcctttcct ttttggtttt gactagcttt tttgtgaaaa attgtgtaaa
    71341 atagaataga taaacgaggg gaaacctcgg aaaatttaaa ggagaatcca tctaatggta
    71401 aaattggttt ttgctcgcca cggtgagtct gaatggaaca aagctaacct tttcactggt
    71461 tgggctgatg ttgatttgtc tgaaaaaggt acacaacaag cgattgacgc tggtaaattg
    71521 atcaaagaag ctggtatcaa atttgaccaa gcttacactt cagtattgaa acgtgctatc
    71581 aaaacaacta acttggctct tgaagcttct gaccaattgt gggttccagt tgaaaaatca
    71641 tggcgcttga acgaacgtca ctacggtggt ttgactggta aaaataaagc tgaagctgct
    71701 gaacaatttg gtgatgagca agttcacatc tggcgtcgtt catacgatgt attgcctcca
    71761 aatatggacc gtgatgatga gcactcagct cacacagacc gtcgttatgc ttcacttgac
    71821 gactcagtaa tcccagatgc tgaaaacttg aaagtgactt tggaacgtgc tcttccattc
    71881 tgggaagata aaatcgctcc agctcttaaa gatggtaaaa acgtattcgt aggagctcac
    71941 ggtaactcaa tccgtgccct tgtaaaacac atcaaaggtt tgtcagatga cgagatcatg
    72001 gacgtggaaa tccctaactt cccaccattg gtattcgaat tcgacgaaaa attgaacgtc
    72061 gtttctgaat actaccttgg aaaataaaaa attgtaagtc tagaattgat ttctaggctt
    72121 tttatgttag tatggaagta tgataaggaa taaaaaacaa gattatgtac tggcctacaa
    72181 gcaaccagct tcaaccactt acatgggttg ggaagaagaa gctttaccga taggcaatgg
    72241 ttctttagga gcaaaagtat ttggccttat aggggctgaa cggattcaat ttaatgaaaa
    72301 aagtctctgg tctggaggtc cacttcctga tagttcagat tatcagggtg gaaatcttca
    72361 ggatcagtat gtttttttag ctgagattcg gcaggctttg gagaagagag attacaatct
    72421 ggctaaggaa ctggctgagc agcacctaat tgggccaaaa acgagtcaat atgggaccta
    72481 tctgtctttt ggggatattc acattgagtt cagccagcaa ggtacgactt tgtctcaggt
    72541 gacggactat cagagacagc tgaatattag taaggcactt gtgacgactt cttatgtcta
    72601 taagggaacg cgatttgaac gtgaagcttt tgcgagtttt ccagatgatc tcttggttca
    72661 gcgttttact aaggaagggg cggaaactct agattttact ataaaactat ccttgacctg
    72721 tgatttggct tctgatgaaa agtatgagca ggaaaaatct gattacaagg agtgtaagtt
    72781 ggatattact gattctcata tcttgatgaa gggaagagtt aaggataatg atctgcggtt
    72841 tgctagttat ctagcttggg aaacggatgg agatattaga gtttggtcag atagggttca
    72901 gatatcagga gccagttatg ccaatctctt cttggccgct aagacggatt ttgcccaaaa
    72961 tcctgctagc aattatcgca agaaactaga tttagagcaa caggtgatag acttggtgga
    73021 cacagctaaa gaaaagggct atacccaatt gaaatcaagg catatcgagg actaccaagc
    73081 cttattccag cgtgttcaat tggatttgga agctgatgtt gacgcatcca ctacagatga
    73141 tttgttaaaa aattataagc cacaagaagg gcaggctttg gaggagctgt tcttccagta
    73201 tggacggtat ttattgatta gttcgtccag agactgccca gatgctctac cagctaacct
    73261 acagggagtc tggaatgcgg tcgacaatcc tccttggaat tcggactatc acttaaatgt
    73321 caatctgcag atgaattatt ggccagccta tgttaccaat ctcctagagg cggtctttcc
    73381 ggtcatcaac tatgtagatg atttgcgtgt ctatggtcgt ctagcggctg taaagtatgc
    73441 aggaatcgtc tctcagaaag gtgaggaaaa tggttggttg gttcatactc aagcgactcc
    73501 ctttggttgg acggcacctg gttgggatta ctattggggt tggtcaccag ctgccaatgc
    73561 gtggatgatg caaaccgttt atgaagccta ttcattttat agggaccaag actatcttag
    73621 ggagaaaatt tatcccatgt tgagggaaac ggttcgtttt tggaatgcct ttttacataa
    73681 ggatcagcag gcgcagcgtt gggtgtcttc tccgtcttat tctccagaac atgggccgat
    73741 ttcgattggc aatacctatg accaatctct gatttggcag ttatttcatg attttattca
    73801 ggctgctcag gaattgggac tggatgagga cttgttgact gaggttaagg agaagtctga
    73861 tttactaaat cctttgcaaa tcactcaatc tggtcgaatc agggagtggt atgaggagga
    73921 agagcagtat tttcaaaatg agaaagtgga ggcccagcat cggcacgctt cccatctagt
    73981 gggactctat cctggcaatc tctttagcta caagggacaa gagtatattg aagcggcacg
    74041 tgctagcctc aatgatcgtg gagatggcgg cacaggctgg tccaaggcta ataagatcaa
    74101 tctctgggcg cgtttgggag atggcaatcg agcccataaa ttattggcag agcagttaaa
    74161 aacatccacc ttgccaaatc tttggtgtag ccatcctcct tttcagatag atggtaattt
    74221 tggtgctact agtggcatgg cagaaatgtt actccagtct catgcagctt atctggtacc
    74281 tctagctgcc ctacctgatg cttggtcaac aggttctgtt tcaggcttaa tggcacgtgg
    74341 atattttgaa gtgagcatga gctgggaaga taaaaaactc ttacagttga ccattttatc
    74401 aaggagtgga ggagatttgc gagtttctta tccagatatt gagaagagtg tgattaaaat
    74461 gaatcaagaa aaaataaaag cgaaatgcat ggggaaagat tgtatttcgg tggcaacagc
    74521 agaaggtgat cttgttcaat tttattttta agaagatgtt ataaggcagt aatttgaaac
    74581 ttccttttaa taaggattta agaatatagg cagtttccaa ctagttgaaa aagcgttata
    74641 atgataatag gaagtaatac tcaatgaaaa tcaaagagca caaactagga agctagccgc
    74701 aggttgctca aaacagtgtt ttgaggttgt agatggaagc tgacgtggtt tgaagagaga
    74761 ttttcgagga gtataatttg tttgatagag ggtgggtctg atggcttata ttgagatgaa
    74821 acactgttac aagcgttatc aggttgggga cacggagatt gtggccaatc gtgatgtgaa
    74881 ttttgagatt gaaaaggggg agctggttat tatccttggt gcttcaggtg caggcaagtc
    74941 aacagttctt aaccttcttg ggggaatgga taccaatgat gaaggggaaa tctggattga
    75001 tggtgttaat attgcggatt atagttccca ccagcgcacc aattaccgta gaaatgatgt
    75061 ggggtttgtt tttcagtttt ataatctagt ttctaatctg acagctaagg aaaatgtgga
    75121 actggcttct gaaattgtga cagatgcctt gaatcctgat caggtcttga cagatgtagg
    75181 tctggctcat cgtctcaata actttccagc ccagctttct ggaggggagc aacagcgagt
    75241 ctccattgca cgcgcggtag ccaaaaatcc taaaattctc ctttgtgatg aaccgactgg
    75301 agccttggat tatcagacgg gcaagcaggt tttgaaaatt ctccaagaca tgtctcgtca
    75361 aaagggagcg acggtgatca tcgtgactca taatggagct ttggcgccca ttgctgatcg
    75421 cgtgattcat atgcacgatg ccagtgtcaa ggatgtggtg ctcaaccagc atcctcagga
    75481 tattgacagt ttggagtact agcatgatca agcgaaaaac ttattggaag gacttaattc
    75541 agtccttcac aggctccaag gagcgttttt tatccatctt gatcctgatg atgttgggat
    75601 ctctagcctt agtaggcctc aaagtaacca gtcccaacat ggaggcgaca gctaatgctt
    75661 atttaacaac tgctcaaacc ttggatttgg cagtcatgtc taactatggc ttggatcaag
    75721 cagaccaaga agaactaaaa cagacggagg gcgcagaggt cgagtttggc tatttgacag
    75781 atgtgactat ggataatggg caggatgcca ttcggctgta ctccaaacca gagcgaattt
    75841 caacctttca gctaagaaag ggacgacttc ctcagtcaga caaggaaatc gctttggcca
    75901 ctcatttgca aggccaatac agcgtgggac aggagattag ttttaaagaa aaagaagagg
    75961 gtcattcctc tttaaaagac catacttata ccattactgg ttttgtggat tcggctgaaa
    76021 tcctctccca gcgagatatg ggctacgcag gaagtggaag tgggactctg acagcctatg
    76081 gggtgatttt acctagtcag tttgatcaga aagtctacaa tatagctcgt ttgaaatatc
    76141 aagatttagc aggtttaaat gccttttcat cagcttatga agaaaaatcc aagcaacatc
    76201 aggaagacct tgaacaaact ttatcagata atggcaaggt acgtctgcaa cttttgaaaa
    76261 aagaaggaca agagtctcta gacaaggggc aagagaccct tgacaaggct cagactaatt
    76321 tgcaggaagg caagcgtcgt ttagcagctg ctcaagctcg tatacaggct caagaaagtc
    76381 aactagcctt gtttcctcaa gttcagagag agcaggctag tgctcaactt acccaagcca
    76441 agcaggaatt gggcaaggaa gaggataaac taaagcaagc tgaacaaaat ctagcccaag
    76501 aaaaggaaaa attagaaaaa catcagcaag tcttggacga tttggcggag ccaaggtatc
    76561 aggtctataa tcgtcagacc atgccaggtg gtcagggcta tcttatgtat agcaatgctt
    76621 catccagtat tcgagcagtg ggcaatatct ttcctgtggt actttatgcc gtagcagcca
    76681 tggtgacctt tacgaccatg actcgctttg tagacgaaga gcgaactcat gcagggattt
    76741 ttaaggcctt gggttaccgt agtaaggata ttatcgccaa gtttctcctt tatggactag
    76801 tagctgggac tgtcggaacg gctctaggta gtatacttgg tcattatttg ctagccagtg
    76861 taatttcaag tgtcattaca aaaggcatag tggtaggaga aacccagatt cagttctatt
    76921 ggacctatag cttactagct cttgtcttga gctggttggc gagtgtgtta ccagcctatc
    76981 tggtggcttg gagggaactt catgacgaag cagcccagat tctacttcct aaacctcctg
    77041 tcaaaggagc taaaatctta ttggagcgta tcggttttat ctggcgtcgt ctcagtttta
    77101 ctcataaggt aacagcccgc aacatctttc gttataagca gagaatgttg atgacaatct
    77161 ttggtgtggc aggttctgta gctctgctct ttgcaggttt gggaatccaa tcttctgtag
    77221 caggagttcc gtctaaacag tttcaacaaa tccaacagta tcagatgctt gtctctgaaa
    77281 atcctagtgc gaccaatcag gacaaggtag agctagcaga agtgttgaaa gggcaggaga
    77341 tactagccta ccagaaaatc tattctaaaa cgctagacaa ggatttcaaa ggcaaggatg
    77401 gtcttcaaaa cattactctt atgatgatag agaaggaaga tttgacttcc tttatccatc
    77461 ttcaacatca tcagcaggag ctgacattaa aagatggcat cgttattaca gctaaactcg
    77521 cccagctggc aggtgtcaag gttgggcaga ctttagaaat tgaaggtaag gaactaaagg
    77581 ttgctgctat tactgagaac tacgttggtc actttattta tatgagtcag gctagctatg
    77641 agcaacttta cggacagcta ccccaagcca acacttatct ggtttcatta agggatacca
    77701 gtgcaactag tatcgaaagt caggcgggct tgcttatgag tcaatctgcg gtgtccagcg
    77761 ttgtccaaaa tgcttcagcc attcgactct tcgactctgt cgctagctca ctcaatcaga
    77821 ccatgaccat cttggtcatc gtatcggttc tattagctat tgtcatcctt tacaatctga
    77881 ccaatatcaa cgtagctgag agaatccgtg aactctccac taccaaggtt cttggttttc
    77941 ataataatga agtcaccctc tacatttacc gtgagacgat tgtgctgtcc cttgtgggaa
    78001 tcgtacttgg tctggtagct ggtttctatt tacaccaatt tttgattcaa atgatttcgc
    78061 ctgcgactat tctcttttat ccgcaggtag gctgggaagt ctatgtaatc ccagtggcaa
    78121 cagtaagcat cattttgacc ttgcttggtt tcttcgtcaa ttattatctg agaaaggttg
    78181 atatgttaga agccctgaaa tctgtagagt aaggtagtta tttttagctg attgaacttc
    78241 tatttactaa tattcaaaaa tcctctgtca caaagagcag ggagttcttt gtgacagagg
    78301 attttttcta tagggcttta gcagctgcaa ttgcggcttc gaagtttggc tcagaattga
    78361 tattatccac gtattcaacg tagcgaatcg tattgtcagt atcgaggaca aagactgcgc
    78421 gtgctaatag gtgccattcg ttgatcaaga gggcataatc gcgcccgaaa gaatggtcaa
    78481 agtagtctga aagcataatg gcattgtcaa ggccttcagc accgcaccaa cgtttttgag
    78541 caaaaggtag gtccattgaa acagtcaata cgaccgtgtt gtccagtcca gccaattctt
    78601 cattaaaacg acgtgtttga gttgagcaga tacctgtatc gatagaagga acgacactca
    78661 agactttttt cttgccatca aaatcagcca gagatttttt agaaagatct gttgtagtaa
    78721 gagaaaaatc aagcgccttg tcgccgactt gtagttgttt acctgtaaag ctcacaggat
    78781 ttccgagaaa agttaccata ggatactcca atcttttttc ttccattgta tctaaaacag
    78841 tcagaatttt ccaataattt gaccggaaat gtgtgcatag aaaaaacgcc agctcacatg
    78901 agaatgacgt ttttcagagg cttattttgc caatccttca gcaatcttgt caaggttgta
    78961 tttcatcatg ctgtagtagc tgtcgccttc tttaccttgt tctgcgatag agtcagtaaa
    79021 gatttgtgcg tagattggga tgtttgtgtc ttgagaaaca gttttcattg gacggtcatc
    79081 cacacttgat tctacaaaga gtgatggaac ttttgtttgg cgaagttttt caaccaaggt
    79141 cttgatttgt tcaggagttc cttcttcttc agtattgatt tcccagatgt aggcacttgg
    79201 gacaccatag gctttagaga agtatttgaa tgctccttcg ctggttacaa tgagtttctt
    79261 ttcagcaggg atcttattaa atttatcctt actttcttta tcaagtttgt ctaacttatc
    79321 agtatattct ttgagatttt tttcatagaa ttctttattg ttagggtctt tggcgctcaa
    79381 ttgtttggcg atatttttag caaaaataat accgttttca aggttaagcc aagcgtgtgg
    79441 gtcttctttt cctttttcat tttgaccttc aaggtagata acatcaacgc cgtcgctgac
    79501 tgcgaagtag tctttgtttt cagttttctt ggcattttct accaattttg taaaccaagc
    79561 attgccacct gtttcaaggt tgataccgtt atagaaaatc aaatcagcct cagaagtttt
    79621 cttaacgtct tcaggaagtg gttcgtattc gtgtgggtct tgcccaatcg gaacgatact
    79681 atgaaggtca attttgtcac cagcaatatt tttagtaata tcagcgatga ttgagtttgt
    79741 agcaacaact tttagttttt gaccagaagt tgtatctttt tttccgctag cacatgctac
    79801 aagaatgatt gcagaaagaa agagaacgag taatgtacct aattttttca ttagatcctc
    79861 caatttatta gggctttgcc ccttatttta acaaatgttt atttttcagt ttcaaatatc
    79921 gttgtttggg agcgataaag aagctaatga gaaagaaact agcagctgta agcacgatac
    79981 tagaacctgc cgcaacatta aaactatagc caataaagag tcccaaaact gaagcagtag
    80041 ctccgaaggt tgaggaaagg aaaatcatac ttttcagact attagcatac agataagcag
    80101 ttgcagctgg ggtaatcagc atggctacaa tcaggatagt tccgacactt tgcatggctg
    80161 tcacagacac gagagtcagg agtaccatga gaaggtagtg atagaaattg acaggcattc
    80221 ccatggcttt agccaagagt tcatcaaagg aagttatcaa gagttgcttg aagaaaatcc
    80281 agattaacaa gaggatggct gcccccacac ccatagtaat aaacatatcc gtatcttgga
    80341 cggccaggat attaccaaaa aggatatgga aaaggtcagt tgaactttta gcgacaccaa
    80401 tcaagatgat accgagggct aagaaagaag aaaaggtaat gccgatggcg gtatcgcttt
    80461 tgataatcga gtttcctttg atgtaggtaa tgatgatggc agctagcaat ccaaagacaa
    80521 tggctccgat aaagaagtca aggcccaaga tgaaggagag ggctacacct ggtaagacag
    80581 catgtgaaat ggcatctccc atgagtgaca tcccgcgtag aataatgaaa catcccacag
    80641 ctccagctac aatcccgacg acaatagctg ttatcaaggc attttgtagg aaatggaatt
    80701 tttgcaatcc atcgataaat tctgcaatca taggtcacct ccattgaaaa agagttgatt
    80761 accgtaagct tcttttagat tggtttcggt aaaagtttct tttgttggac caaaggcaat
    80821 cacttctcga ttgacaagta agacttgatc gaagtagtgg ggaatcttgc tgaggtcgtg
    80881 gtgaacgatg agaaccgtct tcccagcttt tttcaaatct ctcagcgtat tcatgatgat
    80941 ttcctcactg acagagtcaa tcccagcaaa gggttcatcc aagaggatat agtcggcttc
    81001 ctgcaccaaa catctggcaa tcaagacccg ctggaattga cctccagaca gttgactaat
    81061 ttgacgttca gcgtagtcag ctaggccgac gatttcaagg gcctcttgca ctttcttcca
    81121 atgtttagcc tttaaacttc gaaagagagg aatagaggga aatagtccta acgagacgca
    81181 ttccttgacc ttgatgggaa agttgtagtc gatattgatt ttttgttcga cataggcaat
    81241 tcggtgtaag gattttttaa cttccttgtc atcgagaaat gcctgacctt gatgtgggat
    81301 aattcccagc atacctttta atagtgttga tttcccagcg ccgtttggac caatgatgcc
    81361 ggtaattgtt ggtccatgga gcactagtga aatatcctta agtgccaacg tttctttgta
    81421 ggagacactg aggttttcga tacgtatcat aaacttgtat tcctcctgtc tcttaatata
    81481 cattaaaaaa aaaattaagt caagttaatt tttgaaaaaa ttaaaataat aactga
//
LOCUS       DAVDWM010000006        68901 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVDWM010000006 DAVDWM010000000
VERSION     DAVDWM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.006"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            301..435
                     /locus_tag="VUW49_000490"
                     /pseudo
     CDS             301..435
                     /locus_tag="VUW49_000490"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(673..2105)
                     /locus_tag="VUW49_000491"
                     /pseudo
     CDS             complement(673..2105)
                     /locus_tag="VUW49_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2361..4211)
                     /locus_tag="VUW49_000492"
     CDS             complement(2361..4211)
                     /locus_tag="VUW49_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HET7980459.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFAKQEIASRLEFLLNC
                     VNSLEGLLTKSKELNLTIDLKQKNVIFILEENGKQFSLSHKKISDEKLYDVNFFQDYF
                     KNKEVGVSEGIENLQAQYRAFQEERDKEKVSTEEIEEAFETFKEKRDAVHEFEVKLTE
                     HQIEKLVDEGIYIKVSFGINQSGLIFIPNYQLDIMEEENQKKYKVYIRETTSYFVYNK
                     EHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERLQEKISEISLLIELTETDKKYQDI
                     KDNLVSEIAELDIKLTQTNEKIATLNKMAEVLINSKSEGSGSQKLARHEFSKLNMTES
                     TTLEQVNEELLKLQQEFGNVLDEYEKTIRKLGQLFKVFDECINKEIMNEI"
     gene            complement(4198..4563)
                     /locus_tag="VUW49_000493"
     CDS             complement(4198..4563)
                     /locus_tag="VUW49_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HET7980460.1"
                     /translation="MVYRYRTNLKKVFLTDLELHQLNERIAKSHCQNFSVYARKVLLN
                     PNMSFVTINTDIYDQLVFELRRIGNNINQIARAINQSHLISQEQLQELRKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            complement(4576..4932)
                     /locus_tag="VUW49_000494"
     CDS             complement(4576..4932)
                     /locus_tag="VUW49_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980461.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVGNYRHFSPFVREKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDMHEVLLIARENHQLNQEHVSILLTCVQELIAE
                     VNQAQPLSREFHEKYM"
     gene            complement(5246..6151)
                     /locus_tag="VUW49_000495"
     CDS             complement(5246..6151)
                     /locus_tag="VUW49_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HET7980462.1"
                     /translation="MNNISFEIQQQIIQCFGLCFHYKDTVVSFMQASGVPNELILRWK
                     SEPKFVWAKNVINELNKTENGRLIIRRIATEFYKMKNIPDEVQDRDRGLDALRKLKWL
                     IGDTQQNKINETFNNSYHRSKQEMKIQLRQQQLQKIEELKTEYYSLFSSENPQKRGYR
                     LEKIVANLFKNSDIDYHESYRNDTNTQQLDGYFRFEGFDYLVEIKWEKDPINSSKIAS
                     LKQKVDTKLTSTRGLFISVNGFRDEVIQDFSNRDSKILFMDGQELSYILENRISLYEA
                     LKVKIIGASKTGNPNVSIISSVNRF"
     gene            complement(6177..9260)
                     /locus_tag="VUW49_000496"
                     /pseudo
     CDS             complement(6177..9260)
                     /locus_tag="VUW49_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(9257..10066)
                     /locus_tag="VUW49_000497"
     CDS             complement(9257..10066)
                     /locus_tag="VUW49_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HET7980463.1"
                     /translation="MEKMHSYIKYLTQEDPIITDEGKQIEILHLDIQDDPEIFEDWAK
                     QFRRNYCSDDELAEMIGFMNISPKEYLENFKLPSDSGIGLSTMSGDFGEILVSDYLQY
                     IEEYVVPRTRYDSKVNKNTSTQGSDVLGYKQDSSNVSNDEVVVIEVKSSASDSSSSKA
                     KNKLQEAINHSDKDFERFSTSIVASYLRLKKSNSDQANVVERFLNITDKPFNVIYGAV
                     AVYSNQSYDIDVIKTVVSKNHRDFQKLRLLVIHSNELMKFIRELYSKASEV"
     gene            complement(10050..10829)
                     /locus_tag="VUW49_000498"
     CDS             complement(10050..10829)
                     /locus_tag="VUW49_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET7980464.1"
                     /translation="MRLEEFSEAEFQKALQRIIRALTRGKTIPGQPKVILLGGQSGAG
                     KTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQEKYGKDSVDYTKGFAGKMVEYLV
                     DELSKQGYHLLIEGTLRTTQVPRQTAQLLASKGYQVSLAVIGTKPELSYLSTLIRYEE
                     LYAINPNQARATPKEHHDGIVEHLVDNLRELEGDKLFDQIQIYQRDRRCVYDSNIDES
                     SAAEVLQNNILFGKWSKVEEEMMKAGHERLREIGRRNGKDA"
     gene            complement(10829..11305)
                     /locus_tag="VUW49_000499"
     CDS             complement(10829..11305)
                     /locus_tag="VUW49_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7980465.1"
                     /translation="MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTE
                     LIDRICQKFNVSYVDIVGEDKMLTPVEDYQLTLKVEVIKERGAAILSQLYRYQDSQDI
                     AFDDKANPWILMSDDLAELINTKIYLVDTFDEIERYNGYLDGIERMLDMVDHRVVA"
     gene            complement(11375..11665)
                     /locus_tag="VUW49_000500"
     CDS             complement(11375..11665)
                     /locus_tag="VUW49_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980466.1"
                     /translation="MKFFQKKTQNQDRFKRLIHRLSEMSDSELTKVEQLLDVVFDPSF
                     KSSERVEISRNDITTEEQSLDRSIQEAKNKLNTEQLEKRIEQFKQSKKPKNG"
     gene            complement(11715..12104)
                     /locus_tag="VUW49_000501"
     CDS             complement(11715..12104)
                     /locus_tag="VUW49_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HET7980467.1"
                     /translation="MSKTWNFDQPLDDVTPTSSNEERAKIAALFHKQGDQPNEEMDYV
                     AAFEKQKKNPEAKVKSKTEEKDKQSQSLKVNITSDYKQHLADTIAQNNKDISACQKQI
                     EELHQLIDEKKIQNKKLQAISVAIDDL"
     gene            complement(12101..12328)
                     /locus_tag="VUW49_000502"
     CDS             complement(12101..12328)
                     /locus_tag="VUW49_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980468.1"
                     /translation="MSVIERLAEKVARQEEKVSRETEKLEHYRDQLQTAMYSTFIKRQ
                     QSSQLSFHEALEQAFGKEVPQHPNYRNEVTE"
     gene            complement(12382..13458)
                     /locus_tag="VUW49_000503"
     CDS             complement(12382..13458)
                     /locus_tag="VUW49_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET7980469.1"
                     /translation="MTRIKTVKQKAILDVAESLGYSFRRLSGHIYEHPDHDSFRIFAD
                     TNTFKWFSRDIQGDVIDFVQLVAGVTFKEAVSYLETGDFEQAKLIEETYQPFQYYLHE
                     EPFQKARIYLKDLRGLSDQTINTFGRQGLLAQATYHSESALVFKSYDHNGTLQAASLQ
                     GLVKNEERHKRGYLKKIMKGSHGYVGISFDIGNPKRLIFCESAIDMMSYYQLHHKQLS
                     DVRLVSMEGLKLAVIAYQTLRLAAEEQGKLAFLDTVKPSRLSHYLLAIQETTTFFKTH
                     SNVITLAVDNDEAGREFCQKLSDKGLPISQDLPQLQGLETKSDWNNIVKQQKGLSLSD
                     IIQTAQAQVNKNHPPPKWEHTLEL"
     gene            complement(13497..14135)
                     /locus_tag="VUW49_000504"
     CDS             complement(13497..14135)
                     /locus_tag="VUW49_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7980470.1"
                     /translation="MLNKVKARFLIGVGGLIAVSFMVMIGYTIGSQSVSKQTQHQIRA
                     EANKLVTKEKQEEKATVLSDDLVNEFLTQYFTKVQLGENNSRIKPYMTDSAFSEEEVN
                     QNKAINQVYKDYMLDYRFESASIYVNTESNVALAEVTYQVTYVSDLSEQQQRTTQTET
                     KTVILSYSKVSDKLLVNQLTIWNGKLEDMKEATDGANSSIPTIQGTTKSENN"
     gene            complement(14231..14521)
                     /locus_tag="VUW49_000505"
     CDS             complement(14231..14521)
                     /locus_tag="VUW49_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980471.1"
                     /translation="MLEQILQSLLIIAAIGLILLVLYRIVKVSGALFLIGLISGLVFI
                     EIYGIYLFFTERYLYTEDLATNGVWSFTGFFIVLNLFLVLSFIMKWWKNRMV"
     gene            complement(14535..14834)
                     /locus_tag="VUW49_000506"
     CDS             complement(14535..14834)
                     /locus_tag="VUW49_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980472.1"
                     /translation="MTQTLEEMRYQLEEWLAQGFTSPEDRANYQTLKEQYEDETLDYS
                     FSKREITGQLELIITSREDDFPSLDEVTKAEYLDLVAQLDELDQEQADYYRKQLA"
     gene            complement(14905..21738)
                     /locus_tag="VUW49_000507"
     CDS             complement(14905..21738)
                     /locus_tag="VUW49_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET7980473.1"
                     /translation="MNQEVLLQMMRATIPRDRALLEAFLYYQAEHFDEEWDSLVYQFM
                     TNRQEFKRPVQVLHFETDVSAFVQASPYDTAHDLLTYTQVFGQTGLQKLDKLSPSEKD
                     LVIEVALFNLATRFQLLDSNGHYQTISPDSLLQKSKGANLVNVYRVANNLADRISRDI
                     EQFLLTYEPEFETRLDETVQDKEETVDVYKTSVHQAISFREEGFLVIASLDVDLSQLD
                     VQTGKTSHLPAYEELSLRRKFDILTYFDQIRNERSKVPSFRRGDFDTEMEMTPVFDGE
                     ELLAYLEADGSPYELNRTLTTVEEKELEKIGQVIRIENQEKLTQVGIDLSQFDPDQVG
                     ILLDAAGRFRLKNADLALLGGYPKASVTQLALATEILQMGLSHEKVEFFFGSQLSLEE
                     LRQVAYAFLHEELSREDAEQFEKDKGIQPDLTLRDWKNKLEKAETKVVVDEELTENPL
                     VQRVLDTYPLGSLVSYKGQDFEVMSVSDARLNGLIRIELVNDFSDIIEQNPVLYVRTW
                     EEVSRALHQPKAEPQTELDDADQELNLFSFLEESVIEHSIQTVGLLEPSGVEEPNNDV
                     IDQLNNQGPVEEVEKSIPEIPVTDFYFPEDLTDFYPRTARDKVETNIAAIRLVKTLET
                     ERRQASPSEQELLAKYVGWGGLANDFFDDYNPKFSKEREELKGLVTDKEYSDMKQSSL
                     TAYYTDPALIRRMWDKLERDGFTGGKILDPSMGTGNFFAAMPKQLREKSELYGVELDT
                     ITGAIAKHLHPNSHIEIKGFETVAFNDNSFDLVISNVPFANLRIADNRYDKPYMIHDY
                     FVKKSLDLVHDGGQVAIISSTGTMDKRTENILQDIRETTDFLGGVRLPDTAFKAIAGT
                     NVTTDMLFFQKHLNKGYVADDLAFSGSIRYDKDNRIWLNPYFDGEYNSQVLGTYEVRN
                     FNGGTLSVKETSDNLIASVQTALNHVKAPREIDRNEVIINPDVLTKQVIDTSIPPDIR
                     ENLGQYSFGYQGSTVYYRDNKGIRVGTKTEEISYYVDEDGNFKAWDTKHSQKQIDRFN
                     ALEVTDSTALDVYVTDDAAKRGQFKGLYKKTVFYEAPLSEKEVARIKGMVDIRNAYQE
                     VIAIQRYYDYDKGEFNDLLGKLNRTYDSFVKRYGYLNSAVNRNLFDSDDKYSLLASLE
                     DESLDSSGKSVIYTKSLAFEKALVRPEKEVKAVHNALDALNSSLADGRGVDFAYMMSI
                     YQVDSKAVLIEELGDYIMPDPEMYLRGELTYVSRQDFLSGDVVSKLEVVDLLVKQDNR
                     DFNWTHYAGLLEAVKPARITLADIDYRIGSRWIPLSVYGKFAQETFMGKAFDLSDQEV
                     VTVLEVSPIDGVITYQSKFAYTYSNATDRSLGVPGSRYDSGRKIFENLLNSNQPTITK
                     QVVEGDKKKNVTDVEKTTVLRAKETHLQELFQDFVAKYPVVQQMIEDTYNSLYNRTVS
                     KVYDGSHLTIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLK
                     ELGMVHKPLYVVPSSLTAQFGQEIMKFFPTKKVYVTTKKDFVRARRKQFVSRIITGDY
                     DAIVIGDSQFEKIPMSREKQVTYINDKLEQLREIKLGSDSDYTVKEAERSIKGLEHQL
                     EELQKLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEM
                     KVRQVQAEHGDRNVVFATGTPVSNSISELYTMMNYIQPDVLERYQVSNFDSWVGAFGN
                     IENSMELAPTGDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKIIAVE
                     SELTEAQKYYLEELVERSDAIKSGSVDPSVDNMLKITGEARKLAIDMRLIEPAYTLSD
                     NEKILQVVDNVERIYLEGAGERATQMIFSDIGTPKSKEEGFDVYNELKALLVDRGIPK
                     EQIAFVHDANTDEKKNSLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWR
                     PSDIVQRNGRLIRQGNMHQEVDIYHYITKGSFDNYLWQTQENKLKYITQIMTSKDPVR
                     SAEDIDEQTMTASDFKALATGNPYLKLKMELENELTVLENQKRAFNRSKDEYRHTVSY
                     CEKHLPIMEKRLSQYDKDIAQSLATKSQDFVMRFDNQAMDNRAEAGDYLRKLITYNRS
                     ETKEVRTLASFRGFDLKMTTRGPSEPLPETVSLTIVGDNQYTVALDLKSDVGTIQRIS
                     NAIDHIIDDQEKSQELVKDLKDKLRVAKVEVEKVFPKEEDYQLVKAKYDVLAPLVEKE
                     AEIEEIDAALAKFSEDTTPQMKQQIALEI"
     gene            complement(21788..22339)
                     /locus_tag="VUW49_000508"
     CDS             complement(21788..22339)
                     /locus_tag="VUW49_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980474.1"
                     /translation="MAWNFDTMKEALSEMEKVNYHEFIKVFLSLELSISDRTILNQVY
                     QDYMDEDDLSLISDELRVKVDGYQDEVKADMTDILEKLYRTGEGSSFIMDLMSSNSLS
                     DTLEQYEVLDSDDYSSLSLETLQAMIQQDLAISSQDYFGDLVHLALQKDLLDQKSYFL
                     QHYVATVMEGIPQERDQRALVLD"
     gene            complement(22323..22514)
                     /locus_tag="VUW49_000509"
     CDS             complement(22323..22514)
                     /locus_tag="VUW49_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7980475.1"
                     /translation="MNPKTIYEKDSDHDGLTDAQELALGTVPLSNDTDGDGQADLEEL
                     QSGHSPLVPQKELCDGLEL"
     gene            complement(22515..27410)
                     /locus_tag="VUW49_000510"
     CDS             complement(22515..27410)
                     /locus_tag="VUW49_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7980476.1"
                     /translation="MTKTCNHHFLVNQEKGEKHVFRKSKKYRTLCSVALGTMVTAVVA
                     WGGTVAHADEVTSSVDTTIQRTENPAINLPEVQPSPVSEQTESLASTGQSNGAIAVTV
                     PHDTVTQAVEEAKAEGVSTVEDSPMDLGNTTSAAETNQQISKAEADAQKQVEAINEVT
                     ETYKADKAAYESEKARIEQENKDLSQAYEGANQTGKETNTWVDTKANDLKKQYADADV
                     TVKEQVVSSGNGTSVLDYTNYRKAVETIQSTNEQAVADYLTKKTKADEIVAKNQVIQK
                     ENEAGLAKAIADNEAIERHNQAGQAAVDSENRAGQAAVDQANQEKQQLVLDRAAEIEA
                     ITKRNQEKEAAARKENEAIDAYNAKEMERYQRDLAEISKGEEGYISEALAQALNLNNG
                     EPQAQHGAITRNPDQIISTGDAMLGGYSRILDSTGFFVYDSFKTGETLSFNYQNLRHA
                     RFDGKKIRRVTYDITNLVSPAGADAVKLVVPNDPTEGFIAYRSDGNGDWRTDKMEFRV
                     VAKYFLEDGSQVTFSKEKPGVFTHSSLNHNDIGLEYVKDTSGKFVPINGSTIQVTDEG
                     LARSLGSNRANDLNLPEAWDTTSSPYAYKGAIVSTVTSGNTYTVTFGQGDMPQNVGLS
                     YWFALNTLPVARTVTPYSPKPHVTVELEPVPEPITVTPDVFTPKTFTPEKPVTFTPKP
                     LEEVVQPTLTLTKVTLPVKPIPKELPTPPQVPTVHYHAYRLTTTPEIMKEVVNSDQAN
                     LHEKTVAKDSTVIYPLTVDALSSNRAQTTSLIFEDYLPAGYLFDLENTQAENGDYEVT
                     FDVSKNFVTLTAKENLLQEVNKDLTKVYQLTAPKLYGSVQNDGASYSNSYKLHLNKGT
                     TNAYTVTSNIVTVRIPGDGETTTLITPDKNNENADGVLINDTVVALGTTNHYRLTWDL
                     DQYKGDRSAKETIARGFFFVDDYPEEVLDVLENGTAVTTLDGQKVSGITVKTYASLNE
                     APKDLQDKLARAKITPTGAFQVFTPDDNQAFYDQYVKTGTSLALLTKMTVKDSLYGQT
                     KTYTNKAYQVDFGNGYETKEVTNTLVSPEPKKQNLNKDKVDINGKPMLVGTQNHYTLS
                     WDLDQYRGIKADNFQIPQGFYFVDDYPEEALLPDETAIQFIASDGKTVSGITVKSYAQ
                     LSEAPKSLQVALSKRNIQPKGAFQVFMPEDPQAFYESYVTKGVNITIVTPMTVLETML
                     NSGKSYENVAYQVNFGQAYETNTVTNFVPKVTPHKSNTNQEGILIDGKTVLPNTVNYY
                     KIVLDYSQYKDMVVTDDVLAKGFYMVDDYPEEALTLNPDGIQILDKDGNRVSGISVST
                     YASLSEAPKVVQDAMAKRQFTPKGAIQVLSSDDPKTFYETYVKTGQTMVITLPMTVKN
                     ELTKTGGQYENTAYQIDFGLAYVTETVVNNVPKLDPQKDVVIDLSHKDDSLDGKEVAL
                     NQIFNYRLVGAMIPSNRATDLFEYGFEDNYDEKHDEYNGVYRSYLMTDVTLKDGSVLK
                     EGTEVTKYTLQQVDTENGLVSISFDKSFLETISDDSAFQADVYLQMKRIATGRVENTY
                     LHTVNGYVISSNTVVTHTPKPEEPSPNQPTPPQPPIEITEPPVPASILPNTGEQESLL
                     GLIGAGILLGTAYGLKKKEEK"
     gene            complement(27550..28548)
                     /locus_tag="VUW49_000511"
     CDS             complement(27550..28548)
                     /locus_tag="VUW49_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET7980477.1"
                     /translation="MLEFKKISKEDLQAIIRNASERLGINEVIVEKDYWVCFVLNYLF
                     TKSEWKDAFTFKGGTSLSKCYDLIKRFSEDIDLILDWQVIGYGKDEPWQSRSNTQQDK
                     FNKESNLKVEDFLVNALIPQMRIDFANLIEDDFQISVDENDPQTVLFEYPTSFQSEYV
                     KQVIRLEIGALAVWTPSEFVEITPDLYKIYPTLFEGDSISVRTVLPERTFWEKATILH
                     HEANRPADSKMPIRYARHYYDIYCLANSIYKNKAYTNRELLEKVVSFKEKFYPRKWAK
                     YEDATIERIRLLPDDYRLKEIESDYNQMKEMFYGSVPSFDEVLTTIANLEKEIHKL"
     gene            complement(28551..29153)
                     /locus_tag="VUW49_000512"
     CDS             complement(28551..29153)
                     /locus_tag="VUW49_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980478.1"
                     /translation="MSNIKAKINSKIDNLPDGAVFISNDFLELADYETIRKNLNRFVN
                     EGTIQRIVNGVYYKPRFIELIGEYESPSINEVAIAIARKYNWTIAPSGNTALNLLGLS
                     TQVPAQWTYISDGRYVNFIIGNTKLVFKRTTNSTISNMSQLTALVIQAIKAIGKDNIS
                     EEQILYLKNRLTISDKEKLLEEGKATSAWIYKVLKKIGES"
     gene            complement(29482..31404)
                     /gene="ltrA"
                     /locus_tag="VUW49_000513"
     CDS             complement(29482..31404)
                     /gene="ltrA"
                     /locus_tag="VUW49_000513"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET7980479.1"
                     /translation="MTKPKNDDKLLKHQKLRYAEYYGMTEIFDDLYSKSQKGFSFKNL
                     MNIIISPENILLAYRTIKRNGGSQTEGVDGVTIKDLENLTQEDFISKVQRRFQYYRPR
                     KVRRVEIPKPNGKKRPLGIPSMWDRVAQQCILQVLDPICEAKFYKHSYGFRPLRSADN
                     AIMDCMYRMNKSHMTYVVDVDIKGFFDEVNHTKLMRQIWTLGIRDKQLLVIIRKMLKA
                     PIVLPNGKVTYPTKGTPQGGILSPLLANINLNEFDWWIGRQWEERDCKELVPQYNSKG
                     TRHKSHVYRKLRTSTTLKEVYIVRYADDFKIFCRNRNEAERTFKAVKLWLKERLNLPI
                     SEEKSQITNLRKKSSEFLGITLKLVSKNNRLVCFSHIASKAKKRIKHQLKQQMKLIQI
                     KGAKETIIREIQKYNSMVIGIHNYYSMATHVSKDLEEIHYQLYLSFKNRLQAKGLTKI
                     GEYKGKDKGLLPYLQSQNIRYLMGYPILPISYVKTRTLKGRNKNLNRYTPEGRKLIHK
                     QQESVETWKLKWLREHSIINKRATVEYNDNRISLFVAQKGKCAVTGQELDMDDIHCHH
                     KRPWLETKDDSYRNLIIVGRDIHRLIHATNEKVIKNLFQLLNLSDTGLTKLNQLRELA
                     GNSPLLKENLNLEQLC"
     gene            complement(32113..34806)
                     /locus_tag="VUW49_000514"
     CDS             complement(32113..34806)
                     /locus_tag="VUW49_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET7980480.1"
                     /translation="MKEDKKLVKQARQNFRSNLKSARMHYRKEVRTLKQTVPKKGRFR
                     KPAQNSLFQDKKTELKQNLLGSQKEAEEKFLKEITYVSPRLLRVKEIKNYRLPQAQER
                     LRTAKKQLSEVKLDEKQKAVNPKFLFQKENPSSKSHFQFHQEKSFDRLSAEKEVRSAK
                     REVKQLKKVRKSKKNSTKVKGGLGLAISESLDLVAQDDDLDGLRTIKDTSLKARRYGR
                     FTYQASKVAVKSGQTSVRFTKTKVAHGKERFHNFKKGKGFTRQKPLKSRRRYQTFLKH
                     ARKHSLAGIKGIAQAIKGSLTFFSAIAGNPLTWIVSGILLILLLMMSFFMSVSGSSVI
                     QQDEMELTKAYTHMTWEDAEHTRTNEKGITYYTKVDDVMVYMNHQYQDYKLDDVKETG
                     GAIYKAFLSQVWTDLNGGDSIKSMSDLYKEPSYKLSDEDQEELKELTEEGNYLALQEL
                     DNPFQGQTDEDSLNMSYRYGYEVIDEKPTLHHHIILEAKEDQVIVAPMDGKVSLDGEN
                     VILTSGKGVNRTKLTLFGIHSGRVSENQQVLAGDIIGQNKDGTGLKVTYQKVDDDTDK
                     LVYVNPAFYFPKVIQVQTTILPTIGQFGGDEFERAKAIYDYLKSEGATNQAIAAILGN
                     WSVESSINPKRAEGDYLSPPIGATESSWDDEGWLSLNGPTIYNGRYPNILKRGLGLGQ
                     WTDTADGSRRHTLLLEYAKGKHQKWYDLGLQLDFMLHGDSPYYSNWLKDFFKNSGSPA
                     SLAQLFLIYWEGNSGDKLIERQTRATEWFYQIEKGFSQPNGGTAQSDPKSLEAVRGDL
                     FENSIPRGGDGMGYAYGQCTWGVAARINQLGLKLKGKNGEKIPIISTMGNGQDWVRTA
                     ASLGGETGTIPQAGAILSFAGGGHGTPTEYGHVAFIGARRF"
     gene            complement(34808..37159)
                     /locus_tag="VUW49_000515"
     CDS             complement(34808..37159)
                     /locus_tag="VUW49_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7980481.1"
                     /translation="MKKLNHLMKQKTSSNDNKQKTKTQKQEIRPSTLNTLAYQGLFQN
                     GLMQVSPSHFSQTYLLGDVNYQTVGLDDKGAIVEKYSDLINSLDDKTNFQLTIFNQKV
                     NLEKFRKSILYPLQEDGFDGYRDELNRMMDANLEAGENNFSAVKLLSFGKSDQTPKLA
                     FRSLSQIGEYFKSGFSEIDVSLGLLDGEERVNVLADMLRGENHLPFSYKDLTLSGQST
                     KHFIAPTYLSFKHKNHIELDDRLLQIVYVRDYGMELGDKFIRDLMQSDLEVMISLHAK
                     GSTKSEAMTKLRTKKTLMESQKIGEQQKMARTGIYLEKVGHVLENNIDEAEALLQTMT
                     QTGDKLFDTVFLIGVLADTEDQLKQALDIIKQVAGSNDMIIDNLTYMQEAAFNSLLPF
                     GKNYLEGVSRSLLTSNIAVNAPWTSVDIQDKGGKFYGINQISSNIISIDRGKLNTPSG
                     LILGTSGAGKGMATKHEIISTKLKEADSDTEIIIVDPENEYSIIGQAFGGESIDIAPD
                     STTFLNVLELSDENMDEDPVKVKSEFLLSWIGKLLDRKMDGREKSLIDRVTRLTYKHF
                     DTPSLVEWVFVLSQQPEQEAKNLALDMELYVEGSLDIFSHRTNIKTDSHFLIYNVKKL
                     GDELKQIALMVIFDQIWNRVVKNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRVR
                     KYGAIPTGITQNVETLLLDANGRRIIANSEFMILLKQAKSDREELVHLLGLSKELEKY
                     LVNPEKGAGLIKAGSTVVPFKNKIPQHTKLFDIMSTDPEKMRT"
     gene            complement(37116..37469)
                     /locus_tag="VUW49_000516"
     CDS             complement(37116..37469)
                     /locus_tag="VUW49_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HET7980482.1"
                     /translation="MNTRVFKDISKVQHRAWLGFTTRQVVFVFPAVAITIMVLGLNLF
                     YWQFGDWFVYGFVFSFTIPLMLFGVYRPNDLPFETYLKYRFHYELTVPDRTFTGRKGD
                     QREKIKPLNETKDLF"
     gene            complement(37531..38385)
                     /locus_tag="VUW49_000517"
     CDS             complement(37531..38385)
                     /locus_tag="VUW49_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HET7980483.1"
                     /translation="MMMNFTSPFVFLASEKVSSDSLFEGFQVDLETTANLVKSLADFN
                     PTVWSYMTAITKGIMQPLGVAILAVVLVLEFSKMAKKIANSGGAMTFEAIAPMIVSYI
                     MVAVVITNTTVIVEAIIALASYVIEQVAGLVTNGGASYDTISGIKGSGIVGKMIIGFF
                     AILIWLVRMVSIMVVNILITIRFIQLYLMIPFAPVTIPTFLSDDWRSVGIGYLKNIMV
                     YAVQGILIFLIVSLVPLFESAGKIAVSNGAGVMESLAIMFGGLVQAILLIIALVGSQR
                     TARSILGM"
     gene            complement(38404..38646)
                     /locus_tag="VUW49_000518"
     CDS             complement(38404..38646)
                     /locus_tag="VUW49_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980484.1"
                     /translation="MNQKLTGFVYGVDASSMFSQAMSLLQKGLIAVGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQITI"
     gene            complement(38664..40445)
                     /locus_tag="VUW49_000519"
     CDS             complement(38664..40445)
                     /locus_tag="VUW49_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HET7980485.1"
                     /translation="MGLAFGYFCHRLTLLYDSLTNAPPMERFAYLLGEGLNQVFNPLW
                     LFSFTRKSLLAFILGVITMSLVYLYVSTGQKVYREGEEYGSARFGTSKEKRFFYSKNP
                     FNDTILTRDVRLTLLEKKKPQFDRNKNLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVD
                     PKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFRYVETENDLNRMLTVYFNNTK
                     GNGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPAFSEIGKLI
                     KLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTLDSVIAVTTAKFA
                     LFNIQSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFSTLTRQA
                     DVDFKGQLPIHVRSYLDEFANVGEIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKE
                     KEAWKTILGNCDSLLYLGGNDEETFKFMSGLLGKQTIDVRSTSRSFGQTGSSSTSHQK
                     IARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHKNWKWLADKESDERWWHYH
                     INPLITEEEIDLSGHTIRDLSTEMSIY"
     gene            complement(40481..40969)
                     /locus_tag="VUW49_000520"
     CDS             complement(40481..40969)
                     /locus_tag="VUW49_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980486.1"
                     /translation="MSSEQQERQAMQYVERSSLFTVRTLLKLLEWSARQALAQDSAYK
                     IGVQKLEELLQSPYAIEAIDVSKDILDKPIDVEKFKELMESEKLPIAISWQKDYLYFY
                     AKDKTLLDHHLDELLKKLMSNPEKLEGLTFDKTLDQEIELAKEKIKVTEPSVVRTKEV
                     TL"
     gene            complement(41046..41630)
                     /locus_tag="VUW49_000521"
     CDS             complement(41046..41630)
                     /locus_tag="VUW49_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980487.1"
                     /translation="MIRIVLFYLAIQLNGLLVSLYLHDYMTIKVLILLQTLLLGVACL
                     EITHHKTVQAKNITLQNRLRWLLLGFACMVAFAVLISFLFPVQTRNQSVLVEVGKQVP
                     HVIFLLFLVNASVLEEIVYRQLLWEKLTFPFVQIGVTSFLFSLAHGPNQLGSWLMYSC
                     LGVTLAVVRLKTDCMTAMTLHLLWNSLVYVLTFL"
     gene            complement(41633..41866)
                     /locus_tag="VUW49_000522"
     CDS             complement(41633..41866)
                     /locus_tag="VUW49_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7980488.1"
                     /translation="MNERFWDNLEIILAEKELTWAELARKVFKGQYVYPSEFNRLYQK
                     LRHYKSNRLMPQTRWVERIVLVLDIDYEDLFKR"
     gene            complement(41875..42258)
                     /locus_tag="VUW49_000523"
     CDS             complement(41875..42258)
                     /locus_tag="VUW49_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980489.1"
                     /translation="MLEVYLGNNDNTNRDLLTILTTYGVTYRCTKACDVNREIILSLF
                     AKTMDCFELLSPRFLRFKRQYTISLNEMIQLILQKPDQNLRLPLIVCQNHVYPAIGLD
                     EVRTFLPRQVKEELFQASLMTQVTG"
     gene            complement(42269..42697)
                     /locus_tag="VUW49_000524"
     CDS             complement(42269..42697)
                     /locus_tag="VUW49_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980490.1"
                     /translation="MNLLHKKSILECTELEERIHQVEINQLLQKILSLPNFDCDFEVT
                     FEDDYHKEMNDPLFYESNLHRISDFLETRDIKNGVDTLLTKDNHLAFRAFGQNYTARG
                     KDGILTTLVTVKCSGEGRMPVDMSHYFSTPEPTVENSLTL"
     gene            complement(42681..44036)
                     /gene="dcm"
                     /locus_tag="VUW49_000525"
     CDS             complement(42681..44036)
                     /gene="dcm"
                     /locus_tag="VUW49_000525"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HET7980491.1"
                     /translation="MKFLDLFAGIGGFRLGMEAQGHECLGFCEIDKFARTSYKAMFKT
                     EEEIEYHDIKEVTDHDIRQFRGQVDVICGGFPCQAFSLAGRRLGFEDTRGTLFFEIVR
                     CAKQIQPRFLFLENVKGLLNHDEGRTFATILSTLDELGYDVEWQVLNSKDFQVPQNRE
                     RVFIIGHSRRYRSRFLFPLRRENSPAHLERLGNINPSKRGLNGEVYLTTGLAPTLTRG
                     KGEGAKIAIPVLTPDRLEKRQHGRRFKDNQDPMFTLTSQDRHGVVVAGNLPTSFDQTG
                     RVFDIAGLSPTLTTMQGGDKVPKILLREELPFLKIKEATKRGYAKATLGDSVNLAYPD
                     STKRRGRVGKGISNTLTTSDNMGVVVAAMEYRQDKWYEVTGIVLDGKLYRLRIRRLTP
                     RECFRLQGFPDWAYERAESVSSKSQLYKQAGNSVTVTVIEAIAREFRRMEEEEKHEPT
                     T"
     gene            complement(44194..45004)
                     /locus_tag="VUW49_000526"
                     /pseudo
     CDS             complement(44194..45004)
                     /locus_tag="VUW49_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(45001..45174)
                     /locus_tag="VUW49_000527"
     CDS             complement(45001..45174)
                     /locus_tag="VUW49_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980492.1"
                     /translation="MQKYEQSSRQLVMCQLMAIMGVDIVKATQLIDEMEQVGLIQFDE
                     FGNVGILVLEGQS"
     gene            complement(45386..46747)
                     /gene="rlmD"
                     /locus_tag="VUW49_000528"
     CDS             complement(45386..46747)
                     /gene="rlmD"
                     /locus_tag="VUW49_000528"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET7980493.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDHVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVECVVLLQR
                     RNV"
     gene            complement(46821..47795)
                     /locus_tag="VUW49_000529"
     CDS             complement(46821..47795)
                     /locus_tag="VUW49_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET7980494.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(47869..48483)
                     /gene="pmp23"
                     /locus_tag="VUW49_000530"
     CDS             complement(47869..48483)
                     /gene="pmp23"
                     /locus_tag="VUW49_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET7980495.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(48483..49457)
                     /locus_tag="VUW49_000531"
     CDS             complement(48483..49457)
                     /locus_tag="VUW49_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET7980496.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(49465..50721)
                     /locus_tag="VUW49_000532"
     CDS             complement(49465..50721)
                     /locus_tag="VUW49_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7980497.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(50783..51214)
                     /locus_tag="VUW49_000533"
     CDS             complement(50783..51214)
                     /locus_tag="VUW49_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980498.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(51216..51815)
                     /locus_tag="VUW49_000534"
     CDS             complement(51216..51815)
                     /locus_tag="VUW49_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET7980499.1"
                     /translation="MDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(51802..52641)
                     /gene="prmC"
                     /locus_tag="VUW49_000535"
     CDS             complement(51802..52641)
                     /gene="prmC"
                     /locus_tag="VUW49_000535"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7980500.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(52641..53720)
                     /gene="prfA"
                     /locus_tag="VUW49_000536"
     CDS             complement(52641..53720)
                     /gene="prfA"
                     /locus_tag="VUW49_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7980501.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(53717..54307)
                     /locus_tag="VUW49_000537"
     CDS             complement(53717..54307)
                     /locus_tag="VUW49_000537"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET7980502.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GIGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKTYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFTPEINKENEEK"
     gene            54431..54613
                     /locus_tag="VUW49_000538"
     CDS             54431..54613
                     /locus_tag="VUW49_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET7980503.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAIVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            54751..56124
                     /gene="mnmE"
                     /locus_tag="VUW49_000539"
     CDS             54751..56124
                     /gene="mnmE"
                     /locus_tag="VUW49_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7980504.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     SQVASHTLNYGHIVDPLTGKVMDEVMIGAMKSPKTFTRENIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQETNRII
                     LLNKTDLPETIETSELPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(56171..57106)
                     /gene="dapA"
                     /locus_tag="VUW49_000540"
     CDS             complement(56171..57106)
                     /gene="dapA"
                     /locus_tag="VUW49_000540"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7980505.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     IPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(57162..58238)
                     /locus_tag="VUW49_000541"
     CDS             complement(57162..58238)
                     /locus_tag="VUW49_000541"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7980506.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(58580..58652)
                     /locus_tag="VUW49_000542"
     tRNA            complement(58580..58652)
                     /locus_tag="VUW49_000542"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58617..58619),aa:Thr,seq:ggt)
     gene            complement(58752..59261)
                     /locus_tag="VUW49_000543"
     CDS             complement(58752..59261)
                     /locus_tag="VUW49_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET7980507.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(59259..59355)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            59384..59815
                     /locus_tag="VUW49_000544"
     CDS             59384..59815
                     /locus_tag="VUW49_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7980508.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            59897..60274
                     /gene="mscL"
                     /locus_tag="VUW49_000545"
     CDS             59897..60274
                     /gene="mscL"
                     /locus_tag="VUW49_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET7980509.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(60316..61410)
                     /gene="hemH"
                     /locus_tag="VUW49_000546"
     CDS             complement(60316..61410)
                     /gene="hemH"
                     /locus_tag="VUW49_000546"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET7980510.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENIKMPYLIKKMLEKAGKLPKE"
     gene            61565..62788
                     /gene="pepT"
                     /locus_tag="VUW49_000547"
     CDS             61565..62788
                     /gene="pepT"
                     /locus_tag="VUW49_000547"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET7980511.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQLIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMENLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(62824..63453)
                     /locus_tag="VUW49_000548"
     CDS             complement(62824..63453)
                     /locus_tag="VUW49_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980512.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLEPTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            63418..63639
                     /locus_tag="VUW49_000549"
     CDS             63418..63639
                     /locus_tag="VUW49_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980513.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(63776..63985)
                     /locus_tag="VUW49_000550"
                     /pseudo
     CDS             complement(63776..63985)
                     /locus_tag="VUW49_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(64072..64335)
                     /locus_tag="VUW49_000551"
     CDS             complement(64072..64335)
                     /locus_tag="VUW49_000551"
                     /inference="COORDINATES: protein motif:HMM:NF016182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET7980514.1"
                     /translation="MLEITLKSPYKFAHILFQPTIVPHGGHYHFIPESDLSAGELAAT
                     YVFNPNDIVRDTGDAYIVRHGVTITIFLSHLLTIPHHTLTRKR"
     gene            complement(64379..64939)
                     /locus_tag="VUW49_000552"
                     /pseudo
     CDS             complement(64379..64939)
                     /locus_tag="VUW49_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(65165..68284)
                     /gene="phtE"
                     /locus_tag="VUW49_000553"
     CDS             complement(65165..68284)
                     /gene="phtE"
                     /locus_tag="VUW49_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET7980515.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSISKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLSIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNSE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(68494..>68901)
                     /locus_tag="VUW49_000554"
     CDS             complement(68494..>68901)
                     /locus_tag="VUW49_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET7980516.1"
                     /translation="KPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLYKPS
                     TDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVETLTGLK
                     SSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT        19556 a        14493 c        12190 g        22662 t
ORIGIN      
        1 tttgtctcta tgctaaagga atccgctata aatacaagta ctatagccgt cggcattcct
       61 cattaacacg acccaatttg attaatcagt gttttcaaac aaaaggtaaa aatgaaatct
      121 ggctggggga tttaacttat attccgacaa aagcaggtac tctttattta tcattatata
      181 tatgtttata gcagaaaaat tgttggttag gcaatgggaa atcgaatgca agataagttg
      241 gtaacgaatg ttttcaatca agcttatcat agagaaaatc tcaaataaga actagtcggc
      301 cataccgatc aaggatctca atatactgga gctagatttc aagaactcct taaacagaaa
      361 agatgcaagt ctagcctgag tcgaaaaggc aatccatatg ataatgcacc catggaatcc
      421 ttttacaaaa cataaaaaga gaacttgtca atgatgccaa tttcgcaact attgaacgaa
      481 atctttaaat atattgaaat ctattacaat cctaaatatc tccattcggc attagggtac
      541 caataactaa tagagtttga aaaaaatagt cactaatcat cgagtccact gaaaaagtaa
      601 gctctacagt tcaactaaaa tgaaaaaaga gggatctatc tcctgcttgg atagattcct
      661 ctttgctttt ccttattttt ggtttattag agttataatc ctcttcatat taaccacaaa
      721 tatggttgtg gcggcttgta actccatgtt taaaagacct ggtgctctag ctacattaag
      781 accatgtctg ttttcaagtt ccgcattttt ggcttctatc ttgtagcgat gtttggcctt
      841 ttctttaaaa tagggcgctt cctgaaacct cttctggaaa atatgttcat cgctcttaag
      901 gggtatcaaa taagtcttag acttagcccc ttctttataa cagcccactt ttgaagggcc
      961 tatttgacag tgcttgatgt caaaatagtg ggtttcctga gccgtccttc ttcctgtctt
     1021 ttccttcttt agtgccatat gcccttctgg acaaacaaag agacctgcgt ccttgttata
     1081 gaaaaactca tcttccttcc tacgaaagcc ttttgaaata gttggatatg gcctagcgac
     1141 tcaatgcaca cgttctttct tggtaaattg catattatcc ttcccagagt aggccctatc
     1201 tccaacaata gcttcaatcg tcacgccatt atccttagat ttgtgataga gttcttctaa
     1261 aacaggacca tcactttttt cacccgatgt caccacacag gcagtaataa tccgttcatc
     1321 ggtcatggca aaatgctctt tgtacccgta aaaggaacta tcggctgact tatgtccaat
     1381 tcttgcttct tctttgacag aggattccaa gtgctctaag tcatcctcta cagcttcttt
     1441 aaggtaattg aattttgtga gatagctggt attccagtca attcgtcatg cttctcaagg
     1501 actgatatta attcttgtgt atatcttaat tcagctacta gagtatcttc ctgaggctta
     1561 ctaggaaatt ctgccttgat atattccgaa tactgataaa tcgttttacg caaggctttt
     1621 gagcgttctc ggaggacttc ttgaggtttc ttgtgattgt aatgaacttt ggtatgagtc
     1681 gcatccacaa tgagaatgtt acttttaatc agaccgagct ctatcgcaat ctgaacagat
     1741 ttagcgatga gcaaatcaag taatcgttca tctttgttcc gaagttttcg gaatttagtc
     1801 aatgaggacg gttcaataac aggagctaga ccaaggaagt atttgaaggt cataccagaa
     1861 aaggagcgct ccaccacatc cacatcagac aacttataga tgtcttttaa taagagatac
     1921 ttgaacatca tgataaggag agagtaggaa cgacatccga agtcaggttg ataattcttt
     1981 tccagctcgt cataaataaa actaaaatca cagagttcca ttagctgtcg taaaaaatga
     2041 gttttgggaa caacaatatc acacagagta gaatagggac taacatcgat tatcaagcat
     2101 accatcacct catctttagt ataccatcat acgatgactt tttcagtgga ctccgaatta
     2161 tcttaactct tgacaagtcc agtgatgcaa tgatagagca tagacaactt taatgatttg
     2221 gggtcgaagc gtaatcgtaa aataggtaaa atcgtctttg aatgcatttt tattaatgtt
     2281 tgttttagaa gttaaaccga tattggcaac cataaacaga aaagatatcc aaaaaaattt
     2341 gagtttgtct tcagtctgat ttatatttca ttcataattt ctttattgat acactcatca
     2401 aaaaccttaa ataattgtcc aagctttctt attgttttct catactcatc aagaacatta
     2461 ccaaattcct gttgtaactt caaaagttct tcattgactt gttctaaagt tgttgattct
     2521 gtcatgttca atttagaaaa ttcatgccta gctagttttt ggctacccga accttcactc
     2581 ttagaattga taagtacttc cgccatctta tttaaggtgg caattttttc atttgtttga
     2641 gtcagtttta tatctagctc tgctatttcg gaaaccaagt tgtctttaat gtcttggtat
     2701 ttcttatctg tctcagttaa ctcaatcaag aggctaatct cagaaatctt ttcttgaagt
     2761 ctttcaacag tcggtcttct gtatggtatt actcgactat cgttggttag ctgcctaatc
     2821 aaggttctcc ctttgatata ctgattctta tccgaatgtt ctttgttata gacaaaatat
     2881 gaggttgtct cacgaatgta gactttatat tttttctgat tttcttcttc cataatatcc
     2941 agttgatagt taggaatgaa aataagacca ctctgattta taccaaagga cactttgata
     3001 taaatcccct catcaactaa cttctcaatt tggtgttctg taagttttac ttcaaattca
     3061 tgaacggcat ctcgtttttc cttaaatgtc tcaaaggctt cctcaatttc ttcagtagat
     3121 accttctcct tatctcgttc ttcttgaaaa gcacggtact gtgcctgtag attttctatt
     3181 ccttctgaaa cacctacttc cttattttta aaataatctt gaaaaaaatt gacatcatat
     3241 aatttctcat cacttatttt tttatgactc agactgaatt gctttccatt ttcttcaagt
     3301 ataaagatta catttttttg ttttaagtca atggttagat tcaattcttt tgattttgtt
     3361 agtaaccctt ctaaagaatt gacacagttc aacaaaaatt ctaaacgact tgcaatctct
     3421 tgcttggcaa aatgcattct aaaaaaatct tcatcatata aatctcgttt actgagttga
     3481 cgtcctcgaa ttggttttgt cattgcccga tccgtcatca tgaatcgact atgcttctga
     3541 ctaaaatcaa tatgaacatg taattgctct gctttttcta aaaaatcatc aaaggatttt
     3601 gactgctgca tcaaaaaata aagacgttgc tttagttcaa acttatggct agtcgctcta
     3661 tatttttgat agtcacggta agagaaacgc ttttcaataa ttttcgcccc agccatttta
     3721 gaaatgcggt ctgaaatcat acgaaggttt cgttccaagg cataattcca tatcaacttt
     3781 ttatctgaat tacagtcaat ggcgttgatt aagatgtgat tatgaatatg atctttgtct
     3841 gtatgagtcg ctacgatgaa acgaaaacgg cctcctgtta attccataat ggtctcataa
     3901 ccaatgcggt taatttcttc aggtgtcaga ttatcttcag gagaaaatga ttgaatgagg
     3961 tgatgggcat gaatattttg ttggtgtttt tcttgtcggt catttctgta ttcgtacaac
     4021 ttgtcattat tggtaaagtt gacattgtac atttctacca tttcttcata gctaggaaag
     4081 tctaagtaat tgctcatacc aaaatcagat actaatttca aattgtccgt tttatcagga
     4141 ttgaggatat acttaattaa acgcctacga tatttttttc cgtgtgtcgc aaaatgctta
     4201 gtgactacca tgaaactcct tcaatctttt cacttccact tgaaattctt tgtccacttc
     4261 cttgatcaac tctccgactc ctttacgtag ttcttgtaac tgttcctggg aaatcagatg
     4321 gctttgattg atcgcacgcg caatttgatt gatgttattt ccaatccgtc tcaattcaaa
     4381 aactaactgg tcataaatat ctgtgttaat cgtgacaaaa gacatattgg gattaagcaa
     4441 cacttttctg gcatagaccg aaaaattttg acaatgactc ttagcaattc gctcattcaa
     4501 ttggtgtaat tctaaatctg ttagaaatac ttttttgaga ttggtacgat aacgataaac
     4561 catagtaacc acctctcaca tatacttttc atggaactca cgactgagtg gctgtgcttg
     4621 attgacttct gcaatcaatt cttgaacgca agtcaataag attgagacgt gctcctgatt
     4681 cagttgatga ttctctcttg caataagcaa aacctcatgc atatctcgac taatctgctc
     4741 aaacttttga gattgccaaa gggagaacca ttctttagca gctaactgtt tatcatccgt
     4801 catcaaaatt ttttctctga cgaatggtga aaagtgacga taattgccaa cctccatcat
     4861 cttttcaatc tgttttagct catcagcggt tagatttacc tcttttcgaa tgtcacgata
     4921 ctgttcagac atctcttttc ctcactttca tcctttctcg ggtaaacccc gaccccgtgt
     4981 gtcatacggg aaatttccta aagcccttgt tcggtctttg cgagctcagg tattgtgtcc
     5041 acaatatccc aaaaatcata tcgccagccg accaaaaccc tctagttttg acagctaacg
     5101 ataagataac ttggtggcta cgcccccaaa cccccagtga aaaatctagg cttgattttc
     5161 ttaggaatga tataagaata gcagggaaga tttggaaaag gtatcacagc atgaaaagaa
     5221 aataagaaaa agagccagat ggctctcaga aacggttaac actgctaatg atagaaacat
     5281 ttggattgcc tgttttagaa gcaccaatta ttttcacttt cagagcttcg tatagactaa
     5341 ttctattttc tagaatataa gataattctt gaccatccat aaataaaatc ttagaatccc
     5401 tattggaaaa atcttgaatc acttcatctc taaagccatt taccgatata aacaatcctc
     5461 gagtagaagt tagctttgtg tcgacttttt gttttaaaga agcaattttt gaggaattta
     5521 tgggatcctt ttcccatttt atttcaacta gataatcaaa accttcaaac ctaaaatatc
     5581 catccagttg ttgagtattt gtgtcatttc gatatgactc gtgataatca atatcactat
     5641 ttttgaataa atttgcaact attttttcta aacggtatcc tcttttttga ggattttcac
     5701 ttgaaaatag cgaataatac tccgttttta gttcttcaat tttttgaagt tgttgttgcc
     5761 tgagttgtat tttcatttcc tgttttgaac gatgataaga attattaaat gtttcgttaa
     5821 tcttgttctg ttgagtatca cctatcagcc actttaattt tcttaacgca tctaatcctc
     5881 tatctctatc ttgaacctca tcaggaatat ttttcatctt ataaaattca gttgctattc
     5941 gtctaattat caatcttcca ttttctgttt tattaagttc atttataaca tttttcgccc
     6001 aaacaaactt tggttctgat ttccatctta atatcaattc attgggaaca cccgaagctt
     6061 gcataaatga aaccacagtg tctttatagt gaaagcataa tccaaaacac tgaataattt
     6121 gttgttgaat ctcaaaagat atattattca ttttatctcc tttaaaatca aatattttac
     6181 atttcattaa ttaactgtat gtaataactt ggatattccg tcaatttacc tttcaattca
     6241 attgatttac gtttgatttc atttctaatt tgattcttac ttacagaatt aatctcttga
     6301 ccaataactt ggaccacaat tctatctgaa aaacctaatt catagatatt gatttctttt
     6361 tccagaggta gcccatattt cagcttcttt attaacatag tcaatacttc caattccccc
     6421 ccaactagtt cttcaagaat attattaatc gcatttaaaa ccattgatgt ttcataccct
     6481 aatccattat cacaaataga gattatgtca gacatatcaa ttggttttga cttacctctt
     6541 ttctcaatac taattgattt ttcagtacaa ctttccaata tttgaaaata agaactgccc
     6601 ttaatccaga gttgagaaat ataatttaga ttttctatac ttaattttct catctccttg
     6661 ttatttgaaa aatctattat tatctcagtt aaaacactta ataaatcttc ttccttctcg
     6721 caagaaagga tagaagtttt attttcctga actcattctt ttaatagctc agattgatat
     6781 aacccaattg agctttttgc aattatttca gtttctacta aactagtaac tattttagat
     6841 tttaccaaga caaataattc ttttattttt tcttgttcct cttcattacc taagaaatat
     6901 ccaaaagttt tttcagctaa actatctgtc tccaattcag tagcatacat agaagcaata
     6961 taattttcaa ttgcaattag ggttttgtca atctggtcta tcttagaatg aaaatcctta
     7021 aaataaggac tgttctgttc ttttaattct gataaaatat tgtttatttt tgttctgtag
     7081 tctacagtac tgtcaaatct ctctttaatc aaactccaat aatcaaattt tatgggattg
     7141 aatcgataat cagttggaac tactgattta aactgtataa tactgagaat attgctttga
     7201 caaccctctg tattaatagg atttaacaat gcttcatagt tttgtttttt tctcttattt
     7261 tttggtgatt tataaatatt gggctctgtt aaaattattg ttccttctgt atattttcca
     7321 gcccttcctg ctctacctat caaattctga aaatcacgaa cttttattgc atctcctgct
     7381 tgatacacac ttgagataat caagtattta ataggtaagt tcacaccttg tgctaaggtt
     7441 gaagtacata ctacattatt tattttactt tttgaaaaag catactctat agcaatgcgt
     7501 atccccatag gtatacctga atgatgtgaa aaaactccca gctgactaca tgcatattca
     7561 acactatcat atcctaaatt ttgaccaagt aagtttccta ttttttcaac ttcatactta
     7621 tctgagtttt ttaagaaatc ggtcagatca taattcctat tatttaagtc tataaatctt
     7681 cgcaaaacaa cattgactgt gtcttttctt ccacaaaaaa ttcctactcc accattatta
     7741 attagcttag tcccataata aatacttata tcatttgcat ttgacagttc tggaaatact
     7801 ttttgttttc gttcttttcc aagcagctct aattcactaa cactaacagt acgtggaaca
     7861 aaaaattctt cttcaaatgt aataggatct atgaaatata attgttcatt actcccgttt
     7921 aaatcactaa acgcaactct tttctctgtt gttttaatcg aattattaga aacgattact
     7981 ccatcttcgt taaaccaccg atttatagct tcactatttg gtattacagc agaaattaaa
     8041 atcttctgta tttcttttgg gaaaatttgt ttaaccgttg acaacagtaa ttcaaaattg
     8101 gttccacgac ttggatcatc aaaaagatgt gcttcatcaa atatacaaag tcctatttca
     8161 tcaataaaac tttgatgatt tctcaataaa aacaacaatt tctctggagt aagtataaaa
     8221 actctttttt taccatcatt aaaaattgag aaatcctgtg gaatctccgg taaatcaaaa
     8281 acttcactaa caatcacatt attctcatca acgaaatgtg cattaatatc tcgatatatt
     8341 tctctacata atgctctaaa aggtgcaaca atcaatgcat tatcaattct atttgaataa
     8401 aatgctgatc ttaaagttag attgattgag gctgttttac ccgaacttgt tggcatctga
     8461 acgattccag atttacctga aaaaattcct tgcttcccta attcgatttg agatggccaa
     8521 agctccttta tgcttcctgt tgattgtata taatcctgcc ataaagaaaa atctaatcct
     8581 gaaaattcag gtaataattt cgttgatgaa ctatttattt ttttacatac aactgctaag
     8641 agacaattta caataattac agaaaaatcg cttccattcc tatatacttt atcttgtaaa
     8701 tctctataaa tatctataga aaactcagtt tttgataaaa tataatttct atatgattca
     8761 actagagtat ttaactcact agagtattta ccttctattg gcgtatgatt caaaaagagt
     8821 tcatccgtta caacgtcaat aaatactttt ataagagtca tacagtcttc taataactgg
     8881 atatcatccg atatatagtt aatcataact ctcgaactac cattataatc gcatagataa
     8941 taagctgtgg tacccaatat tgcaatatag tcttgatatt cagctgaatt tcctaatcct
     9001 gacaccagca atgcatcaaa atattcagca gcattgtgta attgattttt taattgtaaa
     9061 ttaaaactct tacgattgat ataaacttca aaatacttcc tagcagtatc tgcaacaatt
     9121 gctagtgccc caagaaacga tttttgagca tcctctgaaa ttggtaaatg actttctaaa
     9181 ggaatatcaa attcaatcat ctttgccttt gaacgcacta attttgaaaa atataatgac
     9241 cttctcccaa attctatcat acctcactcg ctttcgaata gagttctcta ataaatttca
     9301 ttaattcatt agaatgaatg accaatagtc gtaatttctg aaaatctctg tgattttttg
     9361 aaactactgt cttgataaca tcaatatcat atgattgatt ggagtagact gcaacagctc
     9421 catagattac attaaacggt ttatccgtta tgtttaaaaa tctttctact acatttgctt
     9481 gatcactatt agatttcttt aacctcaaat agctggctac aattgaggtt gaaaatcgtt
     9541 caaaatcttt atcagaatga ttaatagctt cttgaagctt atttttcgct ttagagctac
     9601 tagaatctga tgcagatgat tttacttcaa taactacaac ctcatcattc gacacattcg
     9661 aagaatcttg cttatatcct agaacatcac ttccttgagt tgaagtattt ttattaactt
     9721 tactatcata acgagtccta ggaaccacat attcttcaat atactgaaga taatcagata
     9781 ctaatatttc tccaaaatct cctgacatcg ttgaaaggcc aataccacta tccgagggta
     9841 atttaaaatt ttcaagatac tctttagggg agatattcat aaaacctatc atttcagcaa
     9901 gttcatcatc agaacaataa tttcttctga attgttttgc ccaatcttca aaaatttctg
     9961 ggtcatcttg aatatctaag tgaagaatct caatctgctt accttcatca gtaataatag
    10021 gatcttcttg agttagatac tttatataac tatgcatctt ttccattcct tctacctatc
    10081 tccctcaatc gttcatgccc cgccttcatc atctcttcct ccaccttact ccatttaccg
    10141 aagaggatgt tgttttgaag aacttctgct gctgaactct catctatgtt agaatcataa
    10201 acacaacgtc tatccctttg ataaatttga atttggtcaa agagtttatc gccttctaac
    10261 tctcttaggt tatcaaccaa atgctctaca ataccatcat gatgttcttt aggggttgct
    10321 ctggcctgat taggatttat tgcgtaaagt tcctcataac gaatcaaggt gctgagataa
    10381 gaaagctctg gtttggtacc aattacagct aaagaaactt ggtaaccttt agaagctaat
    10441 aattgagcag tctgacgagg aacttgagtg gtacgcaaag tcccttcaat taacaaatgg
    10501 tacccttgct tactgagttc atcaaccaga tactctacca tttttcctgc aaatcccttg
    10561 gtatagtcca cgctgtcctt gccatacttt tcttgcaggg ctaagtaatt gggatgctga
    10621 gaacggtagc tgtcaccatc gatgataatg atattacctt gaaattcctt ctgcttgata
    10681 cgatgtatcg ttgtcttacc tgccccgctt tgtccaccaa gcaagataac tttcggttga
    10741 cctggaatcg tctttcctcg ggttaaggcc cgaatgattc gctgcaaagc cttctgaaac
    10801 tcagcctcac tgaattcttc caatctcatt aagctaccac ccgatgatcc accatgtcta
    10861 acatccgctc aataccgtct aaatagccat tgtaacgctc tatttcatcg aaagtatcga
    10921 caaggtaaat tttcgtattg attaattcgg ccaaatcatc actcatcaaa atccaaggat
    10981 tggctttatc atcaaaagca atatcttgac tatcctggta tctataaagc tgtgataaaa
    11041 tggctgctcc acgttctttt atcacttcta cttttaaagt cagttggtaa tcttcaacag
    11101 gtgttagcat cttatcctct cctacaatat cgacataaga aacgttaaat ttttgacaaa
    11161 tgcggtcaat gagttctgtt gaaacggtgc tggttccatt ttcgtagcga ctcaggctat
    11221 tacgtgaaat cccgaccatt ttcgcaaatt ctggttgtgt taaatcgtgt gttcgacgta
    11281 gtgatttaat attgtctcca atcatagata aagacctcct catatttgct ttcattatac
    11341 cacaaaaaac ggacaacgca ccaaatttgg tgcgttatcc gtttttaggt ttcttcgatt
    11401 gcttaaactg ctctattcgc ttctccaact gctcagtatt cagtttgttt ttagcttcct
    11461 gaattgacct atcaagactt tgttcctcag tagttatatc gtttcgagat atttctactc
    11521 tctcactaga cttgaaactt ggatcaaaga ccacatctag gagttgttct actttggtta
    11581 gttcactatc tgacatctct gatagtcgat gaatcaatcg tttaaatcgg tcttgattct
    11641 gtgttttctt ttgaaaaaat ttcattaact tctccttaaa ttgtaaaaag gtagcccgca
    11701 taggactacc tgacttataa atcatcaatg gctactgaaa tagcctgtaa ctttttattt
    11761 tggattttct tttcatcaat caattgatga agttcttcaa tttgcttttg acaagctgaa
    11821 atatccttgt tgttttgtgc aatggtatct gctaagtgtt gtttgtagtc actagtgata
    11881 tttactttca atgactgact ttgcttatct ttttcttcag tctttgattt tactttagct
    11941 tctggattct ttttttgctt ttcaaaagca gccacataat ccatttcttc atttggctga
    12001 tcaccctgtt tatggaagag tgctgcgatt ttagctcgct cttcatttga agatgttggt
    12061 gtcacatcgt ctagtggctg atcaaaattc catgttttac tcattctgta acctcatttc
    12121 tgtaattggg gtgttgtggg acttctttac caaaggcttg ctctagtgct tcatgaaatg
    12181 acaactgact agattgttgc cgtttgataa aggtgctata cattgcagtt tgtagttggt
    12241 cacgatagtg ttccaatttc tccgtctcac gtgagacctt ttcttcctgc ctagctactt
    12301 tttcagctag acgttcaatc acactcatag ggtgtcctcc ttgtcacatt ctagattatg
    12361 atagcggact tgattttgtt atcacaattc caatgtatgc tcccatttag gtggaggatg
    12421 attcttatta acttgtgctt gggctgtttg gataatatca cttaaggata gtcccttctg
    12481 ttgtttcaca atattattcc aatccgactt tgtttcaagt ccttgcaatt gtggtaaatc
    12541 ctgagaaatc ggaagtcctt tatctgacag tttctgacaa aattctcttc ctgcttcatc
    12601 gttatcaaca gccaatgtta tgacatttga atgggttttg aaaaaggttg tcgtctcttg
    12661 tattgccagt agataatggc taagtctgct tggttttact gtatctagaa atgccaattt
    12721 cccctgttct tctgctgcca gacgcaaggt ctgataagca atcactgcaa gttttaagcc
    12781 ttccattgac accaggcgaa catctgatag ctgcttgtga tgaagctgat aataactcat
    12841 catatcgata gcggattcac aaaaaatgag tcgcttggga ttaccaatat cgaaactaat
    12901 accgacatag ccatgagagc ctttcatgat tttcttcaaa taaccacgtt tgtgtctttc
    12961 ttcatttttg acgagacctt gaaggcttgc tgcctgtaaa gtcccattat gatcatagct
    13021 tttaaacacc aatgctgact ccgaatgata agtagcttga gcaagcaatc cttgtctgcc
    13081 aaaggtatta atagtctgat cacttaggcc acggaggtct tttaagtaaa tacgtgcttt
    13141 ctgaaacggt tcttcgtgca aataatattg aaacggttga tatgtttctt ctatcaactt
    13201 agcttgttca aaatctccag tttcaagata ggacacagcc tctttaaaag taacacctgc
    13261 tactaattga acaaagtcta tcacatcccc ttgtatatct cttgaaaacc atttgaaagt
    13321 attggtatcc gcaaaaatcc gaaaggaatc atggtctggg tgttcataaa tgtgtcctga
    13381 taatcgtcta aaagaataac ccagactttc agccacatct aaaatagcct tttgttttac
    13441 tgttttaatt cgtgtcatct tgtctcccta tcaaaaaagc agaagactga ctcctgctag
    13501 ttattctcac tttttgtagt tccttgtatc gttggtatgc tggaattggc tccatctgtt
    13561 gcttctttca tgtcctccag tttcccattc caaatggtca actgattaac tagtagtttg
    13621 tcggaaactt tggagtagga taatataacc gtcttggttt ctgtctgagt ggttcgctgt
    13681 tgttgctcac ttaaatcaga tacataggtc acttggtagg taacttccgc aagtgcaaca
    13741 ttgctttctg tattgacata gatactggct gattcaaatc ggtagtcaag catataatct
    13801 ttgtaaactt gattgatcgc tttattctga tttacttcct cttctgaaaa tgccgaatcc
    13861 gtcatgtagg gtttgatacg actgttattt tctcctagct gaaccttggt gaaatattga
    13921 gtgagaaatt cattgacaag atcatctgat aagaccgttg ctttttcttc ctgcttttcc
    13981 tttgtcacaa gtttattggc ttctgctcgt atttggtgct gagtttgctt tgaaacggat
    14041 tgtgagccaa tcgtatatcc aatcatgacc ataaaactga ctgcaattaa acctcctact
    14101 ccaatcagaa atcgagcttt cactttattt aacatggctt actcctttat tagtgatagc
    14161 ctcatcataa caagattagc ttggagattt tatcacaaga aaaaggacta ccaatcggta
    14221 atcctccgtg ttagaccatt ctatttttcc accacttcat aataaagctt aaaacgagaa
    14281 atagattcaa aacaataaaa aatcctgtaa aactccaaac accattggtg gctaaatctt
    14341 ctgtataaag gtatctctca gtaaagaaga ggtaaattcc atagatttca ataaagacta
    14401 atccactgat gagtccgata agaaacaaag cacctgaaac tttcacaatc cgatatagga
    14461 caagcaatat tagtcctatt gctgcgataa tcaaaaggct ttgtagaatt tgttccaaca
    14521 tcttacacct ctttctaggc taattgcttg cgataatagt cagcctgttc ctggtcaagt
    14581 tcatcaagtt gggcaaccaa atcaaggtat tccgctttcg ttacctcatc taagcttgga
    14641 aaatcatcct cacgacttgt gatgatgagt tccagctgtc ctgtgatttc acgctttgag
    14701 aagctataat caagcgtttc atcttcatac tgttccttta aagtttggta gtttgcccta
    14761 tcttctggac tcgtaaagcc ttgtgccaac cattcttcaa gttgataacg catttcttct
    14821 agtgtttggg tcatgatcta ttctcctttt gtgatttctt gacttcattt taccgcgttc
    14881 aataatattt gtcagcattt tttcttatat ctcgagtgct atttgttgct tcatttgggg
    14941 tgttgtatct tcactaaact tggccaaagc tgcatctatc tcctcaatct ctgcttcttt
    15001 ttcaaccaag ggagctaaaa catcatactt agcctttaca agctgataat cttcttcctt
    15061 gggaaagact ttttcaactt ctactttggc tactcgtagt ttatctttta aatcctttac
    15121 cagctcttgc gacttttctt ggtcatctat aatatggtca atggcattac taatccgttg
    15181 aatggttcct acgtctgatt tcaaatcaag agcgactgta tattggttat caccgacaat
    15241 cgttaaagag acggtctctg gtaagggctc actaggacct cgtgtagtca tttttaagtc
    15301 aaatcctcta aagctcgcaa gtgttctgac ttccttggtc tctgagcggt tataggtaat
    15361 gagttttcgc aggtagtccc ctgcctctgc acgattatcc attgcttgat tatcaaatct
    15421 catgacaaaa tcttgcgact tcgttgccaa agattgggca atatctttat catattgact
    15481 caaacgtttt tccataatag ggaggtgctt ctcgcaatag gaaactgtgt ggcggtattc
    15541 atcttttgag cgattaaaag ctcgtttttg attttctaaa actgtcagtt cattttccaa
    15601 ttccattttg agtttgagat aggggtttcc agtggctaaa gccttaaagt cagaagcggt
    15661 catggtttgt tcatcaatgt cttcagctga ccgcacaggg tcttttgagg tcataatctg
    15721 agtgatatat ttgagcttat tctcctgtgt ctgccagagg taattatcaa aactcccttt
    15781 ggtaatgtag tgataaatat ccacctcttg gtgcatattc ccttgtcgaa tcagtcgtcc
    15841 atttcgctgt acaatatcag aaggcctcca gggaacatct aagtggtgga cagctttcat
    15901 gcgtgattgc acgtttagac ccgttccacc tttttccgta gatgccatga gaatccgtac
    15961 ttccccacta ttgacctttc gtgataggga atttttcttc tcatcggtat tggcatcatg
    16021 cacaaaagca atctgttctt tgggaatccc tcgatcaacc agcaaagctt tcagttcatt
    16081 ataaacatca aaaccttctt ccttactttt aggtgttcca atatcagaga aaatcatctg
    16141 agtagccctt tctcctgctc cttcaaggta gattcgctcg acattatcaa ccacttgaag
    16201 gattttctca ttgtctgata aggtataagc aggttcaatc aaccgcatat caatggctag
    16261 ttttctggct tcacctgtga ttttaagcat attatcgaca gacgggtcaa cactgcctga
    16321 tttgatagcg tctgaccgct ctaccagttc ttctaaataa tatttttgag cctctgttaa
    16381 ttcactttct accgcaataa tcttagcttc aggaacgggt aaatcaagca tgtctgaggt
    16441 ctgaatatct gcagtctcct tgtaaatccg catgagttca ggaaggttga caaacttctt
    16501 aaaccgtttc ttaggttggt atttatcacc agtcggggct aactccatag aattttcaat
    16561 attcccaaaa gctccaaccc acgaatcaaa attagatacc tggtatcgtt ctaagacgtc
    16621 tggttgaatg taattcatca tggtatagag ttcgctaatg gagtttgaga ctggtgttcc
    16681 agtcgcaaaa acaacatttc tatcgccatg ctccgcctga acctgtctga ccttcatctc
    16741 catatccacg ttctttttag aagtggtatt ggtaatgcca gcgacattgc caagtcctgt
    16801 gattggacgg atattcttga agtgatgagc ttcatccaca aaaagaaaat caatgccaag
    16861 gttttcaaat tcaataaagg tatctcgctc tagtttttgg agttcttcca actggtgctc
    16921 tagtcccttg attgaacgct ctgcttcttt gaccgtataa tcagaatcac ttcctagctt
    16981 gatttctctg agttgctcca gcttgtcatt gatataggta acctgcttct ctcggctcat
    17041 cggtattttt tcaaactggg aatctccaat gacaatggca tcatagtctc ctgtgataat
    17101 acgcgataca aactgcttac gtctcgctct tacaaagtcc ttcttagtgg tcacatagac
    17161 tttcttggtt gggaaaaatt tcatgatttc ttgaccaaac tgagcagtga gacttgacgg
    17221 caccacataa agaggtttat gcaccatccc taattctttc aatttgaaac cagctccgag
    17281 catagtcaag gtttttccag accctacctc atgggctagt agggcacgtt tttcctccac
    17341 aatccgttga atggcattct tttggtgagg acgtaaggaa atattctggg cgagtccatc
    17401 aatggttaaa tggctaccat cataaacctt agaaactgta cgattataga gactattata
    17461 ggtatcttca atcatctgtt ggactactgg atattttgct acaaaatctt gaaagagttc
    17521 ttgtaggtgc gtttccttgg cacgcaggac cgttgttttc tcaacatccg tcacattctt
    17581 tttcttatct ccttcgacaa cttgttttgt gatggttggt tgattggaat tcaggagatt
    17641 ttcaaaaatt tttcgaccac tatcataacg agaacctggt acacctaaac ttctatccgt
    17701 tgcgttggaa taggtgtagg caaactttga ttggtaagta ataactccat caatgggact
    17761 gacttcaagg acagtcacca cttcttggtc agagaggtca aaagcttttc ccataaaggt
    17821 ttcttgggca aattttccgt aaacagacaa gggaatccag cgtgaaccaa tccgataatc
    17881 aatgtctgcc aaggtaatac gtgctggttt aaccgcttct agaagtcctg cataatgcgt
    17941 ccagttaaag tcacgattgt cttgtttgac gagtaggtcc accacttcta acttgctaac
    18001 gacatcaccg gacagaaagt cttggcgaga aacataagtc aactcacctc tcaaatacat
    18061 ttctggatct ggcataatat agtcccctaa ttcctcgatc aaaactgcct tagaatcaac
    18121 ctgataaatg gacatcatat aagcaaaatc aacaccacgt ccgtctgcta aactggaatt
    18181 taaggcatca agggcattgt ggacagcttt gacctctttt tcaggtcgta ccagagcctt
    18241 ctcaaaggca agggatttgg tatagataac agactttcca ctcgaatcca aactttcatc
    18301 ttccaaacta gcaagaagcg aatatttatc atcactatca aaaagattac gattcactgc
    18361 actgttcaaa tacccatagc gtttgacaaa actatcatag gtacgattga gcttgcctaa
    18421 taagtcgttg aactcgcctt tatcatagtc ataatagcgt tgaatggcta tgacttcttg
    18481 ataggcatta cgaatgtcca ccattccttt gattcgtgct acttctttct cagataaggg
    18541 agcttcatag aaaactgtct ttttatataa ccctttaaac tgaccacgtt ttgctgcatc
    18601 atctgtaaca taaacatcta gagcagtgct atcagttact tctaaggcat taaagcgatc
    18661 aatctgcttt tgggaatgct tggtgtccca ggctttgaag ttaccatctt catcgacata
    18721 gtaactgatt tcttccgtct tggtccccac tcgaatgcct ttgttatctc gatagtaaac
    18781 tgttgaaccc tgatacccaa aactgtactg acctagattt tctctaatat caggtggaat
    18841 ggaggtatca atcacttgtt tggtcaatac atctgggtta atgatgacct catttcgatc
    18901 aatctctctt ggggccttaa catggtttag agcagtttgg acactggcaa tcaagttatc
    18961 actagtctcc ttaacagaaa gtgttccacc gttaaagttt ctaacctcat aggttcctag
    19021 cacttggcta ttgtattctc catcaaagta aggattgagc caaatacggt tgtccttgtc
    19081 ataacgaata gaccctgaaa aggctaagtc atctgccaca tagcccttgt tcaagtgttt
    19141 ctggaagaac aacatatccg ttgtgacatt ggttccggca atggccttaa aggcggtatc
    19201 aggcaagcga acccctccaa gaaagtcagt tgtctcacga atatcttgta aaatgttttc
    19261 ggttcgtttg tccatggtcc ctgttgaaga gataatcgct acttgtccac catcatggac
    19321 taaatcaagc gactttttga caaagtaatc atgaatcatg taaggcttat cgtacctgtt
    19381 atccgcaatt cgaagattgg caaacggcac atttgaaatc actaaatcaa agctattgtc
    19441 gttaaaagct accgtctcaa aacccttaat ttcaatatga ctattgggat gaaggtgttt
    19501 ggctatagct cctgtaatgg tatctaactc tacgccatat agctcactct tttctcttaa
    19561 ctgtttaggc atagccgcaa agaaattccc tgttcccatg gaagggtcta ggattttacc
    19621 acctgtaaaa ccatctcttt ccaacttatc ccacatccga cggataaggg caggatctgt
    19681 gtaataggct gtcagagagg actgtttcat atctgaatac tctttatctg tgactaagcc
    19741 tttcagttct tctcgttcct tagaaaattt tggattatag tcatcaaaga aatcattggc
    19801 aagtccaccc caacccacat acttggcaag gagttcttgt tcacttggac ttgcctgtct
    19861 gcgttccgtt tcaagtgttt taaccaaacg aatagccgca atgtttgtct caaccttatc
    19921 cctagcagtc ctaggataaa agtccgtcaa atcttctgga aaataaaagt ctgttactgg
    19981 aatctctgga attgatttct ctacctcctc aacaggacct tgattgttta gttggtcaat
    20041 aacatcattg ttaggttcct ctacaccact tggttctaag agaccgacag tctgaataga
    20101 gtgctcaata actgattctt ccagaaatga aaaaaggttt aattcttggt ctgcatcgtc
    20161 taactctgtt tgtggttctg cctttggctg atgaagtgcc cgactgactt cttcccaggt
    20221 cctcacataa agaactggat tttgttcaat gatatccgaa aagtcattga ctaactcaat
    20281 ccgaatcaaa ccgttcaatc gagcatcgct gaccgacatg acctcaaagt cctgtccctt
    20341 ataggaaacc aatgacccca gaggataggt gtccaatact ctctgaacca gtggattttc
    20401 cgtaagttct tcatcaacca ctactttcgt ctcagctttc tctagcttgt tcttccaatc
    20461 tctgagagtt aaatctggct gaattccttt atctttttca aattgctccg catcttctct
    20521 gcttaattct tcatgtaaaa aggcataagc aacctgtcgc aactcttcta aagaaagttg
    20581 actgccaaag aaaaattcta ctttttcatg acttagcccc atctggagta tttctgtcgc
    20641 aagggctagt tgagttaccg aggctttggg atagccacct aataaagcaa gatccgcatt
    20701 tttcaaacga aaacgacctg ccgcatccaa taaaataccg acttggtctg gatcaaactg
    20761 agataaatca atccctacct gagtcaattt ctcttgattt tctatcctaa tgacttgtcc
    20821 aattttttct aattcctttt cttcgactgt agttagcgtt cgattcagct catagggact
    20881 accatcagct tctagatagg caagtaattc ctcaccatca aagactggtg tcatttccat
    20941 ctcagtatca aaatcacctc gtctaaaact tgggactttt gaacgttcat ttcgaatttg
    21001 gtcaaaatat gttagtatat caaatttacg tcgtaaagat aactcttcat aagctggcag
    21061 atgacttgtt tttcctgttt gaacatctag ttgagacaaa tctacatcca aactagcaat
    21121 aaccagaaaa ccctcttctc gaaaagatat tgcctgatga acacttgttt tgtacacatc
    21181 aacagtttcc tctttgtctt gaactgtttc atcaagtctt gtttcaaact caggctcata
    21241 agtcaagaga aactgttcaa tatctcggct gatccgatcc gctaaattat tagccacacg
    21301 ataaacattg accaaattgg ctcccttact cttttgtaag agtgaatccg gcgatatggt
    21361 ttggtagtgt ccattggaat ccaataattg aaaacgagtg gccagattaa acaaggccac
    21421 ttctatcacc aagtcttttt cagacggcga tagtttgtcg agtttttgga gaccagtttg
    21481 gccgaatact tgtgtatagg tcaatagatc atgagcagta tcataaggac tagcctggac
    21541 aaaagctgaa acatctgtct caaagtgaag tacctgaaca ggtcttttga attcctgcct
    21601 attagtcata aactgataaa cgagactatc ccactcctca tcaaaatgct ctgcttggta
    21661 atataaaaat gcctcaagca aggctctatc acgaggaatg gtggctctca tcatttgtag
    21721 taagacttct tgattcattt ttccctccca ttttaaaagg accgagagtg tttctcagcc
    21781 cattgcgtta atctaggacc aaggctcgtt ggtctctttc ttgtggaatt ccttccatta
    21841 cagttgccac atagtgttgt aagaaataac ttttctggtc tagtaaatct ttttgcaagg
    21901 caagatgaac cagatctcca aaataatcct gactggaaat ggccaagtcc tgttgaatca
    21961 tggcctgtaa ggtttcaaga ctaagtgatg aataatcgtc actatccaaa acttcatatt
    22021 gttccaaggt gtctgaaaga ctatttgagg acattaaatc cataataaaa ctagagcctt
    22081 cacctgttcg gtacaatttt tccagaatgt cagtcatatc agcttttact tcatcctgat
    22141 aaccatccac tttaactcgt agctcatcac taatcaagga caggtcatct tcgtccatat
    22201 aatcttgata aacttgattg aggattgttc tatcagaaat acttaattcc aaagagagaa
    22261 agactttgat aaactcgtgg taattgacct tttccatttc agacaaagcc tctttcatgg
    22321 tgtcaaagtt ccaagccatc acacaactcc ttttgtggga ctagtggtga atgtccagat
    22381 tgtagctctt ctaaatcagc ttgaccatca ccatctgtgt cattgctcaa aggaacggtt
    22441 cccagagcca gttcctgagc atctgtcaaa ccatcatggt ctgaatcctt ttcataaatg
    22501 gttttcggat tcatctactt ctcctccttt ttctttagtc cataagctgt accgagtaaa
    22561 attccagctc caatcaagcc caaaagggat tcttgttccc ctgtatttgg caaaatgctt
    22621 gctggaacag gcggttcagt aatttcaatc ggtggttgag gtggggtagg ttgatttgga
    22681 cttggttctt caggtttagg tgtatgtgtt acaactgtat ttgagctaat gacatagcca
    22741 ttcactgtat ggagataggt attctccacc cgaccagtcg caatccgttt catctgaagg
    22801 taaacatctg cctgaaaggc tgaatcatca gagatagtct ctaagaaaga tttatcaaat
    22861 gaaattgaca cgaggccatt ttctgtatcc acctgttgca aggtatattt cgtgacttcc
    22921 gttccttctt ttaagacaga accgtctttg agggtgacat ccgtcatcag atagctgcgg
    22981 taaacaccat tgtactcatc atgcttttca tcatagttat cttcaaaacc atattcaaat
    23041 agatctgtcg cacgattgct tggaatcatc gctccaacca agcgatagtt aaagatttga
    23101 ttcaaggcca cctctttccc atcaaggcta tcatctttat gagacaagtc aatcaccaca
    23161 tccttttgtg ggtctagttt gggaacgtta ttaactactg tttccgtgac ataggccaaa
    23221 ccaaaatcaa tctgataggc tgtattttca tactgaccac ctgttttggt cagctcattt
    23281 ttaaccgtca tcggaagcgt gataaccatg gtttgaccag tcttcacata ggtctcataa
    23341 aaggttttgg gatcatcgct actaaggact tgaatggctc ctttaggtgt aaactgtcgt
    23401 ttagccatgg catcttgaac aactttcgga gcttctgata aacttgcgta ggtgctgaca
    23461 gagataccag atacacgatt gccatcctta tccaaaatct gaatgccatc aggatttagg
    23521 gtaagagctt cttctgggta atcatctacc atgtaaaatc ccttggcaag aacatcatcc
    23581 gtcactacca tgtccttgta ttgactgtaa tccaagacaa ttttgtaata attgactgta
    23641 ttaggaagga cagtctttcc atcaattaaa atgccttctt gattggtatt agacttgtgt
    23701 ggagttactt taggtacaaa attggtcacc gtattggttt cataagcttg accaaagttc
    23761 acctgatagg ccacgttttc ataagacttc cctgaattaa gcatggtttc caaaaccgtc
    23821 atcggagtga ctatggtaat gttcacaccc ttggtcacat aagattcata aaaggcttgt
    23881 gggtcttcag gcatgaaaac ttgaaaagct cctttaggct gaatattccg tttggaaagg
    23941 gctacttgta aagatttagg agcttctgat aattgagcat aggacttcac agtgattcct
    24001 gaaactgttt tgccatcaga tgcgataaac tgaatagctg tttcatccgg caataaagct
    24061 tcttctggat aatcatccac aaagtaaaat ccttgtggaa tctgaaagtt gtctgcttta
    24121 atccctcggt actggtccaa gtcccatgag agagtatagt gattttgagt tcccactagc
    24181 attggcttcc cattgatgtc cactttgtct ttattgaggt tttgtttctt aggttctgga
    24241 gaaacaaggg tgttggtcac ttccttggtt tcatagccat ttccaaaatc aacttggtag
    24301 gccttgtttg tataggtctt tgtctgaccg tagagactat ctttaaccgt cattttggtc
    24361 agtaaagcta aagatgtacc tgttttgaca tactggtcat aaaatgcttg gttgtcatcc
    24421 ggtgtaaaaa cttgaaaggc acctgtcggt gtaatcttgg cacgagctaa tttatcttga
    24481 aggtctttag gggcttcatt taacgaagca taggttttaa ccgttatacc tgataccttt
    24541 tgaccatcaa gggtagtaac agccgtgcca ttttccaaca catcaagcac ttcctctggg
    24601 taatcatcca caaagaagaa gcctcgtgca attgtctctt tagcagaacg atctccctta
    24661 tactggtcca aatcccaagt caaacggtag tggttggttg tgccaagggc tacgaccgta
    24721 tcgttaatga gaacaccatc cgcattttca ttgttcttgt caggtgtaat aagtgtagtt
    24781 gtctccccat cacctggtat acgtaccgtc acaatatttg aagtgactgt ataagcattt
    24841 gttgtcccct tgtttaagtg gagtttgtag ctattggaat agcttgctcc atcattttga
    24901 acagaaccat agagttttgg agcagtcaat tgataaacct tggttaaatc tttgttcact
    24961 tcctgcaaca agttttcctt tgcggttaag gtcacaaagt ttttagatac atcaaaagtc
    25021 acttcgtagt ccccattttc agcctgtgta ttttccaaat caaagagata ccctgcaggc
    25081 aagtagtcct caaaaatgag actagtcgtt tgggcacgat tggacgataa ggcatcaact
    25141 gttaagggat aaatcaccgt agaatctttt gcgacagttt tctcatgaag attagcttgg
    25201 tcactattga ccacttcttt cataatctca ggagttgtcg tcaaacggta agcatgataa
    25261 tggacagtcg gtacttgtgg tggcgttgga agttcttttg gaataggttt gacaggtaag
    25321 gttaccttgg tcaaagttag agtaggctgc accacttctt ccaaaggttt aggcgtgaag
    25381 gttactggct tttctggtgt aaatgttttt ggagtaaaga catctggtgt taccgtaata
    25441 ggttctggaa caggttcaag ctccaccgtt acatgaggtt tgggactata aggtgttact
    25501 gtccgtgcaa ctggtagagt atttaaggca aaccagtaag acaatccaac attctgtggc
    25561 atatcgcctt ggccaaaggt taccgtatag gtattacctg atgtaaccgt tgagacaatg
    25621 gcacccttat aggcataagg gctagatgtc gtatcccatg cttcaggtaa attcaaatca
    25681 tttgcacggt ttgaacccaa agaacgagcc agaccctcat cggtcacttg gatggttgaa
    25741 ccattaatag gtacaaattt cccagaagta tctttgacat attctagtcc aatgtcatta
    25801 tgattgaggg aagaatgtgt gaagacacct ggtttttctt tggagaaggt aacttgtgaa
    25861 ccgtcttcca aaaaatactt agcaactaca cgaaactcca tcttatccgt tcgccagtca
    25921 ccgtttccgt cactacgata ggcaatgaag ccctcggttg gatcatttgg tacaaccaat
    25981 ttcacggcat cagctccagc tggtgacaca agattggtaa tatcataagt cacacgccta
    26041 atctttttcc catcaaatcg tgcatgtcga agattttgat agttaaaact aagggtctcg
    26101 cctgttttga agctatcata gacaaagaat cctgttgaat ccaaaattct tgagtagcca
    26161 cccagcatgg catcgccagt tgaaatgatt tgatcaggat ttcgggtaat ggccccatgt
    26221 tgtgcttgtg gctcaccatt attgaggttg agagcttgag caagggcttc agaaatataa
    26281 ccttcttcac cttttgaaat ctcggccaag tcacgttgat agcgttccat ttctttggca
    26341 ttgtaggcat caatcgcttc attttctttt ctagctgcgg cttctttttc ctgattgcgt
    26401 ttagtaatgg cttcaatctc agctgctcga tctaaaacta attgttgttt ctcctgatta
    26461 gcctgatcaa ctgcggcttg acctgcacga ttttcagaat ctacagctgc ttgacctgcc
    26521 tgattatgcc tttcaatggc ttcgttatct gctattgcct tagcaagtcc agcttcattt
    26581 tctttttgaa tgacctgatt ttttgcaaca atttcatctg ccttagtttt ctttgttaga
    26641 taatccgcta cagcttgttc gttagttgat tgaatggttt caacagcctt gcgatagttt
    26701 gtatagtcaa gtacagatgt cccgtttcct gatgaaacca cttgttcttt tactgtcaca
    26761 tcagcatctg catactgttt ttttaggtca tttgctttgg tatcaaccca agtatttgtc
    26821 tctttaccag tttggttggc cccttcatag gcctgtgaca gatccttatt ttcctgttca
    26881 atacgagctt tttccgattc gtatgccgct ttatcagctt tgtaggtttc agtaacttca
    26941 ttgatagcct caacctgttt ttgggcatct gcttctgctt ttgaaatttg ttggttggtt
    27001 tctgccgcag aagttgtgtt ccccaaatcc attggactat cttcaacagt agaaacacct
    27061 tcagcctttg cctcttcaac tgcttgtgtt accgtatcgt gtggtacggt gacagcaatc
    27121 gcaccattag attggccagt tgaagctaaa ctttcagtct gttcagaaac tggacttggc
    27181 tgtacttctg gtaaattgat agctggattt tccgttcgtt ggatggttgt atcaacggat
    27241 gatgtgactt catcagcatg tgcaaccgtt cctccccagg caacaacagc ggttaccatg
    27301 gttccaaggg caaccgaaca taacgttcga tattttttac tcttgcgaaa gacgtgtttc
    27361 tcgccttttt cctgattgac aagaaagtga tgattacatg ttttggtcat aaagtgtcct
    27421 tcttttcttt tttctatagc aacaaactgt cactctttcc tcatcatata aaagaaggct
    27481 tgattttgtt atcacaaaaa ttttgacaaa agaaaaaagc cacccacagg tgactaaaat
    27541 aactgatgtc taaagtttat ggatttcttt ctctaaatta gcgatagttg ttaacacttc
    27601 atcaaaagat ggcacagacc cataaaacat ttctttcatc tggttgtaat cagattcaat
    27661 ttctttcaat ctgtaatcat ctggaagcaa tctaattctt tcaatagttg cgtcctcata
    27721 ttttgcccat tttcgaggat agaatttttc cttaaatgaa accactttct ccaataactc
    27781 tcgattagta taggctttat tcttataaat tgaattggca agacagtaaa tatcgtaata
    27841 atgccttgca tagcgtatag gcattttact atctgctggc ctatttgcct catgatgtaa
    27901 aatagtagct ttttcccaaa aggttctctc tggtaaaaca gtcctaactg aaatactatc
    27961 tccctcaaat aacgtcgggt aaatcttata taaatcagga gtaatctcta caaattcaga
    28021 aggtgtccag acagctagtg cacctatctc taagcgaatg acttgcttca catactctga
    28081 ttggaaactt gttggatatt caaaaagtac tgtttgtggg tcattttcat caacactaat
    28141 ttgaaaatca tcttcaatta aattcgcaaa atctatcctc atctgcggaa ttaatgcatt
    28201 cacaagaaag tcttctactt ttaaatttga ttccttattg aacttatctt gttgcgtatt
    28261 agaacgagat tgccatggtt cgtctttacc atatccaatt acctgccaat ccaagattaa
    28321 atcaatatct tctgaaaatc gcttaattaa atcatagcac ttagaaagac ttgtgccccc
    28381 tttaaatgta aatgcatcct tccattcact ctttgtaaag agatagttga gtacaaaaca
    28441 aacccaatag tctttctcaa caatcacttc attgatacca agtctttccg aagcatttct
    28501 aataatggct tgaaggtctt ctttggaaat ttttttaaat tctagcatct ttaggattct
    28561 cctatttttt tcaagacttt ataaatccag gcactggtag ccttgccttc ctcaagaagt
    28621 ttttctttat cacttatagt taacctattc ttaagatata aaatctgttc ttctgaaata
    28681 ttatcttttc caatagcttt gatggcttga ataactagcg cagttaattg cgacatattt
    28741 gaaatggtac tatttgttgt tcgcttgaac acaagtttcg tatttccaat tataaaattt
    28801 acataccgtc catcagaaat ataagtccat tgagctggaa cttgtgtcga caaccctaat
    28861 aaatttagag ctgtgttacc tgaaggtgca attgtccaat tatattttct agcaatggca
    28921 atagccactt catttattga aggagactca tattctccaa tcaactcaat aaaccttggt
    28981 ttgtaatata caccgtttac aattcgttga atcgttcctt cgttaacaaa tcgattcaag
    29041 ttttttctaa tggtctcata atcagcaagc tctagaaaat catttgaaat aaagactgcg
    29101 ccatctggta gattatcaat cttactattt attttagctt ttatgttact caattcctca
    29161 ccaccttgtc ccaaatatta tacaacattt gggacaaaat gacaactatc tttctgtcta
    29221 ttatttcgtc gtatacgcaa aactcaagct gctatccact ccagataatt tacggaaggt
    29281 atagttggga ttgccattgt aattggtttc ggagataagg aatgagccat ctgggtaaac
    29341 tttctatagc gataggtaaa actttgatac tatctccatc ttttcccccg cttcacaccg
    29401 tgcgtgcgac tttcaccgca cacggcgttc catcaatagt gtattatcac gattttgctt
    29461 tatcgtgctg tattaatgtg attaacataa ttgttctaag ttcaaatttt ctttcaataa
    29521 tggtgaattt cctgcaagtt cgcgcaactg atttaatttg gtcagtcctg tatcactcaa
    29581 attcaaaagc tgaaagagat ttttgataac cttctcattt gttgcgtgaa tcaggcgatg
    29641 aatatctcgt ccaacaatga taaggttcct ataagagtca tcctttgttt ctaaccacgg
    29701 tctcttatgg tgacaatgaa tatcatccat atccaattcc tgaccggtta cagcacattt
    29761 ccctttttga gctacaaata aagaaattcg attatcattg tattcaactg ttgcacgctt
    29821 atttataatt gagtgttctc tcagccattt taatttccat gtttcaacag attcctgttg
    29881 tttatgaatc aattttctcc cctcgggagt atacctattc agattcttat ttcttccttt
    29941 tagtgttctc gtcttgacat agctaattgg aagaattgga taacccatta agtaccgaat
    30001 attttgagat tggaggtagg ggagaagtcc tttatctttt cccttgtatt ctcctatttt
    30061 tgtcagacct ttcgcctgca atcgattttt aaaagagaga taaagctgat aatgaatttc
    30121 ttctaaatcc ttactcacat gtgttgccat actataatag ttatgaatac cgataaccat
    30181 actattatac ttctgtattt cacgaataat tgtttcttta gcacctttaa tttgtatcaa
    30241 tttcatctgt tgtttcagtt gatgtttaat acgtttcttg gcttttgatg caatatgaga
    30301 gaaacaaacc aaacgattat tcttagaaac tagctttaaa gtaatcccaa ggaattcact
    30361 gcttttcttt ctcagattgg taatttgaga tttttcctct gaaataggga ggttaagtcg
    30421 ttcttttaac cacaatttta cagccttaaa cgttcgctct gcctcattcc tatttcgaca
    30481 aaagatttta aaatcatccg catatcggac aatataaact tcttttagcg tcgttgaagt
    30541 tcgcagcttt ctgtaaacat gtgacttgtg tcttgttccc ttggaattat attgaggaac
    30601 taattcctta caatctcttt cttcccattg tcgtcctatc caccaatcaa attcattgag
    30661 attgatattg gcaagtagtg gagataagat acctccttgt ggtgtgcctt tagttggata
    30721 tgtcactttt ccatttggaa gaacaattgg tgcttttaac atttttcgaa taatcactag
    30781 aagttgtttg tccctgatac ctaatgtcca gatttgacgc attaacttag tatgattcac
    30841 ttcatcaaaa aagcctttaa tatcaacgtc aacgacataa gtcatatgac ttttattcat
    30901 acgatacata cagtccataa tagcgttatc agccgacctt aagggtctaa atccataact
    30961 gtgtttgtaa aatttcgctt cacagattgg atccaacact tgtaaaatac actgttgggc
    31021 cactctgtcc cacatcgagg gaatgccaag tggtcgtttc ttcccatttg gcttaggaat
    31081 ctctactctt ctgacttttc tgggtctata atattgaaat cttctttgaa ctttagagat
    31141 aaagtcttct tgggtcagat tttctaaatc tttaatggta acaccatcta ctccttcggt
    31201 ttgactacca ccattccgct ttatggtacg ataagcgagc agtatattct cgggagatat
    31261 gataatgttc attaagttct taaaactgaa tcccttttga cttttggaat ataaatcatc
    31321 aaatatctcg gtcatgccat agtattccgc ataccttagt ttttgatgtt ttaatagttt
    31381 gtcatcattt tttggtttcg tcatggtcta tcactcctct ctttggattc gtgacctttt
    31441 atgtcttaca agaatccatg aaatgataaa gttcataaca atacactatt gactagtggc
    31501 tgttcctcca gtatcattac aacactttca aaggtcgtgc caccactctc actagagatt
    31561 aaggaaagtt atgattttct ttccaatgtt tctctggttg tgcaacctcc acatctctag
    31621 ctttcgacgt tcccttatct ttatcaatac taaacttagg tgcctagcta taccccttca
    31681 actttgatat gcctgtaaca taacaaggaa tttcatatcg tcgattctta ctcttcctca
    31741 agttgaaaca acttacgttg ttatacgcat ttctacgtac tcgccttcgg agcgtacatt
    31801 cgctggttcg tcagtgctca tgcacattct caccctattt agtttttaat gactcccgac
    31861 ctatcagata tatattccac ctctggaaca ctttcgattc ttaagtcctt aagaatgacg
    31921 gtatctttca gccgacttca ccgagcttca aacctttacc tttgcacaag ataaacgcat
    31981 gtcggagtat tagagaaatc gtttcagggc gttacccctt cattccaatt ttcaagataa
    32041 gagttctcca ttctattcaa atttaacctg attatcaggt tgatttttca agaacgtgtc
    32101 taaccttttc agttagaaac gtctcgcacc gataaaagcc acatgtccgt attctgtcgg
    32161 tgtgccatgt cctcctcccg caaaagagag aatagctcct gcttgtggaa ttgtgcctgt
    32221 ttcaccacca aggcttgcgg ctgtacgcac ccaatcctgg ccattaccca tggtactgat
    32281 aatcggaatc ttctcaccat ttttaccttt gagttttaaa cccagttggt tgatacgggc
    32341 tgcgactccc caagtacatt ggccgtaagc ataacccata ccgtcaccac ctcttggaat
    32401 agagttctca aagaggtctc cacgtacagc ttcaagagat tttggatcgc tttgtgccgt
    32461 cccaccattt ggctggctaa agcctttttc aatttgatag aaccactcgg tcgctcttgt
    32521 ttgtcgctct atgagtttat ctcctgaatt gccttcccag taaatcagaa agagttgggc
    32581 aagactggct ggactacctg aatttttgaa gaagtccttt aaccaattgg aataataagg
    32641 actatcccca tgcaacatga agtccagttg taaccccaaa tcataccact tttgatgttt
    32701 tcctttggca tattccagca acaaggtatg tctgcgtgac ccatctgcgg tatccgtcca
    32761 ttgtcctaag cccaaaccac gtttgagaat atttgggtaa cgtccattgt atatagttgg
    32821 accattaagt gagagccatc cctcatcatc ccacgagctt tctgtcgcac caataggggg
    32881 agatagatag tcgccctccg cacgttttgg attaattgag gattctaccg accaatttcc
    32941 taagatagct gcgatagctt gattggtcgc accctcactt ttgagatagt cataaatcgc
    33001 cttggctctc tcaaactcat caccaccaaa ctgaccgata gttggaagaa tggtggtctg
    33061 aacctggatc acttttggaa agtagaaagc tggattaaca tagaccaact tatccgtgtc
    33121 atcatcaacc ttctgatagg tgacttttag gcccgttcca tccttgtttt gaccaataat
    33181 gtctcctgcc aagacttgtt gattttcact tactctacct gaatgaatgc caaacaaggt
    33241 tagtttagtc ctattcactc ccttaccaga tgtcagaata acgttctctc catcaagaga
    33301 taccttacca tccatcggag ccacaatgac ttggtcttct tttgcttcta agataatatg
    33361 gtgatgaagt gttggtttct catcaatgac ctcataacca tatcggtagg acatgtttaa
    33421 gctatcctca tccgtctgtc cctgaaaggg attgtccaat tcttgaaggg ctaaatagtt
    33481 gccttcttcg gtcaactcct ttagttcttc ctggtcctca tcagacagct tgtagctagg
    33541 ttctttatac aggtcagaca tggatttaat agaatcacca ccatttaagt ctgtccacac
    33601 ttgactgaga aatgctttgt aaatcgcacc acctgtttcc ttaacatcat ctaacttgta
    33661 atcttgatat tgatgattca tatagaccat aacgtcatca accttggtgt aataggtaat
    33721 ccctttttcg ttggttcttg tatgctccgc atcttcccac gtcatgtggg tataggcctt
    33781 ggtcaactcc atctcatctt gttgaatgac actgcttcct gaaacactca tgaaaaagct
    33841 catcatcaaa aggagtatca agagaatgcc tgagacaatc caagtcaaag gatttcccgc
    33901 aattgcagaa aagaaggtca gacttccttt aatggcttgg gcaatcccct taattcctgc
    33961 tagactgtgc tttctggcat gctttaaaaa ggtttggtag cgtcttcttg atttaagcgg
    34021 tttctggcgt gtgaatcctt ttcccttttt gaagttgtgg aatcgttcct ttccatgagc
    34081 gactttggtc tttgtaaaac gcacactagt ctgtccactt ttcaccgcca ctttactagc
    34141 ttgataggta aatctgccat agcgtctggc tttcaaacta gtatccttta tggttcttag
    34201 accatctaga tcatcatcct gtgctaccag gtcaagcgat tcagatatag ctaagcctaa
    34261 tccaccttta accttggtag agtttttctt agactttcgg actttcttga gttgtttgac
    34321 ctcacgtttg gcagacctca cttctttttc agcacttagt cgatcaaatg atttttcttg
    34381 gtgaaattga aaatgagact tcgaggaagg gttttctttt tggaagagaa acttgggatt
    34441 gaccgccttt tgtttctcat ctagcttcac ttctgacaat tgtttttttg ccgtccgcaa
    34501 acgctcttgg gcttggggaa gtcgataatt cttaatttcc tttaccctta acagtctagg
    34561 agacacatag gtaatttcct ttagaaactt ctcttctgct tccttttggc tacctagcaa
    34621 attttgtttt aactctgttt tcttatcttg aaacagagaa ttttgggctg gttttcgaaa
    34681 tcgtcctttt ttaggaacag tctgttttaa ggtcctaact tctttccgat aatgcatacg
    34741 agctgatttt aagttgcttc gaaagttttg cctagcttgt ttgactagct ttttatcctc
    34801 tttcatctca tgtcctcatt ttttcagggt ctgtactcat gatgtcaaag agcttggtgt
    34861 gttgtggaat cttattctta aacgggacaa cggtggaacc ggccttgatt aggccagcac
    34921 ctttttcagg attgaccaag tatttctcta gttccttgga aagtccaagc aaatgcacca
    34981 actcttcgcg atcgctctta gcctgtttta atagaatcat gaactcgcta ttggcaataa
    35041 tacgtctgcc attggcatcc aaaagaagtg tctccacatt ctgagtaatg ccagttggta
    35101 tcgccccata cttacggaca cgactccaga gtttaaagaa gaaatcagag gcatatttgt
    35161 ccaataagag aagctgcatt tcatcaaagt aaatccaggt cttcttccct agtttttggt
    35221 ttttcaccac acgattccaa atctggtcaa agataaccat gagggcaatt tgttttaatt
    35281 catcgcctag ctttttgaca ttgtaaatca gaaaatgact atctgtcttg atattggtcc
    35341 gatgcgagaa aatatctagc gatccttcca catagagttc catatccagt gccaagttct
    35401 tagcttcctg ttcaggttgt tgagataaga caaagaccca ttcgaccaaa gatggtgtat
    35461 caaaatgctt ataggtaagt cgtgtcacac ggtcaatcag cgacttttcg cgaccatcca
    35521 ttttgcggtc caagagcttt ccaatccaag acaagaggaa ttcggattta accttgactg
    35581 gatcctcatc catattctca tcggatagtt ctaaaacatt taagaaggta gtggaatctg
    35641 gtgcaatatc aatactttct ccaccaaagg cttgaccgat aatgctgtac tcattttctg
    35701 ggtcaacaat aataatttcg gtatcgctat ctgcttcctt gagcttagta gagatgattt
    35761 catgttttgt cgccatccct tttccagctc cagaagtccc taaaatcaaa cctgacggag
    35821 tatttaactt gccacggtca atactgatga tattacttga gatttgattg atgccataaa
    35881 atttcccacc cttgtcctga atatcaacgg aagtccaagg tgcattcacg gcaatgtttg
    35941 aagtcaatag agaacgagaa actccttcga gatagttctt cccaaatggc aagagactat
    36001 taaaagcagc ttcttgcatg taggtcaagt tgtcaataat catatcattt gaccccgcca
    36061 cttgcttgat aatatcaagg gcttgtttga gttggtcttc agtatccgcc agcacgccaa
    36121 ttagaaatac ggtgtcaaac agtttatctc ctgtttgggt catggtttga agaagagctt
    36181 ctgcttcatc gatattgttt tcaagaacat ggccgacctt ctccaaatag atgccagtcc
    36241 gagccatctt ttgttgttcc ccaatctttt gcgattccat caaggttttc ttggttcgta
    36301 acttagtcat agcttcagac ttagttgagc ctttggcgtg taggctaatc atcacttcca
    36361 aatctgactg catgaggtca cgaataaatt tatcccctag ctccataccg taatcacgaa
    36421 catagacaat ctgtaataat ctatcgtcca attcaatatg attcttgtgt ttaaaggaaa
    36481 ggtaggttgg cgcaataaag tgtttggtgg attgacctga cagggttaaa tccttgtaag
    36541 agaatggtaa atgattttca cctcgtaaca tatccgcaag gacattcact cgctcctctc
    36601 catcaagtaa accaagagat acatcaatct ctgaaaagcc actcttgaaa tattccccaa
    36661 tctgtgacag ggaacgaaaa gctagttttg gtgtttggtc gctcttgcca aatgaaagca
    36721 acttgacagc tgaaaagtta ttctcacccg cttctaagtt ggcatccatc atgcgattca
    36781 attcatcacg gtagccatca aacccatctt cctgtaaggg atagagaata ctcttacgaa
    36841 atttttccag attgactttt tgattgaaaa tagtcagttg gaaattggtc ttatcgtcca
    36901 gtgaattgat taaatcagaa tatttctcca caatagctcc cttatcgtct aagccaactg
    36961 tttggtaatt gacatctcct aagagatagg tttgtgaaaa atggcttggg ctgacctgca
    37021 tgagtccatt ttgaaagaga ccttgatagg ctaacgtatt tagggtagaa ggtctgatct
    37081 cctgcttctg tgttttcgtc ttttgcttat tgtcattaga agaggtcttt tgtttcatta
    37141 agtggtttaa ttttttcacg ttggtctcct tttcttccag taaatgtgcg gtctggcacc
    37201 gttagttcat aatgaaaacg atacttgaga taggtttcaa atggtaaatc attgggtcga
    37261 tagaccccaa ataacatgag tggaatggta aagctaaaaa caaaaccata gacaaaccaa
    37321 tccccaaatt gccagtaaaa taggttcaag cccaatacca taatagttat tgcaacggct
    37381 ggaaatacaa aaacaacctg tcttgtggta aatcccaacc atgcacggtg ttgaacctta
    37441 gaaatgtcct taaagacacg agtgttcata aatgttctcc aatctagata agctataaaa
    37501 agaggaggcg ggcaatttac tcccatcctc ctacatcccc aaaatacttc gggcggttcg
    37561 ttgactgcca actaaggcaa tgattaacaa gatggcctgt accaaaccac caaacataat
    37621 ggcgagtgat tccatgactc cagctccatt tgatacggca atctttccag cagattcaaa
    37681 caagggaaca agagatacaa tcagaaaaat caaaattcct tgtacggcat agaccataat
    37741 gtttttgagg tagccaatcc caacacttct ccagtcatca ctaaggaagg tcggaatggt
    37801 aacaggggca aaaggaatca ttagatagag ttggataaag cgaatggtaa tcaagatatt
    37861 gacaaccatg atactaacca ttcgcaccaa ccaaatgaga atcgcaaaaa agccaataat
    37921 catttttcct acaattccag aacccttgat gcctgagata gtatcgtaac tagcaccacc
    37981 atttgtgaca agccctgcca cttgttcaat aacataagag gcaagggcaa taatggcttc
    38041 cacaataacc gtcgtattgg taatcacgac cgcgaccata atgtagctga caatcatagg
    38101 tgctatggct tcaaaggtca tcgcaccacc tgagttggcg attttcttgg ccatttttga
    38161 gaattcgagt acgagaacaa ccgcaagaat agctactccc aagggctgca taatcccctt
    38221 ggtaatggct gtcatgtaag accacactgt cggattaaag tccgctagtg acttaaccaa
    38281 gttggcagtt gtttctaaat caacttgaaa cccttcaaaa aggctatcgc tagatacttt
    38341 ttcagaggct agaaatacaa aaggtgacgt aaaattcatc atcatgtcat gtgctgtcct
    38401 cctctatatg gtaatctgtg tcacaaaagc accagcggca cctaccatga caccaccaac
    38461 aatttctaaa atggcatttc ggacacctgg tccaccatct ttaatgttgg ttgacaggtt
    38521 gataatgccc ataacaacaa gaaaggcccc tactgcaatt aagccttttt gtaatagaga
    38581 catagcttgg gagaacatgg agctggcgtc cactccgtaa acaaagcctg ttagtttttg
    38641 attcatattt ttcctcattt ctattaatat attgacattt ctgtgcttaa atcccttatt
    38701 gtatggcctg acaaatctat ctcttcctca gtgattaggg gattgatgtg gtagtgccac
    38761 cagcgttcat cagattcctt atctgcaagc catttccaat tcttatgttt gagtggaaaa
    38821 tatttcttgg ttcgaaagac aggcactcct gcaattcgta cgaggcattc atctcgtttc
    38881 atattcccta cttcatctgc cgtcattaag tcacgggcaa ttttctggtg agaggtggaa
    38941 cttgagcccg tctgaccaaa tgaacgactg gtacttctga catcgatagt ttgtttgcct
    39001 agaagtccac tcatgaactt aaaggtttcc tcatcattcc cacccaaata taagagactg
    39061 tcgcagttcc ctaatatggt tttccaagcc tctttttcct tatacagtcc ttggagttgg
    39121 gcaatatttt gcaagattgg tactagactc atattcctag agcgaactgt tgaggtttgt
    39181 tcagcaaagt ctggaatttc tccgacattc gcaaactcat ccagatagct tcgaacatga
    39241 atcggcaatt gccctttaaa gtccacatca gcctgtctgg tcaaagtaga gaaaaccgta
    39301 gagaaaaata aagcagatag gaaacgaaaa gtcgtatcat tgtctggaat tacaagatag
    39361 accatggtct tttgagtgcc ccacgttttc aaatccatag tgtctctttg cgtcaaatca
    39421 atcactgatt ggatattaaa aagggcaaat ttagctgttg taaccgcaat caccgaatcc
    39481 aacgtcttat ccttatagtt ttggaaatcc gcccaatttc gcatggtaaa gttttcgtgg
    39541 ccatatttct tagcatagtc ttcaaacaag acttcaagga cacttttgtc ctgattgtct
    39601 ccctttgata agagtttgat gagtttccca atctcagaaa aggctggata acggccacgc
    39661 ttacgtcttg cttcctgctc ttgcttggaa cttcctggag gattgtaaaa atccaccaaa
    39721 taagaggcaa tagctcgcac caaggtcata gaagcctcat cccaaaaggg atcactgcga
    39781 gaaccatttc ctttggtatt attaaaatag accgttaaca tgcggttcaa atcattttca
    39841 gtttctacat agcgaaaggg attaaaacca tctgaattgg tcatattaac caagtctaaa
    39901 accttgactt gataaccgtt ttctaaaaag agcttgcctg ttttttcagc caaatggtcc
    39961 ttaggatcta ccacaatatt ggaacagtta agttggataa ggttaggctt cacaaagcga
    40021 aaggtctttc ctgcaccaga acccccaatg acaattagat ttttatttcg gtcaaactgg
    40081 ggtttcttct tttccaacaa ggttagacga acatcacgag tcaaaatcgt gtcattgaaa
    40141 ggattcttac tgtaaaagaa tcgcttttcc ttactggttc caaagcgtgc agaaccgtat
    40201 tcttcccctt ctcgatagac cttctgtcct gtcgatacat aaagatagac tagcgacatc
    40261 gttataactc caaggataaa ggcaagaagg gattttcgag taaagctaaa tagccataaa
    40321 ggattgaaaa cctgatttaa cccctctcct aagaggtagg caaaacgttc cataggtggt
    40381 gcattggtta aactatcata gagtagggtt aggcggtgac agaaataacc aaaggcaagt
    40441 cccaagaatc caaagactaa tgcttttcat ctgaaataca ttacaaggta acctccttgg
    40501 ttctaactac cgaaggttct gtcactttta ttttctcttt ggccaattca atctcttggt
    40561 ccaaggtttt atcaaaagtt agcccttcta acttttctgg attagacatc agtttcttta
    40621 ataattcatc taagtgatgg tccagcaagg tcttatcttt ggcataaaag tagagatagt
    40681 ctttttgcca actaatagca atcggtagtt tttctgactc catcaactcc ttgaatttct
    40741 ccacatcgat tggcttatcc agaatgtctt tactgacatc aatagcttca atggcgtaag
    40801 ggctttgaag gagttcttcc agtttttgaa caccaatctt ataagctgaa tcttgggcta
    40861 aggcttgcct agccgaccat tctaagagct ttaagagggt tctaacagta aataaactac
    40921 ttctttccac gtattgcatc gcttgacgtt cttgttgttc agatgacatg atgacttcct
    40981 cttctttgaa ttccctatca aacataaggt gcttccataa tacagaagca ctcttgattt
    41041 tggtatcaca aaaaggtcaa gacataaacc aaactattcc aaagtaaatg taaggtcatt
    41101 gccgtcatac aatctgtttt caaacgaaca acagccaagg tcaccccaag acagctatac
    41161 atgagccaac tccctaactg atttggtcca tgggctaaag aaaagagaaa actggtcact
    41221 cctatttgta caaaagggaa tgtcaatttt tcccacagca attgcctata aacaatctct
    41281 tcaaggacac ttgcattgac cagaaacaat aaaaagataa catgtggaac ctgctttcct
    41341 acttcaacta gtaccgattg attcctagtc tgaactggaa aaagaaaact aattaagact
    41401 gcaaaagcaa ccatacacgc aaagccaaga agcaaccatc ttaggcgatt ttgtaaggta
    41461 atattttttg cttgaacagt tttgtgatga gtaatctcta gacaagctac acctaaaaga
    41521 agcgtttgta gcaggattaa gaccttaatc gtcatatagt catgcaggta taaactcaca
    41581 agaagaccgt taagctgtat ggctagataa aacaacacta tccttatcat tgtcacctct
    41641 tgaataaatc ttcataatca atatctaaga ctagaacgat tcgctcaacc catcttgtct
    41701 gtggcattag acgattggat ttgtaatgcc gtaatttttg atagaggcga ttaaactcac
    41761 ttggataaac atattgacct ttgaatactt tgcgagctag ttctgcccaa gtgagttctt
    41821 tttctgccag aatgatttct aaattgtccc aaaatcgttc attcatcttc acacctaccc
    41881 tgtcacttgt gtcatcaggc tggcctgaaa cagctcttct tttacctgtc tgggaagaaa
    41941 ggttcgcact tcatctaacc caatagctgg atagacatga ttctggcaga caatgagagg
    42001 caaacgaagg ttttggtctg gcttttggag aatgagctgg atcatctcgt ttaaactgat
    42061 ggtgtattga cgcttaaagc gaagaaatcg tggcgacagc aactcaaaac aatccatggt
    42121 tttggcaaag agtgacagta tgatttcacg gtttacatca catgcttttg tacaacggta
    42181 agtcacacca taggtcgtca aaatggttag taagtctcga tttgtattgt cattatttcc
    42241 tagatatacc tcaagcatag gctcctcctt atagggttag gctattttca actgttggtt
    42301 ctggagttga gaaatagtga ctcatatcaa cgggcatccg tccttcaccg gagcacttaa
    42361 ccgtcaccag agtcgttaaa ataccatcct ttcccctagc agtataattt tgaccaaagg
    42421 cacgaaaggc caagtgattg tctttcgtca atagtgtatc tactccattt ttaatatccc
    42481 tagtttccaa aaaatccgaa atccgatgaa gattggattc gtagaatagg ggatcattca
    42541 tctctttgtg gtaatcatct tcaaaagtca cctcaaagtc acaatcaaaa ttggggagcg
    42601 acaatatctt ttggagtagc tgattgattt caacttgatg aatacgttcc tctaattcag
    42661 tacactcaag gatacttttc ttatgtagta ggttcatgtt tttcttcctc ttccattctt
    42721 ctaaattctc ttgcaatggc ttcaataact gtgacagtca cactattacc agcctgtttg
    42781 tatagttggc tcttactaga aacactttct gctctttcat aagcccaatc aggaaaacct
    42841 tgaagtctga aacactctct tggtgtcagt cgtctaattc tcaggcgata gagttttccg
    42901 tctaagacaa tccctgtgac ttcataccac ttatcctgtc gatattccat agcagcaact
    42961 actactccca tattgtctga agtcgtcagg gtattggata ttcccttacc cactcgccct
    43021 ctccgtttgg ttgagtctgg atatgccaga ttgacagagt ctccaagagt tgcctttgcg
    43081 taccctcttt ttgtggcttc cttgattttc agaaatggca actcctctct cagcaaaatc
    43141 tttggcacct tatctccacc ctgcatggtg gtcaaggtcg gtgacaaacc agctatgtca
    43201 aatactctac cggtctggtc aaagctagtc ggcagatttc ctgcgacgac aacaccatgt
    43261 ctatcttgac tagtcaaagt aaacataggg tcttgattat ccttaaagcg acgcccatgt
    43321 tggcgttttt caagtctatc tggtgttaag actggaatgg cgatttttgc cccctctcct
    43381 ttacctctcg ttagtgtagg agcaagtcca gtcgtcagat agacttcacc attcaaacca
    43441 cgtttagagg gattgatatt tcctagcctt tcaagatgag ctgggctgtt ttctcttctg
    43501 agaggaaata agaatctgga acggtatctt ctagaatgtc cgataataaa gacccgttct
    43561 ctgttttgcg ggacttggaa gtccttactg ttaagcacct gccattcgac atcatacccc
    43621 aattcatcca gcgtggagag gattgtggcg aacgtccgtc cctcgtcgtg attgagtagg
    43681 cctttgacgt tttccaaaaa tagaaaacgt ggttggattt gtttggcgca tctaacaatt
    43741 tcaaaaaaga gagtccctcg agtatcttca aatcccaatc gtcttcctgc gagtgaaaaa
    43801 gcttggcaag gaaatccccc gcagataaca tccacttgcc ctctaaattg tctaatgtca
    43861 tggtctgtga cctctttaat gtcatggtat tctatttctt cttctgtctt aaacatggct
    43921 ttgtaggaag ttctagcgaa tttatcaatt tcacaaaagc ccaagcactc atggccctgt
    43981 gcttccattc ccagcctaaa accgcctatc ccagcaaata aatctaaaaa tttcattttt
    44041 cctctctttc caaataaaaa agagccttac acttgtaaaa ctcttgccta tatatgatca
    44101 tccatttctg gtgcacggat gatttcaacg atcaaaatga tgagcgaaat cagatagatc
    44161 ccacctagta tccaccaaat catcttaatc acctcacctt acttcttgtc gtctcagttg
    44221 cttggtccac tctgacagca caccattaaa cacgtattca gctatcactt ccgctgaccg
    44281 agcaaatctc atatgttcca atgcgtactg ataacgttca ctaaaataga tcatggcata
    44341 gtcactagcc gactgtgata gacctgtctg tcttaactgg tcatgaacca aatcccagat
    44401 atagtctcga tcgtactttg tcacggcatc gacttttgaa gaaaagtttt cttcttgttt
    44461 gtcatctact agctgcctct cctcctcgtc ctcaataagg aaatcactcc cttcagtttc
    44521 actatattca gtctcatttc cttcagtctc actaggggct gaatttaaga ctggccccgt
    44581 ctttttttga gcccccccct agtgtttttt taacactagc cccgtctgaa tctaaaactg
    44641 ggatagattc atgttctaat tcccccaagt aaattttatt tgccattcga cctttttcac
    44701 tagaggactg ttggacttca tcaattaagc catattcacg taaggttttc ttgatagaga
    44761 gtaattttga ctttgaacag cctaaaagtg ccatcagatt tgaattggaa taaatcaagt
    44821 aaattgcccc atcttcatca atccaacctt tactcaaaga caactccaaa cgatctttta
    44881 aaaccgcgta ggctacctta acctccagtt tcatatcctt ataccgttca ctctcaaaca
    44941 aaattttagg gagtttataa taacgctctg atgtctgata ttgatttgcg gttatacgtt
    45001 tcatgattgt ccctccaaga ctaatattcc aacatttcca aattcatcaa attggattaa
    45061 tcctacttgt tccatttcat caatgagctg agttgctttt acaatatcaa ctcccatgat
    45121 agccatgagc tgacacatca cgagttgacg tgatgattgt tcatactttt gcatcatttc
    45181 ctcctgaaaa taaaaaaagc gagaacactt ataaaaatgt tctcgctgaa ccaatatatt
    45241 ttctgacgta tctatttttt gtctacgcag tcgacatcca aacaaacgtt cacatcaatg
    45301 tacttttttt gaaccttttt tatgtcaaaa tcactctttt caataaagac aaaatgcttg
    45361 aaaaagtgct taaaataaag gatttttaca cgtttcttcg ttgtagcaat actacgcact
    45421 ccacgtgatg cgtttgagga aatagatcca ccggctggac tttcttcaat tcatatccca
    45481 attcttggta gagtttgata tcacgcgcca tggttgcgac attgcaggag atataggcga
    45541 tgcgatcggc tcctgtttgg gcgcttgctt tgataaagct ttctgtcaag cccttgcgtg
    45601 gaggatcaac caagataacg gttggttgaa tcccatcttt aagccaattc ttcatagcat
    45661 tttcagctgt gtcacagaca tagtgggcgt ttgaaatatt gttcagctga gcattttttt
    45721 tactattctc aaccgcttct ggaatcagtt caacaccgta gacttctttg acatgcttgg
    45781 cgactgataa accaatggtt ccaataccag aataggcatc aataatcacg tgatcttttt
    45841 ttaactctgc aaagtcaatg gctgtttgat agagtttctc cgccatttca gtattgactt
    45901 ggtaaaaggc tgggccagcg atttggaagt catttcccaa catctggtcc gtaatatagt
    45961 cttgaccata aagagtgcgc cactccttac caaaaatcgc attggtattc tggtcgttga
    46021 tattttgcat gacagacaca atctctggga actgcttgat aacttgttca atcaattggt
    46081 caacacgaaa aacttttgga cgagttgtca ccaaaacgac catgatttgt cctgaatagt
    46141 gaccacgacg caccacaaga ttccgaatca atccagactg ttccttttcg tcataaggtt
    46201 ttaaatcaaa acgacggagc aggtctcgaa gagctactac gacttggtca atgacaggat
    46261 cctggataaa gaaatcttca aggggcatga ggttgtgcga attcttacgg aaaaatcctg
    46321 tttccaagac accattcact cgacgaacgg gcacctgcgc cttattgcga tacttgactg
    46381 gatgttccat accaagcgtt tcagcaactt ctacatctgc aattccagca atcttgtaga
    46441 gactgtcctt gacttgcttg gttttaaact tgagctgttc tggataagaa aggtgtccta
    46501 aatccgcgat tcctgaacgc aggtaagcca aatctagatc ttgattacgg tgtggtgact
    46561 ggacaaggta tttttcaact tttccaaagc caatcttttt attgaccttg aggacacgca
    46621 tgagaatttt ttcactcggt aaagcattct ctacaaaaaa gaccaaacca tctaccttgg
    46681 caactcctgc cccttcatgg gtcaaatcaa caatttcaac ttctacaata tcatttttct
    46741 ttaacattct tttctctttc tattggccga caaaaaagac ggcaacgtta cggttaccat
    46801 ccattatacc atgaaatttc ttaagaacca aaactcttga agaagttgac aagggcttgc
    46861 cagagggagc ttaagaagtt accaccgtcc tctagcgcct tatccgcatc aaagttaagg
    46921 ttgatatttt taaaactgtc gccagcttgt gatacgatgc tttgtttaag gtcatttaag
    46981 gttttagtga aatctgcatt gctgaggata tcactctttg agagattcaa ggcaaaattg
    47041 atgatgatat tgatctggtt tcctgttatg acctgatcaa gtttgtaatt ttttaaggta
    47101 tcttcaacaa tcttgcggat atcttcttct gtcagatttc ccttgctttc tttagctttg
    47161 gcgagtcctg acttgatatc agctagggca acgtttaatt tattagcatc atagcctgat
    47221 ttgtccttgt tttcagcatt gatatctgac aaagccttta gctcttcttg agccaaatct
    47281 ttattggctt gcggcacctt ggctccatta gcttctagcg aatagtagat cccagccaaa
    47341 gcactctcac ctgtaactgg aatgggtgct gctacagtga ttttggcgtg ttccacaccc
    47401 aaagttacgg ctgcatttcg gtacatatcc tgagttacct tagtgatgtt ttcaggtgtt
    47461 tcaatcttga cctcaagtgg cgatttgtca cctagctttt gaatcttggc tgatgaatac
    47521 aactgtaagc tagagtcatt ggccacattc atgattttag aataaacatc aggtgtcatg
    47581 gtcttgagtt ctttggtatc tgttgaggca ttgtagccca gttttttaag agtttgattt
    47641 ttttggtctt cagataggga agaacctagg acatattcag gttggacata ggtttcatcg
    47701 ataacttttt gaacatctgt tgctgcatgg acgctattca tagctgttac tgcccacaag
    47761 atcgcagcgc tagtcagaaa gagtttcttt ctcataggga atttcctcct ttacttcttt
    47821 agagtaatat atctatctta aagaaaactt ataacaaaaa cacctggtct agccagatgt
    47881 tgaaaagaga gtgaaacatt tgatgatgta aaggttaagt cgtacctgtc tagaataata
    47941 atagtttcct ccatttacat agagttcagc accgtgaaaa atggaaatgg ggtgaatata
    48001 actataagtc tttccagtcc tattaccaag caagggggca acagtctcac gagagtactg
    48061 tttggctaga gccaaggtat tttccttgcc attttgggcg ataaaatcga tataggcagg
    48121 tccaaaatta taggcttgaa cagctgtcca gatatctaca cccttcttct gcgccagata
    48181 gagattgcct gtcagagttt gaatgccttg ccgaatgcta gaggcattat cattgatggt
    48241 gttggtggaa ccacttgcag actcactaga ctgcataaca tcgccttctt ttccttttgt
    48301 ttcagtataa atcatagcaa gcacaagctc ttcgtttgct ggggtgtctt tttcactcaa
    48361 tatttctcgc accatgggtt gataggtcat gacttgtttg acatcttgat gaacgcggta
    48421 agctttatag ccagcaaaaa ggaagactgc tagtacaagc actcttcgaa ttcgtttaaa
    48481 cattatttac tttggatatc ctcgatattt ttgattaaga tagagtaggt tccattttcg
    48541 ttttggataa actcaacaga ctcggcgtct tgatagacgt tattgggaac gatgagctca
    48601 attccatttg ataaggagag tttttggttt tcaaatttct ttaattggcg actggcatca
    48661 atttcatcaa attgaacagg ttctggtacg gcttctttga cttggtcaat aaagctcaaa
    48721 cgagccgtca gattgttgtc aaaaaggtca ttagccaatt tctcaggtga caattcattg
    48781 ctttcttcta ggttgttgaa aatagctgat ttgaccttgg attgaaattg aaaatcatct
    48841 gtgttaaaag attcagcaat tctctgggct gttttttcca gttccttgat agatttttta
    48901 ggagaaatct taggagcgac agcaagaaga ttatctgaaa aatagttcaa aaaagtcccg
    48961 ttgtacttga ttcgtttttc aatcaggtga tacttgctac tctgaagatt gaccaccaag
    49021 gcctcatcag ctcctgttcc aaatccaggc aggttattct gagttagctt gattggatta
    49081 tcaacttctc ctccgaggtg ggtcaaggtc tcccgcaggg caattcgcaa gaaagcgaaa
    49141 tgttctacac cttctttaga aaattgaaca aaaatcaagt cattggtctt gagattttca
    49201 gaaatgctaa actcctcttt ccagagatta gccagcgtta ctgatgtctc caacaaatcg
    49261 tctgtaatat gattgaagaa gggattttct tcttcgaaaa tcccagtctt ggcttcatct
    49321 gaatacacat gttcaatttt tttacgtagg tattcttcga tttttggagt aatattgaga
    49381 aacttatctg ctaagaacag ctcggtatca tccggactga actggtgaat aatggctttc
    49441 ttaatataaa tgtccataaa agttttattc ctcgtataat gggaaggcat ctgtcaattc
    49501 tttgactgca cttctcactt cttctaatac agcctcattt tctgaattct taagggtttt
    49561 aatgatgagt tcagccactt tgcgactttc ttcttcacca aatccacgtg cagtaatagc
    49621 tgctgctccg atacgaatcc cacttgtctt gaatggtgac aagctttcgt aagggattga
    49681 gtttttattt aaggtaatat tgacttcatc caacaagttt tgagcaactt tgccgttttc
    49741 tacaacttta gtcacatcaa caaggaagag atggttttca gttccacctg aaataatacg
    49801 gaaatcaggg tcttgcaaga agacatctgc catagccttg ctgttcttaa ttacattggc
    49861 agcatattcc ttgaaggctg gatccaaaac ttctttgaag gaaactgcct tagccgccac
    49921 aacatgctct aaaggaccgc cctgaatacc tgggaaaata gctgaattga tttttttagc
    49981 aagttcttcg tcattggtca aaatcaaacc accacgaggt ccacgaaggg ttttgtgggt
    50041 cgttgttgtt gtgatatgag cgtatggaac tgggcttgga tgaaggccag ccgcaaccaa
    50101 accagcgata tgggccatgt ccaccatgag cttcgcaccg acagcatctg cgatttcacg
    50161 gaattttgaa aaatcgataa tttgagaata ggctgaagca ccagctacaa tcagttttgg
    50221 ttttacttct tgggcttgtt tcaagatagc atcaaagtct aagagttccg tttcaggatc
    50281 aacactataa gaaacaaagt tgtaggtttg accagagaag ctaacaggag ccccatgagt
    50341 caaatgacca cctgatgcca aatccattcc cataaccgta tcacctggct caatcaagga
    50401 catgtaagcc gcacagttgg cctgacttcc tgaatgtggt tggacattgg caaatttagc
    50461 accgaaaatt tcttttgcgc gttcaatagc aagagtctct acaacgtcta ctacatcagt
    50521 tccaccataa taacggcgtc ctgggtaacc ctcggcatat ttatttgtca agatagaccc
    50581 ttgagctgcc ataacagcct tggaaactac gttttccgaa gcaattaact cgatattatt
    50641 ttgttggcgt tcttcttctt tggcaatagc attccagaga tcagcatcat atgctttaaa
    50701 atcatctttg tcaaaaatca taggtcttct cctttattgt gtgactagtc cattagtttg
    50761 attttacaat aagaaaatca aactaaaaga tgcgaataaa ccgtttctgc attttatcac
    50821 aagtatagcc aactttttca taaaatgcat gagcacccag acgatgattg gcagaattta
    50881 agcggataaa cccataacca catcttttgg cttcttgttc caacccttgt agtaaacttt
    50941 taccgatacc ttgaccttgc gcttgaggtg aaactgctaa agctaagata ttaaatcctg
    51001 ctttggaata gagtgattcg taaacttcag cgtggacata tccaagtaag acatgattag
    51061 ctgcatcctc atagccaagt aggaaatgat gggaatcctg agacagtcta gctagttggc
    51121 tagccgtttc ctctggacta aaagtataac ccaaagcctc ttggttgatg tcacatatcg
    51181 ctttcacatc tgtttcttgc aaatctctta gcatttcaag cctcctcaaa agaaatctct
    51241 ggcaaccgag caagaatatc ttctcgttta attgcgcctt ggcgtaagat tttcaccttg
    51301 tctccagaca aatccacaat agttgaatcc tgtccagtta gaaaagcatc gtcttccaga
    51361 cccagaacct cttggtcaaa atccttcaga atttgttcaa aggttacacc acttgcctga
    51421 cctgagatat tggcagacgg cccaatcaag ggacctgtct ctcgaattaa atccagtgtg
    51481 atagggtgac tgggcatccg aaatccaata gttgcaaggt cagaatttac ccaataggga
    51541 actcggtcat tggcttcgag aataatggtc aagggacctg gcaaaaaggt ctctacaagt
    51601 ttttgtagat aagctggctg attctttgaa aagtgcaaga tgtcctcgaa agaggcgata
    51661 ttgagattga gtgccttatc tctaggacga cgtttgagtt ggtaaacatg gtcgacagct
    51721 ttttcatcta aggccttagc aaaaagaccg taaaccgtct ctgtaggtag aacgacagct
    51781 ccaccatttt ccaactcttg tctaatcctg tccatcatca accacaacca tcctattttg
    51841 accaaattgg tccttgagtg ttcgaactct tttttcagga agatgtttcc taaaaagttc
    51901 gggaacactt tgaccttgct tgtatccaat ttcaaggtaa atcttaccac catctttgag
    51961 atagtctttt gcatcttccg caattctaca gtaaatagct aggccatcct cgtctgcaaa
    52021 gagagctaga tgaggctctg aatgcaaaac attcaagcct acctctgact catcttcacg
    52081 agagatatag ggtggattgg atacaattat atcatatttt tcagaaattt ctgcaaaaca
    52141 atcagatttt ttaaaaaata tattaagatt ttgattgcta gcattctctg atgcaagctc
    52201 taaggcctct tgagaaacat ctgctgctgt cactgaccaa tctggtctat tttttgctag
    52261 agcgagagca atagctccac tacctgttcc aatatccaga actgaaagat tcgtctcaag
    52321 attttcagtt agaataagct ccactaactc ctctgtttct ggacgaggaa tcaaaacccg
    52381 ttcatccacc tttaaatgca ttccataaaa atctgcctgt ccaatgatgt actgagctgg
    52441 cttgtgagct gctagttgct ggtaaatatc ttctacaaat tgtttttctt cctctgttgt
    52501 cacctcctgc tggagggcaa aaataaagtc tgtaaaagat agatttttca gactacgata
    52561 gacaaaagag aggctttccg cttcctctcc ttgtcttatc aactcttctt caaaatttga
    52621 aaataattga gctaatttca ttatttgttt aattcttcta gtttttgtgt ttggtcataa
    52681 agcaccaagg catccacaac ttcgtccaat ttaccagaca aaatcgtatc tagtttttgg
    52741 agggtcaagc cgatacggtg gtctgtgaca cggttttgag ggaagttata agttcggatc
    52801 cgttctgaac ggtcaccagt accgattgtc gacttacgct cagcgtcttg ttcatcctga
    52861 gcaatctgag caaagtggtc agcgacgcgt gcacggataa tcttcatagc cttctcacgg
    52921 ttcttctgct gggtacgttc ttcctgcatc tcaaccttga tattggttgg caagtgaacg
    52981 atacgaacgg cagtcgcaac cttattgacg ttctgtccac cagcacctga tgcgtggtaa
    53041 atatcaacac gaagatcttt tggatcgata tcgtattcta cttcttcgac ttcaggcatg
    53101 acaagaactg tggcagttga ggtatgaaca cgaccttgac tttctgtcac ggggacacgc
    53161 tgcacacggt gggcacctga ttcatactta agcttagagt atacagactg acctgaaacc
    53221 atagcaacca cttctttaaa cccaccgaca ccattcatag aggcttccat gacttcaaag
    53281 cgccagcctt gggcttccgc atacttttgg tacatagtta gcaaatctcc agcgaaaagt
    53341 gccgcttcgt ctccaccagc tgctccacgg atttcaagga tgatattctt gtcatcgttt
    53401 ggatcctttg gaaggagcaa aattttcagt ttttcttcat attcttcttt ttcagccttg
    53461 gcatctttga gttcttgctt ggccatttct tccaagtccg catctccgcc tgattcctta
    53521 atcatctctt cggcatcgac gatattttga aggacttgtt tatactcacg gtaggctatt
    53581 acggtatcac gattggaagc ttcttctttt gaaagctcca taaaacgctt ggtgtctgaa
    53641 acgacatcag ggtcactcag caattctcct agttcttcat aacggtcttc tacagcttgt
    53701 agttgatcat agatgttcat ttttcttcat tctccttatt gatttcaggg gtaaaataat
    53761 gtttacggca aaccgagata taggtttcat taccaccaat ctgaatctgc tcaccatcat
    53821 agaccggcag tccatcctgt gttcgcaaaa ccatggtcgc ctttttctta caatactggc
    53881 agatggtctt gatttcgtca atcttatctg ctaaaagcaa gagatacttg gaaccttcga
    53941 acagttcatt gcgaaagtca tttttcaaac caaaagccat gacaggtatg tctaactcat
    54001 caacaacacg agctaggtcg taaacatggt gacgtttgag aaactgagcc tcatcaacca
    54061 atacacagta agttttttct ggtaggtctc ggatatagcc aaagatatcc gtcgtctcct
    54121 caatcgcaag agcagggcgt ttcataccga ttcgactcga cacatagcca ataccgtcac
    54181 gcgtatccag agccgaggtc ataatgacaa caccttttcc ttgctcctca tagttatagg
    54241 ccaccttgag aatctcaatc gtcttaccag agttcatggt cccataacga taatacaact
    54301 gtgccatgtt tcttgcttca cgtccatttc taaatttttg ctacattcta gtatatcata
    54361 attttctgaa gctttaaacg gcaaaatgtg gtaaaataga agaaatcaaa aactagtgga
    54421 ggaagctatt atgccatttg tacgcatcga tttatttgaa ggacgcacgc tcgagcaaaa
    54481 gaaagctctt gctaaggaag taacggaagc tattgtccgt aatactggag ccccccaatc
    54541 tgctgtccat gtcatcatca acgacatgcc agaaggaact tacttcccac aaggggaaat
    54601 gcgtaccaaa taagctagct taagcagaat tgcttaggct ttttcaatct ccaagtaaca
    54661 tccattgaag aaatagccta aatttgttac aatttgaaag gatacttgga aaaatttcca
    54721 agaaaagagc tatttattaa aggaaacatt atgattacac gtgaatttga taccatcgct
    54781 gctatctcta ctccactagg tgaaggggct attggtattg tccgcctgag cggaacagac
    54841 agttttgcta ttgcgcaaaa gattttcaaa ggaaaagact tgagtcaggt tgctagccat
    54901 acacttaact acggtcacat cgttgaccct ctgactggta aagtcatgga cgaggtcatg
    54961 attggggcta tgaaatctcc aaaaaccttc actcgtgaaa atattatcga gattaacacc
    55021 cacggtggga ttgcggtgac taatgaaatt ctccagctag ctatccgtga aggagctcga
    55081 ttggctgaac ctggtgaatt tactaaacgt gccttcctaa atggacgtgt tgacttgact
    55141 caggctgagg cagtaatgga tatcatccgt gctaagacag ataaagccat gaacattgcg
    55201 gtcaaacaac tagatggttc tctttctgac ctcattaaca atacccgtca agaaatcctc
    55261 aatacacttg cccaagtcga agttaatatc gactatcctg agtatgatga tgttgaggaa
    55321 gcgactactg ctgttgtccg cgagaaaact atggaatttg aacaattgct aaccaatctc
    55381 cttaggacag cacgtcgtgg taaaatcctt cgtgaaggaa tttcaacggc tatcatcgga
    55441 cgtcccaacg ttgggaaatc tagcctcctc aacaatctct tgcgtgagga caaggctatc
    55501 gttacagata ttgctggtac cacacgtgat gtcatcgaag aatacgtcaa catcaacggt
    55561 gttcccctaa aattgattga cacagctggt attcgtgaaa cggatgatat cgttgaacaa
    55621 attggagttg agcgttcgaa aaaagctctt aaggaagctg acctagttct gctagtacta
    55681 aacgctagtg aaccactaac cgcccaagat cgccaactcc tagaaatcag tcaggagact
    55741 aatcgcatta ttcttcttaa caaaactgac ctgcctgaaa cgattgaaac ttcggaacta
    55801 cctgaagatg tcatccgcat ttcagttctt aaaaatcaaa acatcgataa aatcgaagag
    55861 agaatcaaca acctcttctt tgaaaatgct ggtttggttg agcaagatgc tacctacttg
    55921 tcaaacgccc gtcacatttc cttgattgag aaggccgttg aaagcctaca agctgttaac
    55981 caaggtcttg aactagggat gccagttgac ttgcttcaag ttgacttgac ccgtacttgg
    56041 gaaattctag gagaaatcac tggagatgct gctccagatg aactcatcac ccaactcttt
    56101 agccaattct gtttaggaaa ataagaaaaa tccatgatcc ttcattcggt catggatttt
    56161 attgtcttta ttagtaatct ggtcttaaga cccctgttac agttgcctta gttgcttcgt
    56221 agtcgccatc tacgacaacc ttgataatgc gtttggcatc ttcttctggt gctggaataa
    56281 gaggtagacg agtgggtcca gcttcaaatc ccatatagtt aagaactgcc ttaactggag
    56341 caggacttgg ataagagaag agagcattaa ccttaggaat gaatttacgc tgaattgctg
    56401 cggctttctt catatcgctt tctgcaatgg cagtaaacat ctcgtgcatt tcatccccat
    56461 ttgtatgaga ggcaacagaa ataaccccat ccgccccaag gttcatggca tggaaagcat
    56521 ctccatcctc acctgtataa atcaagaact cttcaggctt gtgctcaatc aagtaagcca
    56581 tattagccaa gctagtacat tctttgacac cgataatatt tggatggtca gccaaacgaa
    56641 gcatggtttc tggagtcaat tcgaccacta cacgccctgg aatgttatag ataataattg
    56701 gtaggtcaga agcatctgca atagccttaa agtgctgata catcccttct tgagaaggtt
    56761 tgttgtagta aggaacaata gcaagaccag cagcaaaacc accaaattcc gctacttctt
    56821 tgacaaactc aatagagtca cgcgtatcat tagtacctac acccgcaatc aaaggaacgc
    56881 gtccattgac aaccttttgt acagccgcaa acaactccaa ctcctcatcg tgggtcaaag
    56941 ttggactctc agcagtcgtt cctgcgagaa gaattccatc cgtatgatgg gccaataaat
    57001 gctcaatcaa ggctggaata gcatcaaagt taatggaacc atcctcatgg aagggggtaa
    57061 taaaggctgt aatgatttta cattctttta aatcttgata agacacgaaa acacctctct
    57121 atttcaaaga aggagttcat ctgaactcct aaacgatatg actattttaa ttcaaatttc
    57181 aattcggctg ttggacgaac caatccacgt tcatgaagag tttcagcaat ctgaactgag
    57241 ttccaagcag cacctttgag aaggttatct gaaacaaccc acatgtgaat tcctttttct
    57301 gcatccaagt ctttacggat acgaccaaca aaggtatcac gcgaaccaac tgcattgata
    57361 gcttgaggat agatttgatg agctacatca tcttcaagaa cagcacctgg gaaggctgcg
    57421 atagctgctt ttacttcttc gattggagcc acttcttttg tttcgatata aacagactca
    57481 gagtgagctg acaagactgg aatacgcaca catgttgcag atactgcaat gctatcatct
    57541 tccataattt tcttagtttc cttggtcatc ttcatctctt cgtacgtgta atcattatca
    57601 gtgaaaacat caatttgtgg aagagcgtta aaggcgatag gataatgttt cttgtcacca
    57661 cctgaaggca agatttccgc atgcaaatca cgtggtttca caccatcatt caaaacctca
    57721 cgaagttcac gttgtgtctc aagaattgct cccataccag cacctgaaac ggcttgataa
    57781 gttgaaacaa tgatacggtc caagccccat ttttggcgaa ccggctcaag agccaccatc
    57841 atttggattg ttgaacaatt agggcaggca atgattccgt tgtgagcatc aagtgcatga
    57901 gcattgacct ctggaacaac caaaggaaca tctggatttt gacggaaata agatgtatta
    57961 tctactacta ccacgccagc ttttactgcg tatggtgcat acttagctga tgtcgaacca
    58021 cctgctgaaa agagagcaat atcaactcct tcaaaagctg tttcagtcgt ttcttcaatt
    58081 gtaatatctt gatctttaaa tttcaatgac ttacctgctg aacgtgcaga agcaaggtaa
    58141 cgaattttat cgattggaag tgttgattct tccaacattt ttatcatctg agcaccgaca
    58201 gcacctgtcg cgccgactac agcaactgta tatcccataa ataacctctt tcggaatttt
    58261 ctaaaaattt ctataataga agtattatac tactttttcc atgaattgca aagctttttc
    58321 atcatttttt ggaaaataaa aatcccagtc gctagactag ggactaagag gcatcttcat
    58381 gtgatgagca ggttcacaca actcatcaag gtccgctcct gcgttatgac ctccttatgc
    58441 tcaatagtag tccgaagact acctatcgac tcatcgcata ttctatatta ctagagatac
    58501 ttgattttgt caataagaaa ttttttcgat cgagtataac aaaaaactct cgtattcaag
    58561 agtttcccta tacatatcat gctaattgcc gggattgaac cggcgacctc atccttacca
    58621 tggatgcgct ctgccaactg agctaaatca gcttacttaa gaagtatact atattcagaa
    58681 aaacttgtca agtttcctta gaaattttcc ataggaaaaa gccaggtaag aactacctag
    58741 cttatcttct tctatttact taaatcaatt cgacgaccaa ttttaccaat aataatcgcc
    58801 ccaataatca atgaagcaaa ttcaccgatt cctgttgtaa accaagtaag gaagaatggt
    58861 aattctgcta caatatttaa ctctgcagcg atagtaaaca tggaaattga aaagaggatt
    58921 gaaaagaaga aatgatcttt tcgaatcaat ccgttaaaga ggtaatcctt gctatatttt
    58981 gcaaaaagcc aaacacctaa actgaggaat actaaggttg aaccaccacc aacaaagaca
    59041 tctagcagtc cgaagctaaa gaaattagca atcatacaac cgatggtaac tccgataatg
    59101 tacttaggat tgtaaaaagc cataaagttc atcatttctg aaatacgaaa ctgataagca
    59161 ccatagctga tggcatttag gggcggtgtg acagtcaaaa cgacgtagat agcagcgacg
    59221 attgcaatat ctgcaacatc acgaatagtt aattttttca tcttttctcc tttggcggtt
    59281 gcccgcgtgt aatatgcttg gtgaaagaag ctaagcacca agggttgctt tattcaaccc
    59341 tactagtata gcacaatagc ctgctttatg ctatacttaa cacatgaaaa ttcctattca
    59401 aaaatttttt aacaatgaaa tcttagccta tctctttttt ggccttgcga caactctagt
    59461 ttcgattctc tcacgactag tcatttatca actaagtcat caggaactcc tggctactgc
    59521 cctagcaaat attatcggta tcctatttgc ctttatcaca aatgatacga ttgtatttaa
    59581 gcaaaaaaag aaaaatagac ttatccgttt agtgaaattt tctcttgctc gcctttctac
    59641 ttttctgtta gacttgtttt taacttttct ctttgtaacc caatttcctc acattatagg
    59701 gcaattcgta actgaaaaca ttgataagat aaatgctatt gaaacaataa ttgcacaaat
    59761 attcattatt atcatcaatt atatcttgag caaggcctat atttttagaa aataatgctc
    59821 catttatgag ccttttaatt gccttctata atgatttagg atacaatgaa acacgaaaca
    59881 ttgaaaggaa aacagtatgt taaaaaatct aaaatcgttc ttgcttcgag gaaatgttat
    59941 tgaccttgct gtcggtgttg tcattgcctc tgcttttggt gctatcgtta cttcacttgt
    60001 aaacgacatt atcactcctc ttattttaaa tcccgctttg aaagctgcta aagtcgaacg
    60061 tatcgctcaa ctttcttggc atggagtcgg ctatggtaac tttttaagtg ctattatcaa
    60121 ttttatcttt gtgggtaccg ccctcttctt tattatcaag ggcattgaaa aagcacagaa
    60181 actgactggc ataaagaaag aaaaaactgc cgaaaaaaaa ccaaccgaat tggaagtcct
    60241 tcaagaaata aaagctctcc ttgagaaaaa ataatcgatt aaaaggatct agcgcaaagc
    60301 taaatccttt tttctttact ctttgggtaa cttgcctgct ttttcaagca tttttttgat
    60361 cagataaggc atcttaatat tctcccgccc ttttttctca atcagttttt tcacaaattc
    60421 tggcatttgt aaatcttcag attcagctaa aatgtttttc gtctcgtctg aaggaactaa
    60481 ctcatcaaag gtctcttctt ctgggagaaa ttccttaaac tcttgatttt cattgactct
    60541 aactgtattg accaaagcat caatcaaacg agaatctgta tttggcattg gtggacgatg
    60601 gtagtttacc ccaaattcct ggcataagtc ataacattct acatcattgt caaacaagac
    60661 ctcaatatgc tcactgatga aactaatagg tacaaaaata taatggtctg gatgttctgt
    60721 ctgttctctg agatactcca aaacatctgg tttaatccat ggaataccaa tatcactctc
    60781 actctgccaa gtgttggtat attgttctga actcaagccc agtttttcag cgactagctt
    60841 gctattttcg aaaatttggt ctatataagg atcaccaaag tccaaggcaa aaatgggcac
    60901 actatgggct gaaaagatga ctttaaagct gtcttgcttt acttcttctt ttaaaatctt
    60961 agcaatttca tctgcccaat agtttaagag cgcttcttcc tgataccagt ccttaatgac
    61021 caaaaattga atttgtttgc tttctaaaaa tttttcatag cccatgacag agtagaaaga
    61081 ataatgaggc tccaaaatca agcaaataca ctgttcaatt ccgtctgctt ccatttgacc
    61141 gatcacatct ggaataaagg gactggaaaa cttattagca aagtagacac tgtattcatt
    61201 ccccagccta gcttctacca aggcaacctc ttgacgggta attttttgca gaggcgtgcc
    61261 tccaattcgt acataattat catagagagt ctgaatctcg tggtcttggg gtctcacccc
    61321 acgacgaata tttgtgaaaa aatcagctac accttcaaag gtaatctctt ctggtgaacc
    61381 gaaggtcatc attaaaattg cttttttcat gactgcttcc ttgtgatgtt tttatcaagt
    61441 ttattttatc attaattcat cgataagtaa acgctagcat agggacattc tcaatcttct
    61501 gtcttttgtt tttctcttct ttctatgata caatggaaaa aataaattca aaaggagttt
    61561 ttttatgact tatccaaatc tcttggaccg cttcttaacc tacgttaagg tcaacacgcg
    61621 ctctgatgaa cactctacta ctactccaag tacacagagt caggttgact tcgcaacaaa
    61681 tgtcctaatt cctgaaatga aacgtgttgg actgcaaaat gtttactatc taccgaatgg
    61741 ttttgctatt ggaaccttgc cagccaacga tccgtcttta acacgtaaga ttggttttat
    61801 atcgcacatg gatactgctg attttaatgc tgaaggagtc aatccacagc taattgaaaa
    61861 ctacgatggt ggtgtgattg aactagggaa ttctggtttc aaactcgatc cagctgactt
    61921 caagagtctt gaaaaatatc caggacaaac gctcatcaca acagatggaa caaccttgct
    61981 aggtgctgat gacaagtcag gaattgctga aattatgaca gccattgaat atctaactgc
    62041 tcatcctgaa attaagcact gtgagattcg tgttggtttt ggtccagatg aagaaatcgg
    62101 tgttggtgcc aataaatttg atgcagaaga ttttgatgtg gattttgcct acactgttga
    62161 tggtggtcca ctaggtgaac ttcagtacga gactttctca gccgctggtg ctgaattgca
    62221 tttccaaggc cgtaatgtcc accctggtac tgccaaaggg cagatggtca atgcccttca
    62281 gctagcaatt gattttcata atcaacttcc agaaaatgac cgacctgagt taactgaagg
    62341 ttaccaaggt ttttaccatc taatggatgt gacaggtagt gttgaggagg cgcgtgcaag
    62401 ctacatcatt cgtgattttg aaaaagatgc ctttgaagcg cgtaaagcat ccatgcaatc
    62461 tatcgctgat aagatgaatg aagaacttgg gagcgaccgt gtcactctca acttgacaga
    62521 ccagtactac aatatgaaag aagtcattga aaaagatatg actccaatta ccattgctaa
    62581 agccgttatg gaaaatctag gtatcacgcc tattatcgaa ccaatccggg gtggaacaga
    62641 cggctctaag atttccttta tgggaatccc aactccgaat atctttgcag gtggcgaaaa
    62701 tatgcacgga cgttttgaat acgttagcct tcagactatg gaacgtgcag ttgataccat
    62761 cattggcatc gtagcttata aaggctaaaa agacgaggtg gctcagctac ttcgcctttc
    62821 tttttattct actggttttt cttgatttcc agtagttgta gaagattctg ttgtttcatt
    62881 ttctgaagtt gattcagcag gtttagaatc tcttgtattg cttggtttgt tttcgtcgct
    62941 agcagtttca atgttagatt ctgcagttgc gtttggttgg ttctcagcac tggtgttatc
    63001 accatttgct tcagcatttc ttgctggact tgtttcttca cttgcgctag cttttgactg
    63061 gatttgatga ttcaaaacta gaatagcttt tgtcggttca agtaaagctg ttttgtcttt
    63121 actcttagca gaaagttgat ctaataatgc atccacctta tcaaagtccg catcagatcc
    63181 attattactt tctaaataag agtgaagcga catgagaata tcgtagagtt tttgatagag
    63241 tacaagtgtc tgaggatctt gctcagcatt ttccttttct tgttgaaggg cgctagcgat
    63301 acgagtcaaa acatctttta cctgactgtt tacttcatcc aagtctgcat cagccttgtt
    63361 tgtggcagct tttagatttt ctacttcttc tgccaaggat tgtctgattc cttcttcatg
    63421 gatttgttcc aagagttgat ttgccttgct caaaagactt tctacttctt ccttgctatc
    63481 tgtcgcagat tattggttgc catctaccat gtactcctaa aacaggagag ttataatcca
    63541 agattacaag gccttacaga aataagaaat ccagataaga caatgttcgt ccaagacgct
    63601 attcgcttcg cacagcagca cggattcaat atgctttaat tttaaagttt aggtgtcaag
    63661 acctcttttt agtgtgccca aaatttagag aagtaatcaa tcaactaact tttatttttt
    63721 tcaaactttc agtaaactga cctaaagcta actcaatctg tctttgttcg ataggcttgt
    63781 ctttgtagat gcttctgcta tcagctagaa gttgatctac ttttgccaag actgccttct
    63841 catcaaaagt tccaggttga tagttggatt gcagggatgg aatcttgttt ttcaaagccg
    63901 cttcatatcc cttagtttga accttgatgt agtgattgtg gtcgccacga ggaatcacaa
    63961 aaccttctga atcttcactt ataattcgat tggcatcaaa accatgacca tcttcttcct
    64021 catggtggac atgtagtgac ggattactta atacagaact agaagaactt cctacctttt
    64081 ccgtgttaga gtgtgatggg ggattgttaa gagatgactt aggaatatag tgatagtgac
    64141 cccatgtctt actatataag catcacctgt atctctgaca atatcattag ggttaaagac
    64201 atatgtggct gctaattcac ctgccgacaa gtcactctca ggaatgaaat gatagtgacc
    64261 accatgtggt actatagtag gttgaaatag aatatgagca aatttataag gggattttaa
    64321 agtaatttct aacaatgatt tagaaactat gatgtgctat tctaaattca actcactata
    64381 tataaccatc atcggtagta taacgtccct gtaattttgc tacagatact tctgcactag
    64441 ctcctttatc gtctttacca tgttcttgtt tttggcgatt gatttcatct tttgttcgta
    64501 cattttctgc atgagcttga tctttaaggt aaacataata ctttccatct accttaataa
    64561 tatatcctcc cttaacctaa ctgacgatat cttgatcttt cggctgatag ttgggggctt
    64621 tcattagtag ctcttcacta aagagcgcat caaaaggaac tttaccatta tagtagtgat
    64681 aatgatcgcc atgagaagtt acataacctt gatctgtaat cttaataaca atttgttttg
    64741 cttgaattcc ttctttttga ctaacctagt ctggagtcaa attttcagtc ttcttagtgt
    64801 ctttattact gtttacatat gaaacacgat ttttatctgt attggcctgt tagctatgtt
    64861 ggttcagagc ataaacacac agacttaagg aaaggataac aacagatcca gctgctatat
    64921 atttcttttt aaatttcata attacctcat ttctataatt atttatatga tgtcttcatt
    64981 attaaatgat taaataaatt aattaaccag ttaattaact agtaaatatt ccacctcttt
    65041 ttaagttgta tgtcaagaaa ttttatatat taataataaa atgaaattct cccaaagtca
    65101 gagttttatt tctaactttt gagagaactt catttttgat tcagactttt tctactgcta
    65161 ttccttacgc tatgagatca gataaattct tttttatcac ttctccactt ggcaatctta
    65221 attcaatcgt tccatccata ttgaatataa cactatctaa gcctaatccg taactagctg
    65281 taaatttttc taatttttct tgtacaggat ctactgctgg agcttcctct aatgctggat
    65341 ctaacatagg gtcactcccc acattccctt ctgaattcaa cattccatta tccgttgagt
    65401 tttctggttt tacaggtttt tcgtttggtg cctctggtaa agaatctgct ggtttatttt
    65461 ctgttggttg gttctcaact gttccagtag atacttttcc attttcagat ggtttatttt
    65521 caccatttcc ttgaggtgct tctcctgtaa aatctgccat attcttttta atgacttctc
    65581 ccgatggtaa atataattca attgttccgt ccatattaaa caagacattt tctagcttca
    65641 tcccataact ttcagcaaat tttgctactt tttcttgtac aggatccact gtaggaactt
    65701 cttctaacgt tgaattacta gtactattcc cagtttcaga aagtttttct ttttctacct
    65761 tctcactagt ctttggttct tctacctttt catcaagttt tgagttttct tgtgctttat
    65821 tccttttaaa ttgtggtaga atacttggtt tatcagtttg attttctttt tccaagatag
    65881 gtacttccac aatataagtc gattgattgt ccaaataagc atttgccatg aaggttacag
    65941 gaattttatt tccggccgtt ctggttgttc cttggtttaa tttcggaatc gataatttga
    66001 tttcaccaac tttatagtta ttttctaaat aagcatttcc atgaaattca tcaaacactc
    66061 tgactaaagc atcagttcct ttaggcactg caaattgagg gttcactctt aaataagtat
    66121 cccctgcatg gaaaggatag aaaatcgttt gactggccat tttgtaagct aaagaggttg
    66181 gaactgtaaa tgtaccatca taacttactt ctggataatc ttttgaagcg atagtatact
    66241 taaatgtttg tcctggtaaa taaggttgat ctaattcaaa gtttgcaata ttccctactc
    66301 cttctccaaa tactttacca gatactttct ccaatacttt tccatctggt gttattaatt
    66361 ttactagcat attgatacct aattttttct ccaattcagg cggaaaacta aaagaaacgc
    66421 gtttttgacc attggctaga gtaaagtttt gattattaaa cgtactattt tttaacaaat
    66481 taacaacatt cgttaattct tctccagtat aaactttatt cccttctttt ttagcaactc
    66541 cttcttcggg tttaaacagt tcatagttac tgtgagaatg accaattcca accggtttat
    66601 gttcatcaat cggatctgca tgatggtgat ctccatgcgg ataaataatc gcattttttt
    66661 ctttattcac gacaatactt tcacgtttga caccatattg tttcataatg ccagcaattt
    66721 tttcttcgat ttttttatct aaatctttca tttctttggc attacttgga taatcctgtt
    66781 catgagatga caaagaatct aatccattat gactagtttt aacttcctct aaatgttttt
    66841 gcgcagcctt aatttgctct tctgtcaagt ccttcttgaa gaaataatga ttgtggtctc
    66901 cgtgactcat gacaaaacct gattcatctt cagcgataat acgattagca tcaaatccgt
    66961 atccatcttc ttcatgtttc tcatgtgaag ttcctggatt gattggaaga gatggagaag
    67021 gtgttgctag actattgttt ggaagagtcg gttgcccaat ttgatttgat tttggaatgt
    67081 aatggaaatg atcaccatgt cttacaatat aagctgtagc cgtttcttca acgatatctt
    67141 ttggattaaa aatataacca tcagatgctg aagagagctc cttacttgtc gttaaagaag
    67201 aaggattgct tgaaagactg cctagactag acactacttc attaggtttt gcatttgtag
    67261 aaactgtaga accagttcca ctgataggca ccattctggc aatcttttct tctaaggcag
    67321 aaagcttgct gtaaggaata aagtggtaat ggtcgccatg cggaatcgca actccatttg
    67381 gtgtacgact gataatctta gcagggtcaa agaccaggcc atctgattca ctgtaacgtt
    67441 gggcgctagg tgaatcatag agttccttca aaagactctg gagattttca gatttatttg
    67501 ctggcttgct aattgatcct tttgctacag attgcgtgtt attgtcacta gctgttgaag
    67561 aatagcttaa ctgactcggt tgcatatttt ttccagccag atgtgcttta gctgctgcta
    67621 attcactagc agataaatcg cttttgggaa tgtagtgata gtgacctcca tgaggaacga
    67681 tataagcatt acccgtatct tcgataatat cagctggatt aaagacataa ccatcatttg
    67741 tcgtatatcg tccctgagac cttgctacag caacattaga gttaaccttc tcattatctt
    67801 tgacatgttc ttgtttttga cgattgattt catctttagt tcgaacatta tcagcatgag
    67861 ctgcatcttt caggtagaca taatattttc catcgacctt gatgatataa ccacccttga
    67921 cttcattgac aatatcagcg tctttaagtt gatagtttgg atccttcatc aagagttctt
    67981 cactaaagag ggcatcataa ggaactttcc cattatagta atgatagtgg tcaccgtgtg
    68041 acgttacata gccctgatct gtaattttga ttacaatttg ctcagcctga attccttctt
    68101 tctggctaac ctggtctggt gtcaagtttt cacttttctg acttgactgg ctgccatcca
    68161 cataagagac acgattattg tccttatttt cctgcgaacg atgctggttt agtgcatagg
    68221 cacataaact caaggatacg ataacagctg atccagctgc tatatatttt ttactaaatt
    68281 tcataaatcc ctcatttcaa taaatgatga agttttttct caacttcttt tactttatta
    68341 aatagttttc taaaccggtt atttaaccat ttaattaacc agtaaatagt ttactattta
    68401 aaattacttt tgtcaagttt ctgttacgtt tttctaaaaa agagaagtaa cacgaaaggt
    68461 tacttctcca taaaatatgc tccattcatt ttactactgt ataggagtcg gttgactttc
    68521 ttttaacaaa gccaagagac tatctacttc tgctgaaata gtgttattat ctttcgttcc
    68581 gagaagaaga ctacttttta gaccagtcaa tgtctccaca gcattttgtc taatactaga
    68641 atctgttact ttttctagca aggcctccgc ctctgctatc ttagcgttaa taacagaatg
    68701 ctctacttga ggaatttcag cctcatctgt agtatcttca gcttcttcct ctgtctcttc
    68761 cgtatcagtg cttggtttat aaagattatc tgctgaagga tttaaaccag cgtgattctc
    68821 tttttcatca gattcagggt gagttggctc acttacttca tcatgttcct tatcttcctc
    68881 aggtttttct gtctgaggtt t
//
LOCUS       DAVDWM010000007        56219 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVDWM010000007 DAVDWM010000000
VERSION     DAVDWM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.007"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(32..262)
                     /locus_tag="VUW49_000555"
     CDS             complement(32..262)
                     /locus_tag="VUW49_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980517.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(280..921)
                     /locus_tag="VUW49_000556"
     CDS             complement(280..921)
                     /locus_tag="VUW49_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980518.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(933..1667)
                     /locus_tag="VUW49_000557"
     CDS             complement(933..1667)
                     /locus_tag="VUW49_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET7980519.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINFYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1670..1867)
                     /locus_tag="VUW49_000558"
     CDS             complement(1670..1867)
                     /locus_tag="VUW49_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980520.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(1867..2073)
                     /locus_tag="VUW49_000559"
     CDS             complement(1867..2073)
                     /locus_tag="VUW49_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7980521.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2083..2397)
                     /locus_tag="VUW49_000560"
     CDS             complement(2083..2397)
                     /locus_tag="VUW49_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980522.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(2550..2723)
                     /locus_tag="VUW49_000561"
     CDS             complement(2550..2723)
                     /locus_tag="VUW49_000561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980523.1"
                     /translation="MDRTDEVSSKHGFEVVDETDEVSSKYCFEVVDRTDEVSNHTYGK
                     VKLTWFEEIFEEY"
     gene            complement(2712..2792)
                     /locus_tag="VUW49_000562"
                     /pseudo
     CDS             complement(2712..2792)
                     /locus_tag="VUW49_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(2783..4072)
                     /locus_tag="VUW49_000563"
     CDS             complement(2783..4072)
                     /locus_tag="VUW49_000563"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET7980524.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKEA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVSYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4150..5397)
                     /locus_tag="VUW49_000564"
     CDS             complement(4150..5397)
                     /locus_tag="VUW49_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7980525.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMLLPTLFTVFAGIRADYTRAKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(5588..6439)
                     /gene="rgg"
                     /locus_tag="VUW49_000565"
     CDS             complement(5588..6439)
                     /gene="rgg"
                     /locus_tag="VUW49_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET7980526.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVCIFEMLGCNKSAEY
                     YRNTTEC"
     gene            6563..6904
                     /locus_tag="VUW49_000566"
     CDS             6563..6904
                     /locus_tag="VUW49_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7980527.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            6962..7042
                     /locus_tag="VUW49_000567"
                     /pseudo
     CDS             6962..7042
                     /locus_tag="VUW49_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7158..8861)
                     /gene="ilvD"
                     /locus_tag="VUW49_000568"
     CDS             complement(7158..8861)
                     /gene="ilvD"
                     /locus_tag="VUW49_000568"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7980528.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(9094..10026)
                     /locus_tag="VUW49_000569"
     CDS             complement(9094..10026)
                     /locus_tag="VUW49_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET7980529.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(10023..10880)
                     /locus_tag="VUW49_000570"
     CDS             complement(10023..10880)
                     /locus_tag="VUW49_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7980530.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDENKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(10884..12230)
                     /locus_tag="VUW49_000571"
     CDS             complement(10884..12230)
                     /locus_tag="VUW49_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET7980531.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVFILAVMFGVSFIKKPSAKEE"
     gene            complement(12243..12527)
                     /locus_tag="VUW49_000572"
     CDS             complement(12243..12527)
                     /locus_tag="VUW49_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7980532.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(12531..14561)
                     /locus_tag="VUW49_000573"
     CDS             complement(12531..14561)
                     /locus_tag="VUW49_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET7980533.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            14752..15759
                     /locus_tag="VUW49_000574"
     CDS             14752..15759
                     /locus_tag="VUW49_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7980534.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            15762..15962
                     /locus_tag="VUW49_000575"
     CDS             15762..15962
                     /locus_tag="VUW49_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET7980535.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            16114..16296
                     /gene="rpmF"
                     /locus_tag="VUW49_000576"
     CDS             16114..16296
                     /gene="rpmF"
                     /locus_tag="VUW49_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7980536.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            16312..16461
                     /gene="rpmG"
                     /locus_tag="VUW49_000577"
     CDS             16312..16461
                     /gene="rpmG"
                     /locus_tag="VUW49_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7980537.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(16883..18763)
                     /locus_tag="VUW49_000578"
     CDS             complement(16883..18763)
                     /locus_tag="VUW49_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET7980538.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(18757..19626)
                     /locus_tag="VUW49_000579"
     CDS             complement(18757..19626)
                     /locus_tag="VUW49_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7980539.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(19710..22355)
                     /locus_tag="VUW49_000580"
     CDS             complement(19710..22355)
                     /locus_tag="VUW49_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET7980540.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYNLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLDPQEIRTEFIKKEEI"
     gene            complement(22446..23726)
                     /locus_tag="VUW49_000581"
     CDS             complement(22446..23726)
                     /locus_tag="VUW49_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET7980541.1"
                     /translation="MVYSKEIVREWLDEVAERAKDYPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            23898..25982
                     /locus_tag="VUW49_000582"
     CDS             23898..25982
                     /locus_tag="VUW49_000582"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET7980542.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQVDWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMTPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(26022..27701)
                     /locus_tag="VUW49_000583"
     CDS             complement(26022..27701)
                     /locus_tag="VUW49_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET7980543.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQTGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            28456..29685
                     /gene="arcA"
                     /locus_tag="VUW49_000584"
     CDS             28456..29685
                     /gene="arcA"
                     /locus_tag="VUW49_000584"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET7980544.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            29743..30759
                     /gene="argF"
                     /locus_tag="VUW49_000585"
     CDS             29743..30759
                     /gene="argF"
                     /locus_tag="VUW49_000585"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7980545.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            30924..31871
                     /gene="arcC"
                     /locus_tag="VUW49_000586"
     CDS             30924..31871
                     /gene="arcC"
                     /locus_tag="VUW49_000586"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET7980546.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            32080..33591
                     /locus_tag="VUW49_000587"
     CDS             32080..33591
                     /locus_tag="VUW49_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET7980547.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            33613..34944
                     /locus_tag="VUW49_000588"
     CDS             33613..34944
                     /locus_tag="VUW49_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7980548.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(35350..35640)
                     /locus_tag="VUW49_000589"
                     /pseudo
     CDS             complement(35350..35640)
                     /locus_tag="VUW49_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35628..35879)
                     /locus_tag="VUW49_000590"
     CDS             complement(35628..35879)
                     /locus_tag="VUW49_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET7980549.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            36083..36160
                     /locus_tag="VUW49_000591"
                     /pseudo
     CDS             36083..36160
                     /locus_tag="VUW49_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(36278..37162)
                     /locus_tag="VUW49_000592"
     CDS             complement(36278..37162)
                     /locus_tag="VUW49_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7980550.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(37307..38458)
                     /locus_tag="VUW49_000593"
     CDS             complement(37307..38458)
                     /locus_tag="VUW49_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7980551.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(38604..40370)
                     /locus_tag="VUW49_000594"
     CDS             complement(38604..40370)
                     /locus_tag="VUW49_000594"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET7980552.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHTQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPIMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(40383..41111)
                     /locus_tag="VUW49_000595"
     CDS             complement(40383..41111)
                     /locus_tag="VUW49_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7980553.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSIKETKK"
     gene            complement(41126..44143)
                     /locus_tag="VUW49_000596"
     CDS             complement(41126..44143)
                     /locus_tag="VUW49_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HET7980554.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVNSYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKYTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGIYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            complement(44154..46370)
                     /locus_tag="VUW49_000597"
     CDS             complement(44154..46370)
                     /locus_tag="VUW49_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET7980555.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLTLCVPEYLTKLFVIEEVVCKY"
     gene            complement(46392..48263)
                     /locus_tag="VUW49_000598"
     CDS             complement(46392..48263)
                     /locus_tag="VUW49_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HET7980556.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEI
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKRMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTVVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            complement(48269..49588)
                     /locus_tag="VUW49_000599"
     CDS             complement(48269..49588)
                     /locus_tag="VUW49_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET7980557.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            complement(49585..50424)
                     /locus_tag="VUW49_000600"
     CDS             complement(49585..50424)
                     /locus_tag="VUW49_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7980558.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            complement(50427..51356)
                     /locus_tag="VUW49_000601"
     CDS             complement(50427..51356)
                     /locus_tag="VUW49_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7980559.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIVCINILFRERKRLKGGK"
     gene            complement(51443..52735)
                     /locus_tag="VUW49_000602"
     CDS             complement(51443..52735)
                     /locus_tag="VUW49_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7980560.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVQTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            complement(52764..54167)
                     /locus_tag="VUW49_000603"
     CDS             complement(52764..54167)
                     /locus_tag="VUW49_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET7980561.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLVDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEGTAVGNIVVQLLSQGKINEDTELK
                     DIMTNIAETQYYYPQLS"
     gene            54343..55116
                     /locus_tag="VUW49_000604"
     CDS             54343..55116
                     /locus_tag="VUW49_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7980562.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPNQKLLDAVELFDGEWVSDIQ"
BASE COUNT        17156 a        12036 c         9799 g        17228 t
ORIGIN      
        1 acaaaaatcc gatgaacttc accggactct tttattttga atttttgcct gctttacgct
       61 tttcagcgat ttcggctgcc tttcgaggca agacaatttc cgttatgtaa gccgtcccaa
      121 aacgcagtac acctgcaata ggagcaaaga caactgctag atagttatag aagaaatcgc
      181 ctttgaaggc ataagctagc gctccaatga tgaaaaatag aacgactgcc tgaatcactg
      241 ctaataaaat tactcgtttc atgtgacctc ctgactctat tatagcatga gaatcatcaa
      301 aaagccgact aaattattca aagcgtgaag agaaatactg tagaccagac cttttctgct
      361 aacgtaagcc aaacccaaac taaaaccaag gctaaaatag acaaaaaatt gttgcacatc
      421 acctggaaaa tgaatcaagg caaatagaag actagatacc agaagaaaaa tcagggttcg
      481 tttactattg tcctgcttag gaaagagata gcgtgctaac atccctctaa aaacaatctc
      541 ttccgtcaaa ggagcaaaaa taaccacagc aaagaatgag aaaagtggtt gagacaaggt
      601 caagtctgtc gctatttgct gatttactga aggatcatct ggcaagaaga attgaacgac
      661 cagagataag aaccaaacca aggcaggaag ccaaataaat cggttaaagc cgctcttctc
      721 aatataaaca ggagctttct gataccattt gtaaatgccg tacacatata ctccagccaa
      781 gaccacatag agtagagtaa cagcataggg tgaagcgcct aaagcaagcg acgcagtcgc
      841 gagcccctga ataaagccat agataaagaa aaaggataga agggctagaa gaatccagcc
      901 aaggttttta agtaatttca tagataactc ctttatttga aataacgttt taccataggt
      961 aactgcatca cattgatata aacatggatg gctcctacaa gcaagaaagc tagtaactga
     1021 atctctcctg tcaagaaaga aatgataata agaaaaatat ataaggctgg taagacatat
     1081 tggtgtaatt ggaataaaat tcgaaaactc tgttccaaat tagcctgacg ctccccttca
     1141 tcataaaaat ttatatagtt caagacatcc tttggtgtag cgaaaaattc caaatcaaac
     1201 tgacgaacaa tcgcaatggt tttaaaaaga gatttttgag cgactaagaa taccacaaag
     1261 agtaagaaag aaaggaaaaa tgtttgaggg tttgtatgca atataatcac ctcacttaat
     1321 gaaataaaaa tagccaatgg aatcgctaca cctgtaatat taaaagcaat ggttccaaac
     1381 tcaagattcc gatacatttg cacataatag gtttcattca gatcgtcatc catttcctct
     1441 tgatacaagg aatgaaattt tctgcttttc tttaagaaat tgaaagtcaa aaacatacta
     1501 atgaaaccta tcagtaaaca aatagctgat atccatggca tcaaggcttt tacatctaaa
     1561 ataatttcgt gggattcgac acgtgcctta aacatcccta caaacatgcc caagaacccc
     1621 cccaagacaa tagacatcaa aaataacaat ctacgtttct ttttcatatt cattctcctt
     1681 tttcacttgc tagatttttg gatttctttt caatccattc agttactggg atgagagcaa
     1741 agtagaccca aacaaattgg tcgctttgat agggattaaa ccagcttagg tccatcccaa
     1801 tcagtagaaa tacgctgact aataaagcta tgaccactac ataataaatc actttatact
     1861 tgttcatcac tcgtcctcct ccaaacgaaa taccgattcg actgtttcgt tgaaaatttg
     1921 agatattttc agggcaatga taatggatgg ggtgtactca tcccgttcta gtaggctaat
     1981 ggtctgtctg gaaacccctg ccagtttggc taggtcggtt tgattgagac catcgcgagc
     2041 tcgaagctct tttagacgat tttttagttg cacgttacac acctactctc cgtcaaattc
     2101 aacggtttgg atatcttcaa tacgttgcaa cttgaatttt tcttttcccg tattatcaac
     2161 actacgtagt ttgacccatt cctcatcaac atccacaact tcccagttat ctggcccaat
     2221 atacactccc gttataattg gttcctttcc aatcatttct tgtaataatc tcgacatttc
     2281 tgcgtttcct ttctcttttc gctcaagttt tttgatttta ttctctagtt tcttgatttt
     2341 tttagaatta ttagaataaa agaaaatcat aaatagtata aatcctagta cccacattat
     2401 aactcctttc tgcttcctat ttcttaactt gaattcattg taacatatct ttttcttttt
     2461 gacaagtata tttgtcaaaa agtttatgat ttttgtcatt ttgcaaaaga aaaaggtcag
     2521 gagtaagttc ctgaccactt tatctatcat taatactctt cgaaaatctc ttcaaaccac
     2581 gtcagcttca ccttgccgta ggtatggtta ctgacttcgt cagttctatc tacaacctca
     2641 aaacagtatt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaacc atgttttgag
     2701 ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagcaacc tgcggctagc
     2761 ttcctagttt gctctttgat ttttattgag tataaaatcc tagtttttca aagatttctg
     2821 agaagttttg gctgattgtc tcaagtgaca cttgcacttc ttctcgagtt tggttgttct
     2881 tgaccgtcac ttgtccgctt tcgacttcgc tctctcctag ggtgatgagg gtcttagcca
     2941 caaagacatc ggctgacttg aactgagctt ttagtttacg gttgaggtaa tcacgctctg
     3001 ctttgaaacc ttgttggcga agagcctgta ctaattccaa ggccttgata tttgcccctt
     3061 cgcccaagac tgcgatatag acatctaggg cgttttcgat agggagggtc acaccttgct
     3121 tttcaagaat gagaagcagg cgctctacac caagtccaaa accaaatcca gcagtttcag
     3181 ggcctccaaa ataagaaacc aaaccatcgt aacgaccacc cgcacagacg gtcaggtcat
     3241 tgccctcaat ctctgtgata aactcgaaaa tggtgtggtt gtagtagtcc agaccacgca
     3301 ccatattggt atcgatgatg taatctactc caagattttc caacatctga cgcacagcat
     3361 caaaatgagc ttggctttct tcatcaagaa agtccaagat agacggcgca ttctctactg
     3421 cctccttgtc ttctttttcc ttagagtcca agacacgaag aggattttcc tccaagcgac
     3481 gttggctatc cttagacaag gtctccttga gcggtgtcaa atagtcaatc aaggcttggc
     3541 ggtaggctgc acggctctca ggatttccaa gagtgttgag gtgcaatttg acaccttgaa
     3601 taccgatttc cttcaaaaaa tgggctgcca tagcgattgt ttccacatcg gtagctggat
     3661 tgctagagcc aaaacactca acaccaattt ggtggaattg gcgcaagcgc cctgcctgtg
     3721 gacgctcata acggaacata ggtcccatat agtagaactt gcttggcttt tgcacttctg
     3781 gggcgaagag tttattttcc acataggaac ggacaacggg cgcagttcct tctggacgga
     3841 gggtaatatg acggtcaccc ttgtcataaa aatcgtacat ttccttggtt acgatatccg
     3901 ttgtatctcc gacagagcga ctgataacct cgtaatgctc aaaaataggc gtgcgcactt
     3961 ctgcatagtt atagcgcttg aaaatctcac gggcaaagcc ctcaacgtac tgccacttag
     4021 cagattcagc aggtaaaata tcctgcgttc cttttggttt ttgtaatttc atagggaatc
     4081 ctctttaaac ttaatagtct tattttacca caaataaagg gattaaaaca gtaagaaaaa
     4141 aattaggatt tagatatcat ttttgagatt aagaattgtc aaaaaaatag ctagcaagga
     4201 aagaccaaca aatagcatcc aagtcaactg tatattccat acggctacta gtgaaaaaca
     4261 agctgttccc acaggtatgg ataaggtaaa caatagacct aaaaaattac tagtacgagc
     4321 tagaacctct ggagctagat ttttcatgag catggcacta atctttggtt gaactttacc
     4381 agacacatac agggtaaaga agagaaatag caaaccaagc acgacttgat tgaataaatt
     4441 agccaaacca actagactaa gtcctacggt cgcccacatc atcaatctag gcaaggactg
     4501 cttcccaaaa taatcattgc ccgtaaggct actgatgatg attgctacta aaacacagaa
     4561 ttgattgata aatagtgcct ctgtataaga aaaattcaag agagaatggc tcaaaaagaa
     4621 gatattataa attccaccca aagcgccacc caaggaatta ataagcaaga cagcaaagag
     4681 cataaaacca aagtttttct gtccactttt aagaaaaacg agacgtaaat ttcggtaaat
     4741 tgttaggaac tggtctttga tagaaagctt ctcatttttt aagttttcac catcagcaga
     4801 tgacattgac aggctcaatt tgctttttcc taaaaagagg atagtggctg atactaggaa
     4861 aaagcaggca ttgattcccg caacgagaga aaaattgttg accgatagag ctaagagcca
     4921 gactccgaaa gcttgaccac caatagctga aatataggtg atgaactgtg aaaaagaata
     4981 agcctccatc aaatcatctt cagctacttt ttccttaata agaggcatac gcaggccacc
     5041 tgcaaaatca ctgatgatat cactaatgac attgatcaaa cacaggctag aaaaggcaaa
     5101 taaactagct tgctgaacaa ctagggctgc cagaaaaaat aaaaccgcct gaaacaaacc
     5161 gctatagacc atccatttga ccttggccct cgtgtaatct gcccgaatcc ctgcaaaaac
     5221 tgtaaagaga gtcggaagaa gcatgacaat attcgccata gcaacagcaa aagatgcttg
     5281 tgacaaggtc gatgcataga cgataaagac cagattgaaa atcgaagcac caaaagcatt
     5341 gaagaagcgt gataaagtca aaattctata agctggattt ctaaacaata gtttcataga
     5401 taatctccct ccttgttcta agttctacat caagaatagc tttagtatag cacttatgaa
     5461 tttgcgaaac gattcttttg aatatatgca acaaataaaa tgagcaaaaa aacgatagaa
     5521 cttttgtaat gacctgagag agggcaatag ttctatcgct tcaaattttt tcttggtttg
     5581 cagatattca acattcggtc gtatttctat agtattcggc agatttatta cagccaagca
     5641 tctcaaaaat acagacagca tcctccatct ttttctggcc ttccttgact ctaccttgct
     5701 tgctatcaag gagaccttct gcccacagat aaacaattcg gaaataggtc tcattttcct
     5761 tgtagaaatg ctcttcgata acacgtttaa aataataggc attggtaaat tcttcacact
     5821 caatactagc taaaaagcca ttcaatagta tagtatgaaa aaggtttcga ttgccagaca
     5881 tttccattag aaaatcagat ttacgtacca tttctcgtac atatctagta aaaagagaaa
     5941 cagataaaaa tggagaactg actgaaaata aattgagttc atagattccc cagatctcgg
     6001 tagaaaacaa ataatcatga aggacttttc cttcctctgc tgttaactct accctttcat
     6061 ctatgctctt catataagac ttgatcataa tggcatttag aatatgtttc tgtttgttgt
     6121 gagaatgggc atgcttttga tactccctgc gatataagtc ctcaagaggt gctatattct
     6181 ttggttccaa gacatctgta atttcttttc tcaactcaga atctgtatca tactggaaac
     6241 ctctcgccag aaagaggatc tcctccacac tggcagatat attttccaga gcaaatagaa
     6301 acttttccac cgaaagctca ctctgacctg tttcaaaacg ggacaacata gacggcgaaa
     6361 attgtcctcc ggttgcttgt ctcagtgaga tatttcttga ttctcgtaat tgtctaaaga
     6421 cttttccaat ctgctccata gacttcccct tgattccgta ttttcttcat tttatcatat
     6481 ttttcagaaa attcatcaaa aacttgccaa attgtcagaa ttatgagaaa atagaggata
     6541 tttatcacgt ggagggactg ctatgagaga cgatatcaaa atcaatgacc gtgctttggc
     6601 cttgcaagac caaatcatcg aaaaactaga gaaagttttt gatacagatg tggaattgga
     6661 tgtttacaat ctaggtctga tttatgaaat caatctggat gaaacggggc tctgcaagat
     6721 tgtcatgacc ttcaccgata ctgcctgtga ttgcgccgaa agcctgccta ttgaaatcgt
     6781 ggcaggtctg aaacaaatcg agggtatcaa agatatcaag gttgaagtta cctggtcgcc
     6841 tgcttggaaa atcacacgaa tcagtcgcta tggccgtatt gcccttggac taccacctcg
     6901 ttaagcaggc caatcacttt taaagatgaa aaaagcaagc caaaaatggc ttgctttttg
     6961 atactcaatg aaaatcaaag ggcaaactag aaaactagcc gcaggttgct caaaacactg
     7021 ttttgaagtt atggatagaa ctgacgaagt cagctcaaaa tactgttttg aggttgtgga
     7081 tagaactgac gaagtcagta accataccta cggcaaggcg acgttgacgt gatttgaaga
     7141 gattttcgag tatgagttta ttttttacct gacttgtcca tattccagaa gtctgtcacg
     7201 gctccgcgtg aagcagatga tacgatgtgg gcatatttac cgaggacacc acggctgtaa
     7261 agtggtggca aggttgtttc tgccttgcgt ttttcaagtt cttcttcgga tacggccatg
     7321 gaaatttctt tggtatcttg gtcaaccgta acgatatcgc cggtacggag ataggcaatt
     7381 ggtccaccat cctgagcttc aggagcgata tgtccaacaa ccagaccata agtaccacca
     7441 gagaaacgtc cgtccgtcaa gagggccacc ttatctccct gacctttacc aacaatcatt
     7501 gaagaaagtg acagcatctc aggcatacca ggaccacctt taggtccaac aaaacgaaca
     7561 acgactacat cgccatcaac gatttcatct gtcagaacgg cctgaatcgc atcttcttct
     7621 gagtcaaaga ccttagctgg cccaacgtga cgacgcactt taacacctga taccttggca
     7681 actgcaccgt caggagcaag gttcccgttc aagatgataa gcggaccatc cgcacgtttt
     7741 ggattttcaa gtggcatgat aactttttgg cctggagtca agtctgcaaa gtcagccaag
     7801 ttttcagcta cagtcttacc agtacatgtg atgcgatctc cgtgaaggaa accatttgcc
     7861 aacaagtact tcataaccgc agggacacca ccgacttcgt agaggtcttg gaagacatac
     7921 tgaccagatg gtttcaagtc ggccaagtga ggcacacgtt cttgaatcgt attgaagtcc
     7981 tcaagtgaca agtcaacatt tgcggcatgg gcaatggcga gcaagtgaag agtggcgttt
     8041 gtagaaccac cgagagccat cgttacagtg atagcatctt caaaggcttc acgagtcaag
     8101 atatctgatg gtttgagacc aagttccaac atcttaacaa cagcacgtcc tgctgcttcg
     8161 atatcttctt tcttatcagc tgattcagct gggtgagagg atgaccctgg caaactcatc
     8221 cctagaactt cgatagcagt tgccatggta ttagcagtat acataccacc acagccacca
     8281 gggccagggc aggcattaca ttcaagacgt ttcacgtcct cagctgtcat gtcaccgtgg
     8341 ttccattttc cgataccttc aaagacagaa accaagtcga tatctttacc atcaagattt
     8401 cccggtgcaa tagttccacc ataggcgaaa atagctggga tatccatatt agcaatagca
     8461 atcatagatc caggcatgtt cttgtcacag ccaccgatag cgacgaaggc atccacgttg
     8521 tgaccactca tagccgcctc gatggagtcc gcgatgatgt cacgagatgt tagagagaaa
     8581 cgcataccag gcgttcccat agcgatcccg tccgctacgg taatggttcc aaactgtaca
     8641 ggccaagcgc ctgcagattt gacaccttct ttagccagtt tcccgaaatc atgcaagtga
     8701 atgttacatg gtgtattttc cgcccaagtc gaaatcactc ccacaatcga tgtttcaaag
     8761 tccttatctg tcataccagt cgcacgaagc atagcacggt taggtgattt aaccatgctg
     8821 tcatagatgc tactgcggtg acgtttatct aattcagtca tcttatccct cccatttcag
     8881 tttttactat tatagcacaa tttaaaggca atgaacagaa taaaattctt gaattttcag
     8941 aaaattctat acacatgtga aatatttaaa attaaaaaca acaaagcgga ttagtgcact
     9001 ttctgatgac cagaatatgc tttttaatcc gctttcttta aataacgtaa tgtaattttt
     9061 acagaaattc tttcagataa gtgtatttaa catctatctt gcattataaa tttctagaac
     9121 cttctctttt atattcgatt cactcaaacc atactcatta agaagataat ccattttccc
     9181 tacttgaccg aatctttctt gaacacccat ccgatgaatt tttgttattc catcatcaga
     9241 gaataattca cataaagcac tgccaattcc acctatctga ttgtgatttt ctacagtaaa
     9301 tatagttttt ccacttaaca ttgtttttat ctgttctggt atcggtttga ttctaaataa
     9361 atctatcaca cctactgaat aacctaattt agacagttca tctgcaactc gaatacttgg
     9421 agcaaccatt atgccagaag caacgattac aagatcttca ccatgcctta actcaatgta
     9481 gcctttagaa aaatcttctc caccttgata cacaggaact ggagcttttc taattgttcg
     9541 aatatatttt agtcctttta agtctaatgt ctggttcaat atttcacgaa attggatatc
     9601 atcagttgct tcgaaaatga ttgatttagg aattaaacgt aacaatccaa tttcttcaaa
     9661 tggcatatgt gttccaccat tcatctctgc cgttactcct gcatctgatc caatcacagt
     9721 ggcatccaat tgtgcgtatc caagagaaat aaataattga tcaaatactc ttcgtgaagc
     9781 aaaaggacca aatgtatgaa gataaggtct aaacccctga atagacaagc ctgctgcaag
     9841 gccgaccatt tctgcttcca taatcccaac attcacataa cggtctccga agtccttttc
     9901 aagattatga gtagccatcg aacttgacaa atcggcttct aagactacta tatcagaata
     9961 actttgatta gcctctaaaa ggaagtctct atatacatgc cgtaattctt tcgtacttct
    10021 catcattctg tttcctccaa ttcctgactt aatctttcta caactgaagt taacatttgt
    10081 ttctcctcta cagtagggcg aagatgatga ttggatttca tttcttccag ctcttgaacc
    10141 ccttgacctt taatagtatc taatacaatg cacttaggtg atgaattatt tgactgtttt
    10201 aattggacaa tcccttcata aatttctcta atatctgaac ccttgaccct aatggattca
    10261 aatccaaatg ctgaaaattt ttctacgaaa tcacctggat tacaaatatc ctttgtaaaa
    10321 ccatctaatt gttttttgtt ctcatcaaca aatacaatta agttggataa ctgttgatga
    10381 gaagcaaact gtatagcctc ccaacattgt ccttcattta actcaccatc tccaacaata
    10441 gcataagtat aaaagggact ctttcttatt ctctgaccat atgcaagtcc agttgcaaca
    10501 ctaattcctt gtcctaaaga gcccgttgtc atatctatgc ctggcgttag atttctatca
    10561 ggatgagacg gtaatttggt tccatttgta tttaaagaat ataagaattc tttgtcaaag
    10621 aaaccattca aatagagtgt actgtataga gctggtcctc catgaccttt tgataatatg
    10681 aaataatctc tatctcgtgc tgcaaatatt tctggagtca ttggcattat ttcaccataa
    10741 agcactgcta aaacttctac gatagacaga cttcctccgt aatgtccgaa tccaagatga
    10801 ttcaatgttc taagagtatt taatcggatg ttagtcgcaa attttcttaa ctcatcttct
    10861 ctatttttac ttaaaatcat cccttattcc tcctttgcag atggcttttt aataaaggat
    10921 actccaaaca taactgctaa aataaaaaca agaccaatca caatgcctgc ttgtgagcca
    10981 aattgattta acattcctaa aataattcct gatagaccaa aatctgcatc tgagaaagtt
    11041 gatccttgga aaccgagtcc tcccaaaact ggcattaaaa agactgggag aaaactgatt
    11101 aaaatacctt gtaaaaatgc tccaatagtg gctccacgaa caccaccaga tgcattccca
    11161 ataacacctg cagtcgctcc acagaagaaa tgaggcacaa cgcctggtaa gataacaacc
    11221 gttcctgaag caatcataat tgccatactt actaaaccac caataaaact agagataaat
    11281 ccaattagaa ctgcattagg tgcataagta taaacaatcg gacaatccaa agcaggtttt
    11341 gaattaggta caagacgctc tgaaatacct ttaaaggctg gaacaatttc gcccaaaata
    11401 aggcgaacac ctgctaaaat aacaaatacc cctgctgcaa attgacctgc taattgtaaa
    11461 gcataaacta gaccacttgt accactactg atttcttttt ctatatattc tgaccctgca
    11521 aagatagcta caataatgta aataactgcc atagataaag tgatactaac agtactatca
    11581 cgtaaaaaag ctaaactctt tggaaattta atgtcctctg ttgattttga tttgtcaccg
    11641 ataaggctac cagtaaaacc actcaaccaa tatcccaaag aactgaaatg acctaaagcc
    11701 accttgtcat ttccagttaa ttgaaccata tatttttgca caaatgctgg ggaaatactc
    11761 ataataatac cgagtgctaa tcctcctagt aagatgagag gcaagctagt aaagccagca
    11821 actgataaaa tgaccgcaat catacatgcc atatatagag tgtggtgccc tgttaaaaaa
    11881 atatatttaa atcgagtaaa acgagcgatt aagatattga acaccattcc tgcaaacata
    11941 atcattgcag tagctgagcc atatgttgtt aaagctacag ctacaattgc ttcattattc
    12001 ggcacaacgc cagataaatg aaaagcatgc tcaaacatgg taccaaatgg atttaaagaa
    12061 ttttgtacaa ttcctgcacc accagataca actaagaaac caacaaaggt cttaattcca
    12121 cctttaataa tatcaggtaa tttcttcttc tgaagaacta atcctaaaat tgcaattaaa
    12181 gctactaaaa tagctggtgt actaacaata tccaatatga acttcatcat gacgctagcc
    12241 tcctatataa gtcctttttc ttcacaaagt ttagtaatta attctcgtag ttcatccata
    12301 tcaataatac tatttaagat acgaacatct ccaagatgac tagctgaatc agctagatca
    12361 cgaccaacaa tccaaatatc agctgcattt ggatctgctc cacctaaatc ataatgttca
    12421 acttctacat ccgaaacatt caaatcactc aatacagatt caatattcat ctgtaccata
    12481 aaacttgaac ctaatcctga accacaagct gtaccaattt ttaacattat ctaatcctcc
    12541 tgtttaatta tcattttaat atcattatag ttttttgatg atattaaagt ttgaacatga
    12601 tttttatctc ttaaaattgt tgttaaatgt gacaaagcct ttaaatgact ctcattatca
    12661 atggctgcaa tacaaatcaa caatcttacc tcttgttctg gattatccaa taaataaatc
    12721 ggttcttcca aaactaacat tgacattcct atttcattca caccttcatc tggccgagcg
    12781 tgaggaattg ctactccctt ccctaaatta ataaaaggtc caaactcttc tactttttga
    12841 atcattgcct cagggtagtt ctcagttatc ttatcttgat ccaaaagcgg tttagctgct
    12901 aaacgaatcg cctccttcca tcctaatttt tgcgaactaa cctgataggt ttctttggta
    12961 ataagttgtt ctagcactgg tacaatttcc tttctatcat ttttttggta aagataattc
    13021 tttaacgcca atcttaattc caattcttgt gtaataattc catatctttt gacaatattc
    13081 aggatttgtt caatctcaaa atctccatac tctaaattcg gaaaatcttt taacactagt
    13141 tctactagtt gtattgcttg ctcttcagtc atcataaccg aaactagata atttggcttt
    13201 tctgtctcca cctttatggt agaaaaaacc atatcatagt cactactagc tttcacctgt
    13261 aaatcatcaa tctttgaggt tcctataaac tcaatttgag gaaataatgc taatagattc
    13321 tcttttaaca tcaatgaaga actaacacca ttaggacaaa tgattgctgc tttataccat
    13381 ttttgaggca aagtatctgc tttctttaaa taacctccga aatggataac aaaatatgct
    13441 gtttcactat caggtatagg attgtcaata gcgtccatca agggcatcaa agaatctttg
    13501 actaattcaa ataaatcagg ataatgttct ttaacatgca atacatattc atttgaacta
    13561 ggtaggccga actttaatct atagtaagcc ggtataaggt ggcggcgaag attttctctc
    13621 aatccttccc tttgtttaaa atgtaacaaa gaaatatctt ccattctact tataatagcc
    13681 tctgttaatt gattaaagta aaccggagca acatctactt caccttcaaa gcaacttgat
    13741 aataaaacgg tgatatagcg ataatcatcc tcagaaaaca ccgtatctat aattcccaaa
    13801 tcaaccactg tatccaataa aatagtggtt atatcttgaa taacaggaga tactaatgtc
    13861 tctgaaagac atactctttc aacatccctt tgatacctac acagaatgaa tactaaaccg
    13921 aaaaggtaaa cttttaattg attaacaata ggtactagct gtagcttctc ataataatct
    13981 ttaactacct gatcaatcaa atcataagtt aatgaatacc cccaactgga taaaacataa
    14041 tccaaacccc aaatccctat ggaggattcc agcaactcac taaccatttg aaaagctaag
    14101 cggtgcttat tccactctga accgtgtaaa gtataacctt ttgctctact gtaccctagc
    14161 tccaaatcat tatctaacat aatctttctt aatgattgaa tatcagataa ggttgtattc
    14221 ttacttactt tcaaaaagtc ttggtaatga ttattcgata taaaatctaa tcggcaaaaa
    14281 gtgtaaagat agattaaagc taagcgagtc gactttggta aaaccaattc atccgactta
    14341 ataatatctg tcaaagactg cttcgtacga tttgataaac tatagcgacc ttgtttttta
    14401 tccagcacta tccctttatt agctagataa ggcactaaat aatctattcc ttctttgact
    14461 tcctttatag gtaagctcac cttaacagat aattcatata atgatagctc acaatgatcc
    14521 atcaaagtca tcaaaataac tagtgctcta taatcaaaca tttctcttcc tttctaacta
    14581 caattatagt gtaaaaatta taagaataaa agagtatttg agcacaatat aatctcacct
    14641 tttaaaagaa attattgggc tgaaagtttt tagtgattat tttcaacttt ctattgactt
    14701 ttaaaaacaa agtgatatca tttagatatc aaataatggg aggtcattct tatgactgaa
    14761 aaactaatca attcaaaacc aaatggtgta ttcgcattga ttctcattga gttgacaatc
    14821 gtacttggta tctttatatt tataatgggt gttggttcgg aaaacatttt tggaattatt
    14881 atcggacctt tactaatcgt aattgcaggt ctagctcatg ctggtttaaa agttgtcaaa
    14941 cctcaggaag ctctggttct gacactcttt ggtaattata caggtaccat caaagaacct
    15001 ggtttttact ttgtcaatcc cttcagcgta gcagtcaacc ctgcaaacca cactcgactt
    15061 ggacaaagtg gtgatgttag cacaaaatct ccttttttag gagctaaatc atcaaatgac
    15121 aatgatgtaa atcttgaaat tggcaagaaa cagatttccc tcaaagtcat gaccttgagc
    15181 aattctcgtc aaaaaatcaa tgattgctta ggaaaccctg tagaaatcgg tatcgcggta
    15241 acttggagag ttgtcgatac cgctaaggca gtcttcaacg ttgataacta caaagaatat
    15301 ctttcattgc aatgtgatag cgccctccgt aatattgtcc gcatctatcc ttacgatgtg
    15361 tctcctaatg tggatactac gggtgacggg caagcagatg aaggtagtct ccgtggctct
    15421 agcgaaattg ttgctaaccg tattcgtgaa gaaatccaaa gtcgtgttga ggatgctggc
    15481 ttggaaatcc ttgaagcacg tatcacttac ctagcttatg ctccagaaat tgctgccgtt
    15541 atgcttcaac gccaacaagc atctgccatt attgatgcac ggaagatgat tgtagatggt
    15601 gctgtaggaa tggttgaaat ggcactagaa cggctcaatg aaggggaatt ggtagaactt
    15661 gacgaagaac gaaaagctgc catggtttca aatctccttg tcgttctttg tggcaatcat
    15721 gatgcacaac caattgtcaa cacaggaagt ctttactaaa gatggctgaa gctaaaaaaa
    15781 agcagatccc ccttcgactc tcaacaaagt tatacgctgc actcgcatca tgggcagaag
    15841 acgactttcg ttcagtcaat gggcaaatcg aatatctcct cacagagtgt gtcaagcaac
    15901 ggaaaaaaga cggaaaatac gtatcagaaa ccattgacga accatttgag attgatattt
    15961 aacaccttct cctgtctgcc gtgtcggata ggagaatttt ttcatctttt tttgtcaagt
    16021 aactttactt tacaaaaaaa atgtgttatc ctagtatggt tgatgaaaat cagtagattg
    16081 aatcgaatat aaatacctaa aggagaaatc aaaatggcag tacctgcacg tcgcacttca
    16141 aaagcgaaga aaaacaaacg tcgtacacac tacaaagtaa cagctccatc tgtaaacttt
    16201 gacgaaacta ctggagatta ctcacgttct caccgtgtat cacttaaagg atactacaaa
    16261 ggacgtaaaa tcgctaaagc tgcatcagct gaataataga agggagatac catgcgcgta
    16321 aatattacac ttgaacacaa agaatctggt gaacgcttgt accttacttc taaaaacaaa
    16381 cgtaacactc cagaccgcct tcaattgaag aaatactcac caaaacttcg caaacacgtt
    16441 gtgtttacag aagtgaagta agcattcaat acgaatcaat acagaagaaa aacgcttgat
    16501 cagcgttttt tctttttgtt aatttcacta tattgcaagg gaaatcattc gaatgactac
    16561 attattgata atatttgtta agccatcaaa agtaattaac atttcatagc gagtaatcct
    16621 atttaaactc gtcccactat ctatttcttc ttatctctgt gttataatcc cgactttatc
    16681 ataagtttgt ctaactgtag catttgaaac cagatacttc ttctgtttca cgcgaaacac
    16741 cctgcgccaa gcaaggtgtt cttgttttta tactcaatga aaatcaaaga gcaaactagg
    16801 aagctagctg caggttgctc aaaacactgt tttgaggttg cagatggaag ctgacgtggt
    16861 ttgaagagat tttcgaagag tattaagtcg tataaatcgt cgacgctgtc gcaatggtat
    16921 gccactggtt ggctgtagtt gctgcgaaat ccttgtctgc gtagaagtca gtaaatggta
    16981 ggatttcaac ttccagctcg tcgatgcggt caagctgacc agccagataa acctcgatac
    17041 ggctttctgc tcgtttgatg cgttgcaaga gtccgcccat acggatgtca actgtatcca
    17101 aaccaaagac cttgttttct ttcagccatt ggtgactaaa gagggcatgg aagtcttcaa
    17161 tttggcttct aagttctggt aattcttgtc tggcgatttg ttgtaaactt tctttatcat
    17221 ccgcttggta ggcctgacga atgcgtcgtc ccacatctac tttgctactt aaaatagcat
    17281 tcaactgggc ctgagtttca aagagataag catagtttcc agctttttct ttaatgtcag
    17341 caatagttcc cgccgcctga gcgaagtgtg gcttgtcctg ttcaggtgtc atgtgtcggt
    17401 caagtatcgg acagagaaca tcctgataaa agacatatcg gttgggattg atgccactga
    17461 gattacctgg taggtctggt aagaggttgg caaggtcaat ctgcataaaa tcttcaaccg
    17521 ttagaccagt attggtcttg aagtgcgcag acaaactatc taggtcattg cgatagctga
    17581 gttctgccca gatttgcaag cttggtagga tagagaactg ggcagtttca ccaccattgt
    17641 ctccccaacc cgttacgatg acttctttaa tctgattggc acggcaggct ttattagcct
    17701 cgatagccac tagacggcta aaatggttgt gaggtgtaaa gccaatccac ttccaagctc
    17761 cccctgcaaa tgcaaggtca tggctaatct tgtgatgatt gcggaaatta cggttgtatt
    17821 tttcctcgct atcctgataa taatcccagt aaaccagagt cacacggtct ttgagacggt
    17881 ctaggtagac acgagtttcc tctggaattt ccacatcacg atcgtactgg ccatccgctg
    17941 acatgagttt gaagaacata tcactccaca tctggcagtg gaaaccatat ttgtcagcaa
    18001 tatccagcac gcgctccaag tgttggcaca tgaggagact acgatccaca acaccgttca
    18061 aaatcaggta gcgtcccaaa ccaaccaagt gggcttcgtc catcccgata ttgaccttgc
    18121 gagtcttcag tttagacaac gtggcaaaca tgccatcaat caggtcataa accttttctt
    18181 cgccaatgag aagaatgtcc tctacatcac ggagctcctg cacttccttg acaccccatt
    18241 tgacaaaggc cgacaagtgg gccaaggtct ggatgcatgg tacaaaggtc atgtcaaact
    18301 gttgagcata ggcttcgatt tcctgcaact cctctgctga ataagctcca cggaaatagc
    18361 caaagtaagg ctgcccttca atctggtaag tgtcttccat gtaaagctca aaggttgagt
    18421 agcccatgag agccaagacc tcaatcatct gcttggcaga agcgacattc agcaccgcat
    18481 ttcgagaaca gtcaaccatg taagccaaat cttcgtaagc tgcttgttcc tcaatctcta
    18541 ctttatcagc ttctgctaga actgttacca acaaggataa ggcacgataa agttggtgag
    18601 gtttgcggta ggtcaattga tagtgacctt cctcaccctc gatagagata gaggcttggt
    18661 cagactgagt gacagccact tccacatctg gtagagaaat atgacctttt aaaacctcaa
    18721 tagcttgcgt ttgtttggga ctaagtcctg taaatcttac cattgctttt cctcctgtag
    18781 ccagttgaca agagcaccgt agagattggc atctgcgtga taggtgcagg cctggataac
    18841 tggtgcgacc gtgtattctt cgtaggtatc gacaaagtta tcaacagcct tcttgacatc
    18901 ttggataaaa tctggatttt gactgataga gccacccaga ctgatgacat ctggatcgat
    18961 gagatactgg atattgagca agccttgcgc cagattgcgg ttcatgcgct caatggcttc
    19021 ttgacaaaga gcattaccag ctgcggcctc ttggtaaatc ttgcgaccgt cccaatcagt
    19081 atgaccagat ttttcaatca cgtatcgtac catattccca gttgatgcta gttgcgacca
    19141 gttattaagt ttttcagcag gggcaagggt tgtcatgtag ccaaattctc cacccaagcc
    19201 gtggcgacct cgatgaagtc taccattgat aatcatggct ccgccaatcc ctgtcccaat
    19261 cacgacacag gctgcatttt caagatctgg atgagctagt agttcactga gtccaacgca
    19321 gttggcatca ttttctaaat ggacaggtag ctgataagag ctaagcgcct cataccaaga
    19381 aaagccatgg atgtagggca ccgcactgaa gccatcaatc acacctgtct cttgattgac
    19441 tgcacctgga acgctcatag caatcccact gtaatcctgt tctgacaagc gttgatctag
    19501 ccacgctagt aaatcctcca agttttcagg cgttgaaata cttgtcttat ccagtatttt
    19561 cccatcagga gtcagactgg caaacttaat cccagtccct ccgatatcaa tcgttgcaat
    19621 ggtcattgtt tttaccataa aaaggggcga atcagcagtt agttcttact ttttcgcccc
    19681 tcctttcttt tgatgtttac tttttgaaat taaatttctt cttttttgat gaattctgta
    19741 cgaatctctt gtggatccaa tagtcctgaa tgaactggat atggtcgctc aagtaagtca
    19801 agaatggttt gttgatgttc tgatatgcgc acattttctt gactcatatt atagtaacgg
    19861 aggacatagc cttcttcatt ttccgccact ttaaaggctg ttggacaaac ttgcggtatg
    19921 ctgagaacag aatggctcaa gaggctacca gtcgcaacca cgcttccttc ctgtctagca
    19981 agctgaaggc tggtaaacgg tgtctgcaag gctttggcac gacgataggc tgagaaacgt
    20041 tcttgggctt ggtggcattc aatcgcaaat tcgacttcaa actcccgcaa gcattgtgct
    20101 tctggcgttg ggaagtagcc ccagtcacct agctcacctg atgcacgcaa aatggtcacg
    20161 gcaatggtgt tatccccaag gatttcgtat tcattcaatc ccttattgga tacagtcacc
    20221 cctttttcat cgtcatacag actgacaaaa gcttgttggt gttgaggatt ttcagggttt
    20281 tcccatgaag cagctggttt gtttggtcgt gtcaccacct catagatact ttcagaatca
    20341 ttgcttggac gcgtgttatg agtcttgacc aagagacgga tacggtgatc cttggcagtg
    20401 ttagtaaagc gagtcttgaa gcggatttgt ggattgtcaa cgaagacagt caactcagtt
    20461 tccagaggaa tgtttgtcaa ttcttctgac cgtccagcct cacgcttcat aaactcgatg
    20521 ataccttgtt gctcttcttc tagcttttca tccgcactga caggcacggt caattcatgt
    20581 ttgagcaaga ttttagcata gcaagctgtg ttttccaaga cctcgtggcc cttaagctct
    20641 gcaaagattg gctctgttcc ttttggttgg aaatagatat actcgtttcc gatgtcccca
    20701 cggtcttcaa agcggataaa gtcttcatag gcttcgtgag ttgtcttgtc atagactgtg
    20761 atgttgtcat ccacactcac cgttacgaat ggtgtatcaa tcactccgtt ttggtaaata
    20821 ccctcacggt gttcttgttt tccttccagc aattggaagg ttgtccaaga aagcggcgct
    20881 aggtgaactg gaatggtcac gcgcacttga cgagcaatac gagcttggcg gaacttgtct
    20941 tttggtaaat tatactcaaa attagctccg aggtcttcga ttgtagcctc tacaggacga
    21001 ccatccaagt cctccacacg gtaacttggc aagataagag cagccatctt tttgtagcct
    21061 tctgttgggt gcaattcctt gaaatcacaa gtcgccacat caatcactgt gctgacagta
    21121 tcgaccttat catgcaagcc tgtgttaatg acagtaaaga gatagtcact ttgagcctta
    21181 tccgtagcaa ttttaccctt ccactcgttg agcaagttac ttttaacaaa gtttcctact
    21241 tggttgacct tggcaaaacg cgtttccatc tcgcggtgaa cttcgtccac gctacagcca
    21301 cagatactat catgtggcgc attctgcaaa agtgttttcc aagcataggt caactggtcc
    21361 ttgtggttgt gtccaccagt gataatagtc aagggttcta ccacttgctc taggaggttg
    21421 ctattttctt ggaaggcttg ttttaggtaa atgcgggatg aagaagtgtt ggcaagtgtg
    21481 taccagccat ctgtttcctg actggtcaac tcgcctgtaa cagttgataa gtgttcagga
    21541 agcgcacctt ctacagcttg aacatattca tcaaaagaac tatgaacaaa gattacatcc
    21601 gggaagagtt catttgccac acgaatggct tcgctcagat ttttctgtac aggctggtgg
    21661 tcacagccgt tcatcatcaa ccattggttg gtcgaagcgt aggcacgcac atctgacaat
    21721 ttttgtttcc agaaggtcaa ggcctcatct ttgtcaactg gaatttcatt cccgttactg
    21781 taccagttgg caaagagaat acctaaaaca cgactaccat ccacaccctg ccagtacatt
    21841 tctgaaaact gagacgtaaa ctgctcatct tcaaggactt ggttgtcaaa tccaatcggc
    21901 ttcacaccac gaccaaaggc cgccacgtga atgcctgatt tttgaagaat ttgaggcgct
    21961 tgtcccatat ttccaaaggt atctggaaag tagccaatct gggttgattt accccatttg
    22021 gcagcttctt gttgaccaat caaggtattg cggacattgg cttcactgga gatcaagtag
    22081 tcatcctgca agatgtaaaa gggaccaatt ttaagtttgc cctcgtcaat gtagcgttgg
    22141 accttgtcgc gattttcagg gcgaatttgt aagtagtcat caaggacaat agtttgtcca
    22201 tccaagtgga aactcttgaa ctcagggtca ttttcaaaga gatcaaagag attgtcaaac
    22261 aattccacca actgcatacg atggctttca aaaggcaagt accactcacg atcccagtga
    22321 ctatgtgaga taatatgtac aacaacattt tccatgaagt aaaacctcat tctaaattta
    22381 aattttaacg ttttaaatgt aaacttgtga ttctgataag aatcggttga gctaaagcga
    22441 gccccttagc gaatatccaa gtaatccaag accaactcac agaacatcat gttagcccag
    22501 gagaaccatt cacgagagta gagggtcgga tcatctacat gaaagctttc gtgcatgaca
    22561 cctgtaccac catcgcaggc aaccagctga tccagcaaga attttttctc tgccttatct
    22621 cttgttgtca agccttggat agaaagggcg attggccaga tatagcgata gaaggtatga
    22681 gaactgccga gaccgcttgc gtattctcct tggtagaagt atggattttc agagctcaaa
    22741 atggtacgac gagtagcttg atacacttca tcatcgaccg aacagtagcc cagatagggc
    22801 gcagctagta gacttggtac atttggatca tccatgatgc tggcatttcc taggccatcc
    22861 acttcaaaag cgtaaatctt ttcgcccttg ctgttggtgg tgtaagcgta gtttttgatt
    22921 ccttcttgga tttcatcctg aagacgcttg gcatcagcaa taacactctg gctatcagct
    22981 aggtttaatg ctgcgaagat ttcttgcaca taacccaaga ctactacagc aaacatattt
    23041 gacggaatca agtaactata ctggcaacag tcatcactcg gacgaaaagc tgaccaagtc
    23101 atacctgtca ctgcaaagtc aggtccaaag ccatcattta ccaaggtgtc ttccttacgg
    23161 tccgtatctc ggacaaaacg ataaggagag ttcttgtggt cttgttccac cgtccacaga
    23221 tggagaattt ccttagtcgc tgcgacaaaa atctcatcaa actgactagt ctcgccagtc
    23281 tctttccaga ggagataagc caactgcaaa ggatagcaaa gcgaatccac ctcatacttg
    23341 cgctcccaga tccagccgtt aaggtctgtg tggtcagtct cgtggtgccc tttccagttc
    23401 tcctcaatgt tgaaggagtt agcataggga tccttgagta ccaaggtcat ctgacgtttg
    23461 accaaacctg caatggtctg acgcaggagg gcatctcttt tagctacatg aaggtagggt
    23521 ctgagttggg ctgtcgaatc tcgaagccac atggcaggaa tatccccagt caagacaaaa
    23581 gttgaaccat cttctaagat ttcaaccgta ttgtccaagg tatcggtgta gcaacgctcg
    23641 aaaacatcca cccactctgg atagtcctta gcccgctctg ctacttcatc tagccactct
    23701 ctgacaattt ctttcgaata aaccatttta aattttacct ctttcaaaca agtttcatgt
    23761 tcagtatata gcttttgaga cagaaaatac atacacaaat gatagtcatt tttctacaaa
    23821 atagttattt ttgaaactcc ttttctaaag gccctctttt tctgatataa tgtagaaaaa
    23881 cagctaaagg aaagactatg aaaccactac ttgaaaccat cgatacccgc tttggaacta
    23941 ccaacaagca tgccttttct cggggaaata ccctgccata cacaggcgtg ccttttggga
    24001 tgaattactt tgtgccccag accagtgacc aggatggatc ttggtttttt gatccacatc
    24061 tgcctatctt tcaggggatt cgattaactc accagcccag tccttggatt ggggactact
    24121 cttggctcct tctgacacct gtcaccagtc aactgggggg agacagcctc tttcaccgcc
    24181 agtcttctta tgatatagat aaagcttgct tccagcctca ctatttgaag ctcttttctc
    24241 tgcgttatca gattgaaacc cagctcacac cgacttgcta tggtgcttct attcgtttga
    24301 atcaaaagca aggcaaagcc ctctccctct atcttcacgc agcagatgaa ctgacagtag
    24361 agcaagtaga taagcggact cttgccctgc gacaagaagg taaaactgaa actaacaaaa
    24421 attcgctaac gatgttcact gccctgcaaa tgaacacgga tattcttgct atcagccaag
    24481 aagctggaga ctggcgaatt gacttagcaa gtagtcaaac cgagatgcaa ctagccactt
    24541 ctttcatctc tccttctcaa gctctaatca atctacctca agaagatttt gatagctgta
    24601 aatcaagtgc ccaagtggat tgggaaaatc tcctccatcg ttttgacatt atagagacag
    24661 gagaggctga ccgaaccttc tttgaccact gcctctacag actcttcctc ttcccacaga
    24721 ctttttatga gattaatgaa tcagggcaag ccatccacat ggatctggct actggtactg
    24781 tcaagcctgg tgtcctcttt agcaacaatg gtttctggga taccttccgc accaccttcc
    24841 ccctctttgc ccttatcata ccggagcact atcaacgctt tttagaaggt ttcctcaata
    24901 gctaccgcga tactggtttc cttccaaaat ggctggctcc agatgaacgg ggtatgatgc
    24961 caggtacact tttagacggt attatcgcag atagcgcctg caaggacatg acccccgacc
    25021 tagaaggaga actcttccaa gccatgctcg aaacagccag caaagccgac cctctcggca
    25081 tcaatggccg ccacggacta gcccaatacc aagaactagg ttacctctct accgaccacc
    25141 acgaaagtgt tagccatact ctagactatg cctatagtga cttttgtatc gccagctgtg
    25201 ccaaaaaact agagaacata gaaatcgctg aaacctacaa ggctgcttca caaaattacc
    25261 gccagctatt tgacgctgag acaggttaca tgcgagcacg agacaatcaa ggaaactttc
    25321 accctgactt ctctccttat agttgggggc gagactacgc tgaatgctct gccattcaag
    25381 ctactttagg cgttctccac gacatccctg gcttaatcca actgatgggt ggaaaagaaa
    25441 cctttagcaa ctatcttttg aaagcctgtc aagatgctcc cctctttgaa acaacaggct
    25501 atggttacga aattcacgaa atgagcgaga tggctactgc tccttttgga caaatagcca
    25561 tttccaacca gcctagtttc cacattcctt atctcttccg ttacagcgat taccctgact
    25621 acactgccct tcttatcaag acgctccgtc agaaagcttt tcacccaagt tgggaagcct
    25681 atcctggaga tgaagacaat ggtagtctct ctgcttggta catctggtca gctctcggat
    25741 tttatccaac ctgtccagga aaaccaagct atgacctcgg aatccctctc tttgaccatc
    25801 tacgagtcta cctcgctaaa gaagataaat ggctggatat ccatactaaa caaaaccaca
    25861 accattttaa ctttgtcaaa gaatgccgac tggacaaaac actcgtatca actattcaac
    25921 accaagacct cttaaaagct gaacaactaa ctttcaccct cagctggtta ccaagtcact
    25981 aataaccaaa agaacctttg catcgcgcaa aggttcttct tttatgaaac ttggacttga
    26041 agttgatcca caagctgacc atccaccgtt aggttcagat agaaatccaa ggttttatct
    26101 cctgcgaaat acaaggttgg tagcgtcaga cttttttcag tctcaccagt ttgaaacgca
    26161 aggacttgaa tctcatcctg ataggcgaca ccatggacac ctgtccccgg ttgaatcgaa
    26221 atcttagctt ctaaaggacc actacattct ctgcgcttaa ctgtaaaata ggcattttct
    26281 ccctttgcca cagctaggct tttttctgca aatgctagtt cctgaacaac ttcttttttt
    26341 gataatccag gagttttata aagggaaatc ttggtcaaca aaggcaaagc ctgtgattct
    26401 gtaatgacta cacgtatctt ctgtgcctca acaactgctc ctcgtaagag acgtttgtaa
    26461 ccaacagtat gacccgaacc aaactcctgc cagacaccat ccacctctac ttgcacatga
    26521 aaagcagcga ttcgttgccc tagcttcaaa tcttctctta actcaattac atcaaaagtt
    26581 ttaggagaac ctaagtcgag ttctaactgg atgggcaagt ctgcatcgct tgcccaagag
    26641 ctggtctcaa ggccgtctgt caaatggcga caagcaaagt ctgcggaaag agctggacca
    26701 gatacctcag ctcccagagc caaatcttct ttatagagct cattgcgata ggtcgcaaat
    26761 tcataaagtc gttcaatatc ctttgcatca aagagcccag cttgattcgg cggaatatta
    26821 agcaagagtg gagttcctcg ccctactgag tgaaagtaga tttcgaccaa ctcctcgaga
    26881 gacttaggat cctgatcctc atggtagaac cagcctggac ggatggaaac atctgcctct
    26941 ccgattgaaa aaatcgtgcc cgagggatcc ccgtgctgaa gatagttcag ctctgcttct
    27001 gttcctagtt tatcaggatt caccttttgc cacagtggat cacctgcata ccctcgttca
    27061 ttgccaatcc agcggatact ggtgccttct gttgaaaaaa tcaagcaatc gccctgcagg
    27121 tcacgaatgg tttcaaacca tttttcaaat tcataattaa ccttttgcgc gccctctcct
    27181 ctggcaccat ccatccaaac ctcagcgaac ttaccagcat tcccatagtt aggatttgat
    27241 aagatttcct tcaactgagc cagataatag gcattgtagt ccgcttctcg gtccacatga
    27301 tagaggggac tatgggcatc ccacggtgac aggtagaccc ccatatccat atcaaactct
    27361 gtggcagctt gggatacttc aaggagcaag tcgccctttc ctctcctcca aggactgacc
    27421 ttaaccgaat aatctgtgtg agctgtcgga taaaggacaa agccatcgtg gtgcttgacc
    27481 accaaaatca actttttgaa gcccgtttcc ttgagcacac gaacccactc acgcgcatcc
    27541 aactgactcg ggttaaagcg ctcaggatcc tcctgtccag tcccccattc ttggtcataa
    27601 aaggtattag gaccgaagtg gataaaagct gctagttcat ctcccagata agctagctga
    27661 gtctgacttg gtaacggtcc atgcggtttg attttcttca tcgttatata tctccttctt
    27721 tagcaacttc tcttattcac aaagttatcc acaggttgtg gataataaac catgatctaa
    27781 cagtcagctc tttccaactc caactggtag gctcaggatt tttctcctca atattccctc
    27841 ttcaaacagc tctgtcatat ggttctgcta acacctctca gaatgctact ctccataatt
    27901 attctcaggc ttctttcagg tcattgtgtg aagaccattc actatccaga cttttccaca
    27961 gattgtgaat aagtgattat tctaaactat cacctcgtcg actataacaa aaaataaaaa
    28021 gagggtcaat aagataatta tctcttccta aatactcaga aaagattcta taatttgtac
    28081 aattgaacaa gaaatgcttc tccaaaatct atcttcaaaa gaatgttgca tctttttatc
    28141 ctttcacaca aacatttctc tttatacaga tccaagtgat aaccagatga taattggaca
    28201 ctttttgatc atttgtatat tttttgaaaa attttttaaa aaaattttat atccttttta
    28261 tagaagttta tgcatttgta tctatgtatt ctatcctatg ttatgtataa taacataaaa
    28321 ttgtgctaaa catcataatt tatattattt tccgagaaat tatagccatt gacattacct
    28381 aggaattgcg ttataacata catgtaagcg gtacccaaaa tggtgccaag tcaaaatttt
    28441 taaggaggaa aatacatgtc ttcacatcca attcaggtct tctcagaaat tgggaaactg
    28501 aaaaaagtta tgttgcaccg tccaggcaag gagttagaaa acttgttgcc ggactatctt
    28561 gaaaggcttc tttttgatga tattcctttc ttggaagatg ctcaaaaaga acatgatgca
    28621 tttgcccaag ctcttcgcga tgaaggaatt gaggttctct acctagaaca actcgctgct
    28681 gaatcattga cctctccaga aatccgcgat caatttatcg aggaatactt agacgaagcc
    28741 aacatccgtg atcgtcaaac caaggttgct attcgtgaat tgcttcacgg catcaaggac
    28801 aaccaagaat tggttgaaaa aacaatggct gggattcaaa aagttgaatt gccagaaatt
    28861 cctgacgaag ctaaagatct aactgactta gttgaatcag agtatccatt tgcaattgac
    28921 ccgatgccaa acctctattt cactcgcgac ccatttgcaa caattggaaa cgccgtatcg
    28981 cttaaccaca tgtttgcaga cactcgtaac cgtgaaacac tctacggtaa gtatatcttc
    29041 aaataccacc caatctatgg cggaaaagtg gatttggtct acaaccgtga agaagatacg
    29101 cgtatcgaag gtggagacga gttagttctt tctaaagacg tccttgcagt aggtatctct
    29161 caacgtacag acgcagcttc tatcgaaaaa cttttggtca acatcttcaa gaaaaatgtt
    29221 ggcttcaaga aagttttggc ctttgaattt gctaacaacc gtaaattcat gcacttggat
    29281 actgtcttca ctatggtaga ctatgacaag ttcactattc acccagaaat cgaaggcgac
    29341 cttcacgttt actcagttac ttacgaaaac gaaaaactta aaatcgttga agagaaaggt
    29401 gacttagctg aacttcttgc tcaaaacctt ggtgtagaaa aagttcattt gattcgttgc
    29461 ggtggtggca atatcgtagc agctgcgcgt gaacaatgga atgacggttc taacactttg
    29521 accatcgcac ctggtgtggt agttgtttat gaccgcaata ccgtgaccaa taagattttg
    29581 gaagaatacg ggcttcgctt gattaagatt cgcggaagtg aattggttcg gggccgtggt
    29641 ggacctcgtt gtatgtctat gccatttgaa cgtgaagaag tgtaatcgct gttcgatatt
    29701 cgtcaataga aaatgtaaaa aatagaaaga ggaaataata aaatgacaaa ttcagtattc
    29761 caaggacgca gcttcttagc agaaaaagac tttacccgtg cagagttaga ataccttatt
    29821 ggtctttcag ctcacttgaa agatttgaaa aaacgcaata ttcaacacca ctaccttgct
    29881 ggcaagaata tcgctctcct atttgaaaaa acatctactc gtactcgtgc agcctttaca
    29941 actgcggcta tcgaccttgg tgctcaccca gaatacctcg gagcaaatga tattcagttg
    30001 ggtaaaaaag aatctactga agatactgct aaagtattgg gacgtatgtt tgacgggatt
    30061 gaattccgcg gattcagcca acgtatggtt gaagaattgg cagaattctc aggcgttcca
    30121 gtatggaacg gtctaactga cgaatggcac ccaactcaaa tgctcgctga ctacttgact
    30181 gttcaagaaa acttcggtcg cttggaaggc ttgacattgg tatactgtgg tgatggacgt
    30241 aacaacgttg ccaacagctt gctcgtaaca ggtgctatcc ttggtgtcaa tgttcacatc
    30301 ttctcaccaa aagaactctt cccagaaaaa gaaatcgttg aattggcaga aggatttgct
    30361 aaagaaagtg gcgcacatgt tctcatcact gaagatgctg atgaagcagt taaagatgca
    30421 gacgttcttt acacagacgt ttgggtatca atgggtgaag aagacaaatt cgcagaacgt
    30481 gtagctcttc ttaaacctta ccaagtcaat atggacttag ttaaaaaagc aggcaatgaa
    30541 aacttgatct tcctacactg cttgccagca ttccacgata ctcacactgt ttatggtaaa
    30601 gacgttgctg aaaaatttgg tgtagaagaa atggaagtaa cagacgaagt cttccgcagc
    30661 aagtacgctc gccacttcga tcaagcagaa aaccgtatgc acactatcaa agctgttatg
    30721 gctgctacac ttggtaacct ttatattcct aaagtataat tttagataat aaaccgtcta
    30781 ccaacagcta tgagggctgc gactaatagc tttagtccgg tcctctttta tgtaatggta
    30841 atctattatt tcttataaaa tatgtgaaaa atcattaaat tgaaatctaa acgcattcta
    30901 ttgagtgtga taaaggagaa tttatggcaa atcgtaaaat tgtagtagct ttgggaggaa
    30961 atgcgattct ttcttctgac ccatcagcaa aggctcaaca agaagcttta gttgaaacag
    31021 ctaagcatct tgtaaaattg attaaaaatg gagatgatct gattatcact cacggtaatg
    31081 gacctcaagt tgggaatctc ttgctccaac atttggcatc agactctgaa aagaaccctg
    31141 ccttcccact cgactcactt gtcgctatga cagaaggtag catcggtttc tggttgaaaa
    31201 atgctttgca aaatgctctc ttggatgaag gcatcgaaaa aaatgttgcc tctgttgtaa
    31261 cgcaagttgt cgtagataaa aatgatccag cttttgttaa cttgagtaaa ccaatcggtc
    31321 ctttctattc agaagaagaa gcaaaagcag aagccgaaaa aagcggagcg actttcaagg
    31381 aagatgctgg ccgtggctgg cgtaaggtcg ttgcctcacc aaaacctgtt gacatcaaag
    31441 aaattgaaac catccgtact cttttaaata atggtcaagt cgtcgtagct gcaggtggtg
    31501 gcggtattcc cgtcgtcaaa gaaaacaatg gacatttgac tggtgtcgaa gcggttattg
    31561 ataaagactt cgcttcccaa cgtttggcag aattggttga tgcagacctc ttcatcgttt
    31621 tgacaggtgt agattatgta tttgttaact acaacaagcc aaaccaggaa aaattggaac
    31681 atgtgaatgt tgcccagctg gaagaatata tcaaacaaga tcagtttgca ccaggtagca
    31741 tgcttccaaa agtagaagca gctatcgctt ttgtcaatgg tcgtccagaa ggaaaagcag
    31801 ttattacttc ccttgaaaat ctaggcgcct tgattgaatc tgaaagcgga acaattattg
    31861 aaaaaggata agttgtttta ctaataagat gtattctatt tctagtatct ttatatcaaa
    31921 ttagaaatta ttcttgaaaa catgtacaat atttcaaaag atactagttt tagactttaa
    31981 tatggtaaaa caaatataaa tagaaagcgt tttcttgaat gtttatttaa gaaagtagtt
    32041 ggttttttac actttgttag acatcaggag gaaaaacaaa tgagtgaaaa agctaaaaaa
    32101 gggtttaaga tgccttcatc ttacaccgta ttattgataa tcattgctat tatggcagtg
    32161 ctaacttggt ttatccctgc gggggccttt atagaaggta tttacgagac tcagcctcaa
    32221 aatccacaag ggatttggga tgtcctcatg gcaccgattc gggctatgct aggtactcat
    32281 ccagaggaag gttcgctcat taaagaaacg agcgcagcga ttgatgtagc cttcttcatc
    32341 cttatggttg gtggtttcct tggcattgtc aacaaaactg gtgctcttga cgtagggatt
    32401 gcctctatcg tgaagaagta taagggccgc gaaaaaatgt taattttggt actgatgcct
    32461 ttgtttgccc tcggtggtac aacttatggt atgggtgaag aaacaatggc cttctatcca
    32521 ctccttgtgc cagttatgat ggccgttggt tttgatagcc tgactggtgt tgcaattatt
    32581 ttgctcggtt ctcaaatcgg ctgtttggca tctactctga atccatttgc gacaggtatt
    32641 gcttcagcga ctgcgggagt tggtacaggg gacggtatcg tacttcgtct gatcttctgg
    32701 gttaccttga ctgctcttag tacttggttt gtttaccgtt atgcggataa gattcaaaaa
    32761 gatccgacta agtcactggt ttatagtact cgcaaagaag atttgaaaca ctttaacgta
    32821 gaagaatctt catctgtaga atctacactt agcagcaaac aaaaatcagt tctcttctta
    32881 tttgtgttga cattcatctt gatggtattg agcttcattc catggacaga ccttggcgtt
    32941 accatttttg atgactttaa tacttggttg actggtcttc cagttattgg taatattgtc
    33001 ggttcatcta cttctgcact aggtacttgg tacttcccag aaggcgcaat gctctttgcc
    33061 tttatgggta tcctgattgg tgttatttat ggtcttaaag aagataagat tatctcttcc
    33121 ttcatgaatg gtgctgctga cttgctcagt gttgccttga tcgtagcgat tgctcgtggt
    33181 attcaagtta tcatgaacga cggtatgatt accgatacaa tcctcaactg gggtaaagaa
    33241 ggcttgagcg gtctatcttc acaagtcttt atcgttgtaa cttatatctt ctatctacct
    33301 atgtcattct tgatcccatc ttcatctggt cttgccagcg caactatggg tatcatggct
    33361 ccacttggag aatttgtaaa tgtccgtcct agcttgatta tcactgctta ccaatctgct
    33421 tcaggtgtct tgaacttgat tgcaccaaca tctggtattg tgatgggagc tcttgcactt
    33481 ggacgtatca acattggtac ttggtggaaa ttcatgggca aactcgtagt cgctattatt
    33541 gtagtgacca tcgcccttct tctccttgga accttccttc cattcctata aaatagtgag
    33601 tgaggtgatt ccatgaaaat agatataaca aatcaagtta aagatgaatt tcttatatca
    33661 ttaaaaacct tgatttccta tccttcagta ctcaatgaag gagaaaatgg aacacctttt
    33721 ggacaagcaa tccaagatgt cctagaaaaa actttagaga tttgtcgaga cataggtttc
    33781 actacctatc ttgaccctaa aggttattac ggatatgcag aaatcggtca gggagcagag
    33841 cttctggcca ttctctgtca tttggatgtt gttccatcag gtgatgaagc agattggcag
    33901 acaccgccat ttgaagcaac tatcaaagac ggctgggtat tcggacgtgg tgtccaagat
    33961 gataaaggcc cttcgctcgc agctctctat gcagtaaaaa gcttgctgga ccaaggtatt
    34021 cagttcaaaa agcgcgtacg ctttatcttt ggtaccgatg aggaaaccct ctggcgctgc
    34081 atggcacgct acaataccat cgaagaacag gccagtatgg gctttgcacc tgactcatct
    34141 tttcctctga cctatgctga aaaagggctt ctacaggtca aacttcatgg ccctggatcg
    34201 gatcaactag agcttgaagt aggaggcgcc tttaacgttg taccagacaa ggccaactac
    34261 caaggtctcc tctatgaaca ggtttgtaac ggtctcaaag aagctggtta tgattaccaa
    34321 accactgaac aaaccgtaac ggttctcgga gtgccaaagc atgctaagga tgctagtcaa
    34381 ggtatcaatg ctgtcatccg actagctacc attcttgctc ctctccaaga acaccctgct
    34441 ctcagttttc ttgcaacaca agcaggtcaa gacggcacag gaagacaaat ctttggtgat
    34501 atagcagatg aaccttctgg tcacctatcc tttaatgtcg caggtctcat gatcaatcat
    34561 gaacgttctg aaatccgtat tgacattcgg actcctgtct tagctgacaa ggaagaacta
    34621 gtagagttgc ttacaagatg tgcacaaaac taccaactcc gctacgaaga gtttgactat
    34681 ctagcgcctc tatacgtcgc agaagacagt aaactcgtta gcacactgat gcaaatctac
    34741 caagaaaaga ctggcgataa cagtcctgct atttcatccg gtggtgccac ttttgctcgc
    34801 accatgccaa attgtgtagc cttcggcgcc ttattcccag gagcgaagca gacagaacat
    34861 caggcaaatg aatgtgccgt tctagaagat ttgtaccgtg ctatggatat ttatgccgaa
    34921 gccgtctatc gacttgcaac ttaatcaggc aactgtttct accaaaaaaa atcgaccgat
    34981 taatgaactg caccccaaaa gttagacaga ataaatctaa cttttggggt gttttattat
    35041 gaaattgagt tatgaagata aagttcagat ctatgaacta agaaagcaag gacaaagctt
    35101 caaacagctt tcaaaaagat ttggtgtgga tgtttctggt ctaaagtcat ctgaatcttt
    35161 gagatgagct ttataaatcg cttttttcag tttttgcact ggtgtttcga taaactcaaa
    35221 ctttttagcc gtggtattgc ctgattttat agtatattga aactagaata gtacacctct
    35281 ccttctaaaa catttttaga aatcgatttg actgtcctga tcgatttgtt ctgttcttat
    35341 ttcattttac tatatttgag ccacttcgtc tttaacggct ttattcataa gctcttgtaa
    35401 tttttcttta ctatcaatta cttctgattt tccgttgtaa tttattgtaa taggttttaa
    35461 cttacctaat ttctcgacac gctcattaat ttgatctttt ttgaaggctg cttatgtttt
    35521 tcctaagatt ttttcaaaaa tatatttatc agatagcggt ttgtcttctt cttcagcttg
    35581 gtttttgtat taatttgaaa cataaggaac aaatccttca tagtaaccta atgctcccat
    35641 aagttcaaaa gcttgttttc taattcaaac cattgcaact cagatttcag cttttcagat
    35701 aaatcctgct catccaaata atgacttgaa attagtgctg aactcgtttc tgtatcctgt
    35761 acaggctgag cacccatacc agcaaaaaat aaactcgttc ctagcaagac cgaacaagct
    35821 cctattgcat atggcctcaa agaaaaacgc tgctttctct caaattgaaa ttctttcatc
    35881 ccatctccca tcattcatta ttactgtata ttttgtatat cagaaatagt ttgtattcac
    35941 aaatctttct agttattccc ttatcattcc taattaaggg agataacata caataatttt
    36001 tagttaaatg tatatcgatg ttttttgttt ttcttaataa acgcaataca aaaagagcct
    36061 gttaccaagc tctttgtact caatgaaaat caaagagcaa attaggaaac tagccacagg
    36121 ttgctcaaaa caccgttttg aggttgcaga tagaactgac gaagtcagct caaaacactg
    36181 ttttgaggtt gcagatagaa ctgacgaagt cagtaacatc tatacggcaa ggcgacgctg
    36241 acgtggtttg aagagatttt cgaagagtat tagtctatta tttcttctca gcgcgaaggg
    36301 ctgacaagat ttgtgttcgg atatcatcca caccatttgg agtatttggt aaaaagatag
    36361 tttgatttcc tttagaggca aaggtattca aggtatccaa atactggttg gtcaagagga
    36421 tagacatgat ttgttcttct gtcatgccaa cattggcttc cttgagttcg gtgatagact
    36481 ctgccaatcc atccacaatc gccttacgtt gttgggcaat ccccacacca tgaaggcggt
    36541 ctttttctgc ttctgcttca gctgcagtga caattttaat cttgtcagct tccgccaatt
    36601 cttgtgctgc gacccgctta cgttgcgccg cattgatttc attcatggat tgcttaactt
    36661 ctgcatctgg ttcgaccttg gtaatcaagg ttttcacgat aatgtagccg taagtggtca
    36721 tttcttctgc tacttggtgt tgaacttcaa gggcaatctc atcttttttc tcaaacaatt
    36781 catccaaggt taattttgga acagaagagc gaagagcatc ttcgatataa gatttaatct
    36841 gagattctgg acgtatgagt ttatagtaag catctgtcac gctctgctcg ttgacacggt
    36901 actgagtcgc tacattcatc ataacgaaca cattgtcctt ggtcttagtc tcaaccacaa
    36961 tatcactttg caacaagcgc aactgaatcc gtgctgcaat cgagtcaatc ccaaaaggca
    37021 agcgaatatg aataccgcta ttagcaacct tttggtattt cccaaagcgt tcaataatcg
    37081 ccaccgactg ctgacgaacc acataaactg tactcagtgt gactatcacc aataggagca
    37141 cacaaacaat cagaaaaatc atgaaaaata ttgccataat ggaacctcca caagtatttt
    37201 tctagtatta tagcacattt aaagaaggct gtgccgtttt tactgcgatt tttcctgaaa
    37261 tgtcaataat tagaggtgaa ttgtcctatt gtcgtccaat ctcttgctaa aataactctt
    37321 tataaaaggc aatcgtttct tctaaggttg gcataaatgg atttcctggt gcgcaggcat
    37381 caatcaaggc attcttagaa aggtattcaa agtcgaaatc tttttcttca ataccaagtt
    37441 cagtcagttt cttaggaata cctactgtct cagaaagctt ctcaatctca gcaatcgcat
    37501 aatcggcaca ttcttgatct gatttacctt ctacatgaag tcccaaggct ttggcaacat
    37561 tgcggaaagc ttctggtaca cgtttagcat tttcacgttc tataactggt agcaacatgg
    37621 cacagcacac gccatgtggc aaattatata ctgcaccgag ttggtgagcc attgagtgaa
    37681 catagccaag accagcatta ttaaagctca taccaccaag gaagatggca ttgaccatac
    37741 cttcacgcgc ttcaatatca tgtccatttt ctacagcacg aggaagatat tccttgataa
    37801 gctcaatagc accaatagac agtttcttgg ttacatcata agcaccaggt gttaccaaag
    37861 cttcaacagc atgagtcaga gcatccatac cagtagcagc agtcaggcct ttaggtttgg
    37921 aaagcatgag ttcagggtca ttaacagaga taagagcaag gctattcttg tcaaccatta
    37981 ccatcttaac cttgcgttct tcatcagtaa tcacatagtt aatggtaatt tctgctgatg
    38041 tacctgccgt tgtattgata gccacaactg gcaagccttt tttagcagac ttatgaagcc
    38101 cttcataatc ctgtggtttt ccaccatttg tagccatgat agaaatacaa ctagctgcat
    38161 cctgtggaga acccccgcca agactgatga taaagtcaca gccatgttct tgcaaagcag
    38221 ctaccccatc tgtgacattt ttacaagtag ggtttggctc tacatcgcta aagatgacat
    38281 attcgattcc ttctgtatcc agtggtttta aagtcttagg taaaatatca ctgccttcga
    38341 tgtacttatc tgtcaccaaa agtgcctttt tatagccaag ttccttgata taaggaccca
    38401 cttcatttac aacacctttt ccaataaggt tgactgccgg aacataaaat atagccatat
    38461 aattttcctc ctgcgcctcc gcgctatcta tttaacatca ttatattact ttttgtattc
    38521 gttttcaatt atttgtgaat tgtttaacaa atctttttaa aaggatagga gggacgtaag
    38581 ttcacctcct atataaacaa gttttattta tgtagtggcc ccaacaactg acatgcacga
    38641 tagtctgctg attctagatc ttctgttcca aataaggacc aatttttagg tctaaagata
    38701 tcttcctcag gtacattatg catattgaca ggaattctca acatagaagc caaggtaatc
    38761 aagtctgctc caatgtgtcc atatgttatg gctccgtgat tagctcccca attattcatg
    38821 acgtcataga cagacttgaa agcacctttt cctgtcaaac gtggagcaaa ccaagtagtt
    38881 ggccatcctg gatctgtacg attatctaaa gtatggtgaa catcttcagg aagttcaagt
    38941 gtgtaacctt ctgcaatttg tagcactgga ccaacccctt ttagaagatt gagacgtacc
    39001 attgttactg gcatatcccc cttcgtcaag aaacgagttg agaatcctcc tccacggaag
    39061 tattcgcggt ttgctggtgg gaagtctgta ttttcaagca tagcctgcac ttcactttct
    39121 tccaactccc agaatggttt cataataggt ttgccatctc gagtagcttg acctgtacca
    39181 tccaatgtac aagaaccaga gttgattaga tgtaagaagc cagctgcagc acgaccctct
    39241 aaagtatgtc ccgttacacg tttaacagcc tctgggctcc aataagtacg cacatcagca
    39301 aagatttgtg gagtatttgt taatagataa ttaaagagca tagacacacc atttagtgaa
    39361 tcattctctg tcgcaaatac aaatggtttt cgaataccat tccagtcaaa ctgagtattg
    39421 aggaaagttt ccataaagtc cccatttgga aaatggtctg tccactgacg ttgaccttgg
    39481 aaaccagcta ctaaagcatg gtgaccaacc gcttcttcct caaaaccaag ttcagcaagt
    39541 cttgggttac caaccattaa gtcacgtcca atcatgaaca tcttaataac aaattcccat
    39601 tgtctatctt tttcttcacg gcttaaaaca aggtcttcac ggttatggtc gaatccttct
    39661 tttacgtttt ctttcaccca tttgagcgca cgttcgaact cttcagggtc gtaaatacca
    39721 cggtccatac ggcgcgtgaa ctccgtcata tctaccgatt catttcgcat tcctaagtat
    39781 tcttggaaga aatccggatt tacaatagaa ccaccaatcc ccatcgaaac actacccatt
    39841 gataggtaag cagtgtctct catcaagcca gttgcaagag ctgcccgcgc ataacgtaaa
    39901 agtttttctt tgacatcttc tggaatagct gtatcattag cttcctgaac atctctacca
    39961 taaatcccaa aggctggaat ccctttttga gtatgtgaag ctagtacagc tgcaagatag
    40021 acagctcctg ggcgttctgt cccattaaat ccccaaatag catgaggaat atctggagac
    40081 atatccatag tttcactacc ataacaccag catggtgtaa ctgtaattgt tgcgcaaaca
    40141 tttgattttt taaacaactc atgggaagct gcagcctctg gaacacgacc aatggtagat
    40201 ggagaaatca cacattccac aggttcccca tctggatatt tcaatgtgct tgaaatcaaa
    40261 tctgccacac ttttagccat gttcattgtt tgtacttcaa gtgattcgcg tacaccttga
    40321 cgacgaccat caatagtcgg acgaatccca atacgtggat gttggatcat aatactttcc
    40381 tcttattttt ttgtttcttt tatactacca tcgccattat atttacggta gcctgggtga
    40441 cgacctgtaa ccttataatt ttctcgcatt gagaatagac gttctaaagt cggacgagca
    40501 atttcttgct cctcaatgcc ctttgtagaa agtaacattc ttccgtggaa ggttgtcttt
    40561 gcgactaatt ctaaagtttc catacggtag tatgctgtaa tgacatcgct tccgacagtc
    40621 agagctccat gattttctaa tagcatgaca tcatgatcgg gcagataagg tgtaattgct
    40681 tctggcactt ccattgtaga cggtactcca aatggggtaa taggaattgc cccaaccaca
    40741 atcgcgctct caattagtga ataagtatct aaaggaatgt gtgcaagagc aaatcctgtt
    40801 gcaatcggtg gatgcgcgtg aacaactgaa cgaacatcct cacgttcttc gtagcaccga
    40861 atgtgcattt taatttcact agaaggacag taatcacctt ctgcttctaa aatctctcct
    40921 ttaagattta acttcaccag cttttctggt gtaataaaac ttttgctgat accagtaggt
    40981 gttgcaagaa tggtatcctc atctaatcga acagatacat tcccatcgtt agcagcaacc
    41041 caaccaagtt gccacatctt atgacaaaca tcacaaatct gttcacgaat taatttttca
    41101 tcttgagaca taaactcact cctttctaaa aatgaagttc gaattcaaga tttccgttac
    41161 tgttaatggt aatagtaacc atatgatttt tctgatcaaa attgagagat ggctgatcat
    41221 aggaattatt tgttcctgat aatgaatgaa tagtaggaag tttatctata cctcttaata
    41281 ctatctttgt aacccttttc tctccaaact gagtatcttt gatataatgt tcttcaatcc
    41341 acttaatcgc acctttcttg gtcaattgtg gtagtttctt tgcctgattt gcatcttgag
    41401 cattagacca tagtgaatct ttatttgttc taaaattatt taaattcact ttcaatccat
    41461 tacttattct ctcaaaaata ccataggtat gaggttcaaa ttcaacatcc atttctaaag
    41521 aattaaattt taatttccca gtctgtttaa cattctcatt taccttataa ttatagacaa
    41581 accatctatt atctaatttc tgagcaaaga tatctccttc ataattcata ggatacagct
    41641 tatttaagta ttcttttcta gcctgagtag agctaaattc tggagaagtg atttccttaa
    41701 tctgaatacg gctaccagaa acagactctt tcaatttatc tgtttttaaa acacctggaa
    41761 tagctggaat aacattataa cgaccagtca tgtataatgg agtttgttct ctatcagtat
    41821 ttacgtttac aaagaactta ccatttcctt tatttgaata gtctccatgt atgaatactt
    41881 ttgtatcctc aagtaccttt tctttggatg gtgctggatg cgctattaca tatctaaaaa
    41941 attcttttat tacttcacta aagagtaagc tttctttatt atttacacca taggtataag
    42001 ctggatgttc aaaattataa actacacctc cattaaggta aatccctaaa gcttcttctc
    42061 ctagcataga ctctggttca gtcaaccatt gtctatctcc ttgacttttt ccgatattac
    42121 cacttgcaaa cagtttccat ttacctgtct cataccactt ccaagtatcc atcaaaccac
    42181 cccattgata agtatgattt gaaagccaaa gtcccttcat ataactttct gtagttgaat
    42241 catttccctc agcagcaggt gtatttttga acatgaaaat aaggttcttc cagtacttat
    42301 caacagattt ctgaaatgct atcttaccgt tttttccaaa tgctttttct attgcagaac
    42361 cattattttg ttcagcccat ataaaatatc ctccattttt agctgatact tttaagtaat
    42421 ctgccgcctt attttcaata tcatttgccc aaatccaata attctcagta ctgaaaatac
    42481 catgcaaatt tggatatttt tgatacattt tatcaatcca gctggtactt aaccaatgtg
    42541 ccgaagtata ataaggcata ttaccagcag tgtaaaccgt tagaataact ggaataggtt
    42601 cattttttcc agtcttagga tttacatatt tcgctgcttc ttctagcata tgttcataaa
    42661 aatctctagc agctccatct cttttaggaa gatcatctgg atgtaactgt ataacagtat
    42721 atggttttaa gtcatctggt atattctccc acttacctgt taatgtatct cctccccaaa
    42781 aagtatattt cccttggtta aactcttctc cgttagcata taaaggcatc ataagtaacg
    42841 gatgttcatt attaatttca cgacgtttat caacttctga ccaatcatta ggattcttaa
    42901 atttaccatt tgccataaac aaggctccac ctaaaaccaa ttcgtcagcc catgtatatt
    42961 tccctgaata tgatttaagt tctattgtct gtgtatcttt gggaatttca acatcaacca
    43021 gaaatgccga tgtatcatac ttaattcctt ttggatattt tgaatctgat gagtaaatga
    43081 ctttaccatc cgcaacaact tcaaattttt gaatatctgc gtaatctgaa tttaaatgat
    43141 ttgcactcct atcaattccg acataggaaa taaatcttgt aacattagca cccgaaacat
    43201 catattttat actagacggg ttacttgcta cagttcccaa tcccttatta aattcgactt
    43261 ccttaccgtc aattagtaat tttattttat tattagaccc gttattacca ggtgtaaacg
    43321 gtttatcttt ctgaaccgtt tttgatttat cgtcatcacc gtgagtagca tcaacccatt
    43381 ctaaatcaga aagatatact cctccattaa ttcctttatt aagaagatct ggagttatag
    43441 tttgagtact aacagtttta ataagtttag catcagcaaa tacaacctca tcaccccatg
    43501 tatgttctcc tgcgaatgat tttaaggata tttttttagc gttttgtgga attgtcacac
    43561 taataaattg tgcctgggta ttatatctga atccttctgg attagttaca ctactagaat
    43621 agacaacctt tccatcaatt tctatttcta ttctatccac tactgcatgg tctgatctag
    43681 aactattagc actttgatca attcctatat aagtttcaaa tttttcaaac ccttgaccgc
    43741 tgatatcata gctgtttact gatggagaat ctgcgactgt tcctattcct ttatcaaaat
    43801 atttaacttt atcatcagaa gtgagcaatg aaatcttagt atgctctcct ttatttcctg
    43861 ttgtaaatgg agcatctttt tgtactgttt ttgttttatc tatatcacca tgagttgcag
    43921 aagaccaatc catatctgaa agatatgtca tctctttatc aatttcttta tctttagtaa
    43981 taagtttttc taagttattc cctgttactc tagcagtttc agtactagct ctgttattag
    44041 aattactttg aactacatct gcataaacca aattacttcc taaaaaactt gttaaaacag
    44101 ccgtagctat taaaatctgt tttgctattt ttttactttc catttttatt tcctcaatat
    44161 ttacatacaa cttcttcaat aacaaataat tttgtaagat attctggaac acaaagtgtt
    44221 aaattactta aacttacttt tattccagca ttcattaatt catctccata aaagaactca
    44281 tcaaatctag agacggaata gcatttatct ttatctaaac cactaaaata aactcttgta
    44341 taacctggat tcaacttact attcaattga gcatacaata aaatcgattg gcatttatct
    44401 tttgaaacaa cttgccaact agcagaatca caactaaaag gactgtttat cctatagaaa
    44461 tccccatatt gaaacaatga acgatatttt ttataaaact gaatttgttc tctcacttgt
    44521 tctttttctt ctaccgacaa tctcgtaaca tctaattcat aaccgaaaga actaaaataa
    44581 gctacatttg tcctagtgtc caaactcgtt tgtcttccac tttgttcatt tggagaaata
    44641 gaaacatggg ctgtcatcat tgatggagaa tatccataag aagttccatg ctgaattttc
    44701 aatctttcta tcgggtccgt atcatcactc gtccacgctt gcggtgcata atacataagt
    44761 cctaaatcaa aacgtccacc acctgaagaa caagattcaa atagaactga agggaactta
    44821 gttattaaac gatctaataa ctgataaaga cctaagatat atcgatgacc aaattccatc
    44881 tgctgttcag aatcaagttc aatactataa atatctgtta tatgacgatt catatcccat
    44941 ttgatataat ccagatttgt tttgattatg attttagaaa tagaatcaaa aaggtaatca
    45001 acaacttcct gtctcgtcaa atccaacaca tattgatttc ttcctactga cttctctctg
    45061 tctaacaaat ggatagtcca atcggcatga ttcttgtaca aatcactatc aatagaaatc
    45121 atttcaggct caaaccacaa accaaattgt aaacctattt tgtgaatttc atctgcaaga
    45181 aatccaatac cttcaggaag gcgacttcta tctgttaccc aatcacccag agaacttttg
    45241 tcatctttcc tatgaccaaa ccaaccatca tccagtacaa ataattctat ccctaaatct
    45301 ttagcagcct ttgctaaatc taaaattttt tctgtactca agtcaaaata aaatgtttcc
    45361 caactattga gtattatagg acgactagct tgagaaaaat taggattaac taaatgatgt
    45421 cgataaaaat ctgatagttg ttggctcatc tgattcatcc cttgatcaga atagatcaca
    45481 attgcttctg gtgtcacaaa actttcggca ggatttaata aaaaacgaaa ttctactgga
    45541 tttattccta ccgttactct agcggtgtca aaatggtcaa cttcaatcat attttgaaaa
    45601 tttccagaat agataaagtt aaaaccataa acctcaccac tatgctctgt agtttccctc
    45661 cttgatagca taaagaaagg attttgctga ggacttgatg agtatctcaa gctatctatt
    45721 gcttgaatac ctggtctaag cgaagttcta tataactgac gttccctcag ccatgctcca
    45781 gaaaattgaa taaagtcata ttgactatca ggtagatcta gctgtaagct cattgctttc
    45841 aaaagagtca acttattatt gctattattt aaaaattccg tatttctaac taagacattg
    45901 tattccggaa aaattgtgta atttagtcta acagttaaat ctaatatgga atctgttaaa
    45961 ataatcgtta atgtttccgc acactgacca atatcatcaa atgttgaagg tagagagtta
    46021 agacgatttt tccctttttc aagttcaaaa ccttgatatt ttagtgtcgt tatcctacta
    46081 ccattcattc cttgaacttg aaaggctgga tgccgaaaat ctcctttccc atatgaagaa
    46141 tattcatttt gtatcataga ctgttgaata tcctcaaatt cttcagaaaa agatacttca
    46201 aatcctcttt tttcataggc catcaattgg ttataatttt ccttgacatg aatccttttc
    46261 ccaaaatgta gatgttgaag ttccccattt tctaaaattc taaaaatata actactatct
    46321 ttcattgcca aatgaaaaac ggtctcgtcc ttattaatat aaatcgtcat ctctacctca
    46381 caattttttc tttacttctt cagtatcacg atagagtacg gtgccaattc tatctcatct
    46441 tcttgaatag taaaatctcc aattactctg gtaaaattct ctatcgaaag attctctatt
    46501 tttatttttt gtttaatctg agcaggattt ctcagagaga gaatttcagt agaagtcttc
    46561 tgattgtgac acttataacc ataaatttca aatttttcag gacttcctcc tattttttta
    46621 ctatatttta gtgtttgata attctcctct atccatttga cggcacgagc attggctttc
    46681 cagcgttctt catcaaacat atcaaaagaa tagtgaaact cccaaaaagc attccctctt
    46741 gttgaaataa ataatagata agcttcaaaa tctggtattg atgcaaacat ttgatgatcc
    46801 atgtaccagg tatttgcact gacagcatag attggttcat gattataaag cgaccacata
    46861 ggtaactgta tctcacgttt ttccaaaaat tcttgatact gaatatctcg gtaagttatc
    46921 atacgattga tatcattacc tgcattctct gtaaagccta catcttgaga tatttgaatc
    46981 cataaactat tgacccactg taaaaaccat ggactaggat ttacataaga agtcaagttt
    47041 aaccaacaat cttttcctcc tctctccttt cttagatcta tcaacagttg aattaagaac
    47101 tcataaacca ctgtcatggt atacatacca tgcggtccac tcttatcagg tttgtcaggt
    47161 tgaagtaacc agccatcaat tttccaatag ctgatatcga attctttttg atattccaac
    47221 atccttttct tcatttgatt gagatagtta aaatcagcca cgtttacatc atttgaaatc
    47281 aaatttttag atccaatatt taactctgga tgtgcttcta accaatcact cataatgatt
    47341 tctgtcccat tataaccacc tcggggacca atccacaatc ctaggcttga accaaatcca
    47401 tttacaagat gtttaatatt tctcaaacca tttgggaatt tatgattgaa ttcccaaact
    47461 gattgatagt ttgtccaacc atcatcaaca acataagcat ctaaatgaac tccatgattt
    47521 tcaaatccat ctcgaatctc agaaaaactt tttaaaatac cttcctctgt aatatcggtc
    47581 atatggtcat accaggaatt atactgttta cggaaataac taggttgagc tattccttca
    47641 acatattcaa aaaatgcctc ttgaatctct tcttttttat aagaacatgc tcccccaata
    47701 atacaagacc acaaactctt ttcttgattt acaacagttc ccaagtaata tcttgataca
    47761 tagtgtctac catctacctt attttcagac attggaaatt ccattcctaa gaataaagaa
    47821 ttcgcataaa caggttgtcc caattctaca taataaccgg aaaaattcgc catttcctta
    47881 atacaatctt gtcttttcgg atagaaaata ttgtcttctg tttcaaactc aaaactctct
    47941 acctctacag atttaataat ctcagtagat gattctaaaa ctaattgctt cgaaagaaca
    48001 tcatccctcg attcaaagat taaacgtaaa cgaatggaag gacaaacaaa agaaacctga
    48061 attcgcgtat ctccctcttc cctaacgatt gctgaaaaat gatttgaaga aagtcttata
    48121 ccattctcca atgtaatggt aaattcttct ttcgaaacat tttttatcat tactcctgcc
    48181 cgtttgttta cgatatcagt agtataaaat cgaccatctc cccaaaagaa attacgtctt
    48241 acatttttat tttcaatttt catataaact actctctcaa ctcaattttg attacgctat
    48301 caatcaagtc tggtaatgga taggtaaaat gtggaacttc tccaaactgt gcaaaacaaa
    48361 ttcctttgta ggcattggtc gtccagcttt ctgaaatttt cacctcactt ccatcatgaa
    48421 gaaagctcat tctttttacg ttttctttac taataccaag aagagctaaa ggacctatag
    48481 gttgttcaaa tacatgagca taaaccgtat ttccattacg agtatagtat ccccaatctg
    48541 gttttggtaa attttcatct ccactacatc cataaattgc ctcaccattt cgagacatcc
    48601 atcgatgaaa attatctaat attttcttac ttgaatcatt aattcttccc aaagcatctg
    48661 ggcctacatt aagaatcata tttccgtttt tactaacaca ttccactaac tttcttatca
    48721 aagtttgact agacttatat agataatctg taggattata agcccaattg ttattcatag
    48781 ttagacaaag ttcccaagga acaggttcac ctttaaagtt tcgaatacct tcgtgaggaa
    48841 caatttgctc tggactaaca aaatctccag cgtaagaggt aatttcatct gtaacgatac
    48901 ttccgaatcc ttcacctgat gtttctaatc tattatctac gataacgttt ggctgataat
    48961 gacgaaccat gtctattaat tttgaagctc cccatttttc acccaccatg tcttcataag
    49021 aataatcaaa ccataatatg tcaatctgac cataaccagt tacaatttct ttaacttgat
    49081 tgtgcaaata ctccaaataa ctgtcaaagt taattttctc atctcgatag gcttcatttc
    49141 ctctcattgg atgatttaga tcagcatatt tagggaaatc aggatggtgc cagtcaatca
    49201 aactaaaata taagcctact tttaaacctt cagctcgaac agcatctaca aattctttaa
    49261 ctaaatctct acctgcaggt gtatttgttg ctttataatc tgtaaatttt gaatcaaaca
    49321 aacaaaaccc atcatgatgt ttagccgtta aaaccatata cttcatccct gcagcttttg
    49381 cttgtttagc ccattctcta gggttatatt ccttaggatc aaaagctcga aaatatggtt
    49441 cataatcttc aatagataat ttctgatgac tacgaatcca ttcccctttt cctggaatag
    49501 aatataaccc ccaatgaatg aacatcccaa atcttgcttc tctaaaccac tgagttctac
    49561 tattgatagt ttcaagtgta tgtttcatcc ttttactgct cctcctccta aagcttcaat
    49621 gaagtattta cttgtaaatg cgaatagaat gattggtggt aatacgatta caacaatagc
    49681 tgaaaataga ccattataat tagtattaaa acttgtagta aattgaccta gtaatgctgg
    49741 tactgttaat tcatttttat caatcaccaa catagatgcc caataatatt cattccagtt
    49801 attaataaag gcaagtactg ctgctgttaa aattcctggt ctagcaattg gcaacatcac
    49861 tttccaaaaa gtttgagcat atgtcgcacc atcaatctct gctgcctcct ccaattcttt
    49921 tggaataatt tcaaaatagt tctttataat aaagaaactc atagcaattc cactacaaga
    49981 gtagaccaga attagagcaa ctggggtatt ataaatcccc aactcattta ctaatctata
    50041 aactggaaaa gtcatagctg ttgctggaat aaatagagta gaatacagca tagaagtcac
    50101 caacttcttc cctctaaatt ccatcctagc aataacatat gaagacatgg agatcataac
    50161 aatgctaata ataactgata tagacactac tttaaagcta ttccaaaagt aagtaaaaac
    50221 atgaagtttt gtaaaaacat ttatataacc ttcaagagta aagtcagtcg gtaaactaat
    50281 acctggattt ttcataggat ctgcttttaa tgatgataaa aaaatccacg caatagggaa
    50341 aatagaaatt aaaatggtaa ggagcatata accatatata agaactttgc cttttttact
    50401 aataccattt atatgtgtat tcataatcat ttcccacctt tcagtctttt tctttctctg
    50461 aataaaatat taatacaaac gataatcaac attccaccta agatttgtat aactccaatt
    50521 gtatttgcac gagcatactg agttcgtgag ctagccactg ctaattctcg aataacaaaa
    50581 ctgatacttt ttgttcctgc agcaccattt gtcaaaaaga atacttcgtt atagagcaaa
    50641 aatcctgaag ttgctgccat aattgaaaca gttttaattg tccccttaat catcggaatc
    50701 gtaatatacc actctttcct ccaaccagta atattgtcta aaatagcagc ttcttgaact
    50761 tcttctggaa tagcaaaaat ttgcccaaga atcataatgg ttgatgctcc tgcaaagaag
    50821 atataagcgc cagtcatagc tactatattt aatcctggag tcaacagtac tgaatcttta
    50881 aacgatggat taaatagatg aataatttgg ttaacaacac catagtttgg attatagatt
    50941 tgaagaaaaa tcaagcccat agcagcacta gaaatgatac ttggtacaat ataaagattc
    51001 ctagatatct tccagccttt taatttctta gataatgtaa ttgcaacaga tacagctaaa
    51061 ggaacttgaa taataacacc aattacagcc caacgaacag aattaattag agcttctgta
    51121 aaaaatgggt attctttaaa aacataccta taattttcaa ataatttatc aaatccaaga
    51181 aactcagggg tgtttaaatt tgtataatcc catttagcaa atgaagttaa aaagattgtt
    51241 actacagaat aaaaataaaa tagaaagaaa caaatcaacg ttggcaaaag aaataaaaag
    51301 ataaatcgtc ttttttgatt cttcctattt tgtaggctca taattttttg tgccataaac
    51361 aaactccttt tttcaactat ataagtaaaa tcaagctcag ttggcagtgc caactgagct
    51421 tcgttttcac tccctaaaac aattaaccaa tcaatgattt caacacatct tgagtagatt
    51481 ttactttttg atcaacatct acattttcag cagcactttc tgtcaatgca ttgataatag
    51541 ctgtctgtac atcgccaccc caaacatcac ttacagttgg aacaactgct tttgcattct
    51601 taacttgagt gattgcttgt cctaaaattt tagtagtagc ttctgaactc ttttctgaaa
    51661 gttcttttac atttacattt tcattagaag gatttgctcc tactttttca aaaattactt
    51721 tttgtacatc atcgctagtc atatatttca aaaactcaag agctagtttt tgtttagctt
    51781 cagacatctt acttgagata gtgataccac ctccagaaga actaattgct acacctgcag
    51841 gatagattcc aggtgcaaga cttgggttct tagacatctc accagaagcc cacacaccat
    51901 taaagaatac tgcagattta ccttcttgga aatctttgga ataagcattg gcatcgccac
    51961 cagcattctt agaaccatta gcttgaattt ctttcataac cattttcaaa gcatctgcaa
    52021 attctttaga ttcaattcct tcttttgtta atggtttatc taataattta cgtccatttt
    52081 ctgtggttcc taatactgta ttaaacaagc gaatagatgg ttctccagca ccaaatgcat
    52141 acactccatc ttgttttcta atgcttgcca ttgcctgagt gaaatcatcc caagtattcc
    52201 actgatcagg tgtcttagca cccgcttttg caaatatgtc cttgttgtac cacaaaccca
    52261 ttgtaaataa ctgttcgtga actgtataaa tttttccatc tttttggttt tgcttatagt
    52321 tcaaaccaac atttttttca agcttatttg aatcgatata tggtttcaaa tctaaaacaa
    52381 gtttttgttc aatcgcagct aatgatactg cattacctgc taggtcaatc acatcaggaa
    52441 attcaccact agcaaccttg ttattcatta tatccttcaa atcaccattt acaggagttg
    52501 gtttaaattc tacaccacta tctttatgtt cttctgcaaa tttatcatat aaatctcgca
    52561 tagtcttcgc tacaggccat tcatcttcac tatgatgata cccatgataa aattctaata
    52621 cttctttacc agaagaactt ccaccgcttg catccttaga tgttccacaa gctgctaaac
    52681 caaaggtagc tactaacccg ataacagcgc atttcaaaag actttttttg ttcatgacaa
    52741 aacctccgtt tttttatttt tgtttatgat agctgaggat aataatattg tgtctcagca
    52801 atatttgtca taatatcctt taactctgta tcctcgttta ttttaccttg actcaatagc
    52861 tgcacaacaa tattccccac agctgtaccc tcagtcaaac ctgtaagaac ctctttacca
    52921 gttctatcag caatcatttg gttaaagtaa ctggctctag cacctcctcc aatcacatat
    52981 atcctcttat aaaccttatg agttagttct tctagtaact ctatcgcttt cctatacgtt
    53041 tcagctaggc tttcataaac aatcttaaat agttgtccat ctgtccactc tctagtttca
    53101 tgatgataag ctagatattc tgtcaaagtc ttgtgcatat cagattctgt tgcaaattca
    53161 gttgattcag tatcaatcag aggaagattt tctttttctt tctccaccat tgtcctaata
    53221 tcatcaaaag aataggcttt ccctcttcgt tcaaatgaac gtcttagttc ctctatgatc
    53281 cacaaccctg tacaattctt cagaaatgta atcactccat ctttaccgac ttcatttgta
    53341 aatccataac tgaaggattc ggtagtaaga agcggtgaag taagttccac tccaaccaaa
    53401 gaccaagtac ctgatgaaat aaataaacta ccttctgtct taggtactga gacaatcgcg
    53461 cttgctgtat catgactaca aacattcaca acaggaatat cgcctaaacc atactccttt
    53521 tttatccttc caagaacatt tccctctgaa acaatctcag gaagtaaaga tgaatccaat
    53581 tcaaatagtt ttaagatatt ctgattccag ttttgactcc taggatcaaa taattgagtt
    53641 gttgaagcaa tgcttttttc tgtagcaaac ttacctgtca agagataatt aaacaaatct
    53701 ggcattaaaa gaatcttatt ggtcttatag aaagagtcag gagattcttg acgtgcctta
    53761 aagagttgaa acaaggtatt tatctccata atctgatttc ctgtctctga atacagtttt
    53821 tctaattcag tcatttcaga tatttccttt aacactccct ttgttctttc atcacgataa
    53881 tgaacaggtt gtaataacag cttaccttca ttatcaacca gtccaaaatc aactccccat
    53941 gtgtcaatac cgatagataa aatcttgtaa ctagtattag ccaagcggat actttcaaga
    54001 attttagcta gtagaaagtc aatatcccaa gataaatgcc cttttactct aataggtaga
    54061 ttagaaaagc gatttatttc ttccattact agtttatttt cagaaaggta accaacgatt
    54121 gctcttccag aagtcgcacc taaatctatt gctaaaaccg cttccatttt tcttctctct
    54181 tcgtccttga ttaactttat tataatcccc acgttttatt ttttcaatat gtgaacgctt
    54241 acttgaataa tgttcaaatg ttaacccaat attcataaaa ataattcaaa ttcctccaat
    54301 ttgtgctata atatagttga aatgataaat taggaggagc ttatgataaa agatgaacgt
    54361 gtacttgaat tgattgaaat catcaaaaag aaaaaaagaa ttgccgtaaa agagctggca
    54421 gaaatcactt tctccagcac aagtacctta cgtcgtgatt taattttctt agaaaatcaa
    54481 ggtcttatca aaagaaagca cggatacgtg accctgtcct ctatgaacac aattgaactt
    54541 tctcatcaaa tacgggaagg agaaagtact aggcaaaaaa gactaatcgc tagtctcgct
    54601 aaagacttta ttcggtcagg catgtgtatt tatctggatt ccagtacgac tgtctacgaa
    54661 ctttgtccct atctttctga acttgataat ttgattattt ttacaaatgg tttacatact
    54721 gcacaaaccc tatctgaaac tgttaaagat agctccaaaa tctttatcac atctggcgag
    54781 gtcaaacatc aatcctgttc cgtggtcaac tatgataagg aaaattcttt attagatcat
    54841 tttaatatcg atttagcatt ttgttcagca agaggtattg atgaccaata tgtttatgaa
    54901 gcttctctca gccaagctat ttcaaaaaag aatattattg acaaagccca tgaaaccatc
    54961 ttactgattg atagttctaa attttacaag actggatttt ttaaaattaa tcccctctcc
    55021 aaatacacaa cctttatttc tgacaccttg ccaaatcaaa aattattaga cgcagtcgaa
    55081 ttatttgatg gagaatgggt ttctgatatt caatgaaaat caaataggaa gctagtctca
    55141 gcttgctcaa agtacagatt tgaaaattga tcaagtcagc tcgaaacact gttttgaggt
    55201 tgtagataga actgatgaag tcagtcacat atatacggaa agacgaagct gacatgatgt
    55261 gaagagattt tcgaagagta tcgttctgtc tgctgactga tttacaaata ttcaccacat
    55321 gacattattt tcttagcaac cctaaaaaca gaggctggac aaaaactcaa tttcaggaga
    55381 aaatggagta aaccttccca caataaaacg catagtatca agtttttgca cacctgatat
    55441 tatgcgtttt tctgatttta aagatttttc tcttagatct gatatttcaa gaattaagtg
    55501 accttgatga tctaaatgta ttgtggtata ctatttttgt catcggttac cgattacgtt
    55561 cctatggggt cgtgagagga ggtgaaacta atgagcctta tcatagagct cgctcttact
    55621 attatagcgg atgttatagc tggaattatc ttgtatttcg tctgcagatg gctaaatagt
    55681 aaggagtaga ccgagtgact agcctataaa cacccgttaa atcgctaaga tacgtcaaaa
    55741 aagcccttaa ctacggccat agttaggggc tttggtgttc taatgaacct tatcaattaa
    55801 ctacatttta gcatataatc tacaatagtt caagcattaa gtgaggttga tgatctaaat
    55861 ttagtgtggt atactatttt tgtcatcggt taccgattac gttcctatgg ggtcgtgaga
    55921 ggaggtgaaa ctaatgagcc ttataccaga gcttgctctt actattatag cagatgttat
    55981 agctggaatt atcttgtatt tcgtctgcaa atggctagat ggtaagaagt agaccgactg
    56041 actagcctat aaacacccat taaatcgcta agatacgtca aaaaagccct taactatggc
    56101 actagttagg ggctttggtg ttctaatgaa ccttatacac taactacatt ctagcatata
    56161 agcccagata tttcaagagt tttatttatt ttttcaggtt cccttagttc tgaaaggtc
//
LOCUS       DAVDWM010000008        55641 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVDWM010000008 DAVDWM010000000
VERSION     DAVDWM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.008"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..411
                     /locus_tag="VUW49_000605"
     CDS             <1..411
                     /locus_tag="VUW49_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7980563.1"
                     /translation="IQDILSVAPRSIGTTSPAREFEIIKHYKRLIDKAETCVNDLMAE
                     SNSVITTVTGIGNRLGAVILAEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMI
                     KRGSPHLRWALIQAAKACARFSPAFKAYLKTKLE"
     gene            complement(712..2601)
                     /locus_tag="VUW49_000606"
     CDS             complement(712..2601)
                     /locus_tag="VUW49_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET7980564.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2739..4304
                     /locus_tag="VUW49_000607"
     CDS             2739..4304
                     /locus_tag="VUW49_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET7980565.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4304..5044
                     /locus_tag="VUW49_000608"
     CDS             4304..5044
                     /locus_tag="VUW49_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7980566.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(5153..5368)
                     /locus_tag="VUW49_000609"
     CDS             complement(5153..5368)
                     /locus_tag="VUW49_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980567.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(6239..7489)
                     /gene="ilvA"
                     /locus_tag="VUW49_000610"
     CDS             complement(6239..7489)
                     /gene="ilvA"
                     /locus_tag="VUW49_000610"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET7980568.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7539..7895)
                     /locus_tag="VUW49_000611"
     CDS             complement(7539..7895)
                     /locus_tag="VUW49_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7980569.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(8133..8369)
                     /locus_tag="VUW49_000612"
     CDS             complement(8133..8369)
                     /locus_tag="VUW49_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980570.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(8476..9498)
                     /gene="ilvC"
                     /locus_tag="VUW49_000613"
     CDS             complement(8476..9498)
                     /gene="ilvC"
                     /locus_tag="VUW49_000613"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7980571.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPSLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9564..10040)
                     /gene="ilvN"
                     /locus_tag="VUW49_000614"
     CDS             complement(9564..10040)
                     /gene="ilvN"
                     /locus_tag="VUW49_000614"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7980572.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(10033..11733)
                     /locus_tag="VUW49_000615"
     CDS             complement(10033..11733)
                     /locus_tag="VUW49_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET7980573.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11934..13601)
                     /locus_tag="VUW49_000616"
     CDS             complement(11934..13601)
                     /locus_tag="VUW49_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET7980574.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQESMTAALRD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13604..13969)
                     /locus_tag="VUW49_000617"
     CDS             complement(13604..13969)
                     /locus_tag="VUW49_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7980575.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(14126..14314)
                     /locus_tag="VUW49_000618"
     CDS             complement(14126..14314)
                     /locus_tag="VUW49_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7980576.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(14430..14606)
                     /locus_tag="VUW49_000619"
     CDS             complement(14430..14606)
                     /locus_tag="VUW49_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET7980577.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENITQDLI"
     gene            complement(14717..14914)
                     /locus_tag="VUW49_000620"
     CDS             complement(14717..14914)
                     /locus_tag="VUW49_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET7980578.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(15342..16886)
                     /locus_tag="VUW49_000621"
     CDS             complement(15342..16886)
                     /locus_tag="VUW49_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET7980579.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNELSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            17099..17401
                     /gene="gatC"
                     /locus_tag="VUW49_000622"
     CDS             17099..17401
                     /gene="gatC"
                     /locus_tag="VUW49_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7980580.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            17401..18867
                     /gene="gatA"
                     /locus_tag="VUW49_000623"
     CDS             17401..18867
                     /gene="gatA"
                     /locus_tag="VUW49_000623"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7980581.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TIQSAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSIAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18867..20309
                     /gene="gatB"
                     /locus_tag="VUW49_000624"
     CDS             18867..20309
                     /gene="gatB"
                     /locus_tag="VUW49_000624"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET7980582.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISNEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            20438..20998
                     /gene="efp"
                     /locus_tag="VUW49_000625"
     CDS             20438..20998
                     /gene="efp"
                     /locus_tag="VUW49_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7980583.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            21020..21409
                     /locus_tag="VUW49_000626"
     CDS             21020..21409
                     /locus_tag="VUW49_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7980584.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            21402..21824
                     /gene="nusB"
                     /locus_tag="VUW49_000627"
     CDS             21402..21824
                     /gene="nusB"
                     /locus_tag="VUW49_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7980585.1"
                     /translation="MTSPLLESRRQLRKCAFQALISLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21874..22110)
                     /locus_tag="VUW49_000628"
     CDS             complement(21874..22110)
                     /locus_tag="VUW49_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980586.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(22123..22574)
                     /locus_tag="VUW49_000629"
                     /pseudo
     CDS             complement(22123..22574)
                     /locus_tag="VUW49_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22592..22777)
                     /gene="briC"
                     /locus_tag="VUW49_000630"
     CDS             complement(22592..22777)
                     /gene="briC"
                     /locus_tag="VUW49_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET7980587.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22935..23702)
                     /locus_tag="VUW49_000631"
     CDS             complement(22935..23702)
                     /locus_tag="VUW49_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET7980588.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQVVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(23699..24565)
                     /locus_tag="VUW49_000632"
     CDS             complement(23699..24565)
                     /locus_tag="VUW49_000632"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET7980589.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(24602..25969)
                     /gene="accC"
                     /locus_tag="VUW49_000633"
     CDS             complement(24602..25969)
                     /gene="accC"
                     /locus_tag="VUW49_000633"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7980590.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVKKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25981..26403)
                     /gene="fabZ"
                     /locus_tag="VUW49_000634"
     CDS             complement(25981..26403)
                     /gene="fabZ"
                     /locus_tag="VUW49_000634"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET7980591.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(26400..26885)
                     /locus_tag="VUW49_000635"
     CDS             complement(26400..26885)
                     /locus_tag="VUW49_000635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET7980592.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26888..28123)
                     /gene="fabF"
                     /locus_tag="VUW49_000636"
     CDS             complement(26888..28123)
                     /gene="fabF"
                     /locus_tag="VUW49_000636"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7980593.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFIKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(28145..28876)
                     /gene="fabG"
                     /locus_tag="VUW49_000637"
     CDS             complement(28145..28876)
                     /gene="fabG"
                     /locus_tag="VUW49_000637"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET7980594.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQVGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28910..29830)
                     /gene="fabD"
                     /locus_tag="VUW49_000638"
     CDS             complement(28910..29830)
                     /gene="fabD"
                     /locus_tag="VUW49_000638"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7980595.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(29823..30797)
                     /gene="fabK"
                     /locus_tag="VUW49_000639"
     CDS             complement(29823..30797)
                     /gene="fabK"
                     /locus_tag="VUW49_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET7980596.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(30916..31140)
                     /locus_tag="VUW49_000640"
     CDS             complement(30916..31140)
                     /locus_tag="VUW49_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7980597.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(31200..32174)
                     /locus_tag="VUW49_000641"
     CDS             complement(31200..32174)
                     /locus_tag="VUW49_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET7980598.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(32174..32608)
                     /locus_tag="VUW49_000642"
     CDS             complement(32174..32608)
                     /locus_tag="VUW49_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7980599.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(32702..33487)
                     /locus_tag="VUW49_000643"
     CDS             complement(32702..33487)
                     /locus_tag="VUW49_000643"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7980600.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33812..35176
                     /locus_tag="VUW49_000644"
     CDS             33812..35176
                     /locus_tag="VUW49_000644"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7980601.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            35179..35550
                     /locus_tag="VUW49_000645"
     CDS             35179..35550
                     /locus_tag="VUW49_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET7980602.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            35764..37038
                     /gene="serS"
                     /locus_tag="VUW49_000646"
     CDS             35764..37038
                     /gene="serS"
                     /locus_tag="VUW49_000646"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7980603.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEIKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(37063..37962)
                     /locus_tag="VUW49_000647"
     CDS             complement(37063..37962)
                     /locus_tag="VUW49_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET7980604.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(38072..38620)
                     /locus_tag="VUW49_000648"
     CDS             complement(38072..38620)
                     /locus_tag="VUW49_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7980605.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38838..40160)
                     /locus_tag="VUW49_000649"
     CDS             complement(38838..40160)
                     /locus_tag="VUW49_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET7980606.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGVYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(40251..40339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(40502..42838)
                     /locus_tag="VUW49_000650"
     CDS             complement(40502..42838)
                     /locus_tag="VUW49_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET7980607.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQLLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42942..43490)
                     /locus_tag="VUW49_000651"
     CDS             complement(42942..43490)
                     /locus_tag="VUW49_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7980608.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPVTTSWLKQIWVTNLIG"
     gene            complement(43487..43789)
                     /gene="zapA"
                     /locus_tag="VUW49_000652"
     CDS             complement(43487..43789)
                     /gene="zapA"
                     /locus_tag="VUW49_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7980609.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43876..44757
                     /locus_tag="VUW49_000653"
     CDS             43876..44757
                     /locus_tag="VUW49_000653"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET7980610.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQITPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            44761..45375
                     /gene="lepB"
                     /locus_tag="VUW49_000654"
     CDS             44761..45375
                     /gene="lepB"
                     /locus_tag="VUW49_000654"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7980611.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            45455..45577
                     /locus_tag="VUW49_000655"
     CDS             45455..45577
                     /locus_tag="VUW49_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HET7980612.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADES"
     gene            45654..48020
                     /locus_tag="VUW49_000656"
     CDS             45654..48020
                     /locus_tag="VUW49_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET7980613.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMILAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDRVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(48068..49351)
                     /locus_tag="VUW49_000657"
     CDS             complement(48068..49351)
                     /locus_tag="VUW49_000657"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7980614.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYSKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(50137..51273)
                     /locus_tag="VUW49_000658"
     CDS             complement(50137..51273)
                     /locus_tag="VUW49_000658"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET7980615.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIEQLENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYVFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(51335..51772)
                     /locus_tag="VUW49_000659"
     CDS             complement(51335..51772)
                     /locus_tag="VUW49_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7980616.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(51774..53729)
                     /locus_tag="VUW49_000660"
     CDS             complement(51774..53729)
                     /locus_tag="VUW49_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET7980617.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTGLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESI
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRAYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(53753..55522)
                     /locus_tag="VUW49_000661"
     CDS             complement(53753..55522)
                     /locus_tag="VUW49_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HET7980618.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIIATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASSRYDEIVASLTGASPIAEIEGDIPTSAPVDSQESDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT        16479 a        12610 c        10368 g        16184 t
ORIGIN      
        1 attcaggata tcctctcagt cgcacctcgt tctatcggaa caacatctcc tgctcgtgaa
       61 ttcgaaatca tcaaacatta caagaggctc attgacaagg cggaaacatg tgtcaatgac
      121 ttgatggctg agtccaactc ggtcatcacg acggtcactg ggattgggaa tcgtttaggg
      181 gcggtcattt tagccgagat tcgaaatatt catgcctttg ataatcctgc tcaattacaa
      241 gctttcgctg gactggattc ttctatttat cagtcaggtc agattgattt agctggaaga
      301 atgatcaaac ggggttcccc tcatctgcgg tgggcactca tacaagctgc caaagcatgc
      361 gctcgctttt cacctgcttt taaggcctat cttaagacta agttagaata aggaaaacat
      421 tacaatgtag ccatcatcca ccttgcaaaa aaacttatcc gaaccctgtt ttatatccta
      481 aaaaagagct gccatttgac gaacaaaaag tgatgtaatt tctgaaatta acacaggatt
      541 acctttgaaa aaatggactt tttcaagggc tttttgccgt gctctaaaat catcgccaaa
      601 tcatcaaatt gaaataagaa atagtttaga taaaactatt gacttttctt atagtttgtc
      661 tttcttctat cttccattat atcatacttt ttcagtctat ggtagctctc tttaaaaggc
      721 tccgatactg ccgccacctc cgcctccaga gaagccaccg ccagaacttc cacttccaga
      781 agatacggag taggtacttg ctgtatttgc gacactagca taatggctca tttgcgcgct
      841 tgaatgataa aacatactgt gccagccata agctacatag agattgatat ctggattttc
      901 aacttgaatc tgatgaacct tcatcaaatg actaaccttg tccgcatagc caaataaggt
      961 tgcatagacc aagagacgat tccaaacaac aatactttcc aattcagcct tatccagtcg
     1021 tgcaatctca cgtaacatat tttcaaaact ggtccagaga tagtagacct cagctcccgc
     1081 ttcatttaga acaccatcac gattatctag tcgaagcttc caataataga aaacagacaa
     1141 aactaaccct agaaaaccaa gtattggcaa agggaggtaa agatagccat gaacgtctaa
     1201 actgtacaag aacaaaccaa atccgataaa taggggcaag atagtcaaga cacccatacc
     1261 cacttgcaaa gtcttttcca aaccagttaa aggacgatag taatctggga gcccccagaa
     1321 ggaaactcgt tttctcactc cttcttgcat ctggttcaat acctcttcaa aagaagattt
     1381 gagttgaagc cctcttgctt gaatccgttt ttcatcagaa actttggctc tacgataaag
     1441 actatcagat accttgtaat ccgcaaacaa attggaaaga gtggcttctt ttttgcctga
     1501 aaaggctaga tttagacagt ctctctcaaa tcttgacaaa ccatcttctt tcaccaatct
     1561 caagccaact gcatctcctt ctgaaataat agagacattc ccacggtcta tcacatctag
     1621 caaggtagct tgaataagtt gatcaaaggt gaattttcca gctcccttga ccaagggact
     1681 cacttcctcc aaggaggtcg agtagactgc ttctgataaa accataggct ctaattccat
     1741 tggtggttca tagagacgat gatttttggc atatttgact gaaggagtgg tctttcttct
     1801 ataaataaaa tagaagcaga cactcaataa caaggagata gaaaggatcg aagggaggac
     1861 ccaagtaacg agttgtttac tctgatcttt ttctctaaca atcgagtctt ctatcttatt
     1921 aaactcttct aaacgattcc ctttcaagcc ctgatcccta gcgctagcaa aatcggtccg
     1981 agaccaatag gcatgcaact caactccacg cttagccgga agattgtcta aacggatagt
     2041 ataatcaagg ttactctttt caatcgttcc ctctctaaaa agtttccctg tatggaaaaa
     2101 gagtttttca gcccccttgt ctccccttac atgaaattca aacttttcaa tagactctga
     2161 actatctgtc agaggttgcc aatttaattc agcgatatca tcataaagga aaagtaaatt
     2221 ttttaagttc cagacgaggt caacttcaac tatgtcgccc tcctgacctg gattatagac
     2281 tctcacagta taaccatccg cttcttctgt tacttcgcta gtcacatctg ctagttctgc
     2341 accgtttttc gcagcctgaa cctttggatg agggtcaatg tcaaacccgc taggcatctt
     2401 accagcacgt ccaagtccca cgatttggcc cttaaagtcc tcctcaaact ggtaaactat
     2461 cttctgtcta aactctgccg tattatctgc atgaatatac aaatcccctt gataagagtt
     2521 tatcttgaaa tcaatggcaa aaacagagag tggaagaagg caaaacaagc ctaacaccag
     2581 taagaaaaaa gtttttttca tcaactaagc ccccttttta tctttcttat cattatagca
     2641 ttttttcgta agtaaaggct tacttatgtt gaaaaattac gcatttttcg ataaaatagg
     2701 agacagttat tttttaatag atcagaatag gagaaatcat gagaaaaata tacttatcta
     2761 ttttcacaag tctcttgctg atgctaggac ttgtcaatgt tgctcaagcc gatgaatatt
     2821 tacgcatcgg gatggaagca gcatatgctc cctttaactg gacccaggat gatgatagca
     2881 acggagctgt caaaatcgat gggaccaatc agtatgccaa cggatacgat gttcaaatcg
     2941 ccaagaaaat cgctaaggac ttaggtaaag aacctttggt tgttaaaacc aagtgggaag
     3001 gtctagtccc tgcccttact tctggtaaga ttgacatgat tatcgcaggt atgagtccaa
     3061 ctgcagaacg caaacaagaa attgcctttt cgagcagtta ctacactagc gaaccagttc
     3121 tactggtcaa aaaagattct gcctatgcaa gtgctaaatc tttggatgac tttaatggtg
     3181 cgaaaatcac ttctcaacaa ggggtctacc tttatgactt gattgcacaa atcacaggtg
     3241 ctaaaaaaga aacagccatg ggagacttcg ctcaaatgcg ccaagctctt gaggctggtg
     3301 tcatcgacgc ctatgtttct gaacgcccag aagctctgac tgccgaagcc gctaactcta
     3361 agttcaagat ggtccaagta gaaccaggtt tcaaaactgg ggaagaagat acagctatcg
     3421 ccattggact tcgtaaagat gacactcgta ttagccaaat caatgccagc attgaaacca
     3481 tttcaaagga cgaccaagtt gccctgatgg atcgtatgat caaggaacaa cctgccgaag
     3541 ctacaacaac tgaagagact agcagtagtt tctttagcca agttgctaaa attctttctg
     3601 aaaactggca acaactcttg cgtggtgctg gtatcactct tttaatctct atcgtcggaa
     3661 ccatcatagg tctcattatt ggacttgcca ttggtgtttt ccgtactgct cctctctctg
     3721 aaaacaaagc catttatggc cttcaaaaac tagtcggctg ggttctcaat gtctacattg
     3781 aaattttccg tggtacccca atgatcgttc aatcgatggt tatctactat ggaactgccc
     3841 aagctttcgg gatcaacctt gaccgtacac tggctgctat cttcatcgtt tcaatcaata
     3901 ccggtgccta catgactgaa atcgtccgtg gtggtatcct agcagttgac aagggacaat
     3961 ttgaagctgc gactgctctt ggtatgaccc ataaccagac catgcgtaag attgtcctac
     4021 ctcaggtagt ccgtaacatc ctacctgcaa ctggtaatga atttgtcatc aatatcaaag
     4081 atacatctgt attgaacgtt atctctgttg tcgaacttta tttctcagga aataccgtgg
     4141 caacacaaac ctatcaatac ttccagacat ttacaatcat cgccgtgatt tactttgtcc
     4201 tcaccttcac cgtaacacgt atcctacgat ttatcgagcg ccgaatggac atggatacct
     4261 atactacagg tgctaaccaa atgcaaacgg aggatttgaa ataatgacac aagcaatcct
     4321 tgaaattaaa cacctcaaaa aatcctatgg acaaaacgaa gtgctaaaag acatttcact
     4381 cactgtccac aagggagagg tcatctctat catcggaagc tctggaagcg gaaaatcgac
     4441 cttcctacgc tccattaacc tacttgaaac accaactgat ggacaaatcc tttatcatgg
     4501 acaaaacgtc ctcgaaaaag gctatgacct cacgcaatac cgtgaaaagt tggggatggt
     4561 tttccaatcc tttaacctct ttgaaaatct caatgttctt gaaaacacaa tcgtcgctca
     4621 gacaactgtc ctaaaacgcg aacgcacaga agctgaaaag attgccaaag aaaacctgga
     4681 aaaggtcggc atgggagaac gctactggca agccaaacca aaacaactct caggtggtca
     4741 aaaacaacgt gtggccatcg ctcgtgccct ctccatgaat ccggacgcta ttctctttga
     4801 tgaaccaaca tcagctctcg atccagaaat ggttggagaa gtcctcaaaa tcatgcagga
     4861 cctggctcag gaaggcttga ccatgattgt cgtaacccat gaaatggaat ttgcccgtga
     4921 tgtctctcac cgtgttatct ttatggataa gggcgtgatc gctgaagaag gtaaaccaga
     4981 agacctcttc accaatccta aagaagaccg aacaaaagag ttccttcaac gctatctcaa
     5041 ataaaaagaa aaggctgcat caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc
     5101 agattctcta tctctactac ttgaaggtca agaatccgcc gttatccaaa gattaatctt
     5161 caaatttttc ataatttttt tcatagaaat caattaggcc tagggctgtt tcaaatgaaa
     5221 tattttttac ttttgcacga ccctgcgcca gagcaatgat agacatttca cgcgcattcg
     5281 tttctttaga gatacggtag cctgtgattt tcttatcacg aacccagcca acaactgatt
     5341 ctactttttc aaagtttgac ttagccatgt ttttctccta ttttcttctt tataatattt
     5401 aattgtatac gtttttacta cttttgtcaa taaaaaaaac atatattcaa cgaaaataat
     5461 tagttcagaa ttttcttact tcctttttaa atccgataat atataggttt tactagcctc
     5521 tacaccataa atatttgcta ataaaaatac tgtattatta ccctcttaag gtgcgaaatt
     5581 attcaagttc ttaaaaaatg cttctctatt tccgtaagat agcttatttc ttcgatttaa
     5641 gaactgaaga tgatagttct aacctttaga aatccaagcc ttcactagct caaaaactcc
     5701 tatctttctt cctcaataaa tcatctgaga gataggtatt ccttatatcc ttcaaattca
     5761 tcactccttt ggatattgag gtgctagcct agaagcagac acccgattat ctaaaccatc
     5821 cttaataaag atgacaagac agataagctc agttaattac ttatatatcc ttttaatatg
     5881 gtatatttgt caacaaaaag aaatcaaatc ataccttata ctcaatgaaa atcaaagagc
     5941 aaactaggaa gctagccgca agttgctcaa aacactgttt tgaggttgca gatagagcta
     6001 acgtggtttg aagagatttt cgaagagtat taaagacagt gtgactatgc ttgtcaatag
     6061 ataggaaatc aaaatgaagg atggaaatct gagccaaaca tcataagtgc tataatgaac
     6121 agaagtaaga attgcacgat aagttttcta aaaccatagg aatcgtcccc tttcttcatc
     6181 ttcattatag cacatataca tcagttgtac aaataatatg atattttttt attagtcctc
     6241 agacaagcat attataaagc gtttcattac catttaagtt aatataagct ggatcaaaac
     6301 cttccattct acggatcaaa cctgcataat catgcttatc tgccaaagcg atcccaatca
     6361 atactgggcc tgttcccttg ctagctcgtt tgatatactc aaaacgtgtg atatcatcat
     6421 ttggccccag gatatcattt acaaactcac gcaaagctcc tggacgctgt gggaaattga
     6481 ccacaaagta atgtttgata ccatcataaa tcaaggcacg ctcttccatt tctggcatac
     6541 ggttgatatc attatttcct ccagaaatga tacaacaaat ggttttcccc ttaatatatt
     6601 cagctaaaac ctctaaagag gcgatactag ccgctccagc aggctctgcg actatccctt
     6661 gcttagagta aaggtcaatc aaggtttcag aaatcaatcc ctcatcgaca cctaccaaag
     6721 tttgaatatg ttgacgagtt gcttcatagg tcaactgacc taccttttgc acagcaatcc
     6781 catccgcaaa tttatcaatt tccttgagtt tgactggacc tccagcctca aaggcagctt
     6841 tcatggaacg cgctccattc gcctctactc cgataacctc aatctctgga cttgtttcct
     6901 tgatataggt agaaaccccg gcaatgagac cgccaccacc aacaggaacc aagacagcat
     6961 caaaatcaat cgattctttt cgagcttctt ctaaaatctc ataagcaact gttccttgac
     7021 ctgcttgaac atgagcatca tcaaaaggat caataaaggt acgattttca gagactgtaa
     7081 attcttgagc tgctttggct gaggcatcaa aggtatctcc aactagttta atagttacaa
     7141 aatccccacc aaaaaagcga acctgaccaa tcttttgttg tggcgtagta ataggcataa
     7201 agatagtagc aggaattttc atttcattac aagtataggc tactccctgc gcatgatttc
     7261 ccgcagaagc gcagactacc ccacgttcac gttcttcctt gctgagctgg gaaatggcat
     7321 aataggcacc acgaatttta aaggagcgaa cacgctgggc attttctttt ttcaaataaa
     7381 tcttagcacc atacttctcc gataaataat gatcgtaatc cagtggagta ttcacaacca
     7441 caccgttcaa gaccttgtga gccttgatta tatcttttga acttaacata taaaacctct
     7501 ttttctataa aaaacagtca ctaatgactg ttttttagtt attcatctcc gaaaacttca
     7561 gtaacacttt catggtattt atgagccata cgaatcgaaa actctttagg aagttcataa
     7621 cgatttgtgg aaatccaatc atataaccat tctaccaaac tttcttcact atcattacga
     7681 tggaagataa aatagtttaa aatttcttta tattctacat cattcaaatc caacgtgtct
     7741 acaaattgac tacgaggaac aaaattagaa tctaattttc ttagttgatc tgtcaattct
     7801 cccaattgta aatccaactg aaaaaatagt ttttgcagtt gcctatcagt ttcatcacat
     7861 cctgtcttga tagaaaacaa attggtaata ttcatatgat ttcaacccct aaataagtac
     7921 atctaatatc ccacgaatag ctgaagctaa agttttcgca gtccagtcat ttaaacccga
     7981 ttctttcaat ccagatgcaa taatttgctc tatttgatat aaaccgccat ctatcgtctc
     8041 atttattctg cgaagaggac cttcaatata ggataaagct tctccgaatc taacagctga
     8101 tttaggtcct aatgatttac caactatatt tgttaatgtt ttttatttct caaaatccat
     8161 ttcgctatat aacgagcaga tttagtcgca ccagctctct caaacggaga atgaatttca
     8221 agaccttctt ctctagctat ttgtgccaat ttgaagaatt gttcaaaggt catatgcaac
     8281 ctaccatctt caaaattagc atcatcataa atgttaatta cagcattttc atcatacgac
     8341 caatctgctt tattttggtc tgctagcact attaccgacg gtgtaattac attatacaac
     8401 tagaaccaat aataacgatg caatttttct cataatcttt ttcataaaat catctccagc
     8461 gctatatatt tctaattagt tatagatttt gaatgcatca tcgtcgtttt taccaacgaa
     8521 tggcattgct ttacgcaatt ctgcaccaac tttttcaatt tcaaggttag ctgcttgttc
     8581 acggtaagca gtcaattttg gacgtccagc tttatagtca tttacaaagt catttgcaaa
     8641 tttaccattt tgaatgtctg ccaagacagc cttcatattt tctttaactt gttcagtgat
     8701 tacacgtgga cctgatacat agtcaccgta ttcagcagtg tttgaaatag attgacgcat
     8761 tttcttgaat ccaccttcat agatcaagtc aacgatcaat ttcatttcgt gaagaacttc
     8821 aaagtaagcc aattctggag cgtaacctgc ttctgtcaag acttcgaaac ctgcttcgat
     8881 aagggcagtc aaaccaccac aaagtacagc ttgttcacca aacaaatctt cttcagtttc
     8941 ttctttgtaa gttgtttcaa gaagacctac acgagccgct ccaacacctt tacaccagtc
     9001 catagcaatg tttttagcat ttcctgttgc atcttggtat actgcataaa gagctggaac
     9061 accaaatcct tcttcgtaag tacgacgtac caagtgtcct ggtcctttag gagcacacat
     9121 gaagacatct acatccgcag gaactttgat aaattcaaag tggatgttga aaccatgggc
     9181 aaatccaact gcgtttccag cttccaagct tggagcgatt tctgcttcgt acaattcttg
     9241 ttgaatttcg tctggcgcca agatcatgat aacatcagcc aacttagtag cttctgctac
     9301 tgtgtaagta tcaaatccat cttcttttgc tttatcaaaa gatttacctg gacgtacacc
     9361 gataataacg tcacgacctg aatcacgcaa gttttgagca tgcgcatgcc cttgtgaacc
     9421 ataaccgata acggcgattt ttttaccgtc aagtgctgct actttaacat ctttttcata
     9481 ttccatttga actgtcatag cttttctctc ttttctatta tttattgcct tttaggctgg
     9541 tttaataaat ttaagttgga tttttaatcg cgggtaaatc cagttgcacc cgttcgagca
     9601 atattgcgaa taccgtatgg gcgaatgact cgcaataggg cttcgctctt ttctgcattt
     9661 cccgtcatct gaatggtaat cgagctcggc gctacgtcta ccactgttgc acggaaaggt
     9721 tgaataatcg ctaaaatctc agctctcttc tcagctggcg ctgacatctt aaccaaaatc
     9781 acctcgcgct ccaaatgagg cttgtctgta atatctcgaa tgcgaatcac atcaatctga
     9841 cgattgagct gtttgatgat ttgctccact tcatcatgag aagcaacatc aataataata
     9901 gtgatacgcg atacattcgg atcttctgtt gctccaacag agatgctttc aatattaacc
     9961 tgacgacgag ataggacacc tgtaaagcga ttgaggactc ctgaacgatt ttgtagtttt
    10021 gctgttaaca ttctacgcat ggaactgcac ccccaacatc tcatgattac tcttaccagc
    10081 cggtaccatt ggtaacacct gttccttacg agaaatatct acctcaatta gcataggaac
    10141 atcctcagtg atgacttcaa ggtcttgagc caaggtctca ggattgtcaa acttatagtt
    10201 tttaatacca taagcctgcg ccatcaattg gaaatcagga agggtatcaa agaccgactc
    10261 tgatgttctg ccttcataga aggattcctg ccactggcga accattccga gtgaatgatt
    10321 gttcagcata accaccttga ttggcacctt gtaaatattc aaaatagcca actcctggtt
    10381 ggtcatttgg aaaccaccat ccccaacaaa caagactact tccttatctg ggttagcaat
    10441 tttagcaccg attgctgctg gaattccaaa gcccattgtt cccaaaccac ctgaagtcac
    10501 taactgacgt tcattttggt agggataata ctgagctgtc cacatttggt gttgaccaac
    10561 gtctgttacc acaatggcat ctccattcgt caattcacca attcgttcaa taactgcttg
    10621 cggttgaacc acacgctctt tcttgtcata agaacgaaca cgattcttgt ctttagtgac
    10681 tttctcaatc cacttttcag tattgttgtg aactgttggt tctgctagca acatttgcaa
    10741 ggccttctta gcatctccaa ctacaggaat gtctgcactg ataatcttgc caatctcagc
    10801 tgggtcaata tcaatgtggg caaccttagc attcttagcg aaagtcttag gattccccgt
    10861 caaacggtca tcgaaacgag aaccaatact aatcataaag tccgcttccg tcatggcaat
    10921 atttgctgcg aatgacccgt gcatgcctcc cattccaaga aagagtgggt gactcgttgc
    10981 aatcgttcct tgtcccaaaa gactggttac cactggaatt tgatagcgtt ctgcaaattc
    11041 atttagttcc gtagcagcct cagcataact aattccacca ccagctaaca agactggctt
    11101 tttagccttg gacaattgct tcaagatttt cttgatttgc atatcattcg gctcaagagt
    11161 cggctgataa cttggtaaat tcacttctgg tgaataaatg aagtctgttt ctaaagcaga
    11221 tatgtctttt ggtaggtcaa ttacaactgg ccctggacgg cctgtagttg cgatatggac
    11281 agcttccgta atgatacgcg gaatatcagc tgtctcacga acttggtaat tgtacttagt
    11341 gattggcatg gtaattccca cgatgtctgc ctcctgaaag gcatccttcc caatccctgc
    11401 tcgcgccacc tgacctgtaa agaccaaaag gggaacgcta tcgctcatgg catccgcaat
    11461 ccctgtaatg gcatttgttg ctcctggtcc actagtgacg acggcaacac ccaactttcc
    11521 agttgatttg gcataacctt cagcttcatg caaacaacct tgctcatggc gccctagaat
    11581 gtggcgaatg cctttaaaat tatatatcgc atcataaaaa ggcaaaaccg caccaccagg
    11641 ataaccaaag atggtatcaa ctcctaaatc acgaagtgtt tccaaaacta ggtccgaccc
    11701 cgtcttagga gattctaaac tgattttctc cattgttccc ctttctttcc tcttaaaaat
    11761 aacttgttac tatcatacca ttttttcaaa atttttcaag ataaaagaag aaattttctg
    11821 aattttctat tttaacgttt atttatgaat gttatttttg tttttattaa aaagataccg
    11881 atttcattta ctaaaaaaga aaaaagaact gatttctcag tccttcatta atcttattcc
    11941 acactaaata ggtatgggta aacaggttgt tgaccttggt gaatctcgac ttcaacgtct
    12001 tcgaattctt ctacgatttc ttgagcgatt tcattggcaa gttcttcgct tccgtcttca
    12061 ccgacataga aggttacgat ttcactgtct tcatccaaca tatgtttcaa ggtttcagtc
    12121 aatgtttggt gcatatcagg gtttgacaca agaatttttc catccaccat acctagatta
    12181 tcgttttcat ggatttctaa gccatcgatc gttgtatcac gcacggctgt tgtgacgctt
    12241 ccgctaacga catctctaag agcagctgtc atactctctt gattttcttc aatggacttg
    12301 cttggatcaa aggcaagaag acttgtcata ccttgaggaa gagtgcgagc ctctaccact
    12361 actgctggtt gctccaaaac ttctgccgca gattgagctg ccatgaagat gttcttgttg
    12421 tttggcaaga agatgatgtt acgggcattg acctgttcaa cagccttgat aaagtcttct
    12481 gttgaagggt tcatggtttg accgccttcg ataacataat ccacgccttg agaacagaag
    12541 atatctgcta gacctttacc agccaccaca gcaatcaaag catactcttt ttcttcagcc
    12601 gacttgataa cttgagtagc ttctttctca acctgtgctt cgtgttggtt acgcatattg
    12661 tcaactttta ccttgaccaa gctaccatat ttgagacctt cttgcataac aagtcctgga
    12721 tcttctgtat gaacatggac tttgacaatt tcatcatcgt taacaacgag gagagaatcc
    12781 ccaagctcat ccaagtagtt acggaattca tcgtagtcaa aatctttagc ataggttgga
    12841 ccttgcttaa gagctaccat gatttcagta cagtaaccaa acgtgatgtc ctcagtcgct
    12901 acgtgaccag ctacagactt atgatgctct acattgatca tctcactcat gttggcagga
    12961 gtcgctacaa agtcctcaga tgcaatatat tcgccagtaa gggctgaaag gaaaccttcg
    13021 tagatgaaga ccaatccttg accacctgag tccacaacgc caacttcttt caatactgga
    13081 agcatgtctg gtgttttagc tagagctgtt ttagcacctt ccaaggctgc gcgcatgact
    13141 tcaacagtgt catctgtttg ctcagctttt ttcttagcac cgatagcagc tccacgagaa
    13201 actgtcaaaa tcgttccttc aacaggtttc atcactgcct tataggcaac ttccacacct
    13261 gattggaagg ccagagccaa gtcttgacct gttaactcgt ctttatcctt gatagcttgg
    13321 gaaaatccac ggaaaagctg agacgtaatc actcctgagt tcccacgcgc acccatcaaa
    13381 agccctttgg caagaatgct cgctacttct ccaactgtag aagctggctt gtctgcaact
    13441 tctttagcac cattttcaat ggtcattccc atatttgtcc cagtatctcc atctggaact
    13501 ggaaagacgt ttaatgaatt gacatattca gcttgcttat tcaagcgagt tgatgcagcc
    13561 tgcaccattt cttgaaataa gctagtagta atttttgaca cggttattct cctacaactt
    13621 tgatattttg aatgtagaca tttacagtct gagcagtaat tccaagctgg ttttccaagc
    13681 taaaggcaac acgctcttga atgttttttg acacttcact aatctttgtt ccgtagctta
    13741 acacggtata tacatcaact gcaatactgc catcttcggc tgcctttacg acgacaccct
    13801 tagaataatt ttctttacct agcagggctt ggaaattatc tttgagggca tttttactag
    13861 ccataccgac cacaccaaaa atctcagttg ctgctccacc tacgacggtt gcaatcactt
    13921 catctgttag ttcgatttga ccatcttttg tattaatttt tacagtcatc ctttttacct
    13981 caactagttg atactctatt ttatcatatt tcagcccaag tgtaaaagca gaatgatgta
    14041 tcagcggata tttactctat ttttcaaacg atgttatacc cacaataaaa gaaaaaagac
    14101 cctaaggtct cctttgcttt tattattaaa cgcgttcaac tttacctgat ttcaaagcac
    14161 gagctgaagc ccaaactttt ttaggtttac catcgataag aacagtaact ttttgaaggt
    14221 ttggttttac ggcacgtttt gtttggttca tcgcgtgtga acggttgttt cctgatacag
    14281 tcttacgacc tgtaaagtaa catactttag ccattgtgtt ttcctcctat tagatctaat
    14341 atagcggatg tgctagcacc acataccgta ctatgttatc acactttctt gttttttgca
    14401 agggaattgg aagatttttt atttgtgtct taaatcaggt cttgcgtgat attttctgct
    14461 ctccacatgc cattgttgat taacagaaca ccagaattaa aattatgtgt ataaaaatca
    14521 tctctaactg cagctaaggg tatagccgtc aagtccaaat cccacagctc atctatcgat
    14581 tttcttacaa caatatctga atccaaatac agtacacgag actcgcttac atactttgga
    14641 ataaaatacc taaaaaagcc gtatatgaaa gtccctcaaa ggggagacga taacctttca
    14701 gaatactact gtcaatctaa acattcacaa tctcactatt caaagtctct agtctttttt
    14761 ccatcaattg gaaccattct cgcggaaggt catcattaaa aacataaaac ttaagattat
    14821 aatgatgaac acaaagagat tttattgttg tttcaacttt atccatataa gcattatctg
    14881 cacctaagac aatcgctttt ttctcttctt tcacttttta tctcatttct ttttattccc
    14941 atcatatgtt tctacgtaac cattattttc gcccattcgt tcgtaaaacc ataccagtgg
    15001 agattttaga tgaagtccca ttacggttta caatttttac attacgacac ggagttttac
    15061 aaatcgattt catttgccaa acgtagttag taaggcagtt agctaggcta cctactttct
    15121 gctacgacgg aaaaatccac aaatcaatac cattcgtgga tttttcctag tgaagcgttt
    15181 aggtaagccg ccatagcggt taccgattat tagattacga cacggagttt ggcaaatcga
    15241 ttctatttgc caaacgtagt tagtaaggca gttagctagt tcaccaaata gcgactagcg
    15301 tccaacaatt tggaacttta gttccaattg ttggtactga gtcacatctt ctcctctaac
    15361 tctacgtctg gatacttgtc cgcaaaccag cggagggcaa agtcattttc aaagagaaag
    15421 actggttggt caaaacggtc tttggctaag atattgcgac ttgacgacat ccgttcatcc
    15481 aagtcctcag gcttaatcca acgaacggtc tttttaccca ttggggtcat aactacttcc
    15541 gcattgtact cgccttccat gcggtgttta aagacttcaa actggagttg acctacagcg
    15601 cctagcatgt actcacctgt ttggtaattc ttataaagct gaacggctcc ttcttgcacc
    15661 aattgctcaa tccccttgtg gaaggatttt tgcttcataa cattcttagc agaaactttc
    15721 atgaaaattt caggagtaaa ggttggcagg ggttcaaatt caaacttgtt ttttccaacc
    15781 gtcaaggtat ccccaacctg ataagtaccg gtatcgtaaa ccccgataat atcacctgcc
    15841 acggcattgg tcacattctc acgactctcc gccataaact gggtaacatt agatagtttg
    15901 gcccccttac cagtacgagg aagattgaca ctcatgccac gctcaaattc gcctgatacg
    15961 atacggacaa aggcaatacg gtcacggtga cgaggatcca tgttggcttg gattttaaag
    16021 acaaagcctg agaaatcctt gtcataagga tccacaattt caccgtctgt tttcttgtga
    16081 ccatgtggtt ctggagcaaa cttgaggaag gtttcaagga aggtctgcac accaaagttt
    16141 gtcagggctg aaccgaaaaa gacaggcgtc aattctccag ccagaatagc ttcctctgac
    16201 aactcattcc cggcttcatt taaaagctca atgtcatcct tgacttgctc gtagaaagga
    16261 ttgctaccaa agagtttgtc cccgtcttct agactggcaa aacgctcatc ccctttgtaa
    16321 agctctaaac gttggttata gaggtcatac aagccctcaa aggctttccc catcccgata
    16381 ggccagttca tagggtagct agcaatgccc aagatttctt ccaattcttg caagagatcc
    16441 aaaggctcac gaccgtcacg gtccagcttg ttcataaagg taaagactgg aatgccacga
    16501 tgtttcacaa cctcaaacaa tttcttggtt tgagcctcga tccccttggc agagtccacg
    16561 accatgaccg cagcatccac cgccatcaag gtacgatagg tatcttctga gaagtcctcg
    16621 tgccctggcg tgtctaagat attcacgcgc ttgccgtcgt agtcaaattg cataacagat
    16681 gaagtaacag aaatcccacg ttgcttctcg atatccatcc agtcagattt agcaaaagtc
    16741 cctgttttct tcccttttac cgtaccagcc tcacgaatct cacccccaaa gtagagtaac
    16801 tgctcagtga tggttgtttt ccccgcgtcc gggtgggaga taatggcaaa ggtacgacgt
    16861 ttcttaattt cttcttgaat attcataagt tctctttctt tgattctcta tttttcttgt
    16921 ttcaatagct gagaatgatt tttacattgg attttaccat tcctttcaac actccattat
    16981 atcggatttt agcatttttt tcaatttcta tttcttttca cttccccctc ccttatttat
    17041 aggaaaatat ggtaaaatag aacagactaa aaatcatcat ttcacgaaag gatgcaagat
    17101 gaaaattacg caagaagagg taacacacgt tgccaatctt tcaaaattaa gattctctga
    17161 aaaagaaact gctgcctttg cgaccacctt gtctaagatt gttgacatgg ttgaattgct
    17221 gggcgaagtt gacacaactg gtgtcgcacc tactacgact atggctgacc gcaagactgt
    17281 actccgccct gatgtggccg aagaaggaac agaccgtgat cgcttgttta aaaacgtacc
    17341 tgaaaaagac aactactata tcaaggtgcc agctatccta gacgatggag gagatgccta
    17401 atgactttta acaataaaac tattgaagag ttgcacaatc tccttgtctc taaggaaatt
    17461 tctgcaacag aattgaccca agcaacactt gaaaatatca agtctcgtga ggaagccctc
    17521 aattcatttg tcaccatcgc tgaggagcaa gctcttgttc aagctaaagc cattgatgaa
    17581 gctggaattg atgctgacaa tgtcctttca ggaattccac ttgctgttaa ggataacatc
    17641 tctacagacg gtattctcac aactgctgcc tcaaaaatgc tctacaacta tgagccaatc
    17701 tttgatgcga cagctgttgc caatgcaaaa accaagggca tgattgtcgt tggaaagacc
    17761 aacatggacg aatttgctat gggtggttca ggtgaaactt cacactacgg agcaactaaa
    17821 aacgcttggg accacagcaa ggttcctggt gggtcatcaa gtggttctgc cgcagctgta
    17881 gcctcaggac aagttcgctt gtcacttggt tctgatactg gtggttccat ccgccaacct
    17941 gctgccttca acggaatcgt tggtctcaaa ccaacctacg gaacagtttc acgtttcggt
    18001 ctgattgcct ttggcagctc attagaccag attggacctt ttgctcctac tgttaaggaa
    18061 aatgccctct tgctcaacgc tattgccagc gaagatgcta aagactctac ttctgctcct
    18121 gtccgcatcg ccgactttac ttcaaaaatc ggccaagaca tcaagggtat gaaaatcgct
    18181 ttgcctaagg aatacctcgg tgaaggaatt aacccagagg ttaaggaaac cattcaaagt
    18241 gccgctaaac actttgaaaa acttggtgct attgtcgaag aagtcagcct tcctcactct
    18301 aaatacggag ttgccgtata ctacatcatc gcttcatcag aagcttcatc aaacttgcaa
    18361 cgctttgacg gtatccgtta cggctatcgc gcagaagatg caaccaacct tgatgaaatc
    18421 tatgtaaaca gccgaagcca aggctttggt gaagaggtaa aacgtcgtat catgctgggt
    18481 actttcagtc tttcatcagg ttactatgat gcctactaca aaaaggctgg tcaagtccgt
    18541 accctcatca ttcaagattt cgaaaaagtc ttcgcggatt acgatttgat tttgggtcca
    18601 actgctccaa gtattgccta tgacttggac tcactcaacc atgacccagt tgccatgtac
    18661 ttggctgacc tcttgaccat acctgtaaac ttagcaggtc tccctggaat ttcgattcct
    18721 gctggattct ctcaaggtct acctgtcggt ctgcaattga ttggtcccaa gtactctgag
    18781 gaaaccattt accaagctgc tgctgctttt gaagcaacaa cagactacca caaacaacaa
    18841 cccgtgattt ttggaggtga caactaatga actttgaaac agtcatcgga cttgaagtcc
    18901 acgtagagct caacaccaat tcaaaaatct tctcacctac ttctgcccac tttggaaatg
    18961 accaaaatgc caacactaac gtgattgact ggtctttccc aggagttcta ccagttctca
    19021 ataaaggggt tgttgatgcc ggtatcaagg ctgctcttgc cctcaacatg gacatccaca
    19081 aaaagatgca ctttgaccgc aagaactact tctatcctga taaccccaaa gcctaccaaa
    19141 tttctcagtt tgatgagcca atcggttata acggctggat tgaagtcgag ctagaagacg
    19201 gtacgaccaa gaaaatcggt atcgaacgtg cccacctaga ggaagacgct ggtaaaaaca
    19261 cccatggtac agatggctac tcttatgttg acctcaaccg ccaaggggtt cccttgattg
    19321 agattgtatc tgaggcagat atgcgttctc ctgaagaagc ctatgcttat ctgacagccc
    19381 tcaaggaagt tatccagtac gctggcattt ctgacgttaa gatggaggaa ggttcgatgc
    19441 gtgtggatgc caacatctcc cttcgtcctt atggtcaaga gaaattcggt accaagactg
    19501 aattgaagaa cctcaactcc ttctcaaacg ttcgcaaggg gcttgaatac gaagtccaac
    19561 gtcaagctga aatacttcgc tcaggtggtc aaatccgcca agaaacacgc cgttacgatg
    19621 aagctaacaa agcaaccatc ctcatgcgtg tcaaggaagg ggctgctgac taccgctact
    19681 tcccagaacc agacctgcct ctctttgaaa tctcaaacga gtggattgag gaaatgcgga
    19741 ctgagttgcc agagtttcca aaagaacgtc gtgcgcgtta tgtatctgac cttggtttat
    19801 cagactacga tgctagtcag ttgactgcta ataaagtcac ttctgacttc tttgaaaaag
    19861 ctgttgccct aggtggtgat gccaaacaag tctctaactg gctccaaggg gaagtcgctc
    19921 agttcttgaa tgctgaaggt aaaacactgg aacaaatcga attgacacca gaaaacttgg
    19981 ttgaaatgat tgccatcatc gaagacggta ctatttcatc taagattgcc aagaaagtct
    20041 ttgtccatct agctaaaaat ggcggtggcg cgcgtgaata cgtggaaaaa gcaggtatgg
    20101 ttcaaatttc agatccagct atcttgatcc caatcatcca ccaagtcttt gccgataacg
    20161 aagctgctgt tgccgacttc aagtcaggca aacgtaacgc cgacaaggcc tttacaggat
    20221 tccttatgaa ggcaaccaaa ggccaagcca acccacaagt tgcccttaaa ctacttgcac
    20281 aggaattggc gaagttgaaa gaaaactaga cagaacaaaa ccagccctaa ggttggtttt
    20341 ttcttctcta ccaactccca ataactattt tggctttatt tccagagtat tttatggtaa
    20401 aatgaagagt aataatattt attaaagagg taaaaacatg attgaagcaa gtaaattaaa
    20461 agctggtatg acctttgaaa cagctgacgg caaattgatt cgcgttttgg aagctagtca
    20521 ccacaaacca ggtaaaggaa acacgatcat gcgtatgaaa ttgcgtgatg tccgtactgg
    20581 ttctacattt gacacaagct accgtccaga ggaaaaattt gaacaagcta ttatcgagac
    20641 tgtcccagct caatacttgt acaaaatgga tgacacagca tacttcatga atacagaaac
    20701 ttatgaccaa tacgaaatcc ctgtagtcaa tgttgaaaac gaattgcttt acatccttga
    20761 aaactctgat gtgaaaatcc aattctacgg aactgaagtg atcggtgtca ccgttcctac
    20821 tactgttgag ttgacagttg ctgaaactca accatctatc aaaggtgcta ctgttacagg
    20881 ttctggtaaa ccagcaacga tggaaactgg acttgtcgta aacgttccag acttcatcga
    20941 agcaggacaa aaactcgtta tcaacactgc agaaggaact tacgtttctc gtgcctaatc
    21001 tctagaaaga ggtcattcta tgggaattga agaacaactt ggcgaaatcg ttatcgcccc
    21061 acgtgtactt gaaaaaatca ttgctatcgc tactgcaaag gtagagggtg ttcactcttt
    21121 ttcaaacaga tcagtgtctg ataccctttc aaaactttca ctcggccgtg gcatttatct
    21181 taaaaacgtg gacgaagaac tcacagcaga tatctatctc taccttgagt acggagtaaa
    21241 agttcctaag gtagcggttg ctatccagaa agctgtcaaa gatgccgtcc gtaatatggc
    21301 tgatgtagaa ctcgctgcta tcaatattca cgttgcaggt atcgtcccag ataaaacacc
    21361 aaaaccagaa ttgaaagatc tatttgacga ggacttcctc aatgactagt ccactattag
    21421 aatctagacg ccaactccgt aaatgcgctt ttcaagctct cataagcctt gagttcggta
    21481 cggatgtcga aactgcttgt cgtttcgcct atactcatga tcgtgaagat acggatgtac
    21541 aacttccagc ctttttgata gacctcgttt ctggtgttca agctaaaaag gaagaactag
    21601 ataagcaaat cactcagcat ttaaaagcag gttggaccat tgaacgctta acgctcgtgg
    21661 agagaaacct ccttcgcttg ggagtctttg aaatcacttc atttgacact cctcagctgg
    21721 ttgctgttaa tgaagctatc gagcttgcaa aggacttctc cgatcaaaaa tctgcccgtt
    21781 ttatcaatgg actgctcagc cagtttgtaa cagaagaaca ataaaaagaa aaagtgtttg
    21841 atagatatca aacacttttt tctttgcctc ccactatcta aaaaaatgat aatagatata
    21901 attgtaaaca aaaatccaga taggttttgc atgattgaga aagttaaaaa aactatggca
    21961 gagaatcgtt aatctcagat tgtcggtaga acgataaaca agggcaaaaa agaaaccaat
    22021 cagactataa tataataaac taattggatc tctgtgagat agtatcaaat ggctaatccc
    22081 aaagatgata gcagatagga taacatccaa ataatacttg gactagggaa agaaggtgtt
    22141 cataaaatac cctctatcaa gagtctcctc aaaaacagga ccgatgatta caggcaggac
    22201 aaaagataag atagtcgata aaaaggttgg ttgtccattt gaaagaacca cggtaaaata
    22261 ctcatcatga atattcctat gattaatcaa atgagcatag cgtgcccaaa aattaccgag
    22321 aatctgataa accacataag ttgcaaataa ggagaagaca aatgaccagt cccagctctt
    22381 tttctttttt aacctccaaa ttaatagaaa aggaaacttc ccactaatcc cactgttaaa
    22441 ataagagaat agacatcagc tcctaaccct aaaatgatcg tcacatacaa tccaattgtt
    22501 tgtggtaaat aggtagatag taaaataata agcaaaaata ttccaaattg tcttagtttc
    22561 tttgtgtttc tcatcgtact tttttgaaag attaccctgt tcggaagccg tacttccaag
    22621 catctatata agaattaagt gccccttgcc tcatataggg agcaaattct ctataatata
    22681 accatctact atatccatct tcccaaacag caagaccacc tgaagtttgc tccaagtcct
    22741 cagttgaaat aactgtaaat gtttctgtac ctgtcattgc aagtaccttc ttaaaataga
    22801 ttgttgtagg ctcacattta tagtatattt cttttttgtc tattttatag cccatctcct
    22861 caactatcaa tttttcaacc tgaattgcat ttttccataa aaaatgagac ctttctagtc
    22921 tcatttagtc attcttagta ttttctaaat cgttgatagc gttcttccag caactcttct
    22981 agcggttttt gtgaaagtct agccagctcc gtttggagtt cttttttgac actcttaatc
    23041 agttctttac tagaaagtcc tacttcagaa atcaccttat ccaccacgtc catttctaac
    23101 agttcatgcg aagtgatttt catcagttct gctgcttcca tggcgcgagt accgtccttc
    23161 cataaaatgg aagcaaagcc ttctggactg agaatggcat agatagaatt ttccagcatc
    23221 cagacacggt ccgcgacagc tagagccaga gccccgcctg aaccaccttc accgataata
    23281 atggcgataa taggaacttt caggtcactc atttccatga gattgcgagc gatagcttcc
    23341 ccttgaccac gttcttctgc tccgacacca ggataagcac ctgctgtatt aataaaggtc
    23401 acaactggac ggccaaattt ctcagcctgt ttcatcaacc gcagtgcctt tcggtagcct
    23461 tctggatgtg gttggccaaa attccgtttg aggttgtctt gcaaactctt gcctttttgg
    23521 ataccaacca ctgttacaac ttggtctcca agccaaccaa taccaccaac aactgcacca
    23581 tcatcacgaa aagaacggtc accatgtaat tggataaatt catcaaaaat gcctgtcgca
    23641 aagtccaagg ttgtcaagcg actctgctca cgcgcttctc tgactatttt tgcaatattc
    23701 atctaggact ccctccatgc aatctgacta ggctagcaat cgtatctggt aagtctcttc
    23761 ttttgacaat agcatccaca aagccatgtt ctaataggaa ttctgccttt tggaaatcct
    23821 caggcaagct ttcacgaacc gtattttcaa tcacacgacg cccagcaaaa ccaaccaagc
    23881 tctgtggttc agccagaatg atatcgcctt ccatagcgaa agaagctgtc acaccaccag
    23941 tcgttggatc tgtcaaaatg gtcaggtaaa agagaccagc atttgaatga cgtttaaccg
    24001 ccgcagagat cttagccatc tgcatgagac tcatgattcc ttcctgcata cgggctccac
    24061 cagaggctgt gaataggaca actggcaatt tttcgacagt cgcatactca aacaaacgag
    24121 tgattttttc acctacaacc gtacccatag aagccatgat aaagttagaa tccataatcc
    24181 caagagccac agtctgacct ttaataagag cagttcctgt cacaacggct tcatgcagac
    24241 ctgttttttc acgcatagat gccagtttct tttggtaacc agggaaatgc aagggatcct
    24301 tgctttcaat ccctgtaaac aattctttga aggttcccat atcaatcgtc aaagccaagc
    24361 gttcttgggc agaaatacga aaggtatagc tacagtgcgg acagatacgt tcacttccca
    24421 gatccttctg atagatggta tgcttacagc ctggacactg ggaaaataat tcatctggaa
    24481 cctctggctt agcttgaggt ttttccctaa ccgaacgatt gggattgatt cgaatatact
    24541 tatctttttt actaaataga gccattgatt ccccttttcg gtttaaactc ttaaagtcat
    24601 tttattcttt ttcttgatat ttaggtaaga aggtttccat caagaaggaa gtatcataat
    24661 ccccagcaat gacattgcga tctgaaatga ggtcaagctg gaaatctgca ttggtctgca
    24721 ctccttcaat ctctaattca tagagggcac gttgcatttt catcaaggcg tcaaaacgat
    24781 tttcgccgtg tactatgatt ttggcaatca tactatcata ataaggcgga atggtataac
    24841 ctggataaac tgctgaatcc acgcgcaagc caactccacc acttggcaga tagagattag
    24901 taatcttacc tggacttgga gcaaagttaa aggctgggtt ttctgcattg atacgacact
    24961 cgatggcatg accgcgtagg acaatatctt cttgcttaac agacaaaggc tgacctgccg
    25021 caatgcaaat ctgttcctta acgatatcaa cacctgaaac aaactctgtt actggatgtt
    25081 ctacctgaac acgagtattc atctccatga aatagaaatt gctacttgct tcatcaagaa
    25141 gaaattcaat ggttcctgca ttctcatagc caacaaactc tgccgctcga acagcagcag
    25201 cacctatttc atgacgcagc gtttttccga ttgcaatcga gggactttct tccaaaacct
    25261 tttggttatt cctttgaaga gaacaatccc gttcacccaa gtgaatcaca tgtccatgct
    25321 catcacctag gatttgaacc tcaatgtgcc gagctggata gataacccgt tctatgtaca
    25381 tggcaccatt gccataattg gccttggcct cactagaggc agtttcaaag gcagaaacga
    25441 ggtcatctgg ttttttaacc ttacgaatcc ctttaccacc tccacctgct gaagccttga
    25501 gcataacagg atagccaatt ttttcagcaa caatcaaagc ttcttcagaa ttatgcactt
    25561 ctccatctga acctggtata acaggcacac ctgctttaat catctgagca cgcgcattga
    25621 tcttatcccc catcatatcc ataacatgac cagatggacc gataaacttg atacctactt
    25681 cttcacacat ggtcgcaaat ttggaatttt cactgagaaa tccaaaacca gggtgaatag
    25741 cttctgcctc agtcaagact gcagctgata gaactgcatt aatattgaga taagactctg
    25801 ttgccttgcc aggaccaata caaactgctt catctgccaa aagcgtatga agagcttcct
    25861 tatcagcagt tgaataaacc gctaccgtcg caatccccaa ttcacgtgcc gcacggataa
    25921 tacgaaccgc aatttcacca cgattggcaa ttaaaatttt tcgaaacatg gagaacctcc
    25981 ttagttccca attgcaaaag taaggatacc actggctgca agcttgccat ccacttcagc
    26041 ctttgcttca accacagcta tggtgccacg acgttttaca aaagtcgctg tcataaccaa
    26101 ttggtcgcct ggtacaactt gcttcttgaa cttaaccttg tccataccag cgtaaaagac
    26161 cagttttcct ttattttcag gttttgataa ctccaacaca ccagcagttt gcgccaaggc
    26221 ttccataatc agaacacctg gcataactgg gtattgagga aagtggccgt taaagaaagg
    26281 ttcgttaatg gtcacatttt tgatagcaac aatggtatcc tcgctcactt ccaagacacg
    26341 gtccactaga agcataggat aacggtgggg aagagcttct ttgattcctt gaatatcgat
    26401 catttgatac gtaccaatcc tttaccaaac tcaaccattt cttcgttaga gacgagaatt
    26461 tccgttacca caccatcctt aggagctggg atttcattca tgactttcat ggcttcgata
    26521 attaccaatg tttgaccttt tttgacacta tcaccaactg taacgaaggc aggtttatct
    26581 ggtccagcag ccaagtaaac cactccaaca agtggactct ctacaagatt tccctcagta
    26641 gccacacttg cttcagctgg agctggaact tcttctgcta cagtctctgc tggagcagat
    26701 gtaggagcta ctggactcgg tgttgctaga acgggtgctg gagcgacttg agttgcaact
    26761 tcaggcacag gtcttgcttc attcttgcta aactgcaact catccgtccc atttttataa
    26821 gaaaattctc tcaaacttga ctggtcaaat tgagtcatca agtctttaat atcgtttaaa
    26881 ttcatactta tctattctcc caacgtttga aagcaagaac tgcattgtgg cctccaaaac
    26941 caaaagtatt tgaaatagcg tatggaattt ctttctccaa gccttgtcca taaacgacat
    27001 tagcttcgat ataatctgat acttcacttg tcccagctgt cattggtaca aagttatgac
    27061 gcatagcttc gatggtgacg atagcttcta ctgcacccgc agcccccagc aaatgtcctg
    27121 taaaagactt ggttgatgat acaggtactt ccttaccaag aacagctacg atagctccac
    27181 tttctccttt ttcattggca ggagttgacg ttccgtgagc attgacatag gctacttgct
    27241 ctggagaaat ctcagcttct tccaaggcta gtttgatggc cttgatagct ccctgacctt
    27301 ctggatgtgg agaagtcatg tggtaggcat cacaagtatt tccgtaacca accacttcag
    27361 ccaggatagt agctccacgt ttttcagcgt gttcaaggct ttctagaacc aacatccctg
    27421 aaccttcacc cataacaaac ccattgcgat ccttatcaaa tgggatcgaa gcacgagttg
    27481 gatcctctgt agtagagaga gctgttaagg cttggaaacc agcgatggca aaaggtgtga
    27541 tagaagcttc tgttcctccc accaacatca catcttggaa accaaactta atggagcgga
    27601 aggcatcccc aatcgcatca tttgatgaag agcaggcagt attgatagat ttacaaacac
    27661 cgtttgcacc aaaacgcatg gctacattcc cagaagccat atttggtaaa gcttttggaa
    27721 gagtcattgg tttgacacgt ttgggtcctt tttcatgaag gcgaagtacc tgatcttcaa
    27781 tttccttgat tccaccaata ccagatgcaa cgataacacc aaaacgatcc ctattaagag
    27841 cctctacatc aagattggca tgatttacag cctcttgggc tgcatacaag gcatataaag
    27901 aatagttatc aaaacggttg gtatcttttt ttataaagta tttatcgaac ggaaaatctt
    27961 ggatttctgc cgcattatgc acatcaaagt cactatgatc aaattttgta atgccaccaa
    28021 tgccgatttt cccagttgct aaactattcc aaaattcttc tggtgtattt ccaattggag
    28081 atgttactcc ataacctgtt accactactc gatttagttt cattcttttc acctctagct
    28141 ttcgctacat acttaagcca ccatcaatgg caatcacttg accagttaga taatcttggc
    28201 ctgctaaaaa tactgtcaaa tctgcaacct gctctgcctg cccaaattct ttcatcggaa
    28261 tctgagctag tgtagcttcc ttaatcttat ctgataagat agcggtcata tcagactcaa
    28321 tcattcctgg agcaatcaca ttgactcgta tattccgact agcgacttcg cgtgccacag
    28381 acttggtaaa gccaatcaag ccagccttag aagcagcata gttagcttga ccaatattcc
    28441 ccatcaaacc aacaacacta gacatattaa tgatagcacc ttctctggct ttcatcatcg
    28501 gtttcaagac tgattgtgtc atattaaagg caccagtcag attgaccttg agcacttttt
    28561 caaaatctgc ttctgtcatc ttaagcataa gagtatcttg ggtgatccct gcattgttga
    28621 ccaaaacatc tactgaaccc agttctgcaa tagcttgatc aatcatacgc ttagcgtctg
    28681 caaaatctga tacatctcct gaaatgggaa ccaccttgat accatagttt gaaaactcag
    28741 cgagcaattc ttctgagatt gccccacgac tgtttaagac aatgttggct cctacttgag
    28801 caaacttgtg ggcgatggca agaccaattc cacgactcga acctgtaata aagatatttt
    28861 tatgttctag tttcattttt ttcctttcaa aacttctact tattttagcc tatttctcta
    28921 aaagtgctac taaactcgct tgatcttcca catgagctaa gtgagcagtt tgatcaattt
    28981 ttttaacaaa gcctgacaag actttccccg gtccaatctc gataaagttg cttatgcctg
    29041 cttcttgcat gaccccaata ctttcataga aacgaacggg ttccttgacc tgacgcgtca
    29101 agagctgagc aatgtcctct ttttgcatca cagcagcttc tgtattgccg actaggggac
    29161 aagtaaaatc tgaaaaactt acctgagcta gagtttcagc tagtttctgg ctagcaggct
    29221 caaggagagc ggtgtgaaag ggacctgaca ccttaagagg aatcaagcgt ttggcacctg
    29281 cttcttgcaa aagttcaacc gctcgatcaa ctgcaaccac ttctccagca atgacgattt
    29341 gtgcaggtgt gttatagttg gctggagtaa ccactccaag ttcagaagct ttttgacagg
    29401 cttcttcaat gacctctact ggcgtattga gaactgctac catcttgcca gagtcagcag
    29461 gagccgcttc ttccatatag gctccacgct tagctaccaa ggcaaccgca tcttcaaaat
    29521 ccaaggcgcc gcttgccacc aaggcagagt attctccaag agacaaacca gcgaccatat
    29581 caggctgata gcccttttct tgcaataaac ggtagatagc aaccgaagtc gctagaatgg
    29641 ctggttgcgt atagcgggtc tgattgagtt tgtcctcttc cgtatcgatg agataacgca
    29701 aatcataacc gagcacctga ctcgctcgat caatcgtttc tttaacaatc ggatactgat
    29761 catagaaatc ccgtcccatc cctagatact gggcaccttg accagcaaat aaaaaggctg
    29821 ttttagtcat ttcttacaac tcctgtccag cgagaggctt cttcttgaat tttcttagcg
    29881 gctccgtaat acaaatcttt taggatttct tcagctgttt cttctttaga aacaagccct
    29941 gcgatttgac ctgccatgac agagccacca tccacatcac cgtgaacaac tgctttggct
    30001 agggcacctg ctcccatttg ttcaaagatt tctaaatcag gatcttcctg cttaaaggca
    30061 tctttttcag ccagttcaaa atctctagtc aactgatttt taatagcacg aacagcatga
    30121 ccaaagtgct gagctgaaat cgtagtgtca atatcccttg cttttaaaat tttctccttg
    30181 tagtttggat gggcattcga ctcttttgca actacaaacc gtgtccccac ctgtacagcc
    30241 tctgcaccta gcataaagcc agccgcagca ccttcaccat ccgcaattcc tcctgcagca
    30301 ataacaggaa tagatatagc tgtggctacc tgtcgcacca aggtcatggt tgttaattta
    30361 ccgatatgcc ccccagcttc cattccttct gcaataacag cgtctgcacc gattttttcc
    30421 atgcgtttag ctaaagcgac actaggaaca acaggaataa cgattatccc agcttcatgg
    30481 aaacgttcca tatacttgct tggatttcct gctcctgttg tgacaacttt aacaccttct
    30541 tcaataacga gatccacgat atcttccaca aagggagata agagcatgat gttgacccca
    30601 aagggtttat cagtcaatga tttgatttta tcaatattgg ccttgacaac ttctttcggg
    30661 gcatttcccc caccgataat tcctaatcct ccagccttgg aaacagcccc tgccaaatca
    30721 ccatcagcaa cccaggccat ccctccttgg aaaataggat aatcaatctt caataattct
    30781 gtaatacgcg ttttcatagt gcctccaacc ttccttgctt acgtaatagt tcgatttcac
    30841 cataatttga cagtcaaact attacctaaa caagagggag tgggtttctc cctactcctt
    30901 ctactaatat tctgcttatt ttgcttgctc ttcaacgtaa gcaaccaagt caccaactgt
    30961 tttcaagtca ttttctgctt cgatttggat atcaaaagca tcttcgattt ctgagattac
    31021 ttggaacaag tccaatgaat ctgcgtccaa atcatcaaaa gttgattcaa gtgttacttc
    31081 tgatgcgtct tttccaagtt cttcaacgat aatttcttgt actttttcaa atactgccat
    31141 gataggactc ctttaaaata aatagttttt ttataacaat gtgttcacca catgattacc
    31201 taaattgtaa gaatgagcgt gccccaggtc aagcctccac cgaagcctga tagaagaaca
    31261 gtctggctac catctaaagg gatgagacct tgttctacac actctgaaag taaaatcggg
    31321 atactggctg cactggtatt gccatattcc atcatattgg ctggaagttt ggctcggtca
    31381 acaccaattt ttctagccat cttatccaaa atacggtcat tggcttgatg aagtagcaga
    31441 taatccaagt ctgtcacctc tataggagat tcatcaatag tctgcttgat agacttggct
    31501 acatctcgaa tggcaaaatc aaagactgtg cgtccatcca tcttcaaaaa tgaatctgca
    31561 ctttcttgat ctgaaaatgg agaatgcaaa cctgaatgcc cataagttaa acactcgctg
    31621 cgacttccat cgctattgag actctcagct aagaaatgct cttgctcgct agcttctaac
    31681 aagacaccac cagcaccatc tccaaacaac acagctgttg atcgatccga ccaatcgact
    31741 gccttagaga gggtttcact accaatcacc aagccttttt gaaagcgacc agaagcgata
    31801 aacttttcag cagttgaaag agcaaataca aatccactgc aagccgcggt taagtcaaaa
    31861 gcaaaggctt tattagcgcc aatattagct tgaacacgag cagctgtaga gggcatcatc
    31921 gaatctggag taatggtagc taggatgata aaatccagtt cttctcctgt tattccagct
    31981 tttgccatca gtttcttagc aacctctgta gccaaatcac tggtagattc tgttcttgaa
    32041 atatgccttt gtcgtattcc cgttcgactt gaaatccact catcattggt atccataatc
    32101 tgagccaagt cgtgatttgt aaccacttgc tctggcacat aatgagcaac ctgacttatt
    32161 tttgcaaaag ccattatttc aaatcctcca aaaattggta aagattagtc aagcctttac
    32221 ccatgacagc aatttcttcc tcgctcatgc catcaataat tttttctacc atggccttgt
    32281 ggaagcgttt atgcagtcta tgaatcaagc gacccttctt tgtcaaatgc agatgcacca
    32341 cacgacgatc ctgttctgac cgaactcgct caatgtagcc cttacgctct aaattgttca
    32401 aacttgtcgt aacagttcca agagttacca tcaactcttt tgacacttga cttggagtca
    32461 catctggagc cttaccgatg acatcaatcg tatgcatttc tttgatggag atatccttaa
    32521 aacgactacc tctcaagttc acttcctcaa ttacaaggac attgttaaat atagatgtta
    32581 aatattcatt gattcgttgg tagtccattt ttcatatccc tccttcttca aaaaactttt
    32641 acaatcaaaa catttgacag aaagaattat atcaaaattc aaagaatgat gcaagtattt
    32701 tttattttcc tgtaaattta ggtcttcttc tctccgaatg agctcgcact ccttctttga
    32761 aatcctctgt ttgagctaag gatttctgta ggttcagttc taaagtagcg taaccctgcc
    32821 agtctttaaa ttggctctcc caaaccaact tcttaatggc agcataggaa ttacttgaag
    32881 cacgtcttaa ttttttaaga agctgttctc tcgtcttttc aagtttttca gcttcagaaa
    32941 cgcggtaaac caacccccat tctagggctt tttctgccgt taaagcctct cctgtcatag
    33001 ctaattgagc agcacgcgtc acaccgatac tgcggctcaa gagatgaatt ccccctgcat
    33061 ctggagccaa accaacacca acaaaggctt ggataaactt agccttatcc gtcgccaaac
    33121 agaaatctgc cgcaacagcc atattcgctg cggcacctgc aacagcaccg tcaacttcca
    33181 ttaaaacagg tttggctatt tgcttgattt tataagaaat cgtattgacc aactctgcga
    33241 tttttgtcaa tgatggaata tcatcctcat ccactgcccg cttcatctct accaaatctc
    33301 ccccaactga gaagaccttt ccattggcat tgattaagat aaaatgcaca gctggatttt
    33361 cttctgccaa agtcagagct tctaaaatct cctcacacat gggaatatga aaaccatttg
    33421 cgacctcagg acggttcaag gtaaggattg ccaagtcctc ttcaagctga taaataatgt
    33481 gttccatcag ggctcctttt tattttataa gcagtttata ctcttcgaaa atctcttcaa
    33541 accgcgtcag cttccatctg caacctcaaa acagtatttt gagctgactt cgtctagctt
    33601 cttagtttgc tctttgattt ttattgagtg ttaaattatt tcactgtcaa agtatatctt
    33661 ttttaggatc agaaagcaag aaaaaatcga ctgaaagtgt ctcagccgat aagaaaggct
    33721 cgctttaggc tagaaagcat cttattttct tcattgaaaa aagcctttct ttctgctata
    33781 atcgtataaa atacttactt taggagttct tatgaaagtt gttaaatttg gaggtagttc
    33841 tcttgcctct gctagtcaat tagaaaaagt tttaaacatc gtcaaaagcg attcagagcg
    33901 tcgttttgta gtcgtttctg cgcctggtaa acgcaatgct gaagatacta aggttacgga
    33961 tgccctgatt aaatactacc gcgactatgt tgcgggtaac gatattagca agagccaaag
    34021 ctggattatc gaccgctatg ctgctatggt tagtgaattg ggactaaaac cagctgtgct
    34081 agaaaaaatt tctaaaagca ttcacgcctt ggccactctt cctattgaag aaaatgaatt
    34141 tctctacgat actttcctag cagccggtga aaataacaat gccaaattga ttgctgccta
    34201 ctttaaccaa aatggtatcg atgcacgcta tatgcaccct agagaagctg ggattgtggt
    34261 cacaagtgaa cctggtcacg ctcgcatcat tccatcaagt tatgacaaga ttgaagaatt
    34321 gacaaacacc aatgaagtcc ttgtcattcc tggtttcttt ggtgtcacta aggaaaatca
    34381 aatctgtact ttctcacgtg gaggatctga tattacaggt tctatcattg ctgctggtgt
    34441 caaagccgac ctctatgaaa actttacgga cgttgatggt atctttgcag cccaccctgg
    34501 tattatccac caaccacact cgattcctga gttgacctac cgtgaaatgc gcgagttggc
    34561 ctatgcaggc ttctcagtcc ttcatgacga ggctcttctt cctgcctacc gtggaaaaat
    34621 tcctctggtt atcaagaata ccaacaaccc tgaccatcca ggtactcgta tcgttctaaa
    34681 acacagtaat gatgaatttc cagttgtggg aattgctggt gactcaggct ttgtcagcat
    34741 taacatgtcg aaatacctca tgaaccgtga ggttggattt ggccgcaagg ttctgcaaat
    34801 cctggaagaa cttaacatcg gttgggaaca tatgccaaca ggtatcgacg atctttctat
    34861 cattctccgt tctcgccaac taactcccat caaggaagaa gaaatcctgc gtcagttggt
    34921 tcaaaaggct aaagtagatc atgcagaaat cgaacacgac ctttctatca ttatgattgt
    34981 tggtgaaaaa atgaagagcc acatcggagt gactgctact gcgacacgcg ctctatctga
    35041 aaacaaaatc aacatccaga tgatgtctca aggttctagt gaagtttcta ttatgtttgt
    35101 tgtcaataaa gaccaagaga aagcagcaat taaagccctc tacaatgcct tttttggaga
    35161 aagtaaggaa gactaagcat ggcccaatca cttaacaaaa cagtactcct cagtacgaca
    35221 ggcacttcct acctctctat cgctgggaaa gttgggaaat tccttgtcgg agatcaggct
    35281 ttggaatttt acccagatgt caatgtcgaa caatttatcc agattccttg gagccatatc
    35341 aatcagattg gagccaatgt cactggacgc aaaatcagcc gccacttcga agtctttaca
    35401 gacaaaggaa aattcctctt tgcttcaaaa gactcaggtg ccattctcaa aattgcacgc
    35461 gaaaaactag gcaatgacaa ggtcgtcaaa cttccgaccc tgattcagac aattagccaa
    35521 aaatttaaaa atctatttgc aaaaaagtag aaactttagt ataatcatag caacggataa
    35581 gtaggttatc ttcttctttc agaaagtctg cgggtactgc gagcagatag gagggatagg
    35641 tgaaattcta ccgttagtaa caattacata cacaatcaag tgcacacctg tgaagttgga
    35701 tggaaccgtg gccctgccac tccaacgttt tgtcaggcgt gcttttttca taaaggagtt
    35761 cttatgttag atatcaaacg tattcgtaca gattttgaag ctgtcgcaga aaaattagct
    35821 acacgtggtg tagatgctgc tgtcttgaat gaaatgaaag aaatcgatgc taaacgtcgt
    35881 aacatcttga tcaaggttga aactctcaaa gcagaacgta acacagtttc tgctgagatt
    35941 gcccaagcta agcgcaacaa ggaaaataca gatgacaaga ttgctgccat gcaaaatcta
    36001 tctgctgaga ttaaagcctt ggatgctgaa ttggcagaaa tcgatgctaa attgacagaa
    36061 tttacaacga ctcttccaaa tatcccagct gacagcgttc ctgttggggc tgacgaagac
    36121 gacaatgtgg aagttcgccg ttggggtact ccacgcgagt ttgacttcga acctaaagct
    36181 cactgggatc tcggtgaaga ccttggtatc cttgactggg aacgcggtgg taaggtaaca
    36241 ggcgctcgct tcctcttcta taaaggcctc ggtgctcgtt tggaacgtgc tatctacaac
    36301 tttatgttgg atgaacatgg aaaagaaggc tatactgaag tcatcacacc ttacatagtc
    36361 aaccatgatt ctatgtttgg tactggtcag tatccaaaat ttaaggaaga tacttttgaa
    36421 ctcagcgata ccaactttgt cttgattcca actgctgaag ttcctctgac aaactactac
    36481 cgtgatgaaa tcttagacgg caaagatctt ccaatctact tcactgctat gagtccgtca
    36541 ttccgttctg aggctggttc tgccggtcgt gatacgcgtg gcttgatccg tttgcaccaa
    36601 ttccacaagg ttgaaatggt caaatttgcc aaaccagaag aatcttacga agaattggaa
    36661 aaaatgacag ccaacgctga aaacattctt caaaaactca accttccata ccgtgtcgtt
    36721 gctctctcta ctggagatat gggcttctca gctacgaaga cttacgactt ggaagtgtgg
    36781 attccagcac aaaacaatta ccgtgaaatc tcaagctgtt caaacacaga agatttccaa
    36841 gcccgtcgtg cccaaatccg ttaccgtgat gaagcagatg gcaaggtgaa actccttcat
    36901 accttgaacg gttctggact tgcagttgga cgtacagtgg ctgcaattct tgaaaattac
    36961 caaaatgaag atggttctgt gaccatccca gaagcacttc gtccatacat gggtggagct
    37021 gaagtcatca aaccataaaa aataaggttt agctatttct agctagacct tttttcgtaa
    37081 ccaaatcaga taagcaccta gtacaaagaa taaaatagtt aggcatataa tggtttcagc
    37141 caataccagg taatccagaa atggaagttt caaaattccc tgagccatct tgagcgaggt
    37201 cgctgtgata atggttggga aggtgagggc tgagaaggct ggttgaaaac cttgttttaa
    37261 aatgttgggc agacgagtta aaacaaagaa aaagaaggat tgagaagcca aaatcatgac
    37321 aatcaagacc caagtcggca ggctggttcc tcctactcga actagagaag ccaagagtag
    37381 agagaaagga gcacagtaga ttccttcttg tccaagcaag gctagtggga gtggatgttt
    37441 ctttaaatcg ctataaataa ggggatagag atagaaggtc aagagaaaac caaaactcaa
    37501 ggtcgcatag gcaatttcga taatacctac cagaggatag gtcaaggcag ccactgctat
    37561 ccccacatag agaaccgtcc aacttggagt ggcatgaacc ctccgccctg gacaagcaaa
    37621 cttgatggta aaaccagcaa tcaaggtcaa atccaagaga aatgaaaacc accaaatccc
    37681 ttgtgctacc aaaggaagat aagagaatac gcgaaagaca taggtcgata aaatcatccc
    37741 agccatagga aaggttgcca ttcctgacaa aagagggggc ttggtcaatt cttgcttggt
    37801 ttctttccaa ttaaagagat gcagaattag aaagtaaatc cataaaacca aaccagtcag
    37861 actaaaaaga tgggatagaa ccggcaacgt atctaaaata agatttccag ctcctgccaa
    37921 acctagcaaa caacctgaaa atactaaggg gagttttttc atcctaacct ccaataatca
    37981 tgttagtttc agtataacat aaaagcgctt aaatgaggat ttaaaaaaac gaagtccgct
    38041 tatttcagac ttcattttac tcagatatga attaggcata aggttgcaat tctggattaa
    38101 ttggtgtatt agctaagttg ttggcatagt tacagaggat tgctaggctg acaccaaaaa
    38161 ccacatccaa ggcattttgt tgagtgtagc cagcttctaa aaactcagac aaggcttcat
    38221 ctcctacacg acccttggta ttgataactg ccaaggtaaa cttagctagg gtatccaatt
    38281 taggatctgt ttcaattgga gtacgattgc gaagagcttg aatcaagtca tcattcatct
    38341 ggatttgttt gatggaaaag gctgtgtgac ctgcgacaca gaaggcacaa ccattggtca
    38401 cggctgccgt gatttgcacc acttcacgct caacgggtgt caggctgttg cgacggtgga
    38461 tagatgagac aatttggtag gcttctaaaa cagtcggggc attggccaag agaccgatta
    38521 ggttgggaat atagccattg ttgtcttttt ctactgtttc aagaatttct ttcacttctg
    38581 ctggtgctga ctctactgta tggatagtaa atgttgtcat aagatacctc ttttcttatt
    38641 attgacacta atattattgg aaaatcttat aaaatcctga ttcctaagtt tatctaagat
    38701 aaagctttat tctctcataa gattttcgtt gttatattag tttatcacac ttccaatcac
    38761 ttgcataata tatattatat atcaggctga taaaaattat ttataggcaa aaaaatcaca
    38821 cgagctgtgt gattccatta tttgtcaaaa tactttttag tttcagcaat aacgactggc
    38881 gacaagacca agagggcaat caagtttggc agagccatca aggcgttaac gatatctgcg
    38941 ataatccaga ccatatccaa ctcgataaat cctcctaaca agaccatgag cacaaaaacc
    39001 acacggtaga gccagataaa gcgaacccca aagaggaact caaaacagcg ttctccgtaa
    39061 tagttccaac ctagaatcgt tgtaaaggca aaaagtacaa ggaagatggt caagagggca
    39121 ggcccaaagt gtgaaaagac tgttgagaaa gctgactgag tcaaggcaac cccattcaag
    39181 tcaccactcc aaactccagt taccaagatg gtcaaaccag ttagagtaca aatgatgagg
    39241 gtatcaataa aggttcctgt catggaaatc aaaccttgct ctactggttc atttgtcttg
    39301 gcagctgcag ctgcaatagg agcagaaccc agaccagatt cgtttgagaa cacaccacgc
    39361 gccacaccat tttgaatagc catccgaacg ctagcaccag caaatccacc taccgcagca
    39421 aggggactaa aagctgaggt aaagactaaa gcgattgtgc cagggatttt tccgatatta
    39481 aagaaaataa ctgtaagagt tcctaagata taaatgatgg ccataaaagg aacaacagta
    39541 gttgaaacct tagaaataga cttgagtcca ccaaagactg caatcgctac aaagacagac
    39601 aagacgagag ctgtgatggc tggcgaaatc gtcgttgtat tttggataga ttctgtaatc
    39661 gagttgactt gggtgaaggt tccgattccc aagagagcaa ccaatactcc tgctactgca
    39721 aacaaaacag caagtggtcg ccacttttct cccatcccta gaaggatata gtgcatggga
    39781 cctcccgcta ctgcaccatg gtcgtccttg gtgcggtatt tgatggccaa gagtccttcc
    39841 gcatacttgg tagccattcc aaagaaagcc gccatccaca tccaaaatag agctcctggt
    39901 ccaccaacct tgatagccgt cgcaactcct atgatatttc ctgttccaac agttgatgcc
    39961 aaggctgtac acagagctgc aaaactggat acatcaccat gtcccttatc ctggataaaa
    40021 ataagctgaa aggccttggg cagacgcaaa acctgcaaga gtcctagccg catggttagg
    40081 taaacccctg ttccgaccaa taaaatcaag aggggcggtc cccaagcaaa atcatcgatt
    40141 gatttaagca attctaacat ttccttctcc tatcgtttca accccaaaag aaagagcaca
    40201 tgcaagatac atgtactctg gaatgcttag ataaatgcta aaaagcggtc tatcctagct
    40261 ctgtcctttt acctgagagt ttgagcagtt gcctgccttg ccccttcggt gcctttacgg
    40321 tctctccaga gttccgtcca tttacagtca tggaaaatca aacgattccc cacttctatt
    40381 aaacttcatt cggtgttggt attaattgat tctaatttta caaaaaatgt tggcttttgt
    40441 caatgtgttt attagtaaaa attagttcaa cagtttttac tttataaagt ccagaatact
    40501 gctatccttt aaaagtgaca atagtcgcac cactgcctcc agcattttgt ggggcatagc
    40561 cgaaactctt gacatgtttg tttctttgca agtatttggt aactccttca cggatgactc
    40621 ctgttccgat accatggatg atatcaactt gagccatatt gttaagcaaa gcttggtcga
    40681 tgaaggtatc tagctcattc atggcttctt catagcgctt gcctcgaaga tccagtctag
    40741 cttgaggtcc tcgcccagaa gttcgtttca caacattgac ctgtttcttc ttgactggtt
    40801 tttcttgctg ggcttgaaca agatcaaact ctttctcttc caaggtcatc ttaatcaagc
    40861 caacttgggc ttcccagcgg ccgtccttga gttgactggt caaggtacca cgctgaccat
    40921 aactgagaac cacgatatca tctcccacct ttggagctcg ttttttctta gccttttgaa
    40981 ggaccttgtt tttagacaag tccacttttt caggagccaa ttttttcaac ttggccttgg
    41041 cttcaatgat ttcgtggggc ttgagttggg atttactgtg gagatttttg agaatctggt
    41101 cactttcact tagggccata tccacaatct cagcagcctg ttcacgcgcc ttgttaagct
    41161 cggtttcctt ttcacgatta agctcgttgt agagtttttt tagcgcacgg ttcatcttga
    41221 gattttcttg ctccacctca cggatattgt ccaaacgttt gcggctttcc agcgtctgct
    41281 cttctaattg ctcaatgata cgattgacgt cattgtcctg atcgatctgc tgactggcat
    41341 ctcctacgat aacttcagat aggcctagac gtttggcaat ttcaaaggca ttacttcggc
    41401 caggaacacc ctgcataaag cgataggtcg ggcgaagagt tgcagtatca aactccatac
    41461 tggcattttg cacaaaggct gtctcaatac cgtaggcctt gagttctgga tagtgggtcg
    41521 tcgccatggt cttgatttga cgcaggcgaa ggtcctccag aatagccatg gcaagggctg
    41581 ctccctcttg gggatcagta ccagccccca actcatccaa aagtaagagt gaatgttggt
    41641 tgaccttgcc aagaatatcc acgatattgg tcatatgact agagaaggta gacaagctct
    41701 gctcaataga ctgctcatct ccaatatcag caaagatttc ttcaaaaata ccaacacgac
    41761 ttcccttgtc tgctaaaatc ggcaatcctg actgggccat gacctgtgtc aagcccagag
    41821 ttttgagcat gatggtcttc ccacctgtat tgggacctgt aatgacaata gctgttaaat
    41881 cttgaccaaa atagacatca tttgcgacgg catttttgac caaaggatgg cagacatgga
    41941 gcagttgaat ctcttgattt tctgacagct gaggcacgac tgcttgtctt tcttggataa
    42001 atcgaacctt ggcacgaatc aagtccagat gaccgataat ccaagcgtca ttagcaatct
    42061 cagccgcatg agggcggaca cgctcagaaa tttcttggag aatgcgaagc atttcatagc
    42121 gctcatctgc tcgcagacta gcaatttctt cgctcagttt gactacctca cggggttcga
    42181 tatagacggt gtttccacta gcagaaatat catgaacgac acctgcaatc ttattgcggt
    42241 aggtgttttt gactggtaaa acctgacggc catttctgct agcaacaatt ccttccgtca
    42301 acagctgcgc tttttgcttg agcaagtctt gtaaaacatc gcgtacctga ctctcgctat
    42361 catgtatttt tcgacggatt cgcgccaatt cttcactggc aaaattttca atgaaacccg
    42421 catcattaaa ggcctgaaga tttccttgta attgcggaaa atcatgtaat ttctcaaacc
    42481 aaagggctaa ttcttccaag ctgacatttt ccagattggt gtaaaaattt tgaagttctc
    42541 ggctggcaag aagcacgcgt ttcaagagta ggaactcctc gatattgaga tccgctccca
    42601 tctccaacct cttgcagact cctgcaattt ccttagttga gagaatagta aaatgcggtt
    42661 gctcgacgaa aagagcctgc atttccttca tctcagcaaa agcctgtttg attttatctg
    42721 ctttggcagt cggagccagt tgtctcaatt gctccaagcc ctgctcggtc aacaaatgag
    42781 gctcaaacaa ggccttgacc ttatcgaact ctaatgtttc taatattttc ttattcatct
    42841 ttatttcctt gtctttgaat gtctaggtgt ggtagctttt tacattctga tagattcgag
    42901 tcaaatctgt aaacaaaggg ctaggaaaac tcctgccctt tttatccgat taaatttgtc
    42961 acccagattt gtttgagcca actggttgtt accggtatgc tctggatgat gtgttttgcg
    43021 acgatactct tttcaagagg attttgtata actgccatgg ggatggtcgc caagattgtc
    43081 aaggccattt gcaagacaaa taaggtcacc aacatggaca agatacctgc tgaaacttgg
    43141 aacaacttac cacccagttt tttgctagga atcaagtgta agagaagacc aagtaaacga
    43201 ccaatgctat agacaatccc aaatacaagc aagtagccga tacctgcata aaagacctta
    43261 tccagctgaa agagttgatc cgatgggaaa aagaaagtcc cctgaccttc ctgcgaattt
    43321 gcataaggga gcaataaatg gaattgctct ccaagcccct tataaaactg gccagccata
    43381 aaagccgatg ccatggctga aatcaggtaa taaacctgta agagcaggcc tctccgatag
    43441 ccgatataaa atccccaaac caagaccaat agaagaagga atgaaatcat aaggaatcct
    43501 caatcttgct ctgttcttgc ttacaagtca caagcttgtg acggagttct tctagctctt
    43561 gctccttatc gtcaaattca atctcacggc tgagctgagt tgataaacag ttgactgcca
    43621 acaaaagagc gattgtttca tcatctgcgc taggcatttg ttctttaatt gcttggtatt
    43681 tttctgtcgc aaccttagcg atttcctcca taaaaaggtt gtcatgttcg cttgtcaagg
    43741 ttaacgattt tttcccgaat gtaaatttga atcgatttag atttgccata aaattcacct
    43801 cacgatatta taccaaaatt cgctaatttt gtcagttttt acaaatttta ctgcttttgt
    43861 ggtacaatag aaactatggc aagtatcaca ctcacaccaa gcgaaaagga tattcaggct
    43921 tttcttgaac actatcaaac cagtctggct cctagcaaga atccctatat ccgctacttt
    43981 ttgaaactac ctcaagcaac ggtttctatc tatacttctg gaaaaatctt gcttcagggt
    44041 gaaggggctg aaaaatacgc cagtttcttt ggctatcaag ctgtagagca aaccagcgga
    44101 caaaatcttc ctttaattgg gacagatgag gtgggaaatg gttcctactt tggtgggctt
    44161 gcagttgtgg ctgcctttgt cacacctgac cagcacgact ttttacgaaa actcggtgtg
    44221 ggggattcta agactctgac cgaccaaaag atccgtcaga ttactcctat tctcaaggaa
    44281 aaaattcagc accaggcact ccttctctca cccagcaagt acaacgaggt catcggagac
    44341 cgctacaacg ctgtttcggt taaggttgcc ctccataatc aggctatcta tctcctcctt
    44401 caaaaaggtg ttcagcctga gaaaattgtg attgatgcct ttaccagtgc taaaaattat
    44461 gacaagtact tggcacaaga ggccaatcgt ttcagcaatc ctatcagctt agaagaaaag
    44521 gctgagggca aatacttgtc tgtcgcagtt tcttctgtca ttgcgcgtga tctctttctg
    44581 gaaaatcttg aaaatttggg acgagaactg ggttatcagc tcccaagtgg agctggaacg
    44641 gcttctgaca aggtggctag ccagattttg caagcctatg gtatgcaggg actcagcttc
    44701 tgcgccaaat tgcactttaa aaacactgaa aaagcgaaaa aacgcttaga aaggtaagtt
    44761 atgaattcat ttaaaaattt cttaaaagag tggggattgt tcctcctaat tctgtcatta
    44821 ctagctttaa gtcgtatctt tttttggagc aatgttcgcg tagaaggaca ttccatggat
    44881 ccgaccctag cggatggcga aattctcttc gttgtaaaac accttcctat tgaccgtttt
    44941 gatatcgtgg tggcccatga ggaagatggc aataaggaca tcgtcaagcg cgtgattgga
    45001 atgcctggcg acaccattcg ttacgaaaat gataaactct acatcaatga caaagaaacg
    45061 gacgagcctt atctagcaga ctatatcaaa cgcttcaagg atgacaaact ccaaagcact
    45121 tactcaggca agggctttga aggaaataaa ggaactttct ttagaagtat cgctcaaaaa
    45181 gctcaagcct tcacagttga tgtcaactac aacaccaact ttagctttac tgttccagaa
    45241 ggagaatacc ttctcctcgg agatgaccgc ttggtttcga gcgacagccg ccacgtaggt
    45301 accttcaaag caaaagatat cacaggggaa gctaaattcc gcttatggcc aatcacccgt
    45361 atcggaacat tttaagaaac ctaagaggca gagaatcacc aatctcagcc tcttcttcta
    45421 tcgtgagaag tgattgttta tttacaaata ctcaatgaaa atcaaagaac aaactaggaa
    45481 gctagccgta ggctgcttaa aacagtgttt tgaggttgtg gatagaactg acgaagtcag
    45541 ctcaaaatac tgttttgagg ttgcagatga aagctgacgt ggtttgaaga tattttcgaa
    45601 gagtataaaa taaaaacaat cccaactctc acctattcat gcaaaggaat tttatggaag
    45661 tttatttttc aggaactatt gaacggatta tttttgaaaa tcccagcaat ttttatcgca
    45721 tcctcctcct agaaatcgac gatacggacg cagaggattt tgatgatttt gaaatcattg
    45781 tcacaggaac catggctgat gtaattgagg gcgaagacta tactttttgg gggcaaattg
    45841 tccagcactc caagtatgga gaacaactgc aaatcagtcg ttatgatcgc gcaaaaccaa
    45901 ctagtaaggg cttggtcaag tacttttcaa gtagccattt caaggggatt ggtctcaaga
    45961 cagctcagaa aatcgtggat acctatggcg aaaataccat tgacgaaatt ttgcaacacc
    46021 cagaaaagtt agaaggcatc gcaggactct ctgccaaaaa tcgcgaggct ttcgtctcca
    46081 ctctccgtct caactacgga acggagatga ttttggccaa actagccaac tacggcattc
    46141 ccaacaaact agcctttcag attcaagact tttacaagga agaaaccctt gatgtggttg
    46201 aaaattatcc ctaccagctg gttgaggata tcaagggttt gggctttacc attgctgacc
    46261 aactagctga ggaactaggc atcgaaagtc aggctcctga acgcttccgc gccggtctag
    46321 ttcacagtct ttttcaggcc tgtatggaaa caggggacac ctatgttgaa gcacgggatt
    46381 tgctggaaca aacccttact ctccttgagt cttcccgtcc cgtggaacta gaccccagcc
    46441 aagttgccca agagctctcc tacctgatcg aagaagacaa ggttcagcag attgatacca
    46501 agatctttga caacagcctc tttttcgctg aggaaggcat ccgcagtcac ttggttcgta
    46561 tccttgaaaa aggaaaacag aagagtcatg atttagaaac cattcaaaaa catatcacta
    46621 ctgtcgagca agaactggga attgagtatg ataacattca aaaacaggct atctgtgatg
    46681 ctatccagaa taaggtcttt atcctgacag gtgggcctgg tactggtaag acaactgtta
    46741 tcaatggaat cattgctgtt tatgcccttt tagaaggact tgacttcagg aagaaaagca
    46801 atctgcccat tcttcttgct gctccaactg gtcgagctgc tcgtcgcatg aatgaattga
    46861 caggtttgcc tagcgcgacc atacatcgcc acttgggaat gacaggtgac gatgatacca
    46921 gtcatctgga agattaccta gatgctgact ttatcatcgt ggatgaattc tctatggtag
    46981 atacttggct ggccaatcaa ctcttctcca atatctcttc taacagtaag atcctcatcg
    47041 taggtgacag tgatcagcta ccttctgtca gtcctggaca ggttctagcg gatctacttc
    47101 atattccttt gattcctcag actcgcttgg aaaaaattta tcgacaaagc aaagaatcaa
    47161 ccatcgtcac cctagctagt cagattcgac agggcatctt gccagctgat ttcacccaaa
    47221 aaaaagctga ccgttcctac tttgaaattg ctagtggcca tattcctgcc accattgaaa
    47281 aaatcttagg tgccgccctc agaaatggta ttcccgcccg tgatatccaa gttctggctc
    47341 ctatgtaccg agggacggca gggattgatg ctatcaatca gctcatgcaa gacctgctca
    47401 atcccccaca aaaagatcaa ctcagttttg aagctcctca gtgtcactat cgtaagagag
    47461 acagggtcat tcatttggtt aacgatgctg aaatcaatgt ctttaatggg gatttaggag
    47521 ctatcacaga cctgattcct ggtaaataca ccgaatcgaa acaagacgag attgtcattg
    47581 attttgatgg taatgaagtc tcttaccccc gtaacgaatg gtacaagatt cgcttggcct
    47641 atgccatgag tattcataag tctcagggaa gtgagttccc agttgtcatc ctaccgatta
    47701 ctagtgctag ccgacgtatg ctggagcgaa atctcatcta cacagccatt acacgtgcca
    47761 aaagcaaact catcttacta ggcgaattac aagccttcga ctatgctacc caacacatcg
    47821 gaactgcccg aaaaacctat ctgattgaac gcttcagtga tctactggag aatgttgaag
    47881 aaaagcaaca agctgtctct gaaacagtca catcaagtgc ctctgaacaa tcctacatcc
    47941 taaccgaaga aaactgggac cgcatcccag ccatgattgg gattacagac acagacctca
    48001 aagagatttt tggaaaatag tgcatcagaa aacccaccaa ttcaggtggg ttttctgttt
    48061 attaagatta ttttactgtt gctgtgcttg tgatcaattc aacagctttt ttgatagtga
    48121 tatcatgttt caacatgtca gctgaaagca agttttgaac ttgtgcaact tccatgttgt
    48181 agtctgctgc caattgctca acttcttttt ggatttcttc ttctgaagca tcaaatcctt
    48241 cagctttggc aactgcttcg ataacaaggt tagtcttagt acgtgactca gcttctgctt
    48301 ggtattggtt gtgaaggtct tcttgagtag ttccagtgat ttggaagtac atgtcagggt
    48361 tgatcccttg acgttgcaaa ttcccaagga attcatttac tgaacggtga acttcttcat
    48421 ggatcatttc ttctggaagt tctacgattt cagcattttc tacagctgta tcaattgctg
    48481 caccttcaac tgcatctttg taagcttctt ctttagcagc agccaattct ttgctgtatt
    48541 tttctttcaa gtcagcaagt gtttcaactt cttcatcaat gtcttttgca agttcatcgt
    48601 caagagccgg aacttcttta gcttttactt cgtggatagt tgtcacgaat ttagcttctt
    48661 tacctgcaag gtcttctgct tggtagtctt ctgggaatgt tacgataaca tcaacggttt
    48721 cgccagctga gtgacctacc aattggtctt cgaaaccagg gatgaattga cctgaaccaa
    48781 gtccaagtga gaagttttca ccttttccac cgtcaaattc aacaccgtcg atagaaccaa
    48841 cgaagtcgat cacaacagtg tcgccgtttt cagcagcagc ttccttgata accaattcag
    48901 ccaggttgtt gcgttcgcgt tcgatacgct cttcgacatc agcgtcagtt acttcttttt
    48961 ctacatcaac tgatacttca aggtttttgt agtcacccaa ttttacttca ggttttgtaa
    49021 cgacttcagc agtgataacc cagtcttgac ctttttccat tgaagttacg tcaatttttg
    49081 gttgggcaac cacttcaaga ccagcttctt ttacagctgc ttcataagcg tttggcaaaa
    49141 gtgcgttcat tgcatcttga taaagagctt cttcaccaaa tttttggtcg aagatagggc
    49201 gtggaaggtg acctttacgg aaacctggaa cattaagaga tttcttcact gacttgaaga
    49261 cacggtccaa ttctggtttg atttggtctt gagagatagt gaaagtcaag acaccacggt
    49321 ttgtttcttt gttttcaaat gatacagaca ttctgtcatt tctccttaaa attttttaaa
    49381 tacagtctat tataacattt tctataaata aaatctagac taatgcagac aattatagac
    49441 aacaagcctt tttcctaact aaaacactca taaagtatag acaattttag actactttgg
    49501 atatcttttg gttagtcatt ttccaaaact aatttatcta atttttcagt tatatagtag
    49561 attgaaacta gaatagtaca cctctacttc taaaacattt ttagaaatcg atttgactgt
    49621 cctgatcgat ttgtcctgtt cttgtttcat tttactatat agatcgcatt cgaaagaatt
    49681 cgttccaaaa ttgcttggtt cgttcttctc ttggacgact aaagaattga tgggcattat
    49741 ttttttcaac aattttccca ctgtccataa aaataacatg atgggctaca tctctagcaa
    49801 gacctatctc ataaagctga tcaaaaactc aatctgagga gaaaatccaa taaggctttc
    49861 cataataaag gacatcctat caagattttt gaacacccga taggctgcct tttcccgatt
    49921 ttgaagactt tttgtccagc ttcttttacc ttataaataa caacaaaaat caaagacaag
    49981 ctagaaagat taccgcagat aactcaaaca gtgttttgag attgcagata agactgacgg
    50041 agtcagctca aagcactgct ttgaggttgt agatagaaca acatatgcct aagacaaagc
    50101 gatgctaacc tgatttgaag agattttcga aaaagattaa atatactcta caatttgctc
    50161 aatcaattct tggtcgtcta aacctgtaac ttgtataaca acatctttga ctgattgttt
    50221 agccaacaat tcacctaatc gaatactttc ttcatcattt acatcgcgat agtcaaagac
    50281 atagccaact gttttaagta ggtttttata tgacaaactg agttctttca attcacgtat
    50341 cggccggatg aatcgttcat tatagcctaa ttttcggatt ggagtacgag ctacgcgact
    50401 aacctcgtcc actatgaaag ggttttcaag ttgttctata atgactttgt gataattctc
    50461 caattctttt ttatcaaagt tccatttggc aatcaagaga ctccgaattt cagctaatac
    50521 agattcaatc cgagatttaa tattaggatt ttgaagagct tccaaaattg tcttggcacc
    50581 ataatgcgca ccaatgtaag ctgaagttgc atgtccagaa ttgactgaaa aaagttttcg
    50641 ctcaataaag ggttctaaat cttcttcata atgcacatct tttagacgta aatctggatt
    50701 tttaagacgc ttggtttcca cgacccattc attaaagggc tcgaccacaa caaaaaggga
    50761 atcttcgtga ctttgtgctg gaacaatcct gtctactgca gcatttggaa aacctatgta
    50821 gttatcagca aatgtcaaac cttccggact taaatatttc ttgacttctt gataaagaaa
    50881 ttgagacccg ccaatcatat tttcacaggc cataacatcc aatgcctgtg tatttcctgc
    50941 aactcggcga gcttcgattc ctttggctag aagttcggcg ataaaaggga gtatattagg
    51001 tccgattgca gtagtaataa tatccgtctt ttgaatcgct tcaatgactt gctcaggatg
    51061 ttctttgcta ttaatgccag ccacattagt tacttctata cgagactgtc ctttctgtgc
    51121 aatttcaatt tcatacttgc ccttttcatt cagagcatga attatctgat tattgacatc
    51181 cacaaaatca atatggaaac catttttaaa tagaatttca cctataaaac cacgaccgat
    51241 attaccggca ccaaaatgaa cagaatgttt cattcaattt ctccttttcc tatttgaggg
    51301 acatatatac tagatgacaa atgactaaat tcaattattc aacagcatca aataagcgca
    51361 atacttcctc ttttgactga gcatctgcta gtttaagaac attatctaca tctgcacaga
    51421 agatagaaat tttctgaata atttctaagt gttcattacc aataccagca ataccaaaaa
    51481 gaaccgttgc cacttgaggg ttagatacat tcccaaaatc aacccctcta gggacttgaa
    51541 cgactgtaat accagacttg aggacatcat tttttgctgc atctgttcca tgcggtatgg
    51601 cgataaagtt acccatgtaa acagatagct ctttatctcg ctcaatcatg gcttcaatat
    51661 agtcttcatt aacatatcca ccctcataaa gaacttgccc acaataacaa atagcttcct
    51721 ccttgttaga aaattgttta ttaagcttaa tcaaatgttt ttcaagtttc atattagttc
    51781 tccaatttct taattttttc gttaaaaata gtatttagca tctgataaat aatggattga
    51841 tttcccgtct tgtaaatctc tgtataaaga tgattttcaa taatcgactg actaatagct
    51901 gtcattaaat ctctagcttc ctcgctagct tctttagaca ttagcattag cagacacctt
    51961 ttgacctctt cttcctctcg tttcattgac aatgcggaga taggtagttt gagttcaaac
    52021 atagtaaaac tatttgttgt tactttgcta gactgactat gtaaaagcac cagaccagta
    52081 ttaggaatag ccataggact attctggaag tgagataaca attttcttgt taggtaagct
    52141 ctgtcactca cattcttctg atattggatg atttgttcta ccgtgtgctc aaatgattga
    52201 ttattctcca attgaaccaa ctcgaattga ctcaaaagct gactactaga tattaaataa
    52261 tcttgcaagt cataactttt ctctggagcg atagcatcac gcgcgacaat tgtacgatgt
    52321 gcctgtacat actgaagtcg ttcctgtaat tcaagcaatt ctttgacggt tggaaaacta
    52381 gaaattacat cgatttcctg attagtcagc ggcttggtag ataaaatata atcatactga
    52441 ctgagatcaa tactgtggta atctactaga gattgaatat ctatcaactc tacaaaagga
    52501 gcaacactct taattttact gactaataaa tctctagtga cccgacgttc atccgttaaa
    52561 agcaaaacac ggactggata aatctctgga ctacggccta agctagagat aaaatgtagg
    52621 gcaatcatgc catactcata ctctgtatga agatatttcg gaaaaatatc attcactaaa
    52681 agactgatga ctgtataaag aaatttattc ctttcaacta aaatctggat agattctggc
    52741 aaattttcac cctgaaaaag gatagggacg cctaaactga gccgaatatg atggaaaaga
    52801 aaattgaata aaaccttgtc cttaaaaaag tcaatcttgg tatacatgga aaccgtatca
    52861 atcagatttg aaatattgta gaaaaattca ccatcaaatt tctccgtaaa gagcggattg
    52921 tcctgacgtt taatgattaa ttcatccaag atgctcgcaa aatagataat ttcctgaata
    52981 ctataaaatt gtgcagtctg cttagagtaa gcttgaaaaa ttttttgaga aatttccaaa
    53041 gcctgcttac tagtattagg tgaattttca atgttttgct cctgacctat aagagataac
    53101 aagatcgcaa aaaacatctt catcctagca tccatatctg gaaaacctga cagttgctta
    53161 ttaacaatct gactggccag cccagttcta tctgcttcta aaatatcaaa gctcccaaaa
    53221 ttaccggttg aaaaatctgc tactgagata cagtttgtca gtaaaatagc caaaaatctt
    53281 ctcttaccaa ctgaatcacc agaaatccga taaccttttt gtcgttcaat tttcagatca
    53341 aagtctaaaa gacgcttatc aatatctgaa atatcctgaa taatagttac attactgact
    53401 ttcgtgcact cttgcaatgc ttcattggtg acaaaaccac tctcagtcag aaggcgatag
    53461 gtaatcaact cttgtctttc ttggggacta tactcaacta acacttcaag ttctgtcggc
    53521 aaatcatcca actctccagt caaaatatag tatttcccat gcttcgttat ttcgattcca
    53581 tattgctcca tgctatctgt caaatctgat aaagttcgat aaattgttct agatgaaacc
    53641 tgtaagattt cagtcatatc ttgcattgaa agcttcccta catgtaggaa agccttcaat
    53701 aattgttctt ctcgtttggt taatagcatt cgtaatttag aaacctctag ttctatttgt
    53761 acattctagc agccattttg tcatattctg gtgttgttac taaactatcc acaataagaa
    53821 attgagaatt cggtgctgct tgctgcactt ctgcctgtaa agaaatagtt gttacaatca
    53881 ttatgttttt agaattaaat tcacctaatt ctgaaatttt ggcagtagaa actggaatac
    53941 gaatattctt gtttctaaaa atagcccgaa tcgtttcaca gcccatagtt gcagttccct
    54001 gtgctttacc ataagcaact actacagcat caatatggtt aaggtcactt tcctgactat
    54061 ctactggtgc tgaagttggt atatctcctt caatttctgc tattggagaa gctcctgtta
    54121 atgaagctac aatttcatca taacgagagg acgctaagaa attatcaaca gaaacatgaa
    54181 tagcacttgg actcttgtct ttagctcttg gtgtcagttc ttcctgagta acaattaatg
    54241 tttttggtgt atcaagcaaa tttgagattg cctgattaga tactggaatc tctagacctg
    54301 cttttttaac cttatctcga agaatactag ctcccatagc agagcttccc ataccagcat
    54361 cgcaggcgaa aatgattttt tccactgagt ctgtcgaaac aactgcatca acagaagttg
    54421 atactaactg acctttagac tgagccttag ctgcttgggt agccgcctga gcagcttcga
    54481 gcgaatcctc agttgacttg tctgcatgaa gaataagggc tgctacaagg aaagaaacaa
    54541 ctgctgccac taaaacacct aaaagaacat ttagatgggg ccaaacacct tttggcgccg
    54601 tagctataat cgcaataata gaacctggtg aagctggaga tttaagacca gcgtctaaga
    54661 gttgaaaagt aaaagttcca gagatacctc ctgccatagc agctaaaaat agagtaggct
    54721 tcatcataac ataaggaaag taaatttcat gaatccctcc gaagaaatga ataaccattg
    54781 ccccccaaga agaagattta gcagaacctt taccgaatac agcataagct aatagaattc
    54841 ccagacctgg tccaggatta gcttccaata ggaagagaat tgacttacca gcttgagcta
    54901 cctgttctac tcccagagga gtaaaaatgc catgattgag ggcattattg aggaaaagga
    54961 ctttagccgg ttcgatgata atattagcca taggaaggag gcgagcattg acaatagcct
    55021 caacaccatt cccaacagct ccagtaagag tcgatacgac tggaccgatt gcgtagaaag
    55081 ccaaaagcaa taatgcaaaa ccaacgagac cagctgagaa gttattaact aacatttcaa
    55141 atccgggacg aattttttcc tggaacttct catcaaattt cttgatagtc catcctccca
    55201 gtgggcccat taccatagct ccgataaaca taggaacact agaacctgtg attgcaccaa
    55261 cagtagcaat agctcctaca acggcaccac gttggccatg gatcatatat ccacctgtgt
    55321 aaccaatcag gattggcaat aaatacgtta acataggacc aacaacagta gctaactgtt
    55381 catttggcag atagccatca gcgataaaaa gggcagtcaa tactccccaa gcaataaatg
    55441 ctccaatatt gggcataacc atatttgaaa gcgatgtccc tagtttttga accctgactt
    55501 tcaatgatac tttttcttcc attagatcat cctccttttc ttaaaactag tatatacaaa
    55561 gttaaaaacc tttaaaacta aagttttgtc acagattttg tgtcaaaaca ttacttttga
    55621 cgaggctggg caaaaaggct t
//
LOCUS       DAVDWM010000009        55220 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVDWM010000009 DAVDWM010000000
VERSION     DAVDWM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.009"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            337..855
                     /locus_tag="VUW49_000662"
     CDS             337..855
                     /locus_tag="VUW49_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET7980619.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            985..1491
                     /locus_tag="VUW49_000663"
     CDS             985..1491
                     /locus_tag="VUW49_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7980620.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            1491..1661
                     /locus_tag="VUW49_000664"
     CDS             1491..1661
                     /locus_tag="VUW49_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980621.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            1693..2925
                     /gene="clpX"
                     /locus_tag="VUW49_000665"
     CDS             1693..2925
                     /gene="clpX"
                     /locus_tag="VUW49_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7980622.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            2934..3521
                     /locus_tag="VUW49_000666"
     CDS             2934..3521
                     /locus_tag="VUW49_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET7980623.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            3532..3912
                     /locus_tag="VUW49_000667"
     CDS             3532..3912
                     /locus_tag="VUW49_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7980624.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            3964..4854
                     /gene="rapZ"
                     /locus_tag="VUW49_000668"
     CDS             3964..4854
                     /gene="rapZ"
                     /locus_tag="VUW49_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET7980625.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            4851..5828
                     /locus_tag="VUW49_000669"
     CDS             4851..5828
                     /locus_tag="VUW49_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET7980626.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSNHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            5825..6736
                     /gene="whiA"
                     /locus_tag="VUW49_000670"
     CDS             5825..6736
                     /gene="whiA"
                     /locus_tag="VUW49_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET7980627.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETDIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKIINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(6770..7738)
                     /locus_tag="VUW49_000671"
     CDS             complement(6770..7738)
                     /locus_tag="VUW49_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7980628.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            7874..8731
                     /locus_tag="VUW49_000672"
     CDS             7874..8731
                     /locus_tag="VUW49_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET7980629.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            8718..9497
                     /locus_tag="VUW49_000673"
     CDS             8718..9497
                     /locus_tag="VUW49_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET7980630.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            9521..10873
                     /locus_tag="VUW49_000674"
     CDS             9521..10873
                     /locus_tag="VUW49_000674"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7980631.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            10947..11321
                     /locus_tag="VUW49_000675"
     CDS             10947..11321
                     /locus_tag="VUW49_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET7980632.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            11323..12171
                     /locus_tag="VUW49_000676"
     CDS             11323..12171
                     /locus_tag="VUW49_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7980633.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            12336..13103
                     /locus_tag="VUW49_000677"
     CDS             12336..13103
                     /locus_tag="VUW49_000677"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7980634.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            13115..14299
                     /locus_tag="VUW49_000678"
     CDS             13115..14299
                     /locus_tag="VUW49_000678"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET7980635.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFIFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            14296..16167
                     /locus_tag="VUW49_000679"
     CDS             14296..16167
                     /locus_tag="VUW49_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7980636.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(16310..17494)
                     /gene="mntE"
                     /locus_tag="VUW49_000680"
     CDS             complement(16310..17494)
                     /gene="mntE"
                     /locus_tag="VUW49_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET7980637.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            17798..20494
                     /locus_tag="VUW49_000681"
     CDS             17798..20494
                     /locus_tag="VUW49_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7980638.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVDLATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            20507..21298
                     /gene="yghU"
                     /locus_tag="VUW49_000682"
     CDS             20507..21298
                     /gene="yghU"
                     /locus_tag="VUW49_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET7980639.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYTPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            21414..21824
                     /locus_tag="VUW49_000683"
     CDS             21414..21824
                     /locus_tag="VUW49_000683"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7980640.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            21836..23431
                     /locus_tag="VUW49_000684"
     CDS             21836..23431
                     /locus_tag="VUW49_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980641.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            23498..23662
                     /locus_tag="VUW49_000685"
     CDS             23498..23662
                     /locus_tag="VUW49_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET7980642.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            23741..24469
                     /gene="yaaA"
                     /locus_tag="VUW49_000686"
     CDS             23741..24469
                     /gene="yaaA"
                     /locus_tag="VUW49_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET7980643.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(24746..25294)
                     /locus_tag="VUW49_000687"
     CDS             complement(24746..25294)
                     /locus_tag="VUW49_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7980644.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGASALQDKFVTISSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            25615..26103
                     /locus_tag="VUW49_000688"
     CDS             25615..26103
                     /locus_tag="VUW49_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET7980645.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            26100..27287
                     /locus_tag="VUW49_000689"
     CDS             26100..27287
                     /locus_tag="VUW49_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7980646.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVIVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     CTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            27280..27504
                     /locus_tag="VUW49_000690"
     CDS             27280..27504
                     /locus_tag="VUW49_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET7980647.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            27661..29007
                     /gene="asnS"
                     /locus_tag="VUW49_000691"
     CDS             27661..29007
                     /gene="asnS"
                     /locus_tag="VUW49_000691"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET7980648.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            29160..29450
                     /locus_tag="VUW49_000692"
     CDS             29160..29450
                     /locus_tag="VUW49_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7980649.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            29462..29932
                     /gene="ssbA"
                     /locus_tag="VUW49_000693"
     CDS             29462..29932
                     /gene="ssbA"
                     /locus_tag="VUW49_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET7980650.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            29964..30203
                     /locus_tag="VUW49_000694"
     CDS             29964..30203
                     /locus_tag="VUW49_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7980651.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            30498..31898
                     /locus_tag="VUW49_000695"
     CDS             30498..31898
                     /locus_tag="VUW49_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET7980652.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            31900..32259
                     /locus_tag="VUW49_000696"
     CDS             31900..32259
                     /locus_tag="VUW49_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET7980653.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHYLKKKK"
     gene            32313..33062
                     /locus_tag="VUW49_000697"
     CDS             32313..33062
                     /locus_tag="VUW49_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET7980654.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            33052..33351
                     /locus_tag="VUW49_000698"
     CDS             33052..33351
                     /locus_tag="VUW49_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET7980655.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            33422..34357
                     /locus_tag="VUW49_000699"
     CDS             33422..34357
                     /locus_tag="VUW49_000699"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET7980656.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNIRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            34444..34956
                     /locus_tag="VUW49_000700"
     CDS             34444..34956
                     /locus_tag="VUW49_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7980657.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYLEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            34993..35511
                     /locus_tag="VUW49_000701"
     CDS             34993..35511
                     /locus_tag="VUW49_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HET7980658.1"
                     /translation="MIRYLDQYEDVILCENKRYYLNFPMLESLDSLELDQEIFVREAS
                     PVYQALLEQSFETELRNQINAAILVEKTDFARTKMTLSNYFYKVKQQYPLTEKQQELY
                     DILGDVNPEYALKYMTAFLLKFLKKDQLMQKCRDIFVDSLVVLGYIVQNEDGKYELAI
                     DFDKERLTFYLA"
     gene            35612..35794
                     /locus_tag="VUW49_000702"
     CDS             35612..35794
                     /locus_tag="VUW49_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7980659.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(35840..37285)
                     /locus_tag="VUW49_000703"
     CDS             complement(35840..37285)
                     /locus_tag="VUW49_000703"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET7980660.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLREQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QQPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            37391..39013
                     /locus_tag="VUW49_000704"
     CDS             37391..39013
                     /locus_tag="VUW49_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET7980661.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            39216..41174
                     /locus_tag="VUW49_000705"
     CDS             39216..41174
                     /locus_tag="VUW49_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7980662.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            41346..43028
                     /locus_tag="VUW49_000706"
     CDS             41346..43028
                     /locus_tag="VUW49_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980663.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTIYSSSKGSSVALENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENR
                     PVLKDITCSIFKGQKIAFVGPSGSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKE
                     WRSKIAWVSQNNAVLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDT
                     EVGERGRLLSGGRSQRLQIARAYLKDAEILIFDEATANLDADSEYAIISSLYSVLKEK
                     TVVIIAHRLSTVKDVDCIFFLEEGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            43186..44280
                     /locus_tag="VUW49_000707"
     CDS             43186..44280
                     /locus_tag="VUW49_000707"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET7980664.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            44288..45454
                     /locus_tag="VUW49_000708"
     CDS             44288..45454
                     /locus_tag="VUW49_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7980665.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            45547..48645
                     /locus_tag="VUW49_000709"
     CDS             45547..48645
                     /locus_tag="VUW49_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7980666.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQEFLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGVVLFHKGQVYFLTDQQIRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDVDLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVTVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLLQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPSKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            48693..49310
                     /locus_tag="VUW49_000710"
     CDS             48693..49310
                     /locus_tag="VUW49_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET7980667.1"
                     /translation="MKQERFPLVSDDEVILTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            49321..50655
                     /locus_tag="VUW49_000711"
     CDS             49321..50655
                     /locus_tag="VUW49_000711"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7980668.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            50665..51129
                     /locus_tag="VUW49_000712"
     CDS             50665..51129
                     /locus_tag="VUW49_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980669.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDYQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            51126..51620
                     /locus_tag="VUW49_000713"
     CDS             51126..51620
                     /locus_tag="VUW49_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980670.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            51701..53356
                     /gene="mltG"
                     /locus_tag="VUW49_000714"
     CDS             51701..53356
                     /gene="mltG"
                     /locus_tag="VUW49_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7980671.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            53427..53909
                     /gene="greA"
                     /locus_tag="VUW49_000715"
     CDS             53427..53909
                     /gene="greA"
                     /locus_tag="VUW49_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7980672.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            54282..54680
                     /locus_tag="VUW49_000716"
     CDS             54282..54680
                     /locus_tag="VUW49_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980673.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            55125..>55220
                     /locus_tag="VUW49_000717"
     CDS             55125..>55220
                     /locus_tag="VUW49_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7980674.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNRE"
BASE COUNT        16326 a        10403 c        12583 g        15908 t
ORIGIN      
        1 tcatttacct ttcctaatcg acttggtttc atcttatttc aatctattat agtattgggg
       61 aatttcttca aaccacatca gcttggtcag ttctacctgc gacctcaaaa cttgtgcttt
      121 ggtcaagctg ggtttagttt cctagtttgc tgatggattt ccattgacta taagcatcca
      181 accctctttt tgtcttctaa agaattctta aattatcagt ctattgcaac ttttctcata
      241 taagttcttt gtcttgctat tggttttcct tagtagtata ctaaggtagt aatcattaag
      301 aagtggttac aaaaaataat gaatgaggta aagaaaatgg tagaattgaa aaaagaagca
      361 gtaaaagacg taacatcatt gacaaaagca gcgccagtag cattggcaaa aacaaaggaa
      421 gtcttgaacc aagctgttgc tgatttgtat gtagctcacg ttgctttgca ccaagtgcac
      481 tggtatatgc atggtcgtgg tttccttgta tggcatccaa aaatggatga gtacatggaa
      541 gctcttgacg gtcaattgga tgaaatcagt gaacgcttga ttacactcgg tggaagccca
      601 ttctctacat tgacagagtt ccttcaaaat agtgaaatcg aagaagaagc tggtgaatac
      661 cgtaatgttg aagaaagctt ggaacgtgtt cttgttatct accgttactt gtcagaactt
      721 ttccaaaaag gtttggatgt cactgatgaa gaaggtgacg atgtgacaaa cggtatcttt
      781 gcaggcgcta aaactgaaac agataaaaca atttggatgc ttgcagccga acttggacaa
      841 gcacctggtt tgtaagaaat aaggaaacat agataaatct gtaggaaatt tcttacagat
      901 tttttctatt ttgtaagcta ttccaaacca gtcttttttt gagttttttg ataaaatagt
      961 actatcagtg aaaaggatgg aagcatgact aagaaaatcg tagctatttg ggcccaggat
     1021 gaagagggtg tgattggtaa ggaaaatcgt ctgccttggc atttgccagc agaattgcag
     1081 cactttaaag aaacaactct gaatcatgct atcttgatgg ggcgtgtgac ctttgatggg
     1141 atggggcgtc gcttgcttcc aaaacgggaa accttgattt tgacgcgtaa tccggaagaa
     1201 aagatagatg gggttgctac ttttcaggac gtccagtctg ttcttgactg gtatcaggct
     1261 caagaaaaga atctctacat tatcggtggg aagcaaattt ttcaggcttt tgaaccttac
     1321 cttgatgaag tgattgtcac tcacattcat gctcgggtgg aaggagatac ctatttccct
     1381 gaagaacttg acttgtctct ttttgagaca gtttcaagca aattttacgc caaagatgag
     1441 aagaatcctt atgattttac catccaatac cgcaagagaa aggaagtcta atggaacgga
     1501 gtttatttgg tctattcacg gcttttctgt gtttcatctg ctttctggca ggagcacaag
     1561 cttttcgtaa aaagcgttac gggctttcta tcctcctttg gttaaatgcc tttaccaatc
     1621 tggtcaatag tattcatgct ttttacatga ccttatttta gatagaatga cagtatagaa
     1681 tggaaggaaa tcatgtctac aaatagaaaa aatgatatga tggtttattg ctcattttgt
     1741 ggcaaaaacc aagaagaagt acaaaaaata attgctggca acaatgcttt tatttgtaat
     1801 gaatgcgtgg agttagctca ggaaatcatt cgagaagaat tggttgagga agtcttggca
     1861 gacttgtctg aggtgccaaa accaattgaa ctcctccata tcttgaacca ctatgtaatt
     1921 ggtcaagatc gtgccaagcg tgccttggca gtggcggttt ataaccacta caaacgcatc
     1981 aatttccacg atacacgcga agagtcagaa gatgtggatt tgcagaagtc aaacattttg
     2041 atgattggcc caactggttc agggaaaact ttccttgccc agaccttggc taagagcttg
     2101 aatgtacctt ttgctattgc ggatgcgaca gctctgacgg aggctggtta tgtgggtgag
     2161 gatgtggaaa atatcctcct caaactcttg caggttgctg actttaacat cgaacgtgca
     2221 gagcgtggca ttatctatgt ggatgaaatt gacaagattg ccaagaagag tgagaatgtg
     2281 tctatcacac gtgatgtttc tggtgaaggg gtgcaacaag cccttctcaa gattatcgag
     2341 ggaactgttg ctagcgtacc gcctcaaggt ggacgcaaac atccacaaca agagatgatt
     2401 caagtggata caaaaaatat cctcttcatc gtgggtggtg cttttgatgg tattgaagaa
     2461 attgtcaaac aacgtctggg tgaaaaagtc atcggatttg gtcaaaacaa taaggcgatt
     2521 gacgaaaaca gctcatacat gcaagaaatc atcgctgaag acattcaaaa atttggtatt
     2581 atccctgagt tgattggacg cttgcctgtt tttgcggctc ttgagcaatt gaccgttgat
     2641 gacttggttc gcatcttgaa agagccaaga aatgccttgg tgaaacaata ccaaaccttg
     2701 ctttcttatg atgatgttga gttggaattt gacgacgaag cccttcaaga gattgctaat
     2761 aaagcaatcg aacggaagac aggggcgcgt ggacttcgct ccatcatcga agaaaccatg
     2821 ctagatgtta tgtttgaggt gccgagtcag gaaaatgtga aattggttcg catcactaaa
     2881 gaaactgtcg atggaacgga taaaccgatc ctagaaacag cctagaggtg actatggaac
     2941 ttaatacaca caatgctgaa atcttgctca gtgcagctaa taagtcccac tatccgcagg
     3001 atgaactgcc agagattgcc ctagcagggc gttcaaatgt tggtaaatcc agctttatca
     3061 acactatgtt gaaccgtaag aatctcgccc gtacatcagg aaaacctggt aaaacccagc
     3121 tcctgaactt ttttaacatt gatgacaaga tgcgctttgt ggatgtgcct ggttatggct
     3181 atgctcgtgt ttctaaaaag gaacgtgaaa agtgggggtg catgattgag gagtacttaa
     3241 cgactcggga aaatctccgt gcggttgtca gtctagttga ccttcgtcat gacccgtcag
     3301 cagatgatgt gcagatgtac gaatttctca agtattatga gattccagtc atcattgtgg
     3361 cgaccaaggc ggacaagatt cctcgtggta aatggaacaa gcatgaatca gcaatcaaaa
     3421 agaaattaaa ctttgacccg agtgacgatt tcatcctctt ttcatctgtc agcaaggcag
     3481 ggatggatga ggcttgggat gcaatcttag aaaaattgtg aggaaaagaa aatggcaaaa
     3541 acaattcata cagataaggc cccaaaggct atcgggccct atgttcaagg aaaaatcgtt
     3601 ggcaaccttt tgtttgctag cggtcaagtt cccctatccc ctgaaactgg ggaaattgta
     3661 ggagagaata tccaagaaca gacagagcaa gtcttgaaaa acatcggtgc tattttggca
     3721 gaagcaggaa cagactttga ccatgttgtc aaaacaactt gtttcttgag cgatatgaac
     3781 gactttgttc cttttaatga ggtttaccaa acggccttca aagaggaatt cccagctcgt
     3841 tcagctgtgg aggtagctcg tcttcctcgt gatgtaaaag tcgaaattga agtcatcgca
     3901 gagattggat aagctagttg aagtttggtg ttgccaaact tcttttgata taaggagaaa
     3961 aagatgacaa agaaacaact tcacttggtg attgtgacag ggatgagtgg cgcagggaaa
     4021 actgtagcca ttcagtcctt cgaggatcta ggttatttca ccattgataa tatgccgcca
     4081 gctctcttgc ctaagttttt gcagctggtt gaaattaagg aagacaatcc taagttggcc
     4141 ttggtagtgg atatgcgtag ccgttctttc ttttcagaga ttcaagctgt tttggatgag
     4201 ttggaaaatc aagatggttt ggatttcaaa atcctctttt tggatgcggc tgataaggaa
     4261 ttggtcgctc gttacaagga aaccagacgg agtcacccac tagcagcaga cggtcgtatt
     4321 ttagatggaa tcaagttgga acgtgaactc ttggcacctt tgaaaaatat gagccaaaat
     4381 gtggtggata cgactgaact cactccacgt gagctgcgca aaacccttgc agagcagttt
     4441 tcagaccaag aacaagccca gtctttccgt atcgaagtca tgtctttcgg atttaagtat
     4501 ggaatcccga ttgatgcgga cttggtcttt gatgtccgtt tcttgccaaa tccctattat
     4561 ttaccagaac tgagaaacca aacgggtgtg gatgaacctg tttatgatta tgtcatgaac
     4621 catcctgagt cagaagactt ttatcaacat ttattggcct tgattgagcc gattctgcca
     4681 agttaccaaa aggaaggtaa gtccgttttg accattgcca tgggatgtac gggtggacaa
     4741 caccgtagtg tggcatttgc taaacgcttg gtgcaggact tatccaagaa ttggtctgtt
     4801 aatgaagggc atcgcgacaa agaccgcaga aaggaaacgg taaaccgttc atgagaaaac
     4861 caaagataac ggtgattggt ggagggactg gaattcccgt cattctaaaa agtctgcggg
     4921 aaaaagatgt ggaaatcgca gctatcgtga cggtggcaga tgatggtggt tcttcaggtg
     4981 aactccgaaa aaatatgcaa cagttgacac cgccaggtga tcttcgtaat gtccttgtgg
     5041 ccatgtcgga tatgcctaag ttttatgaga aggtctttca gtatcggttc tctgaggatg
     5101 ccggagcctt tgctggccat ccattgggaa atctcatcat tgctggcttg tcagaaatgc
     5161 agggttcaac ctataatgcc atgcagttat tgagcaaatt tttccataca acagggaaaa
     5221 tttatccttc cagtgaccat cctttgaccc ttcatgcagt ctttcaggat gggacagaag
     5281 tggctggaga gagtcatatt gtagaccatc gaggcataat tgacaatgtc tatgtgacca
     5341 atgccctaaa cgatgatacg cctctggcca gccgtcgagt agtgcagacc atccttgaaa
     5401 gtgacatgat tgtcctaggg ccaggttccc tctttacctc tattttgccc aatatcgtga
     5461 ttaaggaaat tgggcgggct cttttggaaa ccaaggcaga aattgcctat gtctgcaata
     5521 tcatgaccca acgtggggag acggaacact ttacagatag caaccacgtg gaagtcttgc
     5581 atcgtcacct tggtcgccct tttatcgaca ctgtcttggt gaatattgaa aaagtgcctc
     5641 aggaatacat gaattccaac cgttttgatg aatacttggt gcaagtggaa cacgattttg
     5701 taggtctttg taagcaagtt tcgcgcgtga tttcatctaa cttccttcgt ctggaaaatg
     5761 gcggtgcctt ccacgatgga gatttgattg tggacgagtt gatgcgcatt atacaggtga
     5821 aaaaatgagt ttcacagtag cagtaaaaga agaaatcctg ggccaacacc atctgagctg
     5881 gcatgaatta tctgccatta tcaaaatgtc tggtagcatc ggtctctcga cttcgggctt
     5941 gactttgtca gttgtgacag aaaatgccaa actggcccgt cacctctatg agtcctttct
     6001 ccatttctat gaaatcaaat ctgaaattcg tcaccaccaa cggagcaatc ttcgcaagaa
     6061 tcgcgtctat accgttttta cagatgaaaa ggtgcaggat ttgttaagtg atttgcactt
     6121 ggcagactct ttctttggcc tggaaacaga tattgatgag gcgattttat cggatgagga
     6181 agcaggtcgt gcctatctct gtggcgcttt cttggcaaat gggagcattc gtgaccctga
     6241 gtcaggcaag taccagttgg aaatcagttc tgtttatctg gaccacgcgc aaggaattgc
     6301 ctcccttctc caacagtttt tactggatgc caaggtgctt gagcgcaaga agggggctgt
     6361 gacctatctc cagcgagcag aagacattat ggacttcttg atagtaatcg gagccatgca
     6421 ggcacgtgat gattttgagc gggttaagat tttgcgagaa acccgtaacg acctcaatcg
     6481 ggctaataat gccgagacag ctaatatcgc tcggacagtt tctgccagca tgaagatcat
     6541 taacaatatc agtaaaatca aagatatcat gggcttagaa aatttgccag tagatttgca
     6601 ggaagtggcg caactgagga ttcagcaccc agactactct atccagcagt tagcagatag
     6661 tctcagcacc cctctgacca aaagtggtgt caatcacaga ctcagaaaaa tcaataagat
     6721 agcggatgaa ttataaaacc acgaatccta tgtgactcgt ggttcttttt tataaactag
     6781 tagagtgttt tggttgtact ttttgttcag ggtcaatgta gttgatagcg ttgttgacag
     6841 cagttggagc ttctccgagg cctgtcgcaa tcagatcaat ttttccgtca tagtagcagc
     6901 agtcaccgat agcatagata cctgcttggc tggattcctg tttgctgttg acgataatct
     6961 tgtgacggtt gaggtcgagc ccccagtttt taaggttacc gacagaagat ttgaaaccat
     7021 agttgacaaa gaggtggtct aggtcaatgg tttcagtttc atcagatttg acttttgtga
     7081 tttcaagttt atcaagtgtt tttccatttc caaggagttg gctaggggcg aatggtgtct
     7141 tgatggttac agatgattct tgcaaggctt gaacactgtg ttccaaggca cggaaattat
     7201 ctctgcggtg aacaagggta gttggtgcga ttttttcaaa agccaaagtc caatccacag
     7261 ccgagtctcc cccaccaaga atcgtcactt tcttaccagc gtattgctga atgttagaaa
     7321 cgtggtagtg gatattttca tagccctcaa ccccttcaag ttccagcgga cgtggtttga
     7381 aggcaccgcc acccatagcg atgatgactg ttttagtcag gtgacttcct ttagaagttg
     7441 tgatggcaaa ttcttcttct tgtttgtcaa tctcaagaac cgtttcattg agatgaatag
     7501 gggtatcaaa tccatttagc tgttcaatca agcggttagt caactcttct ccagtcaggt
     7561 ttgggaagcc tggtacgtct aggatttcct tttcagggta gagaatagca ggttgtccac
     7621 ctagctgggg aagagagtcg atgatttgaa ccttggcttg gcgtaggtgg gcataaaagg
     7681 ctgcaaaaag cccgacagga ccaccaccca caatggtaat atcatagagt tgagacatag
     7741 tttctccttt gttttttcta gtcagtttat tttatcatat ttttgttcaa tgtaaaatgg
     7801 actaggatag ggaaaaaaat gccagaaaat ggtataatag gacgaaagtg ttttgatagg
     7861 gaggatatca gggatgaatt ttcaacaatt atccaatctg caatattgga ccagtttgtt
     7921 tgcaagtcca tggacgatag ctatcaatct gattgatatt ttgattgttg cttatatttt
     7981 ataccatttt acaaaagcta ttgcaggaac caagattatg attttggtac gtggagtttt
     8041 ggtgtttatt ttagctcaaa tccttgcaaa tatgattggt ttgactacga tttcttggtt
     8101 aatcaatcaa attattactt atggggttat tgcggcggtt gttatcttct ctccagagat
     8161 tcggactggt ttggaacgtt tgggaagagc gacagatttc ttttccaatg cccctattag
     8221 tgctgaggaa cagatgattc gtgcctttgt taagtctgtt gaatacatga gtcctcgtaa
     8281 aatcggggcc ttggttgcta ttcagcgtgt acgtaccttg caggagtata tttcgacagg
     8341 aattcccttg gatgctaaga tttctgcaga acttctcatt aacattttta ttcccaacac
     8401 tcccctacat gatggtgcgg tgattatcaa agaagaacgt atcgctgtga cgtctgccta
     8461 tctgcccttg acaaaaaaca cagggatttc caaggaattt gggaccagac accgggcggc
     8521 tatcggttta tcagaagtct cagatgcctt gacttttgtc gtatcagagg aaacgggagg
     8581 aatttcgata acctataatg gaaggtttaa gcacaaccta acacttgatg aatttgaaac
     8641 agaattacgt gaaatcttac ttccaaaaga ggaagtgggt cttagtttta aagaacgatt
     8701 gctaggagga tggaaacatg aaaaaaaata gtttatatat catatcctca ctcttttttg
     8761 cttgtgtctt atttgtctat gctacggcga cgaattttca aaacagtacc agtgctaggc
     8821 aggtaaaaac ggaaacctat actaatacag taacaaatgt ccctattgac atacgctata
     8881 atagtgataa gtattttatt agcggttttg cttcagaagt atcagtggtc ttgactggtg
     8941 caaatcgcct atcgctagct agtgaaatgc aagaaagtac acgtaaattc aaggttactg
     9001 ctgacctaac agatgccggt gttggaacga ttgaagttcc tttgagcatt gaagatttac
     9061 ccaatgggct gaccgctgtg gcgactccgc aaaaaattac agtcaagatt ggtaagaagg
     9121 ctcagaagga taaggtaaag attgtaccag agattgaccc tagtcaaatt gatagtcggg
     9181 tacaaattga aaatgtcatg gtgtcagata aagaagtgtc gattacgagt gaccaagaga
     9241 cattggatag aattgataag attatcgctg tcttgccaac tagcgaacgt ataacaggta
     9301 attacagtgg ttcagtacct ttgcaggcaa tcgaccgcaa tggtgttgtc ttaccggcag
     9361 ttatcactcc gtttgataca ataatgaagg tgactacaaa accagtagca ccaagttcaa
     9421 gcacatcaaa ttcaagtaca agcagttcat cggagacatc ttcgtcaacg aaagcaacta
     9481 gttcaaaaac gaattaaaaa tagaaaagga ttttataaca atgggtaaat attttgggac
     9541 tgatggagtc cgtggagaag ctaacctaga actaacacca gaattagcct ttaaactagg
     9601 acgttttgga ggctatgttc ttagtcaaca tgaaacggaa gcgccgaaag tctttgtagg
     9661 acgtgacaca cgtatttcag gggaaatgct ggaatcggcc ttggtggcag gtctcctttc
     9721 agtagggatt cacgtataca aacttggtgt ccttgcaaca ccagcagtag cttacttggt
     9781 tgaaactgaa ggagcaagtg ccggtgtcat gatttctgct agccacaacc cagcccttga
     9841 taacggaatc aagttctttg gcggtgatgg cttcaaacta gatgatgaaa aagaagcaga
     9901 aattgaagcc ttgctagatg ctgaggaaga cactcttcct cgtccaagtg cagaaggctt
     9961 aggaattttg gtagattatc cagaaggctt gcgtaagtat gaaggatacc ttgtttcaac
    10021 tggaactcct cttgatggaa tgaaggttgc cttggataca gctaatggag cagcttctac
    10081 cagtgcccgt caaatctttg cagaccttgg tgcccaattg acggttatcg gggaaacacc
    10141 agacggtctt aacatcaacc ttaatgttgg ttcaacacat ccagaagccc ttcaagaagt
    10201 ggtcaaagaa agtgggtcag ctattggttt ggcctttgat ggagacagtg accgcttgat
    10261 tgctgttgat gagaatggtg acatcgtcga tggtgacaag attatgtaca tcatcggaaa
    10321 atacctttct gaaaaaggac aattggctca aaatacaatt gtgacaactg ttatgtctaa
    10381 ccttggtttc cacaaggcct tgaatcgcga aggtattaac aaggcagtta ctgcagttgg
    10441 tgaccgctac gttgttgaag aaatgagaaa atcaggctac aaccttggtg gtgaacagtc
    10501 tggtcacgtt atcttgatgg attacaatac cacaggtgat ggtcaattat cagcagttca
    10561 attgactaaa attatgaagg aaactggtaa gagcttatca gagttggcgg cagaagtaac
    10621 gatttatcca caaaaattag ttaatatccg agtggaaaac gtcatgaagg aaaaggccat
    10681 ggaagtgcca gctatcaagg ccatcatcga gaagatggaa gaagaaatgg cggggaacgg
    10741 ccgtatcctt gttcgtccaa gtggaacaga gcccctcttg cgtgttatgg cagaagcgcc
    10801 tacaacagaa gaagtggact actatgttga taccatcaca gatgtagttc gtgctgaaat
    10861 tgggattgac taaatcctag aaaactagat gaaaataaaa aattatggta caatagctta
    10921 taatattgta gccgatggag aaagacatga atcttaaacg tgaacaagaa tttgttagtc
    10981 agtatcattt tgatgcgcgt aattttgaat gggaaaatga aaatggagct cctgaaacca
    11041 aggtggatgt gaatttccaa ttgctccaac atgaccaaga aaatcaagtg acttccttga
    11101 ttgtcatctt gagctttatg attgtatttg ataagtttgt cattagtgga acaatctcac
    11161 aagtaaacca tatcgatggc cgtattgtca atgaaccaag tgaattgaac caagaagagg
    11221 tggaaacctt ggctcgtcca tgtttgaaca tgctcaatcg tttgacttac gaagtgactg
    11281 aaattgcatt agacttacca ggaattaatt tggagttcta atatgaaatt agctgttttc
    11341 acagattcct ctgcttatct cagtgaagag accttgcaaa gagaagactt gtttgtcttg
    11401 gatattcctg tcaatattga tggtgaggaa tatgtcgaag gcatcaatct gtctgctgag
    11461 gaattttacc aaaaaatggc tcaggcttct gaattgccta agaccagtca accaagtatt
    11521 gccaagttag atgagatctt aacttcgctc aaagaacaag gctatacaca tgccttgggg
    11581 cttttcctat cttctggaat ttcaggtttt taccaaaata tccagtatat ggtcgatgac
    11641 tatgagggct taaccattgc tttcccagac actttgatta caagtgctcc cctaggcatc
    11701 atggttgaaa gcgtctttaa ttggcgtgat cagggcgatg attttgccag cattcaggat
    11761 aagctagcca ttcaaatcag ccgtacgtca gcctttatca tggtagatga cttggatcat
    11821 ttggtaaaag gtggacgcct ttcaaatggg gctgccattt tgggcaattt gcttagcatt
    11881 aagccaatcc tttattttaa cgatcaaggt gtgattgaag tttacgaaaa agttcgtact
    11941 gaaaagaagg ccaccaagcg cttaattgaa attatcaagg aaacaacggc ttcaggtcaa
    12001 taccgggtca ttgtcattca tgggaatgct cctgaaaagg ctgaagaatt gcgtcagcac
    12061 ttgcttgatt ttggcttggg ttcggatgtt tcacttgcta catttggtag tgtcattgga
    12121 acgcacctag gagcaggaag tattgctctg ggttatattc cagtgattta gttggcgctt
    12181 ctaggctagt taagatgcct tgtatcttag aaattgaaca cgcctggaac cctgtgtgaa
    12241 aaagatagtt cttccaagaa gtagacactt cttgattgga attcctattt tcactttgtg
    12301 ttcttacgga ctttgtatct tagacaggag tagagatgag tattcgagta attattgccg
    12361 gttttaaggg aaagatgggc caggctgctt gtcagatggt attgactgat ccagacttgg
    12421 acttggtggc agttttggat ccttttgagt ctgagtcaga atggcagggt attcctgttt
    12481 tcaaggataa ggctgattta gctggttttg aagcggatgt ctgggtagat tttactactc
    12541 cagctgttgc ctacgaaaat acacgttttg ctcttgaaaa tggctttgct ccagtagttg
    12601 gaacgactgg tttcacgagt gaagaaattg cagagctaaa agaattttct cgtgcccaag
    12661 acttgggtgg cctgattgcc cctaactttg ccttgggtgc tgtcttactc atgcaatttg
    12721 cgacgcaggc tgccaaatat ttcccaaatg tggagattat tgagctccat catgacaaga
    12781 aaaaggatgc tccgagtgga acagccatta aaacagctga gttgatggca gaggttcgag
    12841 agtccattca gcaaggcgca gcagatgagg aagagctgat tgctggtgct cgtggtgctg
    12901 actttgatgg tatgcgcatc cactcagttc gtttgccagg tttggtagcc catcaggaag
    12961 tcatctttgg caatcaggga gaagggttga ccctccgtca tgactcctat gatcgcatct
    13021 ccttcatgac aggagtcaat ttgggaatta aagaagttgt caagcgtcat gagcttgtct
    13081 atggattaga acacttatta tgagattaac gcaaatgcct tctgaatttc agaaggcttt
    13141 accagtatta gaaaaaatta aagaagcagg ctttgaggct tattttgttg ggggctctgt
    13201 tcgagatgcc cttctccata accctatcca cgatgtggat attgcgacgt cttcttatcc
    13261 agaagagacc aagcagattt ttccgcgaac agccgatatc ggaatcgagc atggaaccgt
    13321 cttggtctta gatggggatg aggagtatga ggtaacaacc tttcggacag aggatgtcta
    13381 tgtggactat cgcagaccca gtgcggtttc ctttgtgcgc tcgctagaag aagacctcaa
    13441 acgccgtgat ttcacagtca acgcctttgc cttggatgag acaggagaaa tcgttgactt
    13501 gttccatggt ttagaagatt tggaaaagca agtcttgcga gcagttggag tggctagtga
    13561 gcgtttcaac gaagatgctt tacggattat gcgtggtttc cgttttcagg ctagtcttgg
    13621 ttttgcactt gaaccagaaa catttaaagc tatgaagacc ttgacgccgc ttttggagaa
    13681 aatttctgta gagcgtacct tcgtcgagtt tgataaactc ttgctggctc cattttggag
    13741 aaggggcttg gcttccatga ttgagagtca agcttatgac tatctccctg atatggcatc
    13801 tagccaggac aagctcaaca gactgtttga tttggagact gattttattt ttgaatcctc
    13861 tgaacaagcc tgggcggctt tactatgggc tttggagatt gaaaatgcgc agtcattttt
    13921 gaaatcttgg aagacctcac gccagtttgc caagcaagtt caggatttgc tgattatttt
    13981 ggctctgcgt gaaaatggag aattgagcaa gcgagattgt tatcgctttg acatagattt
    14041 gcttttacag gctgaaaatc ttcgtcaagc tcaaggaaaa gaagtcaacc cacaagccat
    14101 cacagaaaaa taccaaagct tgactatcca tgacaagaaa gagattcaga ttaatggcgg
    14161 tattttgatc aaggaatatg gctatcagcc aggcccagac ttgggagaga ttttaacaga
    14221 gattgagttt gccattgtcg atggagaatt ggagaataat cgtgaagcca tccatgctta
    14281 cctgagggag aaaaaatgag tgattttatc gttgaaaaac taagtaaatc cgttggtgac
    14341 aagaccgttt ttagggatat ttcctttatt atccatgact tagacagaat tggtttaatc
    14401 ggtgtcaatg ggactggcaa gaccaccctt ttggacgtcc tttctggtgt ttctggattt
    14461 gatggggatg tcagtccttt ttcagctaaa aatgattacc agattggtta cttgactcag
    14521 gatcctgatt ttgatgatag aaagacagtt ttggatacgg ttctatctag tgaactcaag
    14581 gaaatccagc tcattcgtga gtatgaattg attatgctcg actatagtga ggacaagcag
    14641 gcgcgtttgg aacgtgtcat ggcagagatg gactctctcc aagcttggga aatcgaaagt
    14701 caggtcaaga ccgttcttag caaattgggc attcaagact tatctactcc tgttggggaa
    14761 ttgtcaggtg gtctgagaag acgggtacag ttggcacaag tcttacttgg caaccacgat
    14821 ctcttgcttt tggatgagcc gaccaaccat ctggatattg cgattattga gtggctgacc
    14881 ctctttttga aaaattctaa gaagaccgtc ctttttatca ctcacgatcg ttatttctta
    14941 gacgctttgt caacacggat tttcgagttg gatcgtgcag gcttgaccga gtaccaggga
    15001 aattaccagg actatgttcg cctaaaggcg gaacaggatg agcgcgacgc ggctcttctt
    15061 cacaaaaaag aacaactcta caaacaagaa ttggcctgga tgcgcagaca accgcaggcg
    15121 cgtgcgacca agcagcaggc ccgtatcaat cgattccatg atctgaaaaa ggaagtttca
    15181 ggcagtagtg ctgagacaga cttgaccatg aactttgaaa ccagtcggat tgggaagaaa
    15241 gtcatcgagt ttcaggctgt ttcctttgcc tatgaaaaca agcctatttt gcaagatttt
    15301 aatctcttgg tgcaggccaa agaccgtatc ggaatcgttg gggacaatgg tgttggaaaa
    15361 tcaaccctgc tcaacttgat tgcaggaagt cttgagccaa ctaaaggaca agttgtgatt
    15421 ggggaaactg ttcgcatcgc ctatttctct cagcaaattg agggtttgga tgaaagcaag
    15481 agagttatca attacctgca ggaagtggca gaagaggtca agaccagtgg cggttctacg
    15541 acttcgattg cagagttgct ggagcagttc cttttcccac gttcgacgca tggaaccttg
    15601 attgagaaat tgtcaggggg tgagaaaaaa cgtctttatc tcctcaaact gcttttggaa
    15661 aaaccaaatg ttcttctttt agacgagcca accaatgacc tagatattgc aactttgaca
    15721 gtcttagaga atttcttgca aggttttgca ggtcccgttt taacagtcag tcacgaccgc
    15781 tatttcttgg ataaggtagc gaccaagatt ctcgcttttg aggatggcaa gattcgtcct
    15841 ttctttggtc attacaccga ctatcttgat gaaaaagctt ttgaaacaga tatggccaat
    15901 caagtgcaaa aggtcgaaaa ggaaaaagtg gtcaaggttc gagaagacaa gaaacgcatg
    15961 acctaccaag aaaagcagga gtgggcaagt attgaaggtg atattgaaac cttggaaaaa
    16021 cgtatcgctg ctattgaaga ggaaatgcag gctaacggct ctgactttgg taagctggct
    16081 actctccaaa aagaattgga tgagaaaaat gaagcactcc ttgaaaaata cgaacgctat
    16141 gagtatctca gtgaatttga tagttaagac tattcgaaaa tcaaagtgca aactatgtcg
    16201 gcttcacatt gccgactcaa gtacagcctg tgaccggctt cctaatttgc actttgattt
    16261 ttattgagta taaacgaata gaaaaatccc gtctcatgaa aggatttctc tattcttttt
    16321 tggtttcttg atgaaagata ttttgccagg tttcgtggcg acgccagata ctggtatgga
    16381 tgataccatc taactcatag cagataagtt tggttttttg actgatggaa gtaatttgaa
    16441 tgtccttgat agcagaattt aactcttttt tggtcttata agcctcttta tccatctgct
    16501 ctccatcttg gcgaatatag acaaaatcat cagtcaagag ttcttctagt tggtttcctt
    16561 ggtcgatcaa ttgttcacgc ataagcaatt ttttatagac attgtctaaa atttcatcct
    16621 cagggatagg tgctggttcg atatggacat cggtatcaaa gacgccaaaa cgctcctcca
    16681 gcatagactc gacctgatcc gcgatttcat ggctttcaaa aacagacaag tcaggattca
    16741 tctctagtgt aatatccagg tagatgttgc taccgtaggt gcgacctctt tgcgatttga
    16801 ccttgctgat tttgggaatt tccatgatag ctttttggta gtcctcgagc aggcggtcgt
    16861 caaagccatc tgaaagacta aaggaagact cgatgaagat atcataggca gtcttcaaga
    16921 taaagaaagt gatgatgata gcaaccagtt tatccacaat cggataattg aaactactag
    16981 ctaggatggc aatggcggtt ccaagtgagg taacagcgtc agaaagattg tccttagcag
    17041 ctgccttcag cgccttggag ttggatttct tactgaggcg agtattgtag agatagacca
    17101 caaacataat cgctgcagaa atgattccta gagttgcacc aagaggatca atgaccgttt
    17161 cttcccgact gagaatcttt tgaatggtat ctcttagaac atcgaaaccg acatagaaca
    17221 tgatgataga agtgatcaag cttgccaaat cttcaatctt ccaatgacca aaacggtggt
    17281 cacggtctgc aggctggcgc gccatccgaa tcccgattaa gagggccaca tttccaatga
    17341 tgtccgatac gttattaaaa ccatcggcca ccaaactgga tgaatgaagg agatgaccag
    17401 ctgctaattt ggctgcagac aagatcaaat aggtcgaaat actgataatg gctccacgct
    17461 cagctaactt gagatttgag atagattgct tcattcaact tcctttctag tcacatagca
    17521 ttctaccatt atactggttt tcaaggaaaa attcaaataa ggcagttttt actctttgaa
    17581 aactccccaa aatttggtat aatggtacta ctcaagggag tagctggcag aaacctgtga
    17641 tagtgtcgtc attccgaatt ttatgctgaa aagtatgctt tccggcccta tcttaaacag
    17701 cgagacttgt tatgattaac aggtctcttt ttgtttgtcg tgaaaagata tgacagaaag
    17761 agagtctgtt ttgcacacaa tgtcaaggag gagacacatg tcaaaagaac aaaaacgcca
    17821 agcgttttat actcagagcc ctgaagaggt cttgcaggct gtggatgcga ccgagcaagg
    17881 tttgtcatca agtgaggcgg aaaagcgcct tgccgaattt gggcacaatg aactcgaaga
    17941 aggcgagaaa cgatcaatct tggtcaaatt catcgagcaa tttaaggatt tgatgattat
    18001 catcctagtt gcggcagcaa tcttgtcagt cgtgacttct ggtggggaag atatcgcaga
    18061 tgccattatc atcctagctg tggttatcat caacgctgcc tttggtgttt accaagaagg
    18121 aaaagctgaa gaagctattg aagccctcaa atccatgtct agtccagttg cccgcgttct
    18181 tcgtgatgga cacatggcag agattgactc taaagaattg gtaccaggcg atatcgttgc
    18241 ccttgaagca ggtgacgtgg taccagcgga cctacgtttg atagaagcca actctcttaa
    18301 aattgaagaa gcagccttga caggtgaatc tgtaccagtc gaaaaagact tgtcagtcga
    18361 tcttgcgaca gatgctggca ttggtgaccg tgtcaacatg gccttccaaa actcaaacgt
    18421 aacctatggt cgtgggatgg gtgttgttgt caatacaggt atgtatactg aagttggtca
    18481 tattgctggt atgcttcaag atgcggatga gactgataca ccactcaaac aaaacttgaa
    18541 caacctttct aaggtcttga cctatgctat cttggtcatt gcccttgtta cttttgtagt
    18601 gggtgtcttc attcaaggga aaaatccact tggtgagttg ttgacttctg ttgcccttgc
    18661 cgttgcagcc attccagaag gacttcctgc tatcgttacc atcgttcttt ctcttggtac
    18721 tcaagttttg gccaaacgac attccatcgt tcgtaagttg ccagcagttg aaacacttgg
    18781 ttcaactgaa atcatcgctt ctgataagac tggtacgctg actatgaaca agatgacagt
    18841 cgaaaaagtc ttctacgatg cggttctaca tgactcagct gatgatattg aactaggtct
    18901 tgaaatgcca ctacttcgtt cagttgtctt ggccaatgat acgaaaatcg atgtggaagg
    18961 taacttgatt ggtgacccaa ccgaaacagc ctttatccaa tatgccttgg acaagggcta
    19021 tgatgtcaaa ggtttcttag agaaatatcc tcgtgtagct gaattgccat ttgactctga
    19081 ccgtaagctc atgtcaacag ttcacccatt gccagatggt cgtttccttg tagcagtcaa
    19141 gggtgcgcca gaccaactct taaaacgttg tcttcttcgt gataaggctg gggatattgc
    19201 tccgattgat gagaaggtta caaatctcat tcatacaaac aattctgaaa tggctcatca
    19261 agccttgcgt gtccttgcag gtgcttataa gattatcgat agtattccag aaaatctcac
    19321 ttctgaagag cttgaaaatg atttaatttt tactggtttg attgggatga ttgaccctga
    19381 acgtcctgaa gccgctgagg ctgttcgtgt ggctaaggaa gcgggaatcc gtccgattat
    19441 gattacaggt gaccaccaag acacagcgga agccattgcc aaacgtttgg gaatcattga
    19501 cgcaaacgat acagaaggtc acgttttaac tggtgctgaa ctcaatgaac tgtcagatga
    19561 agaatttgaa aaagtcgttg gtcaatactc tgtttatgcc cgtgtgtctc cagaacacaa
    19621 ggttcgtatc gtcaaggctt ggcaaaaaca aggtaaagtc gttgccatga caggtgacgg
    19681 tgtcaatgac gcgccagctc tgaaaacagc cgatatcggt atcggtatgg gaatcactgg
    19741 tacagaggtt tctaaggggg cttctgatat gattcttgca gatgataact ttgcgactat
    19801 tatcgtcgca gtggaagaag gacgtaaggt cttctcaaac attcaaaaga ctattcagta
    19861 cctactttct gctaatactg ctgaagtatt aaccatcttc ctatcaacct tgtttggttg
    19921 ggatgtcttg cagccggttc atcttttgtg gatcaacttg gtaacggata ccttcccagc
    19981 tatcgctctt ggtgttgaac ctgcggagcc tggtgtcatg aatcataaac cacgtggacg
    20041 caaggcaagc ttcttctcag gtggtgtttt gagttctatc atttatcaag gtgtactcca
    20101 agcagctctt gttatgagtg tttatggcct tgcgattgct tacccagttc atgtgggtga
    20161 caatcatgct attcatgcag atgccctaac gatggccttt gcaacccttg gtttgattca
    20221 gctcttccat gcctacaatg tcaagtctgt ctaccaatcc atcttgacag ttggcccatt
    20281 caagtctaag acctttaact ggtccatctt ggtatccttt atccttctca tggcaacaat
    20341 cgttgtagaa ccgcttgaag gaatcttcca cgtaaccaaa ctagacttgt cacaatgggg
    20401 aattgttatg gctggaagct tctcaatgat tatcatcgtc gaaatcgtta agtttattca
    20461 acgcaaactc ggttttgaca agaatgcgat ttaagggagg tttcggatgt cagcatatca
    20521 attaccgacc gtatggcagg atgaagctag taatcaagga gcttttacgg ggctaaacag
    20581 accaacagca ggtgcccgtt tcgaacaaaa cttgccaaaa ggagaacaag cttttcagct
    20641 ttattcactg ggaacaccaa atggtgtgaa ggttactatc ttattggaag aattactaga
    20701 agctggtttt aaggaagcgg cttacgactt gtataagatt gctatcatgg atggggatca
    20761 attcggctca gactttgtga agctcaatcc aaattccaag attccagcct tattggacca
    20821 gtcaggtact gaaaacgtaa gagtctttga gtctgctcat attcttcttt accttgctga
    20881 gaaatttgga gcctttttac caagtaatcc tgtggaaaag gtagaagttt tgaattggct
    20941 attctggcaa gcaggtgcag caccttttct aggtggggga tttggacatt tcttcaatta
    21001 tactcctgaa aaattggaat atcctattaa ccgttttacg atggaagtga aacgccagtt
    21061 ggatttattg gataaggaat tggctcagaa accttatatt gcaggcaatg actatacgat
    21121 tgcagatatt gctatctggt cttggtatgg acagttagtt caaggaaatc tttaccaagg
    21181 ttctgcaaaa ttcttggatg cctcaagtta tcaaaatcta gtaaaatggg cagaaaaaat
    21241 tgccaatcgt ccagctgtta agcgtggctt ggaagtaact tatacagaaa ttaaatagca
    21301 aaaaagtcat cttcggatgg ctttttgcta cagttgaggg aaagggtttg cagttatcgg
    21361 cttttgtgct ataattgcta taatatagta aaaagcaaga ggagtgtgag gaagtggaaa
    21421 agaaaattgt gaaggatatc ttatttttat ctcaagtgtc tcagccggca agtcaggagg
    21481 acctttatct tgccagagat ttgcaggata cactcttagc aaatcgtgat acctgtgttg
    21541 gtctagctgc caatatgatt ggggtgcaga agcgcgtgat tatctttaat cttggcttgg
    21601 ttcctgtggt catgtttaac ccagtgcttc tgtcctttga aggatcctat gaggcagaag
    21661 aaggctgttt gtccttggta ggtgtgagat caactaagcg ttatgaaacc ataaggcttg
    21721 cctatcgtga cagcaagtgg caggaacaga ccattacctt gacaggcttc ccagctcaga
    21781 tttgccagca tgagctggat cacttggaag gacgaatcat ttaggaggaa agcaaatgaa
    21841 acgaatagtc tttgaactta tttttatcgc aacgacctgg tatatctttt taccgcccct
    21901 taacctgacc agctgggaat ttctattctt cctctgtggg catttgttag ttgtggcaat
    21961 cttatttggc tttggcaagg ggataaacct tgtcaaaacg gttcatgtgc gccacggtaa
    22021 ggcggaagct gccttaaatc ttgagggttt caaaatcaat cggttaggga aaattctgtt
    22081 agcttcgatt ggaggaattc ttctcttggc agctttggtt tccttggtaa cttccagcat
    22141 gtttcaggct aaaaattatg ccaatgtagt cacggttacg gaaaaagact ttactgaatt
    22201 tcctaagagt gacaccagta aggttcctat cctagataga agtactgctg aaaaaattgg
    22261 agaccgctac ttgggttccc taaccgataa ggtgtcgcaa tacgtagcgg cagataccta
    22321 tacccaattg acaattgatg ggaaacctta tcgggtcaca ccactagaat atgcagaccc
    22381 tatcaaatgg tttaacaatc aagccaaggg aatcggtgag tatattaagg tggacatggt
    22441 aactggaaat gcggatttgg tggacttgaa gacaccaatc aagtattcag actcggagta
    22501 ttttaaccgt gatgtcaaac gtcacctgcg cttgaagtac ccgaccaaaa tctttaaaac
    22561 tccatctttt gaggtggacg atgagggcaa tcctttctat gtagcaacgg tttaccaaaa
    22621 gcaatttgga cttgctgttc ctcgtcctgc ttcagtcatt atcttggatg ctacaaatgg
    22681 agaaaccaag gaatacagct tatcagatgt tccagaatgg gtggacagga tctatccagc
    22741 agaggaaacc attgagcaaa ttaactacaa cggcaagtac aaggacggtt tcttgaatgc
    22801 catgatttcc aagaaaaacg tgacccagac taccaagggc tataattact tgtctatcgg
    22861 taatgacatc tatctctaca caggtgtgac gtcggctaat gcggatgaga gtaatcttgg
    22921 tttcatcctt gaaaatatgc gaacaggaga aatcactaag tatagcttgg cttctgcgac
    22981 agaagaatca gcccgtgaat cagcagaagg tgctgttcag gagaaatcct acaaagcaac
    23041 cttcccaatc ctcatcaacc tcaatgacaa gcctctctac atcatgggct taaaggacaa
    23101 tgctggcttg gtcaaagagt acgccctggt agacgcagtc gagtaccaaa atgttatcgt
    23161 tgctactaca gtggaagaga tgctcagcaa gtatgccaat aaaaacgacc ttgaaattga
    23221 caatgcaacg acagaaagca tcaagggagt agtagcagac ctcaaatcag ctgttatcaa
    23281 gggagacact gtctacttct ttaaagttga tggcaagata tacaaggtca aggcttcagt
    23341 atccgatgat cttccttacc ttgaaaatgg taaaaccttc gaaggtcaag taggaaaaga
    23401 caattatctc aagaccttta agctacggta aaaataggtt tttttcagaa agtatatgtt
    23461 ataataaggt aaattaagcc taatggagga caaagaaatg ggtttttatt tgatggttgc
    23521 ttcaatgcta ctcggactgc tcgctttgaa gataggtttt tctcagttca aggagaaaaa
    23581 agataaattc ttatctatct taacaagttt agctggcaca gcccttgttc tcgtggctgt
    23641 ctggttagga tggcccaaat aaatagaaaa acctcggtca aaccgaggtt tttcaaatga
    23701 atttcttggt tgtggctaaa aaatatgcta cactatcaat atgaaaattt taatcccaac
    23761 agcaaaagaa atgaacacag acttgccaag tatcgaggca attcctttaa aaccagaaag
    23821 tcaggccgtg cttgatgcct tggctctcta ttctgccagt caattggaga gtttctacaa
    23881 ggtatcagct gagaaagcgg cggaagaatt tcaaaatatc caagctttga aaaggcaaac
    23941 tgctcaacac tatccagcct tgaaactttt tgatgggctt atgtaccgca acattaagag
    24001 agataagctg accgaggcgg aacaagatta tcttgaaaat catgtcttca ttacctcggc
    24061 tttgtacggt gttgttccag ccttgtcacc catggctcct caccgtttgg attttttgat
    24121 gaaattaaaa gtcgctggta agactttgaa gagccattgg aaggcagtct atgatgaagc
    24181 tctgaagaag gaagaagtga ttttctctct cttgtcatca gagtttgaga ctgtattttc
    24241 taaggaaatc agagcaaaga tggtgacctt caaattcatg gaggatagag gcggtcagct
    24301 gaagattcac tcaactatct ccaagaaagc gcgcggggcc tttctaacag ctttaataga
    24361 aaatcaagta caaactgtgg gggaagcacg tcgcttgaac tttgctggat ttgtttaccg
    24421 agaagatttg tcacaaccac agggattggt ttttgtaaag gaagtataga aatcagatat
    24481 tttgtatttg gcaatatgta tcatcagcta ttttagttta taaatgagaa aaaagcgaac
    24541 aagctagtct tctgagtgtc agaaaagtag tcttgttcgc ttttattatg ttatactctt
    24601 cgaaaatctc ttcaagccac gtcagtgtcg ccttaccgta tatatgttac tgacttcgtc
    24661 agttctattt acaacctcaa agcagtgctt tgagtagctt gcggctagct tcctagtttg
    24721 ctttttgaat tttattgagt gttagttact tgatagcttc gaccaagtct tgagcttgtt
    24781 tttcaagtga gtttaggact gtttcttcaa gaaccaattt tccgtctgcc caggcagagt
    24841 cattaacacg tgcagcagtg aaatcaccaa cgccttgtgt acggataaat ggcaagaggt
    24901 ctttgtagat agcgaaaagt tgatcgtgcc ctgcattggc tacagatgat attgtgacaa
    24961 acttgtcttg aagggcagaa gcgccacgtg tatcagacaa gtcaagggca cgagatagcc
    25021 agtcaagcaa gtttttcact gtaccaggga tagagaagtt gtagactgga gagaaaatcc
    25081 agatagcatc cgcaacgaga actgcttcac gagcagcagc tacagctgga tgtgttggaa
    25141 cttccaaatc ttggctgaag agaggaagag ctgaataatc aaggtagcta acttccgctt
    25201 taccagcaag tgctttctca gcttcgagcg ccatttggtg gttgaaagaa ccttgacgta
    25261 gtgatccgac gataaataat acttttttag acatgatgtg tctccttttt cttaaaattt
    25321 cgagaatcct ctcgttgtta taaattatat tacaacaaaa caaaagaact tgcaagaaat
    25381 ttgctcagag tttatttgaa aattgtaaaa tttcggaaac tctagtcatt tgtgactgaa
    25441 aaagtaagaa ctgtttcact ttcaaaatcg gttttgtttg aagggagaaa tagaattggt
    25501 tgctcaaagc acagctttga ggttgcagat aaagctgacg tggtttgaag agattttcga
    25561 agagtattct tatattcact tgtaataaag cttaatagat ggtaaaatag acgagtgaaa
    25621 aaaagacaaa acaaagcaaa aaataatcta ctgtggcagt atggtctagg gatgacgatt
    25681 ttgtttgtgg ttatcagtgc ttcctttctg tatatggttt ctcttagcat gaaaccctat
    25741 caaacagcta aaagtgaagg agaaaaatta gctcagcagt atgcaggatt agagcaggcc
    25801 gatcaggttg atttatacaa tggcttggaa tcttattaca gcgttcttgg tcgtaataaa
    25861 cagcaagaag cacttgctgt tctgattgga aaagatgatc ataagattta cgtttatcag
    25921 ctaaatcagg gtgtttcaca agaaaaagca gaaacggttt ctaaggaaaa gggagctggc
    25981 gaaattgaca agattatctt tggtcgttat caagataagc caatctggga agtcaagtca
    26041 ggatctgatt tttatctagt agattttgaa acaggagcat tggtcaacaa ggagggccta
    26101 tgaaattatc caaccgtgtt ttagaaatgg aagaaagtgt gactttggct gctggagccc
    26161 gtgccaaagc actgaaggct gagggcagag atattttgtc tctaaccttg ggtgagccag
    26221 atttcactac tcccaaaaat atccaagatg ccgccattgc atcgattcga gatgggcgtg
    26281 cttcttttta tacagtaacc tcaggtctgc cagagcttaa ggcggcggtc aatagctact
    26341 ttgagcgctt ttacggctat tctgtagcgt caaatcaagt gatagtcgct gcgggagcca
    26401 aatattctct ctataccttc tttatggctg tggtcaatcc aggtgatgaa gtcatcatcc
    26461 caaccccata ctgggttagc tatggagatc aggtcaagat ggcagaaggc gttcccgtct
    26521 ttgtttctgc taaggaagac aatcacttta aggtgaccgt agagcagtta gaagcagctt
    26581 gcactgacaa gaccaaggtt ttggtgctga attcgccatc taatcccaca ggtatgattt
    26641 acacccgtga ggaactcttg gcaattggaa actgggctgt agaaaatgat attctcatcc
    26701 tagcagacga tatctatggc cgcttggttt ataatggtca tgagttcaca ccgatttcta
    26761 gcctatcgga agcgattcgc aagcaaacag tggtcatcaa tggtgtgtct aaaacctatg
    26821 ccatgactgg ttggcggatt ggttatgccg ttggggaagc agacattatc gctgccatgt
    26881 ccaagattgc aggtcaaaca acttcgaatc cgtcagcagt agcccaatat gcagcagttg
    26941 aggctctatc aggtgagcaa gatactgtag aaagcatgcg tcaggccttt gaggaacgtc
    27001 ttaataccat ctatcccctc cttgcagaag tgccaggatt tgaagtggtc aaaccgcaag
    27061 gggccttcta tctcttccca aatgtcaaaa aggccatgga gatgaaaggc tacacggatg
    27121 tgacagactt tacaactgtt atcttagaag aagccgaagt ggccttggta acaggagctg
    27181 gatttggagc accagaaaat gtccgcctca gctatgcgac agacctagac acgcttaaag
    27241 aagcagtcga acgcttgaaa gcatttatgg gtagtgagaa tgattgatca ttttgagatt
    27301 aaggtaaagg atttacaaat ttcagaagga ttttatagga gttttctcgc tcctttggac
    27361 tataaattgg cttttaagac tagttctcta attagttttc tatccccgaa cagccctcat
    27421 cctggtggtg atttttggct gactcaagga acacaagatc ctgttcattt tgctttttta
    27481 gcagaaaata aggaagaagt ttaggcttgt tatgaggctg gcttagaggc aggtgggcga
    27541 gacaatgggg ctcctggtta tcgaagtgag cgtccgattt actatgctgc ttttatgatt
    27601 gacctggatg ggaacaatat agaggtggtt tgccataaag aataaaaaag aaaaggaaaa
    27661 ataatgacaa aacgtgtaac gattattgac gtaaaagact atgttggtca ggaagtgacg
    27721 attggcgctt gggttgccaa caaatcagga aaagggaaaa tcgctttctt acaattgcgt
    27781 gatggaacag ccttctttca aggtgtggct tttaaaccaa actttgtcga aaaatttggt
    27841 gaagaagtgg gacttgagaa gtttgatgtt atcaaacgct tgagccaaga aacgtctgtt
    27901 tatgtgacag gtattgtcaa agaggacgaa cgttctaaat ttggctatga gttggacatc
    27961 acagacatcg aagtgatcgg tgaatctcaa gactacccaa tcacaccaaa agaacacgga
    28021 acagactttt tgatggataa ccgtcacttg tggctacgct ctcgtaaaca agtagctgtg
    28081 ttgcaaatcc gtaacgctat tatctatgca acttatgagt tctttgacaa gaacggtttt
    28141 atgaagtttg acagcccaat tctttcagga aatgcggcag aagattctac agaactcttt
    28201 gaaactgact acttcggaac gccagcctac ttgagccaat caggtcagct ttacctagaa
    28261 gcaggggcta tggctcttgg tcgtgtcttt gactttggtc cagttttccg tgctgaaaaa
    28321 tcaaaaacac gccgtcactt gactgagttc tggatgatgg atgctgagta ctcatacttg
    28381 acacatgatg agtcgcttga cttgcaagaa gcttatgtga aagctcttct acaaggtgtt
    28441 cttgaccgcg cgcctcaagc cttggaaacc ttggaacgtg atacagaact cttgaaacgc
    28501 tacattgcag agccattcaa acgtatcact tacgatcaag ccattgacct cttgcaagag
    28561 catgaaaatg atgaagatgc tgactacgag catcttgagc atggtgatga ctttgggtca
    28621 ccacacgaaa cttggatttc aaaccacttt ggtgtgccaa catttgtcat gaactatcca
    28681 gcagccatca aggccttcta catgaaacca gttcctggaa atccagagcg cgtgctttgt
    28741 gcagacttgc ttgctccaga aggctatgga gaaattatcg gtgggtctat gcgtgaggaa
    28801 gattacgatg cccttgtcgc taagatggat gaacttggca tggatcgtac agaatatgaa
    28861 ttctaccttg accttcgtaa atacggtaca gttccacacg gaggatttgg tatcggtatc
    28921 gaacgtatgg taaccttcgc agcaggaaca aaacatatcc gtgaagctat tccattccca
    28981 cgtatgttgc accgtatcaa accataagaa caggaaaacg cccatgtggc gttttttcga
    29041 attctagtgt ttttacttgc gtaaaaaaat tttttctagt ataatagttt attgtgagcg
    29101 aacctcactt accccttgca aagtcttggg gtcattagac caaaaggagg aacatatcaa
    29161 tggctaaata cgaaattctt tatatcattc gtccaaacat tgaagaagaa gctaaaaacg
    29221 ctttggtagc acgttttgac tctattttga ctgacaacgg tgcaactgtt gttgaatcaa
    29281 aaacttggga aaaacgtcgt cttgcatacg aaatccaaga tttccgtgaa ggactttacc
    29341 acatcgttaa cgttgaagca aatgacgatg cagctcttaa agagtttgac cgtctttcaa
    29401 aaatcaacgc tgacattctt cgtcacatga tcgtcaaaat tgacgcgtaa gaaggtaaac
    29461 tatgattaac aatgttgtac ttgtagggcg tatgacacgt gacgctgaat tgcgttatac
    29521 cccatcaaat gtagcagttg cgacttttac tcttgcagta aaccgtacat ttaagagtca
    29581 aaatggtgaa cgtgaggctg attttatcaa tgtcgttatg tggcgccaac aggctgaaaa
    29641 tcttgctaac tgggctaaaa aaggctcact tatcggggtg acaggtcgta tccagactcg
    29701 tagttacgat aaccagcaag gacaacgtgt ctacgtgaca gaggtcgtgg ctgagaattt
    29761 ccaaatgttg gaaagccgta gtgtgcgtga gggtcacaca ggtggagctt actctgcacc
    29821 aactgcaaac tattcagcac ctacaaattc agtaccagac ttttcacgta atgaaaatcc
    29881 atttggagca acaaacccat tggatatttc agatgatgat ttaccattct aatggacaac
    29941 taaacttata aaggagaaaa aacatggctc aacaacgtcg tggcggattc aaacgccgta
    30001 aaaaagttga ttacatcgca gcaaacaaaa ttgaatatgt tgattacaaa gatactgagc
    30061 ttcttagccg tttcgtttca gaacgtggga aaatccttcc tcgtcgtgta acaggaactt
    30121 cagctaaaaa ccaacgtaaa gtaacaacag ctatcaaacg cgctcgcgta atggctttga
    30181 tgcctttcgt aaacgaagat taaagaaaag acccagttgg gtctcttttt catgagcttg
    30241 aaaacaagaa agcgaattaa gaccccaacg ggtctttttt cacaagcttt gaaatgagag
    30301 agcgagttag gctcagtggc tcccttttta cgagtttgaa aacaagagaa cacattaaaa
    30361 gcacgatgga gctttttcat aaatatgtct cttttctcat ccaagaaatt aaaataaaaa
    30421 tgataaactt tttgaaaata ggggatttgc ctactttatg atataatgga aagagttaga
    30481 ttgaaagagg tagtgagatg gacgcgaaat taagatacaa ggcaaagaag atcaagattg
    30541 tcttttttga tattgatgat acattgcgga attcaaagac agggtttatt ccaactacaa
    30601 ttcccactgt ttttaaacag ttgcgtgaaa aaggaatttt aacaggaatc gcctctggac
    30661 gtggcatttt tggtgttgtt ccagagattc gtgatctcaa gcctgacttt tttgtaactt
    30721 tgaatggggc ttatatcgaa gataaaaaag gtcaggtcat ttatcagcat cagattgaaa
    30781 agtcagatgt tgaggagtat atctcttggg ctaagcaaga aggaattgag tatggcttgg
    30841 ttgggagtca tgatgccaag ttgtcgactc gcaccgatat gatgagtgaa gctatcaatc
    30901 caatttatcc cgacttagat gtagatcccg atttccatga aaaagaagat atctatcaga
    30961 tgtggacttt tgaagataag ggagatgact tgcacttgcc tgacagtctc tcagacaaac
    31021 ttcgcatggt tcgttggcat caacattcct ctgatattgt gccgatttca ggctccaaag
    31081 cgacgggggt ggaaaaggtt gtggaacacc ttggcttgaa accagagaag gtcatggttt
    31141 ttggagatgg tctcaacgac ttggaactct ttgattatgc tggaatcagc gttgccatgg
    31201 gaatttctca tgataaaatc aaagaaaaag cagattatat tacaaaaaca ttagaagaag
    31261 atggcatttt tgatgcctta gaagtatttg gtatggtaga aaaagaattg cattttccac
    31321 aagtagacat tgaaacagta gaaggtcctc ttgcgactat taagaccaat cacggagact
    31381 tacgtatcaa gcttttccct gaacatgctc ctaaaacagt ggctaacttt gtatctcttt
    31441 caaaagatgg ctactatgat ggtgtcattt tccaccgtat tatcaaggac tttatgatcc
    31501 aaggtggaga cccaactgga actggtatgg gtggcgagtc aatctacggc gaatcttttg
    31561 aggatgaatt ctcagaagag ctttacaata tccgtggtgc tctttccatg gcaaatgctg
    31621 gtccaaatac caacggcagc cagttcttta tcgtgcaaaa ccaacaccta ccttattcta
    31681 agaaagaaat tactcgtggt ggttggccag aaccgattgc agaaatctat gccaatcaag
    31741 gtgggacacc tcacctagac cgccgtcaca cggtttttgg tcagttagct gatgaagcat
    31801 cttacgctgt cttggatgcc attgctgctg ttgagacagg agctatggac aagccagttg
    31861 aagatgttgt aattgaaact attgaaatcg aggactaaga tgaaaatcgg tgataagcta
    31921 aaggggcgta ttacagggat tcagccctac ggtgcctttg ttgagttaga gacgggtgat
    31981 acggggctga ttcacatttc agaaattcgg acaggattta ttgaaaatat tcatgaaacc
    32041 ttgaaagtcg atgaagaggt tcaagttcag gtagtggatt tagatgaatt tacggggaaa
    32101 gccagtcttt ctatccgcac tttggaggaa gaaaagtacc agtttcctag acggagacgc
    32161 ttttcaagcg accgctttaa ctacggtttt gctccctttc gacgaatgct accaatctgg
    32221 actggagaag ccctgcacta tttaaaaaag aagaagtagt ttagagtttt atattttttg
    32281 taatgttaat ataaatttgc tataatagga ccatggaaga agaacaatta ttaaaatcag
    32341 gagagcgcat taaccagctc ttttcgacag atatcaaaat cattcaaaat agagaggttt
    32401 ttagctattc ggtggatagt gttctcttat cacgatttcc acgttttcct aagaaggggt
    32461 tgattgtaga tttctgtgct gggaatggag cagtggggct ttttgctagc actcgtactc
    32521 aagcacagat attgtctgtt gagattcagg agcgtttggc ggatatggct gaacgctctg
    32581 tccgtttgaa tggattagag gagcaaatgc aggtcatctg cgatgatttg aaaaatatgc
    32641 ctgctcacat tcaggggagt aaggtggata tgattttgtg taatccaccc tatttcaagg
    32701 tggatcctca ttctaatctg aacgagagtg aacattatct cttggctcga cacgaaatca
    32761 cgactaattt ggaagaaatc tgtcatagtg cccagagtat tctcaagtct aatgggcgtt
    32821 tggccatggt tcatcgtcct gatcgacttc tggatatttt ggatacgtta aaacgacata
    32881 atctagcccc taagcgcctg cagtttgttt atccaaaaag agaaaaggaa gccaatatgc
    32941 ttttgattga ggcgatcaag gatggctcaa caagtggctt taaggtctta cctcctttga
    33001 ttgtccacaa tgatgatggc tcttatacgc ccgaaatcga agagatttat tatggatcat
    33061 aaggcttata tgtacgttct ggagtgtcgt gatggatcct actatatagg ctatacgact
    33121 gatgtgagaa gacgcctcgc tatccacaat agtgggaagg gagccaaata tacacgagca
    33181 cgcttgccag tcaaacttat ctatgctcaa ggttttgcca gtaaggaaga agccatgtcg
    33241 gctgaagctc ttctcaagcg taagaagagg ccacagaagg aagaattttt atctgaaaat
    33301 caagatagaa atttactcag ttattttgaa gaatcgtggg gagtcctttg actcctctta
    33361 cctttgtcaa aaagcgaaaa aaatgctaca ataaagaaaa taaacaaaat gaggtattat
    33421 catgtccaag attctagtat ttggtcacca aaatccagac tcagatgcca tcggatcatc
    33481 tgtagctttt gcctaccttg caaaagaagc ttacggtttg gatacggaag ctgttgccct
    33541 tggaactcca aatgaagaaa cagcctttgt cttgaactat tttggtgtgg aagcaccacg
    33601 tgttatcact tctgccaaag cagagggggc agagcaagtt atcttgactg accacaatga
    33661 attccaacaa tctgtatcag atatcgctga agtagaagtt tacggtgttg tagaccacca
    33721 ccgtgtggct aactttgaaa ctgcaagccc actttacatg cgtttggagc cagttggatc
    33781 agcgtcttca atcgtttacc gtatgttcaa agaacatggt gtagctgtgc ctaaagagat
    33841 tgcaggtttg atgctttcag gtttgatttc agataccctt cttttgaaat caccaacaac
    33901 acacccaaca gataaaatca ttgctcctga attggctgaa ttggctggtg tgaacttgga
    33961 agaatatggt ttggcaatgt tgaaagctgg taccaacttg gctagcaaat ctgctgaaga
    34021 attgattgac atcgatgcta agacttttga actcaacgga aataatatcc gtgttgccca
    34081 agtgaacaca gttgacatcg ctgaagtttt ggaacgccaa gcagaaattg aagctgcaat
    34141 gcaagctgcc aacgaatcaa acggctactc tgactttgtc ttgatgatta cagatatcgt
    34201 caactcaaac tcagaaatct tggctcttgg tgccaatatg gacaaggtcg aagcggcttt
    34261 caacttcaaa cttgaaaaca atcatgcctt ccttgctggt gccgtttcac gtaagaaaca
    34321 agtggtacct caattgactg aaagttttaa tgcgtaagat tttgggtgtc agctcaaaat
    34381 cggaaagtct agtttgcctt atatcgaaag gagtttcggc tccttttttt ctaggagtga
    34441 agtatgttag aaaatggcga tttgattttt gtgagagatg ggtcagacat gggacaggcc
    34501 atccagactt ccacaggtaa ctatagccat gttgccattt atttggatgg gatgatttat
    34561 catgctagtg gacaggctgg tgttgtctgt caagaaccgg cagacttctt tgagtccaat
    34621 catttgtacg acctctatgt ttacctagaa atggatatcc agtcggtgaa ggaaagagct
    34681 tgcaaacatc ttggagcacc ctacaatgct tctttctatc cagatgcagc tggtttctac
    34741 tgctcccagt atatagcaga aatcctacct atttttgaaa ctattcctat gaaatttgga
    34801 gatggggaac aggagattag tgatttttgg agggagtatt acatagaact aggtctgcct
    34861 gttcctctga accaagctgg gaccaatcct agtcagttgg cagcatcgcc tctgttacaa
    34921 tgtaaagaaa ggaatcttca tgattcagat ttttaatcca tctcgtttga cgagacagcc
    34981 atttttggag aattgatccg ctatctggac cagtatgagg atgtgattct atgtgaaaat
    35041 aagcgctatt acctcaattt tcctatgctt gaatcacttg atagtcttga actggatcaa
    35101 gagatttttg tcagagaagc tagtccggtc tatcaagcct tgttggagca gagttttgag
    35161 acggaattgc gcaatcaaat caatgcagct attttagttg aaaagacgga ctttgcgcgc
    35221 actaaaatga ccctgtccaa ttatttttac aaggtcaaac agcagtatcc tttgacagaa
    35281 aaacagcagg agctctatga cattttagga gatgttaatc ctgagtatgc cctcaagtat
    35341 atgacggctt ttttgttgaa atttctcaaa aaagaccagc ttatgcagaa atgccgtgat
    35401 atctttgtgg acagtttggt tgtcttaggc tatattgtgc aaaatgaaga tggaaagtat
    35461 gagttggcta tcgattttga taaggagaga ttaactttct acttagcgtg atttcttgtt
    35521 tctgagtaca ttgtttgact ttccttagta ttcggtataa actatatgta accggtaaca
    35581 catatcggaa taaactaaag gagacaatca tatgtcactt gaaaacaaat tggaacaagc
    35641 aacaggcgct gtcaaagaag gttttggtaa agttactgga gacagcaaga cagaacttga
    35701 aggagctgtt gaaaaaacag ttgctaaggc aaaagacgtt gtagaagacg caaaaggtgc
    35761 tgtagaaggt ttgaaaaacg tttttactaa ataataggaa aaaatcaagg gtttcatttt
    35821 cccttgattt tttctattct tataaataat tttctgcgac ggctgtatct cctgggtagg
    35881 attctttctt gccctggatg atttggtaac aatcggctcc cttacccgca ataataactg
    35941 catctaattc atgatttgtg atagccattg ccgccttgat ggcttcttgg cgatccgcaa
    36001 tcttttcaac aggatgattg atgtagctac taatttcatc tgcaatggcc attgggtctt
    36061 catagttagg gtcatcagca gtcagaaaga cttgaatctc aggctgttga ttgaggagga
    36121 ggccaaagtc cttacgacga ctttctccct tgtttcctgt tgatcccaga accagagcaa
    36181 tctttccggt ttgatgagtt tccaccacat tgatgagttt tttcagacta tccccattgt
    36241 gggcatagtc gatgaagacc ttggctccat ttttctgagt gaggacttcc atacgaccag
    36301 gaacgcgggt tgcagcgatg ccttttttga tgtcctcaag acttgctccg agacggagac
    36361 aagcaagtcc agcagcaact gcattttctt ggttgaagtt gccaatgagt tggatatcat
    36421 aatctccagc gagtttacct gtagctgaaa agctaaaggc tttggaattc tcgatttggt
    36481 tatcaaattg gctaccatag aaatcatggt cttgatcttc aacctgttct ctcaagactg
    36541 agaagtggtc catgtcactg ttaatgatga ctgctcggct attttccatc aagagacgct
    36601 tgtggtagaa atagtcttca aagctagggt gttcaatcgg gccgatatgg tctgggctga
    36661 tatttaggaa aactcccaca tcaaaggtta gaccatagac acgtttgacc agataggctt
    36721 gactggagac ttccatgatg aggtgggtac ggtcattttg cacagcctga ttcatcatgt
    36781 caaagaggtc aatactctca ggggttgtca acgctgactt aaagaaagtc tcgccatcaa
    36841 gagttgtgtt catggtcgac aacatagcag gtctatgccc ttgagataag atgttatagg
    36901 cgaaataggc tgctgttgtc ttacccttag taccagtaaa ggcaaggagt ttgagttttt
    36961 cctgtggatt accatagaac tccatggcaa tcaaactcat ggctttcttt atatcgttca
    37021 caatgatgac agggataccg acttcgtagt ccttttcagc tacataccaa gctaatcctt
    37081 gtgttatagc agaaagaagg tattcttttt taaaggcagc gccttttgca aaaaaaagag
    37141 tgtcttctgt tacttttcgg ctatcgtagc tgatgctatc aaaaacaact ttgctgtagt
    37201 tgtagtggta atgaccttgg tcaataattt cgcgaaaaag gccatctttc tttaaaatat
    37261 ctaatacggt ttcaatctta atcatacttt ctattgtaaa ccgaaagtcg taaatttaca
    37321 agtaacaagg aaaagtttat aatggaagat aaggagtttt tcctagctat caaaattgaa
    37381 tgaggaatct atgtcgcacg aaaacaatca ccagcaggcc cagatgttac gggggactgc
    37441 ttggctaacg gctagtaact ttatcagtcg cctactcggg gctgtttaca ttatcccttg
    37501 gtacatctgg atgggggctt atgcagctaa ggcaaatggt ctctttacca tgggttacaa
    37561 tatctatgct tggttcttgt tggtttcaac agcggggatt ccagttgcgg tggccaagca
    37621 agttgccaag tataatacca tgcgagaaga agagcatagc tttgccctga ttcggagctt
    37681 cttaggcttt atgacaggac taggcctagt ttttgcttta gtcttgtatg tctttgctcc
    37741 ttggctagca gacttgtctg gcgtgggcaa agacttgatc ccaatcatgc aaagcttggc
    37801 ttggggagtc ttgattttcc cgtctatgag tgttatccga ggatttttcc aagggatgaa
    37861 taacctcaaa ccctatgcca tgagccaaat tgctgagcag gtcattcgtg ttatctggat
    37921 gctcctagca acttttatca ttatgaagct cggttcagga gattatctag cagccgttac
    37981 ccaatcaacc tttgctgcct ttgtcggtat ggtagccagt tttgcagtct tgatttattt
    38041 ccttgcccaa gaaggttcac tcaaaagagt ctttgaaaca ggagataaga ttaacagtaa
    38101 gcgtctcttg gttgatacca ttaaggaagc cattcctttt atcctgacag ggtctgccat
    38161 ccagctcttc cagattttgg atcagctgac ctttatcaat agtatgagct ggtttaccaa
    38221 ctacagcaat gaggacttgg ttgtcatgtt ttcttatttc tcagccaatc ctaataaaat
    38281 cacgatgatt ttgatttctg taggggtttc gattgggagt gttggtttgc cacttttgac
    38341 ggaaaactat gtcaaggggg acttgaaagc agcttctcgt ctcgttcagg acagtctcac
    38401 cctactcttt atgttcttgc taccagcaac ggttggagtg gttatggtag gagaacctct
    38461 atatacagtc ttctatggta agccagatag tttggctctg ggcttatttg tctttgcagt
    38521 tttgcagtct attattttag gcttgtacat ggtcttgtct ccaatgcttc aggccatgtt
    38581 ccgcaaccgc aaggccgttc tctattttat ctatggttct attgccaagc tagtcttgca
    38641 actacctacc atcgccctct tccacagtta tggtcctttg atttcaacaa ccattgctct
    38701 catcattcct aacgtcttga tgtatcggga tatttgtaaa gtaactggtg tcaagcgcaa
    38761 ggtgattttg aagcgaacta ttttaatcag tttgctgacc ctagtcatgt ttctgttaat
    38821 aggaaccatc cagtggctgt taggattttt cttccaacca agtggacgtt tgtggagctt
    38881 cttttatgta gctcttgtcg gtgccatggg gggtggactt tatatggtta tgagtctgcg
    38941 tacctatttg ttagataagg taataggaaa agcccaagca gatcgcctgc gagcaaaatt
    39001 taagctttcg taaggtagca tttataaata aaaggaataa gttgaacaat cttttaggag
    39061 aacttgaaac ttattccttt tttagcgtaa aaatagctaa aattatcttt caaaaataga
    39121 aaatgaggtt tttttgattt tttggaaaaa acaattgact aaacaaacca agagttgtat
    39181 aataaaacaa atatttaaag caggaggttc cggaaatgaa aaagtctaag agcaaatatc
    39241 taaccttggc aggtcttgtc ctgggtacag gagttttatt gagcgcgtgt ggaaattcta
    39301 gcacggcgtc aaaaacctac aactatgttt attcaagtga tccatctagc ttgaactatc
    39361 tagcagaaaa ccgcgcagca acatccgata ttgttgcaaa tttggtagac gggttattag
    39421 aaaatgacca atatgggaat attattccat cattagcaga ggattggact gtttctcagg
    39481 acggtttgac ctatacctac aaacttcgta aggatgccaa gtggtttact tctgagggag
    39541 aagaatatgc gcctgtaact gcccaggatt ttgtgacagg tttgcaatat gcagctgata
    39601 aaaaatcaga agccttgtat ctagtgcagg actctgttgc tggtttggat gactatatca
    39661 ctggtaaaac aagcgacttt tcaactgtcg gtgtcaaggc acttgatgac caaacggttc
    39721 aatatacttt ggttaaacca gaactttact ggaattcaaa aacacttgca acgatacttt
    39781 ttcctgttaa tgcagatttc ctgaaatcaa aaggggatga ttttgggaag gcggatccat
    39841 ctagtatttt gtacaatgga cctttcttga tgaaagcact tgtctcaaaa tctgctattg
    39901 aatataagaa aaaccctaat tactgggatg ctaagaatgt ctttgtagac gatgtgaaat
    39961 tgacctacta tgatggtagc gaccaagaat cactggaacg taattttaca gctggtgctt
    40021 atactacggc tcgtcttttt cctaacagct ccagctatga agggattaaa gaaaaataca
    40081 aaaacaatat catctatagt atgcaaaatt caacttcata tttctttaat tttaacctag
    40141 ataggaagtc ttacaattat acttctaaaa caagtgacat tgaaaagaaa tcgactcagg
    40201 aagcagttct caataaaaac ttccgtcagg ctatcaattt tgcttttgac agaacatctt
    40261 atggggctca gtctgaaggg aaagaaggtg caacaaagat tttgcgtaac ctagtggttc
    40321 ctccaaactt tgtcagtatc aagggaaaag actttggtga agttgtagcc tctaagatgg
    40381 tcaactatgg taaggaatgg caaggtatca actttgcgga tggtcaagac ccttactaca
    40441 atcctgagaa agccaaggct aagtttgcgg aagctaagaa agaactcgaa gcaaagggtg
    40501 ttcaattccc aatccacttg gataagactg tggaagtaac agataaagta ggcatacaag
    40561 gagttagttc tatcaaacaa tcaattgaat ctgttttagg ttctgataat gtagtgattg
    40621 acattcagca attaacatca gatgagtttg acagttcagg ctactttgct caaacagctg
    40681 ctcagaaaga ctatgattta tatcatggcg gttggggacc tgattatcaa gacccgtcaa
    40741 cctatctcga tatttttaat actaatagtg gaggagttct acaaaatctt ggactagagc
    40801 ctggagaagc caatgacaag gctaaggaag ttggactgga tgtctatact caaatgttgg
    40861 aagaagctaa taaagagcaa gatccagcca aacgttatga gaaatatgct gatattcaag
    40921 cttggttgat tgatagttct ttagttcttc caagtgtttc gcgtggggga acaccatcat
    40981 tgagaagaac cgtaccattt gctgctgcct atggtttaac cggtacaaaa ggggttgaat
    41041 catataaata cctcaaagta caagataaga ttgtcacaac agacgaatat gcaaaagcca
    41101 gagaaaaatg gttgaaagaa aaagaagaat ccaataaaaa agcccaagaa gaattggcaa
    41161 aacatgtcaa ataaaatcta attgtagata agtttgtgta agaagtcttt gtgcttaaaa
    41221 acgcataata tcaggtatat aaaatcctag atgttatgcg ttttgttata ggaagattta
    41281 ttagatttta tcctcaaatt gagttattcc cgactttatc cgttgaaata ttataaggag
    41341 tatttatgaa attaaaatta ttaagagtag atactaaggt gattatgggg agtttcttcc
    41401 ttgttctgtc tagtctactt gctttgttgc ttccccttat cttaaaaggt ttaatagatg
    41461 ggagttctat tgaaaatata ggttccaaag tatttcaatc gtttttgatt tttattggtc
    41521 aagccttgtt ttcttctatt ggttactatc tgtttagtca atcgggtgaa aaaaagatag
    41581 caaaaatcag gaaaaaagtg atagaggggt tgatttatgc agagaaatcc ttctttgata
    41641 agagccaaag tggggagttg acttctgcca ttgtcaatga cacgagtgtc attcgtgagt
    41701 ttttaattac gactttccca aatattattc tgagtttagt tatggtactt ggttccattg
    41761 tagtcttatt tagtcttgat tggaatcttt ctctactttt attcatcact cttccttgta
    41821 tgatgtttat tatcttgccc ctttccaata tcagtgaaaa gtatagtcgt cgtttacagg
    41881 aggaaatcgg atttttaaca gggcaattga ctgaaaagat tcaagaacat gaattaatta
    41941 agactaacca agcagagaaa agtgtacagg atgttttgga taattgtatt gaaagggtac
    42001 aaaacaattc actgaaatca gatagggtta cttcttttga gaccccgttt gctctcttat
    42061 ttatctttgc gactatagtt gtgatgctaa cctatggggg ttatcgggtc agcgcaggat
    42121 atatatctgt gggaaccttg gtttcgtttt tgatttacct ctttcaatta cttaatccta
    42181 ttagtaatat agctaatttt gtaactattt attctagtag caagggatct tcagttgcac
    42241 tggagaactt gcttgcagtt cctaaagaaa aatttgaggg aggaaaatcg gtatcaggac
    42301 aagggttgaa ttttaaccat gtctattttg gttatgatga aaatcgacct gtcttaaagg
    42361 atattacttg ttcaattttc aaggggcaaa aaattgcttt tgttgggcca tctggatcag
    42421 gaaaatcaac gattgtgcgt ttgttagagc ggttttataa accgctttca ggagatattc
    42481 taatggagca atcaagtata tatgatttta acttaaaaga atggagaagt aaaatcgctt
    42541 gggtttcaca aaataatgca gtcttatctg gcagtattcg tgacaatctt tgtctcggtt
    42601 tgaatcgctt agtaactgat gatgaattga tgaaagtgct agacttagta tcactaggtg
    42661 atgagattcg ctccatgaaa gagggactag atactgaagt tggtgaacgc ggacgactct
    42721 tgtcaggggg gcgaagccaa agacttcaaa tagctagagc ctacttaaaa gatgctgaaa
    42781 ttcttatatt tgatgaagcc actgctaatc ttgatgcgga ttctgagtat gcgattatca
    42841 gtagcctcta ttctgtacta aaggagaaga cggttgtgat tatagcgcat cgtttgtcaa
    42901 cggtaaaaga tgtggattgt attttcttct tagaggaggg gaaaatcact ggctcaggaa
    42961 ctcataagga actactggaa aatcatgagc gttatgctcg ttttgtgcag gagcaaatga
    43021 tagagtgaag gtgtcttttg agattcacca ttttatagtc tattaaaggg agcaggaaaa
    43081 actccctttt tatatagttt gaaactataa ctagctcttg aaaagaagaa aatgagttga
    43141 tgaaaataag tggtacaata gttactatag atttggagga attgtatgag caaggaatta
    43201 cacattaaca caattttagc acaagcaggg atcaagtctg atgaggcaac aggtgctttg
    43261 gtgacaccac ttcatttttc aacgacctat cagcatccag agtttggtcg atctactggg
    43321 tttgactata cgcgcactaa aaatccaact cgtagtaagg ctgaggaagt cttggcggct
    43381 attgagtcag cagactatgc cctagcgact agctcaggga tgtcagctat tgtactggcc
    43441 tttagcgtct ttccagtagg aagtaaggtc ttggcagtgc gtgatcttta cggtggttct
    43501 tttcgctggt ttaaccaagt ggagcaggaa ggtcgtttcc attttaccta tgccaataca
    43561 gaagaagagt tgattgccga gttagaaaag gatgtggatg ttctctatat cgaaacccca
    43621 accaatccct tgatgttgga atttgatatc gaaaaactag caaaattggc tcatgctaag
    43681 ggtgccaaag tggtggtgga caataccttc tatagcccta tctaccaacg tccgattgaa
    43741 gatggagcag atatcgttct ccattcagca accaagtatc tagcaggcca caatgatgtc
    43801 ttggctggag tggttgtgac caatagttta gaactatacg agaagctttt ttacaatctc
    43861 aatacaacag gggcagtctt gtctccattt gacagctacc agttgcttcg tggtctcaag
    43921 accttatctc ttcgtatgga gcgctcaaca gctaacgccc aagaagtggt tgcctttttg
    43981 aaggattctc cagcagttaa ggaagttctc tacactggtc gtggaggcat gatttccttt
    44041 aaagtagccg atgaaacacg cattcctcat attttgaaca gtctcaaggt cttctctttt
    44101 gcggaaagtt tgggcggagt ggaaagtctt attacttatc caacgactca aactcatgct
    44161 gatattccag cagaagtacg ccattcttat ggtttgacag atgacctctt gcgtttgtct
    44221 attgggattg aggatgctag agatttgatt gcagatttgc gccaagcctt agaaggataa
    44281 gacaaagatg ggaaaatatg attttacaag cctgcccaac cgtttagggc accataccta
    44341 taaatggaaa gaaacagaaa cggatagtga agttctacca gcttggatag cggatatgga
    44401 ctttgtggtc ttgcctgaaa tccgccaagc cgtgcaaact tacgcagacc aactggttta
    44461 tggttatacc tatgccagtg aagacttaat taaggaagtt caaaagtggg aagctacaca
    44521 atacggttac aactttgaca aagaggctct tgtctttatc gagggtgtgg taccagccat
    44581 ctcaacagct attcaaacct ttacaaaaga aggcgaggcg gttttaatta acacgcctgt
    44641 ctacccaccc tttgctcgca gtgtcaagtt gaataatcgt agattgatta ctaattcctt
    44701 agtggaaaag gatggtctgt ttgagattga ctttgaccaa cttgaaaagg atttggtgga
    44761 agaggaggtt aaactctata ttctttgcaa ccctcacaat cctggtggac gtgtttggga
    44821 aaaagaagtg ttggagaaga ttggccaact ttgccaaaaa cacggtgttt tgttagtttc
    44881 ggatgagatt caccaagatt tgaccctctt tggtcacaaa caccagtctt tcaataccat
    44941 caatcctgcc ttcaaaaatt ttgctatcgt cttgagcagt gccactaaaa catttaatat
    45001 tgctggaaca aaaaattcct atgcagtcat tgaaaatcct aagttgagac tggctttcca
    45061 gaaacgcctg ttggccaata atcagcatga aatttcaggc ttgggttatt tggcgacaga
    45121 agctgcctat cgatacggta aagattggct agaggaactc aagcaagtct ttgaagacca
    45181 catcaattat gtggtggatc tatttggaaa agagactaaa atcaaggtca tgaaaccgca
    45241 aggtacctac ttgatttggc ttgacttttc agcttatgac ctgactgatg aaacattgca
    45301 agagttgttg agaaatgaag ccaaggttat cctcaaccgt ggtttggatt ttggagagga
    45361 aggaagtctc catgcccgca tcaatatagc tatgcccaaa tctctgttgc aagaagtctg
    45421 tcagcggatt gtggctactt ttgccaaatg ttaaaaatcc agccttctag gagaaaagtc
    45481 ttcctagaag gctattttca taggcgaaaa tatggtataa taaaaagata aggtaaaggt
    45541 gaaaatatgg ctaaattgat tccggggaaa attcgtatcg aaggtgttgc cctttatgaa
    45601 actggtaagg ttgatatcat caaggaaaag aacaatcggc tctacgctcg cgttgcagaa
    45661 gaagaactgc gctatagttt agaggatgat ttgatttttt gtgcctgtga tttttttcaa
    45721 aagaggggct actgtgtgca tttggcagcg ctagagcatt ttctgaaaaa tgatgagcgt
    45781 ggtcaggaaa tcttgtggag tctggaagaa ggtcatgaag aaaaagaggc tgttgaaacc
    45841 aaggtgacct tgggtggcaa gtttttggat cgaattttat ctccaaaatc agaatgcgcc
    45901 tatgagttat cagctgtggg tcaggtggaa gcaggaacca atcatatctt gtggactctg
    45961 agaatcggcc aaatcaatag ccaaaaatac tatgtcattc gggatattcc acttttttta
    46021 aggattgtcg agcagagaaa gtcgtatatg attggcaaga tttatgagga gtctctttct
    46081 tgggaagcct ttgatgatgc cagccaagaa tttttgactt tcttgcacgg actaacggag
    46141 gaagggctgg ttccagacct ctttttccaa aaccaaggtc ggcacctctt tttccctctg
    46201 accttttttg agcagggtgt gaatttactg atggccttgc cccattttca atttgaccat
    46261 caagtggata gctaccaaac cctgcttttt caggatatgc atgctgatgc caatctattt
    46321 gcctttacag taacagaata ctcggactat tttgaaatgg aaatcagtga gagtcctagg
    46381 gtcaatgtct tttatcaggg agtagtactt ttccataaag gtcaggttta ttttttaaca
    46441 gaccagcaga ttcgtcttct caaggaaatt aaagcgttac ctgtggatcg acaaggaaag
    46501 aaatacctgc aatttgatag cagtgatcga gacaaattgg cctcctgtct gactcttttt
    46561 atccagatgg gcaccgtttc agctccagaa cgtctacaaa tcaaaacttt tgcgccctct
    46621 ttttactttg acagggaaga agataatcgg attcgtttag aaattcagtt tgattatgga
    46681 aatagacagg tatctagccg acaagagcta gaagaattac cattttcaag tgacgtggac
    46741 ttagaagaaa gaattttcca agtctgtttg gctgcgggct ttgaagccga ttttcaatct
    46801 tggcgtcagg ccttaaaagc ggagtctgtc tatcattttt ttcatgatat cattccagtc
    46861 tttgaaaaac tcgggcatgt tgacctatca gataaactgg aagagcttta tagcctagcg
    46921 agtcctcagg tgcaaattgc ctccaaggga ggtctcttgg aaatccagtt tgattttcaa
    46981 gatattgccc aggaggaaat cgaccaagcc atgcaggcct tggttgccaa tcaggatttt
    47041 tatattgatt cgtctaatca agtttacttt ttcgatgaag aaaccaagaa aattcggcaa
    47101 aatctacagg aactggggca atttgaatta aaagatggga ccttgcaggc tcggaaatcc
    47161 ttggcctaca gtttagctca tctctttgaa gggcgagacc gtgtttcttt ttcacaagaa
    47221 ttccagaatc tggcccagga tttgacgcat ccagaggact ttcctttgca agcaactcag
    47281 gtcaaggctg acttgcgaga ttatcaggaa aagggaattg gctggttgca gatgctccat
    47341 cattatggtt ttggtgggat tttggctgat gatatgggac ttgggaaaac ccttcagacc
    47401 attgcttttt tgactagtca agtgacaaaa gaaagtcgag tcttgattct cgctccgtcg
    47461 ggtttgattt acaactgggc agatgagttt cagaaatttg ctccacagtt ggatgtgact
    47521 gttgttcatg gtttgaaagc aagtcgtgag gagattcttg ccgagagcca tcaaatctat
    47581 gtgactagct atgccacctt ccgtcaggac agtgagtttt atcaggggat ggcctttgac
    47641 ttccttttct tagatgaggc tcaggtcatg aaaaatgccc agaccaagat tgcccagacc
    47701 ttgagacaat ttgtggtgcc gtcggtcttt gccttgtcag gaactccgat tgaaaaccat
    47761 ctgggtgagt tgtggtctat tttccaaatc gtcatgcccg gacttttgcc aagcaagaaa
    47821 gaatttatga aattgccagc agagcgagtg gctcagttta tcaagccttt cgtgatgcga
    47881 cgcaagaaag aagaagttct gactgaattg ccagacttga ttgaggtggt ttataaaaat
    47941 gaactggaag accagcaaaa ggctatttac cttgcccagt tacaacagat gcgagaccat
    48001 ctggctcaag tgtcagaaca ggaatttcag cgaagtcgtg tggaaatttt atctggtttg
    48061 atgcgcttgc gtcaaatatg tgacactcct gccctgttta tggaagatta tcagggagcc
    48121 agcggtaaac tagatagtct ccgagaccta ctgctacagg tggcagacgg cggacaccgt
    48181 gtcttgattt tctctcagtt caagggaatg ttggaaaaaa ttgaacaaga actaccagac
    48241 ttgggcctga cttcctttaa aattacgggt tcaaccccat ccaaggaaag acaagacatg
    48301 accaaggcct ttaaccaagg agaaagagat gcctttctga tttccctcaa ggctggtggg
    48361 gtcggtctga acctgacagg tgcagataca gtgatcttgg ttgacctctg gtggaatcct
    48421 gcggtggaag cgcaagccat tggccgtgcc catcggatgg gtcaggaaga aacggttgag
    48481 gtctatcgct tggtgaccaa gggaaccatt gaagaaaaaa ttcaggaact ccaagaacaa
    48541 aagaaacatc tggtgtcaca agtattggac ggcacagagt cacgtggtag tctgacccta
    48601 gcagaaatta gagaaatttt gggaatttct gaagccagca cttgaaaaat caagacaata
    48661 cgctataatg aagtattgaa aggaaataga aaatgaaaca agaacgattt ccattggtgt
    48721 cagatgacga ggtcatattg actgaaatgc cagtcatgaa tctctatgat gagtctgatc
    48781 tgatcagtaa tatcaagggt gagtatcgag ataaaaatta tttagaatgg gctcctattg
    48841 ctgaagaaaa accagtaaaa ccgattgaaa agcaagtagg gaaacctaaa aaagctcctt
    48901 taggggttaa aaaagaagga aagagctatg cggaggtggc gcgtgaagaa gcgcgtgcag
    48961 acttgaaaaa gaaacgctct gctaactatc taactcagga tttcagcctt gcgagacgtc
    49021 attctcagcc cagtctagtt agacagggca atcaaccgac aactcctttc caaaaggaaa
    49081 atcctggtga atttgtcaaa tatagccaaa aattgaccca gtctcattat atcttggcgg
    49141 aagaagttca ttctatccct accaagaatg aagaagtgtc agcacctgct ccaaagaaaa
    49201 acaattatga ttttctaaag aagagccaaa tctacaataa aaaaagtaaa caaacagaac
    49261 aagaacgtcg ggttgcccaa gagttgaatc tgaccagaat gacagaatag gggagaaaac
    49321 atgtcaaaga catatcattt tatcggaatt aagggatcag ggatgagtgc cttggccttg
    49381 atgttgcacc agatggggca caaggttcag ggatcagatg ttgaaaagta ctactttacc
    49441 caacgcggtc ttgagcaggc aggaattacc attcttcctt ttgatgaaaa gaatctagac
    49501 ggtgatatgg aaattatcgc tggaaatgcc tttcgtccag ataacaacgt cgaaattgcc
    49561 tatgcggacc aaaatggtat cagctacaaa cgttaccatg agtttctagg tagctttatg
    49621 cgtgactttg ttagcatggg agtagcagga gcacatggaa aaacttcaac gacaggtatg
    49681 ttgtctcatg tcttgtctca cattacagat accagcttct tgattggaga tgggacaggt
    49741 cgtggttcgg ctaatgccaa atattttgtc tttgaatctg acgaatatga gcgtcacttc
    49801 atgccttacc acccagaata ctctattatc accaacattg actttgacca tccagattat
    49861 ttcacaagtc tcgaggatgt tttcaatgcc tttaacgact atgccaaaca aattaccaag
    49921 ggtctttttg tctatggtga agatgctgaa ttgcgtaaga ttacgtctga tgcaccaatt
    49981 tattattatg gttttgaagc tgaaggcaat gactttgtag ctagtgatct tcttcgttca
    50041 acaactggtt caaccttcac cgttcatttc cgtggacaaa acttggggca attccacatt
    50101 ccaacctttg gtcgtcacaa tatcatgaat gcgacagccg ttattggtct tctttacaca
    50161 gcaggatttg atttgaactt ggtgcgtgag cacttgaaaa catttgccgg tgttaaacgt
    50221 cgtttcactg agaaaattgt caatgataca gtgattattg atgactttgc ccatcatcca
    50281 acagaaatta ttgcgacctt ggatgcggct cgtcagaaat acccaagcaa ggaaattgta
    50341 gcagtctttc aaccgcatac ctttacaaga accattgcct tgttggacga ctttgcccat
    50401 gctttaaacc aagcagatgc tgtttatcta gcgcaaattt atggctcggc tcgtgaagta
    50461 gatcatggtg acgttaaggt agaagaccta gccaacaaaa tcaacaaaaa acaccaagtg
    50521 attactgttg aaaatgtttc tccgctccta gaccatgaca atgctgttta cgtctttatg
    50581 ggagcaggag acatccaaac ctatgaatac tcatttgagc gtctcttgtc taacttgaca
    50641 agcaatgttc aataggatgt tcccatggaa attccaatta agatcattca ggcaagcaag
    50701 tctgatttgc ctgagatagg ggcacttcag acctcgtctt ttccagctga aaagcagcaa
    50761 ctttcccata ttttagaaga aagtatccgt aagtgtgcgg atacctttct tctagctagg
    50821 gatgaaaatc aacttttagg ctatatttta tcaagtcccc agtcagacaa tccgcaatgt
    50881 ctaaaagtac attctttagt catcgagtct gactatcaga gacagggcct gggaacactt
    50941 cttcttgcag ccttgaaaga ggtggcagtt gagctggatt acaaagggat tcgtttggag
    51001 agtcctgatg agctgctttc ctattttgaa atgaacggtt ttgttgatga agaagaaact
    51061 ttgctctatg caactagcca gggctatagt atgatttggt ttaatccctt ttatctggag
    51121 gaacaatgaa aatcagacaa gcaagattag aagatttgga tcggattgtt gaacttgaat
    51181 ttgaaaattt ttcggtcgaa gaagccattc ctccttctgt ctttgaagca catttgagag
    51241 aaattcagac ctcttttctg gttgctgaaa aagaaggaag aatcatgggt tatatcgaag
    51301 gaccagttgg cctgcatcgc catctgcaag accagtcttt tacagaagaa ataaaagact
    51361 atagtcatga gcctggtggt tatatctctg tgacctgtct gtctattgcc aaggaggcac
    51421 agggactcgg actgggtcag aaattgctga cagccttgaa agaagttgct cttgaagatg
    51481 agagagatgg cattaatcta acctgtcatg actatctcat cgcctattat gaaaaacatg
    51541 gatttgtcaa cgaaggccag tcccagtcaa cctttgcagg ggaaacatgg tatgatatgg
    51601 tctgggaaat gaaaaaataa gttaggaaaa gtatcataga ttgctttttt tggactttta
    51661 agatataata ttatggatta tcaacaagga ggaaaaactt ttgagtgaaa agtcaagaga
    51721 agaagagaaa ttaagcttta aagagcagat tctgagagat ttagaaaaag taaaaggcta
    51781 tgatgaagtt ctgaaagaag atgaggcagt agttcgcact cctgcaaatg aaccttcagc
    51841 tgaagaactc atggctgatt ccttgtcaac ggtagaggag attatgagaa aagctcctac
    51901 cgtgcctact cacccaagtc aaggtgtacc agcttctcca gcagatgaga ttcaaagaga
    51961 aactcctggt gttccaagtc atccaagtca agatgtacct tcttctccag cggaagaaag
    52021 tggatcaaga ccaggtccag gtcctgttag acctaagaaa cttgaaagag aatacaatga
    52081 aaccccaaca agggtagctg tttcctatac gacggcagag aaaaaagcag aacaagcagg
    52141 tccagaaaca cctacgcctg ctacagaaac agtggatatc atcagcgata catcacgtcg
    52201 tagccgtaga gaaggagcaa aacccgttaa gcctaagaaa gagaagaagt cacatgtgaa
    52261 agcttttgtg atttcattcc ttgtattcct tgccttgctc tcagcaggtg gttactttgg
    52321 ttaccagtac gtgctggatt ctttgttacc tatcgatgct aattctaaga aatatgtgac
    52381 ggttggaatt ccagaaggtt caaacgttca agaaatcggt acgacgcttg aaaaagctgg
    52441 tttggtaaag catggtctga tttttagttt ttatgccaag tataaaaatt ataccgactt
    52501 gaaagcaggt tactacaatt tgcaaaagag tatgagtaca gaagacttac tcaaagagtt
    52561 gcaaaaaggt ggaacagatg aaccacaaga acctgtactt gcgactttga caattccaga
    52621 aggttatacc ttggatcaga ttgctcaagc tgtgggtcaa ttgcaaggtg acttcaaaga
    52681 gtctttgaca gcggagactt tcttggctaa agttcaagat gagacgttta tcagtcaagc
    52741 agtagcgaaa tatcctactt tactggaaag tttgcctgta aaagacagcg gtgcgcgtta
    52801 tcgtttggaa ggataccttt tcccagctac atactctatc aaggaaagca caactattga
    52861 gagcttgatt gatgagatgt tagctgctat ggataagaac ctatctcctt actatagtac
    52921 tatcaaatct aaaaacttga ctgtcaatga gttgttgacc attgcttcct tggtcgaaaa
    52981 agaaggtgcc aagacagaag atcgtaagct cattgcaggt gtattctaca atcgtttgaa
    53041 tcgtgatatg ccacttcaaa gtaatattgc aatcttgtat gcccaaggaa aactggggca
    53101 aaatatcagt ctagctgagg atgttgcgat tgataccaac attgattcac cttataatgt
    53161 ttataaaaat gtaggtctca tgcctggtcc agtcgatagt ccaagtctgg atgcgattga
    53221 gtcaagcatc aatcaaacta agagcgataa cctctacttt gtagcagatg tcacagaagg
    53281 caaggtctac tatgctaaca atcaagaaga ccacgaccgc aatgtcgctg aacatgtcaa
    53341 cagcaaatta aactaaacaa actaaaatta tgtgatactt cacataattt tctttagaaa
    53401 atattatcag aagaaagttg agaaaaatgg cagaaaaaac atatcctatg acccttgagg
    53461 aaaaggaaaa acttgaaaaa gaattagaag aattgaaatt ggttcgtcga ccagaagtgg
    53521 tagaacgcat taagattgcc cgttcatacg gtgacctttc agaaaacagt gagtacgaag
    53581 cagctaagga tgaacaagcc tttgtcgaag gacaaatctc tagcttagaa acaaaaatcc
    53641 gctatgctga aatcgtcaat agcgacgcag ttgcccagga cgaagtagcg attggtaaaa
    53701 cagtcaccat ccaagaaatt ggtgaggacg aagaagaagt ttatattatc gtaggttcag
    53761 ctggtgcgga tgcctttgca ggtaaggttt caaatgaaag cccaattggg caggccttga
    53821 ttggcaagaa aacaggtgat acagcaacca ttgaaacgcc tgttggtagc tatgatgtaa
    53881 aaatcttgaa ggttgaaaaa acagcctaaa aacagaaaaa ggagtgggga ggcgatgtgc
    53941 ttcactcact cctttttcca ttttgctatt cttcgaaaat ctcttcaaac cacgtcagcg
    54001 tcgccttgcc gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaaccat
    54061 gctttgagct gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg
    54121 cggctagttt cctagtttgc tctttgattt ttattgagta ttagatgtat agtgcattga
    54181 agttaaaata gtacactttg atttctaaga cattgttaga aattagatta aattccttaa
    54241 tcgatttgcg catgttttat ttcatttcac tatactttag attggaaaat aaggaatcac
    54301 ataaatcgca agaaataggg aatctgattc gtgtttataa ccgttcaaaa agagaagagg
    54361 ctgaaagtga gccacttaat ctttatgtcg aagatgaaaa gggcaatctc ctggcaggtt
    54421 tgatagcaga gacttttgga aattggctag aaatcgagta tttgtttgta aaagaggaac
    54481 tgcgagggca aggaatcggt tcaaaactat tgcaacaggc agaaagtgaa gctaagaatc
    54541 gaaattgttg ttttgccttt gtcaatactt accagttcca ggcaccggac ttttatcaga
    54601 aacatggcta caaggaagtt ttttctttgc aagactatct ctacattagg caaagatatt
    54661 attaccaaaa gaatctgtaa ggtgagtatg gaggtataat acaaagagag gctcgcacat
    54721 aagtatggag gtggactgat gtatatgagt aactatcgat aaataccagt tataacctag
    54781 aaattgtgtc tttctagacg atattgagga caatataatc gtagccccaa aattaggaat
    54841 caagacttat caggttaaga aaagaagtga tgttgttgat attttaaaca atttatttaa
    54901 actaaaaact ctctatctat taatccgaga ttcccctctc ggattttgtg ttgctttcat
    54961 taaatttaca ggtaagactt gctagcttca gtaaatatac acaagttttt cagaatagag
    55021 tgaaatccaa aagaaaatgg aaataatgaa agctcttgta ggcataagaa aacacctctg
    55081 tgttatactt gttgttcaac cacaaacaca agaaaggcac agaaatgcaa gacaattata
    55141 ctacaaaagg taaacatttg acaatcgata gccgtcgctt aatcgaaaga tggaaaaaag
    55201 aaggaaaatc aaatagagaa
//
LOCUS       DAVDWM010000010        54749 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVDWM010000010 DAVDWM010000000
VERSION     DAVDWM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.010"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(217..1779)
                     /gene="guaA"
                     /locus_tag="VUW49_000718"
     CDS             complement(217..1779)
                     /gene="guaA"
                     /locus_tag="VUW49_000718"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7980675.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1921..2619
                     /locus_tag="VUW49_000719"
     CDS             1921..2619
                     /locus_tag="VUW49_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7980676.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2662..3558)
                     /locus_tag="VUW49_000720"
     CDS             complement(2662..3558)
                     /locus_tag="VUW49_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7980677.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGILQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLRDLFLEK"
     gene            complement(3567..4502)
                     /locus_tag="VUW49_000721"
     CDS             complement(3567..4502)
                     /locus_tag="VUW49_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET7980678.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4499..5224)
                     /locus_tag="VUW49_000722"
     CDS             complement(4499..5224)
                     /locus_tag="VUW49_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET7980679.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5308..5943)
                     /locus_tag="VUW49_000723"
     CDS             complement(5308..5943)
                     /locus_tag="VUW49_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET7980680.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5945..6772)
                     /locus_tag="VUW49_000724"
     CDS             complement(5945..6772)
                     /locus_tag="VUW49_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7980681.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINTLFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6794..8111)
                     /locus_tag="VUW49_000725"
                     /pseudo
     CDS             complement(6794..8111)
                     /locus_tag="VUW49_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189734.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8359..8970
                     /locus_tag="VUW49_000726"
     CDS             8359..8970
                     /locus_tag="VUW49_000726"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7980682.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9015..9743
                     /gene="rlmB"
                     /locus_tag="VUW49_000727"
     CDS             9015..9743
                     /gene="rlmB"
                     /locus_tag="VUW49_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7980683.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9782..10693)
                     /gene="trxB"
                     /locus_tag="VUW49_000728"
     CDS             complement(9782..10693)
                     /gene="trxB"
                     /locus_tag="VUW49_000728"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7980684.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELSEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLTPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10759..10983)
                     /locus_tag="VUW49_000729"
     CDS             complement(10759..10983)
                     /locus_tag="VUW49_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET7980685.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11061..11804)
                     /locus_tag="VUW49_000730"
     CDS             complement(11061..11804)
                     /locus_tag="VUW49_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7980686.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11804..12604)
                     /locus_tag="VUW49_000731"
     CDS             complement(11804..12604)
                     /locus_tag="VUW49_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7980687.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12762..13118)
                     /locus_tag="VUW49_000732"
     CDS             complement(12762..13118)
                     /locus_tag="VUW49_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET7980688.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13131..13643)
                     /locus_tag="VUW49_000733"
     CDS             complement(13131..13643)
                     /locus_tag="VUW49_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7980689.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13643..14137)
                     /locus_tag="VUW49_000734"
     CDS             complement(13643..14137)
                     /locus_tag="VUW49_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980690.1"
                     /translation="MEIRLAFPNEVGAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKIMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14272..14718
                     /locus_tag="VUW49_000735"
     CDS             14272..14718
                     /locus_tag="VUW49_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET7980691.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTIKLYHQTLDLIDHTQEEVIQ"
     gene            14715..15362
                     /locus_tag="VUW49_000736"
     CDS             14715..15362
                     /locus_tag="VUW49_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET7980692.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15383..15964)
                     /gene="pdxT"
                     /locus_tag="VUW49_000737"
     CDS             complement(15383..15964)
                     /gene="pdxT"
                     /locus_tag="VUW49_000737"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET7980693.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15965..16840)
                     /gene="pdxS"
                     /locus_tag="VUW49_000738"
     CDS             complement(15965..16840)
                     /gene="pdxS"
                     /locus_tag="VUW49_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET7980694.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16992..18371)
                     /locus_tag="VUW49_000739"
     CDS             complement(16992..18371)
                     /locus_tag="VUW49_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7980695.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18665..19588
                     /locus_tag="VUW49_000740"
     CDS             18665..19588
                     /locus_tag="VUW49_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7980696.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19648..20253
                     /locus_tag="VUW49_000741"
     CDS             19648..20253
                     /locus_tag="VUW49_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7980697.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20272..21516
                     /locus_tag="VUW49_000742"
     CDS             20272..21516
                     /locus_tag="VUW49_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7980698.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21739..21996)
                     /locus_tag="VUW49_000743"
     CDS             complement(21739..21996)
                     /locus_tag="VUW49_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET7980699.1"
                     /translation="MDPKKIARINELAKKKKTEGLTSEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22038..24074)
                     /locus_tag="VUW49_000744"
     CDS             complement(22038..24074)
                     /locus_tag="VUW49_000744"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7980700.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24286..25203)
                     /gene="glyQ"
                     /locus_tag="VUW49_000745"
     CDS             complement(24286..25203)
                     /gene="glyQ"
                     /locus_tag="VUW49_000745"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET7980701.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSIYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25571..26332)
                     /locus_tag="VUW49_000746"
     CDS             complement(25571..26332)
                     /locus_tag="VUW49_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7980702.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26605..27447)
                     /locus_tag="VUW49_000747"
     CDS             complement(26605..27447)
                     /locus_tag="VUW49_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7980703.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27561..28952)
                     /locus_tag="VUW49_000748"
     CDS             complement(27561..28952)
                     /locus_tag="VUW49_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7980704.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYSSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29187..29564)
                     /locus_tag="VUW49_000749"
     CDS             complement(29187..29564)
                     /locus_tag="VUW49_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET7980705.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMSYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            29797..30774
                     /locus_tag="VUW49_000750"
     CDS             29797..30774
                     /locus_tag="VUW49_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7980706.1"
                     /translation="MLKLGVIGTGAISHHFIEVAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYSKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGTLFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQDPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30771..31853
                     /locus_tag="VUW49_000751"
     CDS             30771..31853
                     /locus_tag="VUW49_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7980707.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTLIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGSEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31917..32911)
                     /locus_tag="VUW49_000752"
                     /pseudo
     CDS             complement(31917..32911)
                     /locus_tag="VUW49_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(33295..34491)
                     /gene="tuf"
                     /locus_tag="VUW49_000753"
     CDS             complement(33295..34491)
                     /gene="tuf"
                     /locus_tag="VUW49_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7980708.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(35413..36282)
                     /locus_tag="VUW49_000754"
     CDS             complement(35413..36282)
                     /locus_tag="VUW49_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET7980709.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(36506..37126)
                     /locus_tag="VUW49_000755"
     CDS             complement(36506..37126)
                     /locus_tag="VUW49_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7980710.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKKDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKAPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEPTPEPGTPKTETPVNLDPEV
                     PTYETGKREGLSNTGTEANATLASAGIMTLLASLVLGFFKKKEDEK"
     gene            complement(37084..38190)
                     /locus_tag="VUW49_000756"
     CDS             complement(37084..38190)
                     /locus_tag="VUW49_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET7980711.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVIEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(38301..38555)
                     /locus_tag="VUW49_000757"
     CDS             complement(38301..38555)
                     /locus_tag="VUW49_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980712.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(38913..39446)
                     /locus_tag="VUW49_000758"
     CDS             complement(38913..39446)
                     /locus_tag="VUW49_000758"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET7980713.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     RAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(39433..39648)
                     /locus_tag="VUW49_000759"
     CDS             complement(39433..39648)
                     /locus_tag="VUW49_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7980714.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCALPSVWAALKYIKVTLKKDDEL"
     gene            complement(39615..39758)
                     /locus_tag="VUW49_000760"
     CDS             complement(39615..39758)
                     /locus_tag="VUW49_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET7980715.1"
                     /translation="MAYSTDFKQRALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            39992..41710
                     /locus_tag="VUW49_000761"
     CDS             39992..41710
                     /locus_tag="VUW49_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET7980716.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENTALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            41821..42168
                     /locus_tag="VUW49_000762"
     CDS             41821..42168
                     /locus_tag="VUW49_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7980717.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     SGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(42447..43283)
                     /locus_tag="VUW49_000763"
     CDS             complement(42447..43283)
                     /locus_tag="VUW49_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7980718.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(43296..43925)
                     /locus_tag="VUW49_000764"
     CDS             complement(43296..43925)
                     /locus_tag="VUW49_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7980719.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(43935..44576)
                     /locus_tag="VUW49_000765"
     CDS             complement(43935..44576)
                     /locus_tag="VUW49_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7980720.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(44722..45951)
                     /locus_tag="VUW49_000766"
     CDS             complement(44722..45951)
                     /locus_tag="VUW49_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7980721.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(45941..47107)
                     /locus_tag="VUW49_000767"
     CDS             complement(45941..47107)
                     /locus_tag="VUW49_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7980722.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            47251..48264
                     /locus_tag="VUW49_000768"
     CDS             47251..48264
                     /locus_tag="VUW49_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET7980723.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(48309..48728)
                     /locus_tag="VUW49_000769"
     CDS             complement(48309..48728)
                     /locus_tag="VUW49_000769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET7980724.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(48739..50145)
                     /gene="atpD"
                     /locus_tag="VUW49_000770"
     CDS             complement(48739..50145)
                     /gene="atpD"
                     /locus_tag="VUW49_000770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7980725.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(50231..51109)
                     /locus_tag="VUW49_000771"
     CDS             complement(50231..51109)
                     /locus_tag="VUW49_000771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET7980726.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(51125..52630)
                     /locus_tag="VUW49_000772"
     CDS             complement(51125..52630)
                     /locus_tag="VUW49_000772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7980727.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(52645..53181)
                     /locus_tag="VUW49_000773"
     CDS             complement(52645..53181)
                     /locus_tag="VUW49_000773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET7980728.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(53181..53675)
                     /gene="atpF"
                     /locus_tag="VUW49_000774"
     CDS             complement(53181..53675)
                     /gene="atpF"
                     /locus_tag="VUW49_000774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET7980729.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(53689..54405)
                     /gene="atpB"
                     /locus_tag="VUW49_000775"
     CDS             complement(53689..54405)
                     /gene="atpB"
                     /locus_tag="VUW49_000775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET7980730.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(54440..54640)
                     /locus_tag="VUW49_000776"
     CDS             complement(54440..54640)
                     /locus_tag="VUW49_000776"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET7980731.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT        16235 a        12061 c         9927 g        16526 t
ORIGIN      
        1 atattagaaa gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca
       61 gtttgaaaac tacggtctaa ttttttgtcc aaatgtaaac cacttggaac tattattcaa
      121 tttattcttt ttagcaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta
      181 accgttgatt ttacaaagct tttaattttt ttgcgattat tcccactcaa ctgttgcagg
      241 tggtttactt gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg
      301 tactgagatt ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc
      361 gatagaagtg atagcacgga ttgcaatcgt gtagtcatac gtacgaccgt cacccataac
      421 accgactgaa cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc
      481 agctttagcg atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc
      541 ttcagtgatt tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca
      601 tacgatatgg tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag
      661 agtattgagt ggttcaatca attcaaactg catatcttct ggaagaccac ccacgttgtg
      721 gtgtgacttg atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt
      781 accttgagca aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac
      841 aaactcgtta ccgatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc
      901 aaggaaacgt ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat
      961 gtccataact tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat
     1021 caattgatcg ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc
     1081 tgatagacca agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat
     1141 gtcaatgaaa ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt
     1201 acgaaggata tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta
     1261 aatgtgttta tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg
     1321 aacaaagtca gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc
     1381 atcaggtgtt gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc
     1441 acgatttcca gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg
     1501 cataccataa cagattccca aaattggaat tccgagttcg aagatttctg ggtcaatatc
     1561 aaatgaacca tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt
     1621 gacttcacga acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat
     1681 ctcacggata cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat
     1741 tttttctaca tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa
     1801 atatcttttc ccaatattct atcacaaata agggcgaatt accaactaaa caaggcgaag
     1861 tttgactttt tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat
     1921 atgctacctg cttatataaa aatccatgat cagataaaaa aggatattga cgagtaccgt
     1981 tgggctattg gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt
     2041 cgcatgaccc tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt
     2101 gtaggaagcg gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc
     2161 agttttactg aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac
     2221 aaaaaaacca ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat
     2281 attatccgaa tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct
     2341 tctattcctg aaaaattcat taaggacttt aaaaaagaag aaatcaccag tcatttcttc
     2401 caaaccttgc aaaaacatgg ctatcgtatc ggcaaatctc aacagaccat ctatgctcgc
     2461 cttgctaaag aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga
     2521 ttgacccagg tttcctacct agaagatggt actgcttttg aatacgtaaa aagtcaatat
     2581 gtaggcgaac gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct
     2641 gtcacggaca aagagtcttt tttatttttc taagaataaa tctctcaaag aaatcaaagt
     2701 tacagcaaac aatatcattg ccataccaat aaaatcaatg ggataaaatt gttcattcat
     2761 tattaagaag gcaaagaaaa ccgccgatat tggctcaatt gaagccaaca agcttgactt
     2821 gaccggtcct atcagactgg ctcctttaag gaaagctgta taggcaaaga cagtcccgat
     2881 aaggataatg cctgcaaacg caaggagaaa atcaagacta gtcgggatat cggcctgtag
     2941 aatccctgta aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgaccaa
     3001 gctgctcccc cactttttaa tcaaggctat gggtaaaatg atatacaaag cataagtcaa
     3061 ggcagaaaag agaccccaga acagaccagc aggtgtcatg gataactggt ccaactgccc
     3121 atgtgttgcg atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag
     3181 tgtcggtgcc accctatcct tgatacagct ataaattaaa attccgacag gacaaacata
     3241 ctgaagcacc gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg
     3301 gttgagaaaa agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt
     3361 taaaaagacc agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc
     3421 agcgattaaa agacgcaagt tggtcaagac cagagccgaa attccgtgtg ccattaggta
     3481 ttggccactc gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc
     3541 ttttaaacta tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga
     3601 ttgcggtcta atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa
     3661 atagtggctt cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg
     3721 tctagccaat ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa
     3781 aaattttgat ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaagatttgt
     3841 aaatccttta tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc
     3901 ttgggatcat gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact
     3961 ggcccaaact tagtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca
     4021 tccaaatctt gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc
     4081 gaataaagaa aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca
     4141 ggggctgtta aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag
     4201 agctggcgaa tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta
     4261 tcaatatcta attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc
     4321 ttgctgacac tagctaggtc ataaactagt cctgcctcag tctgcaagcc atgctctggg
     4381 gcactctggc ctaaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct
     4441 ccgggataaa ttcctgcctc gatttgttct tctatttttt taataatctt ggtccacttc
     4501 atacttcttc aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag
     4561 acttaggaat aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt
     4621 cattgaccaa gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat
     4681 cctgcccctg agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac
     4741 tttcgatatc agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg
     4801 atgccaaatc tgtttgaaat tcaggctttt ctcctacttc tactagactt gctctgtcat
     4861 cttccgcatg aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat
     4921 tttgaccttt atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta
     4981 agggattttc caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag
     5041 tacgcatact gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg
     5101 acagaaaggc cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt
     5161 catttaattt tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat
     5221 tcataaattc ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta
     5281 tgctcaaatt taacattatt tagctattta gtaaagatag tctttcaagg cttttgacaa
     5341 agcctgataa ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg
     5401 gcctgcttgg tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga
     5461 tgcaagctct tgataggctt gattccagtt ctgaattttt tcattcgagc ggatatagac
     5521 tgcctgctgg tactcctctc cctcattgac aggcaaaatg gaaagcaatt taatctctgt
     5581 caatggataa tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt
     5641 cacaggaaca tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc
     5701 tccaccatat agatgagcat caaggttctc tagtaacagt cctgtctgat agccacgaat
     5761 tcctcgattg acaatcgtct ttgaagtccc aaatagctcc tgtagaggat aatactcgac
     5821 aatggaatcc ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg
     5881 atacttggtt tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc
     5941 cacttcattc tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctgt
     6001 tggctggtgc tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat
     6061 aggcaattcc agccatttca agcatttcaa catcattttc actatcacca aaagccatga
     6121 tttcttggga tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa
     6181 tcccagcttg gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat
     6241 caaagagagt attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc
     6301 tcatcttgag cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt
     6361 ggtagaattt ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac
     6421 cttccttgac aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac
     6481 ccttgaaatg agccaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca
     6541 attcattgtt ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca
     6601 agggactcag tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa
     6661 tgcccctttg atccaactga tctaaaatct tttccaatcg tgggagatca agctgacctc
     6721 tagcatccag caaggtccca tccatatctg tcgcaattac cttaatcatc atttactttc
     6781 ctctggattc aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg
     6841 tatcaattcg ttccttagtc gcttcatcta actggtcacg atgactgcca tcctctataa
     6901 aatcttccaa aatataagtt tggtaatgat acaagggata tccttctggt gaaaaggttc
     6961 cctttggtgt tcgattgacc caagtaggat gatctttagc tgttccgata gttgtttttc
     7021 catcattctt cttgatggtg acatccatga gaacaccacg ttcagtccac ttagcattct
     7081 cttcatctcc catagattca attcgttgat tggaaatgaa gttccccatt gaatagataa
     7141 tgagtttctt atctccatct ttttcaaccg tttcagatgg ttcaacaacg tgaggatgcc
     7201 ctccaaagat aatatccgct ccccaatcga tcatcttgtg ataaagagct ttttgttctt
     7261 cagttggttc caatcgatac tcaacaccca tctgaggcat gataatggtg atatctgctt
     7321 ccttctctgc ccgttcaact tcagccttca tcttatcttc gtttaaatct aaaagataac
     7381 gattatagtc ttcctgagaa atatactgct caattccatt gaaaccatag gaataggctg
     7441 acaatgcaac cttgatacca ttcacttcct taatgaccag cggaacctga tcacgtggtt
     7501 cgtgcgtata aactccgatt ggagtgattc cagctttctc aataatatcg gccgttgaaa
     7561 taactccctc aatttgcgaa tccaaaatat gattatgagc taaatccagc acatgataac
     7621 ctgcctcctt aatagcatcc ataacttcag caggagcatt aaagagagga taacccgcta
     7681 aataatgatc cttattaatg gtttcttcaa aatcaccaat agctaaatct gcttgcttga
     7741 accaaggagt cacatactca aaattttcat gaaagtcata ggtaccgtct tcttttttag
     7801 ctgaaaagaa aagtccatcg tggtagagca aatccccgtg ggccataatt ctggcagttt
     7861 tttcctcttc ctactcctga gacttttgct tagttccctc ttgagaaata gtatctcgtt
     7921 tttgactagt aaagagattc cttggaagct ttcaaacaac aagaccaagc ccattgataa
     7981 accaactgct agcaagagta tcgccacaaa tcccttattg ctccacttgc gataactcct
     8041 aaaaagttta ccaagccctt cataaaacga aaagctaaac caccctgatt tcgattttgt
     8101 cttctttgta tcttcgttct ccctactttc ttatgcaagc cttttctttt tattatatca
     8161 cagataagta tttctttcac aattgaattg aacttcccat ctattttcta taaatcctaa
     8221 atgccataat gctttcaatt cttgtcattt tgtgatatca tgtagaggaa atgaactaat
     8281 ccacagtggc ttattccaag tataccactt gggctttggc agtagctaac tgcactaaac
     8341 ataatataag gagagaaaat gtctgcaata gaacgtatta caaaagctgc tcacttaatt
     8401 gatatgaacg atattatccg cgagggaaat cctactctac gcgcgattgc tgaggaagtc
     8461 actttccccc tatctgacca ggaaatcatc ctaggcgaaa agatgatgca attccttaaa
     8521 cattcccaag atcctgtcat ggctgaaaaa atgggactcc gcggtggtgt tggactggct
     8581 gctccccagt tagatatctc aaaacgcatt atcgctgttt tggtacctaa tattgttgaa
     8641 gaaggcgaaa ctccacagga agcctacgat ttggaagcca ttatgtacaa tccaaaaatc
     8701 gtctctcact ctgttcaaga tgctgctctt ggcgaaggag aaggttgcct gtctgttgac
     8761 cgtaacgtgc ctggctatgt tgttcgccat gcccgcgtta ctgttgacta ctttgacaaa
     8821 gatggagaaa aacaccgtat caaactcaaa ggctacaact ccattgttgt tcagcatgaa
     8881 attgaccaca ttaacggtat catgttttac gatcgcatca atgaaaaaga cccatttgca
     8941 gttaaagatg gtttactgat tcttgaataa agaaaatccc gttgcaagac ggggttttgt
     9001 gttataatag aggcatgaaa acaaatgata ttgtctatgg tgtccacgcc gttaccgaag
     9061 ctctccttgc aaatacagga aacaaactct acctccaaga agatctccga ggtaagaatg
     9121 ttgagaaagt caaggaacta gctacagaaa agaaggtgtc catttcttgg acatcaaaaa
     9181 aatctctctc tgagatgact gaaggtgctg ttcatcaagg ttttgttcta cgagtgtctg
     9241 aatttgccta tagcgagcta gattacatcc ttgcaaaaac acgccaagaa gaaaatccac
     9301 ttctattgat tctagatggt ctaaccgatc cccataatct gggttctatc ttgcgaacag
     9361 ccgatgcgac caatgtttca ggtgtcatca ttcccaagca ccgtgctgtc ggagtaactc
     9421 ctgtcgttgc caaaacagcc acaggtgcta ttgaacacgt tccaattgcc cgagtgacaa
     9481 acctcagtca aaccttagat aaactcaagg atgaaggctt ctggaccttt ggaacggata
     9541 tgaacggtac tccttgccac aagtggaata caaaagggaa aatcgccctc atcattggaa
     9601 atgaaggaaa aggtatctct agcaacatca aaaaacaagt cgatgagatg attaccattc
     9661 cgatgaatgg acatgttcaa agccttaatg ccagtgttgc tgcggccatt ctcatgtacg
     9721 aagttttccg aaatagacta taaaaaagtt tccagtcatc tgattggaaa cttttttatg
     9781 attaactatg ttctgtgata aacttgtagg cttcttgacc agcgatagct ccatctccaa
     9841 ctgctgttgt tacttggcga aggtctttca agcgaacatc tccaactgca aagataccgt
     9901 cgactgcagt tttcatgtgg ttatctgtca caatccatcc tgcctgatct tggatattca
     9961 attctttaac aaaatcgcta agaggggtca aaccaacata gataaagaca ccaccgaagg
    10021 cttgttctgt cacttgacct gttttcacat tttcaaatac gactgattct actcggtttt
    10081 cacccttgat ttcccttact acagaatccc agataaagct gattttttca ttcgcaaagg
    10141 cgcgatcttg taaaaccttt tgggcacgaa gttggtcacg acggtgaaca atggtaacag
    10201 tcttagcaaa acgagtcaag aagagggctt cttcaacagc tgaatctcca ccaccaacta
    10261 ccaataaatc ttggtcacgg aagaaagcac catcacacac agcacagtaa gaaacaccac
    10321 gactgttcag ttcttcttct ccaggcactc ccaaaggacg gtgtttagaa ccagttgcta
    10381 cgataactgt acgtgtttca tatgtttggt catcagtcat cactttctta aaatcaccat
    10441 ggtcttcgac attttcaaca taaccataaa tgtgctcaac accaagattt tcaagtggtt
    10501 caaacatctt ttcagacaat tcaggtccac taatattagc gtatcctggg taattttcga
    10561 tatcagatgt attattcatc tgaccacctg gcagaccacc ttcaatcaaa gctactttta
    10621 gattgcttcg agcagcatac aaggccgcag tcatccctgc aggtccagca ccgataataa
    10681 tagtatcgta catatagatt ccttctttct tgttgtaact atctttattc taactctttc
    10741 ttgtcaatca cgcaaggatt atgccttgaa aacaagaatt aaactcataa ccgcaaagga
    10801 taatactggg acaaccaaga ctccaatcat aatcatatca tagagatggg cttggcgagc
    10861 cctggctgtc ttatcaactc ccgacatggc tctcagacca atccaaatcc ctaaaaaaat
    10921 cagaacaagg atggtggtca agatcaaact ctcgaaatat aaagaaaata gttgcagtag
    10981 catgatttct ctcatttcta tcttttttaa agagtaaact cagctagtcc aactaactga
    11041 gttttccttt atctattata tcaaatataa gtccgtttgt aactagcgaa gaattctttt
    11101 gtccgctctt ctttaggggt gtggataatc tcatccggag ttccagactc gatgattttc
    11161 cccttatcta agaagagaat tttatccgca acttgggcta caaaggacat gtcatgactg
    11221 accaaaatca tggtctgacc tgacttagca gcatctgcaa tagacttttc tacttcaccg
    11281 accaattctg ggtcaagggc tgaagttggt tcgtctaaga gcaaaacatc tggtttcata
    11341 gcaagcgcac gcgctagggc aacccgttgc ttctgtccac ctgataaatg gcgaggataa
    11401 tggttttcac ggtccgaaag cccaacctta gccaactctt ccttggcaat cttagtcgct
    11461 tcttggtcag ataatttctt gacaacaacc aagccttctt tcacattatc aagtgctgtt
    11521 cggcgttcaa acaaattaaa ctgttggaaa accatagaca acttacgacg tagggcaagg
    11581 atttcttctt gagtgatttt agaaaaatca actgaaaaac catcaatctg aatagagcca
    11641 ctgtcaggtg tttcaagata attgagactg cgaagaaagg ttgattttcc agctcctgaa
    11701 gaaccaatca aggctacaac ttcccctttt tgaatatcca agtccagatg atccaagaca
    11761 gtctgtcctg aaaaggattt gcttaaattc gaaatcttaa tcattaacga aggtctcctt
    11821 tcacatctgt ttgcactgta tcaggtgcag aaatagccat ttttctctcg atgaaacgac
    11881 cgaggctttc aattccgata ttgactaccc aataaacaag ggcaacggag atgaagcgtt
    11941 caaaatagcg ataatcagct ccacctagaa tctgagcttg ggcaaagact tccacaacac
    12001 ccgcactaaa agctagagat gttcccttgg tcaaaccgat gagggaatta atcaaggttg
    12061 gagtagctac caccgctgca ttaggaataa tcactcgtcg ataaacttgc gctcgggtca
    12121 tacccagact gcgtgccgcc tcaatctcac caggattaac tgagagaatg gctgcacgaa
    12181 tggtttcact agcataagct gcctcattaa aggcaaaggc gacaatcgca aaagctgcag
    12241 ctggaatcgc attgatattg agaccagttc cccattgctg attgagggct ttcaaagcca
    12301 aagggattcc gtagtaggtc aacatgagtt gcaccaaaat cggtgtccct tttaagaaac
    12361 taacaaagaa ggcctgcaag ggatataaaa tcttgacacg attgatcttc acaatggcaa
    12421 aaagaagcgc caaaaccaag ccaaaaaggg caccaccaat tgtcaacata attgttgttg
    12481 gaagttgttg gacaattcta gggattccat caaagaccga acgtaggcta aacagcttgc
    12541 catccggaat caattgcatc aagttttggt accaatctga tgctaaaatc gttgtaacat
    12601 tcataaaaac atcctcttct gataatgatt cattctacca tgcttctgcc gtcaatgaaa
    12661 ttatttgcta cggtcagata cagactttgt ctacctacta gtttatcata ctttgaaaca
    12721 gggaagttat atattttatc tatcaaagag atagataaaa actatttcag tcccagttct
    12781 tcataagatt ttcgataacc gatttgctta acagttccat tttctactaa aatgggccgt
    12841 tttaacaaca taccgtcact tgctagcaac tcagccgctt cttggtttga caaacttcct
    12901 accttatctt ttagccctaa ttcacggtat ttgataccac tggtgttgaa aaattgcttc
    12961 aattcaaatc ctgaggtttc tagccaattc aaaatgactt cttggctagg tgtttcttcc
    13021 acgatatgga cggctttata gtccacacct aattgattta attcttgttt tgctttttta
    13081 caagttgaac atttggggta ttcgataaat tctaacatgt gcttctcctt ctatttgttt
    13141 atatctttaa aatctacgcc ttcatgctcc aagagccaag ctttcttttc cactcctgca
    13201 gcatagcctg tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac
    13261 caaggattgc gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga
    13321 gcaatttgtc cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact
    13381 ctcttttcaa aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg
    13441 cctttaaagt aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact
    13501 tcttctatcg tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt
    13561 ccatacaaat aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagg
    13621 atttttacat acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat
    13681 ctactggcac cttaccaact tgtttaaaac caagtttttc aaaaatatgt tgcataatct
    13741 tgttttcagc atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct
    13801 ctaagaaggt ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa
    13861 agactagata ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct
    13921 cctctggact cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt
    13981 aggcttgccc tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc
    14041 actggttact accagcatct gctaaacatt ttttggcatc ctctatcacc tgcatgatgg
    14101 cacctacttc atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag
    14161 tttctcccat catagcataa tttaagtctt tctacaagca gttaaaactt gactttgtat
    14221 ttttgttatt ttataatcta gttatcaaaa ctatataaaa aggagttgaa aatgaaaact
    14281 accttttcct acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt
    14341 ttgctctatg tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca
    14401 gcatctatgg tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa
    14461 taccaacgcc aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct
    14521 attcaagaaa tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc
    14581 tacgatgctt ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa
    14641 accagctgcc aaaccattaa actctatcat caaactctag acttaatcga tcatactcaa
    14701 gaggaggtaa tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg
    14761 agattgctaa tagcgtgacc catgctgtag gtgcagtcat catgcttatc ttgctgccta
    14821 tttcatccat ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt
    14881 tcgtcatcag tctctttctc atgttcctat catccaccat ttatcactct atggcctatg
    14941 gttcgaccca caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg
    15001 ccggctcata cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta
    15061 ttgtcatcca atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg
    15121 tcaatgagaa atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca
    15181 ttcctgccat tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac
    15241 tctgttatac agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt
    15301 ggcatctctt tatcctagct gcgtccgcac tccaatacat cgctattgtt tattacatgt
    15361 aaaaaagttg agaagttcaa tctcaacttt tttctttaca catattgata aagtactggt
    15421 gcaagcgcac atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt
    15481 gggctgcaac aatttgattg ttcactgttg ctagaatttc tacaccctca ccaacactac
    15541 tgataatcgg accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt
    15601 ccgtgtagaa acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag
    15661 ttcctagatg actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac
    15721 aggtcccaaa cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct
    15781 ggtcacgtaa gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca
    15841 agtcactctg atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta
    15901 attgatctag cacttttgca tgttctgcaa aggccccttg caaggccaat attccgattt
    15961 tcatctattt tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca
    16021 tggcttctcc taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca
    16081 cagccttaac aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac
    16141 cgacaaagac cccctctgcc cctagttgca tcattaacgc agcatctgct ggcgttgcaa
    16201 cacctccagc agcgaaattt acaactggca attttccatg ttcatgaaca tattggacca
    16261 attctacagg gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt
    16321 gaatgcggcg aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc
    16381 ctgtccctgg ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg
    16441 cttcacccaa atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat
    16501 ggaaacggtc atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag
    16561 cctctaaaat ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac
    16621 taaccgcttc ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag
    16681 ctgcacgaat atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt
    16741 ctgcgatacg agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct
    16801 gtgccaagtt tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt
    16861 gtattggtag ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac
    16921 tataattgta tggggacaaa ttagctaaaa actatctgac cttaacttaa ggccagatag
    16981 ctcattcatt tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggtttg
    17041 ttgaagtgtg gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct
    17101 tggatagcaa gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt
    17161 tgggcaccaa gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg
    17221 ttgtcatgtt tcatgaattc tggtttttga agatcgttaa agcctgtttc agttgcgttg
    17281 taaccagcag ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag
    17341 attgagatac catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcattg
    17401 taggcaccaa cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct
    17461 ttacgagcat tgtcataaac agtcgcacag tcaccaacag cgtaaacgcc tgggatagat
    17521 gtttcttgtt tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccatct
    17581 gcaagggctg tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt
    17641 tctttgtcag taatcaagcg ttcaacttta ccgtcacctt cgattgcttt aacagtttga
    17701 cctagagcca agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct
    17761 ttgtcatagt aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca
    17821 agacgttcaa aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata
    17881 cggtcgaggt gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac
    17941 aatttcacga attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt
    18001 tcaacacctt cgattggtgg caagattggt gtagaacctg tagcgaaaat caatttttcg
    18061 tatgattctt tgtgctcttt tccttcaact tccgctgtaa ctactttgtt atcatagtcg
    18121 attgaaagaa caggtgagtt catgtaaact ttagcacctt tagcttccaa tttttcttta
    18181 tcagaataga acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca
    18241 catcctagga aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca
    18301 aaattatcca acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg
    18361 attttactca taaaaatttc ctgccttcat tctaaattta ccttactagt ataccattta
    18421 ccgttagcgt ttacaagggg ggtactatta tttcagcatt ttttatcaaa aataattccc
    18481 catttatcct gttttcaagc ctcatgaatt ttatgagcat atttcttgac tttttccaaa
    18541 ttttgtttgt gaaaattcta actttatggt attataataa catgaaaatg aaatgtaagg
    18601 gcttcaattt agatgattat ttgatattac aacttatttt ctataagaga aaggagttgg
    18661 tagcttgact cttcgttcac attctgaacg gctaatgggg actactatca ctatttcatt
    18721 agtggatgag caagccgata tctttctcca aaaatccttt gacttgctca aagagcttga
    18781 ataccgcttc aacgccaata gtcaagaatc tgagttgatg gaaatcaatt atcaagcagg
    18841 aatatccccc gtcacggttc atccagacct gtttgagctg atttcacttg ggttagagca
    18901 tagcctagcg ctctctagcc acctcaatat cagcattggt cccttaattc aaacctggcg
    18961 tatcggtttt tcagatgcca aggtcgccca gcctcaagaa attgaatcgg tgctacctct
    19021 aatcaatcct catggtatcg agttagattc ttctacttct actgtgtttt taaaacagaa
    19081 aggaatgaag atcgatctag gttgtttagc caaaggatac agtgcggata aggttgccca
    19141 atttcttagg aaagaggggg tgacttctgc cttgatcaat ctgggaggga atatcctgac
    19201 cattggaaaa aatcaggcaa gaggggataa cccatggcaa atcgggattc aagacccagc
    19261 caatccgagg ggaaatcatt taatgaccat ccctgttgtc aataaatctg tcgtgacttc
    19321 aggcatttat gaacgtcacc tgaccgtcga tggacaagat taccatcaca tttttgacag
    19381 tcaaacagga tatcctgttg aaacggaact agcgagtcta acaatcatct ctgataaatc
    19441 agtcgatggc gaaatctgga cgactcgact ctttggagaa agacctgctt ctatcctctg
    19501 gcaagtcgaa agtttggagg gcatcgaagt catcctcatc gataaagaag gccacctaag
    19561 ctgttcttca ggaattccta ctctatagag acaactgttt caatggaatt ttaaaattgt
    19621 attatgtaaa aagagaaagg aaatctcatg ttaaaactta ttgctattgt tggaacaaat
    19681 tcaaaacgtt ctacaaaccg tcaattgctt caatacatgc aaaaacactt tgctgacaaa
    19741 gctgaaattg agcttgttga aatcaaggcc attcctgtct tcaacaaacc agctgacaag
    19801 caagtacctg ctgaaatatt ggaaattgct gctaaaatcg aagaggcaga tggcgttatt
    19861 atcggtactc ctgagtatga tcactctatt ccagctgttt tgatgagcgc tcttgcttgg
    19921 ttgtcttatg gtatttaccc acttttgaac aaaccaatca tgattacagg tgcttcttac
    19981 ggtacgcttg gttcatctcg tgcccaattg caacttcgtc aaatcttgaa tgctcctgaa
    20041 atcaaggcaa atgttcttcc agatgaattc ttgctctcac actctcttca agcatttaac
    20101 ccaagtggcg acttggttga ccttgatgtt atcaagaaat tggatgccat ctttgatgac
    20161 ttccgtatct ttgtaaaaat cacagaaaaa ttacgtaatg cacaagaatt acttcgcaaa
    20221 gatgctgaag actttgactg ggaaaatttg taagatagga gaccaaaaag aatgaaattt
    20281 gttggacttg ttggatcaaa ctacgatcaa tcatataacc gtaagctctt ggaatttatc
    20341 cgtcgctact tcaaactcaa atttgaatta gaagttcttg aaattgacga agttccaatg
    20401 tttaaccaag atgaaaaatg ggatgaaagt ttccaattac gtttcttgta taacaagatt
    20461 acacgtgcag atggtgttat cattgctact cctgagcata accacactat ctcagcttca
    20521 ctcaaatctg tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt
    20581 atgattgtgg gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt
    20641 aaaatccttg atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt
    20701 ggtaaagcta aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa
    20761 ttccttgaaa cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa
    20821 aaaccaaaac caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc
    20881 acagaagttg accctgacga tccagaatgg gtagaaaaag ttgcagcaat cactggtgct
    20941 gtttctggtg atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat
    21001 atgttcttga aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc
    21061 tactacaaca acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa
    21121 tcaggtagcc gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta
    21181 gagtgggtta tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca
    21241 ggttctcctg caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga
    21301 tcatacgctg gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta
    21361 aaagagactg gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt
    21421 catggagacg ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt
    21481 ggtgacgcag acgctacagc cggtgcaagt tactaatgat agtttttagg aaccattatg
    21541 gttccttttt tagcgaaaaa agactagcaa acacaaactg ctagtctctt tgttatcgtt
    21601 tcatctatct ttgttaaaag aaagaataca tatactcaat gaaaatcaaa gagcaaacta
    21661 ggaagctagc cgcaggctgt acttaagtac ggcaaggtga agctgacgtg gtttgaattt
    21721 gattttcgaa gagtattatt atgaatttgg atcatcaaga ctacggccat gtaatccttt
    21781 ttcacgttgt acttggcgta gtttttctgg tgtaacatcg tttccttctt cgtccacaat
    21841 tttgattcct tcaatgtggt gacgaacagc gcggcgataa ccttcgatgt actcctcacg
    21901 tagtttggct tgttccactt tttcttctga tgttaagcct tctgtttttt tctttttagc
    21961 aagctcattg atacgagcaa tttttttcgg atccatttct tctcctttgt aataagataa
    22021 agtctgttta tcaaatttta tttagtgtta atttggttaa aacaagcaaa cttagctgct
    22081 ttcttggtta gttgtgacaa gattgccaaa cgattttgac ggacagcctg atcttcagcc
    22141 attaccatag tattttcaaa gaaagcatca atgactgggc taagcgcaaa gagttgtttc
    22201 aattgctgac ttgcaggtcc tgataaaacg agtgtttcta ctgcttctgc caaagctttt
    22261 tcttggtcat tctcaaatag tgctgaatca actgtagcaa ccccttctgc cttctcagcc
    22321 aggttaaagg cacgagaaag tgattcaaca gatggtttaa aatcttcttc cttgcttact
    22381 tctacgagag cacttgctgc ttccaacata tctgccacaa caaagtttga acctgcaaga
    22441 actgcttctt tgatatcttt tggagtagag cccatcatct tatcaacacg agccttgata
    22501 aagtccataa cctctgcttt attttcataa gtcaaactgt caaatttcaa tgcataaagg
    22561 ctatcaatca gctcatccat agcaatgtgc caaccaaagg catccaagat acgaaccaca
    22621 ccttgagttg cacgacgaag ggcataaggg tcattagaac ctgatggaat caatcctact
    22681 gagaagaaac tcaaaatcgt atccaatttg tctgcaatgg ctagaactgc gccgaccttg
    22741 ctctctggaa gttctccttc agctgatgta ggcatgtagt gttcacgaat agcagctgcc
    22801 accgctggag tttcaccagc aagaagggtg tatttttcac ccataattcc ttggagttcg
    22861 tcaaattcac caaccatacc tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca
    22921 aggtcaactg tttcatccac tgacaaacca gctttttctg ccaaaagtac agtgatttga
    22981 cccgtacgaa tcatgtgttc acgaagggaa ccaatcttct catggaaggt gacattgttt
    23041 aatttttcaa caagatctga aatcaccaat ttttggtctt cacgccagaa gaattctccg
    23101 tcttccaagc gggctaccaa gactttttca tttcctttga tgacattttt caaacgctct
    23161 gcgtttccgt tacgaacaga aatgaagttt ggcaagagtt ttccatcttg atcacgaaca
    23221 acaaagtaac gctggtgttc cttcatagaa gtcaccaaga cttcttctgg aacttcaagg
    23281 tatttagcat caaaacttcc catgaaggca gttgggtatt caaccaaatt caagacttca
    23341 ttcagcaaat ccgcatcaat ttcgatacgt acaccatgtt ttgcctcaat ttccttgatt
    23401 tggtcaacaa tcatttgctc acgttcacat ggatctgcga ttacaaactg cttacgaagg
    23461 tcttcttcat agctcaatgc tgactgaatt ttggtttctt tccccaagaa acgatggcca
    23521 cggctcacac gacttccctt gatatcaagg aaatccaagt caaactcttg ctcatccaag
    23581 agaacagtta aagtgtgaac agggcggatg tattcaaagc tatttccagc ccagtgcatg
    23641 ctgacaggga aagtcaatga cttcaagaca tctacaacac caggaacaat ggcttcaact
    23701 gcttgaccaa cttcttcctt agtgacatag acatattctt cacccttgat ttcacggaat
    23761 tcgatatctt caacagtcaa acctttccca cggacaaatc cttgagctgc tttggtgaag
    23821 tttccatcac tatccaaggc aattttcttt gctggtccct tgaaatcttc tgtcaaatca
    23881 gactgtttgt ctgataaacc agtcacacga acagccaaac gacgtggtgt tgagaaggtt
    23941 tgaatggctt caaaagacag gcggttttcc ttgaggaagg ctgccatttt ttcgcctagt
    24001 tgtttttcac ttggtgtgac aacataggct ggtaattctt caagaccgag ttctactaat
    24061 aagttttttg tcatgttttt tctccgaaaa gtatcttttt attttccagt ttgccttatg
    24121 tgattggcac gcaagcaacc aacttcgttg tttcattgct aaaattggag ccaaaagtct
    24181 ccaattttgc ctagtatcct tagtttcact aaggatacct tcatcactgc ggcaactgac
    24241 tcagggctca ctctgttcgc ccattttcgt aatttgtcac tctctttatt ctgcgtcttc
    24301 tgctaggagt ttagctcttg tttcttcatc caaaagtggg taacctaggc gtttgcgttc
    24361 tgcaacaaag gttttggcta caacacgggc caagttacgg atacgagcga tatagcctgc
    24421 acgctctgtt acagatacgg caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt
    24481 gagaacatag tcataggcag ggtgtaccaa gccttcttct aatgcacgac cagcttcttt
    24541 ttcaaactta tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa atgaatattt
    24601 tgagtgctca tactcaggct ggataaagat ttctccgtat tttacaccat cagcccactc
    24661 gatatcatag atagagtcta cttcttgaat gtaagaagcc aagcgctcca aaccataggt
    24721 aacttccgca gtcacagggc cagttgccaa tccaccgact tgttggaaat aagtgaactg
    24781 agtgatttcc attccgtcaa gccaaacttc ccaaccaaga ccagctgaac cagttgatgg
    24841 gttttcccag ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga ttcccaattt
    24901 ttccaaagac tcaaggtaaa gttcttggat atttgatgga gaaggcttca tgaccacctg
    24961 gaattggtgg tgttggtaga gacggttagg gttttcccca taacgaccgt cagcaggacg
    25021 acgtgatggc tctacataag ctgcattcca tggctcaggt ccgatagcac gaaggaaagt
    25081 gtaaggactc attgttcccg cacctttttc attatcataa gcctgcataa gcatacaacc
    25141 ttggtcattc caaaattgtt gcaaagtaag gatgatttcc tggaaagtca atttcttaga
    25201 cattgtttac tccttaataa aaaatgattt cttgcgacac gagtctgcct cgtcgattat
    25261 acgaggcaag acgagttagt actgcgtcta gctcagcacc ctagtgctga gcgtggggca
    25321 gtccgactgt aaggaggttt ctgccactga cgcagtgaaa tgtcaatttc ttagacattg
    25381 tttactcctt tttatataaa ttacttgcga catgagtctg cctcgtcgat catacgaggc
    25441 aagacgagtt agtactgcgt ctagctcagg cactctagtg ctgagcgtgg ggcagtccga
    25501 ctgtaaggag gtttctgcca ctgatgcagt gaaatgttaa tttcttagac attgtttact
    25561 cctttagttt ttatatttct ttttcaatta atttctgctg aacaaaccaa tctaacaagg
    25621 gaaaagtttt ttctgtattt gcccataaat gataagaatc aaagaccgct tgtacactac
    25681 ctaaattttc taccaactta tctactgtca aaaagctgcg ccagtattca taaaaaatac
    25741 ttgcatagtt cttatcataa gtcgaagaac caaaatcatt caatgaatgc caaccatact
    25801 tcttctgaaa aagttctacg agagattgat tacaaatttt ttccgcttga aattcctctt
    25861 ctgtcaagaa atacttgcga ctaatatatt caaccatccc ctcttcgaac cagatataag
    25921 agtcataacc atcaaaatca tctaaaaaat gctccgacca atgagctaac tcatgtccta
    25981 caatctgtaa gaggaaattt tcagataaag aatgatagtg actttctatt gcttgagttt
    26041 gctgagaaca ctcgtagtcc atcaattgat acaaatacaa ctctttccaa acagctaaat
    26101 caggcgccat aaccattcgt ctattatttg tataggctgg aacagcgctt tccctaataa
    26161 tctgtgtagc agcattaaaa tctgaccaaa taacggcttg cggtaaatca cagactgcaa
    26221 gttcattctt taaaaaagat tgataatctt tcaacttttc agtatttctt actacaaaat
    26281 cacgaaatgt agctagttga ctatcgtctt ttacaagata aaggttgtcc acggctctcc
    26341 tttctttcac acaatagact gaataagcaa aagaaccgcg tccgacaatc ctaggttgat
    26401 ttaacctagg ggcgtcagaa acgcggttcc accctaattt attacttcat tgtttttgaa
    26461 aatactacag aaagcgccag ctcattattt tgccctactt ggctcccact atcccaagct
    26521 cgcttgaaga acgccataag actttctttc ctgctgacta ttatatcaaa aattagaata
    26581 atgtacaatt ctttttatga ttttctagaa atccacatca tcaactcgtg gagcacccga
    26641 ttgaacagca atcgttttta aggtttctct ctcctcatga ctcagttcaa ttccaaagca
    26701 atcaagatta gcttgaatac gagaggttgt gacagatttt ggaagtggta aaaatccttc
    26761 tgccaagctc caggccaagg ctatctgagc aatcgatttt ccgtgatttg ctgcgatttc
    26821 ttgcacttgc ttgctatcaa atagttctcc ctgaccaaaa ggcccccaag cttccaataa
    26881 aattcccttt tcacgacagt aagctacgac ttgatcttga tacacacctg gcgccaagcg
    26941 aacttgattg accgcaggaa cgatagttgc agtttcaagc aaggcatcca aatgatgggg
    27001 aagaaaattg ctaacgccga tagcacggat tttcccttct tgatagaggt cttccatcgc
    27061 tctccaaact tccgcattgc gagttttcca tgcgtcattt tctctgagcg gttttgggtt
    27121 cggccaatga atcaaataca aatccaaata atccaagccc agtttttcta tagatttttc
    27181 caaagcttga cgagtttgct cataggtttg ctgactattc caaagcttgg tagttacgaa
    27241 catttcttca cgtggaactc cgctatcttt gattgcttga ccaacacttt cttcattctg
    27301 ataaatcgcc gccgtatcaa tatgacgata accagccttc aaggcttcta acactgcatg
    27361 ataggcttct tctccatcct tagccttaaa agttccaaat cccaatactg gaatttctac
    27421 tccattattt aattgatagg tattcatatg cttctccttc tattttattc atcaattata
    27481 gcataagaca ttttttaagt agtactatct tctgtaaaaa ataagtctgt caaatatcta
    27541 acagacctat tttttcttgc ttattcatca cgactatagt acagctcatg agcttttagg
    27601 cgagtattga gcatctctgg tatggtcaca aaggtataac cttgattttt caaatactca
    27661 atgacccttg gcaaggcatt gactgtcgga ctgtgaatat catgcatcaa aacgatagag
    27721 ccgttagcta cttgatgctg aatttctgtc aaaatagatg cttcattttt actcttccag
    27781 tccagactat ccacatccca catgataaag ctcaaatcca agctattgcg aatatcatct
    27841 gtaatagcac cataaggtgg acgcatgagt ttagaactag aacccagcac tttagttagc
    27901 acatcctcag tatcagtaat ctgcttttta gcttcatcaa gagagagttg cgagagaatc
    27961 ggatggctcc aactatggtt tccaacaaca tgaccttcag attttatcct cttcaccaag
    28021 tcctcattcc cagaaacatt tttcccaagc acaaagaaag tcgctttaat atcatattta
    28081 gctagggtct ctaatacctg cggggtcgtt gctggatttg gaccatcatc aaaggttaga
    28141 gcgacaactt tttgatattt cttatcaaag taagattgat acaaggccgc atctttttcg
    28201 agtaagtacg aagattggat aacatcaaag aaagcagata ctggcaaggc tatctcttct
    28261 aaattttcaa gcacaggact tgaataaagg ataatctgac tatccttgta atcaaaattc
    28321 catgcagaca agtcttggtc agagaagctt tttacaatct gctcactctg gtcttgctct
    28381 atttttttat cctctatgaa ggaggtcaac tcttttatca gctgttcctt agccttacta
    28441 gcatctgaaa acagttggtc aagtgtaaaa ggttgcccat cttctgttaa gtgaaccttt
    28501 cctagactgg tcttttcagt ctcttcaacc ttaaaagcaa ctaaatcata gatttgtttg
    28561 gtcacattac gattaacgac tccctttaaa cctgactctt cttgctctgt atagtagaaa
    28621 acaagattgt ccttactttc tagcttgtcc ttaacatctt gatttatcaa ctccctaaca
    28681 gaggaaatca ctttctcccc ttggagagga taataggcaa tcacttcggc ttgcccctga
    28741 cgaaaatgct ccttctgatt tccctcactc aattgatcat ctttctcttt tttgagcgat
    28801 tcaatctttt gctcaaaact tttttgctgg tagatcttaa aagcaataaa tccgcctaat
    28861 aggcaaatag aaattgctaa gataccaatc aaagccaata atacatgtct cgttttcccg
    28921 tgtctgccac gtcctagtct acttttattc atatttatat cataacaaat ttgagcaata
    28981 atttcaatga tacaatttgg caatccacaa ttcctctagc aagccaaagc atcttacatc
    29041 aactccaaca tccgtatctg tagagattat gtagttttct atctatggaa tttctagtaa
    29101 attgaagcta gaatagtaga cctatacttc taaaacattg ttagaaatcg atttgattgt
    29161 cctgatcgat ttgtcctgtt cttatttcat tttactataa aactatgaaa agctaggtca
    29221 tctgagaagt aattactatc gacaattgcg atagcgtata ctggtgcgat ttgtttgtag
    29281 ctctggtggg tatcaccttc acgttggcga attttttcta ggttttgatt aacctgactg
    29341 cacagataag cccataggcg attgatgaaa aaattctgat gatgaacctg aatttcgatg
    29401 ataacttgga ttccattatc taactccgcc aagacgtcta tactagtata gaaatcctgc
    29461 gctgagtagg acatggaagg caagacgtga atgttacttc cctccaaaat cgtcacattt
    29521 tttgctggca agtccagcat atcgcggata aattgacaaa tcattagttg ggctgatacc
    29581 cggatgtctg agaatcctcc ttttcctcct tctattatag cacattttta aatctaggtt
    29641 tgctagattc tatgcttcta tctatttatt cggaaaagaa agcttttctt cagaagaaag
    29701 ctaatagctt atttgaaatt ttctcctctc tgttggatag tcccgtctat ctatgttata
    29761 atgaaagaag gatttaaaat cggaaaagga gtatttatgc taaaattagg tgtcatcgga
    29821 actggcgcta tcagccatca tttcatagaa gtagcccata ccagtggaga ataccaactg
    29881 gtcgcaatct attctagaaa actagaaact gcagcaacct ttgcttctcg ctatcagaat
    29941 atccaactat tcgatcaatt agaagtcttc ttcaaatctt cttttgattt agtctatatc
    30001 gccagtccaa actccttaca ttttgctcag gcaaaagctg ccttgtctgc tggtaaacat
    30061 gttattctcg aaaagccagc tgtcactcaa ccacaagaat ggtttgattt gattcaaaca
    30121 gctgaaaaaa ataactgttt tatctttgaa gcagctcgta attaccacga gaaagccttt
    30181 actactatca aaaacttttt agcagataag caagtgttag gagcagattt caactattcc
    30241 aaatactctt ccaagatgcc tgacttgttg gctggacaaa caccaaatgt cttttcagac
    30301 cgttttgctg gtggagccct catggatttg gggatttatc ccctctatgc tgctgttcgt
    30361 ctttttggaa aagccaatga cgcgacttac catgctcaac agcttgacaa tagcattgac
    30421 ctaaatggag atggtaccct cttctaccca gactatcaag ttcacatcaa ggctggaaaa
    30481 aacatcactt ccaatcttcc ttgcgagatt tatacaacag atggaacctt gactctcaac
    30541 acgattgagc atattcgctc agctattttt accgaccacc aaggaaatca agtccagctt
    30601 cctatccaac aggatcctca tacgatgact gaggaagtcg ctgcatttgc acacatgatc
    30661 cagcaaccag atctgaatct ctaccaaact tggctgtatg atgcaggctc tgttcatgag
    30721 ctactatata ccatgcgcca gactgctggc attagatttg aggcagaaaa atgaaaacca
    30781 aactaccgac tgaatggcaa gaactgagtg accaactcgg tttccaagaa ttcaccctca
    30841 ttcaaactca actatttgag ccccttcttg ctggagaaaa cctcctagga gtgagccaaa
    30901 ccggaactgg taagacccta gcgtatctcc taccaagtct tctcagacta caaaagaaaa
    30961 aagcccaaca actcttgatt ctagcaccaa atacggaact tgctggacag atttttgatg
    31021 tatgtaaaac gtgggcagaa gctatcggat tgactgctca actcttcctt tcaggttcaa
    31081 gtcagaaacg acaaattgaa cgccttaaaa aggggtcaga aattctgatt ggaactcctg
    31141 gccgtatctt tgaactcatt aaacttaaaa aaatcaagat gatgaatgtg gaaaccatca
    31201 tcctggatga atttgaccaa ttactagatg attctcagat tcactttgta gaaaaaatca
    31261 ctcactacgc acctcgtgac caccaactgg tctacatgag tgccacgacc aagtttgacc
    31321 aagaaaagat tgtgccgaat acacgcacta ttgatctctc tgatcaaaaa ttggacaata
    31381 tccagcattt ctacatgcag gtagaccaac gtcatcgagt ggatatgcta cgaaaactgg
    31441 ctcatgtaga ggatttccgt ggtctagtct tcttcaatag cctgtcagac cttgggaatg
    31501 ccgaggaaaa actacagtat cgtgatatat tggctgtttc cctcgctagt gatgttaatg
    31561 ttaaatttag aaaaatcatc ttagaaaagt ttaaagataa ccaactaacc ctgcttcttg
    31621 caactgacct tttggctcgt ggaattgata tcgatagcct agaatgtgtc gtaaactttg
    31681 atattcctag agatagcgaa acctacacac accgtgctgg ccgtactggt cgtatgggca
    31741 aggaaggtta tgttatcact ctcgtcactc atccagaaga aatcaaaaaa cttaaaaagt
    31801 ttgcaagtat ccgagaaatt gtcctaaaaa accaagaact ctatatcaaa taaagcccta
    31861 ttgctctata cacatccagt gtatggagct tttttataag caattcaatt ttatactcat
    31921 ctgctttcaa aaagcattct agtccatctc cgattaacga tggactttat cacctccttc
    31981 tccagtcctt gtatgacatc ttccaaagtt cgaaaggctt tattcttaaa tccacgttta
    32041 cgaatctctt tccacacttg ttcaatgggg ttcatctctg gtgtgtatgg aggaataaat
    32101 gcaaagccaa tattagtcgg aatctttaag gtacttgatt tatgccatat agcattgtcc
    32161 ataacgagta aaaaataatc atatggataa gcttgtgaaa gctcttctaa aaagacgttc
    32221 atccactcag tattacatcc accagctatt aagaaaaatg attcgcttgt ataggcatca
    32281 acagctccat aacaattgtg gacctactcc tattggagac caacaagatc ccagtttact
    32341 gattctaccg aaaccagcct catcttggta catcaagcga accttatgaa aacatctact
    32401 agttttaaag cgctttcttg tcttcttgaa ttgagatttt atttttagac gcgacaatgg
    32461 tttgagcgtc tgctttccta ggatgttctg gacgtggcgt aatatttcgc caaccatggc
    32521 gcttcaacag ttgatagaag gcatcacgtg tgtaggaacg acctaactcc tttttataag
    32581 cctgaaataa ggcatcaatt gtaacaaatt ctcctgcctc tgcagccttc aaatggcggg
    32641 caagaaagac tttcttttcc tcaactgtca tatatgcatg gttacgacca ccacgtgttt
    32701 cttgaaggag agagtcgagt ccgaactcct catatttttt tacattttgc taaatcgttg
    32761 tttgattaca gtctaaaagc tctataatct ctttataaga tttgcccatc agacgaaata
    32821 gaacgatttg aaggcgttta tgatatttag ctgtacgaga gtcttttaaa agtgttttga
    32881 tggtttggat ttcttcttta gttgatttca tattactatt gtataatgct ttttgatttg
    32941 agtctagtat tatactcttc gaaaatctct tcaaaccgcg tcagcttcgc cttgtcgtat
    33001 agatgttact gacttcgtca gttctatcta caacctaaaa acagtgtttt gagcagtctg
    33061 cagctaactt tctataacaa aatgaaataa gaacaagaca aatcaatcaa gacaatcaaa
    33121 tcgatttcta acaatgtttt agaagtagat gtgtactatt ctagtttcaa tatattatag
    33181 tttgctcttt gattttcatt gagtattagt tcaatatcta aaaaaagaac cttgccaagc
    33241 aagattcttt tagtgtctga cttaaataat tgttcttctg ggaactaaat cgaattaagc
    33301 ttcgatttct gtaaccatac ctgaaccaac agtacgtcca ccctcacgga tagagaatgt
    33361 agtaccttgt tctacggcga ttgggtgaat caactcaacg tcgattgtca cgttatcacc
    33421 aggcattacc atttcagtac ctgctggaag ttcgattgaa cctgtaacgt cagtagtacg
    33481 gaagtagaat tgtggacggt agttgttgaa gaatggagtg tgacgtccac cttcttcttt
    33541 agtaaggatg tagacttcac ctttgaattt agtgtgtggg ttgattgaac ctggtttagc
    33601 gataacttgt ccacgttcga tttcatcacg ttgaacacca cgaagaagga cacctacgtt
    33661 atctccagca agaccttcgt caagttgttt acggaacatt tcaacaccag taacaactgc
    33721 tttttgagtt tcttctttga taccaacgat ttcgatttcg tcgttgactt taacgatacc
    33781 acggtcgata cgtcctgaag caactgtacc acgtccagtg attgagaata cgtcctcgac
    33841 tggaagaagc aatggtttgt cagtgtcacg ttctggttct gggatatact catcaactgt
    33901 gttcatcaat tccataacga tgtcttcgta tttagagtca ccttcaagag ctttaagtgc
    33961 tgaaccttgg ataactggaa gatcgtcacc tgggaagtcg tattctgaca ataggtcacg
    34021 gatttccatt tcaaccaatt caagcaattc ttcgtcgtca accaagtcaa ctttgttcat
    34081 gaagacgata aggtgtttaa caccaacctg acgtgaaaga aggatgtgct cacgagtttg
    34141 tggcattggt ccgtcagttg aagctactac aaggatagct ccgtccattt gagcagcacc
    34201 agtgatcatg tttttaacgt agtccgcgtg tcctggagcg tcgatgtgag cgtagtgacg
    34261 tttttcagtt tcgtactcaa cgtgcgcagt gttgatagtg ataccgcgtt cgcgttcttc
    34321 tggagcagca tcgatagacg catagtcttt aggttggtta actgatgaag gcaagcgacg
    34381 tgccaaaaca gttgtgatag ctgcagttag ggtagtttta ccgtggtcaa cgtgtccgat
    34441 agtaccaatg ttaacgtgtg gtttactacg atcgtatttt tcttttgcca tttgagtaaa
    34501 agcctccaat aaaatatatt ttatagatag acagtaggca atacagtcta actttcctta
    34561 ctattttatc aaatttcagc taaattgcaa gtgttttaca acgtttttgt gaattattct
    34621 tggtaaaaat ttaactaatt aatagaaagt actaaataga cttatccttc aaggagtttt
    34681 cagcattatc catccactca cttgctgaaa atgtaaacag tctctggttt atatagtaaa
    34741 atgaaacaag aatagggcaa atcgatcagg acagtcaaat cgatttctaa caatgtttta
    34801 gaagtagatg tgtactattc tagtttcaat ctactataca acctcaaaac tgtgttttga
    34861 acaacctgcg gctagcttct tagtttgccc tttgattttc attgagtata aaattgcaga
    34921 taaaacgata acaatcatag gactcagcag agtcaatgta ggtcgaagaa tagcccccct
    34981 aatcgcttat tttatagcaa aatgaaatag aaactgaaca ttttgttgag gaaggtaaat
    35041 caattcctca caatgtttga gaaagaaggg gggactattc caacttcaac ccactataaa
    35101 gcagacttac aagataggga acccatctcc gataatattc cgataatata tagtagaatg
    35161 aaaactgcac aatttgattg gagaattcaa accaatttct aacaatgttt tagaaacaag
    35221 ggtctattat ttcaactgca acctactata gcccattgaa gctggaatag cccactgaga
    35281 cttctaaacc actgctggag aggagtttgt ttagagtttc tttcatttcc ctaaaaaatc
    35341 aaaaagagag aaccagtctg atcctctctt acttatagat ggtgctcact caaatgttaa
    35401 aggagctatt tcttagagat aaaggaattt gaatacagct actgccgcaa ttgctgctgc
    35461 gataggtgct actactggta cccaagaata ccaccatttt gaatcgcctt tatgctcacc
    35521 aagaactgat tttggaagga aagcatgaag gagacgtggt cccaagtcac gggctgggtt
    35581 caaggcaggt cctgtaggtc ctccaagtga tgttaccaaa gccataacga ggaatccaag
    35641 tgccaagtga gccacagaaa gtcctgaagc agtgtgtgga gccacctgtg ctttaatagc
    35701 caagtcagaa aaatcaactg tttgtcctgc ttctattgcc ttttgtttca tgaattgaag
    35761 cacttcagca ccaaagaagt ttttagtcaa accaagagct gcaaagaaca aaacaaatga
    35821 accaacaaac tcattaatca aaccattgac agttgctgca tagcgacttt cttttgtacc
    35881 atggtcaata cttgaaatag ttgagaaagt tcccaagatg ttatttgggt tttcagtttt
    35941 caagtagaat ggacggtatg ttgccacaac taaggcttgg ccaaagatag cccccaagac
    36001 ttgcgcgata atgtaaggta ccacttgtgc ccaagggaaa agaccgctaa ctgcaagccc
    36061 tagagtgaaa gcagggttga tgtgattccc agatacgtta ccaaacatca aggctgggat
    36121 cataaccccc ataccataac caacagcgat gacgatccag ccactttggt gacctttcgt
    36181 acctttaagt tcaacgttgg caactgcacc attcccaaga atgatcaaaa tggcagttcc
    36241 caaaaattca gtggcatact tcagtgccca tgtgaaatcc atttgctaga ttctccttaa
    36301 aattttttag atattcctta ttttatcaat ttttacccta tttagcaact gatttctgga
    36361 aatattttta aaaaaatagg attcatagtt tcccagttca acggacttgt gagcgttttt
    36421 attcttcacg aaagacctcg ttctcaaaac caggaaaaaa gcccacatct gtgagctttt
    36481 cctggttcct agattctaaa atctattatt tttcatcttc ttttttcttg aagaatccta
    36541 atactagact agctaacaag gtcatgatac cagcactagc caaggtagca ttagcttctg
    36601 tacctgtgtt tgacaatccc tctctcttac ctgtctcata agtcggaact tctgggtcta
    36661 gattcactgg agtttcagtt tttggagtac ctggttctgg agttggctct ggtattggag
    36721 tctcttgatt tggtagttct gggtttggtg tcggaacact tggatgctct ggatttggtt
    36781 gtgggattgg tttttctgga gttggtttat ctggtgttga taaacggtca taccttaccg
    36841 ttatttcttt atcactagag tctgacgtaa cttcttgtga ttcaactgtt ggaatatctg
    36901 gagctttgta cttgtcaatc ttaccagata taacctcgtc ccagtttcct gttgtccatt
    36961 caccgtaggt tacaactccc gtgaccttgt tctcagtttt tgtacggctt aaggttacag
    37021 gttgaacaac atcttttttt acattttggt tcgtaacttt atcaacgtaa tgaatgatac
    37081 gcgttatagt cttcgtctca gtagaggttg ctgttttggg aaccactgtt tcctcaacat
    37141 tctcacggta gtaatagtca actgttgcac cgtcttctgg tacgcatttg caggagttgc
    37201 taccaaggtg tatgttgttt tccttgtgat aactcggtct tctttgtcct cagttgttgt
    37261 tttcccttca atagtttttg attctgtggt atactcagaa cctatcgcta aatcagcttt
    37321 tataacagac tctgccaact tctcttggct accttcttta tagtaattgg ctgttactgt
    37381 agcagtggtt ggcgcttcgc tttctctata aactaaggtc actgttctac cttcgcttac
    37441 aatattccca gttaaacttg cagaatttgt atctgcttct ttaaaagtat aatattttcc
    37501 gtcagtagta gtcatgctac tgagtttttt atctgtgaca ttgtagctgg taccaatcag
    37561 ttgtttttta ttggtaatgt aggttccgtc actttctttt tctccaattc cagtatcatt
    37621 tttcaatgat agcaaacgcc cttgttcatc aacatagcga actttcacat tttctgagat
    37681 tagttctgcc aattctgagg tttttttctt tttcttgatt tcttcggtta ttttcccttt
    37741 ctcttcttcg ggaatattta gttttggaat gattttttca ataacggttc gtgatggttc
    37801 cacagtatct tggatgactg aaaagtcagc tagaattggg agattatact gaacacggtg
    37861 actttgagtg tttactccta ctctttcatt attctctgaa aatacttgta cggtataaga
    37921 aacaacatct tttcctaata gaacatcccc agtagagaaa tagccgcctt ttcctagttt
    37981 gctatctcca gagcccactt ctttcctaat cttatcagat agttttttac cagtcagtac
    38041 attcgttcgc acaatccctt tgtctacccc tacaaaatgg gagaactttt tgaactcttc
    38101 agaaccagat ctagcccaac cattattaag ggcatttgct tttgtatttg tattctctct
    38161 caaaggtttg gcgattagaa ttatattcat cggcacttag agttgctgct atatctgact
    38221 cttgaatacc aacttcctta ctaccatttc tagcggcagt atatgtgtat ttatctgttt
    38281 ctacttctag ctgattatcc ttaacatgat aggtaatttt ctcctggtat aagagtcttc
    38341 agtagtttat atgctctgtc atcatcttta gctttagaag cagctgagtt tagccaagtt
    38401 ggggtattag gagtatcttt atcagcctgt ttcaagacat tattcaagtc atttgctagc
    38461 ctattggtta tcctcactgt gaaagttttc ttccagtctg aaccactttt attagactta
    38521 gatatgctca tgaaatcctg gtcaacattc aacacaatat cgccttgttt tttagcatct
    38581 tccgttgctt ctgcttcaaa ttgatcatgg agtttttgtt gctctggtgt atagtttttt
    38641 tatttacacg atttaatgta ctatttttta ttttagtatt aagaactcca ttaatacgat
    38701 ttttaaattc tgcttcaaaa aatcccttaa tttgttggtg cgcaagttca acattcaaac
    38761 ctttcttata caagtcttca ttctgataca agtcttcatt ctgaacgatt tcggctacac
    38821 ggtgaatggt ttgattgtat accgtcttct cttaaccagc agtatcctta gttttaatga
    38881 ctactcgata ggttccttta gaaccgttat agtcatctag ttttaaaaaa gcattccaaa
    38941 cattcaaaaa tagttggaac ttcccttaat aaatccgtca ctttcttttt caagatagcc
    39001 caagcttgct caataggatt caaatccggt gaataaggtg gtagaggtaa gaaaaagtgt
    39061 ttatcttgga tgcagagttc atccaaaatg ttcttgggat gaaaactagc attgcccatg
    39121 acaataacat gaggtgtctt caaagcaggt aggagttgcg ttttgaacca ctccacaaag
    39181 aaatcgcttg tcatgctttt cttgtaaatc atgggagcta taaactctcc gtctacttgt
    39241 cctgcaacaa ttgaagttcg ctcaaaacga cgtccgctaa tcttttcata gactttctcc
    39301 cctctaggag cccgtgcata aggacgatag aggtagcggt cgattcctgt ttcatcaata
    39361 tatacgattg gtaaatcttt taggttatcc aaaatatcaa gaaactcagc gactttctct
    39421 ggctcttgtt ccttaaagct cgtcatcttt ttttaaagtg accttaatat actttaaagc
    39481 tgcccatacg gagggcagag cacaatcaaa atgtgccgca atttcccgta aaaaagcatc
    39541 tggatgagcc tctacaaagg ctttcaattc ctctaaagga atctttcggt ttttgacgac
    39601 tcgctttttc ctctctatgt gtccttgttc acatctttct tttcccacgt gaagagagtt
    39661 ctgacgccaa caccaaaaaa cttggctgcc tcgacatggc tgtgcccctc tttgatggaa
    39721 tctaatgctc gctgtttaaa atcagtacta tatgccatag ctttattata atatgcttgt
    39781 ttaaaactaa gcaactatat caatgttttg ttattagaag acttactttt tttactgaaa
    39841 tcgaattttt cctaacctca acatcatata accccaaaat tcaatctgac ctggtttgaa
    39901 gagatgttcg aagcagataa ggttttacta aaactaaaaa aaatgatata atcaaactga
    39961 ttaaatgttt taaaaataaa ggagaatttg aatgtcttac caagaaaatt accagaaatg
    40021 ggttgatttt gtggagcttc ctgactacct tcgtcaagat ttggaaaata tggacgaaaa
    40081 aactaaggaa gatgccttct atacaaatct tgaatttggt actgcaggta tgcgtggctt
    40141 ggttggtgct ggtacaaacc gtatcaacat ctacgttgtt cgccaagcta ctgaaggatt
    40201 ggctcgtttg attgagtcaa aaggtggaaa cgagaaagaa cgcggtgtag caattgccta
    40261 cgatagccgt cacttctcac ctgagtttgc ctttgaatct gcggcagttc ttgctaaaca
    40321 cggcatcaaa tcttacgtat ttgaaagcct tcgtccaact ccagaactat catttgcagt
    40381 tcgtcatctc aactgtttcg caggtatcat ggtcacagcc agccacaacc ctgctccatt
    40441 taacggttac aaggtttacg gtgaagacgg tggacaaatg cctccacacg atgcggacgc
    40501 tttgactact tatatccgtg caatcgaaaa cccatttgca gttgaagttg ctgatgtgga
    40561 aactgaaaaa gcttctggct tgattgaagt tatcggcgaa gctgttgacg tagaatacct
    40621 taaagaggtt aaggacgtaa acatcaaccc agccttgatt gaagaatttg gtaaagacat
    40681 gaagattgtc tacacaccac ttcatggtac tggtgaaatg ttggctcgtc gtgctcttgc
    40741 ccaagcagga tttgactctg ttcaagttgt tgaagcgcaa gcaactgctg acccagactt
    40801 ctcaactgta acatctccaa acccagaaag ccaagcagcc tttgcccttg ctgaagaact
    40861 tggtcgtcaa gttggtgcag atgttcttgt cgcaactgac ccagacgctg accgtgttgg
    40921 tgttgaagtt cttcaaaaag atggtagcta cctcaacctt tcaggtaacc aaatcggtgc
    40981 tatcatggct aaatacatct tggaagctca caaaaacgct ggaactcttc ctgaaaatac
    41041 cgctctctgc aaatctatcg tttcaactga cttggtaacg aagattgctg aaagctacgg
    41101 cgcaactatg ttcaacgtct tgacaggttt caaatttatc gctgaaaaaa tccaagaatt
    41161 cgaagaaaaa cacaaccaca cttacatgat gggatttgaa gaaagcttcg gttacttgat
    41221 taaaccattt gtacgtgata aagatgccat ccaagccgtt cttgtcgttg ctgaacttgc
    41281 tgcctactac cgttctcgtg gtttgacact tgctgacggt atcgaagaaa tctataaaga
    41341 atatggctac tacgcagaaa aaacaatctc tgttactctt tcaggtgtcg atggtgctga
    41401 acaaatcaaa gcgattatgg ctaaattccg caacaatgct ccaacagaat ggaacgcaac
    41461 agctatcact gtcgtagaag acttcaaggc acaaactgct actgttgctg acggtactgt
    41521 tacaaacttg acaactcctc caagtgatgt gttgaaatac acacttgctg acggttcatg
    41581 gattgccgtt cgcccttcag gtacagaacc aaaaatcaag ttctacattg cagttgtagg
    41641 ggaaaccaac gaagaatcac aagctaagat tgctaacatc gaagcagaaa tcaatgcatt
    41701 tgtaaaataa agacctataa aagatgagac caaccaacct catctttttt cgtatcaaat
    41761 ctaagcaatt ttagaaactt tatggcatac tagacttatc aatgaaagcg aggtaatctc
    41821 atggaacaat ttttagataa catcaaagac cttgaagtca ctacagttgt gcgtgcgcaa
    41881 gaagctcttg ataaaaaaga aactgcaacc ttctttatcg gtcgcaaaac ttgcccttac
    41941 tgccgtaaat tttcaggtac attgtcaggt gtcgtagctg aaaccaaagc tcacatttac
    42001 ttcatcaata gtgaagaacc aagccaactc aatgatttgc aagcattccg ctcacgctat
    42061 ggaatcccaa ctgtaccagg ttttgttcat attacagatg gacaaatcaa cgtccgttgc
    42121 gactcttcaa tgtcagcaca agaaatcaaa gatttcgcag gattgtaaaa aatcagctaa
    42181 caagtcttaa aatcacaaaa acgcataatg taaggtgcaa aaaccttgat attatgcgtt
    42241 ttattgttcc taaaacagtt acatcagcat ttcattacag aacattttaa ttttggaagt
    42301 caaatcaatt tttaacaatg ttttagaaat cacagtgggc tattccatat tcaattatcc
    42361 agattcaatc gctattatcg ttttcctaga ttacaaaaaa aactacgttt tgctaatcaa
    42421 aacgtagtct cagccactat tttatcttac tgtccttctt ttacttcttt ggttgctaca
    42481 tcttctccaa accatttttg gctgatttct tggaacttgc cgtccttgta aagactagaa
    42541 aaagcttcat ttatcttctt aaccaaggtt gtatcttcct tacgggctcc aaccgcaaaa
    42601 gcttctgttt ctagtccaac tgtaaagaca ttataatcgt ttaaaacacc ttctgcttct
    42661 aaataatagt ttgcatagac acggtcaatc aatagaccat caattcgatc gtttttcaaa
    42721 tcaatcaagg cttcattaaa ggtttggtat tgattcgctt ccttattagc gacaatattc
    42781 ttcaaaagtt ctggatttgc ttcaaagtcc gcataaccag atgaaccagc ttgagctcct
    42841 aatgtctttc cagtcatatc ctttgcagtc gtgataccag atgatttctt cgtaaccaat
    42901 acctgctcat tcttcatata tgagttactg aaagccacct tttcacggcg ttcgtctgta
    42961 gcggaatagc cattccaaat cagatcaatc gttccttttg tcaattcagc ttctttcaaa
    43021 tcccaatcaa tcggttgcca atttaccgtg attccgtatt tttcaaaaac agctgtagct
    43081 aaatcaatat caaatcctgc ataagaacca tctttctgag caaatcccat tggaacaaaa
    43141 gtactatcaa atccaatagt aatagacttg ttagactggt actttgacca attatctcca
    43201 ctagtttcgc tagaattttt cccacaagct actaagaaca aagcagtcat cagactgact
    43261 aatacaagca tccatttttt catccatttt tcctcctatt taggttctac tttcaataat
    43321 acatctgcga tattttcagc aaactgcaaa tcatgggtaa ccacaatctg ggtcatccca
    43381 agttccctat tttgcaagat tagcttctcc acttccaaac gtaattctgg atccagggca
    43441 gaagttggtt catcgtagcc aatgatttct gggtcaatca tcatagcacg cgccaaagcc
    43501 actcgctgct tttgcccacc agatagtgag aaaggatagg cctctgcgtg tcctcctagt
    43561 cctaactgtt ccaagagtcc actcgccttc ttctcagcct cttcctgctt cattcccatg
    43621 gtcttcacag gcgataaagt caaattttcc agaactgata gatgaggaaa tagttgaaaa
    43681 tcttggaaga caaatcccag taggttgcgc ttctgcaatt catccagctc taaaggttgt
    43741 ccattataaa agatttgccc tgaatcaatg gtttcaagac ctgcaagcat acgtaaaaga
    43801 gttgtcttac ctccaccaga aggtccaacg atagccagga tttgcttttc aggaatactt
    43861 agactgaaat tagacaggat ttgtttgtct ccaaagactt tattgatatt tcgtaattct
    43921 aacatggcag cctcctatct ataataacta tacttcttct caactttttt ggaaataatt
    43981 gtcacaatcc caatcaaaat caaataaatg gctcctgcca agaacatagg aactagacta
    44041 gcatcgcggt tagcagctgt tcgactagcc aagataaggt ctgaaattcc gagagcatag
    44101 accaaagaag tatccttgac caaactcata acttcattaa agacactagg aagaacaatc
    44161 ttggtcactt ggggcaagat aatatagcgc actctgtcaa aagggctaaa ctttaagacc
    44221 ttggcagcct catactgtcc tcttggaata gtgtcaattc ccccacggaa aatttctgca
    44281 aagtaagctg catagttgag aacaaaggca ataatagctg caggaaggcg gtctaaacga
    44341 atcccaatac ttgggagcac ataatagata aaaatcagtt gcaagagtaa gggggttcct
    44401 cgcataaccc agatgtaaat gttaatcaga taatggaggg gcttccaatg gacttgcaag
    44461 gcaaaggcaa tcaaaacgcc caagggaatc gaaaatagca agaccagtgc aaagacctgt
    44521 acagtcgtgc tcgcaccatt cagtaaactc ggtaatatct caaataaata agacatactg
    44581 cacctcctaa aagtaattgt tcctattata gcatgaataa acaagattac caagtatttt
    44641 tgtaaaaaga ttctaccata tacaaataaa aaccgtagct atcaaaagag ataaactaca
    44701 gttctatatg tttaaacatt cttacaatct ctcaaacagt tcttgcattt gcacatcatc
    44761 tggaaccagt tttaagtaag tgtgagcatg gacttttgct tcttcaaaat gtcccaattc
    44821 acgcaagaga tagatatagt gttccagaaa ttctggattg tccttcaaat ctcctgtcaa
    44881 ctcttgataa tgctcataag cagtatccaa atcgtccatt tcttgataag aacgagcaat
    44941 catccacttg gtcaaaagat tttctggctc ctcactctgc aagtctagaa tatcctcata
    45001 acgctcctgc tccagataaa tagtggctaa acgaagcaag atttcttctg tatcctcagc
    45061 gtcttctttt gcagtaagga gataattttc tgcaccacta gcatcatgca attcataaga
    45121 aaattgtgaa gcagctagca agaggcgagt ttcaaaggga tttttctcta atccttgctt
    45181 agcgatacgc agggcttctt gaacttgatg ttccttatgt aaagcctgac tgtacccata
    45241 ctcatagcct tcaaagtcag gagaaatggt atcaagctgc ttaaagtaga gggtggcttt
    45301 ttgatattct tcttgatcaa aataaagact ggccaactca aaagctgtta agtcatcgta
    45361 ttctaactcc agggcttttt ctaaaaactc agtagccgtt tcaaatttcc ctaactgagc
    45421 ataggcaaag ccaattcgtt gataggtgga aatgcccgtt tgctcataaa tcgagcgatt
    45481 atctaactgg gcataggctt gaatagccgc ttggtaattt tccaactcac tatccaactc
    45541 tgccaaaccc aatatcaaga gagaatcctc tgagtaggtc aaggcctcca ataatttctc
    45601 acgtgccaca tctgtcaaac cttccagctg gtaaaggtct gccttcaaaa ccaaagacga
    45661 gacataccag tcactgtcag cttggatttc ctcaagatag gtaaaggctt cttctatttg
    45721 accatcctcg ctagcaattg cagctagatt aagatgaacc tctggaaaat cctctacaat
    45781 tttcaggtaa atttccttgg cctgaggata gaaaccaatc ccttcaagat aagttgccaa
    45841 ctcatacaga agattacttg aatcattttc taaagctttg gcgaaataat gctcagcctt
    45901 agttaaatct tgctcctcca aagcctgtaa catctgttga ctattgttca ctcttgactt
    45961 cctcaccctc taattcatat agaccactga ctaccttata ccattcgaaa atggctgaaa
    46021 tgacaacctt agcagaggca taaaccggaa taccaagtaa aactccccag ataccaaaca
    46081 tagatcctga agttaacaaa acaaagagaa cattaatagg gtggatgttt aattgacttc
    46141 ccaaaatcaa tggagagaca aaacggcctt caatagtttg ttctacgata aagacaatca
    46201 ctactttcaa aagcatgact ggaccagcaa tcaaacccaa tactagagca ggaagcatgg
    46261 ctagaaagct accaagataa gggaccagat ttaaaatacc agcagtaacc cccagcgtaa
    46321 ccgcatagcg tagaccaata atcttgaaga agatgataaa cattactgct acaataatag
    46381 ccactgtcac ttgccctcga acatagttgg acaactgttg attcacatct gatagaactt
    46441 gtccaacagg ttccttcaat tttcttggaa tgaattgggt caaatagtta cgcaagcctt
    46501 tcccatcacg caagagataa aagagcatga aaggaacgat aatcaaggca acaatcactt
    46561 gagaagcccc gctaataaag gcactcaccc agttgactgc ctgagatgaa accttacttg
    46621 ccaaaactgt agcctggcta gaaaaattgg tcaaaacttg ctctaattga ggtctgaaat
    46681 catctggcag atgctgggct accaatccat taacaatcct atctatatct tctaagtaaa
    46741 caggaacgtt tcttgcaaag gtcaaaacct gacgttgcag atttggaatg gcgactgcca
    46801 agccccaaat gataaagaga gcgatgataa caaagacaat agtgatagct ataacacgat
    46861 taaccttatg cttctccatc caatcaacaa taggattcaa caaataatat aacaaaccag
    46921 acaaaatgac tggcaacatc acaactgcta aaaagtctaa aacaggtgaa aatagaaaac
    46981 taatcttact taaaataaaa agattcagtc ccaataataa ggttactaaa aataccgtaa
    47041 ttgccttgtt atctaaaaac cacttgaaaa accaagatag gctaaaatgt ttctcttttt
    47101 gctccataaa tacttccttt ctattgttct ctcattatac catttttgaa acaaaataac
    47161 tcttttttaa agtgcttaca tctaattgga tatctttaat ttacttgttt ttgtgatata
    47221 atagtcttat ctttacacag aggtatagta atgaaagaaa ctgtttattt tggaacttat
    47281 acacgtcgta cttctcaagg gatttacaag gcagactttg atacagaaac tggtcagctt
    47341 tcaaatctag aactttttgc agctgagcca agtccaacct accttgcctt tgaccagcac
    47401 caacatttat acactgttgg tagccaagac gataaggggg gaattgcagc ctatcaaact
    47461 gacgggactg tgttaaatca tgttgttgaa gaaggagctc cccactgtta tgttgctgtc
    47521 gatgaaaagc gtgatttggt ttacgcagct aactatcaca agggacaagt ccttgtttat
    47581 aaacgccagg aagatggtag tcttctactt agtgatatgg atcaacacag tggccaaggt
    47641 ccacatgaaa atcaagcttc cccccatgtt cactatacag atttaacacc tgaccactat
    47701 ctagtgacct gcgacttggg aactgaccaa gtcatcacct atgacctcga tcaagaagga
    47761 aaattatcta agctctatac ctatcacagc aagccaggag caggctcacg ccatatcatt
    47821 ttccataacc actataaaat cgcttatctc atttgtgaac tcaatagtac tatcgaagtt
    47881 ttaatctacg atggcgttgg cgaatttgaa cgtatgcagg tcatttcaac tttaccggaa
    47941 gcttacgaag gctttaatgg aaccgctgct attcatctct ctaaagacgg taaatacctc
    48001 tacgcttcta accgtggcca tgattctatc gcagtatata ccatccttgc ggacggtagc
    48061 ttagagttat tagaaatcgt tccaacgcat ggtcagactc cacgtgattt tgatttgaca
    48121 cccgaccaaa aatttctcat tgttgtccat caagactctg acaatgcaac tgtctttaaa
    48181 cgtaattgtg acaatggtcg tctagcagaa ctctccaacg acttccatgt tcccgaagca
    48241 gtctgcatcc gttttgctcc ttaaaacacc ataaaaaatg aacttggtat tttcaagttc
    48301 attttttctt atagtctatt tccgacatta atacggttaa tagcacgttg caaagcaatc
    48361 ttagcacgac gttcttggtc aatcaaatgt ttgtcttgtg cttcttcaat tgcacgttct
    48421 gcacgaagtt tggcacgttc tgcacgactg atatcgatat cacgagcacg ttctgcagag
    48481 tcagcgacga ttgtgatcat atcattggca atttcaataa cgccaccgtt tactgcaatc
    48541 cagttcacgt gatctttatc atcgatacgt tttaccttta cttcatcaac tgctaaaacc
    48601 gcaatcatat tttcatgtcg tggcaagatc cccatctcac catccagagt tcgaaccgat
    48661 acatagctgg catggtgatc atagacgaga ccatctggtg tcacgatctg gacagttaac
    48721 tgagccatag atcacctctt aaaatcccat tttttcagct tttgcaatca catcttcgat
    48781 agaacctaca ccacggaagg catcttctgg caagtggtcg tatttaccat caaggatttc
    48841 cttaaagcca cgtacagttt cagcaactgg aacataagaa cctggctgac cagtaaattg
    48901 ttccgcaacg ttgaagtttt gtgacaagaa gaactggata cgacgggcgc gagcaaccaa
    48961 ggtcttttct tcatcagaaa gctcatccat accaaggata gcaatgatat cttgcaattc
    49021 atggtaacgt tgaaggacac gttttacttc agcagcaact gcatagtgct cttctccaac
    49081 gatttcaggt gccaaggcac gtgagcttga agcaagtggg tcaacggctg ggtagatacc
    49141 caattgtacc aacttacgtt ccaagtttgt tgttgaatcc aagtgagcga aggctgttgc
    49201 tggcgctggg tcagtatagt catccgctgg cacatagata gcctggatag aggttacaga
    49261 acccttcttg gttgatgtga tacgttcttg caattgaccc atttccgtag caagtgttgg
    49321 ttggtaacca acggctgatg gcatacgacc caaaagggca gatacttctg aaccagcctg
    49381 agtgaaacgg aagatattat cgataaagag aagcacgtct tggccttcca catcacggaa
    49441 gtattcagcg attgtcaaac cagtaagggc aacacgcata cgtgctcctg gtggctcatt
    49501 catctgacca aagaccatgg ctgttttctc gataacgcct gattctttca tttcccagta
    49561 aaggtcattc ccctcacgag tacgttcccc aacaccagta aatactgaaa taccaccgtg
    49621 ctcttgggca atgttgtgaa tcaattcttg gattaagacg gttttaccaa ctccggcacc
    49681 accgaaaagt ccaactttac cacctttaag gtaaggggca agaaggtcaa taaccttgat
    49741 ccctgtttca aggatttcag aagaggtaga caactcatca aaagttggag cttttttatg
    49801 aattggctga cgctctgcgt cttctgtaaa aggagcttcc aaatcaatgg tatctcccaa
    49861 aacgttgaag acacgtccca aagtttcttt acctactggt acagagattg gacgacctgt
    49921 gtccaatact tccattccac gagtcaaccc atctgttgat tccatggcga tagtacgaac
    49981 cataccatct cctaactcca aggctacttc aaggacgatt tttgtttttc tttcgtcatt
    50041 tttgtagacg acaagtgcat tgttaatctc aggaagtttt tcccctgctg caaacaaaac
    50101 gtctacaacg ggaccgataa cctgagcaat tttacctgaa ctcatctcct tctcctattc
    50161 tatataagat actgtcggtt cctagctcaa ctaggtccta agttcatttt catacgagct
    50221 ggactagagc ctattctaag gcactagcac ctgctacgat ttctgtaatt tcttgtgtaa
    50281 tcgccgcctg tctggcacgg ttatactgaa ttgtcaaatc attgatgact ttcttagcat
    50341 tatctgtcgc tgtttgcatg gctgtcatac ccgcagcatt ctcagctgtc ttggcatcga
    50401 taatggcacc gtaaatcata ctttctgcaa actgaggcaa caactgctcc agaatttctt
    50461 ctcggctggt ttccaattca aaagtcaagc tgtactcttc atccgcttca tttggatcca
    50521 agtcaacaat cggaagcatt tgttccacac gcatttgact ggttagcgta ttgacatggt
    50581 ggttgtagca aacataaagc tcatcaaaga gttcattttg gtacatttca acagtttttg
    50641 aaataatctt acgaacttga tcaaagctag gttggtctgc caagccacgt aattcataaa
    50701 gtggttgaat accgcgagcc ttaaagaaat cagctcccat cccaccgata cagatcattt
    50761 caaaaccttt accgtctggg tggtattctt ctttcaactc cataacagct ttcaaaatag
    50821 aggaattata acctccaacc aaaccgcggt ctgaagtgat aacgatatag cctgtcttct
    50881 tcacagaacg gctaatcaac atcggattag ttgaagcacc agctccatta ccatgaagga
    50941 tatctgtcaa aagtttacgc actttctgag cgtaaacttg gaagttgcga gcagcttctt
    51001 cagaacgacc tagcttagca gccgatacca tttgcatggc attagtgatt tgactcgtat
    51061 tttttgttga ggcgattttt gttttaatat catttagaga tactgccatc tgacacctct
    51121 attcttattg gaagctagat tgattgagaa actctgtaat cgcagcatcc aagactgctt
    51181 cttctggcaa gtcttttgta tcacgaatgg tttccaaaat ctctggatgt tgagcatcaa
    51241 agaaggtatg gaactcttcc tcgaaacgaa caatatcatc tactggaaca gtatccaaga
    51301 aaccatgtgt caaagcataa agaatggtta cttgtttctc aacaggtaat ggtttgtgaa
    51361 caggttgttt caagacctca acggtacgac gtccacggtt caacttagcc tgtgttgctg
    51421 cgtccaagtc agaaccaaac ttagtaaagg cttccaactc acggtatgaa gcaaggtcga
    51481 tacgaagtgt accagcaacc ttcttcatgg ctttgatttg tgcagaacca cctacacgag
    51541 atacagatga acccgcatcg atggctggac gaatacctgc attgaagagg ccatcgccaa
    51601 ggaagatttg tccatcagtg atagaaatca cgttggttgc gatataggct gagatatctc
    51661 ctgcttgtgt ctcgataaat ggtagggctg taattgatcc accaccaagt tcatcagaaa
    51721 ctttagctga gcgctcaagc aaacggctgt ggagatagaa aacatcccct gggaaggctt
    51781 cacgacctgg aggacgacga agcaagagcg atagttcacg ataagctacc gcttgttttg
    51841 atagatcatc gtatacaatc aaaacatgct taccttgata cataaattct tccgccatag
    51901 caaccccagt ataaggagct aggaagagca atggagatgg ttgtgaagca gaggctgtca
    51961 caacgattgt gtagtccaag gcaccgtact gacgaagtgt ttctacttgc gtacgaactg
    52021 ttgattcttt ttgtccaatc gcgacgtaga tacagatcat atcttgatct ttttggttca
    52081 agattgtatc aatcgcaatg gttgttttcc ctgtctgacg gtcaccgata atcaactcac
    52141 gttgaccacg accaatcggt acaagggcgt caatagcttt caaaccagtt tgcaatggtt
    52201 ctgaaacaga cttacgttgc ataacaccag gagctggtgc ttctactgga cgagttttat
    52261 cagtgtggat ttctccaaga ccgtcaactg gacgaccaag cggatccaca acacgaccaa
    52321 tcagactttc acctacaggg acttccatga ttttccctgt acggcggatt gtatcgcctt
    52381 cacggatatc tgtaaagtca cctaggatga taataccaac gtctgttgac tccaagtttt
    52441 gagccatacc ataagagccg ttttcaaaat tcaacaactc tccactcatg acattttcaa
    52501 ggccatgagc acgcgcgata ccgtccccga tataggttac aacacctgtt tcagtcacat
    52561 caaaattggg tttgaaattt tcaatttgtt gcttaattaa agcgctgatt tcttgtgcgt
    52621 taattgccaa aagaacacca ctttctattt caaattttct ttaacaactt taagttgttg
    52681 tttaatactc acatcaattg tcttgtgatt ggcaaaaatg acaaaaccac caatgagact
    52741 ttcatcgatt tgttctttta cactccttac tttcagagac atttttttct caatcaaagg
    52801 gagcaaacga gtcttctgtt catcagttag aggatgagca gacgtaatcg tcacttcaaa
    52861 tcgatttgtt tctttttcaa gtcggttcaa gcaatctaca agcacatcat aaaaaagatt
    52921 tgctctgtga ttgtaggcca gaacctggat aaagttttgt aataaaggcg acacagaatc
    52981 ttggaaaaaa gcaattgttt tttccttatc cgactcgtct actgccactt gttttaaaaa
    53041 agaaggcaga cctgtttttt caacaacttg cttgatttga gtcaagtctg aaaagatacg
    53101 gtcttcttct cctttttcaa gtaccaattg gacaaaaggc atgctgtatt tttcaattac
    53161 ctttactgtt ttcttgtcca ttaagcttct cctagctgat cgatatactg atcaatgagt
    53221 gctttatggg catgactgtc aaggttttgt gagatgattt taccagctaa gctgatggtc
    53281 aaatctgcga cctcaccctt aacactctgt aaagcttcta ctttattttg agcaatttct
    53341 tgattggctt tttcttttaa gtgtcctgct tctagtttag catctgctaa gatattagcc
    53401 ttactttgct cagctgtttc ctttgcattt tcaatgattg tcttagcttc tttacggcta
    53461 ccagccaatt catcttcgcg tttttgagcc aatacttctg ctttttgacg ggcttcttca
    53521 gctctgtcaa tatctgaagc aattttttca gctctttctt cgaaaatgcc tgtaatatta
    53581 gaccatgcaa attttttaat caagactagc aaaagaataa aagagccagt gattaaaata
    53641 aaattaccaa ttaattcacc tactgttacg tgcatcagtt actcctttct attcctcttc
    53701 aatattaatc ttattcccta aatacacaga tgtcaaaaga gtaaaaacat aagcttggat
    53761 gcaggaaata aagacagaaa atgcagtcca agccaaatta gctccaaagg ctactggata
    53821 ccaataaata gcttggtggg aaagaagaag taacaaactc gtcatgacct ctcctgcaaa
    53881 gatattccca aaaatccgca aagccaaaga taagaagttt gtaaattctt ccaagatatt
    53941 catcggtatg acaaaaacag gactcataaa agattttata ctttttttaa atccacgacg
    54001 acgaacgctt tctatatgtg tcaacaaaat gacaagaaaa gataaggtta agtcatactg
    54061 taaattagcg gttggcgaac tccaccagtt agtcccatcg atcgtttgaa gctttgtcat
    54121 taagccaagg ttattggcaa tcaccatgaa aaggaataaa caaaggaaaa agagtgagta
    54181 atctttcatg tagcgcgaac caatgttagg ttctgtaaat ccaataacaa agtcatagac
    54241 atactcaagt acattttgct ttcctttggg tttcaaggtc atattgcggc ttgcccaata
    54301 aataaagaca aaaataactc ccacaatcaa caaagtcatg gctaacatag tcagattgaa
    54361 gataacagga ccaatagaga tgattggatt aatactttct tccatctaaa atcctccctt
    54421 ttcttctcgt tccatgtatt tatttgataa taaatgagaa gacaagagtt acaaagaaag
    54481 ttccttcaat aaaggcaaca cctaaaaaca tcaaactacg aaactcagaa agcatatctg
    54541 gttggcgtgc tactgattta aacagtccat tcatcaataa accttcaccg acagatacgc
    54601 ccatacaggc aatacataag cctaaaaatg ttaaattcat aacaaattct cctttaaaat
    54661 aaaatttact tacttagtgt agtcctaaaa tttaaatttg tcaactttta accgctattg
    54721 gaagcgttta ataaaaatta tttccattt
//
LOCUS       DAVDWM010000011        48876 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVDWM010000011 DAVDWM010000000
VERSION     DAVDWM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.011"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            5..75
                     /locus_tag="VUW49_000777"
     tRNA            5..75
                     /locus_tag="VUW49_000777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VUW49_000778"
     tRNA            110..183
                     /locus_tag="VUW49_000778"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            267..356
                     /locus_tag="VUW49_000779"
     tRNA            267..356
                     /locus_tag="VUW49_000779"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:303..305,aa:Ser,seq:tga)
     gene            370..443
                     /locus_tag="VUW49_000780"
     tRNA            370..443
                     /locus_tag="VUW49_000780"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Met,seq:cat)
     gene            447..519
                     /locus_tag="VUW49_000781"
     tRNA            447..519
                     /locus_tag="VUW49_000781"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Phe,seq:gaa)
     gene            532..612
                     /locus_tag="VUW49_000782"
     tRNA            532..612
                     /locus_tag="VUW49_000782"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:566..568,aa:Tyr,seq:gta)
     gene            617..687
                     /locus_tag="VUW49_000783"
     tRNA            617..687
                     /locus_tag="VUW49_000783"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:649..651,aa:Trp,seq:cca)
     gene            702..774
                     /locus_tag="VUW49_000784"
     tRNA            702..774
                     /locus_tag="VUW49_000784"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:735..737,aa:His,seq:gtg)
     gene            785..856
                     /locus_tag="VUW49_000785"
     tRNA            785..856
                     /locus_tag="VUW49_000785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:817..819,aa:Gln,seq:ttg)
     gene            866..949
                     /locus_tag="VUW49_000786"
     tRNA            866..949
                     /locus_tag="VUW49_000786"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:900..902,aa:Leu,seq:caa)
     gene            1056..2333
                     /locus_tag="VUW49_000787"
     CDS             1056..2333
                     /locus_tag="VUW49_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET7980732.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETMIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2409..3893
                     /locus_tag="VUW49_000788"
     CDS             2409..3893
                     /locus_tag="VUW49_000788"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7980733.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDVEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQVAVEAYLGL"
     gene            3954..5234
                     /locus_tag="VUW49_000789"
     CDS             3954..5234
                     /locus_tag="VUW49_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7980734.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGIPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     DWGLPGIWAGSLLDNGFRWLFLRYRYQRYMNLKG"
     gene            5238..5858
                     /locus_tag="VUW49_000790"
     CDS             5238..5858
                     /locus_tag="VUW49_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7980735.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESRIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6024..7166
                     /locus_tag="VUW49_000791"
     CDS             6024..7166
                     /locus_tag="VUW49_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7980736.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7680..8018
                     /locus_tag="VUW49_000792"
     CDS             7680..8018
                     /locus_tag="VUW49_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET7980737.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQECLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            8029..8397
                     /locus_tag="VUW49_000793"
     CDS             8029..8397
                     /locus_tag="VUW49_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET7980738.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8504..9148
                     /gene="pcp"
                     /locus_tag="VUW49_000794"
     CDS             8504..9148
                     /gene="pcp"
                     /locus_tag="VUW49_000794"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET7980739.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTIF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9176..10033)
                     /locus_tag="VUW49_000795"
     CDS             complement(9176..10033)
                     /locus_tag="VUW49_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET7980740.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10163..11305
                     /gene="tgt"
                     /locus_tag="VUW49_000796"
     CDS             10163..11305
                     /gene="tgt"
                     /locus_tag="VUW49_000796"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7980741.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKTDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11403..13220
                     /locus_tag="VUW49_000797"
     CDS             11403..13220
                     /locus_tag="VUW49_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET7980742.1"
                     /translation="MRIKWFSLIRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13373..14524
                     /gene="nagA"
                     /locus_tag="VUW49_000798"
     CDS             13373..14524
                     /gene="nagA"
                     /locus_tag="VUW49_000798"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET7980743.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQDAA
                     KGMLNKIAIAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14687..15745
                     /locus_tag="VUW49_000799"
     CDS             14687..15745
                     /locus_tag="VUW49_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7980744.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15896..16261
                     /locus_tag="VUW49_000800"
     CDS             15896..16261
                     /locus_tag="VUW49_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET7980745.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16337..17278
                     /locus_tag="VUW49_000801"
     CDS             16337..17278
                     /locus_tag="VUW49_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET7980746.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17226..18242
                     /locus_tag="VUW49_000802"
     CDS             17226..18242
                     /locus_tag="VUW49_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7980747.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTIITQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18244..18570
                     /locus_tag="VUW49_000803"
     CDS             18244..18570
                     /locus_tag="VUW49_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7980748.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLAKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKEDGRITEEQAKAYKEYNDKNVGA
                     NRKVYD"
     gene            18563..18967
                     /locus_tag="VUW49_000804"
     CDS             18563..18967
                     /locus_tag="VUW49_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET7980749.1"
                     /translation="MIKAFTMLESLLVLGFVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18972..19232
                     /locus_tag="VUW49_000805"
     CDS             18972..19232
                     /locus_tag="VUW49_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980750.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19195..19656
                     /locus_tag="VUW49_000806"
     CDS             19195..19656
                     /locus_tag="VUW49_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET7980751.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19634..20047
                     /locus_tag="VUW49_000807"
     CDS             19634..20047
                     /locus_tag="VUW49_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET7980752.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            20184..20771
                     /locus_tag="VUW49_000808"
     CDS             20184..20771
                     /locus_tag="VUW49_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7980753.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKAEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVNSQILYSAEDLFQGNYSEFFLIVAQKSLA"
     gene            20832..21785
                     /locus_tag="VUW49_000809"
     CDS             20832..21785
                     /locus_tag="VUW49_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7980754.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMVDVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21836..23026
                     /locus_tag="VUW49_000810"
     CDS             21836..23026
                     /locus_tag="VUW49_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET7980755.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23175..23546
                     /gene="rnpA"
                     /locus_tag="VUW49_000811"
     CDS             23175..23546
                     /gene="rnpA"
                     /locus_tag="VUW49_000811"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7980756.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23521..24345
                     /locus_tag="VUW49_000812"
     CDS             23521..24345
                     /locus_tag="VUW49_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7980757.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGTTTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24364..25350
                     /locus_tag="VUW49_000813"
     CDS             24364..25350
                     /locus_tag="VUW49_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET7980758.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25401..26024
                     /locus_tag="VUW49_000814"
     CDS             25401..26024
                     /locus_tag="VUW49_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET7980759.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKVFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26155..27612
                     /locus_tag="VUW49_000815"
     CDS             26155..27612
                     /locus_tag="VUW49_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET7980760.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27635..27916
                     /locus_tag="VUW49_000816"
     CDS             27635..27916
                     /locus_tag="VUW49_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7980761.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27993..28478
                     /locus_tag="VUW49_000817"
     CDS             27993..28478
                     /locus_tag="VUW49_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7980762.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28495..29160
                     /locus_tag="VUW49_000818"
     CDS             28495..29160
                     /locus_tag="VUW49_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET7980763.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDIFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29164..30027
                     /locus_tag="VUW49_000819"
     CDS             29164..30027
                     /locus_tag="VUW49_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET7980764.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            30029..30733
                     /locus_tag="VUW49_000820"
     CDS             30029..30733
                     /locus_tag="VUW49_000820"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET7980765.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVTYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30910..32583
                     /locus_tag="VUW49_000821"
     CDS             30910..32583
                     /locus_tag="VUW49_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET7980766.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            32695..33786
                     /gene="ulaG"
                     /locus_tag="VUW49_000822"
     CDS             32695..33786
                     /gene="ulaG"
                     /locus_tag="VUW49_000822"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET7980767.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33900..35876
                     /gene="tkt"
                     /locus_tag="VUW49_000823"
     CDS             33900..35876
                     /gene="tkt"
                     /locus_tag="VUW49_000823"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7980768.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35993..36292
                     /gene="yajC"
                     /locus_tag="VUW49_000824"
     CDS             35993..36292
                     /gene="yajC"
                     /locus_tag="VUW49_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7980769.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36339..36767
                     /locus_tag="VUW49_000825"
     CDS             36339..36767
                     /locus_tag="VUW49_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET7980770.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLCQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36769..37179
                     /locus_tag="VUW49_000826"
     CDS             36769..37179
                     /locus_tag="VUW49_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980771.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37479..40130
                     /gene="adhE"
                     /locus_tag="VUW49_000827"
                     /gene_synonym="adhC"
     CDS             37479..40130
                     /gene="adhE"
                     /locus_tag="VUW49_000827"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET7980772.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(41116..41424)
                     /locus_tag="VUW49_000828"
     CDS             complement(41116..41424)
                     /locus_tag="VUW49_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7980773.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(41421..41729)
                     /locus_tag="VUW49_000829"
     CDS             complement(41421..41729)
                     /locus_tag="VUW49_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7980774.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(41734..43029)
                     /locus_tag="VUW49_000830"
     CDS             complement(41734..43029)
                     /locus_tag="VUW49_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7980775.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(43047..44456)
                     /locus_tag="VUW49_000831"
     CDS             complement(43047..44456)
                     /locus_tag="VUW49_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET7980776.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            44614..45342
                     /locus_tag="VUW49_000832"
     CDS             44614..45342
                     /locus_tag="VUW49_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7980777.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            45801..45995
                     /locus_tag="VUW49_000833"
     CDS             45801..45995
                     /locus_tag="VUW49_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7980778.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(46361..47671)
                     /locus_tag="VUW49_000834"
     CDS             complement(46361..47671)
                     /locus_tag="VUW49_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7980779.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGKTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            47908..48780
                     /locus_tag="VUW49_000835"
     CDS             47908..48780
                     /locus_tag="VUW49_000835"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET7980780.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFKRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALDDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDI"
BASE COUNT        14888 a         8716 c        10711 g        14561 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaaac tattttggag gattacccaa gtccggctga agggaacggt
      301 cttgaaaacc gtcaggcgtg taaaagcgtg cgtgggttcg aatcccacat cctcctttta
      361 tattattaac gcgggatgga gcagctcggt agctcgtcgg gctcataacc cgaaggtcgt
      421 aggttcaaat cctgctcccg caataaggct cggtagctca gttggtagag caatggattg
      481 aagctccatg tgtcggcggt tcgattccgt ctcgcgccat ttatatattt tggaagggta
      541 gcgaagaggc taaacgcggc ggactgtaaa tccgctcctt cgggttcggg ggttcgaatc
      601 cctccccttc cattttacgg gcatagttta aaggtagaac taaggtctcc aaaaccttca
      661 gtgtgggttc aattcctact gcccgtgtta atagaattat ggcgggtgtg gtgaagtggt
      721 taacacacca gattgtggct ctggcatgcg tgggttcgat ccccatcact cgcctatttt
      781 atattggggt atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg
      841 aatccagcta ccccagttac tatttgccgg cgtggcggaa ttggcagacg cgctggactc
      901 aaaatccagt gtccgcaagg acgtgccggt tcgaccccgg ccgccggtat agtatagtgt
      961 taggaacgtt gttattcttc gttccttttt tatattattt ttggtataat tatagttatt
     1021 caaattttat ttagattgag aaagtgtagg gtagtatgtc ttgttctatc gatttattaa
     1081 aacatcggta tttgaaaaat attaaagaaa atcctgaatt gtttgtcgga attgagttgg
     1141 agtatcctgt tgcaagttta gaaggtgatg ctacagatgt tgaagttata aaggatctat
     1201 ttcattattt agtttctact ttggatctca cggtagcaaa ggtagatgat tttggcaatc
     1261 tgatccagtt agtagatccg ataagtcagg atgctatttt atttgaagtt tcctatacaa
     1321 cgattgagtt tgcatttggt aaggctgaaa cgattcaaga ggtcgaaaat cgtttcaata
     1381 attatatgaa tgtaattcag agaaagttag ctgaatcaaa tcatgctatt gttggctgtg
     1441 gtatccatcc caactgggat aaaaatgaga attgtccagt ggcttatcca cgctatcaga
     1501 tgttgatgga ttatttgaat ttgagtagaa atattattaa atcagattta catcatttcc
     1561 ctgaatatgg tacttttatc tgtgggagcc aggttcagct ggatatttca aaaaccaact
     1621 acttacgggt gattaatgct tttactcaaa ttgaagcggc taaggcttat ttatttgcaa
     1681 actctgagtt ttcgggtgcg gattgggata cgaaaatttc aagggatatt ttctgggaag
     1741 aatctatgca tggtatctat ccagagaatg ttggggtcaa tgctagactc cttaatgatg
     1801 aagctgattt ttttgactat ctaaatcatt ctgcgatttt tactgcggaa cgtgatgggc
     1861 agacctatta tttttatcct attcaagctg gggactattt ggctacgcct gaaatccaag
     1921 catttgctct gaatggggat gaggttatta tttaccccca agagaaggat tttgaaactc
     1981 atcgtagtta ccagtaccaa gacttaacga ctcgaggaac agttgagttt cgtagtgtgt
     2041 gtacacagcc acttgatagg acttttgctt ctgcagcttt tcacttggga ttattggtta
     2101 atttagacaa gttagaagct tacttagaaa cagcaccttt ctttaaagta tttggttatg
     2161 attacaagtt tttaaggaga caattttcta agaaaaatct tacagatgag gaagaaacta
     2221 tgattattga attttccaaa gacttactcc tactagctga ggagggacta gtggtgagaa
     2281 ataaggaaga aatgacctat ttacagcctt tgagagaaga attgagccta taatttctct
     2341 tataaaggga gaattttctg aaaaatcatg atataatgga cgagactata gataaaggat
     2401 agagagtaat gacattagtt tatcaatcaa cgcgtgatgc caacaataca gtaactgcca
     2461 gccaagcaat tttgcaaggt ttggcgacgg acggcggttt gtttacaccg gttacttatc
     2521 caaaggtaga tttgaacttt gacaaattga aagatgcttc ttaccaggaa gttgctaagc
     2581 tagttttgtc agcattttta gatgacttta cagctgagga gttggactac tgtatcaaca
     2641 atgcctacga tagcaaattt gatactccag ctattgcacc attagtgaaa ttagatgggc
     2701 aatacaattt ggaacttttc catggttcaa cgattgcctt taaggatatg gccttgtcta
     2761 ttttgccata ctttatgacg actgctgcta agaaacatgg tttggagaac aagattgtta
     2821 tcttgacagc gacatctggt gacacgggga aagctgctat ggcggggttt gcggatgtgc
     2881 ctggtactga gattatcgtc ttttatccaa agggtggtgt cagcaaggta caagagttgc
     2941 aaatgaccac tcagaatggt gacaatactc atgttatcgc tatagatggt aactttgacg
     3001 atgcgcaaac caacgtgaag cacatgttta acgacgtggc tcttcgtgaa agattagcgg
     3061 ccaacaagtt gcaattttca tctgctaact ctatgaatat cggtcgtttg gtgccacaga
     3121 ttgtttatta tgtttatgcc tatgctcagt tggtcaagac tggtgagatc gtggctggtg
     3181 ataagattaa cttcacagta ccaacaggaa actttggaaa tatcttggct gccttttatg
     3241 ccaaacaaat cggtctccca gttggtaaat tgatctgtgc ttcaaatgat aacaatgttt
     3301 tgacagactt cttcaagaca cgtgtttacg acaagaaacg tgagtttaag gtaacaacca
     3361 gcccatctat ggatatcttg gtatcttcaa acttggagcg tttgattttc catcttttgg
     3421 ggaatgatgc ggttaagacg gctgaactca tgaatgcctt gaatacacaa ggacaatatg
     3481 aactgactga ttttgatgta gagattctgg agctctttgc agctgaatat gcgactgagg
     3541 aagaaacagc ggcagaaatc aagcgtgttt atgagttaga ttcttatatt gaggatccac
     3601 atacggcggt tgcctcagca gtttatagaa aataccaagc ggttactgat gatgcgacta
     3661 agacagtgat tgcttcaaca gctagtccct acaagttccc agtagttgca gtagaagctg
     3721 taactggaaa agcaggttta acagactttg aagctttggc tcaattacat gaaatctcag
     3781 gcgttgcagt gccaccagca gttgatgggc ttgagacagc tcctgttcgt cacaagacaa
     3841 cagtggcagc tgctgacatg caagtagcgg ttgaggctta tttaggactt taagatagag
     3901 ggagtaaact cggttgggaa accaactgag tttcttttca tcaggaggag agattgttta
     3961 agaaaaataa agacattctt aatattgcat tgccagctat gggtgaaaac tttttgcaga
     4021 tgctaatggg aatggtggac agttatttgg ttgctcattt aggattgata gctatttcag
     4081 gggtttcagt agctggtaat attatcacca tttatcaggc gattttcatc gctctgggag
     4141 ctgctatttc cagtgttatt tcaaaaagca tagggcagaa agaccagtcg aagttggcct
     4201 atcatgtgac tgaggcgttg aagattacct tactattaag tttcctttta ggatttttgt
     4261 ccatcttcgc tgggaaagag atgataggac ttttggggac ggagagggat gtagctgaga
     4321 gtggtggact gtatctatct ttggtaggcg gatcgattgt tctcttaggt ttaatgacta
     4381 gtctgggagc cttgattcgt gcaacgcata atccacgtct gcctctctat gttagttttt
     4441 tatccaatgc cttgaatatt cttttttcaa gtctagctat ttttgttctg gatatgggga
     4501 tagctggtgt tgcttggggg acaattctgt ctcgtttggt tggtcttgtg attttgtggt
     4561 cacaattaaa actgccttat gggaagccaa cttttggttt agataaggaa ctgttgacct
     4621 tggctttacc agcagctgga gagcgactta tgatgagggc tggagatgta gtgatcattg
     4681 ccttggtcgt ttcttttggg acggaggcag ttgctgggaa tgcaatcgga gaagtcttga
     4741 cccagtttaa ctatatgcct gcctttggcg tcgctacggc aacggtcatg ctgttggccc
     4801 gagcagttgg agaggatgat tggaaaagag ttgctagttt gagtaaacaa accttttggc
     4861 tttctctgtt cctcatgttg cccctgtcct ttagtatata tgtcttgggt gtaccattaa
     4921 ctcatctcta tacgactgat tctctagcgg tggaggctag tgttctagtg acactgtttt
     4981 cactacttgg gatccctatg acgacaggaa cagtcatcta tacggcagtc tggcagggat
     5041 taggaaatgc acgcctccct ttttatgcga caagtatagg catgtggtgt atccgcattg
     5101 ggacaggata tctgatgggg attgtgcttg attggggctt gcctggtatt tgggcagggt
     5161 ctctcttgga taatggtttt cgctggttat ttctacgcta tcgttaccag cgctatatga
     5221 acttgaaagg ataggaaatg caaaaaacag cttttatttg ggatttagac gggactttat
     5281 tggactctta cgaagcgatt ttatcaggga ttgaggagac ttttgctcag ttttctattc
     5341 cttatgataa ggagaaggtg agagagttta tcttcaagta ttcggtgcaa gatttgcttg
     5401 tgcgggtggc agaagataga aatctggatg ttgaggtgct aaatcaggtg cgtgcccaga
     5461 gtctggctga gaagaatgct caggtagttt tgatgccagg tgcgcgtgag gtgctagctt
     5521 gggcagacga atcaagaatt cagcagttta tatatactca taaggggaac aacgctttta
     5581 ccattctcaa ggacttgggg gtggaatcct attttacaga gattttaacc agtcagagtg
     5641 gctttgtgcg gaagccaagt ccagaagcgg ctacctatct gctagataag tatcagttga
     5701 attctgataa tacttattat ataggggatc ggactctgga tgtggaattt gcccagaata
     5761 gtgggattca aagtatcaac tttttagagt ctacttatga agggaatcac aggattcaag
     5821 cgttagcaga tatttcccgt atttttgaga ctaagtgata aaaagattgt gtcagttttg
     5881 tgacagagac ctaacaaact atttcaagta acctagtttg ttacaaggaa tagacagttc
     5941 tgttaaatag gcccgagagg gctttttttc tacatttttt gtgttatgat agacaggtac
     6001 tcatttgaaa ggaatttgaa agaatgaaga aaagaatgtt attagcgtca acagtagcct
     6061 tgtcatttgc cccagtattg gcaactcaag cagaagaagt tctttggact gcacgtagtg
     6121 ttgagcaaat ccaaaacgat ttgactaaaa cggacaacaa aacaagttat accgtacagt
     6181 atggtgatac tttgagcacc attgcagaag ccttgggtgt agatgtcaca gtgcttgcga
     6241 atctgaacaa aatcactaat atggacttga ttttcccaga aactgttttg acaacgactg
     6301 tcaatgaagc agaagaagta acagaagttg aaatccaaac acctcaagca gactctagtg
     6361 aagaagtgac aactgcgaca gcagatttga ccactaatca agtgaccgtt gatgatcaaa
     6421 ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagc tccaaaagaa gtagcatcaa
     6481 gttcagaagt tacaaagaca gtgattgctt ctgaagaagt ggcaccatct acgggcactt
     6541 ctgtcccaga ggagcaaacg gccgaaacaa ctcgcccagt tgaagaagca actcctcagg
     6601 aaacgactcc agctgagaag caggaaacac aagcaagccc tcaagctgca tcagcagtgg
     6661 aagtaactac aacaagttca gaagcaaaag aagtagcatc atcaaatgga gctacagcag
     6721 cagtttctac ttatcaacca gaagagacga aaataatttc aacaacttac gaggctccag
     6781 ctgcgcccga ttatgctgga cttgcagtag caaaatctga aaatgcaggt cttcaaccac
     6841 aaacagctgc ctttaaagaa gaaattgcta acttgtttgg cattacatcc tttagtggtt
     6901 atcgtccagg agacagtgga gatcacggaa aaggtttggc tatcgatttt atggtaccag
     6961 aacgttcaga attaggggat aagattgcgg aatatgctat tcaaaatatg gccagccgtg
     7021 gcattagtta catcatctgg aaacaacgtt tctatgctcc attcgatagc aaatatgggc
     7081 cagctaacac ttggaaccca atgccagacc gtggtagtgt gacagaaaat cactatgatc
     7141 acgttcacgt ttcaatgaat ggataaaccc gacttgataa catcattttg acgaatgaga
     7201 tctagctttc gtgatggaaa gcgattctcg ttcgtttttt ctttgtcata ctcttcgaaa
     7261 atctcttcaa accacgtcag ttttatctga aacctcaaag ctgtgctttg agcaacctgc
     7321 gactagcttc ctagtttgct ttttgatttt cattgagtat caatttgaat ggaaaatgga
     7381 aagttatcat cttgtaatga gttaagcaac attcttgcaa tctattttat agtagatgga
     7441 aactagaata gtacacaact gcttctaaaa cattgttaga aatcgatttg actgtcctga
     7501 tcgatttgtc ctgctcttat ttcatttcac tatactttat attataattg actggtcaac
     7561 tattgataaa tcaataaaaa gagaggggaa gaaatgctag agattcaaga tttactgtat
     7621 caactccgct tgtctgagca agcgagtacg caattgtttg aaaaaaggct tggattagtt
     7681 tgacacggta tcagatttta ctgtttttgc tggagcattc tccttgtaac caaatggcgg
     7741 ttcaggagtg tttgaaaatt gatcaggctg ctttgacacg gcatttcaag attttggaaa
     7801 cggaaggttt ggtggagcgt catcgtaatc ctgaaaatca gcgggaagtg ttggtagagg
     7861 ctgcgaagta tgccaaggag cagttagtgg tgaatccccc tctgcaacat atcaaggtta
     7921 aggaagagat ggaaagtatc ttaacagagt ttgagagaac agaactcagc cgtttattaa
     7981 ataaattggt tttgggtatt gaaaatatag aaatttaagg agaaatagat gtcaattatt
     8041 acaattattt taacaacgat cgttgctttg gagcattttt acatttttta tttggaaagt
     8101 attgccacgc aatcagatgc gactagtcgt gtatttaata tggaaaagga agaattggct
     8161 catccgtcag taagttcatt gttcaaaaat caagggattt ataaggctct gctaggagtc
     8221 tttctcttgt atggcattta tttctcacag aatttagaaa ttgtgactat ttttgtctta
     8281 tttgtgattg gtgctgcgac ttacggctct ttaacagcgg ataaaaaaat tattttgaag
     8341 caaggtggat cagctatttt ggccttgatt agtattttac tctttaaata cacttgaagg
     8401 tcgattctaa tctcgctaat ccttttcaat ccagaataag ggaaatatgt tatacttgtt
     8461 tttaagaaaa aagtctcatt gaattggttt tgaggagtta gaaatgaaag tattagtgac
     8521 aggttttgag ccctttggag gggaaaaggg caatccagct ttggaggcca ttaaaggttt
     8581 accagctgaa atccatggtg ctgaggtccg ttggctagag gtgccgacaa tttttcacaa
     8641 atctgctcaa gtattggaag aagagatgaa tcgttatcaa cctgactttg ttctttgtat
     8701 tgggcaagct ggtggaagaa ctagtttgac acctgaacga gtggccatta atcaagacga
     8761 tgcacgcatt tctgataacg aagataatca accgattgac cgtcccattc gcccagatgg
     8821 tgcttcggcc tactttagta gtttgccgat taaagcgatg gttcaagcta taaaaaaaga
     8881 gggcttaccg gcctctgttt ccaatacggc agggactttt gtctgcagcc atttgatgta
     8941 tcaggctctc tatttggtag aaaagaaatt tccatatgtt aaggcaggtt ttatgcatat
     9001 tccttatatg atggaacagg tggtgaacag accgactact ccagctatga gtttagtgga
     9061 tattcggcga gggatagaag cagcaatcgg cgctatgata gaacatggag atcaggaact
     9121 caagttggta ggcggagaaa ctcattgata gaaaaaagct tgaggggaaa aaccttcaag
     9181 cttttggacg ttttcgagcc aatactgctc ggtaaaacat aattttagtg cattggatat
     9241 aaggtaggag tgaaaaacta gcaatgccaa aggtaatcca attgaggaag taccaaggaa
     9301 gaagctgtaa atctaggaca aagcgctgga acttgtagcc cttcataaag gaacggctag
     9361 tttttaggat tcgtcttggt gggacctgtc ctaggtctag actataacag agaagaaatt
     9421 ccacctgtga ataggcataa tactgtggaa tatagaggat atttcctaca atgatcaaga
     9481 tgagacttgc aagaaagtag agtccaaaga ccatgaggaa acgctcggtt tcaactgatg
     9541 agagatctag atttggaaac tcaggatgta gggtgacgaa ttttttggct aaaaagctac
     9601 tataaaagag gaggtaaatc ccaagtaaat tagggatact ccataaaaag agatagaaac
     9661 gtttgagaag tagggtcaaa aaggtttgag aaaagcgctc ctcatcaaag agagctaggc
     9721 tgttttttac agatggctcc gttttagaat ctttcatgag tgtcagtgtt gcatagacgg
     9781 aactggtcaa aagaatagtc ccgataaagg agactagtag aggaaagagg taggcttgaa
     9841 gtatttggcc aagtatgctg aaaaatggct gttctaaaac agtcccgtgg atccgagata
     9901 agggattaag aaaaccagat aagatgacca gcatactggg aaggatatag aggagaaaga
     9961 gacggggggt gtcagcctga aaatgttttg actcctgacg aattgttttt aaatcaattt
    10021 ttggatagtt cattctctta ttataccata gttcttatac atagttcgtg acagttccta
    10081 ctttttttga taaaatcata cagtgtgtcc ttgggcacac tgtatgaact gggactgtct
    10141 ttcccagctt cggaggtaaa aaatgtcaga ttcaccaatc aaatatcgtt tgattaagaa
    10201 agaaaaacac acaggagctc gtctaggaga aatcatcact ccccacggta cctttccgac
    10261 acctatgttt atgccagttg ggacacaagc cactgtcaaa actcagtcac ctgaagaatt
    10321 gaaggagatg ggttcgggaa ttatcctatc aaacacctat catctctggc ttcgccctgg
    10381 agatgaactc attgcacgcg ctggtggtct ccacaagttc atgaattggg accagcctat
    10441 cttgacagat agtggtggtt ttcaggttta ttctttagca gatagccgta atatcacaga
    10501 agaaggagta acctttaaaa atcatctaaa tggttctaag atgttcctat ccccagaaaa
    10561 agccatctct attcagaata atctgggttc agacatcatg atgtcctttg atgaatgtcc
    10621 tcagttttat caaccttatg actacgttaa gaaatcgatc gagcgtacca gccgttgggc
    10681 tgagcgtggt ttgaaggctc accgtcgtcc acatgaccaa ggtttgtttg gaattgtgca
    10741 aggtgcagga tttgaagacc ttcgccgcca atcagctcat gatcttgtca gcatggattt
    10801 ctcaggctac tctatcggtg gtttggcagt gggagaaacc catgaagaga tgaatgcggt
    10861 cttggacttt acaactcaac tgctgcctga aaataaacct cgttatctga tgggtgtggg
    10921 agcgccagat agcttgatcg atggggtcat tcgtggggtg gatatgtttg actgtgtctt
    10981 accgactcga attgctcgta acgggacttg tatgaccagt caaggacgtt tggttgtgaa
    11041 aaatgcccag tttgctgagg actttacgcc actggatcct gagtgtgatt gctacacatg
    11101 taataactat acacgcgctt accttcgtca cctgctcaag actgatgaaa cctttggtat
    11161 ccgcttgact agctaccaca atctttactt cttgcttaac ctgatgaagc aagtgcgaca
    11221 agccatcatg gatgacaatc tcttggaatt ccgtgagtat tttgtggaaa aatatggcta
    11281 taataagtca ggacgtaatt tctaaaatgg aattgatata aaaaaatcct aagttttctc
    11341 ttaggatttt tcttcttttt ttgatagaat aaagtgtaca atgaaaggaa gaataaactc
    11401 gtatgcgcat taaatggttt tccttgatta ggattacagg tttacttttg gtactcttgt
    11461 atcacttctt tcagacgatc tttcctggag gatttttcgg ggtagatgtc tttttcacat
    11521 tttcaggttt cctgattaca gctctactca ttgaagaatt ttctaaaaac catgagattg
    11581 atttgatagg attttttaga agacgctttt atcggattgt gccacctgtg gttttgatgg
    11641 tcttggtgac catgcctttt actttcttgg ttcgccaaga ctatgttgct ggaattggtg
    11701 gccagattgc gggcgtctta ggctttatga ccaacttcta tgaactccta acaggtggga
    11761 gttatgaatc tcagttcatt cctcatttgt ttgttcataa ttggagtttg gcagttgagg
    11821 ttcattacta tattctttgg ggattggcag tttggttctt atccaaacaa gctaaatcaa
    11881 atggtcagtt gaaggggatg gtctttctct tatctgctgt tgccttcttg atcagtttct
    11941 tctccatgtt tattggtagt tttctagtga cctcttattc ctctgtttat ttctccagtt
    12001 taactcatgt ctatccattc tttttgggaa gtatgttagc aactattgta ggcgttcgtc
    12061 agacgacttc cctcgtcaag cagttggata aaatctggga tttacgaaag actttggtag
    12121 tttttggagg aggctttggt ttcctagttc ttttgacttt ctttgtcaaa ttcacttatc
    12181 tttttgccta tcttatcggc ttcttacttg ccagtcttgc agctcttgcc atgattctgg
    12241 cggcgcgtgt cttacatgaa aagacacatc atatacagga gccgaagatt atcagctttt
    12301 tagcggatac tagctatgcg gtttatcttt tccattggcc tttctatatc attttctcac
    12361 agttgacatc aaatcttctt gctgtattac tgactctgat ttgttcttat ggctttgcca
    12421 gtctgtcatt ctatgtattg gaaccttgga ttgcaggtaa gaacacacct attgtccaaa
    12481 cccttcgtcc cctgccttat attcacgcaa ttcttgcagc aggtacagga atcttgacca
    12541 tcattgtctg cacggtgacc ttgttggcac cacaagtggg agcgtttgag acagacttga
    12601 ctgtcaatgg cttgaagcaa gctgcaacaa atattggcca gaccaaggtg atggcagaac
    12661 gggcagatgc aaacagtttg ggaattgctg atggcactat gttaattggt gactcagtgg
    12721 ctttaagggc aaatacagca ctacagacag ctcttcctgg agcacagatt aacgcgcagg
    12781 tcagcgtaac aaccaagacc gcaaatgaaa tcatgctaaa taatagccag aataaatttt
    12841 tacctaagac ggtggtcatt gcgactgggg taaataatcc tgagaattac aaggatgact
    12901 gggacagtat cgtgaaaaat cttcctaagg gacaccatat gattttggtg actccttatg
    12961 agggagataa gacaaaagag acctatgcca tcgttgagaa ggctgctgcc tatatgagag
    13021 aattggcaga gaagacacct tacattacga tagcagattg gaatcaagtt gcgaaagagc
    13081 atccagaaat ttgggctgga acagaccagg ttcatttcgg gagtgagagt agcactatcg
    13141 aagcaggagc aaaattgtat gcagatacga ttgccacagc tttgcagaca gctcaagaca
    13201 agccggttaa atcaaaataa cttgtattaa aaaagagaag agttggagaa atccagctct
    13261 tttaaaatat ctccaaaaaa taggtctata ccatttacaa ataaaaaaga aaggtttata
    13321 atgtaattga cataataaat tgtagaatca atcttttaag gaggttaaca ttatgcctaa
    13381 ctatattaaa gcggatcagt ttttctaccc acacggagtt cgtcgaggtg gttacttgga
    13441 acttgtggac ggcaagtttg ggaaacatgt agagcagatt cctgaagggg ctgaggtgat
    13501 tgactataca ggttatagca ttgccccagg tcttgtggat actcatattc atggatatgc
    13561 aggtgtagat gtgatggaca acaacattga aggtacattg catactatga gtgaaggact
    13621 tcttagtacc ggtgttacca gtttcttacc cacaacttta acagccactt atgagcaatt
    13681 gcttgcagtc actgaaaatc ttggaaacca ttataaagaa gcaacaggtg ctaagattcg
    13741 tgggatttat tatgaaggtc catatttcac agaaactttt aagggggcac aaaatccaac
    13801 ttatatgaga gacccgggtg ttgaggagtt tcattcttgg caggatgctg caaaagggat
    13861 gctaaataaa atcgctattg ccccagaacg tgatggggtg gaagactttg ttcgtacagt
    13921 tacgggcgaa ggtgtgacgg ttgctcttgg acattcaaac gcgacttttg atgaagccaa
    13981 aaaagcagtc gatgctggag cgagtgtttg ggtgcatgcc tacaatggaa tgcgtgggtt
    14041 gactcaccgt gaattgggta tggttggagc catgtaccaa ttgccacata cctatgcaga
    14101 gttgatctgt gatggtcacc acgtagatcc aaaggcttgc gaaattctta tcaaacaaaa
    14161 aggaacagaa aacatcgctc ttatcacaga ctgtatgaca gctgggggat tggaagacgg
    14221 agattatatg ttgggagaat tcccagttgt cgttgcaaat ggaactgcac gcctcaaatc
    14281 gacaggtaac ttggcaggtt ctatcctcaa actcaaagat ggtttgaaga atgtggtcga
    14341 atggggaatt gcgaatccgc atgaagcagt catgatggcc agcttcaacc cagctaaatc
    14401 cgttcacatc gatgacgtct gtggccaaat ccgtgaaggc tacgacgctg acttcatcgt
    14461 attagataaa gatttggaat tggtagcaac ctacctagat ggtgtgaaac gttatcaagc
    14521 ataagagaga aggcagaagt tagagactag cttctgcttt tttatacagg gtactttact
    14581 ggtaaataaa aagttaaaaa aatcacaaaa aaagcttgaa gaaacaaaga aaaaggctta
    14641 caattgaact ataaatatta caaataaaaa aacggaggag tgctttatga aagcctatac
    14701 ttatgttaaa ccaggacttg cttcttttgt tgatgtagac aaaccagtta ttcgcaagcc
    14761 aacagacgct attgtgcgta ttgtaaaaac cactatttgt ggaacagacc tccatattat
    14821 caaaggggat gttcctactt gccaaagtgg taccattctt ggccacgaag ggattgggat
    14881 tgttgaagaa gttggggaag gagtttccaa cttcaaaaaa ggtgacaagg tcttgatttc
    14941 ttgcgtctgt gcctgtggta aatgctacta ctgtaaaaaa ggaatttatg cccactgtga
    15001 agacgaaggg ggctggattt tcggtcactt gattgatggt atgcaggctg aatatctacg
    15061 tgtccctcat gcagataata ctctttacca tactccagaa gacttgtcag atgaagcttt
    15121 ggttatgctg tcagacattc tgcctactgg atatgaaatt ggtgtcttaa aagggaaagt
    15181 agaacctggt tgcagcgtag ccattattgg ttcaggtcca gttggattgg ctgctctttt
    15241 aacagcccaa ttctattcac cagctaaatt gattatggta gacctagacg ataaccgctt
    15301 ggaaactgcc ctatcattcg gtgcgactca taaggttaat tcttcagacc ctaaaaaagc
    15361 cattaaagaa atttatgatt tgacagatgg tcgtggtgtg gatgtcgcta tcgaagctgt
    15421 agggattcct gaaacatttg atttctgtca aaagattatc ggtgtagacg gaacggttgc
    15481 caactgtggt gtgcatggta aaccagttga attcgattta gataaacttt ggattcgcaa
    15541 catcaatgta acaactggtt tggtatctac aaatacgact ccacaattgt tgaaagcact
    15601 tgaaagtcat aagattgaac cggaaaaatt ggtaactcac tatttcaaac tcagtgaaat
    15661 tgaaaaagcc tacgaagtct tcagtaaggc agcagaccac catgccatta aggtcattat
    15721 cgaaaacgat atctcagaag cctaagtagt aaaaatattt ttgtacataa gcaaatagaa
    15781 attcagtcat ccatcagatg gctggatttt ttatcaaaaa attaagaaat gagcatattt
    15841 ctttccttgt ctggcggaat tggttataat atacggtaca aaggaatgaa tgaatatgta
    15901 tcgtgttata gaaatgtacg gagattttga accgtggtgg ttcttagaag gttgggaaga
    15961 agatattgta gcaagtagaa aatttgacca gtattatgat gctctcaaat actacaaaac
    16021 ttgctggttt agattggaac aagaatcccc tctttataaa agtagaagcg acttgatgac
    16081 cattttttgg gacccggaag accaacgctg gtgtgatgaa tgtgatgagt atttacaaca
    16141 ataccattct ttggctcttt tgcaggatga gcaggttatc ccagacgaaa aactacgctc
    16201 aggctatgaa aaacaaacca gtcaggaaag gaatcgttct tgccgtatga aattaaaata
    16261 gagaaaagta acttttttgg agttgctttt tttatttttc taacgctttg cgaatagtat
    16321 aggtgaggag gtaagtatgg ttcaagaaat tgcacaagaa atcattcgtt cggctcggaa
    16381 aaaaggggcg caagacattt attttgtccc taagttagat gcctatgagc ttcatatgag
    16441 ggtaggagac gagcgctgta aaattggtag ctatgatttt gaaaagtttg cagccgttat
    16501 cagtcacttt aagtttgtgg cgggtatgaa tgtgggagaa aaaagacgta gtcaactggg
    16561 ttcctgtgat tatgcctatg accagaagat agcgtctcta cgtttatcta ctgtaggcga
    16621 ttatcggggg catgagagtt tggttatccg tttgttgcac gatgaggagc aggacttgca
    16681 tttttggttt caggatattg aagaattagg caagcagtac aggcaacggg gactctatct
    16741 ttttgctggt ccggttggga gtggtaagac gaccttgatg catgaattgt ccaagtcact
    16801 ctttaaagga cagcaagtta tgtccatcga agatcctgtc gaaatcaagc aggacgacat
    16861 gcttcagttg cagttgaacg aagcaatcgg cctaacctat gaaaatctaa tcaaactttc
    16921 cttgcgtcat cgaccagatc tcttgattat cggagaaatt cgtgacagcg agacggcgcg
    16981 tgcagtggtc agagctagtt tgacaggtgc gacagtcttt tcaaccattc acgccaagag
    17041 tatccgaggt gtttatgagc gtctgctgga gttgggtgtg agtgaagaag aattggcagt
    17101 tgttctgcaa ggagtctgct accagagatt aatcggggga ggaggaatcg ttgactttgc
    17161 aaacagagat tatcaagaac accaagcagc caagtggaat gagcaaattg accagcttct
    17221 taaagatgga catatcacaa gtcttcaggc tgagacggaa aaaattagct acagctaagc
    17281 aaaaaaatat catcacccta tttaacaatc tcttttctag cggttttcat ctggtggaga
    17341 ctatctcctt tttagatagg agtgctttgt tggacaagca gtgtgtgatc cagatgcgtg
    17401 cgggcttgtc tcaagggaaa tcattctcag aaatgatgga aagtttggga ttttcaagca
    17461 ccattatcac tcagttatcc ctagctgaag ttcatggaaa tctccacctg agtttgggaa
    17521 agatagaaga atatctggac aatctggcta aggtcaagaa aaaattaatt gaagtagcga
    17581 cctatccttt gattttgctg ttttttcttc tcttaattat gctggggcta cggaattacc
    17641 tgctcccaca actggatagt agcaatattg ccacccgaat tatcggtaat ctgccacaaa
    17701 tttttctagg catggtaggg cttgtttccg tgcttgccct tttagcacta actttttata
    17761 aaagaagttc taagatgagt gttttttcta tcttagcacg ccttcccttt attggaatct
    17821 ttgtgcagac ctacttgaca gcctattatg cacgtgaatg ggggaatatg atttcacagg
    17881 gaatggagct gacgcagatt tttcaaatga tgcaggaaca aggttcccag ctctttaaag
    17941 aaatcggtca agatctggct caaaccctga aaaatggccg tgaattttct cagacgatag
    18001 gaacctatcc tttctttagg aaggaattga gtctcatcat agagtatggg gaagttaagt
    18061 ccaagctggg tagtgagttg gaaatctatg ctgaaaaaac ttgggaagcc ttttttaccc
    18121 gagtcaaccg caccatgaat ttggtgcagc cactggtttt tatctttgtg gcactgatta
    18181 tcgttttact ttatgcggca atgctcatgc ccatgtatca aaatatggag gtaaattttt
    18241 aaaatgaaaa aaatgatgac attcttgaaa aaagctaagg ttaaagcttt tacattggtg
    18301 gaaatgttgg tggtcttgct gattatcagc gtgcttttct tgctctttgt acctaatctg
    18361 gccaagcaaa aagaagcagt caatgacaaa ggaaaagcag ctgttgttaa ggtggtggaa
    18421 agccaggcag aactttatag cttggaaaag aatgaagatg ctagcctaag caagttaaaa
    18481 gaagatggac gcatcacgga agaacaggct aaagcttata aagaatacaa tgataaaaat
    18541 gtaggagcaa atcgtaaagt ctatgattaa ggcctttacc atgctggaaa gtctcttggt
    18601 tttgggtttt gtgagtatcc ttgccttggg cttgtccggc tctgttcagt ccacttttgc
    18661 ggcggtagag gaacagattt tctttatgga gtttgaagaa ctctatcggg aaacccaaaa
    18721 acgcagtgta gccagtcagc aaaagactag tctgaactta gatgggcaga cgcttagcaa
    18781 tggcagtcaa aagttgccag tccctaaagg aattcaggcc ccatcaggtc aaagtattac
    18841 atttgaccgt gctgggggca attcgtccct ggctaaggtt gaatttcaga ccagtaaagg
    18901 agcgattcgc tatcaattat atctaggaaa tggaaaaatt aaacgcatta aggaaacaaa
    18961 aaattagggc agtgatttta ctggaagcag tagtcgctct agctatcttt gccagcattg
    19021 cgaccctcct tttgggacaa attcaaaaaa ataggcaaga ggaagcaaaa atcttgcaaa
    19081 aggaagaagt cttgagggta gctaagatgg ccttgcagac aggtcaaaat caggtaaaca
    19141 taaacggagt tgagattcag gtgttttcta gcgaaaaggg attggaggtc taccatggtt
    19201 cagaacagtt gttggcaatc aaagagccat aaggtcaagg cttttacctt gttagaatcc
    19261 ctgcttgccc tgctcgtcat cagtggaggc ttactccttt ttcaagctat gagtcagctc
    19321 ctcatttcag aagttcgcta ccagcaacaa agcgagcaaa aggagtggct cttgtttgtg
    19381 gatcaacttg aggcagaatt agaccgttcg cagttcgaaa aagtagaaag caatcgccta
    19441 tacatgaagc aagatggcaa ggacatcgcc atcggtaagt caaagtcaga cgattttcgt
    19501 aaaaccgatg ctagtggacg ggggtatcag cctatggttt atggcctcaa atcagctcag
    19561 attacagagg acaatcaact ggttcatttt cgtttccagt ttcaaaaagg cttagaaagg
    19621 gagttcatct atcgtgtgga aaaagaagaa agttaaggca ggtgttctcc tctatgcagt
    19681 caccatggca gctatcttta gtcttttgtt gcaattttac ttgaaccgac aagtcgccca
    19741 ctatcaagac tatgctttga ataaagaaaa attggttgct tttgccatgg ctaaacgaac
    19801 caaggataaa gccgagcaag aaagtgggga acaggtcttt aatctaggtc aggtaagcta
    19861 tcaaaacaag aaaacaagct tggtgacgac tgttcgtacg gataagagtc aatatgagtt
    19921 tctgtttcct tcagtcaaaa tcaaagaaga gaaaagagat aaaaaggaag aggtagcgac
    19981 tgattcaagc gaaaaagcgg agaagaaaaa atcagaagag aagcctgaaa agaaagagaa
    20041 ttcctagtca attcaactat aatgcgttga atccagaata gtccactgta gtttctagaa
    20101 aattgctgga aatggatgtt aagctccaat tcatttgttt atatcttatt tcagtttact
    20161 atactttgtg ctaaactaaa gatatgaaac atgattttaa ccacaaagca gaaactttcg
    20221 attcccctaa aaatatcttc ctcgcaaact tggtatgtca agcagtcgag aaacagattg
    20281 atattttatc agacaaagaa attttagatt tcggtggtgg cacgggtcta ttagccttgc
    20341 ccctagccaa gcaggctaag tcagttactc ttgtagacat ttctgagaaa atgttggagc
    20401 aagctcgttt gaaagcggag cagcaagcaa tcaagaatat ccagtttttg gagcaagatt
    20461 taccgaaaaa tcccttggag aaagagtttg attgccttgc tgttagtcgg gttcttcatc
    20521 atatgcctga tttggatgcg gctctctcac tatttcatca acatttgaag gaagatggga
    20581 aactcatcat tgctgatttt accaagacag aagctaatca tcatggattt gatttagctg
    20641 aactggaaaa caagctaatt gagcatggtt tttcatctgt gaatagtcag attctctata
    20701 gcgctgaaga cctgtttcaa ggaaattact cagaattctt tttaatagta gcccaaaaat
    20761 cactcgccta gtcagggagt gatttttcta taaggatgga aaaaagaagg gaaatttgat
    20821 aagataggaa tatggatttt gaaaaaattg aacaagctta tacctattta ctagagaatg
    20881 tccaagtcat ccaaagtgat ttggcgacca acttttatga cgccttggtg gagcaaaaca
    20941 gcatctatct ggatggtgaa actgagctaa accaggtcaa agacaacaat caggccctta
    21001 agcgtttagc actacgcaaa gaagaatggc tcaagaccta ccagtttctc ttgatgaagg
    21061 ctgggcaaac agaacccttg caggccaatc accagtttac accagatgcc attgctttgc
    21121 ttttggtgtt tattgtggaa gagttgttta aagaggagga aattactatc ctcgaaatgg
    21181 gttctgggat gggaattcta ggcgctactt tcttgacctc gcttgataaa aaggtggatt
    21241 acttgggaat ggaaatggat gatttgctga ttgatctggc agctagcatg gtagatgtaa
    21301 ttggtttgca ggctggcttt gtccaaggag atgccgttcg tccacaaatg ctcaaagaaa
    21361 gcgatgtggt catcagtgac ttgcctgttg gctattatcc tgatgatgcc gttgcgtcgc
    21421 gccatcaagt tgcttctagc caagaacata cttacgccca tcacttgctc atggaacaag
    21481 ggcttaagta cctcaagtca gacggatacg ctatttttct agctccgagt gatttgttga
    21541 ccagtcctca aagtgatttg ttaaaagaat ggctgaaaga agaggcgagt ctggttgcta
    21601 tgattagttt acctgaaaat ctctttgcta atgctaaaca atctaagact atttttatcc
    21661 tacagaagaa aagtgaaata gcagtggagc cttttgttta tccacttgct agcttgcaag
    21721 atgcaagtgt tttaatgaaa tttaaagaaa attttcaaaa atggactcaa ggtactgaaa
    21781 tataaaatag attttgttat aatagttgaa aacgcttaaa aaggggtatc atattatgac
    21841 aaaaacaatt gcaatcaatg caggaagttc aagtttgaaa tggcaattat acttaatgcc
    21901 agaagaaaaa gtattggcga aaggtttgat tgaacgtatc ggtttgaaag attcaatttc
    21961 aactgtaaaa tttgacggcc gttctgaaca acaaattttg gatattgaaa atcatataca
    22021 agccgttaaa attttattgg atgacttgat tcgtttcgat attatcaagg cttatgacga
    22081 gattacaggt gttggacatc gtgttgttgc tggtggagaa tatttcaaag aatcaacagt
    22141 tgttgaggga gatgttttag aaaaagttga agagttgagt ttgttggctc ctctacacaa
    22201 cccggccaat gcagcaggtg ttcgtgcctt caaggaattg ttgccagaca ttaccagtgt
    22261 agttgttttt gatacttcct tccacacaag tatgccagag aaagcttatc gttaccctct
    22321 accaacaaaa tattacacag aaaacaaggt tcgtaaatac ggtgctcatg gtacaagtca
    22381 ccagtttgta gcaggagaag ctgcaaaact cttgggacgt ccattagaag acttgaagtt
    22441 aattacctgt catattggta acggaggctc aattacagct gtgaaagccg gcaaatctgt
    22501 agacacttct atggggttca ctcctcttgg tggtattatg atgggaacgc gtacagggga
    22561 tattgatcca gctatcattc cttatttaat gcaatataca gaggatttta acacaccaga
    22621 agatatcagt cgtgttctta accgtgaatc aggtcttttg ggagtttctg ctaattctag
    22681 cgatatgcgc gatatagaag cagctgtagc agaagggaat cacgaggcta gcttggctta
    22741 tgaaatgtat gttgaccgta tccaaaaata tatcggtcag taccttgcag tgctaaatgg
    22801 agcagatgcc attgttttca cagcaggtgt cggtgaaaat gcagagaatt tccgtcgtga
    22861 tgtaatctca gggatttcgt ggtttggttg tgatgttgat gatgaaaaga atgtctttgg
    22921 cgttacagga gacatctcaa cagaggcagc taaaatccgt gtcttggtta ttccaacaga
    22981 tgaagaatta gtcattgccc gtgacgttga acgcttgaaa aaataagtga aactaaaaaa
    23041 atattcaata caaggagttg ggaaagttat ttttccagct tctttttctg atgaaattgt
    23101 ccaaaacctt gctatgattg gcttttttga aaaatatggt ataatagtag taatttaata
    23161 gatggagttg agttttgaag aaaaactttc gtgtaaaaag agagaaagat tttaaggcga
    23221 ttttcaagga ggggacaagt tttgctaatc gcaaatttgt ggtctaccaa ttagaaaacc
    23281 agaaaaacca ttttcgagta ggtctatcag ttagcaaaaa actggggaat gccgtcacta
    23341 gaaatcaaat taagcgacgg attcggcata ttatccagaa tgcaaaaggg agtctggtag
    23401 aagatgtcga ctttgttgtc attgctcgaa aaggagtcga aaccttggga tacgcagaga
    23461 tggagaaaaa tctactccat gtattaaaat tatcaaagat ttaccgggaa ggaaatggga
    23521 gtgaaaaaga aactaaagtt gactagtttg ctaggactgt ctctgttaat catgacagcc
    23581 tgtgcgacta atggggtaac tagcgatatt acagccgaat cggctgattt ttggagtaaa
    23641 ttggtttact tctttgcgga aatcattcgc tttttatcgt ttgatattag tatcggagtg
    23701 gggattattc tctttacggt cttgattcgt acagtcctct tgccagtctt tcaggtgcaa
    23761 atggtggctt ctaggaaaat gcaggaagct cagccacgca ttaaggcgct tcgagaacaa
    23821 tatccaggtc gagatatgga aagcagaacc aaactagagc aggaaatgcg taaagtattt
    23881 aaagaaatgg gtgtcagaca gtcagactct ctttggccga ttttgattca gatgccggtt
    23941 attttggccc tgttccaagc cctatcaaga gttgactttt taaagacagg tcatttctta
    24001 tggattaacc ttggtagtgt ggatacaacc cttgttcttc cgattttagc agcagtattc
    24061 acctttttaa gtacttggtt gtccaacaaa gctttgtctg agcgaaatgg cactacgact
    24121 gcgatgatgt atgggattcc agtcttgatt tttatctttg cagtttatgc gccaggtgga
    24181 gtcgccctat actggacagt gtctaatgct tatcaagtct tgcaaaccta tttcttgaat
    24241 aatccattca agattatcgc agagcgcgag gccgtagtac aggcacaaaa agatttggaa
    24301 aatagaaaaa gaaaagccaa gaaaaaggct cagaaaacga aataaataag gaggaatctg
    24361 gtaatggtag tatttacagg ttcaactgtt gaagaagcaa tccagaaagg attgaaagaa
    24421 ttagatattc caagaatgaa ggctcatatc aaagtcattt ctagggagaa aaaaggcttt
    24481 cttggtctat ttggtaaaaa accagcccaa gtggatattg aagcgattag tgaaacgact
    24541 gttgtcaaag caaatcaaca ggtagtaaaa ggcgttccga aaaaaatcaa tgatttgaac
    24601 gagcctgtga agacggttag tgaagaaacc gttgaccttg gtcatgtggt taatgctatt
    24661 aaaaaaatag aggaagaagg tcaaggtatt tctgatgaag tcaaggctga aatcttaaaa
    24721 catgaaagac atgccagcac tatcttagaa gaaactggtc acattgagat tttaaatgaa
    24781 cttcaaatcg aggaagcgat gagggaagaa gcaggcgctg atgaccttga aactgagcaa
    24841 gatcaaactg aaaatcaaga cttgaaagag atgggcttga aggtcgagca aagttatgat
    24901 attgcccagg tggctacgga tgtgactgcc tatgttcaag cgattgtgga tgacatggat
    24961 gttgaggcta cactttcaaa tgattataac cgtcgtagca tcaatctaca aattgacacc
    25021 aacgaaccag gtcgtattat cggctaccat ggtaaagtct tgaaggcctt gcaactgttg
    25081 gctcaaaatt atctttacaa ccgctattcc aaaaccttct acgttacaat caatgtcaat
    25141 gattatgtcg aacaccgtgc agaagtcttg cagacctatg cgcaaaaatt ggcgaatcgt
    25201 gttttggaag aaggtcgcag tcataaaaca gatccaatgt caaatagcga acgcaagatt
    25261 atccatcgta ttatttcacg tatggatggc gtgactagtt actctgaagg tgatgagcca
    25321 aatcgctatg ttgttgtaga tacagaataa gtaaaatcag gtttatcctg attttttgct
    25381 agttagagga ggttaaactg atgttgaata agataagaga ctatttagac tttgctggtt
    25441 tgcagtaccg taatcctgat aaagcgggag cagagcgaga gaagatgctg gcattccgcc
    25501 acaaaggaca agaggcccga aaggttttta cagaactggc caaagccttt caagcaagcc
    25561 atccagaatg gcaactccaa cagactagcc agtggatgaa tcaggcccag cgtttgagac
    25621 cacatttttg ggtttatcta cagagagacg gacaagtgac agaacctatg atggccttac
    25681 gtttgtatgg gacatctact gactttggaa tttctttgga agtcagtttc atcgaacgta
    25741 agaaggatga gcaaacactg ggcaagcagg ccaaagtttt agacattcca accgttaaag
    25801 ggatttatta tctaacctac tctaatggtc aaagtcaacg gtgggaggcg aatgaagaaa
    25861 agcgtcgtac tttacgcgag aaggtgagaa gtcaagaagt tcgaaaagtt ttagtgaagg
    25921 tagatgttcc tatgacagaa aattcgtctg aagaagaaat cgtagaaggc ttattgaagt
    25981 cttattctaa aattcttccc tattatctag ctacgagaaa ataagataat ttgtaaaaca
    26041 tcataaatca tacagtccaa gagtgaacag tccgctgtgt aattcttggt ctttttgttt
    26101 gcgctttcgc attatataat aaacttacaa aaacaattca aaaggagaac aattatggaa
    26161 gtcgtttcaa gtgttctaaa ttggttttct agcaatattt tgcagaatcc cgcatttttc
    26221 gtaggtttat tggtgttgat aggatatgca cttttgaaaa aacctgccca tgacgttttt
    26281 tcagggtttg ttaaagcaac agtagggtat atgttgctta acgtgggtgc tggtggtttg
    26341 gttacaacct ttcgtccaat cttagcagct cttaactaca aattccaaat tggtgcagcg
    26401 gttatcgacc cttactttgg acttgctgca gcaaacaaca aaattgtagc agagtttcca
    26461 gattttgttg gaactgcaac tacagctcta ttgattggtt ttggaataaa tatcttgctc
    26521 gtagctcttc gaaagattac gaaggtaaga accctcttta ttactggtca catcatggta
    26581 caacaagctg caacagtatc tcttatggtt ctattcttag taccacaatt gcgcaatgct
    26641 tacggtacag cagcgattgg tatcatctgt ggactttact gggcagttag ttcaaatatg
    26701 actgttgagg caactcaacg cttgactggt ggtggcggat ttgcgattgg tcaccaacag
    26761 caatttgcaa tctggtttgt agataaagta gcaggacgct ttggtaagaa agaagaaagt
    26821 ttagacaatc ttaaattacc taagttcctc tcaatcttcc acgatacagt tgttgcatct
    26881 gctaccttga tgctcgtatt cttcggagcc attcttttaa tcttgggtcc agacattatg
    26941 tctaataaag aagtcatcac ttcaggaact ctattcaatc ctgctaaaca agatttcttt
    27001 atgtacatta tccaaacagc ctttaccttc tcagtttact tgttcgtttt gatgcaaggt
    27061 gtccgaatgt tcgtatctga gttgacaaac gccttccaag gtatttcaaa caaattgttg
    27121 ccaggttcat tcccagcggt tgacgttgca gcttcttatg gatttggttc tccaaatgct
    27181 gtcttgtcag gatttacctt tggtttgatt ggtcaattga ttacaattgt tttgctcatc
    27241 gtctttaaaa atccgattct tattattaca ggatttgtac cagtgttctt tgacaatgca
    27301 gccattgcgg tctacgctga taaacgcggc ggatggaaag cggctgttat cctttccttt
    27361 atatcaggtg tccttcaagt tgctctagga gctctttgtg tggcccttct cgatttggca
    27421 tcttatggtg gctaccatgg aaatatcgac tttgaattcc catggcttgg atttggatat
    27481 atcttcaaat accttggtat tgttggttat gtacttgtgt gtctcttctt gcttgttatt
    27541 cctcaacttc aatttgccaa agcaaaagat aaagagaaat attacaacgg tgaagttcaa
    27601 gaagaagctt agtatctaga aaaggagaaa taaaatggtt aaagtattag cagcgtgcgg
    27661 aaatggaatg ggttcatcaa tggttatcaa gatgaaggtt gaaaatgctc tccgtaagct
    27721 taatcaaaca gattttacag tcaattcatg cagtgtcggt gaagctaaag gtttagcagt
    27781 aggatatgac atcgtaatcg cttctcttca tttgattcaa gaattggaag ggcgaactaa
    27841 tgggaagtta attgggcttg ataacttgat ggatgataaa gaaatcaccg aaaaactcag
    27901 tcaagcacta cagtaaaagg ttggaggggg ctggacagaa actgagagtt atcgtttctg
    27961 tccttctccc tctttaaata aaggaggcag atatgaattt aaaacaagct ttaattgaca
    28021 atgactcgat ccgactaggt ttagaggcta acaattggaa agaagcagtc aaggtagcag
    28081 tagatccctt aattgaaagt ggggcaattt tgccagagta ttacgatgct atcattgaat
    28141 cgactgaaga gtatgggcct tactatatct tgatgccagg tatggctatg ccccacgcta
    28201 gacctgaagc tggtgtgcaa agtgatgcct tttcattgat taccttacaa aatcctgttg
    28261 tattttcaga tgggaaagag gtatctgttt tgttggcact agcagcaaca agttcaaaaa
    28321 ttcacacaag tgtagccatt ccacaaatta ttgccctgtt tgaattagaa gattctattg
    28381 cacgtttaca ggcttgccag actaaagaag atgtcttggc tatgattgaa gaatctaagg
    28441 atagccctta tctcgaagga ttggatttgg aaagttagaa agaggaataa agaaatgaca
    28501 aaaagaatac ctaatttaca agttgcatta gaccattcag acttgcaagg agcgattaaa
    28561 gcagctgttt ctgttggtca ggaagtagat attatcgaag ctggaactgt ttgcttgctt
    28621 caagttggaa gtgaactggc tgaagtcttg cgtagccttt tcccagataa gattattgtg
    28681 gcagacacaa aatgtgctga tgctggtgga acagttgcta aaaataatgc ggttcgtgga
    28741 gcagactgga tgacttgtat ctgttgtgca accatcccta ctatggaagc agctctaaag
    28801 gctatcaaga ctgaacgagg agaacgaggc gaaatccaga tcgagcttta tggcgattgg
    28861 acttttgaac aagctcagct ttggctagat gcaggtatct cacaagctat ttatcaccaa
    28921 tctcgtgatg ctcttcttgc tggtgaaact tggggtgaaa aagaccttaa taaggttaaa
    28981 aaactcattg acatgggctt ccgtgtatct gtaacaggtg gtctagatgt agatactctc
    29041 aaactctttg aaggtgttga tatctttacc tttatcgcag gtcgtggaat tacagaggct
    29101 gcggatccag caggagcagc gcgtgccttc aaggatgaaa tcaaacgaat ttgggggtaa
    29161 atcatggtac gtccaattgg aatttatgaa aaggcaaccc caacacactt tacttggcta
    29221 gaacgtttaa attttgccaa ggagttaggc tttgattttg tcgagatgtc tattgacgaa
    29281 cgtgacgagc gtttagcaag acttgactgg agtaaggaag aacgcttgga agttgtcaaa
    29341 gcaatctatg aaactggtgt tcgtattcct tctatctgtt tttcaggcca tcgtcgctac
    29401 ccattgggtt caaaagatcc agttctagag gaaaaatctc tagaactcat gaaaaaatgt
    29461 atcgaattag ctcaagactt gggagttcgt acgattcaat tagctggtta cgatgtttac
    29521 tatgaggaaa agtcacccca gacacgccaa cgttttatca aaaatttgag aaaagcctgt
    29581 gactgggctg aagaagctca ggtggtactt gctattgaaa ttatggatga tcctttcatc
    29641 agtagcatcg aaaaatattt ggctatagaa aaagagattg actctccctt cctctttgta
    29701 tatccagata ttggtaatgt gtctgcatgg cataatgata tctatagtga gttttatctt
    29761 ggtcatcatg ccatcgcagc tctccatctc aaggatactt atgcagtgac agaaagttca
    29821 aagggccagt tccgagatgt acctttcggg caaggttgtg tcaaatggga agaagctttc
    29881 gatattttaa aggaaaccaa ttataatgga cctttcctaa tcgaaatgtg gtctgaaaat
    29941 tgtgaaacag tagaagaaac acgcgcagcc gttcaagagg cgcaagcttt tctctatcca
    30001 ctcattaaga aagcaggttt gatgtaagat gaatcaagta atcaatgcta tgcgtaaacg
    30061 agtctgtgat gccaatcaat cattgccaaa acatggactt gtcaaattta cctgggggaa
    30121 tgtatctgaa gtcaatcgcg aactcggtgt cattgttatc aaaccatcag gcgtggatta
    30181 tgacgaattg acacctgaaa acatggtagt gactgatcta gatggtaaga tcctagaagg
    30241 ggatttaaga ccatcttccg acctcccaac tcatgtgcaa ttatataagg cttggtcaga
    30301 aattggtagt gtggttcaca cccattcgac agaagctgtt ggttgggctc aggcaggtcg
    30361 tgatattcct ttctacggaa caacccatgc agattatttc tacggttcaa tcccttgcgc
    30421 ccgtagtttg accaaggacg aagtagaagt gacctatgaa aaagatactg gcctggttat
    30481 cgtagaagag tttgaacatc gcggacttaa cccagttgaa gtaccaggaa ttgttgtacg
    30541 caatcacggt ccattcacct ggggcaaaaa tccagagaat gctgtttatc actctgtcgt
    30601 actagaggaa gtatcaaaga tgaatcgctt tacagaacaa atcaatccaa gagttggacc
    30661 tgctccccag tacatactag aaaaacacta ccaacgtaaa catggaccaa atgcttatta
    30721 tggtcaaaag taagaacgat gaaggaggag aaaaagataa atttagctcc tctttttaca
    30781 tttgattttt attgagagta aagttggagt tgaagtaatt ttgaaagatt ttttagaaat
    30841 agcgcttgat atatatggta aaataaaaag aattgctgtg atatcaatag atttggggga
    30901 ttttttaata tggtactgga taaggcaagt tgtgatttgc ttcaatattt gatggatcaa
    30961 gaaacgtcca aaacgattat ggcgatttcg aaagatttga aagagtcaag aaggaaaatt
    31021 tattatcaca ttgacaaaat caatgctgct ctggttgacg aggcgcttca catcattagt
    31081 attccacgaa ttggtattca cttaacggaa gagcagagag atgcttgttg taaactatta
    31141 tcggaagtag attcgtacga ttatatcatg agtgcgcatg aacgtatgat gataatgtta
    31201 ctatggatag gtatttctaa agaacgtatt acgattgaaa aattgataga gttaacagag
    31261 gtatctagga atactgttct caatgatttg aatagtattc gttatcaact aactttggaa
    31321 caatatcagg tgatcttgca agtgagcaag tcacagggat accaccttca tgcccaccct
    31381 cttaataaaa ttcaatatct tcaatcgctt ctatatcata tttttatgga agaaaatgcc
    31441 acttttgtat ctattttaga agataagatg aaagagaggt tagatgatga gtgtttgctt
    31501 tctgttgaaa tgaaccaatt ttttaaggaa caggttcctt tagttgaaca agatttaggg
    31561 aagaaaataa accatcatga aataactttt atgttgcagg ttctacctta tttgctgtta
    31621 agctgtcata atgttgaaca gtatcaagaa agacatcagg atatagagaa agaattttct
    31681 ttgataagaa aaagaataga gtatcaggtg tctaagaaat taggagaacg gttgtttcaa
    31741 aagtttgaaa tttctttgtc aggacttgaa gtttctcttg tagctgttct cctcctctcc
    31801 tatcgtaagg atttggatat tcatgcagaa agtgatgatt ttcggcaatt aaaacttgct
    31861 ttagaagaat ttatctggta ttttgaatca caaatccgaa tggagattga gaacaaggat
    31921 gatttgttac gaaatttgat gatccactgt aaagccttgt tatttagaaa gacttacggt
    31981 attttttcta aaaatcctct aacaaaacaa attcgatcca agtatggaga attattttta
    32041 gtcactagaa aatctgcgga aattttagaa ggagcatggt ttattcggct aacagacgat
    32101 gatattgcct atttgacgat tcatattgga ggatttttaa agtatacacc atcgtctcaa
    32161 aaaaatatga aaaaagttta tctcgtttgt gatgaaggtg ttgcggtttc gagacttttg
    32221 ctgaaacaat gcaaacttta ttttccaaat gagcaaattg acactgtatt tacaacagaa
    32281 caatttaaga gtgtggaaga tattgcacaa gttgatgtag tgattactac taatgatgat
    32341 ttggatagca gatttccgat tttaagggtt aatcctatcc ttgaagcaga agatattttg
    32401 aaaatgctag actatcttaa acacaatata tttcgtaata agagcaaaag tttcagtgaa
    32461 aatctttcta gtcttatttc gtcttatatt gtagacagca agttggctag taagttccaa
    32521 gaagaggttc aaacacttat aaataaagaa atagtagttc aagctttttt ggaagatatt
    32581 tgaaggacag tccaatgatg aacacaaacc tgtgtttttc ttggtctttt ttagtgtttt
    32641 gaagggtggt atactaatct caaagataac aattatatcc aaaggaggca acatatgcca
    32701 aacgtcaaag aaattacaag agagtcatgg attttagcca ctttcccaga gtggggaaca
    32761 tggttgaacg aagaaatcga agaagaagtc gtacctgaag gcaactttgc catgtggtgg
    32821 ctaggcaact gtggtacttg gattaagaca ccagctggtg ctaacgttgt catggacctt
    32881 tggtcaaacc gtggaaaatc aaccaaaaaa gtgaaagata tggttcgtgg gcaccaaatg
    32941 gcaaatatgg caggtgttcg taagttgcaa ccaaacttgc gtgttcagcc aatggttatc
    33001 gatccatttg ctatcaacga actagactat tacttagttt cacacttcca cagtgatcat
    33061 atcgacccat acacagctgc agcaattctc aacaatccta agttagagca tgttaagttt
    33121 atcggtcctt accactgtgg acgaatctgg gaaggatggg gtgttccaaa agaacgtatc
    33181 atcgttgtta aaccaggtga cactatcgaa ttaaaagata tgaagattca tgcagtagaa
    33241 tcatttgacc gtacttgctt ggtaactctc ccagtgaacg gtgctgatga gacaggcggt
    33301 gaacttgctg gcttggctgt tacagatgaa gaaatggctc aaaaggctgt taactatatc
    33361 tttgaaacac caggtggaac catctatcat ggtgcagatt ctcacttctc aaactatttt
    33421 gcaaaacatg gtaaagactt taaaattgat gttgctttga ataactatgg tgaaaatccg
    33481 gtaggtatcc aagacaaaat gacatctatc gaccttcttc gtatggcaga aaatctgcgt
    33541 accaaagtca ttatcccagt tcactatgat atctggtcta acttcatggc ttctactaat
    33601 gagattctag aactttggaa aatgcgaaaa gatcgcttgc aatacgattt ccatccattt
    33661 atctgggaag ttggcggtaa gtacacttat cctcaagatc aacacttagt agaataccat
    33721 catccacgtg gttttgatga ttgttttgaa caagactcta acattcaatt taaagctttg
    33781 ctataatata aaaatattct ttggaggtta tccgtatgtt gcatgatacg gataattttc
    33841 atataatagt aaaatagaat gtgtgattca ataatcacct caaatagaaa ggaaattcta
    33901 tgtcaaatct atctgttaat gcaattcgtt ttctaggtat tgacgccatt aataaagcca
    33961 actcaggtca tccaggtgtg gttatgggag cggctccgat ggcttacagc ctctttacaa
    34021 aacaacttca tatcaatcca gctcaaccaa actggattaa ccgcgaccgc tttattcttt
    34081 cagcaggtca tggttcaatg ctcctttatg ctcttcttca cctttctggt tttgaagatg
    34141 tcagcatgga tgagattaag agtttccgtc aatggggttc aaaaacacca ggtcacccag
    34201 aatttggtca tacggcaggg attgatgcta cgacaggtcc tctagggcaa gggatttcaa
    34261 ctgctactgg ttttgcccaa gcagaacgtt tcttggcagc caaatataac cgtgaaggct
    34321 acaatatctt tgaccactat acttacgtta tctgtggaga cggagacttg atggaaggtg
    34381 tctcaagcga ggcagcttca tacgcaggct tgcaaaaact tgataagttg gttgttcttt
    34441 atgattcaaa tgatatcaac ttggatggtg agacaaagga ttcctttaca gaaagtgttc
    34501 gtgaccgtta caatgcctac ggttggcata ctgccttggt tgaaaatgga acagacttgg
    34561 aagccatcca tgctgctatc gaaacagcaa aagcttcagg caagccatct ttaattgaag
    34621 tgaagacggt tattggatat ggttctccaa acaaacaagg aactaatgct gtacacggcg
    34681 cccctcttgg agcagatgaa actgcatcaa ctcgccaagc cctcggttgg gactacgaac
    34741 catttgaaat tccagaacaa gtatatgctg atttcaaaga acatgttgca gaccgtggcg
    34801 catcagctta tcaagcttgg actaaattag ttgcagatta taaagaagct catccagaac
    34861 tggctgcaga agtagaagcc atcatcgacg gacgtgatcc agtcgaagtg actccagcag
    34921 acttcccagc tttagaaaat ggtttttctc aagcaactcg taattcaagt caagatgcct
    34981 tgaatgttgt ggcagctaaa ttaccaacct tcctaggtgg atcagctgac cttgctcact
    35041 caaacatgac ttatatcaag acggatggac ttcaagacga taccaatcgc ttgaatcgta
    35101 acattcagtt tggtgttcgt gaatttgcaa tgggaacaat cttgaacggg atggcccttc
    35161 atggtggact tcgtgtatac ggtggaactt tcttcgtctt ctctgactat gtgaaggcag
    35221 ctgtccgctt gtcagcctta caaggacttc ctgtgactta tgtctttacc catgattcaa
    35281 tcgcagttgg ggaagatggt ccgactcacg aaccagttga gcatttagca ggtcttcgtg
    35341 ctatgccaaa tctaaatgtt ttccgtccag cagatgcgcg tgaaacgcaa gcagcttggt
    35401 accttgcagt gacaagtgag aaaacaccaa ctgcccttgt cttgacacgt caaaacttga
    35461 ctgttgaaga tggaacagac ttcgacaagg ttgctaaagg tgcttatgtt gtatatgaaa
    35521 atgcagccga ctttgatacc atcttgattg cgacaggttc agaggttaat cttgctgtct
    35581 cagctgctaa agaattggct agtcaaggcg aaaaaatccg cgtagtcagc atgccatcta
    35641 cagatgtctt tgataaacaa gatgcagctt acaaggaaga gattcttcca aatgcagtcc
    35701 gccgtcgtgt ggcagtcgaa atgggtgcaa gtcaaaactg gtacaaatat gttggtctcg
    35761 atggtgccgt tctaggtatt gataccttcg gagcctctgc cccagcacca aaagtattgg
    35821 cagaatatgg ctttactgta gaaaatcttg taaaaattgt tcgaaacttg aaataatcct
    35881 aaaaatcagg gcgtaagctc tggtttttct taccagaaaa gtaaggtaca atcttgtaaa
    35941 agtagctgaa atttgatata gtagtcctat gtaaaagaca aaggagaaaa caatgaatcc
    36001 aaatattact tttttaatca tgcttgtagg tatgatggcc ttgatgttct ttatgcaacg
    36061 ttctcaaaag aaacaagctc aaaagcgtat ggaaagctta aataaactac aaaaaggcta
    36121 tgaagtgatt acaatcggtg gactttatgg aacagtcgat gaagtagata cggagaaggg
    36181 aacaatagtt cttgacgtag atggagttta cttgactttt gaactagctg ctatcaagac
    36241 agtattaccg ctgaaagaaa cagcttcact agaaggcgca attgaaaaat aagacgggat
    36301 cacgaactcc cgtttttcta taaaagaaag gaaatgggat gaaaaaatta gtctttgtct
    36361 gtctgggaaa tatttgccgt agccctatgg ccgagtttgt tatgaaatca atgacagata
    36421 actacgaaat ccaaagtcga gcaacttcct cttgggaaca tggcaatccg attcataagg
    36481 ggactcaggg aatttttcaa gagtatgaga ttccttatga caagaacaag acatcgcttc
    36541 agattagtaa ggaagatttt gaagcctttg attatattat cggaatggac gcttcaaata
    36601 tttctgactt atgtcagatg tgtccagtag actgtcaaga taagatttac tcattttcat
    36661 ctgaaagtgt tccagaccct tggtatacag gagattttga agaaacctat cgacgtgttc
    36721 aagagggctg tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt ggaaaatctt
    36781 acgaattttt acgaaaagta tcgtgtctat ctgactcgtc cacgtttaga gcttttggca
    36841 gtagttacca ttgttttctg tgctgtactc gtcttttttc taaatattcc aggaaaaggt
    36901 gtcttaaaac tcgataatgg aacgattgtt tatgatggca gtcttgtccg cggtaaaatg
    36961 aatggccaag gtaccattac cttccaaaat ggagaccaat atacaggtgg cttcaacaat
    37021 ggagccttca acggaaaagg tacctttcaa tctaaagaag gctggaccta cgaaggtgat
    37081 tttgtaaatg gtcaggctga aggaaaaggg aaactaacaa cagaacaaga agtcgtttat
    37141 gaaggaactt ttaaacaagg cgtttttcaa caaaaataaa gcctccttat caaaggaggt
    37201 attattagaa tcgcaaggta agcgcttacc tgcaaatccc tttctttcca aatccctctt
    37261 ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt caaagataaa
    37321 acctgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc tttgtgaaat
    37381 gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt gaaaaaatta
    37441 acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa aaaactgtga
    37501 caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt caaaaagctc
    37561 tagttgccct tgaagaaatg cgtaaattgg atcaagaaca agttgactac atcgttgcca
    37621 aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc tttgaagaaa
    37681 caggacgtgg tgtatttgaa gacaaagcaa ctaagaactt gtttgcttgt gaacacgtag
    37741 taaacaatat gcgccacact aagacagttg gcgttatcga agaagacgat gtaacaggat
    37801 tgactcttat tgctgaacca gttggtgttg tttgtggtat tactccaaca acaaacccaa
    37861 catcaacagc aatcttcaaa tcattgattt cattgaagac acgtaaccca atcgtctttg
    37921 ccttccatcc atcagcacaa gaatcatctg ctcatgcagc tcgtatcgtc cgcgatgcag
    37981 ctatcgcagc tggtgctcct gaaaactgtg tgcaatggat tactcaacca tctatggaag
    38041 caacaagtgc ccttatgaac cacgaaggtg ttgcgacaat ccttgcaaca ggtggtaatg
    38101 ccatggttaa ggcggcttat tcatgtggta aaccagctct tggggtaggt gccggaaacg
    38161 ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat atcgtcatgt
    38221 ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc attgataaag
    38281 aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt gtaaacaaaa
    38341 aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc aaaaactgtg
    38401 ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt gcagaacaag
    38461 caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa gaagttggcg
    38521 aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg aaatctgaaa
    38581 gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt cttggacact
    38641 cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa gctgttaaag
    38701 ctattcgtgt tatctgtaat tcaccttcta cttttggtgg tatcggggac gtttacaatg
    38761 ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca gttggggata
    38821 acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga aataacatgc
    38881 aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa taccttcaaa
    38941 aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta gagcttggtt
    39001 tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt taccaaatct
    39061 ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact gagattatgc
    39121 gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg gatgctgcca
    39181 aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt gtccaaaaat
    39241 tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag actaaattca
    39301 tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc gttatctctg
    39361 ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca actgtggcaa
    39421 tcgtagatcc tgctttggta ttgacagttc caggatttgt tgctgctgat actggtatgg
    39481 acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac tacactgatg
    39541 gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca gttaagaatg
    39601 cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt atggcctttg
    39661 ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg caattccaca
    39721 caatccacgg tcgtacaaat gctatcttgc ttccatacgt tatccgttac aacggtacac
    39781 gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat gaaaaatacc
    39841 aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg gttgaatctt
    39901 acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat tttagagacc
    39961 aaggaattga cgaaaaagaa tggaaagaac attctcgtga attagccttc ctggcttatg
    40021 aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg caagaaatca
    40081 tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa ttgtttatca
    40141 gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt tctatgataa
    40201 aaggcatcct atcaaggttt ttgaacacct gataggatgc ctttttatga tattgaggcc
    40261 tttttgccct ttttgaaaaa ctagaataga aacaaaatat ataatagatt gaaactggaa
    40321 tagtacactt ctgcttctaa aacattgtta gaattcgatt tgactgtcct gatcgatttg
    40381 tcctattcct atttcatttt attatataaa aaatatagta tagtagactg aatctaaaat
    40441 agtacgaaac aattgctaaa acatttatag aaattaattt tactttcctg atagagttgt
    40501 tcacatctta tttcaattca ctatagttta atttaagagt agtatttact aaggcccaat
    40561 taaaatcaaa gagcaaacta gaaaacgagt gccattcagc tcaaaacact gatttgagat
    40621 tgcagataag actagccccc tcattaacag atttacgata aaacgatgac aaggtgtgtt
    40681 gctttttgat ttctaaagag tataatgata gatctctata aaataagtgc gaaggaaatg
    40741 agcttttata gtcctttcgt tttaaaatac tatctcagat attcttatat cgacaagaag
    40801 tttttgagtc attccctcat catacatatt aaataaatag tggctcattc aatttttcac
    40861 tagaataata agctagtata gtaaactgaa ataagatata aacaaataaa ttggagctta
    40921 acatccattt ccagcaattt tttagaaact acagtggact attctagatt caacatatta
    40981 taaaaactag agtaaaagaa aaggattgga tcttgtgtaa tgcaggatcc aatcctttca
    41041 atcattttgt ccaacttttg gaggttccta caatgtagtc gtcattaata aagacagatg
    41101 ggaatgacag tgttcctatt tattttgata gagatcgatg aattctttag atagcaactg
    41161 aataatctct gttgaagcca tttggtcttc tgcatgcata aatagcaagg agaatcctat
    41221 tttttctcca gtagcttctt tttgtatgag attagagtga atcttgtgcg cttctactaa
    41281 ggagtcttcc gcttcttcaa ctttaatttt cgcttctttt aaatttcctg ccttagctag
    41341 ttggatggct tcaataaagg atgatttggc tgctccacta ttggcaatga gctgaaaaca
    41401 gatatattcc atttcttctg tcatcttatt tctcctatcc atgcaagtgc ttgttccaga
    41461 acttttgctc cattcatcat tccgtaatcc cgcatatcaa tggtatctac agggatattt
    41521 cctgcaattt ctttcacagc aagtaactca taacgaattt gtggcccaat tagaatgaca
    41581 tctgcttcat ggatattctt tttagcttct gtcattgatt ttgcttggat agagatttca
    41641 atcccacgtt cagtcgcact ttgttgcatt tttttaacaa gcatacttgt cgacattccc
    41701 gcattacata ctaataaaat ttgtttcata atcttaacct tccatttctt gttcaacaac
    41761 tttgtcatta actttgataa atggaatgta tagaagaact ccaagtgcaa agatgatgaa
    41821 ttgaactaga actgctctca cgtcccctgc tgttgctaac catgcattta agaatactgg
    41881 tgtagtccaa ggaacttgta taaatgcagg actcatgaat cctgtaactg ttgctaagta
    41941 gctgattaaa ataccaagga ctggaactgt gataaatgga atagctaatg aaatgttata
    42001 aacgattggg taaccgaata atactggttc attgatattg aagataccag gtccaaaaga
    42061 taatttagcc acgtttttag agacagcatt gcgactcact aagaatgttg ctattaataa
    42121 acataatgta gatccactac cacccattaa agcgaatgtt tgtatttgtg ataggttgat
    42181 gatgtgtgga atggcttgtc cattatttgc tgcagtgatg ttttcagtaa tgttaattaa
    42241 tagtaatggt tctaggatgg cactgtaaat aactgcttgg tgaataccaa atagccataa
    42301 catatttcct aaagagtaaa taataatgac cccgattaag cttgtaccaa tatgacgaat
    42361 tggttcttga ataaagattg taatgattga gattaagttc attccagtta tattgaataa
    42421 taatgctgaa acaaccccaa ataaggagat gacggtcatg actggaagta atacgctaaa
    42481 tgatctacta acagctggtg gaatattttc accaaggttc atttgtaaag ctttaacgtt
    42541 tgataattca atgaataatt ctgttgcaat aatcgctacg ataaccccgg cgaacattgc
    42601 gcctgtacct gtgttgttga atgaaagaac acctgaaatg tttaccgcat cttttgctcc
    42661 gtcaggaact acagaaactg tatttggcat catcacaatt aaagaaacta atgatagcat
    42721 tgatgctgct aacgggtttt cgaaatctct gtttttagct aagaaataac caaccattac
    42781 agcaataatc atacctgaaa tacttaaagt accgtttgca attgttattc cccaatattg
    42841 gaatcttgtt aatgtatccc cttggaaaat ccacttaaat accgtgttgt tcaaaagaac
    42901 gattaaacct gccaaaatat ataatggcat tactgttacg aatgcatctc ttagggtttt
    42961 taaatgaatt tggttcccta gtttaccagc aaaggtggca aattgatcta taaattttga
    43021 gtttttcatc tgaatatctc ctctctttac tcctcaattt ctacttcaaa accgttattg
    43081 tcacttactt tgcgatacca tcttcctgag ctcttgatgg ttctcttttg cgtttctaaa
    43141 tcaacggaga taaatccgta acgattctta tatgcattat tccaagacca gcaatcaaat
    43201 gcggtccaag cgtggtatcc aaaacagtga cttccctctt caatggcttt atgtagccat
    43261 cttaaatgtt cttcataaaa ttcaatacga tacacgtcat cgataactcc attttcatcg
    43321 ataaaccgtg cttcgttttc aacacccatt ccgttttcac tgataaacca tggaatatta
    43381 tcatattctt ccttcacaat catagcaata tcataaatgg ctttcggaaa aatttcccaa
    43441 ccacgatatg gattcatgcg acgctccggc cattcatact ctttgaagta ttggtctggc
    43501 atccagggcg tctgatattc ctcaggattt gcttgtgctt gaacacgttt tggatggtag
    43561 tagtttactc caagaaaatc aaccgtattt gatttcatca gttgaagctc tttttcggta
    43621 tgactccata acacgccatc tctctctagc tgttttacca atctttctgg gaaagttcct
    43681 ttaacagctg gattcaagaa gactttgtta aagaagtctt ctgtaaatcg acttgcttct
    43741 aagtcttctg gagaattact tcttggataa gcaggtgtca agtttaaaat aatcccaatc
    43801 tttccatcaa gtcctaatga gcgatatagt tgaatcactt ttgcactagc aaggtttaga
    43861 ttatagatga cttgtacggc ctcttttccc tttcctttta gatttggata atggaaagca
    43921 tataagtatc ctgcttctgg aatgaccatt ggctcattga aagttgtcca gtaatgaacc
    43981 ttatctccaa agcatgtgaa agcagtcttg gcaaacttca cgaataactc cactacatgt
    44041 ttgctttccc aaccaccgta tttttgaaga agttccactg gtaaatcaaa atgatgtaaa
    44101 ttcatcacaa gatccatctg gttcttttta gcttcttcaa tgatggcatt gtagaaagca
    44161 ataccttttg gatcaggctc acctgtctct aaattcttga tgagtcgact ccattggatg
    44221 gaagtgcgga aagaattaac tccaatttcc ttcatcaagt ggaaatcttc ggtgtaagta
    44281 tgaaaaaagt tactggctac aagaggtccg acattatcga aaaaatcttc tggatgcgtt
    44341 ttgaaccagt agtccatcac attttcatgt acttttccat attgaccttc tgtctgaggt
    44401 ccgctagaag cagctcccca ccaaaacaaa ttaggaaata ctaacttttt tttcatgctc
    44461 aacctccttt ctattattct atacataaag tatagcataa ttttaaaatt tgtctagaca
    44521 tttttaaaaa ttgttgatac atatcttgtg tttgcctcat ttcttgaact ttgttatact
    44581 actattagaa tattatagag agaaccagat taaatgagta caaaatattt atttatttac
    44641 aatgagattc gtgaaaagat tctttgtaat aaatatacca tgaacgaaca attgcctgat
    44701 gaaatgacat tagctaaaca gtttgcctgt agtcgaatga cgatcaaaaa agctttagac
    44761 ttgttagttt ctgagggctt aatttttaga aaacgtgggc agggaacctt tgttctctct
    44821 cgtggcagct caaaaagaaa attaatcgtt ccagaaagag atatccgggg actgacaaaa
    44881 atatctgaag atgctcattc tacaattgac tcgaggatta ttcacttcaa attagaattt
    44941 gcaaatgaat ttttagcaga aaaactacag gtcgctttgc agagtccagt ttataatatt
    45001 taccgcctgc gtattattga cggtaaacct tatgttctgg aacaaactta tatgagtacc
    45061 gatgttattc caggtattac tgaagatatt ttacaaaaat cgatttacaa ttacattgaa
    45121 ggaaagttag gattgcatat tgccagtgct acaaaaatct tgcgagcttc ttctagttca
    45181 gaaaatgagc aacattactt gcagctcctt ccaacggaac cggtatttga agtagaacaa
    45241 gtggcttatt tggataacgg aactccgttt gagtactcga ttagtcgtca tcgctatgat
    45301 ttatttgaat ttaattcttt tgcattacga cattcctcct aggagaaaat gtgaaaatga
    45361 agccaatctt ttacagactc tagtttaaga aaaatttaaa acagggcaag aaggtcccat
    45421 ctatgcttaa atggtttctc ttttctaaat aagatagctt taaaagagtg atcgttttat
    45481 ccatcatgtt gaaaaatatc ttcgtatagc ttatagagta ggtactgaaa ttgttcacct
    45541 gatctacttc ttatagttat ttagttttaa atagtgtttc aaacattctt acactgacga
    45601 gaagtttttg agtcttttct tgtaacacat atagtatact gaggttagaa tagtagactg
    45661 tgacttctaa aaaattgcta gaaatgaatt tcaatctccc aatttatttg ttcatatctt
    45721 cttttaatat attaaataaa ttctgaatca taatcattta aaaaaatttt attttttatt
    45781 tttcattacg aataatatag atgaagggga aagagtatga aaacagaact gtttcttttg
    45841 ctattagttc aaaaggagaa aaaatgaaag tagaaaatat ttcgtatagg gtggatcatc
    45901 gtatattgtt tgataatatt tcttttgata cttcgagttc aggcgtgaca ttaattactg
    45961 gtaaaaatgg tacaggaaag tcaactttac tatagtagat tgaaactaga atagtacaca
    46021 tctacttcta aaatattgtt agaaatcgat ttgactatcc tgatctattt gtcctgttct
    46081 tatttcattt cactacatct caaattgagt atgacgaagt gcgctcccat gtcctgggaa
    46141 cgcactttct tcatattttt catattcttg aatccatcga taaagactat tgggatgaat
    46201 ttttaaagtt gaactaatca tttttacagg atgaggttta cagcagagag tttgaaggct
    46261 ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat tttgtgtcta
    46321 ctttattata ccaagtccac ccttaagtta gataataaat ctaacttaag gaagctagaa
    46381 ggatgagaat ccaggtggtc aagagtccca aacttaagct gatggggaca cccagaataa
    46441 tttgcttttt gaaggcaagg ccacgttcct ctatattggg aagtgagagt tgaatgagag
    46501 aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg gtggctgttg
    46561 tgagtaagtg aatatcaatc tggggatttt gagcactgat gatagcaatg atgggaaaga
    46621 gggctggagc tacaacggat agggtggaac taaagagtga catcactccg gctatcacac
    46681 aaaagaacag aggtaaccag aaatgaggaa tggatgttgt catgaggtgc cctatcagtg
    46741 tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag agcatgataa
    46801 ttgtagccca gggaacctta gctaaaatgg cttcttgctt ccctaatttg agccttaagg
    46861 cgaggcagac catgagtatt gagacaaagc caatatcaaa tgttttttga taagtagcta
    46921 tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccaaacc aaaaccatgc
    46981 tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt ggttggtcaa
    47041 tagtcaagga tgagtttgtt tttcccttac tatagtgact gtaacaggat aataaaagca
    47101 agacgatgag tgggtagata atgctgacga taaagatatg attgccaagt gaaaaagctt
    47161 gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag ctactggtta
    47221 tcaaattagc ccctcctgaa gctccccaat tgacggcttg agctccaatc aaagggtgtt
    47281 tgtccgcttt ttgacagagg gtaatcgcta gaggacagca aacggccata gtagtgaaaa
    47341 atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg tagagggcgt
    47401 tagggtaggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga gtaccgttag
    47461 ttattgcaaa gttataaaag agagagacgc taaaaatggt aaaaaagagt gaggttggcc
    47521 aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg aggtaagaaa
    47581 aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca atggctagag
    47641 caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta gaatatggaa
    47701 ataggttggc aattttggcg ctcaaaagac cacctgtttc ctattggtac ttgactatta
    47761 tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac acttgttaga attaaaaatg
    47821 aggtattcag gcttgtgatt tcctatggaa gttaatagtg attgcctcta atgcttacaa
    47881 gtgatattaa aaatagagga cttagtgatg tcaatcattt caactgattt aacccctttt
    47941 caaatagatg atacattgaa agcagccttg cgagaagatg ttcattccga agattacagt
    48001 accaatgcca tttttgatca tcatggccaa gccaaggtgt cgctttttgc caaggaagct
    48061 ggtgttttag cggggctaac cgtttttaaa agggttttta ccctatttga tgccgaggtg
    48121 accttccaga atcctcatca atttaaggat ggggatcgtt tgactagtgg cgatttggtt
    48181 ttagaaatca taggctcggt gagaagtctc ttaacatgtg aacgcgttgc cttgaatttt
    48241 ttacaacatt tatcagggat tgcttcgatg acagctgctt atgtagaagc cttagacgat
    48301 gattgcatta aggtatttga tactcgaaaa actactccta atttacgtct ttttgagaaa
    48361 tatgccgtga gagttggcgg tggctataat catcgcttta atttatcaga tgctatcatg
    48421 ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa aggcaattgc tcaagcgcgt
    48481 gcctatgctc cttttgtgaa aatggtcgag gtggaagtgg aaagccttgc tgctgccgaa
    48541 gaagctgcgg cggcgggtgc tgatattatc atgttggata atatgtcatt ggaacagatt
    48601 gaacaggcca ttaccctaat tgcaggacgt tctcggattg aatgttctgg aaatattgat
    48661 atgaccacta ttagccgttt tcgtggttta gcgattgatt acgtctccag tggtagttta
    48721 acccatagtg ctaagagtct tgatttttcc atgaaggggt taacctacct tgatatctaa
    48781 gttgtaaaat aaactaactt tttaaaggat gtctttcctc tagaacgagt tttatgtcag
    48841 atagtttaaa cgcctcttca aatatagtaa aatgaa
//
LOCUS       DAVDWM010000012        41437 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVDWM010000012 DAVDWM010000000
VERSION     DAVDWM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.012"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            237..575
                     /locus_tag="VUW49_000836"
     CDS             237..575
                     /locus_tag="VUW49_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7980781.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            765..2201
                     /locus_tag="VUW49_000837"
     CDS             765..2201
                     /locus_tag="VUW49_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET7980782.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            2253..2492
                     /locus_tag="VUW49_000838"
     CDS             2253..2492
                     /locus_tag="VUW49_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET7980783.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2622..2936
                     /locus_tag="VUW49_000839"
     CDS             2622..2936
                     /locus_tag="VUW49_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET7980784.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3054..5033
                     /locus_tag="VUW49_000840"
     CDS             3054..5033
                     /locus_tag="VUW49_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET7980785.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLLEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVEGLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            5043..5357
                     /locus_tag="VUW49_000841"
     CDS             5043..5357
                     /locus_tag="VUW49_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET7980786.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5388..5894
                     /locus_tag="VUW49_000842"
     CDS             5388..5894
                     /locus_tag="VUW49_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980787.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKVYQQFVQ
                     LEKNSLSL"
     gene            5974..7320
                     /gene="celB"
                     /locus_tag="VUW49_000843"
     CDS             5974..7320
                     /gene="celB"
                     /locus_tag="VUW49_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET7980788.1"
                     /translation="MYQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7700..7876
                     /locus_tag="VUW49_000844"
     CDS             7700..7876
                     /locus_tag="VUW49_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980789.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8277..10490
                     /locus_tag="VUW49_000845"
     CDS             8277..10490
                     /locus_tag="VUW49_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET7980790.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10712..11188)
                     /locus_tag="VUW49_000846"
     CDS             complement(10712..11188)
                     /locus_tag="VUW49_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET7980791.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11411..14614
                     /locus_tag="VUW49_000847"
     CDS             11411..14614
                     /locus_tag="VUW49_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET7980792.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     TITISSPEKLRAALHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WLHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTVMYNAERGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWSTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESFDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELERSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14967..15352
                     /gene="tnpA"
                     /locus_tag="VUW49_000848"
                     /pseudo
     CDS             14967..15352
                     /gene="tnpA"
                     /locus_tag="VUW49_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15731..16360)
                     /locus_tag="VUW49_000849"
     CDS             complement(15731..16360)
                     /locus_tag="VUW49_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET7980793.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(16370..17371)
                     /locus_tag="VUW49_000850"
     CDS             complement(16370..17371)
                     /locus_tag="VUW49_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET7980794.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESIQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17402..18043)
                     /locus_tag="VUW49_000851"
     CDS             complement(17402..18043)
                     /locus_tag="VUW49_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET7980795.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(18062..18877)
                     /locus_tag="VUW49_000852"
     CDS             complement(18062..18877)
                     /locus_tag="VUW49_000852"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET7980796.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19148..19576
                     /locus_tag="VUW49_000853"
     CDS             19148..19576
                     /locus_tag="VUW49_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7980797.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTEEEEEDFESGI"
     gene            19588..20778
                     /locus_tag="VUW49_000854"
     CDS             19588..20778
                     /locus_tag="VUW49_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HET7980798.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20789..21280
                     /locus_tag="VUW49_000855"
     CDS             20789..21280
                     /locus_tag="VUW49_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET7980799.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNASGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21295..22074
                     /locus_tag="VUW49_000856"
     CDS             21295..22074
                     /locus_tag="VUW49_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET7980800.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCFG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            22061..22879
                     /locus_tag="VUW49_000857"
     CDS             22061..22879
                     /locus_tag="VUW49_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET7980801.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            22879..23172
                     /locus_tag="VUW49_000858"
     CDS             22879..23172
                     /locus_tag="VUW49_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7980802.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23194..25095
                     /locus_tag="VUW49_000859"
     CDS             23194..25095
                     /locus_tag="VUW49_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET7980803.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NEKMIRLQC"
     gene            25155..26156
                     /locus_tag="VUW49_000860"
     CDS             25155..26156
                     /locus_tag="VUW49_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7980804.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26266..26601)
                     /locus_tag="VUW49_000861"
     CDS             complement(26266..26601)
                     /locus_tag="VUW49_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980805.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTTPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26612..26854)
                     /locus_tag="VUW49_000862"
     CDS             complement(26612..26854)
                     /locus_tag="VUW49_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980806.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26865..27407)
                     /locus_tag="VUW49_000863"
     CDS             complement(26865..27407)
                     /locus_tag="VUW49_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET7980807.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(27423..27617)
                     /locus_tag="VUW49_000864"
     CDS             complement(27423..27617)
                     /locus_tag="VUW49_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7980808.1"
                     /translation="MNRVKEFRKELGISQLELAKDISVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            27783..28733
                     /gene="rsmH"
                     /locus_tag="VUW49_000865"
     CDS             27783..28733
                     /gene="rsmH"
                     /locus_tag="VUW49_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7980809.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            28745..29062
                     /gene="ftsL"
                     /locus_tag="VUW49_000866"
     CDS             28745..29062
                     /gene="ftsL"
                     /locus_tag="VUW49_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET7980810.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            29066..31318
                     /gene="pbpX"
                     /locus_tag="VUW49_000867"
     CDS             29066..31318
                     /gene="pbpX"
                     /locus_tag="VUW49_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET7980811.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            31320..32300
                     /locus_tag="VUW49_000868"
     CDS             31320..32300
                     /locus_tag="VUW49_000868"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7980812.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKIDFFFWGVGLLASLLTLAILYLM"
     gene            32909..35014
                     /locus_tag="VUW49_000869"
     CDS             32909..35014
                     /locus_tag="VUW49_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7980813.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFNILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLNPFFRPELLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(35310..35792)
                     /locus_tag="VUW49_000870"
     CDS             complement(35310..35792)
                     /locus_tag="VUW49_000870"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET7980814.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(35887..37371)
                     /locus_tag="VUW49_000871"
     CDS             complement(35887..37371)
                     /locus_tag="VUW49_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET7980815.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVSSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYRETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            37524..39131
                     /locus_tag="VUW49_000872"
     CDS             37524..39131
                     /locus_tag="VUW49_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET7980816.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTVDKVFAYIRKDGGRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            39290..41244
                     /locus_tag="VUW49_000873"
                     /pseudo
     CDS             39290..41244
                     /locus_tag="VUW49_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093127.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
BASE COUNT        12998 a         7009 c         8993 g        12437 t
ORIGIN      
        1 atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg
       61 tcctgctctt gtttcaattg actataaatc gctataagaa aaaatcattt tattggtata
      121 aagaagtgat tgaaagtaat ggcgaaacgt tagataatta gttcataaaa aacaagttta
      181 gatcctcatc taaacttgtt tttttaaaat tgaaggatga ttttggagga tataagatga
      241 cattgactat tcatgtatat tataaaggtg atggagatag tgcaataaag tttgcaagag
      301 aaatgcttga tagtggcctt gttgaagaaa ttagaaatca aaaaggtaac ttgaaatacg
      361 aatatttttt accaattgaa aaagagggaa ctattctcct tattgatcag tggataaatc
      421 aagaagcttt agatgaacat catcaatcaa agacaatgca gaaaatctta gatttaagaa
      481 aaaaatatca cttgcagatg caagttgaac gttatataga agatgattct gggatgccag
      541 aaagcgataa gtcgtttata gacacaggga attagaatta ttgtgagagt ttttgtagtt
      601 tgaaagtatt gaaaatcgta aagatatcat tacgagaata caagaagata ttgttctatt
      661 tccattaaaa attacttctt tccttaacaa taaggaaaaa gaatgcgttt ttatcttttg
      721 gtttaaggtt tataataaga gtatcaaagt caggaggaaa aaagatgact acttttaaag
      781 atggattttt atggggtggt gctgttgctg ctcatcaact tgaaggtgga tggcaagaag
      841 gtggcaaggg aattagtgtt gctgatgtta tgactgctgg tcgtcatgga gtagctcgtg
      901 aaattacttt gggagtttta gagggtaaat attatccaaa tcatgaggcg atagattttt
      961 atcatcgtta taaagaagac atagcacttt ttgctgaaat gggattcaag tgcttccgta
     1021 cctctattgc atggacacgt atctttccaa aaggtgatga gttagagccg aatgaagaag
     1081 gattacagtt ttatgataat ctttttgatg aatgcttaaa gaatggtatt gaacctgtca
     1141 tcactctatc tcattttgaa atgccttatc acttagtgac cgaatatggt ggttggaaaa
     1201 ataggaaatt gattgatttc tttgctcgtt ttgcagaagt cgtatttaaa cgttacaaag
     1261 ataaggttaa atattggatg actttcaatg aaatcaataa tcaagcgaat tatcaggaag
     1321 attttgcacc atttactaac tcaggtattg tatatgagga aggtgataat agagaagcaa
     1381 ttatgtatca agcagcacat tacgaattag ttgcttctgc acgagctgta aaaattggtc
     1441 atgagattaa tccagatttt caaataggtt gtatgattgc gatgtgtcca atttatccag
     1501 ttacttgcaa tcctaaggat atcttaatgg caatgaaagc tatgcagaag cgttattatt
     1561 ttgctgatgt gcatgtttta ggtaaatatc ctgagcatat tttcaagtat tgggaacgaa
     1621 aaggtatttc agttgatttt actgaccagg ataaagaaga tttacttggt gggactgtag
     1681 attacattgg tttcagttac tatatgtcct ttgctataga ctctcatcgt gaaaataatc
     1741 cttattttga ttatcttgaa acagaagatt tagtgaaaaa taattatgtt aaggcttctg
     1801 aatgggagtg gcaaattgat ccagaaggtt tacgttatgc gttaaattgg tttacagacc
     1861 actatcactt accactcttt attgttgaaa atggttttgg agctatagat caagttgcag
     1921 cagatggtat ggtacatgat gattatagaa ttgaatatct aggtgcccat attcgtgaaa
     1981 tgaaaaaggc tgtagttgaa gatggtgttg atttaatggg ttatactcca tggggatgta
     2041 ttgatttggt ttcagctggt accggtgaaa tgcggaaacg ttatggcttt atttatgtag
     2101 ataaagatga caatgggaag ggaagttata atcgttcccc gaaaaaatct tttggctggt
     2161 ataaggaagt tatttcatct aacggtgaat cagtagaata gtataaacaa actgagatta
     2221 ttctcagttt gtttagcata ttgaggtgac atatgattgg aagtattgca gcaattttaa
     2281 caacatttgc atttttacca caagtttttc gtgtagttaa gacaaaggac acaggatcta
     2341 ttgctctagg tatgtatgtt atgcaagtta ttggtattgc attgtggctt gctcatggta
     2401 ttcgtatagg agacttgcct ttgattttag caaatagtgt ttcatttcta ctctcaggaa
     2461 taattttatt ttataaattg aagtataaat aatttttttc catatcattt aggaaaatga
     2521 gggaattgtc attggaaacg ctttatttta taataaaaat agataacaaa taagccaact
     2581 tagcacagct ttgattggat ttaagaaaag gagaaaatat tatggcaaaa gctttaatta
     2641 tttgtgcagc aggaatgtct tcatcactta tggcacagaa aacaacagag tttctaaaag
     2701 gaaaagggaa agatattgta gttgatgcta ttagtgctac tgaaggaggg aaagctatcg
     2761 cagcagcagc ttttgatttg tatcttgtca gccctcaaac aaaaatgtac tttaagcaat
     2821 ttgaagaggc tggttctaaa gcaggaaaac cagttgtaca aatacctcct caagcatata
     2881 ttcctattcc aatgggaatt gaaaaaatgg ctaatttgat tcttgagaat atttgatgaa
     2941 cacatgaaat gtcaataaat atttaggtta agataaattg ttttgagttt gatataaact
     3001 gatttaagtg tataatatgc ttcaatatat gaaaacatac gttttaggag ggaatgaatg
     3061 tgttaaacat taaagagaaa gatatccttc aatttttaat aaaaaataag gaacgttttg
     3121 ttacaagcaa agaactggca gaatatttat cctgttcaga tagaactgtc cgtaatgtac
     3181 tgaaattaat tgagaagaca atgattattc aaggagtccg gttgatttca aaacaaggtc
     3241 aagggtatca aatatttttt gagaatcaag gcgcttacca ggagtttaga caaacttatg
     3301 aactggaaga agactatacg aaaactgcgg tttcaaaagg tgatgatcgt ttagtattta
     3361 tcttaaataa attattattg gagcaagttc cagttctttt tgatgattta gctgatgaac
     3421 tttacgttag tcgttcaaca ttatctcatg atttcagaaa gatacgagtg atgttatcag
     3481 agtataattt gtccattgag agtagggcga ataaaggagt ttatgtctca ggtgaggaac
     3541 gtgataaacg acgttttatc ataaattatt tcctagagaa tcaatttttt aaaacaatac
     3601 attgctatgt aaaatttaac ttttttgctc aaactgttcc attagaagaa tttgctagaa
     3661 ttgttttgga tgaatgccaa gaggccaacc taaaactatc tgattttgta ctacagaatt
     3721 tagtagttca catagcattg tctgtgattc gtttaaaatc cggatttgaa ataaaaaata
     3781 tagactgtca gatgactgat gatgcaatag agagaaaggt tgctcaaaga attctttcta
     3841 aagtaagaga agtaacaaat caggaatttc cagttcagga aatagactac attactttgc
     3901 atttgttagc taagagtcaa caatgtcaaa aaaatcagaa gaatatttct gaagaagttt
     3961 taaaaaaatc tttatttaaa acatttcaga atttaggatt agatgatatg tataactttt
     4021 cttcagattt ccaactaatt gaaggattga taactcatct aatgacacta caagtacgtt
     4081 tagaaagccg gattacctta aataaccctt tggtagatga aattaaacag aattatagtg
     4141 atatattctt tatgactaga gaaattcttg caaatatgga caagttcttg gagtggacaa
     4201 tatcagatga tgaagtagca tatgtaagtc ttcacttttt agctgctatg gaacgtagca
     4261 aagagagtac taagtttaat attcttgcta tttgtgcaac aggatttggt gcagcccaaa
     4321 tgttaagaaa tcgtttggaa acggagtttg ggaagcgagt agaagttgtt gatgtaatag
     4381 gctactatga attgaatcaa gaaaaactta aaggaataga ttttatagtg tcagctgttg
     4441 atttatcaaa tctatatttt caaatcccag tttttaaagt tagtgtgttt cttaaaagtg
     4501 atgagatgga aatgattaga aaagctatgg atcagatgca agtttctagt tatgtacaat
     4561 catcaaagat taacaaattt gaaaacaacg gctttagaca gtattttagt aaagaaaatt
     4621 ttttaatatg tactgaatca gacaaggtaa atttacttga aaagatggtt gagggtttat
     4681 ctgttggtga atcaaatgag tttgaacaat ctttacttta tggaattaaa cagcgcgaag
     4741 aattaagttc agttgttttt tctgaaaaaa ttgcagttcc gcatcctatt cagccctttg
     4801 gtacagaggg aaaggtttcg gttgcaattt gtaaagattc gttactctgg gataatcaaa
     4861 gttcacatgt ccagctagta tttcttctat ctccatcaat atatgggaac gaaggtttag
     4921 caactgtgac taaaaagata gtctctctga cagagaatga tgaattgcaa aatcagctaa
     4981 tatcttgcaa taactttgaa gattttatta acatatttga gaagataaaa taggaggttc
     5041 atatgaatca agaggaactg caggttgcag catttgaaat tatcctgcat tcgggaaatg
     5101 caaggtcaga aatacatgaa gcatttgcaa aaatgagaga aggtagtttt gatgatgcag
     5161 aaagtaaatt aaatcaatca aatgagataa ttcttgaagc gcatcatgca caaaccaaac
     5221 tcttgcaaga atacgcaagt ggagtggaaa ttaaaattga aattatcatg gtacatgctc
     5281 aagatcattt aatgaccact atgacattat tagaagtagc aaaggaaatg ttagctcttt
     5341 ataagaaagt taactaaata tgtgttgtta tataaggagg aaatgatatg gataaaaaag
     5401 aacgacaaaa gattgaacaa caacgtcgag agatggctct aactaatact ttttttaatc
     5461 gatatcttct tctacgttat tcaatagcac tcttcttttt tgggaatatt tattggcttt
     5521 tgaatcagtt tattaatccc tcgcctatta ttattttccc cattatgctc atagtatttt
     5581 ctattttagc aactattgaa caatttaaac tttatggaaa taggaaagaa aagttgggaa
     5641 taacactaat gtttgttaga atacaaatgc ttatttcaat aggattactt gttttaactt
     5701 ggacaagctg gtttaagaat ctttttccga tttttgaaaa taaccaagta gcacgtttat
     5761 ttgtttttgt agttcttcta ttaggtttag ttctgagttt gttagacatt agaagaatta
     5821 aaaaaattta taaacgaaca gataaggttt atcaacaatt tgtccaattg gaaaagaact
     5881 cacttagttt ataatcctga tctagtataa tttgtatttg aaactagtct ttagaaagta
     5941 aaaattatta tgttttatta aggaggtcta tttatgtatc aacaaaacgg gttgtttggt
     6001 tttcttgaaa accatgttat gggaccaatg ggcaaacttg ctcagtttaa agtagtacgt
     6061 gctatcacgg ctgcaggtat ggctgctgta ccatttacta ttgtaggatc aatgtttttg
     6121 gtattcagta ttttgccaca agctttctca ttttggccaa ttgtggcaga tattttctct
     6181 gcttcatttg ataaattcac atcactttac atggttgcaa actatgcgac tatgggttct
     6241 ctatctcttt atttcgttct atcacttgca tatgaattga caaaaattta tgcagaggaa
     6301 gaagaactca atatgaatcc tcttaatggt gccttgcttg ccttgatggc ttttgtcatg
     6361 acagtaccgc aaatcatttt tgatggtgga atgatgaaga ctgtgacaag tctaaaagaa
     6421 ggtgcagtaa ttgcagatgg atgggcaatg ggaaatggag tcgcacgttt tgggacaaca
     6481 gggattttta ccgcaatcat tatggcaatt gtgactgttc ttatttatcg tatgtgtgtt
     6541 aaacataatt gggttattaa aatgcctgaa gctgttccag aaggagtttc tcgtggattt
     6601 accgctttgg ttccgggatt tgttgttgca tttgttgtta tctttatcaa cggtcttctt
     6661 gtagcaatgg gaacagatat ttttaaagtc attgcaattc catttggttt tgtatccaat
     6721 ctgactaatt cgtggattgg tttaatgatt atttatctat tgactcaact actttggatt
     6781 gtaggtatcc acggtgcgaa cattgttttt gcatttgtta gtccaattgc tcttgctaac
     6841 atggctgaaa atgctgctgg cgggcacttc gctgttgcag gtgaattttc taatatgttt
     6901 gtaattgcag gtggttctgg tgcaacttta ggactatgtt tatatattgc ttttgcctct
     6961 aaatctgaac agcttaaagc aataggacga gcatctgtag ttccagcctt atttaatatt
     7021 aatgaaccat taatttttgg attacctatt atctataatc cagccttggc tataccattt
     7081 attttagcac caatggttac tgctactatt tattacgtag cgaattctct aaactttatt
     7141 aagccaatta tcgcacaggt tccatggcca actccagtag ggattggagc tttcttaggg
     7201 acagcagatc ttcgagctgt attagttgct ctagtatgtg catttgcagc attcttagtc
     7261 tatcttccat tcatccgtgt atatgatcaa aaattggtga aagaagagca aggtatctaa
     7321 taagtaaaaa agtatattcg atgatagaat ccatatagtt taaataatga gtgttaatat
     7381 tagcagtgaa gaggtctttt taagatattc ttctctgctt tttcatattt tttcgctatg
     7441 tagggtatga agcaaaatag aaagtagttc gaatagttga taaacaggaa actgctgtta
     7501 tttaaaaaaa acaataaagt aaagtatgtt atcgtagaat acgatacaat agtaaaaaag
     7561 caaaaagaat gcgttttata tttgggtttg aggtttataa aataggataa gcgtgctgaa
     7621 aacttagata tgacaaacaa aataaaattt ttaaaagcta cagagaaaca tggtataatg
     7681 tatctaaaga aggtagcata tgtccttaga tatagataaa gaaaagatga caatcatggg
     7741 aatagctttt gaaaaccgat ccgtttttaa gagtgtttgg tatgctttaa gtacaaatat
     7801 gattgaagga tggcgaccta cggttagtga tgttgaaaaa ttacgagatg aagctcttgc
     7861 tctggggatg acttaatgaa gcgcaactgt tatgaatctt atgactatgt tgatccggat
     7921 aatttatata ccgatccggg ttcttcagtt ctaagaaata agcaggaagg aaaaaagcac
     7981 gtgagcttga atatcgtatg attgctagta agagtttgaa attatttatc aatcctatct
     8041 tagttaatag tttaaaagta gtttgtttca caactgcata agcttagaaa agttgaaaag
     8101 tgataactct ttaataaact cttggtttgt taaaagatat tttgtccact gcattttttc
     8161 aattgactat tatttgtcat agatataaca gatagaacat tgcattcaga ataatgaaaa
     8221 acgcatcaaa aatggtgcgt tctgttttta tgctaccata aaaggaggaa aacaaattga
     8281 aaatttttaa gggagagttt tatcgaatct ctgtattaac agacaagcta gtaaggttag
     8341 aatactctcg aactggaaat tttgaggata gaacgacaca acttatctat aatagagatt
     8401 ttggccaaat ttcgttagat tatatcgaga catcaaatgt actagatatt atgacggact
     8461 attttcatct gcactttaat aaaggagaat ttaacgccga aaatttattt atagaattaa
     8521 aaggaaattt tgccgtatat ggtagtcgct ggtattttgg tgaatctatt gaaacgttaa
     8581 aaggaacagc tcggactctg gataaggcag atggagcaat ctcgttagaa gatggaatta
     8641 ttagccgaaa tggtatagcc ttattggatg attctcaagg atttatttgg gatgaacaat
     8701 ctggttatat tgagagagaa aatcaaattg acctgtattt ctttgcctat gggcatgatt
     8761 atagaggagc aatcagagac ttttaccatt tgactggttc aacacccttg ttgccaagat
     8821 atgctttagg caattggtgg agtagatatt ggccttatac gtcggatgaa tacttggatt
     8881 taatagacag atttgaaaca gagaaaattc cattatctat cggtgtgtta gatatggatt
     8941 ggcatataac tgacattcca gctcgttttg gaagtggctg gacaggatat agttggaata
     9001 gaaacttaat accaaatcca gaacagttat tgcaacaact tcatgataga aagttaaaac
     9061 tctccttaaa tgtccatcct gctgatggga tacgggctta tgaagaagct tatcctcaaa
     9121 tcgcaaaacg attggggtta aatgtagaac tagaagaacc tgctattttt gattttttta
     9181 atccctcttt tagggaagcc tactttaaag atgttcatta tgagctagaa aagcagggag
     9241 tagatttttg gtggattgac tggcaacaag ggacacaagg tatgctagat ccactttggc
     9301 ttttaaacca ttatcactat caggatagtt gtaaaaatgc agaaggtggt ttgattttat
     9361 caagatatgc aggtcctggt agtcaccgct accctgttgg tttttcaggg gatactatta
     9421 ttagttggaa ttccttaaga tttcaaccct attttacagc gacagcatct aatatcggtt
     9481 atagttggtg gagtcatgat atcggtggac atatgttggg ggattatgac gaagagctac
     9541 aaactagatg gctacagttt ggtgttttta gtccgattac tcgattacat agttctagaa
     9601 gtccttttaa tagtaaagaa ccttggtttt tttcagaaac aacatctaag attatgaaga
     9661 aataccttcg tttgagacat cagatgattc cctatctata taccatgaat gtacagacac
     9721 atgaggaagg tgccccatta atcagtccaa tctattattt ctatccagag aatgatgaga
     9781 gctataatgt tccaaaccaa tacttttttg gaacagaact gatggtggct cccattgtag
     9841 aaaagatgga tttgacattc caatctgcaa aagtagatgt atggttccct gaaggtgaat
     9901 ggtatgactt cttttcagag aaaaaataca caggtggtgt gaagttaagt gtttataggg
     9961 acatctcgac tacgcctgtg tttgcaaaaa gtggtgcaat cattcccttg gttggttctg
    10021 agataggtat gggtgttgat ttacctgagg ttgtagattg gtatgtattc ccagggaaac
    10081 aacattcttt tgaaatgctt gaagatcaaa atggtcaaag atataaaaca agattatcaa
    10141 tcgattggga aatgggaatg gtagagttag cattacaagg agattctagt atcgttccaa
    10201 gcaatagaaa acatagaatt cattttaaag gaacgaatgt gtctataatt gaattgccaa
    10261 ataagaatga tacagctaaa tttgaatgga aagataataa aaggacatct ttaaatgacg
    10321 aagtttttag actactaaag acagcttctc ttccatatga attaaaagat agattgttaa
    10381 atcaattcat caatgcgaaa aactctcatg acttaatgaa tatcttgcat catcaggata
    10441 aggaattgag agggcgtttg ttggaaatga tatttactag ccaaaactaa ttgaaataat
    10501 tagaaagtca tttgtttagt gtatatgaag attctgtatg gtattcgtta tcactggtca
    10561 cattgaaatt gtgaaataag cacctacaac atagacttta caacacttca tgtttcatat
    10621 catggggtgt ttttctattt aagtcaaagc caatcaaaaa atctcactat tcatcaggtt
    10681 aaaggaaacc taatcatagt gagattgtga attatagtag agtttgtatc gcctcttcaa
    10741 tttgttttgg gtctgttttt gaagaaaagc gttcaaagac ttgcccatct cgaccgatga
    10801 gaaacttagc gaaattccat tcgactcgtt ttcctagtgg gccggatttc tggtctttta
    10861 accagacata gagagggtct gcttccttac cgttgacctt aatcttggca aaacgtggga
    10921 aggtggtttg aaaatgtagg ctacagaagg cgttgatttc ctctgcgctg ccgggtgctt
    10981 gtcccataaa ctgattgcaa gggaaatcca atatttcgaa gccctgatct tgatagcgtt
    11041 catagagttc ttgaagtccc tggtactggg gcgttaaacc acatccagta gcagtgttga
    11101 caatcaagag aactttgcca cgatagctat ccaagggagt tgcttggttg ttttggttca
    11161 aaacggaaaa atcataaagt gaagtcatga gggcctcttt tctttttcta cattgtaaca
    11221 gtttttactt ttttcgtcga gtcggatgga agatattgtt gaagaataag ccttcaatat
    11281 gatagaaacc gtttacaaat aactttaaaa atggtatgat ttaattaaac cggtttactt
    11341 agttgctata aatctctaat ttgagattta gttcctattg attttacaat atgtttattg
    11401 gagtgtatac atgcaaacaa aaacaaagaa gctaattgtg agtttgtctt cacttgtttt
    11461 atcaggattt ttattaaacc attatatgac agttggagcg gaagaaacga ctacgaatac
    11521 cattcagcaa agccagaagg aagttcagta tcagcaaagg gatacaaaaa atttagttga
    11581 aaatggtgat tttggtcaga cggaggacgg aagcagtccg tggacaggaa gcaaagctca
    11641 ggggtggtca gcttgggtag accagaagaa tagttctgca gatgcctcaa ctcgagtcat
    11701 tgaggctaag gatgggacta tcactatctc aagccctgag aaattaaggg cagcgcttca
    11761 ccgtatggtt cctattgaag ctaagaaaaa gtataaactg cgtttcaaga ttaaaacaga
    11821 tcataaagtc gggattgcca aggttcgtat cattgaggaa agtggtaagg acaagcgatt
    11881 gtggaattct gcaacgacgt caggaacaaa ggactggcag accattgaag cagactatag
    11941 cccgacttta gatgttgata aaatcaagct ggagttattc tatgaaacag gaactgggac
    12001 tgtttccttt aaggatattg agctggtaga ggtagcagac cagctttctg aggattctca
    12061 aacagataaa cagcttgagg aaaagattga tttaccaatt ggaaaaaaac atgttttttc
    12121 tcttgcggac tatacttata aggtagaaaa tcctgacgtt gcttcagtca aaaatggaat
    12181 tttagaacct cttaaggaag ggacaaccaa tgtcattgtc agtaaagatg gcaaggaagt
    12241 gaaaaagatt cctttgaaga ttctagcctc tgttaaggat gcatacacag cccgtttgga
    12301 tgactggaat ggcatcatcg ctgggaatca atactatgat tctaaaaatg aacagatggc
    12361 caaattaaac caggaattgg aaggaaaggt agctgatagc ctatctagta tttcaagtca
    12421 ggcggaccgc acctatttgt gggaaaaatt ttcaaattat aaaatgtctg caaatctgac
    12481 tgccacttat cggaaattgg aggagatggc caagcaagtg accaatcctt cttctcgtta
    12541 ttatcaagat gaaactgtcg ttcgaacagt cagggattcc atggaatggc tgcataaaca
    12601 tgtctacaat agtgaaaaga gcattgttgg gaactggtgg gattatgaga tcggtacacc
    12661 tcgtgccatc aacaatacct tgtctctgat gaaagaatac ttctctgatg aggaaattaa
    12721 aaaatataca gatgtaattg aaaaatttgt accagacccc gaacatttcc gaaagacgac
    12781 tgataaccca tttaaggctc taggtggaaa cttagttgat atgggaagag taaaagtaat
    12841 agctggttta ctgcgtaaag atgatcaaga aatttcttct accattcgct cgattgagca
    12901 agtgttcaag ttggtagacc aaggtgaagg tttttatcaa gatggatcct atatcgacca
    12961 caccaatgtt gcctatacgg gtgcttatgg gaatgttttg attgatggcc tgtctcaact
    13021 gttgccagtc attcaaaaga ccaagaatcc aatcgataaa gataaaatgc aaaccatgta
    13081 ccactggatt gataaatcgt ttgctccttt gctggtgaat ggagagctga tggatatgag
    13141 tcgtggacgc tcgatcagtc gtgcaaatag cgaggggcac gtggccgcag tagaagtact
    13201 aagagggatt caccgaatag cggatatgtc tgaaggagaa accaaacaac gtttgcagag
    13261 tcttgtgaag accattgttc aatcggatag ttattatgat gtctttaaga atttgaagac
    13321 ttataaggat atcagtttga tgcaatcctt gttaagtgat gcaggagtcg caagtgttcc
    13381 aagaacaagt tacctatctg cctttaacaa gatggataaa acagtcatgt acaatgcaga
    13441 gagagggttt ggatttggct tgtcactctt ttccagtcgt accttgaatt acgaacacat
    13501 gaacaaggaa aataaacgtg gttggtatac gagtgatggg atgttctatc tttacaatgg
    13561 cgatttgagt cactatagcg atggctactg gtcaacagtt aatccatata agatgcctgg
    13621 tacaacagag acggatgcta agagagcgga tagcgataca ggtaaagttt taccgtctgc
    13681 tttcgttgga acgagcaaac tagatgatgc caatgcgaca gcaaccatgg atttcaccaa
    13741 ctggaatcaa acattgactg ctcataagag ctggtttatg ctaaaggata agattgcctt
    13801 tttaggaagc aatatccaaa acacttcaac agatactgct gcaactacaa ttgaccagag
    13861 aaaactggaa tcaagtaatc catataaagt ctatgtcaat gataaagaag cctcccttac
    13921 agaacaagaa aaggattatc ctgaaaccca aagtgtcttt ttagaatcgt tcgattcgaa
    13981 aaagaatatt ggttactttt tctttaagaa gagttcaatc agtatgagta aggctttgca
    14041 aaagggagcc tggaaggata tcaatgaagg acagtcagac aaggaagttg aaaatgaatt
    14101 tcttacgatt agtcaggctc ataagcaaaa tggagattct tatggctata tgctcattcc
    14161 taacgtggat cgtgccacct tcaatcaaat gataaaagag ttagaaagaa gcctcatcga
    14221 aaataacgaa acccttcagt ctgtttatga tgctaaacaa ggagtttggg gcattgtgaa
    14281 atatgatgat tctgtctcta ctatttccaa ccaattccaa gttttgaaac gtggagtcta
    14341 taccattcga aaagaagggg atgaatataa gattgcctac tataatcctg aaacccagga
    14401 atcagctcca gatcaggaag tctttaaaaa gctagagcaa gcagctcagc cacaagtaca
    14461 gaattcaaaa gaaaaggaaa aatctgaaga ggaaaagaac cattcggatc aaaagaatct
    14521 ccctcagaca ggagaaggtc agtcaatctt ggcaagtcta gggttcttgc tacttggggc
    14581 attttatcta ttccgtagag gaaagaacaa ctaatttgtt catcatctag atgaattaca
    14641 tgaaactgtg gaaaatagaa aaaatccgaa gaaggccaaa tatcttcgga tttttctttt
    14701 aaaaaaggat aagttgatac ttatcctttt ttacttcatt attatgtgac tatagcgttt
    14761 tgaaactaga atagaacgtt ataactgcta aaagatttct ataaattcat ttgattttcc
    14821 taatcaattt gttcgtatcc tatttcactc cactatataa gtccgtctcg ctccgtgagg
    14881 tgcgcgtcga aggttatacc aaaaaaactc caaacgcgat acaataaagg tgttcaatcc
    14941 aattgtaaag cgaaaggaga aaaaatatgg cacaaaagac tcatagttta tcatacacaa
    15001 agtggatgtg ttctatcata ttgtgttcac ccctaagtat agacgaaaag tcatctataa
    15061 tcaatataga agtagtttag gcgaaatatt tcatcgcttg tgtagttata aaggtgttga
    15121 aatgatcgag ggtcacttaa tgccagacca tgtacacatg ttagtcagta ttccactaag
    15181 gctaagtgtt tcaagtttta tggggtattt aaaaggcaaa agtgcactca tgatgtttga
    15241 caaacacgcc aatctcaagt acaagtttgg gaatcggcat ttctgggcgg aaggttatta
    15301 tgtaagtaca gtagggctta ataaagccac acttaagaaa tatagtcaag attaaagaaa
    15361 ttatccagtg gatgatttct tcacgagtat gaaaatttga gaactagtaa agtatgatat
    15421 atagtaaaat gaaataagaa caggacaaaa tcaatcagga cagtcaaatc gatttttaac
    15481 aatgttttag aagtagaggt gtactattcc agtttcaatc tactatagca ctagataaat
    15541 tgagtgtaaa agaatatgag gatcccttta gggatagtgg taagtaatac caaagtctct
    15601 taaagaggca agtgacgagt caagagcaat aaggcttgaa caacgtgaaa gccagcgtct
    15661 ttaggcgctg gctgatgatt tgggcttata gctctgagat aaaccacccg ttagacgggt
    15721 agttatgatt ttatctgagt gtaatatact gttgggcaat ctcgctgatg cggtcaaagt
    15781 tgccttggga agcgagttta ttgagttcgc caccaattcc aacggcatct gcaccagcag
    15841 cgaaccattg agggatgttg tttaggccga ctcctccggt taccattacg gaaacttgtg
    15901 ggatcggtgc cttgactgca gagatatatg ctggactgag agtactacct gggaagagtt
    15961 tgatgatttc actaccggct tcaagtgcag tcgtgatctc tgtgagggta atacagcctg
    16021 gaatgtacgg tgtgctgtag agattgcaca ttttcgcagt ttcagcatgg aaagatggag
    16081 aaacaacgta atttgctcca gctagaatgg catctctagc agttacggca tcaagcacag
    16141 tacctgcacc gatacaaaca ctctgatcgt cctgatacaa gtctacaagt tccttgatga
    16201 tttgtcctgc atactgattg gtataggcga tttcaatagc tttgataccg cccttgatac
    16261 aagcaatcga ggcttgtagt ccttcttcct ttgtatttcc tcgaatgaca gcgacaattt
    16321 tctgtttttt tagttcaata atcgtatctg atttggtcat gtaattctcc taacgaatga
    16381 tatcttgtgc atttgccagt aaattttcaa tactagttgc ggaagtggag agatggtctc
    16441 ctggaagagt gcatttgaga gttgcgctcg caactgcaaa gtcaatggta gtttttaggg
    16501 aggaatgatg gagtagttgg taaagggcac cagatataaa ggcatccccg ctaccaattc
    16561 gttgatagac tgcagttttt agttggatag actcttcaaa tcgttcttcc agagcatagg
    16621 cttgatagac atttttgtct tgctcatcac tagagcggag ggtatggaaa atggccttga
    16681 aaccataggc ttcttttaaa agtcgcatgc gattttccac ctcttctagg ctagcactgt
    16741 ctcttggaaa catctctaga ttttggtcat caatcatgag aggatcaata ccgaagcaat
    16801 agtcagtaaa acgtgcaaac ttagaaaatt catacttggc ttctaggact gaaatcatct
    16861 ttgttctcag attgagatcc attgaaacga caattcctcg gcgcttggct tcttccaaga
    16921 gtaggagaag gatcgcacgg acctcttgac cgatagctat ggtgattcca ctaaaatgaa
    16981 aatggctaat cccctgaaag agagaatcca tatctagcat gtttggccga atctggctga
    17041 tactcgtatg cttacgatcg tagaaaactt cactttgacg acaaccaaag ccgttctcca
    17101 aatagtagag gccgattcga tcgccaagcc gacagattga actggtatcg atttggtgct
    17161 gtttcaagaa tgtgagaaaa cgatctccaa tctcgttggc agggagtgcg gtaaagactt
    17221 tcgttgagat acccagggct tgcaaattac aagcgatgtt aatttctgat ccgccaaaat
    17281 aagtcgaact ggcaacatga tcgccgatac tggtggcatc taatggtgag attcgaatta
    17341 atggttcgcc aaacagtaat attttcgtca tccattagtt cctttctggt ttagaatagc
    17401 tttacttggc taatacttct ttcaaataag cagcgacttc atcatcttgg cagttttcaa
    17461 agaagtattc ttggaagtat ttgccagaga tggtgtcttt gaggaagtct tggtcgattg
    17521 ttttaaggat ggtcatcaaa tcattgtggg tgatttgttt cacctcgttt aagatacgag
    17581 cgttgcgttg ttcagggatt acacgttctc ttgggtagcc accgcccatt tcttcagcaa
    17641 ataagcgttc aaacatcaat ttgagggtca gttctgcccc ccagccaaat cctttggcat
    17701 aagggataga caaggcgtta ccaccattga tttgagaata aaggtaagcg tcagttgggt
    17761 ccactgctag accacagaca acaccaggga agctgtttaa agcaagcata gcccctacac
    17821 ccgtaccaca gccggtaaca acaaagtcaa ctgcctttgt atttaaaagg atggcagcca
    17881 ttagtccgtt ctgcacataa gttaattgac tttctccttc ttctccacgc ataccatagt
    17941 taaataattg gtagtctttt ttatctgtcg cttcttttag actatcgtaa ataatgtgat
    18001 tcttgctagc ttgactattt tcattgatta atgcgatttt cataagatct tctttctatt
    18061 tttactcagg ttgttttccg atgtaggcta agataccgcc atctacatat aggatgtggc
    18121 cattgacaaa attgctggca tcactagcga gaaagacagc agggcccatc aaatcttcag
    18181 gatttcccca acgtgcagca ggtgtttttg caatgatgaa ctggtcaaat gggtggcgag
    18241 aaccatcttc ttgcaactca cgaagaggtg ctgtttgagg agtggcaata taacctggtc
    18301 caattccgtt acattggata ttggctccac cgtattcaga cgcaatgttg cgggtcaaca
    18361 ttttcaagcc ccctttagca gcagcataag cgctaactgt ttcacgtccc agttcgctca
    18421 tcatcgaaca aatattgata atctttccat gccctttctt tatcatagaa ggaataactg
    18481 cctttgaaac gataaatggt gcgtttaagt caatatcgat gaccttacgg aaatcagcgg
    18541 cgctcatttc gcacattgga actcggcgga taataccagc gttattaacg aggatgtcaa
    18601 tgacaccaac ctcttgttcg atttgcttga ccatggcttg gataccgtcc tcgtctgtca
    18661 cgtcacagac atatccatgg gcttggatgc caacttcacg ataagcttca atccctttat
    18721 tgaccagatc ttgattgata tcgttaaaga caatagtagc gccggcttca gcgtaggatt
    18781 tggcaatagc aaatccaatt ccataagaag cgccggtgat gagagcaatt tttcctttta
    18841 aagaaaactg ctcaattgag aatgatgtat ttgtcatttg atacttcctt tcaaatttgt
    18901 taagacttct ctataaccaa attatattct agtaaaccgg ttttgtcaat aaataaatat
    18961 ttttatttat aaaaagattt attttcctta aaaatggaat attttgaaac ttctgtctat
    19021 taaaatgatt aaaaagataa tgaaagcgct aatatttttt taaaaaaact attgacaaga
    19081 taaaaataaa ggattacaat ggggaatata aagtaaaccg gtaaacctaa acaagaaagg
    19141 agaaaagatg aaaattgtac ttgtagggca tggacatttt gctacaggga tttatagttc
    19201 tttacaattg attgcaggca atcaagaaaa tgtggaggcg attgactttg tggaaggaat
    19261 gtcagcagat gaactcaagc aaaaaatctt acttgcaatt tcaaatgaag aagaagtttt
    19321 aatcctaagt gatctcttgg gaggatcgcc attcaaggtt tcttctacca taatgggaga
    19381 aaatccagcc aaaacaatga atgttctctc gggtttgaac ttagccatgt taatggaagc
    19441 agtctttgct agaatggctc atagctttga tgaggttgtt aataaatcag tagtggcggc
    19501 ccagggcgga gtcgtaaatg gtaaagaatt gttttcaacg gaggaagagg aagaagattt
    19561 cgaatcgggt atttaaaggg taaaagaatg ataaaaaagg ttacgattga aaaaataaaa
    19621 tcgcctgagc gcttcttaga agtaccactt ctgacgaaag aagaagtcgg ccaggcaatc
    19681 gataaggtta ttcggcagtt agaactcaac cttgactatt tcaaggaaga tttccctacg
    19741 ccagctacct ttgataatgt ctatccaatc atggataaca cggaatggac caatggtttc
    19801 tggacaggag aactgtggtt ggcttatgaa tacagtcaac aggatgcatt taaaaacatc
    19861 gctcataaaa atgttctttc tttcctggat cgtgtcaata agagagtaga attggatcac
    19921 catgatctcg gcttcttgta cacaccgtct tgtatggctg aatataagat aaatggagat
    19981 ggagaggcta gagaagcaac cttgaaagct gcagataagt tgattgaacg ctatcaagaa
    20041 aaaggtggtt ttattcaagc ttggggagac ttgggcaaga aagagcatta ccgtttgatt
    20101 atcgactgct tgctcaatat ccaactctta ttctttgctt atcaagaaac aggcgatcaa
    20161 aaatactacg atattgcaga aagtcatttc tatgcttcag ctaataatgt aatccgtgat
    20221 gacgcttcgt ccttccacac cttctatttt gatcctgaga caggtcaacc ctttaaaggt
    20281 gtaacgagac aagggtatag tgatgattca tgctgggcac gtggtcaatc atggggagtc
    20341 tatggtattc ctttgactta tcgtcactta aaagacgagt cctgctttga cttgtttaag
    20401 ggtgtgacca attatttctt gaatcgtctg ccaaaagatc atgtgtccta ttgggatttg
    20461 atttttaatg atggtagtga tcaatcacga gattcttcag caacagctat cgccgtctgt
    20521 gggattcatg aaatgctaaa acatcttcca gaggtggatg ctgacaaaga tatttataaa
    20581 catgctatgc atgccatgct tcgttccttg atcgaacatt atgcaaatga tcaatttacc
    20641 cctggtggga caagtctcct ccacggtgtg tactcatggc attcaggtaa aggagtggat
    20701 gaaggcaata tctggggtga ctactattac ctagaagccc ttatccgttt ctacaaagac
    20761 tggaacctat attggtagga ggagaaatat gacaatgcca aatattatta tgacccgtat
    20821 cgatgaacgg ttgattcatg gacaaggaca actttgggta aaatacctag gttgtaatac
    20881 ggtcattgtt gccaatgacg aagtaagcac ggacaagatg caacaaactc tgatgaaaac
    20941 agttgtgcca gactcagttg ccatgcgttt cttccctttg caaaaggtga ttgatatcat
    21001 tcacaaggct aatcctgctc aaacgatctt tatcgttgta aaggatgtaa aggacgcttt
    21061 aaccttggta gaaggtggtg tccctatcaa agaaatcaat attgggaaca ttcacaatgc
    21121 ctctggtaaa gagcaagtga cacgctccat cttcctgggt gaagaggaca aggcagccct
    21181 caaggaatta agccaaactc atcaagtaac atttaatacg aaaacaactc caacaggaaa
    21241 tgatggagct gttcaagtca acattatgga ctatatttaa aagaggagat cgttatgtcg
    21301 attaatgtat ttcaagcgat tttaattgga ttatggacag ctttctgttt tagtggaatg
    21361 ctgttaggaa tttacaccaa tagatgtatt gttctgtcat ttggtgtcgg aattattcta
    21421 ggtgatctgc ctactgctct tgcaatggga gctattggtg aattggctta tatgggattc
    21481 ggtgttggtg ctggaggtac tgttccacca aacccaatcg gacctggtat ctttggtacc
    21541 ttgatggcta tcactagtgc tggtaaagtc agtccagaag cggctcttgc cctctctact
    21601 ccgattgctg tggcgattca attcttacaa actttcgcct acactgtacg tgctggtgcg
    21661 cctgaaacgg cgatgaagca cttgaaaaac cataatttga agaaattcaa gttcactcta
    21721 aatgcaacaa tttggttgtt tgcctttatt ggatttacct tgggttgctt tggtgccctt
    21781 tcaatggata ccttgttgaa actcgtagac tacattccac cggtattact tacaggtttg
    21841 acagttgctg gtaaaatgct cccagctatc ggttttgcga tgatcttgtc agtgatggct
    21901 aagaaagagt tgattccctt tgtcttgttg ggatatgttt gtgcagctta tctaaacatc
    21961 ccaacaattg gtattgcaat tgtaggtact atctttgctt tgattgaatt ttataacaag
    22021 ccaaaaacag cggatcatgt ggtagaggag gaagcacacg atgactggat ctaacaaatt
    22081 aacaaaacgt gattatctta aaacgtcttt gcgggcattc ttttgtcaaa atggatttaa
    22141 ctatagtaac tatcaagggt tgggatatgc caatgtgatg tatcctgctt tgaaaaaaca
    22201 ctatggagag gatcaggaag gtttctacca agccttggaa gaaaactgtg aattctataa
    22261 taccaaccca cacttcctgc cttttattac cagcttgcat cttgtaatgt tggaaaatgg
    22321 tcgaccaaca aaagaaacac gtagcatcaa gatggccttg atgggaccat tggcaggtat
    22381 tggggattct ctttctcaat tctgtttagc tccattgttc tcaaccatcg cagcttcgtt
    22441 tgctcaagaa ggcttggttg tcggtccaat cttgttcttc cttgcgatga atacgatttt
    22501 aacagcgatt aaattgtcaa ctggtctgta tggatacaaa ctaggaacaa ctgtgattga
    22561 taaactaagc gagcagatgg caacgatttc tcgtattgcc aatattatcg gtgtaaccgt
    22621 aattgctggt ttggcagcga catctgttaa gattatggtg ccgattacct ttgctgcagg
    22681 ggaagttaaa gcagacgcta aacaaagtat cgtaagtatt cagggaatgc ttgataaggt
    22741 tgctccagct cttctaccag ccctatttac acttttagtt tactacttga tcaaagaaaa
    22801 gaaatggaca acatataaac tcgttatttt aacagttatc atcggaatta tcggaagctg
    22861 gcttaagatt atagcttaat ggatacaaca ttgttttatg gaatagtgat tgtcttggca
    22921 gtgagtcccc ttttactgtc aagctttcat tctattcgtc agcaaaagtt gcttcgcaaa
    22981 cagatggagc aacgacaaga gtatttagct tctttaacat ctggtgatga agtgttgtta
    23041 ttgtctggaa ttcatggaaa aatcatttct atcaaagatg acttggtctc cttgcagatt
    23101 gcaaaaggtg tggtcatcta tgtagaaaag gaaagtgtaa tgggaaagac aaaagaactg
    23161 ctttttaagt agttcttttt ctggtaatag ataatgaaag aagaaagaag acaatttttt
    23221 gaaagagtcg atggaaacca atgtcgtgat tatatcttgt cccactgttc aaaagactat
    23281 gagaaagtca agagttccct tgaacgcttg atggacaatc gttttatgtt tgatagtcct
    23341 tgggatatgg agccttgttc aaaaatccat caaatccagc cgatggtatg ggatcaagta
    23401 tttgaagatg atccagaatg gtcttatatg ctcaatcgac aagaatatct cttgcagttt
    23461 atgatagggt atctggtaga aggagataag gactatattc aaaagtgcaa gttctttcta
    23521 tttgattgga ttgagcaggt gagagaattt tctcctcaat ccttgatgac tagaaccttg
    23581 gatacgggta ttcgttcctt tacttggttg aaactactct tgctcctctt gaaatttgac
    23641 ttgctagagg agaaagaact agagaaaatt ttggtcagtc tagagaagca gattgacttt
    23701 atgaaaagcc actatcgcgc caagtacacc cttagtaact gggggatttt acaaacaatt
    23761 ccgatgcttg ctatctatca tttcttttca gataagatgg acctagaaga agcttaccat
    23821 tttgcttcag aggagttgaa acagcaaatt gagacacaga ttttaggaga tggaagccag
    23881 tttgaacagt cgattctcta tcatgtagag gtttataaag ccttgctgga tttgtgtctc
    23941 ttgcttccag acttgcaaga tagttaccaa gagttgctgg aaaagatggc gacctatatt
    24001 caaatgatga caggcttaga tggacggact ttggcttttg gtgatagcga ttctacagaa
    24061 acgacagaaa ttttgagcct gtctgctgtg gttttgaaca aggaagacct tcttaacggt
    24121 ctggatgtta aagtcgattt gcttagcctc ttgttcctgg ggcgagaaaa ggtcaagcga
    24181 ctgcaggaat ttgaaaagag agcttggcag cctaagtcca tgatctttga agactctgga
    24241 catgtctgca ttaaggatga acatcgttat ctatttttca aaaatggtcc gctaggaagt
    24301 gcccatagcc atagtgacga gaatagtttt tgcttacagt atcaaggcca acctattttc
    24361 atagatgctg ggcgttattc ttatcgggag atatatgaac gttatctctt aaagagtgct
    24421 tggagtcatt cgacctgcat tgtagatggg aaagctccgg aaagaatcac gggatcctgg
    24481 gaatatgaat actatcctca ctccctgttt tgtcaccata aagaaaggga gggagtgcat
    24541 tatattgagg gagcttattg gtcagcagaa cctgatttgc cttatcttca caagagaaaa
    24601 atcctcatgt tggtagagga tgtctggctc ttggtagatg acatcaggtg tcaaggtcag
    24661 catgaggcgt tgactcagtt tatccttgac aaggatgtga cctatcaaga tgggaaaatc
    24721 aatcagttga gactatggag tgaagttgat tttgatttgg aagataccat catttctcct
    24781 aaatacaatg agcttgaaag aagtagcaaa ctcaccaagc gccaattctt tgagaatcag
    24841 atgctggatt ataccatcat tgcgcatgag agttttgaaa tcatccgtca ttctgtctac
    24901 cagacagatg atcgtgaagt ggaaaatgct ctggcttttg aagtgaaaaa tgacgaaaca
    24961 gacaagctga ttctgttatt aagcgaggat attggtgtag gtgaaaaatt gtgcctcgtt
    25021 gacggaacaa aaatgcgtgg aaaatgtcta gtatatgata aaataaatga gaaaatgatt
    25081 cgcttgcagt gctagaaata ggcattttga atagtgaata tgttataata agtattagta
    25141 ggaggtgttt tagattggag aagaaactga ccataaaaga cattgcggaa atggctcaga
    25201 cctcgaaaac aaccgtgtca ttttacctaa acgggaaata tgaaaaaatg tcccaagaga
    25261 cacgtgaaaa gattgaaaaa gttattcatg aaacaaatta caaaccgagc attgttgcgc
    25321 gtagcttaaa ctccaaacga acaaaattaa tcggtgtttt gatcggtgat attaccaaca
    25381 gtttctcaaa ccaaattgtt aagggaattg aggatatcgc cagccagaat ggctaccagg
    25441 taatgatagg aaatagtaat tacagccaag agagtgagga ccggtatatt gaaagcatgc
    25501 ttctcttggg agtagacggc tttattattc agccgacctc taatttccga aaatattctc
    25561 gtatcatcga tgagaaaaag aagaaaatgg tcttttttga tagtcagctc tatgaacacc
    25621 ggactagctg ggttaaaacc aataactatg atgccgttta tgacatgacc cagtcctgta
    25681 tcgaaaaagg ttatgagcat tttctcttga ttacagcgga tacgagtcgt ttgagtactc
    25741 ggattgagcg ggcaagtggt tttgtggatg ctttaacaga tgctaatatg cgtcacgcca
    25801 gtctaaccat tgaagataag catacgaatt tggaacaaat taaggaattt ttacaaaaag
    25861 aaatcgatcc cgatgaaaaa actctggtat ttatccctaa ctgttgggcc ctacctctag
    25921 tctttaccgt tatcaaagag ttgaattata acttgccaca agttgggttg attggttttg
    25981 acaatacgga gtggacttgc ttttcttctc caagtgtttc gacgctggtt cagccctcct
    26041 ttgaggaagg acaacaggct acaaagattt tgattgacca gattgaaggc cgcaatcaag
    26101 aagaaaggca acaagtcttg gattgtagtg tgaattggaa agagtcgact ttctaaaatg
    26161 aaggaaaatg acttgcaatc tctgttaaga aataaaataa tcccacctag aacaagctag
    26221 gtgggattat ttgcctatga aatgagaaat tatgggagca agctcctaaa tcaactgttt
    26281 ttgatctact tctttaacta cttgataaaa gttatagaag taggccaaac ttgaaatgat
    26341 ggttacgact aggaatattg aaaatttcca ttggacaggg gtggttaaaa gttgtggaaa
    26401 ggatatgagg agaaagaaga gggctgcgtt gaggacaggt atccgttttg attgtatttt
    26461 ctcaagtcct ttattgagcg caggaagaaa gaggagtagg agtagtaaaa ctgtatgaga
    26521 aacagctcct gaaataaggg cgaagaaaag gaaaatactg ataaaaacat gaatgatcag
    26581 tagtctagct agtgatttca taaggcacct cctaatcctg gtctttttta gctcttgcaa
    26641 tacgaagtga gtcgacaata tgtatcatca ctccgaaaaa gaaagctccc agtatagttt
    26701 taaaaatatg ttttgtattt agaagagaac tgataaaatt tggattttca cttgttaggg
    26761 tatcaatgag tggaattata aaaaatatca ctgttccata aatcgaacct gctttcagac
    26821 caggataacg taactgtctc ttttcttttt tcatagggca cctcctaatc ctcatcttga
    26881 tttttcttag tttttgcaat gcgacgggag atgaggaact gtatgctcgc tccgaagaaa
    26941 atagaaccga gaatgcttga tacaccattt cttatagtga gaagagaatg aaaatagtcc
    27001 tgaccttcac ctatgagtat cctgagaaga ggagttataa aaaacatcca tagaccaaag
    27061 aacaaacctg ctttcagacc tgggtaatgt agttgcttac tttctttctc gctcagcatg
    27121 tctggatcaa tagctgtaat ccctgttttt ttcatttggt aggtgacata gccagcagcg
    27181 atgagggcaa tcactaaaat cagaggagga tagattagag ccacttcttg agggtattta
    27241 taggccagaa ggagtggaat aagatttccg aaaatcatca gataaaagag gatgataaag
    27301 acttggttcc caatacgatc ggcctcacgc cgtttgtatt cgtcaagggg accagaaata
    27361 ccgtatgtgc gtttgatcag tttttcagtg aaggtttctt ttttcatgag tttgctcctt
    27421 ttttaaaaat cttcctccca aaagagactg ttgaggtcag tttggaggct gcgggcgaga
    27481 ttgagacaga gttccaaggt tggattgtac ttgtcgtttt caatcatatt gatagtctgt
    27541 ctcgagacac tgatatcctt ggcgagttcg agctgggaaa tacccaattc cttgcgaaat
    27601 tctttcacac gattcatctg gtctcctttc tgatttatgt cgtatatatt tgactatatt
    27661 atagtctttt aaacataaag tgtcaagtat ttttgacata ttttttgaag aaatagtagt
    27721 ctccttgtcc tatttgtctg acaagtgcaa gctggtcgga tttgtggtaa aatagataag
    27781 atatgacaaa agaatttcat catgtaacgg tcttactcca cgaaacgatt gatatgcttg
    27841 acgtaaagcc tgacggtatc tacgttgatg cgactttggg cggagcagga catagcgagt
    27901 atttattaag taaattaagt gaaaaaggcc atctctatgc ctttgaccag gatcagaatg
    27961 ccattgacaa tgcgcaaaaa cgcttggcac cttacattga gaagggaatg gtgaccttta
    28021 tcaaggataa cttccgtcat ttacaggcac gtttgcgcga agctggtgtt caggaaattg
    28081 atggaatttg ttatgacttg ggagtgtcta gtcctcaatt ggaccagcgt gagcgtggtt
    28141 tttcttataa aaaggatgcg ccactggaca tgcggatgaa tcaggatgct agtctgacag
    28201 cctatgaagt ggttaatcat tatgactatc atgatttggt tcgtattttc ttcaaatacg
    28261 gtgaggataa attctctaaa cagattgcgc gtaagattga gcaagcgcgt gaagtgaagc
    28321 cgattgagac aacgactgag ttagcagaga ttatcaagtt ggtcaaacct gccaaggaac
    28381 tcaagaagaa gggtcatcct gctaagcaga ttttccaggc tattcgaatt gaagtcaatg
    28441 atgaactggg agcggcagat gagtccatcc agcaggctat ggatatgttg gctctggatg
    28501 gtagaatttc agtgattacc tttcattcct tagaagaccg cttgaccaag caattgttca
    28561 aggaagcttc aacagttgaa gttccaaaag gcttgccttt catcccagat gatctcaagc
    28621 ccaagatgga attggtgtcc cgtaagccaa tcttgccaag tgcggaagag ttagaagcca
    28681 ataaccgctc gcactcagcc aagttgcgcg tggtcagaaa aattcacaag taagagggaa
    28741 aaagatggca gaaaaaatgg aaaaaacagg tcaaatacta cagatgcaac ttaaacggtt
    28801 ttcgcgtgtg gaaaaagctt tttacttttc cattgctgta accactctta ttgtagccat
    28861 tagtattatt tttatgcaga ccaagctctt gcaagtgcag aatgatttga caaaaatcaa
    28921 tgcgcagata gaggaaaaga agaccgaatt ggacgatgcc aagcaagagg tcaatgaact
    28981 attacgtgca gaacgtttga aagaaattgc caattcacac gatttgcaat taaacaatga
    29041 aaatattaga atagcggagt aagatatgaa gtggacaaaa agagtaatcc gttatgcgac
    29101 caaaaatcgg aaatcgccgg ctgaaaacag acgcagagtt ggaaaaagtc tgagtttatt
    29161 atctgtcttt gtttttgcca tttttttagt caattttgcg gtcattattg ggacaggcac
    29221 tcgctttgga acagatttag cgaaggaagc taagaaggtt catcaaacca cccgtacagt
    29281 tcctgccaaa cgtgggacta tttatgaccg aaatggagtc ccgattgctg aggatgcaac
    29341 ctcctataat gtctatgcgg tcattgatga gaactataag tcagcaacgg gtaagattct
    29401 ttacgtagaa aaaacacaat ttaacaaggt tgcagaggtc tttcataagt atctggacat
    29461 ggaagaatcc tatgtaagag agcaactctc gcaacctaat ctcaagcaag tttcctttgg
    29521 agcaaaggga aatgggatta cctatgccaa tatgatgtcc atcaaaaaag aattggaagc
    29581 tgcagaggtc aaggggattg attttacaac cagtcccaat cgtagttacc caaacggaca
    29641 atttgcttct agttttatcg gcctagctca actccatgaa aatgaagatg gaagcaagag
    29701 cttgctggga acctctggaa tggagagttc cttgaacagt attcttgcag ggacagacgg
    29761 cattattacc tatgaaaagg atcgtctggg taatattgta cccggaacag aacaagtttc
    29821 ccaacgaacg atggacggta aggatgttta tacaaccatt tccagccccc tccagtcctt
    29881 tatggaaacc cagatggatg cttttcaaga gaaggtaaaa ggaaagtaca tgacagcgac
    29941 tttggtcagt gctaaaacag gggaaattct ggcaacaaca caacgaccga cctttgatgc
    30001 agatacaaaa gaaggcatta cagaggactt tgtttggcgt gatatccttt accaaagtaa
    30061 ctatgagcca ggttccacta tgaaagtgat gatgttggct gctgctattg ataataatac
    30121 ctttccagga ggagaagtct ttaatagtag tgagttaaaa attgcagatg ccacgattcg
    30181 agattgggac gttaatgaag gattgactgg tggcagaatg atgacttttt ctcaaggttt
    30241 tgcacactca agtaacgttg ggatgaccct ccttgagcaa aagatgggag atgctacctg
    30301 gcttgattat cttaatcgtt ttaaatttgg agttccgacc cgtttcggtt tgacggatga
    30361 gtatgctggt cagcttcctg cggataatat tgtcaacatt gcgcaaagct catttggaca
    30421 agggatttca gtgacccaga cacaaatgat tcgtgccttt acagctattg ctaatgacgg
    30481 tgtcatgctg gagcctaaat ttattagtgc catttatgat ccaaatgatc aaactgctcg
    30541 gaaatctcaa aaagaaattg tgggaaatcc tgtttctaaa gatgcagcta gtctaactcg
    30601 gactaacatg gttttggtag ggacggatcc ggtttatgga accatgtata accacagcac
    30661 aggcaagcca actgtaactg ttcctgggca aaatgtagcc ctcaagtctg gtacggctca
    30721 gattgctgac gagaaaaatg gtggttatct agtcggggta accgactata ttttctcggc
    30781 tgtatcaatg agtccggctg aaaatcctga ttttatcttg tatgtgacgg tccaacaacc
    30841 tgaacattat tcaggtattc agttgggaga atttgccaat cctatcttgg agcgggcttc
    30901 agctatgaaa gactctctca atcttcaaac aacagctaaa gctttggagc aagtaagtca
    30961 acaaagtcct tatcctatgc ctagtgtcaa ggatatttca cctggtgatt tagcagaaga
    31021 attgcgtcgc aatcttgtac aacccatcgt tgtgggaaca ggaacgaaga ttaaaaacag
    31081 ttctgctgaa gaagggaaga atcttgcccc gaaccagcaa gtccttatct tatctgataa
    31141 agcagaggaa gttccagata tgtatggttg gacaaaggag actgctgaga cccttgctaa
    31201 gtggctcaat atagaacttg aatttcaagg ttcgggctct actgtgcaga agcaagatgt
    31261 tcgtgctaac acagctatca aggacattaa aaaaattaca ttaactttag gagactaata
    31321 tgtttatttc catcagtgct ggaattgtga catttttact aactttagta ggaattccgg
    31381 cctttatcca attttataga aaggcgcaaa ttacaggcca gcagatgcat gaggatgtca
    31441 aacagcatca ggcaaaagct gggactccta caatgggagg tttggttttc ttgattactt
    31501 ctgttttggt tgctttcttt ttcgccctat ttagtagcca attcagcaat aatgtgggaa
    31561 tgattttgtt catcttggtc ttgtatggct tggtcggatt tttagatgac tttctcaagg
    31621 tctttcgtaa aatcaatgag gggcttaatc ctaagcaaaa attagctctt cagcttctag
    31681 gtggagttat cttctatctt ttctatgagc gcggtggcga tatcctgtct gtctttggtt
    31741 atccagttca tttgggattt ttctatattt tcttcgctct tttctggcta gtcggttttt
    31801 caaacgcagt aaacttgaca gacggtgttg acggtttagc tagtatttcc gttgtgatta
    31861 gtttgtctgc ctatggagtt attgcctatg tgcaaggtca gatggatatt cttctagtga
    31921 ttcttgccat gattggtggt ttgctcggtt tcttcatctt taaccataag cctgccaagg
    31981 tctttatggg tgatgtggga agtttggccc taggtgggat gctggcagct atctctatgg
    32041 ctctccacca agaatggact ctcttgatta tcggaattgt gtatgttttt gaaacaactt
    32101 ctgttatgat gcaagtcagt tatttcaaac tgacaggtgg taaacgtatt ttccgtatga
    32161 cgcctgtaca tcaccatttt gagcttgggg gattgtctgg taaaggaaat ccttggagcg
    32221 agtggaagat tgacttcttc ttttggggag ttgggcttct agcaagtctc ctgaccctcg
    32281 caattttgta tttgatgtaa gaatggcacc ctgatgtttc agggtgtttt tgtgtttaaa
    32341 tacacaatga aaatcaaaga acaaactaga aagctaactt taggctgctc aaaacatagt
    32401 agtttgaaac taaaatagta cacctctact tctaaaacat tgttagaaat cgatttgact
    32461 gtcctgatca atttgtcctg ttcttatttc attttaatat acagttttga gatggttgat
    32521 agaactgacg aagtcagctc aaaacacggt tttgaggttg tagacaaggc gaagctgatg
    32581 tggtttgaag aggttttcga agagtgtaaa aaaacattgg tcagttaaag tcaaaaatat
    32641 tgaccttttc tgaccaatgt agtatattat gaacgtaagg taaatgaaag ccttactaga
    32701 acacttattt aaagtaaaat agaaagaggt gagtctatgt ttcatctaga aatcttcaga
    32761 agtaaagata gtctactcct gcttgaaaaa gaaaaaccag aaatagtacg tagggtagcg
    32821 gtttagctag tctaaatttt ttaaaacaaa ggtcaaagat agtcaatgtc agtaatcata
    32881 actaagtaaa caaaaagagg taaagaatat gaataacaac tttaataatt ttaataacat
    32941 ggatgattta tttaaccaat tgatgggtgg tatgcgagga tatagttctg aaaatcgccg
    33001 ttacttaatt aatggacgcg aagtcacacc tgaggaattt gctcactatc gtgcgactgg
    33061 tcaattacca ggaaatgcag aaactgatgt gcaaatgcca caacaggcat caggtatgaa
    33121 acaagacggt gtccttgcaa aactaggtcg aaacttgaca gcagaagcgc gtgagggcaa
    33181 gttggatcct gttatcggac gaaacaagga aattcaagaa acatctgaaa tcctctcacg
    33241 ccgcaccaag aacaatcctg ttttggtcgg agatgcaggt gttggtaaga cagcagttgt
    33301 cgaaggtcta gcgcaagcca ttgtgaacgg agatgttcct gctgctatca agaacaagga
    33361 aattatttct attgatatct caggtcttga ggctggtact caataccgtg gtagctttga
    33421 agaaaatgta caaaacttag tcaatgaagt gaaagaagca gggaatatta tcctcttctt
    33481 tgatgaaatt caccaaattc ttggtgctgg tagcactggt ggagacagtg gttctaaagg
    33541 gcttgcggat attctcaagc cagctctctc tcgtggagaa ttgacagtga ttggggcaac
    33601 aactcaagac gaataccgta acaccatctt gaagaatgct gctcttgctc gtcgtttcaa
    33661 cgaagtgaag gtcaatgctc cttcagcaga gaatactttt aacattcttc aaggcattcg
    33721 tgacctctat caacaacacc acaatgtcat cttgccagac gaagtcttga aagcagcggt
    33781 ggattattct gttcaataca ttcctcaacg tagcttgcca gataaggcta ttgaccttgt
    33841 cgatgtaacg gctgctcact tggcggctca acatccagta acagatgtgc atgctgttga
    33901 acgagaaatc gaaacggaaa aagacaagca agaaaaagca gttgaagcag aagattttga
    33961 agcagctcta aactataaaa cacgcattgc agaattggaa aagaaaatcg aaaaccacac
    34021 agaagatatg aaagtgactg caagtgtcaa cgatgtggct gaatctgtgg aacgaatgac
    34081 aggtatccca gtatcgcaaa tgggagcttc agatatcgaa cgtttgaaag atatggctca
    34141 tcgcttgcaa gacaaggtga ttggtcaaga taaggccgta gaagctgtag ctcgtgctat
    34201 ccgtcgtaac cgtgctggtt ttgatgaagg aaatcgccca atcggcagct tcctctttgt
    34261 agggtctact ggggttggta agacggagct tgctaagcaa ttggcactcg atatgtttgg
    34321 aacccaggat gcgattatcc gtttggatat gtctgaatac agtgaccgca cagctgtttc
    34381 taagctaatt ggtacaacag caggctatgt gggttatgat gacaatagca ataccttaac
    34441 agaacgtgtt cgtcgcaatc catactctat cattctcttg gatgaaattg aaaaggctga
    34501 ccctcaagtt attacccttc tcctccaagt tctagatgat ggtcgtttga cagatggtca
    34561 aggaaataca gtaaacttca agaacactgt cattattgcg acctcaaatg ctggatttgg
    34621 ctatgaagcc aacttgacag aagatgcgga taaaccagaa ttgatggacc gtttgaaccc
    34681 cttcttccgt ccagaactcc tcaaccgctt taatgcagtc atcgagttct cacacttgac
    34741 taaggaagat ctttctaaga ttgtagattt gatgttggct gaagttaacc aaaccttggc
    34801 taagaaagac attgacttgg tagtcagtca agcggctaaa gattatatca cagaagaagg
    34861 ttacgacgaa gtcatggggg ttcgtcctct ccgtcgagtg gttgaacaag aaattcgtga
    34921 taaggtgaca gacttccact tagatcattt agatgctaaa catctggaag cagatatgga
    34981 agatggcgtt ttggttattc gtgagaaagc ctaagataga attttgagga taaaaaagaa
    35041 ggagccagct gaaaaaaact ggttcctttt gaggtacgac gggcatgtcg tatagtagaa
    35101 gtgtactatt ctagtttcaa tatactatag tagctcagaa gtcgatactt aaacgtgcta
    35161 tatcaaaacc agtccttgaa aaacgcggac tggtttcgtg tttggattat taccttgaac
    35221 gacatgcgtt aaaagttagt tgaaccgccg tatgcctaat ggtacgtacg gtggtgtgag
    35281 aggggctaga gattatcccc tactcgattt taaatcacat gacgttcaaa ggcatcatct
    35341 gaaatccctt gttccaagat gagttttgcc cattctttag cagagaagag gctgtggtcc
    35401 ttgtagtttc cgcaagattc gatggttgtc cctgggacat cttcccaagt agtagtttca
    35461 gcgatttcct tgagcgaatc cttgataaca gctgcgattt tagcactggt gtgacgtccc
    35521 cacataatca tgtggaagcc tgtgcggcaa ccaaatggtg aacagtcaat catgccgtca
    35581 atgcgggtac ggatgagttt ggctaagagg tgctcgatag tgtgaaggcc ggcagtaggg
    35641 atagagtctt cgtttggttg caccaagcga atatcataat tggagatgat gtctcctttt
    35701 ggtcctgttt cttccccaat caagcgaaca tagggtgctt tgacaatggt gtggtcaagt
    35761 tcaaaacttt cgacaataac ttcttttgac atggtaaatc ctttcagttt tcttctctca
    35821 ttatatcata aaggttgctc ctgagacaga gagaaaacct ctccgaggct ggagaggttg
    35881 aaatctttac ttacgatata agcggtcgta ttggtagtat gggtcaaagg ttacgttgat
    35941 acctagttta cgaaggacat tcttgtcttc atcagtcaag atgatggttg agtgggcttc
    36001 gcttcctttg aggttgccga gttcttccat agcgcgggca gcatcaggat tttctgtagc
    36061 tgtgatagca agtgcaatca ggatttcatt tgaatgaagg cgtggattgc ggctaccgag
    36121 atgatcgatt ttaagacctt ggattggctt aacaacttca ggctcgatta gttttacttc
    36181 tttagcgatg tcagctgatt ttttgatggc gttgatcaag gcagcggctg taggcccaaa
    36241 gagttctgag ttcttaccag tgacgatttc cccatttggc aattcaaagg ctagggctgg
    36301 tccaccagtt tcttctgctt tttggcgcgc aacgacagca accttacggt ctgcaggtgt
    36361 gataccgagg tcgttcatga gcaactcaat tttcttgacg gcagcttcgc caactttttc
    36421 agctttgaag tcaagaactg tttgatagta acggcggatg atttcttgtt tagaggcttc
    36481 gacagcagct tcgtcatctg taatagcgaa accaaccatg ttgacaccca tatctgtcgg
    36541 tgaagcgtat ggtgattttc cgagaatacg ttccaacatg cgcttgagca ctgggaagat
    36601 ttcgatatca cggttgtagt tgacagtggt ttctctatag gtttgaagat gaaaggggtc
    36661 aatcatgttg acatcatcaa ggtcagctgt ggcagcttca taagccaagt taactggatg
    36721 atgaagggga agattccaaa cagggaaggt ttcaaatttt gcgtagccag atttgatgcc
    36781 attgatttgg tcgtggtaca tattggacat acacgttgcc aattttccag aaccgggtcc
    36841 aggagcggtt acgacaatca agttgcgact ggttttgatg tagtcgtttt tgcccatgcc
    36901 ttctggggaa atgatgtgat ccatatctgt cggatatcct ttgattggat aatgaagata
    36961 agaatcaatt ccgtttttct caagttggtt gcggaaggca tctgcagcga gttggccagc
    37021 gtattgtgta atgacaacgg agctgacaaa aatccccaat tcattgaatt tatcaatcaa
    37081 acgaagaact tcttggtcat aagaaatgcc taagtcgcca cgtgctttgg aatgttcaat
    37141 gttgctagca ttaatggcaa tcacaacctc aacctgctct ttcaattctt gcaagagctt
    37201 gattttgttg tcaggttcat aaccaggaag gacacgagca gcgtggaaat cttctaacat
    37261 tttaccgcca aactctaagt agagcttgcc gtcaaattgg ttaatgcgct ccaaaatatg
    37321 gtcgcgttgt aaattcaaat attgttcaga actaaaagct tgttttttca tttttttacc
    37381 tctggactct attataataa aaaattggaa gttaggaaac tacggagcta aaaaagaaat
    37441 taaaaagatt aagcaaacgc ttgcacaaaa ttttaaaaag tgctatcata gactatagat
    37501 tatgaaaata atgaggtaaa cagatgcaag aaaaatggtg gcataatgcc gtagtctatc
    37561 aagtctatcc aaagagtttt atggatagta atggagatgg aattggcgat ttgccaggaa
    37621 ttaccagtaa gttggactat ctagccaagc taggaatcac agcaatttgg ctttctcccg
    37681 tttatgacag ccctatggat gataatggct acgatattgc tgattatcaa gcgattgcaa
    37741 cgatttttgg aaccatggag gatatggatc aactgattgc agaagctaag aagcgtgata
    37801 tccgtatcat catggacttg gtggtcaatc atacctcgga tgagcatgcc tggtttgtag
    37861 aggcctgtga aaatcctaat agccctgagc gagactacta tatctggcgc gatgaaccca
    37921 atgacctaga ttctatcttt agtgggtctg cttgggaata cgatgaaaag tcaggtcaat
    37981 actatctcca ctttttcagc aagaaacagc cggatctcaa ctgggaaaat gaaaaacttc
    38041 gccagaaaat ttatgagatg atgaacttct ggattgataa aggtattggt ggtttccgta
    38101 tggatgttat tgacatgatt ggcaaaattc ctgacgagaa ggtagtcaat aatggtccta
    38161 tgctccatcc ctatctcaag gaaatgaatc aggcgacctt tggagataag gatctcttga
    38221 cagtagggga gacttgggga gcaacgccag agattgccaa gctctactct gatccaaagg
    38281 ggcaagaatt gtctatggtc ttccagtttg aacatatcgg tcttcagtat caggaaggtc
    38341 agcctaaatg gcactatcaa aaagagctga atatcgctaa gttaaaagaa atcttcaaca
    38401 aatggcagac agagttagga gttgaggacg gctggaattc cctcttctgg aacaaccatg
    38461 acctccctcg tattgtctca atctggggaa atgaccaaga ataccgcgaa aaatctgcca
    38521 aagcctttgc aatcttactt catctcatga gaggaactcc ttatatctac caaggtgagg
    38581 agattgggat gaccaactat ccgtttgaaa cactggatca agtagaagat attgaatctc
    38641 tcaactatgc gcgtgaggct cttgaaaaag gtgttccgat tgaagaaatc atggacagta
    38701 tccgtgttat tggacgtgac aatgcccgta cccctatgca atgggacgag agcaaaaacg
    38761 ctggtttctc aacaggtcaa ccttggttgg cggttaatcc aaattacgag atgatcaacg
    38821 tccaagaagc gctggcaaat ccagattcta ttttctatac ctatcagaaa ctggtccaaa
    38881 ttcgcaagga gaatagctgg ctaattcgag ctgactttga attgcttgat acggttgata
    38941 aggtctttgc ttatatacgt aaggatggcg gccgtcgctt cctagttgtg gctaacttgt
    39001 ccaatgaaga gcaagacttg acagtagaag gaaaagtcaa atctgtcttg attgaaaaca
    39061 ctgcggctaa agaagtactt gaaaaacagg tcttggctcc atgggatgct ttctgtgtgg
    39121 aattactata aatatttttt gcagaaaaat ttaaaattga aatcatataa aaacaaggga
    39181 ggactgtata aaaagcagaa atcctttgtt ttttataacc aaggtttata aactttcatt
    39241 ctcgaaattc aattaacttt acaaattccc actactaagg agaaagaaga tgaacataaa
    39301 gaagcgtgtc cttagtgcag gtctgacttt tgcatctgct ttgcttttag ctgcttgcgg
    39361 ccaatcaggt tcagatacaa aaacttactc atcaaccttt agtggaaatc caactacatt
    39421 taactatcta ttagactatt acgctgataa tacattgatt atcaccaatc tagttgatgg
    39481 tttgcttgaa aatgacaatc acggaaacct agttccatct ttgccagagg actggtctgt
    39541 ttcgagcgac ggtctgactt atacttacaa attgagaaaa gatgccaaat ggttcacagc
    39601 tgacggtgaa gagtactccc cagtcaaggc ccaggatttt gtgacaggta tcaagtacgt
    39661 agtggataat aaatcacagg ctattgactt gattcaaaac tcgatcaaag gtttgaatga
    39721 ttatattaca ggagcggatt ctgacttttc taaggttggg atgaaggcca ttgacaacca
    39781 gactgttgag tatactttgg tacgcccaga accttactgg aactcaaaaa caaccaacag
    39841 tattcttttc ccggtaaact aagagtttct aaattcaaaa gggaaagatt ttgggacctt
    39901 atctccagat agtattctct acagcggtcc ttatttgtta aaagatttca catcaaaatc
    39961 atttatcgag tatgtgaaaa acccacatta ctatgatcat gccaaagtat cgattgaaca
    40021 cgtgaaattg gcttacttta atggatcaga tcaagaattg actatccgta actttgaaag
    40081 tggagcttat tctatcgctg gagtttatcc aaatagttcg aactttgcta agaccaagga
    40141 gaaatataag gataatatcg tctatagctt gcaggacaag acttcttggt atttaaattt
    40201 caatgtcaat cgcgaggctt gcaatcacac tactaaaacg acagatgagc agaagaagtc
    40261 aactgagaca gctgtcttga acaaaaactt ccgccaagca gtaaactttg ccttggaccg
    40321 cacagcccat tctgcccagt caaatgggga agaggcggct agcaagaccc ttcgtaacac
    40381 cttggtgctc ctacatttgt ccaagttgga gacaagacct ttggagaagt agttgcttct
    40441 aaattggtca actatggtac agaatggagg tattgacttg gcagatgctc aggatgccta
    40501 tttcaacaaa gaaaaagccc aagcaaaatt tgcggaagct aaaaaagaat tggcaagtca
    40561 aggtgtgact ttccctattt acgtgaatgt ggcagtagat cagacaagta aaaatgccgt
    40621 tacaggcatg aactcagtta agcagactct ggagtcagtt ttaggtgctg ataacattgt
    40681 tattgatgtt cagcaacttt caacagatga ttttaataat gtaccttctt ggcaccgatg
    40741 ccagctgata gaggctatga tttgagcttt gatggttggg tgggtgacta tcaggatcca
    40801 tcaacttatc tcaatccttt caatgcagag aatggcttct atctcaaaat ttttggaccg
    40861 gatgccaagg aagataagga aaaaatcact agcctaggtc ttgataccta cactaagatg
    40921 ctcaaagatg cagatagtga aaacaaagat gtagccaaac gctatgaaaa atatgctgaa
    40981 gcacaggctt ggatgattga caattctctc attatgtcag ctatgtcaag tggtggcaca
    41041 gcatctgtaa ccaaagtgat gccatttaca agagggcatt aattggttgg tattaagggt
    41101 gatggcaata actacaagta catgaaactg caaaaagata ctgtaacaac caaacggttt
    41161 gaaaaagcta agaccaaatg ggagcaagaa agcaaaaaag caatcgaaaa agcccaaaaa
    41221 gaagcagaaa aacatgttaa atagttataa atagcttttt caaggagaat tataaagaca
    41281 aacatcagtt ttggtgcttg tctttttaaa tcactcggtt attgaaaatt agacttcttg
    41341 cgaaacaggc tctttgtcaa ctgtagtggg ttgaagaaaa gctaagctcg agaaaggaca
    41401 aatttcgtcc tttctttttt gatgttcaaa gtgataa
//
LOCUS       DAVDWM010000013        39036 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVDWM010000013 DAVDWM010000000
VERSION     DAVDWM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.013"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(162..1466)
                     /gene="eno"
                     /locus_tag="VUW49_000874"
     CDS             complement(162..1466)
                     /gene="eno"
                     /locus_tag="VUW49_000874"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7980817.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFLGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            1630..2076
                     /locus_tag="VUW49_000875"
     CDS             1630..2076
                     /locus_tag="VUW49_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET7980818.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2073..3188)
                     /locus_tag="VUW49_000876"
                     /pseudo
     CDS             complement(2073..3188)
                     /locus_tag="VUW49_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(3197..3430)
                     /locus_tag="VUW49_000877"
     CDS             complement(3197..3430)
                     /locus_tag="VUW49_000877"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET7980819.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAKLG
                     IAFCAKEVLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            complement(3471..3842)
                     /locus_tag="VUW49_000878"
     CDS             complement(3471..3842)
                     /locus_tag="VUW49_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET7980820.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(3901..5334)
                     /gene="glgA"
                     /locus_tag="VUW49_000879"
     CDS             complement(3901..5334)
                     /gene="glgA"
                     /locus_tag="VUW49_000879"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET7980821.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLASIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5331..6470)
                     /gene="glgD"
                     /locus_tag="VUW49_000880"
     CDS             complement(5331..6470)
                     /gene="glgD"
                     /locus_tag="VUW49_000880"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET7980822.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6460..7602)
                     /locus_tag="VUW49_000881"
     CDS             complement(6460..7602)
                     /locus_tag="VUW49_000881"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET7980823.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(7592..9520)
                     /gene="glgB"
                     /locus_tag="VUW49_000882"
     CDS             complement(7592..9520)
                     /gene="glgB"
                     /locus_tag="VUW49_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET7980824.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSAIKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTITNKNQKGVENEK"
     gene            complement(9906..11330)
                     /locus_tag="VUW49_000883"
     CDS             complement(9906..11330)
                     /locus_tag="VUW49_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7980825.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGSFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     SLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(11874..14153)
                     /gene="pulA"
                     /locus_tag="VUW49_000884"
     CDS             complement(11874..14153)
                     /gene="pulA"
                     /locus_tag="VUW49_000884"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET7980826.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEIYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(14265..16223)
                     /gene="ligA"
                     /locus_tag="VUW49_000885"
     CDS             complement(14265..16223)
                     /gene="ligA"
                     /locus_tag="VUW49_000885"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7980827.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSCEELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(16456..18090)
                     /locus_tag="VUW49_000886"
     CDS             complement(16456..18090)
                     /locus_tag="VUW49_000886"
                     /inference="COORDINATES: protein motif:HMM:NF022633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HET7980828.1"
                     /translation="MVEFRYFNKEYKNVADKTFSESIVFFKDNWDDYGYYITFHVYCY
                     DKECGERYIGSYRIYESEIEQQENSDGIKSIFKLSKDDFDRNQKYSLACNLEFYQNLY
                     KFCSEYYNDFLERNKDLTLIDLPEEIKENKGVKKALLRNDGITKSAEIINLNQRLKEI
                     DENLNIGNFSEDDSVTTIFEKFIKDETFERYERLFNHIKLFNTWSKDYLTPILEKIIE
                     KIKNSGNNQQVNEEQLKCFIEYLDKNTTNLVHNLVSDEEHIRKIKEAVKCIKDFLRID
                     LENFQKFSADYNLVHYTSLSTLDILVKKSQNTADDYSRLRLSNARQLNDPTEGTVFLK
                     QVGFDLHTLLQLDYEPTNIFLSSMSKSSNENGLSDSLPMWKQYADNATGICLTYDRNY
                     LKSLIKKKSDTDDNDSKVEFYRVCYTKELESDDNKVILDQIEIIKKELKKIYLKDSQS
                     RILSEFDIVRYLFKESKYEYEQEYRLIKECNDDEIQIEKYGEMQVPRLFTYLSENLKY
                     SKITLGPKCDDIDYVAPFIKYVDHNIEVTKSQIPYR"
     gene            complement(18104..20005)
                     /locus_tag="VUW49_000887"
     CDS             complement(18104..20005)
                     /locus_tag="VUW49_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7980829.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSAFYLGDYDYYIEKKVEVEMSQIEEASTNNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
     gene            complement(20433..20708)
                     /locus_tag="VUW49_000888"
     CDS             complement(20433..20708)
                     /locus_tag="VUW49_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7980830.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(20814..21653)
                     /locus_tag="VUW49_000889"
     CDS             complement(20814..21653)
                     /locus_tag="VUW49_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7980831.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKNHELQPMVKGRGTKTFKKWLDELITS
                     LSERTVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            21795..22643
                     /locus_tag="VUW49_000890"
     CDS             21795..22643
                     /locus_tag="VUW49_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET7980832.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(22736..23653)
                     /locus_tag="VUW49_000891"
     CDS             complement(22736..23653)
                     /locus_tag="VUW49_000891"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET7980833.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            24091..24296
                     /locus_tag="VUW49_000892"
                     /pseudo
     CDS             24091..24296
                     /locus_tag="VUW49_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1980 domain-containing protein"
     gene            complement(24607..24900)
                     /gene="rpmA"
                     /locus_tag="VUW49_000893"
     CDS             complement(24607..24900)
                     /gene="rpmA"
                     /locus_tag="VUW49_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7980834.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(24917..25261)
                     /locus_tag="VUW49_000894"
     CDS             complement(24917..25261)
                     /locus_tag="VUW49_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET7980835.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(25277..25591)
                     /gene="rplU"
                     /locus_tag="VUW49_000895"
     CDS             complement(25277..25591)
                     /gene="rplU"
                     /locus_tag="VUW49_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7980836.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLIG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(25628..25715)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(25810..26409)
                     /locus_tag="VUW49_000896"
     CDS             complement(25810..26409)
                     /locus_tag="VUW49_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET7980837.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDIR
                     DKLLTLIAFMMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSVYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            26880..27179
                     /locus_tag="VUW49_000897"
     CDS             26880..27179
                     /locus_tag="VUW49_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7980838.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVISIDFNPAVIDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            complement(27520..28494)
                     /gene="pta"
                     /locus_tag="VUW49_000898"
     CDS             complement(27520..28494)
                     /gene="pta"
                     /locus_tag="VUW49_000898"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET7980839.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(28538..29434)
                     /locus_tag="VUW49_000899"
     CDS             complement(28538..29434)
                     /locus_tag="VUW49_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7980840.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(29431..30249)
                     /locus_tag="VUW49_000900"
     CDS             complement(29431..30249)
                     /locus_tag="VUW49_000900"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET7980841.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(30233..30904)
                     /locus_tag="VUW49_000901"
     CDS             complement(30233..30904)
                     /locus_tag="VUW49_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET7980842.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            31032..31601
                     /locus_tag="VUW49_000902"
     CDS             31032..31601
                     /locus_tag="VUW49_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET7980843.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            31714..32673
                     /locus_tag="VUW49_000903"
     CDS             31714..32673
                     /locus_tag="VUW49_000903"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET7980844.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            32683..33798
                     /locus_tag="VUW49_000904"
     CDS             32683..33798
                     /locus_tag="VUW49_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7980845.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFVNNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            33803..34150
                     /locus_tag="VUW49_000905"
     CDS             33803..34150
                     /locus_tag="VUW49_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET7980846.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            34158..34373
                     /locus_tag="VUW49_000906"
     CDS             34158..34373
                     /locus_tag="VUW49_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET7980847.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            34456..35097
                     /locus_tag="VUW49_000907"
     CDS             34456..35097
                     /locus_tag="VUW49_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET7980848.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            35115..35804
                     /locus_tag="VUW49_000908"
     CDS             35115..35804
                     /locus_tag="VUW49_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7980849.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(35801..36481)
                     /gene="radC"
                     /locus_tag="VUW49_000909"
     CDS             complement(35801..36481)
                     /gene="radC"
                     /locus_tag="VUW49_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7980850.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(36524..38815)
                     /gene="pcrA"
                     /locus_tag="VUW49_000910"
     CDS             complement(36524..38815)
                     /gene="pcrA"
                     /locus_tag="VUW49_000910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET7980851.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNSWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
BASE COUNT        11805 a         8302 c         6928 g        12001 t
ORIGIN      
        1 aaagttatga aaaagatttt ttaaaaagtt ttgtcccttt tttgccctct aaatacaaaa
       61 atagcccttc ggataaaatc cgaggggcta gaaacgttgt taaatcaacg gccgaacttt
      121 tgaatttcat ggttcgggat aaaatagttc actgaactat tttatttttt aaggttgtag
      181 aatgatttca atccacgata ttcagctact tcaccaagtt ggtcttcgat acgaagcaat
      241 tggttgtatt tagcgatacg gtctgtacgt gaaagtgaac cagtcttgat ttgtcctgcg
      301 ttagttgcaa ctgcgatatc agcgattgtt gaatcttcag tttcacctga acggtgtgat
      361 acaacagcag tgtaaccagc ttctttagcc atttcgatag cttcaaaagt ttcagtaaga
      421 gtaccgattt ggttaacttt gataaggatt gagttagcag caccttcttg gataccacgt
      481 gcaaggtagt cagtgtttgt tacgaagaag tcgtcaccaa caagttgtac tttcttacca
      541 agacgttcag taagagcttt ccaaccatcc cagtcgtttt catccatacc atcttcaata
      601 gtgatgattg ggtatttgtt aaccaattct tcaaggtagt cgatttgttc tgcagatgta
      661 cgaacagcag caccttcgcc ttcaaattta gtgtagtcgt aaactttacg ttctttatcg
      721 tagaattctg atgaagcaca gtcaaatccg agaaatacgt ctttacctgg tacatatcca
      781 gcagcttcaa tcgcagcaag gatagtttca acaccatctt cagttccttc gaaacgagga
      841 gcgaatccac cttcgtcacc tacggcagtt tccaaaccac gtgatttaag gattttctta
      901 agagcgtgga agatttcagc accgtaacga agggcttctt taaatgttgg cgcaccaact
      961 ggcaagatca tgaactcttg gaaagcgatt ggagcgtcag agtgagaacc accgttgatg
     1021 atgttcatca ttggagttgg aagaacttta gtgttgaatc caccaaggta gctgtaaagt
     1081 gggatttcaa ggtagtcagc agcagcacga gctacagcga tagacacacc gaggattgca
     1141 ttcgcaccca atttaccttt gttaggagta ccgtcaagtg cgatcatagc acggtcaata
     1201 gcttgttgat cacgtacatc gtagccaatg atagcttcag caatgatgtt gtttacgttg
     1261 tcaacagctt tttgtgtacc aagaccaccg taacgagatt tgtcaccgtc gcgaagttca
     1321 actgcttcgt gttcaccagt agaagctcct gatggaacca taccacgtcc gaaagcacct
     1381 gattcagtgt aaacttctac ttcaagtgtt gggttaccgc gtgagtctag gacttcgcga
     1441 gcgtaaacat cagtaataat tgacattttt tactctcctt atgagttaaa ttttttacac
     1501 ctctataata ccttaaaacc cctccttttt caagaaaaaa cgttatcttt gtgcaacttt
     1561 tccttaactt tataaagtaa tcgctttctt ttgtctgttt tattctaact tttatgatat
     1621 actgttttca tgacagattt atcaaaacaa ttacttgaaa aagctcatgg tgggttaaaa
     1681 ataaatccgg atgagcaaag acgctatctt ggtacttttg aggaaagagt tcttggatat
     1741 gtagatattg acacagcaaa tagccctcag ttagaaaaag gctttttatt tattttagaa
     1801 aaccttcagg aaaaagcaga gccactattt gtgaagattt caccaactat cgaatttgat
     1861 aagcaagttt tctacttaaa agaagcaaaa gaaactgata gtcaagccac catagtatct
     1921 gaagagcata ttacttctcc ttttggcctg gttattcata gcaatgcacc agttcaagta
     1981 gaagaaaaag accttcgact tgcttttcca aaactttggg aagttaaaaa ggaagaacca
     2041 gccaaaacat ccttatggaa gaaatggttt agctaaatct tgcacatatt taataagtgc
     2101 ccaatattgg cagccgtgcg ctccagatag agactggcat ttttcaaact atcttctaaa
     2161 ggttcacttt tctccaaaat agaaaagaca gcttggatat tttcaaatgg taggggaggt
     2221 aaatcttcag caagactacc gcaaatagca ataacaggaa ctccaacagg ggttcttttt
     2281 gcaacaccta taggcgcttt cccagcaaag ctttgactat caagtcttcc ttctccaaca
     2341 acaaccaagt cagcatctga aactttctta tcaaagttga ttaagtccaa gcaggtatca
     2401 attccagaca cgatacttgc ctgagcaaag gcacacaaac caccagcaag gcctccacct
     2461 gctcctgctc ctttaatttc taatgttgca ggtgagaatt tttcataaaa atcttggatc
     2521 gcctgatcta cgactgcaaa catagtcgga tgtagacctt tttgattgcc aaaagtgtaa
     2581 gtcgcacctt gatgaccaca taagggactc acgacatctg ctaaaatatg aatttgaaca
     2641 ccttcaggaa ttttatagca attttatgtt gaaacagaag ctaagtttaa taaggattga
     2701 ccggaagcag gcaagacatt tccatcccta tcataaaatt gataacctaa accagcagca
     2761 atccccagtc ctccatcatt actggccgtg ccaccaacac cgatataaat atctttaatc
     2821 cctttagaaa tgagatggag aatcaactct ccaataccac aagtttggat ttgaagtgga
     2881 tttcgtttct ctagcggaat ttttccaaga ccaaccaagt cagctacttc aaatagtgcc
     2941 agttcccctt tttgaaaata gcgcatggct tctttttgtc caaaagggtc tgtcacttgg
     3001 atccattttt cttttaggtc aagagaatgt cggatagcat ctacagtacc ttctccccca
     3061 tcaccaacag ggcagaggag acattctaca tctgctatcg attgttggaa gcctcttttt
     3121 attgcttcag ctacctgttg agctgtcaag ctttccttaa acgaatccgg tgcaattaca
     3181 atcttcatat tttccctcat tctaaacagt caatcaaagg gagaacttct aaaaaatccc
     3241 tcttgtcaac atgatgtggt atttcttttt tgagcacttc tttggcacaa aaggcgattc
     3301 ctaacttcgc cgacttcaac attaatagat tattaacccc atcaccgatt gccaccgttc
     3361 tttctttaga aagttttagt ttctttctcc atttttccag agtctctttt ttgacctggg
     3421 gacttataat ttgtccaact aattttcctg ttaaaagacc ttctttgact tcaagctagt
     3481 tggcagtgaa ataggcaata ccaagggatt ttgctaatct ctccaactat tggtgtaaat
     3541 ccaccagaca ccagaccaac taggatgcca ttcttttgga gaatagagat gaactctggg
     3601 acatttagcg atagatgaat tgagttgaag acgttatcaa agaccaaaat aggaagacct
     3661 tccaacaagg acactctttt tcttaaactg ctttcaaaga ccaactctcc tcgcattgct
     3721 cgacttgtaa tctgcgaaat ttccgcctca tgacctgcct ctctccctaa aagatcaatc
     3781 acttcttcta ggattaaggt tccatctaca tccaaaacac acaagccttt tacttgagac
     3841 atcagttctc ctctctaaac agcctaaaaa tcgtatgaag tcatcatacg attttatcta
     3901 ttaattaact aaactatggt acaagtcaag gtatgacttg caggctgtat cccatgagaa
     3961 gtcactctcc atagcttgtt tttgtaggtt tctccaaatg tctggatggt ttctatacaa
     4021 gtccaatgct gtttggaaag tccaatttaa ccaataagga gatagattgt caaagctaaa
     4081 gccagtaccg cttccttcga ttggattgaa agcgcgaact gtatctcgca agcctccaac
     4141 ttcatggacc aatggcaagg ttccataacg catagccatc atttgagaca agccacacgg
     4201 ttcaaaacga cttggcatga ggaagaggtc acaagcagcg tagatttcct gagcaagttt
     4261 gacatcaaaa gtgatatttg ttgatagctt gtctgggtaa atctgagcaa accatgagaa
     4321 agctccttca aaggctggat cgccagttcc caaaagaaca atctgaacat cttcttgcaa
     4381 gatatggtga agactttcga ccaccacatc aaaacctttt tgacgtgtca aacgagaaac
     4441 aattcccacc agtggaacgt ctgctctaac aggcaagcca actctttctt gcaattttgc
     4501 cttatttttg gctttcccag acaaatcttc ctgattgaaa tgatagtcta aaagagcatc
     4561 cgtctgagga ttataaagat cagcatcaat cccattcacg ataccagata ctttaccaga
     4621 ctccatttta agaatctgat ccaaattaca tccaaactga ctagtcataa tttcatgagc
     4681 atagctaggt gaaacggttg aaacacggtt cgcatagaga atacctgcct tcatccagtt
     4741 cagacagttg ttccatcgaa gggtgccatc agcgtaacgt tcaaagccaa ctccaaacaa
     4801 atcacccaac attccttctg aaaattgtcc ttggaattct aaattatgaa tggttaaaac
     4861 tgtttcaatg tcctcatagg cttgaatcca acggtatttt tccttcaaca agaaaggaat
     4921 catagctgta tggtagtcat gaacatggag aagatcagga ataaagtcaa tcctttccat
     4981 agcctcaatg gaagccagtt ggaaaaaggc aaagcgttct ccgtcatcaa aatcaccgta
     5041 aacatgacca cggaagaaat aatattgatt gtcaataaag tagaaggtta caccatttaa
     5101 tactgttttc ttaattccac aatactgtct gcgccaacca acgctcacct caaaatgaag
     5161 cacatcttca atctgatttc caaatttagc ctctaccata tcatagtagg gtaaaatcac
     5221 tgcaacttcg tgcccagctt ttaccagtga ttttggaaga gcgccaatga cgtctcccaa
     5281 accacctgtt tttgaaaagg gtgcaccctc tgctgctaca aataaaattt tcatgaatga
     5341 atatcctctg ttactttagc acctttctta accacaactg gatgttctgc agttcctcga
     5401 atcacaacac caggctcaac ttcaacccct ttgtccaaga tagcatattc gacctgagcc
     5461 ccttctccaa taacaacacg agggaagagc aggctatctt taaccaagct atccttatgg
     5521 acatgaatat tacgtgatag aacagaatta gctacttgac cttcaataat actaccagag
     5581 gcaaactgag aagtgcttac cttagatgta ttagcatagt aagttggctc ttcgtttttg
     5641 acctttgtat aaatcttttg gtttggtgag aaaagagaat agaatttttg tgattcaagc
     5701 atatcgatat tcgcttgata ataagattta acagagtgaa tattggctag atagcccgtg
     5761 tactcgtagg cgaaagctcc ctcttttaca gccaaatccc gtaaaacata gcgcaatttc
     5821 tctggatgtt cttttttagc ttcttcttcc aagtgttcaa tcaaccaagg tgtatcaacg
     5881 acaaagatat ctgtagacat attgaacgtt tcagctgttg acttgctatc aaagagttta
     5941 tgagaaagaa catggtctgt ttcatctaca tccaagattg catttacttc tgaaatatct
     6001 ttcttagcta gttttttata aactacagtg ataggctctt ttgttgtact atgtaggtgg
     6061 aaaacttggt tcaaatcaat gttaataaga acatcacagt tgagggcaac cgtttggttt
     6121 gagccagaac gtttcaaata agtaagaagc tgttggtagt attcttttcc aactgtacta
     6181 ctttctacac gggtattgta aattcctaga tagtaatggc taagaagggt tgataagccc
     6241 cactcgcgtc ctgaacgaat atggtcaaat actgagctaa tattatcctg ctggaaaata
     6301 ccaaagacac tacgaacacc tgcattagca aggcttgaaa gtgggaagtc aatcaaacga
     6361 tatttcccac caaatggcaa acttgctact ggacggtggt ccgtcaatgt cgacatattg
     6421 tgaaaaccaa ctgtatttcc taaaatggca gaatatttat caatcttcat ctgttgctac
     6481 ccccactact tcattatatc ctacaacttg tacttcatct gttccatcaa tttcgacacc
     6541 gtcagaaata atcgcacctt caccaataat ggcacgttta atcttagctc cttgaccaat
     6601 gatagctcca ctcatgataa ctgaatcaag gacttccgct ccttcgcgaa cttgcgcgcc
     6661 tgttgaaagg atagaatgtt taacagttcc atcaacgaaa catccgtcta caactaatga
     6721 gtcttccaca tgagcatttg ccccgaggaa gtttggtggt gaaatcaagt ttcttgagta
     6781 aatcttccat tgacggttac gactatccaa ggcattttct ggagaaatat actccatgtt
     6841 cgcttcccaa agtgactcaa tagtaccaac atctttccaa taaccactaa attcgtaagc
     6901 ataaacactt tcacctgact caaggtaatt tggaatgaca tttttaccaa agtctgacat
     6961 gccaaccttg ctcttttcag cagcgactaa catattacga aggcgttgcc aatcaaaaat
     7021 gtagattccc atagaagctt ttgtagattt aggttgagct ggtttttctt caaattcaac
     7081 aatacgattg ttagcatctg tgttcatgat accaaaacgg cttgcttctt taagagggac
     7141 gtctaaaact gctactgtca agctggcatt attatcctta tgagactgga gcatatcatc
     7201 atagtccatt ttgtagatgt ggtccccaga caaaatcaag acatactcag gattgacact
     7261 gtcgatatag tcgatatttt ggtaaatagc gtgactagtc ccctcaaacc aacgatttcc
     7321 ttcacttgca gaataaggtt gaagaataga gacacctgaa ttaataccgt ctagtcccca
     7381 gcttgaacca ttcccaatat ggttgttgag agcaagtggt tgatactgtg taacaacccc
     7441 aacattatga atccctgagt tggcacagtt tgatagggca aagtcaatga tacggtagcg
     7501 cccaccaaat tgcacagctg gttttgcgat gctttgagtg agtttaccga gacgagttcc
     7561 ttgcccacca gcaagaatca aagctaacat ttcatttttc attttctact cctttttggt
     7621 ttttatttgt gatggtttta gtagatttca agcgacgttt gattttccat acacttgctc
     7681 ccatagccgg tagggtaaag gttaaggtct gctcataatc tttccatagg ccttcttgcg
     7741 tttgaacagt ttgattatgt tctttccaaa cgcctcccca ctcttccaac tcagtattcc
     7801 atacttcttc gtaaattcct gcaacgggta gtccgattgt aaaatctttc cgctcaacag
     7861 gtaccatgtt aaagatacag actaacattt ctcccttttt acccttacga ataaaggaaa
     7921 gaacactctg gtctcgatta tccgcatcaa tgatttcaat accatcatag ctggtatcga
     7981 tttcccacag acagcgatga tctttgtaaa actggtttag ctgagaagtg aaatacttca
     8041 tcttagcatt cattgggtct tctaggttag accattccaa ctgttcttca gatttccatt
     8101 ctaggaattg accgtattcg ctacccatga agagcaattt cttaccaggg tgacaaattt
     8161 ggtacgtata gagattgcgc aagcctgcga attgattgta acgatctccc cacatcttat
     8221 gcatcatact cttcttgcca tgaaccactt catcgtgcga gaatggcaag agataattct
     8281 ccttgaaaac atacataaag ctgaaagtca ccaggttaaa gtcatattta cgatagatcg
     8341 gatcttcttc gtagaaacgg aggatatcat tcatccagcc catgttccat ttgtagtcaa
     8401 atcctagacc accaatctct ttcattcccg taatcttgat cgcagacgaa ctttcttctg
     8461 caatcatcat cacatctgga tattctaact taataacctc attcaagcgc tgaaggaaat
     8521 aataaccttc atagttgaga tttccgccat ctttattagg tgtccatgga gcatcatcat
     8581 agtccaaata gagcatgttg ctaacagcat ccacacgaat accatccaaa tgatagacat
     8641 caatccaatg cttaatgcaa gaaattaaga aggactggac ttcatttttt ccaaggtcaa
     8701 aattaagggc accccaacca tggttatgag ccttattatg gtcttggtat tcaaaagtcg
     8761 gtgtcccatc ataataggct aaggcatcat cgttgatggt aaagtgacct ggtacccagt
     8821 ccacaataac cccaatatta tgggtatgac actcctcgac aaaatcttga aactcctctg
     8881 gtcggccata agcatgctct aaagcgaagt aacccataag ctgatacccc caactcaagc
     8941 ccaaaggatg ggacatcaag ggcataaact caatatgagt atagttcatt tcaacgagat
     9001 aaggaatgag ttcatccttg agctgggcaa aactataagg actgccatca gaatttcttt
     9061 tccatgatcc agcgtgaact tcataaatat tgacaggacg ctcttcaaag ccccaacgtt
     9121 ttcttcgtgc cagccaaagt ccatccttcc atttcttctc aggaagctct gttaagattg
     9181 cccctgttcc tggacgagcc tcatacctga cagcaaaagg gtcaatcttc atcaattgat
     9241 gaccattttg acgtgtgaca tgatatttgt aaatatgccc ttcttgagcc atattggtaa
     9301 agacttccca gaccccaaag tcatttctta ccattggaat ctgattttca atccagttgg
     9361 taaaatcacc aaccaagtga acagcctgag cattaggtgc ccaaacacgg aaggtatagc
     9421 catactctcc atttagttct tccctatgtg ctcctagata atgttggaga taaaaatttt
     9481 caccggtcat aaaggttttt aatgcttctc tattatccat atactccctt tctcttgtaa
     9541 gcgttttcta tgtttttatt atactacctt tttagagaag attcaagtaa attactatac
     9601 ttctttaatt attttgaaaa tctacaacaa gttcacttac tcgttcaatt gtaaatcaat
     9661 attttttcaa aaaattgcga aaacgccttt ctttttctac tatagtgaaa tatagtgaaa
     9721 tgaaataaaa catgcgcaag tcgattaagg aatttaatct aatttctaac aatgttttag
     9781 aagtagaggt gtactattct ggtttcaatc tattataata ccaaaaagtt cgtatttttt
     9841 ctataattat tattatttta tagcaaaaaa ataaaaccag gaaaacaatt tcctggtttt
     9901 acacgttatt tcacatcaaa aacaatggat ttgacatttg tcatcgcttc aatgctatat
     9961 ttaattcctt gcactccagc tccagaacct ttgacaccaa ggaatgggaa attatctgga
    10021 ccacgctggg ttttattatt aatgtggact gtacctactt caagtttttc agcaatttca
    10081 aaggcttttt tgaaatcatt tgtaaagact gatgattgaa ggccgaattc agattcgttg
    10141 gcaaaggcaa tagcctcctc tacactagcc acacgaatga ttggtaaaac aggaccaaat
    10201 ggctcttccc atgccacttt catatctttt gtaacttggt caaaaagcac tggccagaga
    10261 agattgccct cacgtttgat tggtgtaaga gcctgagcct ctttttcttg tgcatcctca
    10321 atcaagctcc aaatgaagtc ggctgaagca ttgtcaataa caggtgtaat atcagcattg
    10381 tcaaatggat caccaactgt taatttagaa acttcttcct gaagcaaagt agctaattta
    10441 tctgctacac tttcgagaac aatgacacgt ttaatggccg tgcaacgttg tcctgagtag
    10501 ctaaaggctc ccgcaacaat ttgcttggca gcatgttcca aatctgcatc ttctagtaca
    10561 agagctgcat ctttcccacc aagttccaac atgataggac gcataccagc taaacgacca
    10621 atacgttctc caataggagt tgaacctgta aagttgatga agttgacttc tttgtgctca
    10681 atgatataat ccccaatttc tgaaccacga cctgtaatgg tgttgaaaac acctgccgga
    10741 atccctgctt cttcaaatgc tttagccaac aagagtccag aaatggaacc ttgtgttggt
    10801 ggcttaaaca tgaccacatt ccctgcaatc aaggcaggtg caattttaga agcagataaa
    10861 ttaactggat aattaaaggg agcaatcgct agcacgatac caactggttc acgacggaca
    10921 acagccagtt tgtttttact tgctgcctca aaactaccac cttccattgc ttgtccagtg
    10981 atacggagac cttcctcagc agcataacga atcaagtctg ctgtacgcac tacttctcca
    11041 attgctgctt taatcccttt tgctacttct ttggcaagga tagtaccaat tttttcctta
    11101 tcgcgttcta aaatagctgc tgttttatgc aaataagccg cacgttcaat tgctgataaa
    11161 gctcgccatg ctggcagggc tgcacgcgca gcttgcatag cctcatcagc ttcagtctga
    11221 gtcatggctg gaactgtacc caattcttct tgattgattg gtgaataaat cgtaatttct
    11281 tgttcagatg atttccattt tccatttact aaattctgat atcttgtcaa attcttgtcc
    11341 tcctgaaaat gattaagata tattttacca aattttcaga aaatcacaac cctttttgca
    11401 aaatttttga gattattttc acaatcttga tttttcaaag tatactcaat aaaaataaaa
    11461 aaatccacta cgtcaaggcg aggctaatgt ggtttgaaga aattttcgaa gagcgtgaat
    11521 gagtatcatc tatagtaaaa taaaaaaact gaacaatttg gtttgggaca gtcaaaccaa
    11581 tttctcacaa tgttttagaa acacgggtgt gctattccaa tttcaggcta ctataactgt
    11641 catagattgc tgaaacaaag tctaggtaaa agtcttcata ataaaaagac ctcctatcaa
    11701 atgttcaaaa actttgatag gaggtcttgt ttcgtgaaaa tatttatcaa attttctata
    11761 caagtgagct gttagccagg ttctttctat tctttcaatt tcaatgaatg gattttttac
    11821 caatactcat aactgggaat ttttctgtgt aaaaatagcg agatagatgg tatttataaa
    11881 acactcaaga cagctagact aatatcattt aaaacattat cttcttttga gcgactgttg
    11941 gttaccaaca tagctaaatt tcctgcattt tcaaattgat agggttctga tttagcattc
    12001 acaactacca agaggtgttc tttgccgtga acttcataga taaggcagcc gctatgttca
    12061 atcgcagaat gcacaaagac atgatggtaa atttcatcat agctagagta agaaaaggca
    12121 ccagtttttg tcttcaatcg gatgacttga cggataaact caatactgtc ttgacgctca
    12181 ttaatcaagt tccagttcac ttggttcaca ctgtcaggag cattatagct attcatcgca
    12241 cgctctctat catcatgggt caactcacca ttttcaccag tcgcaaccag tttggtacga
    12301 ccaaattctt gaccgatttc cataaaggcc atcccctgca tgagcagatt catggctgtg
    12361 gcagtttcga ccttgcgcat gatttgctct gaactttggt ctggatgaag ggttgccaat
    12421 aaatcgtgaa gattgtaatt gtcatgggct tctacatagt taagcacctg atttggatgt
    12481 gtatagcttc ctaattcacg acttcctagg attgctttag ctagaattgg ctctgtcgca
    12541 gcaccactga caaaacctga cttgatagca ccataaattt ctcccccttt gacagcatcg
    12601 cgctgattgt cattaaagaa accaatattt ggcatctggt aggcattgtc cttcttggcc
    12661 ttatcataag gggcaagacc tgttcccata tcccatcctt ctccatagag gataatgttg
    12721 gagtcgattt catccaagct ttgacgaatc atctgcatgg tcttgacatc atgaatcccc
    12781 atcaagtcaa aacggaagcc gtcaatatta tattcctgca cccagtatag aagagaatca
    12841 atcatatact tgcgaaacat ttcgtgttca ctggctgttt catttccaac acccgttcca
    12901 ttctggaagg taccatctgg attcatacga taatagtaat cagggactgt tgtttggaat
    12961 ggtgcatcaa caactgagaa ggtatggtta tagactacat ccataatgac tccaataccc
    13021 gcatcgtgat aagcttgaac catcaccttc aaatcacgaa tgacctgagc tggatcatct
    13081 ggattagttg aaaaactagt ttctggcgcg ttatagtttt gtggatcata accccagttg
    13141 taggttacat ttccatcctc atcgtattct ttatgacggt ctgcaattgg ttgcaattga
    13201 acataattgt agcccagctt cttgatgtaa tcaaaagcag ttgactggcc gtattggtta
    13261 actgttccag cctgagcagc acccaagaaa gttcctcgaa gatgttcatc tacacccgat
    13321 gtaggtgatt tagtcaaatc acgaatgtgc atttcacaga taactgcctt acatggattt
    13381 tccaagcgcc aagtagcctc cgaaccgtgc ttaacctcga agttttcaac ttgcttttct
    13441 acatggctca gaatagctga acgtttgcca tcagggctgg tcgcgattgt ataaggatca
    13501 cgtgtcagtg tttggtgatg agggaattgg acttgatact gataagtctt acctaccaaa
    13561 tcttcttcaa catccaaact ccagacaccg attgtattgt ccttatgatt ataagagtag
    13621 ctattgcctc ttttcatctc aaaagtcttc caaacgggtg catcattagc agctgattca
    13681 taaacgacaa cttgcacttc tgtcgctgta ggtgaccaga gagaaaaatg agcctgattg
    13741 tcctctacac ggcaacccaa ttctccttgg taaccccaat gatgatcaaa actagcactg
    13801 ttaatggcct tatcaaaggc aaaaggattt tgatttttat agaaaggact ggcaatagca
    13861 ggattttcag agtaataaat cctatcatcg ccttccaaaa tccagacctc tgttaatagg
    13921 ggatagtgat taaaacggat agaatattct ttactagttt gacctgtatg aaccacaaaa
    13981 ttcaagcttt ctataacatg tgaacttggg tgttcaaagc taaataaagc tccaaaataa
    14041 tcttctttgt aggttagcaa atcaattcgt tgatcctgac tttttacaaa ggagcaagtg
    14101 tcatattctc cattcttacg atggtaatga atgcgcatag ggtagttata cattttttat
    14161 ttttcctttt tactttgttt ctatttcact aataaatttt tgtcaatctc gtctcaatta
    14221 acagacatag tcatattctc taaactctgt ttttaaacga tccattacaa actttctagc
    14281 catgcctcat ctctgacctg gataccaagt tcttgtgctt tttgcagttt acttccagcg
    14341 tctgcaccta ccacgacgag gtcggtcttt ttagaaatac tacctgtcac tttggcaccc
    14401 agactttcga gtttactttt agcttctgag cgcttgagtc gttccaattt tcctgtcaat
    14461 accacggtca aacctgacaa ggccgcatcc gctactaccg tctgtccttt atagtccaga
    14521 ttgaccccag tttctttcaa ttctctgagc agaatttcag agccttctgt cgcaaaataa
    14581 gtctgaagac ttttggcaat cacgccacct agactttcaa tactagccac ttcctctgaa
    14641 tctgcctgag acagattttc aattgaatgg aaatattgaa gtaaaagctg actaaccttg
    14701 cttccgacat gacgaattcc caaaccaaat aagagcttct cggcagaatt ttcctttgat
    14761 gcttggatag cctgatacag tttagcagcg gacttttcct taactccctc taaaaggagg
    14821 aaatcctctt cttgcaaacg ataaatatcc gccacatcct tgactaaatt agcagcaaaa
    14881 agcttctcaa caatagatgg accaaggcct gtaatattca tagcatcacg agaagcaaag
    14941 tgaatcaagc cttccatgat ttgagcaggg caacgcggat tgatacaacg tagggccact
    15001 tcatcttcaa agtgcaacaa gtcagagtta caacttggac agtttgtagg gatatctagt
    15061 ttttcttcag aaacccgttt ggactctacc acacgtaaaa cggcagggat gatgtcacca
    15121 gccttatata caatgaccgt atcgtctttt cggatatctt tttcagcaat ataatctaca
    15181 ttgtgcaggg tcgcacggct aacagtcgta ccggcaagtt gtactggtgt tagattagca
    15241 gttggagtta caacaccggt acggccaact gtccagtcaa ctgataagag ttgagcttct
    15301 ttttcttcgg cagggaactt gtaggctact gcccactttg gagccttaac tgtaaaacca
    15361 agttcttctt gacttgctag gtcgttgacc ttgattacca ctccatcaat atcgtaaggc
    15421 agattttccc gttcctgtcc tacttcttgg ataaaattcc agatttcatc tatgttttca
    15481 gccaagattc gcttaggatt gaccacaaaa cctagttgtt ctaggtactt caaacccttt
    15541 tcttggctat cacgagttga agggctggct tcttgataga gaaacgttgc aagattacgc
    15601 ttggcaacta ctgctgtatc caactgacgc agagttcctg ctgccgcatt acgaggatta
    15661 gcaaattcag gctctccatt ttcttggcgc gcttggttaa cttggtcaaa ggaagcgcgt
    15721 ggcatgtaac attccccacg aactgtgata tctagttctt ctggcaaagt caaagggatg
    15781 tccttaacac gcttgaggtt ttctgttata ttttcaccaa ttgaaccatc tccacgtgtt
    15841 accccagcaa ccaaaatccc cttttcataa gtcagcgaga tagataagcc atcgattttc
    15901 agctcacaaa tataggtcgg atgagccact tccttacgaa cacgcgcatc aaaagcatct
    15961 agctcctcac atgaaaaagc atcctgcaaa ctataaagag gatactgatg actgtatttt
    16021 tcaaaaccat ctaaaacctt gccaccaaca cgatgagtcg gactgtctgc tagcacttgc
    16081 tctggataag cagtttctaa ctcgaccaac tcacggtaaa ggcggtcata ctcactgtct
    16141 gaaaccgagg gattatcgct ggtatagtac tcagtcgcat agcgattgag caaagcgact
    16201 aactcattca ttcttttatt cataagacca ttttaccata aaacaagccc tcctcacaaa
    16261 cgagaagggc ggaaaaaaca cttagtttga aattattttt gaaactcaag caaccttata
    16321 tcaatttttc aaaatgagtt cgaacatatc cgagagctaa gaaatataag gctacaactc
    16381 caagtccaat aatcaagaaa gaataaagat ggacacttgg aagaaaggtc attaaacctg
    16441 ttgcttaaat acaatctatc tatatggaat ttgtgactta gttacttcaa tattatgatc
    16501 aacgtattta ataaatggag ctacataatc aatatcatca catttaggtc ctaatgttat
    16561 ttttgaatat tttagattct cactcagata agtaaacagt cttggaactt gcatttctcc
    16621 atatttttca atttggattt cgtcatcatt gcattcttta atcaatctat attcttgctc
    16681 atattcatac ttagattctt taaataaata tctgactata tcaaattctg acaatatcct
    16741 gctttgagaa tctttcaagt agattttttt aagttccttt tttattattt caatttgatc
    16801 taaaataact ttattgtcat cagattctaa ttctttagta taacatactc ggtagaattc
    16861 cactttagaa tcattatcat cagtatctga tttcttctta atcaaacttt ttaaataatt
    16921 tctgtcatag gtaagacaaa tgcctgttgc attatctgca tattgtttcc acattggtaa
    16981 actatcactt aaaccatttt cattcgaaga ttttgacata gaggacaaaa aaatatttgt
    17041 cggttcatag tctagttgca acaatgtatg caaatcaaat ccaacctgtt ttagaaaaac
    17101 agtgccctca gtaggatcat ttaattgcct agcattactc aatctcaatc tagaataatc
    17161 atctgcagta ttttggcttt tcttcaccaa aatgtcaagt gtggagagag aagtataatg
    17221 aactaagtta taatctgcag aaaatttttg aaaattttct aagtcaatcc ttaaaaaatc
    17281 ttttatgcat ttaactgctt ctttaatctt tctgatatgt tcttcatctg aaactaaatt
    17341 atggactaaa ttggttgtgt ttttatcaag atattctata aaacatttta gttgttcttc
    17401 gtttacttgt tgattatttc cactattttt aatcttttca attattttct caagaatagg
    17461 ggtaagataa tctttactcc aagtattaaa aagttttatg tgattgaata atctctcata
    17521 tctctcaaaa gtctcatctt ttataaactt ttcaaatatg gtcgtaactg aatcatcttc
    17581 agaaaaattt ccaatattca aattttcatc aatttctttg agcctttgat ttaaattaat
    17641 aatttccgca ctttttgtta taccatcatt tcttaaaaga gctttcttta ctcctttatt
    17701 ttcttttatt tcttccggta gatctataag tgttaaatct ttatttcttt ccaaaaaatc
    17761 attataatat tctgaacaaa atttgtatag gttttgatag aattctagat tacaagctaa
    17821 agaatatttt tgattcctat caaaatcatc tttagacaat ttaaaaatag attttattcc
    17881 atcagaattc tcttgctgtt ctatttcaga ttcataaatt ctataactgc ctatatatct
    17941 ttcaccacac tccttatcat agcaatagac atgaaaagta atgtaataac catagtcatc
    18001 ccaattatct ttaaaaaaaa caatactttc tgaaaatgtt ttatcagcta cattcttata
    18061 ttctttgtta aagtatctaa attctaccat ctatttgctc cttttacacc tgctctgata
    18121 attcttccca ctcaagcata gcttcttcct gacgatggct gattttgtcc agctcagcct
    18181 gtaattccat gagtttgtcg gcatcgtttg tttccaacat ttgttcagaa atggcttggc
    18241 tttgactttc tagctcttca atttcagctt ctagactttc gatttgtcgc atgagtttgc
    18301 gaacttcttt ttgactttct ttctgggcct gataatcatt gactggactt gcttcctttg
    18361 cttgattgtt agttgaagct tcctcaatct gactcatttc tacttctact ttcttctcaa
    18421 tatagtagtc gtagtctcca aggtagaaag ccgagccatt ctcagacaat tccaaaacat
    18481 gagttgccac acgattgata aagtaacggt cgtgactgac aaaaagcaag gtaccgtcaa
    18541 agtcaatcaa agcattttcc aacacttcct tgctatcaat atccaagtgg ttggtcggct
    18601 catccagaat caaaaagtta ttgttttcca tagacaattt agctaaaagc aaacgagctt
    18661 tttcgccacc agatagcatg ccgactgatt ttttaacatc atctcctgag aaaaggaagg
    18721 ctccaagacg gttgcggatt tcaacttctg gtgtcagttt gaaatcattc cagagttcat
    18781 ccagcaccgt attacttggt gtcagcttgc tttgggtttg gtcatagtaa ccaacctcaa
    18841 cattagcgcc aaagcgcttt tctcccttga taaaaggaat ctggtccaca atagacttga
    18901 taaaggttga cttgccgata ccatttggac caacgatagc gacagcattc atcttacgaa
    18961 gatctaggtt gataggttgt gacaagactt ccccgtcata accaatagca gtattttcaa
    19021 cagtcaaaac aacattgccc gacgtttttt cagactggaa ggtcatgttg gctgctttct
    19081 tgccagcttc aggcttgtcc aaacgctcca ttttttctag ttgtttacgg cgagattgag
    19141 cacgtttggt cgttgaagca cgaactagat tgcgattgac aaagtcttcc agagcagcga
    19201 tttccttctg ttgcttttca tagttttttg cctcagtaac tagcttttgc tccttcaatt
    19261 cgacaaaacg agagtaattc cccacatagc gatccaagga atgcttggtc aaatctagcg
    19321 taattgtcgc aaccttgtcc aagaaataac ggtcgtggct gacgataatg agggcaccgc
    19381 tatagtttac caagtaattc tctagccagg cgatggtttc aatatccaag tggttagttg
    19441 gctcgtccaa gaccaagaga ttgggctttt caaggagcat tttggcaagt gccaaacgag
    19501 tattttgacc accagaaagc tcagcaattt tcatctgcca catagactcg tcaaacttga
    19561 atccattcaa aatcgctcga atatcagctt cataggtaaa gccacctgct tggcgaaaat
    19621 tctcagataa gcggtcataa tctgacatca gtttatccaa atcctcacca gacttttcac
    19681 ccatctccag ctccatctga cgcagttgtc tctccgtccg acgcaaatca ttaaagacat
    19741 gaagcatttc atcgtagatg gtattttcag actcaaaacg gctatcttgg gctaggtaag
    19801 acagagaaat atcttttttc ttattgattt ctccgctagt tggctcctct tctccaacta
    19861 aaatcttcaa aagagtagac ttacctgcac catttttccc aacaagggca atccgatctc
    19921 gttcatcaac ctgcaggttg atattatcga aaagaacctc tcctgcaaaa gaacgttcaa
    19981 ttttattagc ttgtaaaata atcataagag tagtatagca tgtttcccta aggcattcaa
    20041 gataatcgta agtcttttag tacaactttt ataacataaa ataaactaaa ttatgtatat
    20101 tttatattag attccttcac tatcttgttg gattttctaa ccagctaatc ttgtttcaaa
    20161 tagttatcgc acaagtctat tatttaattc ttttcatcat ttacgtacgt atagcagatt
    20221 gaaataagat gagaacaaat cgattgggaa agtaaaatta atttctataa atgttttagc
    20281 aattgtttcg tactatttta gattcagtct actatatata caatattttc ggaacattca
    20341 actttttaac tctatttatt actagatttc ataattaaaa agcctattgt atcaagcttt
    20401 ctagcttggt cagtaggctt tttaaagact gattatttaa cagcgtcttt aagagcttta
    20461 ccagctttga atgctggtac tttagaagct gcaattgtca tttctttacc agtttgtggg
    20521 ttgcgacctt tacgttctgc gcgctcacga acttcaaagt taccaaaacc gatcaattga
    20581 actttttcac cagctgcaag atagtcagct actgctgcaa atacagcttc aactgctgct
    20641 gctgagtctt tcttagtcaa ttctgtagct tctgctactt tagcgatcaa atcttgtttg
    20701 tttgccatgt taatgattcc tccagataat ttctaattaa caaatataat catatcctaa
    20761 aattcctttt aggtcaagtt aaaaacgcta tttcttccaa ttttatttat tttttaggag
    20821 tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt gagtttgaat aatggaaccc
    20881 gtttccaaaa cagaaattgg cttttcaaca taagcttgta agctttcttt catctctttt
    20941 gcccaatcat cactaccaga atatgaaatt ccaatctctg ctacagtacg ttcagaaagc
    21001 gatgttatca actcatctaa ccatttttta aatgttttag ttccacgacc tttaaccatt
    21061 ggctgcaatt catggttttt catttgcatg acaacacgga tattgagaag ggagctcaac
    21121 aagccagtta cacggccaat tcgtccacct ttgacaagat tttccaaagt tgaaacacca
    21181 atataaagct ctgtatggtt tttaacctct tctacatgag ataaaattgc ctccatatct
    21241 ttaccttctt gagctaactt cgcagcctca acaacttgga atttcagggc ttggtcagtg
    21301 aaggaactat caacaacagt cacatctgca gtagataggc tagcaccttg gcgtgctgct
    21361 tctaccgtac ccgaaagagc atgggacata tgaatagcaa gaatctggcc accatctttg
    21421 cataggtctt caaaaatctc agcaaagaca cctacaggtg gctgacttgt tttcggaaga
    21481 ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt ctttcaaatc cgcatcagaa
    21541 taaacaacat tatcaatcat tacagataat ggaacaattg taatatctaa ttgctttact
    21601 agttcaggtt caatagtaac agatgaatcg gttacaatct taatttttgt catagtatca
    21661 atctttctat tttaggattc agattggttt ccttacttct aattatatca aaaaaaagat
    21721 taaaaatcct aatggagtca atcaaatttt ccgtaaaatt tgatataatc aacttataag
    21781 aaaagaggtg tcctatgatt aaaaaaattt accccatttt taccatttta ctaggtgctg
    21841 ctatttatgc ttttggactg acttattttg tagttcccca tcatctcttt gaaggagggg
    21901 cgacaggcat taccctcatc accttttatc tttttaaaat ccctgtttcc ctcatgaacc
    21961 tgctgattaa tattcccctt ttcatcctag cttggaagat ctttggagcc aaatccctct
    22021 attctagttt actaggaacc ttagctttgt ccgcctggtt agcttttttt gagcatattc
    22081 cccttcatat tgatcttcaa ggtgatttac taatcacagc ccttatagcg ggaatcctat
    22141 tgggaattgg ccttggaatt atttttaatg ctggaggtac aactggcgga actgatattc
    22201 tagctcgtat tctcaacaaa tacactcata tatccatagg aaaactgctc tttatcttag
    22261 atttttgtat tctcatgttg attctcctaa tcttcaagga tttgagattg gtttcctaca
    22321 cgcttttgtt tgattttatt gtttctcgtg ttattgattt gattggtgaa ggaggatatg
    22381 ccggcaaagg ctttatgatt atcacaaaac gtcctgacca acttgctaag gcgattaatg
    22441 atgacctcgg aagaggtgtt acttttattt ctggtcaagg ctactatagt aaagaaaatt
    22501 tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt gaaaacgaag gaaatgattc
    22561 atcgaatcga tcctcaagcc tttataacta ttacagaagc ccatgaaatc ctaggagaag
    22621 gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg acctcaaaag ttagactaaa
    22681 tcatctatct tttggattac agacaacctc ttttttattt tatttactca agctcttaag
    22741 accaattccg agttacttct tcatcagcct ttaactgatc cactaattgg tcaactgagt
    22801 caaatttggt catatctcga atgcgatcaa gccaataaac catgacggtt tccccataaa
    22861 tatcttgatt aaaatcaaaa atattgactt caaaacgtgc ttcttctcca tcaaaggtca
    22921 catttttccc gacactagcc atagcacgat acttctgtct ttgaatctca acatcaacaa
    22981 cataaacgcc atctgctggc atataagtac ggtctaaaag cactaaattc gctgtcggat
    23041 aaccaattgt acgaccacga gcattaccat gaactaccat acctcttgat ggaagcggtg
    23101 cccccaaaag ttttcctgct tctttcacat ttccatctaa aatagcttga cggatacgag
    23161 ttgaactaat ctttcctttc tcatcttcta caggtggaac aatgataact tctccatcaa
    23221 agtaatcctt taaatcttct gctgtttttt tgtcagaacc aaatgtataa tcaaaacctg
    23281 caacaataat tttggcattc atagccttga tataagttgc aaagaattct tgtgcagtga
    23341 gactagcgaa ttgactacta aaatcaagga gatataattc ttctacacct tcgcgcttta
    23401 attttctttc acgttcagca gggttcaaaa tatgcaaaaa caaatctgga tgataaggct
    23461 ctaaagcgat ctttggagat tcattaaagg tcataacgac gataggcaat aaatcctttc
    23521 tcgcagcctt gttggcaaca cgaaataatt cttgatgccc cttatgtatg ccatcaaaat
    23581 agccgagaac aacgactgaa tcagatggtg tgccaatatc tttttggttt tttataggaa
    23641 tagtaataat cataaaataa ttatatcata gcgatagcta tttctggaac agaaaatctg
    23701 aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa aagcacttta ttctatcgtt
    23761 gcttaactat gaactttgca atattcttct caaaaacttg taggacatct tcaaaatttt
    23821 gcaaggagtg attagacttg ttcggtaacc ataaagtgtc atactatgct tatgtatgaa
    23881 aaagcaatgc aactaactcc tgagaacttt aaattactaa ttggtgccga aaaggtagaa
    23941 tttagaatcg aggtacacct atgactgtaa aatttacaaa atgagacaac ttgggcaaga
    24001 tgtttgaaga atttcctaaa ctccctgatt tgaagcaagt cactttccct gatgacaaag
    24061 aaaaaagcca aagcaacaaa gaaaaactag atgactgctt tccaacaact cccatctagt
    24121 gtgcttcaga ctgggctatt tttctctcca tctgttagct tggattctca gaccgtttca
    24181 gctaaagaat atcttttccc ttatcagaag gaacggctca agccattcag acaagtgaag
    24241 ggacaacaag ccaatatttg aaaccagata ccagttctta tagtcaattg aaataaaatc
    24301 tgaagaaatc gagtaggaaa ctcatatcaa tttttaacag tgttctattc cagattcata
    24361 ctcaatgaaa attaaagtgc aaactaggaa gttagccgca ggctgtactt gagtacggca
    24421 aggtgaaatt gacgtggttt gaatttgatt ttcgaagagt atcaatccac tatacatatt
    24481 aaaaagtatg ggtatcaaaa taaaaaaaca ggctctccga aaactcggaa agccttattt
    24541 taagctgttt ctagcttcct cacctttaca tttcaaggtt cgggataaaa ggttcaatgg
    24601 acctttttat tttgcgattg ggtaaacaga aacttgtttt ttatcgcgtc ctttacgttc
    24661 aaagcgtact acgccttcaa ctttagcgaa caaagtatcg tctccaccac gaccaacgtt
    24721 tacacctgga tagatgtgtg taccacgttg acggtaaagg attgatccac ctgttacagt
    24781 ttgtccgtca gctgctttag ctccaagacg ttttgcttgt gaatcacgtc cgtttgatgt
    24841 agaacctcca ccttttttgt gggcgaaaag ttgcaagttg ttaagagtca tttttaacat
    24901 aatgttttcc tccgtgttag ttttctgtga taactctggt ttggacgaac tcagaatagt
    24961 tctccgataa gtttgccata cctaagaaaa atgattcaaa gaataactgg gtcatttctc
    25021 tctggtgtga aggaagatct tttggtattt caaccatcag atagccacct tcatcttcgt
    25081 ttaattctag gattggttca tagcctgcaa atttctcaat agaattgata aagttaatgg
    25141 caagcgtaga aaccgatgca cacacgacat ctaagccgta ttcgccactc tcggcgtgtc
    25201 cagtaatttc cgcactcctc agctcgccat cttcggctct ctcaaagact gcttgtatca
    25261 tgtgttctcc ttaaaattaa gcgttaattg cgttgatgac aacttttgta tatggttgac
    25321 ggtgaccttg tttacggtgg ctaccttttt taggtttgta cttgtaagta acaactttct
    25381 tttgttttcc ttgtttttca acagttccaa ctacagtagc tccagcaaca agtggagttc
    25441 cgacaacagt gttttcacca ccaataagaa caacttcgtt aaaagtaact tcttgaccag
    25501 cttcaacgtt caatttttca atgtaaactg cttgaccaac ttcaacttta acttgttttc
    25561 cgccagtttt gataattgcg tatgtgctca ttatgcacct cctatgattt ttatgggttt
    25621 ccccgatttt tttgtgaaga ctcgcctagc atcgtgggac aaaccactta aacttgaata
    25681 atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca tagtcaactc ttcaagtata
    25741 gcatatttcc tattttctta caagtaataa cacctaaaat caagcttttt cttttacttt
    25801 tttctgccac taggcaaaaa gcatactgag ataaaaaatg ctcatcataa taggaacacc
    25861 aagaatggtc ttttcatgat agaaaatcgt caaatagact gaaaagacaa cgccaaggac
    25921 aaaactacta agcaggctaa caaatatgaa cccttcacgc aaaaaaggag tgtgcttagt
    25981 tcggaaataa tctccaaaag ctagcattgt tcgtttgata ttccctgtca taaaggcatt
    26041 attgtaggca atacccgaaa cctcaccaaa agcagttgtc accagtccca tacagaaggc
    26101 caagggcggc actagataga tattatctac agtttgtggc acaaaaccaa taattattga
    26161 taggattgct aaaggaatca aggacagaat agattttttc acaattctca atttttcctt
    26221 ataaatcgtt aataaaaaga ctcccatcat aaacgctatt aaggttaaaa gcttatccct
    26281 aatatccgaa acattatttt gtatgagctc taccgaaaga aagacaacat ttccagcttg
    26341 tccagctaca agggtattcc cgcgaacaat aaaagtgtaa gcatccacat atccagcaca
    26401 aaacgtcaaa aaagtgctaa ccttttagac tgacgtgata tttttcttat aggtaataac
    26461 ctcattttcc ctcccattgt attttctctt agaaatattg taccattttc tttctaaaaa
    26521 atcgtaggct accatttaga ttttactatt agcataaaaa taataataga caactattta
    26581 tccaaaaata gatagaggta acatgtttgc aaacaaagca tacgaacctt tagtaaaatc
    26641 atttccatga aactagaata gagcccctct tagcaaaaat cattatttta atttatttct
    26701 aatcactcct tgacataaat aactctcacc aataaaagac tatgtcttaa aaaaatggta
    26761 taataaaatc aatacttggg cttgatggct atgctactaa taacaattag gagagaaaat
    26821 caggcacttg ttaacaacaa ggattatccc cttgagatga aaggaacttt agaaatctta
    26881 tgatgaacat gcaaaacatg atgcgtcaag cacaaaaact tcaaaaacaa atggaacaaa
    26941 gccaagctga acttgctgct atgcaatttg ttggcaaatc tgctcaagat cttgtccaag
    27001 cgaccttaac tggagataag aaagttatca gcattgattt caatccagct gtcattgacc
    27061 cagaggacct tgaaactctt tctgatatga ccgttcaagc catcaactct gctcttgaac
    27121 aaatcgatga aactaccaag aaaaaactgg gtgctttcgc tgggaaattg ccattctaaa
    27181 aacaaggagc tagaacaata cttatcgata acaaaggcta agaaaggtgc aaaaattata
    27241 aaaaacgcat aatagcaggt gtcaaagcac tcaatattat gcgttttctc atgaaaaatt
    27301 ctcttttcat actcatttaa aatcaaaaag ggaatttatc gttttacgga catgtatatt
    27361 acagatttct ataggtcgtg tatagacagg atatcatcgc atggaagttt tcctttttga
    27421 caccaatcag taatttagtt ctcaggaatt aatggcagtg cttttcgtac atagtttcat
    27481 aactacagta tagcatagta tatcacttta tagttttcac tattgatgaa ctgcttgagc
    27541 tgctgtgata agggttaact tgtaaacatc atctgcatta catccacgag aaaggtcgtt
    27601 aactggtttg ttcaaacctt gcaaaacagg tcctacagcc gcaaaaccac caagacgttc
    27661 agccatcttg taaccaatat ttcctgcctc gatacctggg aagatgaaga catttgcttg
    27721 accagctacc gtacttccag gagctttcag agctgcagtt tcaggaacaa aggctgcatc
    27781 aaattgcaac tcaccatcga tttcaaggtc aggacgcaag tcgtgagcaa ttttagttgc
    27841 ttcaacgacc ttatcaacgc tttcaccaaa ccctgaacct ttagtagaat agctcaacat
    27901 ggcaatttta ggttcgatgc caaacatctt agctgtgatt gctgagttga tggcaatttc
    27961 agccaaggct tctgcatctg gattgatgtt aatggcacag tctccaaata ggtaacgttc
    28021 cgtaccacga accatgagga aggctcctga agtacgagtt acatttggac gagttttgat
    28081 gatttgtaga gctgggcgaa ctgttgaagc tgttgagtga atcgctcctg acaccattcc
    28141 atcaaccaag cccaagtaaa ccaacatcac accaaagtag ttgacatctt caaccaaaac
    28201 cttgcgtaca tcttcttcag tcattttgcc cttgcgacgc tccaccaagg cagaaaccat
    28261 ttcttcaaat tgaggataat gttgagggtc gatgacctca taaccatcca tgattccttc
    28321 aatttcaaga taaattttaa ttttttcagg atttccaagc aaaacaggaa tcacttctgt
    28381 ttcttttact aagcgttttg ttgcttgaag aatacgaggc tcttcccctt cagggagaac
    28441 gatacgagca tttttaccaa caaggttggc tttgagactt tcaaaaactt ccatgagttt
    28501 tctcctttaa gataataatt atactctgag acagttttta tagagtatta gttgataact
    28561 gggtaataag gttactgaaa tcatccggca agggactttc taactgcaag tcttgctcta
    28621 aaaatggatg ataaaaggat aggtaatggc aatgcagagc ctgacgttga ataccatctt
    28681 ccagactacc accatacaaa tcatctccca gcaaaggaaa accgatatga gaaaaatgga
    28741 ctcggatttg atgggttcga ccagtgtgca ggtgaatata gaccaagtga atatttccat
    28801 aagaagctac aatcttgtat gaagtatggg catactttcc gcctttagcc actcgtctgg
    28861 taataatgga atcttcatca cgcgcaatcg gagcaataat ttccccttct ggctccaaat
    28921 gtccatctcc cttaaccaaa gcaaagtagc gtttctcgat agatttcttc tgcaactgct
    28981 tgtctaatcg tgcatgggca taaccgtgct tggcaaagag catcaagcca gaagtatccc
    29041 tatctagtct ggtaacaatg tgaacctgct gattttcata attttgcttg acatagtaac
    29101 ccttgataaa attggcaatg gtattagagt gattgacact aggaatagaa gccactccat
    29161 agggtttatt caagactaga aagtggtcat cctcatagag aatatctaat ggaagctcaa
    29221 tagcctccaa ggtttcaaag cctttctcag cgggaatgtc aatggtaacg tagtctccaa
    29281 cgtccaatag atacgttgca ttttgcggtt gattattgac cagaatagct ccacctcgaa
    29341 acttaatctt ggccagcaat cccttagaaa cctcgtgctt ttttaagaag gtcttaacct
    29401 tgacatgttc atctgcgata aattcaaacc tcattcatcc acctcgccga taaaggcgtc
    29461 cttaacacgg ttccagaaac tggtatggct aggagtcgcg acaaagtgaa tcttatgatg
    29521 gtcgatttga tactcaatac gctcaatatt acggaaagaa taaacgctat tgtcaaccga
    29581 aatagtatga taatcgtttc ttgttggaat aagttcaatc ttatccttct taggcacaat
    29641 aatggaagag cccagtgttc gatagacacg attattaagg ctggcaattt ccgttaattg
    29701 caaagcttca atggtagggt gtaaaacagc accgccaaga gacttgttat aggcagtact
    29761 accagtcggt gtcgaaactg ttagcccgtc tccacgaaaa cgttcaaagg gaacaccatt
    29821 tattacaata tctgccacca tggttcgatc agacctgcgg atgctggctt cgttgagtgc
    29881 tctgaaaatc ttaacttcac cattttcaag aaagaccttc acattcagaa cagggtaaga
    29941 aacccttgcc ccagtatcta gctgcaaatt agtcactagc ttgtccaact caaaatcacg
    30001 ataatctgta tagaagccca aatgtccagt atgaagaccg ataaagcgga ccttgtcaag
    30061 ctgattttcg tacttatgaa aggccgacaa gagcatacca tccccgccaa tggaaatgac
    30121 aatatccgga ttggtatcat tgagtataaa ctgatttctc ttcaaacgat ctcgcaattc
    30181 atacaaaacc ctttgactct gcggttttct attggctatc agatcaattc gtttacctgt
    30241 attcttcatc tgtatcgtca ctgtttccta caccgtcatt taattttcta ctcaaaggat
    30301 caaaaagtgc ctgggcttct tggatatcat cacgaatttc acccatttct tcatccaact
    30361 gatgggcgat tctagctgta atttccagtc gcttcttaat ctcatctggg aaatcccctt
    30421 ggtacttgta gttgagagaa tgttctatcg ttgcccagaa attcatggcc aaagtacgaa
    30481 tttgaatttc tgccaaaata gtcttagctc cattgatggt atcaaccgta tattctacta
    30541 ccacatgata ggaacgatag cctgatgctt ttctatgagt aatgtaatct cgctcctgta
    30601 tgattcgcat atcctgacgc ttgtgcaaaa tatccactac ttccttgacg tcatctacaa
    30661 actgaaccat cacacgtaag ccagcaatat cctgcaaatc gtgttccaag gtcgcataag
    30721 taatgccacg acgagccatt ttttctttga tgctctcaat tggcttgact cgaccggtca
    30781 caaactcaat tggagaatgc ttattttgct tacgatattg cttacgaata ccacgaagtt
    30841 taatctttaa ctcaccaaca gcttgaatgt aaggatctag aaattcttcc cattctaagg
    30901 tcatatattc tcccttgttc tcatattatc cgtcaaaaat atctttgatt ttttctccag
    30961 attcatatac aataaataca atgcttatta taccataaat ccgctttcaa cgataaaaaa
    31021 aggtaagaaa aatgaaacat ttagaaattg aattgaaaac actattgaaa aaagatgaat
    31081 acaatcgtct aaaagaccag ttcacaggtg tcactcctgt tcttcaaaca aattactaca
    31141 tcgacacgcc tgattttgaa ctgcgagaaa agaaagtcgc tatgcgcatt cgaacctttg
    31201 aagactgggc tgagttgaca ctcaaagtcc cgcagagtgt tggaaacatg gaatacaacc
    31261 aaaaattgca actaaaagat gctgagaact atctgagtaa ggaagaactt cctcaagggc
    31321 tagtactgga tgaattggcg aaacatggta tccaaagtaa gaactggcag gttcttggtt
    31381 gtctaacaac gcttcgctat gaaatgaaaa cagctattgg tctcatggca ctggatcaga
    31441 gccgatactt tgatataaca gattacgaat tggagcttga agtggaaaat catgagcaag
    31501 gcaaacagga ttttcgacaa tttttagaga aaaatcagat ttcctatcaa aaagctcctt
    31561 caaaattggt tcgatttgtc aaaagtatga aaaatagctg aaataatctc cattttttgg
    31621 taaaatagaa aagataaaaa tacacaaatc ccagttcata tatgtaaggt aaatataact
    31681 aggatttata aagtttacag aggacggtct ataatgtcag atagaaaaaa catgaaactt
    31741 ttcgcactca actctaacca agagattgca caaaaaattg cccaagctgt tggtgtccca
    31801 cttggaaaac tatcatcacg tcaattttca gacggagaaa tccaagtaaa tatcgaagaa
    31861 agtgtccgtg gttatgatgt ttacatcatc caatcaacaa gtttccctgt caacaaccac
    31921 ctaatggaat tgttaatcat ggtcgatgct tgtgtgcgtg caagtgccca cagtatcaac
    31981 gttgtccttc catattttgg ctatgcacgt caagaccgca ttgcttgtcc tcgtgagcca
    32041 cttacagcaa aactagttgc caatatgctg gttaaggctg gagttgatcg tatcctgact
    32101 cttgatttgc atgccgttca ggttcaaggt ttctttgata ttccagtgga taatcttttc
    32161 actgttcccc tattcgcaaa acattactgc gataaaggat tgcttggttc agatgttgtt
    32221 gtcgttagcc ctaaaaattc aggtgtcaaa cgtgcgcgta gcctggctga atatcttgat
    32281 gctcctatcg ccattatcga ctaccctcaa gacgatgcaa ctcgtaacga aggttatatt
    32341 attggtgatg ttgaaggtaa gaaagctatc ttgattgatg atattttaaa tacaggacgt
    32401 accttctctg aagcttctaa aatcgttgaa cgtgaaggag ctacagaaat ttatgctgtt
    32461 tctagccacg gtctcttcgt cgagggagct gctgagcttc ttgacaatac taatattaaa
    32521 gaaattcttg tgactgattc agtagcaaca aaagaaaaaa ctcctaaaaa tgtatgctac
    32581 atcactgcta gtgagttaat tggtgatgct atcgtccgta ttcacgaaag aaaaccagtc
    32641 agcccactct ttgcctacaa taaaaagaaa taaggtgatt ctttgattta tttggacaat
    32701 gctgcaacga ctcctatgtc agcagttgct atttcagcta tgaccaaggt tatgcaagaa
    32761 acccacggaa atccttctag tattcatggt catggtcgtc aagctggcaa actcttgcga
    32821 gaagcccgtc aggaactagc ccagttacta aggacaaaac ctcaacatat ctttttcact
    32881 tctggtggga ctgaaggcaa taatactacc atcattggct actgtcttcg tcaccaagaa
    32941 caaggaaaac atatcatcac aactgccatc gagcaccatg ctgtccttga aacaattgat
    33001 tacttggttc aacactttgg gtttgaagca accattatcc agccagaaaa tcaagaaatc
    33061 acagcccagc aaattcaaaa ggctttacgt gacgatacga ttttggtttc taccatgttt
    33121 gtcaataatg agacaggaaa cctactgccc atcgctgaaa ttggccaaat actcaagcaa
    33181 caccctgctg cctatcatgt tgatgcagtt caggctattg gtaaaatccc aattcattca
    33241 gaagaattgg gcattgattt tctcactgct tctgcccaca aattccatgg tcctaaggga
    33301 atcggttttc tctacgcatc tagcatggac tttgattcct atctacatgg cggagaccag
    33361 gaacagaaaa aacgtgcagg aactgaaaat ctgcctgcca ttgtaggcat ggttgcagcc
    33421 ctaaaagaag acctagaaaa acaagaagaa cattttcaac atgtacaaaa tctagaaact
    33481 gcctttctgg cagagctgga gggcattcag tattacctga atagaggaaa acatcatctc
    33541 ccttatgttc tcaatattgg atttcctggt cagaaaaatg acctcttact ccttcggcta
    33601 gatttagctg gaatttcaat ctctactggc tcagcctgta ctgcaggcgt tgtccaatcc
    33661 agccatgttc ttgaagccat gtatggcgca aattcagaac gcttgaagga atcccttcgc
    33721 atcagtttgt cgccacaaaa taccgttgaa gacctacaaa ccctcgcaaa aaccttaaaa
    33781 gaaattatcg gaggttagcc atatggcatt tgaaaaaatc attcagttaa aaaattgtcg
    33841 ttacgattac actcttagcc cttctgttaa aaaattcacc ctcaaagata acaccttttt
    33901 tgaaactaag gttggtaact atgaactgac tcgccttttg gaaaaagtgc caaacagcgg
    33961 tgaaggcttc caactcaaaa tcatcattaa caaggaactt acaggggcta aaatcaatat
    34021 cactgacaag tttggccttc gtctagttga tattttcaaa tcagaagacc accatattca
    34081 tcaggaaaaa ttctacttcc tcatggatag cttggtagaa cgtggtgtct ttacaaaatc
    34141 ggaaagatag agccaaaatg tttgaactga cctataagga ctgctatcat gtagagcgta
    34201 ctctcaagta tgaggatcat gaggctctca tgttgacttt gtcaggatgt gtaaccttac
    34261 cagatacact ttatgtgact tctctgactt ttaggggcca aaaagtttat cagggtctgg
    34321 tcggagacct ctaccgtttt ctatctcatg cagatttttt acatcaaaac taagattttt
    34381 cttagttttt tcttattttt gttgattttt tcacaatgtt tctataaaat agaagaatag
    34441 aaaggttgtg attttgtgaa agataaacag tttgctattc caaaagctac agcaaaaaga
    34501 ctctctctct actatcgaat ttttaagaga tttcatgcag aaaagattga acgtgccaac
    34561 tctaagcaaa ttgcagaggc tatcggtatt gattcagcga ccgtacgtcg tgatttttcc
    34621 tattttggtg aacttggtcg tcgtggtttt ggctatgatg tcaaaaaact aatgacattt
    34681 tttgccgatt tgctcaatga caactctatt accaatgtca tgctggttgg tattggaaat
    34741 atgggccatg cccttctcca ctaccgcttc cacgaacgta acaagatgaa gattatcatg
    34801 gcctttgacc tagacgacca tcctgaagtc ggtacccaaa ctcctgacgg gattcccatt
    34861 tacgggattt ctcagatcaa ggataaaatc aaggatgctg atgtgaagac tgctatccta
    34921 actgttccca gcgtcaagtc acaagaggtt gctaatctct tggttgatgc tggtgtgaaa
    34981 ggaattctca gtttttcacc agtccatctg catttaccaa aagacgtggt cgttcagtat
    35041 gtcgatttga caagtgaact ccaaaccctc ctctacttca tgcgaaaaga ggattagaaa
    35101 gcgaaaatca ttttatgaaa aaaccagtta ttgggattac aggaaacgaa aaaactcatc
    35161 cagatgatga catcatgatg agctacgcag caaaaggctt tgttgaaggc gttaaagatg
    35221 ctggagggat tcccatcatc ctaccgattg gtgatcaaga aatggcctgc cactatatca
    35281 gtttgattga caagctcatc ttgacaggtg ggcaaaatgt cgatccaaaa ttctatggcg
    35341 aacccaaaac cattgatagc gatgattatc accttcaaag agatatcttc gaactggccc
    35401 tcatcaagga agctattaaa cagaaaaagc ccattttctc tgtctgccgt ggtacccaac
    35461 tctttaacgt tgccatgggt ggaactttgt accaagatat cgaagaccat tggcaggatt
    35521 cttctgttga gtacacaact caacgcttgg taacagaacc agataccgtt ctccaagaaa
    35581 tctatggaga aatctctcat atcaactcct tccaccacca gagcatcaag gacttagcac
    35641 caaatttaaa agttgtggct catgatccta aagatggtat tattgaggct gtcatgagta
    35701 cggatgatgt cgcctttctc ggtgtccaat ggcatccaga acttcttttt gaaaatcgtc
    35761 ccaaagataa gaaactcttt gactatgtcg ttaatgaact ttagattaaa tctgtctttt
    35821 cacgataact aaagtaatta gaatgagaga caatcaaatg gtccaagaga acaatcccca
    35881 tcagttcgca ggcttcttta acaagtttag tgacatgatc atcattttgg ctaggcgcta
    35941 ccgctcctga aggatgattg tggaccaaga taagagaagt cgccatatgc ttgattgcat
    36001 agtgaagaat ctctcgcggt tcagcgatac tacgagttac agaaccgata aaaatggtct
    36061 gctgatggat gatttgattt tgagtattga gatagagtgc caccaggtgc tcttgttttt
    36121 tatcccctaa ttcctgctgc atcttcttgg ccaacttttg actgctgaga atactttcca
    36181 tttcaagagt ctcgtgtttg tgaatacgat gccccagttc aatcatagct tgtaattcta
    36241 tggccttaac acgcccaata ccagacaaac tctgcaattc ctgcagggtc atttttttca
    36301 aatccgttag gcttgaaaga ttgttcaaga ctttttgggc aatttcaaaa acgctagctt
    36361 gacgtgttcc tgtcctgagt aaaatagcta gcaactcttg gttactaagc gcttcaactc
    36421 cttccttggc cagcctttct cttggtaata gtgaatcttc ttggaatgaa atactgtaca
    36481 taaaaatcct cctcacttta ttattcgtga gaaggatgga aaattagatt tttttctcaa
    36541 ttggagccac actggctaaa agttttttca aacctacttc tgggaaattg attttcaatt
    36601 cctgcctagc accgctacct gaaacttcca gaacggttcc ctctccccat ttcttgtgga
    36661 gagcaatatc accaatggac caatttgcct cgctagatgc tggttttgcg ccagctgtaa
    36721 attgaccaaa tggaagcccg cttgactgga ttgattttgg ggcagcaccg cgtttacggt
    36781 cttgaagagc ctgagccaaa ctcatacctt gaccaaagga aatactacca ctgctatatg
    36841 atgccttaaa gcttgtattt gcaggacgag ccagaccttg atactcaagc aagtctgaac
    36901 tgatttcgtt aataaaacga gtcggacggt tataattggt acgaccaaaa agcaagcgtg
    36961 agttggcatt ggtcagatag agaattttct ctgcacgcgt gatacctaca taggctagac
    37021 ggcgctcttc ttctaattca tctggatctt cagtcgcacg actaagtgga aagacatttt
    37081 cttccatccc aatcaaaaag acaactggaa attcgagacc tttggcagca tgcagggtca
    37141 tcaaggtcac ttctgatgtc tcctgactac ctgaatctgt gtcggcaatc aaagccaagt
    37201 catttaagaa acgactcagt ttgtccagac cagtttcctc ttctgtcaca tccgtggtgt
    37261 catcaaagtt cttcgtaaca gaaagaaact cttcgatatt ttcaacccgt gccttgcttt
    37321 ctagagtcgc ttgggcgtta agaatatcga cataacctgt tttttctagg acggactcaa
    37381 ccaactctgt aatgcttaag tggtctagct gctcccgcaa atcaagcatc atattggcaa
    37441 aatcccagat agattgggct gccttaccct tgataccaga caacataata ttagcagaag
    37501 catccagcat agacatattt tgcaaatttg caaaatcacg gattttctca actgtacctg
    37561 gaccaattcc acgtttaggc tcgttgataa tacgctcaaa actaatattg tcactcaaat
    37621 tagcaataag gttgagataa gcaataatat cgcgaatttc cttacggctg tagaatttgg
    37681 ttccgccaac catggtataa ggaatgttag acttgagcag ggcttcctca attgtacggg
    37741 actgggcatt agtccgatag agaactgcaa aatccttatg aaggaagttt tgactgcgac
    37801 taagttcatc gatggttctg gctacaaata cagcctcatc cagctcatca tcggcacgat
    37861 agtaaacgat ttgctcccca tcagcgtttt gagtccagag atttttagga cggcgatttt
    37921 tattgttttt aataacctcg ttggccgctt ggagaatggt tttggttgag cggtaatttt
    37981 cctccaacaa aacaaccttg gctttggggt aatccttttc aaagtccaag atattctgca
    38041 tatcagcacc acgccaaccg tagatagatt ggtccgcatc cccaaccaca cagatatttt
    38101 taaaacggga agccaagagt ttgaccaatt ggtactgagc gtggttggta tcttggtact
    38161 catcaacgtg gatgtattgg aatttttgct ggtagtaggt caaaacatca ggattttgat
    38221 caaagagacg caaggtcagc ataatcaaat catcaaagtc aacggattca gactgacgaa
    38281 gttctttttg ataggctgta taacactggg ccacaatttg cgtatacata tcgccagctt
    38341 gggcagcata agcaacatca tcaatcaaat cattcttagc attggaaatg gtccccaaaa
    38401 tagttcgttc attccatttt ttaggatcca agttcaactg tttgagaata cgtttcatga
    38461 gcgttcgctg ttcaccagga tccacaattg taaaattacg attgtagcca atatggtccg
    38521 catcgcgacg caaaatacgc acacacatgg agtggaaggt cgcaatcaga cagtcctgag
    38581 tagctggatt gaggctataa gcacgctctt tcatctcacg cgcagccttg ttggtaaagg
    38641 taatggccaa gatattccaa gaattgacca gcttttcatc aatcaaataa gcgatacggt
    38701 gggttaaaac acgagtcttt ccagaaccag cccctgccat gattagcaag ggaccttctg
    38761 tcgtttgcac cgcctcagcc tgacggtcat tcattccatt taataatgcg ttcatcttct
    38821 cttccttact cttgaagtca atatttctat tatatcagaa atcagggaaa atagaaactt
    38881 tacggggagt agcaaaatat agtggattga aactagaata ctacactgtg ggtttctaaa
    38941 acactcgact tttcacccaa ttttttcata aactagctca atacctcaat agacttcctg
    39001 caaaactaga atcctagttc atgattgata atacca
//
LOCUS       DAVDWM010000014        36978 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVDWM010000014 DAVDWM010000000
VERSION     DAVDWM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.014"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            690..1058
                     /locus_tag="VUW49_000911"
     CDS             690..1058
                     /locus_tag="VUW49_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HET7980852.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVDL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALTVTGFSLG
                     GAIAYGVDWADGSLDGYIFA"
     gene            complement(1458..2321)
                     /locus_tag="VUW49_000912"
     CDS             complement(1458..2321)
                     /locus_tag="VUW49_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7980853.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            2787..3125
                     /locus_tag="VUW49_000913"
     CDS             2787..3125
                     /locus_tag="VUW49_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET7980854.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            3103..3597
                     /locus_tag="VUW49_000914"
     CDS             3103..3597
                     /locus_tag="VUW49_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980855.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKSACQGICAALAMSPGT
                     FIALTGAAVLTKKLINYIKVGGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFK
                     ASKVMKRRIFSLFVLDLMVYIFLGYVLVIQKDVYLFSILIIFSNFSVPFIREKEYELF
                     KNKK"
     gene            4166..5224
                     /locus_tag="VUW49_000915"
     CDS             4166..5224
                     /locus_tag="VUW49_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980856.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPFSVFTFMFSYILLFVQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            5539..6516
                     /locus_tag="VUW49_000916"
     CDS             5539..6516
                     /locus_tag="VUW49_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980857.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINNPISLYGMLDVIKLNLYLRNYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYIGIKHVSIRR"
     gene            6893..7267
                     /locus_tag="VUW49_000917"
     CDS             6893..7267
                     /locus_tag="VUW49_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET7980858.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINRFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            7475..8377
                     /locus_tag="VUW49_000918"
     CDS             7475..8377
                     /locus_tag="VUW49_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980859.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEMSISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            8433..8786
                     /locus_tag="VUW49_000919"
     CDS             8433..8786
                     /locus_tag="VUW49_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7980860.1"
                     /translation="MELVLPNNYVALEQEEMIYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            8783..9511
                     /locus_tag="VUW49_000920"
     CDS             8783..9511
                     /locus_tag="VUW49_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980861.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            10644..10883
                     /locus_tag="VUW49_000921"
     CDS             10644..10883
                     /locus_tag="VUW49_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980862.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            10868..11146
                     /locus_tag="VUW49_000922"
     CDS             10868..11146
                     /locus_tag="VUW49_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980863.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVASDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYVKVRKTDVKEMKWL"
     gene            11432..13672
                     /gene="pspA"
                     /locus_tag="VUW49_000923"
     CDS             11432..13672
                     /gene="pspA"
                     /locus_tag="VUW49_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HET7980864.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPVTVKAEVASTASASSTVE
                     QAKSKVDEAQKNYNEAQKNYNEAQKKVEEKANAVKKLDEEVQKANEAVQRAYLEQRKA
                     QEEKNKHPRKKEYIKKLEEANKKIDEEKDKQKSKQTAYTQKMSESIPDVNPLLTRLKT
                     EKDNLEAAKRQLKEAKAAEKTEKEAKVKKVETELAQAKEKVKKQAEEDRRNYPTNTYK
                     TLELEIAESDVKVKEAELELVKEEANESRNEEKIKQAKAKVESKQAEATRLEKIKTDR
                     KKAEEEAKVKLEEAEKKATEAKQKLDAERAKEVAPQAKIAELEYEVQRLEKALEEIDE
                     SESEDYAKEGFRAPLQSKLDAKKAKLSKLEELSDKIDELDAEIAKLEDQLKAAEENNN
                     VEDYFKEGLEKTIAAKKAELEKTEADLKKAVNEPEKPAEEPSQPEKPAEEPSQPEKPA
                     EEAPAPEQPTEPTQPEKPAEQPQPAPAPQPEKTDDQQAEEDYARKSEEAYNRLTQQQP
                     PKTEKPAPAPQPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGA
                     MATEWLQYNGSWYYLNASGAMATGWAKVNGSWYYLNANGAMATGWLQYNDSWYYLNAS
                     GAMATGWAKVNGSWYYLNANGAMATGWLQNNGSWYYLNANGAMATGWAKVNGSWYYLN
                     ANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYTVN
                     ENGEWV"
     gene            14073..15194
                     /gene="mnmA"
                     /locus_tag="VUW49_000924"
     CDS             14073..15194
                     /gene="mnmA"
                     /locus_tag="VUW49_000924"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7980865.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVIVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            15335..15790
                     /locus_tag="VUW49_000925"
     CDS             15335..15790
                     /locus_tag="VUW49_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7980866.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQYGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            15800..17713
                     /gene="mnmG"
                     /locus_tag="VUW49_000926"
     CDS             15800..17713
                     /gene="mnmG"
                     /locus_tag="VUW49_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7980867.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(17920..18012)
                     /locus_tag="VUW49_000927"
                     /pseudo
     CDS             complement(17920..18012)
                     /locus_tag="VUW49_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(18066..19745)
                     /locus_tag="VUW49_000928"
     CDS             complement(18066..19745)
                     /locus_tag="VUW49_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET7980868.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(19747..19980)
                     /locus_tag="VUW49_000929"
     CDS             complement(19747..19980)
                     /locus_tag="VUW49_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET7980869.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            20260..20475
                     /locus_tag="VUW49_000930"
     CDS             20260..20475
                     /locus_tag="VUW49_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980870.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(20798..20950)
                     /gene="cibB"
                     /locus_tag="VUW49_000931"
     CDS             complement(20798..20950)
                     /gene="cibB"
                     /locus_tag="VUW49_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET7980871.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(20952..21137)
                     /gene="cibA"
                     /locus_tag="VUW49_000932"
     CDS             complement(20952..21137)
                     /gene="cibA"
                     /locus_tag="VUW49_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET7980872.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            21315..21998
                     /gene="tsaB"
                     /locus_tag="VUW49_000933"
     CDS             21315..21998
                     /gene="tsaB"
                     /locus_tag="VUW49_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7980873.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            21995..22432
                     /gene="rimI"
                     /locus_tag="VUW49_000934"
     CDS             21995..22432
                     /gene="rimI"
                     /locus_tag="VUW49_000934"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7980874.1"
                     /translation="MIEIKRIQQQPALAQDIYAVMAAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLTVQETLFEAEVLQIAVKGACQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            22422..23432
                     /gene="tsaD"
                     /locus_tag="VUW49_000935"
     CDS             22422..23432
                     /gene="tsaD"
                     /locus_tag="VUW49_000935"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7980875.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(23472..24778)
                     /locus_tag="VUW49_000936"
                     /pseudo
     CDS             complement(23472..24778)
                     /locus_tag="VUW49_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(25054..25405)
                     /locus_tag="VUW49_000937"
                     /pseudo
     CDS             complement(25054..25405)
                     /locus_tag="VUW49_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(25512..25805)
                     /locus_tag="VUW49_000938"
                     /pseudo
     CDS             complement(25512..25805)
                     /locus_tag="VUW49_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            26048..27222
                     /locus_tag="VUW49_000939"
                     /pseudo
     CDS             26048..27222
                     /locus_tag="VUW49_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            27215..28168
                     /locus_tag="VUW49_000940"
     CDS             27215..28168
                     /locus_tag="VUW49_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7980876.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLFLKYMLLYHFGFFYCIYDNYVQLKFRK"
     gene            28178..29794
                     /locus_tag="VUW49_000941"
     CDS             28178..29794
                     /locus_tag="VUW49_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980877.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            30055..30483
                     /locus_tag="VUW49_000942"
     CDS             30055..30483
                     /locus_tag="VUW49_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980878.1"
                     /translation="MSEIVEMSKKISDLNDKKEFHKKNCELRFQDSVLDNINSVPGTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIE
                     RILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            30495..31245
                     /locus_tag="VUW49_000943"
                     /pseudo
     CDS             30495..31245
                     /locus_tag="VUW49_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            31258..32429
                     /locus_tag="VUW49_000944"
                     /pseudo
     CDS             31258..32429
                     /locus_tag="VUW49_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            32825..33688
                     /locus_tag="VUW49_000945"
     CDS             32825..33688
                     /locus_tag="VUW49_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET7980879.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLIALFE
                     LRFQQYKELID"
     gene            33974..34207
                     /locus_tag="VUW49_000946"
     CDS             33974..34207
                     /locus_tag="VUW49_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980880.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            34229..34906
                     /locus_tag="VUW49_000947"
     CDS             34229..34906
                     /locus_tag="VUW49_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980881.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            34918..35583
                     /locus_tag="VUW49_000948"
     CDS             34918..35583
                     /locus_tag="VUW49_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7980882.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            35655..36881
                     /locus_tag="VUW49_000949"
     CDS             35655..36881
                     /locus_tag="VUW49_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7980883.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT        11732 a         5840 c         7567 g        11839 t
ORIGIN      
        1 caaatttggc ttgtaatttt tttataaaat tcacttcaga tacttgacag ttattatatt
       61 ttcgcttatg cttattatat agataaaagg acgggatagg agtttaaaaa tttgaaagcg
      121 ctatccataa tcataacgca taattttctc cttttcgtga aaaaagttaa cgtaattttg
      181 aaatgagctt aataagaggt actatgtagg tagaaaataa agataggaga aaaagtatga
      241 ataaaaaaat attttgtatt ctagtatgta ttatatcatt gattagtcta gctataatct
      301 ttccatgggg ctggtcgata taatgtaagg gagcttgagt tgttagtgga ggataaaatg
      361 ttaagagtaa tagtagttat tcaatcttta gtaataatac tgcttgctgt tgctttatta
      421 ttatctcgga aaaactttga ttcgttaaat gttgttctta taatgatgat cgtatcacaa
      481 ttgttgttat tagtatattt gataaaatca gggaatcagt gattagctgt tatttgaatt
      541 aagtaaactg ttaagcttca agcagtaact ggttttaaat atttaaaata agataaagga
      601 aggaagtcta atttaggtaa ctaaacaaag cctgcgatca cttgttagga aaccatccta
      661 accatgctta ataaaaaagg agaaaataaa tggaactcgt attaccaaat aattatgttg
      721 ctcttgagca agaagagatg atgtatcttg atgggggtgg tgttggtcgt aactggtgga
      781 atagtagagg tagttttgca acagttctgg atgtagattt ggccatctat agtggtggtg
      841 caacaattta ttctgcttat gcgataaaaa aagctatctc agctaataga ggggctatta
      901 cgagaacatt acgtagttta ataattaaac atgtaggtag tgcagctggc catttagtca
      961 atactgcact aactgttact ggattttcac taggtggagc aatcgcatat ggggttgatt
     1021 gggctgacgg tagcttagat ggttatattt ttgcttaaaa tgaaacattt ttttgcagga
     1081 attggtgaaa taaactttgt aagttattta ttgtatatat gtgtaggaat agcccctttt
     1141 tttcacgtat atataatagg ttctgaaatg aattttgtga aaatagtgtt atctattttg
     1201 ggaattatat ttgtttgtat tttaactatt gctcgaattt ataggacttt tttctataaa
     1261 gaatagtttg gacttgaaat gtatttaaaa acaagaggtt ggtagattat cagcctcttt
     1321 cttgtcgttg aaaaagtgag atatattgac ttttgaaaaa aatgtcataa ttgagttggg
     1381 ataaacgtaa aagtcatact ttctcgttaa atcgttcggc gattctatga aaaggtcgag
     1441 gagctccctc ggctttttta tctttccttc gcttggtaat agatctctac ttcttttttg
     1501 agatgagaca gcatagaagt ttcctcggtc agctctagga gtgagaaacc tttttggata
     1561 agtttagaat cgtctgtaca aatcccaatg cggacatagc agatagccag cctatcgtag
     1621 cttttcttat tcttggcgtc ctccagtaaa gtgtagcaga tttgcttaca catgttttta
     1681 tcttgattgt atagatagag tgtggaaagg ttgagcagga ttgctattcg caagtcatat
     1741 aaatgttgat agtttttata gacttctaag cgttgcaaga tttttccagt gatgagatgg
     1801 aggtattcaa tgggaaaact gaaaagaatg gtattgagga tttttagatc actctcatac
     1861 catgtatctt gtttttcaat ttttctccaa agttttttga caacctgttc tgcgtgatct
     1921 gatagttccc ctgccccgtg ttgacggata taaatgacaa cttcaagcat atctctgatt
     1981 tcttctatag ggagatcgtg gtgggtcttg aggtagtctt ggcatttttg aaataaattg
     2041 acaagatcgc tggtcccgat aatggaacgc atattgagat aggtttgcat aatctctgtg
     2101 cgttgacttg gttgataaag ttgacagata tattcaaact cttcaaaact catattgatt
     2161 tgacggagaa gaaattccat attttcatat tttggggttg tcttgccact ttcgattttt
     2221 gataagctgg ttcttgagag aacaccccca cagacctctt cttgggtcag cccctttgac
     2281 tcacgtattt ctttatagac ctttccgaaa tcataacgca tgattttctc ctttctgtga
     2341 aaaaagttaa caaataataa aatcttgtta aaaatattgt atcatagcag tgtaaaaagt
     2401 aaaaaagaaa gtaaaaataa atttgtgaaa aaagttaacg taattttaaa atgagcttaa
     2461 taagaggtat gatgtaggta gaaaataaag ataggagaaa aagtatgaat aaaaaaatat
     2521 tttgtattct agtatgtatg atatcattga ttagtctggc cataatcttt ccatggggct
     2581 ggtcgatata atgtaaggga gcttgagttg ttaaaggagg ctcagtcctt ggacaaaaat
     2641 ctgctgcact acaatgcttg gaaagatatc aagaacaaag aatgatacgg ttgaaaataa
     2701 actaattcaa gaactactgg gcagtgattg aggtttgggt gacatcaaag aaaagttaga
     2761 ttaattctaa agaattgagt atttctatgt taaaaaaata tccttgtacg atgcaacatg
     2821 accagtcaga ttgcgctgcg gcagttgttt caacagttct tttatcttac aaaaaggaac
     2881 tatcaatcat gaagattcgg gaaattattg gtacagacat gtatggaacg actgtcagtg
     2941 gtattgtttc aggtctgaat aagttgaatt ttacagtaaa agctgttcga gtagcactgg
     3001 aagatttgac tccaaaatta acatttcctg cgattcttca agttaagaat gatttaggtc
     3061 aaaatcattt tgtggtatta catagtataa aggagaaaat aaatggaact cgtattacca
     3121 aataattatg ttgtgattga tgaagaagag atgatgtacc ttgatggggg ggcttattta
     3181 agcaagagtg cttgtcaagg aatttgcgca gctttagcta tgagcccagg aacttttata
     3241 gcattgactg gagctgcagt tttaacaaaa aaactaataa actatattaa ggttggaggc
     3301 cttggaggtt ggcttattgg tgcagcagca ggtgtattgg ctcttatttt tttgataaaa
     3361 atttgtgtag atactacgat aaaggttcta ttaaacagaa tctttaaggc aagtaaggta
     3421 atgaaaagga ggatattttc tctttttgta ctagatttga tggtctatat atttttaggt
     3481 tatgttttag tgatacaaaa agacgtatat ctgttttcaa ttctgataat tttttctaat
     3541 ttttcagttc cctttatcag agaaaaagag tatgaattat ttaaaaataa gaaatgagat
     3601 agatatggta gctttattaa aactgagaat aaagtttggg agatattttt atttatttta
     3661 ggtgtgttgt taattgtaat ttcattacta agtcccccaa aaattaaatt ttggaaatag
     3721 agttagtgag aaggatagga taatagtatt atgttgattg ttttaatttc agttattgtt
     3781 gcattactgt atcataaaat tacagagtat gtcaacaaca ggaaaattga tattgttatt
     3841 tttattatat ctaagcatgt ctgctagtcc aattattgga ctagtgattg gatatactaa
     3901 aataaatcta ataaattcta ttattatagc tcttggtatg atattggcat taattttggt
     3961 attatcaaga gtgtatagaa aatattttta tagagacgat gatgactaaa gtttttatca
     4021 ataatttggg ctccttgtca actgtagtgg gttgaataaa agctaacgtc tggggaaggg
     4081 tttaaagctt tgctttatca aatattgact tacagagttg ctctattgta ttgatgcatt
     4141 cttagtaaat aatatggtgt tttgaatgaa agatgatcag aaatatttat tagcgggact
     4201 ttattcgctt ctagttgcaa tattttattt tccattgatt gattccaagg ggatttttgt
     4261 aagtatattg atggcggttt tacttttata tttaatatac ttcattgcaa cagtcattca
     4321 tattgtgata attaagttta taagaaaaaa atcttttaag tatttggttc tatatccatt
     4381 tacttatgat gggagctgga gatttcaacc tataaactta ctctactttc cagaaatggt
     4441 gagagatgtc attccgatta atttagttca agaatattgc caaggacaac cttacggact
     4501 gttaaaaaag atgctgaaaa gaattcgatt gtcacgagag atatctttac tcctggctac
     4561 cattatcgtc tattttttta cgcatagaat tctacctttt tcagtattta cgttcatgtt
     4621 ttcttatata ttgctatttg tacaatctta tttgggtggt aataccgctt ggattggaaa
     4681 tagaagatta ataatagatg atgaatttga aaaaatcttg ctttcaaaaa gttatataaa
     4741 agaaatatca tctgcacgat actcagaata cttgacatgt gaatacaaaa actttacacc
     4801 aatcatattg ttagctattt ttgaaaattt gcttgactcc tacttgcttc agaatcagag
     4861 tgaggttgac ttagatattt tttataaagt tttacctttg ctttataaag aaaaatatac
     4921 tatgggattc aattactttg taagtttaaa ttatttacta tataaggttg gatttttggg
     4981 aatcatttac gataatgaag cattaagaga tttgtcaaaa caatatttaa acaaaaatat
     5041 ttcggaatta caagatggtt ctttcgaggg cggtatacaa gatgctgttg cctctaaaca
     5101 aatagtagtt ataaatgaat ttatagcttg tttaaattcc agatgcatgc catcccaata
     5161 tgatagattt ttttataaag atagacctta tattttttcc agaaagagtc ctattaaggg
     5221 gtgaaaattt gttacatttg gattaacaag tgtcctaggg attagaaata actctagtat
     5281 agctagatat ttttgagtaa ctagaaatat cattagtcta cccatatgat tgccagaaaa
     5341 gatggctgat gaatcttatt tatactgttg cagtaggcgt tgtctaggat aaataagcta
     5401 tcttgatggg ggagattgga ataattttag tagaaacatt aaaggattat ggacaagtag
     5461 cgtaggatta ggtgatgcaa tgtgctaagt tggcttggtg ttaacagtag tattatttaa
     5521 caatggaaag tagactaaat gaaaaataag cgttattttt ttgacacaat tttaataatt
     5581 ttactgctta taagcacgat attttgtgtc agcccagtat ttataaaatt ggacatctta
     5641 ggaactccct ctcatgccat cttaaccttt gtattggcga taccgttatt ctatattttg
     5701 tctcaatgct tacatacttt gctattacta gtttcctcaa tcttttgtaa attaagacca
     5761 atttattttt attttatatt tgtgatcatt ataggagcta ggaaatacta tcgaattctc
     5821 tttcaccaac taatgggatt tagtccagga atagcagtgt tttataagga gagtcaaaca
     5881 actaagaatc tgtttaaatt ttactacttc ttgtacttta caacactaat ttcatactat
     5941 tttttcttta cttttgtgta tgacaaaccg ttgcttttgc ctcttattcc attttctatt
     6001 atcatagcat tagtacaaaa actttataga attgagaatc aacaattatt tctgttaaaa
     6061 tcaaaggtac taacaatact ggaatctaag aagaattgtg agtttaatct tcaggattat
     6121 catgaaattt ggaaattgca atcaaaatct gagttgccat gcgtggcttt atcttatatc
     6181 agtttaatca agccatatct ttcagagagt gtgcgtgaac aaattgattt attagaagtt
     6241 aagcgattta aaaaaataaa taatccaata tcactttatg gtatgttgga tgttataaaa
     6301 ttaaatctat atttacggaa ttataatgaa aaaaataaat atgaatcaat gttaaagaaa
     6361 attttagagg taagaccaga ctttgtttta atcgagcaga atattgatga tagtttaaat
     6421 agctcgcaac cattatcttt atctttagcc atttcagaaa tccaactttt gcttgaagtg
     6481 tatataggga tcaaacatgt ttcaattagg agataaaact agaataatat ttgccattat
     6541 aggggcaagt tgcatattag tcagtatgta tcttattcca cttattaaaa ctgagaataa
     6601 attttgggaa atacttttat ttatattagg tgtattgcta attggaattg cgttactaag
     6661 tcaaaaaaaa taaattttga aatagtatta gtgagttcgc aatcatttat tgctagtggt
     6721 ttagaactgt taagtttcaa acaataattg attttaaacc ttaggtaacc tataaaaaca
     6781 aataaggtag agaaaggata tcttatctag gtagctaagt gataaaaaca cagtcgtcta
     6841 tcacttgtta ggaaactatc ctaactatgc ttaataaaaa aggagaaaat aaatggaact
     6901 cgtattacca aataattatg ttgctcttga gcaagaagag atgatgtatc ttgatggggg
     6961 attttctatt ctgagatggc ctgttgcaac agccattaat atagctttta atggtgtttt
     7021 aggtggagga gcaatcagtc tagttagaaa ttatattcgt aattatggtt tgcggcgagt
     7081 tacaagcgca attgctggag cagctgcaag atatgttggg gtacgagttg caaatagagt
     7141 ggcaggattt gcactgtctg ctattaatag atttgcagct tggatgtcaa ttggcgatgc
     7201 tattacaaca atctgggcca acaatgatgt aaataggaga gacccaaatt taaacgcctt
     7261 gtggtaacta gatatgggta ttaaaatgga aaaaattttt gttattattt tttttgtatg
     7321 tctatttata tcatccataa cttttttagc ctatgatttt gttagcgaag aaataaaaaa
     7381 gttgattatt tggatgaatg tcgttttttt gattttaatc atagcaatga taatttatcc
     7441 aaaattaaga aaatgataag gacactgtaa aattatgata cgtaaactaa tcattttttt
     7501 attaatgctc cctctttttg tcttgtggct agagttgcat gtcctagtga ataacttact
     7561 attgaatcta gaatctcccc ttgattttgt gattagtatg agtcttgctt tctgtagttt
     7621 gattttatca aaaatagttt tagacatact atacgcattg aaagatttgt acaagaaaga
     7681 agctcttatt acgatttttc cttttatttt tataggcagg aagaaagtaa atgtgaggtt
     7741 ctcgccctat ttttcattcc atcgtaaaag tttgtcgcct gatgatttaa gatctaggat
     7801 tatatggagt tttattcttg aaattgctat tatcctagta tttattttaa aaattccttt
     7861 tgtcatcatt atgcttacta caatattttt ttggactata atggatatca atcatctcgt
     7921 ttttaataag acggaatttc ttttcaatca aaataaatgg gaaaaagaag attcgtttga
     7981 aagtgatttg acaaatacgt taaaggataa aatacaaaaa tcagatctca gttattctga
     8041 cttaatgtct cttcagctgt atgatgcgat gaaccaatcc acctttttaa cggatagtga
     8101 actttttgag gatattttga aaaaaatcga agattctcat aacacccttc tgtgtacagg
     8161 tcttgtcgag ttactgctgt atgaaatgtc tattagtaac aataacaact ggtccaacaa
     8221 ggttgataaa attaggatac acctcatcag aatcaatcaa ttagattttt tctattatac
     8281 aagttggtta aggcaaaatt ttgatttctg tatgaataga gagtatcata aaatgaaatc
     8341 tcggaagtta ttactgagta ataaaaagat agtttaagtt catcctattt gtaaaacaaa
     8401 tgaaacaatt tcaactaagg aggagaaaac aaatggaact tgtattacca aataattatg
     8461 ttgctcttga gcaagaggag atgatatatc ttgatggggg ggcttattta agcaagcgtg
     8521 cttgtcaagg aatttgcgca gctttagcta tgagtccagg aacttttata gcattagctg
     8581 gagctgcagt tttaaccaaa aaactaataa actatattaa ggttggaggt cttggaggct
     8641 ggcttattgg tgcagcagca ggtgtattgg ctacagcagc agggaaaata gcttactgta
     8701 ttggatatgg tgttcttaat agaggttgtg atattaacgg gaacccttat ccttgggatg
     8761 gatttatatc tgcgacagta agatgaataa tgtagataag attcgaaaaa tacatattat
     8821 cgtatgttgg atgtatatat ttttatcatt cagggcgatt ataaatgaca cggagtattt
     8881 tttgcttatt tttttagctt ttatctactc aatagtatca ctacctctat attctgtgaa
     8941 aaataaaata gtatctatct gtctagccat aaattcgatt ctgttaatga gtttccccat
     9001 tttaatcaat aagtttttcc cagaaagttt tttgacttat accgtattaa taagtgtttt
     9061 tatcctagag ttagcaatct ttcatttaat tggggaagat tttgctacta aattgactaa
     9121 cgaatataag aaaattagtc agtttagaag caaggtgtct caatctcctt ggataaaata
     9181 tttagagatt tctagtttta tattaactat atttccatct attctttacg gtacagttga
     9241 taatcgtgta ttggctctta tctttttgat aaaaatttgt gtagatacta cgataaaatt
     9301 tttattcatc agattatttg acacaagtac tttaatgaag aggagaatat tttttctttt
     9361 cgcattggat gttatagctt acttattttt aggatatctt ttagtgattc aaaaagcagg
     9421 ctatttgttt tcggttttac ttcttttttc taatttttca gttccattta tcaaagcaaa
     9481 ggaatacgaa ttatttaaaa atagcaagta atagaaacat aggttaaatc ttagaacggc
     9541 ttaaaatcag atatgataaa gggaaatagt cttatctagg taactaagtg ctacaaacac
     9601 agtcgaggat cacttgttag gattacattt taatcatgct taataagaat ggagaattcc
     9661 tatggggatt gtattactct gaagttttga ataccttagt tctcgtgatt ttgagctatt
     9721 ctagctttag aatccttcag aacgtaaaat tttaagcagt caaagactta gaatagcccg
     9781 aaaacattta ttttatagca attctaggct tagaattgct catatatatg tatttgagct
     9841 gctcgatagt taggacagct ctattctgag aaatctgacc tatcaatgct tcagaatagt
     9901 atttcgccaa tggtttttat tgaagaggtg attgttttat gatagtctaa aataaaactg
     9961 atttaatgga gtaaatggag aatcgttaca tgaaacattt ttttgcagga attgatgaga
    10021 taaaacttgt tggctactta ttgtatatat gtgtaggact agcccctctt tttcatatat
    10081 atgtcatagg ttcggaactg agttttgtga aaatagtgtt atctattttg ggagttatat
    10141 ttgtaagtat gttaactatt gctcgaattt atcggacttt tctataaaga atagtttgga
    10201 cttgaactgt atctaaaaac agaggttggt cgattatcag cctctttctt gtcgttgaaa
    10261 agagtgagat atattgactt ttgaaaaaaa tgtcataatt gagttgggat aaacctaaaa
    10321 ataatatagt agattgaatc tggaatagtc cgttatgact gttaaaatat ttctagaaat
    10381 taatttgact ttcctaatcg atttgttcac atcttgtttc aatctactat actttctcgt
    10441 taaaccgttc ggcaattcat ggtgacatgg acacatagta gtaagcacac tatgtggctt
    10501 ggcagagcta aaaactgttc ccttgcgata agcctaataa gcacaacata gggagttaga
    10561 gaagccgact ctaatcaacc actttgggca atagtgagaa ctgccccgtg cttttttatt
    10621 ttgagaaaat atggagtttg tcgttgaaat tacttgattg tattttggac tatcaagaaa
    10681 gattcaatgg aaaaacatgt caagtatcaa cgaattataa gtatttagag attttcaaag
    10741 tgaatttttg tttgactgat ttacatcatt tatttgactt atacaaaatc acacgagatt
    10801 atgctagtca aacaaaacct gctattcaag ctggtgtttt tattttagaa gattttagaa
    10861 atatcctatg tacaatgatg taatcgaaag gatatcttta tatgagttta tcggtgatat
    10921 tttctattct aaaataataa gttgttgcat cgtagctagc gatttatcta aaaatactat
    10981 gaaattggac gtcatatttt ttgaggataa aaataaaaga tccgcagttt taggtttacg
    11041 aagagacaaa agcggagtat ttaaaccagt tactctacat tttacaagcg ctaagaaata
    11101 tgttaaagtt cgtaaaacag atgtgaaaga aatgaaatgg ctataaagat tgtccgcagg
    11161 cttaagcttg cgttttttgt tgtttaacga aattaaaaaa caagctctct catcggaagt
    11221 gtttttatag gaatgaagga agatgatgct atgtttaaaa agagaatcga aaagggcaaa
    11281 agtagtgtct ttatcttttt ggagtagaag cttatgatat agaaatttgt aataaaaatg
    11341 taatataaaa catttgacaa atatttacgg ggggagtata cttaatataa gtatagtcta
    11401 aaaatgacta tcagaaaaga ggtaaattta gatgaataag aaaaaaatga ttttaacaag
    11461 tctagccagc gtcgctatct taggggctgg ttttgttacg tctcagccag taactgtgaa
    11521 agcggaggtg gcatctacag catctgcttc atctaccgta gaacaggcaa agagcaaggt
    11581 tgatgaagct cagaaaaatt acaatgaagc tcagaaaaat tacaatgaag ctcagaaaaa
    11641 agtcgaagaa aaggcaaatg ctgttaaaaa attagatgaa gaagtacaaa aagctaatga
    11701 agcggtgcaa agagcttatt tggaacaaag aaaagcacaa gaagaaaaaa ataaacatcc
    11761 aagaaaaaaa gagtacatta aaaaattaga agaagcaaat aaaaagatag atgaagaaaa
    11821 agacaaacag aaatctaagc aaacagcata tacacaaaaa atgtctgagt ctattcctga
    11881 tgtcaaccct ctgttgacaa gattaaaaac cgaaaaagac aacctcgaag ctgccaagcg
    11941 acaacttaaa gaagctaagg cggctgaaaa gactgagaaa gaagcaaagg ttaaaaaagt
    12001 agaaactgag cttgcccaag caaaggaaaa agttaagaaa caagcggaag aagatcgccg
    12061 taactaccca accaatactt acaaaacgct tgaacttgaa attgctgagt ccgatgtgaa
    12121 agttaaagaa gcggagcttg aactagtaaa agaggaagct aacgaatctc gaaacgagga
    12181 aaaaattaag caagcaaaag cgaaagttga gagtaaacaa gctgaggcta caaggttaga
    12241 aaaaatcaag acagatcgta aaaaagcaga agaagaagct aaagtaaaat tagaagaagc
    12301 tgagaaaaaa gctactgaag ccaaacaaaa attggatgct gaacgtgcta aagaagttgc
    12361 tcctcaagct aaaatcgctg agttggaata tgaagttcag agactagaaa aagcgctcga
    12421 agagattgat gagtctgaat cagaagatta tgctaaagaa ggtttccgtg ctcctcttca
    12481 atctaaattg gatgccaaaa aagctaaact atcaaaactt gaagagttaa gtgataagat
    12541 tgatgagtta gacgctgaaa ttgcaaaact tgaagatcaa cttaaagctg ctgaagaaaa
    12601 caataatgta gaagactact ttaaagaagg tttagagaaa actattgctg ctaaaaaagc
    12661 tgaattagaa aaaactgaag ctgaccttaa gaaagcagtt aatgagccag aaaaaccagc
    12721 tgaagagcca tcgcaaccag agaagccagc tgaagagcca tcgcaaccag agaagccagc
    12781 tgaagaagct ccagccccag agcaaccaac tgagccaact caaccagaaa aaccagctga
    12841 acaacctcaa ccagctccag ctcctcaacc agagaaaaca gatgatcaac aagctgaaga
    12901 agactatgct cgtaaatcag aagaagcata taaccgcttg actcaacagc aaccgccaaa
    12961 aactgaaaaa ccagctcctg caccacaacc agagcaacca gctcctgcac caaaaacagg
    13021 ctggaaacaa gaaaacggta tgtggtactt ctacaatact gatggttcaa tggcgacagg
    13081 atggctccaa aacaatggct catggtacta cctcaacagc aatggcgcta tggcgacaga
    13141 atggctccaa tacaatggct catggtacta tctaaacgct agcggcgcta tggcaacagg
    13201 ttgggctaaa gtcaacggtt catggtacta cctcaacgct aatggtgcta tggcgacagg
    13261 atggctccaa tacaacgatt catggtatta cctaaacgct agcggcgcta tggcaacagg
    13321 ttgggctaaa gtcaacggtt catggtacta cctaaacgct aatggtgcta tggcgacagg
    13381 atggctccaa aacaacggtt catggtacta cctaaacgct aacggtgcta tggcaacagg
    13441 ttgggctaaa gtcaacggtt catggtacta tctcaacgct aacggttcaa tggcaacagg
    13501 ttgggtgaaa gatggagata cctggtacta tcttgaagca tcaggtgcta tgaaagcaag
    13561 ccaatggttc aaagtatcag ataaatggta ctatgtcaat ggcttaggtg cccttgcagt
    13621 caacacaact gtagacggat atactgtcaa tgaaaacggt gaatgggttt aagccgatta
    13681 aattaaatca tgttaagaac atttgacatt ttaattttga aacaaagata aggttcgatt
    13741 gaatagattt atgttcgtat tctttaggta cctgtcttat gatttcagga aatgtcatta
    13801 aaaaaacgac tccttttctc taacttggaa aatagattag agaaaatggg ttgttttatt
    13861 tattatagtt atttgaatga agataagaag aaggtatatt cacatcattc acataatctg
    13921 tatattgact ataagtttta aaaaacaatt tttaagctct tccttgtctt ctccaaccaa
    13981 gcgtgttata atgaatactg ctcaagcgac cttcaatcgt gaagcacaca cgaccttcaa
    14041 tcgtgaataa acgaatagat gggagactta ccatgagtga taactctaaa acacgtgttg
    14101 tcgtggggat gagtggtggt gttgattcgt cggtgacggc tcttttgctc aaggagcagg
    14161 gctacgatgt gatcggtatc ttcatgaaga actgggatga cacagatgaa aacggcgtct
    14221 gtacggcgac cgaagattac aaggatgtgg ttgcggtggc agaccagatt ggcattccct
    14281 actactctgt caattttgaa aaagagtact gggaccgcgt ttttgagtat ttcctagcgg
    14341 aataccgtgc agggcgcacg ccaaatccgg acgttatgtg caacaaggaa atcaagttca
    14401 aggccttttt ggactatgcc ataaccttgg gggcagacta tgtagcgact gggcattatg
    14461 ctcgagtggc gcgtgatgag gacggcactg ttcacatgct tcgtggcgtg gacaatggca
    14521 aggaccagac ctatttcctc agccaacttt cgcaagaaca acttcaaaaa accatgttcc
    14581 cactaggaca tttggaaaag cctgaagttc gaaaactagc agaagaagca ggcctttcga
    14641 ctgctaagaa gaaagactcg acagggattt gctttatcgg agaaaagaac tttaaaaact
    14701 ttctcagcaa ctacctgcca gctcagcctg gtcgtatgat gactgtggat ggtcgcgata
    14761 tgggcgagca tgctggtctg atgtactata cgattggtca gcgtggcggg cttggtatcg
    14821 gtgggcaaca cggcggtgac aatgcccctt ggttcgttgt cggaaaagat ctaagcaaga
    14881 atattctcta tgtaggacaa ggattctacc atgattcgct catgtcaact agcctagaag
    14941 ccagtcaagt ccactttact cgtgaaatgc cagaagagtt tacgctagaa tgtacggcta
    15001 aattccgtta ccgtcagcca gactctaagg tgatagtcca tgtcaaagga gacaaggcag
    15061 aggtcatctt tgcggaacca caacgcgcga ttacaccagg acaggcagtt gtcttttacg
    15121 atggcgaaga gtgtctaggt ggcggtttga ttgacaatgc ttaccgcgat ggacaagttt
    15181 gtcagtacat ttagattgac aaattttctc aatttgctac aataataaca gcaatagaaa
    15241 tgatggtcaa agctcatgga tgttgcaggc ttttttgtcc tgcacttctt tgtagttttg
    15301 actgtttttg tgtcgtttaa gggaaaggac aagaatgact caacaagact ttcggacaaa
    15361 agtagacaat acggtttttg gtgttcgggc gacagccttg attgtccaaa atcacaagct
    15421 tctagttacc aaagacaagg gcaagtatta cactattgga ggtgcgattc aagtcaatga
    15481 aagcacggaa gacgcggtag tccgtgaagt gaaggaagaa ctgggtgtta aagctcaagc
    15541 tgggcagcta gcttttgtgg ttgaaaatcg ttttgaacaa tacggtgttt cctatcacaa
    15601 tatcgagttt cattatctgg tggatttgct tgaagatgcc ccattgacca tgcaggaaga
    15661 tgagaaaagg cagccctgtg agtggattga cttggataag ctccagaata tccagctagt
    15721 tccagccttt ttaaaaacag ccctaccaga ttgggaaggc caactaagac acattcatct
    15781 tgaggaatag gagagaaaca tgacttatca ttttactgaa gaatacgata ttattgtaat
    15841 tggtgcggga cacgctgggg ttgaggcttc cttggccgct agccgtatgg gctgtaaggt
    15901 cctgcttgcg accatcaata ttgaaatgct ggctttcatg ccttgtaatc cctctatcgg
    15961 tggttctgcc aaggggattg tcgtgcgtga agtcgatgcc ctcggtggcg agatggccaa
    16021 aaccattgac aagacttaca tccagatgaa gatgctaaac acagggaagg ggccagctgt
    16081 ccgtgccctt cgtgcgcagg ctgacaagga actttactct aaggagatgc gcaagacggt
    16141 tgaaaaccaa gaaaatctga cccttcgtca aaccatgatt gatgagattt tggtggaaga
    16201 tggcaaggtt gtcggtgtgc gtacagccac ccatcaagaa tatgctgcta aggctgttat
    16261 tgtgacgaca gggactgctc tccgtgggga aattatcatc ggagacctca agtactcatc
    16321 aggtcctaac cacagcttgg cttctattaa cctagctgac aatctcaagg aactgggtct
    16381 cgaaatcggt cgtttcaaga caggaacccc tccacgcgtc aaggcctctt ctatcaacta
    16441 cgacgtgaca gaaatccaac caggagatga agcgcctaat cacttctcat acacttcacg
    16501 tgatgaggat tatgtcaagg accaagtgcc atgctggttg acctatacca atggtaccag
    16561 tcatgagatt attcaaaaca acctccaccg tgcgcctatg tttacaggtg tggtcaaggg
    16621 agtggggcct cgttactgtc cgtcgattga agacaagatt gtgcgctttg cggacaagga
    16681 acgtcaccaa ctcttccttg agccagaagg gcgcaatact gaggaagtct atgttcaagg
    16741 actttcaacc agtctgcctg aggatgtcca gcgtgacttg gttcattcca tcaaaggttt
    16801 ggaaaatgca gagatgatgc ggacaggtta tgctattgag tatgatatgg tcttgcctca
    16861 tcagttgcgt gcgactttgg aaaccaagaa aatctcaggt ctcttcactg ctggtcagac
    16921 aaatggaaca tcaggttacg aagaagctgc tggccaaggg attatcgcgg gtatcaatgc
    16981 ggctctgaaa atccaaggca agcctgaatt gattttgaag cgcagtgatg gttatatcgg
    17041 ggtgatgatc gacgacttgg tgaccaaggg aaccattgaa ccctaccgtc tcttgaccag
    17101 tcgtgctgaa taccgtctca ttcttcgtca tgacaatgct gatatgcgct tgactgagat
    17161 gggacgcgag attggccttg tggacgatga acgctgggct cgttttgaaa tcaagaaaaa
    17221 tcaatttgat aatgagatga agcgcctaga cagtatcaaa cttaagccag tcaaggaaac
    17281 caatgccaag gttgaggaga tgggcttcaa acccttgacc gatgcagtga cagccaagga
    17341 attccttcgc cgtccagaag tttcttacca agatgtggtg gccttcatcg gaccagctgc
    17401 agaagacttg gatgacaaga ttatcgaatt gattgaaaca gaaatcaagt atgaaggcta
    17461 tatttccaaa gccatggacc aggttgccaa gatgaaacgc atggaagaaa aacgcattcc
    17521 ggccaatatc gactgggatg acattgattc tatcgcaacc gaagcccgtc agaagttcaa
    17581 actcatcaat ccagaaacca tcggccaagc cagccgtatt tcgggagtaa acccagcaga
    17641 tatttctatt ttgatggtgt atctggaagg taaaaatcgt agtatttcta aaactcttca
    17701 aaaatcaaaa tgatacgtcg tcggcttctt acgaatgagt tcaaagcttg gctttgattc
    17761 atctccagcc tcccatagtt tcccgaacta tgagagctaa ctcaagttat gctttcgctt
    17821 agccttctag tctgatttcg atttttattg atactcttcg aaaatctctt caaaccgcgt
    17881 caacgtcgcc ttgccgtagg tatggctact gacttcgtca gttctatcta caacctcaaa
    17941 gcagtgcttt gagcaaccta cggctagctt cctagtttgc tctttgattt tcattgagta
    18001 tgagttttaa tctcaaaaga aagcatagag aaaaacagct ccgaggacgg ggctgttttc
    18061 ttgctttatt cttcatctgg tgtgaggatc atcgggatga tgatcggttc acgttcggta
    18121 ttttcataga ggaaggggcg aatagcgttg acaatggcac cattgacaga ttgcacgctg
    18181 gcatccttat ttttcagtgc gatacgaatg gcattgaaga ggatacgctg gctttggcga
    18241 atcaagtcgc cagactctct catgtagaca aagcctcggc tgaggatgtc tggaccagac
    18301 agaatcatct gcgatttgaa gtcaacagtt gcgactgcca gaacgacacc gtcttcagat
    18361 agatcgcgac gatctttgag gacagctgcg ccaatttcac cgatacgatt tccatcgaca
    18421 tagatatctt gggcgttgaa atgacctgcg atacgagctg agtcagcagt aagggcaagc
    18481 acatcgccat tgctcatgat aaagatattg tccttctcaa caccagtatc cactgctagt
    18541 ccagcgtgga ctttttgcat gcggtattca ccgtggacag gcatgaagta ttttggctta
    18601 atcaagcaga gcatgagttt ttgctcttgc tgaccaccgt gtccagatgt atggatattg
    18661 ttcactttac cgtggataac ttcgacacca gcttcagaaa tgatgttaat cagcttgttg
    18721 acgctagtag tgtttccagg gatgggacta gaagagaaga taacggtatc acctggttgt
    18781 aattgtactt gacggtgggt tccgttggcg atacgagaga gagctgccat aggctcaccc
    18841 tgactacctg tacagaggat aagaacttct cctgcaggat aatctttgat ttcatttggc
    18901 tcgataaagg ttcccttagg agctttgatg tagccaagat cgattccgtt gacaatggcc
    18961 ttttccatag aacgaccaaa gaccgcaatc ttgcgtccag tcttaacagc agcttctgtt
    19021 gcctgctgga gacggaagat atttgaggca aaggatgcaa agatgatacg tccttcaata
    19081 ccttggataa tcttcataat ggactgacca acgacttttt cagagttggt aaaggttggt
    19141 acttccgcat ttgtcgagtc agacaggaga cagagcacgc cttcttcacc aagcgcagcc
    19201 atacgatgca agtccgcagg ttctccaact ggagtaaagt cgaacttaaa gtcacccgta
    19261 cagacgattt tcccttgagg agtatgaatg acaatcccca aaggctctgg aatagagtga
    19321 gtcgttctaa agaaagttgc cttgagattt ttaaaggtca actcggtgtt gtggttgatt
    19381 tcgtaaagtt tggcgttgcg caagaggccg tgttcttcga gtttcccacg gatcaaagcc
    19441 aaggcaagcg gtccagcgta gatagggaca tttgcttgct tgagtaggaa cggaatccca
    19501 ccaatgtggt cctcgtgtcc gtgtgtgatt aaaacagcct tgacgcggtc gatattgtcc
    19561 acgatgtaag agtagtcagg aatgacatag tcgataccaa gcaagtcatc ttctgggaat
    19621 ttaatcccag catcgacgat gataatctcg tcttggtatt caattccgta agtgtttttc
    19681 ccgatttctc ccagaccacc gatggcaaaa acgccgactt cttcaggttt aagagtatag
    19741 gccatattag aactccgtga tttcgaaggc gccagtttct ttttcgtaat cgagcaattt
    19801 gtcagacaag agttcgatat attcgatatt gtactctggg cgattttctt cgacaagttg
    19861 gcgagcagtg atgcgaccct caagttctga gctggcatcg atgtctaggt aaagtgcgcg
    19921 tgttgtttca cggcgtgggc tacgttcttt tgtttcttga taaaaaactt tgtaaatcat
    19981 atagttcctt tctatttaca ggtcagctta ttccaaactt ttagcagata tttgcttgat
    20041 ttcattccat tttgtgtcta gattgacctt gattcgttac aattatttca attataccac
    20101 aaaatgaaaa attagtaaaa gacggaaacg agaaagttag gggacgattt ctttggtaag
    20161 cgtttgcaag aataaagaaa atgtattatg ttgataacgg aagtgaaatt tattgcttgt
    20221 cataattcca tatagatttt aagtcaagga ggttgcccta tgtataactt atttgctttt
    20281 gctatcggag ctcgcttggt agcttttatt gtttttgttg cttgcgttta tgcggggaat
    20341 cttctatttg ccaaactgga acgacgacaa accaagttct tgtggaaaat gacctttgtg
    20401 actctctact cgctttttct cctcctcgtg acctatgtcg tttggtttgt attttacttt
    20461 gaattaaatg cttagatgcc ctgcttatgc tggggttttt tgtttgggga tgtagagata
    20521 gtagatttac gacacgaaaa ttccacccca tctatcaaga tgaggtggtc tttgcatttt
    20581 tctaacatga catgacaatg gaattttcaa agcttattat ttttatggga gtcattgttc
    20641 tgatcaaaaa tagagttgaa gcataaaaat aaaacaatac aaacagataa atcgaatggt
    20701 aaagggatgg tagtaatgtt caaatcattg agaatgctga gcaaactgta agagatgaag
    20761 acaataaaat ttatccttct aaaaatgtgt ttcttatcta gcatgactta ccaaactttt
    20821 tacgaaggtc ttttccaatg gtataacctg ccataaataa agcgatacca acaccgacac
    20881 caaagccacc cttgatttct tgcaattcct tattagaaat ttcacgataa ttgttcaaat
    20941 ctttcatcat attaatctcc tcctaaaaaa taaccagctg ccaaaccaga acctataagt
    21001 gctgttccac ctgcaatagc cttccaagat actgctactc caaagataac caagggagcg
    21061 agaccgccat caatatctga taattcattt tcagataagg ataaaaattg attgtcaaga
    21121 atgtcaaaat ttgtcattga actttcctcc ttttttcttg ttcactttta tattcggaaa
    21181 agaaaaggaa aagtgagcat ttttttaaat tatttttagt taagtgaggg cactcttgtc
    21241 tgggggcaaa gaaaattccc atgtgccagc ttttgtagta taatagtaag cagacaaaaa
    21301 gataggaatg tgttatgaaa gtattagctt ttgatacgtc cagcaaggct ctttctctgg
    21361 ctattttaga ggataagcag gttcttgccg agacgacgat taatattaag aaaaatcaca
    21421 gtattactct tatgcctgcc atcgattttt tgatggcaag tttggattgg acacccaagg
    21481 atttggaccg aatcgtggta gctgaagggc cgggtagcta tacaggcttg cgaattgcgg
    21541 tagcaactgc taagacctta gcttacaccc tgaacattga gttggttggt atgtctagtc
    21601 tcttggctct agtgccatat caacaagaag gtttgtttgt tcctttgatg gatgcgcgtc
    21661 gcaataatgt ttatgcagga ttttatgaaa atgccaaacc tgtcatggca gaagcacatc
    21721 tgccttttga gcgagtcatt gagctaatca agggcgctag tcaggtaacc tttgtcggag
    21781 aagttggccc ctttgttgag cagattcaaa aacacttgcc aaggactgat tacaaagaaa
    21841 cattgcccaa tgcagctaat ctagctcttt tggcctggga caaggaagca gactccttgc
    21901 atgattttgt gccaaattac ctcaaacgtg ttgaggcaga ggaaaactgg ctcaagaacc
    21961 ataccgagtc tggtgagtct tacattaaac gcctatgata gaaatcaaac gtatccaaca
    22021 gcaacctgcc ttggctcaag atatctacgc tgtcatggca gctgtttacc cagtcagtcc
    22081 ttggacacta gaacaaatcc aagcagatct gtcccaagac cagacttggt atgctctggc
    22141 ctatgatggg gcagaagtga ttggctttct aactgttcag gagactctct ttgaagcaga
    22201 agtcctgcaa atcgctgtca aaggagcctg tcagggtcag ggaattgcgt cagccttgtt
    22261 tgctcaattg ccgacagaca aggaaatttt cctcgaagtt agacagtcaa atcaacgagc
    22321 gcaagcattt tacaagaaag aaaaaatggc ggtcatcgct gagcgaaaag cctactacca
    22381 tgacccagtc gaggacgcca ttatcatgaa gagagaaata gatgaaggat agatatattt
    22441 tagcatttga gacatcctgt gatgagacca gtgttgccgt cttgaaaaac gacgatgagc
    22501 tcttgtccaa tgtcattgct agtcaaattg agagtcacaa acgttttggg ggcgtagtgc
    22561 cagaagtagc cagtcgtcac catgtcgagg tcattacagc ctgtatcgag gaggcgctag
    22621 cagaagcagg gattacagaa gaggacgtga cagctgtggc ggttacctat ggaccgggct
    22681 tggtcggagc cttactagtt ggtttgtcag ctgccaaggc ctttgcttgg gcacatggac
    22741 ttccgctgat tcccgttaac cacatggctg gccatctcat ggcggctcaa agtgtggaac
    22801 ctttggagtt tcccttgcta gccctcttgg ttagcggtgg gcacacagag ttggtttatg
    22861 tttcggaggc aggagattat aagattgttg gggaaacccg tgatgatgcg gttggtgagg
    22921 cttatgataa ggtcggccgt gtcatgggct tgacctatcc tgcaggtcgt gagattgacg
    22981 agctggctca tcaggggcag gatatttatg atttcccccg tgccatgatt aaggaagata
    23041 atctggagtt ctccttctca ggtttgaaat ctgcctttat caatctttat cacaatgccg
    23101 agcaaaaggg agaaagcctg tctacagaag atttgtgtgc ttccttccaa gcagcagtta
    23161 tggacattct catggcaaaa accaagaagg ctttggagaa atatcctgtt aaaaccctgg
    23221 ttgtggcagg tggtgtggca gccaataaag gtctcagaga acgcctagca gccgaaatca
    23281 cagatgtcaa ggttatcatc ccccctctgc gactctgcgg agacaatgca ggtatgattg
    23341 cctatgccag cgtcagcgag tggaacaaag aaaacttcgc aggctgggac ctcaatgcca
    23401 aaccaagtct tgcctttgat accatggaat aaagagtggc tctttgtcaa gtgtagtggg
    23461 taggcgaaaa gctacaatct ggagagtacg aaattcgtac tctctttttt gatgttcaaa
    23521 gtcatcaaaa tccgtttttt aaaattgtta aagttccgga aaccaaaggc tttgcgcttg
    23581 ataatcttaa tcaaattatt ggtcgcttca agtttagcgt tggaataagg cagcttaagt
    23641 gcgttgataa tcttatctct atcccttaaa aaagtccaaa agaccgtttg aaaaagcgga
    23701 tgaaccgttg gcagttcttg ctcaatcaat ccaaagaaat ggtcggcatt cttctcttga
    23761 aaatgaaaga gcaggagttg atagagttcg tagtgaacct gtaatccctc agagtaagaa
    23821 agcagcttat ctcgtacttc ccgatgggtt aagtgcattc gaaacgtggg ctgatagaat
    23881 cgtttctgac agagcttgaa gctatactgg tgtagtaaat cagtccagtg gactgattta
    23941 gcccgagcct agaaacgaaa aagcgagggt tccaaaagcg cttgagcgcc cgatactcca
    24001 aggattttcg gtcaaattgg ttcatgattt ggattcgtac tcggttcata gctcggctca
    24061 gatgttggac aatgtggaag cggtcaagaa caatcttcgc ctttggaaag agttgcttag
    24121 cgagccgata ataagggctg tacatgtcca tggtgatgac ctcgaccagc tcccgaacct
    24181 ctctctgata gcgttggaaa tggtttcgaa tcaccgcatg agttcgcccg tctaaaattg
    24241 cgaggatgga tttggactca aaatcttggg caatgaagct cattttgctc tttttgaagc
    24301 tatactcatc ccaactcata acttttggca acttggtcca atcggtttca aacttaaatt
    24361 ccctcagttt tcggatgacg gtggaagttg agaccgccaa tctgtgggca atctcagtca
    24421 tagactgctt ttcaaggagg agctgagcga ttttttgtct caccatgttg gaaatctggc
    24481 aatttttctt gacaatggat gtttgagaaa cgaccacctt aaggcaattc ttgcactgaa
    24541 agcggcgctt tttgagatgc agtaccgtgg gtaaaccctg acagtcgaga agcggaattt
    24601 tagaggcttt ctggaagtcg tatttgatca tcttcccttg acaatgagga caaggaggag
    24661 cgggggaatc caactttgcc tgaacgacta gatgggtttg gtatttcaga acagacaaga
    24721 ttttgatatt tttgtcttcc aatccgagca aatctgtggt attcttaagt tgttccatat
    24781 gaaccctttc taatgagttg gttagacgct tttcattata ggtcatatgg ggcttttttt
    24841 ctacaagaaa cgaccctata attcctgggg tgggattacc cactacagaa attatagagc
    24901 caaagcattc caaagtcttg tctgactctg gaatgttttc tgttcacagt tgttttgtaa
    24961 ctgcctaata agtaaccgcg ctataacgag gtatctaccg agattgtcaa ttctccagta
    25021 tactgttata aaaaacaaaa ctggaggatt tttttatgtc cccccatcgt cccatcgact
    25081 gttccgtagc tgcccaataa cggagtatag tcggctgaaa caagaatagg acaaaagtgc
    25141 ctcaagaaaa gcatcgtaat ttgacaatat tattctgaga tgtttttgat gtaagcccat
    25201 attttctcaa tgggattata ctcgggtgaa taggtaggaa gtggtaacag tctatgccct
    25261 gctccttaca catgttcatt ctgtgaaacc ttgcatggtc cataatgata agggatggtg
    25321 tatctaaagt aggtagtaag aatttttgga agcaagcttc gaaaaagtca ctcgtcatcg
    25381 tatctttata ggtcatcgga gccatagtat attgaaacaa aaaagggcat tagtacttct
    25441 ataacagtat cgcaatctct ggtgtgcgtt gtctcgatct taattcaaat aattatatct
    25501 tcttccagag agactaactg cctgctcaag gctcgatcat attcgagata aaaataggtc
    25561 tcaacccctg tctcataaat ataaatagga gtcaggtagc ttaagtgatt caattcttta
    25621 aggaaccgat ttactttttc tgggtcttgt tcgcagtagg tacggctctt ttttagacta
    25681 tatcccatag cctttagagc gtaatgaata gttgttggag gacagttgaa ttcagcagct
    25741 atttcagtca aataagcatc gggatgagtt tcaatataga tttttaattt atctctatca
    25801 attttttcaa ttaatagtgg aagttttctt gaagttagtg tattgaaacg tttttataca
    25861 gtgctaaaat ttgaagtgta gtgcagatgg atttgtaata gtattatagc aattctttgg
    25921 agaaggtcga gacaagataa aataggtatc tttgagatat aagcatgatt tactataaaa
    25981 ttattgagtt attcataaaa atttctctat caaatatcat aatgcgtcgt ttaaggagat
    26041 ggttacaatg caatatagtt gtggaaaaat aaatattaat atccctgatg gatatggaga
    26101 cattaaggat atagtgtttt cggctcatat tatagttagg tataataatg gacattgtgg
    26161 aggtatagat ccgcatataa ttggactttg taaaaagcag atcagaagga tgtctctata
    26221 tcctattttg ataatcgtat ctagagattc aaaggttatt gatgattata aaaatttaga
    26281 tattgcctat gttgattgta ctcaatgttc aaataatttt gaaactgcct tacacgttaa
    26341 aaatatttta aaattattaa aaatccaact tatacactgt catggctact caactaatta
    26401 ttttttatat atgttaaaaa aacttgacaa gaatggattt ggaaaggtta agacagtgat
    26461 aacatgccat ggatgggttg aatataactt aaaaaagaaa ttcttgactt attttgattt
    26521 ttggacatat tctatgggag atgcttttat ttgtgtatct gaaactatga aaaaaagatt
    26581 ggagagtata ataaaaaaat aagaagatag ttgcaataaa taacggaatt aatgtatcaa
    26641 atagcgattt agatgtagta ggtgttcaag actttaaaaa agaattttgt attccaaata
    26701 acaaaaaaat catttgttat gttggaaggt tggatccaga aaaaaggccg gatagattcc
    26761 ttgaatttgc agaaaaatta tttttagtca gagaagatgt gatttttatt atggctggaa
    26821 atggtagtat gtgggctgct ttgaaagaaa aaatttgcca tttgaaatgt agagataatt
    26881 tcaggttact tggtgaaatc tatccagcta ctatagttta ccaaatatcg gatttattgt
    26941 atattccttc tgatacagaa ggaataccta tgtgtgtttt agagtcaatg tcacaaggaa
    27001 ctccggtttt agctagtaat gttggcgggt taagtgaaat tattgaacat agggttgatg
    27061 gatttttatt tgagaaggaa gatgttgagg gagtgtgtgc ttgtgctaat tttttactca
    27121 atgattctga gtatttgaaa tatataggtg agaatagtaa atcaaaaata agaaaacatg
    27181 agagtatatg atgaattatt agagaagagt agtcatggat aggaatatag atcaagaatt
    27241 agtaagtatt ataataccaa cacataatag gtatgaatct ctgattagag ctgtgaaaag
    27301 ttgtttacat caaagttata aaaatatcga agttataatt attgatgata attatagtaa
    27361 tgtaaattta agaaataaaa ttatccatca atttggctat accaatcata gaattaagtt
    27421 aattttaagt aatgaagatt taggtgcaac taatgccaga aacataggta tcaaaaattc
    27481 tagaggtaag tatatatcat ttttagacga tgatgatgaa tatatgccag atcgaatttt
    27541 aaagttgatg gcttgtttta aaaagagtag aatgaagaat ttagctttag tttatagtta
    27601 tggaataata atttatccaa atggtacacg agaagaggag aagaccgatt ttgttggaaa
    27661 tcccttgttt gttcaaatgg ttcacaatat agcaggtacg tcattttggt tgtgtaaaaa
    27721 agaggtgcta gaattaatta atggttttga gaaaatagat tcacatcagg acggtgttgt
    27781 tttattaaaa ctacttgctc aaggatacca aattgatata gtgcgagaat tcttggtgaa
    27841 ttactacgct cacagtaaag aaaacggtat cactggagtg acacaaaaaa caattaatgc
    27901 agatgaagaa tattataatt actgtaggaa atattttaat ttattgagtt tcaacgagag
    27961 aatattggtt acaaagaaat attattcttt aaacataaag cggttactat taataggaga
    28021 caaatgcaag gctttaaaag taatcaagaa ggcaagagaa gaaaaaattt ttaacgaatt
    28081 tctttttttg aaatatatgt tattatatca ttttggtttt ttctattgta tatatgacaa
    28141 ctatgttcaa ttaaaattta gaaagtgaga aactattgtg tatactatta taaaatcaaa
    28201 tataaaaaaa tttagtttat taacgatatt tattgttgct ggtcaattat tgctaattta
    28261 tgcagcaact attaatgctc tggtgttgaa tgaattaatt gcgatgaatt tagagcggtt
    28321 tttgaaattg tcaatctacc aaatgattgt ctggtgtggg ataatattcc ttgactgggt
    28381 agtgaaaaat tatcaggttg aagtgatcca agagtttaat ctagagattc gaaatagagt
    28441 tgccacagac atctctaact ctacctatca agaatttcat agtaaatcat caggaacata
    28501 tctttcgtgg ctaaataatg atgttcagac tttaaatgat caggctttta aacaactttt
    28561 tttagtaata aaaggaattt ctggtactat atttgcagtt gtgactctta atcactatca
    28621 ttggtcattg actgtagcca ccttgttttc attaatgatt atgctacttg taccaaaaat
    28681 ctttgcatcg aaaatgcgag aagttagtct aaatttaact aaccaaaatg aagctttttt
    28741 aaaatctagt gagactatat tgaatggatt tgatgtgtta gcgtccttga atcttttata
    28801 tgtattgcct aagaaaatta aagaagcagg aattttatta aagatggtta tacaaagaaa
    28861 gacaactgta gaaacgttag caggcgctat tagcttcttt ctcaatattt tttttcagat
    28921 atctctcgtt tttttaacag gctatcttgc aataaaagga atagtgaaaa ttggtactat
    28981 tgaagcaata ggagcactaa caggtgttat ttttacagcg ctaggtgaat taggaggtca
    29041 attatcctct attattggta cgaagcctat ttttttaaaa ttgtattcaa ttaatccaat
    29101 tgagtcaaat aaaatgaatg atatcgaacc aaatgaggtg aatagagatt ttccgttata
    29161 tgaagcaaaa aatatttgct ataagtatgg agataaagaa atattaaaaa acttaaattt
    29221 ttgttttcaa cgtaatgaaa agtatttaat tttaggtgaa agtggaagcg ggaaatctac
    29281 attattaaaa ttattgaatg gctttttgag agattatagt ggagaattgc gattctgcgg
    29341 ggatgatata aaaaaaacct cctatttaaa tatggtttcg aatgttctat atgtagatca
    29401 aaaagcttat ttgtttgaag gtacgattag agataatatt ttattggaag aaaattatac
    29461 tgatgaagaa atactacagt ctttagagca agttggtttg agtgtaaaag attttcctaa
    29521 taacatttta gattattatg ttggtgatga tgggagatta ctgtcaggag ggcagaaaca
    29581 aaaaattact ttagctagag ggctaattag aaataagaaa atagtattaa ttgacgaggg
    29641 aacttctgct atcgatagga gaacttcgtt agcgattgaa cgtaagatat tagatagaga
    29701 ggatttgact gtcattattg ttacccatgc tccgcatccg gaacttaaac aatattttac
    29761 taagatatat caatttccaa aggattttat ttaagaatat aggggaggga aacatgagat
    29821 tctattgtga agcttatgag gtaattactg aagagaaaaa agtatcattt ttttatgtag
    29881 atgagaaacc cttaaggtat ctgttatttg actatatatt tctactagtt ctagctaaaa
    29941 aaaaatatcc caatcttatg aactgcaccc caaaagttag atttattctg tctaactttt
    30001 ggggtgcagt tcattttgca tagttttggc attcatccta gatatccata taaaatgtct
    30061 gagattgttg aaatgtctaa aaaaatatca gatctaaacg ataaaaaaga atttcacaaa
    30121 aagaactgtg agttgaggtt tcaagatagt gttcttgata acataaatag tgtcccgggt
    30181 acaactcatt tatttagttg tttaaatatt caaacattag taaaaattat tttatatatt
    30241 tttcgataca acagttgtat gaaaatattt aattttagag ataaagagct tatctttttt
    30301 aataagattg taaatgggct gatacaaaat attgaggaga atttagagga tgatattgaa
    30361 aggatattga aatatttata tatatgttta tttaacgaaa tattcattat taagaataag
    30421 gtgaattttt ttgatgatgt ggaatttaat cagacactta gtgagttttt agataaattg
    30481 tagagggaat ggttatgagt aacaatacta agtatatctt tttatctcca catttagatg
    30541 acgcaatttt ttcctgtggt gattatattt caaaattaac ttcagaagga gaaattgtac
    30601 ttgttattac tatattttct ggttaccctt tgagccaaca gctacagccc tctgctaagc
    30661 aatttcataa gttgtgtaac cttgggaaat atccgattga agaaaggaag aaagaagaca
    30721 ggttagcttg tgaacgtcta caatgtgatt ttagacactt atcgtactat gaatgtctct
    30781 ataggaaaga tagaaatggc aattttctat accgtcatat ctatagtgaa ttaaaaaatg
    30841 aggacacatt aaagaatgat atcataaaag agctgttaat gcatttagac gacaaatgtg
    30901 tcgtatattg tccgctttca cttggtgacc atgttgctca tgtttttgtg aatagtattg
    30961 gaagggcgct tgaatttatg cgttataaag taatatatta tgaggacttc ccatatgtaa
    31021 gtgatagtag tatggttagt tatatgggaa aaactaaaga attaaaaatg tatcaagagg
    31081 aacttgatga aaaaaaatta tattgataga atatcatcaa tattgtgcta taaatctcag
    31141 attttgataa tctggaaatc agttgaaaaa cttttaaaca atattaaaga gttgtattta
    31201 aggaatgggg cagcttattc tattagattt tggattaaga aataaggaag tagttttatg
    31261 aatatagcag taattggttt ggggcatgtt gggctggcct acgcgttact atttgcatct
    31321 aaatataaag ttgttgcata tgatatagat tctgtaaaaa taaataattt aaaaaagggc
    31381 attcttccat ctaaaaatga agagcttatg aagttttttt gcgagaataa cttaaatatt
    31441 actttttttg atacattttc tgaaattaaa aataatattg attattatat tattgcgctt
    31501 ccgacagatt atgatgagaa aattggtagt tttaatacat atgaaatcga acaaacggta
    31561 tcgaagattc tgagggtaaa acctaatgga aagattattt taaagtcaac agttccgatc
    31621 ggcttttcaa acaaattaaa aaggctgttt gatacaaaaa atatcatttt tgtccctgaa
    31681 tttttgagag aaggttgttc tatatatgat aatttatatc caagtcgcat agttgttgga
    31741 gatgagacag ttgaaggaag aaaaattgca gagttgttcc tttcgattag tactcatagt
    31801 actgccaata ttaaaaatgt tatgttagtt tctcctactg aagcagaagc aattaagctt
    31861 ttttctaaca cattcttagc tctccgtgtt gcttttttaa tgaactagat tcttttgctg
    31921 agaggagaag cttaaatgct gaagttgtaa taaaaggtgt ttgtttagat ccaagaattg
    31981 gaaattttta taataatcct tcttttggat ttggaggata ttgtcttccc aaagatacta
    32041 aacaattaaa aaaagaattt atagaaataa atgccccagt gatagaagcg attgatattt
    32101 caaacacaaa tagaaaacag ttcatagtta aacaaatatt ggaacgaaag ccaaagatag
    32161 taggaatata taaattgggg atgaaatata attcagataa ttacaaagag tcagctattt
    32221 taagtataat taatgaacta ttgattgtcg gtataaaaat tttagtatat gaaccaaatt
    32281 taaacgttag tatagataac gtaattattg aaaagaattt tgaactattt acaaaacaaa
    32341 gtgatctaat tgttgcaaat agatgggata ggggccttga agcttataaa gataaagttt
    32401 acacaagggg gatctggata agagattaac tattgatatt aaaaaggcaa taatttataa
    32461 aatttgtaca aaaaaatgtt aaattttcac attttcatag tatactgttt aatagaaagt
    32521 aggttctttg taaccgctcc attataaaat cctttcactc ttccggtata ctattaggaa
    32581 aaacataact ggaggatatt ttgagctcac acctcatcgt tccagtggct gttccccaag
    32641 ctcgtgtact gcccaaaaaa gttagacatt ctatttagaa cgaggattga gttctgtatt
    32701 gtacaaggct cggtcctttt agagtcagct taaggctggc ttttttattt tttctaacta
    32761 tcagaatatt ttgacaaatg attgtaaata atgatataat atgcaaaagc taggaggtgg
    32821 tcagatgatt gagagaatgg aactggggga attttacaag gaattacgtt tggcgagaaa
    32881 gcttaagcag acagatgtgg cttgtgaggg tctaacagct tctcagttgt ccaagtttga
    32941 actagggcag tctatgctgt ctgcggataa gttgattcta gctatccaag gtataaatgt
    33001 gacctttgat gagtttgggc acaagctcaa caactatcaa gaatctccac atatgcgaat
    33061 cggtcgaaag gttgtgaatc gctttgccca tcaggatata gctgctttag agcagctatt
    33121 ggaggaagtc gatcaagaac agatggcgca gacctatcgt cgtttgaatg ccatcgtgat
    33181 taaagatgcc atccattcac taaataaaag ctacccacta gcagaggagg atagcgagtt
    33241 tttgaccacc tatctctatg ctatcgagtc ttggacctgg tttgaactct atctcttttg
    33301 caataccatg cccttcttga gcaatcaaga tctgattttt ttatcaacct ccttactcga
    33361 aaaatccaaa gaatttaaag agttagtaca caatcgattg tatatgaagc aaggactctt
    33421 aaatatctta tcagaactca tggagcgaaa acttttctcc tacatcccaa tcttcgaagc
    33481 cgagttggag aggatgttac gaccgtatga tgtttttgaa aaagtatcgt ggcaattttt
    33541 aaagaagatg agtgtctttc ttcaaacaaa gggaagcaat caaaaagaga ttgaacgctt
    33601 tatccaatct ctgcaggtat tagaaaatcc acaattaata gccctttttg aattgcgttt
    33661 tcagcaatat aaagaactta tagattagtt agaaatggca aaataataat aatctcagca
    33721 acgattttca ataatgttaa aatttgtttc ttcatatttc tccactcctc attagtagta
    33781 actttattat accagagaag taatcaaatt caccaaaatc gcaaatctga aatttctata
    33841 agaaaaaata tcaaatatgc gattttctaa aataagccaa tgttcgtgtt atactgtact
    33901 tgtaaagcac ttgaagcaaa tcctaggtcg cagaagtggt ttacaaatga agataattga
    33961 aggagtgtaa aagatgttaa atttacaatt tgcagaaaca atggaattga cagaagctga
    34021 gttgcaagat gttagaggag gcaacctcgt aaattctatg ggaggtggtg gaagaagtgg
    34081 tatatctggt tggggcgtcc caggaattta ccctggttgg ggcaatcaag ggatgagtcc
    34141 taatcgtgga gcttttgatt ggactataga tttagcagat ggattgtttg gccgccgtag
    34201 aagataggct cttataggag ataataggat gaagaaaatg aaagaagtga aatttcacct
    34261 agcaactggc ttgttgattt tgacttatta cctcattttt aatgtcacat cagacttaga
    34321 ttttatggtt gctttgtctg ataatatgta ctatgtgttc caagttttat tggtacttat
    34381 tctgggaact atagctacaa ttgcttttgt caaaagtgaa cattggaaag agtgtggtcg
    34441 tttccagttt cgttggtctt acctgggtgt tttccttctt tcttttttct tattgttcgt
    34501 gtgggcaaat ctaacaacct atatctttcc tcgtacccag aatggaagta cagtcgtaga
    34561 agtagccacc aatttaacag gaattagcta ttttgttacg cgaatacttt atactagtat
    34621 tatcgcacct gtatcagaag aggttgtttg tcgtggtctt ttgatgacaa gtttatctaa
    34681 agttaagaga tactatcttg atgttcttgt ttccgctgcc atatttggcg ctatgcatgt
    34741 tttgcagtat ggttggataa cgacagattt cataaagtat tttggaatgg gcttgatatt
    34801 ttgtatgatg tttcgctaca cccgatccat ctattgggct atagctctgc atgcttcatg
    34861 gaatagtttc ttactcattg ttactttatt ggtatttgga tattagaaag gtgatatatg
    34921 aaaaagataa tctcacaccg ctactttatt atagtttttc tactagttat tgctgaccag
    34981 aagttcagtg ttctagtttt acgcagcgac cttgttactg ggctatctga ctttgcctat
    35041 tatctgtcgg atatgatgtt gaattttctt gtggttttat ttgctcttat tgctatgatt
    35101 tggtcgggga aatggcagaa aatcaatagt agaaagttta aaggatccta tcttttctat
    35161 tccttcctag ctctgcttgc ttttgttgtt tggaatttcg ttaccttttt tcttttccca
    35221 cctactcgaa atgaaatttc ttatcaacat gctgctccga cttttacagg ggctacagca
    35281 tttttgatgt atttttttta tcctgtgatt gcaggtccca tttttgaaga catgatttat
    35341 cgtggattag tgatgactgc tctggaaaaa ggaaagaaat gggggctaga tgtacttggt
    35401 tccgctgttt tatttggggt ctcgcacatt agtaatcacg gttgggtctt gacagacttt
    35461 gtcttctata tgggtggagg tcttatattt gcagtcttat ttagaatgac aaagtcaatt
    35521 tattggccta ttggactgca tatagtctac aatggcattg gtcagctttt gatgttactg
    35581 taattttggt tgttagtagt atcgcggtct atccttgggg aaagattccc atttgaactt
    35641 aagaaggagt tgtgatgaaa gtatttcttc aaaatagaga ttttaggcaa ttaactatca
    35701 accagtggat ttcaacgctt ggggatacga ttttttatct ggccttcctg aattatgtgg
    35761 cagatgcatc ttttgcccct ttggcgattt tactcatcac gatttcagaa accctgcccc
    35821 aagttctaca aatctttctg ggagttttgg cggattttca acatcatcgt gtcttgaaat
    35881 acacagtcat tagttttgca aaatttttgc tttactctat agtgtcttta tcactttcag
    35941 ggcagtcttt ctccttgttg ctagtagcat ttatttgtct gattaacctc ttgtctgaca
    36001 cattgagtta tttttcaggc gccatgctca ctccgatttt cattagaatt attgggcaag
    36061 accatctggc agaagctatt ggctttaaac agtcaactgt tagtttagtg aaaacaatca
    36121 gtaatatcct aggaggagtc ttactaggca ttctatccat ccagtttatt tccttactga
    36181 acgctcttac ctttttaatc gcatttttag gtatcctctt cataaaaact gacctcttga
    36241 aagtagaaaa aacgattagc tatcaagaag gactctctgt aaaatccttt tgccagcatt
    36301 tgctccaatc atcgaaattg atatggaata tgaataaggt gctcttggtt ttgtttatta
    36361 tctctactag tcaagcagtg ataaatgtta cggttcctat ttccactcta tttttaagga
    36421 accagccctt tttgaattta caaactggtc aatctcttgc cttgttagcc acactagaat
    36481 tatcagccct gattgtcgga agccttgtaa gtggctatct gcaacatacc atttccataa
    36541 aaacagctct ctatgcctca cttgtcatcc agttgcttct tctagtagga tttgccacag
    36601 ttcgttttga ctggattctc atctttagta ccttggatgc ctttttcgca ggtgttctct
    36661 ctcctagatt gcaagaactc gtttttaaac aaatacctga ggagtcaatg ggagcagttc
    36721 aatcctctat cggtgccatt acggttgttt tacctagctt atttacaata gctttggtaa
    36781 ccattgctac tagctttgga actctggcag ttagctttgt tttattgcta tttcttctag
    36841 ttgcctttgt tatgcttctg aatattcgtg aaagtattta gtggagaatt tctataatat
    36901 atgtttgaga ctggattgcc agtcttttta tgatggtcaa aatcaacaga cttttttctc
    36961 ttttctgaat gtgttata
//
LOCUS       DAVDWM010000015        35998 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVDWM010000015 DAVDWM010000000
VERSION     DAVDWM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.015"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(613..1026)
                     /gene="ndk"
                     /locus_tag="VUW49_000950"
     CDS             complement(613..1026)
                     /gene="ndk"
                     /locus_tag="VUW49_000950"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7980884.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(1137..4814)
                     /gene="rpoC"
                     /locus_tag="VUW49_000951"
     CDS             complement(1137..4814)
                     /gene="rpoC"
                     /locus_tag="VUW49_000951"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET7980885.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNVNLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4847..8458)
                     /gene="rpoB"
                     /locus_tag="VUW49_000952"
     CDS             complement(4847..8458)
                     /gene="rpoB"
                     /locus_tag="VUW49_000952"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7980886.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8874..8944
                     /locus_tag="VUW49_000953"
     tRNA            8874..8944
                     /locus_tag="VUW49_000953"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8906..8908,aa:Cys,seq:gca)
     gene            complement(9142..10473)
                     /locus_tag="VUW49_000954"
     CDS             complement(9142..10473)
                     /locus_tag="VUW49_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET7980887.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10764..11588)
                     /gene="endA"
                     /locus_tag="VUW49_000955"
     CDS             complement(10764..11588)
                     /gene="endA"
                     /locus_tag="VUW49_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET7980888.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11627..11815)
                     /locus_tag="VUW49_000956"
     CDS             complement(11627..11815)
                     /locus_tag="VUW49_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7980889.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11808..13091)
                     /gene="murA"
                     /locus_tag="VUW49_000957"
     CDS             complement(11808..13091)
                     /gene="murA"
                     /locus_tag="VUW49_000957"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7980890.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(13115..13345)
                     /locus_tag="VUW49_000958"
     CDS             complement(13115..13345)
                     /locus_tag="VUW49_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET7980891.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(13403..14440)
                     /locus_tag="VUW49_000959"
     CDS             complement(13403..14440)
                     /locus_tag="VUW49_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7980892.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(14424..14912)
                     /gene="coaD"
                     /locus_tag="VUW49_000960"
     CDS             complement(14424..14912)
                     /gene="coaD"
                     /locus_tag="VUW49_000960"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7980893.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14902..15441)
                     /gene="rsmD"
                     /locus_tag="VUW49_000961"
     CDS             complement(14902..15441)
                     /gene="rsmD"
                     /locus_tag="VUW49_000961"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET7980894.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQITKEIGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(15506..16498)
                     /locus_tag="VUW49_000962"
     CDS             complement(15506..16498)
                     /locus_tag="VUW49_000962"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET7980895.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16609..17292)
                     /locus_tag="VUW49_000963"
     CDS             complement(16609..17292)
                     /locus_tag="VUW49_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET7980896.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(17348..18088)
                     /locus_tag="VUW49_000964"
     CDS             complement(17348..18088)
                     /locus_tag="VUW49_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7980897.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQREEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18122..18400
                     /locus_tag="VUW49_000965"
     CDS             18122..18400
                     /locus_tag="VUW49_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7980898.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            18478..19404
                     /gene="yidC"
                     /locus_tag="VUW49_000966"
     CDS             18478..19404
                     /gene="yidC"
                     /locus_tag="VUW49_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7980899.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(19479..20273)
                     /gene="pflA"
                     /locus_tag="VUW49_000967"
     CDS             complement(19479..20273)
                     /gene="pflA"
                     /locus_tag="VUW49_000967"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET7980900.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(20399..21649)
                     /locus_tag="VUW49_000968"
     CDS             complement(20399..21649)
                     /locus_tag="VUW49_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7980901.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21716..22543)
                     /gene="purR"
                     /locus_tag="VUW49_000969"
     CDS             complement(21716..22543)
                     /gene="purR"
                     /locus_tag="VUW49_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET7980902.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22810..23751)
                     /locus_tag="VUW49_000970"
     CDS             complement(22810..23751)
                     /locus_tag="VUW49_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET7980903.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(23741..24997)
                     /locus_tag="VUW49_000971"
     CDS             complement(23741..24997)
                     /locus_tag="VUW49_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET7980904.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24999..25661)
                     /locus_tag="VUW49_000972"
     CDS             complement(24999..25661)
                     /locus_tag="VUW49_000972"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET7980905.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25624..26280)
                     /locus_tag="VUW49_000973"
     CDS             complement(25624..26280)
                     /locus_tag="VUW49_000973"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7980906.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(26291..27169)
                     /gene="rsgA"
                     /locus_tag="VUW49_000974"
     CDS             complement(26291..27169)
                     /gene="rsgA"
                     /locus_tag="VUW49_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7980907.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(27171..28043)
                     /gene="rsmA"
                     /locus_tag="VUW49_000975"
     CDS             complement(27171..28043)
                     /gene="rsmA"
                     /locus_tag="VUW49_000975"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7980908.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILVDTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(28429..28575)
                     /locus_tag="VUW49_000976"
     CDS             complement(28429..28575)
                     /locus_tag="VUW49_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980909.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDKNMTNHLLLVQILVHGYLLDFA
                     SIEG"
     gene            complement(28568..29209)
                     /locus_tag="VUW49_000977"
     CDS             complement(28568..29209)
                     /locus_tag="VUW49_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980910.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(29211..31241)
                     /locus_tag="VUW49_000978"
     CDS             complement(29211..31241)
                     /locus_tag="VUW49_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET7980911.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWNQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(31318..31614)
                     /locus_tag="VUW49_000979"
     CDS             complement(31318..31614)
                     /locus_tag="VUW49_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET7980912.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(31934..32833)
                     /locus_tag="VUW49_000980"
     CDS             complement(31934..32833)
                     /locus_tag="VUW49_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7980913.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEECLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(32905..33465)
                     /gene="rnmV"
                     /locus_tag="VUW49_000981"
     CDS             complement(32905..33465)
                     /gene="rnmV"
                     /locus_tag="VUW49_000981"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET7980914.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(33465..34238)
                     /locus_tag="VUW49_000982"
     CDS             complement(33465..34238)
                     /locus_tag="VUW49_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7980915.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQKQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAAATTANAERIFGLDSK"
     gene            complement(34543..35436)
                     /locus_tag="VUW49_000983"
     CDS             complement(34543..35436)
                     /locus_tag="VUW49_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET7980916.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSTPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(35720..35854)
                     /gene="rpmH"
                     /locus_tag="VUW49_000984"
     CDS             complement(35720..35854)
                     /gene="rpmH"
                     /locus_tag="VUW49_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7980917.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT        10103 a         8394 c         6904 g        10597 t
ORIGIN      
        1 ttattccatg ttattttgag gaagatattg tgaaagtcaa agatgatgat aaagttgagt
       61 ggaatttgtt agaatttcaa aaatttagag catttttgac ttagtaatct gtgttgaagg
      121 ctcaaaacct atggtaaaaa agtaactttg aaaacgtatt gcctccaaag atttagttaa
      181 ataatgattt aacacaaaaa gaaattattg aagttctgga aagatgttgt ttcagtattg
      241 agaaaaggtg ggaaaaactt gcgattttca cagagaaagg aagaaaaagt atagaaatat
      301 agtcaattga aacaagaaca ggataaaaga accttttgtg ccatattttt ctcctttcgc
      361 tttacaattg gattgaacac ctttattgta tcgcgtttgg agtttttttg gtataacctt
      421 cgacgcgcac ccgcatagcg ggtgtttttt tgtctcgcac ctaacggagc gagacgaact
      481 aatagtcact taatcaaaaa acgcaccata tcaaaaacta aaaagtttga tatcatgcgt
      541 catgtcttaa actaattgac tatactttct attcaaatga gcttttaacc aattgattga
      601 gccaatccac tcttaaaacc aaagagcaat ttctcgctta gctgactctt ctgaatctga
      661 accatgtaca acattttgga taacctcatt ttctccagca gcttttgcaa agtcacctcg
      721 aatagtgcct ggtaaagctt cttctggacg agttgcaccc atcatggtcc gccaagtttc
      781 gattactttg ggaccagaaa tgacacccac aagaactgga cctgaagtca tgaattcacg
      841 aatcggtggg taaaaactct gaccaaccaa gtcctgatag tgctggtcaa tcaactcttc
      901 tgaaacctgt gaacgaaact ccaatttttc gattgtaaat ccacgttgtt cgatgcgctt
      961 taacacttca cccactagcc ctctttttac accatctggt ttgatgataa agaatgtttg
     1021 ttccataccc gtctcctttg tcagcttctt tcttttattt taccacattt cgtggaaaaa
     1081 tggagaaagt tttcagaaga gagaatgaga gaacccgagg gttctctcat tctctcttat
     1141 tctactgttt cttccacagt ttcaacggca gtatccacaa ctacttctgt tgtttcttca
     1201 tttccttctt cctctactgg aggattaagg tattcttctt cgttgacagc atgtggttca
     1261 aggttacggt aacgggccat accagtacca gctgggatga tcttaccgat gataacattt
     1321 tctttaagtc caaggagatg gtctttctta ccacggatag ctgcgtcagt aaggacacga
     1381 gttgtttcct ggaaggaagc cgctgacaag aaactgtttg tttcaagtga ggctttggta
     1441 attcccataa ggactgggcg acctgtcgct ggaactccac ctgcgataag gacatctttg
     1501 ttggcatctg taaagtcatt gatatccatg agggtaccca tgagaagatc tgtatcacct
     1561 ggatccatga cacggacttt acggatcatt tgacgaacca ttacctcgat gtgtttgtca
     1621 ccgatttcta ccccttggct acggtaaact ttttgtactt caccgagaag gtacgtttca
     1681 actgacaaga catcacgaac tgcaaggaga cgttttggtt ggatagaacc ttctgtcaga
     1741 gcagcaccac gcgctacttg gcccccaact tcgacacgca tacgagctgt aaatggaacg
     1801 acatattcac cttcgccagt ttcaccctta acaaagactt tcttggtacg agttgatgca
     1861 tcctcttcga tagcagtaac ttgtccttta acctctgtga taaccgcttc ccctttagga
     1921 ttgcgggctt caaagatttc ttggacacga ggaagaccct gagtgatatc ggtatttgag
     1981 gcaaccccac ctgtgtggaa ggtacgcatt gtaagctgtg taccaggttc cccgatagat
     2041 tgggcagcga ttgtaccaac tgcttcacca acttcaaccg catcaccagt cgccaagttg
     2101 ataccgtaac agtgacggca gacaccgtga cgagtgttac atgtaaatac agaacggata
     2161 gtcacttctt ccacaccagc attgacaatt tcacgcgcct tgtcttctgt aatcaattca
     2221 tttggaccaa taatcactgc accagtttct ggatgtttaa cagttttctt agtgtaacga
     2281 ccgttgagac gctcttcgag agactcgatc atctcttttc cttctgcgat agaacggatc
     2341 aagagaccac ggtcagttcc acagtcgtcc tcacggatga taacgtcttg ggcaacgtcg
     2401 accaaacgac gagtcaagta acctgagtcg gctgtcttaa gggccgtatc ggtcatacct
     2461 ttacgagcac cgtgagttga gaagaacatt tccaataccg acaaaccttc gcggaagttt
     2521 gaaaggattg gcaattccat gatacgtcca ttcggagcag ccatcagacc acgcataccg
     2581 gcaagctgtg agaagtttga gatgttacca cgggctccag agtccatcat cataacgatt
     2641 gggttcttag gatcttgatt agcaatcaag cgtttctcaa gtttttcacg ggcagcacgc
     2701 cattcagctg taacagcatt gtaacgctcg tcgtctgtga tcataccacg acggaattgt
     2761 ttggtgattt gttcgacacg tttgtgtgat tcttcaatga tttcagcctt gtcatcaacg
     2821 actgggatat cggcaatacc cactgtcaat cctgcaagag ttgagtggtg gtaaccgagg
     2881 ttcttcatgc ggtcaagtag ggcagaagtt tctgtcgtac ggaaacgttt gaagatttca
     2941 gcgatgatat ttccgaggtt tttcttcttg aatggagggt tgagctcaag attgctgata
     3001 gcttccttga tatctccacc aagtggcaag aagtatttag ctggaacacc ttctgtcaag
     3061 ttgacattgt ttggttcttg caagtatggt agcccctctg gcatgatatc gttgaagaga
     3121 attttaccaa ctgttgtaag caagacctta tgtctttgct cttctgtcca aggcttgttg
     3181 aggctgtctg ttgcgatacc aacacgtgag tggaggtgaa cataaccatt gcggtaagcc
     3241 ataaccgctt cgtcacggtc tttgaagacc attccttcac cttcgcgacc agcttcttcc
     3301 atggtcaagt agtagttacc caaaaccatg tcctgagatg gagtaactac cggtttccca
     3361 tctttcgggt tcaagatgtg ctcagcagct agcatgagga tacgagcttc tgcttgtgct
     3421 tcttctgaaa gtggtacgtg gatggccatt tggtccccgt caaagtcagc attgtaggct
     3481 tcacagacaa gtgggtgcaa gcgaagagcc ttaccatcaa tcaagactgg ctcgaaggct
     3541 tggataccca aacggtgaag ggtcggtgcg cggttcaaaa gcactgggtg ttctttaatc
     3601 acttcttcaa ggatatccca gatacgctca tctccgcgtt ccaccaagcg tttagctgct
     3661 ttgacgtttt gcacgatatc acgggcaacg atttcacgca tgacaaatgg tttaaagagt
     3721 tcaatcgcca tttcacgtgg aacaccacat tggtacatct taagagttgg accaacggcg
     3781 ataacggaac gtcctgagaa gtcaacacgt ttaccgagca agttttgacg gaagcgtccc
     3841 tgtttacctt taagcatgtg gctcaatgat ttcaatggac ggctacctgg tcctgtgatt
     3901 ggacgaccac gacgaccatt gtcaatcaaa gcgtcaactg cttcttgaag catacgcttc
     3961 tcattttgaa cgatgatacc tggtgcattt aactcaagca aacgagccaa acggttgtta
     4021 cggttgataa cacggcggta aaggtcattc aagtcagatg aggcaaaacg gccaccatcc
     4081 aactgcaaca ttggacgaag atctggtggg ataaccggaa ggatgttaag aatcatccat
     4141 tcaggtttgt ttccagactt gtaaaaggca tccaaaacat ccaaacgacg gatggctttg
     4201 acacgttttt gtccagtagc tgttttcaat tcttctttga gttcagcaat ttctttttca
     4261 agatctactt gcttcaaaag gtcttggatg gcttccgcac ccatcttggc aacaaatgaa
     4321 ccataaccat attcacgcaa gcgctctcgg tattcgcgct ctgtcatgat agacttgtgc
     4381 tcaagtggtg tatccttagg atcaatcacc acataagccg caaagtagat aacttcctcg
     4441 agggcacgag ggctcatatc aagggtcaag cccatacggc ttggaatccc cttgaagtac
     4501 cagatgtgag atacaggagc tttcaattcg atatgtccca tacgctcacg acgaactttc
     4561 gtacgcgtta cttcaacccc acagcggtca caaacaattc ctctgtaacg aatgcgtttg
     4621 tacttaccac aagcacattc ccagtctttt gtaggaccaa agatcacttc atcaaagagt
     4681 ccttcacgtt ctggtttcaa ggtccgataa ttgattgttt caggtttttt gacttctcca
     4741 taagaccatg aacggacttt acttggagaa gctagggtga tttgcatact tttaaaacga
     4801 tttacatcaa ccactatttc ttccctttct attctaagtg aactgcttat tcttgttcag
     4861 cagcttcttc tgttgcttcc gcttttgttg ctttctcagc ttcttcagct tcaaaggctg
     4921 ctttagcctc ttgggctgct ttttcgcggg ctttttcaag gtcatctacg tggatgacat
     4981 cttcgtccat tccttcatcc aagtcgcgaa gttccacttc ttggtcatct tcgtctagga
     5041 cacgcatgtc aagaccaaga gattgcaatt ctttgacaag aactcggaag gattctggaa
     5101 cacctggttt tggaattggt ttgccttttg taatagcttc ataggctttc aaacgtccgt
     5161 tgatatcgtc cgacttgtaa gtcaagattt cttgaaggac atttgacgca ccgtaggctt
     5221 caagagccca aacctccatc tcaccgaaac gttgtccacc aaactgagct ttacctccga
     5281 gtggttgttg ggtaacagtt gagtaaggtc cgactgaacg cgcatgcaat ttatcgtcaa
     5341 ccatgtggtg gagtttaatc atgtacatga ctccaacaga aacacggtta tcaaatggtt
     5401 cacctgtacg tccatcgtaa aggattgtct tggcatcgct atccatacct gcttctttaa
     5461 cagttgacca aagatcttca gagcttgctc catcaaagac tggtgtcgca atgtgaatac
     5521 caagagtacg agctgccata ccaaggtgaa gctccataac ctgaccgata ttcatacgtg
     5581 atggcacccc aagtgggttc aacatgatgt cgactggagt tccgtctgga aggtaaggca
     5641 tgtcttctac aggaacgata cgagagacaa cccctttgtt tccgtgacgt ccggccattt
     5701 tatctccgac cttaatctta cgtttttgag cgatgtaaac acgaaccaac atgttaacac
     5761 ctgattgcaa ctcatctcca tttacacgtg taaagatctt aacatcacga acgacaccat
     5821 cggcaccgtg tggtacacga agagaagtat cacgcacttc acgagacttg tctccaaaga
     5881 tagcgtgcaa gagacgttct tcagctgaaa gatctttctc acccttaggt gttactttac
     5941 ctacaagaat atcaccttct ttaacctcag caccaatacg gataatcccc atttcgtcaa
     6001 ggtctttgag ggcatcttca ccaacgtttg gaatttcgcg agtgatttct tcaggcccaa
     6061 gctttgtatc gcgcgtttct gattcgtatt cttcaaggtg aacagatgtg tagacatcgt
     6121 ccttcaccaa gcgttcgctc atgataacgg catcctcgaa gttgtaacct tcccaagtca
     6181 tgtaggcaac gattgggttt tgtccaagcg ccatttctcc attttccata gaaggtccgt
     6241 cagcgatgaa atcgcctttt tcaacgacat caccaacttt tacgagagtg cgttggttgt
     6301 aagcagtacc tgagtttgaa cgacggaatt tttggatgtg gtaaacatcc aatgaaccat
     6361 cttcacgacg aacttctacc ttgtcagcat ctgcgtaagt aactttacca tcatactgag
     6421 caatcacagc cgcaccagaa tcgtgggctg cttggtattc cataccagta ccaacgtaag
     6481 gtgcctgagg attaatcaat ggcacagcct gacgttgcat attggctccc atgagggcac
     6541 ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacctgtt
     6601 ttggtgaaac gtccatgtag tcaacaatat tagctggata ctcttggttg accccttggt
     6661 gacgtcccat gacaatcttc tcagcaaagg ttccatcttc attcagacga gagttagcct
     6721 gagctacagt atattcatct tcttcatcag ctgtcaacca aacaatttcg ttcgtgacaa
     6781 cacctgtttc acggtcaacc ttacggtatg gtgtttgaac aaaaccatat ttgttcaagt
     6841 gtccgtaaga tgacaagtta ttgatcaaac cgatgttagg tccttcaggt gtctcgattg
     6901 gacacatacg accatagtga gtgtagtgca cgtcacgtac ttcatatccg gcacggtcac
     6961 gagtcaaacc accaggtcct aaggctgaca aacggcgttt gtgagacaac tcagaaagcg
     7021 ggttgtgttg gtccatgaac tgtgacaact gtgatgaacc aaagaattct ttaactgcag
     7081 ctgttacagg acggatattg ataatttgtt gtggtgtcaa gacttcattg tcctgaacag
     7141 acatacgttc acggacatta cgttccatac gagaaagtcc caaacgtact tggttggcaa
     7201 gcaattcacc aaccgcacgg atacgacgat ttccaaggtg gtcgatatca tctacacggc
     7261 caagtccttc agccaagttg aggaagtagc tcatctcagc aaggatatct gcaggagtca
     7321 ccgtacgaac cttgtcatct gggttagcat taccaatgat cgttacgacg cgatctggat
     7381 cagttggagc aataaccttg aatttttgaa gaacaacagg ctcagtcaca acggctgcat
     7441 cgtttgggat gtagacaatc ttgttcaagt cgccatccaa atggctttca atgctttcaa
     7501 tcacgctacg agtcataatc gtaccagctt ctaccaagat ttctccagtt tcagggtcta
     7561 ccaatggctc tgcaatggtt tggttgagca aacgtgtttt aacattgagt tttttattga
     7621 ttttgtaacg accaactgct gccaagtcat aacgacgtgg gtcaaagaag cgagctacaa
     7681 gcaagctacg tgagctttca gccgtcttag gctcacctgg acgaaggcgt tcgtaaattt
     7741 ctttcaaggc ttcgtctgta cgagagtcca ttggattctt gtggatatct ttttcaacag
     7801 tgttgcgaac caattcgctg tctccaaaga tatcaaagat ttcatcatca cctgagaaac
     7861 caagagcacg aaccaaggtt gtaaatggaa tcttacgagt acggtcgata cgagtgtagg
     7921 tgatatcttt tgagtcgctt tcaagttcca accaagctcc acggttaggg ataacagttg
     7981 aaccatagcc caccttacca tttttgtcta ctttgtcgtt aaagtaaaca cctggtgagc
     8041 ggaccaactg agaaacgata atacgttcac caccattgat gatgaaagta cccatttctg
     8101 tcatgattgg gaaatcacca aagaaaactt cttgggtctt gatttcgcct gtttctttat
     8161 tgatcaaacg gaaggttaca aaaattggtg ctgagtagct agcatcgtgg atacgagctt
     8221 cttctagcgt gtattttggt tccttgattt catatccaac aaattccaac tccattgtgt
     8281 ctgtgaagtt tgaaattggc aatacatctt caaacacttc cttaagaccg tggtctagga
     8341 aagctttgaa tgagtcagtt tgaatttcaa tcaaatttgg taagtcaaga acttctttga
     8401 ttcttgaaaa actacgacgg gtacgatgtt tcccgtattg aacgtcatgt cctgccaaga
     8461 tgattctcct ttgtaaataa gttccaagcc ttgtcaatca ggcttttcta atcgtcatat
     8521 ggttgtaaac cccttatcac cgtgtcctct tgacgaattt tcagaatctt taagcctctg
     8581 ttacaaatgc tcataatctt gaaaaaaagc acaaaaagag cagctaaatc tgactttttc
     8641 agaagattta actgctgtga gccttgtctg gacaatattt cagacaaaac ctacgacaaa
     8701 tgattaccca tattataccc tatttagcta gatttttcaa ggggtttcag taggtttttg
     8761 gtaaattttt tcccatagaa aacttggcat cacattcgaa tcacgctatg gtacaaaaaa
     8821 ctgaaaaaac tattgactga aaatcatttt caaggtataa taataaacgt taaggcggta
     8881 tagccaagtg gtaaggcacg gctctgcaaa agcttgatcg tcggttcaaa tccgtctacc
     8941 gccttctata acttgattta tcaggtttca aatgaacaga aagcccaatt tgaagggctt
     9001 tttttatttt ccctcgaata aatacgtata actttaaaaa cttttggagc gagtttgtgg
     9061 cagagttctt tccatggcat aattcccttt tgaaatcaat tgaaaaaagc ttcgtcacgc
     9121 ctggtgacaa aagcctctag tttattctgt ttcttcttct atttcgactt ctgtgatttg
     9181 gacttttacc ttggtaacac gtccattctt cactttatca ttggttaaga taaactgttt
     9241 gttttggctg accaattcat agctgagttt ctcagtcgtt ggaatggttc ccactcctgt
     9301 caaataataa ccagcgatgg tatcaacgtc atcactttcc agctcaacat caaagtaatt
     9361 attgaaatca ttgagggtca tagttccttg aacaatgtag gtatcctcgc caatctgatg
     9421 aacatctatt gctgctttgt ccgtttcatc atcaatttca ccaacaattt cctctaagag
     9481 gtcttccaaa gtcactaaac cagccatacc accgtattca tccagcaaga ttgccatttg
     9541 tctttgggta tttcgcagtt cttttagcaa gtcatccaca aaaatagttt caggtacaaa
     9601 aagtggatct tgtaaaattc tcttccaaac aatattgtca aaaccgtcca caaagcctgc
     9661 cttaaggaga ctcttggtgt gaatgattcc aattacattg tccttatccc catcataaac
     9721 cgggatacga gaataatttt gttttaaaat actttggata atggcttgac tatcatcctg
     9781 aatatccacc ataaaggcat ccgttcgagg aaccataacc tctcgtgcca tcagttcatc
     9841 gagcgaaaag acaccttgta gcatctcaat ctcatcagca tccaatgttt cttcactatt
     9901 tgtcagcata taggcaattt catcacgggt cattttttca tccgcatcat cgaatgacat
     9961 aggagtcaaa tggctcaaga aattggtcga agcagctaaa agccaaacaa aaggactgac
    10021 tagttttccg atcccaatga taaccggcgc tgtacgaatt gccaaggcat cctttagatt
    10081 aagagcgatt ctcttaggat ataattcccc aaaaacgatg gaaatatagg tcaaaaatgc
    10141 caaggataga aaagttgcca cggcttgtgc tgtttcgcca ttcccaagcc aagaggcaat
    10201 cacacgtcct agagtatcag ttaaactcgc ccctgataag attgtaatca gggtgattcc
    10261 tacctgaatg gttgataaaa agtggttagg attttctagt accttcagca ggcggatgta
    10321 gcgtctgtct ccttcttccg ccttttgttc aactcgggca cgattaagag aaaccatggc
    10381 catttctgtg gctgagaaaa aggcatttaa taccgtcaag ataaacaata aaacaaattg
    10441 tagcaacaaa ttctgactac ttgggtcttc catagttctt ctcctaaaat agtatcttat
    10501 cctttattat atcacaaaat ttaatccaat acatattatt tttttcttag cgtaacttcc
    10561 atctctctcc caaaactgga agttagtcta agacggattt ccactgattt cacgcgtttt
    10621 tctcatacta aaagctaggt tgtgggaaac tggaagttag ttttagaagt tactattttt
    10681 tcttagtacc tattctagcc ctgcttaaac tagtaaaaag aactcctaca tccataagtg
    10741 acataggagt ttaggcttat cttttactga gttacagtta cttctccagt tcggtaatcc
    10801 agttgaagtc ccttttgaac attgggaact agaacattga attccaattc tccatccgaa
    10861 gacttggctt caatctgtga agctgaggga actaaatcct cgtttgaagc gtagtaaagg
    10921 gttacacggt aacggacacg cttgttttgg tccaaggctt tacgcacctt gctttcatag
    10981 tagttttgac cagtcgaata ctcggcttgt gcctgatttg cccaggctgt ctgaacagca
    11041 atgtttttag gattgcttgt tgaggcatca aaaccatcca aaccaccgat taaggcatag
    11101 cctaacaaat gacctctatc gactgcatgg gtataagagc cctttagatt cttgacctga
    11161 tgccaacctg gaggagtcca agaagttgaa ccattcccag tttctttacg attcttgtac
    11221 tgacgagtgg ccttagacaa gagggcatta gctacggttg gaacagtttc cttgcccact
    11281 gtctttgttt tattgtcagc gtagggctta cttgaaacct tggcatctag atttgtttta
    11341 ttaccattga cgataaaagc acctgagcca ttccactcca gactcccctt tatttgactc
    11401 ttgactgcgt ctgttaagac actctctgcc aatgcttgac taggagcttc agacgcttgt
    11461 tttttctgac taagattggt tttgggacta ttaggtgccg actgcatctg cttgatataa
    11521 tagctccctg tagacaaaag caataacact agcagtccga ttagtgtctg tcttgttttt
    11581 ttgttcatat ttctccttat ctttagaaaa aggctggttc tccagcctaa tttcctgtaa
    11641 atttatgaat caattcctgc cattttgctg gagacaggat agcccatgga tcttgaccct
    11701 tgcccaagat tccataacct accattagac cgattcctag agccagagta cctaaaatca
    11761 gtactatgat gactaaaagt aaacgattga ttacgtagct tgatttctta ttcatcttca
    11821 tcgcttgcct caatccgctg aatcttagca cctagctgcg ccaacttctc atggaaaccg
    11881 tagtaacctc tatccaagtg aaccaattta ccgaccacag tttctccctg tgctaccaaa
    11941 cctgtcaaaa tcaaggctgc actggcacga aggtcagttg aaagaacttc tgctccctgc
    12001 aaaggctgtc caccaacaat acgagctgta tcacggataa tctcagaatg caagcccatg
    12061 cggcgcatct cttctaggtg ttggaaacga ttttcgaaaa ctgtctccac catggttgat
    12121 tcgccttttg caactgtcat caaggctgta aattgagcct gcatatctgt tggaaatcct
    12181 gggtggggca aggttttcac atgaacagct tttagatttt ctagttgaga acgaacacga
    12241 attccttcgt cttcttcaat tacttcaaca cccatttcaa gtaacttggc aatcaaggga
    12301 cggttgtgct cccagacagc gtctcgaatc aagacatcac caccagtcat ggcagcagct
    12361 accataaagg ttcctgcttc gatacggtct tggactacat tgtgagtcgt accatgaagt
    12421 ttctcaacac cagtaatggt tatagtctct gtaccagcac ctttgacctt ggctcccatt
    12481 tcattaagga gaatggctaa gtcaacaatc tcaggctcac gcgcagcatt ctcaatcact
    12541 gtcaccccat cagccagagt cgctgccatc atcaagttct gcgttgcacc aacacttgga
    12601 aagtccatat agatatgagc accatgcaag cgttctgcct tggcttcgat gtaaccagct
    12661 gtctgactaa tcttaacccc catagcttcc agacctttca aatgaagatc aataggacgg
    12721 ctaccaatcg tacaaccacc tggcatggat accttggcat gacccacacg ggcaaggatt
    12781 ggccctaata caacgatgga ggcgcgcatc ttgctgacat acttgtaagg ggcttcctca
    12841 gtgatgtcgc cagtagcatc caccttgaca agatgagctt cctcatcaaa gtcaaccttg
    12901 gcattcaaac caccaactac ctgattcata ataaagacat ccgacaaaat cggaacattc
    12961 tgcaagacgg tctttccttc acttgctaga atagtcgctg ccaacaaggg taagactgca
    13021 ttttttgctc cctcgatcgt cacgcttcct accagacgat tatcgccacc ttgaaccaca
    13081 attttatcca tactcgtttc ctttactctt gatttcataa taatcctcta agcgcttctt
    13141 gccacaaatg atacaggcta ataaagaaag aactcaggat gtagcctatg acaatgctaa
    13201 aaaaggctac taataaacga acttttcttg tattctcatc tgtcactttt aaaaccttct
    13261 cccatctaac aagatccttt aaaaggtaaa aactcacata aataaagagc atatgactac
    13321 ttagagtgaa taataattga accatttgac tattatacca tcttctctgt ttgtgcgcta
    13381 gtttggaaac ttcactcaaa aattagggat tgtttttcaa gtaatcaatc gcatcttgta
    13441 gggttttaac gggcacgatt ttcatatccg tcttgattgt tttagccgct tctagggctg
    13501 tttggtagtt gtttttcgcg tccggatgcg ccttttgttc ttcttcgcta acagggttat
    13561 caggagcaaa gaaaatagca gcacctgccc tagccgaagc tacaaccttc ttatcaatac
    13621 ctccaatgtc tcccacatta ccatcgcggt caatggtacc tgtaccggca acaatacgac
    13681 cattacgaag atctgggtga gctatttgag tatagatagc tagactaaac atgagaccag
    13741 cacttggacc gccaatacca gctgttgaaa agctaattgg gacattgctg attacctctg
    13801 tacggtcaat caagccgatt ccaattccat ttttgccatt ttccaaggtg ataatctttc
    13861 cttctgcaga cttggtttgc ccatcctctt cataggtgac cttgacggaa tcccctaatt
    13921 tttgagaact gacgtaatca atcaagtctt tggaactatc aaaggtctga tcattgactg
    13981 ctgtgactgt atcagagata ttgagaatcc ctttaaaggt tgaattatcc gtcacattca
    14041 aaacataaac tccaaagtac ttgagttcga tatccttacc agctgttttt agtccttgat
    14101 acttggccat attttgcgat gtttgcatgt agaattgatt gattcgcata aattcaacat
    14161 cggaagaacc acctgtagtc tcctgagcac tacgaatatc tgtaaaaggt gtcaaccaag
    14221 cataaatcat atgagctaaa gtggcatgtt gaacaccaac cgtaacgaat tgataggcac
    14281 cagcttcctt atcttctgtg tcatttattt taaggacttg gcgaatatct tccgaaccac
    14341 ctggaacctc tatataataa ggcaagggca ctacaaatgc caagaaagtc acaatcaagg
    14401 ccgcaatgac gtataagggc catctaatct tttttttcat ttcttatttc ctccaaaata
    14461 ctctcgggaa catagcaggc aatatcctga ccaaacttca aaagctctct aacgcctgat
    14521 gaactgatat agagatgttc aggtcgacta tgtaaataaa tagtctctat atcagaagac
    14581 agctgatgat tgtagtaatc aaaactggct tcatattgca aatccgacgc attcctcaaa
    14641 ccacgcacta gacaagtagc acccaatctt tttgcaacat cgaccaccaa ttcatcatga
    14701 gaagccacga cttcaacatt ttccagatgt cccaaagcct tttctagccc ccgtttacga
    14761 ttttcgatag gaagaaatcc ttgtttgtgg ggattaaaaa aaataccgac atagagctta
    14821 tcaaagagtc tgctcgcccg ttcaatgata tccagatgcc catttgtcat cggatcaaat
    14881 gagcctgtga ataagccaat cttatctgac atagactgtc accttactaa ttccataaat
    14941 cttttccttc cagataccca gacaggcaat ttcttctgga agttcaacgg ccttatccgt
    15001 ctcgcacaca accataacat cttcagaaaa aagctctctc tcagccattt tttcaatatc
    15061 tgctacgatt tgttccttgg tatagggagg gtctaagaaa acgaggtcaa attccccaga
    15121 tacctgttcc aatgcccttt ctgcatccat cttgaggagt tgaaattttc caatttcctt
    15181 ggttatctgg atattttcag ccacgatggt ctgagcctta cggtctcgct ccaccaaaac
    15241 agcactggac atgccacgcg atactgcttc gatagataaa ccaccactac ctgcataaag
    15301 gtccaagact cgtccccctt caaagtaggg accaatcatg ttaaaaatgg ctcccctaac
    15361 cttatccgaa gtaggtcttg tcgtcttgcc ttctagtgtc ttgaggggac gtcccccata
    15421 gattcctgat acgattttca tactgtttat tataccaaat tatagacaaa aagagaaaga
    15481 aaaccgaacc ttgcggttcg attctctaca aaatattttc gtaagtatcg cggacttctt
    15541 gaggccaaac acttgtttgc acttctccga tgtgtctctt gcgaagtagg aacatggcca
    15601 tacgagattg tccaattcct ccaccgattg tcaatgggaa tagaccattc aacaaagact
    15661 tgtgccattc caattccaag cggtcttcat cacctgtaat ttccacctga cgtctaagag
    15721 tttcttcatc tacacgaatt cccatagaag acaactcaaa ggctccacct aaagactcat
    15781 tccagacaag aatatcacca tttagaccct tgtagccatt ctcagactcg cttgtccagt
    15841 catcatagtc tggtgcacgt ccatcgtgcg gtttaccatc tggcaactcg ccaccgatac
    15901 caatcaaaaa gacggctcca aattctttac aaatcgcatt ttcacgttct ttcggtgtca
    15961 agtctgggta gcgttctacc aattcttctg tatggataaa ggtgatttgt tttggcaaga
    16021 tagactcgat gtcatagcgg gcttcaacag ctagctcagt caggcgaata gccttataaa
    16081 tcttctcaac tgtttctttt agataaacga tgttacgctt accatttggg ataaccttct
    16141 cccagtccca ctggtcaaca taaacagagt gggttgcatc caaggaatcc tcatctggac
    16201 gaagggcttt catgtggaca aagagaccct ctccttcacc aaagccaaaa cgagccaagg
    16261 tatggcgttt ccatttagca agtgagtgca ccacttcata agtagcatca gggatttgga
    16321 gaaccttgac cgatactgga ttttccacac cagacaggtt gtcctgcatt ccgtcaccga
    16381 ccttactcaa gataggacct tgaacttcga caacttctag cttatctttc aaatactggg
    16441 taaaagtgtt tttgacaaag gaaatttctt cttgttgatg gataaaactt ttcttcatag
    16501 acaactcctc aaaaatttaa taaacgtatt atacacctat ttccaagaaa agaaaagaga
    16561 aaatttaaaa aaatcagtgt cactcaaaac actgatttca tagacctttt agtcattgcg
    16621 accaaagata cgtagaatac ttaggaaaag attgataaaa tcaagataga tgctgagagc
    16681 cattgacacc acccaacctg tcgctacacg accttgtgat tgttcatagg cgaggcgaat
    16741 cttttggtta tcccaagcaa tcaaccctga aaaaaccaaa accatggcta cgctaatcat
    16801 ataatcaaag aagccactag ccaagaaaat attgactacc atagcaatca gtaaaccaat
    16861 gagagctgcc atcattgctc gaccaatccc actcaagtct ttcttggtaa agataccaac
    16921 tgccgccatg acaaagaaga gaagggcgct cgatacaaag gcagataaaa ctgtacccgg
    16981 agtatagaaa gccaccacaa aactaagggt aaagccgttt aaaacggagt aaagtaaaaa
    17041 tactggaaga gcagctggac tatttcttga agccatgcta ctggcaacga aaaccaaagc
    17101 caactccgca aaagtagcaa tggtcaacca gagacgcccc tgcatcaaaa agtaaaccaa
    17161 ctgagactga aagaccgtca acataaggcc tgataccaaa gcagatagtc cgattcccag
    17221 accaacaaag gcataaacct tagcgtaaaa ttgattgaga cctgcacggt catgtataat
    17281 agtgtgattc atccatttct ccttttctgt gaacatcttt ccttgattat aacaaaaaaa
    17341 gttaaaatta gcttaaatgg aagattaaaa taccggctgc aaccgcaaca ttcaaactct
    17401 cggcctgccc cttcatgcta atatggacca actggtctgc actttcagcc ataaggggac
    17461 taattccttg accctcattt cccataacta gtacaaaatt ttctatagaa ggcagttctc
    17521 tgtaatcaac agaatcttta gatagggttg ttgctagcac tgggatagct acctttttag
    17581 tttcgtcaag aagcgcttga ctagtcatcc ggtaaatggg cagatggaaa tgactacctt
    17641 gcatggaacg tagggttttg agactgtaga tgtctgccga cttatctgaa acaatcactc
    17701 ctgtaaaacc tgctgcatcc gcagttcgaa tgatagttcc tacattacca ggatcttgca
    17761 catcttccaa aaacaagaac ttgccctgac tcaaatcagc ttgtcctact tcttctcttt
    17821 gaaccacggc aacaattccc tgtggagtct gagaatccgc caaatctagc aaaatatcct
    17881 ctgacaccca gacagtttgc gaaaaagtag ctaactgatc tcgataattt tctagggtaa
    17941 agattttctc aatcgtcact ccagcttgaa cagcttcttc aaacaagtgc cagccttcaa
    18001 tcaaataggc tgacttgcgg tatttttttt ggtgtaattt cttggtattt tttaccacag
    18061 aattggcttt tgaggttata atagtcatag aaatattata acacaatcaa aggggtttgg
    18121 tatgcaaaag gttagaatga ttgcccaagg tagggtacag ggagtcggct ttcgttgggg
    18181 tgtttacagc ttggcccttg aaatcggtgg catcacaggt cgggtatgga ataacgacga
    18241 tggcacagtg gaaatcttag cccaagcaga ctcatctgct atcatggcaa aatttatcca
    18301 agaaatccga aaaggaccga cacctttttc aaaagtaagc tacttagatg tcaaactaag
    18361 caactttcct ccctactctg actttaaaat cgcaaattag gtctccagaa ctattgtata
    18421 ttttgtaaaa aaacagtaga atagaaaggt ataattttta aagaaggaat aaaaacagtg
    18481 aaatctatta aacgttttgc attctcggct atgggagtgg ctatgttgct tgtcttgact
    18541 ggctgtgtca atgtcgataa aaccacaggt cagccaacag gatttatttg gaatacgatc
    18601 ggagcgccta tggctgaagc catcaagtac ttcgctactg ataaaggtct aggctttggt
    18661 gtcgctatca ttatcgtaac cattatcgta cgcttgatta tcttaccact tggtatctac
    18721 caatcatgga aggcaacgct tcactctgaa aagatgaacg ctctcaagca tgtccttgag
    18781 ccacaccaaa cacgtctcaa agaagcaact actcaagaag aaaaactcga agcccaacaa
    18841 gccctctttg ccgctcaaaa agagcacggc atcagcatgt ttggtggtgt aggatgtttc
    18901 cctatcctcc ttcaaatgcc tttcttctct gctatctact ttgctgccca acatactgaa
    18961 ggggttgctc aagcaagcta cctaggcatt cctctaggtt ctccaagtat gattttggtt
    19021 gcctgtgctg gtgtccttta ctatcttcaa tcgctccttt cacttcacgg ggtagaagat
    19081 gaaatgcaaa gagaacaaat caagaaaatg atttacatga gcccactcat gatcgtcgtc
    19141 ttctccctct tctcaccagc cagtgttaca ctttactggg ttgtcggtgg tttcatgatg
    19201 attctccaac agtttatcgt caactatatc gttcgtccaa aacttcgcaa aaaagtccgt
    19261 gaagaactag ccaagaaccc accaaaagca agtgctttct ctaaaccaag tggacgaaaa
    19321 gacgttaccc ctgaacaacc aactgctatc acaagcaaga aaaaacacaa aaatcgcaac
    19381 gctggaaaac aacgttcgag ataagaagaa aaaggcttag ctgatttgct aagtcttttt
    19441 gcaaatcaaa agcccgatgt ttccatcagg cttcttttct atccatgtac acgtttcata
    19501 taatcttgat aactttcggt atccatgagt tttttagcgt tcttgacgcg atctgctgtt
    19561 ggtggtttga ctccttcgag ggaatatgga attccaagtt cacgccactt gaactcaccc
    19621 atggtgtgat aaggtagaat ttcaaactta tcaacatttt tgagggtctt gacgaactta
    19681 ccaagttcaa tcaagtcatc atctctgtct gtcaatcctg gaactagcac gtggcgaatc
    19741 cagacaggtt ttccaatatc tgatagatac tgggcacaag ccaagatatt tttattagtt
    19801 tgtctagtga caatcttgtg ctgttcttcg ttgatttcct tgatatccaa aagaaccaag
    19861 tcagtgacag ccatgagttt gtcaaacttc tcaaggtaac gtggtttatt acggaaagga
    19921 agagcacagg tatccaaggt acagtggatt ccttgttcct tagccttggt gaagagagca
    19981 atcaggaaat caatctgcaa gagagcttct cctccactga ctgtaatccc acccttattt
    20041 ccccagaaac cacggtagcg caaggcctct gtcaagacat catctaccgt ccgttcacgt
    20101 gacttattgg actccatagc ccaagtgtct gggttgtggc aatactggca acgcatgtga
    20161 cagccctgca aaaagacaat aaagcgaata ccaggcccat ctactgaccc aaagctttct
    20221 gtcgaatgca ccattcctgt cacttgtcca taatcaattg tttcttcaga catatcgtta
    20281 ccttccttga aaacgtttta tagttttatt atatcatgac ttgaaggaaa aacaagattt
    20341 ttgcctattt tttagaaagc aatctgtttt tcaatttcat tttcaatcta ttatcagatt
    20401 attcttcaaa atcgaagcca tataaggttg caaaatagtc ctcagggcgc tctgcacggc
    20461 ggatttgacg ggctttacct tcttcggtat agaggatttc cgcagaacgt aatttggcat
    20521 tatactggta gcccattgaa aatccgtggg cacctgtatc atgaatgacc agcaaatcac
    20581 cgatttctgt atgaggcagt tcgcgattga ctgcaaattt atcattgttt tcacagagtg
    20641 aaccaaccac atctaccgct tcagctggtc catctggatg ggtcacgttg ctaatatgat
    20701 ggtaagctcc gtacatagct ggacgcatga ggttgactgc tgaggcatcc acacctagat
    20761 aggtacggta ggtttccttc ttatgagtca ctcttgtgac tagagcaccg tgaggtgcca
    20821 gcataaaacg acccaattcg gtgaaaatct tgacctgacc aagacctgct gacgtaagaa
    20881 cttcttcata caccttacga actccctcac caatcaaggc gatatcgttc ggctcctggt
    20941 ctggacgata attaacacca ataccgccag aaagattgat aaagtctagc gaaatgccca
    21001 acttttcctt gatttcaaca gccagttcaa agagctgacg agccaactct ggataataga
    21061 gatgggtcac ggtattggac gctaggaagg agtgaatccc aaaagttttg gctccttttt
    21121 ccttcaagat agcaaaggct tcaaagagct ggtccttggt catgccaaac ttagcctccc
    21181 caggattgtc cataatgtct gtccccagtt caaaaacgcc tccaggatta taacgacaag
    21241 agatgatttc tggaatgcct gctactctct ccagatgttc aatatcttca aaggcatcca
    21301 agttaatggt cgcacccaat tcacgtgcat aggcgtattc cttgtctggc gtgttgttgg
    21361 aagagaacat aatctcagaa cccagaaagt ccagtttatg gctcatcaaa agctctacat
    21421 aactagagca gtccacacca caaccttctt cttggagaat tttcaaaata gctggagttg
    21481 gagtagcctt aactgcaaaa tattccttaa agcccttgtt ccacgaaaaa gcttggttga
    21541 cggctcttgc cttctcacga atccccttct catcatacaa gtgaaaggga gtcgggaact
    21601 cggcaacaat cgcttctaac tcttctctat tgataaatgg tgttttcata agcttccttc
    21661 tattctgttt gcaaagaaag gctgggacaa tcgttccaac ctttagttct atctcttatt
    21721 tatcttcgtc aaagatattg ccaacctcaa catcgatggc ttggttcttg acatcaatat
    21781 tagttacctt caagagtgac ttgtagtcaa actgcttttc acgttcttct tgggcattgt
    21841 ccgcaaagac cgctacacct gccagttctg agtcgaactc gcgcaagaga ctaatcatac
    21901 cgttgaccgt tccgccacct ttcaagaagt catccacaat caagacacgg ctgcctgcct
    21961 taagactacg ttttgaaagg aacattttct cgatacggtc accacttgaa cctgaaacat
    22021 agttgacgct aacagttgaa ccttcggtaa ttttcaggtc acggcgcaca atgacaaaag
    22081 agacattgag gacattggca actgcatttg caagtggcac acccttagtt gctacggtca
    22141 taaccgcgtc aattttttgg tccataaagc ttttggcaat aatacgacca atatttttca
    22201 agatggctgg tgtgctaagc aaatcagaca gatagatata accacctggc aagatacggt
    22261 cactttctga caacttggta cgcaagtctt caaccatttc cttagcatcc tgactcgaaa
    22321 tagacggtgt gaagatgaca cctccgccag ccccagtcac tgtctggata tgaccgattt
    22381 caatttcctc aaaggcgcgt ttgataatga cgatatcttc tgagatggat gatttagcag
    22441 actcatactt ttcagcaaaa gtattgagac tagttagttt ataaggatta ttaatcaaat
    22501 agttggaaat gacaaccatc cgatcacttc ttcttaattt catttctatt tcctattcca
    22561 tttttattca aaaaatcaaa aagcaaacta gaaagctggt cgctggtggt tcaaaacact
    22621 gttttgagat tgtcgataga actgacaaac cctgtaatat acctgcatat atacatacga
    22681 caaagcgata ctaccctagt ttgaagatat tttcgaagag tattcatttt tgtcttttac
    22741 ttattatacc atattcacat aaaaaaacga acattcttat cctaaaaaat gctcattttt
    22801 cttaaattat caatctaaat ctggtttata gaaggaacga ttatccatag cgaagatttt
    22861 attggtcatc tctcctttat ccaccaaagc cagagctgtt gacatcatca tcatgcttgc
    22921 atccagattg tcaatcatat ggataatctc tgcctccata atacgtggac ggactggact
    22981 tccatattca agcaagccgt ggtgactgag gatgacatga cgaagcaaaa cgacttcttc
    23041 cttggtatca tcgatgccga gttccataac tgtcttggta atttcgctat caatgagagc
    23101 gatatgtcca agaagattac ctcgcactgt gtactctgtc tggtctggcc ccgtcaactc
    23161 gatgacctta gccaagtcgt gcaacataat ccccgcatag agcagactct tattgagctg
    23221 aggataaact tcgctaatag cgtctgccaa acgtaccatg gtcgccgtat gataagccaa
    23281 ccccgtttca aaggcatggt ggttggtctt ggcagctgga taggagtaga attccttatc
    23341 atacttggtg tagagatttc ggacaatccg ttgccagaca ggattttcaa ttttgaaaat
    23401 catttgcgac atgtagtcac gaatttcctt gacatcaact ggtgacttga ccttgaaatc
    23461 ggctgggtca ttgggttcac cagcttgagg caggcggaga gtaatttgat tgacttgagg
    23521 ggtattgtta taaacttctc ggcgtccttt catgtggaca accttacctg cggtaaaggc
    23581 ctcaatgtta tgaggttggg catcccagag cttcccatca atctcgccac tatcatcttg
    23641 gaaggtaaag gctaggtagt ttttcccagc tcgagtttgc ctcaggtcag ctgatttgat
    23701 taggtaaaag ccttcaaata gctcatcttt tttcatgtga ctaatcttca tattcttcct
    23761 cattttcttg aaaatggagt agatcaagcg caggctcgcc ttctgacaac tcaatgtgac
    23821 ggagcgtccg ctcgatagct atggtacgac ggtttaataa ttcatcaata ttgccagagg
    23881 catgttggag atgtttttgt gccttgacca gaataccacc aaacttgcca aactcggtct
    23941 tgacactggc aagagtcttg ctgatatggt cggcactctt ttggatatta agggtcttga
    24001 aaccaactga tagggagtta agaagggctg atagggtact tggtcctgca acaataatct
    24061 gttcttcccg tctcaaatca tcaaagaaga ccggattgcg gacgatttct gagtagagac
    24121 cttctgtcgg aacaaacaaa actccaaaat tggtcgtccg aggtggtgct atgtacttgt
    24181 tcctaatatc cctagcaaaa cgcttgacac ttgctaggag tgacttacga cagcgttcga
    24241 tttcatcctt gtcacctact tcataggctt cttctaagcg gtaataatct gccagtggaa
    24301 acttagagtc aattggtagg tagacgtatt cttggtcgcc ttgtccgggt aacttgatgg
    24361 catactccac tcgttcacta gagttttcaa ccgttgcgta ttctcgttcg tactgggcag
    24421 gtgtcatgat gtcttcaata atttgcccca gttgcaattc tcccagaatc cctcgcgtct
    24481 tggttccaga gagaaccttg ttaagagctc cgacatcacg ggcaactgtc tgcatttctc
    24541 caagaccacg attgacagac tccagttgtt tagaaactgt ctcaaaggaa gcctgtaagc
    24601 gtgtctgcaa ggtcttttct agtttttcct cgaccgtctg gcgcatttgt tccaaacgtt
    24661 gctcatttga ttcctgcaag gcttggagac gttggtctgt cttgtctcta gtttggagga
    24721 gattatctgt catttcttga cggacttggg tcaggccttg gtgcaattca atccgcactt
    24781 cttgcaaacg gtcgctgaca gccacttcca aatctttttg gtctaactgg ctggcttgtc
    24841 tggcttggtc aaagcggtaa tccaactggt ctgacaaatg atctgcctga tcctccaagc
    24901 tcttacttaa gtgtttctcc tgcctatcct gtctttgcca aatcagaaag agaccagcta
    24961 ggttggcaat taataataga ataagtaaac tttccatcct acctcctgtc cttgctatgc
    25021 agtacgacca cataaccatc tgggcaagtc accgacactt ccctatctat atattcgtta
    25081 gaagcgtaca cttttttaaa gaaaaaattc tcctctgtca actcatactt agctccaaga
    25141 atagtcagct ggctatcccg aactggcata aaggctagat agtcgtagtc tgaacgaggt
    25201 tctagctgac tggtcccttc tgaacaatag gcaatcaaat tttgcccatc ctcaatttct
    25261 atttgacgca tatagggtgc caacttagga ttgctaggta gaaagacatt ggccaacata
    25321 tggtcaatac ggccacccaa ggcaccgaaa atagtgacct cagcctgagg attttgttca
    25381 aagatggtta agagagccaa ttccagatct gtatcatcct tttctggtcg ggcttggaca
    25441 aaatgctggg cacgtttttg aatcacctgt cgctcttctt ccgtcacaga atcaaaatcc
    25501 ccaactgcta gagcaagaag caagtcttct tccaagaccc agagcgagcc tcgatccacc
    25561 ccaacaaaag catcaaaatc tgtccgataa tgaccgcggt ctccgcctgc aaaaacggca
    25621 accctagtcc agttgttttc tgagagtttg tactcgctca ttgacatctc ccttaaagac
    25681 ataggaacct gctacaaaaa cggtcgcacc agcttcttta gcttgggcaa tagtttggtc
    25741 atcaatccca ccatccactt cgatttcaaa gttcaaacct ttttcctcac gaagagcaac
    25801 caactcacga accttatcca tggtttctgg cagaaaggct tgcccaccaa aacctggatt
    25861 aaccgtcatg actaaaactt ggtcaactag atgaaggacg tgcttgatgg cttcaactga
    25921 tgtgccagga ttgataacga ctgaaggctt aactccgagt gaacgaattt tttggagggc
    25981 gccatgaata tgaggcgttg cttctacatg gatactgatg atgtctgcac ctgcacgcgc
    26041 aaaatcttcc agatgatgct cagggtttga caccatcaag tggcaatcga aaaccatctt
    26101 actatgagga cgaaggctct cgaccacacc tgcaccaaaa ctgatttgcg gtacaaaatg
    26161 actgtccatg atatcgatat gggcatattc tgccccagtt gcttctagac gtttgatttc
    26221 acgttcaaag ttggcataat ctgctgccag aattgacgga gcaatcttgt attgagacat
    26281 aggtttctcc ttattttgga atttttttgc tgactttttt ataggtttct ctacgatttt
    26341 caatttcact aaggaattgc aggtaattgt caaaacggaa ggttgcaata acaccctctt
    26401 caacagctgg tttgacggca caagacggct catgggtatg ggtacaagta cggaacttac
    26461 aatctcggct aacagtagca atctctggga aagcctgatt gaggtcttca gcccttgata
    26521 cttcatagtc caaggatgaa aatcctggtg tatctgcgat tttacccccg ttgagattgt
    26581 aaaaactaac agctcgagtg gtatggcgac cgcgacctag actgtctgaa atttctcccg
    26641 tttcaagatt gaggtctggt gcgattttat tgagaagagt tgacttccca acacctgtct
    26701 gccccataaa gaccgtaacc ttgcctgtta acaaagacag gagttcctct ttactggtca
    26761 caaagtcata gccgatgtca ccataggtct gctggtaaaa atccagttct cccctatctt
    26821 ccaacaaatc cattttggaa atatagacaa tgggatggat gcccttgtgc tccaaaagaa
    26881 ccaagaaacg atccagcaaa ttgctgttaa aatcaggttc cttgacggac atgattacta
    26941 cagcttgatc gatattgaca ataggcggac gaaccagact gtttttccgt tcgtgaattt
    27001 tgaggatata gccttctgaa ttttcctcgg cagagaaatc tacccagtcc ccaacataag
    27061 gggtatggcc ttttttacgg aaattcccac gcgcgcgtgt ttgataaacc tggccatcac
    27121 tctccacata gtagaaacct gccaaggctt taatgatttg tccctgcatc ttagagtcct
    27181 tgccctttaa gtgcgtctgc tagaccggca aattctgcca agctgagagc ttccccacgc
    27241 acacttggtg acaagcctgc ctggtccaaa gccttggtca gcttgtcctt gacctcttca
    27301 gtcttaccaa agtaacctgt caagttattc cacaaggtct tgcggcgatg ggtaaaacta
    27361 gccttggaaa ccttaaagaa aaagttctca tcttctactg ctacggctgg ctctggacga
    27421 cgcaccattt tcaagatggc tgaatccaca tttggcgctg gcacaaagac cgtacgaggc
    27481 acgataaagg caaccttggc tgtcatgtaa tactgcacgg cgatagacaa gctaccgtaa
    27541 gccttggtgt taggctgggc tgaaatgcgg tccgctactt ctttctgcat catgaccaca
    27601 aactcactaa aaggaatgcc actctcaatc aagtgcatga gaataggcgt cgtgatgtag
    27661 taaggcaaat tagccactac cttgattggc aggttaggat ttttaaaatt ttggatatgt
    27721 tgcgccaaat caaccttgag aatatcttcg ttaactacag tcacattatc aaaatcacgc
    27781 agggtatcta ccaaaattgg caccaaacgg tggtcaatct caaaagccat gacttgggct
    27841 gcacgctcag ccagaaactc cgtcaaggca ccaatacctg gcccgatttc gatgacattg
    27901 acctggtcat caatttcagc cgtatccaca attttttgaa ggatattggt atccgtcagg
    27961 aaattttgcc cgaaggactt tttaaaggta aaaccgtgac gctccagcac tgccttggtc
    28021 acgctataat ctgcaattct catctattct cttttctatt atctgttact actattgtaa
    28081 cacattcccc ttgtattttg ggaatgaaaa tcataccaaa aaactatctt tcctcatgaa
    28141 gaaagatagt cttggaaatc tttaaaagtg gccttaacag ttctctcgaa acatgcgttc
    28201 atactcttcg aaaatctctt caaaccacgt cagcttcacc ttgtcgtact caagtacagc
    28261 ctgcggctag cttcctagtt tgctctttga ttttcattga gtatcaaaaa aatcagcttc
    28321 ttgataactt ctattttcgg gtagggtcaa atccacatcc tcctccacat catccactgt
    28381 cagttcaacc gtttgaggta aatttttcaa acgataatgc ttttgccttt acccttcaat
    28441 actagcaaaa tcaagcaaat agccatgcac taggatttga accaaaagga gatgattggt
    28501 catattttta tcttctaaac cacctctggc actaggataa tcagataccc tgcgaatacg
    28561 gattttttca cacatgagca agtttcctca tatcaatgat ttcatcagcc ttattccaga
    28621 ctaggggatt atgggtcgca atgatgataa ttcgattttc atctttcaaa tccaccaaga
    28681 gattcataac ctcctctgaa ttttcaggat caagagctgc tgttggttca tctgccaaaa
    28741 tcaagggtgg atttttcaaa atagtttttg caagggcaac tcgttgggcc tctcccccag
    28801 ataaagtata gattttttgt tctaaatcca aataccctag attaactttt tctaaagccc
    28861 ccacttgcct ttccaaacgt tctacttttg agattttctg accaacaaaa cccaaatcca
    28921 aattttcttt gattgattgg ttttctaaga ggccgaaatt ttgaaagaga tagcccatct
    28981 ggtctcgaaa atactcacga gtaggaatgg tttttaaatc tttcccctga tagagaaccc
    29041 ttccaccatc tatcttttct agctttccca agatattcag cagcgtcgtc tttccgcttc
    29101 cactctttcc gattaaggca taaaccttgc ccttctccag cttgagattc aaaccagaga
    29161 aaatcgcgcg gtcattaaat ttcttttcca atccttgaat atcaatcatc ttattttcct
    29221 ttcaatactg ccatgctacc agcttcttct tttttgtcct gccttactaa aatcaagagg
    29281 gcgttaagcg taaacaaagc tacaactaga gcgctaatca aaccatctct tgtcaaaata
    29341 ctagatagga ctaggccaag caagagaact cctccttgcg ccagtaaata cttgccatga
    29401 agttcataga ttgtcatacc agcaagacgt ttaatcataa gctcccgtct gaactgctca
    29461 aaatagagaa gattcatgga atcaaataac aagatagccg tagacagggt caaaatcgtc
    29521 ccaataagga gagagtaaaa ctccacccgt ttgtcattta gtaccttggc aaaaaatagc
    29581 tggcttttta ccaagtaaga aaccttgtga tacagacctt gctcttttag agctgtgatg
    29641 ctgtctccat atcgatctag tttcaaggca ctattagccg tagttcccca caacatccct
    29701 gcaacaggtc ttgttccagt cgcttgcggc gttagaacta caataatcgg atcttttagg
    29761 tactggcgtg ttgtcttgta cccatcatta tagaggaaac gttcctgtcc tgtttctgta
    29821 aaagctagcc ttacctgtgg gaggtagtgt ttctgactcg tcacttctac actttcagat
    29881 gaaaagtttt gcaggtaatc tgtaaacaat ccttggtagt agacagactg ctctcgcaag
    29941 ctctcaggca agatcagccc aaactctccc tcagacaagt tttgcatctt gtccataaac
    30001 tctgaagaaa cggtaatctt ttcttctatc agatagcgtg gtgagacata gataacattc
    30061 cctgacggtg tgtagtcact gagacgattg ccatctaggt ccacttctgc tccatctgag
    30121 aaattgtcaa cattgctcat aatataaaaa gagtctgtat tggctaaccg ttcttcagta
    30181 aatgtctgcc actcacgatt atccttacgc gttccttctt catcggcaaa tgcactagac
    30241 caaccaaagg atagacggta acggtctgag gactggttcc atttattgct agctctctcc
    30301 atttcctgca tttcacggta gtggggtagg agagctgtcg cactcgatcc gaccaccaat
    30361 acagctaaga gttgccccac catcatcaat gtcatcatac gtttgagagg gagtttccct
    30421 ttcaatagat ccaccagact attttcctgt aaaccaagta gatagacgac acttagtaag
    30481 gtagaaatcc ctgccaaggt caatccataa agtagaagag caatgatgac cagttgcacc
    30541 gttgccataa acaaggcacc ttgataccag agaatcccca atcccaaaag actggatacc
    30601 agcactgagc agataagctg tctcacatct tctaacactg gtctgagagc aactccaaac
    30661 aagctctcac cagctattaa gcgaatccct gcctgacgaa gggatttgat ccgataaatc
    30721 agggtcagac tcataaaggt cagcagaaaa atagctaaac tcagtagcac cataggggtg
    30781 gccgtcaata gtaagactat cgaaaatgga tcttctccat tcgaaacaaa gccttgataa
    30841 ccaagctctt tcaaggtggt ctgtaagctc actccatcca aacttcctga tacaatcaag
    30901 taagacccta ataaatcact cgtttctgca ctctccttgg aggaaatggt cagaccttct
    30961 ggaagctttc cctcaccata agtggcatag gtaaagcgtg tttctccagc ttcatttggc
    31021 tcaacgattc gacgagcaat gagactattg tgctctaccg ccaagtgact caaagcctct
    31081 gtcacctctt cgcgactagc tactggttga tttatcatca ttcctgtcac atcaacagaa
    31141 ggatagttac caaataaaat ctcttctcga tggtcaacgg aagttatcac catataaagc
    31201 gatactaata ccattgaaat caaaataaat aaacgtttca tcatttcctc caaaaagtta
    31261 cttggtcact tagtgagaag gtgaccaagt aaagctaaac aatagggcta taccattcta
    31321 aacattatag tagaatgcaa ctttctctcc ccaagaagta cgaatccatg cacgagaagt
    31381 ccctccagca ccagcatatc ccttgtcaga tgaaccagtt tttctgctaa caactgttga
    31441 actatggtat tttgaagggt gatagtagtt tgagtaagca ccaccgttac ctgaaccata
    31501 agtccaaaca ccaccttctg ggtattgaac ggctgcagag gcaactgata cacctccacc
    31561 taatgttgct gcaatgctcg ctacaagagc taatttttta actgtttgtt tcatattgat
    31621 actcctctat ttttgattac aagattgttg agttacttgc tcctctttcc attatcaacg
    31681 ttgatactat tatattacca aatgatattc taaaattatt tgtttttctc taaattgtca
    31741 aaatagcacc ctgaaatgaa cttttatagt cattatactc catctttatc acactatcaa
    31801 ggacatcaat gtccttaaaa tctaactatt tcatattatt tttagcttat atctaaaaaa
    31861 acagttctaa agacgaactt tagaactgtt ttgatacgag gacaaggaca tttttgtcac
    31921 tgattcacta tgtttataaa tattttttga catcctcttg ccattcctct gtcaatttct
    31981 ttactagaaa tccatctcca attagcttcg caaatgtttt agaagactga taaaatgatt
    32041 cagctaaact aaaatctctt tctactctta atgcttgctt ccacaaaaac ataaatacaa
    32101 gaggtttctt ttgataatct tgagttttat ccataatttc ttgtaaacac gatagataat
    32161 cattaaaaca atcagttact cctacctttt caagacaaca cattcctgaa ataagagtat
    32221 ctctaattat aaagtaattc tctatttgag ttaccttctt ctgttccaaa agaaagagca
    32281 ttagcttttc gatttgattt ataaatttgg caagatcttt tctaatgagc atttgataaa
    32341 aaaacaaacg aatcaacaac atatcattta ttgaataagc ttctttttct ataatctgag
    32401 gcatatgttc ctcaagtatt aactctgcat attcaggcct tctagttcta gaagtatcca
    32461 aacacgcctg caatacttca catgctaatt gttcttcttt aggaagatta tcaaagtatg
    32521 ttttataaat ctcttccaaa cactcttcct tcttatcgta ctcctcttct ttaccataga
    32581 ttggttcacg taaaatctgg tatttcaatt ctaaataagc agaaggaaga gctgaaaaat
    32641 ccggcataag gctataaact ggaactccta gccggcgagc aatatagtct aacttgggca
    32701 aactcggttg cgaagctcca ctttcaattc gcgacagttg acgaactgtc agttcttgct
    32761 catctccaca aaaatctgga cgactaattc ctttttctat ccgaatctct ttaatttttt
    32821 gaccaattaa catcagacgc ctcctcttct acctgcttcg aaaaatactg tgaaacttaa
    32881 aaaattgtaa cggctacatc tttcctactc ataagatttc atagcttctt ccacttctgc
    32941 caaagtaacc ccaaacaact ctaggcgttt gaggagttgc ttgccgttgg aatagccgat
    33001 tcggagagtc tctccgaggt attctctacg cttacggctg tctgcccctg ctagaaaacc
    33061 aaggcgaatc aaatcgctac gactaatgtc aaactgactc tcatgttcaa attgttctgt
    33121 cacctgagct agagccgttt tcaggtcttc atagctggca tgctcaattc ccagagaacg
    33181 ccccttggtc ttggacttgg gaacagcttc atctcgcttg agaaaggcat gctgaactgt
    33241 tggaatgacc gtcatgatca tgcgccgaat ccgttcccca ttaaaatctg ggtctgtaaa
    33301 gacaatgact ccatgacgtt ggtgcaggcg ctgaatccgc tctatatcct gatcattgat
    33361 ggcagaacct cgagtctcat aggtctccac atcgaaataa cgcttgagat tgaccgtatc
    33421 atcacgccct tcaaccacaa ttacttggga aattttctct ttcattactt gctgtccaat
    33481 ccaaaaatgc gttctgcatt tgcagtcgtt gccgccgcta gctcttctgt cgtcatacca
    33541 cgcaagtcag cgataaagtc gaccacatag cgagtatagg ctgttttatt ttcacgacca
    33601 cgcttgggta caggtgctaa gtaaggcgca tctgtttcca ccaacatctt gtccaaaggt
    33661 aactctttag ctgcttcttg gaggtcagtt gccttcttga aagtcaccac tcctgagaag
    33721 gaaatggtca taccaagatc cacaaacttc tctgcccact caagcgtccc tgaaaatgaa
    33781 tgcatgatac caccacgagg accaacgccc tcactcttga taatctcata ggtatcttcc
    33841 agcgcatcac gggtatggac aacaaaaggc aaatccaagt ccttagatag ctgaatctga
    33901 cggcgaaaaa cctgcttctg cacctctttg ggcgctgtca tccaatggta gtctaagcca
    33961 atttcaccta aagccacaac cttggaatgt tttaacttat ccaacaagta agcctcaact
    34021 tcctctgtat aagtaccagc ttctgtagga tgccaaccaa tagtcgcata gagctgctca
    34081 tactcatcta ccaactccaa ggcatgctca atcgtcggtt tatcaaaacc aacaatattc
    34141 atctgtgtca cacccatctc atcagccaag gcaatttctt ctgcctcacg acctgcaaat
    34201 tcttctacat tcaagtgtgt atgtgtatca aaaatcatct cttctaacct cgttttctat
    34261 cttctattat accaaaaaag aggaggggca cctaattttt cggtttcccc tcctctcttc
    34321 aatagagagc tattctgcta tcttttctat ccgatattgc ccatctcctg tcccacagtt
    34381 agagacaaaa gagattggct acattgatag aatattccta ttcagctaaa gtatgtgatt
    34441 atcttgggtt ctcacttttc tcacccatca ctaggattga taacttctca aaatccccct
    34501 tataatctaa ctttaagtag agaatagaat tttctagctg ttctattttc tacgtctagt
    34561 ctttcttcct agcaaagcaa aaccacttgt tgccaccact agaccaagac tagcaagtgc
    34621 tgcacttgat gctgaacctg tgttaggtaa ttgtttgcca gcaggtgact ggtaggtaac
    34681 atcttgcatt gttcctgcag ttgcttgcgg tgtacttgtt gtcttaggag cagcattagg
    34741 agccactggt ctcttaggaa ctgtaaccat gacaatggct tgcactccaa tcgtttctgc
    34801 ttccttcaca tcttccatag ttttcgcatt ttcaatctct ttgagagctg cttgtttttc
    34861 tgcttccact cttgctaaaa gttctgcttt ttctttatca gaaagcggtg cgcctttgat
    34921 ttcatcctgc ttgtccttgg ctgctttttc aatggcattt ttagctgatt ctttttcagt
    34981 agctaattgc ttagaagctt gtgattgagc tgagttttgt gaacctgtgt gagctgatcc
    35041 acccgcctgg ctaccaccgc ctgcattcgc attagcatct gagcttccag tattgcctcc
    35101 cttatctcca cctggtgtat tttctagctt atactctggt gtagcttctt gagttacaga
    35161 ttcacctttc gcagattgtt gaactgtatc ttttcctaga atcgtcacta ctgaaccatc
    35221 tgggaatgtg attgttgctg taccatctcc agctacattg attgtcgctc cgtctaatgc
    35281 tgaaccattt gcttgtaaaa tagcaacctt aactttttct tcatctgtta aatgagttat
    35341 atcttttaca tctactcttt gagcaggtgc tttaacagtt gatttttcag cagtcggttt
    35401 agtgctatct tgtgcttttg gatcttgtgc taccaattga tttcctggaa ttgtaatagc
    35461 tgaaccatct ttaaagacga ttgtcgctgt gccatctcca gctacttcaa ctttatagta
    35521 aaatgaaata ataacaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt
    35581 tagaaataga ggtgtactat tctagtttca atctactata acatcgcttc caaatccatt
    35641 tgcatctact gaactttttt acaaaaataa aaatcagtag actcgagttc ctactgattt
    35701 ttgatagaat tcgtttggat tatgcagcca aaactttgcg tcctttacga cgacgagctg
    35761 ccaatacgcg acgaccattt ttagttgaca tacggttacg gaatccgtgt ttgcgcgcac
    35821 gacgaagttt acttggttga taagtacgtt tcacgatgaa tacctcctca tagattttgt
    35881 attcgtttag ccggctataa agtgatcagt tactaaacat actttactat tctatctgat
    35941 tctttcactt ttgtcaatag ctctaggcaa atgtttccag ctttttcgaa tgcaagcc
//
LOCUS       DAVDWM010000016        35373 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVDWM010000016 DAVDWM010000000
VERSION     DAVDWM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35373
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.016"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..106)
                     /locus_tag="VUW49_000985"
     CDS             complement(<1..106)
                     /locus_tag="VUW49_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET7980918.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTA"
     gene            101..355
                     /locus_tag="VUW49_000986"
     CDS             101..355
                     /locus_tag="VUW49_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7980919.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            650..1984
                     /gene="trmFO"
                     /locus_tag="VUW49_000987"
     CDS             650..1984
                     /gene="trmFO"
                     /locus_tag="VUW49_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET7980920.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2068..2805
                     /locus_tag="VUW49_000988"
     CDS             2068..2805
                     /locus_tag="VUW49_000988"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7980921.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2814..3371
                     /gene="frr"
                     /locus_tag="VUW49_000989"
     CDS             2814..3371
                     /gene="frr"
                     /locus_tag="VUW49_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7980922.1"
                     /translation="MANTIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3431..4285
                     /gene="cvfB"
                     /locus_tag="VUW49_000990"
     CDS             3431..4285
                     /gene="cvfB"
                     /locus_tag="VUW49_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET7980923.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGCVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4294..4509
                     /locus_tag="VUW49_000991"
     CDS             4294..4509
                     /locus_tag="VUW49_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET7980924.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4595..5563
                     /locus_tag="VUW49_000992"
     CDS             4595..5563
                     /locus_tag="VUW49_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7980925.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5541..5720)
                     /locus_tag="VUW49_000993"
     CDS             complement(5541..5720)
                     /locus_tag="VUW49_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980926.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            5756..6256
                     /locus_tag="VUW49_000994"
     CDS             5756..6256
                     /locus_tag="VUW49_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980927.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6259..6519
                     /locus_tag="VUW49_000995"
                     /pseudo
     CDS             6259..6519
                     /locus_tag="VUW49_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6886..7212)
                     /locus_tag="VUW49_000996"
     CDS             complement(6886..7212)
                     /locus_tag="VUW49_000996"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980928.1"
                     /translation="MVDVAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTNLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7158..7571)
                     /locus_tag="VUW49_000997"
     CDS             complement(7158..7571)
                     /locus_tag="VUW49_000997"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET7980929.1"
                     /translation="MGAHFLTKQAGGSGVLLGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLMSNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(7572..7997)
                     /locus_tag="VUW49_000998"
     CDS             complement(7572..7997)
                     /locus_tag="VUW49_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7980930.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8174..8743
                     /locus_tag="VUW49_000999"
     CDS             8174..8743
                     /locus_tag="VUW49_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7980931.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRC
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8810..9460
                     /locus_tag="VUW49_001000"
     CDS             8810..9460
                     /locus_tag="VUW49_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET7980932.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9444..11684
                     /locus_tag="VUW49_001001"
     CDS             9444..11684
                     /locus_tag="VUW49_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET7980933.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFFTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFIFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNTWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKNSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPYQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11863..12051
                     /locus_tag="VUW49_001002"
     CDS             11863..12051
                     /locus_tag="VUW49_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980934.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12083..12670
                     /locus_tag="VUW49_001003"
     CDS             12083..12670
                     /locus_tag="VUW49_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980935.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12674..13855
                     /locus_tag="VUW49_001004"
     CDS             12674..13855
                     /locus_tag="VUW49_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980936.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    14015..14146
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14162..14692
                     /gene="infC"
                     /locus_tag="VUW49_001005"
     CDS             14162..14692
                     /gene="infC"
                     /locus_tag="VUW49_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7980937.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14725..14925
                     /gene="rpmI"
                     /locus_tag="VUW49_001006"
     CDS             14725..14925
                     /gene="rpmI"
                     /locus_tag="VUW49_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7980938.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14977..15336
                     /gene="rplT"
                     /locus_tag="VUW49_001007"
     CDS             14977..15336
                     /gene="rplT"
                     /locus_tag="VUW49_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7980939.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15394..15774
                     /locus_tag="VUW49_001008"
     CDS             15394..15774
                     /locus_tag="VUW49_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7980940.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYKDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16024..16824
                     /locus_tag="VUW49_001009"
     CDS             16024..16824
                     /locus_tag="VUW49_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET7980941.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16787..17773
                     /locus_tag="VUW49_001010"
     CDS             16787..17773
                     /locus_tag="VUW49_001010"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET7980942.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKEFLR"
     gene            17815..19848
                     /locus_tag="VUW49_001011"
     CDS             17815..19848
                     /locus_tag="VUW49_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET7980943.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGAIGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19909..21564)
                     /gene="pavA"
                     /locus_tag="VUW49_001012"
     CDS             complement(19909..21564)
                     /gene="pavA"
                     /locus_tag="VUW49_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET7980944.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21669..22166
                     /gene="ybeY"
                     /locus_tag="VUW49_001013"
     CDS             21669..22166
                     /gene="ybeY"
                     /locus_tag="VUW49_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7980945.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22147..22542
                     /locus_tag="VUW49_001014"
     CDS             22147..22542
                     /locus_tag="VUW49_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET7980946.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22559..23458
                     /gene="era"
                     /locus_tag="VUW49_001015"
     CDS             22559..23458
                     /gene="era"
                     /locus_tag="VUW49_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7980947.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23507..24331
                     /gene="mutM"
                     /locus_tag="VUW49_001016"
     CDS             23507..24331
                     /gene="mutM"
                     /locus_tag="VUW49_001016"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET7980948.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24331..24936
                     /locus_tag="VUW49_001017"
     CDS             24331..24936
                     /locus_tag="VUW49_001017"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7980949.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRKQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24923..26122
                     /locus_tag="VUW49_001018"
     CDS             24923..26122
                     /locus_tag="VUW49_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HET7980950.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26122..26271
                     /gene="rpmG"
                     /locus_tag="VUW49_001019"
     CDS             26122..26271
                     /gene="rpmG"
                     /locus_tag="VUW49_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7980951.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26311..26544
                     /gene="secG"
                     /locus_tag="VUW49_001020"
     CDS             26311..26544
                     /gene="secG"
                     /locus_tag="VUW49_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7980952.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26646..29000
                     /gene="rnr"
                     /locus_tag="VUW49_001021"
     CDS             26646..29000
                     /gene="rnr"
                     /locus_tag="VUW49_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET7980953.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTIIQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRISVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKSFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28963..29430
                     /gene="smpB"
                     /locus_tag="VUW49_001022"
     CDS             28963..29430
                     /gene="smpB"
                     /locus_tag="VUW49_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7980954.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29446..30306
                     /gene="tehB"
                     /locus_tag="VUW49_001023"
     CDS             29446..30306
                     /gene="tehB"
                     /locus_tag="VUW49_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET7980955.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIGQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30379..31332
                     /gene="coiA"
                     /locus_tag="VUW49_001024"
     CDS             30379..31332
                     /gene="coiA"
                     /locus_tag="VUW49_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET7980956.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31351..33153
                     /gene="pepF"
                     /locus_tag="VUW49_001025"
     CDS             31351..33153
                     /gene="pepF"
                     /locus_tag="VUW49_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET7980957.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33155..33868
                     /locus_tag="VUW49_001026"
     CDS             33155..33868
                     /locus_tag="VUW49_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET7980958.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33935..34876
                     /gene="prsA"
                     /locus_tag="VUW49_001027"
     CDS             33935..34876
                     /gene="prsA"
                     /locus_tag="VUW49_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET7980959.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKSNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34995..35336)
                     /locus_tag="VUW49_001028"
                     /pseudo
     CDS             complement(34995..35336)
                     /locus_tag="VUW49_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10643 a         6573 c         7983 g        10174 t
ORIGIN      
        1 aagctgtttt taatacagct aatatctctt caaaagtcgt gcgctgaaca ccaacaagac
       61 gcttaaatcg tgcatcagtt agttgtttac ttgcttcatc attcatagaa ctactatacc
      121 atattttgtt tcgcaggaag tctattggaa agtaagaaat attgaagctg aggctattag
      181 aagaaattgt gagcgtggtg ctattttttc aggtaaaata aaatatcacg aagattcaca
      241 gtttaaagga gatcactatg ttgaatgtta tgctgtttta gataatacgg ttatagcaag
      301 agatagaata acagtcccta tcgatccgtt atgtggaaaa gattttatag agtagcatat
      361 aattgattct taactggaat actcactatc tctttacatc aagaaaatga ctaaacaggg
      421 aagtttgcct tcttcccttt ttttgttata ctagtagaag aaaaaaatag aaagatttgt
      481 gggagtgaaa aagtccgggg gactttttca gcttgagcca agaaattcga gagcgaggaa
      541 agtctgggga actttttcag cctgagccaa gaaactcgaa agctcgtaag tctgattggc
      601 tttttcaatg tgaatcttaa cttcacactc ccaaagaggt attagtgtcg tgtctcaatc
      661 ttatatcaat gttatcggtg ctggtttggc aggttctgaa gcagcttacc aaatcgcaga
      721 gcgtggtatt ccagttaaac tatatgaaat gcgtggtgtc aagtctacac cccagcataa
      781 aacagacaat tttgctgagt tggtttgttc caattctttg cgtggggatg ctttgacaaa
      841 tgcagttggt cttctcaagg aagaaatgcg tcgcttgggt tctgttattt tggaatctgc
      901 tgaggctaca cgtgttcctg ccggtggagc ccttgcggtg gatcgtgatg gtttctctca
      961 aatggtgacc gaaaaagtgg tcaaccaccc cttgattgaa gtggtgcgtg atgaaattac
     1021 agaattgcca acagatgtta ttacagttgt ggcgactggt cccttgacta gcgatgccct
     1081 ggctgaaaag attcatgccc ttaataatgg cgatggcttc tatttctacg atgcagcagc
     1141 gcctattatc gatgtcaaca ctatcgatat gagtaaggtc tatctcaaat ctcgttatga
     1201 taagggagaa gcagcctatc tcaatgctcc aatgaccaag caagagttta tggatttcca
     1261 tgaagccttg gtcaatgcgg aagaagcacc gctcaattct tttgaaaaag aaaagtactt
     1321 tgaaggatgt atgcctatcg aagtcatggc caaacgtggc attaaaacca tgctttatgg
     1381 ccctatgaag ccagtcggtc ttgagtatcc ggacgactac acaggacctc gtgatggaga
     1441 atttaaaaca ccttatgcgg ttgtgcaact tcgtcaggat aatgcagctg gtagcctata
     1501 caatattgtt ggtttccaga cccacctcaa atggggagaa caaaagcgtg tcttccaaat
     1561 gattccgggt cttgaaaatg cggagtttgt ccgttatggt gtcatgcatc gcaattctta
     1621 catggattca ccaaatcttc ttgagcagac ttaccgttcc aagaaacaac caaatctctt
     1681 ctttgctggt cagatgacgg gggtggaagg ctatgttgag tcggcggctt cgggcttagt
     1741 tgcgggaatt aacgcagctc gtctcttcaa ggaagaaagc gaggttattt tccccgagac
     1801 gacagcgatt ggaagcttag ctcattacat tacccatgcc gacagcaaac atttccaacc
     1861 aatgaatgtc aattttggga tcatcaagga gttggaaggc gagcgtatcc gtgataagaa
     1921 ggctcgttat gaaaaaattg cagagcgtgc ccttgccgac ttagaggaat ttttgactgt
     1981 ctaatttttt tgaaagaatt gttcatgata ctataaaaat cttagaaatt gtgataaaat
     2041 aggtaggatg aaagaaggag agtgaaaatg gcgaatccca agtataaacg tattttaatc
     2101 aagttatcag gtgaagccct tgccggtgaa cgtggcgtag ggattgatat ccaaacagtt
     2161 caaacaatcg caaaagagat tcaagaagtt catagcttag gtatcgaaat tgcccttgtt
     2221 attggtggag gaaatctctg gcgtggagaa cctgcagcag aagcaggtat ggaccgtgtt
     2281 caggcagatt acactggaat gcttgggact gttatgaatg ctcttgtgat ggcagattca
     2341 ttgcaacaag ttggggttga tacgcgtgta caaacagcta ttgctatgca acaagtggca
     2401 gagccttatg tccgtggacg tgcccttcgt caccttgaaa aaggccgtat cgttatcttt
     2461 ggtgctggaa ttggttcacc atacttctcg acagatacaa cagcggccct tcgtgcagct
     2521 gaaatcgaag cagatgccat cctcatggct aaaaatggcg tcgatggtgt gtacaatgcc
     2581 gatcctaaga aggacaagac agccgttaag tttgaagaat tgacccaccg tgatgttatc
     2641 aacaaaggtc ttcgtatcat ggactcaaca gcctcaaccc tctcaatgga caacgacatt
     2701 gacttggttg tcttcaacat gaaccaacca ggcaacatca aacgtgtcgt atttggtgaa
     2761 aatatcggaa caacagtttc aaataatatc gaagaaaagg aataagaaag aatatggcta
     2821 acacaattat tgaaaaagct aaagagagaa tgacccagtc tcaccaatca cttgctcgtg
     2881 aatttggtgg tatccgtgct ggtcgtgcca atgcaagctt gcttgaccgt gtacatgtag
     2941 aatactatgg agtcgaaact cctcttaacc aaatcgcttc aattacgatt ccagaagcgc
     3001 gtgttttgtt ggtaacacca tttgacaagt cttcattgaa agacatcgaa cgtgccttga
     3061 acgcttctga tcttggtatc acaccggcta atgacggttc tgtgattcgc ttggttatcc
     3121 cagctcttac agaagaaact cgtcgtgacc ttgctaaaga agtgaagaag gtcggcgaaa
     3181 atgctaaagt ggctgtccgc aatatccgtc gcgatgctat ggacgaagct aagaaacaag
     3241 aaaaagcaaa agaaatcact gaagacgaat tgaagactct tgaaaaagat attcaaaaag
     3301 taacagacga tgctgttaaa cacatcgacg acatgactgc taacaaagag aaagaacttt
     3361 tggaagtcta aaaataaaca gaaaaactca gttggcattg ctggctgagt tttattcgaa
     3421 agaaggaaat atgaatacaa atcttgcaag ttttatcgtt ggactgatca tcgatgaaaa
     3481 tgaccgtttt tactttgtgc aaaaggatgg tcaaacctat gctcttgcta aggaagaggg
     3541 ccaacataca gtaggggata cggtcaaagg ttttgcatac acggatatga agcaaaaact
     3601 ccgcctgaca accttagaag tgactgccac tcaggaccaa tttggttggg gatgtgtcac
     3661 agaggttcgt aaggacttgg gtgtctttgt agatacaggc cttcctgaca aggaaatcgt
     3721 tgtgtcactc gatattctcc ctgagctcaa ggaactctgg cctaagaagg gcgaccaact
     3781 ctacatccgt cttgaagtgg ataagaaaga ccgtatctgg ggcctcttgg cttatcaaga
     3841 agacttccaa cgtcttgctc gtcctgctta caacaacatg cagaaccaaa actggccagc
     3901 cattgtttac cgtctcaagc tgtcaggaac ttttgtttac ctaccagaaa ataatatgct
     3961 tggttttatt catcctagcg agcgttacgc agagccacgt ttggggcaag tattagatgc
     4021 gcgcgttatt ggtttccgtg aagtggaccg cactctgaac ctctccctca aaccacgctc
     4081 ctttgaaatg ttggaaaacg atgctcagat gattttgact tatttggaaa gcaatggcgg
     4141 tttcatgacc ttaaatgaca agtcatctcc agacgacatc aaggcaacct ttggcatttc
     4201 taaaggtcag ttcaagaaag ctttaggtgg tcttatgaag gctggtaaaa tcaagcagga
     4261 ccagtttggg acagagttga tttagggagg cttatgagaa aatcatttta cacttggctc
     4321 atgaccgagc gcaatcctaa aagtaacagt cccaaagcaa ttttggcaga cctcgctttt
     4381 gaagaggcag cttttccaaa acatacagat gattttgatg aggtcagtcg ctttttggag
     4441 gagcatgcca gtttctcttt taacctagga gattttgaca gcatttggca ggaatatcta
     4501 gaacactagc atttattcat tgggtttggg ctagtaattt ctccatccct ctgctataat
     4561 aaaaagaaat aaaaggatta gagaggttct ttatttgaag gaacattcaa tagacattca
     4621 actgagtcat ccagatgacc tgtttcatct ttttggttcc aatgagcgcc atcttcgttt
     4681 gatggaagaa gagctggatg tggtgattca tgctcgtacg gagattgtcc aggttttggg
     4741 agaagagtct gcttgtgagg aagcccgtca ggttattcaa gctctcatgg tcttggtgaa
     4801 ccgtgggatg accgttggta cgccagatgt ggtgactgca attagcatgg tcaaaaacga
     4861 tgaaattgat aagtttgtcg ccctttacga agaagaaatt atcaaggata atactgggaa
     4921 accgatccgc gttaaaacgt taggtcaaaa actttatgtg gacagtgtca aacagcatga
     4981 tgtgaccttt ggaattgggc ctgcagggac ggggaagacc tttcttgcag ttactttggc
     5041 agtgactgcc cttaaacgtg ggcaagtcaa gcggattatc ttgacacgtc cagcggtgga
     5101 agcaggagag agtctaggtt ttcttccggg tgatcttaag gagaaggtag atccttacct
     5161 tagacctgtt tatgatgcct tgtatcagat tctggggaaa gaccaaacaa ctcgactcat
     5221 ggagcgtgaa attatcgaga ttgcgcccct tgcctatatg cgtggacgga ccttggatga
     5281 tgcctttgtc attctcgatg aggcgcaaaa tacgaccatc atgcagatga agatgttctt
     5341 gactcgttta ggttttcatt ctaagatgat tgtcaatgga gatattagtc agattgacct
     5401 gccacgtaat gtcaagtccg gtttgattga tgctcaagag aaactcaaga acatccatca
     5461 gattgacttt gttcattttt cagccaagga tgtggttcgc catcctgttg tcgctcagat
     5521 tatccgagcc tatgaatatt ctactgaagt tgcacacgac tgattttgag gaagttcgcc
     5581 tgcaaaagaa tatacttttg agagaggaaa atccagttgt ataggctaaa ggttttatcc
     5641 aaaggtttga gacaacgatt aggcacgatg gaaagaactt ttatgtggct gatgacgatc
     5701 agtgcatctt cctgtgtcat aatcacaggg cacaagaaag taggaatttg aaaagatgat
     5761 tgaccaacta tctaagtatt acagttgtag gatactaact gaaaaggata ttccaagtat
     5821 tttatcttta tatgaaagta atcctctgta ttttcagcat tgtccaccag agccaaattt
     5881 tgcaactgta aaagaggaca tgctttgtct acctgaaggt aaagctaagg ctgataagtt
     5941 ttttgttgga ttttggaatg gatctgacct tgtggctgtt atggattttg tctatgcata
     6001 tcctgatgag gagactgttt ttattggttt gtttatggtt gatcaagcct atcagaggaa
     6061 agggattggt agtcatattg tgacagaagc actagcttat tttgctaaga actttcgaaa
     6121 ggcacgtttg gcttatgtta agggaaatcc gcaatctcag catttttggg aaaagcaggg
     6181 ctttaaatca attggatgcg aggttaagca agaactctat acggttgtta tcgctgaaca
     6241 gagcctagaa gattagaaat ggcatcaagt aagaactatt tggaatttgt tttggaacaa
     6301 ttatcaggat tagatgatgt gacttaccgt tccatgatgg gggagtatat tctttacttc
     6361 cgcggcaaga ttattggcgg catttatgac gatcgctttt tagttaaacc cgtgcaagca
     6421 gtcttagata agattgacca atcttctttt gagtttccat acaaaggtgc caaagaaatg
     6481 atttgagtgg aagaacttga taataagatg tttctataag acctgatttt agctatgtat
     6541 aaccaactgc caacgcccaa acctaaaaag aaaaagcaag ggtgaacgaa gtaaaaaaga
     6601 agtctgctaa ggccctgtct ttgcacgggt aaaattttat atataaaaag aagctgggac
     6661 taaagagctc agcttccttt ggtttatata attgtcatta caagacgaag tggttgggcg
     6721 aaactctgtt gactttattc aatttagagt ttcttatgca caattgagtc tggaacgaaa
     6781 gtctccagtt gcaaagtata cagtacaata aaccaacgat gtaatagctg atgacataaa
     6841 gcacagtggg taggacttgc gaagtcaccc ttttcttttc aaaatttata ctaaatcatt
     6901 gatatcagtg tagtcacgat taagtccttg agcaactggt aggttagtca agtaaccttg
     6961 ataagtagtc acaccttgac gcaagccttc atcttcagag attgcttgtg cgaatccttt
     7021 gccagccaaa gcttcgatat aaggaagagt gacattggtt agggcgatgg ttgaagtgcg
     7081 ggcaaccgca ccagggatat tggcaacggc atagtggaga acaccgtgtt tttcatagac
     7141 gggttcatcg tgcgttgtca cacggtcagc tgtttcgata aggccacctt ggtcaacagc
     7201 aacgtcaacg atacagagcc tggacgcatt tgtttgacca tctcatctgt caccaattcc
     7261 ggtgcttttg caccagggat gagaatggct ccaatcacca catcagcatc tctcacactt
     7321 gcttcaatgt tgaatgaatt agacataaga gtttgaattt gacttccaaa gacttcttct
     7381 agaactgaga gacgcttggc actaatatct aaaatagtca cttgagcacc aagaccaagg
     7441 gcgatgcggg cagcatgtgt accgacgaca ccatcaccga tgatagttac ttttcctttt
     7501 ggaacacctg gtacaccacc aagtagaaca ccagagccac cagcttgctt agtaaggaag
     7561 tgagctccga tttaaacagc catacgacct gcaacctcac tcataggaac gaggagcggt
     7621 agttgtcctt gattgtcacg aacagtttca taggcaattc ctgttgtttt tgctgctaac
     7681 atagcatctg ctaattctgg agcagcggcc atgtgcaagt aggtgaagag aagaagatcg
     7741 tcgcgcaagt aaccgtattc agaacttaaa ggttctttta ctttcacaac caactctgct
     7801 gcccaagctt caccagcagt agcgacaatc tcagctcctt gcttttgata gtcagcatca
     7861 gtaaagccag aaccgagacc agcatttgtt tcgataagga cacgatgacc acgactaact
     7921 aagctatgaa cacctgcagg tgtgagggcg acacggtttt cgttattttt aatttctttt
     7981 gggattccga ttaacattga gataacctac ctttcaattg acggtcttgt tttggttgtc
     8041 acattccagt tcataaatca aaaatgtgac ggtttcattg tatatgaaac cgcttcaaaa
     8101 atcaagaaaa acttgtcatc caaatttttt tatgctagac tagtgaaaat caagctctaa
     8161 tggagggaaa agtatggaat caatatttgt gaaatttgcc cagtatccgt ctatagaaac
     8221 ggagcgttta ttgcttagac ctgtaacttt ggatgatgcg gaagcaatgt ttgactatgc
     8281 ctcggacaag ggtaatacac gttgcacttt tccaaccaat caaagcttgg aagaaaccaa
     8341 gaataacatt gctcagttct acttggctaa tcccttggga cgttggggga ttgaattaaa
     8401 agataacggc aagtttatcg gaactattga cttacacaag attgatcctg ttcttaagaa
     8461 ggcagctatt ggctacatta tcaataaaaa gtattggaat caaggattaa cgacagaagc
     8521 caatcgtgct gtgattgagc tagcttttga gaagataggg atgaataagt tgactgccct
     8581 tcacgataag gctaatcccg cgtcaggaaa ggtcatggag aaatcaggca tgcgtttttc
     8641 ccatgcagaa ccatatgctt gtatggacca gcatgaaaaa ggccgaatcg tgacaagagt
     8701 tcattatgtc ttgaccaagg aagactattt tgcaaataaa taagcagttg aaaagaaatt
     8761 ttcgactgtt ttttcttcct cttacgaata atttaagaga ggagaaaata tggaagcaat
     8821 tatcgagaaa atcaaagagt ataaaatcat cgtcatctgt actggtctgg gcttacttgt
     8881 aggaggattt ttcctgctaa aaccagctcc acaaacacct gtcaaagaga cgaatttgca
     8941 ggctgaagtc gcagctgttt ccaaggattt ggtatccgaa aaggaagtga acaaggaaga
     9001 aaaggaagaa ccccttgaac aagatctaat cacagtagat gtcaaaggtg ctgtcaaatc
     9061 gccagggatt tatgacttgc ctgtaggtag tcgagtcaat gatgctgttc agaaggctgg
     9121 tggcttgaca gagcaagcag acagcaagtc gctcaatcta gctcagaaag ttagtgatga
     9181 ggctctggtt tacgttccta ctaagggaga agaagcagtt agccaacaga ctggtttggg
     9241 gacagcttct tcaataagca aggaaaagaa ggtcaatctc aacaaggcta gtctggaaga
     9301 actcaagcag gtcaagggac tgggaggaaa acgagctcag gacattatcg accatcgtga
     9361 ggcaaatggc aagttcaaat cagtagacga gctcaagaag gtctctggca ttggtgccaa
     9421 gactatagaa aagttaaaag actatgttac agtggattaa gaatttctct attcccctaa
     9481 tttacctgag ttttctgtta ctttggcttt actacgccat tttttcaaca tcctatcttg
     9541 ctttgttggg atttgttttt ctgctagtct gtctcttttt ccaatttcct tggaaatctg
     9601 ctagcaaagt tctagtgatt tgtggaatct ttggattttg gtttgttttt caaaattggc
     9661 aacagagtca agcgagtcaa aatctggcgg attctgttga aagggtacgg attttgcctg
     9721 atactattaa ggttaatggt gatagtctat cctttcgtgg caagtctaac ggtcgtgctt
     9781 tccaagtcta ttataaactc cagtccgagg aggagaaaga agcctttcaa gctttaaccg
     9841 acctgcatga gataggacta gaagggaagc tttcggagcc agaagggcag agaaattttg
     9901 gtggctttaa ttaccaagcc tatctgaaga ctcagggaat ttaccagact ctcaatatca
     9961 aaacaatcca gtcacttcaa aagattggca gttgggatat aggagaaaac ttgtccagtt
    10021 tacgtcgaaa ggctgtggtt tggattaaga cgcactttcc agaccctatg cgcaattaca
    10081 tgacaggact cttgctggga catctggaca ccgactttga ggagatgaat gagctttatt
    10141 ccagtctagg aattatccac ctctttgccc tatctggcat gcaggtaggt tttttcatga
    10201 atggatttaa gaaacttctc ttgcgattgg gcttgaccca agaaaagttg aaatggctga
    10261 cttatccctt ttcccttatc tatgcgggac taactggatt ttcagcatcg gttattcgca
    10321 gtctcttgca aaagctactg gctcaacatg gggttaaggg cttggataat tttgccttga
    10381 cggtgcttgt cctctttatt gtcatgccaa actttttctt tacagcagga ggagtcttgt
    10441 cctgcgctta tgcttttatc ctgaccatga ccagcaaaga aggggagggg ctcaaggctg
    10501 ttgctagtga aagtctagtc atctccttgg gcatattgcc cattctatcc ttctattttg
    10561 cggaatttca accttggtct atccttttga cctttatctt ttcctttcta tttgacttga
    10621 ccttcttacc gctcttgtct attttatttg tcctttcctt tctctatcca gtcattcagc
    10681 tgaactttat ctttgaatgg ttagagggca ttattcgctt ggtctcgcag gtggcaagta
    10741 gaccgcttgt ctttggacaa cccaatacat ggcttttaat cctattgtta atttctttgg
    10801 ctttggtcta tgatttgaga aaaaacatta aaaagctaac ggtattgtgc ttattgatta
    10861 cagggctctt tctcctgacc aagcatccac tggaaaatga aatcaccatg ctggatgtgg
    10921 ggcaaggaga aagtattttc ctacgggatg taactgggaa aaccattctc atagatgtag
    10981 gtggtaaggc agaatcttat aagaaaatca aaaaatggca agaaaagatg acgaccagca
    11041 atgcccagcg aagcttgata ccctatctca aaagtcgagg agtagctaag attgaccagc
    11101 taattttgac taacacggac aaggagcatg ttggagattt gtcagagatg accaaggctt
    11161 tccatgtagg ggagattcta gtatcaaaaa acagtctgaa acagaaggaa tttgtggcag
    11221 aactacaggc gactcaaaca aaggtgcgta gtatgatagt aggggagaac ttgcccattt
    11281 ttggaagtca gttagaagtt ctatctccaa ggaaaatggg agatggagga cacgatgata
    11341 ccctagttct gtatgggaaa ttcttggata agcaatttct cttcacggga aatttggagg
    11401 agaaaggaga gaaggacttg ctgaagcact atccagactt gaaagtaaat gttttgaaag
    11461 ctagccaaca tggcaataaa aaatcatcaa gtccagcctt tctagaaaaa ctcaaaccag
    11521 agcttactct tatctcagtt ggaaagagca atcgaatgaa actcccctat caggaaacat
    11581 tgacacgact ggaaggtatc aatagcaaag tttatcgaac tgaccagcaa ggagctatac
    11641 gttttaaggg gttggatagt tggaaaatcg aaagtgttcg ataggaagga taaatgttgt
    11701 agattagtga aataaactaa aaatttgttg cataataatg ataaaaatgg tataatgaaa
    11761 acgtattcaa tattgaggat ataaaatcat taaaaatcag caaaagttgt tttattagtt
    11821 agtttataat ctattggtct tcttcagtcc agtgtatctg ctgtgacagc cactaaaagt
    11881 tacaagtatg attggaatac ggttttggaa tatagtacca actatcacga ccatcagtat
    11941 gcttggattc cggaatggtc tcgttattac agctattctg agtataaagt tggcggaggc
    12001 tggaactacg ctcgttatga ggtcataaac tactatactg gaggttatta atccttaaag
    12061 agtgagaaaa ggagggctgg atatgttaaa tcttactcat gttaccttaa aaacgcgaca
    12121 agtcatcttg caagatgcgg attttacctt taaaaagggt aggatttatg gccttcttgc
    12181 tatcaatggc tcgggaaaga cgacactatt ccgagctatg agcaagttgc ttccccttag
    12241 tagtggacac atcgcagttc ctccttcttt gttttattat gagagcgttg aatggctgga
    12301 tggaaactta agtgggatgg actaccttcg tctcataaaa aacatctgga agtcagacct
    12361 aaacttgaga gatgaaatcg cctactggga aatggctgac tatatcagtc ttcccatccg
    12421 caagtattcc ttagggatga agcaacgctt ggtgattgct atgtattttc tcagtcaggc
    12481 caaatgctgg ctcatggatg agattacaaa tggcttagac gagtattatc gacagaagtt
    12541 ttttgatagg ctagcacaaa tcgatagaca agaacagctg gttcttttaa gttcccacta
    12601 taaggaagag ttggttgata tctgcgatag agtagtaacc attcatcagg ggcagataga
    12661 agaggtttag tttatgaaag atgttagtct atttttattg aaaaaagttt tcaaaagtcg
    12721 tttaaactgg attatcttac ttttatttgc atctgtactc ggtgttacct tttatttaaa
    12781 tagtcagact gcaaactcac acagcttgga gagcaggttg gaaagtcgca ttgcagccaa
    12841 cgagagggct atcaatgaaa atgaagagaa actctcccaa atgtctgata ccagctcgga
    12901 ggaataccag tttgctaaaa ataatttaga cgtgcaaaaa aatcttttga cgcgaaagac
    12961 agaaattcta actttactaa aagaagggcg ctggaaagaa gcctactatc tgcagtggca
    13021 agatgaagag aagaattatg aatttgtatc aaatgacccg actgctagct ctggcttaaa
    13081 aatgggggtt gaccgcgaac ggaagattta ccaagccctg tatcccttga acataaaagc
    13141 acatactttg gagtttccga cccacgggat tgatcagatt gtctggattt tagaggttat
    13201 catcccaagc ttgtttgtgg ttgcgattat ttttatgcta acacaactat ttgcagaaag
    13261 atatcaaaat catctggaca cagctcactt atatcctgtt tcaaaagtga catttgcaat
    13321 atcctctctt ggagttggag tgggatatgt aactgtgctg tttatcggaa tctgtggctt
    13381 ttcttttcta gtgggaagtc tgataagtgg ttttggacag ttagattatc cctacccaat
    13441 ttatagctta gtgaatcaag aagtaactat tgggaaaata caagatgtat tatttcctgg
    13501 cttgctctta gctttcttag cctttatcgt cattgtggaa gttgtgtact tgattgctta
    13561 ctttttcaag caaaaaatgc ctgtcctctt tctttcactc attgggattg ttggcttatt
    13621 gtttggcatc caaacaattc agcctcttca aaggattgca catctgattc cctttactta
    13681 cttgcgttca gtggagattt tatctggaag attatctaag cagattgata atgtcgatct
    13741 aaattggagc atggggatgg tcttacttcc ttgcctgatt atccttttgt tagtggggat
    13801 tctatttatc gaaagctggg gaagttcacg taaaaaggaa gtttttaaag tttagttttc
    13861 ctatagggaa gctaaagaaa aactaacata gggagagaat caacttgatt ctctcttttt
    13921 gattcgaaaa ccaaaccaaa atacaaacac aaacttttca aaaaataact ttttatcttg
    13981 acaagagcta gaaaacttgg tatcatttaa aagttgagaa aagcagaagt gagagcttct
    14041 cgccttgtga cattaagttg cctggcccta cggatgaaaa gtttcgaaga aacgctatca
    14101 taacgtgcgg gcttgtatat ttacgagtcc gctattgttt ttctctaata aaacaaaaga
    14161 ggtgaaaacc atagcaaagc aagacttatt catcaatgat gagattcgtg tacgtgaagt
    14221 tcgcttgatt ggtcttgagg gagaacagct aggcatcaag ccactcagtg aagcgcaagc
    14281 tttggctgat aacgctaatg ttgacctagt attgattcaa ccccaagcca aaccgcctgt
    14341 tgcaaaaatt atggactacg gtaagttcaa atttgagtac cagaagaagc aaaaagaaca
    14401 acgtaaaaaa caaagcgttg ttactgtgaa agaagttcgt ctaagtccaa ctattgacaa
    14461 gggtgacttt gatacaaaac ttcgtaatgc acgtaagttc cttgaaaaag gaaataaagt
    14521 taaggtatct attcgcttta agggtcgtat gattacccat aaagagattg gtgcaaaagt
    14581 tttagccgag tttgctgaag caactcaaga tattgccatc atcgaacaac gtgctaaaat
    14641 ggatggacgt caaatgttca tgcaattggc gccagcgact gacaaaaaat aattgtcaga
    14701 aagttaaata aaggagaaaa aatcatgcca aaacaaaaaa cacaccgcgc atcagctaaa
    14761 cgtttcaaac gtacaggttc tggtggactt aaacgtttcc gtgcttacac ttctcaccgt
    14821 ttccacggaa aaactaagaa acaacgtcgt catcttcgta aagcatctat ggtgcattca
    14881 ggagattaca aacgtatcaa agcaatgctt actcgcttga aataatagct tgactgtaag
    14941 taaacaattc taggaaatat ttggaggaaa taaaacatgg cacgtgttaa aggtggcgtt
    15001 gtatcacgca aacgtcgtaa acgtattctt aaattagcaa aaggttacta tggagctaaa
    15061 cacatcttgt tccgtactgc aaaagaacaa gtaatgaact cttactacta tgcataccgt
    15121 gaccgtcgtc agaaaaaacg tgacttccgc aaattgtgga ttactcgtat caatgcggca
    15181 gctcgtatga acggactttc atactcacaa ttgatgcatg gtttgaaatt ggctgagatc
    15241 gaagttaacc gtaaaatgct tgctgacttg gctgttaacg atgcggtagc tttcacagct
    15301 cttgcagatg cagctaaagc aaaacttggt aaataacaac tgatgagact ggaacaggat
    15361 tgtccagtct ttttaaaaat aaaggagaaa gatatggctt caaaaatgct acacacttgc
    15421 ttacgagtag aaaatcttga aaaatcaatc gcattttata aagatgcttt tggttttaaa
    15481 gaattgcgtc gcagagattt tccagaccat gccttcacga ttgtctatct aggtcttgag
    15541 ggtgatgatt atgagttgga gttgacttat aactacgatc acggtccgta tgtggttggt
    15601 gatgggtttg cccatatcgc cctcagtaca cctgatcttg aggcacttca tcaagaacac
    15661 agtgcaaaag gctatgaagt gactgagcca aatggtctac caggaactac accaaactat
    15721 tactttgtca aagaccctga tggatataag gtcgaagtca ttcgtgaaaa ataatttcgt
    15781 gcaagttaag tagaatattc gttttctact tgactttttt tagctgaaag agtatactaa
    15841 taaaaattca acctttaagg ggagtccaga gagactcaca aggtgtcaga taaaagaata
    15901 gtgcaatttt ctagaggaga ctttttgagt gtgctctctt gtgttgtacg attttaactg
    15961 aggccttgca ctagcaaggt cttttcttta tctggtcccc ttaaaattta aggaggaaaa
    16021 gttatgaatc ccacatgtaa gaagcgtttg ggtgtcattc ggttggaaac catgaaggtg
    16081 gttgcacaag aggaaatcgc gccagcaatc tttgaattag tcctagaagg agaaatggtt
    16141 gaagccatgc gagcaggcca atttcttcat ctgcgtgtac cggacgatgc ccatctctta
    16201 cgtcgtccta tttcaatttc gtctattgac aaggcaaaca agcagtgtca cctcatttat
    16261 cggattgagg gagctgggac tgcaattttt tcaaccttaa gtcagggaga cactcttgat
    16321 gtgatggggc ctcagggaaa tggttttgac ttgtctgacc ttgatgagca gaatcaggtt
    16381 ctccttgttg gtggtgggat tggtgttcca cccttgcttg aggtggccaa ggaattgcat
    16441 gaacgtggag tgaaagtagt gacagtcctc ggttttgcta ataaggatgc tgttattttg
    16501 aaaacggaat tggctcagta tggtcaggtc tttgtaacga cagatgatgg ttcttatggc
    16561 atcaagggaa atgtttccgt tgttatcaat gatttagata gtcagtttga tgctgtttac
    16621 tcgtgtgggg ctccaggaat gatgaagtat atcaatcaaa cctttgatga tcacccaaga
    16681 gcctatttat ctctggaatc tcgtatggct tgtgggatgg gagcttgcta tgcctgtgtt
    16741 ctaaaagtac cagaaagcga gacggtcagc caacgcgtct gtgaagatgg tcctgttttc
    16801 cgcacaggaa cagttgtatt ataaggagaa aattatgact acaaatcgat tacaagtgtc
    16861 tctacctggt ttggatttga aaaatccgat tattccagca tcaggctgtt ttggctttgg
    16921 acaagagtat gccaagtact atgatttaaa ccttttaggt tctattatga tcaaggcgac
    16981 aacccttgaa ccacgttttg ggaacccaac tccaagagta gcagagacgc ctgctggtat
    17041 gctcaatgca attggcttgc aaaatcctgg tttagaggtt gttttggctg aaaagctacc
    17101 ttggctggaa agagaatatc caaatcttcc tattattgcc aatgtagctg gtttttcaaa
    17161 acaagagtat gcagctgttt ctcatgggat ttccaaggca actaatgtaa aagctatcga
    17221 gctcaatatt tcttgtccca atgttgacca ctgtaatcat ggacttttga ttggtcaaga
    17281 tccagatttg gcttatgatg tggtgaaagc agctgtggaa gcctcagaag tgccagttta
    17341 tgtcaaatta accccgagtg tgaccgatat cgttactgtc gcaaaagctg cagaagatgc
    17401 gggagcaagt ggcttgacca tgatcaatac tctggttgga atgcgcttcg acctcaaaac
    17461 cagaaaacca atcttggcca atggaacagg tggaatgtca ggtccagcag tctttccagt
    17521 agccctcaaa ctcatccgcc aagtagccca aacaacagac ctgcctatca ttggaatggg
    17581 gggagtggat tcggctgaag ctgccctaga aatgtatctg gctggggcat ctgctatcgg
    17641 agttggaaca gctaacttta ccaatcctta tgcctgccct gacatcatcg aaaatttacc
    17701 aaaagtcatg gataaatacg gtattagcag tctggaagaa ctccgtcagg aagtaaaaga
    17761 gtttctgagg taaactgcaa tcaatctgtt cttgattttt tattagtttg taatatgaat
    17821 ttaggagaat tttggtacaa taaaataaat aagaacagag gaagaaggtt aatgaagaaa
    17881 gtaagattta tttttttagc tctgctattt ttcttagcta gtccagaggg tgcaatggct
    17941 agtgatggta cttggcaagg aaaacagtat ctgaaagaag atggcagtca agcagcgaat
    18001 gagtgggttt ttgatactca ttatcaatct tggttctata taaaagcaga tgctaactat
    18061 gctgaaaatg aatggctaaa gcaaggtgac gactattttt acctcaaatc tggtggctat
    18121 atggccaaat cagaatgggt agaagacaag ggagcctttt attatcttga ccaagatgga
    18181 aagatgaaaa gaaatgcttg ggtaggaact tcctatgttg gtgcaatagg tgccaaagta
    18241 atagaagact gggtctatga ttctcaatac gatgcttggt tttatatcaa agcagatgga
    18301 cagcacgcag agaaagaatg gctccaaatt aaagggaagg actattattt caaatccggt
    18361 ggttatctac tgacaagtca gtggattaat caagcttatg tgaatgctag tggtgtcaaa
    18421 gtacagcaag gttggctttt tgacaaacaa taccaatctt ggttttacat caaagaaaat
    18481 ggaaactatg ctgataaaga atggattttc gagaatggtc actattatta tctaaaatcc
    18541 ggtggctaca tggcagccaa tgaatggatt tgggataagg aatcttggtt ttacctcaag
    18601 tttgatggca aaatggctga aaaagaatgg gtctacgatt ctcatagtca agcctggtac
    18661 tacttcaaat ccggtggtta catgacagcc aatgaatgga tttgggataa ggaatcttgg
    18721 ttttacctca aatctgatgg gaaaatagct gaaaaagaat gggtctacga ttctcatagt
    18781 caagcttggt actacttcaa atccggtggt tacatgacag ccaatgaatg gatttgggat
    18841 aaggaatctt ggttttacct caagtctgat ggcaaaatgg ctgaaaaaga atgggtctac
    18901 gattctcata gtcaagcctg gtactacttc aaatctggtg gctacatggc gaaaaatgag
    18961 acagtagatg gttatcagct tggaagcgat ggtaaatggc ttggaggaaa agctacaaat
    19021 gaaaatgctg cttactatca agtagtgcct gttacagcca atgtttatga ttcagatggt
    19081 gaaaagcttt cctatatatc gcaaggtagt gtcgtatggc tagataagga tagaaaaagt
    19141 gatgacaagc gcttggctat tactatttct ggtttgtcag gctatatgaa aacagaagat
    19201 ttacaagcgc tagatgctag taaggacttt atcccttatt atgagagtga tggccaccgt
    19261 ttttatcact atgtggctca gaatgctagt atcccagtag cttctcatct ttctgatatg
    19321 gaagtaggca agaaatatta ttcggcagat ggcctgcatt ttgatggttt taagcttgag
    19381 aatcccttcc ttttcaaaga tttaacagag gctacaaact acagtgctga agaattggat
    19441 aaggtattta gtttgctaaa cattaacaat agccttttgg agaacaaggg cgctactttt
    19501 aaggaagccg aagaacatta ccatatcaat gctctttatc tccttgccca tagtgcccta
    19561 gaaagtaact ggggaagaag taaaattgcc aaagataaga ataatttctt tggcattaca
    19621 gcctatgata cgacccctta cctttctgct aagacatttg atgatgtgga taagggaatt
    19681 ttaggtgcaa ccaagtggat taaggaaaat tatatcgata ggggaagaac tttccttgga
    19741 aacaaggctt ctggtatgaa tgtggaatat gcttcagacc cttattgggg cgaaaaaatt
    19801 gctagtgtga tgatgaaaat caatgagaag ctaggtggca aagattagta ctataagtga
    19861 atatgatttg agtgaatagt aagttaaaaa tcctgatttc aagtaaaatc aggatttttt
    19921 catggatgca atttttttgg agtctggtgt gacgcggagg gtcttttgtc ctgtgtaagt
    19981 gacaaagccg ggttttccac cagttggttt attgagtttt ttgacttcaa tcatatctac
    20041 ctgcaccaga ttcgacaggc gcccttgaga gaagtaggca gctaactctg ctgcgtctgt
    20101 cttgactgca tcagatgggt caagatttcc tgagatgaca acatggcttc caggaatgtc
    20161 cttagcatgg aaccaaagtt cctccttgcg ggccatttta aaggtcaatt cctcattttg
    20221 aagattgttt cgtccgacat agatgatggt tttgccatcg cttgctagat attgttctag
    20281 ttttttgcgt ttctggattt tctcccgttg tcttctgcgg ataaaacctg tttgaatcaa
    20341 ttcttcgcgg atttcagcga tttcttccag tccagcttgg ttgaggacgg tttctacact
    20401 ttccagatag agaatagtgg ctttggtttc ttcaatcaaa tcagtcaagt atttgacagc
    20461 ttctttgagt ttctgatacc gtttaaaata gcgttgggca ttctggttgg gagtcagagc
    20521 cttatcaagc gcaatcatga taggttggtt ggtatagtag ttgtctagga taacctggtc
    20581 ttggtcgtta ggcacttggt gtaggaaggt tgtcagcaat tctccttttt gacgaaattc
    20641 ttcagcgttg tctgtcgcca gtaactcttt ttcctgtttt ttgagtttgt gtcggttttt
    20701 ctgaagttca ttttcaacac gacgaatcag ttcactggcc tgctgtttga cgcggtcgcg
    20761 ctcagcctta tccttatagt aggtgtccaa caaatcagaa agatttgcaa aaggctctcc
    20821 cacctgattt gcaaaaggaa ctggactgaa ggaagtctca gtcaagcatg gcttggtttc
    20881 ttgattgaaa aaatttcgga aagcggaaag tttttcacta accagtatcc tttccaattc
    20941 atttgccgta tcgcgtccca gaccttgaaa gaggctttga agattttttg ctgttagttc
    21001 ttgggtttgc aggatttcaa agagcttttc atctttgata gtaaaaggat tgagagattc
    21061 tgtacttggc ggagcgatat aggtcgatcc tggaagtaag gtgcggtagc tattttgtga
    21121 aaaaccgacg tgtttgataa cttcgaggat tttatgactg cttttatcga ccagtagaat
    21181 attactgtgt ttccccataa tttcaataat caaggtagcc tggatatggt ctccaatctc
    21241 gtttttattg gaaactgtaa tttccacaat acggtcattt tccacttgct caatcgactc
    21301 aatcagggcc ccctgcaaat actttctcaa aaccatgata aaggtagaag gttgagctgg
    21361 attttcaaaa gtcgtttggg tcagctgaat gcgtccaaaa actggatggg cagaaaggag
    21421 caggcgatgg ctttggcgat tgctgcggat ttgcaagacc aactcttgtt caaaaggctg
    21481 attgattttc tggatgcgac cattcactaa ttcgcttcgc aattcctcaa ctatgtggtg
    21541 taaaaaaaat ccgtcaaatg acatcgttct ctccttgtga ttgtattcca tagtattata
    21601 tcaaaaaggt agaataaaat catggaaatg tggtataata aagccaagta aagagaaacg
    21661 agaagcacat gtatattgaa atggtagatg aaactggtca agtttcaaaa gaaatgttgc
    21721 aacaaaccca agaaattttg gaatttgcag cccaaaaatt aggaaaagaa gacaaggaga
    21781 tggcagtcac ttttgtgacc aatgagcgta gtcatgaact taatctggag taccgtgaca
    21841 ccgaccgtcc gacagatgtc atcagccttg agtataaacc agaattggaa attgcctttg
    21901 acgaagagga tttgcttgaa aatccagaat tggcagagat gatgtctgag tttgatgcct
    21961 atattgggga attgttcatc tctatcgata aggctcatga gcaggccgaa gaatatggtc
    22021 acagctttga gcgtgagatg ggcttcttgg cagtacacgg ctttttacat attaacggct
    22081 atgatcacta tactccggaa gaagaagcgg agatgttcgg tttacaagaa gaaattttga
    22141 cagcctatgg actcacaaga caataaacga aaatggaaaa atcgtgactt gatatccagt
    22201 ttagaatttg ccatcacagg gatttttact gctatcaagg aagaacgaaa tatgcgaaaa
    22261 catgcagtga cggctctagt ggtcatcctt gcaggttttg tttttcaggt gtcacgaatc
    22321 gaatggctct ttcttctatt gagtattttc ttggtagtag cctttgagat tatcaactct
    22381 gctattgaaa atgtggtgga tttggccagt cactatcact tttccatgct ggctaaaaat
    22441 gccaaggata tggcggccgg cgcggtatta gtggtttctc tttttgcagc cttaacaggc
    22501 gcattgattt ttctcccacg aatctgggat ttattatttt aaacagtaag aggaaattat
    22561 gacttttaaa tcaggctttg tagccatttt aggacgtccc aatgttggga agtcaacctt
    22621 tttaaatcac gttatggggc aaaagattgc catcatgagt gacaaggcgc agacaacgcg
    22681 caataaaatc atgggaattt acacgactga taaggagcaa attgtcttta tcgacacacc
    22741 agggattcac aaacctaaaa cagctctcgg agatttcatg gttgagtctg cctacagtac
    22801 ccttcgcgaa gtggacaccg ttcttttcat ggtgcctgct gatgaagcgc gtggtaaggg
    22861 ggacgatatg attatcgagc gtctcaaggc tgccaaggtt cctgtgattt tggtggtgaa
    22921 taaaatcgat aaggtccatc cagaccagct cttgtctcag attgatgact tccgtaatca
    22981 aatggacttt aaggaaattg ttccaatctc agcccttcag ggaaataacg tgtctcgtct
    23041 agtggatatt ttgagtgaaa atctggatga aggtttccaa tatttcccgt ctgatcaaat
    23101 cacagatcat ccagaacgtt tcttagtttc agaaatggtt cgcgagaaag tcttgcacct
    23161 aactcgtgaa gagattccgc attctgtagc agtagttgtt gactctatga aacgagacga
    23221 agagacagac aaggttcaca tccgtgcaac catcatggtc gagcgcgata gccaaaaagg
    23281 gattatcatc ggtaaaggtg gcgctatgct taagaaaatc ggtagtatgg cccgtcgtga
    23341 tatcgaactc atgctaggag acaaggtctt cctagaaacc tgggtcaagg tcaagaaaaa
    23401 ctggcgcgat aaaaagctag atttggctga ctttggctat aatgaaagag aatactaagt
    23461 agaggtaggc tcatgcctgc ttcttgtttt tacagaagga ggacttatgc ctgaattacc
    23521 tgaggttgaa accgtttgtc gtggcttaga aaaattgatt ataggaaaga agatttcgag
    23581 tatagaaatt cgctacccca agatgattaa gacggatttg gaagagtttc aaagggaatt
    23641 gcctagtcag attatcgagt caatgggacg tcgtggaaaa tatttgcttt tttatctgac
    23701 agacaaggtc ttgatttccc atttgcggat ggagggcaag tatttttact atccagacca
    23761 aggacctgaa cgcaagcatg cccatgtttt ctttcatttt gaagatggtg gcacgcttgt
    23821 ttatgaggat gttcgcaagt ttggaaccat ggaactcttg gtgcctgacc ttttagacgc
    23881 ctactttatt tctaaaaaat taggtcctga accaagcgaa caagactttg atttacaggt
    23941 ctttcaagct gcccttgcca agtccaaaaa gcctatcaaa tcccatctcc tagaccagac
    24001 cttggtagct ggacttggca atatctatgt ggatgaggtt ctctggcgag ctcaggttca
    24061 tccagctaga ccttcccaga ctttgacagc agaagaagcg actgccattc atgaccagac
    24121 cattgctgtt ttgggccagg ctgttgaaaa aggcggttcc accattcgga cctataccaa
    24181 tgcctttggg gaagatggaa gcatgcagga ttttcatcag gtctatgata agactggtca
    24241 agaatgtgta cgctgtggta ccatcattga gaaaattcaa ctaggcggac gaggcaccca
    24301 cttttgtcca aactgtcaaa ggagggactg atgagaaaaa tcatcggaat cactggggga
    24361 attgcctctg gtaagtcaac tgtgacaaat tttctaagaa agcaaggctt tcaagtagtg
    24421 gatgccgacg cagtcgtcca ccaactacag aaacctggtg gtcgtctgtt tgaggctcta
    24481 gtacagcact ttgggcaaga aatcattctt gaaaacggag aactcaatcg ccctctccta
    24541 gctagtctca tcttttcaaa tcctgaagag caaaaatggt ctaagcaaat tcaaggggag
    24601 attatccgtg aggaactggc tactttgaga gaacagttgg ctcagacaga agagattttc
    24661 ttcatggata ttcccctact ttttgagcag gactacagcg attggtttgc tgagacttgg
    24721 ttggtctatg tggaccgaga tgcccaagta gaacgcttaa tgaaaaggga ccagttgtcc
    24781 aaagatgaag ctgagtctcg tctggcagcc cagtggcctt tagaaaaaaa gaaagatttg
    24841 gccagccagg ttcttgataa taatggcaat cagaaccagc ttcttaatca agtgcatatc
    24901 cttcttgagg gaggtaggca agatgacaga gattaactgg aaggataatc tgcgtattgc
    24961 ctggtttggt aattttctga caggagccag tatttctttg gttgtacctt ttatgcccat
    25021 cttcgtggaa aatctaggtg tagggagtca gcaagtcgct ttttatgcag gcttagcaat
    25081 ttctgtctct gctatttccg cggcgctctt ttctcctatt tggggtattc ttgctgacaa
    25141 atacggccga aaacccatga tgattcgggc aggtcttgct atgactatca ctatgggagg
    25201 cttggccttt gtcccaaata tctattggtt aatctttctt cgtttactaa acggtgtatt
    25261 tgcaggtttt gttcctaatg caacggcact gatagccagt caggttccaa aggagaaatc
    25321 aggctctgcc ttaggtactt tgtctacagg cgtagttgca ggtactctaa ctggtccctt
    25381 tattggtggc tttatcgcag aattatttgg cattcgtaca gttttcttac tggttggtag
    25441 ttttctattt ttagctgcta ttttgactat ttgctttatc aaggaagatt ttcaaccagt
    25501 agccaaggaa aaggctattc caacaaagga attatttacc tcggttaaat atccctatct
    25561 tttgctcaat ctctttttaa ccagttttgt catacaattt tcagctcaat cgatcggccc
    25621 tattttggct ctttatgtac gcgacttagg gcagacagag aatcttcttt ttgtctctgg
    25681 tttgattgtg tccagtatgg gcttttccag catgatgagt gcaggagtca tgggcaagct
    25741 aggtgacaag gtgggcaatc atcgtctctt ggttgtcgcc cagttttatt cagtcatcat
    25801 ctatctcctc tgtgccaatg cctctagccc ccttcaacta ggactctatc gtttcctctt
    25861 tggcttggga accggtgcct tgattcccgg ggttaatgcc ctactcagca aaatgactcc
    25921 caaagccggc atttcgaggg tctttgcctt caatcaggta ttcttttatc tgggaggtgt
    25981 tgttggtccc atggcaggtt ctgcagtagc aggtcaattt ggctaccatg ctgtctttta
    26041 tgcgacaagc ctttgtgttg cctttagttg tctctttaac ctgattcaat ttcgaacatt
    26101 attaaaagta aaggaaatct agtgcgagta aaaattaatc tcaaatgctc ctcttgtgac
    26161 agtatcaatt acctaaccag taaaaattca aaaacccatc cagacaagat tgaggtttta
    26221 aagtattgtc ccaaggaaag aaaagtaacc ctacatcttg aatctaagta gattttatgg
    26281 taaaataata gggattttaa ggagtttgat atgtataacc tattattaac cattttatta
    26341 gtattatctg ttgtgattgt gattgcaatt ttcatgcaac caaccaaaaa ccaatccagc
    26401 aatgtatttg atgccagttc aggtgatttg tttgaacgca gtaaagctcg cggttttgaa
    26461 gctgtaatgc agcgtttgac agggatttta gtctttttct ggctagccat tgccttagca
    26521 ttgacggtat tatcaagtag ataagaaaat aatgggcagg actaggtctt tgcctctttt
    26581 tatttttaaa ggatgtttga gaaggtttta cagtaaaaga aaattaaaaa atctagaaag
    26641 aaaatatgaa agatagaata aaagaatatt tacaagacaa gggaaaggtg actgttaatg
    26701 atttggctca ggctttggga aaagacagtt ccaaggattt tcgtgagttg attaaaacct
    26761 tgtccttaat ggaaagaaag caccagattc gctttgaaga agatggcagt ctgaccttgg
    26821 aagccaagaa aaaacatgag attaccctca aggggatttt tcatgcccat aaaaatggtt
    26881 ttggttttgt cagcctagaa ggggaagagg acgacctttt tgtagggaaa aatgatgtca
    26941 actatgctat tgatggtgat accgtcgagg tagtgattaa gaaagtcgct gaccgcaata
    27001 agggaacagc agcagaagcc aaaattattg atatcctaga acacagtttg acaacagttg
    27061 tcgggcaaat cgttctggat caggaaaagc ctaagtatgc tggctatatt cgttcaaaaa
    27121 atcagaaaat cagtcaaccg atttatgtta agaaaccagc cctaaagtta gaaggaacag
    27181 aagttctcaa ggtctttatc gataaatacc caagcaagaa acatgatttc tttgtcgcga
    27241 gtgttctcga tgtagtggga cactcaacgg atgtcggaat tgatgttctt gaggtcttgg
    27301 aatcaatgga cattgtatcc gagtttccag aagctgttgt taaggaagca gaaagtgtgc
    27361 ctgatgctcc gtctcaaaag gatatggaag gtcgtctgga tctaagagat gaaattacct
    27421 ttaccattga cggtgcggat gccaaggact tggacgatgc agtgcatatc aaggctctga
    27481 aaaatggcaa tctggagctt ggagttcaca tcgcagatgt ttcttattat gtgaccgagg
    27541 ggtctgccct tgacaaggaa gcccttaacc gtgcgacttc tgtttacgtg acagaccgag
    27601 tggtgccaat gcttccagaa cgactatcaa atggcatctg ctctctcaat ccccaagttg
    27661 accgcctgac ccagtctgct attatggaga ttgataaaca tggtcgtgtg gtcaactata
    27721 ccattataca aacagttatc aagaccagtt ttcgtatgac ctatagcgat gtcaatgata
    27781 tcctagctgg cgatgaagaa aagagaaaag aatatcataa aattgtacca agtatcgaac
    27841 tcatggccaa gcttcatgaa actttagaaa acatgcgtgt gaaacgtgga gctctcaatt
    27901 ttgataccaa tgaagcgaag attttagtgg ataaacaagg taagcctgtt gatatcgttc
    27961 ttcggcagcg tggcattgcc gagcggatga ttgagtcttt tatgttgatg gctaatgaaa
    28021 cagttgccga acatttcagc aagttggatt tgccttttat ctatcgaatt cacgaggagc
    28081 ctaaggctga aaaggttcag aagtttattg attatgcttc gagttttggc ttgcgtattt
    28141 atgggactgc cagtgagatt agtcaggagg cacttcaaga catcatgcgt gctgttgagg
    28201 gagaacctta tgcagatgta ttgtccatga tgcttcttcg ctctatgcag caggctcgtt
    28261 attcggagca caatcacggc cactatggac tagctgctga ctattatact cactttacca
    28321 gtccaattcg tcgttatcca gaccttcttg ttcaccgtat gattcgggat tacggccgtt
    28381 ctaaggaaat agcagagcat tttgaacaag tgattccaga gattgcgacc cagtcttcca
    28441 accgtgaacg tcgtgccata gaagctgagc gtgaagtcga agccatgaaa aaggctgagt
    28501 atatggaaga atacgtgggt gaagagtatg atgcagttgt atcaagtatt gtcaaattcg
    28561 gtctctttgt cgaattgcca aacacagttg aaggcttgat tcacatcact aatctacctg
    28621 aattttatca tttcaatgag cgtgatttga ctcttcgtgg agaaaaatca ggtatcactt
    28681 tccgagtggg tcagcagatc cgtatcagtg ttgaaagagc ggataaaatg actggagaga
    28741 ttgatttttc attcgtacct agtgagtttg atgtgattga aaaaggcttg aaacagtcta
    28801 gtcgtagtgg cagagggcgt ggttcaaatc gtcgttcgga taagaaggaa gacaagagaa
    28861 aatcaggacg ctcaaatgat aagcgtaagc attcacaaaa agacaagaag aaaaaaggaa
    28921 agaaatcttt ttacaaggaa gtagctaaga aaggagccaa gcatggcaaa gggcgaggga
    28981 aaggtcgtcg cacaaaataa aaaggcacgc cacgactata caatcgtaga tacgctagag
    29041 gcagggatgg tcctgactgg aactgaaatc aagagtgtac gagctgctcg aattaatctc
    29101 aaggatggct ttgctcaagt gaaaaatgga gaagtttggc tgagcaatgt tcatatcgcg
    29161 ccttacgaag agggcaatat ctggaaccag gaaccagaac gtcgtcgtaa actcctgctc
    29221 cataaaaagc aaattcaaaa attggaacaa gagaccaaag ggacaggaat gaccttagtt
    29281 ccccttaagg tctatataaa agatggctac gctaagcttc ttttaggact tgccaaaggg
    29341 aagcatgact atgacaaacg ggagtctatc aaacgtcgtg agcaaaatcg agatatcgcg
    29401 cgtgtgatga aagctgttaa tcagcgataa aaagaggaat tgaaaatgga aaaattagtt
    29461 gcctataaac gcatgccttt gtggaataaa caaacaatgc ctgaagctgt tcagcaaaag
    29521 cacaatacaa aagttgggac ttgggggaaa attactgtct tgaagggagc tctcaagttt
    29581 attgaattga cagaagaagg ggaagttcta gctgaacacc tctttgaagc aggggcagac
    29641 aatccaatgg cccaacctca agcctggcac cgagtggaag ctgccacaga tgatgtggaa
    29701 tggtacttgg aattttattg taaacctgag gattattttg ctaaaaaata caatactaat
    29761 cctgttcatt cagaggtcct agaggccatg cagacagtga aacaagggaa agctttggat
    29821 ttgggttgtg gtcaggggcg taattctctt tttctagccc agcaagattt tgatgtgacg
    29881 gctgtagatc aaaatggact ggctcttgaa atcttgcaaa gcattgtgga gcaggaagat
    29941 ttggacatgc ctgttggcct ttacgatatc aattcagcta gcattggaca agaatatgat
    30001 tttatcgttt caacagttgt tctcatgttt ctacaagcgg accgcattcc agctattatt
    30061 caaaatatgc aggagaaaac cagtgttggt ggttacaacc ttatcgtttg tgccatggac
    30121 acggaggatt atccttgctc ggttaatttc ccattcacct ttaaagaagg agaactggca
    30181 gactattaca aggattggga attggttaag tacaatgaaa atccaggcca tttgcaccgt
    30241 cgcgatgaga atggcaatcg tattcaacta cgctttgcga ccttactagc taagaaaatc
    30301 aagtaaacac acatgaagat taggaatttt cctgatcttt tttctttttt acgaatgata
    30361 tagaaaagga gggaattcat gtttgttgcg agagatgcta ggggagaatt ggtaaatgtg
    30421 ttagaggata aacttgagaa gcaagcatac acctgcccag cttgtggagg ccagctccat
    30481 ttgcgtcaag gaccaagtgt acggacgcat tttgcccata aatccttaaa agactgtgat
    30541 tttttctttg aaaatgaaag tccagaacac ctggccaata aggaatccct ctatcactgg
    30601 ttgaaaaaag agacaaaggt tcaattagag tacccgcttt cagaacttaa acagattgcg
    30661 gatgtatttg taaatggcaa tctagctcta gaagttcagt gtagtccctt gcctcagaaa
    30721 gtccttaaag agcgaagtga gggctatcgt agtcagggtt accaagtact gtggttgctg
    30781 ggtcaaaaac tgtggctcaa ggagcgtttg actcgtctac agcaaggttt tctttatttc
    30841 agtcaaaaca tgggctttta tgtttgggaa ttagacaagg aaaaacaagt tttaagactc
    30901 aaatacctga tttaccagga tctccgcggt aaactccatt atcaaatcaa ggaattttcc
    30961 tatggtcaag gtagtttatt ggaaatattg cgtcttccct ataagagaca aaaaatatct
    31021 cattttacag tttctgagga caaggacatc tgtcgctata tccggcaaca actttattat
    31081 caaaatctct tttggatgaa agaacaagca gaagcctatc aaaagggaga aaatatcctg
    31141 acttatggac tgaaagaatg gtatccacaa attcgaccaa tagtgggcaa atttttccag
    31201 attgaacaag acttgactag ctattatcag cacttttata cctattacca aaaaaatcct
    31261 caaaatgatt ggcaaaagct ttatccacca gccttttatc agcaatattt cttgaaaaat
    31321 atggtagaat agaaaggatg gaggaatcta atggtattac aaagaaatga aataaatgaa
    31381 aaagatacat gggatctatc aacgatctac ccaactgacc aggcttggga agaagcctta
    31441 aaagatttaa cagaacaatt ggagacagta gcccagtatg aaggccatct cttggatagt
    31501 gcggataacc tactagaaat cactgaattt tctcttgaaa tggaacgcca gatggagaag
    31561 ctttacgttt atgctcatat gaagaatgac caggatacac gtgaagctaa gtatcaagag
    31621 tactatgcca aggccatgac actctacagc cagttagacc aagccttttc attctatgat
    31681 cctgaattta tggagattag cgaaaagcag tatgctgact ttttagaagc tcaaccaaag
    31741 ctgcaggttt atcaacacta ttttgacaag ctcttgcaag gcaaggatca cgttctttca
    31801 caacgtgaag aagaattatt ggctggagct ggagaaatct ttggttcagc aagtgaaacc
    31861 ttcgctatct tggacaatgc ggatattgtg ttcccttatg tcctagacga tgatggtaaa
    31921 gaagttcagc tatctcatgg gacttacaca cgtttgatgg agtctaaaaa acgtgaggtt
    31981 cgccgtggtg cctatcaagc tctttatgcg acttacgaac aattccaaca cacctatgcc
    32041 aaaaccttgc aaaccaatgt taaggtgcaa aattaccgtg ctaaagttcg taactacaag
    32101 agtgctcgtc atgcagccct cgcagcgaat tttgttccag aaagtgttta tgacaatttg
    32161 gtagcagcag ttcgcaagca tttgccactc ttacatcgct atcttgagct tcgttcaaaa
    32221 atcttgggga tttcagatct caagatgtac gatgtctaca caccgctttc atctgttgaa
    32281 tacagtttta cctaccaaga agccttgaaa aaagcagaag atgctttggc agtcttgggt
    32341 gaggattact tgagccgtgt taaacgtgcc ttcagcgagc gttggattga tgtttacgaa
    32401 aatcaaggca agcgttcagg tgcctactct ggtggttctt acgataccaa tgcctttatg
    32461 cttctcaact ggcaagacaa tctggacaat ctctttactc ttgttcatga aacaggtcac
    32521 agtatgcatt caagctatac tcgtgaaact cagccttatg tttacgggga ttactctatc
    32581 tttttggctg agattgcctc aactaccaat gaaaatatct tgacggagaa attattggaa
    32641 gaagtggaag acgacgcaac acgctttgct attctcaata acttcctaga tggtttccgt
    32701 ggaacagttt tccgccaaac tcaatttgct gagtttgaac acgccattca ccaagcagat
    32761 caaaatgggg aggtcttgac aagcgatttc ctaaataaac tctacgcaga cttgaaccaa
    32821 gagtattatg gtttgagtaa ggaagacaat cctgaaatcc aatacgagtg ggctcgcatt
    32881 ccacacttct actataacta ctatgtatat caatattcaa ctggctttgc ggccgcctca
    32941 gccttggctg aaaaaattgt ccatggtagt caagaagacc gtgaccgcta tatcgactac
    33001 ctcaaggcag gtaagtcgga ctatccactt aatgtcatga gaaaagctgg tgttgatatg
    33061 gagaaggaag actacctcaa cgatgccttt gcagtctttg aacgccgttt aaatgagttt
    33121 gaagcccttg ttgaaaaatt aggattggca taaaatggtt gaatcgtata gtaagaatgc
    33181 taaccataac atgcgtcgtc ctgtcgtcaa agaagaaatt gtagacttga tgcgtcagcg
    33241 tcaaaagcag gtcacaggtt tcttgaaaga attggaagac tttgcccgca aggaaaatat
    33301 tcctattatt ccccatgaaa cggttgctta tttccgtttt cttatggaaa ccatgcagcc
    33361 taaaaatatt ctggaaattg ggacggctat cggtttttca gctctcttga tggctgaaca
    33421 tgcgccaaat gctaagatta caactattga tcgtaatcca gaaatgattg gttttgccaa
    33481 ggaaaatttt gcccagtttg acagtcgcaa gcaaatcact ctcctagagg gagatgcggt
    33541 ggatgtctta tctacactga cagagtctta tgatttcgtc tttatggatt ctgccaagtc
    33601 taaatacatc gtctttctgc cagaaatcct caaacatttg gaagttggtg gtgtggttgt
    33661 cttggatgat atttttcaag gtggtgatgt tgccaaggat attatggaag tccgtcgtgg
    33721 tcagcgaacc atttatcgag gccttcaaaa attatttgat gcaaccttag acaatccaga
    33781 actcaccgca acattagtgc ctttaggaga tggtattctc atgcttcgta aaaatgtagc
    33841 agatgttcaa ctgtctgaaa gcgaatgatt ttcagaaaaa tttaagaaaa aatagtaaaa
    33901 tagatagagt aacacttatc tcaaaggagt agacatgaag aaaaaattat tggcaggtgc
    33961 catcacacta ttatcagtag caactttagc agcttgttcg aaagggtcag aaggtgcaga
    34021 ccttatcagc atgaaagggg atgtcattac agaacatcaa ttttatgagc aagtgaaaag
    34081 caacccttca gcccaacaag tcttgttaaa tatgaccatc caaaaagttt ttgaaaaaca
    34141 atatggctca gagcttgatg ataaagaggt tgatgatact attgccgaag aaaaaaaaca
    34201 atatggcgaa aactaccaac gtgtcttgtc acaagcaggt atgactcttg aaacacgtaa
    34261 agctcaaatt cgtacaagta aattagttga gttggcagtt aagaaggtag cagaagctga
    34321 attgacagat gaagcctata agaaagcctt tgatgagtac actccagatg taacggctca
    34381 aatcatccgt cttaataatg aagataaggc caaagaagtt ctcgaaaaag ccaaggcaga
    34441 aggtgctgat tttgctcaat tagccaaaga taattcaact gatgaaaaaa caaaagaaaa
    34501 tggtggagaa attacctttg attctgcttc aacagaagta cctgagcaag tcaaaaaagc
    34561 cgctttcgct ttagatgtgg atggtgtttc tgatgtgatt acagcaactg gcacacaagc
    34621 ctacagtagc caatattaca ttgtaaaact cactaagaaa acagaaaaat catctaatat
    34681 tgatgactac aaagaaaaat taaaaactgt tatcttgact caaaaacaaa atgattcaac
    34741 atttgttcaa agcattatcg gaaaagaatt gcaagcagcc aatatcaagg ttaaggacca
    34801 agccttccaa aatatcttta cccaatatat cggtggtgga gattcaagct caagcagtag
    34861 tacatcaaac gaatagtcca aatcaatgag tcagggaaaa aactcgactt caggaaaaaa
    34921 tgaagcaaac attcccacaa taaaacgcat agtacaaggt ttgtactgcc ccccaaaaaa
    34981 gttagacaat taatttatcc gaaggattta gttctgtatt gcacagagct aagtcctttt
    35041 agttttatct taattctctt attgttgtaa taatcaatat agtctataat ggctcgttcc
    35101 aattgattaa gtgatttaaa tgtttgctca tagccataaa acatttcgga ttttaaaatg
    35161 ccaaagaaag attccatcct accgttgtct tggctgtttc ctttgcgtga catagatgct
    35221 tgaattccct tactctctag gaagcgatga taagaatcgt gttgatattg ccagccttgg
    35281 tcactatgga gaatcgtatt ctcgtagtgc ttctctttga atgcctgttc caacattaac
    35341 gatcaatcaa tttaatcatg tacctaagat tag
//
LOCUS       DAVDWM010000017        34329 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVDWM010000017 DAVDWM010000000
VERSION     DAVDWM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.017"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..709)
                     /locus_tag="VUW49_001029"
     CDS             complement(<1..709)
                     /locus_tag="VUW49_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7980960.1"
                     /translation="MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSP
                     EQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKY
                     YLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEY
                     AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEE
                     GKIKKKDLDISIVSQGDD"
     gene            complement(880..1017)
                     /locus_tag="VUW49_001030"
     CDS             complement(880..1017)
                     /locus_tag="VUW49_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980961.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            1275..1394
                     /locus_tag="VUW49_001031"
     CDS             1275..1394
                     /locus_tag="VUW49_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET7980962.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(1490..2836)
                     /gene="glnA"
                     /locus_tag="VUW49_001032"
     CDS             complement(1490..2836)
                     /gene="glnA"
                     /locus_tag="VUW49_001032"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7980963.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2873..3229)
                     /gene="glnR"
                     /locus_tag="VUW49_001033"
     CDS             complement(2873..3229)
                     /gene="glnR"
                     /locus_tag="VUW49_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET7980964.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(3306..3833)
                     /locus_tag="VUW49_001034"
     CDS             complement(3306..3833)
                     /locus_tag="VUW49_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET7980965.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFIGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(3969..5165)
                     /locus_tag="VUW49_001035"
     CDS             complement(3969..5165)
                     /locus_tag="VUW49_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7980966.1"
                     /translation="MAKLTVKDVDLKGNKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(5255..10195)
                     /locus_tag="VUW49_001036"
     CDS             complement(5255..10195)
                     /locus_tag="VUW49_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET7980967.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVAIASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHIIFTAPHFTHYAFVYESAEKPQPAKPAPQNT
                     VLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            10488..12131
                     /locus_tag="VUW49_001037"
     CDS             10488..12131
                     /locus_tag="VUW49_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET7980968.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSDAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLIPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKVAEKGHKTEEAINTID
                     EQLTRYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFS
                     DAALKELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRL
                     NKGECSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            complement(12690..14297)
                     /locus_tag="VUW49_001038"
     CDS             complement(12690..14297)
                     /locus_tag="VUW49_001038"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7980969.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(14686..15273)
                     /locus_tag="VUW49_001039"
     CDS             complement(14686..15273)
                     /locus_tag="VUW49_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET7980970.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(15401..15850)
                     /locus_tag="VUW49_001040"
     CDS             complement(15401..15850)
                     /locus_tag="VUW49_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7980971.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            complement(15855..16244)
                     /locus_tag="VUW49_001041"
     CDS             complement(15855..16244)
                     /locus_tag="VUW49_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET7980972.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(16272..16898)
                     /locus_tag="VUW49_001042"
     CDS             complement(16272..16898)
                     /locus_tag="VUW49_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7980973.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16912..17475)
                     /locus_tag="VUW49_001043"
     CDS             complement(16912..17475)
                     /locus_tag="VUW49_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7980974.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(17640..17874)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17928..18431)
                     /locus_tag="VUW49_001044"
     CDS             complement(17928..18431)
                     /locus_tag="VUW49_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET7980975.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(18624..19454)
                     /locus_tag="VUW49_001045"
     CDS             complement(18624..19454)
                     /locus_tag="VUW49_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET7980976.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(19439..21121)
                     /locus_tag="VUW49_001046"
     CDS             complement(19439..21121)
                     /locus_tag="VUW49_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7980977.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(21223..21435)
                     /locus_tag="VUW49_001047"
     CDS             complement(21223..21435)
                     /locus_tag="VUW49_001047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980978.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDETDEVSNHTYGKAKLTWFEEI
                     FEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(21442..21990)
                     /locus_tag="VUW49_001048"
     CDS             complement(21442..21990)
                     /locus_tag="VUW49_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET7980979.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(22005..22853)
                     /locus_tag="VUW49_001049"
     CDS             complement(22005..22853)
                     /locus_tag="VUW49_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET7980980.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRVGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            23041..24390
                     /gene="trkA"
                     /locus_tag="VUW49_001050"
     CDS             23041..24390
                     /gene="trkA"
                     /locus_tag="VUW49_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET7980981.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCHIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            24394..25833
                     /locus_tag="VUW49_001051"
     CDS             24394..25833
                     /locus_tag="VUW49_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET7980982.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLITLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25958..26416)
                     /locus_tag="VUW49_001052"
     CDS             complement(25958..26416)
                     /locus_tag="VUW49_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
                     /protein_id="HET7980983.1"
                     /translation="MEVVLFSYCLNTRYLLQVVDIASSNDIILEEDTSEIMPGEIDVI
                     KSKELKVLVLCAGSGTSAQLANAINEGTQLAEVRVIANSGAYGAHYDIMGVYDLIILA
                     PQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            27140..27310
                     /locus_tag="VUW49_001053"
     CDS             27140..27310
                     /locus_tag="VUW49_001053"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7980984.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            27922..28206
                     /locus_tag="VUW49_001054"
     CDS             27922..28206
                     /locus_tag="VUW49_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980985.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            28199..28447
                     /locus_tag="VUW49_001055"
     CDS             28199..28447
                     /locus_tag="VUW49_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980986.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            28543..28761
                     /locus_tag="VUW49_001056"
     CDS             28543..28761
                     /locus_tag="VUW49_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980987.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(29128..31452)
                     /gene="pflB"
                     /locus_tag="VUW49_001057"
     CDS             complement(29128..31452)
                     /gene="pflB"
                     /locus_tag="VUW49_001057"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET7980988.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            31846..32907
                     /gene="dinB"
                     /locus_tag="VUW49_001058"
     CDS             31846..32907
                     /gene="dinB"
                     /locus_tag="VUW49_001058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7980989.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(33054..33899)
                     /locus_tag="VUW49_001059"
     CDS             complement(33054..33899)
                     /locus_tag="VUW49_001059"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7980990.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            34154..>34329
                     /locus_tag="VUW49_001060"
     CDS             34154..>34329
                     /locus_tag="VUW49_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7980991.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     IRQLNQDCLIALEDT"
BASE COUNT        10205 a         7318 c         6299 g        10507 t
ORIGIN      
        1 tatcatctcc ttgggaaaca atagaaatgt ccaaatcttt ctttttaatc ttgccttctt
       61 caaagagttt ttgtttttct gctcgtattt tttcaagtaa aacttcgact gattcatcat
      121 ttgggtcttg ttcaactaat tttccttgca tagcatattg aagaatagat ttttttagtt
      181 tatctggaaa ttctttatct agctgttcta gtctattata actttcagca tattcatcta
      241 ctttttctaa agctgattcg attgcttcta ctattcgttg ttgttcggat agtgggggga
      301 gagcaattaa taatagatta aaattataat cattgattgc aggataactt gttccagtag
      361 atttattatt aacacgattg ataaaattat ctgataataa ataatatttc aaatatgttt
      421 cgttaagtaa agtatccaaa acaataaatg ctgtactagc tatcaaatac tctttaagtt
      481 ctctaactac agcaatattt tttagatatg gtctaactgt tgaaaataag acactattct
      541 gcgaaactaa ttttctagca cgggaaggcg cttgttcagg tgaaagatat tgtagatttt
      601 tgtagttgat tatgttcttt tttctatcaa tactagacgt atctatatac ctaaaggatt
      661 tctctggctt attttgccca aaattccaat aaattgattt tatcctcacc cactcccaag
      721 tatcaggaat atcataagga acatcaattt cttgagtact tccatcagca aacttcccat
      781 aatgtttctt atgtgcttca agtatataaa aaggcgtaaa aatacgccta tagataatgg
      841 ggttgaaata ggtttattgt tgatgagatt gtagataatt caatttttta cttccaatcg
      901 aatattcaaa acctccacct tttctgcctg taattgttca tcataaaatt caatatcttc
      961 aggattttcc ccttggcaac ctcggcagaa atattcttcc gctcgatcag gattcaaaaa
     1021 tcgacaagca caaacaaaac agtcgccatc atcatttatt gagataatat agtagattga
     1081 aataagatgt aaacaaatcg attaggaaag ttaaattagt ttctagaaat ttttagcaga
     1141 tgtagtgtac tattctagtc tcaatttact atggcttcaa atatatcttt cgaaaaaata
     1201 tttacagatg tgtaattttg aagcttgcaa aagttagtaa acttgtagat ttcgatttga
     1261 agtaacttgt tttcttgccc gatattgttt ttgaaattga atttttccat agtgactcct
     1321 taattttctt ctacacgtct gatgataaat ctaattcgca aaagagtcaa gaggattttt
     1381 cgaaaaataa atagcgaccg aaatcgctat tttaagggtt ataggtattt gatggcttag
     1441 actgctgtgt gactgtttac ccacaggcaa tctttcttct atattagtat tagtaaaggt
     1501 ctaaataatt atcaatttcc cattgtgaaa cgaaggttgc ataacttgcc cattcgattc
     1561 gtttggcttc aaggaagcta gtatagatgt gatctccgag agcagcttta accacttcat
     1621 cttctgtcaa agctttcaaa gcgttgtgaa gagttgatgg aaggtctgta ataccagctt
     1681 ccttgcgctc ttctgctgtc atgatgtaga tattttcttc gataggagct ggtgcttcga
     1741 ttttattttc aataccatac aaaccaactt ccaaaagaac agccatagca acgtaagggt
     1801 tcgccattgg atccactgaa cgcaactcaa gacgagttcc cataccacgt gaagcaggta
     1861 cgcgcacaag tggcgaacgg ttacgaccag cccaagcaat gtaaacaggc gcttcataac
     1921 ctggaaccaa acgtttgtat gagttaactg ttgggttcat gatagcagta tagttgtaag
     1981 catgcttgat caaaccgcct aggaaatggt aagctgtttc tgacaactgc attccttttg
     2041 gatcatttgg atcaaagaag gcgttatttc cttctgcatc aaacaaggac atattacagt
     2101 gcatacctga tccagcaata ccaaattttg gcttcgccat aaatgttgcg taaagtccgt
     2161 gtttgcgagc aatggtttta acaacaagct taaagatttg aatcttatca caagcacgga
     2221 gaacttcatc gtacttaaag tcaatctcat gctgtccaac cgcaacctcg tggtgactcg
     2281 cttctacttc aaatcccatt ttggtcaaga cattcacaat ctcacgacgt gtgttgtccg
     2341 caaggtcagt aggtgccaaa tcaaagtagc cacccttgtc attcacttca agtgttgggt
     2401 ccccattttc atccaactta aataggaaga attctggctc tggaccaagg ttgaaggatt
     2461 tgaatccaac ttcttccatg tgacgaagag ctcgtttcaa attaccacga gggtcacccg
     2521 caaatggttc accttctgtt gtatagacat cacagatcag acctgcaaca cttccatttt
     2581 catctcccca agggaagact gtccatgtat ccaagtccgg gtacaagtac atatccgact
     2641 cattgatacg tacaaaacct tcaatagaag atccatcaaa cataaccttg ttcgacaaga
     2701 ccttatctaa ctgttcatct gtagcaggaa tttcgacgtt tttcatggtt cccaaaatat
     2761 ctgagaacat aagacgaata aaggtaacat ttttttcctt gacttcacga cgaatatctg
     2821 cagctgtgat tggcataagt tttctcctta atctatgact acttgcggtt gcctaaccgc
     2881 gaccaaaagg tgactgtact gaagcaaaac gcccctgttg gaggagttca ttgtgaagtg
     2941 cacgacgtac ttcagtctga ctaaccgctt tcttggattt cgcttcacgt tcagcatatt
     3001 ttttcttaat ggcagcgata ttataacctt cagagatata atctttgatt tcaagcagac
     3061 gatccatgtc attcaaggaa tacatgcgac gatttccttc gtttcgatcg ggcttgatca
     3121 actcttgatc ttcataataa cgaatctgac gcgccgatag atcggtcaac ttcataacac
     3181 tgccaatagg aaaaacagcc atatttcggc gaaattcttt ttccttcatt tacaatttcc
     3241 ttctttctgt ctattatagt ctaaaaaaag acaaacgtca attgataatg ttataaaatg
     3301 taacattatt tttctttttt ctctaaaaag agacgaatac gatcaatatc gtaatttacg
     3361 ataattgcga caaaaactcc tataaacgtt tctgatacac gcacaaacac gtacgaaatt
     3421 gtctcaccac ttggaattga tagggtaatg attaacatag ctgctacacc accaataacc
     3481 cctgctttgt tattcatggc tacatttgtc ataatggtta acatggtgca gattggaaca
     3541 actaccaagg tcacccaaaa ggcttcgtgg aaaaaggtat ttaataagaa gaagaccaag
     3601 gcatagagtc caccgatact atttcctaga atacgcgaag tcccaaaatg aacactctca
     3661 tcaaaactct ccctcaggct aaaaacggct gtcaaagcac caatttgaag acctttccag
     3721 ccaaaaaagc caaaaatcaa gagaactaga aaaacagcaa tacctgtttt aaaggttcgc
     3781 ataccaagtt tgaactggga tttatcgaat ttatattttt taaaataact cataatctca
     3841 actttctatt tccattttat cataaatcgg tgatttttat gagtaatagt tgagaggaag
     3901 cgtttttatt ttaagcaaaa gaaaagagga actctcatcc ctctcttctt tgatttgttt
     3961 ataaaatctt atttttctgt caaggctgca agtcctggaa gaaccttacc ttcaagaagt
     4021 tccattgatg ctccaccacc cgtactaatc catgagaact tgtctgcacg gccaaggtta
     4081 atcgctgcgg cagctgagtc accaccaccg atgattgatt taactcctgg ttgtttcacg
     4141 atagcgtcca tcacaccaat tgtaccagct tggaaatctg ggttttcaaa tacacccata
     4201 ggtccgttcc atacaactgt tttggcacca gtcaaagctt cgtcaaattt ggcgatagat
     4261 tttggaccga tgtcaagacc aaggaagcct tcagaaactg cttcaccttc agtgtcacgc
     4321 acttcagtgt aaccagcaaa tgcgttagct tcttttgagt caactggcaa gatcaattta
     4381 ccatttgctt tttcaagaag agctttcgca acatccaatt tgtcttcttc tacaagtgag
     4441 ttaccgattt cgataccttg tgctttgtag aatgtgtaag tcatcccacc accgataagg
     4501 actttatcag ctttttcaag caagttttcg ataacaccga tcttgtctga aacttttgaa
     4561 ccaccaagga tagccacgaa tggacgttct ggagtttcaa ctgcttcttg gatgtaggca
     4621 atttcgtttt caagaaggaa accagcaact gctttttcaa cgtttgctga gataccaacg
     4681 ttagatgcgt gtgcacggtg agctgtaccg aatgcatcgt ttacgaagat accatctcca
     4741 agtgatgccc agtatttacc aagttcagga tcgtttttag attctttctt gccgtcaaca
     4801 tcttcgtaac gagtgttttc aaccaagaga acttgtccat cttcaagagc gttgattgcc
     4861 gcttccaatt cagcaccacg agtgacacct gggaaaacaa catcttgacc aagttttgct
     4921 gccaagtcag ctgctacagg agcaagtgat ttaccagctt tatcagcttc ttctttcaca
     4981 cgtccaaggt gagagaaaag aattgcacgt ccaccttgtt cgatgatgta cttaatagtt
     5041 ggaagagctg ctgtgatacg gttatcgtta gtgattacgc catctttcaa tggtacgttg
     5101 aagtcaacac gaacgaggac tttgttacct ttcaagtcaa cgtctttaac agtaagtttt
     5161 gccatgttac aaaaactcct ttatttattt tattcgaaac tattatatca cactttggaa
     5221 atataaggaa agatttcaca agatttttcg atatttaatc tttatcttct cttttccgtc
     5281 tcaaggtcaa ggctagacta gcaagaccaa ggctggctaa gcctgctatt agggcttcat
     5341 tagcatcagc tgttcctgta tttggcaatt gtttgcttgc ttcttctgat ttaactgctg
     5401 tattttgagc tggtttttcc tgtgtagcta ctactgctgg tactgttttt acttttgttc
     5461 caatttcaac aatttctgga atcgcttctt ggatgacttc tgttgaacga agacgacgct
     5521 gaccgttttc atcaacttca aagacatgtc ttaacagtcc atctcgtccc tgtatgatga
     5581 ctcgttgttc cccaactagc atctcagtat tttcatgctc ttgacgatga aaggcaacct
     5641 tttcctcttg aacttccaat ttaggagcct tttgttgatc agaagtcggt tgataagtag
     5701 gttttggaag gactgtgttt tgtggtgctg gtttagcagg ttgtggtttt tcagcagatt
     5761 cataaacaaa ggcataatga gtaaagtgag gtgctgtaaa gataatatga ctatccgttt
     5821 gttcaaaagc caattctact gcctctttgc cttcaggtaa gaaaaatact ttcttaactt
     5881 tcttatcttt ttcaattggg attttcacaa tagaagcata agagagatca acatcttgac
     5941 ctttttcatc caaaccttct atttcaaaga catgagcatc ttctccagca aagtatttct
     6001 tctcttcagc acttgcttga acacgctcta ctttcaaacc tttgatgaca gtcttctctt
     6061 tatttgagaa gtgtacttca acacctgttt tactatcaga aaagactgct ggcttgtctt
     6121 ctttcaataa agcaaccaga tcttttagac tctgtttcgc tgttgtaagc tcttctggac
     6181 tcaaatctac tttttcaacc aaggccttgt ttttctcaag aagcggagct agggcagaac
     6241 ctgaaggatg gtttggtttg gcagccaaga gttcttgaac ctcacctgtt aagcgatcaa
     6301 gctctgtctt ttcttccgta aatttctctt gaccattcag agctttatga gcttctgtca
     6361 atttattaag tcggtcattg acttgatctt gggtgctagt ttcatggtct ttcaacactt
     6421 gttcggccgc ttcaattgct ttcaagaagg cttcttttac ttctggtgaa gcaaagttat
     6481 agaccacctt gttcttagca gcttcaactt ctgctaattt ttgtttgagg tactcatcgt
     6541 tcaatgctgc ttttggagat accaaaacat caaagctaac ttcatggcct tggtaatgaa
     6601 gcgtcaaggt ttgacgacca gtcttttgag catcgtaacc agaaatttca actccctcat
     6661 cagtaaagga atgttcttcc atggtgtcat tgctataagc cactgcaaag cgtccttcag
     6721 acaagtctaa gctatcacca actaggtaat cttttttcgg ttcctgactt acttcaattc
     6781 ccaaaatagt tttcggactt gcttcgtctt ggccagtgac agtcactgac aaattagcat
     6841 ttaccggttg tcccaaatat tggagagtaa gattctgttc tcccttatga tgcgtatcaa
     6901 aacctgatac tgatacacct gcgtgagtta ggcgaatgag ttcgtcctca gttcctcctt
     6961 catactgaac tcgaagaaca ccacctctaa ggtccaaatc ctccccttcg gcgtagcttg
     7021 ttttcttagg tcctgtttct aggctgactg acttgattct tctgtcatct tttggaacgg
     7081 aaactgctag gacattacta atttccttgc ctggcaactg ggtacgataa taaagaagac
     7141 cagattgatt tgtcaaatcc aatggtgcac ttgctaggtc tcctccaact tcgctcttca
     7201 aggttgcgag cggagtttga gaatttggat tatcataaac ggtaatagtt gctccagccg
     7261 gtacatctgc aaagccaact tgtaccttgt tattgcctag agaacgggct gcagccttgg
     7321 ccatcggaat attgacactc tcagtatcaa gcttttcata cattttccag ttatagatac
     7381 gaatagcctt ccatggagtt ccattgtcag aagtgacaac attcaagcgc cagtcttgag
     7441 cagtgattgg tttatcaaga gtgatatctg tcacgtgtgc tttgttacca cggacttcct
     7501 tagctagctt ccactcacca tctgcatctt tataataaag gtcaaagtct ttagtgttca
     7561 tcaagccatc gttaacagac tcaccaccag ctcctgcatg atccatgacc catctaacaa
     7621 cggtacgtgg cttggtcaaa cgaatatcca cactaccact caactgagct gaagaccatt
     7681 tatctgacaa gctagtaatg gtaccgttca acataccttc aataccttct ccaccttcag
     7741 tcttagggaa agtactatca ataactgttg cacctggaac gatattttca gctagtggtt
     7801 ttggtaggct ggtatctttt acagtcatac cccaatcaaa ggttgtggtt gcagcttctg
     7861 aacgaactcc atttttaccg actgctacaa ccttcagttc ttgagttgta ccctgagcac
     7921 ttgctgagcg gctaactttt ggtagataaa tagttgtaga agatgagcca gttagtaatt
     7981 tccagctgtc tccatctttt tcataaactt catagaaatc tgcatccttg ttgcctttaa
     8041 attgcacaac tgcttccgct tcttgggcat ttttaagaga ttgtttcact acagaaaagc
     8101 ttgtcggcga ttgtggctct tggtgattgt ccgagatagt taattgtccg aggttgaact
     8161 gataatcttt tacagcacct tcatgctcga aaaatagttt gactgcatag atggttttac
     8221 ctgctagtga gctgagatca aattcttcat ttgtccagtt gtcagaaagg gttagctctt
     8281 tccatgcatc tgcatcatcg aatttgtagt ctggagttgt agagaatgcc atataaactt
     8341 tagaaccttt tcctcccttg tgggcaacac gaagtttggt cttctcagtt acttctaact
     8401 tagtagaata aagtctcaca tcctgatctg tcttaccggc tacatcacca gagaatttaa
     8461 gggaatttcc gccattatag gcatctgtaa aatcatattc tgcacgaagt ttttcccctg
     8521 ttgaagtctg ccaccagcgc catgttggaa gaacacctga tactgaacga taattccact
     8581 cagaatcctt agaaacctta ccatctacga accatttttt accatgacct gtattaaaag
     8641 aagtagtaaa agtattacct accgctggcg tacggtccgc aactaggtta gcaataccat
     8701 accaatcttt gtcacctggt ttttggccag tagggtctcc ttgataacct gtaaagaaga
     8761 tatcttcatt tttatgataa tcttcaccag tttttcctaa acttgtaatg gtatctgggg
     8821 caaataaacc aagagaaagg cgcaatttcc cattttcgtc taaaatgtca ttccacttaa
     8881 cctttgtctt gtaggaacca ccctgttgca attccaaacc tgcaaataca tcataaggat
     8941 tacgaccaat ccagttggca gttgcaatag tgtaatcatt tttagcctta tcccagttaa
     9001 agttagcaaa gaagttatct gccggaacct tatctccttc tggttgcatg aattggtagt
     9061 tgtattctcc caaaccatct tgatggtaac gtccatagtt ataggtcatg gcatcgtacc
     9121 aagaatactt gattggatgg tttaccttag cagcatattc cttgctatag agcataaact
     9181 ggcgcatctt ttctccaaga ggtttaacca aatctccagt tgtttcttgg ttgatgaaat
     9241 agccatcata gccataatac ttggccatgt ctaccaattt acgggcaatt gggaagctac
     9301 catctgcgtc ttgcttcaaa gcttcagcaa atctttcttg atctgcaata ctattagacc
     9361 agttgaagaa gagtgtaccg tatacaggaa ccccgttacg gtgacctgca tcaataacgt
     9421 caggagttgg tacgagacct tcccagaaga ccattgaatc tagatattgc caatagtcaa
     9481 aagcataggc cttgaactct tctccaccaa cagaagcatg gtcttttgct ttagaattgg
     9541 tgtttgataa ggcttgaact tttgcttcct tgctagcttt ttcattgact aaatgacctg
     9601 tccgacgtga agcgaggaca acagatccgc ggttaatagc atcatcttca cgagcacctg
     9661 gttcccattt taacaattct tcccaagaat taaatttgat ttcctttggt tttaatgttt
     9721 tttctgtatt tttagggaca tctggctgga cttctttttc agtcttatta gcttcggctg
     9781 ctggcttaga ctcatcccct tggttagttg cttcgggctt tgcttctgtt tccttgtcag
     9841 acttaggttt tgcttctgca gatggtgttt ctgcaaccac ctcacttgct ttttcctctg
     9901 gtttagctgc gctagctgct tctgcagttt cagatgctat cgctactcct tctgcctttt
     9961 ctagcttgtt ttctctagta tgttcccctt cattctgctt atcagcttca ttggtcgaat
    10021 gctcactctc tcctgaaaca agctctgtat tagctccgct attttcagga actgcagttt
    10081 cttcagccaa ggctggacca gcaaataaaa cagcaccaat catcagcgag caggctccta
    10141 ctgataactt acgaatactg taacgacaac gtctttcaaa aaatggattc ttcattttct
    10201 cctcctaaaa gattgatttt gttgtttcaa agaaagcgct ttatattctg tagtttttta
    10261 tcaaactcta gaaccatgat aatcacattt ttatcctaaa acatttttag acaaaaaaca
    10321 ataactgttt gcacaaatct aaaataaggt taaaaactct taaaaataat acacttatca
    10381 attataattt tcttttacaa acttgacatt aacacttgtt agatttactt ttatccttta
    10441 aaatagaata ggagatattc cgcgattatt tttcggagga aaaagaaatg tccattaatt
    10501 ggcaagaaat tttatttcac tttttaggtg gtctggggct attcttatat agcatcaaga
    10561 ccatgggaga cggtttgcaa caagctgctg gagatcgcct tcgtttttac attgacaaat
    10621 atactagtaa tcctttcttt ggagttctgg ttggtattgg gatgactgct ctaattcagt
    10681 ctagttctgg tgtaacagtt attacagtcg gcctggtcag tgccggtctc ttaaccttac
    10741 gtcaggctat cgggattgtc atgggtgcta atattgggac aactgtcaca tcctttctca
    10801 tcggttttaa attaggtaac tatgccctac ctatgctctt tatcggtgcc gtctgtctct
    10861 tttttacgaa aaatcggaca gtcaataata tcggacgcat cctctttggt gtcggtggta
    10921 tcttttttgc cctcaatctc atgagcgacg caatggctcc actcaaggat ttacaggtct
    10981 ttaaggacta tatgattgag ctaagtaaga atcctgtttt gggtgtcttt gtcggtactg
    11041 gcttgacctt gctaattcaa gcttcttcgg ctaccattgg gattttacaa aacctctacg
    11101 ccggcaatct aattgaccta cagggagctt tgccagttct atttggtgac aatatcggga
    11161 caaccattac agccatcatt gcctctttag gggctaatat tgcagctaaa cgggtagcag
    11221 gagctcatgt tgccttcaac gttatcggaa cagtcgtctg cgttattttt ctaattcctt
    11281 ttactgtcct gattcattgg tttgaagcta cgctaaatct agcaccggaa atgaccatcg
    11341 cctttgctca cggaaccttt aatattacca acaccattgt ccaatttcca tttatcggag
    11401 ctctggctta ctttgtaacc aagattattc ctggagagga cgaggttgtc aaatacgaac
    11461 ccttatatct tgatgaacat ttcatcaaac aggccccatc tatcgctcta ggaaatgcta
    11521 agaaagagct cttgcactta ggaaactacg ctgctaaagc ctttgacctt tcctataagt
    11581 acatcattga cttggatgaa aaagttgctg aaaaagttgc tgaaaaaggg cataaaaccg
    11641 aagaagcaat taacaccatc gatgagcaat taacacgtta tctcattgcc ctttcaagcg
    11701 aagctctcag ccaaaaagaa agtgaagtgc ttaccaatat ccttgattcc tcccgtgatt
    11761 tggaacggat tggagaccac acggaggctc tactcaatct gactgactat cttcaacgga
    11821 aaaatgttga attctctgat gccgccttga aagaattaga ggaagtttac cgccaaacta
    11881 gtgactttat caaagatgct ctggatagtg tggaaaacaa tgatattgaa aaagcacgca
    11941 gtcttgtaga acgtcatgaa gcaatcaata agatagaacg tgttctcaga aaaacccaca
    12001 tcaaacgcct caacaaaggc gaatgttcaa cacaagctgg ggtcaacttt atcgacatca
    12061 tctcacacta cactcgtgta tcagaccacg ctatgaacct tgctgaaaag gtttttgcag
    12121 aacaaatcta agaaccaaga agctatccat cataattgga tggcttttta ctttttccta
    12181 agcaagacta ggctgaatga aactgaaaga gtattctgca gatatatagt atattgaaac
    12241 tagaatagta cacctctatt tctaaaacat tgttagaaat cgatttgact gtcctgatcg
    12301 atttttcctg ttattatttc attttattat agtggattaa attaaaaaag tatagtatat
    12361 tgaatctata acagtacatc ttgactgcta aaatatttct ataaattaat ttgactttcc
    12421 tgatagagat gttcacatct tatttcaatt cactatacaa tccagttcct aaaactttgc
    12481 tagaaattga tttggctttc cgaattaatt tgttcagatt ttatttcatt tcaatatact
    12541 gataaatcca ttcactagca cagggcaaga gggagtcgct attaatttca acggtagcag
    12601 gtacatacag aggactccta cctcagatat actgcaatat cattgtatgc aaaaaacctc
    12661 tgagattttt ctcaaaggtt ctgattttgc taattgctgt tctcaaccgc tgcagtaaca
    12721 aaggcagtgt agagttcttc tggtcggttt ggacggcttg acagttcagg gtgatactga
    12781 caagctacaa agaatttatt ttcaggaatt tccacgattt ctaccaaacg attgtctgga
    12841 gaaactcctg aaaagacaaa acctgctgcc tcaaactgct cacggaaggc attattaaac
    12901 tcataacggt gacggtgacg gcgttgcacc acttcttgat tgtgataagc agcagcagcc
    12961 ttagagccac gtttcaactt agacggataa agtcccaaac gaagggttcc acccatatcc
    13021 tcaatatcaa tctgatcacg catgatatca atgatagggt attttgtttc tggtgcaagc
    13081 tctgcagaat tggcaccttc aagacctaaa acgtgacgag caaactcgat acatgtcaac
    13141 tgcattccca agcagactcc caacattgga acatcatttt cacgcgcata gcggatggct
    13201 tggattttcc cttctgtacc acgttgacca aaaccacctg gtacgatgat cccgtccgca
    13261 tcagacaaga gttctgctac attctctgct gtcacatcat tggcattgac ccaattgatt
    13321 ttaacttcta catcattgac atagccagag tgtttcaagg cttcgaccac tgagatatag
    13381 gcatcttgca actccacata cttaccaaca agggaaatct taacttgttt cttgaggttc
    13441 atgaccttgt ccaccatggc tgaccattct gtcatatccg ctgctggtgc gtctaatttc
    13501 aaatgatcac aaacaatttg gtccatccct tgtgcctgca agttcagtgg aatttggtaa
    13561 aggtgttcaa catccaacga ttcgataacg gcttctggtg ccacatcaca gaactgggcc
    13621 agtttatttt taattccttg accagctggc tcttctgtac gaataaccaa catatttggt
    13681 tgaattccca agccacgcaa ttctttgaca gagtgttggg ttggtttggt tttcatttca
    13741 ccagcagcct tgaggtaagg aagcaaggtt gtatggatat acatgacatt atccgcaccc
    13801 acatctgcct tcatctgacg aagagcctct aggaatggca aggactcgat atctcctact
    13861 gttccaccaa cctctgtgat aatgacatca gagtcggtcg ttagagcggc acgcttgatt
    13921 ttttctttca aagcatctgt gatatgagga atgacttgaa cagttgcccc aaggtattct
    13981 ccacggcgtt ctttacgaag aacttcactg taaattttcc cagttgtcac gttggaatat
    14041 ttgttgagat tgatatcgat gaaacgttca tagtgaccca agtccaaatc tgtctcagct
    14101 ccgtcatctg tcacaaaaac ttccccgtgc tggtaaggac tcatggttcc cggatcaata
    14161 ttgatataag ggtcaaactt ttgaatggtt actttgagac cacgattttt caagagacgg
    14221 cctagactcg ctgccacaat ccctttccca atagacgata ccacaccacc agttacaaaa
    14281 atatatttcg tagacataga ttcctctttc taaaatgctc aaggtcttgt taactacgag
    14341 gggtatagaa aacaagaccc tactaataag aataatctgg gacgactgca ccagattcac
    14401 aactaatact cttcgaaaat ctcttcaaac cgcgtcaacg tcgccttgcc gtagatatgt
    14461 gttcctgact ttgtcagtct tatctacaac ctcaaaacag tgttttgagc agcctacggc
    14521 tagtttccta gcttgctctt tgattttcat tgagtataaa atacaagaag ataacttctt
    14581 gaaaaaacaa aaatagctcc ctaagaacta gggagccccg acctctaaaa gaggtgccca
    14641 aacaatatga tacctaaaaa taggataatt gtcaatagct aactcttatt cctcgctggt
    14701 ttcagcgtcg tcatctgaaa actcgtcgtc gtcttcgttg agatccacat cttctcccaa
    14761 gtcatctgga gcgatttcgt tgatttctgc atcataagct tccacttcat ttttttcatc
    14821 atctggattt tcatcatcgt atgaaagagc tggatctgct tcgtatgcat cttcgtcttc
    14881 tggatcatct gcattgtagt caatggcatc tgaatcacca tccataaagg cattgacacg
    14941 ttttttctta gcttttggtg ctacttcatc gtcgtcattt tcttcaagag cgatgatttc
    15001 ttcgtcgatt tcgtccacac cataccatga acgaagaccc catttgttgt ccccaagtga
    15061 gatgaagcta ccgtcaaagt tcaactctgt gtagaacaaa ggcaaagctt cgcggatatc
    15121 gctgtttgat gttccaaggt agttttgaat ttcgtttaca agatcgctaa aatgcatctc
    15181 atgatcgcga ccacgaagtt ccaagatagc acgcgctacc tcaatcatag atagttcact
    15241 tttttcttgc ccagcaaata cttctaattc caaagcgttt ctcctcattt atactactat
    15301 cgccagagcg aacagactct gacctcattt tatcatttac tctttatttt acgataattt
    15361 tgcagaatag tcaaaggtta agggggagaa aatggcagga ttagactaat tccaatataa
    15421 aactcattcc tttttctgtt gctccatttt ccacaaatcc aagcgacttg aaacacctcc
    15481 tagaagcatg attgtaggtg tagatttcct tgactctcaa ttctttccat ccttttactc
    15541 gagccaattc aatcaaagca cttagaatct tttttccaag tcctcgatgt tggtaagcgg
    15601 aattcccaat cacaatgggg agattatcct gagatagtgt aatatcccca attggaaacc
    15661 attctccctt ctccttgact tcaatccaaa aaagctcacc atgccgatcc aaataggaat
    15721 acatggcttc caaggtcgct tgactgtaag gaagcttcac cccatctacg aggtaaacca
    15781 agttcacatc ctgataccaa gcaaaagctt cctcacaatg attgacctta taataaggaa
    15841 caaaacacaa tgcactacct tttcaacaaa agagttgctg cttgattaaa accatcacac
    15901 cagttatacc attttgcttc atacccatct tgagctagga tacgatcttc taaatcaaaa
    15961 acagagtaaa tctttctttc ctcgcaagct tgcgcataga gatgatatag ttcatcacca
    16021 ccatctctat cccactcagc agaaatcgta tcccgatctg ccaataaagt ctgataagcc
    16081 ctgtgatgcc catctgtaat cagcaaacaa tctccaaagg caagaatact gattggatcg
    16141 acttggattg tttctgccga ctggtaaagc atctgaatat cttgcaactt cttttctgat
    16201 aaatatagtt gagtcggatg aagatctgct atgttgactt tcatttcttt ctcctcaagg
    16261 gaattcgata ctcacttctg tttgccttta aatcgccatt ggaagcggag cttgtcatag
    16321 aagggaaact cgataaacag gactcccaag cccacacaga gactggcaag gacgtctgat
    16381 gggtaatgaa ctcccagata gactcttgat accagcacac tgactaggta gaggccaagg
    16441 acgatttgta cgatttttct ccagactgga tctttaatcc gctgactaag aataacaatc
    16501 aaagtaccta ccatcaaggt tacagccaga gaatgcccac ttgggaagga aaatcccttc
    16561 tcctccacca gatgtaagat atctggccgt gggcgctggt agatattttt aaaggtcacg
    16621 attaaaagac ctgccaaagc cagatttccc aacatgaaga aactttctat cttccatcgc
    16681 ttacgataaa agacaaaagc tgtaatgaca acccaagtga taatcactgg gatatcaatc
    16741 agacgtgtga gggctcgaaa aagaatagtc aaataatctg gtaagtctcc tcgaatggca
    16801 gtctgaatcg attggtcaaa attgaccagc gtttcagggt aaaatttgac catgtagcca
    16861 agaataacga aaagtaaaag ggcaaaactg cccttcatta aaaatgtttg tttatctctc
    16921 ataatgtttt aaggttggtt tcaagagaac atacaacaac cagaatgaaa cggaaaagat
    16981 aacaccttca atcaagttaa aaggtaatac catggtcatt aggtagttgg aaagtcccaa
    17041 aatttttcca atatcaaagt tagcaaactt agcgtacaaa ggaacagcat aaacatagtt
    17101 gagaaccaac atggccaagg ttaaaccaat agttccagct agagagccta gtaggaaacg
    17161 aagggttgtc cgttcctttt tccaaatcaa agcaaatacg atgacaaaaa ctcccaaagc
    17221 tacgatattc atcggcaaac caatgtaagt attcactcct tggctgttaa gaagcaactt
    17281 caagagtgag cgaagcaaga gcactcctag agcagcaggc aaatccatga ccaccagacc
    17341 cacaaggact ggcaagatac taaattcgat cttgaggaaa gatgccgctg gtaaaagcgg
    17401 aaaatcaaag tacatcagca caaatgagat ggctgataga attgcaatgg tcgaaagtcg
    17461 acgtgtgttt gtcataacag gttcctccaa ttttctataa aatcagaaga agttggaaag
    17521 gattcctcta tctattctca ctttttatat cccaaaagtt ccctcttact ctattaaaga
    17581 aaaacaaagc aagtggttac aatccggcta taaatctatc aaaacagaca aagctattct
    17641 ttcgtcttct cccatccaga ctatactgtc ggttgtggaa tctcaccaca tcagcttgcg
    17701 ctcgcggact tctttaaaga gaaagaaata gagatttttc ttttagacta ggcgacaagc
    17761 tgtaggcata acttctggtt aggcaaagct tgtcaacaaa gtataaaagg aaaagatcat
    17821 ttcctagtta aagtcaccgc cggtcgggaa tcacaccctg ccctgaagac tcttttatca
    17881 taacaaaaaa cgcttgcaag cgcaagcatt ttgttcattt tcatttttta tttcaattta
    17941 tccacttcat aggtatggac gagctcaaga cctgcaaagt tctgctggcg aagagcctca
    18001 tagacaatca tgcagacggt attagacaca ttgagactgc ggacatgttc atcattcatg
    18061 ggaatacgga gagccttctc aggatgttct cgcataaaat cttcaggtaa gcccttgtct
    18121 tcacgtccaa agagaaaata atggtcctcg tcagtcgata aatccacctc agaatagatt
    18181 ttttccgcga atttcgaaat cagatagagt tttcccttca tctgagatat gaaatcttct
    18241 aaactctcgt aaaaataaat ctctagctta tcccagtaat ccaatccagc ccgcttcatc
    18301 ttgcggtcat caataggaaa gcccataggc ttgatgatgt ggaggggaga attggtcgca
    18361 gcgcaagtac gcgcgatatt gcccgtattt tgtggaattt gaggttcaaa taatacaatg
    18421 tgatttgtca tgacttgctt cctttcacca ttgcaaaaaa atagccacac tgcccggagt
    18481 caagctcagc aaacagcgtg gttaaggcat cgttaactta cctcacaaca ggtttgaagt
    18541 aaatcagcga aactactttc ttagtataac actttcagaa tcattgtcaa tagaaatgac
    18601 ttgatttttt caattttttc aagctatttc caagggttgt aaaatcgtcc ctgattctgc
    18661 aagataagta gtaaactaac tactaaaaac aaggttgcca agagcaaggt aatatagtct
    18721 ccttttttca aggcctgata actataccat gtgcgttttt tctctttccc aaagcggcga
    18781 agctccatgg cagtcgcaat ggtatcaatg cgttctagcg agctaaaaat caagggcgta
    18841 ataatgagca gattgccttt gattcgttgc ataagagaag ctttcttgga taattccatc
    18901 ccacgcgcct cctgagacat cttgatagta aagaattctt cctgcaaatc tggaatatag
    18961 cgcaaggtca ggctgacaga ataagcaatc ttatagggca caccaatttg atttaaactg
    19021 gaagcaaact gactaggatg ggttgtcatc aaaaagataa tagccagagg aatggtgcaa
    19081 agatacttaa tggccaaatt tagcagataa aagagctcct ggctggttag agtgtagaca
    19141 ccgattccct gccaaatcac acttctctct ccataaagtc caaccccata ctcgggagaa
    19201 aagagataga ccatcaaaac gtttaaaacg gcaaataccg tcgcaaaaac ggctacaaag
    19261 gaaacatctt taaagcgaat ttctgataaa tagaggagaa agactgaaaa gatggcaatc
    19321 agcaagagca ttctggtatc atagctaatc atggccgcca atgataccag aatgaaaaag
    19381 agaagtttcc cagctcctga caagcgatga atcacagtat ctctatgctg gtaaccgatt
    19441 aatttagctt gcatccctct ctcctttctt tgtaaaatgc cgttaaatcc agtggatcca
    19501 catctagttt cttagccaag ttaaagatgg aggtttcttt tagattggct tttactaaca
    19561 gctcaggatc gctcaacaga ctggctggaa cagtatcggc aatcaattct ccatccacca
    19621 tgacaaggac ccggtctgaa taatccagca tcaattgcat atcatgggta atcatgacaa
    19681 tggtatgccc tttttgatgt aactcttcga gaaattccat aatctcagta tagttcttct
    19741 gatcttgacc tgcagtcggt tcatctagga gaataatttc agctcctaag accaaaattg
    19801 aagcaatggt gacacgtttt ttctgaccaa atgacagggc agaaataggc caattacgga
    19861 attcataaag tccacagatt ttcaaggttt catagactct cgtttcaatt tccttctcat
    19921 ccacacctcg caaacggagc cctagagcca cctcatcaaa aatcatattg gttgaaatca
    19981 tttgattagg attttgtagc acatagccta ctcgttccgc ccgctctgca acagaatcgc
    20041 cttttatatc ctgtttttcc caaagatagc gtccttccgt ctgaataaag ctacttatag
    20101 ccttggctag agttgatttc cctgctccat tttttccgac aatagcaatc ttttcaccct
    20161 ttttaatatc taaatgtagg gattttaaaa tcggtctatc atcataagag aaagacacgt
    20221 cctctaattt aaagagtgac tgcaattctg gagtttcttt tgccagttca ttctgcagct
    20281 gaacctgacc ttttgagata gacatgttat ccagattcgc taactgttct tccttgacta
    20341 agtccacacc taattgacgg agagtcgtta gataaagggg ttctcgaatt ccattttgag
    20401 tcaataaatc agtcgcaagc aactggtcag ggctcccatt aaaaaggata cgaccatcgt
    20461 ttatcaagac aatccgatcc acagggcgat gcagaacatc ctccaaacgg tgctcgataa
    20521 tcagagtcgt cgtcccctct tccttatgaa tctggtcaat caattcgata atatcctgac
    20581 ctgacttggg atctagattg gcgagtggct catcaaacaa gagaatcgga ctttcatcaa
    20641 tcaagacacc agccagactg actcgctgct tttgtccacc tgacaaatcc tgaggacgct
    20701 gatccagtaa aggaagaagg tccagctttt cagcccattt ataaacacga cctttcatct
    20761 catctagggc tgtcacatca ttttccagag caaacgccaa atcttctgcc acagacaagc
    20821 caataaactg cccatctgta tcctgcaaaa ctgtgctaac cagatgagac ttatcataga
    20881 tgctcatatc aaaggctact tgacccttta tcaaaaattc tccatatgtc tgacccttgt
    20941 aaatattggg aataatccca ttcaaacact gacccaaggt agatttacct gacccagatg
    21001 gtccaacaat taagactttc tctcccttgt aaatggtcaa gtctatccct tgcaaggtcg
    21061 gttcttgttg tgtttcatac cggaaagaga aatccttcca ctcaattata gcttctttca
    21121 tcttactctc ttcattcgct tcttagactt ctattttatc ataaatcaag cccttcttgc
    21181 agtctcttct cttaaaatct tagcgccaaa aagattccta tcctagctta cttgcctaac
    21241 taatctataa acatcgaaaa agactagttg cccagccttc cccatcattt tatactcttc
    21301 gaaaatctct tcaaaccacg tcagcttcgc cttgccgtag gtatggttac tgacttcgtc
    21361 agtttcatct acaacctcaa aacactgttt tgagcaacct gcggctaact tcctagtttg
    21421 ctctttgatt ttcattgagt attagtcctt tttcaaactt cctgcacgag tttgggttcc
    21481 tgcataggca agtaagagaa gagttcctgc aatagctaca gatacaccat tggcaattcc
    21541 cgcaacaatc ccttgtgcaa atactttttc tgccgcttct tgataaatca caacatctcc
    21601 aagtggtgcc aagacacccc aaacaagggc atttgcaagt agttgaatga gattaaaaat
    21661 aagaatatct ttccagtcaa aaacactatt gatcacgcga acgtactttc taaaaagtcc
    21721 cacaactaaa ccaaagagtc cgctagcgat aatccaagtc caccatagac cataaccaac
    21781 aagagagtcc ttgattgcat gaccaatcaa cccgacaagc aaaccgataa tcggtccaaa
    21841 aataatagaa agtagcgctt gtaccgcata ctgaagctgg atgcttgtat ttggaacagg
    21901 ggttggaatg ttgatcatcc cgatgacgac aaagagggca gcgccaattc cgacagcaac
    21961 aacttgttta attgtaaatt tgatttccat actattctcc tattttatcc ttctattttc
    22021 tttatttcaa tggtccaaga tgaaccgaca cctacattat aggccttggc aaaggaacct
    22081 tggttgatag ccaaacctac acgatagaga gagttgatgt aaaggatggg ttgcccaatt
    22141 ctcacatctg caaatgattt gccatagaca acctgatttt gatagaccag catatcagca
    22201 tgatagatgg tcacttcaaa acgatcacca aattctggtt ccagcttgta aaattcttcc
    22261 cgtgtgatag aggtccaaag cgaaccgaaa cgcacatcca gaatatcaat ggctcccttc
    22321 accagatgat cttctatgat ggtcgctacg actggaagct ctacaatctg ttccacactg
    22381 agctctggcc ctacttcctc aaaagtaatg tgaccactgg ccagtttagc accagtatag
    22441 gcatagacat cacgaccgtg gaaggtataa gaatgctctg tgttttgacg cctattggcc
    22501 acctcagaaa tctcacgaat ggctacaatg ccaacgtgtt tcttgataaa ggaaagcgtc
    22561 ccattatctg gcgtgacaat gtattgattt tttgcagtct tggcaactac actcttacgt
    22621 ttcgaaccga cacctggatc gacaaccgat acaaacgtcg ttccctcagg ccagtaatcc
    22681 accgtctgaa agagacgata gctcccctca aaaatattat aaggcgtgat atcgtgcgtc
    22741 aagtgatgga tttttaaggt tggagactct tctaaagcca ctccaatcat agccgatacc
    22801 gcaccatcaa ccagaccaaa gtctgattgt aataccagta aattattatt cattttatct
    22861 ccttgtatat cctttatcat accatatttc agagaaagtc gctaatagct atttcaattg
    22921 gttagggtat agttttacct tatagcgaat tttcactaaa agctcttgtt attagctagt
    22981 aggtgcattt tttcttgaaa aagggtatga tagttacatc atgaaaaagt aggttttatt
    23041 atgaaaatta tccttgtcgg agggggaaaa gttggttttg ccctctgtcg ctccttggtt
    23101 gcagaaaagc atgatgtttt gctgattgag caagacgaag ctgttctcaa tcatattgtc
    23161 agtcgctttg atatcattgg tatccttggt aacggggccg attttgccat tcttgagcaa
    23221 gccagcgtcc aagattgtca tatctttatc gccctgactg agcacgatga agtcaacatg
    23281 attgcagcag ttctagccaa gaaaatggga gctaaagaaa ctatcgttcg ggtgcggaac
    23341 cctgaatatt ctaactctta tttcaaggaa aagaatattc tcggtttttc tcttatcgtt
    23401 aatcctgagc tcttggctgc ccgcgctatc gcgaatatca ttgacttccc caacgccctg
    23461 tctgtcgaac gctttgctgg tggacgcgtt agcctcatgg aatttgtcgt caagtccacc
    23521 agcggtcttt gccaaatgcc catttctgat tttcgtaaaa aatttggtaa tgtcattgtc
    23581 tgtgcgatag agagggatca tcaaattatc attccaagcg gtgacatgac tgtacaggat
    23641 aaagatagaa tctttgtcac tggtaaccgt gtcgatatga tactcttcca taattatttt
    23701 aaatcacgcg ccgtgaagag ccttctcatc gttggggcag gtagaattgc ctattatcta
    23761 cttggtattc tcaaagatag tcgtatcgat acaaaagtca ttgaaatcaa tcctgaaatc
    23821 gccagcttct ttagcgagaa attcccaaat ctctacatcg ttcaaggaga tggaaccgca
    23881 aaagatatcc tgctggaaga aagtgctcaa cactatgatg ccgttgcgac tctaacagga
    23941 gtcgatgagg aaaatctgat tacatctatg ttccttgaca gggtaggtgt acagaaaaat
    24001 attactaagg tcaatcgtac cagtctcctc gagattatca atgcgcctga tttttcaagt
    24061 atcatcacac ctaaaagtat tgccgtagac acgattatgc actttattcg tggtcgggtt
    24121 aatgcccagt attcagacct tcaggccatg caccatctag ccaatggcca aatcgaaacc
    24181 ctgcaattcc atatcaagga agctaataaa atgactgcca aacctctttc tcaactgaaa
    24241 ttaaaaaaag gggttcttat tgcagccatt attcgaaagg gcaagactat tttcccaact
    24301 ggggaggata tgttggaagt tggagacaag ctcctagtaa caaccttgtt gccaaacatc
    24361 accaagattt atgacttgat tgcgaggtaa gaaatgaata aaagtatgat tcgttacctc
    24421 ctttcaaagt tacttttgat tgaagctgtt ctcctcttgg ttcctgtggc tatcgctgtc
    24481 tattaccgtg aatcgagcca agtctttaca gccctctttt cgaccatagg gattctcgta
    24541 ttgttaggcg gttcagggag tttacagaag ccaaaaaatc aacggattta tgccaaggag
    24601 ggagtcttga tcgttgccct ctgttggatc ctttggtctt tctttggtgg tctccccttt
    24661 gtcttttctg ggcaaatccc tagcgttatt gatgcctttt ttgaaattag ttctgggttt
    24721 acaactactg gagcaagtat tttgaacgac atttcggttc tcagccgttc cctcctcttc
    24781 tggcgaagtt ttacccactt gattggaggg atgggagtgc ttgtttttgc acttgctatt
    24841 atggacaatg ccaaaaatag tcacctagaa gtaatgaagg ctgaggttcc tggtcctgtc
    24901 tttggtaagg ttgtatccaa attaaaaaac actgcccaga tcctctatct cctttacata
    24961 gccctctttt ccctctttgt catcatctat tatctagccg gtatgcctct ctacgatagt
    25021 tttgtcattg ctatggggac agcgggaact ggaggattta ccgtctataa cgatggaatt
    25081 gcccactatg gtagctcact gattacctat ctggttagta taggagtttt ggtttttggg
    25141 gtaaatttca atctctacta ctaccttatg ctccgtcggg ttaaggcctt ctttggagat
    25201 gaagagcttc gggcttactt ggtcatcgtg ctggtttcta caggcttgat taccctcaat
    25261 actctctacc tctacccagg attttccaaa agctttgaaa tggccttctt ccaggtttcc
    25321 aacatcatta caacaactgg ttttggatat ggagatatta ccaactggcc cctcttctcc
    25381 cagtttatcc tcctcttcct catgacaatc ggtggttctg ctggatcaac tgcaggtgga
    25441 ctcaagatta ttcgaggcct catcctttca aaaattgcca aaaatcaaat tttgtccatt
    25501 ctatctcccc accgtgtttt gactctccat gtgaataaaa cggtgattga taaggatacc
    25561 cagcataaaa ttctcaagta ctttgtcatc tatgctatga ttttgctatc ccttatcttt
    25621 attgtcagcc tagatagcaa tgattttctg atcgtgacca gcgctgtctt tagctgtttc
    25681 aataatatcg ggcctattct aggaaccact tctagtttct caatctttag tcctatctca
    25741 aaaattctcc tctcctttgc aatgattgca ggccgcttgg agatttaccc aatcctactt
    25801 ctctttatga agagaacttg gtctaagaga taaaatacaa aatccctctt tttacaattg
    25861 tgaaagaggg atttttatat taaaaagggg gaacgttttt aaatccaagc ctgtctgtaa
    25921 gcaaagttct tactcaaata aaagatggaa aaacttacta cacagcttgg taatgctcca
    25981 atacaaattg taaggcttta cttggattct ttgttaaatg aatatattcc attcctctgg
    26041 tagcaactat ctgaatacct aatctttctg catccacctt catctctcta taataactcc
    26101 gaacttgtgg agccagaata attaaatcat aaacacccat aatatcataa tgagctccgt
    26161 acgctcctga attcgcaatc actctaactt ctgctaattg agtgccctcg ttaattgcat
    26221 tggctaattg cgcacttgtt ccagaccctg cacaaagaac cagtactttc aactccttac
    26281 tttttattac atctatctca ccaggcatta tttcacttgt atcctcctct aaaataatat
    26341 catttgagct tgcaatatct acaacctgta gtaaatatct cgtatttaga caatatgaaa
    26401 acaagacgac ttccatatag gaaaccgcct tctctctatg ttgagtgatt tatattaaaa
    26461 taacttttct tctagctgca ttttattatt ataaaaacat tcatcataac ccccagaact
    26521 taaataacaa tttttattca agatacatac tcctagaata aactttatat gaaatcccca
    26581 tttttgtttt tacaattctc cttatactca atgaaaatca aagagcaaac taggaagcta
    26641 gctgcaggct gctcaaaaca ctgttttgag gttgtagata gaactgacga agtcagtaac
    26701 atctatacgg taaggcgacg ctgacgtggt ttgaagagat tttcgaaaag tattagttaa
    26761 atcttgttta atatctctac accatactat tttacatata ttatttagcg ccacatagta
    26821 ctgaactctc tccaaaaacg gttatgcctc tttgaatagg gcgttatcac aagaaaagca
    26881 tctccacgtt tcaacttcat atggctcaaa aacaatcaat tgatgctaaa acctgtacct
    26941 agatgtttcg gttcataaaa ccatgaaact gtaaaagtga atgaaattga tagcgatagt
    27001 caaatcaaga ggcatcataa ctctaaaaag tcacaatata taagttcatc ctcggaaaaa
    27061 tatcattcta attgttgaaa tgcctacatg aaaagaaacg tcaaatgctc atgaaacaac
    27121 gaatacaggt atcaaaacta tgacaaaaca agtccctaaa tttactaaag acactgctca
    27181 actttacacc tgtaagtggt tgttgtataa taaagttaca aagatgtacg accacactgt
    27241 tgtaaatcat agtgttcgcg aatatattac tgatagcatt tctacaaata caattgaaga
    27301 gactggatag actttaaacg aaatatgtca gtgttttggc attcctagcc ttcatatcat
    27361 ttaaagaatt ctatagacaa aattttttcc aatacagaca ctcgtaacaa ctgcttcatt
    27421 tttctaccaa catatttagg aacaggataa gatacaagag tattaatcca tagctcagtt
    27481 ctataccaat ctaagacaaa taagctaaaa aaacgattga taataagcaa atagattcca
    27541 aattttctct atctgctcat tttaataaac aatactagtg taactatcct tccagtcaga
    27601 agcttgtcaa atcacaccga aaattcttct aaaatttatc tcgttaggca atcaagcaaa
    27661 aactcgacga tagtacaaac attatcatac aggattgact tcctaaatta tatactttag
    27721 taaggttttc ggataagaaa aaaggttcat tttacatttc taaacattct tttctaagat
    27781 gaaaaacaga atttttcgat tgtgatttaa agcaacaaga agattttcag tatcatccta
    27841 tagatacgag ctaattaaga aaaactacat ttttgaatat aaactacaat aatataaact
    27901 aaattttata ggaggaagac aatggattgg tacgattata tgatacaggc atccaaacaa
    27961 tcacaattca acgcaagcca ttggtttcgc tatttgcgaa aagttatttt tgaagactat
    28021 tcttatttaa caaaccaaga tgtagaaaag ttgctagact ccaaagaact aacccgtttt
    28081 caaaaaatta gcttgaagta tgcctttcaa gagcatactc caactcataa atatgtgatt
    28141 tcattaaata aacctgctaa gttaaccaat gttcaaaaat tgatggagaa atacaaacat
    28201 ggataaaatg aaaccggtct tccaagccct aaataaggaa ttaattcagg aaaatctgac
    28261 tttaacaatt atctgtgtcg gtggttatgt cttagaatat catggtttac gtgccacaca
    28321 agatgttgat gctttttatg accaaaatca gaaaatcaat gaaattattg ctcgtgtagg
    28381 aaaacagttt aatcttaaca tccacgaaga gttatggctc aacaatcatg ttgcaaagca
    28441 aatatgaaca aacaacctcc cttaagttta tgcgaatcct tgtactcatt tgagaatctg
    28501 accgttttag tagtccctat tgagtatgtg ttaggaatga agatgatgag tatcagagag
    28561 caggatttga aagatattgg tgcaattatt aaatacaaaa actttcattc accattcgat
    28621 acctttaaat atttaaaaga catgggattt gatactattg acctctctgt tctattagaa
    28681 ggttttagtt atgcttatgg tatggattgg ttagaaaaat tttttaaaga aaatcaagat
    28741 aaacttcgcg aattttatta acagtatcaa acaagatgat tgtcaattag ccagtcgtct
    28801 tatttttatg atggaatgaa ataaaatcgg aacaaatcac ttagagaatt ccagtcaatt
    28861 tctagcactg tttgagacgt ttcagcgaac tatagtaaaa tgaaataaga ataggacaaa
    28921 tcgatcagga cagtcaaatc gatttctaag aatgttttag aagtaaaggt gtactattct
    28981 agtttcaatc tattatattt cagattcggt ccactattcc ttcccaaaaa cattgcatat
    29041 tgcgccgaag gaattgctca cactatccca agcacaaaac cctcagtcga ttgaccgagg
    29101 gttccttttc attattcaaa aacttgatta gctcaatgca tccaaggcat catccattga
    29161 aagaacttcg tggaagacac gttgtgtcaa ttcagttttt tgttctggag tgaggtattt
    29221 agtatttaca cagtatccag agatacgtac gataacgtct tcacctgaca taattttttc
    29281 gtaaacatcg ttcaagtcca taacgttcaa gttaacgtgt tgtccaccgt tttcgaagta
    29341 accatcaagg attgttacca agttatcaac ttgttcatca cgagtcttac caagagcgcg
    29401 aggtgatact tgtgtagtca atgagatacc gtcagctgca taactaaagt caaggctaga
    29461 aagtgagttc aagttttgca accaaccacc tttagctttg ttagatgggt tagcacctgg
    29521 tgagaagaat tcaagtttag acaagttcac agaaccatct tcgttgaggt atacaccttt
    29581 gtgaactggt gagttaccag tttgtttaga gtaagcaacg ttagatgtga ttgtcaaaag
    29641 tgatactgta gcttctgcgt ctttgtatag tttgtggcta cgtagacgag ttgtgtaagc
    29701 ttcgatcaac cattctgcca attcgtttga acgtgggtca tcttcacccc agcgtgggta
    29761 gtcaccgatt gtttcgtaat cgtagatgta gccatcttcg tcacggattg gtttaactgt
    29821 agcgtatttg atagctgaca atgtatcaac agtgttagca aatccacaga taccgaatcc
    29881 catgttggca cgttgtttag ttggcaagaa ggccatttga acagcttcgt agttgtacct
    29941 atcagtcatg tagtggatga tgttcaaggc atctacgtaa gtgtcagtca accagtcaag
    30001 agatttttca aagttcgctt taactgattc gaattcaaga acttcgtcac ggattggttc
    30061 gatatcaaat actttgtagt ctttgtgaac atcgtcgtaa ccaccattca aaccagtaag
    30121 aagggcttta agaacgttta cacgagcacc gaagtactga atgttgtggc gttgttcttc
    30181 attttctgga tcaagtggag acacacagca tgagatacag ctcatttcac catatccgtc
    30241 tttagccatt gttgttacac cttcgtattg gatagaagag tgtttgtggc tcatgtgcat
    30301 acagtagcga cggaagttgt atggcaattt gtcagtccaa agaactgtca agtttggttc
    30361 tggtgagtta ccgatgttgt caagagtgtt caagaaacgg tagtccatct tagtaacacg
    30421 gtgacgaccg tcgttaccca taccagccat agaagttgtg ataaaggttg ggtcacctga
    30481 gtacaattgg tcataagctt ttgtacgagc aaatttaact gtacgaagtt tcataacgaa
    30541 atcatcaacg aattcttgga tttctgattc agtaaatgta ccacgagcaa ggtcacgttc
    30601 tgcaaagatg tccaatacga ttggtacacg acctagagat gtagcagcac cgttaatcac
    30661 acggcagaca gccatgaaag caatgttaac ccattggatt gcttctttca cgttcatcgc
    30721 tggtttgcga acatcaaccc cgtaaaggtc acccaggcga acaacttgtt gcaatgcttg
    30781 gtattgaagg tttacttctt cacgaagacg gattgtttct tcatcgattt ctttgattgc
    30841 attccagtca tttacttttt cttgcatcaa gtagtctgca ccgtaaagag caagacgtgc
    30901 gtaaacaccg atgatacgtc cgcgtgagta tgcatctgga agaccagtta cagtgtgtgc
    30961 gtgacgagcg cgacgaatat ttgaagtgta ggcacggaaa ataccgtcgt taactgttgt
    31021 tacatattta gtgaagattt cgtgaacagc tgggtctggt tcgtatccat tttctttcaa
    31081 agtagtttca gccatacgga taccaccttt tggcatgaag ttcaatttga agagttcatc
    31141 gttttggata ccgaagataa cttcattttc tttgtcgata aatccagcag ggatatcagc
    31201 gatagatgtt ggacgagtgt ccattgggaa acgagtttct tcgtagtgtg ctttagtttc
    31261 ttctacaatt ttcttgatgt gaagtgaacg ctctgttggt cctgcaagga agctttcgtc
    31321 tccatcataa ggtgtgtagt tagcttgtac aaagcgtgat acacttgctt tttctttcca
    31381 atctacgcct ttgaagcctt cccaagcttt gtcaaaaata tcttgtgctt caacaactgt
    31441 cttaacaacc atattaatgt cctctttttt ctagtaacag tcatctgtta cattcatgag
    31501 acaagtatac catatagtaa ccgatttcaa caagtgaaaa atccctattt ttacactttc
    31561 ttttctaaaa cagtctatat tttatttcaa actgtattat atttttgaaa aaataaagtc
    31621 cttttttctt tttttcagaa aaaagggtat aataaaagaa aataagcagt aacactcaat
    31681 ggaaatcgaa aagcaaacta ggaagctagc cgcaggctgc tcaaagcact gctttgaggt
    31741 tgtaaataga actgacgaag tcagctcaaa atactgtttt gaggttgcag atggaagctg
    31801 acgtggtttg aagagatttt cgaaaagtat aaaaaggtgc taggcatgtt gatttttcct
    31861 ttgctaaatg atttgtcaag aaaaatcatc catattgaca tggatgcctt ttttgctgca
    31921 gtggaaatca gggataatcc taaactcaga ggaaaacctg tcattattgg aagcgaccct
    31981 cggcaaacag gtggacgggg agtcgtttct acctgtagtt atgaggcaag agcttttggt
    32041 gtccattctg ccatgagttc caaggaagct tatgaacgtt gtccccaggc tgtctttatc
    32101 tcagggaatt atgagaaata caagtctgtg ggactccaga ttcgagctat ttttaagcgc
    32161 tatacagatt tgattgaacc catgagcatt gacgaagcct atttggatgt gacagaaaat
    32221 aaactcggta tcaagtcagc ggtcaaaatt gctcgcctca ttcaaaaaga tatctggcaa
    32281 gaactccatc taactgcttc cgcaggcgtt tcttacaaca aattcttagc taaaatggcg
    32341 agtgattatc aaaaaccaca tggtttgaca gtgattctac ctgaacaggc tgaggatttt
    32401 ctcaaacaaa tggatatttc caaatttcat ggagtaggaa aaaagacagt agaacgtctt
    32461 catcaaatgg gcgtttttac tggtgctgat ttacttgaag ttcctgaggt aaccctaata
    32521 gaccgttttg gtagactagg ctatgatctg tatcgaaagg ctcgtggcat tcacaactct
    32581 ccagtcaaat ccaatcgcat ccgtaaatca atcggcaagg agaaaaccta cgggaagatt
    32641 ctccttgctg aggaagatat caaaaaagag ctgactcttc tatcagaaaa agtcgctctc
    32701 aatctacatc aacaagaaaa agctggaaaa attgtcattt tgaaaatccg ctacgaggac
    32761 ttttcaactc ttaccaaacg aaaaagtctt gctcaaaaaa cacaagatgc tagtcagata
    32821 agccaaatag ccctgcaact ctatgaagaa ttaagcaaga aagaaagagg tgtccgccta
    32881 ttggggatta ccatgactgg attttaaagc tcaatgaaaa tcaaagagca aactaggagg
    32941 ctagccgcag gctgctcaaa acactgtttt gaggttgcag ataaagctga cgcggtttga
    33001 agagattttc gaagagtata aaaccttgaa gagttgcccc ttcaaggttt ttcttataca
    33061 aataaacgga caaaactgta aagtagcaaa acactaccaa gcacgatacg gtatttacca
    33121 aaaagggtga agtcgtgttt tttcacatag ctggtcaaga agcgaatagc caccatgctg
    33181 accgcaaaag ctactcccat cgcgaccaag agcaaaaaca attgcccaaa gctcaagagt
    33241 tctccggctt tcacaaattt gaaaatcttt aaggcactag ctccaaacat aacaggaatc
    33301 ccaagataga aggtaaattc tgtcacaact gaacgactgg ttccatttaa caaaccaccg
    33361 acaatcgttg cacctgaacg gctagtccct ggtaaaagag caagaacttg gaagagtccg
    33421 atatagaaag cggtcgtata aggaagcttg tccaactctg ttacacttgg ctcgatagca
    33481 cgcgctttat tgcgcttttc caaatagatg aaggcaaccc cgtagataat caacatgaga
    33541 gcaactgaaa ccatgttatg gaagtgggta tcaaaccaat catcaaattt aaagacacca
    33601 agtaaaggta aagtagcaat caagaccttc aaccatagtc tccaagtctt acgaacttcc
    33661 tgtttgtcct tggtcggttt aaaaggattg agcttgttaa aataaatcac cataactgct
    33721 aaaatagcac caagctgaat cacgacatta aacatggaca taaaggcttc attttgattt
    33781 tggtattgga tgaattcctc tgctaaaatc aagtgacctg tactggaaat cggcaaccat
    33841 tccgtaattc cttcaacaat tccgaagaag atagatttta aaatttcaat aagatacata
    33901 gattactccc tatattatat ttagcgcagt tagctactgc caaagcccaa gtggtatact
    33961 tggaataagc cactatggat tagttcattt cctccaatcg cttgacgatt tccgaaaggc
    34021 tctaaccaca gcttttgttt caattggtaa tgagtttgat aacgcataga cgtttgatct
    34081 cgtctaaggt aacaaggcaa aaaattcgtg gataaccaag tagacttgaa atactatatc
    34141 tggaggtact cccatgcttt atgtaggcat tgatatcgct aaaaataaac acgatgttac
    34201 agccttgaat gttccaggaa aaactgttct taaaccactc actttttcaa ataataaagc
    34261 tggttttgaa ctcttagatc tgtctattcg acaactcaac caagactgtc tcatcgctct
    34321 tgaagatac
//
LOCUS       DAVDWM010000018        33077 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVDWM010000018 DAVDWM010000000
VERSION     DAVDWM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.018"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(61..942)
                     /locus_tag="VUW49_001061"
     CDS             complement(61..942)
                     /locus_tag="VUW49_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET7980992.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(1083..2411)
                     /gene="vncS"
                     /locus_tag="VUW49_001062"
     CDS             complement(1083..2411)
                     /gene="vncS"
                     /locus_tag="VUW49_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET7980993.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(2408..3064)
                     /gene="vncR"
                     /locus_tag="VUW49_001063"
     CDS             complement(2408..3064)
                     /gene="vncR"
                     /locus_tag="VUW49_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET7980994.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(3113..3196)
                     /gene="pep27"
                     /locus_tag="VUW49_001064"
     CDS             complement(3113..3196)
                     /gene="pep27"
                     /locus_tag="VUW49_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET7980995.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(3376..4755)
                     /gene="vex3"
                     /locus_tag="VUW49_001065"
     CDS             complement(3376..4755)
                     /gene="vex3"
                     /locus_tag="VUW49_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET7980996.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLVAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(4807..5454)
                     /gene="vex2"
                     /locus_tag="VUW49_001066"
     CDS             complement(4807..5454)
                     /gene="vex2"
                     /locus_tag="VUW49_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET7980997.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(5467..6744)
                     /locus_tag="VUW49_001067"
     CDS             complement(5467..6744)
                     /locus_tag="VUW49_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7980998.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(7102..7272)
                     /locus_tag="VUW49_001068"
                     /pseudo
     CDS             complement(7102..7272)
                     /locus_tag="VUW49_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(7415..8014)
                     /locus_tag="VUW49_001069"
     CDS             complement(7415..8014)
                     /locus_tag="VUW49_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7980999.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNTK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(8151..9035)
                     /locus_tag="VUW49_001070"
     CDS             complement(8151..9035)
                     /locus_tag="VUW49_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET7981000.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(9039..9425)
                     /locus_tag="VUW49_001071"
     CDS             complement(9039..9425)
                     /locus_tag="VUW49_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET7981001.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(9418..10761)
                     /locus_tag="VUW49_001072"
     CDS             complement(9418..10761)
                     /locus_tag="VUW49_001072"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7981002.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(10848..11732)
                     /locus_tag="VUW49_001073"
     CDS             complement(10848..11732)
                     /locus_tag="VUW49_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981003.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYQLAKLSNLEVLLADSSY
                     QEQISQLKFQAFSGEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(11744..12361)
                     /gene="cysE"
                     /locus_tag="VUW49_001074"
     CDS             complement(11744..12361)
                     /gene="cysE"
                     /locus_tag="VUW49_001074"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET7981004.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(12377..14590)
                     /gene="pnp"
                     /locus_tag="VUW49_001075"
     CDS             complement(12377..14590)
                     /gene="pnp"
                     /locus_tag="VUW49_001075"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7981005.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(15275..16141)
                     /gene="metF"
                     /locus_tag="VUW49_001076"
     CDS             complement(15275..16141)
                     /gene="metF"
                     /locus_tag="VUW49_001076"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET7981006.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(16205..18454)
                     /gene="metE"
                     /locus_tag="VUW49_001077"
     CDS             complement(16205..18454)
                     /gene="metE"
                     /locus_tag="VUW49_001077"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET7981007.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGMKADDFVDSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRTKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(18714..18980)
                     /locus_tag="VUW49_001078"
     CDS             complement(18714..18980)
                     /locus_tag="VUW49_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981008.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARNKQGISQKRLEEL
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            complement(19065..19553)
                     /locus_tag="VUW49_001079"
                     /pseudo
     CDS             complement(19065..19553)
                     /locus_tag="VUW49_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            19709..20710
                     /locus_tag="VUW49_001080"
     CDS             19709..20710
                     /locus_tag="VUW49_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7981009.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRVKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPGTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(20778..23183)
                     /locus_tag="VUW49_001081"
     CDS             complement(20778..23183)
                     /locus_tag="VUW49_001081"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7981010.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNIGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKTIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFARLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPAAPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(23260..23769)
                     /locus_tag="VUW49_001082"
     CDS             complement(23260..23769)
                     /locus_tag="VUW49_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981011.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(23769..24815)
                     /gene="pheS"
                     /locus_tag="VUW49_001083"
     CDS             complement(23769..24815)
                     /gene="pheS"
                     /locus_tag="VUW49_001083"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7981012.1"
                     /translation="MSTIEEQLKALREKTLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(25298..26713)
                     /locus_tag="VUW49_001084"
     CDS             complement(25298..26713)
                     /locus_tag="VUW49_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET7981013.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTILKRYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(26726..28564)
                     /locus_tag="VUW49_001085"
     CDS             complement(26726..28564)
                     /locus_tag="VUW49_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET7981014.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(28582..29421)
                     /locus_tag="VUW49_001086"
     CDS             complement(28582..29421)
                     /locus_tag="VUW49_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET7981015.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPGEYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(29721..32383)
                     /locus_tag="VUW49_001087"
                     /pseudo
     CDS             complement(29721..32383)
                     /locus_tag="VUW49_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            32931..>33077
                     /locus_tag="VUW49_001088"
     CDS             32931..>33077
                     /locus_tag="VUW49_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981016.1"
                     /translation="MGQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     ECGSQ"
BASE COUNT         9659 a         7276 c         5965 g        10177 t
ORIGIN      
        1 aaatttgtcc tttctcgagc ttagcttttt ttcaacccac tacagttgac aaagagccga
       61 ttatgcttta ccttctgaac cgaatacgtc gatacgttct tcaactgatg cttggatagc
      121 ttttacaccg tcagccaaga atttacgtgg gtcgaagagt tttttcttgt cgtattctgc
      181 ttcgtttgct tcgtagtcac gagcaaattt acgagttgcg ttagcgaatg cgatttggca
      241 ttctgtgtta acgttaactt tggcaacacc aagtttgata gctgcttgga tttgctcatc
      301 aggaatacct gatccaccgt gcaatacgat tgggaatcct ggaagagctt ctgtcaattt
      361 ttgcaagtgg tcaaggtcaa gaccttccca gtttactggg taagggccgt ggatgttacc
      421 gataccagct gccaagaagt cgataccagt ttcaaccatt gctttagcgt cttcgattgg
      481 agccaattca cctttaccga tgattccgtc ttcttcacca ccgatagtac caacttcagc
      541 ttctactgag atacctttag cgtgtgcttt ttcaacaact tctttagcca atttaaggtt
      601 ttcttcaact ggaaggtgtg aaccgtcaaa catgattgaa gtataaccaa cttcgataca
      661 ctcaagtgca tcttcgtagt gaccgtggtc aaggtggata gctactggta cagtgatacc
      721 cattgattca acaaggttag cgatcaagtt gcgagcaact ttgtaaccac ccatgtattt
      781 agcagcaccc attgaagttt ggatcaaaac tggagctttt ttagcttctg ctgcgcgcaa
      841 gatagcttga gtccactcaa ggttgtttgt gttaaatcca ccaactgcat aaccgttgtc
      901 acgggctgct tggacaaatt tttctgctga aacgattgcc attttatcag gcctcctgta
      961 tatttttatg ggtcatccca tttacattgt tcattttatc actttttgcc aaaaaaatct
     1021 agtttttccc gcagtttcga ttgattttct tctaactcca tctatgtaaa ccctttctct
     1081 ccctagtctt ggacgacttt tggaaaatct ataaagaagg ttaaacgatt ctcctccatc
     1141 tcgaaacgat aagctaattt ttcatgttct aatagactct taaccacaaa gagccccata
     1201 ccagacccct tgaccttgcg actggcattg tcagaaaaag actgggctag tttttcttgt
     1261 tcctctgagc tacagctatt ttcgataaaa agttctcctt ctctttctcc aattcgaact
     1321 aagccacctg gaacagagtg cttaatggca ttgctgatga gattagaaag aatcaacttc
     1381 ataactgatg ggtttagata agcctgctga tgggtcaaac tattgtctat ctggagctct
     1441 ctttccttgg ctagcaaggc ataatctttg accagatttt gcgtcatctg gaggaggtca
     1501 attgtttccc tatcatctcg caattcctgc acagaagaga gggaaagtat ctgcagaaca
     1561 tggtgattga gttcatccac aatccccaag gcaactccca gatactggtc tctatcctta
     1621 taacgaccga tattctctct catattttcg attaggattt tcaaactagc cagcggtgtt
     1681 ttcaattcat gagaagctcc tcgtaggaat tcgaccttca tcttctccag ctggagaatg
     1741 gcttcattct tttcatgcaa gtccgcaata acagtcaaga gatgctggta gaggctattg
     1801 atttgttcct tgagattacc tatctcatcc ttagaatcca cgcgcaatcg cacttgggaa
     1861 tccaggtcca tcatccgacg ggtcacccgc ttgatttcca aaatcggtgc aacaatagtc
     1921 cgagcgtaga tgtaggccac caagagggaa atcagaaagg aggccagcaa ggtataggga
     1981 agaaactgga gactgatttg ctccgcttcc ttttgtaaat ccatggaagc tagaaactgg
     2041 agaatcatag tagcaccgtc ttgcgttttc acctcgcgct cctcaataaa gagagaggtt
     2101 gtctggcggt ctgtgtccag aggaagactg tccttgactt ctaacttgtc ctcggtcatc
     2161 tctcctttga cggtcccctt gatatcacta gtctgggaat acaagtctaa cacttgctcg
     2221 atactctgcc tatctttccc ttctagggac tgggcaatgg ctgttgcctt ttgaccaatg
     2281 gtttcctgac gatgactcag ataagtcgaa ggaaaaagaa aataaatagc taaatgaagg
     2341 cagataacca gaacactaaa tatcgagaag gtatagataa atatctttgc aaataaacct
     2401 gttcgtttca ttttcgctcc aatttataac caacattgcg cacagtgagg atacaatcca
     2461 agtctagctt tttccgcaat tccttgatat aaacatcaat aacacggtca aagggaacct
     2521 catctgtcgc tttccagacg gcatcgataa tctgagatcg agtcaaggcc cggccttcat
     2581 ttttcactag atagtccaga atttccaact ctttggcatt gataggcact tcttgacctg
     2641 cgaggcttgc actgtagctt tcaaagtcca ccttggtatc cttgtaagaa aagattcgtc
     2701 ctgtatcgta gtagcgcttg aaaatcgcgt ccaccctcac ttttaaaagg gagagggaga
     2761 aaggtttttc cagatagcca tctgccaaag aggcaaaggc actcatcttg tattcctcat
     2821 cctgaaaagc tgtcaacatc aagacaggaa cctgactggt tttacgaatc tcagctaaga
     2881 cttctaagcc gttgagcttg ggcatctgga tatccagtaa aaccagggcc acctcatagc
     2941 tagaaaattg ctccagagct tcctgaccgt ccgctgcctc aatagtttca tagccacaat
     3001 ccgtcaaata atcactgacc ccctcacgga tcatctcttc atcttctaca attaaaattt
     3061 tcatacttta actgctctct attttttatt tttcttagaa taaataccta ctctattttc
     3121 tattatagtc tcttgctggc cttttgtctg caagcaactg accactagat aaaacgttgt
     3181 gaaattcctt tctcataaat tccataactt tagtatatta tatttaagca ctaaagtaca
     3241 aagaaagcaa ctgaaagcaa tgattttcac cactgctttc agatttattt tgaattgtta
     3301 aatagccatt cctatccact attcttgaat agaaacacaa gatgcaatct ttattccaga
     3361 ctcatttttt caaatttatt caccatccag caagagttct tttggttgtt ttctaaggag
     3421 attgcttgaa gcaagcgcca taacgagaac cactagaacc aaggcaagga caaaaatgat
     3481 gataaagtct gatgtctgaa tggaaatgtc taggctcgac aaggtcttgc taaagccatc
     3541 tacttctgca ccaccaccaa ggttagaggc ttgagccgcc ttactagcct gtttggcaac
     3601 acctgaagtc acattggcaa ggacagtgtt tccaattgca cgggcagtgt aattagctag
     3661 gaagtaagca gaaactagag cagggatagc aatcaagata gattcggtga tgaattgacc
     3721 caagatactt gcctgcttga ggccgataga gaggagaatt cccacttcct tgcgacgggc
     3781 gttgatccaa aggctgagca agagggcaag gaggagaact gagaagctca agctacccca
     3841 gaagaggagg ttggccatct tgtacatacc agagatagat tgctcaagag ctgggtagtt
     3901 agaggagctc ttgacgagtg tgtagctctt ccagttgata ccactgatgc cattcaactc
     3961 tttcataaca tcatccaagt tcttgtctgc tgttacaaag aaggttgcgt ccccataaat
     4021 ggctgtgtct tctgtgtatc cataaagttt tgcagcagtg tgaatgtctg taatagctgt
     4081 gttttcgtaa agttcttgtg agtaggttac tgctgactta ttatgaccat caaagagtcc
     4141 cttgattgtc acttcaactg tttccttggc tcctttttca ttatctgcat cgtagatatt
     4201 agagtccagt ttaaccttgt cccctacttt ccagccgtgt ttggctacca agtccttgtg
     4261 caagaggatt ttatccttgt cgtcgttggt taagtgctct ccttcgacta gtttataaga
     4321 accagagaca aacttgtctt ctttagagga gtcattgaca cctgtaatca tcaagctact
     4381 tccaaaacgt ttggcacgat cagcagtgag attcttcttg gtttctggcg tttcaatcag
     4441 gtcatatcca gtcaaatctc cgatagcgtt gatacgtttg acataagact caatggcctt
     4501 gttttcggtg atttttttga tgtcttcacc cttgatattc ccagcaccgc gaggcgttcc
     4561 ttggttgacg cgacgattga tttgcatgga gaagctattg gtgatatttt taaaggtctc
     4621 ctgagaagcc ttggcagtag ctcccttgat tgacaagccg accaaactca agctcgccat
     4681 gaggagaata atcaggaaga tgacaatcga tttgaaaaac ttccttgtaa cataggcaaa
     4741 tgcgttgtgt aacatagatt ccctttctag attttgtttt aatcattcta ttaaagtaag
     4801 ctcaaattat ttactagtat tgcgcgtttc agtcagtttc ttatccttta attcaagtgt
     4861 aatatctgac gcttgtgcca cttctttact gtgagttacg acaatcacac atttacctgt
     4921 tttctgggca agtgatttga gtagttcgac aatatctcca gcagttttag gatccagatt
     4981 tcctgttggc tcatcagcta gaataactgg agcttctgag accaaactgc gagcaatggc
     5041 aacacgttgc tgttgaccac ctgataactg gagaacattc cgcttgatct ggctttcatc
     5101 caaaccaagc tcaagaagtg tattcttgct tgcctttttg ttgaccaatc ggatattttc
     5161 cagcggagaa agataatcta tcaagttata attttgaaag accagggaaa tatggtgcat
     5221 gcgatggtaa gaatagccct tcttacgaat atcctctcct tgaaaaagga tagaaccttc
     5281 aacaggacta tctagaccag caagtaggga caagagtgtg gattttcctg ctcctgactc
     5341 cccaataata ctgtaaaatt ttccgggttc aaaattataa ttgatctgat ataggactgc
     5401 ttcagcagta ttcttataac ggtaggtaac atcttgtaat tgtaataaag tcatgatttc
     5461 tccttcttaa ctaatagatg ataaaatttc tttcggtgat tttctaaata agaataggaa
     5521 acaaagggct acagataagc aactaagcag aactagaaaa acataggatt ctgcaaaaga
     5581 taagatgcta gttgataaac tgcttgcttt ggctagtgta tcttgtaagc ttgcctgatc
     5641 tccacttgct agtagagttt ggagtaggta agttgtgatt ccgtttcctg caacaaatgc
     5701 tggaagcaaa gctccaagag ataccaaaac tacctctaaa cagaattgta ggaagatcga
     5761 gctcttgcct tttccaagtg caagtaaaat ccccacttca tagacccgtt ctctcaacca
     5821 gagagacaaa accagaatta aggctccagc tcctgctatc aacatcccat aaaggaagat
     5881 ggtcaggaag gtttggaaag ttgcaactga gtctttgatt tgttcaaaag ccttgttttc
     5941 cttttcgact tggtagcctt gattttccaa ggccaagttt tctacctgct tcatgagtcc
     6001 gtccatttcc ttaggatttt ctacatagaa gcgtgctgca ctgacttgag cttcactatt
     6061 gcccaaaagg gtttggctac tttcatagtc tgtaaagact tgattttcac tgaagtcaga
     6121 agacaagcct gtgaatttct cttgtttttt accagaaaag atgccaataa tctcaaactc
     6181 tactgtttgt ccttttccag attcagactg accagcatcc aagccaatct tgtcatgaag
     6241 tgaaagaccg ttcttcttag ccaattcttc gtggataagg attttcttgg aatccccttt
     6301 ttgaaggtgt cgcccttctt ttagattgaa agccgaactg gtaaaggtta catccttgga
     6361 tgaatcctca agagccgtta agctaaccaa gttattgtct gcagctgata aatcatcacg
     6421 ctccacgctc tgctcgccag tcactgcttc cttgtctttt agttttgcga ccgtctcaag
     6481 ttcaggagag acattttcca gccccttaat cttgcttaca gatgctaggt ctgacaactt
     6541 gaatgtctga ccattctcta tcttcttaat agaaaaagat gtattgagtg atttataaag
     6601 attgctttct actgttttgt tggacttcat cagagtcaaa caggctgaaa ttccggccaa
     6661 taagaccaat aaaatcagaa ataaaataaa acttctcagt cgctttctgc tgacataagc
     6721 ccaagatctt tggattggat tcatttgtca cctccatatt tgtaagacta ttataaaacc
     6781 caaatatgaa atgtttatga aatacgaaaa aaaaaatatc gagtagggga taatctctag
     6841 cccctctcac accaccgtac gtgccgttcg gcatacggcg gttcaactaa cttttaacgc
     6901 atgtcgttca aggtaataat ccaaacacga aaccagtcca cgttttccaa ggactggttt
     6961 tgatatagca cgtttaagta ccgacttctg agctactata gtagattgaa actagaatag
     7021 tacatctctt cttctaaaac attgttagaa atcgatttga cttccctgat cgattcgtcc
     7081 tattcttatt tcattttaat ataattgata gtggtcgccc cagccagata ccttatctgc
     7141 tatccattta ggaaccccta acttaagcaa tccccataat cgtctcgatt tcttcttcca
     7201 ttgcttccag ataatcactc gtaggcgagt acgcaagcgc tcatctatgc tagtgactat
     7261 acttttcata tttataattc attcctttcg tttcactcaa ggcacaacac agaatgaaaa
     7321 agtgttgtga tctttatttt gttttataat aatagtgaga aagcctatca ctactacaaa
     7381 tcacgaggag gtgaataagt gagtggtaca gccactacct cgcatatttt gtcacatcat
     7441 ttaacggtac ataataagtt gtaccatctg aataagttgc tacaatatca tttgcatgct
     7501 ctccttcacc tttagcaaag gttggagctc ctgctggatg atttttattt gcctctttca
     7561 atttttcaat aatggcattt ttttctgtat cttttatatt atcaggattt ttcactaaga
     7621 ttttgtctgg atatgtcggt ttagcagaaa caatttttac tgttatttct tttttattcg
     7681 aagcacttgt ccagtttcca gcattatctt tagtatttaa ttttacagta attcctgaac
     7741 taggaacttc agtagcaggt tgattatcaa cattattcaa ctttaatttc aaaagagctg
     7801 ttgcatcaga cgttttatca atcgttatat ataatgataa attgttatta taaccagttt
     7861 cttcatattt agctgtttgt gagctacttg aaacagaact gaaattatgc ccactacctc
     7921 cctgattatc ttcaatgctt acgtctaaat gaacttcccc actattattt ggcttagcaa
     7981 caactgttat agtaaaataa cataaaattt gcataaatag attagggaaa tcaaagcaac
     8041 ttctaggaat gttttagcag tcacagtgta ctttcccagc atcaatccac tataactctg
     8101 cacataaaaa tggagaagat ggccatcctc ttctccaaat attaacttct ttacaaacca
     8161 actatagttg acaaagaacc taaaatcaat tgataacaca aggtcaggtc ggtcaactct
     8221 ttcaactgaa gccctgtcaa ctcttcccat ttatcaatct tgtattggag agaattgcgg
     8281 tgcagataga gttgctgggc tgttttagtg agaacagcac tattttccca aagagagaga
     8341 atgatttcct gaatctgatc ttgatccaaa atcatctggt gtagacattc tttaattggc
     8401 ttcaagtcca cgagtctttc tcccatactc caaagataga gctgagaaaa agtatgaaca
     8461 ccttggtgac cctgacgcca ccatgtcttg aacaaatccc gctcagcttt gattaagtct
     8521 gatagggctt gatgtcctgt ctgagaccaa acctgaccca acatgataga aagacgaagt
     8581 ccaaagtcat actcaaccgc ttcaatcgta tcacttaaaa tatctcttac agaagtgtat
     8641 ttgtcttgtt gaagcacgaa aacataatcc tgagatccga cctgtagcac tgtctgacaa
     8701 ttcggaaaaa gagtccgcat catatctagc caagaagcca gattttcctg ctgaaaataa
     8761 gaaagatggc aataaaccaa ctgaatcttt ttaaaaactt gcggtgcctg tcccttgcct
     8821 tcaaccagat aggaatacca agggtttagc gaacgagcct gctcctgctg ggtcaaaagg
     8881 gcaaccaact gcttttcacg ctcgctgagc ccagcttcct ccagcaaaat ccactgctga
     8941 gaagctaaag ggagcgtgag atagccctct ttctctactg gttggtctga aatccgagcc
     9001 tcaggaaacc agtcttgtag ttctttttcc ctcatgttct agccctccac tttttggatg
     9061 caccatgaaa ccaaactctc aagacgttcc agattctcag tcatatggag atagcccata
     9121 accgcttcaa atcccgtgga catacgataa gtcacgacat ctgcattttt agcctttgtg
     9181 tggctattgg tattgcggcc acgtttgtag atttcttctt ctttttccgt taggacctgc
     9241 tcctccaaca tgagagcaat caggcgagcc tgagccttgg ctgacacata cttggttgct
     9301 tcttgatgga gtttattggg tttggtcata cctttgagga tgaggtgacg gcgaatatac
     9361 atagaataca ccgcatcccc ctcaaaggct agcgcaatcc cgttaatgag attgacatca
     9421 atcacgtgtc cacctcactc catccttggt atcaaggagc ttaattcctt gagtaaccaa
     9481 ttggtcacgg atttggtctg ctgtctcaaa gtctcgattg gcacgcgcct cttggcgttt
     9541 ttgaatcaag tcttcaatct ctgcatccaa aacttcctca acaaagacaa ttccaaaaat
     9601 ttctaacata tctgcaagag cttgcttgac acttgcatca tagttccctg agttgatcca
     9661 tttggccatt tcaaagacaa ctgtgatacc gttggcagca ttaaaatctt catccatagc
     9721 tgctacaaac ttatctttaa agttttgtaa ctcttgggca tccacgtttc ctgtaaatgg
     9781 ttgttcgtaa gtattcttca gatacttgag attggtctcg gcatcgcgaa ctgccttttc
     9841 cgtgaagttg ataggcttac ggtagtgctg ggtcgcaaag aagaaacgaa gtacttgccc
     9901 atcaagagtt ttaagggcat cgtgtaccgt aatgaagtta cccaaggact tagacatttt
     9961 gacattgtcg atattgacaa agccattgtg catccagtag ttagcaaaag ccttgcctgt
    10021 tttagcttca gattgggcaa tttcattggt gtggtgtgga aactctaggt cagctccacc
    10081 accgtggata tcaatggtat cacctaaaat ctctgtcgac atgactgaac actcaatatg
    10141 ccaacccgga cgtccaggtc cccaaggact atcccaagaa atctcacctg gtttggaaga
    10201 tttccataga gcaaaatcta caggattttc cttacgagcc gtttcttcat cggtacgacc
    10261 tgaagcacct agctccaaat cttccaaggt tttattagcc aatttagcat agttgtggga
    10321 tttttctaca cggaaataga catccccttg actctcatag gcaaagcctt tctcgatcaa
    10381 gtcttccaca aaacggatga tgtctgccat aaactccact acacgcggat ggcgagtcgc
    10441 aggtttcacg cctaaggccg tcacatcctc acgaaaggca gcgatgtact tatccgcaac
    10501 ctcctgaggt gtgatgcctt cttccctggc acggttgata atcttatcat ccacatctgt
    10561 aaaattggaa atataggcaa ccttataccc acggtactca aaatagcgac gaatcgtatc
    10621 aaaagctacc gtcgaacggg cgtttcccac gtgaatatag ttgtacacgg ttggcccaca
    10681 aacatacatc ttgatcttgc cgtcctcaat cgggacaaat tctcgcaaat cacgagacat
    10741 ggtgtcatag attttaatca taaatcataa tcaagaaagc taaaatccaa gaactgttag
    10801 tttcatcact aaactaagag ctcaagtaaa tttcagtccg aatgtctcta cacttcggaa
    10861 tcccttgctc ctttctcatt cagataaacc acctgtgtct gtttgacaaa gccaattttt
    10921 tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa ttccttatca
    10981 ttttgctcaa ttagttggtt gacaagggat tttgctaagt agcttccata gccttttcca
    11041 cgttcaggtt ctgatattgc taaaccgtag aagtaattcg tatcgctcga taaatcaacc
    11101 gtgcaagtcc caataacctg accgtctttt aacaaaatat ataagcgact ttctggatcc
    11161 ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc ccctgaaaat
    11221 gcctgaaatt ttaactggct aatttgctcc tgatacgaac tatctgctaa caaaacttca
    11281 agattagaaa gttttgctaa ctgatatggt cttctatcct tacctaacca ggtttctgtc
    11341 tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc taaaaaaata
    11401 cgttctgtct gaaaagtgac tgaccgaatg gggaaagaag ctgtttctct ctcaaaacta
    11461 gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag tatcgtcact
    11521 tctacatctt ggtcatctgc atagacagtt aataaaccaa caagtttgcc tttttcataa
    11581 taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag atagggatcg
    11641 cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc agatagctcc
    11701 tcttggctta acttgtttct tgcttgaatc atataggtat cctctacaaa ccagacgatc
    11761 tgtgactggc atctttagcc tgctcgagtt tattgacata atactcccgt ttttcttcga
    11821 cttcgtgaat aacaggctca tctttcttac catgaagacg gacaatcttg gccggaatac
    11881 cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca ccgaccttgg
    11941 cattttcacc aatttccaca ggcccgataa cttgggcatg ggctgatatg agggctccct
    12001 ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccgaga gtcactccgt
    12061 gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga ccagaaccat
    12121 ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc tgagtccaaa
    12181 agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gtcgtacttc cagagaaaat
    12241 gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa acctccaaag
    12301 tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgtgc caccacccca
    12361 tacatttctc cttttcttac tctgaatctt ttggtgtttc tgtaaattct ttcttaggtt
    12421 tgtgatcctt gtgatgacgt gggcggtgag ggcgctcaga cttttcacct ttttcatcat
    12481 gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata cggccttttt
    12541 catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa tcctctacac
    12601 gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc ttatcaaaga
    12661 ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa acttcatcca
    12721 ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata gcatcttggt
    12781 cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca cctgtttcag
    12841 cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc ttgtccacat
    12901 caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctggacga acttctggaa
    12961 tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga gcaagagcct
    13021 ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg gctgtaatcc
    13081 catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc aaaccttgga
    13141 tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca ataccagcta
    13201 ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca cagatagaag
    13261 cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata gcgtatggga
    13321 attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca ccgtgaccga
    13381 tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat tgagggaagt
    13441 tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg atttgagttt
    13501 ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga gtaaagagac
    13561 ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg atttcatcga
    13621 ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact tctgcgtgtt
    13681 ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct tcgtggttcg
    13741 catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt gcagcttcac
    13801 gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag gctgcaatga
    13861 tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt tctttaccga
    13921 cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg tgccctttaa
    13981 gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct accatgttga
    14041 tagcgtgctt ggttccagct actgtcaatt caagaagaga ttgctctgct tgttcttgac
    14101 ttgggttgat gatgatttgg ccatctacat atcccacttg taccccagca attggtccgt
    14161 caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca gccattggtg
    14221 cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca ttacggaaac
    14281 cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag gtcgcatctg
    14341 ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc gcatacattt
    14401 tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc ttagacataa
    14461 cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca tttgcttgct
    14521 tagcaacctg accagtttct acaattaact cacgacccgc aaaagtcgtt tgaaacactt
    14581 gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct acaaagatca
    14641 agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc ttcgatgaag
    14701 acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca caagatattt
    14761 tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg tcgcactcga
    14821 cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca agtcatcaag
    14881 ataccaagcc gtcaagcaac tcaaaggaaa ataggaaatc gaacgacgga gcgactgctc
    14941 ctagggagat ttatcttttt ccacagagtt gtaggcaagt tcagttttca agatacatca
    15001 ttagaaaggt ttaatactaa agtatctaaa gctttcacgc taatcgctat cgggcgatta
    15061 gctaaatgct ttactaactc tctcgtcaaa taacatcgat ttgactcact cgtgtcgtta
    15121 aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat caagtacgta
    15181 cagaatttat tttatcatat ttttcttaaa aagtgaggtc tttaccatta aaaaggaacc
    15241 attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg tgattaaaca
    15301 aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc gtatagagat
    15361 gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca taggcaagtc
    15421 ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct ttaaatttgc
    15481 gtggaaggtg gatattctca caagtcttca agagtcggag agcctgattt cgattcagaa
    15541 ttggcataat tcctgcatga atgggaacat caatcccagc caagatacac ttgtcctgaa
    15601 aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa cagcctgcat
    15661 ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct ggatgccctt
    15721 ctggatagca agctccaata atatcaaagt gaggggctcg ttccttgata agctcaatca
    15781 agtcggttgc atagcggaaa tccttttgtg gttccacgtc tggaataata tccccacgaa
    15841 gagccaagat tttctgcacc ccaactttgt ccaagtcagc aatagtttca gcaaccttgt
    15901 ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaaa tcattttgga
    15961 taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg ctggcagtta
    16021 cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata atgttaccat
    16081 tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt tggcgtgaca
    16141 tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg agagaaatcc
    16201 aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc aagctttctt
    16261 ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa actttcttgc
    16321 ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca tttggtacac
    16381 gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt ttcgctttga
    16441 gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg tctgcatcca
    16501 tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga gtgtggattt
    16561 gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc cagtcaaggt
    16621 aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca gcctcatcaa
    16681 tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg atagcaaggg
    16741 cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac cagttgagga
    16801 tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacggctt tgtgcatagc
    16861 tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg attggtggtt
    16921 tcaccccacg cataccgtat gactgtaccc aaccattttt agagaagagg taacctgaca
    16981 agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga acaaggacat
    17041 caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca aggaaagcgt
    17101 cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggta cgaacttctt
    17161 ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg aaagcttctt
    17221 cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg tctgtcaagc
    17281 cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca aagagttctt
    17341 tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag tcacggattt
    17401 catccaattt ttcaactgca aaagcaaagt ggttcaagag tgctggttca aattcttcat
    17461 tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg atgttttcag
    17521 ctgggatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag atgtttttac
    17581 cattgacaat acctgcatag agagtcttgt cagctgggaa gccaccttta acgagttcaa
    17641 gagttttctt accttcaaca aagtcaagac cgatagcatc tactggcaag tttacaaggt
    17701 cagcgtagac gtcacgaaca tcaccgaaat aagtttgaag caagacttca agaccttttt
    17761 tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca gctgtcaagt
    17821 cttttacaag agccgcttca tccaattgga tgcaagtcgc accaagttca gccaatttag
    17881 caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct ttcatgcctt
    17941 cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga cgagtgttca
    18001 atccaagttc tttggcttct tggaactcat caaaaatctt gtgaccagcc aatttcactt
    18061 gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac catttcttca
    18121 ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa gcgaagtagc
    18181 gctcaaggtc agacaagtcc aagttttgaa ctgaggcagg caccacgttg aaaaggaaag
    18241 ccgcatctag gaagttgtca tagtgagaaa agtcatttga tgggatttca gtgatgcctt
    18301 tttctttgac gatgttccag tgtttagcac gcaagtcttt tgcggctgca aggagttctt
    18361 cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattcg cggaattcgc
    18421 ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa tttgttgttg
    18481 aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg gtttgatata
    18541 gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa cgtctcaaat
    18601 cccttttata acttgttttt agctgtattt tagttagaat gctcaaacgc gttattagta
    18661 attcttataa gtgactatgg cttgttatta gaaaagacta taactgattc tagtcaactt
    18721 ttcccctgtt caagtggaac gactgctagt gtctttccta gactagctag gacttttaag
    18781 actgtgtcca actgtggact agtcttacct gtctccatcc tagctataac aggctgactc
    18841 actccactga gttcctctag ccttttctga ctgattcctt gcttatttct ggcttcaatc
    18901 aactcgctca tgatagctac tcgcatatca ctttcaagga tttcctcctt gctaaagagc
    18961 tcagatggac atccttccaa ttactcccaa tagcactatt cttcatcact tacccctctt
    19021 tcttttagat ctatgtattt tttgaaaaaa tgagcgaatt atgattcgat agattgatca
    19081 gcgggtttaa aattgggact aacttgtttc ttaagcgatt ttccttttct aggataaagc
    19141 aaatcctgtt tgcttaaccc caactttcca tgatgaatcc aatagtaaat ggttgaaatt
    19201 cccacgttaa cccctttagc catcaccatc atttcaggca aaaatttttg gttatgatag
    19261 tggagaatct tttcctttag ttccttggtc aagcttgatc tcttgaccga gtgcttgcga
    19321 ttattttcat aagactgttg agcatagtcg gcagaataaa tctctttgaa gcgccctttt
    19381 ccaagacatt ttcggactgt cccatacttg atttcagtat ggatagtttg aggagttttt
    19441 ccaagtagag aggcaatttc tctatttgat tttccttctt ttttccatcg ttcgattaag
    19501 cgacggctat cgattgtcaa atgtttgcct tttgtagtat aattgtcttg catttctgtg
    19561 ccttttaatc atttcaatct taaattggac tttttttact tgggttgtac ttaatctatg
    19621 aggaagacaa gaaaaagaat atcaatcaag taaagtcaca aagtcacatt agctccgagc
    19681 aaccattgca aattgaggta ctcacacaat gattaaaaca tttctctctg ccctttcggt
    19741 cattctcttt tctatcccta tcataactta ttcttttttc ccatcttcta atcttaacat
    19801 ttggctatct acccaaccta tcttggcaca gatttatgcc ttccccttag ctactgcaac
    19861 tatggctgct attttaagtt tcttattttt tttcctatct ttttacaaga aaaataaaca
    19921 aatacggttt tactctggca ttttgctctt actatcgctc atattactat tatttggaac
    19981 agataaaacc ctttcttctg catcaaataa gactaaaacc ttaaaattag taacttggaa
    20041 cgtcgctaat caaatagaag cacaacatat tgagcgaatt tttagccatt ttgacgccga
    20101 tatggctata ttccctgaac tagctaccaa tatcagaggt gagcaagaaa accagagagt
    20161 caaactattg tttcatcaag ttggactttc tatggccaac tatgatattt tcacttctcc
    20221 acctaccaat agtggaatag ctcctgggac tgtgattgtc aagaaaagtt atggtttcta
    20281 tacagaagct aaaacttttc atacaacacg gttcgggaca attgtattac attcgagaaa
    20341 acaaaatata ccagatatca ttgccttgca tactgctcct cctctgccag gtttaatgga
    20401 aatctggaag caagacttaa acatcattca taatcaattg gcttcaaaat atccaaaggc
    20461 tattattgca ggtgatttta atgcaactat gcgtcatgga gcacttgcaa aaataagctc
    20521 tcatagggac gcattaaatg tactgccacc ttttgaaaga ggaacttgga atagccaaag
    20581 tccaaaactt tttaatgcaa caatagatca tattttattg cctaaaaacc actactatgt
    20641 taaagattta gacattgtaa gttttcaaaa ctctgatcat agatgtattt ttacagaaat
    20701 cacattttaa ttattttata taaaatcacc cctctagtgt tcataaacta gagggggaat
    20761 ttgtatcata ctatcgttta acgcacttct gcattgactt tttcttcgag cgatgcttgg
    20821 attttttcca tatagcgtgc gacttcttcg tccgttaagc tatcttctgg attttggaag
    20881 gtcaagctat aagccattga cttcatacca agtcccaatt tctcacctga gaagacgtca
    20941 aaaagtttga tatctgtcaa acgtttcacg ccggcagctt ggatagcatc tacaacttct
    21001 tgatgagtca cttctgcctt gaggagaagg gcaacgtcac ggctgactgc cgggaatttg
    21061 gtgatttcta caaatggagc cgctggctga agcgcagctt cgatagctga aaggttaagc
    21121 tcagctacat acgtttctgg aatatcataa gccttagcag tgactggatg cacttggcca
    21181 aggaaaccaa gaacttggtc accgagtgaa atgactgctg tacgacctgg atgaaggcta
    21241 gcgatttcag atgttgctgt ataggttact tggagtccca aacgagcaaa tagggcttca
    21301 aggattccct tagcatagaa aaaatcaact ggaactgctg ctgtttggaa atctttttca
    21361 gcaaccaagc ctgtcaaggc aaaggcaaaa ctgttgattt catttggaag ttcttctttt
    21421 ggattacctg tttgttcaaa gacttttcca atctcgtaaa gggccaagtt tttattctta
    21481 cgagccacgt tgtaggcaac ggtatcaagg atacctgaaa tcatattttg acggaggact
    21541 gaacgatcca cagtcattgg ccacatgagt tcagtaaggt tactcggttg agctgtgaac
    21601 tcaactgctt tttcaggagt tgtcagagca taggtgatga tttctgtcaa acctgctcct
    21661 tcagcaatag tacgaacttg acggcggagt ttttgtgtgg ctgtcaattc acctgctgta
    21721 ccatcgtctt ttggtagact agttggcaag cggtcataac catagatacg agcaatttct
    21781 tcaaagaggt cagcttcgat tgtgatatcc caacgacgac gtgggactct gactgtaaag
    21841 ctgtctgcat ttccagaaag accaaagcca agacgacgga agacgtcttc tacatcagca
    21901 taagacagct cagttccgag gacacggtta acatcagcaa gggttgaaga aacttctaca
    21961 tctgaagtat caagctcacc cgctgaaacg atacccttac gcaccgtcgc acctgcaagt
    22021 tccgcaatca ggctagctgc cgcatcaagg gcttcattaa ctgttgccac attaattcct
    22081 ttttcaaagc gagaagatga ctcagaacga aggttcaggc gaccacttgt cttacggata
    22141 gttttgccat tgaaaacagc agcttcaagg acaacacgac tagatttttc agagatttct
    22201 gttgcttgac cacccatgac acctgcaagg gctactggct tgtctgcaac agtgatgact
    22261 aggtcattca cgtccaagtc acgttcttca ccgtccaagg tcaccaattt ttcaccagca
    22321 cgcgcttcac gcacacggat gtcagtccct tcaaatgtgt ccaagtcaaa ggcatgcatt
    22381 ggttgaccaa aatagagcag gatgtagttt gtcacgtcca ctacgttatt gatgggacgg
    22441 attccttcgt tcatgagaag gttttgcaac cattgtggac ttggtgcgat ggtcacattg
    22501 tccaagatac gagctgcata gtaaggcgcc ttgtctgtct caatgctgac agaaagggca
    22561 tctgccgcag cttcattagt ttctgttaga gtaaattctt taaagttgac tgccttgtca
    22621 tagatggctg ccacttcgtg agccactcca cacatagaaa gggcatctgc acggtttggt
    22681 gtgatggaaa gttcgatgat ttcatcatcc aagtctagat atgagaagac ttcctcacct
    22741 ggaacggcat tttctggcaa aatttgaata ccatctgcga attccttagg tacaactgag
    22801 tcagaaatac ccaattcacc aagtgaacag atcattccaa gtgactctaa gccacggatt
    22861 tttccttttt tgattttgta attgtcagcg atacgagctc ccggaagagc caccataacc
    22921 ttgatgcctg cacgcacatt tggggcacca caaacgattt gacgagcttc ttcttcgcca
    22981 atgttaacct gacaaacatg gaggtgagtt tctggcacat cttcgcaaga caagatctca
    23041 ccgacgacaa tttttgaaag accggcagct ggtgattcga caccttcgac ctcgatccct
    23101 gtggttgaca ttttttcagc caactcctgt gatggcacat caatgtccac caattctttt
    23161 aaccatttat aagatacaag cataatttag ttctccagaa tgaccattgc cacactggtt
    23221 cttttccttt cctatcattt caatagaaga attctcttct taccttaatt tctttctcag
    23281 taaccaatcc gtatctactt tttgaccaac cataaaatga tgttggctaa atttttcaaa
    23341 accatatcgg ttataaaacg cttgagcttt tgtattatgc tcccaaacac ctagccaagc
    23401 ccaagaaaaa ctattttttg tagcaagttc aagtgcgaat tcaaacagtt gcttacctag
    23461 tccaaatcct tggaattttt gtagcacata gaggcgttga atttcaaaag cgtcctctaa
    23521 ttctctctca gtttgagcac ttccccagtt gactttgaga aaaccagcta tctcctcctc
    23581 atgcataatg aaataggttt cagagtcagg atttcccaac tcagttgaca aagtttttag
    23641 actataagcc tcttcaaagt attcctgtaa ctgctcttcc gtattatcat acgcaaaggt
    23701 ttcacgaaag gcttgtttgg caattttagc caacacctca acatctgcca tttctacttt
    23761 tctaatcatt atttaaactg ttctgagaag cggacatctc cttggtagaa tccacggata
    23821 tcgttgattc cataacggag catagctaca cgctcttgtc caagaccaaa ggcaaagcca
    23881 gagtatacag tcgcatcgat accactcatt tcaaggacac gtgggtgaac cataccggcc
    23941 cccataattt cgatccaacc tgttttctta catacattac agccttctcc accacacttg
    24001 aagcaagaaa catccacctc aacagatggc tctgtgaatg ggaagtaaga tggacgcaaa
    24061 cgaatttgac gctcttcacc aaacattttt tggacaatca actgaagcgt tccttgaaga
    24121 tcagccatag agatattttt cccaactacc aagccttcga tttggtggaa ttggtgactg
    24181 tgggtcgcat cgtccgtatc gcgacggaag acacgccctg gcgagatcat cttcaaagga
    24241 cctttagaaa aatcatgggc atccatagca cgcgcctgaa ctggagacgt gtgggtacgg
    24301 agcaagattt cttcagtgat atagaaagta tcctgcatat cacgagctgg gtggtctttt
    24361 ggaaggttca tacgttcaaa gttatagtag tcttgctcca cttcaaaacc atccacgact
    24421 tgataaccca taccgatgaa gatatcttcg atttcttcac tggtttgtgt caaaacgtga
    24481 cggtgaccag tcgcaactgg acgacctgga agcgtcacat cgatactctc gctagccagt
    24541 tgagccgcga ctttcttttc ttccaagagc ttagctgttt cttcaaaagc agcagtcaag
    24601 acatcacgag cttcattgac gtgtttcccg atgattggac gcatctcagc agaaacatct
    24661 ttcatccctt tgaggatttc agtgagcgaa ccctttttac caaggacaga gacacgcaaa
    24721 tcttgcatct ctttttcatt tccagcagta atctgcttca agctagtcag cgttttttcg
    24781 cgaagcgctt ttaattgttc ttcaatagtt gacatatttc ctccatcagt ctctcgtaga
    24841 taaaaagaaa accacatgcc aaaaactcca ctcggagcgt tgacacgcgg taccatccgt
    24901 tttcatctga caagtcagac cttcatttct aaatccatgc gcaagtgaat tcacccagct
    24961 ttcatataga gagcttgcag tcacggctct cctccctgat atacttccct tgggctacta
    25021 gtctttcaga ttcctattca attactactt agtttatcag atttttacca ttcttgcaag
    25081 acctatctta cttctgcttg ttagcttatt cttatctaaa tttatataaa caatttatct
    25141 aaattaacta tttataattt ttgtaacaaa attaaattaa ttgacactcc cctataaaat
    25201 aaagaagttt agaatttaat gtcttccaaa cttctttatt ccatattcaa tgagatgttg
    25261 ccttaaccgt gataatatct aatcatcaat aaacaaacta tttgaataag gattctccat
    25321 ttgattcaat cactccttta taccaagtaa aggacatttt cttatatcga ttgaatgtgc
    25381 catttccatc atcatttcga tcaacataaa tgagaccgta cctcttagaa agctgtgcag
    25441 tggacataga aacgcagtca atacatcccc aagacgtata gcccataatt tccacaccat
    25501 cttgtagagc ttcagctact tgcaataaat gttctttcat atactgaatt ctataatcat
    25561 cttggactgt taagttatta aattcatctt ttattagttg atctttagca cctaatccgt
    25621 tttccactat aaataatgga atttgataac ggtcataata tctatttaaa attatacgta
    25681 gtccaattgg atcaatttgc catccccact cagaagactc taaataagga tttactaagc
    25741 caccaataat attcccttct cctgaattat actgtgttgg aagagcagat tgagtcacac
    25801 tcatgtaata gctaaaggat aaaaaatcta cggtataatt ttttaataac tctgcatctt
    25861 cagctgcaaa ctctatgtta atgtcatgtt ccttaaaata tctttttgca taattcggat
    25921 aataacctct aacatgcaca tctgaaaata gataatttag attctcatac tcatgagtcg
    25981 cccatacatc ttttggattt ggagtcattg gataagctgg catagctaat accatacacc
    26041 ccaccttaaa ctctgaatta atctcacgag caatttttgt aaccaaactc gaggcgacta
    26101 attcatgatg tatagcttga tataattctt gtttcgaaag attctcctta ggtatatcta
    26161 ttcctccact agtaaacggt aattccaaaa cagagtttac ttcgttaaat gtaagccaat
    26221 atttaacttt atctttatac ctttttaaaa ttgttcgagc aaatttttca taaaaatgaa
    26281 tcattctcct atcaatccat ccatgatatt ttcttgctaa atataatgga gtctcatagt
    26341 gtgaaagagt tacaagtggt tctatcccgt gagcatgtag ttcatcaaat aattcatcat
    26401 aatatttcaa accagcttcg ttaggttctt cctcatctcc ttttggaaaa attctactcc
    26461 atgcaataga agtacgaaaa acattaaagc ccatttcaga aaacaaggat atatcttcct
    26521 tatatttatg ataaaaatca atacctatca attttaagtt atcttctgta ggattttctg
    26581 ttgcttctcc taatccacct ttgggtaaca catcctgaac tgataagccc ttaccatctt
    26641 cattatatgc tccctctact tgattagctg caacagctcc accccaaaga aaatcatctg
    26701 gaaaaatggt cataactttc ctccattata atattaccag taattcctta gaatgctcga
    26761 ttgtctgatt attaggtaat actaatacat ctagaaaatc attggtattc gttacaatta
    26821 ctggtgtaac tgtttcgtag cctttagtct tgattaaatt caagtccatt tcaaaaatca
    26881 actgattttt gaaaactctg tctccttctt ctacatgact aataaaacct tgacctttta
    26941 gctcaacagt atctaatcca atatgaatta gtaactcaac accctcatca ctcttcaatc
    27001 caattgcgtg cttagtcgga aaaatatttg taattttccc atcaaatggt gcataaacct
    27061 taccttcact tgggataatc gctactccgt ctccaattag tttatctgaa aatgttttat
    27121 cctggacatc gcttaacgga atgatttctc ctgatatagg agaaaatatc atttttttat
    27181 ttgaaactcc agctttaact tctaaattac tagaactctc ttcttcatcg attccaaata
    27241 tataagctaa tacaaaggta ataacaaccg aaatgaccgc cacaattaaa gcatttacaa
    27301 tatttgatgg tacatcagaa taaataaatt gaggcaacgc tatcaaagat gggacagcaa
    27361 atagatacgc tttaacacta gtaagacctg caaataatcc cgctaatcca ccaccaatca
    27421 tagctgcata aagcggtttt ttatatttta aagtcacacc atataatgca ggttcggtaa
    27481 ttcctgcaag taaggctgag aaacctgctg caaaagcaat ttgttttgta ttattatttt
    27541 tactctttaa tgcaacagcc atcgaagcag ccccttgagc taagtttgac cctaacattg
    27601 ctggaagaat taatacgtct ggagtagcaa tagatgccgc caaaaaaata ggtgcaaaag
    27661 cccaatgcat tccagtcata acaataaatg gcataatagc accaagaata gctaatgtaa
    27721 gccatccagc tacaccatac atttgcccaa ctagatttga taatccttca ccaacaatta
    27781 ctccaatagg tccgactaca actaaggcaa tacagcttga tactaataat actagcgtag
    27841 gttgcaaaaa actcttagta acagctggtg ttaatttagc aatcattttt tcaatatatt
    27901 tcatcaacca aaccataata agaattggaa cgactgacga accataacta gctggtgtca
    27961 caggtacacc aaataaacta agaggattcc ctgattgcac catttgaaca aaatttggat
    28021 ggagaagtac acctgctaca gacatagcta atgtagatgt tacttttaat ttttgtgatg
    28081 cagaataagc taataacagc ggtaagaaat aatatggcgc atccccaaaa aatgtcaaaa
    28141 aagcaatagt ctgagaatct gattgcaata taccaagcat tggtaaaata attaccaaga
    28201 ctttcagcat acctccccct aacattgctg gaatgattgg agtcatggaa ccagcgatat
    28261 actcaatgat tctttctaaa atattccctt tgtgcccttg aacaactgaa tcggattcaa
    28321 aattgccaag tttaacgaat tctttataat aattagctac atcattacca agtataattt
    28381 gatattgtcc attctttttc ataataccta ttacacctgg tatcttcttc acatcatcat
    28441 cattgactaa attttcatct tttaattcta atcttaaacg tgttacacaa tgggtaactc
    28501 tattgacatt tttttcgcct ccaattacat cgaggatttt ttgtaccgta tctttataac
    28561 tcatggtatt ctcctattct attaatctaa attttttgtt aagcgacgaa tatgagccat
    28621 caaataaact aattcactag aagtcagcaa ataattgtac tcagtttgta taaacattgc
    28681 cacctgttca ccacattcat attctccagg atatttattt ttcattaatg ctaacaagtc
    28741 ttcatcatca tcgtcacaat aagttgtatt ttcaaccaag cgatgggcaa ataattttat
    28801 atgagttatg aatctataat aatcaataga atcttggtta aactcagtac aatagaaatc
    28861 ttgaatgatt ttctcaattc ccataattat ttcagtgatt ttatacgact cttgaaaatt
    28921 attttctaaa ttagcattaa caaaatgtag cgctatgaat gcagcttcat caattggaag
    28981 ttcaatacct aactcttttt ttattatttg taaagccctt ttcccaagtt caaattcttc
    29041 tttataatat ttcgatattt cccagcgtag gggattcgaa ataataatcc cttctttata
    29101 acgttctatg ctcgaagaaa tatgatccgt taaattaata tagataattt catcaaaatt
    29161 attacccaat gttattttac ctaggttaat tattttttcg gtgatgtaca ccaactccaa
    29221 aggaacgtta ataaacatct ctgtaaaacg ggtcatatta tctctatttt tgagaaaaaa
    29281 acttttctca atatcaattg gatttacttt atctccagtt tttcttccaa aagctattcc
    29341 ccttccaaaa agaagaatat cgatatcttt tttactttgc gcaattacgg cattgtggtt
    29401 tagtattcgt tttatgatca taaaatttcc cttttaaaat aaaaggaacc tacttaaaaa
    29461 catcaagaaa cttcttccga tgtgattaaa taggttcctg ctcacttgta ttagtgataa
    29521 catcctttta tttcaatttt taatggtgac atgaagctta aatcattaat aactctgtaa
    29581 ccattttcat tttaagatat ttcaatctat ttgtcaagta cttgtagaaa tttttttatt
    29641 ttgtgtgatt cgaatgtgat aatggcaaca gtctcactaa agaaatgaaa atcatacata
    29701 gaaattttta aaaattttct ctatcgtaaa cctgccacta gttcagaagc tatactagct
    29761 gattccaaac gtaatatggt catctcatct accttcccta ataatggcat tgcaccatac
    29821 caaacaatag tcttattcac caaaagaata ggcgtcacta tttttgattg agtaaaagac
    29881 actttaattt tctggctctt caactggtct agccatgctt tatttgcaac cgtctctggt
    29941 atacatatct caatttgact aatcctaaat tcttttagaa aattcatcag ttttgtctgg
    30001 tgcacataag gtaaaataag cagacattcc tgtctttccc ctgctaagtc cttcctcaac
    30061 acattctcat atctactatc aacataaacg aattgtttct cctcaccctc aatggcacga
    30121 taatccatct ttcgataagc tacttgtcgt ttctgaaaca tcttttctaa ataaggaaca
    30181 tgaatatcca cataatcgaa aatacgtacc aaagacttat ctttgtagtt tctatgaatc
    30241 cgaccggcgt actgaatcaa attatttttc caagaaaagg gcgctgccaa gatcagcgtg
    30301 tccaactgag gtaagtcaaa accttcgcca atgtattttc cagttgacaa caatacaaac
    30361 cctttatcta gctgttctaa tatctccagt aaacttgttc tctctcggac tttggttttt
    30421 ccgctaataa tgtaacagcc atcaacctct ttctctttca ataacttttc aaaaacatct
    30481 atctgttgaa ttcgattaac tagaaccaag atattccgac cttctgccac ttgggctaga
    30541 atatccttga gaatcaactg attcctcgct gagtctgtag caatccaatc actgagctgt
    30601 ataaaattac ttgctttggt cttttcaatt tccaaatgag caaaagaagt gaatcttaat
    30661 tgcaattgcc gtttaaaatc cgtttccctc ttatcagcag tatggagtat ctcaccaatt
    30721 ctctgaaaaa caataggctc atgaccattc ttacgctcag gcgtagccgt caaaccgtaa
    30781 agatacttcc ctctaaactg agcaacaact ttttcaaaca tcaaggcaga gacatgatga
    30841 cactcatcca caatcatcat ctcatactca tccaaaagac tttgactatt ttccaactta
    30901 aatagagatt gaatcataac gacatcaacc agtttactca gccatttctt agtcccaccg
    30961 tactgtccaa catagccgat taccttttca cgacctgatg ccgtataacg gatagtctcc
    31021 tcctcttcga aagtcaaaaa gcagtttaag cgatctagcc attggtctaa gagttgctta
    31081 ttatggacta gaataattgt ttttgttttc cgttcagaga taagagcagc acctaaaacg
    31141 gtcttcccaa aacctgtccc gcatgaagta aaccattttc tttagaattc atatctgaca
    31201 gggctaattc ttgctccaaa gtaagctctc ccttaaatgc tactctaata gacctttgta
    31261 cttttctcct atcctccaca actacctgct taaatttatc ttgcaatgga tataacaagc
    31321 ctcttggaag ccataaataa taatcggatt ctccaaataa atacattcgc tcaggaattt
    31381 gataggttgg ctgtcgcata gcctgcttta aatatagtaa aatgaaataa taacaggaca
    31441 aatcgatcag gaaagtcaaa tcgatttcta acaatgtttt agaagcagag gtgtactatt
    31501 ctagtttcaa tccactataa aattcgggat tagaaaagga agccatattt ttcaagaaaa
    31561 aaagtgcctt ggatgtcaca gacgattttt caagttggat catattggat aaaacgacct
    31621 tcaactcctt atccaattct tgcttgccta actcctcttg gattaacagt gccactttag
    31681 cagttgaaat cctctgaatt tcttgtagat acctccattg atcttcataa ggctgaaaat
    31741 gttcatccac aaagaccgtt cgcccttgat gataagcttc tccttgaaaa ggaagggaaa
    31801 ttaaattatc aaatccacct ttaggaagaa catcctgatt tggaaacatg ccatcaaaag
    31861 aatcaaagga cagttgcata ctttcctgca tagccaattc taatagtttc tttccaaaca
    31921 ggcgagcctt tcgactcaga atcgcttcct caaagaagaa ccaaatatgg agtccgtaac
    31981 ccgaacgaga aatctctaaa tgggcttcca tctggcgttc cctggctatt cttcgaatgg
    32041 ttaaaccagc ttctttccaa tctccttcat caaaatccag taccaaaaaa tgacagctat
    32101 catctaagtg cataggaaag ataccgatag ctgtctcccc acgaaaatga gatttcaaaa
    32161 cggaatccgt caatgtctgg aaactccgtt tttcaggtag caactgcttc caaccataat
    32221 cataggaagg aaaatactga attttccctt gttcattgat aaaattctta gcataaacat
    32281 cataacgtcc agcaaacacg gaggtaaaca attgtgattt ttcttgcgtc gtcaagtgac
    32341 tactttgaag ttggaacatc tcctcgaacc gagagcgttc catcacaaac tgactcttgt
    32401 ctttaagacg tttacagtgg aatgaaataa gatatgaaca aattgattag gaaaatcaaa
    32461 ttaatttata gaaatctttt agcagtcatg gcgtcctatt ccagcttcaa aatgctatac
    32521 acaagaaaaa ctcactatcc ccattaaaag aaaaaaagac atcaagtatt tctaccacaa
    32581 ttccatccaa agacacaaaa acagccataa gagtcacctc cttgattcct ataggctgat
    32641 tataacaaga atagctgaaa ttgtacacga aaataaaatc ctaatagtac tcattttgta
    32701 tgtgactaat attccgtctc gctccagaag gtacgaagta aatagagttt aaacgagcag
    32761 tctataaaat tcaaaaaacg catattatca agcgacctaa aactttgggc tctataatat
    32821 ttgtagtggg taaatccact atagatatta tggagcctat tttattgtag aaaaaaatcc
    32881 cataagatct ataatgaaaa gtgaccaaac aactcattag aaagattcat atgggacaat
    32941 tacattttat cacaaaacta ctcgatatca aagacacaaa tatccaaatt atagatgtcg
    33001 tcaataggga ttcacacaaa gaaatcatcg ccaaactgga ctacgacgcc ccatcttgcc
    33061 ctgagtgcgg aagtcaa
//
LOCUS       DAVDWM010000019        32069 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVDWM010000019 DAVDWM010000000
VERSION     DAVDWM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.019"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(298..480)
                     /locus_tag="VUW49_001089"
     CDS             complement(298..480)
                     /locus_tag="VUW49_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981017.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(482..1261)
                     /locus_tag="VUW49_001090"
     CDS             complement(482..1261)
                     /locus_tag="VUW49_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981018.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            complement(1540..1890)
                     /gene="rbfA"
                     /locus_tag="VUW49_001091"
     CDS             complement(1540..1890)
                     /gene="rbfA"
                     /locus_tag="VUW49_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7981019.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(2141..4933)
                     /gene="infB"
                     /locus_tag="VUW49_001092"
     CDS             complement(2141..4933)
                     /gene="infB"
                     /locus_tag="VUW49_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7981020.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(4950..5249)
                     /locus_tag="VUW49_001093"
     CDS             complement(4950..5249)
                     /locus_tag="VUW49_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET7981021.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(5242..5535)
                     /locus_tag="VUW49_001094"
     CDS             complement(5242..5535)
                     /locus_tag="VUW49_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET7981022.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(5557..6693)
                     /gene="nusA"
                     /locus_tag="VUW49_001095"
     CDS             complement(5557..6693)
                     /gene="nusA"
                     /locus_tag="VUW49_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET7981023.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKIGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(6737..7216)
                     /gene="rimP"
                     /locus_tag="VUW49_001096"
     CDS             complement(6737..7216)
                     /gene="rimP"
                     /locus_tag="VUW49_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET7981024.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(7342..7977)
                     /gene="trmB"
                     /locus_tag="VUW49_001097"
     CDS             complement(7342..7977)
                     /gene="trmB"
                     /locus_tag="VUW49_001097"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7981025.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(7974..8768)
                     /locus_tag="VUW49_001098"
     CDS             complement(7974..8768)
                     /locus_tag="VUW49_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7981026.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(8929..9540)
                     /locus_tag="VUW49_001099"
     CDS             complement(8929..9540)
                     /locus_tag="VUW49_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7981027.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGVFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLTYL"
     gene            complement(9570..9818)
                     /locus_tag="VUW49_001100"
     CDS             complement(9570..9818)
                     /locus_tag="VUW49_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981028.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            complement(9860..10549)
                     /locus_tag="VUW49_001101"
     CDS             complement(9860..10549)
                     /locus_tag="VUW49_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7981029.1"
                     /translation="MKKYQLLFKTSAILSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(10581..10814)
                     /locus_tag="VUW49_001102"
     CDS             complement(10581..10814)
                     /locus_tag="VUW49_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HET7981030.1"
                     /translation="MDTKMMEQFEIMDTEMLACIEGGDVSGIYRGYAYQDSPFGPYPS
                     ILKNSDPFPVSGYCPRGYRDLGYIGAGFHLCGI"
     gene            complement(11061..12030)
                     /locus_tag="VUW49_001103"
                     /pseudo
     CDS             complement(11061..12030)
                     /locus_tag="VUW49_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12191..12562)
                     /locus_tag="VUW49_001104"
     CDS             complement(12191..12562)
                     /locus_tag="VUW49_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981031.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQIMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLIIILFLIWGLKYYKK"
     gene            complement(13031..13150)
                     /locus_tag="VUW49_001105"
     CDS             complement(13031..13150)
                     /locus_tag="VUW49_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET7981032.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTP"
     gene            complement(13254..13403)
                     /gene="blpO"
                     /locus_tag="VUW49_001106"
     CDS             complement(13254..13403)
                     /gene="blpO"
                     /locus_tag="VUW49_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET7981033.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(13647..13931)
                     /locus_tag="VUW49_001107"
     CDS             complement(13647..13931)
                     /locus_tag="VUW49_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET7981034.1"
                     /translation="MGTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(14000..14269)
                     /gene="blpJ"
                     /locus_tag="VUW49_001108"
     CDS             complement(14000..14269)
                     /gene="blpJ"
                     /locus_tag="VUW49_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET7981035.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLVGAFVGAHIGAIGDGLTCLGGMVGDKLGLSW"
     gene            complement(14736..14933)
                     /gene="blpI"
                     /locus_tag="VUW49_001109"
     CDS             complement(14736..14933)
                     /gene="blpI"
                     /locus_tag="VUW49_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET7981036.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            15214..15801
                     /locus_tag="VUW49_001110"
     CDS             15214..15801
                     /locus_tag="VUW49_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET7981037.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            15728..17371
                     /locus_tag="VUW49_001111"
     CDS             15728..17371
                     /locus_tag="VUW49_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET7981038.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHIFINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILKACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRL
                     SIAEQTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            17382..18743
                     /locus_tag="VUW49_001112"
     CDS             17382..18743
                     /locus_tag="VUW49_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET7981039.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTSNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            18800..18928
                     /gene="blpC"
                     /locus_tag="VUW49_001113"
     CDS             18800..18928
                     /gene="blpC"
                     /locus_tag="VUW49_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET7981040.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(18999..20339)
                     /locus_tag="VUW49_001114"
     CDS             complement(18999..20339)
                     /locus_tag="VUW49_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7981041.1"
                     /translation="MYIFWIILYTLITNGLEIVIFFKVDGIGLTFERIFKAFLLKILL
                     AFVFVMIGYMVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQEQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRREILDTGFQK
                     SLTKINWIMGAYYLVIQSLSYFEYEQGIQSTTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNVRDRALKSLLAGKFIKAREKDIVFNVEVPEEI
                     QVEGMRLLDFLTIVSILCDNAIEASAEAGQPHVSIAFLKSGAQETFIIENSIKEEGID
                     ISEIFSFGVSSKGEERGVGLYTVMKIVESYPNASLNTTCQDQVFRQVLTMIPTE"
     gene            complement(20352..21089)
                     /locus_tag="VUW49_001115"
     CDS             complement(20352..21089)
                     /locus_tag="VUW49_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981042.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(21094..21432)
                     /locus_tag="VUW49_001116"
     CDS             complement(21094..21432)
                     /locus_tag="VUW49_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981043.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            21709..22038
                     /locus_tag="VUW49_001117"
     CDS             21709..22038
                     /locus_tag="VUW49_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET7981044.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(22206..23255)
                     /locus_tag="VUW49_001118"
     CDS             complement(22206..23255)
                     /locus_tag="VUW49_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981045.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(23252..23983)
                     /locus_tag="VUW49_001119"
     CDS             complement(23252..23983)
                     /locus_tag="VUW49_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981046.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            24051..24461
                     /locus_tag="VUW49_001120"
     CDS             24051..24461
                     /locus_tag="VUW49_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET7981047.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            24471..24758
                     /locus_tag="VUW49_001121"
     CDS             24471..24758
                     /locus_tag="VUW49_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981048.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            24920..25204
                     /locus_tag="VUW49_001122"
     CDS             24920..25204
                     /locus_tag="VUW49_001122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981049.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDTTDEVSSKHCFEVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(25526..26662)
                     /gene="dnaJ"
                     /locus_tag="VUW49_001123"
     CDS             complement(25526..26662)
                     /gene="dnaJ"
                     /locus_tag="VUW49_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7981050.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGGLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(27064..27420)
                     /locus_tag="VUW49_001124"
     CDS             complement(27064..27420)
                     /locus_tag="VUW49_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HET7981051.1"
                     /translation="MSVLAEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIECLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSALVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(27422..29245)
                     /gene="dnaK"
                     /locus_tag="VUW49_001125"
     CDS             complement(27422..29245)
                     /gene="dnaK"
                     /locus_tag="VUW49_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7981052.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGNDVVDGEFTEK"
     gene            complement(29725..30249)
                     /gene="grpE"
                     /locus_tag="VUW49_001126"
     CDS             complement(29725..30249)
                     /gene="grpE"
                     /locus_tag="VUW49_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7981053.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(30276..31310)
                     /gene="hrcA"
                     /locus_tag="VUW49_001127"
     CDS             complement(30276..31310)
                     /gene="hrcA"
                     /locus_tag="VUW49_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7981054.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVVLEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(31474..31983)
                     /locus_tag="VUW49_001128"
     CDS             complement(31474..31983)
                     /locus_tag="VUW49_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET7981055.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         9728 a         7043 c         5578 g         9720 t
ORIGIN      
        1 aacatatata ctgcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaaa
       61 tgattttact gcttatatta taccatatgg agagagatta aaaaactagc agtggagttc
      121 ctgctagctc tctgcgactt ggattaccat ctagataagt ttaaagagcc aatccaatat
      181 cttaaataat atcttgtaaa acagcaattg aactgcaaaa gagatgatca tccaaaagaa
      241 tttcacaatc ccaataatcg gtttgataaa aagaatagca agaagaccaa aaaaatttca
      301 ttctctctcc tctggcttaa tgagccaccg agccgtatcc attagcttat ctgcaataaa
      361 aagtttgccc agaccaacaa cgacatggtc agctttcttt atcgttttca tcacttttac
      421 accttgcacg gtcaaaaagt aattaccata aattttagca aaagctttga taggattcat
      481 attactctcc tttgatgatt tcagcctctt ttcggattgt ttcaacatct tcttgatatt
      541 gaacttcact atagttgact agcttggata attctttctg caagctttcc cccattggtt
      601 tcatagttgc aaaggttaaa ccacctgaaa tgataccacc caagatagga ataaatttcc
      661 ctattccttt ggccagccct cccttggtaa gattcacacc aaatattctt aagacttttt
      721 tcaaaatagg gtaccaaagc gtctttgtta aagctttatt aggcactgtt ttcattacct
      781 gtttggcaat tgttatacca ccagcacgta gcaaagcagc ggttccattc acccctaaca
      841 ttacgcctag ataaagcaag agggtatttt gagcatcttc actcaactcc tcgcgtgaag
      901 cccaaagatc ctcataacca taaatataac ctaattcttg agccaatttc agagagaaag
      961 cataaaattg agccacatca gctggaatgg taattgccat agcaagccct ccagctaatc
     1021 ctgccaaaac agaagtccca ctcgctaata atacattgtc ccgaatacaa gcattagcta
     1081 cacgatctaa tatttcttga gatagaagag tcgttgtacc ttgttccaat aaggtaggaa
     1141 tatcttttgg atccaattct ttggaaaact tatccactaa aaatttcgaa cgatcaacct
     1201 taacaacagg tagtttcacc acttgttgca atacttgcat agctaagtct tgttcagcca
     1261 tatacaaact ccttatttct tttttacacc ttatcatatt atatttccta caaaaaaagc
     1321 attttctaat tagataacat taaaaaatac agaacccctt ttttatagta aaatgaaaca
     1381 agaacaggac aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga
     1441 ggcgtactat tctagtttca atatatttta aaagaatatt tttccatttc aaattcaacc
     1501 tattttcctc caccaaaaaa gaggggcaac ccctcttctt tagttcttat ccagattgcg
     1561 tagcatctcg tcaatcttgt ttccatactc gatggactcg tctttgacga aggtcaaatc
     1621 tgggattttg tacaatttca aattgcgacc aagttcacgt ttgatggtac cagttgcttt
     1681 ttcaagcccg atttgggctt tttggttatc cgaagcaagg ttactcaaaa tggtgtaata
     1741 aaccttggca acagacaagt cacccagcat ctgaacatct gtgatggtca caccttggac
     1801 acgtggatca cggactttct tttgcaaaat ctcattgact tcacgcttga tttccatgcc
     1861 cacacgatct gtacggaaat gatttgccat gatattcctt tctctacttt gaaccaaaag
     1921 ctaggccagc agacctagct attttaaact tcttgatttt cagtttaaat tgaaacgaag
     1981 ttcaatttga agtcatctgc ttctttcgcc gtggtgaaag tgatggaact gatttatcag
     2041 tcccatcaca ggggattttt gagaccctag gctcaaaaat aagcaatgaa accatcggtt
     2101 tgcttgcgtc cctcaccacc taagaaagga gcaaaaaatc ttatctcttg atttcttcca
     2161 tgacatacgc ctcaatcaca tcatccatct taatatcatt gtagccgtcg atcatcaatc
     2221 caccttcacg accgtttgtc acttctttca cgtcgtcttt atagtgtttc aagcttgcga
     2281 gttcaccatc atagataacg acaccatcac ggataacacg gactttagag tcacgggcaa
     2341 ccttaccgtt gataaccata aatccaccga tagttcccac tttagacacc ttgaaggttt
     2401 cacggataac cgcttcacca ataacttttt cttcaaattc tggatcaagc atccctttca
     2461 tagcttcttc catctcttcg ataaccttgt agataatgct gtgaagacgg atttccacat
     2521 cgtcagcttc tgcttgttga cgagcttgtg gtgtagggcg tacgttgaaa ccaacgataa
     2581 aggcatttga agcttcggca agggtcacgt ctgattcgtt gatagcaccg accgctgagt
     2641 ggacgatagt cactttgaca ccttccacgt caatcttttg aagtgaggca gaaagggctt
     2701 caacagaacc ttgtacatca gccttgatga taacattaac agatttgagt tccccagctt
     2761 taagggtatc aaagaggttt tcaaggctaa cacgttgggt agcttgacgt tgtttcatga
     2821 gggcacgttt ggcacgctct tcacctgctg cacgcgcaga tttttcatcc tcgtaaacgg
     2881 caaagtggtc acccgccatc ggtgcttcgt tcaaacctgt gatagagact ggtgttgatg
     2941 gtccagcaac tttaacacga cgaccaaggt cgttggtcat agcacggaca cgaccgaagg
     3001 tatttccgac aacgattggg tcttgaacat tcaaggtacc ttgttgtaca agaagggttg
     3061 cgaccgcacc ttttccttta tccaagcgcg cttcgataac cgtaccgatc gcacgaactg
     3121 ttgggtctgc tttgagttct tggatttcag ccacaagaag gactgtttcc aacaattctt
     3181 cgatattttg gttgaattta gccgaaattt caacaaattc agaatctcca ccccaagcag
     3241 ttgacatcac accatgctct gccaattcac cgataacgcg ttctgggtta gcacctggtt
     3301 tatcaatctt gttaatagct acgatgattg gaacgttagc tgcttttgag tggttgatgg
     3361 cttcaatagt ctgaggcata accccgtcat ctgccgctac gaccaagatc gtaatatcgg
     3421 taacagaagc accacgcgca cgcattgatg taaaggccgc gtgtcctggt gtatcaagga
     3481 aggtaatctt cttaccattt tccacgattt ggtaggcacc gatatgctga gtaataccac
     3541 ctgcttcacc tgtcgcaaca cgtgagttac gaagagtatc caaaagggtt gttttaccgt
     3601 ggtcaacgtg tcccatgata gtaacaactg gtggacgctc aaccaattca tcttcattga
     3661 gataaccatc ttcgacaaag aaacgttcga tgtcagcatt atccacttca accttttgtt
     3721 tggcttcgat accgtaatcc accatgagga gttcaattgt ttccccatcc aaggattggt
     3781 tttgtgtggc catgacaccc atcatgaaaa gtttcttaac gatttcagct ggttcacgtt
     3841 tgatacgttt tgcgatttcc gcaacggtca taccatctgt atattcaaat tctgttggca
     3901 attcatggaa tttacgctcc gtaacaggtt ttggagtctg attacggttg ttcttgttat
     3961 tgcctttttt gttctttttg ttgttattcc agttactatt cttttgattt ctcacttgat
     4021 tttgactact tcgattcttt tgttgttttc taggaccatc ttcttcatga tcataatcgt
     4081 cacgattttt gtctggtcga gcttgttttt tacgacgtgt atccactgca ggttcttttt
     4141 tctcagggac gacttcaacc actgcttgaa cttgctgtgc ttgttcagct aacttagccg
     4201 cttcttcaaa gatttcctct ggttccttgc gtttgttagc ttgagccaag gcttctttag
     4261 cagcctgata ctgcttgaag cgttcctcac ttgaacgagc gtactctgca ttttgctctg
     4321 cttttagggc tgctgcacgg gctttaaagt caatacgtgg agccacttga tttgaacgtt
     4381 tgtcctcttg ctgacggcgc tcatttctac gtttttgctg accttgctgc ttcttagctt
     4441 ggtcattaaa gcgacgattg tcgcggttgc tttgaccttg ttttccacca ttacggccgt
     4501 cgtttttctg acggtttccg ttttgttgtt ggtcacggtt attgccctta ttttgcttgc
     4561 gtcgttctgc ctgctcttta gcacgggctt cacgctcagc cttgaaatta cggctttgcg
     4621 gtttcgctgc tactttttct gttttcggag caactggctc agccggtttt gcctcttcct
     4681 tagctacagc tggtttagct ggctcagatt tttcggcttt cttttctgca cttacttttg
     4741 gtgctgcagg ttttgcttct actttcggag cagctgcagg tttaaagctg gcagcaattt
     4801 ttgcagcgac agcttcttcc acacttgatg agtggctttt cacatccaag cccaactctt
     4861 ttgcacgcgc tacaacttct ttactttctt ttccaagttc ttttgcgatt tcgtacaatc
     4921 ttttcttaga caaatcatgt cctcctcttc tattccataa gagacctcat tttctttgta
     4981 aatccagcat ctgttacagc caaaaccttt ctcgatttcc cgactgctat gcttaattcc
     5041 agtgttgaaa acacggttac aatttctact tgataataat gacttttatc ttgaatcttc
     5101 ttggtcagat tgggtccagc atcatgagct agaaagacca acttggcctt gccgtcttga
     5161 atggccttga ccaccaattc ttcacccgat atgatgcgcc ctgctcgctg agcaagcccc
     5221 aagagattac ttatcttttg cttattcaag tcccaactct cttcttttca ctttgtgatc
     5281 cacataagcg atcaactcgt cataaaagct ttcttccact tccatgctaa agctgcggtt
     5341 aaagaccttc ttctttttcg cctctagggc ttctgcattg tctagtttga tataagcgcc
     5401 gcggccattg gccttgcccg taggatcaat aaagacttgt ccttccttgt tcttgacaat
     5461 gcggagcaaa tcacgcttat caatcacttc gttagacaca acagacttgc gcaaagggat
     5521 ttttcttgtt ttcatctttc cctcctctag cagcttttat tcttctacag tatcgttttc
     5581 tacttccaac tctactgaag cagcgtcttc catggcttca aattcgctag cagacttgat
     5641 atcgatacga taaccagtca agtgagccgc caagcgcacg ttttgtccac gacgaccaat
     5701 ggcaagagaa agcttattat ctggaacaac caccaaggca cgtttgctgt cgttttcatc
     5761 aaagataact tggtcaacct cagcaggagc gatggcattg tagataaatt cagctggatc
     5821 tgctacccac tcgataacat cgatattttc ttcgattggt accatgcggt catttttagc
     5881 atcgtaacga gctgggtgga atttgctagt aatcttcttg atattagcac caccacgtcc
     5941 aacgattgta ccgatagcat ccacgtttgg attgtggcta cgaacagcaa ccttcgtacg
     6001 gtcacctgct tcacgagcca cactcatgat ttcaacagtt ccatcataaa cttctggaat
     6061 ttcttgctcc atcaaacgtt tgatcatttc tggatggcta cggctaacaa agacgttcac
     6121 accacgaggg ttgtcttcaa ccttgtaaac ataaacttcg atacgatcat gagaagcaaa
     6181 aacttctcca ggaatttggt cttgttttga caattgggct tcgatgctac caagattgac
     6241 atagataaag cggttgtcaa agcgttctac tgtaccagac atgatttctt gctcatgttc
     6301 tttgtaagta ttgtaagtga tggcacgtgt ttgcttgcgc attttttcca tgatggtttg
     6361 tttggcagat tgggctgcta cacgaccaaa ctcagctggt gcttcttcaa acttgatttt
     6421 gtctccaagt tcataagctg aattaatggc aagagcatct ttcaagctga tttccaaacg
     6481 gctatcaaat acttcatcaa caacttcacg gacagtataa actgtaaagt cacctatttt
     6541 ttcgttgaag tcaatagcta cgctgtctga ctgaccatag cgtctgcgat aagcggaacg
     6601 aagcgactct actactgcgt cgatgatatc ttcttttttg attcccttgt cttcttccaa
     6661 aatgcggaag gcctctagca tttctttact catattttct tcacttttga atcctcaaaa
     6721 gctatccttt ctttttctat aatttaactg ctaaacgtgc ttttgatact aaactgtatg
     6781 gaatttggac ggttttctta cgcgtcttgt ccatatattc catagtcaac tcgtcctctt
     6841 cgaaggccaa caaggttcct tcaaagacct tttgcttatc gatggcttgg tagagcccga
     6901 catggatgta ttttccaacc gctccagcga cggcatcctt ggttttcaaa ggacgttcca
     6961 aacctggact ggtaatttct aggaaatatt gttctgggaa gggatctggc ttgatggtgt
     7021 ctaggacagg actgatcatt tctgtcaagt ctgccgtgtc gttcaaggta attccttcgg
     7081 gtttatctac aaaaatactg agaatcatgt cactgccaat ctttccatac tcgatatcca
     7141 cgagttcaaa aggagcttct atgacaggtt ctacaacttc tctgactaat tctacgattg
     7201 ttgcgattgc gtccacctcc tcataagcaa gaggcgaaga tatttccccg cctcactttt
     7261 catattctta ctatcagtat agcacgtttg ccaacttatg taaagcaaaa gcacttgtac
     7321 agcctaattt taggctatct cttaaaattc tgcctcaact cggtagataa cttgcccctt
     7381 gtttgagaat ttttgctcgt attctgtcat gacattgcct tcaaaatcac tggcatgcaa
     7441 atctagccag acaccattga gtttcatgcc atattgagaa aagctcacta aactgtactc
     7501 aaacaagcca cggttatccg tcttgaaatg aatttctcca ttttcaggca agatacgttt
     7561 gaaggtatcc aaaaaggtct tgtaggtcaa acgacgcttt tcatggcgtt ttttcggcca
     7621 tggatctgaa aagttcagat acaagcgatc aatctcaccg tcttcaaagt agtcagtcaa
     7681 gtcagaacca tctacccaca agagcttgat gttagacact ccagcttcaa gcaccttgtc
     7741 caaagcgtag ctcaaaacag acttttgaat atcaatcccg atatagttga tgtcagggtt
     7801 ttgcttggcc atacctgaaa caaaggcacc ctttccactt ccaacttcca catgaatggg
     7861 attatcattg ccaaacaagt cccgccattt tgccttggct tccaagggat tgaggaccac
     7921 atactgggga tttgcctcta gtaattctgt tgccccttta cgatttctaa ctctcatctt
     7981 ctctttccat acttgtctcg gaaatgacgc aaaccatgaa tctcccgatt gacattttct
     8041 aaatcttggt tcatataata cttggaaatc tgactcaaat aagacaattg accataccaa
     8101 tacaatttac ttaataccgt ttgattgtac ttgtaaccgt agtaggtcaa ccattccttc
     8161 cactgatgtt ctgaaatata atggcagagc atatgggcca catcaaacat gcgatcggtc
     8221 aagcgaaccg aatcccaatc tactaaataa atcaagccac tatctgtctc aatccaatta
     8281 ctatgtcgta catctccatg gacaatggtc gcatggtcct ctctaaatcc tggaatagtc
     8341 tgacgtaaat cagccatcac ttcactgata aaatgatttt tacgcaaagc atctggagcc
     8401 gtttcctgcc aagactgtag taaatctaca ggtgtttcca tggcatagcc caaacgactc
     8461 aattgtgtca tcaacggacg tgagcgatga agacgggtta aaatattgac gatttgctta
     8521 cgattcatat catagggggt caatatcttg cctgtcaacc attcttgagc acacatatca
     8581 cgcccatctg ccaaacgacg actccataat aattgtggag caatttgttc tctagctaga
     8641 ccaggtagga ttggagaggt gttcattttt acaaagatgc gcttcccatc aggatagcta
     8701 cccatataag ccttgccact tttcccaggt atgggagtca gtgttagctc attatcaccc
     8761 aaatccattt ctttcctccg tataaagttt ccttactatt ctactagttt ttggcctatt
     8821 cgtcaaccac tctaacccga ttctcaaaga aaagcctctg gattggcttg ggtatcatta
     8881 tagcttgaaa aaggtaagca ccgtcttctg cttacctttt cataattttt ataaataagt
     8941 taagagtggc caactgttgt aaagcatatg gactagagta gaaacccata ggttttgact
     9001 ctttttataa gcaaagccca gcaacaaacc cccaagtaga taaaaaataa acgaagggac
     9061 attagaaggt ccatgcatga aagaaaagag agaggaagtt agcacaagcc ctaaccaaga
     9121 attgtcaaaa accgcacctt gaagaagtcc tctgaaaatg agttcctcct ggatggggcc
     9181 aaaaactgag gtagttaaaa tcaaagacag ggaaattcct ctgctgactt ccgccaagtg
     9241 ttggacccct gagccagaag aaacagcgaa gaaatgaata taaaccagcg tctcaagtac
     9301 acttagaaga aagattgcaa tgaaaagcaa aagttgtttc ttgcttaaca tcccaaaaga
     9361 aatcatttca aactttctag cataagcaac actcagcgag gttacaagaa gacttacaag
     9421 aatcaatgca gattcatttt gaaaaaaaac cccatggtta atcgtataag gaactgtaat
     9481 gacaattatt agtactaaaa atccaaaaca caaggcatga aatttatcaa aaaactccat
     9541 aaaattatcc tcctcaacaa acagacttcc tacacgtcat tctttagatc tagcctttcc
     9601 aaaacaaacc attttagcaa ccaaaatccg accacatagc cagcccctat gaatatagcc
     9661 attaaagcta gcatggaatt taggaaatta aaaaccaccg cagatacaaa ggttagcaca
     9721 aaaacattaa aagcaatggt gtcagaagcc aagactagaa tatagggtgt caaccgatct
     9781 aaagttttgg aatctaggaa aaataagtgt ttatacatga tgacctcctc tatggctgaa
     9841 aagcaagcct tttgtttttt taccccaaga ccctatgtag aaaagtgagc aaaaacggga
     9901 aggtcgctac aatattattg atcacatgca ccgcatagga tggataaatg ctcttggtat
     9961 agcgggtcaa accagcaaag atgattccaa ctgttgcaaa gacgaagata tctaacagac
    10021 taggcaggct tgaaaaatga gggagagcaa ataaaataga aggaagaagc aaatcaagac
    10081 caaatcgcga atgcttaaag aaagcatgtt gcagtaatcc tctataaatc aattcttcca
    10141 tcagtggaac cagaaagaac agggctatat aaatacctag ctctgcaaag ttagtcccac
    10201 tataaccaat caatacagcc caaccttccg cagttgactg aacatgttta gctgtctgaa
    10261 cgttaaaaga gatctggaac actaccacta atactgtcaa aatcgaatac caaagccatt
    10321 tttttcttgg aatgcggaag agataaccat ggcctgtctt aacaagaacc acaatcatga
    10381 ctccaataaa aagtaaactc aagatatttt gaatccagaa taaattgcct atctgagaag
    10441 aaaattgcca atagttttgg acgataagcg tcagctgaga aagaccaaat acgaaaaata
    10501 ggtaggataa aattgcactt gttttgaata gaagctgata ctttttcata gaaattcttc
    10561 ctcatttctt atatttcagc ctatattcca cataaatgaa aacctgctcc tatataacca
    10621 aggtcacgat aaccacgcgg gcagtaccca cttactggga aaggatctga attttttaat
    10681 atagaagggt aaggaccaaa tggactgtcc tgataagcat accctctata aataccagat
    10741 acatccccac cctctataca agcaagcatt tcagtatcca taatttcaaa ttgttccatc
    10801 atttttgtat ccatgacaaa tactcctttt tttattttta attttttgtc ctgttacaac
    10861 tttgacaagt ttagtatatc atcgtttatt aatttttttc atccaaatct tgaattgtca
    10921 tcgaaacgtc ttgaattgtt aaaaaattta aaaagcaagc attaaaaaca tacttgccct
    10981 ttcactcttg tctataggaa aattctagcc atatgttatt acaaacaaat aatatcattg
    11041 cacctgttta ccatctattt ttatttatga attctttgtc tccacttaat catacctgaa
    11101 gtaattaaga gtgcaaccgc tagacgtaat agattgtaac tttcttccac tcctgtatta
    11161 ggtagtaatt gtttactcaa atgactggtt ttggagggaa aatcattccc ctcaaccttg
    11221 gataaaggag tttgtaactt ctcagtattt tcagaaatcg tctgagatga ctcccctcgc
    11281 ttcttcttat ctatcattac caaatcttca gactgaacag ttatagtgtt agaaggagga
    11341 atattatcct tcaatggctc tctgttatca ctgttattag tcgaattttc ctttgaattc
    11401 ttcacctctc ctgttttatc atcacggaca ctttcagtta ctgtttcctc agaagatttc
    11461 actgtctccg tttgcttagg tgagtttctc gaatacatat aatcatacac ctgttgtgca
    11521 gttgctccgt caccaaccca tccagatgtc ggatgtccat ttcttaaata caaaattgtt
    11581 ggtgttcctg gaataccgac ttttttaaaa tagaaactct ctcgcttccc catcaaaatc
    11641 cttaccatcc aaatcataat actctaactt cttttcaatc aagttattaa attctttcaa
    11701 ttcaggagaa aattgacgac aatgagagca agtttttcta ccaaaataaa tagtatgttc
    11761 caattgatcc cctgtaaaag attgacgaac agcttcaata tctatctttt taaagtccgt
    11821 aacattcttc tcatactctt ctggggttac aacagaaggc ttatgctcca ccttttcctt
    11881 agccgatggc aaatctggcg ccacaagaga actattattc gcctgggtag atgaagtaat
    11941 aggctgttct tctgcatatg caagacctgc agtaatagat gcaaatacaa gtggtacaag
    12001 taatagcgat tttatctttt gtttcttcat aagaatactc ctttacattt gttatcttta
    12061 tcttacctta tttaagatta caaattcatc taaatcttaa atggtcatcg aaacatcttg
    12121 aattgcaaaa acttcattat aaaagggtaa gtatttcaaa aacacttacc cctttatact
    12181 ttattaagct ctacttttta taatacttca agccccaaat gagaaaaagt ataataatca
    12241 agcagagaat agcaccaatg taggcaatta tttgttggta ggattctcct gctgtgatcc
    12301 ctctatacaa acaaataata gacataaaac ctgtcaagcc gatgaacatg atttgattag
    12361 ttctaggact aaccaaatca tcgtcttcaa actcttttat ccgcatttcc ctagtgaggt
    12421 aaacagtgac caaaatagaa gctaagttaa taaccactaa aagaaattgg aaaactgacg
    12481 aaatagaaat agataagtag aaacaagcaa aggcaactga cccaggaaca atctcacaaa
    12541 gaatatgatt ctttttaatc aagaaaatct ttcatttctt ttctttcttt ctaattaaag
    12601 aaaaatagat cataaccgca atcatatagg ctatggtata aaatagatta taccaaatac
    12661 tctccctaag cggataaaga aagatggaca tgatcagata cagaacgaaa ataatcagta
    12721 tttttttctt cataagattt cctcctaaat gtgcgattta tcttagttga gcaagaacat
    12781 ttacactgct agtatagcac ttattttgac cttggatcac tcaaatcata aatggtcatc
    12841 aaaacctctt gaattgtaaa aattaaaaaa agcaagcatg aaaaacatac tttcctcttt
    12901 atattgtatt gataccaact tgtttgtaga cttttcatcc tgctatcaca tatcattttg
    12961 acaggcgaaa caatattcaa gaaactcccc tgtaaattaa gctagcaaat acaggggaga
    13021 aatttatttt ttagggggta ctatccgtat cctttttgga agattttgaa aatatttttc
    13081 taattaagtc atccatataa ggaccaaata taccaactac taaaccaata ataaaacttt
    13141 taaaatccat aattaccacc aacatgttgc tacataggct acacctccaa gtatagctcc
    13201 acctgcagca ccagttacac ctattcctat agcaaatggt cccaatagaa atgtcaaacc
    13261 gttgttgcac acccatcaat tgcgccatat gcaacccctg ctgcacaact aatttttctt
    13321 ccccaatcaa tatctccacc ttcaacgcaa gcaagcattt cattatccat aacagaaaat
    13381 tgtgacatca tttttgtatt catatagtat gtcacttttc agttacggaa caagtttaat
    13441 ataaaaatta tcaaaaaaac ataggcaata aagagaaaaa ttaatttatc atagattaga
    13501 aataatatga caaaacaatt caatgatgtt aattcaatag tcttttgttt tttatcagag
    13561 atacttatgg atagataaat aagataggtt tgaaaagcga agagaataat aaagaatata
    13621 gccttcataa aatttagctt tcatttttat gatgtagcgg tataggctaa atatccacaa
    13681 accactgctc ctccaattcc tcctattgca gcgccccatg gtcctagaat tttcccatat
    13741 ttcactccac ccgcagcacc agttgctgca ccttgccatg ttcctgtttt aatgcctagt
    13801 tgaagacctc ttgctgctgc tcctccaaca cctgctttgg caaaatctcc ccaattgcat
    13861 ccgccacctt caacgcaagc aagcatttca gtatccataa cagaaaattg tgacatcatt
    13921 tttgtaccca tgacaaatac tcctttttta aaaaactaaa ataaatcaga atagaatcct
    13981 cataatttta ctataagtct taccaactta gtcccaattt atcaccaacc atacctccta
    14041 agcatgttaa tccatcccca attgcaccaa tgtgtgctcc aacaaatgca ccaacaagtc
    14101 cagctactcc taaagtggcc aaacctgctc cagttccacc agttataatt cccgtagtga
    14161 ctcctgtaat cagtgcattt tgacaatcag tggagctata ccccccttca actttcgcaa
    14221 gcatttcagt atccataacc tctaactgtg acaacatttt tgtattcatg atgaatacct
    14281 cctttttatt ttcaatttgt taccaaagtc ttaaattcaa taaacaaata gattttttat
    14341 agtatctttt tgattttctt aaaaaagtat atacgtctac tatcttctta aaggtagcag
    14401 tacctatttt ttagtctaag atttcaataa tcttgagtat ctaaaatatc ttaatttcgt
    14461 tattctcctt gcaataaaaa gttttactat actatttatt aacttgcaga aagcaaaaaa
    14521 tattagtaaa taatagttta tagttaagtt ttttattcct accaatccat caactaagta
    14581 aagcatcaac gattacataa acgattgata atataattaa aattttacta actatcttat
    14641 tctcatcatt cttagataac tttgatattt tgtaagtaag taaataagac agtaaattaa
    14701 tagcgataat aatactatat ttaagaatca taatcttaca aagaggacat aattcctgaa
    14761 cctacacaaa taagtgttgc tgctccccca gttatcggac cagtcgcagc agctaatagt
    14821 actgctccaa tacaaccacc gattgcagat cctaaattgc ctcttcctcc actaactatt
    14881 tcgagttctt cattatccat aacagaaaat tgttccatca tttttgtatt catgacaaat
    14941 actccttttt tattttttat ttttgtcttg ttgtaacttt gataagttta gtatatcatc
    15001 gttttttaaa atttttcatc cagatcttga attgtcatcg aaacgtcttg aattagcttt
    15061 tttatttcaa gccacctcta aatgtttaaa aaaaataatt tctaatcact tttttaccat
    15121 tcaggaagtt ttaatgacta ttcaagattt cataaaatat gaacttagtt ttatgacata
    15181 atagacctat ccactatatg aaaggaattg ccaatgactt cttataaacg tacatttgtt
    15241 cctcaaatag atgcgagaga ctgtggtgtc gctgccttag cctcgattgc taaattctat
    15301 ggttcagatt tttctctagc tcacttgaga gaacttgcaa agaccaataa agaagggacg
    15361 actgctcttg gcattgtaaa agccgctgat gaaatgggct ttgaaacaag acctgttcaa
    15421 gcagataaaa cgctctttga catgagtgat gtcccctatc catttatcgt tcacgttaac
    15481 aaagaaggaa aactccaaca ttactatgtt gtctatcaaa caaagaaaga ctatctgatt
    15541 attggtgatc ctgacccttc tgtaaaaatc actaaaatgt caaaagaacg ctttttctat
    15601 gaatggactg gagtagctat ttttctagct accaaaccca gctatcaacc ccataaagat
    15661 aaaaagaatg gtctactaag caagcttcct tcctctgatt ttcaaacaaa aatctctcat
    15721 tgcttacatt gttctctcaa gcttattggt cactattatc aatataggtg gttcttacta
    15781 tctccaagga atcttggatg aatacattcc aaatcagatg aaatcaactt taggaatcat
    15841 ctcagttggt ctggttatca cctatatcct ccaacaagtc atgagcttct ccagagatta
    15901 tctcctaacc gttctgagtc aaagattaag tattgatgtg attttatcct atattcgcca
    15961 tatttttgaa cttcccatgt ctttctttgc gacacgtcgt acaggagaaa tcatttcacg
    16021 gttcacagat gctaactcta ttatagatgc cttggcttct accattcttt ctctttttct
    16081 ggatgtttct attctgattc ttgtaggggg agtcttgctg gcacaaaacc ctaatctctt
    16141 ccttctttct cttatttcca ttcctatata catgttcatc atcttttctt ttatgaaacc
    16201 tttcgaaaaa atgaaccatg atgtcatgca aagtaattct atggttagct ctgccattat
    16261 cgaagatatc aacgggattg aaactataaa gtcgctcacg agtgaagaaa atcgctatca
    16321 aaatatagac agcgaatttg tagattattt ggaaaaatcc tttaagctca gtaaatattc
    16381 tattttacaa acgagtttaa agcagggaac aaaattagtt ctgaatatcc ttatcctatg
    16441 gtttggcgct caattagtca tgtcaagtaa aatttctatc ggtcagctga ttacctttaa
    16501 cacacttttt tcttacttta caactcctat ggaaaatatt atcaacctcc aaaccaaact
    16561 ccaatctgcg aaggtcgcta ataaccgttt gaacgaagtc tatctagtcg aatctgaatt
    16621 tcaagttcaa gaaaaccctg ttcattcaca ttttttgatg ggcgatattg aatttgatga
    16681 cctttcttat aagtatggtt ttggacgaga taccttaaca gatattaatc tcacgattaa
    16741 acaaggagat aaggttagcc tagttggagt tagtggttct ggtaaaacaa ctttagccaa
    16801 aatgattgtc aatttctttg aaccctacaa agggcatatt ttcatcaatc atcaggatat
    16861 taaaaacatt gataaaaaag tcttgcgccg tcatattaat tacctaccac aacaagccta
    16921 tatctttaat ggctctattt tggaaaactt aaccttgggc ggtaatcata tgattagcca
    16981 agaagatatt ctaaaagctt gtgaattagc tgaaattcgt caagacattg aaagaatgcc
    17041 tatgggctat caaactcagc tctctgatgg agctggtcta tcaggaggac aaaagcaacg
    17101 aatcgctctc gctcgtgctc ttttaactaa agctcctgtt ttaatactag atgaagctac
    17161 tagcggtctt gatgtcttga ctgagaaaaa ggttatagat aatcttatat ccctaactga
    17221 taaaaccatt ctctttgtag cccatcgtct cagtatagcc gaacaaacta accgtgtcat
    17281 tgttcttgac caggggaaaa tcattgaagt tggtagtcac caagagttaa tgcaggcgca
    17341 aggcttctac catcatctat tcaataaata aggagaatgt catgaatcct aatcttttta
    17401 gaagcgtcga gttttatcag agacgttacc ataactatgc gacagtgtta attatacctc
    17461 tttcattact gtttactttc atcttgattt tctcccttgt tgccacaaaa gaaattactg
    17521 ttacttccca aggagaaatc gcccctacaa gtgtcattgc atctattcag tcgaccagtg
    17581 ataatcctat ccttgctaat catttagtgg caaatcaagt agttgagaaa ggtgacttac
    17641 tcatcaaata ctctgaaaca atggaagaaa gtcagaaaac tgccttagca actcaattac
    17701 aaagacttga gaagcaaaaa gaaggacttg gaattttgaa acaaagctta gaaaaagcga
    17761 ctgatctttt ttctggcgag gatgaatttg gctaccataa tacctttatg aattttacta
    17821 aacaatccca tgatattgaa ctgggtatca caaagactaa cactgaggtt tcaaatcaag
    17881 ctaatcttgc caatagcagt tcatcagcta ttgaacaaga aattacaaaa gttcaacaac
    17941 aaattggaga atatcaagag ctgagagatg ctatcataaa taacagagca cgcttaccaa
    18001 ctagcaatcc acaccagtca attttgaatc gttatcttgt agcctcacaa ggacaaacac
    18061 aaggaacggc agaggagcca tttttatctc aaattaatca aagtattgca ggtcttgaat
    18121 catctatcgc aagcctcaaa attcagcaag ctggtatcgg aagtgtagca acttatgata
    18181 acagtttagc aaccaaaatt gaagtactcc gcactcagtt tttacagaca gcctcacagc
    18241 aacaactaac tgtggagaat caattaacag aattaaaagt acaactagat caagccacac
    18301 agcgtttgga aaacaatacc ttaacctccc caagtaaagg tatcgttcat ctgaacagcg
    18361 aatttgaagg taaaaataga attccaactg gtacagaaat tgctcaaata ttccctgtca
    18421 tcacagatac aagagaagta ctaatcactt actacgtatc ttctgactat ctacctctac
    18481 tagataaagg acaaactgta agattaaaac tggagaagat tggaaatcac ggcaccacca
    18541 tcatcggcca acttcagaca attgatcaaa ctcctaccag aacagagcaa ggaaatctct
    18601 ttaaattaac cgctcttgca aaactatcta acgaggatag taaactcatc caatatggct
    18661 tacaaggtcg cgtcactagt gtaactacaa agaaaacata ttttgattat ttcaaagata
    18721 aaattttaac acattctgat taattttcag ataacactct ataactattt attatcttat
    18781 caaaaaggag aatcataaca tggataagaa acaaaaccta acttcatttc aagaactaac
    18841 aactaccgaa ctcaaccaaa ttacaggtgg aggattgtgg gaagatttat tatataacat
    18901 taatagatat gctcattaca tcacataaga acttcatcat ccaatacaac tataaaaaaa
    18961 taagaccgag aaacaagtac tctcggtctt atttttcatc attctgtagg tatcatagta
    19021 agcacctgac gaaagacttg atcttgacag gtagtattta aactggcatt gggataactt
    19081 tccacaatct tcataacggt atagagaccg acacctctct cctccccttt agaactaact
    19141 ccaaaggaga agatttcaga aatatctatg ccctcttctt tgatggaatt ttcgatgatg
    19201 aaggtctcct gtgctccact ttttaaaaag gcaattgaaa catgaggttg accggcctct
    19261 gcactcgctt caatagcatt atcacaaagg atagacacaa tggttagaaa atcaagcagt
    19321 ctcatcccct caacctgaat ctcttcagga acttcgacat taaagacaat gtccttttct
    19381 ctagctttta taaattttcc agctaggaga cttttgaggg cacgatcccg aacattcacc
    19441 aatctgccca ggtcatattt attgttctgc aatttctgac tggaatcctt taagacggag
    19501 ccatagacct cttttatctg ctccatatcc tcctcttcaa tgcccagacg taagctagtc
    19561 aagaggttgg tataatcatg acgaaagctc cgtacttcct tgtaaagctc ctctatatgc
    19621 cgactatagc gttccatatc tctatagcgc agggcctgct cttgttccaa tctctcatag
    19681 agtttttcct tcaaataggt atccaatttc ttgataaccc ccataaaaaa gagtaggtaa
    19741 aacactagga tgagatggcg aacagtcgtt gattgaatac cttgctcata ttcaaagtaa
    19801 gacagacttt gtatcaccaa atagtaagcc cccattatcc agttaatctt agtcagggac
    19861 ttttgaaaac ctgtatctag aatctccctt ctcaaactag taaaatcata gtccaaccat
    19921 ttcaaaaagg ctagagaaat gaagaaattg aaaattatta tacataaccc agtaaatgag
    19981 tagccatcat atacttgctc ttgtcccaaa aatggaagca caaaatagga gactcctcta
    20041 taaaagagat tcaccaatat cattggaaag agaccataaa agaaaaggag ttttttagga
    20101 agccctctta acaataagaa agacaagcct atgccgtaca agggttccat aaaataagat
    20161 aggtaaacat ttcctaccat atagccaatc attacaaaaa caaaggccaa cagtatcttc
    20221 aaaagaaagg ccttaaaaat cctctcaaaa gtgagaccaa ttccatctac cttaaagaaa
    20281 atgacaattt ctagcccatt agtaataagt gtatacaata taatccaaaa aatatacata
    20341 aatttcctag ttcagtgtaa gttattaata gcctcagaca cttccctgac cttataacgc
    20401 gcgatcagac agcttccacc attgggaaag aaaaggagtt tttctttctt atccaaatgc
    20461 accacatttg caggattgat gagaaaagag cgatggcact gcaagagacg gggttcctgc
    20521 ttaaaaacct cctctaaact cgccgtaaat tctagcctgt ccgtcttggt atagagaata
    20581 acacgatggg gtcttgggga tgtttcgaga tagtaaacct ctttgaaagg atattggaat
    20641 tgggcaaatt ttgatttaaa gtaaaagcaa tcttccgcca gacttttact atcttgacta
    20701 ttggcataga ggagggctgt ctcgatacga gattcaaact cctctgccga aagggcctta
    20761 tcaatgtagt ccaaagctga cacttggtag cgaaaggaca gaggcataaa ctccgagtga
    20821 gtcgtcacaa agacgattag ggcataaggg tcccgatccc gaatctttct agccacttcc
    20881 agccccttca tctcctcatt tcgaatctca atgtccaaaa agaatagctg atgggccccc
    20941 ttctcatgca cctctgccag cagttggtcc ggcttgccaa atacctcaaa agagctagga
    21001 atgatatgat gtgctttcaa aagtttctca atcgtcgttt caattctagt ctgttgggaa
    21061 aaatcatctt ctaaaacaaa tattctcatc ttcttactct ccctctttca accatttttg
    21121 acgaatttct ctatagcgac gtctggcata cttgacagcg tattgacctt cttctccgag
    21181 aaaaaggatt ctttcttgaa aatcaatcga ttttaattta tcaagattaa ccaaacaatt
    21241 tcgatgacat ctcatcaagg tttccccata ttggttctca agattactta aattttgaag
    21301 catattaaaa ctagcttctt ctgtaacaat ctgtacggta tgggctttag ttggatgtgt
    21361 ttggatatag tagatatcgt cgacgtttac tttatagact gttttctgcg tctgaataat
    21421 catatgtttc attggattct cctcctaaga aaagaataac agaaaaggtc tagcccaagc
    21481 ccacccaatc ctaaacagtc tttgaaagct cctaaaagga ttatttttct acaacatcat
    21541 gatgagctta caactatttt atcataaaat tcactcagcg caattcaaga catttcaatg
    21601 acaattaaag atttgaataa aaaaaaagaa ttaaaagtga taaactaaca gtatcaaaac
    21661 acagttgcca gaagtaagac tggcacccag attaaaggag gcattcccat gacagataca
    21721 gaccccatca aaagagctca tactttgatt actgacttaa acaaagccta ccaagcatgc
    21781 aaacaggcaa gcgctgacga cgtccgcttt caggagcaat taaactctat tcttggtttt
    21841 ctagccaaga ctgaaacagt ggataatcga ttcttgattg aactagaaaa attttaccag
    21901 acttccagtc ttctcatggg actcagcgct cttgatccag atgctccaac tcgcgccgct
    21961 tggcgggcct atgaccgctt ccactttgac caagtcaaga ccaagttaat actcaatgag
    22021 aatcaaagag caaactagga agctagccgc aagctgtact tgagtacggt aaggcgacgc
    22081 tgacgtggtt tgaagagatt ttcgaagagt ataagtctct acggaccaac cattatcctg
    22141 tagtaacaaa aaagaagctg agacaaactg aactcaactt cttttttagt gtcgtatcag
    22201 caatgttagt cctgcatctg acgttttacc tgataaggca aatacaagac tagtaaaacc
    22261 aaaccagctc ctagtaaggc tagaagggct agtttttctt ggaaggtaat caacccaaca
    22321 actaattcca caagtaaaac aaaactggtc aatcctcgaa ctacctcctg taagcctttt
    22381 tccttttgcc ccttgtccag cggaaagagt tgggtcaaat actggtagtc aaaggcatga
    22441 tagagggcca gcaactggaa gagcaagagg tagttaaaga gaactaccac tgctgtcgca
    22501 atccaagctt gctcgataaa aacctgcgcc agtacggaaa gtaagagaag acgaagactg
    22561 agagcaaaga ggtcgccatt tcgcagataa gaacgcagat agagattttg ccaaatcttc
    22621 ccaggcacct tctgaacagc ctttaagata aagtccagat aggcacgacg cttgacactg
    22681 tttgaaattc ccttgacctg cgtaaagagg gcaaagaaac gaagcaagac ttgcttacgc
    22741 ttgctttctt gagaaataag atagtcccag tccagtccag tttcagtgaa aaatttgctg
    22801 gccttttgac gaaagtggaa atattttcct acccccaata aaagcacata gagcagaaaa
    22861 actggcaagc cataacccat tgctaaaaat aaaggcgcaa ataacagcaa gaaaagggtc
    22921 tgtacaaaga gccaaaagac tagggaaatg ccagtttgac gcttgagatg gagcttgatt
    22981 tcctcttctc caactaagag aaagagcttg tctggagcct ccatataggt ggcaattcct
    23041 ccccaaagta agagtaaaac agacgtaatt cctacaaaca aaaggatagg ccaatgattt
    23101 tcaggaaaat gttgtaagag ttgactgtac tggtaggcta gaaagcccaa caggacaagc
    23161 aggaacaaga caaagtggtc attgagcaca tagcgcagat aaccaagaca ctccttacga
    23221 aaggcctgct ttctctttaa aaacaagtct ttcatagatc ctcctctttg gtcagagcca
    23281 agtaaatatc attcaaacta gcctcaggca tatcaaaggc ttcacgtagt tgcaggagat
    23341 tgcctttggc acgcacctct cccttgtgaa gaatgacaaa ggcatcacac atcttctccg
    23401 ccgaatccag cacgtgggta ctcatgagaa tagacttgcc cttttgcttc tccacttcca
    23461 aaagctgaat caaatcagaa atagccagcg gatcaagacc aaggaaaggc tcatccacga
    23521 tgaaaagact tggatccacc acaaaagcac agataatcat gaccttctgc ttcatccctt
    23581 ttgaaaaatg aacagggaac cagtctaatt tctggtccag acggaacatc tttaacaagg
    23641 gctctactcg ttcgaaagcc actttttgct caataccgta agccatagca accgtttcga
    23701 tatgctctct gagggtcaat tcctcataca gactaggcgt ctcaggaatg tagccaatct
    23761 gcttgcggta gctagtcgca tctccttgca gagtcaggcc attgatattg atggagccac
    23821 tataaggtgc caacagaccg ataatctcat tgatcgtcgt tgatttccca gcaccattga
    23881 gaccaatcaa accgaccaac tgcccacttt caacagtaaa ggacacatct ttcaaaacag
    23941 gaacatgaac atagccacct gtcaggtttt taatttctaa catattttct ccaaatctgg
    24001 tataatgtag ctatattata tcaaaattca gtacagtaga ggtagatttt atgtcagatt
    24061 gcattttttg taaaatcatc gcaggggaaa ttcctgcttc aaaagtatat gaagatgagc
    24121 aggtccttgc ctttcttgat atctctcaag taacactagg acacaccttg gtcgtgccaa
    24181 aagaacacta tcgcaatctt ttggagatgg atgctacgag cgccagccaa ctctttgccc
    24241 aagtaccaaa agtagctcaa aaagtcatga aagtcactaa ggctgctggt atgaatatca
    24301 tttccaactg tgaagaagtc gctggtcaaa cagtttttca tactcacgtt caccttgtgc
    24361 ctcgctacag tgctgacgat gacctcaaga ttgattttat cgcccacgaa ccagactttg
    24421 acaaacttgc tcaagtcgct gaaaccatca aaaacgctta aggagttgct atgaaattat
    24481 ccaacctact gctatttgca ggagctgcag ccggaagtta tctggttaca aaaaatcgcc
    24541 aaaccatcac agatgaagtc ttgaatacca ctgaccgcgt tcaagctatc aaggacgatg
    24601 tggatattat ccaaaacagc ctgcaaatca ttaaccagca aaaagaactt atcaaggaat
    24661 accaagaaga cttgacttac aagtttaagg tcttggaaaa ggatatccag actagactag
    24721 ctgtgataaa agaaatgcag ggaactgaag ataagtaaaa agagcccccc ggctcttttt
    24781 taaaacgtaa acaagttata gtgaattgaa tttagaatag cacatcatag tttctaaagc
    24841 attgctagag attactttaa aatcctctta tcaatttgtt catattctat ttcaatctac
    24901 tatatatatc ccatcaacta tgcatcataa tttaggtaac tcatactcaa taaaaatcaa
    24961 aaagcaaact agaaagctag gcacagactg ctcaaaacac cgttttaagg ttgtggatag
    25021 aactgacgaa gtcagctcaa aacactgttt tgaggttgta gatacgactg acgaagtcag
    25081 ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagtaacc atacccatga
    25141 caagccgacg ctgacgtggt ttgaagaaat ttttgaagag tatcattcac catttcacaa
    25201 ctaaatcgaa gactttctgc cgtaatgaaa acacctgaaa cagcttggtt tcagctgtcc
    25261 ggaaacttta agacttaggt tcaaagttta ggtatggaac ttcgaagaag gtcgctaccg
    25321 tccgtcatta cttaggaaag tcttaaaaaa tctataaagt aaaaagagcc cgacagctct
    25381 ttttgtttat actcaatgaa aatcaaaaag caaactagga aactagccac gggctgctca
    25441 aaacactgtt ttgaggttgt agatacgact cacatatata cggcaaggca acgctgacgt
    25501 ggtttgaaga gattttcgaa gagtattatt ctccatcaaa ggcatcttta atatggtcaa
    25561 agaagccttt tttctttgga tttactttca agccaccagc agccgcgaat tctttcaagg
    25621 ctactttttg ccggtcgttc aagcctgtcg gtgttacgac attaacagta acgtattggt
    25681 caccaactgc accgccacga aggctcggtg cccccttact acgtaggcgg aatttcttac
    25741 cagtctgagt tccctctgga ataaccaatt caacatcacc gtgaacagtt ggaatatcta
    25801 ctgtatcacc aagagccgct tggacaaagt tgaggttgag attgtagaag atagtcgttc
    25861 cttcacgttc aaacttgtcg ctagcttcca cagaaactac tacatacaag tcaccataag
    25921 gtccaccgtt aaagcctgct tcaccttgac cagcgaggcg aatttgttga cctgtttcca
    25981 caccagcagg gattttcaca tgtacgctat gagcttgttt ctcatgacct gttccatgac
    26041 aggttgtaca tggatatttg atttcttttc ctcgaccgtg acagacatca caggttactt
    26101 ggcgacgcat cataccaaga ggagtctgcg tatcgacgtt aatgacacca gcgccatgac
    26161 agcgtccaca agtgactgga cttgtccctg gcttagcacc agagccatta catgtacgac
    26221 agccagcttc acgatgatac ttaacttcct tctcagttcc gaagatagct tcttcaaagg
    26281 tcaaattgac acgatactgg agatcatctc cttggcgagg agcgtttgga ttgcgcgaag
    26341 aaccgcctcc gccgaagaaa cttgagaaaa tatcctcaaa accaccgaaa ccacctgccc
    26401 cattgaaacc gccgaaacca ccagctccac caaaaccacc attggcgcct gcagcaccat
    26461 actggtcata ggcagcacgt ttttggtcgt cactcaaagt ctcataggct tcttgaactt
    26521 ccttgtactt gtcctcagca ccaggctcct tgttgatatc tgggtgatat tttttggaaa
    26581 gcttacgata agcctttttg atttcgtctg ccgaagcgtt tttggatacc cccagacgat
    26641 cataaaattc agtattgttc ataattcaag atacaaaggg cgtcaaacgg acacagccat
    26701 ctaggagttt tgacgacgga cgcttacgtc ctagaaaaaa ctatcttttg ggcacagtcc
    26761 gtagcccgtg ttcagttacg aacaccctta ttcctttcta ttgatgttct gaatcgaacc
    26821 accatttagg tcgggttcaa ttcctttctt ttatatttag taagaaattc tgaaacccgt
    26881 gttcagttac gaacactctt tgttcccttc tattgatgag acaaaaccca aacaaatgag
    26941 attccgtccc tttttatcga aaaacagagg ctgggttgca acctctgcaa ataagtaaat
    27001 taactttatt ttgattttaa atatcctata agtacagaaa tatttatata tttttgtctc
    27061 ttcttagtat ttgcctatat aattccaatt gatcataaga aatacaacta ctgtccaaat
    27121 cataccaatt atgaacggta ttacaaattt atatgtttgt atttctaatg atatcatagc
    27181 tgataccagc gctgatacaa atgcagacaa caatactgag gtaacaaaat tccttaccgc
    27241 ttcatcattt ttgatacaac tatcatgttc agctaatata atttctctat atctagctaa
    27301 acattctatt ggaatattat caataaaatc ttttgatgtt ctatagttat cagcttgatt
    27361 acaaaaatca ttataaactt ttcgtgtatt ttttagatac tcttcagcca atacactcat
    27421 cttacttttc cgtaaactct ccgtctacga cgtcattgcc tgcgtttcct gttgcttgtg
    27481 cgccttctgc tccttcttga gcttgttgcg ctgctgcggc ttgttcgtag agtttaacag
    27541 caagtccttg agctttttcg ttcaatgctt caagttttgt tttcatgtcg tccaagttgt
    27601 tgtcttcttg agctttctta aggtcatcaa gggcagcttg ggcagcgtca cgttctgcgt
    27661 cgaagccttt accttcagtt tccttgattg tcttttcagt cgcaaagatt gcttggtcta
    27721 cttcattacg aaggtctact tcttctttac gtttcttatc ggcttcagcg tttgcttctg
    27781 catctttcat catgcggtcg atttcttcgt cagtcaaacc tgagttcgat tggatgacaa
    27841 tagtttgttc tttttgagtt ccaaggtctt tggccttaac agacacgata ccgttcttgt
    27901 cgatgtcaaa tgttacttcg atttgaggaa ttccacgagg tgcagctggg atatcagtca
    27961 attggaagcg tccaagagtc ttgttatctg ctgccattgg gcgttcacct tgaagaacgt
    28021 ggatatcaac ggctggttgg ttgtctgctg ctgttgagaa gacttgtgat ttagatgttg
    28081 ggattgtagt gttgcgatcg ataagttttg taaatactcc acccattgtt tcgataccaa
    28141 gtgacaatgg cgttacatca agaaggacaa cgtccttgac atcaccagta atcacaccac
    28201 cttggatagc cgcacccata gcaactactt catcagggtt tactgatttg tttggttctt
    28261 taccagtttc agctttaaca gcttcaacaa cggcagggat acgagttgaa ccaccaacaa
    28321 ggataacttc gtcgatttct gacaagctca aacctgcatc tgaaagggct tgacgaactg
    28381 gaacttttgt acgttcaaca aggtcacgag tcaaatcgtc aaatttcgca cgagtcaaag
    28441 tcatttccaa gtgaagaggt ccagcctcac ctgcagtgat aaatggcaag ctgatttgtg
    28501 ttgaagttac accagaaagg tctttcttcg ctttttcagc cgcatctttc aaacgttgca
    28561 ttgccatctt gtcagtagac aagtcgatac cgttttcttt cttgaattct gctaccaagt
    28621 ggtcaatgat tttttggtca aagtcgtcac caccaagttt gttgtcccct gcagttgaca
    28681 atacgtcgaa gacaccgtca cccaattcaa ggatagagac gtcgaatgta ccaccaccaa
    28741 ggtcaaatac caagattttt tcttctttgt cagtcttgtc caaaccataa gcaagagctg
    28801 ctgcagttgg ttcgttaaca atacgttcta cttcaagacc agcaatttta ccagcgtctt
    28861 ttgttgcttg acgttgagcg tcgttgaagt aagccggaac tgtgataaca gctttggtta
    28921 ctttctcacc aaggtagtct tcagcgtagc ctttcaagta ttgaaggatc atagctgaga
    28981 tttcttgtgg agtgtattct tttccatttg cagaaacttt ttcagaagtt cccatcttag
    29041 atttgataga gataactgta tctgggtttg tgactgcttg acgttttgca gcatcaccaa
    29101 cgatgatttc tccgtttttg aatgagacta cagatggagt tgtgcggttt ccttctgggt
    29161 ttgcgatgat tttgctttca gttccttcaa gaactgcaac tgctgagttt gttgtaccta
    29221 agtcaatacc gataatttta gacatgtgtt tttctccttg ttagttaatt ttctattttt
    29281 ctttttgata ctcttcttaa gtggcgacgc cgtcagagct tgacttcgtc aatctctttg
    29341 actaaacttt gagcctaagg tctcaaaatt tgcgcaaata gcgccacggc gaagagtatc
    29401 gtgtttggtt cgcttcgctc actattacaa agccaaacat attttatctt tctttatagt
    29461 ttattgtcgt ttcggacaag ttttctattc tgtaaattgc gacacgagga gtcgaaatcg
    29521 attttatttc gacgacgagt tagtaaggaa gctaggcaaa cgccatagcg attgccgttt
    29581 tctgacgagt tgctttagct accgtcagaa ttgccgaatc gaacacgccc taagctctga
    29641 gtaaaaaaga tagattttct tgtgttcatc gaacacttcg tcaatctcct atttttactg
    29701 cgagcttttt acgggctttg tatcttagtt atacaccact accattgctg ggcgtaggat
    29761 gcggtcatgg agtttgtagc ctttttgaaa gacttgagcg atggtatcta ctgggtgttc
    29821 atcgtctgct gggagagttt ggatggccat atggtagtta tggtcaaatt cgccatctgc
    29881 tgcgatttct tcaattcctt cttctttcaa agcgtgaatc aagctttctt gcaccatccc
    29941 caagcccttc ttcacatcat ctgtcaaacc ttcaactgca agtgcacgct caaggttgtc
    30001 aagagatggt aagattgctt ttgccaagtc ctggctacga taacgttgca agttttgacg
    30061 ttcttcattg gcacggcgtt ggatattttg catttctgca tgagcgcgaa gatatttgtt
    30121 ttcgaactca tctgcacgtt catttgccaa gtccaactca gacttttcag gagttgtttc
    30181 ttcagctgtt tccacaactt cctcttcttg aacttcttct acttcttcat tttttatatc
    30241 ttgggccatg ttcgcctcct ttaatgattt caatcttaat gtacttcgta atgattactg
    30301 ctgaggtagc ggtaaaaatc tgtcaacttc atggtcaaaa cacggttgac cacattgact
    30361 tgattgatta gctgttggta atccagatta actggaccga taatggctag aattccaact
    30421 ccccgataag gaatgaggaa cttactacta atcaccgcta ggtcagctaa acaggactct
    30481 tgaccgtctg caacacgaac attttgcatc tgatcctcac gcaacccctc acgaatctcc
    30541 aagaccacct tttgcggttg gtcaaagaac tgataggctt ctagattggc aaaattcaag
    30601 agattgacct tgcccgccat cacaatgttt tcgttgaaca tttccttaaa gatgtgttca
    30661 aagagatcga tgacattatc cgttgttgta aagtaacgct ggataatctg cggaatctcc
    30721 gtccgaatct tgtagtgaat atctaaaacg gtgtgaccga ggaaacgttc ctgaatgatg
    30781 ctcttcagtt tcagcaaatc ctcctgcaag aagttccttg gaatcagaaa ctgactagta
    30841 accgttcgcg actcgtctag ggtaaatacc gccaaggctg tatgttgccc caaaacaacg
    30901 atatcaaagg ctgtcaaacg ttgcctgctc ggctcaacat ccagtgccac taccgtacag
    30961 ccactcaggt ctgttagcaa gttagcagcc tcttgcagaa tatcctccaa tttgaagaat
    31021 tcctgatcaa aggctttgac aatctcatat acctcatttt cagccagccg gtgaaaatcc
    31081 agtgagtgtt tcacatagta ctgaaaacca gcaacacttg gcatccgacc gcttgaagta
    31141 tgagccttct caagcaaccc ttgtttttct agttccgcca tgtcattacg aatggttgca
    31201 ctgctagagt taatagactc ttgcaaggct tttgatccga caggttcgtg cgttttggta
    31261 aagatgtcaa taatcagatt taaaatatcc tgctgacgct ctgtaaccat ctcatcactc
    31321 ctctctgtct gatcaattag cactcaatgc atccaagtgc taacttatga ttctattata
    31381 cacccttaga agagaatgtc aagacaaaaa ctcaaaaaat tagcactctt ttatcaagag
    31441 tgctaaaaat caatctgaag acctagaacc ttgctattca tctactcggt atttcctttt
    31501 tagtctgaaa taaccatgaa ttagataaga aatcagaaaa gcatataaaa caaaaataag
    31561 aaagaaagac atagaaagat cctctctaaa caaactcgta caaacaacaa gaccacctaa
    31621 gaaagtagtt gtacaggaac gaagtctaga tcgcataact tcccatttga gccccttact
    31681 catatagact tgatcctctg gaagtaaatc agaagacgat ttacgaagaa tcgaacaaga
    31741 acctgctcct atcaattccc aacctctatc tctaaaatct tgcagttcat gcttatattt
    31801 tttaagaaat ctagaatcat agatacggta gatgacatcg tctggttgac attggtcaaa
    31861 atagaacaaa ccaaaacgac tcgttctata cctccaacct ttcaaatgca tctcatgtaa
    31921 atattcttct tccttgtcca aatcaacaat agtgaaaatc cgaaattcta ctctgctatt
    31981 cattgtctta ccccaaaatt agaaaacatg cctggcgtta tttattagat aattctttcc
    32041 gcttttgact caatctccaa aaaatataa
//
LOCUS       DAVDWM010000020        31631 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVDWM010000020 DAVDWM010000000
VERSION     DAVDWM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.020"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1..999
                     /locus_tag="VUW49_001129"
                     /pseudo
     CDS             1..999
                     /locus_tag="VUW49_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1127..2149)
                     /gene="cbpF"
                     /locus_tag="VUW49_001130"
     CDS             complement(1127..2149)
                     /gene="cbpF"
                     /locus_tag="VUW49_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HET7981056.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYISAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQYLGNRWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQESSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAINTTVGGYYLNY
                     NGEWVK"
     gene            complement(2168..2674)
                     /locus_tag="VUW49_001131"
     CDS             complement(2168..2674)
                     /locus_tag="VUW49_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET7981057.1"
                     /translation="MTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGS
                     TVYDASNRVVGVHTLGDGSNQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHY
                     RQHDKQTGWQEINDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYN
                     FASSGEWI"
     gene            complement(2722..3072)
                     /locus_tag="VUW49_001132"
     CDS             complement(2722..3072)
                     /locus_tag="VUW49_001132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981058.1"
                     /translation="MIKIDNTLQYPYSTSAMVLSKYYGVADGMNVEGRGSANFIKDNV
                     LITAAHNYYRHDYGKEADDIYVLPAVSPSQEPFGKIKVKEVRYLKEFRNLNSKDAREY
                     DLALLILEKPIVQN"
     gene            complement(3069..3308)
                     /locus_tag="VUW49_001133"
     CDS             complement(3069..3308)
                     /locus_tag="VUW49_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981059.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3458..3724)
                     /locus_tag="VUW49_001134"
                     /pseudo
     CDS             complement(3458..3724)
                     /locus_tag="VUW49_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3749..4060)
                     /locus_tag="VUW49_001135"
     CDS             complement(3749..4060)
                     /locus_tag="VUW49_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET7981060.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQYYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3985..4227)
                     /locus_tag="VUW49_001136"
     CDS             complement(3985..4227)
                     /locus_tag="VUW49_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET7981061.1"
                     /translation="MSLADLLEGLEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(4228..4860)
                     /locus_tag="VUW49_001137"
     CDS             complement(4228..4860)
                     /locus_tag="VUW49_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981062.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4874..5869)
                     /locus_tag="VUW49_001138"
     CDS             complement(4874..5869)
                     /locus_tag="VUW49_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7981063.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5866..6564)
                     /locus_tag="VUW49_001139"
     CDS             complement(5866..6564)
                     /locus_tag="VUW49_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET7981064.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIIMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6641..7651)
                     /locus_tag="VUW49_001140"
     CDS             complement(6641..7651)
                     /locus_tag="VUW49_001140"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET7981065.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLHTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(7635..8642)
                     /locus_tag="VUW49_001141"
     CDS             complement(7635..8642)
                     /locus_tag="VUW49_001141"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET7981066.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVISLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8629..9582)
                     /gene="mvaD"
                     /locus_tag="VUW49_001142"
     CDS             complement(8629..9582)
                     /gene="mvaD"
                     /locus_tag="VUW49_001142"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET7981067.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(9564..10442)
                     /gene="mvk"
                     /locus_tag="VUW49_001143"
     CDS             complement(9564..10442)
                     /gene="mvk"
                     /locus_tag="VUW49_001143"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET7981068.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10564..10893)
                     /locus_tag="VUW49_001144"
     CDS             complement(10564..10893)
                     /locus_tag="VUW49_001144"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981069.1"
                     /translation="MTRKVYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            11417..12379
                     /locus_tag="VUW49_001145"
     CDS             11417..12379
                     /locus_tag="VUW49_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET7981070.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KVVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
     gene            12422..13621
                     /locus_tag="VUW49_001146"
     CDS             12422..13621
                     /locus_tag="VUW49_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter permease"
                     /protein_id="HET7981071.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQSLAVLLRLKDT
                     VILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMSCGAILGNMTSS
                     NLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGILSPRLQAAVFA
                     HIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLASIFLYLILIALIFCQWL
                     VKFNTHN"
     gene            complement(13739..14695)
                     /gene="cbpC"
                     /locus_tag="VUW49_001147"
                     /pseudo
     CDS             complement(13739..14695)
                     /gene="cbpC"
                     /locus_tag="VUW49_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
     gene            complement(14793..15482)
                     /locus_tag="VUW49_001148"
     CDS             complement(14793..15482)
                     /locus_tag="VUW49_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981072.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLTTLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTIQE"
     gene            complement(15494..16918)
                     /gene="gndA"
                     /locus_tag="VUW49_001149"
     CDS             complement(15494..16918)
                     /gene="gndA"
                     /locus_tag="VUW49_001149"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET7981073.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLNKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(16994..18388)
                     /gene="mapZ"
                     /locus_tag="VUW49_001150"
     CDS             complement(16994..18388)
                     /gene="mapZ"
                     /locus_tag="VUW49_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET7981074.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEELVETQSLDDLIQEMREAVEESEASSEEV
                     PSSEDILPPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTALALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(18401..19558)
                     /locus_tag="VUW49_001151"
     CDS             complement(18401..19558)
                     /locus_tag="VUW49_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET7981075.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKSLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(19643..20023)
                     /gene="rnpB"
                     /locus_tag="VUW49_001152"
     ncRNA           complement(19643..20023)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VUW49_001152"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(20044..20373)
                     /gene="gpsB"
                     /locus_tag="VUW49_001153"
     CDS             complement(20044..20373)
                     /gene="gpsB"
                     /locus_tag="VUW49_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET7981076.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(20443..20970)
                     /locus_tag="VUW49_001154"
     CDS             complement(20443..20970)
                     /locus_tag="VUW49_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET7981077.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFENHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            21036..21632
                     /gene="recU"
                     /locus_tag="VUW49_001155"
     CDS             21036..21632
                     /gene="recU"
                     /locus_tag="VUW49_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET7981078.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            21629..23788
                     /locus_tag="VUW49_001156"
     CDS             21629..23788
                     /locus_tag="VUW49_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET7981079.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAVIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(23955..29258)
                     /locus_tag="VUW49_001157"
     CDS             complement(23955..29258)
                     /locus_tag="VUW49_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET7981080.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANANKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRAIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGATTWALPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLAFNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDSLKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLDAPAQPGEGLENAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRE
                     SGSNGNWRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSVASDQGKTPDYKQEIA
                     RPETPAQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(29559..31541)
                     /locus_tag="VUW49_001158"
     CDS             complement(29559..31541)
                     /locus_tag="VUW49_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7981081.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFLPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         9148 a         7133 c         5655 g         9695 t
ORIGIN      
        1 gcaaaaaagg aaaaaatcgt tactattctc ctgatttaaa acaagaaatg attaataaag
       61 tctgacatga aggctggact aaagatagag tttctcttga atactgcctc ccaagtcgta
      121 cgatacttct taactggcta gcacaataca ggaaaaacgg gtatactatt gttgagaaaa
      181 caagagggag agtacctgag agcggagaat gctatcctaa aaaagttaag agaactccga
      241 ttgaaggagg aaaaagagaa agaagaaaga cagaaattgt tcaagaatta atgactgaat
      301 tttcgttagc tcttcttcta aaagccatta aactagctcg ttggacctac tactatcact
      361 tgaaacagct agataaacca gataaggacc aagagcttaa agctgaaatt caatccattt
      421 ttatcgaaca caagggaaat tatacttatc gccgggttca tttagaacta agaaatcgtg
      481 cttatctggt aaatcataaa agagttcaag gcttgatgaa agtactcaat ttacaagcta
      541 aaatgcgaca gaaacgaaaa tattcttctc ataaaggaga cgttggcaag aaggcagaga
      601 atctcattca aggccaattt gaaggctcta aaacaatgga aaagtgctac acagatgtga
      661 cagaatttgc cattccagca agtactcaaa agctttactt atcaccagtt ttagatggct
      721 ttaatagcga aattatcgcc tataatcttt caacttaacc caacttagaa caagtacaaa
      781 caatgttgga acaggcattc acagagaagc actatggtgg agtccttctt tgggattctg
      841 aaatcggaaa tgttttatgg ctatgagaaa acatttaaat cacttaatca attggaacaa
      901 gctattatag actatattga ttattacaac aacaagagaa ttaaggtaaa actaaaagga
      961 cttagtcctg tgcagtacag aactaaatcc tttggataaa ttatttgtct aacttttggg
     1021 gggcagtaca gaaaaacctt gatactatgc gttttttgat tgtgaagact tttatccacc
     1081 tattatacaa agttaagtat ccatcacagt ttacaattag tcttcattac ttaacccatt
     1141 caccattata gtttaaatag taaccaccta ctgtggtatt aatagctaaa gcacctgaat
     1201 cataggcata gtaccagtta ccattgactt ggaaccagcc tgttttcata tctccattac
     1261 ttgcatttag atagtaccaa gtcgaacttt cctgatacca accagttgcc atagctcctg
     1321 atgaacggag atagtaccat ttgttcccaa ggttttgcca gccagttctc atatcaccat
     1381 tttcagcatc tagatagtac caagttgagc cttccttata ccagccagtt gccatagctc
     1441 ctgacgaatg gaggtagtac catctattac ctagatattg ccaacctgtt tgcataatgc
     1501 cagttgctgg atctagatag taccatggag ctgcttttag cttctcttca tcatacgtaa
     1561 gagggtaatc cttaacccaa ccacgtgcta gctctccaac cgtcaatctg ttgatgcgcg
     1621 aatcaaagcc accatccttc tgtaaataat accaatgacc ctcttcataa atccaaccag
     1681 tttttaaagt atgataacta cgctgatctt caaaataata gactcgtttc tctgcttggc
     1741 tatcatattc ttccccatga tgtttgttgg tattcgtagc agtttttgct tctaaaactt
     1801 gcttgccaac aaattcttgt aatacaccat cttgaccaaa ataaaaccaa tctggtctaa
     1861 gagcaatctc tattcttgga gaaggaccaa tcgtaacccc cttgtgtgga gcagatatat
     1921 attgccagcc gacaaccatc tctccggata aaggatcaaa ataatagtac ttcccatcta
     1981 tcactctcca gtaggtttct tttagatccc cctttttgta gtaggttcta ccattttctt
     2041 ggacaaactg ccatccttca gaatcatctg caaatactgt atttgtcgct aacaaaccga
     2101 agaaaaatgt ggctagtgcg atttgcatca tttttttcaa aagcttcatt ttatatatcc
     2161 tccaacatta aatccactca ccagatgaag cgaagttata aactttacca tcgatagttt
     2221 ggctacctgt aaccattgct ccatttgaat tgagataata ccattttcca tttacttttt
     2281 gccaatctgt aagcatctta ccggaactgt ctaaataata ccaagtatca tttatctcct
     2341 gccaacccgt ttgtttatca tgttgtctat aatggtacca actaccattt attttcttcc
     2401 atccttcaag agagtaacct ttaagaaccg aataaataaa tggcaaattt cgttcattta
     2461 atttaactgc actgttaatt tgattagatc catctcctaa agtatgcact cctactacgc
     2521 ggttactagc atcataaact gtagatccac tagacccctc taaagtatca acttggtagt
     2581 ccaagaacat gccatcatca cttaaaactt gttttttatc tgtatacatt tgatgaattt
     2641 taaaattata tgatggatag cctgtgatag tcacagttat tcctgtcaaa tttttttgac
     2701 tagtaggaag acccaaagtc cctaattttg cacaatgggc ttttctagaa ttaataaagc
     2761 caagtcatat tcccttgcat ccttagaatt taaatttcta aattccttca aataacgaac
     2821 ttcctttact ttgatctttc caaatggttc ttgacttgga ctaacagccg gaagaacata
     2881 aatatcatcc gcttctttcc catagtcatg tctgtagtag ttgtgagccg ctgtaattaa
     2941 cacattatct ttaataaaat tcgcacttcc cctcccttct acattcattc catcggctac
     3001 tccataatac ttagataaaa ccatagctga agtcgaatac ggatactgta aagtattatc
     3061 aattttaatc aaatcatcat taccgataat acttctgatt gcttttggta gtatgaacca
     3121 tatgttggtg aaatctcaga taatgaagaa tcattagatc ctggaccttt ttctagtgtc
     3181 tcacttacct catattcttc acccgtacta gaaataacac tcaaagcaga tactgtcgat
     3241 aactggctag ccaataaagt actcgcaata attgaaatac ccaatttttt ataaacagtt
     3301 ttcttcatta ttgtagcctc ctaatgtaat tatagcgtac tattctaaat ttcttaatct
     3361 actatagaat caagaaatct accaccttct ttaaataccc tccattatca cataaacagg
     3421 taaacttttc aattaatgac tgcgcttttc aatcacgcta gaggtactta cttgcttctt
     3481 tgatactaag ttcagccatt ctttccttgt ttttctcaat aaagcatgtt acccaagtgg
     3541 gattggtttt ggagtagtct cgcagagtcc agccaatggc tttattgata aaaaattctg
     3601 tttggttcaa gttatgaagg agaatctttt ccattaattg agtattggtc ttctcttttc
     3661 ttaacaactg gtggtcaata gcgacacgtc tcagccagat attatctgat aggctccatt
     3721 ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcagattg ctcaaaacac
     3781 tgttttgagg ttgcagatag agctgacgtg gtttgaagag attttcgaag agtattaaga
     3841 ttatttcttc tagttcaggg tgttcataca ccaaactccc tactactcga tctaggatat
     3901 ctaccgtgtc ccaccaggat tttgtcacga ctaactgctc tagcttaggc aaatcggttt
     3961 cctttagata atactgcatt gctttcaaat agttagcagc cacatattgg tattttctag
     4021 gctccttttc ccagcaagtg tctgcaaaat cccaatcgat aatctttgtt tttttcgctt
     4081 ctggaaaata tttttataga gtttatttct ttcaggcacc gcaataccta gaaaagaaaa
     4141 ttgatggcgc atataggctt ccatggatct tgctttttta gagtcttttg ctgcttctag
     4201 cccctcaagt aaatctgcta aactcatcta aaactcctct tgccccacca aatggtgctg
     4261 aaaggcatag acagccgcct gggtacgatc gctgacttca agtttggcaa gaatattgga
     4321 cacgtgggtc ttgaccgtct tgagagagat aaagaggtca tctgcgatgc gctgattttc
     4381 gtagcccttg gcgatgagtt ggagaacatc tcgctcacgc gcagtcaatt cttcatgaag
     4441 ttccatatga ttgcggtggt attcaacctt cttgctaacc tcttgctcaa tggccagctc
     4501 gccagcagct accttactga cggcatgaag caattcatct gcactagaag tcttgagcat
     4561 atagcctttg gcaccagcat ctaagactgg catgattttt tcattgtcca aataagaggt
     4621 cacaatcaaa atcttggctt caggccattc tttaaggatt gctaaggtcg cgtcaatccc
     4681 attcatctca ggcatgacaa tatccatgac aatgacatct ggacgcagtt ccaaggccaa
     4741 gtcaatccct tgagacccgt tggacgcctc acccacaact tctacatcgt cttggaggtc
     4801 aaagtagctt ttcaagccca aacggaccat ttcatggtca tctactagta aaattttcat
     4861 ctttactcct ttatcattcc ttatctaaca ggggaatacg gatatcaact gccagccctt
     4921 gcttgggagc tgttaataac tgaaccgtcc ctgccatatc ttcaacccgc tccttgatat
     4981 ttcgcagtcc ataactcaag tcgtctaagc tccctaactg gaaaccaatc ccattgtcca
     5041 ccaccttcag ttgcaattca acatctgtct gatagaggta gacatctagg caagatgcct
     5101 gggcatggcg gagcgtattg ctaatcaact cttgcaggat acggaagata tgctcctcga
     5161 ttttcttagg caatttcgtc atattctgct tgagactaac cctaagatca ctcttgtcct
     5221 caagctcttt taagagaatt tgaatccctt ctatcaagct cttctgctcc agttcaactg
     5281 gtcgcaaatg caagagcaaa actcgcaaat ccttctgggc tgtttctaaa atagctgtga
     5341 cactctgcaa ctgggtctgc atcttttctc tatccaattt caaagcctgc tgactgatac
     5401 ccgataaaat catgtgggcc gcaaacaact cctgactgac tgtatcgtgc aaatcccgag
     5461 caattcgctt ccgttccttc tcgatgattt cctcttcctg agcaaggcta tgattttcag
     5521 ctttttgaag agcttctgtc aaaaggttaa gtttacctga taaggacttg aaactggcat
     5581 ccaaatctgg atctgcaacc tgaaccactt cttgccctgc caataaacgc ttgagattag
     5641 cctgcatttt tcttagagaa agctcttcga tccctcgcca aaacagggct aagagacagg
     5701 ttatggacat gctgaaaacc aacaataaaa agacaaattt ttctgttttt tcgacatcgt
     5761 gcaaaaagat agaccagtca aaatcaagta tttccagcaa gctgtgggag aaaaaaaaga
     5821 caaacaggaa ggaggtgaga gcaataatga cataggcttg ttttttcatc ctctaaccac
     5881 ctccacatca ccaatcatag tggtcaagaa aatcttgaca ctcttgttac tcttgagata
     5941 gtcttttgtt tcttgatgat agtgttcatt gcggagggct cgcttgggct ggttgaaaaa
     6001 agtcaaatcc ccatagagac agttaacgct gagactgact tccacatcta caggtacgat
     6061 gattttggtc gttcctacca tctttctgag gataatgaca ttgtcatgat tggttaagat
     6121 gaccctctcc agatgaatag tgtccttgcc catgaagcga aagagattga tatcatcgaa
     6181 ttggcaagtc tggtagcttg aaaaatgatg aagatttcca aaccaacgat ttttctcctt
     6241 cttaaccgtc acgacctctt caaaaaccaa attggtctgc tctttttcct ggttcatcat
     6301 cgggtaaaga agaaagaggc tatagataac cgcaacaaaa atagctagaa tcacaaaagg
     6361 attgagcata atgatgaaaa agaagagaat ggttgccgct actaaaagaa gattatttcc
     6421 ctctttacca gtgtagtagc gaatcaaaag caaaaagagg aatagtatca gcagaaaacg
     6481 cgaaaaatgc tctgatacca tcaaaatcag agctcctgtc agaagacagg cttcgataaa
     6541 taaaaagatt ttaaattttc tcataggttc atcctctccc ttctatttta tcacaattca
     6601 aaaaagtcac ctcagtctga ggatggaaaa aaggcgctgg ttacgccttt ttcatctgat
     6661 cctttgcttc ttttaatttt ccataaagaa gatagtctac tttttgtaga tctgctatgg
     6721 tggcacagtt aagggaacac ataatcaagc gtaggtctgc tttccagcct tggacaatgc
     6781 caatcacttc ttcaactgtg taggtttcaa ccaattccag aacggttcgt gacaatccca
     6841 cagccttagc accaaaaacc aagcacttaa tcatatccag cggattccga acccctccac
     6901 taaccaagag ttcgacctta tctttccatt cttgggcatt gagaagggcc tgcatggtag
     6961 actgacccca ttgattgagg taatcacgct ggccactacg acggttttcg atataggcaa
     7021 agctggtgcc accacgaccc gataggtcca ctgtacgaac accgaattca taggctcttt
     7081 cgattgtctt ggcatccatt ccaaagccca cttccttgag gacaatagga acgggaattt
     7141 gcttgctata atctgctaga tgcgattgcc agcttctaaa cttcctttct ccctcgggca
     7201 tgagtaattc ctgcatgaca ttgacatgca cttgcaatag aacaggattc atctcttcta
     7261 cagtatgaag tcctaactcg acaggcttgt ccaatccaat attggttcca aggaggagat
     7321 tgggatgact agacttgaca gaaaaagaat catccgttgg atttttgagg gctgcgctat
     7381 aagaacccgt tacaaataaa ataccacagg attccgccac ctgagccagc ttttgattga
     7441 tttctcttcc cttattactt ccaccagtca tggcattgat ataaaaagga aagtcccact
     7501 ttcgaccagc aaactctgtc gaaagatcga tttcatccag attgtaaaga ggcaaggaag
     7561 aatgaatcag ctccacctca tcaaagctat tataggaact tttctgctca agggcataga
     7621 ggatatgctc atccttacga tttgtcgtca tgtcctatcc tttcttgata taagagctca
     7681 atccccagat cggcccaacg attttttaag gtttcggttg attgcgcatc aaaactcagg
     7741 gcgatgccac agtcaccacc accagcacca ctactcttgg caacggcctg caaatcttga
     7801 ctggcttctt tcaactgtct aagcaaaggc gtgtaaatat ctgtactcaa gccttctaaa
     7861 agcttgctgg ctacttctac ttgctcgata atcttttctg atttcccctg ttccaaggct
     7921 tcgaccaaag aaatcaccgt ttcttttgag gaacttaaaa aattttgatt gatattttgc
     7981 ttgatttgct ggaccatgtg actcgataca gccacttcct tggtccatcc cactaagaaa
     8041 tcacattcta aagttggttt cacttgtgaa attgaaaagc cccaatcacg ctctaaaaca
     8101 gtcgccaagt tttcttcttc taaccaagca gccgccttct ggcgatcaaa tgactggtag
     8161 agaaccaaat cctctgccac aatacaggca aggtcgccca tggaaccatt gtctcctcgc
     8221 ttgagcaaga cagcgctagt cagcttgaac aagagattct gatcaaccga aagattatag
     8281 agagccagta aagccttgac aaccaagaca acgacgctgc cactagaacc tagaccaaac
     8341 tttttccctt ctcgttccat tttgccacag atttctaggg aaaaaggtct taaattctga
     8401 ccacgaacag cgaggaagtc tcccatcaaa gcaatcgttt cttgaatcaa gctatagtca
     8461 ggattgggcc ttaagtccac tgcgaaatca aacatatctg aatagatacg gtagctatca
     8521 gaaaaagcaa tctcagccct catatagatg ggaatatcct ttatcaaagc taactgccct
     8581 ggctctaaaa tagcatattc acctgccaaa tagagttttc cgcaagtttt aacagcaatc
     8641 atcttgactc aaatcctttg tttttgacac aatcaagcga taacgctgac cgaaaatttc
     8701 tgataaatgc tccaagtctt tctcctgaca gaagacctta acattgggac cagcatccat
     8761 ggtaaagtag caggcctctc ctttctcacg aagctggcga acaaagtcca tagcctcata
     8821 agaggcatcc gtcagataag aaaaggctgg actagcagtc tttgtcgtag catgcatagc
     8881 cagggcattt ttctccgtta attctccaat cttggcaaaa tcattttcct tgagataaat
     8941 cagcatatcc tgatagtcct tctcagactg acgaacccag tcgtcaaaag tcgtcgaggt
     9001 ttccacacaa agtttcatcc cgtcacggct agagattggt tttttcttgt cctctagcac
     9061 caacataatc atagctagtt tcaagtctgt ctctacaggg taaatttctc cactatcctt
     9121 atcccaggct cctagtggtc cataaaaact ccgagaagaa gaacctgagg caaatttggc
     9181 ttcctgtgcc aactgacttc tatccaatcc aagcttgaaa taagcattac aagccttgac
     9241 cagggcggac aaaccactag aacttgagga caggcccgct gccgtaggca tattgttttg
     9301 agtatcgata cggacaaagc cctcaccagc tggacgataa cggtcaataa tcttactcat
     9361 cttggcatgc tcgacctcat tttgtagctg accattgatg taaaattcgt cagctgttac
     9421 attggctggt aaaggcgaca aggtcgtctc tgtatacata ttttccaaag ttagagaaat
     9481 actgctagta gcaggcacca tctctttttc ttttttcttt ccccaatatt tgataatagc
     9541 aatatttgcg taggaacgta ctgtcacagg ctctctatcc atgtctgaac agctcctttc
     9601 tcttctaatc tttctgctag ttcttgtgcg tgtgtcaaat tggttaccaa ggctatgata
     9661 caacctccta gcccaccacc gctcatcttg gcacccagag caccatggct aagagtcgtt
     9721 tcaaccaaaa agtctgcctc agggctactg actccaattt cttttaaatg taaatgcgct
     9781 tgactgagga tttgtcccag tccttcagca tctttttgtg aaatcgcaac ttctgcttgc
     9841 tgggttaatt ctcccaaggc atgcaaaaac ggtagggcat ccttgccctt attttgaacc
     9901 acttggatgg cttcacgagt atgaccataa acacccgtat cggcaatcac caaataggcg
     9961 gataaatcca tctcaagttc tgtaaatcct acgttcttga taaagcgaat aggttggtca
    10021 ctgagacagg tcttagcatc caaaccacta ggattcatat gggcaatcat ttcagctcga
    10081 ttgaccaaga tttctagtac atcatgaggc agatcagcct gatagtagtc aaataccgca
    10141 cgaatagccg ctatgctgat agccgctgac gaacccatcc cccgtttctc agggatagcc
    10201 gagtcaatct cacaacgaat gcaggcttct gtgatatcca aatactccag tgaggcataa
    10261 accgccatgg acaaggtatc ctcctcataa aggcgccaag gactctctgc aggaactacc
    10321 ttacaggtca cctccacctc caaaagaggc agggaaatgg caggataacc gtaaacgacc
    10381 gcatgttccc ctattaaaat tatcttacta tgtgcctgac cgacaccaac tttttttgtc
    10441 attttttcct tttactagac gaaaaaacgt cttatttttc atacaagtat taattctttc
    10501 ctatctattt tattatattt tcacaaaaaa agcgattgtt tccattcaca atcgcttctt
    10561 tcattattga acccattcgc cattatagtt gacagaatag ccatctacgg tcgtattcac
    10621 tgccaaggca cctgagctat aagcgtagta ccatctgcca ttgacctgga accaacctgt
    10681 cgtcatagaa cgacgaaaga aactccatac cattaagtaa agaggaaagt cgtgagggag
    10741 catgcgccat tgacaccctg ttttagtgac gtacaaagtc tcattaacaa gtactcgttt
    10801 cggccattta taggtgcggt gtttggagaa atagggttca atcttagccc attcttgatc
    10861 gtttaaatca gtatcatata ctttgcgtgt cataactcta gtttaacatt ttttgtgaat
    10921 acaggttcta aataatcgac cacgaaaatt tcttaagtgg aaaacgcctt atgaagtatg
    10981 ctacgggaaa gttatgcact taatttgaca attcaagatg taaaaatata tactatagta
    11041 gattgaaact agaatagtac acctctactt ctaaaatatt gttagaaatc gatttgactg
    11101 tcctgatcga tttatcctgt tattatctca ttttactata atatttgata agttatccta
    11161 aaagtattat tatgttgttg tgttatagat tgattgaatc taactaaagg atcctattca
    11221 attactagaa ctatcacata ctcaaggtca gctcacatat gagcaactat tttggttaca
    11281 atgtctacta aatttaagtc aaacaaataa tttagtcaaa attaaaaaaa tagaggaaca
    11341 taaatatgat tacaaaacag aatgtaatag tgttctacaa tttttactag ataaaactgt
    11401 aaattctgaa ggaaggatca cttcttcaac agaatttgga aatttcgtaa gtaatttatc
    11461 attccaacac ggaatagctg gactactgtt tcctctaaat aaattgtacc ccccagaact
    11521 ggattctaaa atactctcta tcatcaagaa ggtagtgaca attagaacga cacatacata
    11581 tgaatatcaa tactcactgc tatttggtga tgcaggctat ctatggttac tcctacattt
    11641 attttctatc agtaaaaatc aatactatct acaattagca aacgtcaccg ctaaaaaatt
    11701 aatagagaat tatgatactc tagaggaaat agactttgca ttgggaaaat ctggtgtcct
    11761 attatcatta ataaaatact atcaatttac caatgacaat actcttaaaa ttttcatcca
    11821 caatagtata ggggaaattt atcattattt cctacaaaga gatacagcca aagaaagcat
    11881 tttagactat agctttgctc atggatattg tggaattgca tatgctttat ttgcctattc
    11941 taaagtctta gaaccttcta tgttctataa tgatctccat acattccata ctgaattaaa
    12001 aaaattatta gaaaaagtta cttctaatac tgaaaattta ggaaatttac aactttcttg
    12061 gtgcaaagga atttccggaa taatcttata tctttgtatg tacgattgtg acggaaacaa
    12121 agatattatt agtaaatatc aagaatttgt ttttaaccat catctaaaaa tgatgacagg
    12181 atattgccac ggaataacta gcttactaca aaccactgtc tacaatcaaa acaaattact
    12241 gatgaaaaaa atccaacagg taattttagc atgttctgaa cgagatgatc acggtttact
    12301 gatgtttcaa ggagatagtg gtaaagcaga tttgtttgac ttcggaatag gaagcatggg
    12361 gtatattggt gtctattaaa taataaattc ccattcgatg tgcagacata aggagaaaag
    12421 tatgaaatta ttttggacaa acaacatata tagacagttg ctgctaaaca gctgtttttc
    12481 atcattcggc gacagtattt tctacctcgc cattatcaat tatgtggctc agtacaattt
    12541 cgctccgcta gcgattttac tgatttccat ttcagagatg gttcccctac tatcgcaact
    12601 ctttctcggg attctaggag attttcaaga aaatagagtc aaacacgcac tctggattgc
    12661 caaaatcaaa atcctgctct acgctatttt gacagtattt ctcgtattgt cgcccttttc
    12721 attagtttca gtcattatga ttgtcatcat caacctcatc tctgacacct tgagctacct
    12781 gtctgcctac atgatgaacg ccctctacat cagtgtaatt aaggacgacc tgcatgatgc
    12841 catggggttc aggcagtctc tgatgagggt tgtccgtatt gtcgccaatc tggctggcgc
    12901 attccttatc aacactctga cttttgtcat tgcctttttg ggcctgtatg ttattcgaca
    12961 taccttgtat gaggttgaaa aaagaattga aatgtcacat acagcactga gttttaagaa
    13021 atattttcaa catcttaaac agtcgctggc tgtgctcctg aggttaaaag ataccgtcat
    13081 actactgttt ctgacgacca gtatgattgc catcttggat gtgtcccctc ggctgattgc
    13141 cctccgcttc atccaacaga cactagcaca actgagcatt gggcaactcc tcgccctgct
    13201 ctccatcatc atgtcttgtg gagctatcct tggcaatatg accagcagta atctatttaa
    13261 aaatatccgt ttcacgcacc tcttggtttt ctgtgagatt tccctattga ctctaataac
    13321 tagtatcctt tgtcaagcct atatcgtaat tttcatgacc agtttcatca gttctacgat
    13381 tatcggcatt ctcagccctc gcctacaagc agctgtcttt gcccatatcc ccagtgacaa
    13441 gatggggacg gtgggctctg ctctgagcac agtggacatt ctcgcaccgt ccctgctctc
    13501 cctattagcc ctatccatag catcgggcgt ttcggtgcag ttagcatcga tatttttgta
    13561 tcttatttta attgctctta tcttttgtca atggttagtc aagttcaaca ctcataacta
    13621 acgaaaaagc atgtgtagat ttcacatgct tttaatctcc ccaatagtca ggtcaagtac
    13681 aacaaagtca cttctttgat taagcgagtg ttctaatata attataagcg ccctgtcatt
    13741 accaaaccca ttcgccatta tagttgacgt agtagccatc tacggtcgta ttcactgcca
    13801 aagcacctga gctataagca tagtatcagt tgccattgac ctggaaccaa cctgtcttca
    13861 tatctccatt acttgtattt aggtagtacc aagttgaacc atcttgatac cagccagtct
    13921 tcatatcacc atttttatta tctaagtaat accatttgcc ttgtacatat ttccaacctg
    13981 ttgccataga accagaggag ccaaaataat accattttcc gtttactttt tgccaatctg
    14041 taagcatctt acctgaagca tctaggtagt accatggagc aggtttgctt ctatcaatgt
    14101 caatagtaac atgaaaatct tgagtccaac ccttagcaac ttcaccaatt ggtagtggat
    14161 tgtaagaatc atcgccaaaa tttcctagct tatttaaata ataccaatga ccctcttcat
    14221 aaatccagcc tgtctttaca gcataattat tatcaaaata ataagttctc ttttcagcag
    14281 atagaggata ttgttcaccg tatacttttc ctgtattttc agacgttttt gcctctagta
    14341 cttgtttatc tgtttgttct agcaaagcac catcttgtcc aaaatagtac cacttctcct
    14401 gaaggccaat ttcgaagact ggacgattat caaataaatc atcacgataa cctgttccag
    14461 gaatttctaa gtattgccaa ccgacaacca tctctccaat atcaccaaaa tagtaagttt
    14521 taccgtctat tttatgccaa tagattgctt tatgatcatc ttttacataa tattttctat
    14581 tatccttatc aacaaatcgg ccacctgtgg tattcgcaaa taccgtattt gtagctagca
    14641 aaccaaagaa aaagactgct agtagaactt gcatcatttt tttcaaaagt ttcatctata
    14701 tatcctccta aaacgattgt agcaaagacc cgactgcatg tcaatatata aaaactcctc
    14761 aaaaaagcag ttacacaact gcaactgctt atctattctt gaatggtata tccaacacca
    14821 cgcacggttt taatgtagct tttttggcct ttaacatcaa gcttgctccg tagatagcgg
    14881 atatagacat ctacgatatt tgtctcggtc gcactttcat acttccaaac actttccaac
    14941 aattgctcac gagtcaatac tttcttgctt cccataagtg tcgtcaaaag gtcatactca
    15001 cggcgggtca gagcaatcat ctcttcacca cgataaaccg tgtgatgttc aacatctatc
    15061 ctaagattgc ggtaggtcct tggaactttc atcagactac agtgttgatc aatgaagtcc
    15121 cgacctcgga agatcgccga aatacgcgct accagatttt cgataaggac tggcttatag
    15181 atgtatgaaa ctgcaaaacg ctgaacaact tccagctctt cttgcaagtc ttcccaatga
    15241 tctaaaatca tgatgactga ggcaggttta gttcgactca atttttctgc aaaatcctga
    15301 gccatcatat ctcccagatt aacgttcaat aaaatcaaat catagtctgt ctgaagagcc
    15361 atggagaggg ctttttgccc ctcctctacc agatcaaccc gatactgctc tttctggagt
    15421 tccaaactta aaaaatgagc tagatttcgt tctttctcaa gtaataaaat ccgtttcccc
    15481 atggctgacc tacttatttt tcgtcatacc aagagtagtg gaaggttcct tctttgtctt
    15541 tacgttggta agtgtgagca ccaaagtagt cacgttgtgc ttggatcaag ttagctggaa
    15601 ggtcagctga acggtagcta tcaaagtaag taatagctgc tgagaaagtt ggcactggca
    15661 cacctgcttg aaccgcaaga gctacgatat cacgtactgc ttgttggtac ttagcagtaa
    15721 catccaagaa gtactcgtcc aaaagaaggt tggcaagatc tgcatcacgg ttgtaagcat
    15781 ctgtaatctt ttgcaagaaa cgagaacgga tgatacagcc atcacgccag atagatgcga
    15841 tatctgcaaa tggcaagttc cagttgtttt ctttagaggc tacacgcaat tgagcaaatc
    15901 cttgtgcgta tgaaatgatt tttgagaagt aaagggcttg acggatcttt tcaatcaatt
    15961 cagccttgtc tccttcaaag ttgaaggcag ctggttttgg aagcaccttg ctagcatgta
    16021 cacgttcttc tttgtaagtt gaaatgtagc gtgcaaacac tgactcagta atcagtgaca
    16081 atggtacacc aaggtcaaga gatgattggc tcgtccattt accagttccc ttgttacctg
    16141 cagcatcaag gatatagtct acgattggtc catcttgacc ttcatcgtct ttacggctca
    16201 agatatcagc tgtgatttcg atcaagtagc tgtctaattc acccttgttc cactcagtaa
    16261 agatttcagc catatcctct gcagaaaggc ctagcaagtg ttgcatcaag tcatagcttt
    16321 ctgcgatcaa ttgcatatca ccgtactcaa taccattgtg aaccattttc acatagtgac
    16381 cagctccatc aggaccgatg taagtcacac atggcttgcc atcttctggt gctttagctg
    16441 agatttcttc aagaacatcc gcaaccaatt cgtaggcctc tttttgtcca ccaggcatga
    16501 tagaaggacc ttcaagggca cctttttcac caccagaaac tccagtaccg ataaagttga
    16561 tacctgagtt tgccaattct tcattacgac ggatggtatc tttgtagaaa gtatttccac
    16621 cgtcaatcaa gatatccccc ttgttaaggt gtggaagaag ggcttggata gtagcatctg
    16681 taccaggtcc agcttgaacc atcagcatga tacgacgagg tttttcgatt gagtttacaa
    16741 aactttcaac gtcatagctt ggtacaaaat tcttttcagg atggcaagca atcacatctt
    16801 ccgttttttc tttactacgg ttgtagatag caactgtgta accacgtgat tcaatattaa
    16861 gggcaaggtt acgacccatt acggccatac ctacgacacc aaagttagct tttgtcattt
    16921 gacactcctc ttgtttcatt tgttttattt tatcattttt acttttgaaa agaaagaatt
    16981 ttgtaacgac tgcttagtag tccaagtcat ccgcatgacc agcgccattt ccgacaaagt
    17041 agcctgtctt acagttaagg gtaaagagat aggttccatc tggcttgtag aggttgtaat
    17101 aaccattgcc gttaacgata ttgacacgtt caaggatata ttggtctcca gtgatataac
    17161 cacgtgaacg tgaagtcgct agaatttgtt ccaagacacc atccgcaaaa tcccaggcag
    17221 agttattact atcatcaata gcagactgat tataggcaac acgactggca cttctttgaa
    17281 cagcaacccc tgcactcgag agacccatat tgacttcact gcgactactt ctagtctcat
    17341 ttgaactact tggttttggc tcactagtcg tatttgaact tgcttgacta gagcttgagc
    17401 tgctagtttg acttgaactt gagctagaag tacttgtttg ctggctctta ccaagactga
    17461 tagccttatc tagcacttta tcaagctccg tatttccagt tttaatatcc gtaaatttag
    17521 catccgattt ggctttggca ttggtatcca acacaccatc cacaatagct ggtttttcaa
    17581 attgtgcatt gacatcttga atagccttga tttgcgttgc tagactatca tatttagatt
    17641 tggcaagcgt atgttcacga ctaccttcca gtttatcaag caaggttttg agttgactca
    17701 gtttatcaaa ctggctattt ttcaaagccg ttttattgct atctgtgtaa aaggcgtcat
    17761 aaagtgtatt aaaatcatcc acatccgatt gattggctgt agttgattga gaagtttcaa
    17821 tttccttagt cgaacgagcc acttgacggt agacataata agcactgaca caaataatta
    17881 ctgatacaag cgccaaagcg gtcaaaataa agcctttttt acttttcttt tctgagtcct
    17941 cttgatccag acgagaaagg ttttgctcct cttccacttc ttcagtcatt tctgttggat
    18001 tttcatcatc tagcaataga ggatccaagc cctgctcctc atcgtccaga ggcaagggtg
    18061 gtaagatgtc ttcagaagat ggaacttcct ccgaagaagc ttctgactct tctacagcct
    18121 cacgcatctc ttgaattaaa tcatccagac tctgagtttc aactaactcc tcttttttgt
    18181 attgacgagt cgcaaactta tccgcctcaa tttcatctct gtgttgctta acatacttgt
    18241 ccaaaatgga atcctcaggc aagactcctg cttccacttc ttcattttta cgaatagctt
    18301 gaccaactgt tagctctttt gcttcatcaa aatcaaattg cggttcttga ccttcttttt
    18361 tatgacgatt tcgtcttttt ttactcatga gtatcccttt ctattttacc tcttgccgtt
    18421 tgacacgctg accaaaatat tgatatagat ccactttcaa ggttccgttg tataacttac
    18481 gcttcttatc tgcttggcta ccatacttgc tttcaaaagc ttcatcacta gtcaggataa
    18541 atttgctcca agttttcagc ggtgcaaata cttgccccat ctcagcatag agcttggtca
    18601 cccctgcatc atctgacaaa cgttcaccat aaggcggatt ggaaatgatt actccattga
    18661 ttttatcgga acgtaaatcc tgcacgcgca tctgcttaaa agtaatgtct cctgcaacac
    18721 cagctgcctg agcattggcc ttagcaattt ccaccatgcg agcatcaata tcacaaccca
    18781 tgatatccag ctcaagctca cggtctactt ttttagccgc ttctgtgcgc acttcttgaa
    18841 tcaagcgatc gctgatccag ttccattcct caaatgcaaa agagcgacga agacctggcg
    18901 ccatctttct agcaatcata actgcctcaa tacagaaagt ccccgaacca caggtcggat
    18961 caatcaaaga cttgtctgga taccagttag aaagttgtaa aatggctgct gccatatttt
    19021 ccttgatagg agcgccacct ttttcggtac gataaccacg tttaaagagg ctagacccgg
    19081 tcgtatcaat catgacagtt gccacatctt tgagaataga gacctcaatc ttaaactctg
    19141 ggccattctc catcagagga accccttctg ggcgagcata gtgtttctgc aatttcttga
    19201 caacagcttt cttagaaata gcctgaacac tgggctcatt gtgaagtttg gacttaacac
    19261 atttagcttt tgaaatcggg aaccgagctc caagtggtaa ataattttcc caatctaaag
    19321 cgaaaactcc ctgaaatagc tcttcaaaag tcttagctgg gaacgttcct acgataattt
    19381 tgatacgatc tgctgcccga agccaaaggt tggtttcgat aatagctctc acgtctcctt
    19441 gaaaacgaac acgtccattt tcaacctgac aatcgtagcc caactctcgc acttcacgac
    19501 caacgacagc ctcaagccct gctgccacag ttgcaattaa attaaattct tttttcatgt
    19561 tacagtcttg caagaccttt ctatatgtcc actttaaaaa atgaggttga gaaaatttct
    19621 cagccccaac ctatatgcaa ttttctataa gccatgtttt gttccagaag tgactaggac
    19681 cacttccttc gataatcatc tgtctaccac taacagctct agctgatagc agtcagaata
    19741 ctacgttcgt ttccgcgtac tccatgcccc gaccaaaatt tgggttgcta gcttgagggg
    19801 tttaccgcgt tccactctct ctgtttccag aaagactccg tcactgtggc actttcaagc
    19861 ctactctggc ctatccaagg acttagccat ttcaactgcc gtaacgattt ctcgtcccta
    19921 ggcttatggt ttcgcctagc acaaacacta caggcatcac agcctgtgct agcatggact
    19981 ttcctcatga gaagcaattc tcctcacgcg attatccaaa aattgcacat ctcaaataac
    20041 tacttaaaaa tctgagttat ctaaaatttg tttaccaaaa acttcttttt ccaatctatt
    20101 caggcgtttc aaaatatcaa aattcgtcat agaacttgta attgccgctt caaggggttc
    20161 tgcttgaact ggtgaaggtt tcggtttacg agttaattct tccttcaaat ccgcaatttc
    20221 ctgacgaagt gacttgacca aggcagcata ggtttcatag tccttgatga catcgtctaa
    20281 aaactcgtca acttctactt tattatagcc acggacttca cgtccaaact cttgttcaaa
    20341 aatatctttc gctgaaaaaa taatacttgc catgtctctc tccattctcg cttgctagta
    20401 ttattatata aaaaaaggca aacaaaaatc aaggtcaaag cttcaatttt cggaaaaatt
    20461 ttcagcaagt tcatttaatt catcaaatgt taatctcttt ataaaatagt cctcttgatt
    20521 tttcatcttt tggtaaaaat aagctagttt tgtttcattt tcttcatcat aaaaaagata
    20581 ggaactagtc gtgttttcca gcaaaaactg ctgataatct cttaactgcc ccttgtgttc
    20641 ataacgagga taagcatatt tgacaaagtc tacctgctta aagcgactca gtttcatctg
    20701 attgccttca ttccaatttt ccccatggtt ttcaaaagca aaaatggtgg ccaactggaa
    20761 accgtattcg gttttcatct cctgagcaac ctctaaaacc caatattcaa aacccaaact
    20821 tcctgtaaac acaagccaag acaccccatc tgctgccata gcctctaaat ctttacgtat
    20881 cgctttttta atcaatttaa gacgaggatc cttatcagaa aataaaccca aatcaaaagc
    20941 agaatagcct aaaaccaaag ctgtagccat ttttaaccct ttctgtgcaa attatggtat
    21001 aataaagtga tgttattgta ccaataagga gaattatggt caactatcca cataaagttt
    21061 catcacaaaa aagacaaaca tctctttctc aacccaaaaa tttcgcaaat cgaggaatgt
    21121 cttttgaaaa gatgatcaat gctaccaacg actactattt gtctcagggc ttggctgtta
    21181 tacataagaa accaactcct attcaaatcg tacaagtgga ctatccacaa cgaagtcgtg
    21241 ccaagattgt tgaagcctat tttcgacaag cttcaacgac ggactattct ggcgtttata
    21301 atggatatta catcgacttt gaagtcaagg aaacaaaaca aaaacgtgcg attccgatga
    21361 aaaattttca tccacatcag attcagcata tggaacaagt ccttgcccaa caaggaatct
    21421 gctttgtcct tcttcacttt tcttctcagc aagaaaccta cttattgccg gcattcgatt
    21481 tgattcgctt ctatcatcaa gataagggac aaaaatcaat gccacttgaa tatattcgag
    21541 aatatggata tgaaatcaag gctggtgcct tccctcaaat tccttatctc aatgttatca
    21601 aagaacattt attaggtggt aaaacaagat gaacaaacca acgattctgc gcctaatcaa
    21661 gtatctgagc attagcttct taagcttggt tatcgcagtc attgtcttag gcggaggagt
    21721 ttttttctac tacgttagca aggctcctag cctatccgag agtaaactag ttgcaacaac
    21781 ttctagtaaa atctacgaca ataaaaatca actcattgct gacttgggtt ctgaacgccg
    21841 cgtcaatgcc caagctaatg atattcccac agatttggtt aaggcaatcg tttctatcga
    21901 agaccatcgc ttcttcgacc acagggggat tgataccatc cgtatcctgg gagctttctt
    21961 gcgcaatctg caaagcaatt ccctccaagg tggatcaact ctcacccaac agttgattaa
    22021 gttgacttac ttttcaactt cgacttccga ccagactatt tctcgtaagg ctcaggaagc
    22081 ttggttagcg attcagttag aacaaaaagc aaccaagcaa gaaatcttga cctactatat
    22141 aaataaggtc tacatgtcta atgggaacta tggaatgcag acagcagctc aaaactacta
    22201 tggtaaagac ctcaataatt taagtttacc tcagttagcc ttgctggctg gaatgcctca
    22261 ggcaccaaac caatatgacc cctattcaca tccagaagca gcccaagacc gccgaaactt
    22321 ggtcttatct gaaatgaaaa accaaggcta catctctgct gaacagtatg agaaagcagt
    22381 caatacacca attactgatg ggctacaaag tctcaaatca gcaagtaatt accctgctta
    22441 catggataat tacctcaagg aagtcatcaa tcaagttgaa gaagaaacag gctataacct
    22501 gctcacaact gggatggatg tctacacaaa tgtagaccaa gaagctcaaa aacatctgtg
    22561 ggatatttac aatacagacg aatacgttgc ctatccagac gatgaattgc aagtcgcttc
    22621 taccattgtt gatgtttcta acggtaaagt cattgcccag ctaggagcac gccatcagtc
    22681 aagtaatgtt tctttcggaa ttaaccaagc agtagaaaca aaccgcgact ggggatcaac
    22741 tatgaaaccg atcacagact atgctcctgc cttggagtac ggtgtctacg attcaactgc
    22801 tactatcgtt cacgatgagc cctataacta ccctgggaca aatactcctg tttataactg
    22861 ggataggggc tactttggca acatcacctt gcaatacgcc ctgcaacaat cgcgaaacgt
    22921 cccagccgtg gaaactctaa acaaggtcgg actcaaccgc gccaagactt tcctaaatgg
    22981 tctcggaatc gactacccaa gtattcacta ctcaaatgcc atttcaagta acacaaccga
    23041 atcagacaaa aaatatggag caagtagcga aaagatggct gctgcttacg ctgcctttgc
    23101 aaatggtgga acttactata aaccaatgta tatccataaa gtcgtcttta gtgatgggag
    23161 tgaaaaagag ttctctaatg tcggaactcg tgccatgaag gaaacgacag cctatatgat
    23221 gaccgacatg atgaaaacag tcttgagtta tggaactgga cgaaatgcct atcttgcttg
    23281 gctccctcag gctggtaaaa caggaacctc taactataca gacgaggaaa ttgaaaacca
    23341 catcaagacc tctcaatttg tagcacctga cgaactattt gctggctata cgcgtaaata
    23401 ttcaatggct gtatggacag gctattctaa ccgtctgaca ccacttgtag gcaatggcct
    23461 tacggtcgct gccaaagttt accgctctat gatgacctac ctgtctgaag gaagcaatcc
    23521 agaggattgg aatataccag aggggctcta cagaaatgga gaattcgtat ttaaaaatgg
    23581 tgctcgttct acatggaact cacctgctcc acaacaaccc ccatcaactg aaagttcaag
    23641 ctcatcatca gatagttcaa cttcacagtc tagctcaacc actccaagca caaataatag
    23701 tacgactacc aatcctaaca ataatacgca acaatcaaat acaacccctg atcaacaaaa
    23761 tcagaatcct caaccagcac aaccataaca tttatcaccc agatttttct gggtgatttt
    23821 gtcatagcac tattgtattt atgtgcttaa caaaagcctg agaagacatc ttctcaggct
    23881 ttttctgtac tataaacctc attggaaaag atagagactg cttccgtaat cttgttaaga
    23941 ggttttacta aatactagtc tttcttcgtt tttactacaa agagagcaga tagggctagg
    24001 ctaacacctg ctaggaagag ggctgtgtca gattgactct cacctgttgc tggcaagatt
    24061 tgtgcaggtg tttctggtct agctatttct tgcttataat ctggcgtctt accttgatcg
    24121 gaagctacag agctaagttt aaactcaggt ttctctacag ttggagcatc tggtttcgta
    24181 gcagaatctt ttaattggtt gagataatct gcaaagtatt ccaccattct ttccgtcaag
    24241 agatggttat ccgtcgcata tttcatgctt gcctgaacgc tagctacttc ctcttgattg
    24301 tctttgtctg ttaatttctg agccttagcc aaagctgctt catagcctga caagtcaaga
    24361 ggtgtttctg ctacctgtgg acgagtaaag atgagttccg ctgcagattg gtatttatcg
    24421 ccaccatctc cgtaagtctt agtacctgta aggacaattt tcttagcctt aattgtctta
    24481 ccaaaatcaa tgtcttttgg cttattgtta tctggccaat cagttgcagt aaaggtatgc
    24541 tccttgccag actcatctgt cacaacaagt ttcacatcac gccagttacc atttgaacct
    24601 gattcacgtg gaacataacg aagtccagtg atttcagttg gttctttcaa gaccatggtt
    24661 gcaggcttgc ctacatctcc accattccaa gatgtatgcc atagactaga cacattgcca
    24721 tcaaaggcat tctcaagacc ttcacctggt tgagctggcg catccaaact tgcaaagtca
    24781 tctgctacca aagccgtttt cttctgaacc aaagcattct tcaaggcttc aatcttggca
    24841 atctctgcac gcgcttcttc cacactgata tcatcatcgg cctgactgag attaaagacc
    24901 gcctctttca aagaatccat agactctttg gtgtagttag tcatggcaac cgttggcaag
    24961 tagttcttca gagcattttc tgtcaacatc ttacctgtta gggtaatttc ttcgatttga
    25021 agattatcca tcatgaagtc gttataacca cggaagttgg catttccacc agaatcacca
    25081 cgagtattac ttgcatttcc agttgagtag atacctaccc aagtatctcc tgtttctgca
    25141 cctgtcacga ggaaggttac cttcttggct ttcttagaat ctgtccaagt atttggcaat
    25201 tcatgcattt ccaagttgct tgcttgagta ccacgacgac ctgactggaa ttctccctta
    25261 ccgactacaa aagcataggt attgtctgaa cctgcttcgt attcaaaggt tacacggtag
    25321 gtcttacctg cttcaaaacg gaagttttgc ggaatagttt ggtaaaccaa gttacgacgg
    25381 ctcactagtc catttgtctt gagtgaccaa tttccttcga taacatcatc gactttctta
    25441 ccattccaac cacgttgtgt atatggatcg tgtttttcag acaagtgagt acggttgtct
    25501 tcgacacctt caacaccacc cactacaaat gggaagatac cctgagcaac attttcaaag
    25561 tcttgcttga aggtgccttt acctgtatca tgcttgtctc cgtacatgct tgaattgttt
    25621 tcaaaggtac gaatttcatc aaagtaagtt gcttcatcac cagcttcacg actcaatgtc
    25681 agagtaacat ttgatacgtc cgatccagtt gtaaagaagg cgtacatgtt ttggaagtaa
    25741 cttgtatcgt caactgtagc attgttacga cgtgtattgt gggcgtaggc ttttacatag
    25801 ttgaaggcga gagacttatt ggtataagta gtcacttctt tttcaccggt attcacagtg
    25861 atacttgcct tggcattact acggttatcg acaccgacat aaacggcata cttagtattt
    25921 ggtttcaagc cagttaattt ctgagtgaga ctaacttttt ctttgtttcc ttgaatacga
    25981 agcatatcgt ttgccccttg agacttgaca atttctgcct tagaagcatc accagaaatg
    26041 gtccagtgtt tcaaggttcc actattaaat ccttggtcat agatgtgcat gccttcgctc
    26101 catgacattt caggattggt ttgtttcgaa cgatagagaa cgtatggttg atttgctaga
    26161 agatctaggg taattttacc atcttttaca gttagttctt gctcttctgt cttaccttgg
    26221 tcagttagct tgtaaaggta aaccttgctc tttgcccaat cgcttggaag ggcccaagtt
    26281 gttgcaccgg cctgcgtatt gaagtagtac atcttttcct tctcagtaga aagtttctta
    26341 ccatttgcat cccagttcca aggagtcaag taagctgaac catcttggat ggcacgtccg
    26401 ttgagagtta ctgtacgttc gcgatattgt gggctattga catcatttga cttacgagtt
    26461 acaactactt tattattgtc agcatctacc aattccactc gcatttctgg agtccattta
    26521 taggtgctac cgttatcagt catagtcacc ggtgtaccat tttcccattt acttacagtg
    26581 aagtgttgga agtacttggt catgacgtca tgggcaaata agttggttac atagccattg
    26641 tagtcacttc ttccttgcca gccttcaaag tctttcatgc tgtagccacc tagcagtgga
    26701 tagttggctg caccaccata acttctgtag tccccaaccc aagcatcttt ttggtggtta
    26761 cggataaagc gggtgatggc actgttgata cctttattgg tgtagccacc gtaggtcaag
    26821 tcagctgccc agtgatggaa ggtagagtcg tactcaccac catggcccca ctcgatcgca
    26881 aagcgccagc cttgtttgtt aatttcttta gcaagaacgt gggtagccca ggcaccgtta
    26941 tcacctgatt gaccattacc ccaaacgtcc acatagataa agtcgagacc gtcaccaagt
    27001 tttttcttca aatcttccca acgtgccaaa cgaccatgag ctaggtcata ggcagcatca
    27061 atgttgatac cttgatctag ccagttccaa ccatagctat agcttccatc tggattctta
    27121 cggagaattt tttcattgaa gtatttagac tcaggataag tttctgaagc gttaacgtgg
    27181 atacctagat gagctccata tttcttagcc ttctcaatta gggtcttgaa gtcttcgaca
    27241 ccaccgatac gcttaccaat atcagcatag ttcaagtgac cagagtcatg gccttcgcta
    27301 ccatatcctt taaggagaac accttgccca agaccatctg tatggagatt gattttcttg
    27361 ataccatcca aggtcataag gaatgggttt tgtgcttgag aaccaaagtt catcgcgata
    27421 cggtaagctg tgatatcctt aactttttcc caaccttgag gattgttcat aatgctacga
    27481 taagcaatgg caccatcctg ccaatcaact ttcttgtttg cattggcatc ttcagtgata
    27541 acaaccttag cacttggaag ttccttcgtg tattctggga aaacaatgcc cttataagct
    27601 ttttcccatt gccattcaga gctgtggatt cctacatagt tggcatttcc gactgtttct
    27661 ttataagctg tcaaacgagt ccagtcattc gaaccaccac catagctatt ttgagagtta
    27721 ctccaaacac cagcagcaag cttatctgta gaaacaaatc catacatgta acccttagcc
    27781 aaatccttca ttggattggt tacatcgata tgatcatctc cgctgacatg cgtattgttt
    27841 gacatggttg ccccatcaaa cttagcacca gtttgatcac tagaaacaga gactaaagca
    27901 ttgccgagga aactaataga agaaagtagt tttctttcgt catcaatctt ttgacctgga
    27961 gtgacttgat tgtggttgac aatcttggtc acatcaaagt gcaattgatt gtccacaact
    28021 tgcaagcgta ctgtcatttc cgcattgatt aagtgagcat catcgcgaag cttcatcaag
    28081 tactctgctg ttgtctcatt gattttctta taagtgactt caggggtgat tcggtggtta
    28141 ttgataaaga cttggttgaa ctgttgcacc tgtcctggca aagtatgtcc attcaaggtg
    28201 tattccttga cacgagggaa ggcttggtca atcactgctt tgaggacctt agactgaatc
    28261 gtgtcataag tcaccttgct atcatcaact tcaggacctg tttctttttc agcaggggta
    28321 tcctctgttt ttaccccctc ttggttatcc gttttaacgc taacaactgt tcgctcatcg
    28381 tcataagagc ccgccttgag aagaatcttc ttctcatttt taagatggtc attgaccgca
    28441 gctggtagag tcactgtgtc aaagagattg acatcgttat tgctggcatt tagctgaccg
    28501 tctgacttga gagtgataga aagacggttt attgatcctg tttcaggagc agcaacacgg
    28561 ttgcctttat accatgtgct attacctgga gttttgtatt cccagaacca accatccttg
    28621 tcataaccga caaaaacatt gttcttggta tctttaaatt tcaggaagac accaaagcgt
    28681 gatttgccct tttcagaatc ttctttgaag gttaaatcaa cagttgcatt tccattggca
    28741 tcaacggtca agcccttctt ttcaaacaga gctggtttac ctgcgttatc attttgagca
    28801 gttgaggata gttggttgta gcggacacct ttttcttctc ggatagtgac tgttccctgt
    28861 tgttcttttt tctctaccgt ttgccattca ggggttaccg tcttaggcgt ttcaggttta
    28921 gcagctgcag gtttatcctc ttttggtttt tcttcagccg gtttttcttt ttcaagtgct
    28981 aatagttctt cttctgtctt aggtccatct gtaacttctg gagaaccttg gtcttctccc
    29041 accttaggcg cttcaaaatc acgcccatca ttctcttttg ctgttgcaag agcagtagct
    29101 aaatcagctg gtaagttcgc cgtggaacca gactgcacgc tatctgcaag aaccggactt
    29161 gtcccaaaga atgcagctcc aatcataaca gaagcaacac ctaatgaaaa tttccgaata
    29221 ctatatttac aacgtttttc aaataatcct ttattcataa acttctaaat cctttcattt
    29281 ttgagcaagc gtttacattt tctttttaaa aaatactcct tcctctgaaa gtgcaatgct
    29341 taaccctgat aaataaaaaa taattatact tttaatataa caaataatag ctgagaaagc
    29401 aataggaaac aagtaaaatt aacaaaaatt ttctctattc tgacaaaaag aggctccaac
    29461 ttttgggggt ccgtacattt agaatctttc tttacaaatg taaaaaagga ttcagcattc
    29521 aagggaaata ctaaatcctt tcttatattt tgcaacagtt atttcacatg ttttgcgaga
    29581 tcttcttgag cctttttatt agactcttct ttttctttca tccatttttc ctgagctttt
    29641 tggtattcat ctacagtgac tgccttgtct tgaagttcca agtatttata caagactggt
    29701 tcacttgtac ctttatttcc tgacaatgca aatggtattg taaatggtac catcttagac
    29761 aagattggac gccctgtacg agatgtagtt ggaataatca aagcactatc tgtcaaccaa
    29821 gcttgggctg cagcgtattt atcatagcgt ttagcaacat ctgtagtctc atcaccagcc
    29881 tcagtaacca atttttcgta gtcatataga cctacttttt tagcagctac attatcttcc
    29941 cctgagtcaa accctaaata tgttttagta ctttctccta cagatggttt gatgatatca
    30001 aggtaggttg atggatcggc aaagtctgga ccccaaccga cattatctga taaatcccag
    30061 tcttcgccag cagcattttc agcaaaatat gtaatattgt ttacttcgtc tttttgtagt
    30121 tgttgaatat caatgactac attatccgct cctaaagttg cttccaagga ttgtttcata
    30181 gattggacgc gctgaacttt tgtagttgct gtttggtcaa ctggcatatc caaatgaatt
    30241 gggaatgtca caccttctgc ttgtaaggct gatttagctt tagcaaattc agccttggct
    30301 ttttctggat tgtaaagacc atcctgagaa tctgcaagat taacatcctt ccattcatcc
    30361 ccataagtaa ccaatttctc tttgaccata tcgccaaagt ttttaccatc tgcttgaaca
    30421 aatgttggtg gtaaaaagag attacgcaag attttacttg ctccagtttg tccattcaac
    30481 tgagaggcat aggctgtacg gtcaaatcca aatgcaatag cctgacggaa atccttgttt
    30541 aagagagcct ttttagtcga tgccttttgt tcgtcgctgg tcttagatgt gtatttatag
    30601 gactgacggt caatatttgt accaactaga tacgtaatag agtcttgttg agtatagaca
    30661 atattgtcct tcatactctt ctcaagctct gcgaaacttg cacttgttgg atagagacga
    30721 gctgctgtaa ggctaccatc tttaaagttt tctgcaggtt tgctggtatc ttgaccatcc
    30781 cagaatgaca atttaacttt gtcaatatgc acattgtcct tatcccagta gttcggattt
    30841 ttcgcaaatt caacagaaga tttggtcaca atggatttca acaaataagg accgttatac
    30901 aagagactac ttggatccgt agctttggca aaatcatctc cttttgaatt caaaaactct
    30961 tcattaactg gcgcaagcac acccatggtt gtcttagaat tccagaagct ttctggtttg
    31021 ttcaaagtgt actgaactgt ctgttcatcc agagccttaa ttcctacttg tgagaaatct
    31081 ttgatttccc cttttacata ggcatccaac cctttgattg attcttgaac aaggtaaaga
    31141 gcatctgatt ttttatcagc agcatatttt agtcctgtta caaagtcttg agctttgact
    31201 gccgcgtatt cttccccttc agaagtatac cattttgcat ccttacggat agtataagtg
    31261 taagtcaatc catccttgga tacagaccaa tcctcagcca tagacggcac aaagttcccg
    31321 tagcgatcat tttctagcaa accatcaacc acgttactgg taatatttgc tgtcgcagcc
    31381 ttagcagttg tcaaatagtt gaggttatca gggtctgtct cataaatgta tgagaatgtc
    31441 ttctcacctt tagcgcttga acctgatcca gagcatgcag ctaaagtagt cgccgccaat
    31501 aatgtcacgc cagcaagggc aaatagtttt gaacttttca tcattttctc ctttaaaact
    31561 ttctctccat tatagactct tttcagaaag ttgtcaacag aattttcaga attttaagag
    31621 ttatttgtta t
//
LOCUS       DAVDWM010000021        31334 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVDWM010000021 DAVDWM010000000
VERSION     DAVDWM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.021"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            276..2429
                     /locus_tag="VUW49_001159"
     CDS             276..2429
                     /locus_tag="VUW49_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET7981082.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGEMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2442..3791
                     /locus_tag="VUW49_001160"
     CDS             2442..3791
                     /locus_tag="VUW49_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET7981083.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            3961..4668
                     /locus_tag="VUW49_001161"
     CDS             3961..4668
                     /locus_tag="VUW49_001161"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7981084.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNTAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4670..4813
                     /locus_tag="VUW49_001162"
     CDS             4670..4813
                     /locus_tag="VUW49_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7981085.1"
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQ
                     GLK"
     gene            4870..8595
                     /locus_tag="VUW49_001163"
     CDS             4870..8595
                     /locus_tag="VUW49_001163"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET7981086.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8688..10130
                     /locus_tag="VUW49_001164"
     CDS             8688..10130
                     /locus_tag="VUW49_001164"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7981087.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10167..11189
                     /locus_tag="VUW49_001165"
     CDS             10167..11189
                     /locus_tag="VUW49_001165"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET7981088.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11186..11731
                     /gene="purN"
                     /locus_tag="VUW49_001166"
     CDS             11186..11731
                     /gene="purN"
                     /locus_tag="VUW49_001166"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET7981089.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGHVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11815..12324
                     /locus_tag="VUW49_001167"
     CDS             11815..12324
                     /locus_tag="VUW49_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7981090.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12349..13896
                     /gene="purH"
                     /locus_tag="VUW49_001168"
     CDS             12349..13896
                     /gene="purH"
                     /locus_tag="VUW49_001168"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7981091.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14018..15280
                     /gene="purD"
                     /locus_tag="VUW49_001169"
     CDS             14018..15280
                     /gene="purD"
                     /locus_tag="VUW49_001169"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET7981092.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVADTV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            15683..16171
                     /gene="purE"
                     /locus_tag="VUW49_001170"
     CDS             15683..16171
                     /gene="purE"
                     /locus_tag="VUW49_001170"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7981093.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16158..17249
                     /gene="purK"
                     /locus_tag="VUW49_001171"
     CDS             16158..17249
                     /gene="purK"
                     /locus_tag="VUW49_001171"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7981094.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17259..17486
                     /locus_tag="VUW49_001172"
     CDS             17259..17486
                     /locus_tag="VUW49_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET7981095.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17549..18847
                     /gene="purB"
                     /locus_tag="VUW49_001173"
     CDS             17549..18847
                     /gene="purB"
                     /locus_tag="VUW49_001173"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7981096.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18902..22840)
                     /gene="strH"
                     /locus_tag="VUW49_001174"
     CDS             complement(18902..22840)
                     /gene="strH"
                     /locus_tag="VUW49_001174"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET7981097.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYDDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERELQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNQQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(23154..23870)
                     /locus_tag="VUW49_001175"
     CDS             complement(23154..23870)
                     /locus_tag="VUW49_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7981098.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIEFISCDRDH"
     gene            24218..26005
                     /locus_tag="VUW49_001176"
     CDS             24218..26005
                     /locus_tag="VUW49_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET7981099.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYIAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEERGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26002..26478
                     /locus_tag="VUW49_001177"
     CDS             26002..26478
                     /locus_tag="VUW49_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET7981100.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26506..27411
                     /locus_tag="VUW49_001178"
     CDS             26506..27411
                     /locus_tag="VUW49_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET7981101.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27398..28213
                     /locus_tag="VUW49_001179"
     CDS             27398..28213
                     /locus_tag="VUW49_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET7981102.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28220..28624
                     /locus_tag="VUW49_001180"
     CDS             28220..28624
                     /locus_tag="VUW49_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7981103.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            28923..30083
                     /locus_tag="VUW49_001181"
     CDS             28923..30083
                     /locus_tag="VUW49_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7981104.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATILVSFARSGNSPESVATVDLAKSLVDELYQVTITCATDGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEY"
     gene            30205..31242
                     /locus_tag="VUW49_001182"
     CDS             30205..31242
                     /locus_tag="VUW49_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET7981105.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
BASE COUNT         8562 a         6198 c         7368 g         9206 t
ORIGIN      
        1 ttttttatcc aaaaagtgca gttgggaggg agataggctc atttgggaag gaagtccagt
       61 ttttgtttag tgattggggt aagatagttg ttatcagatg agtttatact cttcgaaaat
      121 caaattcaaa ccacgtcaac gtcgccttgc cgtatatatg tgactgactt cgtcagtcct
      181 atctacaacc tcaaaacagt gttttgagca gcctgcggct agtttcctag tttgctcttt
      241 gattttcatt gagtattagg gaaaaggaga tgaatatgaa atttgggaaa cgtcactatc
      301 gtccgcaagt ggatcagatg gactgcggtg tagcttcatt agccatggtt tttggctact
      361 atggtagtta ttattttttg gctcacttgc gagaattggc taagacgacc atggatggga
      421 cgacggcttt gggcttggtc aaggtggcag aggagattgg ttttgagacg cgagccatta
      481 aggcggatat gacgcttttt gacttgccgg atttgacttt tccttttgtt gcccatgtgc
      541 ttaaggaagg gaaattgctc cactactatg tggtgactgg gcaggataag gatagcattc
      601 atattgccga tccagatccc ggggtgaagt tgactaaact gccacgtgag cgttttgagg
      661 aagaatggac aggagtgact ctttttatgg cacctagtcc agactataag ccttataagg
      721 aacaaaaaaa tggtctgctc tcttttatcc ctatattagt gaagcagcgt ggcttgattg
      781 ccaatatcgt tttggcaaca ctcttggtaa ccgggattaa cattgtgggt tcttattatc
      841 tgcagtctat cattgatacc tatgtgccag atcagatgcg ttcgacacta gggattattt
      901 ctattgggct agtcatcgtc tacatcctcc agcaaatctt gtcttacgct caggagtatc
      961 tcttacttgt tttggggcaa cgcttgtcga ttgacgtgat tttgtcctat atcaagcatg
     1021 tttttcacct ccctatgtcc ttctttgcga cacgcaggac aggggagatc gtgtctcgtt
     1081 ttacagatgc taacagtatc atcgatgcgc tggcttcgac catcctttcg attttcctag
     1141 atgtgtcaac ggttgtcatt atttcccttg ttctattttc acaaaatacc aatctctttt
     1201 tcatgacttt attggcgctt cctatctaca cagtgattat ctttgccttt atgaagccgt
     1261 ttgaaaagat gaatcgggat accatggaag ccaatgcggt tctgtcttct tctatcattg
     1321 aggacatcaa cggtattgag actatcaagt ccttgaccag tgaaagtcag cgttaccaaa
     1381 aaattgacaa ggaatttgtg gattatctga agaaatcctt tacctatagt cgagcagaga
     1441 gtcagcaaaa ggctctgaaa aaggttgccc atctcttgct taatgtcggc attctctgga
     1501 tgggggctgt tctggtcatg gatggcaaga tgagtttggg gcagttgatt acctataata
     1561 ccttgctggt ttactttacc aatcctttgg aaaatatcat caatctgcaa accaagcttc
     1621 agacagcgca ggttgccaat aaccgtctaa atgaagtgta tctagtagct tctgagtttg
     1681 aggagaagaa aacagttgag gatttgagct tgatgaaggg agagatgacc ttcaagcagg
     1741 ttcattacaa gtatggctat ggtcgagacg tcttgtcgga tatcaattta accgttcccc
     1801 aagggtctaa ggtggctttt gtggggattt cagggtcagg taagacgact ttggccaaga
     1861 tgatggttaa tttttacgac ccaagtcaag gggagattag tctgggtggt gtcaatctca
     1921 atcagattga taaaaaagcc ctgcgccagt acatcaacta tctgcctcaa cagccctatg
     1981 tctttaacgg aacgattttg gagaatcttc ttttgggagc caaggagggg acgacacagg
     2041 aagatatctt acgggcggtc gaattggcag agattcgaga ggatatcgag cgcatgccac
     2101 tgaattatca gacagaattg acttcggatg gggcagggat ttcaggtggt caacgtcaga
     2161 gaatcgcttt ggcgcgtgct ctcttgacag atgcgccggt cttgattttg gatgaggcga
     2221 ctagcagttt ggatattttg acagagaagc ggattgtcga taatctcatt gctttggaca
     2281 agaccttgat tttcattgct caccgcttga ctattgctga gcggacagag aaggtggttg
     2341 tcttggatca gggcaagatt gtcgaagaag gaaagcatgc tgatttgctt gcacagggag
     2401 gcttttacgc ccatttggtc aatagctaga aagaggagag gatgaaacca gaatttttag
     2461 aaagtgcgga gttttataat cgtcgttacc ataatttttc cagtagtgtg attgtaccca
     2521 tggcccttct gctcgtgttt ttacttggct ttgcaactgt tgcagagaag gagatgagtt
     2581 tgtccactag agctactgtc gaacccagtc gtatccttgc aaatatccag tcaactagca
     2641 acaatcgtat tcttgtcaat catttggaag aaaataagct ggttaagaag ggggatcttt
     2701 tggttcaata ccaagaaggg gcagagggtg tccaagcgga gtcctatgcc agtcagttgg
     2761 acatgctaaa ggatcaaaaa aagcaattgg agtatctgca aaagagcctg caagaagggg
     2821 agaaccactt tccagaggag gataagtttg gctaccaagc cacctttcgc gactacatca
     2881 gtcaagcagg cagtcttagg gctagtacat cgcaacaaaa tgagaccatc gcgtcccaga
     2941 atgcagcagc tagccaaacc caagccgaaa tcggcaacct catcagtcaa acagaggcta
     3001 aaattcgcga ttaccagaca gctaagtcag ctattgaaac aggtacttcc ttggccggtc
     3061 agaatctagc ctactctctt taccagtcct acaagtctca gggcgaggaa aatccccaaa
     3121 ctaaggttca ggcagttgca caggttgaag cacagatttc tcagttagaa tctagtcttg
     3181 ctacttaccg tgtccagtat gcaggttcag gtacccagca agcctatgcg tcagggttaa
     3241 gcagtcaatt ggaatccctt aaatcccaac acttggcaaa ggttggtcag gaattgaccc
     3301 ttctagccca gaaaatcttg gaggcagagt caggtaagaa ggtacaggga aatcttttag
     3361 acaaggggaa aattacggca agtgaggatg gggtgcttca tcttaatcct gagaccagtg
     3421 attctagcat ggttgcagaa ggtgccctac tagcccaact ttatccatct ttggaaagag
     3481 aagggaaagc caaactcaca gcctatctaa gttcaaaaga tgtagcaaga atcaaggtcg
     3541 gtgattctgt tcgctatact acgactcatg atgccgggaa tcaacttttt ctagattcta
     3601 ctattacaag tattgatgcg acagctacta agactgagaa agggaatttc tttaaaatcg
     3661 aggcggagac taatctaact tcggagcagg ctgaaaaact taggtacggg gtggaaggcc
     3721 gtctacagat gattacgggc aagaaaagtt atctacgtta ttatttggat caatttttga
     3781 acaaagagta atgttcgtgt ttttagagtt aaataatttt taaactgtga gaaagattct
     3841 tcttgcagtt ttttctttac aatttttgaa aaacatctgc tatttattcg gttaaattct
     3901 tgtgtttttt ggttttttgt ggtaaaatgt gctcaagtaa tacgaaaggc gaactttaaa
     3961 atgtcaaaac aattgatcta ttcgggaaaa gctaaagata tctatacaac tgaggatgaa
     4021 aatcttatta tttcaactta caaggaccag gcgactgctt tcaatggtgt caagaaggag
     4081 cagattgcag gtaagggagt cttgaataat cagatttcat cttttatttt tgagaaatta
     4141 aatacggctg gtgtggcgac tcactttgtg gagaaacttt cagacacgga acaactcaat
     4201 aaaaaggtta agattattcc tttggaagtc gtgctccgca actatactgc tggttccttt
     4261 tcaaaacgtt ttggtgtgga tgagggaatc gccttggaga ctccgattgt cgaattttac
     4321 tacaaaaatg atgatttgga tgatccattt atcaatgatg agcatgtgaa attcctacag
     4381 attgcgggtg accagcagat tgcctacttg aaggaagaaa cgcgtcgtat caatgaacta
     4441 ttgaaagtct ggtttgctga gattgggctt aaattgattg actttaagct agagttcggt
     4501 tttgacaagg atggcaagat tatcttggca gacgaatttt caccagataa ctgccgcttg
     4561 tgggacgctg atggcaacca catggataag gatgttttcc gtagaggatt gggagaacta
     4621 accgacgttt atgagattgt ttgggaaaag ttgcaggaat tgaaataatc tgtttgcaac
     4681 ggaaaacctt cgtctctcaa ctaaaaggac tcaggctgaa aaggtccccc agaccttttc
     4741 actctgtaga gaactaggtg aactaacaga tgtttacgaa attgtctggg aaaagttgca
     4801 gggtttaaaa taacaacctc aaggctgttt gggaatattg caagagctga aataaaggaa
     4861 taagaattga tggataaacg tatttttgtt gaaaaaaagg ctgattttca ggtcaagtca
     4921 gagagtttgg ttagagagct ccagcacaac ttgggactgt caagcttgaa aagtattcgt
     4981 attgtgcaag tatatgatgt atttgacttg gctgaggact tgtttgcacc tgcagagaag
     5041 cacattttct ctgagcaggg aaccgaccat gttttagatg aagtatctgt gcaggcggat
     5101 cttgctaact atgctttctt tgccattgaa agtctgccag ggcagtttga ccagcgtgca
     5161 gcttcgtcac aggaagcctt gcttttgttg ggaagttcga gtgacgtgac agtcaacaca
     5221 gcccaacttt acttggtgaa taaagatatt gatgcgactg agttggaagc tgtcaaaaac
     5281 tacctgctca atccagttga ttctcgtttc aaggatatca cgacagggat tgccaagcag
     5341 gagttttcag agtcagacaa gaccattcca aaattgactt tctttgaaag ctatacagca
     5401 gaagactttg ctcgctacaa ggccgagcaa gggatggcca tggaagtgga tgatttgctc
     5461 tttatccaag actactttaa gtcaatcggg cgcgtgccaa ctgagactga actcaaggtt
     5521 ttggacactt actggtctga ccactgccgt catacgactt ttgagacaga gttgaaacac
     5581 atcgactttt cagcttctaa atttcaaaag caattgcagt caacctatga caagtatatt
     5641 gccatgcgcg aggagttagg tcggtctgaa aaaccacaaa ccttgatgga tatggcgact
     5701 attttcggtc gttatgagcg tgctaatgga cgattggatg atatggaagt ctctgacgaa
     5761 atcaatgcct gctcagttga aattgaagtg gatgttgatg gtgtcaagga accttggctc
     5821 ctcatgttta aaaatgaaac ccacaaccat ccaacagaaa ttgagccatt tggtggagcg
     5881 gctacctgta tcggtggagc tattcgtgat ccgttgtcag gccgttccta tgtttaccaa
     5941 gccatgcgta tttcaggtgc tggtgatatt acagcaccga tttcggaaac tcgtgctggg
     6001 aaattgccac aacaagtcat ttctaaaaca gcagctcatg gttattcttc atatggtaac
     6061 cagattgggc ttgccacaac ctacgttcgt gaatacttcc acccaggctt tgtagctaaa
     6121 cgtatggaac ttggtgccgt tgttggtgcg gctcccaagg gcaatgttgt ccgtgaaaaa
     6181 cctgaagcag gtgatgtgat catccttctc ggaggcaaaa caggtcgtga tggtgtcggt
     6241 ggtgcgacgg gctcttctaa ggttcaaaca gttgagtctg tagagactgc tggtgctgag
     6301 gttcaaaaag gaaatgccat cgaagaacgc aagattcagc gcctcttccg taatggcaat
     6361 gtcactcgtc tgatcaagaa gtccaatgac tttggggcag gcggcgtctg tgtggctatc
     6421 ggtgaattgg cagacggtct tgaaatcgac ctcaacaagg tgcctcttaa ataccagggc
     6481 ttgaatggta cagaaattgc catctctgaa tcacaagaac ggatggcggt cgtggttcgt
     6541 cctgaagatg tggatgcctt cgttgccgaa tgtaacaaag aaaatattga tgctgttgtg
     6601 gtggcgacag taactgaaaa accaaatctt gtcatgcact ggaatggtga gacaatcgtt
     6661 gacttggagc gtcgtttcct tgacaccaat ggtgtgcgcg tggttgtcga tgccaaagtt
     6721 gtggacaagg atgtcaaact cccagaagag cgtcaaacat ctgctgaaac actggaatca
     6781 gataccctta cggttctatc tgacctcaac catgcaagtc aaaaaggatt acagactatc
     6841 tttgactgct ctgttggacg ctcaacggtt aatcacccac ttggtggtcg ttaccaactc
     6901 acaccaactg aggcatctgt gcagaaattg ccagttcaac acggtgtgac tcatactgcg
     6961 tcggtcattg ctcaaggttt caacccatat gtagctgaat ggtctccata ccacggtgct
     7021 gcttatgcgg ttatcgaagc aactgctcgt ttggtggctg ctggtgccaa ctggtccaag
     7081 gctcgtttct cttaccaaga gtatttcgag cgtatggata aacaagcaga gcgtttcggt
     7141 cagccagtag ctgctcttct aggttctatt gaagcacaaa ttcagcttgg cttgccatct
     7201 atcggtggta aggactccat gtctggtacc tttgaagaat tgaccgttcc gccaaccttg
     7261 gttgcctttg gggtgacgac ggcagatagc cgtaaggtgc tctctccaga atttaaagct
     7321 gttggggaaa atatctacta catcccaggt caagccctct ctgcagagat tgattttgac
     7381 ttgattaaga aaaattttgc tcagtttgaa gcaagccaag ctgaccataa agtgacatct
     7441 gcatcagctg tcaaatacgg tggtgtagtt gaaagtttgg ctcttgctac ctttggaaac
     7501 tatattggtg cagaggtgac cttgcctgaa cttaaaacag ctttgacagc tcaattaggc
     7561 ggctttgtct tcacatctcc tgaagaaatt gctggagtag agaagattgg acaaacgaaa
     7621 gcagacttta cactgactgt caacggtgtg aagctagatg gacacaagct tgacagtgca
     7681 tttcaaggga cattggaaga agtttaccca acagaattta cccaagcgaa agaactagaa
     7741 gaagtaccag ctgtggcatc agatgttgtg attaaagcca aagaaaaggt tgaaaaacct
     7801 gtggtttaca tcccagtctt tccaggaacc aactcagaat atgattcagc taaggccttc
     7861 gaaaaagaag gtgcagaggt caatttggtg ccattcgtga ccttgaatga agaagctatt
     7921 gtcaagtcag ttgaaactat ggttgacaat atcgacaaga ctaatattct cttctttgcc
     7981 ggtggattct cggctgcgga tgaaccagat ggttcagcta agtttatcgt caatatcctg
     8041 cttaatgaaa aagtgcgtgt ggctattgat agctttatcg cccgtggtgg cttgattatc
     8101 ggtatttgta atggattcca agccttagtc aaatcgggtc tcctaccata cggaaacttt
     8161 gaagatgcta acagtactag tccaaccctc ttctacaatg atgccaacca acacgtggcc
     8221 aagatggtgg aaactcgcat tgccaatacc aactcaccat ggttggctgg tgtgcaagtg
     8281 ggcgatatcc acgctattcc tgtttcgcac ggtgaaggga agtttgtcgt gacggctgag
     8341 gaatttgcag agctccgtga caatggacaa attttcagcc aatacgttga ctttaacggt
     8401 aaaccaagta tggattctaa gtacaatccg aatggttctg tccatgccat cgaaggaatt
     8461 accagcaaga atggtcaaat catcggtaag atgggccact cagaacgtta tgaggatggt
     8521 cttttccaaa atatcccagg caataaagac caacacctgt tcgcatcagc ggttaaacat
     8581 ttcactggaa aataagactt acagattttc taatagatag tatcagtaat gtaaaagtca
     8641 tgtaaatcta gctcttgatg attacaaatg aaaattaggt ataaaaaatg acatacgaag
     8701 taaaatctct taatgaagaa tgtggtgttt tcggtatttg gggacatcca gatgctgcta
     8761 agttgaccta ttttggactc cacagtcttc aacaccgtgg tcaggagggg gcaggaatcc
     8821 tctccaacga tcaaggacaa ctgaagcgcc atcgtgacat ggggctttta tcagaagttt
     8881 tcagaaatcc agctaatttg gataaattga caggagctgg tgcgattggg catgtgcgtt
     8941 atgcgactgc tggcgaagct tctgtagata acatccagcc cttcctcttc cgttttcacg
     9001 atatgcagtt tggcttggct cataatggaa atctgaccaa tgcagcctct ctcaagaaag
     9061 aactggaaca aagaggagca attttcagcg cgacttcgga ctctgaaatc ttggctcacc
     9121 tcattcgtcg cagtcataat cctagcctga tgggcaaaat caaggaagcg ctcagccttg
     9181 tcaaaggtgg ttttgcctat atcttgctgt ttgaggacaa gttgattgcg gctcttgacc
     9241 ctaatggctt tcgtccgctt tctatcggga aaatggccaa cggagcggtg gttgtttcct
     9301 ctgaaacctg tgcttttgag gtcattggtg ccgagtggat tcgtgatttg aagccaggtg
     9361 agattgtgat cattgatgac gagggcattc agtatgacag ctatacagat gatacccagt
     9421 tggcgatttg ttctatggag tatatctact ttgctcgccc tgattctaat atccacggtg
     9481 tcaatgtcca tacggcacgt aagagaatgg gagcgcaatt ggcgcgagaa tttaagcatg
     9541 aggcagatat tgtagttggt gtgcccaatt cttccctaag cgcggctatg ggatttgcgg
     9601 aagaatcagg cttaccaaat gaaatgggtc tgatcaaaaa ccaatacacc cagcgaactt
     9661 ttatccaacc gactcaagaa ttgcgggagc aaggagtgcg gatgaaactg tctgctgttt
     9721 cgggtgttgt caaaggcaaa cgtgtggtca tggtggatga ttccattgta cgtggaacaa
     9781 cctctcgtcg tatcgttcag ctcttgaaag aagcgggtgc gactgaggtt cacgttgcca
     9841 ttggaagtcc tgcactagcg tatccatgtt tctacgggat tgatatccag acccgtcagg
     9901 agctgattgc agccaatcat acggtcgaag aaactcgcca aatcattggt gcggacagtc
     9961 tgacttatct ttcaattgat agcttgattg agtcgattgg tatcgaaaca gatgcgccga
    10021 acggtggtct ctgtgtcgct tactttgacg gtgactaccc aacgcctctc tatgactacg
    10081 aagaagacta tcgtagaagt ttggaagaaa agaccagttt ttacaagtag gcgacagatt
    10141 ctccattaaa gaaaaggaaa aataaaatgg caaataaaaa tgcgtacgct caatctggtg
    10201 tggatgttga agcgggttat gaagttgttg aacggattaa aaagcacgtg gcccgtacgg
    10261 agcgtgcagg tgtcatggga gctcttggtg gctttggtgg tatgtttgac ctttccaaga
    10321 ctggggttaa agaacccgtc ttgatttcag ggactgacgg tgtcggaacc aagctcatgt
    10381 tggctatcaa gtacgacaag cacgatacca tcgggcagga ctgtgtggcc atgtgtgtca
    10441 acgacatcat tgctgcaggt gcggaacccc tctattttct cgactacgta gcgacaggga
    10501 agaatgaacc agctaagcta gaacaagtgg ttgctggtgt ggcagaaggt tgtgtgcagg
    10561 ctggtgctgc cctcatcggt ggggaaacgg ctgaaatgcc gggcatgtac ggcgaagacg
    10621 actatgactt ggctggtttt gcggtcggtg tggctgaaaa atctcaaatc attgacggtt
    10681 caaaggtggt agagggagat gttcttctcg gacttgcttc aagtgggatt cactcaaatg
    10741 gttactcttt ggttcgtcgt gtctttgcgg attacacagg tgaggaagtc ctaccagaat
    10801 tggaaggcaa gaaacttaag gaagttctac ttgagccgac tcgtatctat gtcaaggctg
    10861 tcttgccgct catcaaagaa gagttggtca acggcattgc ccacatcaca ggtggtggct
    10921 ttatcgaaaa tgtccctcgt atgtttgcag atgacctagc tgctgaaatt gatgaaagca
    10981 aagttccagt gcttccaatt ttcaaagccc ttgaaaaata tggtcagatt aaacacgaag
    11041 aaatgtttga aatcttcaat atgggtgtgg gacttatgtt ggcggtcagc cctgaaaatg
    11101 tagagcgtgt aaaagaattg ttggatgaag cagtctatga aattggtcgc atcgtcaaga
    11161 aagaaaacga aagtgtcatt atcaaatgaa aaaaatagcg gtttttgcct ctggtaatgg
    11221 ctcaaatttt caggtgattg ccgaagaatt tccagtggag tttgtctttt cagaccatcg
    11281 tgatgcctat gtgcttgagc gtgcaaagca gctcggcgtt ctgtcctatg cttttgaact
    11341 caaggagttt gagagcaaga cagactacga agcagccctt gtcgaactct tggaagaaca
    11401 ccagattgac ttggtttgcc tagcaggcta catgaaaatc gttggaccaa ccttattgtc
    11461 ggcttatgaa ggtcggattg tcaacattca tccagcctac ttgccagaat ttccaggagc
    11521 tcatgggatt gaggatgctt ggaatgctgg cgtgggtcag tctggtgtga ccattcactg
    11581 ggtggattcg ggtgtggata caggccatgt catcaaacag gttcgtgtgc cacgactagc
    11641 tgatgatacc attgacagat ttgaagctcg catccatgaa gcagagtaca ggttgtatcc
    11701 ggaagtagtg aaggctctat ttacagattg actttttgat gattcatatg atatctttga
    11761 ttttaaattg gagtcagtgt ttgttgaaga cggcttcaaa cggaggtatt tgtaatgtta
    11821 gaatctaaaa aaacaactcg atatgtattt tatgtctatc tgatgttatt aacttgggga
    11881 atcttattta agtttgaaac aaatcctgaa tttatagcat ttttcttagc tccaaggtat
    11941 atcaattgga ttccattttc agaaccacta atagtcgatg gaaaaattgt ttttgctgaa
    12001 atgttattta atctgatttc ctttattcca ttaggtgttt gtttcccttt gataaaaact
    12061 aatttatcta gtttaagaat agtcgggaca ggtttcttga ttagtttatt gtttgagtgc
    12121 ttacagtata ttttagcaat aggtataaca gatataacgg atttgacttt aaatacgcta
    12181 ggtgtctgtg taggcttact gatttatcaa atttttataa gagtgttcaa atcacagact
    12241 agaaaatgga tcaatatctt aggtatgctt agccttggtt ttgcttatct tgttttactg
    12301 ttactgcatt taattggtgt ttaactaatg attaaaaagg agaatataat gactaaacgc
    12361 gtcttaatca gcgtctcaga caaagcgggc attgttgaat ttgcccaaga actcaaaaaa
    12421 cttggttggg agattatctc aacaggtgga actaaggttg cccttgataa tgctggggtg
    12481 gataccattg ctatcgatga tgtgactggt ttcccagaaa tgatggacgg tcgtgtgaag
    12541 accctccacc caaatatcca cggagggctt ctcgctcgtc gtgacttgga tagccacttg
    12601 gaagcggcta aggacaacaa gattgagctc attgaccttg tggtggtcaa cctttaccca
    12661 tttaaggaaa ctatccttaa accagatgtg acttatgctg atgcagttga aaatatcgat
    12721 attggtgggc catctatgct tcgttcagca gcgaaaaatc atgccagtgt tacagttgtg
    12781 gtagatcctg ctgactacgc tgtggttttg gacgaattgg cagcaaacgg cgaaacctct
    12841 tatgaaactc gccaacgttt agcagccaaa gtatttcgtc acacagcggc ttatgacgcc
    12901 ttgattgcag aatacttcac agctcaagtg ggtgaaagca agcctgaaaa actcactttg
    12961 acttatgacc tcaagcaacc aatgcgttac ggtgagaatc ctcaacaaga cgcggacttt
    13021 taccagaaag ctttgcctac agattactct attgcttcag ccaaacagct caacgggaaa
    13081 gaattgtcat ttaataatat ccgtgatgca gatgctgcta tccgtatcat ccgtgacttc
    13141 aaagatagtc caaccgttgt ggctctcaaa cacatgaatc catgtggaat tggtcaagct
    13201 gatgacatcg agactgcttg ggactacgct tatgagtctg atccagtatc tatctttggt
    13261 gggattgtcg tcctcaaccg tgaggtggat gctgcgacag ctgagaagat gcacggcgtt
    13321 ttcctcgaaa tcatcattgc accaagctat acggatgaag cgctagctat tttgatcaat
    13381 aaaaagaaaa acttgcgtat ccttgccttg ccatttaatg ctcaagaggc tagcgaagtg
    13441 gaagcagaat acacaggtgt agtcggtgga cttctcgtgc aaaatcaaga cgtggtcaag
    13501 gaaagcccag ctgactggca agtggtgacc aaacgtcagc caaccgagac agaagcgact
    13561 gctcttgagt tcgcttggaa ggctatcaag tacgtcaaat caaatggtat tatcgtgacc
    13621 aacgaccaca tgacacttgg tgttggtcca ggtcaaacca accgtgtggc ttctgttcgc
    13681 cttgccattg accaagccaa agatcgtctg gacggggcgg tccttgcttc agatgccttc
    13741 ttcccatttg cggataacgt ggaagaaatc gccaaagcag gaattaaggc catcatccag
    13801 cccggtggct ctgtccgtga ccaagaatct atcgaagcgg cagacaaata tggcttgacc
    13861 atggtcttta caggcgtgag acattttaga cattaagaag ataaaaggga agaaaacagt
    13921 ttctttcctt ttttggctta aaatgctaac tgaaacaaga ttaaaacgaa cttttttgat
    13981 ataatgttgg taaataattc gcaaaagagg ttgaggaatg aaactgcttg ttgtcggttc
    14041 tggtggtcgt gagcatgcga ttgctaaaaa gttacttgaa tcaaaagacg tggaaaaagt
    14101 ctttgtagct cctgggaatg atgggatgac tctggatggt ttggaattgg taaatatctc
    14161 tatttccgaa cattctaaat tgattgactt cgcaaagacc aatgatgttg cttggacctt
    14221 tatcggtcca gatgatgccc ttgctgctgg tatcgtggat gattttaacc aagctggact
    14281 taaggccttt ggtccgacta gggctgcagc ggagctggag tggtccaagg atttcgccaa
    14341 ggaaatcatg gtcaaatacg gcgttccgac agcaacatat ggcacatttt cagatttcga
    14401 ggaagccaag gcctatatcg aaaagcatgg tgcgcctatc gtagtcaagg cggatggctt
    14461 ggcacttggg aagggtgtcg tcgttgctga tacggttgag caagcggtcg aagccgctca
    14521 tgagatgctt ttggacaata aatttggtga ctcaggtgcg cgcgtggtta ttgaggaatt
    14581 ccttgaagga gaggaatttt cactctttgc ctttgtcaat ggtgataagt tctacatcat
    14641 gccaacggct caggaccaca aacgtgccta tgatggcgac aaagggccta acacgggtgg
    14701 tatgggtgcc tatgcgccag tcccacactt accacagagt gtagttgata cagcggttga
    14761 caccattgtc aagccagttc tagaaggggt gattaaagaa ggtcgccctt atctgggagt
    14821 tctttacgca gggcttatcc tgacagctga tggaccgaaa gtcattgagt tcaacgctcg
    14881 gttcggagat ccagaaactc agattatctt gcctcgcttg acctctgact ttgctcaaaa
    14941 tatcacagat atcctggata gcaaggagcc aaatatcatg tggacggaca agggtgtgac
    15001 tctgggtgtg gttgtcgcat ccaagggcta cccgctagac tatgaaaggg gcgttgagtt
    15061 gccagccaag acagaaggcg atgtcatcac ctactatgca ggggctaagt ttgcggaaaa
    15121 tagcagagca ctgctctcaa acggcggacg agtttatatg ctcgttacca cagcagatac
    15181 cgtcaaagaa gcccaagcca gcatatacca agaactatac caacaaaaaa tagaaggact
    15241 cttctaccga acagatatcg gaagcaaggc aattaagtaa agatataaga ataacgcgcc
    15301 gtagtcgcca aacacgataa tggtcgtcgt ggtgaaaaga ccagaacagt gaatgttctg
    15361 gtcaggggga aacttggaga ccgtaggctc aaagtttagg aatgaaaccg aaggtttgct
    15421 tccgcctcca tcacctaaga ccattatcaa aaagaaaaat aaaaattcac aaaatacgtt
    15481 aatgatcgta tggtttgcga gcgttagcga gctaatatag aacaatcacc gccgttgtga
    15541 aagaacgatt ggatgataat ccaatcgttc agggaaattg gaagaccttg ggtttccaat
    15601 ttaggcatga gacacctttg gtggctgctg ccgtccctca caagctaagg tgattgttga
    15661 aaaagaggaa aaaggagaag aaatgaaacc agtaatttcc atcatcatgg gctcaaaatc
    15721 cgactgggca accatgcaaa aaacagcaga agtcctagac cgcttcggtg tagcatacga
    15781 aaagaaagtt gtttccgcac accgtacacc agacctcatg ttcaaacatg cagaagaagc
    15841 ccgtagtcgt ggcatcaaga tcatcatcgc aggtgctggt ggcgcagcgc atttgccagg
    15901 catggtagct gccaaaacaa cccttccagt cattggtgtg ccagtcaagt ctcgtgctct
    15961 tagtggagtg gattcactct attctatcgt tcagatgccg ggtggggtgc ctgttgcgac
    16021 catggctatc ggtgaagctg gagcgactaa cgcagctctc tttgccctcc gtctcctctc
    16081 tgtagaagat aagtccattg cggatgcact tgccaacttt gctgaagaac aaggaaaaat
    16141 cgcagaggag tcgtcaaatg agctcatcta aaacaatcgg aattatcggt ggcggtcaac
    16201 tgggtcagat gatggccatt tctgctatct acatgggcca caaggttatc gcgctggatc
    16261 ctgcggcgga ttgcccggcc tctcgcgtgg cggaaatcat tgtggcacct tataacgatg
    16321 tggacgccct ccgtcagttg gcagaccgtt gcgatgtcct cacttacgag tttgaaaatg
    16381 tcgacgctga cggtttggat gccgttatca aggatggaca actccctcaa ggaacagatc
    16441 tgctccgcat ttcgcaaaat cgtatttttg aaaaggactt tttgtcaaac aaggctcaag
    16501 tcactgtggc accctacaag gtcgtgactt ctagcctaga cttggcagat atcgacttgt
    16561 cgaaaaacta tgtcctcaag actgcgactg gtggctacga tggtcatgga caaaaggtta
    16621 ttcgttcaga agtagacttg gaagcagcct atgcgctagc agactcagca gactgcgtct
    16681 tggaagaatt tgtcaacttt gaccttgaga tttctgtcat cgtgtcagga aatggcaagg
    16741 aggtgacgtt tttcccagtt caggaaaata tccaccgcaa caatatcctg tctaagacca
    16801 tcgtaccagc ccgcatttct gaaagtctag tagacaaggc taaagctatg gcagtgcgaa
    16861 tcgcagaaca actcaacttg tctggaactc tctgtgtgga aatgtttgcg actgatgatg
    16921 acgttattgt caatgaaatc gccccacgac cacataactc tgggcactat tctattgaag
    16981 cctgtgattt ctctcagttt gacacccata ttctcggtgt tctcggagca ccattaccag
    17041 tcatcaaact ccatgcgcca gccgttatgc tcaatgtcct aggtcaacac gtcgaggctg
    17101 ctgaaaagta tgtcgcagaa aatccaagcg cccacctcca catgtatggt aaaatagaag
    17161 caaagcataa tcgtaagatg ggacatgtga ccttgtttag tgatgtaccg gatagtgtgg
    17221 atgagcttgg ggaagggatt gatttttagg acaagtctat gatacaaatt atcgttaata
    17281 catttattga aaaggataag actggagcag ttgttgaagt gttgtatgcc agtgctgacc
    17341 aagataaggt acaagctaaa tatgaagaac tagctgcaca ataccccgaa aattatttag
    17401 ctatctatga tgtaccgctg gatacggatt tgaatacact agatcattac ccgtctgtgt
    17461 ttattggaaa agaggagttt gagtagaaat cttggtttac ctagatagct tattcccaac
    17521 agcttaagaa gaaaggaaaa attaacacat gatcaaccgt tactctcgcc ctgagatggc
    17581 gaatatttgg agtgaagaaa ataaataccg tgcttggctt gaggtggaaa tcctctctga
    17641 cgaggcatgg gctgagttgg gggaaatccc taaggaagat gtggctttga ttcgcaagaa
    17701 ggcggacttt gacatcgacc gtattttgga aattgagcag gagacgcgcc acgatgtggt
    17761 ggctttcacg cgtgcggttt ctgagactct tggtgaagag cgcaagtggg ttcactatgg
    17821 gttaacttct actgacgtgg tggatactgc ttatggttac ctctacaagc aggccaacga
    17881 catcatccgt cgtgaccttg aaaacttcac taatatcatc gctgacaagg ccaaggagca
    17941 caagttcacc atcatgatgg ggcgtactca tggtgtgcac gctgagccga caacctttgg
    18001 tcttaaatta gcaacttggt acagcgaaat gaaacgcaat atcgagcgct tcgagcatgc
    18061 ggctgctggt gtagaagctg gtaagatttc tggtgcggtt gggaactttg ccaatatccc
    18121 accatttgta gaggagtatg tctgcgataa acttggcatc cgtgcccaag aaatctctac
    18181 acaagttctt cctcgtgacc ttcacgctga gtactttgcg gttcttgcca gcattgcgac
    18241 ttcaatcgaa cgtatggcga ctgagattcg tggtctacaa aaatctgagc aacgcgaagt
    18301 agaagagttc tttgctaaag ggcaaaaagg gtcttcagca atgcctcaca aacgcaaccc
    18361 aatcggttct gaaaatatga ctggtctggc gcgtgtcatt cgtggtcaca tgattacggc
    18421 ttatgaaaac gtcgctctct ggcacgaacg cgatatttct cactcatcag ctgagcgtat
    18481 catcacacca gatacgacca ttttgattga ctacatgctc aaccgttttg gaaatatcgt
    18541 caagaacttg acagtcttcc cagaaaatat gatccgaaac atgaactcga cttttggtct
    18601 tatctttagc caacgggcta tgttgacatt gattgaaaaa ggcatgaccc gtgagcaagc
    18661 ctatgacttg gtgcaaccaa aaacagccta ctcttgggac aaccaagtag actttaaacc
    18721 acttcttgag gcagattcag aagtaacatc acgtctcaca caagaagaaa tcgatgaaat
    18781 cttcaaccca gtttattaca ccaaacgagt ggatgatatc tttgaacgtc ttggactagg
    18841 tgattaatta aaaaataaac agcgagcttc aatctcgctg tttatttttt atcgaaaaga
    18901 cttagtcttc ttttctttta gtgagtccat aggctgctag tgtggccatg agtcctgcga
    18961 ctactagtcc tgcagaatcg tgagttcctg tttcaggaag ttttttctct attaccacag
    19021 gagctggatc ttgaggaaga gctttgcttt cctcagcagg agcagttgat ggagctggtt
    19081 ggcttgggat ttctagtttt ggtttttctt cagcaatagc ggcttgtccg ttttcatcgc
    19141 ctacatgtgt taccatagtt ccgacttcga ctatttgagt aacggcttcc tgtgctacga
    19201 cactatttac aagtgttttc acttccttac catcggcaga agtgctcaca gagtagaagt
    19261 tgctacgatg tccattgacg cccttagtaa tgacttgtgt ttttcctttg agtaagagtg
    19321 gattttcacg agtcactgtg gtaaatggaa tttcttcttt ttggatatcc agtctaggtt
    19381 ttacctcagt agttggtgca agaccacttt catcaccctt gtgagttaca ggagcgccaa
    19441 cttcaaccac ttggtttata acttctttgg ttacctggct atcaaggact gtttctgttg
    19501 tttttccatt ttcagtgagt acagagatgt aatgagttcg ctcacctttg actcctgctg
    19561 tgataatatt ttcctgacca gctgggaggt taggattttc tttcttgata acttcaaatg
    19621 gaatttcttc agttcttgtg atgagttctg gtctggtttc aacattggca gccacttcat
    19681 tttcatccag gcttcctgaa tgagttgcag ctggtttgag gccttgaaga gcggctttta
    19741 ggttggctac aagtgtgtca agctcagctt gttgattacg gttgaggttg taatttagag
    19801 ctgttttagc tgcgtcaagg gcctcaagac tttctttact atatccttct aagtttgtag
    19861 gaattttagc taattcttcg cggagagcat tataattagc acggaagtag tctttgttgt
    19921 ggtctgcaaa ggcagtcatg agttcaaaga tttcctcttc cttgtattca gcgcttggtc
    19981 tatctgccca gattgaaagc atacttccga ctgttggaag atctacttca ggatatttgg
    20041 tagaagctag ttgattgaat ggtgtttttc cagtattctc aatagctttc ttgaggaaac
    20101 caccaccatc ttctggtttt tgaccaagaa tgtagtacca gtcaccgttg gtattcaaga
    20161 atttatagcc tttgcttgct aggtattgag gtgatgcgag gttatatccc caccagcctt
    20221 tagaccagta agaaatcaag acatctttgt caaactgaac atcgtccttg tcttcatagt
    20281 agaagccatc gttgaaggcc attggttgaa gctctctttc tttggccata gctgcgaggg
    20341 tgttggcata ttcggcaaat ttgccataga gttgatacca cttgaggtag taccagcctt
    20401 gggcactagt cgcatcgttg gcgtattcgt cagtaccaaa gttgaaaatc tttgttttac
    20461 ctgcaaagaa gtccatgtat ttaccgatga gggcttttac aaagttcatc gcttcttcgt
    20521 ttttcaagtc catagttgtt tttgaaactt tatcaaagtg ggcttgagga tttttaatac
    20581 ctaatttttc catggcaacc agcatagcat ccatgtgacc tggactgtta atagctggga
    20641 tgagaccgat gtccttagat ttagcgtatt caattagctc tgtcacttct gcctgtgtta
    20701 gtgcagtacc gtttggatcg tcgtagtaag ctttagttcc ttcgataata gcttttttaa
    20761 cgtcatcact agcataggtt tttccgttgg cagtaatggt catatcattg agtagaaagc
    20821 gaagtccgtc atttcctaga aggagatgga catcagaata tccgagctca ctggccttgt
    20881 ctacgatgcg tttgagctgg ttcagagtaa agtatttgcg tccagcatcg attgagatta
    20941 ccttgttttt ggcaagtttt tcaacctcac gtttagcttc ttcttctttt tgagcttcag
    21001 gcgtgagggt caagttgttg acagtttctt gaagtttagc aatggcttga tcaatcgtat
    21061 cttgttgggc acggctaagg ttgctatcga gagagcgaat agctttttca gcttctttta
    21121 cggccgtgac gctttctgca gtataacggt tcaggtcttt tggtacctcg ttaagtgctt
    21181 gctctgcaga ttcataatca gctgcgaagt attcagcgtt ggcatttgca aaatgacgca
    21241 tgagtttgaa gaggcgtgat ggtgaataac gtgcagatgg agtgtcagcc caagcagcta
    21301 ccataccacc gatgattggg atatcagctc cttctgtttt tggtacagaa gtgattggtg
    21361 tgtttttaat accattgagc ccctgatcga gattgtacca gccttggcca tcagcgtttc
    21421 gtccaagaac gtagtaccaa gcatcattgg tattaaggat ttggtgacct ttttcagcta
    21481 gtagtttaga agaagcgaca tcgtagcctc cccaaccacc agtccacata gaaacgatga
    21541 tgtctttgtc aaaactacca aagcttgtgt cgctattgta gtagataccg tcgttaaagg
    21601 ccattggttt gagaccgtgc gattttacaa tacgagcgag gtcattggcg taggcaataa
    21661 atttttcata gccttttaca gggtagcctt cgtttggata gtatttatca gcttgaagca
    21721 cactccaacc tttagcatct gtcgcatcat tggcatattc atcaagtccg atgttgaaga
    21781 tttcagtctt tttcgcgaaa taagcagcat acttgtcgat aagggctttt gtaaaagcga
    21841 cagcttgttc gttgtcaaga ttgacagtac gggctgattc cttcccaaaa tagctaaagt
    21901 tagggttttg gattcccaat tctttcatgg cattgagaat cgcatccatg tgcccaggac
    21961 tatttactgt cggaatgaga ccgatacctt tatctttggc atagttaatc agatctgtca
    22021 tttgactttc tgttaagtga ttgccgtttg gatcgtcgta ataatcattt gtaccttttt
    22081 caatggcgcg tttgacatcg tcactggcat aggtcttgcc gttagctgtg atgctcatat
    22141 cgtccaacat gaaacggagt ccatcatttc cgactaatag gtgtaaatca gtgtagccat
    22201 aatgtttcgc tttatcgatg atttccttga gctgttctgg tgagaaatat ttacgtccag
    22261 catcaataga aacaattttc tttttcgcta gtttttcatt tacagttgca gcacgttcct
    22321 ttcctgcctc tgtttccggt ttgtcagcct ctgctttcgc ttcatctttt ttagctggtt
    22381 tatccttgtc agtcttgtct gtatttgact ctttagaatc aacctctttc gcttcttcct
    22441 ttttagggct agcttcttct gcctttttat tagcagtttc tttttcagca gaagttggag
    22501 ttaccacttc tgctttatca ctaggagttg aactaacttc ctcttgtggt ttttcttctg
    22561 tttttggaag actagctacc ttatcagtag ctggagtttc tgtttctaca gtttttggag
    22621 cttctggttg aagctctgct ttaggtgttt cctcagtccg attttcggat gattgagggg
    22681 aatcagaaac cgtatggatg gtcggttggt tttctgtagt agtaggagta actccatcgg
    22741 ctgcaacagt ctgtgcttgg aaggcaaatc caattagaac agaagctgct cctacagcgt
    22801 atttacgaat agaaaaacgc tgttgttttt catgtttcat tgcaaaacct cctgattgca
    22861 ttgttatatt gatagcgatt atataaatca acgcctttat tttatttctt atattaacga
    22921 gagtcaagag gagatgacaa aaaactataa taagtataaa aaaatataaa atttaaactt
    22981 aagatttcag attggtcgaa aaatacatat atatatctag tataattttt ggttctattt
    23041 ctataaaata ttccacaaat tatagaatct tccaaaaata ggtaagcgct acctttttgg
    23101 tgtggtataa taagcataga aaaagcccga gcgattaggc tcaggctttc ttcttagtga
    23161 tcacggtcac atgagatgaa ttcaatcttg tagtaatcag atcgtttgta agtttcactg
    23221 tattctaaaa cttggccagt tgattcgagt ttggtgattt tagtttgtag gacagtaggg
    23281 aattgttcat cgactccgag gactgaagcc gcatgttctg gagttggaaa gactatttcg
    23341 ttgatttctt caaagtgttc atcattcatg tgaatgtggt agtctaactt gaaacgatta
    23401 tagatagaac tatagtattc aaggtttgga taatttgcgt tgatatattg ttctgggatg
    23461 taggatgtat ggtagatata aacgacaccg tttgattcgc ggatacgttc aatcttgtag
    23521 tagaattgat cgccgcgtag acccaatttt tccaagtaaa caagcttgtt tccgcgttca
    23581 attgaaagaa cagttacctt atcatcttta gcattgaaga gttcaatatc tgaaaactct
    23641 acaagcttgt gtttgcgtgc acgtgaaacg aaggttcctt ttccttgttg gcggacaata
    23701 tagccatctt tggcaaggtc gtttaaggcg cgaacaactg tgatagagct gacatcgtac
    23761 attgaaatga gttctgcttc agtgtaaaat ttatctccac tgctaaactg cccagagatg
    23821 attttatttt ttaattcgtc ttttatgtat tgatatttgg gaattgccat atttttacct
    23881 ctattttcct tctccgtaga aaattttacc ataaaagcga aaaaaatagt agtcttttga
    23941 ataaaaaatt agaataatag tttgaaaaat taagcgatat agggattgct catattttat
    24001 caatgtattc tatacaaaat tattttaggt cctatattcc gaatttaaag agcgtttgaa
    24061 atgggtctaa aaatgtttga aactaattga atgtgtgtat aatagtaaaa atgcagtaaa
    24121 tatcccgaca atttctattg acaattcaaa cagattggtt tataattaat ataacaacaa
    24181 atgaaagcgc aaactttcgc ggtcggaagg tagttttatg acacgatttg agatacgaga
    24241 tgatttctat ctcgatggaa aatcatttaa gattttatct ggtgccattc attattttag
    24301 ggttcctcca gaggattggt atcattcgct ctataacttg aaggctcttg gttttaatac
    24361 ggtagagact tatattgctt ggaatttaca cgagcctcgt gaaggtgagt ttcattttga
    24421 cggtgatctg gatttagaga aatttctcca aatagcgcag gatttgggtc tctacgcaat
    24481 tgtgcgtccg tctccattta tctgtgcgga atgggaattc ggtggcttac cagcttggct
    24541 cttgaccaag aacatgcgaa ttcgctcatc cgacccagca tatatcgagg cagttggtcg
    24601 ctactatgat cagttattgc caagactggt gcctcgtttg ttggacaatg gtggcaatat
    24661 tctcatgatg caggttgaaa atgagtatgg ttcttacgga gaagataagg cttacctgag
    24721 agcgattcga cagctaatgg aagagcgtgg cgtaacctgt cctctcttta catcagatgg
    24781 tccatggcga gctactctga aagctggaac cttaattgaa gaggacctct ttgtaacagg
    24841 aaactttggt tctaaggcac cttacaactt ttcgcagatg caggaattct ttgatgaaca
    24901 tggtaagaaa tggccactca tgtgtatgga gttctgggat ggttggttta atcgctggaa
    24961 agaaccgatt atcacacggg atcctaagga attggcagat gcagttcgag aggttttgga
    25021 acaaggctct atcaatcttt acatgttcca cggtggtaca aactttggtt tcatgaatgg
    25081 ttgctcggct cgaggaactt tggacctgcc acaagttacg tcttatgatt acgatgccct
    25141 tctggatgaa gaaggaaatc caactgctaa atatcttgca gtcaagaaga tgatggcaac
    25201 acatttttca gagtatccgc agttggaacc actctacaaa gagagtatgg agttggatgc
    25261 tattccacta gttgaaaaag tttctttgtt tgaaacctta gatagcttgt caagtcctgt
    25321 agaaagtctc tatcctcaaa agatggagga gctgggacaa agttatggct acctacttta
    25381 tcgaacagaa acaaactggg atgcagaaga agaaagactt cgtatcattg atggtcgaga
    25441 tagggcccag ctgtatgtcg atggtcagtg ggttaaaact caatatcaga cagagattgg
    25501 ggaagatatt ttttatcaag gtaaaaagaa agggctatct aggttagata tcttgataga
    25561 aaatatgggg cgtgtcaact atgggcataa gttcttagcg gatacgcaac gtaagggaat
    25621 tcggacaggg gtctgtaagg atctgcattt cttactaaac tggaaacact atccacttcc
    25681 actagacaat cctgagaaaa ttgatttttc aaaaggatgg actcaaggac aaccagcctt
    25741 ttacgcttat gactttacag tcgaagagcc aaaagatact tacctagact tgtctgagtt
    25801 tggtaaggga gttgcctttg tcaatgggca gaatctagga cgtttttgga acgttggccc
    25861 aactctctca ctttatatcc ctcatagcta tctcaaggaa ggtgccaacc gcatcattat
    25921 ctttgaaaca gaaggtcaat ataaagaaga gattcattta actcgtaaac ctacactaaa
    25981 acatataaag ggggaaaact tatgacaatt gtaggatgcc gtattgatgg acgtttgatc
    26041 cacggacaag tagccaatct ttgggctgga aaactaaatg tttcacgcat tatggttgta
    26101 gacgacgaag ttgtcaacaa cgatattgaa aagagtggtt tgaaacttgc gacaccacca
    26161 ggtgtgaaat tgagtatttt gccagttgag aaagctgcag ccaatattct tgctggtaaa
    26221 tacgatagcc aacgtctctt tatcgtggct cgtaaaccag accgcttcct tggcttggta
    26281 gaagcaggtg taccacttga aacccttaat gttgggaata tgtctcaaac accagaaact
    26341 cgttctatta cacgttctat caacgtagta gacaaggatg tggaagactt ccacaaactg
    26401 gcagaaaaag gtgttaaact tactgctcag atggttccaa atgatccaat ttcagacttt
    26461 ttgagcttat taaaatagga aaaaaatttt taggaggtca ttgttatgat acaatggtgg
    26521 caaattttac ttctcacttt gtactcagct tatcaaatct gtgatgagtt gacgatcgtt
    26581 tcatctgcag gttcccctgt atttgctggt ttcattactg gtttaatcat gggagatgtg
    26641 actactggtt tacttatcgg tggtaacttg caactgttcg ttcttggggt tggtaccttc
    26701 ggtggtgctt ctcgtatcga cgcaacttct ggtgcggttc ttgcgacagc cttctctgtt
    26761 tcacaaggaa ttgatgcacc gcttgccatt actacaatcg ctgtaccagt agcagctctc
    26821 ttgacttact tcgacgttct tggtcgtatg actactacct tcttcgctca ccgtgtggat
    26881 gctgcaatcg aacgctttga ctataaaggt attgaacgca actacttgct tggtgcgatt
    26941 ccgtgggctc tatctcgtgc ccttccagtc ttctttgccc ttgcttttgg tggtgccttt
    27001 gtacaatcag tagtagactt cgttgaagcc tacaaatggg ttgcagatgg cttgacactt
    27061 gcaggacgta tgcttccagg tcttggattt gcaatcttgc ttcgttacct tccagttaaa
    27121 cgtaaccttc actaccttgc tatgggattt ggtttgacag ctatgttgac tgttctttac
    27181 tcatatgtaa caggtcttgg tggcgctgtt gctggtatcg taggtactct tcctgctgaa
    27241 gttgctgaaa aaattggttt cgtgaacaac ttcaaaggtt tgtctatgat tggtatttct
    27301 atcgtaggta ttttccttgc agtgcttcac ttcaaaaata gccaaaaagt agctgtagca
    27361 gcaccttcta caccatcaga aagtggggaa atcgaagatg acgaattcta attacaaact
    27421 tacaaaagaa gattttaatc aaatcaacaa acgtagcttg tttactttcc aattaggttg
    27481 gaactacgaa cgtatgcaag cttctggtta cctttacatg atcttgcctc agttgcgtaa
    27541 aatgtatggt gatggaactc ctgaattgaa agaaatgatg aaagttcata ctcaattctt
    27601 caatacttca ccattcttcc ataccattat cgctggtttt gaccttgcca tggaagaaaa
    27661 agatggtgta ggttcaaaag acgctgttaa cggtatcaag acaggtttga tgggaccatt
    27721 cgctcctctt ggggatacaa tctttggttc acttgtacct gctatcatgg ggtcagtcgc
    27781 agcaactatg gctatcgctg gccaaccttg ggggatcttc ctttggattg cagttgcagt
    27841 agcgtatgac atcttccgtt ggaaacagtt ggaatttgct tacaaagaag gggttaacct
    27901 tatcaacaac atgcaaagta ccttgacagc tttgattgac gctgcatctg tacttggtgt
    27961 cttcatgatg ggtgctcttg tagcaacagt gattaacttt gaaatttctt acaagttgcc
    28021 aatcggtgaa aagatgattg atttccaaga catcttgaac caaatcttcc cacgtttgct
    28081 tccagcaatc tttactgcct ttatcttctg gttgcttggt aagaaaggta tgaactctac
    28141 taaagctatc ggtattatta tcgtacttgc tttggctctt tctgcccttg gtcactttgc
    28201 acttggaatg taattcctta tgactaaatc attaattttg gtgagccatg gtcgcttctg
    28261 tgaggagctt agaggtagca cagaaatgat tatgggccca caagacaaca tttacacagt
    28321 agctcttctt ccagaagatg gcccagaaga atttactgct aaatttgaag ctgttattga
    28381 aggattggat gatttcctag tttttgcgga tcttctcggt gggacacctt gtaatgtggt
    28441 gagtcgcttg atcatggaag gtcgtgatat tgacctttac gcagggatga atcttccaat
    28501 ggtgattgaa tttatcaatg cgagccttac aggcgcagat gcggactaca agagccgtgc
    28561 tgcagaaagc attgtgaaag ttaatgacct gttagcgggc ttcgatgatg acgaagatga
    28621 ataatactct tcgaaaatct cttcaaacta cgtcaacgtc gccttaccgt aggtatatgt
    28681 tactgacttc gtcagtctta tccggcaacc tcaaaacggt gttttgagct gacttcgtca
    28741 gtcttatccg gcaacctcaa agcagtgctt tgagcagcct gcggctagtt tcctacagat
    28801 tttagttgga actcgattca attcatgtga caacgtgaaa atcgttagag cattttatat
    28861 agaatataca tgggaatgga gcttactccc attcctatat ttaatagaaa aagaggaact
    28921 caatgctaca ttatacaaaa gaagacttgc tcgaattggg tgcagaaatc actacgcgtg
    28981 aaatctacca acagcctgat gtatggagag aagcttttga attttatcaa gcaaaacgtg
    29041 aagaaattgc agccttccta caagaaatcg ctgataaaca tgactatatt aaggttatct
    29101 tgacaggtgc tgggacttct gcttatgtgg gagacacctt gctaccttac tttaaggaag
    29161 tctatgacga acgcaaatgg aatttcaatg ctattgcgac aacagatatc gttgccaatc
    29221 cagcaaccta tttgaaaaaa gatgtggcaa ctatccttgt gtcttttgct cgtagtggga
    29281 actcgcctga aagtgtggcg actgttgatt tggccaaatc cttggtggat gagctttatc
    29341 aagtgacgat tacttgtgca acagatggta aattggctct tcaagctcac ggtgatgacc
    29401 gtaatctctt gctcttgcaa ccagctgtct ctaatgatgc tggatttgcc atgacttcta
    29461 gctttacgtc tatgatgttg acagctctct tggtctttga tcctacagaa tttgctgtta
    29521 agtctgaacg ttttgaagtt gtatctagtc ttgcccgtaa agttttagac aaggcagaag
    29581 atgtcaaaga gctcgttgat ttagacttta accgtgtcat ctatctaggc gctggtcctt
    29641 tctttggact tgctcatgaa gctcagctca agattttgga attaactgct ggtcaagttg
    29701 cgaccatgta tgaaagccca gttggcttcc gtcacggtcc aaaatctctt atcaacgaca
    29761 atacagttgt tttggtcttt ggtacaacga cagactacac tcgtaagtac gacttggact
    29821 tggttcgtga agttgctggt gaccagattg ctcgtcgtgt tgtgcttttg agtgatcaag
    29881 cttttggtct tgaaaatgtc aaagaagtgg cccttggttg tggcggtgtc ttgaatgata
    29941 tttaccgtgt cttcccttac atcgtttatg cccaactctt tgccctattg acttcactca
    30001 aggtagaaaa taaaccagat acaccgtctc ctacaggtac agtaaaccgt gtagtacaag
    30061 gtgtcataat tcacgaatat taaaagtaag acagtgttta tgaattcttg acaagaggat
    30121 ttgtaaatta tcagataaac catagattgt cagtacgctt tctatggttt gtttgcttga
    30181 gagaaatagt aaaaggagaa cagaatgaaa gcatacacag agcgtgtatt tggaaatgtt
    30241 gagggcgagg atgtcttggc ctatcgattt gagacagacg gtggctacca acttgaggtt
    30301 atgacttatg gtgcgaccat cttgcgctat gtcgcacctg acaaggctgg aaattttgcc
    30361 aatgttatct tgggatttga tgactttgat agttatgtag gcaatagtcc caagcatgga
    30421 gcaagtgtag gtcctgtagc gggtcgtatt gcaggtgcga cctttgagct caatggtaag
    30481 acctatgacc ttgaggttaa taatgctagc aactgtaatc acagtggttc aactggttgg
    30541 gattccagct tgtttgaagt tgaagaagta agcgatcatg gcttgactct ctacacagag
    30601 cgtacagatg ggacaggagg gttccctgga aatctcaaga tttggattag ttatcacttg
    30661 gaagaaactg gtgcctatga aatcagctac aaggtaacga ccgatcagga tacgctggtc
    30721 aatccaacca accacagcta tttcaacttg tctggtgatt tcacgcagac gattgaccgt
    30781 catgtcttcc aactaaacac agagggcatt tactcaatcg ctcctgacgg tgttcctgcc
    30841 aaaactccag aagccaaccg tgatgtggtc aaacacatct acaatggtgc cttgttgaag
    30901 gatatctttg cagaagaaga tgagcaaatc cagctggcat caggtttgga tcatccattt
    30961 gcccttcctg caggccatga caatgctgga ttcctttatg accaaaattc aggtcgcttc
    31021 ctgcttttca agacagaagc tccttgcttt gtggtctaca cagcaaactt tgtggatgaa
    31081 agtgtcatca taggaggtca gccaatgcta cagcacaatg ggattgctct tgaagcgcaa
    31141 gctttaccag atgccattca cagtgacctt aaaggtcaag tcattcttaa agctggtcaa
    31201 accttcacca gtaagacacg ttatgaactt gttgtgaaat aaaaaagctg gtcaaaaagt
    31261 ctttaaaatt aaaataacgc atattgtcag gtgatgaaaa gtttgataat atgcgtttta
    31321 ttatgaaatg attt
//
LOCUS       DAVDWM010000022        30222 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVDWM010000022 DAVDWM010000000
VERSION     DAVDWM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.022"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            200..2092
                     /gene="pepO"
                     /locus_tag="VUW49_001183"
     CDS             200..2092
                     /gene="pepO"
                     /locus_tag="VUW49_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET7981106.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVSEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEESWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2215..2844)
                     /locus_tag="VUW49_001184"
     CDS             complement(2215..2844)
                     /locus_tag="VUW49_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7981107.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(3184..3426)
                     /locus_tag="VUW49_001185"
     CDS             complement(3184..3426)
                     /locus_tag="VUW49_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981108.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            3837..6059
                     /locus_tag="VUW49_001186"
     CDS             3837..6059
                     /locus_tag="VUW49_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7981109.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            6089..6532
                     /locus_tag="VUW49_001187"
     CDS             6089..6532
                     /locus_tag="VUW49_001187"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET7981110.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6571..6985)
                     /locus_tag="VUW49_001188"
                     /pseudo
     CDS             complement(6571..6985)
                     /locus_tag="VUW49_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7004..7417)
                     /locus_tag="VUW49_001189"
     CDS             complement(7004..7417)
                     /locus_tag="VUW49_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981111.1"
                     /translation="MKKLYRIHFIAISVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(7548..8060)
                     /locus_tag="VUW49_001190"
     CDS             complement(7548..8060)
                     /locus_tag="VUW49_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981112.1"
                     /translation="MVVGIAIASGYLNSRLDKFVDWGLWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGATDYILGNKAAIITYPVTFLIYTFAIKKWKASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLNAIGA
                     LFVWNDEEVR"
     gene            complement(8237..8887)
                     /locus_tag="VUW49_001191"
     CDS             complement(8237..8887)
                     /locus_tag="VUW49_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7981113.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            8999..9841
                     /locus_tag="VUW49_001192"
     CDS             8999..9841
                     /locus_tag="VUW49_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7981114.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQSLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9917..10354
                     /locus_tag="VUW49_001193"
     CDS             9917..10354
                     /locus_tag="VUW49_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7981115.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10422..10724
                     /locus_tag="VUW49_001194"
     CDS             10422..10724
                     /locus_tag="VUW49_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981116.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYIFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10815..11888)
                     /locus_tag="VUW49_001195"
     CDS             complement(10815..11888)
                     /locus_tag="VUW49_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET7981117.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            12044..12721
                     /locus_tag="VUW49_001196"
     CDS             12044..12721
                     /locus_tag="VUW49_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981118.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12714..13688
                     /locus_tag="VUW49_001197"
     CDS             12714..13688
                     /locus_tag="VUW49_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET7981119.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13737..15680)
                     /gene="thrS"
                     /locus_tag="VUW49_001198"
     CDS             complement(13737..15680)
                     /gene="thrS"
                     /locus_tag="VUW49_001198"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7981120.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQFISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15840..16061)
                     /locus_tag="VUW49_001199"
     CDS             complement(15840..16061)
                     /locus_tag="VUW49_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7981121.1"
                     /translation="MELQELVEHSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIW"
     gene            complement(16492..16692)
                     /locus_tag="VUW49_001200"
     CDS             complement(16492..16692)
                     /locus_tag="VUW49_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981122.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            16929..17558
                     /locus_tag="VUW49_001201"
     CDS             16929..17558
                     /locus_tag="VUW49_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7981123.1"
                     /translation="MKLAVIAANGQVGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17699..17968
                     /gene="rpsO"
                     /locus_tag="VUW49_001202"
     CDS             17699..17968
                     /gene="rpsO"
                     /locus_tag="VUW49_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7981124.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18783..19397
                     /locus_tag="VUW49_001203"
     CDS             18783..19397
                     /locus_tag="VUW49_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET7981125.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19690..20439)
                     /locus_tag="VUW49_001204"
     CDS             complement(19690..20439)
                     /locus_tag="VUW49_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7981126.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20572..22908
                     /locus_tag="VUW49_001205"
     CDS             20572..22908
                     /locus_tag="VUW49_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7981127.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGAIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQEKK"
     gene            23437..24900
                     /locus_tag="VUW49_001206"
     CDS             23437..24900
                     /locus_tag="VUW49_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET7981128.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSAFLTEGDKQRLQAKIQEINYE"
     gene            24893..25321
                     /locus_tag="VUW49_001207"
     CDS             24893..25321
                     /locus_tag="VUW49_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7981129.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKPPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            25299..25748
                     /locus_tag="VUW49_001208"
     CDS             25299..25748
                     /locus_tag="VUW49_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7981130.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            25776..26087
                     /locus_tag="VUW49_001209"
     CDS             25776..26087
                     /locus_tag="VUW49_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET7981131.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            26113..27198
                     /locus_tag="VUW49_001210"
     CDS             26113..27198
                     /locus_tag="VUW49_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HET7981132.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            27209..27904
                     /locus_tag="VUW49_001211"
     CDS             27209..27904
                     /locus_tag="VUW49_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7981133.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            27941..29911
                     /gene="tkt"
                     /locus_tag="VUW49_001212"
     CDS             27941..29911
                     /gene="tkt"
                     /locus_tag="VUW49_001212"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7981134.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHCGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
BASE COUNT         8840 a         5523 c         6842 g         9017 t
ORIGIN      
        1 agattctaaa gataactttc aggataaatt tctaaattat aaaacgcata gtatcaagtg
       61 taaaaaactt ggaattatgc gttttatcat ggaaagattt tttataatag ctaaaaaata
      121 atagttaaat ggttattaat tgcattccct agtgattttt gttaagataa aggcaaatac
      181 aaatgaaagc gagaacaaga tgacacgtta tcaagatgat ttttatgatg ctatcaatgg
      241 agaatggcaa cagacagctg aaatcccagc agataagtct caaacaggag gttttgttga
      301 tttagaccag gaaattgaag acctgatgct ggcgacaaca gacaagtggt tagcaggtga
      361 agaggtgtct gaggatgcta tcttggaaaa ctttgtcaaa taccaccgcc tagttcgtga
      421 ttttgacaag agagaagctg acggtatcac acctgtctta ccactcctta aagaattcca
      481 agaattggaa acttttgcgg attttacagc taaactagca gagtttgagc ttgcaggaaa
      541 accaaacttc cttccttttg gtgtatcgcc agactttatg gatgctagaa tcaatgttct
      601 atgggctagc gctccaagca caatcttgcc agatacgacc tactatgcag aagaacatcc
      661 tcagcgcgaa gaactcttga ctctttggaa agaaagcagc gcaaatctcc tcaaggctta
      721 tgatttctct gatgaagaaa ttgaagactt gctagaaaaa agacttgaat tggaccgccg
      781 agttgcggca gtggtactct ctaatgaaga aagttcagaa tatgctaaac tctatcatcc
      841 atattcttac gaagatttca agaaattcgc gcctgcccta cctttggatg acttcttcaa
      901 agcagttatt gggcaattac cagacaaggt tattgtagac gaggaacgtt tctggcaagc
      961 agcagagcaa ttctacagtg aggaatcctg gtctctcctt aaagcaacct tgattttgag
     1021 tgttgtcaat ctttcaacca gctatttaac agaggatatc cgtgttttgt ctggtgccta
     1081 cagccgtgcc ctttctggag ttccagaggc aaaagataag gtcaaagcag cttatcatct
     1141 agcacaagaa cctttcaagc aagccctggg tctttggtac gcccgtgaga agttctctcc
     1201 agaagccaag gcggatgtgg agaaaaaagt ggcaaccatg attgatgttt ataaggagcg
     1261 tctgcttaag aatgactggc tcactccaga aacctgtaaa caggctatcg tgaagctcaa
     1321 tgtgatcaaa ccttatattg gctatccaga agaattgcct gcacgttaca aggataaggt
     1381 agtgaatgaa actgccagtc tttttgagaa tgctctagcc tttgcgcgtg tggaaatcaa
     1441 gcacagttgg agtaagtgga accagcctgt agactataag gaatggggca tgcctgctca
     1501 tatggtcaat gcctactaca atcctcagaa gaacctgatt gtctttccag cggccatttt
     1561 acaggcgcct ttctatgact tgcatcagtc atcttctgct aactacggtg gtattggggc
     1621 agtgattgcc catgaaattt cccacgcctt tgatactaac ggggcttcct ttgacgaaaa
     1681 tggtagcctc aaggattggt ggacagagag cgactatgct gccttcaagg agaaaacaca
     1741 aaaagtcatt gaccaatttg atggacagga ttcttatgga gcaaccatta acggtaaatt
     1801 gactgtatca gaaaacgtgg ctgacttggg aggaatcgca gcagctcttg aagcagctaa
     1861 gagagaagca gacttctcag cagaagagtt cttctacaac ttcggtcgta tctggcgcat
     1921 gaaaggtcga ccagaattta tgaaactttt ggctagcgtc gatgtgcacg caccagccaa
     1981 actccgtgtc aatgtgcaag taccaaactt cgacgatttc tttacaacct atgatgtcaa
     2041 agaaggagac ggaatgtggc gttcaccaga ggagcgcgtg attatttggt aatgcaaaaa
     2101 atctagtcat cagaaaaaag gcatccaatc atgtgtgaaa accttgatag gatgcctttt
     2161 gtaatagaaa gatttgctgg atagtttact taaatttcga ttgtcatcat cttgctaaaa
     2221 aaagggattg aaggcctttt cgtgagcgat agtagtagct ggaccatgtc ctggatagac
     2281 atcgtagttt ggtagggtga agagttgggt ctggatacta tgaaggagtt gctccatgct
     2341 accagtcgga aggtcggtcc gtccgatagt ttcgcggaat agagcatctc ccgtcaagac
     2401 taggtgagca tcaggaaaga ctagggaaac accaccgata gagtgccctg gggtcggtag
     2461 aaccttaaaa cgaaattcct ctagttggta ttcttcgtga aagacaaagg tgtgttctgc
     2521 aggttttgtg accacatctg ccatatcatc gtggcgaggg agaccggaga gattatcgac
     2581 aggagtgtag agccagctgg cttcgctctc tgcgatatag acaggaggat tgccaaacgt
     2641 ctcgcgaacc aagtccagac tcatgatatg gtcataatgg gcgtgggtca agagaatagc
     2701 acagatcggt ttgttgatct tctcgattgt ctgacgaatg gcttcccaat gactaccagg
     2761 atcgacgacg atgaggtgct tttcgccttc tagataatag gtattttcat aggcaacagg
     2821 attcacggtt ttatggattt tcatactagc tccaatctca aagaatgtac taatactctt
     2881 cgaaaatctc ttcaaaccgc gtcaacttta tctgcaacct caaagcagtg ctttgaacag
     2941 cctgcggcta gctttctagt ttgctctttg attttcattg agtataaatt aagtatagca
     3001 cagttaggga gaataggtaa ggatttaaaa tgaacttaaa aaccatcatg actagatgaa
     3061 gacaggctgg ttatcaattt atcaatattg gaaaggtgtc aattgccctt cataggttgc
     3121 ataaactccg tcattagctt gtttgataga cgtgatgttt agagtgctac cgtagttggc
     3181 ttctcatttg tcgctatatt cactataagt tatcctactg ggcaggttct ggtcgttagc
     3241 atagtattga acgagttttt tatagctttg ctggctgaat aagaacaggc agcaaactcc
     3301 taacattagg agagcaaaga tagaaatagc agttttttca taggtattct ccttataaac
     3361 taaaggatag aagaaagtat cttgaggatt tttatactct tcaaaaatct cttcaaaccg
     3421 cgtcaacgtc gccttgccgt atatatgtga ctgactttgt cagtcttatc tacaacctca
     3481 aagcagtgct ttgaacagcc tgcggctaat ttcctagttt gctcttggat tttcattgag
     3541 tattaattcc aaaatccagt caaggtacca gtgtatgtga tacgagttcc accgtttatc
     3601 tttcgtatct tcaatggaat tggggaagtc agaaagtctt tgggttacaa tatggtttcc
     3661 gttcttctat ctctccttct atcacctaag tttaaaatct aacttcgtag tatattatgg
     3721 accaataatt cagaattatc aagcaatttt gaattatttt tttcttattc tgttccaatc
     3781 tagttttcat attcttaaaa aagtgataaa atggtaggaa gaattggaga gtagagatgc
     3841 cgaaagaagt gaatttaaca ggcgaagaag ttgtcgcttt aaccaaagaa tatttaacgg
     3901 aagaggatgt tcattttgtc cataaggcct tggtctatgc tgttgaatgc cacagtggtc
     3961 aatatcgcaa atcaggcgag ccttatatca ttcaccctat ccaagtggca ggtattttag
     4021 ctaagctaaa gctggatgct gtaacagtag cttgtggatt cttgcatgat gtggtggaag
     4081 atacagatgc gactttggac gatttggaaa gagagtttgg tcctgatgtg cggatgattg
     4141 ttgacggagt taccaagctt ggcaaggtcg agtacaaatc gatcgaggag caattagcgg
     4201 aaaatcatcg caagatgctc atggccatgt ctgaggacat ccgcgttatt ttggtcaaac
     4261 tgtctgaccg cttgcacaat atgcggaccc tgaaacatct tcgaaaagac aagcaggagc
     4321 gtatttccaa agaaaccatg gaaatctatg ccccgcttgc ccatcgtttg gggatttcca
     4381 gtgtcaaatg ggaattagaa gacttgtctt tccgttatct caatccaacg gagttttaca
     4441 agattaccca tatgatgaag gaaaagcgca gggagcgtga ggccttggtg gatgaggtag
     4501 tcacaaaatt agaggagtat acgacagaac gtcacttgaa agggaagatt tatggtcgtc
     4561 ccaagcatat ttactcaatt ttccgcaaaa tgcaggacaa gagaaaacgg tttgaggaaa
     4621 tctatgatct gattgctatt cgttgtattt tagataccca aagtgatgtt tatgccatgc
     4681 ttggttacgt gcatgaattt tggaaaccga tgccaggtcg cttcaaagac tatatcgcca
     4741 accgcaaggc caatggttat cagtctatcc atacgactgt ttatggacca aaagggccga
     4801 ttgaattcca gattcgaacc aaggaaatgc acgaggtggc tgagtacggg gttgcggctc
     4861 actgggctta taagaaaggt ataaaggggc aagttaacag caaggaatca gctattggaa
     4921 tgaactggat caaggagatg atggagctcc aagaccaggc tgatgatgct aaggaatttg
     4981 tggactctgt taaggaaaac tatttggctg aggagattta cgtttttacc ccagatggag
     5041 ctgtccgttc ccttcccaaa gattcaggac cgattgattt tgcctacgaa atccatacca
     5101 aggtcggtga aaaagcaact ggtgccaagg tcaatggccg catggttcca ctgacaacca
     5161 agttaaagac aggggatcag gttgaaatta tcgccaaccc gaactccttt ggacctagcc
     5221 gtgactggct caatatggtc aagactagca aggcgcgcaa taagattcgc cagttcttta
     5281 aaaaccaaga taaggaattg tctgtcaaca agggtcgtga gatgctgatg gctcagttcc
     5341 aagaaaatgg ctatgtggca aataaattta tggacaagcg ccacatggat caagttctgc
     5401 aaaagaccag ttacaagaca gaagactccc tctttgcggc cattggtttt ggggaaatcg
     5461 gtgcgattac cgtctttaac cgtctgactg aaaaggaacg ccgtgaggaa gagcgtgcca
     5521 aggccaaggc tgaggcagag gagcttgtca aaggtggcga ggtcaaggtt gaaaataaag
     5581 aaaccctcaa ggtcaagcat gaggggggag tggttattga aggtgcttct ggtctcctag
     5641 tgcggattgc taagtgttgt aaccccgtgc ctggtgacga tattgttggc tacattacca
     5701 agggtcgtgg tgtggctatt caccgtgtgg actgtatgaa cctgcgtgcc caagaaaact
     5761 acgagcaacg tctccttgat gtggaatggg aagaccagta ctctagctca aataaggagt
     5821 atatggccca tatcgatatc tacggtctca accgtacagg actgttgaac gatgtactgc
     5881 aagttctttc aaatacaacc aagaatattt caacggtcaa tgcccaacca accaaggata
     5941 tgaagtttgc taatatccat gtgtccttcg gtattgccaa cctctctaca ctgaccacgg
     6001 ttgtcgataa aattaagagt gtgccagaag tttactctgt caaacggacc aacggctagt
     6061 tgtcctagct cttactagaa aggctattat gaaaatcatt atccaacggg ttaaaaaagc
     6121 ccaagtgagt atagaaggcc agattcaggg aaaaatcaat cagggacttt tattgctggt
     6181 tggtgttgga ccagaggacc aagaggaaga tttggactat gctgtgagaa aactggtcaa
     6241 tatgcggatt ttttcagacg cagaaggcaa gatgaacctg tctgtcaaag atattgaagg
     6301 agaaatcctc tctatttctc agtttaccct ctttgcggat actaagaaag gcaatcgtcc
     6361 agcctttaca ggggcagcta aacctgatat ggcatcagac ttctatgatg ctttcaatca
     6421 aaaattagcg caagaagtgc ccgttcagac aggtatcttt ggagcagata tgcaggttga
     6481 gctggttaat aacggacctg ttaccattat cctagatact aaaaagagat aagaaagacc
     6541 aagcccagtc ggcttggtct ttctcatcga tcataaaata ctccaaaaag aaatcggttc
     6601 ttgatatgct tgggggaagt ctcttttcag gctttggcag atgcgatagg aagggatgag
     6661 atgtcctagg atgaggagag ttccctgaag gaaaagtgga atatcactta aagacaccaa
     6721 ggagagaatc atcaggctat cccataggag aatttgtaaa gcaaaacgcc agaatctcgg
     6781 tctaatctgg gaatagagtt gactaaagtg gtcacaaagt tggtttgaaa gataggtcaa
     6841 tagcacagga tgaaagaaaa tgagccaacc actataaatc aaaatccagt cccaagtaag
     6901 cccccctttt aaatgtcaaa aatgccagca tgattccatc aatgacaagg agttgaatgg
     6961 ttttgatgaa gatgattttt ttcatgctaa aacctccttt tccttagaga cactcccata
     7021 aagagatgtg tttgtcgtaa aagtctggat agttttctct taggtagtca gctgtcatat
     7081 aataaaaagt agaatggcaa gcagtgataa ttgacataag agatgaaaaa cgctgagaac
     7141 taatgaaggc taagagaaca aaaaatagaa aatcaatggt gagaacccct aaatagtaga
     7201 agcgaatctt tttattgatt tgaggataaa tctcagcgaa ttgatgtttg agtcggacga
     7261 tcaagagaaa tagcaggagt ccttgagcaa ggaagaaaat taaaccagag agcaagagcc
     7321 actcaaaaga tgtctcgagt ctaatgataa aaaatgcaaa tagtagcaaa tcaataactg
     7381 aaattgctat aaaatggatt ctatagagtt ttttcatgac aagacctccc tcttttatct
     7441 aacttcattc tactctaaaa gaatgggagt tacaactaaa atgataaaaa tagcagaggg
     7501 gagattctct taagttggct agtattcttt atttgagttt tcttctatta tctaacttct
     7561 tcatcattcc agacaaataa agctccgatt gcattgaggg acagtgcaaa ggcaatagtt
     7621 gttacgtagg caaggagatt catcttgccc ccatatccga tatagttggt cacaaaggca
     7681 aagaggaagg cgatgatgga aatgatgatg gccgccaatt ttacctgttt ttggctcatt
     7741 tggttgggtc tgccttcttg cgaagctttc catttcttta tagcaaaggt ataaatgagg
     7801 aaggtgacgg gataggtaat gatggccgcc ttatttccaa ggatataatc agtagcaccg
     7861 gacaaaatgg tattaacaat accaaagtaa tttccccatt tgcttaattt ccccgtgaaa
     7921 cgagtggaca acatggaaat cccaacgttg gttacggaaa tcaatccaaa gggtacaaga
     7981 gctgtccata gtccccagtc tacaaattta tcgaggcgtg agttgagata accagatgca
     8041 atcgcaatcc caacgaccaa agcaaccccg aagaggtcaa atcatttaga tgtagcaaaa
     8101 atttttagtg attttttcat aggttaaact acctttcttt ttttcaaata ttctaccacc
     8161 aaatgaaagt aaaataaaat gatagaaata aaaccctgaa aataaaactt tcagggttgg
     8221 taggggactt gagaaattag ttgattttct cgacatagag ttgttttgca atgtccatat
     8281 tcacttgtaa atcctcgtcg ttactgagga tagtgaaggt attgctgaag ccatcaaact
     8341 gcttgacttg gagctggtca ccgatgtgaa gtgagtgctt gtccagataa tggagaatgt
     8401 caaaattatc gtgcacccga gtcaggcggt aggcgccagc ttccttgata tcagctagtg
     8461 ggaggttatt gatttcaacg aggagttctc ccttggcagg aatagttcct ccgtgggggc
     8521 aggttttagg gaaacctagc agtttatcta gtctttccac gaacaggtca gagacagtgt
     8581 gttccaagac ctcagcttcc tcgtgaatct ggtcacttgt atagtctaaa tgatgaacta
     8641 gaaaaacttc aatcaagcgg tgcttacgat agagctcaga gactagtttg agaccgaggt
     8701 cagtcagtag atagccacat tccttgtcct ttaggatgag gttttcactt ttcattcgtt
     8761 tgatcatttc agttacggca gggggagaga cttgcatgcg agctgcaatt tccttgttgg
     8821 taatcttatg caggtctatg ccaatttcat aaatacattt tagatagtct tctttgtttg
     8881 gggtcattcg tttcctcttg tctaatatct ttatctagta tatcaaaaaa agctagattt
     8941 ctctagctgt gactttttat gttatactta ttgcaagaga aaaaacgagg agatggatat
     9001 gacgaaaata gctcttcttt cagatattca tggaaatacc accgccttgg aggctgtttt
     9061 ggcagatgct cggcagatag gagtggatga atactggctt ttgggagata ttctcatgcc
     9121 agggacaggg cgtagaagga ttttggactt gttggatcaa ctaccgatta cggctagagt
     9181 tttgggaaac tgggaagaca gtctttggca tggtgtccgt aaggaattgg acagtactcg
     9241 ccccagtcaa cgctatctct tgcgccagtg ccagtatgtt ttagaggaaa tttccctaga
     9301 agaaattgaa gtgctccaca atcaatctct tcaaattcat cgtcagtttg gggatttgac
     9361 ggtgggaatt agccatcatc tgcctgataa gaactggggg cgagagttga ttcatactgg
     9421 caaacaagag gagtttgacc gcttggtgac tcatcctccc tgtgatattg ctgtttatgg
     9481 acatattcac cagcagttgc ttcgttacgg gactggtggg caattgattg tcaatccggg
     9541 ttcgattggc caacctttct ttttggacgc tcagctacgg aaggacttgc gggcccagta
     9601 tatgattttg gagtttgatg acaaaggcct ggtagatatg gacttccgac gggtagacta
     9661 cgatgtagca gctgaattgc agctggctaa agacctgaga cttccctatt ttgaggttta
     9721 ctatgaaagt ctggtcaatg ggatccacca tactcatcat caggaatttt tgagagaatt
     9781 ggctcagaaa gagggctgcg atcgggagtt agacgactgg ttgaaaagtg gtaacgattg
     9841 acattacaac taaaaagggt ataataatag aaaaagaaga gtagaggcag ggtagcctcg
     9901 taaaaaggag aagaggatgc aaattccaag tagatttacc attgcgactc atatgctgat
     9961 aatcattgcc ctcgagggga aggaaagcaa ggtgaccagt gattttctgg ctgctagtgt
    10021 cggggtcaat cctgtcatta tcagaaagat cttgtcccag ttgaagaagg cagagctgat
    10081 ttcagtagcg cgtggaacgg gcggaacaga gattgtcaag gaccttaagg atattagtct
    10141 tttagatgtt tatcaggcgg tcgaatgtct tggtaagaca ggtcaactct tcagtttcca
    10201 tgacaatccg aatccaaatt gccctgtagg agctcatatt catgatgttt tggatcaaaa
    10261 attggagaga attcagttga ctatggaggc agaacttggt caaaccagtc tagaaaaagt
    10321 cgtggccgat gcagagagtc agatgaagga ttaagaggga taaatgccct cttttttctt
    10381 tggataatta tgataaaatg tagctattaa aggagtaaga aatgtatctc gttataggaa
    10441 ttgtattagc ctttatagtg tcattttgga aggataatag aagtttgtgg aatccagtat
    10501 tatttttatt atccctcatt tcaagttatt tttatctatc ctaccttttt tataaaaatg
    10561 gatacgagaa tgttcagtta gctttttata tatttgcctt tgtcttactt ccttttttgc
    10621 tttttctaag tggcatcttt ttaatctata atggggtcat tttgttgaag cgagagggac
    10681 gttccaagcc ccattatctc tcaatgctct ttgattttta ttgactatta gattctattt
    10741 aaaaagactt agaatgcaca tgaaagtatg tattctaggt cttttgtttt gtatacattt
    10801 aattttcaag agtattatct atttttccat gcctggtaac gagtatcaat caataactgg
    10861 gtaaggcgac ccaaggtttc tctttcttct ggagtgaggg attgagcttg gacaaagaga
    10921 tgatgaatgt attcaagcgc ctttaaggaa gccaagtcat tgatagagct aataccagca
    10981 tcaagaatag tgccaaagaa gaggtcgaag tagagctgaa gttcttcgtc agggactgtg
    11041 gccacccatt ctttaaaggt tgtgtctact tgctggctat cactgttggt cttatccagt
    11101 tggacgaagt gcttgtcctc aatctgccaa ctaaaggtat cgtgctgggc gatgccaccc
    11161 agggcagtac tctgaacgat gatttggtga gcaggaattt ccagcatcat accgataatg
    11221 gaaccttgtg gaatgaatat cttgcttcta tccattatcc tttgataacc cgcagtctgt
    11281 gtcaattctt gatggagacc aggtgcatca aatgtataaa ctgctgtgat ctgattttgc
    11341 aaactttgct caatttggct agcagcatag atagcgagat ttcctccctt ggaatgccca
    11401 gctagaataa ccttttgctt aggatgatgg gcaaaaaagt tctttaaata gcgaagggcg
    11461 tgcttttgag caggaatttc cttcatatag gtcaggtgga aatcttcctt ccagccaatg
    11521 atactgtcat ctgtcccacg aaagacaatc agataggtat cgaggctgac acgataagtc
    11581 atagccgcaa attgcttttg cagttcaggg tcgatgtcgt tgataaaatg ggagagtttg
    11641 caatttttga agcgcttgtg ttgagccaat tcatctaata attgaaggcg atttttgcta
    11701 gtaagcatgg tgggatctct tggaacctga ggtgctagat ctaaaagacg ttgaggaagt
    11761 gtggagacca gattatcaaa ggagaggtag gtgatttcta ttaaggttag aatgtctaac
    11821 tcattcaagg gaaggtcgta ataagaatca tatgcgacat ctttcagata gtcaaaaata
    11881 ttggccataa gagctccttt ctttttattt atcttagcaa atttccctca aattagctag
    11941 tagcattgcc tgtttgtact ggctaaaaac aggctatttc aaattcagtt tcagaccatc
    12001 tggcatggaa aaatctgtta taataataga aaaggagaag cgcatgcaca agattttatt
    12061 aatagaagat gatcaggtca ttcgtcaaca ggtcgggaaa atgctctctg aatggggatt
    12121 tgaagtggtc ctggtagaag actttatgga agttttgagt ctatttgttc agtcggaacc
    12181 tcatctggtc ctcatggata ttggtttgcc cttgtttaat ggttatcact ggtgtcagga
    12241 aatccgcaag atttccaagg tacctatcat gtttctttct tcgagagacc aggctatgga
    12301 tattgtcatg gcaatcaata tgggggcaga tgactttgtg accaagcctt ttgaccagca
    12361 ggttctttta gctaaggttc agggcttgtt gcgtcgttcc tatgagtttg ggcgtgatga
    12421 gagtttgctg gaatatgctg gtgttatcct caataccaaa tccatggatt tacattatca
    12481 agggcaagtc ttgaatttga ccaagaatga attccagatt ttacgcgtgt tatttgagca
    12541 tgcaggcaac atcgtagcac gtgacgacct gatgcgggaa ctttggaaca gtgacttttt
    12601 cattgatgat aataccctct ctgtcaatgt ggctcgtttg cgtaaaaagt tggaggagca
    12661 gggattggta ggatttatcg agaccaagaa aggaataggg tacggattga agcatgcttg
    12721 attggaaaca attttttcta gcctatctgc gctcccgtag tcgtcttttt atctatctgc
    12781 tttctttggc atttcttgtc ttactctttc agtttttatt tgccagtcta ggaatttact
    12841 tcctctactt tttcttcttg tgttgctttg taaccatctt atttttcact tgggacatat
    12901 tggtggaaac gcaggtctat cgccaggaac ttctctatgg agagagggaa gccaagtctc
    12961 ctttggaaat agctttagca gaaaaattag aagcgcgtga gatggaactc tatcagcaga
    13021 ggtcaaaagc agaaagaaaa ctgacggatt tgctggatta ctataccttg tgggtccatc
    13081 agataaagac ccccattgca gccagtcaac tcttagttgc agaagtggtc gaccgccaac
    13141 tgaagcagca gctagaacag gaaattttca aaatcgactc ctataccaac ctagttttac
    13201 agtacctgcg tttagaaagt ttccatgatg atttgctctt aaagcaggtt caaattgagg
    13261 acttggtcaa ggaaataatt cgtaaatatg ctcttttctt tattcaaaaa ggcttaaatg
    13321 tcaatctaca tgaccttgat aaagaaatcg tgacggataa aaagtggctg ctagtggtta
    13381 ttgagcaaat catctcaaac agtctcaagt acaccaagga aggtggtctg gagatttata
    13441 tggatgacca agagctttgt atcaaagata cgggaatcgg gataaaaaac agtgatgtcc
    13501 tccgagtatt tgaacgtggc ttttcaggat acaatggccg tttgacccag cagtcctctg
    13561 gacttggcct ttatctatct aagaaaattt ctgaagaact ggggcaccag attcgtatcg
    13621 agtctgaggt cggaaaagga acgacagtgc ggattcagtt tgctcaagtg aacttagtcc
    13681 ttgagtaaaa atagatgaga aaaagcctcc agattggagg cttttatgct agactcttat
    13741 ttctcaacgc gtgatttgtt ggcgatatca gctaggatag cttgaacaaa attatcaact
    13801 gagacagttt gtgtttcttt ttggccgtag cgacgaacgt tgactgtttc gtcttccatt
    13861 tctttgtctc caacaattaa ttggtaagga atcttgctgg tttgtgaagc acggatcttg
    13921 aactgcattt tttcattgcg ctcatctacg tctgcacgga caccgcggtc acggagtttc
    13981 ttggccactt cccaagcgta gtccacgtgt ttttcgttag atactgggat gagggttact
    14041 tggtgtggtg ccagccatgt tgggaaggcc cccttgtagt tctcaatcaa gatagctgtg
    14101 aagcgttcca tagttgagat aaccccacgg tggatcatga ctggacggtg atcttcgcca
    14161 tcagctccga tgtatttgag gtcgaagcgt tctggcaaca agaaatcaag ttggatagta
    14221 gaaagggttt cttcttttcc aagggcagtt ttaatctgga tatccaattt tggtccgtag
    14281 aaggctgctt caccttcggc ttcaaagtag tccacgccca tttcatcaag agctgcacga
    14341 agcatggttt gggcattttc ccacatctca tcgttatcaa agtacttatg agtatcttga
    14401 gggtcacgaa gagagaggcg gaagcggtag tcagtcaagt tgaagtcttc ataaacatcg
    14461 ataatcaact gaagggcacg ttggaattct tcttggattt gttctggagt aacgaatagg
    14521 tgaccgtcgt tgagtgacat ttcacgtaca cgttgaaggc cagtgagggc accagatttt
    14581 tcgtaacggt gcatcatacc gatttcagcg atacggattg gcaattcacg gtaagagtga
    14641 acatggtgtt tgaaaacttg gatgtggtgc ggacagttca ttggacgaag gacaaattct
    14701 tccccgtcac ccatgtccat ggttgggaac atgtcttctt ggtaatgatc ccagtgacca
    14761 gaagtcttgt aaagctcaac agaagcaagt ggtggagtgt agacgtgttg gtagccagaa
    14821 gccaactctt tgtttacgat gtagcgttcc aattcacgac ggatagtcgc accatttggc
    14881 aaccagaatg gcaaaccttg tcccacttct tgtgaaatca taaagaggtc aagctcttta
    14941 ccaagtttac ggtggtcacg ttccttagct tcttcacgca tttgaaggta gtttttcaag
    15001 tctttcttgt caaaccaagc tgtaccgtag atacgttgca tcatagcgtt gtcgctgttt
    15061 ccacgccagt acgcaccagc tacatggaga aggtggaaga tttggatacg acctgttgat
    15121 ggaacgtgag gtccacggca gaggtctaca tattcaccct gacgatagat agtcaaaccg
    15181 ccttcgtctt ctgagtgttc ttcaatcaat tccaacttgt aagggtcatt tttgaagatt
    15241 tcacgtgcct cgtctttagt cacttcttca cgaatagatg ggaagttttc tttgacgatt
    15301 ttttgcattt cttcttcgat acgaggaagg tcttcgttag agatttgacc agctgtgttg
    15361 tcagtatcgt agtagaaacc atcttcgatg gctggaccaa ctcccaagtg aatgtctggg
    15421 aaaagacgac gagctgcttg ggcgaacaag tgagctgctg agtgacgcaa gattggaagg
    15481 gcatcttcgt gatcaggtgt cacaatttcg atgcttccat cttcagtgat agcgcgagta
    15541 gtgtcgatga gtttgccgtt gaatttacca gccaaggctt ttttagctag ggaattgctg
    15601 ataaattggg caatttcaaa agttgttacg ccagattcga attcacgaat agcgccatct
    15661 gggaaagtaa tgttaatcat gatgttctcc ttttttatta tttcatagtt tctaagcatt
    15721 gattttttat cagcagacta cttccaagtc ctaacgttca attgcttttc tttatcagag
    15781 aggaagtttt ccatttccgt cagaatatct atatccaaac gttgagaaag ttctaatagt
    15841 caccagatat tttctgaaag tttttgttcc agtgtgtagg gtgtttcatc atagtagcgt
    15901 ccttgttttg tcatcaccag tcgttggaaa tttccaatat cattagataa agccaagagg
    15961 tcttcttcta ccgtccactt gaaatcatga tgcttaactt ccagttcgtg ataagcttgt
    16021 cggattgccc aactgtgctc cactaattct tgcaactcca taattagtat cctaataaaa
    16081 aaatgcgaca tcctcgaaag gacgtcgcaa cgtggttcca ccttcattta tgtacctcaa
    16141 aagagaggga cacctctgat tggctctaac gtggccaccg ttttatgttt tcataaaaaa
    16201 ctcaagagta gtatcagttt agacttccta tgcgtttcca gcaaccacgc actctctagt
    16261 agaaagggac taagtgactt gtctctatgg attattatag catgattgtg agatttttca
    16321 aggattttgt gaaagttttt tgtttttcat tttttgtaga atatgcttga tgctaatacc
    16381 agaatccatg cccaccagag caactgtttc atagattagc aagtaaatta gtataaactc
    16441 accaaaggtt atgatagtaa agtaaaagag cagtccgtta ccaactaacc atcaaagtga
    16501 gaaacggaaa cggtagctat agagtaacga tacaatcaag attaccagag gtgtaaagaa
    16561 gagaagattg tttatataga gtcccttgag tgtaaggggg tcagaaacaa ctagtaagaa
    16621 caactcataa agtggccaat aaaagaataa tatgaccaga tagtagggga gatgggaata
    16681 gaatctacgc atgataatca cctcgtttta aaaaaacaga caacttagat ctcttgctat
    16741 gattattata gcatatttaa aacaagaaag taaatttgtt gacaaagaag attttttttc
    16801 actagaatca tggttagttt tcttggtaga aatattttga taataattaa gcaagactat
    16861 tgacattgat tattaagagt gtcacaattg ctgtaactaa aataattaca acaagagagg
    16921 tataaaaaat gaaactagca gttattgcag caaatggaca agtggggaaa gcaattgttg
    16981 aagaagcagt caaacgagga catgaagtaa cggctattgt tcgttctgaa aacaaaagtc
    17041 aagctgagtc tattattaaa aaagatttat ttgagttgac aaaagatgat cttactggat
    17101 ttgatgctgt tatttcagcg tttggtgctt atacaccaga tacgctaccg ctccatagca
    17161 aatcaatcga actatttaat cagcttcttg ctggaacaca gacacgtttt ctagttgtag
    17221 ggggagcagg aagcctttat atagatgaaa caaaaacgac acgactttta gatacgcctg
    17281 attttcctga agaatttaag ccactagcaa aagctcaagc ggatgaattg gatcttcttc
    17341 gtacaaaaaa taatctcaac tggacttttg tcagtcctgc tgtagatttt attcccgatg
    17401 gtgaaaaaac aggaaactac attctagcag gagaaatttt tactactaat ggaaaaggaa
    17461 taagccagat tagttatgca gattatgcta ttggattggt agatgaactt gaaaaaggtc
    17521 atcatataaa agaacgtatc tctttacttg aaaaataata actatgagag tattaatgtt
    17581 tgacagaaaa tttaaatctt gataaaatag ggattgtcgt aaagacaaat aacttcttct
    17641 tggttacagg catgccaacc tgtcactcgg atgaagccaa ataaaaagga gaaacatcat
    17701 ggcaatctca aaagagaaaa aaaatgaaat cattgcacaa tatgcacgtc acgaaggtga
    17761 tacaggttca gtagaggttc aagttgctgt ccttacttgg gaaatcaacc accttaacga
    17821 acacatcaaa caacacaaaa aagaccacgc tacttaccgt ggattgatga aaaaaatcgg
    17881 tcgccgtcgt aacttgcttg catacttgcg taaaaacgac gttaaccgtt accgtgaatt
    17941 gatcaactct ctaggacttc gtcgctaatt caagatacaa aggccgtcaa aagcacaaag
    18001 caaaaatagg aaaattgacg aagaaacttc agtttctagg agattttatc ttttttgcca
    18061 agtgcttagg ccgtgttcaa ttgagcatat cttgataata aagctactct aaattgggta
    18121 gcttttttgc gttgtcacct tacctcgcta tactcaagta taatcttcgg ttacggttcc
    18181 tagcactgta aggtaaaata aactagcttt tctcccttcg gggagatttt ttttgtttca
    18241 ctgaaagttt cgtacgatct tcgcctcgtc gcctagtcta taaacgattt atctagcaag
    18301 aatcatgatg ctaagggcgt caaaaatccg tatgaaaata gggaaatgac acagtgttcg
    18361 atgagcacaa ggagtttcat ctttttcact aggattttag cccgggctaa aatcagctct
    18421 ctgacttcag agggcttttt tattgaggtt ttatcggtaa caattttgga gactacaaaa
    18481 acctcaaatg gtatcagcta attacaccat aaaggtgcgc agttactcgg cttttcaagc
    18541 cgagtagctg tatgcaagcc ccctagaaga gcccactctt tacaaagtaa agtatagtat
    18601 gttatacttt acatggaagt agtcactgaa ttccagttag aaattacttt gtaactacgt
    18661 tttgaggagg agtaaaatgc tttcctacgt tcgacattac ccactagcga tagctaaatt
    18721 aatgtgtctg tgctctcctg aaatctgctg atttattact gactaataca ggaggttttt
    18781 ttatgggaca gacaatcata tctgctattg gtgtttatat ttccaccagt atcgattatt
    18841 taattatttt aattatttta tttgcacagc tatcacagaa taaacagaaa tggcatattt
    18901 atgcggggca atatctaggc acaggcttac ttgtaggggc gagtttagtt gctgcttatg
    18961 tcgttaattt cgtgcctgaa gaatggatgg ttggattgct tggtttaatc cctatctatt
    19021 tagggattcg ctttgcaatt gttggagaag atgcggaaga agaagaggaa gaaattattg
    19081 aaagattaga acaaagcaag gcaaatcaac tgttttggac agttacattg ctgacaattg
    19141 cgtctggcgg agataattta ggtatctata taccttattt tgcttcgtta gattggtcac
    19201 agaccctcgt ggccttgctt gtgtttgtaa tcggcataat tatcttttgc gagattagtc
    19261 gggtgttatc ctctattccg ttaatattcg agacaattga aaaatacgag cgaatcattg
    19321 tgcccttagt attcattcta cttggactat acatcatgta tgaaaatggc acgatagaga
    19381 cttttctgat cgtgtagatt tttttgtttc actaggattt tagcccgagc tcaaatcagc
    19441 tctctgattt tcagagggct ttttcattga gtataaatgg acaatcttaa agttactcta
    19501 ttgtagagtg gcttttgatt tgattaggag attcttataa atatagtaaa atgaaacaaa
    19561 aacaggacaa atcgatcagg acagtcaaat cgatttctaa aaatgtttta agaagtagag
    19621 gtgtactatt ctagtttcaa tttgatatat aaatgaaaac cttagctgtt tggccaaggt
    19681 tcatttcttc tatgcaagtt cttctctttt cttatctggg ttccagatga agcttgcgat
    19741 aaagctaaag atgatggtta ggatagcgag gataatagca aggatgaggg cagggatgcg
    19801 gataaaccac cagagtggat ttggtttttt atcattgtgc cattgtttcg tttcttcgtc
    19861 cagacgttgg aattctgttt gaatacgatt ggcgactgtt tcaatgcctt catcattcat
    19921 tttcttgata tctgagatat cgattggatt tccaaagttc atatcgacac gttcacggct
    19981 aatcaagccc ttcaaagtca tgggaccggt gtaggtaacc ggcatgatac ggaccttggc
    20041 cattttggca atcagtgctg cgcccccctt gacatcgttt gagtggcggc taccacttgg
    20101 aaacatgatg agagagcggt cacttttttt gagaacgttg ataggatatt tgatggctga
    20161 ggcgctggga ttttcacggt cgatgggaaa ggcgccacac atacgaatcc accaaccaaa
    20221 gatacggttg gtaaagagtt ctttttttgc cataaagatg aactgttttg gcttggtcgc
    20281 aaaggccata taaacaggat cccaccaggt acggtgaggc gcaactaaaa tataattttc
    20341 atcttgatta ggaattttat cagtattatg atagtgagca ttgccattga tggaccataa
    20401 gagcaataca actaatccac gcaaataagt ataaaacatg cgatctcctt cgattgtttt
    20461 cttgttatta ttatacctta tcaaaggagg gctggcaaac ttttcccttg actagatgca
    20521 tatttgggat gaaattagaa ttctgttaaa aaaaatgata taatagaatt tatggataaa
    20581 aataagatta tgggattaac ccaaagagaa gtcaaggaaa gacaggctga gggtttggtc
    20641 aatgacttta ccgcatcagc cagtaccagc acttggcaaa tcgttaaacg aaatgtcttt
    20701 acccttttta acgctttgaa ctttgccatt gctttggccc ttgcctttgt gcaggcttgg
    20761 agcaatctgg tcttctttgc tgttatctgc tttaacgctt tttctgggat tgtgaccgag
    20821 ctacgagcca aacacatggt ggacaagctc aatctcatga ccaaggaaaa ggtcaaaacc
    20881 atccgtgatg gtcaggaagt tgctcttaat cctgaagaat tagtgctagg agatgtcatt
    20941 cgtttgtctg caggagagca gattcctagt gatgccttgg ttttggaagg ctttgcggaa
    21001 gtcaatgaag ccatgttaac gggagaaagt gatttggtgc aaaaggaagt tgacggctta
    21061 cttttgtcag gaagtttcct agccagtggg tcagttttat ctcaagttca ccatgtcggt
    21121 gcagacaact atgctgccaa actcatgctt gaggctaaga ccgttaaacc catcaactcc
    21181 cgtatcatga aatcgctgga caagttggct ggttttactg ggaagattat cattcccttt
    21241 ggtctggctc tcttgctgga agccttgctt ttaaaaggcc tgcctctcaa gtcatccgtt
    21301 gtaaactcgt cgacagctct tttgggaatg ttgcctaagg gaattgccct tttgaccatt
    21361 acttcgctct tgactgcagt gattaagttg ggcttgaaaa aggtcttggt gcaggagatg
    21421 tactctgttg agaccttggc gcgcgtggat atgctctgtc tggacaagac gggcaccatc
    21481 acccaaggaa agatgcaggt ggaggctgtt cttccactga cggaaactta cggtgaagag
    21541 gctattgcca gcatcttgac tagctacatg gcccatagtg aggataagaa tccaactgcc
    21601 caagccattc gccagcgttt tgtgggagat gttgcttatc ctatgatttc caatcttccc
    21661 ttctccagtg accgcaagtg gggggctatg gagttagaag gcttggggac agttttctta
    21721 ggggcacctg agatgttgct ggattctgag gtcccagaag ccagagaggc cttggagaga
    21781 ggttcacgtg tcttggtctt agctctcagt caggagaaat tagaccatca caaaccacag
    21841 aaaccatctg atattcaggc tctagccttg ctggaaatct tggatcctat tcgagaggga
    21901 gcggcagaga cgctgaacta tctccgttct caggaagtgg ggctcaagat tatctctggt
    21961 gacaatccag ttacggtatc cagcattgcc cagaaggctg gttttgcgga ctatcacagc
    22021 tatgtcgatt gttcaaaaat cacggatgag gaattgattg ctctggcgga ggagacagcg
    22081 attttcggac gtgtttcccc tcatcaaaag aaactcatta tccaaacgct gaaaaaagcg
    22141 ggtcatacaa cagctatgac tggggatgga gttaatgata tcctggctct tcgtgaggcg
    22201 gattgttcta tcgtaatggc ggagggagac ccggcaaccc gtcagattgc caacctggtt
    22261 ctcttgaact cagactttaa tgatgttcct gagattcttt ttgaaggtcg tcgtgtggtc
    22321 aataacattg cccacatcgc tccgattttc ttgataaaga ccatctactc attcttgctc
    22381 gcagttatct gtatcgccag tgctttacta ggtcggtctg aatggatctt gattttcccc
    22441 ttcattccaa tccagattac catgattgac cagtttgtgg aaggtttccc accattcgtt
    22501 ttgacttttg agcgaaatat caaacctgtc gagccaaact tcctcagaag atccatgctt
    22561 cgtgccctac caagtgccct catggtcgtg tttagcgttc tttttgtgaa aatctttgga
    22621 acgagtcaag gttggtctga gttagaaatc tcaactctcc tctattatct cttgggggca
    22681 attggtttct tatccgtatt tagagcctgc atgccattca ctctatggcg agtcctattg
    22741 attgtctggt cagtaggagg cttcctagcc acagctctct tcccaagaat tcaaaaactg
    22801 cttgaaattg caaccttaac aggacaaaca ttacctgttt atggtgtcat gatgttggtc
    22861 tttaccgtga ttttcatcct gaccagtcgt taccaagaga aaaaataaag aaagactgca
    22921 atctgtggat tgcagttttt tatacgatga atctatcgta gatctgatgt gcaaggccta
    22981 cgtgtctatt atctatcggt gaacccaaga gtgattctca agcctgcttg gattgaggta
    23041 atagattcaa atatctgtag ttagactatt tgaagtttga tgtaagaaag agaaagcgac
    23101 agattgaagt aattttagct ctcttctatt gctagaacaa atggtcggat aggttggtag
    23161 tttgaaaatg aagatgctat ctattgttaa atggaacata gtgttattta ttagaaaatc
    23221 gtttggttta tttattatca aatacgaaaa gcaacttaaa tatttcaact aaaatagatg
    23281 ttatgaagaa aaggtaaaat gattttggca tagtgaggtt ctgttctatt tgatatcata
    23341 tttttgataa aaacaaaaat gtccattgca aaggacaaaa tgcgaagtat attatttttt
    23401 gaaagcgata taatggaatc ataaaggagg tgtatcgtgt ctagaaaaca agaacaaatg
    23461 gaaacgttgt tgctcctttt gcgagatagt aaggattata tatctgctaa agtattggga
    23521 gaaaaattaa attgctctga taaaacggtt tatcgccttg tcaaggtaat caacaaagat
    23581 tgtccggtag aagcattcat tttatctgaa aaaggcagag gtttcaaatt aaatccaaga
    23641 agttccctcg tggacgttga tgggaatttt acagaggctt ttgatcctga agtaaggcgt
    23701 gaaaaattac tagaacgtct cttgttgact gctcctaagc cacattctat ttatgattta
    23761 ggagaggaat tctacgtaag cgagtcagta gtactaaaag atcgtcagat attacaagag
    23821 agtctagcaa tttatgggtt agatttaaaa atgagacaac gaaagctttt tattgatggg
    23881 gatgaggctc aaattcgttc agccattcta aatctactgc caatgtttaa tcagttggat
    23941 ttagagcaaa ttacacagaa taaggttcag cctcttgacg gagaacttgc tcacttttgt
    24001 ttgggattac tgattacact tgagagagaa ttgggggtaa acattcccta tccatataat
    24061 ataaatattt tctctcacct gtatattttt atcagtagga atcgtcgtag tactagtatt
    24121 catgttgtag caccttcaaa acctactatt gttgatgaga aaatttacag tgtctgtcaa
    24181 aaaattattc aagaaattga acaatatttt aagatgaagg ttgatgcagt tgagattgac
    24241 tatctttatc aatacgttgt atcttcgaga ttgcaaaaac cattttcttc cgggaagctt
    24301 cctttttctc agcgagtttt agatgtcact cattactatt ttagccgtat gtgtatggac
    24361 aatagagaga ttgaaacgac agatcctgac tttgttgact tggcgagtca tatcagtccc
    24421 ttactgagga gattagataa tagagtacag attaagaata gtcttttatc acaaattctt
    24481 ttaacctatc ctaatctggt taaagagtta acaactattt ctaaagaagt gagtctagta
    24541 tttggttttg cgtccttgag tctggacgag attggttttc tagtcttata ttttgcacgg
    24601 tttcaagaaa agcgagcacg tcctctaaaa acagtagtga tgtgtacatc aggtgtcgga
    24661 acttcagagc ttttacgagc acgattagaa aagcaatttt ctgaattgga tattattgat
    24721 gtagttgctt atcatcaatt agatgagctg ataaatctat atccagattt agatttcatt
    24781 gtgacgacgg tagctttgca ggaaccagca agtgtcccgt ttgtcctagt tagtgctttt
    24841 ctaaccgagg gtgataaaca acgtcttcaa gcaaaaattc aggagataaa ctatgaataa
    24901 tctttcgctt gtccttatgg atatatctgt tcaaaatcgt caagaagcct acaaagaatt
    24961 agcaaatcaa atcagccttc ttgtttctga agatacagaa aaaatagaag agcttctata
    25021 ttaccgtgag agacagggaa gtatagaggt tgctaaaggt gttcttctac cacattgtga
    25081 aggaaacttt caacatcatg tcttagtgat tactagatta aaaccaccta tcagagaatg
    25141 gtcgaaggat atccagtgtg ttgaccttat tatcggtttg gccattgcag tatcacagga
    25201 caagtcatgt attaaaacat tgatgagaag actagcagat gaatcattca taaatcaatt
    25261 aaaacagtta acaaaagaag aattacggga gataatatat ggaaatcaaa gatattctta
    25321 atgtgagtct gatccagacg gatttacaga tgcagagcaa agaagaggtt tttgaggcat
    25381 tagctcaact attggttgag acgggttatg tgtctgatag agaccaattt atcgaaggtc
    25441 tttatcagag agaggcagaa ggacagactg gtattgggaa ttatattgct attccccata
    25501 gcaagagttc tgctgtggag aaggcggggg tagtcatagc tataaatcac aatgagattc
    25561 cttgggagac cattgatggg aaaggggtca aagtaattgt actctttgca gttggtgatg
    25621 atacagaagc tgctagggag catttgaaga ccttatcact ctttgctaga aaacttggta
    25681 atgacgaagt tgttgccaaa ttagttcggg ctcagacatc tgatgatgtg attgcagctt
    25741 tttgttaata agaaaaaatt ttggagggta tccgtatgaa aattgttggt gttgcagctt
    25801 gtactgtggg aattgcccac acttatattg cacaggaaaa attagagaat gccgcaaagg
    25861 tagctggaca tgtgattcat gttgagactc aggggacaat aggggtagaa aatgaattga
    25921 gtcaagagca gattgatgca gcggatgtag ttattttagc agttgatgtt aagatttctg
    25981 gtatggaacg ctttgagggt aaaaagatta tcaaggttcc aacagaagtg gcagtcaaat
    26041 ctcccaataa actgattgct aaagctgttg agattgttac gaaataactg aaaatattta
    26101 aggagaaaat atatgttgaa acacttaaac ttaaaaggtc acttattgac agccatttcc
    26161 tatatgattc caattgtttg tggcgcagga ttcttagttg ccattggttt agcaatgggg
    26221 ggtggtgttc ctgacgctct tgtagcagga aaattcacta tctgggatgc tttagcaact
    26281 atgggtggta aagcccttgg tctcttgcca gttgttattg ctacaggttt gtcttactcg
    26341 attgctggta agccagggat tgcaccaggt tttgttgttg gtctaattgc caattctgtt
    26401 ggttcagggt ttatcggtgg tatcttggga ggttatatag ctggtttctt ggttcaagcg
    26461 attattaaaa aggtcaaagt accaaactgg attaaaggtt taatgccaac cttgattatt
    26521 ccttttgtag cctctttggt aagtagtttg attatgattt atattattgg agcgcctatc
    26581 gcagccttta ccaactggtt gacgagctta ttacaaagct tgggaagtgc ttcaaatggt
    26641 ttgatggggg cagttattgg agttctcagt gctgttgact ttggtggccc acttaataaa
    26701 acagtctatg cgtttgtgtt gactttacag gctgaaggtg tgaaagaacc attgactgct
    26761 ttgcaattgg tgaatactgc tacaccagtt ggatttggat tggcctattt tatcgcgaaa
    26821 ttactcaaaa aaaatatcta tactcaagag gaaatcgaaa cattgaaatc ggctgttcct
    26881 atggggattg tcaatattgt tgaaggtgta attccgattg ttatgaataa cttggttcca
    26941 ggtctcattg caacaggtat cggtggtgct gttggtggtg ctgtttcttt gacaatgggt
    27001 gctgattctg ccgtgccatt tggtggagtg cttatgttac caaccatgac tcgtccagta
    27061 gctggtattt gtgctttgtt agccaacatt gtagtcacag gacttgtcta cgcgattttg
    27121 aaaaaaccaa taaaacatac agaaccagtt atgactgttg aagaagagat tgatttgtca
    27181 gatattgaaa ttttgtaaga gggtaacgat gtcaagaatt gaattttcac catctttgat
    27241 gaccatggat ttggacaaat tcaaagagca gattactttt ttgaatgata aagtagcatc
    27301 ttatcatatc gatattatgg atggccattt tgttcccaat attaccttgt ctccttggtt
    27361 cattcaagaa gttcaaaaaa ttagtgacac acctttatca gttcatctga tggtcacaga
    27421 cccaaccttt tgggtagatc aagttctcga tttacaatgt gagtatattt gtattcatgc
    27481 tgaagttctg aatggtcttg cttttcgttt gattgataaa attcatgatg caggtctaaa
    27541 ggctggtgtt gtccttaatc ctgaaacacc tgtttctaca atctttccct acattgattt
    27601 acttgacaaa gtaactatta tgactgtaga tccaggtttt gcaggacaac gctttttgga
    27661 gtctaccttg tataaaatcc aagaactccg tcagcttaga gttcagaatg gttatcacta
    27721 catcattgag atggatggtt cttcgagtcg taagactttc aaacaaattg atgtggcagg
    27781 accagatatt tatgttatag gtcgcagtgg attatttggt ttggatgacg atattgccaa
    27841 agcctgggat atctgttcta gagattacga agaaatgacc ggaaaaacaa tgccaatcaa
    27901 ataatggttt gagaagaaat ttattagtta ggaggaatat atgtcactac aatcagttaa
    27961 cgccattcgt tttcttggcg tagatgctat taacaaatct aattctggtc acccgggaat
    28021 cgtcatgggt gctgcgccaa tggcttatag cctatttaca aagcacctta gaattacacc
    28081 tgagcagcca aactggatta accgagatcg ctttatcttg tctgcgggtc atggatcaat
    28141 gctactgtat gctctcttgc atttaacagg gtataacgat gtatccatgg acgagattaa
    28201 aaatttccgg caatggggat ctaagacacc tggtcatcct gaagtgacgc atacgtctgg
    28261 tgtggatgcg acatctggtc cgcttggtca ggggatttct actgccgttg gtttcgccca
    28321 agcagagcgt tttttagctg ctaagtacaa caaagatggt ttccctattt ttgaccatta
    28381 tacttatgtt atcgctggag acggtgactt catggaagga gtgtctgcgg aggcggcttc
    28441 ttatgcaggt catcaagctt tagataagct tatcgtcctc tacgactcca acgacatctg
    28501 cttggatggt gagaccaaag atactttctc tgaaaatgtt cgcgcccgtt acgatgctta
    28561 tggttggcat acagttctgg tagaagatgg aacagattta gcagcaattt ctacagcaat
    28621 tgagacggcc aagttttctg gtaagccgag tttgattgaa gtgaaaacgg taattggtta
    28681 cggctcaccc aataaaagtg gtacaaatgc tgttcatggt gcaccactag gagcagaaga
    28741 aacaggagca actcgtaagt ttttgggatg ggattacgat ccatttgaag taccagagga
    28801 agtatattct gatttcaaga caaatgtagc ggatcgtggt caggaggcat acgatgcttg
    28861 ggctagtttg gtgtctgatt acaaggttgc ttatcccgaa gttgctagtg agattgacgc
    28921 tattgtagct ggaaaatccc ctgtaaccat tactgaaaaa gacttccctg tctatgagaa
    28981 tggcttctct caagcaactc gtaattcgtc ccaagatgct attaatacag cagcagcagt
    29041 tttaccaacc ttcttaggtg gatcggcaga cttagctcac tccaacatga cctacatcaa
    29101 ggcagatggc ttacaagata aatataatcc attaaaccgc aatattcagt ttggggtacg
    29161 tgaatttgcc atgggaacaa tcctcaatgg aatggctctt cattgtggtt tacgagttta
    29221 tggcggaacc ttctttgttt tctctgacta cgtcaaagct gctattcggc tatcagccat
    29281 tcaggagttg cctgtaactt atgtctttac ccatgattca attgccgttg gtgaagatgg
    29341 tccaactcat gaaccagttg aacatttggc aggtttacgc tcaatgccaa acttgactgt
    29401 tatccgtcca gcggatgccc gtgaaactca agcggcttgg catcatgcct tgaccagtac
    29461 caccactcca actgtcattg tcttaacccg tcaaaacttg gtagttgaag aagggacaga
    29521 ctttggtaag gtcgctaaag gagcctacgt cgtgtatgat accccgggat ttgatactat
    29581 tatcattgct acaggatctg aggtcaatct agctatcaaa gctgctaagg aattggtttt
    29641 acaaggtggt aaagtacgtg tggtatctat gccctcaacc gaactatttg atgctcaaga
    29701 tgctacctac aaggaagaca ttttaccatc taagactcgt cgtcgtgtgg ccattgaaat
    29761 ggcagcgacc caaagttggt acaagtatgt tggtttggat ggcgcggtca tcggtattga
    29821 catcttcggt gcgtctgccc cagctcagac tgtgattgat aattatggat ttacggtaga
    29881 gaatatcgtt gctcaagtta agtccctata gaaaccaatt acaatgaaga tacagctgtt
    29941 gtcagactag cagatgtagt gatagacact aatcagatga ttggttattt aaaaactata
    30001 atgagaatgt agtaatttat ctacgaaagt tatagtagat agtataccct gaaacggttg
    30061 cgaagtacgc taatcacttt gctactgatc tagatagttt ctttaatcaa taaacacagc
    30121 atccacagat tgacttagga tattgtaagt tttttgaaag ctagagagaa ggtctctaaa
    30181 attaaaaaac tcatagtata ggatgttgaa atgatgaact gc
//
LOCUS       DAVDWM010000023        28280 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVDWM010000023 DAVDWM010000000
VERSION     DAVDWM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.023"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            148..6849
                     /locus_tag="VUW49_001213"
     CDS             148..6849
                     /locus_tag="VUW49_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7981135.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSIMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKLEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIANTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLVTVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLVTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7097..7953)
                     /locus_tag="VUW49_001214"
                     /pseudo
     CDS             complement(7097..7953)
                     /locus_tag="VUW49_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8164..8523)
                     /locus_tag="VUW49_001215"
     CDS             complement(8164..8523)
                     /locus_tag="VUW49_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET7981136.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8615..9571
                     /locus_tag="VUW49_001216"
                     /pseudo
     CDS             8615..9571
                     /locus_tag="VUW49_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9555..10112
                     /locus_tag="VUW49_001217"
     CDS             9555..10112
                     /locus_tag="VUW49_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7981137.1"
                     /translation="MKRLLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10105..10359
                     /locus_tag="VUW49_001218"
     CDS             10105..10359
                     /locus_tag="VUW49_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981138.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10371..12425
                     /locus_tag="VUW49_001219"
     CDS             10371..12425
                     /locus_tag="VUW49_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7981139.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFIQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12604..13464
                     /locus_tag="VUW49_001220"
     CDS             12604..13464
                     /locus_tag="VUW49_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7981140.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13675..14385
                     /gene="ccdA2"
                     /locus_tag="VUW49_001221"
     CDS             13675..14385
                     /gene="ccdA2"
                     /locus_tag="VUW49_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET7981141.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14405..14971
                     /locus_tag="VUW49_001222"
     CDS             14405..14971
                     /locus_tag="VUW49_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET7981142.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14982..16094
                     /gene="msrB"
                     /locus_tag="VUW49_001223"
     CDS             14982..16094
                     /gene="msrB"
                     /locus_tag="VUW49_001223"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7981143.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16362..17099
                     /locus_tag="VUW49_001224"
     CDS             16362..17099
                     /locus_tag="VUW49_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981144.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17096..18787
                     /locus_tag="VUW49_001225"
     CDS             17096..18787
                     /locus_tag="VUW49_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7981145.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            18903..19466
                     /locus_tag="VUW49_001226"
     CDS             18903..19466
                     /locus_tag="VUW49_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET7981146.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            19488..25799
                     /locus_tag="VUW49_001227"
     CDS             19488..25799
                     /locus_tag="VUW49_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HET7981147.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEQAPVEPEKQPEAPEEEKVVEETPKQEESTPDTKAEETVEPKEE
                     TVNQSIEQPKVEQAGEPVAPREDEQAPTAPVEPREDEQAPTAPVEPEKQPEVPEEEKA
                     VEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYFTASYNALGPVLETAKGV
                     YASEPVKQPEVNSETNKLKTAIDALNVDKTELNNTIADLKTKTKEHHSDTSWQNLQTE
                     VTEAEKVAANTDAKQSEVDSETASLKTAISGLNTDKAELENQLKIAQEKTETDFSMGS
                     WTVLSTAQNKAQEVKDNGTATQDQINEAEQNLKTALAGLTVDKSQLESKLTDTASKVS
                     TDYSKTSWEKMTVAKETATVVKENQEAKQSQIDESAKALETALADLSVDKTALGSAID
                     TATKKNKENYTNQTWAELETVLTAAKSVNTNESKQSEVNEAVEKLTATIEKLVELSEK
                     PILTLTSTDKKILEREAVAKYTLTSTKAKIKSITAELKKGDTVIHTVILAGDNLKEAA
                     LSAEFNNLEYYKEYTLSTKIVYDRGNGDVTETLENQNIQLDLKKIEIKNINQTSLISV
                     DADGNESDKSLLSEKPTDISSYYLKVTTHDNKVTRLAIDTIEEVEEGGKTLFKVTATA
                     PDLIQRNSENKLSETYTHYFEKQIPKVDNVYYNFNELITDMQKNPNGIFKLGADLNAA
                     NVKPKGKSYVTTKFRGELLSADGGHYTIHNIERPLLADAEGANIHDINLANVNINIAW
                     ADKVAPLGNMFKKSSIRNIKVIGTITGNNDVTGMVNKLDESNMRNVAFIGKINSLGNK
                     GWWSGGLVSESWISNVDNAYLDADIVGNNSKFGGLIAKLDHGVDQFTVGKKGYLKNSF
                     VKGTMKLTHHGESGGLIHNNFNWGVVEDTISMMKVTNGEIMYGSRDLNGDDQYFGLDN
                     FKRTFYVNGVSEGLMSYKKSNNIQGISKEQALEKFAKMGITAHEFTITEPIVNKLNGI
                     KPKADTYKDTQDYKADRELAYRNIEKFQPFYNKEWIVEQGNRLATGSSLMTKEVLSVT
                     GMKDGQFVTDLSEIDKIMIHYADGTKEELAVTAKTDSKVAQVKGYDVAGQNIIYTPNM
                     VMKNRNQLASGIKEKLASVTLLSDEVRSLMDQRDKPWKNTPDKKTEYIKGLYFEESFE
                     EVKGNLEKFVSKILENEDHQLNGGEVVERALLKKVEDNKAKIMMGLTYLNRYYDIKYG
                     DLSIKDIMMFKPDFYGKTPSVLDRLIQIGSREHFLKGDRTQDAYKEVIAGATGKGDLR
                     SFLDYNMRLFTEDKDLNDWFIHSAKNVYVVEPETSTEAFKDKRHRVFDGLNNDIHGRM
                     ILPLLNLKKAHIFMISTYNTLAYSSFERYGKNTEEARESLKPKIISVAKAQQRYLDFW
                     SRLALPSVRDKLLKSQNMVPTPVWDSQWYDGIPDANRQGYGRGGAVVSPIRELFGPTD
                     RWHQVNGAMGAMAKIYERPWKDEQVFFMVTNMLEDFGISAFTHETTHVNDRMAYLGGW
                     RHRPGTNVEAFAQGMLQSPSEIGHQGEYGALGLNMAYVRPNNGNQWYNPDPEKLQTRE
                     QIDRYMKNYNDALMMLDHLEADAVIPKVQDNISRWFKKMDRQYRSGNSGNQFDRVREL
                     NEDEKNNLVLNTIDDLVTNNLITKHGAPSDRIYNPEDFDSAYVNINMMTGIYGGNTST
                     GAPGAASFKHNTFRMWGYFGYENGFVGYASSKYQDAANKENNKMLGDDFIIKKVSNNR
                     FSTLEEWKKAYYQEVKAKADKGFTAIEIDGKQITNYAELKVLFDKAVEADLAGGGTAK
                     TVELKSKVFKALLKNTDGFSGDLFTTPQA"
     gene            25869..27590
                     /gene="pabB"
                     /locus_tag="VUW49_001228"
     CDS             25869..27590
                     /gene="pabB"
                     /locus_tag="VUW49_001228"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET7981148.1"
                     /translation="MHRKTVIDFRVLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQDYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLNKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            27748..>28280
                     /locus_tag="VUW49_001229"
     CDS             27748..>28280
                     /locus_tag="VUW49_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET7981149.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDA"
BASE COUNT         8937 a         5156 c         6301 g         7886 t
ORIGIN      
        1 aatcatacat acggcaaggc gacactgacg aagtttgaat tggattttca cagagtgtaa
       61 attgttgaaa ctaggttgtc ataccatgta taccacttgg gctttagtag tagctaactg
      121 cactaaatat aatataagga gggttttatg gggaaaggcc attggaatcg gaaaagagtt
      181 tatagcattc gtaagtttgc tgtgggagct tgctcaataa tgattgggac ttgtgcagtt
      241 ctattaggag gaaatatagc tggagaatct gtagtttatg cggatgaaac acttattact
      301 catactgctg agaaacctaa agaggaaaaa atgatagtag aagaaaaggc tgataaagct
      361 ttggaaacta aaaatgtagt tgaaaggaca gaacaaagtg aacctagttc aactgaggct
      421 attgcatctg agaagaaaga agatgaagcc gtaactccaa aagaggaaaa agtgtctgct
      481 aaactggaag aaaaagctcc aaggatagaa tcacaagctt caagtcaaga aaaaccgctc
      541 aaggaagatg ctaaagctgt aacaaatgaa gaaatgaatc aaatgattga agacaggaaa
      601 gtggatttta atcaaaattg gtactttaaa ctcaatgcaa attctaagga agccattaaa
      661 cctgatgtag acgtatctac gtggaaaaaa ttagatttac cgtatgactg gagtatcttt
      721 aacgatttcg atcatgaatc tcctgcacaa aatgaaggtg gacagctcaa cggtggggaa
      781 gcttggtatc gcaagacttt caaactagat gaaaaagacc tcaagaaaaa tgttcgcctt
      841 acttttgatg gcgtctacat ggattctcaa gtttatgtca atggtcagtt agtggggcat
      901 tatccaaatg gttataacca gttctcatat gatatcacca aataccttca caaagatggt
      961 cgtgagaatg tgattgctgt ccatgcagtc aacaaacagc caagtagccg ttggtattca
     1021 ggaagtggta tctatcgtga tgtgacttta caagtgacag ataaggtgca tgttgagaaa
     1081 aatgggacaa ctattttaac accaaaactt gaagaacaac aacatggcaa ggttgaaact
     1141 catgtgacca gcaaaatcgc caatacggac gacaaagacc atgaacttgt agccgaatat
     1201 caaatcgttg aacgaggtgg tcatgctgta acaggcttag ttcgtacagc gagtcgtacc
     1261 ttaaaagcac atgaatcaac aagcctagat gcgattttag aagttgaaag accaaaactc
     1321 tggaccgttt taaatgacaa acctgccttg tacgaattga ttacgcgtgt ttaccgtgac
     1381 ggtcaattgg ttgatgctaa gaaggatttg tttggttacc gttactatca ctggactcca
     1441 aatgaaggtt tctctttgaa tggtgaacgt attaaattcc atggagtatc cttgcaccac
     1501 gaccatgggg cgcttggagc agaagaaaac tataaagcag aatatcgccg tctcaaacaa
     1561 atgaaggaga tgggagttaa ctccatccgt acaacccaca accctgctag tgagcaaacc
     1621 ttgcaaatcg cagcagaact aggtttactc gttcaggaag aggcttttga tacttggtat
     1681 ggtggcaaga aaccttatga ctatggacgt ttctttgaaa aagatgccac tcacccagaa
     1741 gctcgaaaag gtgaaaaatg gtctgatttt gacctacgta ccatggtcga aagaggcaaa
     1801 aacaaccctg ctatcttcat gtggtcaatt ggtaatgaaa taggtgaagc taatggtgat
     1861 gcccactctt tagtaactgt taaacgtttg gtcaaggtta tcaaggatgt tgataagact
     1921 cgctatgtta ccatgggagc agataaattc cgtttcggta atggtagcgg agggcatgag
     1981 aaaattgctg atgaactcga tgctgttgga tttaactatt ctgaagataa ttacaaagcc
     2041 cttagagcta agcatccaaa atggttgatt tatggatcag aaacatcttc agctacccgt
     2101 acacgtggaa gttactatcg ccctgaacgt gaattgaaac atagcaatgg acctgagcgt
     2161 aattatgaac agtctgacta tgggaatgat cgtgtgggtt gggggaaaac agcaaccgct
     2221 tcatggactt ttgaccgtga caacgctggc tatgctggac agtttatctg gacaggtacg
     2281 gactatattg gtgaacctac accatggcac aaccaaaatc aaactcctgt taagagctct
     2341 tactttggta tcgtagatac agccggcatt ccaaaacatg acttctatct ctaccaaagc
     2401 caatgggttt ctgttaagaa gaaaccgatg gtacaccttc ttcctcactg gaactgggaa
     2461 aacaaagaat tagcatccaa agtagctgac tcagaaggta agattccagt tcgtgcttat
     2521 tcgaatgctt ctagtgtaga attgttcttg aatggaaaat ctcttggtct taagactttc
     2581 aataaaaaac aaaccagcga tgggcggact taccaagaag gtgcaaatgc taatgaactt
     2641 tatcttgaat ggaaagttgc ctatcaacca ggtaccttgg aagcaattgc tcgtgatgaa
     2701 tctggcaagg aaattgctcg agataagatt acgactgctg gtaagccagc ggcagttcgt
     2761 cttattaagg aagaccatgc gattgcagca gatggaaaag acttgactta catctactat
     2821 gaaattgttg acagccaggg gaatgtggtt ccaactgcta ataatctggt tcgcttccaa
     2881 ttgcatggtc aaggtcaact ggtcggtgta gataacggag aacaagccag ccgtgaacgc
     2941 tataaggcgc aagcagatgg ttcttggatt cgtaaagcat ttaatggtaa aggtgttgcc
     3001 attgtcaaat caactgaaca agcagggaaa ttcaccctta ctgcccactc tgatctcttg
     3061 aaatcgaacc aagtcactgt ctttactggt aagaaagaag gacaagagaa gactgttttg
     3121 gtgacagaag tgccaaaagt acagaccatt attggagagg cacctgaaat gcctaccact
     3181 gttccgtttg tatacagtga tggtagccgt gcagaacgtc ctgtaacctg gtcttcagta
     3241 gatgtgagca agcctggtat tgtaacggtg aaaggtatgg ctgacggacg agaagtagaa
     3301 gctcgtgtag aagtgattgc tcttaaatca gagctaccag ttgtgaaacg tattgctcca
     3361 aatactgact tgaattctgt agacaaatct gtttcttatg ttttgactga tggaagtgta
     3421 caagagtacg aagtagatag ctgggagatt acggaagtag ataaagccaa actttcagta
     3481 gctggatcac gtattcaaat gactggtcag ttagctggag aaactattca tgcaaccctt
     3541 gtggtagaag aaggaaatgc tgcagcacct gtagtgccaa ctgttactgt tggaggtgaa
     3601 gcagtaacag gtcttactag tcgacaacca atgcaatatc gtactctatc ttatggtgcc
     3661 caattgccag aagtcacagc aagtgctgaa aatgctgatg tgacagttct tcaagcaagc
     3721 gcagcaaacg gcatgcgtgc aagcatattt gttcagccta aagatggtgg ccctcttcaa
     3781 acctatgcaa ttcaattcct agaagaagca ccaaaaattg ctcacttgag cttgcaagtg
     3841 gaaaaagctg acagtctcaa agaagaccaa actgtcaaat tgtcggttcg agctcactat
     3901 caagatggaa cgcaagctgt attaccagct gataaagtaa ccttctctac aagtggtgaa
     3961 ggggaagtcg caattcgtaa aggaatgctt gagttgcata agccaggagc agtcactctg
     4021 aacgctgaat atgagggagc taaaggccaa gttgaactca ctatccaagc caatactgag
     4081 aagaagattg cgcaatccat ccgtcctgta aatgtagtga cagatttgca tcaggaacca
     4141 agtcttccag caacagtaac agttgagtat gacaaaggtt tccctaaaac tcataaagtc
     4201 acttggcaag ctattccgaa agaaaaacta gactcctatc aaacatttga agtactaggt
     4261 aaagttgaag gaattgacct tgaagcgcgt gcaaaagtct ctgtagaagg tatcgtttca
     4321 gttgaagaag tcagtgtgac aactccaatc gcagaagcac cacaattacc agaaagcgtt
     4381 cggacatatg attcaaatgg tcacgtttca tcagctaagg ttgcatggga tgcgattcgt
     4441 ccagagcaat acgctaagga aggtgtcttt acagttaatg gtcgcttaga aggtacgcaa
     4501 ttaacaacta aacttcatgt tcgcgtatct gctcaaactg agcaaggtgc aaacatttct
     4561 gaccaatgga ccggttcaga attgccactt gcctttgctt cagattcaaa tccaagcgac
     4621 ccagtttcaa atgttaatga caagctcatt tcctacaata accaaccagc caatcgttgg
     4681 acaaactgga atcgtagtaa tccagaagct tcagtcggtg ttctgtttgg agattcaggt
     4741 atcttgagca aacgctccgt tgataatcta agtgtcggat tccacgaaga ccatggagtt
     4801 ggtgcaccga agtcttatgt gattgagtat tatgttggta agactgtccc aacagctcct
     4861 aaaaacccta gttttgttgg taatgaggac catgtcttta atgattctgc caactggaaa
     4921 ccagttacta atctaaaagc ccctgctcaa ctcaaggctg gagaaatgaa ccactttagc
     4981 tttgataaag ttgaaaccta tgctgttcgt attcgcatgg ttaaagcaga taacaagcgt
     5041 ggaacgtcta tcacagaggt acaaatcttt gcgaaacaag ttgcggcagc caaacaagga
     5101 caaacaagaa tccaagttga cggcaaagac ttagcaaact tcaaccctga tttgacagac
     5161 tactaccttg agtctgtaga tggaaaagtt ccggcagtca cagcaagtgt tagcaacaat
     5221 ggtctcgcta ccgtcgttcc aagcgttcgt gaaggtgagc cggttcgtgt catcgcgaaa
     5281 gctgaaaatg gcgacatctt aggagaatac cgtctgcact tcactaagga taagaactta
     5341 ctttctcata aaccagttgc tgcggttaaa caagctcgct tgctacaagt aggtcaagca
     5401 cttgaattgc cgactaaggt tccagtttac ttcacaggta aagacggcta cgaaacaaaa
     5461 gatttgtcag ttgaatggga agaagttcca gcagaaaatc tgacaaaagc aggtcaattt
     5521 accgttcgag gccatgtcct tggtagtgat cttgttgctg aggtcactgt acgagtgaca
     5581 gacaaactag gcgaagctct ttcagataac cctaactatg atgaaaacag taaccaggcc
     5641 tttgcttcag caaccaatga tattgacaaa aactctcatg accgcgttga ctatctcaat
     5701 gacggagatc attcagaaaa tcgtcgttgg acaaactggt ctccaacacc atcttctaat
     5761 ccagaagtat cagcgggtgt gatcttccgt gaaaatggta agattgtaga acggactgtt
     5821 gcgcaagcca aacttcactt ctttgcagat agtggtacgg atgcaccaac taaactcgtt
     5881 ttagaacgct atgtcggtcc agagtttgaa gtgccaacct actattcaaa ctaccaagcc
     5941 tacgacgcag accatccatt caacaatcca gaaaattggg aagctgtgcc ttatcgtgcg
     6001 gataaagaca tcgcagctgg tgatgaaatc aacgtaacat ttaaagctgt caaagccaaa
     6061 gctatgagat ggcgtatgga gcgtaaagca gataagagcg gtgttgcgat gattgagatg
     6121 accttccttg caccaagtga attgcctcaa gaaagcacgc aatcgaagat tcttgtagat
     6181 ggaaaagaac ttgctgattt cgctgaaaat cgtcaagact atcaaattac ctataaaggt
     6241 caacggccaa aagtctcagt tgaagaaaac aatcaagtag cttcaactgt ggtagatagt
     6301 ggagaagata gccttccagt acttgttcgc ctcgtttcag aaagtggaaa acaagtcaag
     6361 gaataccgta tccacttgac taaggaaaaa ccagtttctg agaagacagt tgctgctgta
     6421 caagaagatc ttccaaaact cgaatttgtt gaaaaagatt tggcctacaa gacagttgag
     6481 aaaaaagatt caacactgta tctaggtgaa actcgtgtag aacaagaagg aaaaactggt
     6541 aaagaacgta tctttacagc gattaatcct gatggaagta aggaagaaaa actccgtgaa
     6601 gtggtagaag ctccgacaga ccgcatcgtc ttggttggaa ccaaaccagt agctcaagaa
     6661 gctaaaaaac cacaagtgtc agaaaaagca gatacaaaac caattgattc aagtgaagct
     6721 agtcaaacta ataaagccca gttaccaaat acaggtagtg cggcaagcca agcagcagta
     6781 gcagcaggtt tagctcttct aggtttgagt gcaggattag tagttactaa aggtaaaaaa
     6841 gaagactaga aattttgata ccttctttat cattaaagac caaagaaaaa ctcttgtcct
     6901 aaatagaaga taaagagtct ggaacaaaat agttctcaac cacaaaaaag ctagatattt
     6961 ccaattgcgg aactctagct ttttaatttt gagtcgttct cttattgtat agtatattga
     7021 aactagaata gtacacttct acttctaaaa cattgttaga aatcaatttg actgtcctta
     7081 tcgatttgtc ctgttcttat ttcatttcac tatgttttag gaggttgttg gccataagta
     7141 ccaatcccat gtcaattctc acttgacgct tgcctctcag attacatctc ttgtaaccca
     7201 aacaagcctt tatctgccca aagacaggtt ccacatcaat cttgcgttga gcgaaaatct
     7261 gtctaccttg gggagataaa agcgcctgac attctttagc tttcaagttt tgatagcgtt
     7321 cgttcatata cagtcccttt tgagggactg attcaggttc atcagcgtag taaaccttga
     7381 tttcctgttg aaagtctgtc tgtgttttct ggtacttgat atggtgaaaa cgatagcacc
     7441 agccatcagg atgtgtgtag ctatcctcct tgtcattata gtgccaattc gctaagtttt
     7501 tagctgactg tttataccct ctcttttgtt tcttatcaaa catggcatat ttaatcagaa
     7561 ggggagtgat ttcttcttga aaatagactt gtatctgttc ttgaagtttg gcggagaatt
     7621 tctctgtcgt tttcttccac acaaaactat acttgttggc attggcttca atcttagttc
     7681 cgtcaataaa caaacaatct aaggtcacta actcttccat ttttcaattt ttcgaccaga
     7741 gaaaatccct tgtgaatagg caaatagaag agtagataca agcattttag ggtgataaga
     7801 cgggcgacca aaggcatgat agaaggcgtg gaaatgacaa tcctccaagg tattcaccac
     7861 tttttcaata gtaaaaacga gatggtcttg tggcaagaag gaactgattt ctagtggtaa
     7921 agttgtttga tttgtgttat agtgaatatg catgggatag cctttctaaa tggtttatta
     7981 agcaattcta ttttaccaag actatcgtaa ccatgcaaaa aagcaacggc aaatccgttg
     8041 ccttttttgg atataagaaa ctattgtccc agactctttt ctttgtataa aattgtatag
     8101 tgttattgtc tatactattt tgtagagaaa gcaaaaatcc ctagaaaatg cttagggatt
     8161 cgactatttc attaagaaca attcaacaat catttctata tacatgataa gggttaataa
     8221 gaatcctgca cgccagaaga atttgataaa tttaggatag taaaagctgc gttttttcaa
     8281 aaggaagaaa aagacgagaa taatggctag gatcgagagg gctagcccca gtctagggag
     8341 gaaattatgg gtaaaggttt tagctgtaat caagtaatac tcaaatacca agactggaaa
     8401 agccaaatcc gcaaagtttc gtcctagttt ttttaatcta aaaagtttgg ttaggataat
     8461 gcagacgact aaggtcagta tcaataataa aatagatgct aatttcatta aaatcatacc
     8521 catattgtat cataaaaaaa ggctaatgga aacgagaaac agaggaattt ataggaaagt
     8581 caggcttttg agattgtggg tgatttttgt tataatgaaa gttataaaat cttataatac
     8641 cttgaatgat tattatcgaa aactctttgg agaaaagact tttaaagtcc ctattgatgc
     8701 gggatttgac tgtcccaatc gtgatggaac tgtggctcat ggaggctgta ctttttgtac
     8761 ggtttccggt tctggagatg ccattgtggc accagatgcg cctatccgtg agcaatttta
     8821 taaggaaatt gactttattc atcgtaagtg gccagatgtt cagaagtatc tggtttattt
     8881 tcaaaatttt accaacaccc atgaaaaggt ggaagttatt cgagagcgtt atgaacaggc
     8941 tatcaatgag ccaggtgtgg taggaatcaa tattggaacg cgcccagact gtttaccaga
     9001 cgaaaccatc gaatatttgg ctgagttatc ggagtgcatg catgtgacgg ttgaattggg
     9061 cttgcaaact acttatgaag caacctctga cctgattaac cgtgcgcatt cttatgaatt
     9121 gtaggtggaa acggtcaagc gtttgagaaa gtatccaaaa attgagattg tttcccattt
     9181 gatcaatggt ttgcctggtg aaacccatga gatgatggtt gaaaatgtcc gccgttgtgt
     9241 cacggataac gatattcaag ggattaaact gcatttgctc catcttatga caaatactcg
     9301 tatgcaacga gattaccatg aggggcgttt acaattgatg agtcaggacg aatatgtcag
     9361 ggtcatctgt gaccaactgg aaattattcc caaacatatc gtcatccatc gaattacagg
     9421 agatgcgcct agggatatgc tgattggtcc tatgtggagc ctcaaaaaat gggaagtcct
     9481 caacagcatt gagatggaga tgcgacgtcg tggaagtgtt caaggatgca aggctgtaaa
     9541 acaggagttt gaaaatgaaa agactacttg agatggcaca tgattttttg gctgaggtcg
     9601 tgacaaaaga ggatgtcgta gtggatgcga ctatgggaaa tggtcatgac acgctttttt
     9661 tagccaagct agccaagcaa gtctatgcct ttgatattca gaagcaagcc ttggaaaaga
     9721 cccaagagcg tttgcatcag gctggcttga caaatgccca gttaatcttg caaggccatg
     9781 agacactgga ccagtttgtg acaaaagcta aggcagggat ttttaatctg ggctatttgc
     9841 cgtcagctga taagtctgtc atcacccgac cgcagacaac gattgaggca ttagaaaagc
     9901 tatgtggctt acttgtcaaa ggtggacgaa ttgctattat gatttactat ggtcatgaag
     9961 gaggcgacct cgagagagat gctgtcttgg attttgtgat ccagttgaac caacaagagt
    10021 acacagctgc catttaccga actttaaacc aagtcaacaa cccgccgttt ttagtgatga
    10081 ttgaaaaatt agagagatac agacatggat aaacaatacc tacacgaaaa gctggatgcc
    10141 atgcgccaga attttgttga atcaacccac cacgaacgag cggtgggcgt gctagaccaa
    10201 gcgcatatga gcaaaaaaat gcttaaaatc aagaaaaaat tagttgctct tgaaatggaa
    10261 cggtgccaga gaaaaattga gcacaaagac tgttccaaga ttgaccaaaa aatcaaagag
    10321 cagaaggaga tatttgaatc ctgttgtaaa aaagattaag gaggacgtgc gtggaattac
    10381 tgatttacct catcctattt ttactggttt tgattgtctc aagtacaacc aataagctcc
    10441 tacccttttt gcctctccct ttggtgcaga ttcttttggg aattgtgatt ggtctctttt
    10501 tacccaatac tgactttcac cttaatacgg agttgttttt ggcactggtt atcggaccct
    10561 tgcttttccg agaggctgaa gaagcagatg ttacggctat tttaaaacac tggcgaatca
    10621 ttgtttatct catatttcca gtgattttta tctcgaccct gagtttgggt ggcttggccc
    10681 atcttctttg gttcagcctt cccttggcag cttgcttggc tgttggggca gcccttggtc
    10741 ctacggactt ggtggccttt gcctctcttt cggagcgttt tagctttcct aagcgcgtgt
    10801 ccaatattct taagggcgaa ggactcttga atgatgcttc tggtttggtg gcttttcagg
    10861 tagctttgac agcttggaca actggagctt tttctctggg gcaagctagc agttcgctca
    10921 tcttttcaat cctaggcggt tttttaattg gatttttaac agccatgacc aaccgcttcc
    10981 tccatacctt cttgctaagt gtgcgcgcaa cggatattgc cagtgaactt ttattagaat
    11041 tgagtttgcc tctagtgacc ttctttctgg cagaagaagt ccatgtttca ggtattattg
    11101 ccgtcgtagt tgctggaatt ttaaaggcaa gtcgcttcaa gaaaatcacg ctcctcgaag
    11161 cccaagtgga tacggtgacc gagacggtct ggcatacagt gacctttatg ctcaacggtt
    11221 ctgtctttgt gattttaggg atggagttgg aaatgatagc agaacctatc ttgaccaatc
    11281 caatctataa tcctctactt ttattgctat ctctcatcgc ccttaccttt gtcctctttg
    11341 tcattcgttt tattatgatc tatggctatt atgcctatag aacccgacgc ctcaagaaaa
    11401 agctaaataa gtatatgaag gacatgtttc tcttgacctt ttcaggtgtt aagggaacgg
    11461 tgtcgattgc tacgattctc ttgataccaa gtaatctaga acaggagtat cctctcttgc
    11521 ttttccttgt tgcaggtgtg acgcttgtca gctttttaac aggtctcttg gtcttgcctc
    11581 atctttctga tgaagaggaa gaaagcaagg attatctcat gcatatcgcc attttgaatg
    11641 aagtaacgct agagttggaa aaagagttgg aagacaccag aaataaactt cccctctatg
    11701 cggctattga caattatcat ggacgtattg aaaatctcat tttaagccaa gaaaaccagg
    11761 atgatcaaga agattgggct gctttgaaac tcttgattct tagtattgaa agtgatggtt
    11821 tggagcaggc ctatgaagag gggaacatta gcaatcgtgc ttaccgagtt taccaacgtt
    11881 atctgaaaaa tatagaacaa ggaatcaatc gtaaacttgc ctcaagactg acctattatt
    11941 tccttgtttc tttgcggatt ttacgttttc tccttcatga agtttttact ctcggaaaga
    12001 ccttccgtag ctggaagaac aaggagcaaa gccgtctccg tgctcttgat tatgaccaaa
    12061 ttgcagagct ctatcttgcc aatacggaga tgattattga aagtttggaa aacctgaagg
    12121 gagtctacag acgctctttg attagtttta ttcaggagtc tcgtcttcga gaaacaacta
    12181 ttatcagcag tggtgccttt gtcgaacggg ttatcaatcg tgtcaaaccc aacaatatca
    12241 atgaaatgct gagaggctat tatctggagc gcaagttgat tttcgaatac gaagaaaaac
    12301 gattaattac aactaagtat gccaagaaat tacgacaaaa tgtaaataac ttagagaact
    12361 attccttgaa ggaagctgcc aataccctgc cgtatgatat ggtggaattg gtaagaagaa
    12421 attagttaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt ggattatata
    12481 tgtgactgac ttcgtcagtt tcatctacaa cctcaaagca gggctttgag caacctgcgg
    12541 ctagcttcct agtttgctat ttgattttca ttgagtataa gattataagt gaaggagtgt
    12601 gacatgaaaa agtggtggaa agagctgata gacaagcctt tattaaaagc ttttttgcat
    12661 tattatcaag catcagatag tgagttgacc agtgtcgcag tagcctacta ttggttgatt
    12721 tcgatcttcc ccctgctttt ggtggtggtc aatatcctcc cctattttca gattcctgtg
    12781 ggagaatttc tgggctttat gaaagacgtt ctgcccccaa gcctctatga aggtgtggaa
    12841 aaaatagcca gagaagtttt gacgcaacct tcaacaggtt tattgagttt ttctgtttta
    12901 tcggcgttat ggagtttttc aaaatctatg aatttcttgc aaaaagcttt taacaaggct
    12961 tatggcgtcg aaaagagtcg tggacttatt tcccatcaga tgctaagtct cctagtcagt
    13021 tttggcttgc agctcctctt tgcgtttgcc ttgtttttga tcttatttgg tcagatgatt
    13081 ttggctttac ttgctcatta ttggacaaaa gatggcatta tctatcaggc tttacaaggg
    13141 ttggcaggtc ctctgattta cgccctcctc tttgctatct tggtcatgct ttattatttt
    13201 cttcccaata tatctaacag gaaaattagc tataccctac caggcagtgc ctttgttctc
    13261 ttagtgattt taggtttact aactctgttc tctagttatc tcaattacta tgtccatcat
    13321 ttggtggatg tccgaatttt aggttccgtt cttcttgttg ttatgatgtt ttggtttatc
    13381 ttgattgcta aaattgttat cctaggcgcg gttatcaatg ccagtatgca gagtttgaaa
    13441 gatccggtct ttaaaaaaga ctaactattt atccattaca aaagcgcata gtatcaaaac
    13501 tttatagaac cttgatacta tgcgcttttg tgctttgacg gttttttgta tagtttttgt
    13561 ccaagatttc taaatcatct gattaaattc ctattttccc ctatcattgt ccctgttttt
    13621 tcgaaaatgg aaggagtata ataaatctat caataaaatg ataggaggaa ctttatgggt
    13681 catattttct tttttctaag tgtctttttg gcagggattc tatccttctt ttctccttgt
    13741 atcttacctt tgttaccggt ctatacagga gtgttactag atgataagga tggtgctcag
    13801 gcttctagcg gcaaattttc aatctcagtt actagtttat tacgaactct ggcctttata
    13861 gcaggaattt cctttatatt tattttgttg ggctatggag ctggtttttt aggcgatttg
    13921 ctttatgctt cttggttcca atatcttact ggggcaatta ttatccttct tggtttgcac
    13981 caaatggaga ttctacactt taaggggctt tataaggaaa agaggctaca actgcaagga
    14041 caggggcaaa atggtaaggg ctatagtcag gcatttttat tgggcttgac ctttagtttt
    14101 gcttggacgc cttgcgtggg gccggttctg gggtctgttt tggccttggc ggcttcaggt
    14161 ggttcaggag cttggcaggg agctggtctc atgttggtgt atacgctggg cttggcgcta
    14221 ccattcttgc ttctagctct gacctctagt tatgttttga aacatttccg aaaacttcat
    14281 ccctatctcg gaatcctcaa aaaagtgggt ggttttctca ttattgtgat gggactcttg
    14341 gttctgtttg gaaatgcttc aattttaagt caattatttg aataaaatgg aaaggaatat
    14401 caatatgaaa aaatggcaaa catgtgttct tggagcaggt tcgctccttt gtttgacggc
    14461 ttgttcaggc aagtccgtga ctagtgaaca ccaaacgaaa gatgaaatga agacggagca
    14521 gacagctagt aaaacaagcg cagctaaagg gaaagaggtg gctgattttg aattgatggg
    14581 agtagatggc aagacctacc gtttatctga ttacaagggc aagaaagtct atctcaaatt
    14641 ctgggcttct tggtgttcca tctgtctggc tagtcttcca gatacggatg agattgctaa
    14701 agaagctggt gatgactatg tggtcttgac agtagtgtca ccaggacata agggagagca
    14761 atctgaagcg gactttaaga attggtataa gggattggat tataaaaatc tcccagtcct
    14821 agttgaccca tcaggcaaac ttttggaaac ttatggtgtc cgttcttacc caacccaagc
    14881 ctttatagac aaagaaggca agctggtcaa aacacatcca ggattcatgg aaaaagatgc
    14941 aattttgcaa actttgaagg aattagccta ggaggtgtct tatgaatgat aagttaaaaa
    15001 tcttcttgtt gctaggagta ttttttctag ccataaccgg tttctatgtt ctattgatac
    15061 gaaatgcagg gcagacagat gcctcgcaaa ttgaaaaggc ggcagttagc caaggaggaa
    15121 aagcagtgaa aaaaacagaa attagtaaag acgcagactt gcacgaaatt tatctagctg
    15181 gaggttgttt ctggggagtg gaggaatatt tctcacgcgt tcccggggtg acggatgccg
    15241 tttcaggcta tgcaaatggt agaggagaaa caaccaagta cgaattgatt aaccaaacag
    15301 gtcatgcaga aaccgtccat gtcacctatg atgccaagca aatttctctc aaggaaatcc
    15361 tgcttcacta tttccgcatt atcaatccaa ccagcaaaaa taaacaagga aatgatgtgg
    15421 ggacccagta ccgtactggt gtttattaca cagatgacaa ggatttggaa gtgattaacc
    15481 aagtctttga tgaggtggct aagaaatacg atcaacctct agcagttgaa aaggaaaact
    15541 tgaagaattt tgtggtggct gaggattacc atcaagacta tctcaagaaa aatccaaatg
    15601 gctactgcca tatcaatgtt aatcaggcgg cctatcctgt cattgatgcc agcaaatatc
    15661 caaaaccaag tgatgaggaa ttgaaaaaga ccctgtcacc tgaggagtat gcagttaccc
    15721 aggaaaatca aacagaacga gctttctcaa accgttactg ggataaattt gaatccggta
    15781 tctatgtgga tatagcaact ggggaacctc tcttttcatc aaaagacaaa tttgagtctg
    15841 gttgtggctg gcctagtttt acccaaccca tcagtccaga tgttgtcacc tacaaggaag
    15901 ataagtccta caatatgacg cgtatggaag tgcggagccg agtaggagat tctcaccttg
    15961 ggcatgtctt tacggatggt ccacaggaca agggcggctt acgttactgt atcaatagcc
    16021 tctctatccg ctttattccc aaagaccaaa tggaagaaaa aggctacgct tatttactag
    16081 attatgttga ttaatactct tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt
    16141 atgtagggtt actgacttcg tcagttttat ctacaacctc aaagcggtac tttgagctga
    16201 cttcgtcagt tttatttaca acctcaaagc ggtactttga gaaacctgtg gctatcttcc
    16261 tagtttactt tttgattttc attgagtata agaggtcttt cctaagcagt tagaggagaa
    16321 ttttgctata ctgatactag taagtggtaa aggagcagat catgacctac acaatcttaa
    16381 tcgtagaaga tgaatatctg gtaagacaag gtttgactaa actggtcaat gtagcagcct
    16441 atgatatgga aatcatcggt caggctgaaa atggaaggca ggcttgggaa ttgatccaaa
    16501 agcaggtgcc agatatcatt ttaaccgata tcaacatgcc tcatctaaat ggcatccagt
    16561 tggccagtct ggtacgagaa acctatcctc aggttcattt ggtcttttta acaggttacg
    16621 atgattttga ttatgccttg tctgctgtca aactaggtgt ggacgactac ctactcaaac
    16681 ccttttctcg tcaggatatt gaggaaatgt tggggaaaat caaacaaaaa ctagacaagg
    16741 aagagaaaga agagcagtta caagatttat tgactaatag gtttgaagga aacatggccc
    16801 agaaaatcca gtctcatctg gctgatagcc aatttagttt aaagtcttta gccagtgact
    16861 taggttttag tccgacctat ctgagttcct tgattaagaa agagttgggc ttgccttttc
    16921 aggattatct ggtgagagaa cgtgttaaac aagccaagct cttgctttta actacagatt
    16981 tgaagattta tgagatcgca gagaaggttg gttttgaaga tatgaactat tttacccaac
    17041 gttttaagca gattgcaggt gtgacacctc gtcagtttaa gaagggagaa gaccgatgaa
    17101 gcgttcttct cttttagtta gaatggttat ttccatcttt ctggtctttc tcattctcct
    17161 agctctggtt ggaactttct actatcaatc aagttcttca gccattgagg ccaccattga
    17221 gggcaacagc caaacgacca tcagccagac tagccacttt attcagtctt atatcaaaaa
    17281 actagaaacc acctcgactg gtttgaccca gcagacggat gttctggcct atgctgagaa
    17341 tcccagtcaa gacaaggtcg agggaatccg agatttgttt ttgaccatct tgaagtcaga
    17401 taaggacttg aaaactgttg tgctggtgac caaatctggt caggtcattt ctacagatga
    17461 cagtgtgcag atgaaaactt cctctgatat gatggctgag gattggtacc aaaaggccat
    17521 tcatcaggga gctatgcctg ttttgactcc agctcgtaaa tcagatagtc agtgggtcat
    17581 ttctgtcact caagaacttg ttgatgcaaa gggagccaat cttggtgtgc ttcgtttgga
    17641 tatttcttat gaaactctgg aagcctatct caatcaactc cagttggggc agcagggctt
    17701 tgccttcatt atcaatgaaa accatgaatt tgtctaccat cctcaacaca cagtttatag
    17761 ttcgtctagc aaaatggagg ctatgaaacc ctacatcgag acaggtcagg gttatactcc
    17821 tggtcacaaa tcctacgtca gtcaagagaa gattgcagga actgattgga cggtacttgg
    17881 cgtgtcatca ttggaaaagt tagaccaggt tcggagtcag ctcttgtgga ccttgcttgg
    17941 ggccagtgtc acatctcttc ttgtctgtct ctgcttagtg tggttcagtc ttaaacgctg
    18001 gattgctcct ttgaaggatt tgagagaaac catgttggaa attgcttctg gtgctcaaaa
    18061 tcttcgtgcc aaggaagttg gtgcctatga actgagagaa gtaactcgcc aatttaatgc
    18121 catgttggat cagattgatc agttgatggt agctattcgt agccaggaag aaacgacccg
    18181 tcagtaccaa cttcaagccc tttcgagcca gattaatcca catttcctct ataacacttt
    18241 ggacaccatc atctggatgg ctgaatttca tgatagtcag cgagtggtgc aggtgaccaa
    18301 gtccttggca acctatttcc gcttggcgct caatcaaggc aaggacttga tttgtctctc
    18361 tgacgaaatc aatcatgtcc gccagtatct ctttatccag aaacaacgct atggagataa
    18421 gctggaatac gaaattaatg aaaatgttgc ctttgataat ttagtcttac ccaagctggt
    18481 cctacaaccc cttgtagaaa atgctcttta ccatggcatt aaggaaaagg aaggtcaggg
    18541 ccatattaaa ctttctgtcc agaaacagga ttcgggattg gtcatccgta ttgaggatga
    18601 tggcgttggc ttccaagatg ctggtgatag tagtcaaagt caactcaaac gtgggggagt
    18661 tggtcttcaa aatgtcgatc aacggctcaa acttcatttt ggagccaatt accagatgaa
    18721 gattgattct agaccccaaa aagggacgaa agttgaaata tatataaata gaatagaaac
    18781 tagctaactc ccagtcaatt ctgggagttt tatgcgtaat tgggcaaact ttctaggttg
    18841 tctatcttgc taaaaacaag aaaaaacggt atgatagaag agtgacaaca gaggtaacag
    18901 agatgaacga gacacagatt caaagagaaa cacgtcaggt agtggaagat gttttagaaa
    18961 agaccaattt gaaacagggg gccctttttg ttttaggtct atcttctagt gaggtgctag
    19021 gtggtcagat tgggaaagaa tccagtcaag agattggtga gctgatagtg gagactatct
    19081 tgggtattct aggtagtaga ggtatccatt tagcagttca aggttgtgag catgtcaatc
    19141 gggctctcgt tgttgaacgt caggtggcag agcagtttga tctggaaatt gtcagtgtcc
    19201 atcctactct tcatgcagga ggttcggggc agttggcagc ctttaagttt atgcaggatc
    19261 cagttgaggt tgaatttatc aaggctcatg ctggattgga tatcggagac actgcaattg
    19321 gcatgcatgt caagcatgtt caggttccga ttcgccctat tttgagagag attggtcatg
    19381 cccatgtaac ggcactggct agtcgtccaa aattaatcgg aggtgcgcgt gcgcactatc
    19441 cgcaagatgc tattaggaag tcttgagaat gaataaagga gataaaagtg tttaaaaaag
    19501 accgtttttc aattcgtaag attaagggag ttgtaggctc tgtatttctt ggaagccttt
    19561 tgatggctcc ttctgtagtg gacgcagcca cctatcacta tgtaaataaa gagattattt
    19621 cacaagaagc taaagattta attcagacag gaaagcctga caggaatgaa gttgtatatg
    19681 gtttggtgta tcaaaaagat cagttgcctc aaacagggac agaagcatct gttttgacag
    19741 cttttggttt gctgactgtt gggagcttgc ttttaatcta caagagaaag aaaattgcta
    19801 gcgtctttct agttggagct atgggattgg tagttcttcc tagtgcaggg gctgtagacc
    19861 cagttgcgac cctagcgctg gctagtcgag agggtgttgt tgaaatggat ggctatcgct
    19921 atgttggtta tctatcaggt gacatcctca aaacgcttgg cttggacact gttttagaag
    19981 aaacctcagc aaaacctgga gaggtgactg tagtcgaagt tgagactcct caatcaacaa
    20041 caaatcagga gcaagctagg acagaaaacc aagtagtaga gacagaggaa gctccaaaag
    20101 aagaagcacc tagaacagaa gaaagtccaa aggaagaacc aaaatcggag gtaaaaccta
    20161 ctgacgacac ccttcctaaa gtagaagagg ggaaagaaga ttcagcagaa ccagctccag
    20221 ttgaagaagt aggtggagaa gttgagtcaa aaccagagga aaaagtagca gttaagccag
    20281 aaagtcaacc atcagacaaa ccagctgagg aatcaaaagt tgaacaagca ggtgaaccag
    20341 tcgcgccaag agaagacgaa caggcaccag tcgagccaga aaagcaacca gaagctcctg
    20401 aagaagagaa ggttgtagag gaaacaccga aacaagaaga gtcaactcca gataccaagg
    20461 ctgaagaaac tgtagaacca aaagaggaga ctgttaatca atctattgaa caaccaaaag
    20521 ttgaacaagc aggtgaacca gtcgcgccaa gagaagacga acaggcacca acggcaccag
    20581 ttgagccaag agaagacgaa caggcaccaa cggcaccagt tgagccagaa aagcaaccag
    20641 aagttcctga agaagagaag gctgtagagg aaacaccgaa accagaagat aaaataaagg
    20701 gtattggtac taaagaacca gttgataaaa gtgagttaaa taatcaaatt gataaagcta
    20761 gttcagtttc tcctactgat tattttacag caagttacaa tgctcttgga cctgttttag
    20821 aaactgcaaa aggtgtctat gcttcagagc ctgtaaaaca gcctgaggta aatagcgaga
    20881 caaataaact taaaacggct attgacgctc taaacgttga taaaactgaa ttaaacaata
    20941 cgattgcaga tttaaaaaca aagacgaaag aacatcatag tgatacaagt tggcaaaacc
    21001 ttcaaactga agtcacagag gctgaaaaag ttgcagctaa tacggatgct aaacaaagtg
    21061 aagtagattc tgagactgca agtcttaaaa cagcaatttc tggattaaat acagataaag
    21121 ctgaactcga aaatcaactt aagattgcac aggaaaaaac tgaaactgat ttcagcatgg
    21181 gatcttggac ggttttgagt acagctcaaa ataaagctca ggaagtaaaa gataacggca
    21241 cagcgaccca agaccaaatc aacgaagcag agcaaaattt gaaaacagcc ctcgctggtt
    21301 tgactgttga taagtctcaa cttgaaagca aacttacaga tacggcatct aaggtttcaa
    21361 cagactacag caagactagt tgggagaaga tgactgtggc taaagaaacc gctactgttg
    21421 ttaaggagaa tcaagaagcc aagcaatcac aaatagatga atcagcaaaa gctttggaaa
    21481 cagcccttgc tgacttatct gttgacaaaa cagctttggg aagtgctatc gatacagcta
    21541 ccaagaaaaa taaagagaac tatacaaatc aaacatgggc agagttagaa acagttctta
    21601 cagcagcaaa atctgtaaac actaacgagt ctaaacaaag tgaagttaac gaagctgttg
    21661 aaaaattaac tgcaactatt gaaaaattgg ttgaattatc tgaaaagcca atattaacat
    21721 tgactagtac cgataagaaa atattggaac gtgaagctgt tgctaagtat actttaactt
    21781 caacaaaagc taaaatcaaa tcaatcacag ctgaattgaa aaaaggtgat actgttattc
    21841 atacggtgat attagcagga gataatctga aagaagctgc tcttagtgca gaatttaaca
    21901 acttagaata ttataaggaa tatactttat ctacgaaaat agtttacgat agaggtaacg
    21961 gtgacgtaac agaaactcta gaaaatcaaa atattcaatt agatcttaaa aaaattgaga
    22021 ttaaaaacat caatcaaaca agtttaataa gtgttgatgc tgatggtaat gaatcagata
    22081 aaagtttatt atcagaaaaa ccaacagaca tttcatcata ttacttaaaa gttacaactc
    22141 acgacaacaa agtgacaaga cttgctatcg atacgattga agaagtagaa gaaggtggta
    22201 aaactttatt taaagttact gctactgctc ctgatttaat ccaacgcaac agtgaaaata
    22261 agctaagcga aacttacact cactacttcg aaaaacaaat accaaaagta gataatgtat
    22321 attataactt taacgagtta attacagata tgcaaaaaaa tcctaatggc atattcaaac
    22381 ttggtgcaga tcttaatgca gcaaatgtaa aacctaaagg gaaatcttat gtaactacta
    22441 agtttagagg agaattatta agtgctgatg gcggtcatta tacaattcat aacatagaac
    22501 gtcctctatt ggctgatgct gaaggagcta acatccatga cattaatctt gcgaatgtaa
    22561 atattaatat agcttgggca gataaagttg ctcctcttgg taatatgttt aaaaaatcaa
    22621 gtattagaaa tataaaagtt atagggacta tcaccggtaa taatgatgtt accggtatgg
    22681 ttaacaaact tgatgaatca aatatgagaa acgttgcatt tataggtaaa attaatagcc
    22741 ttggtaacaa aggttggtgg tctggaggac ttgtgagtga aagttggata agtaatgttg
    22801 ataacgctta tctagatgcc gatattgtag gtaataattc caagttcggt ggattaatcg
    22861 ccaagttaga tcatggcgta gaccaattca cagttggtaa aaaaggatac ttgaaaaact
    22921 catttgttaa aggtacaatg aaacttactc atcatggtga gagtggtgga ttaattcata
    22981 acaatttcaa ctggggagtt gtagaagata ctatcagtat gatgaaagta acaaacgggg
    23041 aaatcatgta tggttcacgt gatttaaacg gagatgatca atacttcggt ttagataatt
    23101 tcaaacgaac tttctatgtc aatggtgtct ctgagggatt aatgtcttat aaaaaatcta
    23161 ataatatcca gggtatctct aaggagcagg cactagaaaa atttgctaaa atggggatta
    23221 ccgctcatga atttacaatt accgagccaa ttgtaaataa acttaacggt ataaaaccaa
    23281 aagctgatac ttataaagat actcaagatt acaaagcaga ccgcgaactt gcttatcgaa
    23341 acattgaaaa atttcaacca ttctacaata aagagtggat tgtagagcaa ggtaatagat
    23401 tggctacagg ttcaagccta atgactaaag aagtgttatc tgtaacaggt atgaaagatg
    23461 gtcaatttgt gactgactta tcagaaattg ataaaattat gattcactat gccgatggaa
    23521 ccaaagaaga gttagctgta actgctaaaa cggactctaa agtagctcaa gtaaaagggt
    23581 atgatgtggc aggtcaaaac attatctata ctccaaatat ggttatgaag aatcgtaacc
    23641 agttagcaag cggtattaaa gagaagttag ctagtgttac cttactttca gatgaagttc
    23701 gtagcttgat ggatcagcgt gataaacctt ggaagaacac accagataaa aaaactgagt
    23761 atattaaagg cttgtatttc gaagaaagct ttgaagaagt aaaaggaaac ttagaaaaat
    23821 ttgttagtaa gattttagaa aatgaagatc atcagttaaa tggtggggaa gtagttgagc
    23881 gtgctttact taagaaagtt gaagataaca aagctaaaat tatgatggga ctgacttatc
    23941 ttaatcgtta ctatgatatc aaatatggtg atttgagtat caaagatatt atgatgttta
    24001 aacctgactt ttatggtaag acaccaagtg tattagatcg tttgattcaa attggttcaa
    24061 gggagcattt cttgaaaggt gaccgtacac aagatgctta caaagaagtg attgctggtg
    24121 caactggtaa aggtgattta cgtagcttct tagactataa catgcgttta tttacagaag
    24181 ataaagattt gaatgactgg tttattcatt ctgcgaaaaa cgtttatgta gtagaacctg
    24241 aaacaagtac agaagccttt aaagataagc gtcaccgtgt atttgatggt ttaaacaatg
    24301 atattcacgg tcgtatgatt ttaccattgc tcaacttaaa gaaagctcat atctttatga
    24361 tttcaaccta taacacttta gcttatagtt catttgagag atatggtaaa aatacagagg
    24421 aagcgagaga gtctttaaaa cctaagatta tttcagtagc aaaagcacaa caaagatacc
    24481 tagacttttg gtctcgttta gctcttccaa gtgtacgtga taagctcctc aagagtcaaa
    24541 atatggtacc gactcctgtg tgggacagtc agtggtatga cggtattcct gacgcaaatc
    24601 gtcaaggtta tggacgaggt ggtgccgtgg tatctcctat tcgtgagcta ttcggcccga
    24661 ctgatcgttg gcatcaagtt aacggagcga tgggagcgat ggcaaaaatt tatgaacgtc
    24721 catggaaaga tgaacaagtt ttcttcatgg taacgaatat gttagaggac ttcggtattt
    24781 cagcatttac gcatgaaacg acacacgtta acgaccgtat ggcttactta ggtggatgga
    24841 gacatcgtcc gggaactaat gttgaagcgt ttgcgcaagg aatgttacaa tcgccttctg
    24901 aaattgggca ccaaggagaa tatggagcgt taggattgaa tatggcttac gttcgtccaa
    24961 acaatggaaa tcaatggtat aatcctgacc ctgaaaaatt acaaactcgt gaacaaattg
    25021 atcgctacat gaagaactac aatgatgctc ttatgatgtt agaccatctt gaggctgatg
    25081 cggttatccc taaagtacaa gacaatatta gccgttggtt caagaaaatg gatagacaat
    25141 atcgtagtgg aaattctggt aatcagttcg atagagttcg tgaattgaat gaagatgaga
    25201 agaataactt agttcttaat actattgatg atttagttac taataatttg ataacaaaac
    25261 atggtgctcc tagcgataga atttacaatc ctgaagactt tgactctgct tatgtcaata
    25321 taaatatgat gactggtatt tatggtggaa atacaagtac aggtgcaccg ggtgcagcat
    25381 catttaaaca taatacattc cgtatgtggg gttacttcgg atacgaaaat ggatttgttg
    25441 gatatgcttc tagtaagtat caagatgcag ctaataaaga aaataataag atgttaggtg
    25501 atgacttcat tatcaagaaa gtatctaata atagatttag tactcttgaa gaatggaaaa
    25561 aagcttatta tcaagaagtg aaggcaaaag ctgacaaagg atttactgca attgaaattg
    25621 atggtaaaca aattactaat tatgctgagc taaaagtctt atttgataag gcggttgagg
    25681 ctgatctagc aggtggagga acagctaaaa ctgtagagct taaatcgaaa gtctttaaag
    25741 ctctccttaa aaacacagac ggtttctctg gtgatctatt tactacacca caagcatagg
    25801 gatgaataaa atcgtaaaaa ggtagattaa gtttttcttg atctgccttt ttatgctata
    25861 cttaaggtat gcatagaaaa acagtgattg attttagggt tttgggggag agatacacct
    25921 ttacccagcc tattaaagag ttgaaaacga gaaatgtaga agaagtggca gatttgctgg
    25981 cacaagtgga aagctaccaa gagcaagatt attatgtggt ggggtatgtc agctacgagg
    26041 ctgcacctgc ttttgaggaa aaattagcag ttcacaagga tcccctactg ggagagtacc
    26101 tgctttattt tactgttcac gatagggtag agacctcccc tattcctctg acttatgagg
    26161 atattgattt gccctcaaat tggcaggaag taacgtctgc agcagactat gaaaaggcca
    26221 ttgcccaaat tcaccatcat ttacggcagg gagataccta tcaggtcaac tacaccgtcc
    26281 aacttaagca aaagttaaat gccaatcctt ttgccatcta caatcgtatg gtggtagagc
    26341 aggaggcggg ctacaatgcc tatgtggaac atgacgagat ggcagtgatt tccatgagcc
    26401 cagagctctt ttttgagcaa aatgatcgcg agttgacaac acgaccaatg aaggggacga
    26461 ctcagcgtgg ggtaactgac caagaagatc ttgaacaggc cagttggttg gaacaggatc
    26521 ccaaaaatcg ctctgaaaat atgatgattg tggacctctt gcgcaatgat atgaaccgta
    26581 tttctgaagt tgggagcgag cacgtggagc gtctgtgtca ggtagagcag tattcaactg
    26641 tttggcagat gacttcgacc atcaagagtc agttgcgaga ggatgtggac cttgttgaaa
    26701 tcttccgctc actctttcct tgtggttcca taacgggtgc accgaaaatt gcgacaatgg
    26761 agattatcaa ggacttggag cctcaaccgc gtggagtcta ctgtggaacg attggtctct
    26821 tgcttccaaa tggacgacgg atttttaatg tggccattcg taccattcaa cttcacaaag
    26881 gtcaagccat ttatggagtt ggcggaggga ttacttggga tagcacatgg gaatctgaat
    26941 accgagaggt tcatcaaaag gcagcagttc tttatcgtaa acaagctcgc ttccaactga
    27001 ttacgacagg ggaaatcagc cagaagaacc tgctgtttga agatcaacat ctggaaagac
    27061 tgagaaaagc tagtcgttat tttgcttttc cttttgatgc agaagacttg ggacacaaga
    27121 ttgaggaaga gtgtcaggat tgtgaagcta atcgagatta ccgcttgcga atcagcctta
    27181 acaaatctgg agagatagaa gtcaatcgtc aagtattaac ccctctcagt acaagctttt
    27241 gtcaggccca agtctgtctt caggaagctg ctttgaatca atcctttacc tactttaaaa
    27301 ccactcaccg accgcatttg agcctaggag aacaagagaa gatttaccac aataagtcag
    27361 gagaactgct tgaaacctct ataggaaatt tggttctgaa aatcgctgga aaactctaca
    27421 caccgcctat ccgacttgga atcttgccag gaatttaccg tcagcatttg ctagaaacag
    27481 gacaggtaga agagaaagtc ttgaccttgg cagatttaac ccaagcagaa gctatttacg
    27541 gctgtaatgc agtgagaggc ttgtatgaat taagcctaga ggaaaattag atgaaactgg
    27601 tatttttaca cggtttaggg gagtcagcag agtcttggaa agaagttcgg aatcaattga
    27661 aggataagcc cacagagact cttgaattgc tgaaataagt ttgataaaat aatattgaaa
    27721 tcgatatcat aattatggga gaaagaaatg aataaacgtc tatttttaaa aatgagtctg
    27781 gtgacgttgc caattttagc cttgttttca caaccagttt tggcggaaga aaacatccat
    27841 ttttcgagct gtaaggaagc ttgggcgaat ggctattcgg atattcacga gggagaacct
    27901 ggttattctg ccaagttaga ccgtgatcat gatggtgtgg cttgcgaatt gaaaaatgct
    27961 cctaagggtg cttttaaagc aaaacagtca acggctattc aaatcaacac aagttcagca
    28021 acaacaagtg gttgggttaa gcaggacggc gcttggtact actttgatgg aaatggaaat
    28081 ctagtgaaaa atgcatggca gggaagctat tacctgaaag ctgatggtaa aatggcccag
    28141 agtgaatgga tttatgactc ttcttatcaa gcttggtatt atttgaaatc agatggttct
    28201 tatgcaaaaa atgcatggca aggagcttat taccttaaat caaacggtaa aatggcacaa
    28261 ggtgagtggg tttatgatgc
//
LOCUS       DAVDWM010000024        27149 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVDWM010000024 DAVDWM010000000
VERSION     DAVDWM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.024"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VUW49_001230"
     CDS             136..2316
                     /locus_tag="VUW49_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET7981150.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVTAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VUW49_001231"
     CDS             2368..3183
                     /locus_tag="VUW49_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7981151.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALSAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            3323..4666
                     /locus_tag="VUW49_001232"
     CDS             3323..4666
                     /locus_tag="VUW49_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7981152.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQFMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     TPRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            4881..6071
                     /locus_tag="VUW49_001233"
     CDS             4881..6071
                     /locus_tag="VUW49_001233"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7981153.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6624..7559
                     /locus_tag="VUW49_001234"
     CDS             6624..7559
                     /locus_tag="VUW49_001234"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET7981154.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7593..8630
                     /locus_tag="VUW49_001235"
     CDS             7593..8630
                     /locus_tag="VUW49_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7981155.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8803..9408
                     /locus_tag="VUW49_001236"
     CDS             8803..9408
                     /locus_tag="VUW49_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7981156.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9566..10096
                     /locus_tag="VUW49_001237"
     CDS             9566..10096
                     /locus_tag="VUW49_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET7981157.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            10117..11019
                     /locus_tag="VUW49_001238"
     CDS             10117..11019
                     /locus_tag="VUW49_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET7981158.1"
                     /translation="MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            11019..11501
                     /locus_tag="VUW49_001239"
     CDS             11019..11501
                     /locus_tag="VUW49_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7981159.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            11503..13044
                     /locus_tag="VUW49_001240"
     CDS             11503..13044
                     /locus_tag="VUW49_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7981160.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEMERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(13100..13903)
                     /locus_tag="VUW49_001241"
     CDS             complement(13100..13903)
                     /locus_tag="VUW49_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7981161.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(13987..14199)
                     /locus_tag="VUW49_001242"
     CDS             complement(13987..14199)
                     /locus_tag="VUW49_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981162.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(14678..14887)
                     /locus_tag="VUW49_001243"
     CDS             complement(14678..14887)
                     /locus_tag="VUW49_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981163.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            15057..15329
                     /gene="rpsP"
                     /locus_tag="VUW49_001244"
     CDS             15057..15329
                     /gene="rpsP"
                     /locus_tag="VUW49_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7981164.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            15349..15588
                     /locus_tag="VUW49_001245"
     CDS             15349..15588
                     /locus_tag="VUW49_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET7981165.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            16165..16953
                     /locus_tag="VUW49_001246"
     CDS             16165..16953
                     /locus_tag="VUW49_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7981166.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     RATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            16984..17502
                     /gene="rimM"
                     /locus_tag="VUW49_001247"
     CDS             16984..17502
                     /gene="rimM"
                     /locus_tag="VUW49_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7981167.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            17492..18211
                     /gene="trmD"
                     /locus_tag="VUW49_001248"
     CDS             17492..18211
                     /gene="trmD"
                     /locus_tag="VUW49_001248"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7981168.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            18223..18561
                     /locus_tag="VUW49_001249"
     CDS             18223..18561
                     /locus_tag="VUW49_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981169.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(18592..18912)
                     /locus_tag="VUW49_001250"
     CDS             complement(18592..18912)
                     /locus_tag="VUW49_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981170.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            19010..19225
                     /locus_tag="VUW49_001251"
     CDS             19010..19225
                     /locus_tag="VUW49_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET7981171.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(19294..19374)
                     /locus_tag="VUW49_001252"
                     /pseudo
     CDS             complement(19294..19374)
                     /locus_tag="VUW49_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(19430..19966)
                     /locus_tag="VUW49_001253"
     CDS             complement(19430..19966)
                     /locus_tag="VUW49_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET7981172.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQTYFTN"
     gene            complement(20163..21509)
                     /gene="gor"
                     /locus_tag="VUW49_001254"
     CDS             complement(20163..21509)
                     /gene="gor"
                     /locus_tag="VUW49_001254"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET7981173.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSINGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            21795..22994
                     /locus_tag="VUW49_001255"
     CDS             21795..22994
                     /locus_tag="VUW49_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7981174.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PIHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KTDEATN"
     gene            22978..23679
                     /locus_tag="VUW49_001256"
     CDS             22978..23679
                     /locus_tag="VUW49_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981175.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            23681..24940
                     /locus_tag="VUW49_001257"
     CDS             23681..24940
                     /locus_tag="VUW49_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981176.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            25064..27061
                     /gene="metG"
                     /locus_tag="VUW49_001258"
     CDS             25064..27061
                     /gene="metG"
                     /locus_tag="VUW49_001258"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7981177.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQVYVDRMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEVSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
BASE COUNT         8302 a         4979 c         5906 g         7962 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaagtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatctttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 gctagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agtgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgacgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtcatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaagat
     1861 ctcactgagg aagtttactc agtagcagac ggtcaagttg ttgctttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgta tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttac agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gtcagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccatgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactgtagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatatt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 ggtagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagaca agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaact gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacca aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccagttg actcagttta tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggcgatatt acccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttggcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcatcgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagacgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa accaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtattgagct ccaacttttt gctttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtactt tgtaactcta taacaatatt
     4861 ttttaagggg ggacattttt atgtcagagc gtaaattatt cacgtctgaa tctgtatctg
     4921 aggggcatcc ggataagatt gcagaccaaa tttcagatgc gattttggat gctattttag
     4981 caaaggatcc agaggcgcac gttgctgctg aaacagctgt atatactggt tctgtccacg
     5041 tttttggtga aatttctaca aatgcctatg tggatattaa ccgtgtggtt cgtgatacca
     5101 ttgcagagat tggttatacc aatacagaat atggattttc tgctgagaca gtgggagtac
     5161 acccatcttt ggtggaacaa tctcctgaca tcgctcaagg tgttaacgaa gccttggagg
     5221 ttcgtggaaa tgctgatcaa gatccactgg acttgattgg agcaggtgac caagggctca
     5281 tgtttggatt tgcagtagat gaaacagaag agctcatgcc attgccaatt gcactcagtc
     5341 ataaattggt tcgtcgtctg gcagaacttc gtaagtctgg agaaattagc tatctccgtc
     5401 cagatgcaaa atcacaagtt acagttgagt acgatgaaaa tgaccgtccg gtacgtgtag
     5461 atacagtcgt tatttctact cagcatgatc cagaggccac taatgaacaa atccatcaag
     5521 atgtgattga caaggtcatc aaagaagtta ttccatcttc ttatcttgat gataagacaa
     5581 aattctttat caatccgaca ggtcgttttg taatcggtgg tcctcaaggg gactcaggtt
     5641 tgactggtcg taagattatt gtagatactt atggtggcta ctctcgtcat ggtggtggtg
     5701 ccttctctgg taaagatgcg actaaggtgg atcgttcagc ctcttatgcg gctcgctata
     5761 ttgccaagaa tatcgttgca gcaggccttg ctaagaaggc agaagtgcag ttggcctatg
     5821 ctatcggtgt tgcgcaacct gtttctgttc gtatcgatac tttcggtaca ggaacagtag
     5881 ctgaaagtca acttgaaaaa gcggctcgtc aaatctttga ccttcgccct gcagggatta
     5941 tccaaatgct ggacctcaag cgtccaattt accgtcaaac atcggcttac ggtcacatgg
     6001 gacgtacaga tattgatctt ccatgggaac gtttggataa ggtagatgct ttgaaagaag
     6061 cagtaaaata agattttaag aggggaacgt cctctctttt ttatagtttt taactatact
     6121 gggatactgt tctgaaaatc cattttgcga aagtagagat ttacatgtat agtagattga
     6181 aactagaata gtacacctca acttctaaaa cattgttaaa aatcgatttg actgtcctga
     6241 tcgatttctc ctgttcttgt ttcattttac tatatttctt taaaaatgat aaaggttgag
     6301 atttctcctc gtaatagata atcttgggga tatttcaatc caaagtttta ttcgttatca
     6361 cttgactatt gcaaggtttt ctagagcaac agagtcatgg aatggactca tggttgagat
     6421 ttctccttgt tgcttggact tcattcaaaa gtctgttacc caagccttgt tcaaacttct
     6481 aatacactag ctgtttccat agcatgactt ctgtactaga ctttcttttc cgaataaata
     6541 gatagaacca cagaatctag taaacctaga attaaaatta tggtataata ttagcaataa
     6601 aagaaatctg gaggattaga atcatggtat caacgaaaac acaaattgct ggttttgagt
     6661 ttgacaattg cttgatgaat gcagcaggtg tggcttgtat gacgatagag gagttagaag
     6721 aggtcaaaaa ctcagcggca ggaacctttg ttactaagac agcgaccttg gacttccgtc
     6781 aggggaatcc tgagccacgc taccaagatg ttccacttgg ttccatcaac tctatgggct
     6841 tgccaaataa tggcttagac tattatttgg attatctttt ggatttgcag gaaaaagagt
     6901 cgaaccgaac tttcttctta tctctggtcg gcatgtctcc agaggaaacc catactattt
     6961 tgaaaaaagt ccaagagagt gattttcgtg gtctgactga gctaaatctt tcctgtccaa
     7021 atgttccagg taaacctcag attgcctatg attttgagac aacagaccgg attttggcag
     7081 aagtgtttgc ttacttcacc aaacctcttg gaattaaatt gccaccttat tttgatattg
     7141 ttcactttga ccaagcggca gctattttca acaaatatcc gctcaagttt gtcaactgcg
     7201 ttaactctat cggaaacggc ctctatatag aagacgaatc tgtcgttatt cggcctaaga
     7261 atggttttgg tggaattggt ggagaataca tcaaaccgac tgctctagcc aatgttcacg
     7321 ccttttatca acgtttaaat cctcaaatcc aaattatcgg aacaggtggc gttctgactg
     7381 gtcgagatgc ctttgaacac atcctctgtg gagcaagtat ggtgcaggtg ggaacgaccc
     7441 ttcacaaaga aggcgtcagt gcttttgacc gcattaccaa tgaactgaaa gcaatcatgg
     7501 tggaaaaagg ctacgagagc ttagaagatt tccgtgggaa attgcgctat attgactaaa
     7561 ttaaatcgaa aaatcagaag aaagaaggga cgatgctagc cattgaagaa agtcagaagt
     7621 tgactttatc aaatttaccg agcctgagcc tatttacagg gacagatcag ggtcagtttg
     7681 aagtgatgaa gagtcaaatg ttgaaacaga ttgggtatga ttctgctgac ctcaactttg
     7741 cctactttga tatgaaagaa gtagtttaca aggatgtgga actggagttg gtcagccttc
     7801 ctttctttgc ggatgagaaa atcgtgatat tagattattt tatggatatc acgactgcta
     7861 agaaacgctt tttgacagat gatgagctta agtcatttga ggaatacctt gacaatcctt
     7921 ctccaacaac caagttgata atctttgcag aaggaaagct ggatagcaaa agacggttag
     7981 tcaaattact taagcgtgat gccaaggtct tcgatgcagt agaagtaaaa gaacaagaat
     8041 tgcgccagta tttccaaaag tggagtcaga aacaaggtct gcagtttacc aatcattctt
     8101 ttgaaaatct cctcatcaag tcggggtttc aatttagcga aatccagaaa aatcttctct
     8161 ttttacagtc ctataaggcg gattctgtta tcgaggaaga ggatattgtt aacgcaattc
     8221 ccaagacctt gcaggacaat atttttgatt taactcagtt tattctgact aaaaagatgg
     8281 atcaggcgcg cgatttggtg agagacttga ccttgcaagg ggaagatgaa atcaaactga
     8341 ttgcagtcat gctgggacaa tttcggactt ttactcaggt gaagattttg gcggagtctg
     8401 gccaaacaga atcgcagatt gcaagtagtt taggtagtta tctgggacgt aacccaaatc
     8461 cttatcaaat caagtttgca ttaagagatt cgagaggact ttctttgagc tttctgaagc
     8521 aagctatttc ctatttgatt gagacagact atcagattaa gacaggtctt tatgaaaaag
     8581 gtttcctttt tgaaaaggca ctcttacaga ttgctagtca ggtcaattga catttgttga
     8641 aactacaacc cgcgtcagga ttatcttgtc gtgggtttct tgctgatttg cttattttat
     8701 tagaaagtat gtcagtgatt tccttatcta taagcaaaaa acttgaaaaa agtgaatgtt
     8761 tgcgttatca ttttcatata gaaagaaaaa gaggtattac agatggctat tatcttacca
     8821 gaacttccat atgcatacga cgctttggaa ccatacatcg atgcggaaac aatgcatttg
     8881 caccatgaca aacaccatca aacttatgtc aacaatgcca atgcagcttt agaaaaacac
     8941 cctgaaatcg gtgaagacct tgaagccttg cttgctgatg tagaatctat cccagctgat
     9001 atccgtcaag cacttatcaa caatggtggc ggacacttga accacgctct tttctgggaa
     9061 ttgatgactc ccgagaaaac agctccttca gcagaactgg cagcagcaat cgatgcaaca
     9121 tttggttcat ttgaagaatt ccaagcagcc ttcactgcag cagcaacaac tcgttttggt
     9181 tcaggttggg catggttggt tgtcaacaaa gaagggaaac ttgaagtgac ttcaacagca
     9241 aaccaagaca caccaatctc agaaggtaaa aaaccaatct tgggcttgga cgtttgggaa
     9301 catgcttact acgtgaaata ccgcaacgtg cgtcctgact acatcaaagc tttcttttca
     9361 gtaatcaact ggaataaagt agatgaattg tacgcagctg ctaaataatg atagttggag
     9421 ggaagaattg ttcttctctt ttttaggtta taagattctg gtctgacaaa atcgttagac
     9481 ttttttcatt ttttatgaga aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt
     9541 aagtaatcgt ggaaaaagga tatctatgcg aaaagaaatt gcacctgaat tatacaacta
     9601 taacaagttt cctggtccgg agttccattt acacggggac aaggtcgaaa cggaagggat
     9661 agctttttcc ttggttgaaa atatcaagga tgcctttgat gtgacgactt ttaatcagcg
     9721 tttttcagaa gtcttaacca agtttgatta tatcgtgggg gactggagca acgaacagct
     9781 tcgcctacga ggtttttaca aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg
     9841 tttacaagac taccttttag agtattgtag ttatggttgt gcctattttg tcttagaaaa
     9901 tgaagcccct aagcgagcat catttgacaa gaaaatgcgt aagacggaag aagaaacacc
     9961 ttctagaaaa ggaaagaaac cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa
    10021 tagacgtcgt cagaaagacc agcattctca gaaagaggac aagggacaac gtcattttgt
    10081 cattcgtcag aagtgagtag agaataagga gaagaaatga aaccttcaat tcatagtttg
    10141 acacatcaaa ccatgcagga atgggtattg gagcagggag aaaagaaatt ccgtgcagat
    10201 caaatctggg aatggctcta ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt
    10261 tccaaggatt tgattgctaa gctcaatgac cagtttgtgg tcaatccctt gaaacaacgt
    10321 attgtgcaag agtctgctga tggtacagtc aaatatctct ttgaattgcc tgacggtatg
    10381 ttaattgaga ctgtactcat gcgtcaacac tatggtttat ctgtctgtgt gaccactcag
    10441 gtcggctgta atatcggttg taccttctgt gcctctggtt tgattaagaa gcagcgtgac
    10501 ctcaataacg gtgaaatcgt agcgcaaatc atgctggttc agaaatactt tgctgatgaa
    10561 ggggttcagg tcaatcttgc cgtgtctctt cacgcaccca acaatgaact tcgttcaagt
    10621 atcatgaaga ttaaccgtgc ctttccgatt gaaaaactct ttgctgctat tgaatactac
    10681 attgagacaa caaaccgtcg tgtaaccttt gaatatatca tgcttaatga agtcaacgac
    10741 ggtgtagaac aagccttgga attggctgaa ttgctcaaga acatcaagaa attgtcttat
    10801 gtaaacttga ttccttataa cccagttagt gagcatgacc aatatagccg tagtcccaaa
    10861 gagcgcgtgt tggctttcta tgatacgctt aagaaaaaag gggtcaactg tgtggttcgt
    10921 caagagcatg gtacagatat tgacgcagct tgtggacaat tgcgctctaa cacaatgaaa
    10981 cgtgaccgcc agaaggcagt cgcagcggta aatccttaat gatgaagaag acttataatc
    11041 atattttggt ctggggagtc attttctata gcatttgcat tgtctgtttt tgctttactc
    11101 ctcaagaaca atctaccgtg ggagtgggaa ctccaggtat tcagcatctt ggacgcctgg
    11161 tttttctttt gactcctttc aattctctct ggaaactggg cgaagtgagt gacattggac
    11221 aattatgttg gattttttta caaaatgtcc tcaatgtctt cttgtttttt cctctgattt
    11281 tccaactcct ttatctattt ccaaatttgc ggaaaacaaa aaaggtcctt ctttttagtt
    11341 ttcttgtgag tcttggaatc gagtgtacgc aattaatctt ggactttttc tttgatttca
    11401 atcgcgtctt tgagattgat gatttgtgga ccaatacttt gggtggctat ctggcttggc
    11461 tcctttataa acgattacat aaaaacaaga taaggaatta aaatgagtat tttagaagtt
    11521 aaaaatctga gtcacggttt tggtgaccgt gcaatttttg aagatgtgtc cttccgtctc
    11581 ctcaagggag aacatatcgg cctggtcggt gccaatggtg aaggaaaatc aacctttatg
    11641 agtatcgtga ctggtaaaat gctgccagat gaaggaaagg ttgagtggtc caaatatgtg
    11701 acggctggtt acttggatca gcactctgtc cttgctgaaa gacagtcggt gcgtgatgtt
    11761 ctccgtacgg cttttgatga gcttttcaaa gctgaagctc gtatcaatga cctctatatg
    11821 aaaatggctg aagacggcgc ggatgttgat gctctcatgg aagaagtagg agaacttcaa
    11881 gaccgtctgg agagtcgtga tttctatacc ttggatgcta agattgacga agtagcgcgt
    11941 gctcttggtg ttatggactt tggcatggat acggatgtaa cttctttgtc aggtgggcaa
    12001 agaaccaagg tgcttttggc aaaacttctc cttgaaaagc ctgatatttt gctgttggac
    12061 gagccgacca actacttgga tgctgagcat attgattggc tcaagcgcta tctccaaaac
    12121 tatgagaatg cctttgttct catttcgcac gatattccat tcctcaatga cgttattaat
    12181 attgtctatc atgtggaaaa tcaacagttg acgcgttact ctggtgacta ctaccagttc
    12241 caagaagttt atgctatgaa gaaatctcag ctagaggcag cctacgaacg ccagcagaaa
    12301 gagattgcag acctcaagga ctttgtggct cgtaataaag cccgtgttgc aactcgtaat
    12361 atggctatgt ctcgtcaaaa gaaattggat aagatggata ttatcgaact ccaaagtgag
    12421 aaaccaaaac catcctttga tttcaaacca gctcgtacac cagggcgctt tatcttccaa
    12481 gccaagaact tgcaaattgg ttacgaccgt cctcttacta agcctttaaa tcttaccttc
    12541 gaacgcaatc aaaaggttgc gattattggt gctaatggta ttggaaaaac aactctcttg
    12601 aagagtctct taggcattat ctcgccaatc gctggggaaa tggagcgtgg agattattta
    12661 gaacttggtt attttgagca ggaagtagaa ggcggtaatc gccaaactcc tcttgaagct
    12721 gtctggaatg cctttcctgc ccttaatcaa gcagaagtcc gtgcagccct tgcccgttgt
    12781 ggtttgacaa ccaaacatat tgaaagccag attcaagtat tatcaggggg agagcaagcc
    12841 aaggttcgtt tctgtctctt gatgaatcgt gaaaacaacg ttttagtgct ggacgagccg
    12901 accaaccatt tggatgtgga tgcaaaggat gagctcaaac gcgctctcaa agaatatagg
    12961 ggatctatcc ttatggtctg ccacgagcca gacttttatg aaggctggat agaccaaata
    13021 tgggatttta ataatttaac ttaagaatga gagtaaaaga aatacagtac cgaatgtgga
    13081 tactgtattt ttttggtttt taagatttaa aatcgtagtc tttcaccact tcgatgctgt
    13141 cgattgtgat agcagtagtt ggcttgtctt tttcatcttt ttcggcctta gcaatcttat
    13201 ccacaacatc cataccgtca atcacttgac caaagactgg gtgtttgcca tctagactag
    13261 ggtttccacc ttctttgtag gcttcgataa ttttctgtgg atacttgctt gtagggagtt
    13321 tagaagaggt atctgtagag ttttggttga tgaagaactg gctaccattg gtgtttggtt
    13381 gaccagtatt agccatagca agagcaccac ggatgttata caaataagga gtaatctcgt
    13441 tcttgaaacc agttcctttg tctttagtct tatccttgtc atgccagatg gactgaccac
    13501 ctgtaccgtc cccttttgga tctccagttt ggaccataaa gccatcgatg acacggtgga
    13561 aggtaatacc gttatagtag ccttctttgg catgagtgag gaaattttca accgctagag
    13621 gagcgagttt agggaagagt ttaatgcgaa tatcaccttg gcttgtgtgg agaatcactt
    13681 cggcttcatc ttcagcaact tccttagata gttgtgggaa attggcgttt tcgtttgtta
    13741 atgcatcgtt taactccttg gcagattggg cagctacttt ggaactttct tcagcagcaa
    13801 gactggaatc aacataatca tcaccacgca gactgcgttg gacgctgcta cacccagcta
    13861 gggctacagt agacagtaaa agaagggttg ctagtttttt cattctgatc ctctcaaaaa
    13921 ttcatttata accattgtac cctaaaagga agtggatttc aaatattagt agtttacaga
    13981 actggcttag ataatagttg ggatttcatc atcatcttga aaatcttcaa cttcttcccc
    14041 agtaaatggt ttctgaaaag aacttagtat aaaacgagca attggttcaa caattaggaa
    14101 atttagagga agagctgcaa taaagtttac gaaccaggcg cttagatagg tattgggtgt
    14161 gatagcaatg ccattgtaaa aaagtccata gaaggacatg aaagtgacca gctaattctt
    14221 cttctgaaaa atgctcatga agggcttgat ccaggtcgca agatttcatt ttagaaagtc
    14281 cagtaactcc cttcttcttc aggatattct ttttcatggt attatttaga tggtcaagtg
    14341 agtcaaaaga agtttcgaca agagcatacc ctttttctac cagagttttt agatgtttag
    14401 gggcatcaat accataagtg tactcaaagt attttggaaa ccagatctta agaaagctcg
    14461 taaagctagc cagttctcta aacgctaaga actagctaca acacagcatt tacaacactt
    14521 catggttcac accatggggt gttttttaat gattttaagc aaaattcaca cttaattgaa
    14581 agttatttaa attagatgaa aatttatttt ttgaaaaagt gggaaaacat tgattctaag
    14641 cgaattttga atcatattcg aatgggagtt tttaaaatca tgtttcagtg gtaaaagtac
    14701 caaactggat tctccatagt aaaatgatat ctcctgctag tgaatcatct actaataagt
    14761 ccatatttcg cttgggaacg ggcttgggac ttagaagcca agtatccaac tctcgttcag
    14821 gcgatatgaa gggtttgact tggtgttctt tataaaattt ttctaaaata gtattcacgg
    14881 atagcatctt tcaaacattt atttcctatt ctaacaaatt tctaaatact ttaagaaatt
    14941 tatagtctgt caagtttttt tcttgacaca tgccagaaat ctgctataat agaacatgtg
    15001 ctaaatagct cagctatttc accgaaataa aaaattaaag aaatgagact ttaaaaatgg
    15061 cagttaaaat ccgtttgact cgtatgggtt ctaagaaaaa acctttctac cgtatcaacg
    15121 tagcagattc acgttcacca cgtgacggac gtttcatcga aacagttgga acttacaacc
    15181 cacttgttgc tgaaaaccaa gtaactttga aagaagaccg cgttcttgca tggttggcta
    15241 atggagctca accttcagac acagtacgta acatcctttc aaaagaaggc gtattgaaaa
    15301 aattccacga ttctaaattt tcaaaataag tttaaagtag gttgacagat ggatacgatt
    15361 gaaaatctca ttattgcgat tgtgaaaccc ttaatttcac aaccagatgc cttaactatc
    15421 aagattgagg atacaccaga atttttggaa tatcatttga atcttgatca aagcgatgtg
    15481 ggtcgtgtaa tcggtcgtaa gggtcgcact atttctgcga taagaacgat tgtctactct
    15541 gtcccaactg aatacaaaaa agtaagaatc gttattgacg aaaaataaga agggcgggac
    15601 ggatgtctcg cttttttgtg tggaatagaa gatatagtaa aatgaaacaa gaacaggaca
    15661 aatcgatcag gacagtcaaa tcgatttcta acaatgtttt agaagtaaag gtgtactatt
    15721 ctagtttcaa tatactataa aagattgaac gtttaggcaa ttccaagact acttaatcga
    15781 acattaacag cagtctcgga ctgatgaagg tctctttatg aagttagtgg aggaagtcgg
    15841 agaagtagcc gaggttttga ataggcgctc tggtcgaaaa gaagggatag gattcgaacg
    15901 aggaacttgc cagagaactg gctgatatca tttactacac cgtcgcaatc gtagctatta
    15961 atcatattgc ccttaccaag actgtctttg agaaagacaa aaccgcagcc gttaattacc
    16021 aacataaaca taatttggaa ggattcttga agggaaagta gagatgttgt ccccttcctc
    16081 caacttgaga aaatccttgg aacgtgataa aataaaggat aatgattttt aataattcat
    16141 tatatagaat gagtggattt ctttatgaag aggtttgaag tatctacaga aattggtagc
    16201 ctctctgtta cttatcaaaa gcaaaagaaa gtgctagttt gtctaaacgg agcaggtttg
    16261 ctaccaagtt atgaaaattt ttcacttata cttgaaaaac ctcctcctac aattggttat
    16321 ttgacaattg attttccgaa cacaggtagg agtccgattc atgaccaagc tggaaaaaat
    16381 ctagataatc ttgcagatgc ggtttatgaa gtacttgaag aattaggaat ttctgaatat
    16441 atactttgtg cacatagttg gagtggaatt ttagcttgca aattactcga gaaaccaatt
    16501 aagcgtcaga ctttagtagc aattgaaccg acaactaaaa aagtcatgtt tgctgatttt
    16561 tcagaaaatc cttatccaga aatggaagag cagatgaggc tgattgacga gtgtggtcct
    16621 gaactttatt ttaagaactt aactcgagca acatttagtc ctgaaacgaa taaaaaaatc
    16681 tgggaattaa tgcaagaaaa aggcttagag ttggaaaatc aagatccaga atttcagata
    16741 tctggagaga ttactgagga agattttgag gatgtatcta tagaagctca tattcctgta
    16801 tttgtttttt gtcagcctta tagagaaaaa gagtacagag aatcagaata ttggacttcc
    16861 aatactaaac tcattttagg agggaatcac cattatttac aatggtcaga atcggaaaaa
    16921 attgcggcta ttattcgaga attgtcagaa taagatggaa aaaaggagat tacaggagac
    16981 aagatgaact actttaatgt tgggaaaatc gttaatacgc agggattaca gggtgagatg
    17041 cgagtcttgt ctgtgacgga ttttgcagaa gaacggttta aaaaaggagc tgagctggct
    17101 ttgtttgatg aaaaagatca gtttgtccaa acagtgacca tcgctagcca ccgtaaacag
    17161 aagaactttg acattattaa attcaaagat atgtaccata tcaatactat cgaaaagtac
    17221 aagggatata gtctcaaggt cgctgaggaa gatttgaatg acctagacga tggtgaattt
    17281 tactatcacg agattatcgg tttggaagtc tatgagggtg atagcttggt tggaaccatc
    17341 aaggaaatcc tgcaaccagg tgctaatgat gtctgggtgg tcaaacgaaa aggcaaacgt
    17401 gatttgcttt taccttatat cccaccagtg gttctcaatg ttgatattcc aaataaacgg
    17461 gtcgatgtgg aaatcttaga agggttagac gatgaagatt gatattttaa ccctctttcc
    17521 agagatgttt tctccactgg agcactcaat cgttggaaag gctcgagaaa aagggctctt
    17581 ggatatccag tatcataatt ttcgagaaaa tgctgaaaag gcccgtcatg tagatgatga
    17641 gccctacgga ggcggtcagg gcatgttgct cagagcacaa cctattttcg attcctttga
    17701 tgctattgaa aagaaaaatc cgcgcgttat tctcctcgat cctgctggaa agcagtttga
    17761 tcaggcttat gctgaagatt tggctcaaga ggaagagcta atctttatct gtgggcacta
    17821 tgagggttat gatgagcgca ttaagacctt ggtaacagat gagatttccc taggcgacta
    17881 tgtcctcact ggtggagaat tggcagctat gaccatgatt gatgctacag ttcgcctgat
    17941 tccagaagtg attggcaagg agtctagcca ccaagatgat agtttttctt caggtctttt
    18001 agaatatcct cagtacacac gtccctatga ttatcgaggc atggtcgtgc cagatgtatt
    18061 gatgagtggc caccatgaaa agattcgtca gtggcgattg tacgagagtt taaagaaaac
    18121 ctacgagcgc agaccggatt tacttgaaca ttatcaactg acagtagaag aagaaaaaat
    18181 gctggcagaa atcaaagaaa acaaagaata aaggagaaac ctatgcaagt aatcaaacgt
    18241 aatggcgaaa ttgctgaatt taatccagat aagatttacc aagccatctt gaaggcagcc
    18301 caaactgttt atgttttgac agatgatttg cgtcaaaatc ttgctcaagt cactaagaag
    18361 gtagttttgg atttacaaga agccaaggtg gaacgtgcga ctatcagtat gattcaatct
    18421 atggttgaac atcgtttatt gggcgcaggt tacattacca ttgcagaaca ctacatttcc
    18481 tatcgtttac aacgtgactt ggaaagaagt ggttatggag atcatattgc agttcattta
    18541 cattttgaac aaattcgcta agaaaaaaga gtgggatgaa gcaactacat ctcactcttt
    18601 cttaaatcta tttttttggg ctgtttggac agttgagggg acaaatcgca ggcgttgaat
    18661 atcgcttata cggataatcc ggctcacatt tttggcaaaa tttttcacga taatttgctg
    18721 gccttgctga tcatatttga tgatatcacc tgtaaaactt gtctcagaca agataaggtg
    18781 aacagcggtt tgtttctgga tagcctcttc aatagtggct gtaagggagt tggtttcagt
    18841 ctggtcaggt tggtcatgtc catttaaaaa atcatgtatt ttatctagaa cttgctggaa
    18901 tttatgtctc atattggcct cccttctttt atatcaatat tatataaaat tttcctaaaa
    18961 tctacaagat tttacgaata aactaatgaa actaaaaaag gagaagaaaa tggcagaatt
    19021 tacatttgaa atcgaagagc acttgttgac tctttctgaa aacgaaaaag gttggaccaa
    19081 ggaaattaac cgtgtgagct ttaatggtgc ccctgcaaag tttgatattc gtgcttggag
    19141 tccagaccat actaaaatgg gcaaagggat tactctctca aatgaagaat ttcaaacgat
    19201 ggtggatgcc tttaaaggca actaatactc ttcgaaaatc tcttcaaacc gcatcagctt
    19261 tgccttgccg tatgtatggt tactgacttc gtcagtttca tccacaacct caaaacagtg
    19321 ttttgagcag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataagtt
    19381 ttcaaaataa aaaaaaggat attgagtcat gataactcgg tatccttttt tagttagtaa
    19441 agtaagtctg atttttaagg cgttgaagta ggggacggct aataaaacta atagccagaa
    19501 gtttgccaag gtctggaatg ataaagggaa gaacacccac aacaagagct ttttcaaatg
    19561 ccattccagc taggaaatgc aagctgagaa tcccgccgac aaagacaagg gcatcaccca
    19621 agaggtttgc aagaaaaatc ttaacaacac cactcttgct gttggttaga gaggaagtaa
    19681 gtccagagta aacgagataa aaccaaagat agcctgcagt agggccaact aaagcctgaa
    19741 aaccagctcc acctcctgca aagacaggaa gaccgatagc acctagaaga agatagagtc
    19801 cagcagaaag tacagcctct ctcggtctaa agacagtagc aatcaagccg attgcaaagt
    19861 tttgcagagt gaagggaaca ggtccaattg gaagactgat ttgtgccaaa acagcaatga
    19921 gagcagctcc aatagcaggg atagcataaa cgtgagcttt tttcaaaata gttaacctct
    19981 tttctaatgt atagtaacta ttatactcat ataggagtcg atgtcaacca ttttttaaaa
    20041 acaaggtaac aaaaactgta acagccgatt ttcgtgacta gtcaagttgg ataatttttt
    20101 tgttgattcg aaaatcatta aaaaagaagc agacatattt atatcagctt cttttgtttc
    20161 gattaacgca tggttacaaa ttcttcagat gcagttgggt gaattgccac agttgcatca
    20221 aagtcagcct tggttgctcc cattttgata gcaacggcaa atccctgaat catttcatca
    20281 acgccgtagc caattccatg aagtccgaca actttttctt ctgaaccagc tgttatgagt
    20341 ttgaaacggg attcttgacg gttgcgagtg caagcagagt acatagatgc aaagcttgat
    20401 ttataaacct tgatttggtc ttgaccgtat tctttaatag cttgctcttc tgtcaaacca
    20461 acagttccga tagcagggtg tgaaaagaca acagttggaa tagttgagta atccattttt
    20521 gcagtagttt ttccgttaaa gagacgttca gatagggtac gtccggcctt tattgcaact
    20581 ggagtcagtt ctttctcgcc cgttacatca cctagagcat agattccctc aacaacagta
    20641 ttttggtatt catccacttg gataaagcca cgttcgttca gagtcactcc agctttttca
    20701 agttgcaagc ccttaacgtt tggacggcga cctgtagccc agataacttg gctagctgtg
    20761 tgactagtac catcttcgaa atgaatggta atgccgtcag tagttttttc taacttgaca
    20821 gggactttgt gagtgtgaag tggtaagttt gttctttcca tttccttgac caaaccttca
    20881 acgatgtagg aatcaaaacc acgtaaagga cgatcgcggc gaacaaagag atctgtcttg
    20941 acaccaaaag tgtggagtac gccagctaat tcaacggcga tataaccagc gcctagaatg
    21001 gcaattgact ctggaagttc ttcccaggca aatacatcat cagaagagcc acctagctca
    21061 gcaccaggaa tattaggaat acttggatgg gcacctgtag caatcacgat atgtttagca
    21121 cgaatcagtt caccatttat gcttacagta tgagaatcta caaattcagc atgaccttca
    21181 atcaagtcta caccgttgcg tttaaaacta ccatcataag aagaacgagc gcgatcaatg
    21241 taggcttcac gattgcgacg tagggttgca aagttaaagt taagatcagt agtcttaaag
    21301 ccgtagtctt ctccaaattg atggaaagtc tcagcgattt gcgccccgta ccacatgatt
    21361 tttttaggaa cacaaccgac gttgacacag gttccaccta atttcttttc ctcaataacg
    21421 gctgctttgg ctccatgttc accagcacgg ttcatggtag cgattcctcc gctacctcca
    21481 ccgatagcaa tgatatcata ttctctcatt gaaaactcct ttgtaatctt ttaaatagta
    21541 gagtgtcttt tttgatagtt atattttatt ctttttttga tttgattgca aaaatctgct
    21601 acgcacaaaa aattgcaaaa aagcttgcca aataagtcaa gatagtgtat tatatatcta
    21661 gtacaataga aaagtaaaat tccgaacgat tgtttaatcc tgaactgcca ttatttccat
    21721 ctgaactgca ttgtttgctg gagggaaatt tggtataata gcaaagtatt gtaaaaaaga
    21781 aaaggagttt cattatgaag aaaaagaatg gtaaagctaa aaagtggcaa ctgtatgcag
    21841 caatcggtgc tgcgagtgta gttgtattgg gtgccggggg gattttactc tttagacaac
    21901 cttctcagac tgctctaaaa gatgagccta ttcatcttgt tgttgccaag gaaggaagcg
    21961 tggcatcctc tgttttattg tcagggacag taacagcaaa aaatgaacaa tatgtttatt
    22021 ttgatgctag taagggtgat ttagatgaaa tccttgtttc tgtgggcgat aaggtcagcg
    22081 aagggcaggc tttagtcaag tacagtagtt cagaagcgca ggcggcctat gattcagcta
    22141 gtcgagcagt agctaaggca gatcgtcata tcaatgaact caatcaagca cgaaatgaag
    22201 ccgcttcagc tccggctcca cagttaccag cgccagtagg aggagaagat gcaacggtgc
    22261 aaagcccaac tccagtggct ggaaattctg ttgcttctat tgacgctcaa ttgggtgatg
    22321 cccgtgatgc gcgtgcagat gctgcggcgc aattaagcaa ggctcaaagt caattggatg
    22381 caacaactgt tctcagtacc ctagagggaa ctgtagtcga agtcaatagc aacgtttcta
    22441 aatctccaac aggggcgagc caagttatgg ttcatattgt cagcaatgaa aatttacaag
    22501 tcaagggaga attgtctgag tacaatctag ccaacctttc tgtaggtcaa gaagtaagct
    22561 ttacttctaa agtgtatcct gataaaaaat ggactgggaa attaagctat atttctgact
    22621 atcctaaaaa caatggtgaa gcagctagtc cagcagccgg aaataataca ggttctaaat
    22681 acccttatac tattgatgtg acaggcgagg ttggtgattt gaaacagggt ttttctgtca
    22741 acattgaggt taaaagcaaa actaaggcta ttcttgttcc tgttagcagt ctagtaatgg
    22801 atgatagtaa aaattatgtc tggattgtgg atgaacaaca aaaggctaaa aaagttgagg
    22861 tttcattggg aaatgctgac gcagaaaatc aagaaatcac ttctggttta acgaatggtg
    22921 ctaaggtcat cagtaatcca acatcttcct tggaagaagg aaaagaggtg aagactgatg
    22981 aagcaactaa ttagtctaaa aaatatcttc agaagctacc gtaatggtga ccaagaattg
    23041 caggttctca aaaatatcaa tctagaagtg aatgagggtg aatttgtagc catcatggga
    23101 ccatctgggt ctggtaagtc cactctgatg aatacgattg gcatgttgga tacaccaacc
    23161 agtggagaat attatcttga aggtcaagaa gtggctgggc ttggtgaaaa acaactggct
    23221 aaggtccgta accaacaaat cggttttgtc tttcagcagt tctttcttct atcgaagctc
    23281 aatgctctgc aaaatgtaga attgcccttg atttacgcag gagtttcgtc ttcaaaacgt
    23341 cgcaagttgg ctgaggaata tttagacaag gttgaattga cagaacgtag tcaccattta
    23401 ccttcagaat tatctggtgg tcaaaagcaa cgtgtagcca ttgcgcgtgc cttggtaaac
    23461 aatccttcta ttatcctagc ggatgaaccg acaggagcct tggataccaa aacaggtaac
    23521 caaattatgc aattattggt tgatttgaat aaagaaggaa aaaccattat catggtaacg
    23581 catgagcctg agattgctgc ctatgccaaa cgtcagattg tcattcggga tggggtcatt
    23641 tcgtctgaca gtgctcagtt aggaaaggag gaaaactaag atgcagaatc tgaaatttgc
    23701 cttttcatct atcatggctc acaagatgcg ttctttgctt actatgattg ggattattat
    23761 cggtgtttca tcagttgttg tgattatggc tttgggtgat tccctatctc gtcaagtcaa
    23821 taaagatatg actaaatctc agaaaaatat tagcgtcttt ttctctccta aaaaaagtaa
    23881 agatgggtct tttactcaga aacaatcagc ttttacggtt tctggaaagg aagaggaagt
    23941 tcctgttgaa ccgccaaaac cgcaagaatc ctgggttcaa gaggcagcta aactgaaggg
    24001 agtggatagt tactatgtaa ccaattcaac gaatgccatc ttgacctatc aagataaaaa
    24061 ggttgagaat gctaatttga caggtggaaa cagaacttac atggacgctg ttaagaatga
    24121 aattattgca ggtcgtagtc tgagagagca agatttcaaa gagtttgcaa gtgtcatttt
    24181 gctagatgag gaattgtcca ttagtttatt tgaatctcct caagaggcta ttaacaaggt
    24241 tgtagaagtc aatggattta gttaccgggt cattggggtt tatactagtc cggaggctaa
    24301 aagatcaaaa atatatgggt ttggtggctt gcctattact accaatatct cccttgctgc
    24361 gaattttaat gtagatgaaa tagctaatat tgtctttcga gtgaatgata ccagtttaac
    24421 cccaactctg ggtccagaac tggcacgaaa aatgacagag cttgcaggct tacaacaggg
    24481 agaataccag gtggcagatg agtccgttgt atttgcagaa attcaacaat cgtttagttt
    24541 tatgacgacg attattagtt ccatcgcagg gatttctctc tttgttggag gaactggtgt
    24601 tatgaacatc atgctggttt cggtgacaga gcgcactcgt gagattggtc ttcgtaaggc
    24661 tttgggtgca acacgtgcca atattttaat tcagtttttg attgaatcca tgattttgac
    24721 cttgttaggt ggcttaattg gcttgacaat tgcaagtggt ttaactgcct tagcaggttt
    24781 gttactgcaa ggtttaatag aaggtataga agttggagta tcaatcccag ttgccctatt
    24841 tagtcttgca gtttcggcta gtgttggtat gatttttgga gtcttgccag ccaacaaggc
    24901 atcgaaactt gatccaattg aagcccttcg ttatgaatga gatcaacaag atggacactc
    24961 gtctatcttg tttttgtatg gatttcccta tcgaaattca ttaaaaatgt gatataatga
    25021 agtgttagaa aaacaggttt catttgcctg gaaaggaaaa attatgtctg aaaagaattt
    25081 ttatattaca acgccgattt actatccatc tgggaaactt catatcggtt ctgcctacac
    25141 aactatcgca tgtgatgtcc tagcacgtta caaacgcctg atgggctacg atgtatttta
    25201 tctgacaggt cttgatgaac atggtcagaa aatccagcag aaagcggaag aagctggtat
    25261 tacacctcaa gtctatgttg atagaatggc ggttggagtt aaagaactct ggcaattact
    25321 agatatctca tacgataaat ttatccgtac aactgatgac taccatgaaa aagttgtcgc
    25381 acaggtcttt gaacgcttac ttgctcaaga tgatatctac ttgggtgaat actctggttg
    25441 gtattcagta tcagacgaag aattctttac agaaagccag ctggcagaag ttttccgtga
    25501 tgaagctgga aatgtgactg gtggtattgc tccatcaggt catgaggttg aatgggtttc
    25561 tgaagaatca tacttccttc gccttagcaa ataccaagac cgtttggtcg aatttttcaa
    25621 agctcatcct gaatttatca cgccagatgg tcgccttaat gaaatgctac gcaacttcat
    25681 cgagccaggc ttggaagatt tggcggtatc tcgtacaacc tttacatggg gagtgcctgt
    25741 cccatcaaat ccaaaacacg ttgtctacgt ttggattgat gcccttctta actatgcgac
    25801 agctcttggc tacgctcaag acgaacatgg taactttgac aagttctgga atggaacagt
    25861 cttccatatg gtaggaaaag atatccttcg cttccactct atctactggc caatccttct
    25921 tatgatgttg gatgttaaat tacctgatcg tttgattgcc catggttggt ttgtcatgaa
    25981 agacggaaaa atgtctaagt caaaagggaa tgtcgtttac cctgaaatgt tggtagagcg
    26041 ttatggacta gatccacttc gttactacct catgcgtaac cttccagttg gttcagacgg
    26101 aacctttact cctgaagact atgtcggccg tatcaactat gaattggcta atgaccttgg
    26161 gaacctcctt aaccgtacgg tttccatgat taataagtac tttgatggac aaatccctgc
    26221 ctatgtagaa ggtgtgactg aatttgatca tgttcttgct gaggttgcag aacaatcaat
    26281 cgcagacttc catacacaca tggaagcagt tgactatcca cgtgcgcttg aagcagtctg
    26341 gactctgatc tctcgtacca ataaatacat cgatgagacc gcaccatggg tcttggccaa
    26401 ggatgaagct cttcgtgacc aattggcaag tgtcatgagc cacttggcag ccagcattcg
    26461 tgtagttgct cacttgattg aaccatttat gatggaaact agtcgtgcag ttttgactca
    26521 acttggtttg gaagaagttt ctagtcttga aaacttgagt ttggctgact tcccagcaga
    26581 tgtgactgta gttgccaaag gaacacctat ctttccacgt ctaaatatgg aagaagaaat
    26641 cgcctatatc aaggaacaaa tggaaggcaa taaaccagca gtcgaaaaag aatggaatcc
    26701 ggacgaagtt gagctcaaac taaacaagga tgaaatcaag tttgaagact ttgacaaggt
    26761 tgaaatccgt gtcgcagaag tcaaagaagt gtctaaagta gaagtttcag ataagttgct
    26821 tcaattccgc ttggatgctg gtgatggaga agatcgtcag attctttcag gaattgcaaa
    26881 atactatcca aatgaacaag aattggtcgg caagaaagtt caaatcgttg ctaacctcaa
    26941 accacgtaaa atgatgaaaa aatatgtcag ccagggtatg attctctcag ctgaacatga
    27001 tggcaaatta acccttctca cagttgatcc agctgtacca aatggaagtg tgattgggta
    27061 aaagtagaca ggactagttc aggctagttc tgttttttta tttatactca atgaaaatca
    27121 aagagcaaac taggaagcta gccgcaggt
//
LOCUS       DAVDWM010000025        27117 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVDWM010000025 DAVDWM010000000
VERSION     DAVDWM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.025"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(155..1141)
                     /locus_tag="VUW49_001259"
     CDS             complement(155..1141)
                     /locus_tag="VUW49_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7981178.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1151..1951)
                     /locus_tag="VUW49_001260"
     CDS             complement(1151..1951)
                     /locus_tag="VUW49_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET7981179.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQTPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYYK"
     gene            complement(2204..3046)
                     /locus_tag="VUW49_001261"
     CDS             complement(2204..3046)
                     /locus_tag="VUW49_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7981180.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3048..4340)
                     /locus_tag="VUW49_001262"
     CDS             complement(3048..4340)
                     /locus_tag="VUW49_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7981181.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4451..5722)
                     /locus_tag="VUW49_001263"
     CDS             complement(4451..5722)
                     /locus_tag="VUW49_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7981182.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6272..7789
                     /gene="malQ"
                     /locus_tag="VUW49_001264"
     CDS             6272..7789
                     /gene="malQ"
                     /locus_tag="VUW49_001264"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7981183.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7815..10073
                     /gene="glgP"
                     /locus_tag="VUW49_001265"
     CDS             7815..10073
                     /gene="glgP"
                     /locus_tag="VUW49_001265"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET7981184.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVCSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11118..11690)
                     /locus_tag="VUW49_001266"
     CDS             complement(11118..11690)
                     /locus_tag="VUW49_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981185.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12313..13161)
                     /locus_tag="VUW49_001267"
     CDS             complement(12313..13161)
                     /locus_tag="VUW49_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7981186.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13313..13591
                     /locus_tag="VUW49_001268"
     CDS             13313..13591
                     /locus_tag="VUW49_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981187.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13593..15656
                     /locus_tag="VUW49_001269"
     CDS             13593..15656
                     /locus_tag="VUW49_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7981188.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15734..16990)
                     /locus_tag="VUW49_001270"
     CDS             complement(15734..16990)
                     /locus_tag="VUW49_001270"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7981189.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAIRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17132..19597
                     /locus_tag="VUW49_001271"
     CDS             17132..19597
                     /locus_tag="VUW49_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET7981190.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKAGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19662..20573
                     /locus_tag="VUW49_001272"
     CDS             19662..20573
                     /locus_tag="VUW49_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7981191.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSFVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20803..21501
                     /gene="dapD"
                     /locus_tag="VUW49_001273"
     CDS             20803..21501
                     /gene="dapD"
                     /locus_tag="VUW49_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET7981192.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATSVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21569..22699
                     /locus_tag="VUW49_001274"
     CDS             21569..22699
                     /locus_tag="VUW49_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET7981193.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22711..23250
                     /locus_tag="VUW49_001275"
     CDS             22711..23250
                     /locus_tag="VUW49_001275"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7981194.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQFIDQTLTERLLHHPFYQEAKVI
                     ATYLSFPHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTSFGLLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23234..23911
                     /locus_tag="VUW49_001276"
     CDS             23234..23911
                     /locus_tag="VUW49_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7981195.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(23978..24877)
                     /gene="galU"
                     /locus_tag="VUW49_001277"
     CDS             complement(23978..24877)
                     /gene="galU"
                     /locus_tag="VUW49_001277"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET7981196.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(24899..25915)
                     /locus_tag="VUW49_001278"
     CDS             complement(24899..25915)
                     /locus_tag="VUW49_001278"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7981197.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKQLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26127..26507
                     /locus_tag="VUW49_001279"
     CDS             26127..26507
                     /locus_tag="VUW49_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7981198.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(26681..>27117)
                     /locus_tag="VUW49_001280"
     CDS             complement(26681..>27117)
                     /locus_tag="VUW49_001280"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981199.1"
                     /translation="KVHLTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSS
                     TMIKNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHIL
                     KFSSLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
BASE COUNT         7912 a         4993 c         5936 g         8276 t
ORIGIN      
        1 ctttgatttt cattgagtat aaaaagatct tgggaaaaga ctcaatttca gtagaaaagg
       61 aagtaaatct tcccacatta aaacgcatag tatcaagttt tttcaatacc tgatagtatg
      121 cgtttttgct tttaacgact ttttgcccag tctcttattt ttcgataatt ttgtgggcga
      181 tcaattgacg gtaacaaatt tgtttattgt ttttattatc attaataatc tggagaatag
      241 tttcaaggga aacacgacca agctctaaac tattgatatc gacataggct gccaagttga
      301 gcttgggatt aaccgagtca aagctgagaa cagggacatc cagctggtgt ttggcaatat
      361 agttacaaac accttcagct aggaggctat cggttgtgat gatagcatca atttgtggat
      421 cgtgcttgaa taatcgcttg ctaaatttat agcccttttc ttccagaaac tcgtcggcaa
      481 agtagatgcg attgttgtca gtggtaagtt tgtaatgttt aagcgcctgt tcatagcctg
      541 ttaaacggtc tttggtcacg aagagctttt tacttcctcc gataaaggca atgcgtttgc
      601 agcctttttt gatgaaatat tcagtcgcat caaaaccagc ttgaacattg tcgttgtcga
      661 caagtgggat gaaaggagat agagatttac ctaagataag gaaggggaac tgttcttctg
      721 cgacgagttt tacgagaggg tcttcttctt gggcatagag aaagattagc ccatctacac
      781 gcttgccgta gaccatttgt gaaatagcgt tgagacgctc cttctcatct ttccctgttg
      841 ctatctgaat ggcatagtgg ttttcagatg cgacttgaga gatgccacgt agaaccgatg
      901 gaaagaaagg attctggtag aaggcgtctg agtcatcagg aagaactaat ccgataacct
      961 gagtatagct gcttaccaag ctacgagcgt tgaggtttgg gtggtagttg agttccttca
     1021 tagctttgcg aacgcgtttt tttgtttcgt cgctaatggt tgatttattt tgaataacac
     1081 gggttacggt tgaaggcgaa acaccagcag ccttggccac gtctttaatc gtaacgggca
     1141 taaaaatctc ctatttatag taatttggat cacggaaatg tgttttataa aagatagtga
     1201 ccagatacag aacaaaaaga atattttgta cagtaatcag ggttgtcata ttttggccaa
     1261 ataatcccaa aataagtgta atcagagtcg gcaatcctaa acagttcaag ataaagtgat
     1321 agcactcttt aaaggttcta aatgaaaaga ggcgtgattt cttagtgata taaaggagaa
     1381 gactagctcc tagagagacg ataaagaaat tcaaaccaaa gaggaagctc gcaccgagaa
     1441 ctaggaagag gctgatatag acacgatttt gttggtacca gtctttagaa attgcttggg
     1501 tcaagctgtc tttgcttttg aaactctcag tctgaatggc tcggtaagag atgcgggtca
     1561 gctccttgct ttccttgctg atgactagct catttgtatc gaaatgcagt tgcaagtcct
     1621 taggtaattc cttgatttga cttggaccaa tcacaacaga aggggtttga ctagctgttc
     1681 cagtataagt taatgtgcca tcgacaattg tagcatgttc agagagatcc tggacaacct
     1741 catttgtcag aggttcatag acattatcga taaaagtttc tagcggatag gtctcctggg
     1801 agctgttttg gatagcaatg ggtaccatag acaagctgat aaggaagata ctggtaaaga
     1861 gaagttgaaa ccagttgagc tcgaaacgtt tggacagggg cttacgaaac ccccaaatac
     1921 ttgaaaaata ggaaaatgga tatggaagca taggtatctt tctaaaaggt gttttctata
     1981 tgagtattat tatatagaga aatgcgcttt atttcaagtc taggagaaaa attgcttgct
     2041 acaaggatat tatttacagt gacaattgct aataatcaat gaaaatcaaa gtgcaaacta
     2101 gccacaggtt gctcaaagca caactttgag gttggagata aagctgacgt ggtttgaaga
     2161 gattttcgaa gaatataaaa tgaaaaaggg tggcggggat aaattatccc ttgtcgccac
     2221 cacttgtaag tcctgaaaca aagttctttt gtaggaagaa gaagagaata cagattggaa
     2281 gggcgatgag gatagcacct gctgagaagt aggcaatctt caagtttttc gcattgttaa
     2341 cgaaggtttg gagacctacg gcaacagtaa agtattcttt ctcacgaagc aagaaactag
     2401 agaggatgta gtccccgaaa ggtcccatga aggcccagag agcttgtacg gcaaccattg
     2461 ggcgaacaag tggtagaaca atttgccaga agcggcggaa gtgtcctgca ccgtctagtt
     2521 ttgcagattc gtctaaagac attggcactg tatcgaagta gcctttcatg agccaagcat
     2581 tcatcgggat accaccacca acgtagagga agatgaggaa ccagttgtgg ttaagggcgt
     2641 tcaacataag cgccataacg aagaaggctg tcaaagcggc catagttggc accatttgga
     2701 tgatcaagaa gaagaccaaa ctttgtttac gagccaagaa gttgtaacgg ctgtaagcat
     2761 aaccagcaag tacgatgata cttgtttgaa cagccatggt aattaaggcg ataatcaaag
     2821 tgttgaggta ccaagtaccg tacaaggttt cagtgaagag gcctttaaag ttatcaaaat
     2881 tgaggtcgat attagtatct agtttaaagg ctgagacgtt acctgcttta aaggctgaca
     2941 taatggtaat caacagtgga tagataatta caattgatag accaatcagg taaaggtaag
     3001 taaggctttg agtcagtcta cgtttgagtt taattgagtt attcatctta gacgtcctcc
     3061 atatcaaatg cgtgtagttt cttgaatgcg atcatagaga ttgagatgac aatgatagag
     3121 ataatcaagg taacagctgc cgccattgag tattgaggag atgtacctgt tgtcaaacgg
     3181 tagatccatg agatcaagat atcggttgaa ccagctccac ctccgacact accaggtcct
     3241 ccaccattga agaggtacat gatagagaag ttgttaaagt tgaaggtgta ttggctaatc
     3301 aaagtaggtg ccgcaacagc caaaatcatt gggaaagtga tgttgcggaa tttttgccaa
     3361 gcgttggcac cgtcaatata agctgcttcg taaaggtcgt taggaataga ttgcaagata
     3421 cccaaagtca aaacgtagat gtatgggaat ccgagccaac cttgcatcat aatcaaggca
     3481 atcttagtcc aagttgggtc tgttttccaa ggaataagag ctccatcaag gaaaggaagg
     3541 aatttagcca agattggcaa tacttgagtg ttgatagcac cgacactatc gttaaacata
     3601 tttgagaatg tcaagatagt gatgaaggct gggacagccc aaggaagaag gaaaataaca
     3661 ccaaagatac gttttccttt gataaatggt tggttggcaa tgatagctgt gaagatacca
     3721 attacgattt gtaaagtaga agctgccaaa gcccaaatga tagtccaaga aagaacagaa
     3781 ccaaaggcag aacggaaggt actcaagctc caaatgtttg taaagttggt caaaccaacc
     3841 cagtccaaca acttgtttgg tggcaagtgt tggaagtcgt agttggtaaa ggcgatcatc
     3901 aaggttacga taactgggaa gataatcgcg aaggtcatgg caacataaga tggaatgatc
     3961 aagaggtaag ggaagccatt ttcataaatc cctttgatca tgtctttgag tgtgcgtgga
     4021 actggaattc cattgttaat gcgttttgca atcgtatgtg catctttgat atttgagaaa
     4081 taaaagagta cataaacgat tacaaagatt agatggaagg caccacgaat cagcataaag
     4141 agggaattat cacgacctgg tttgtcacca agagtgatga ggttgctcaa ttcaggggtt
     4201 gcaagtgcta ggaagtaaag gacaaatacg atggttacac caaggaagat aaaacctttg
     4261 gctttttgtt tattgtaaat ctgtcctaac ccaggaatga tagacagcag ggctgcttta
     4321 ctaggttgtt gcttttccat attaactcct ttcataggat actacttttt tataagaaag
     4381 ctaaattatt tgtgtctcta ttgataaatt caaaggggga tttgatttcc accccccttg
     4441 aacaaatttt ttattcacca aatttttgtt tgattgtttc tttgatcaat gttacagcat
     4501 cgttagcagc tgttttagca tctttttgac cacttacagc atcaaagagc atatttttcg
     4561 ctggatccca aactgcagac atttgagaga tgtttggcag tggttgagtg ttcttgaact
     4621 gtttgataac agctgttgtc aactcatcgt ttttaccttc agcgtatgaa cgagcctcag
     4681 tattagctgg gatttcgtta gtcttatcat ataatacttt ttgttgttca gttgcaacaa
     4741 ggaagtctac aaatttttga gaagcttcaa ggttcttaac ggcttgagga atgacccaag
     4801 ctttaccacc accgaatgca gcatattctt ttccatttgg aagagttggg atagttgcaa
     4861 ctccgtagtt tactttagca tctttaaagg cttgagcttt ccaaggtccg tcgatgatag
     4921 cagctgtttt accttcttgg aattgagttt ggattaagtt tccagcacct tctgtatctt
     4981 gcataccttt aggccatttt tcgtaccaag atttagcgta gttgatacct gcgatagaac
     5041 cgtcgtttgc aagaccgatg tctttagcgt ctttaccgtt ttggccaaag acgtaagcac
     5101 cgttaccggc aagaagtcca tatgtatagt agaagtttgt ccagtcagct aggaaggcag
     5161 tagttttacc atcttcacca gcgaatgcgt atttgctatc tttagcaagg ttttccaagt
     5221 cagcaaatgt ttttggagca tctttcacca agtctttgtt gtagtacata acaagtgact
     5281 cgataacggc aggagcaccg taaactttac cattagcagc tgttacaaga gatttagttg
     5341 tgtcgtctgt tttagcacca tcgctcaatt tcacttctga aagttgtccg tcagaaccaa
     5401 ggctacctac acggtcgtat ggagccatca taacatcagg gacattacca gattggttgt
     5461 caagagaaag tttatcaaga cctcctagag catcaccagt tttaagagtg acttttactc
     5521 cagcttcttt ttcataagct ttagcaacct cttcaatata gctcttatat ccctcgtcta
     5581 catatacagt gagttctttg acttcagatg aaccagaatc agcaggctta tcagcagttt
     5641 tgcttccgca agctaccaaa agcaagctag caagtgtagc agttccaagc accgcagtgc
     5701 tcttcataaa tttagatgac atagtgtatt cctcccaaag aatagcaagt tttattgata
     5761 aggaaacgca aacgttttcc tttatgagct tagtatagca caaatagaaa acggttgcaa
     5821 gtattttttg taaaaatttt aaaaaatttt taagcttgat ttgatagcat aattttgcat
     5881 tttaatagaa gaaaagtatg aaatagataa gaatacagta tttttaataa aaatatatgg
     5941 aatctgtcta aagagggaaa tcaggagggg ctacctccct gtttctaatc cctatctatt
     6001 ttctatagac gtttgtgctt tgttacatat aaattcaagc tccctaagga ggttttgtac
     6061 tttgtagact gtctcgtgag agtcaaacct taggattaga tagttttaag ttatctttgc
     6121 taaaatgagt tagtctcctt aaaaataatc aaaaaagttt tttaaaaacg cttgcaatta
     6181 tgcttgaaaa ggagtatact tataagtaac gcaaacgttt gcgtctgaaa aatacgcaac
     6241 gttccattat tttaacacac gaggtgctat tatgaaaaaa cgtcaaagtg gtgtgttgat
     6301 gcacatctct tctcttccag gagcttacgg aatcggatca tttggtcaaa gtgcttacga
     6361 cttcgttgat ttcttggtcc gtacaaaaca acgttactgg caaatccttc cattaggagc
     6421 aactagttac ggggattctc cttaccaatc tttctcagcc ttcgcaggaa acactcattt
     6481 tatcgattta gatatcttgg tggagcaagg tttgttggaa gcaagtgacc ttgaaggagt
     6541 tgactttggt agcgatgcgt ctgaagttga ctatgctaaa atctactatg cacgtcgtcc
     6601 tcttttagaa aaagcggtga agcgtttctt tgaagtcgga gatgttaaag attttgagaa
     6661 atttgctcaa gacaaccaat catggcttga gctctttgct gagtatatgg ctatcaaaga
     6721 gcattttgac aatcttgctt ggactgaatg gccagatgca gatgctcgtg ctcgtaaagc
     6781 ttcagcactt gaaagctatc gtgagcaatt ggcagacaag ttggtttacc accgtgtgac
     6841 tcaatacttc ttcttccaac aatggttgaa attgaaagct tacgctaacg acaaccacat
     6901 cgaaatcgtt ggggacatgc caatctacgt agcggaagat tcaagtgata tgtgggcaaa
     6961 tccacatctc ttcaaaacag atgtcaatgg taaggctact tgtatcgcag gatgtccacc
     7021 agatgagttt tctgtaactg gtcagctttg gggtaaccca atctatgact gggaagcaat
     7081 ggacaaagac ggctacaaat ggtggattga acgcttgcgt gaaagcttca aaatctacga
     7141 tatcgttcgt atcgaccact tccgtggctt cgaatcttat tgggaaatcc ctgctggttc
     7201 cgatacagca gcacctggtg agtgggtgaa aggtccaggc tacaagcttt ttgcagccgt
     7261 taaggaagaa cttggtgagc taaacatcat cgcagaagac cttggcttca tgacagatga
     7321 agtgatcgaa ttgcgtgaac gtactggctt cccaggaatg aagattcttc aatttgcctt
     7381 caacccagaa gacgaaagca ttgatagccc acacttggca cctgctaact cagttatgta
     7441 cacaggaaca cacgataaca atacggttct tggttggtac cgtaatgaga ttgatgatgc
     7501 gactcgtgag tacatggctc gttacacgaa ccgtaaagaa tacgaaacag tggtacacgc
     7561 tatgcttcgt acagtatttt catcagttag ctttatggca attgcgacta tgcaagattt
     7621 actagaattg gatgaggcag ctcgtatgaa cttcccatct acccttggtg gaaactggtc
     7681 ttggcgtatg actgaagatc aattgacacc agctgtcgag gaaggtttgc ttgacttgac
     7741 aacaatttat cgccgaatta atgaaaattt ggtagattta aagaaataag acaataatca
     7801 ggagacaact aaacatgtta tcactacaag aatttgtaca aaatcgttac aataaaacca
     7861 ttgcagaatg tagcaatgaa gagctttacc ttgctcttct taactacagc aagcttgcaa
     7921 gcagccaaaa accagtcaac actggtaaga aaaaagttta ctacatctca gctgagttct
     7981 tgattggtaa actcttgtca aacaacttga ttaaccttgg tctttacgac gatgttaaaa
     8041 aagaacttgc agctgcaggt aaagacttga tcgaagttga agaagttgaa ttggaaccat
     8101 ctcttggtaa tggtggtttg ggacgtttgg ctgcctgctt tatcgactca attgctactc
     8161 ttggtttgaa tggtgacggt gttggtctta actaccactt tggtcttttc caacaagttc
     8221 ttaaaaacaa ccaacaagaa acaattccaa atgcatggtt gacagagcaa aactggttgg
     8281 tttgctcaag ccgtagctac caagtaccat ttgcagactt tactttgaca tcaactcttt
     8341 acgatattga tgttactggt tatgaaacag cgactaaaaa ccgcttgcgt ttgtttgact
     8401 tggattcagt tgattcttct attattaaag atggtatcaa ctttgacaag acagatatcg
     8461 ctcgcaactt gactctcttc ctttacccag atgatagtga ccgtcaaggt gaattgctcc
     8521 gtatcttcca acaatacttc atggtttcaa acggtgcgca attgatcatc gacgaagcaa
     8581 tcgaaaaagg aagcaacttg catgaccttg ctgactacgc agttgtccaa atcaacgata
     8641 ctcacccatc aatggtgatt cctgaattga ttcgtctttt aactgcacgt ggtatcgagc
     8701 ttgacgaagc aatctcaatt gttcgtagca tgactgccta cactaaccac acaatccttg
     8761 ctgaagcgct tgaaaaatgg cctcttgaat tcttgcaaga agtggttcct cacttggtac
     8821 caatcatcga agaattggac cgtcgtgtga aggcagagta caaagatcca gctgttcaaa
     8881 tcatcgatga gagcggacgt gttcacatgg ctcacatgga tatccactac ggatacagtg
     8941 ttaacggggt tgcagcactc catactgaaa tcttgaaaaa ttctgagttg aaagccttct
     9001 acgaccttta cccagaaaag ttcaacaaca aaacaaacgg tatcactttc cgtcgttggc
     9061 ttatgcatgc taacccaaga ttgtctcact acttggatga gattcttgga gatggttggc
     9121 accatgaagc agatgagctt gaaaaacttt tgtcttatga agacaaagca gctgtcaaag
     9181 aaaaattgga aagcatcaag gctcacaaca aacgtaaatt ggctcgtcac ttgaaagaac
     9241 accaaggtgt ggaaatcaat ccaaattcta tctttgatat ccaaatcaaa cgtcttcacg
     9301 agtacaaacg ccaacaaatg aacgctttgt acgtgatcca caaatacctt gacatcaaag
     9361 ctggtaacat ccctgctcgt ccaatcacaa tcttctttgg tggtaaagca gctccagcct
     9421 acacaatcgc tcaagacatt atccatttaa tcctttgcat gtcagaagtt attgctaacg
     9481 atccagcagt agctccacac ttgcaagtag ttatggttga aaactacaac gttactgcag
     9541 caagtttcct tatcccagca tgtgatatct cagaacaaat ctcacttgct tctaaagaag
     9601 cttcaggtac tggtaacatg aaattcatgt tgaacggagc tttgacactt ggtactatgg
     9661 acggtgctaa cgtggaaatc gctgagttgg ttggagaaga aaacatctac atcttcggtg
     9721 aagattcaga aactgttatc gacctttacg caaaagcagc ttacaaatca agcgaattct
     9781 acgctcgtga agctatcaaa ccattggttg acttcatcgt tagtgatgca gttcttgcag
     9841 ctggaaacaa agagcgcttg gaacgtcttt acaatgaatt gatcaacaaa gactggttca
     9901 tgactcttct tgatttggaa gactacatca aagtcaaaga gcaaatgctt gctgactacg
     9961 aagaccgtga cgcatggttg gataaagtca tcgttaacat ttctaaagca ggattcttct
    10021 catctgaccg tacaatcgct cagtataacg aagacatctg gcacttgaac taatactctt
    10081 cgaaaatctc ttcaaaccac gtcagcttta tctgcaacct caaagcggtg ctttgagcaa
    10141 cctgcggcta gcttcctagt ttgctctttg attttcattg agtataagat acaaatttat
    10201 actaatacat tttgtaaaaa agcgagtttc gattgaaatt cgctttttta aatgctgtgg
    10261 atttgggtca atcttgtcta aaaataggga aatcctagat acagtgaagg ctttaaatgc
    10321 tggtttttac tgtcttcagc cttatatttt ttcgtagttg gttacctcat atctcttgta
    10381 ttcgcttaca taaagtatta taatataatt gtaggaaaga aggtgttttt atgatataca
    10441 cacttaaatt ggtgttgttt attacctttc ttgtaataag cttgttacct gataagattt
    10501 ttggaaaaaa taaaaaaatt tggaaaatag tttttgcaat attgacggca gtggcagcat
    10561 tgtcatttat gtactaagtt attttaagaa tgtagggaaa taaaccctac attcttttta
    10621 gttttttctg ttttctaaat tctatttatc caagcgcttc aacatttctt gcttcttcgc
    10681 ttcaagttct gcacgctttt cttcgatttc ggcatgtttt ttctcgagtt cagaacaact
    10741 tgcaccattg ctaaattctt ttcgccatca ggagataggg tgagtcgaca tgtctattac
    10801 tcacccaaag cagtcctaca aagcaggaat tttctgttac ttttttggaa atagtaacgt
    10861 ttatacagct ttgacacttc gtatcaaagc gccaaacaca ctccgaggga tttacagaaa
    10921 gcagaaaagg aatgatctgg tataagatca ttccttttct ctctttttct ttaagtaatt
    10981 atatacaatg tacgacgaag tcgtcattgc aatgctgatc caccacctaa agggaacttt
    11041 aaacaacatt gataagataa agaatataaa caacgaaaat acgttatacc caattaattt
    11101 tattgtatat ctcatgatta aaagttaatc cttccgttgt taggaatggc atcattttta
    11161 tcccataatt gtgctaaata agtccccggt gataataaat tcatagcgga ttctaaagca
    11221 acatcattta caaaccaact acctagatat ctagaaattg ctgaacgaat agcacttttt
    11281 gctgcatgtt ttccttttac tttaattaga tttgcaaggc ctgcagtagt tcctcctaat
    11341 gctaaagcta ttgcagtatc taatagagca cccatttgat taactgtaat accttgccaa
    11401 gctgctctaa atggagagta tgtaggtggg attgtataat cgccttgtaa ttgtcggtta
    11461 attacttctt tgatccattg ttgtgagacg tctggatgaa aagattggat ttcgtttgca
    11521 agtgtattga tttgttcttc tgttagagaa gtgacaggtt gaagttccat atttgtttca
    11581 atttgtgata cttgttcaga agcgtataca gctgaaacac ttggaatcgc tgatacaatt
    11641 aacacaattg acgtcaaaaa aaccgaaata aatttcatta atttgttcat gagcttttct
    11701 cctttttatt tgtatctgct tacattttat tatatactgt tattatagtc aagaaaaata
    11761 tgctattatg ttaaaaaaat atttttaaaa atataaatgg acggatttat tttggatttt
    11821 atttgttatt ttgacctgcc tctatattgg taaccatgat ttgtttactc tcaatcatca
    11881 agaattctct tttcgtggta gcgtttgggg tctggtactg gccttatatc acttattgtt
    11941 cattgataag tttgtaattt cgaatcgaaa ataaagatta gaactatgat tgactggcta
    12001 cttttaatat tgattttgga ggaagatttt gtctctatta tttattattt taaatttatt
    12061 tattttgtat aagatctatt ctttgaggcg ggaaaaatcg aaatacttga tttatacggc
    12121 ctatatcata tttggggtaa atgtagtata tggtattcaa tggttattaa gagaactgat
    12181 ttcaactatt tccccttaat gtgtatggaa actataacaa aacgcataag attaaggttt
    12241 gtgtacctgt tcttatacgt ttttgtaata ttgaagattt tcggtaagtt ttataccttt
    12301 gggaagtcta gtttagaatg ctttcccaac caaaatctct gcttcgatgg taatctctgt
    12361 cagttggctc cagtcaatct tgctctggtc aacatgaaag aggagaggag tcatactgag
    12421 tagggcttgg agttgttctg ctgtgatagt cttagtcaga gaggcagttt gactagatag
    12481 gatggtaaag tgttcctgga aatgctcctt gatatcttgg ttagaatact ccttgtttgt
    12541 cagctggtcc tgtactcttt gacggatttc tttgaggtga ttttcagttg ggataacctt
    12601 tatcaagata ccgtctttgg ataaaacgcg acgaaattct ccatagttgg caggtgagaa
    12661 gatatcaagc agaatatcca tgttagcgtc ttttatagga agtcgtgcca agtcgccaac
    12721 gaaccaattg actgcccagt tgggttcact tttagcagcg atttggactg aatctttgga
    12781 gatgtcaaag gcatagaaag ttttttcaga gtgactttct tgtagtttgc gagaatagaa
    12841 tccttcacca caaccgatat ccaaaattgt tgtggtagtt tttgagcttg caagcaagtc
    12901 agatacagca tctaagatag cttggtaaaa gccggcttct aggatttgtt gacggttttg
    12961 aaaattttcc ttgtcgtagt tagcagattg cttgatttga ggtgctagat tgacatagcc
    13021 aaatttcgcc aagtcaaaag aatgacggtt gcagcacttg aaattagtct ctaacagagt
    13081 cagattttct tgacagatag gacaggcaaa ggcagtcgca gaagcaaaac gctgaagttt
    13141 gggcttgaga tttgtattca tagtttaagt gtatcaaaag aggcaaatga aagcaacttt
    13201 aggaataagt agatgggaga ttcagtagaa ataaaactaa ttctccaatt tatagaatga
    13261 aattgctttt atatctaaat tgctatataa taatgataag gaggaaataa gtatgttaaa
    13321 agaagtatta accgttgcaa aagttgcgaa aaaatcttca ttatttttgg gtggtgtcgc
    13381 atttggtacc cttggtttga aaatcttagc aagtaaggaa gctaaaaaag gttattctaa
    13441 agctttggct aaggcttaca agttgaaaga cgagctagat gcatctgttt ctgttgtgaa
    13501 gcaacatgga gacgatgtct tgcaagatgc caaatatttg tacgagcaag agaaaaaaga
    13561 agagcaatta gatagcctta taggtgaata atatgtcttt taaagtgcta catagaggat
    13621 accaacatat ccgactatca tcttcttttt cacttacctt ggatattcaa gactatcttc
    13681 gttccttggc gagagatgaa aaggggattg agtctatcca gttttacatg gatcaacagc
    13741 actttactct acgcataaaa gaaggctttt ctgtattaga taatgcagaa gcctttttaa
    13801 aaagaattga taaagggaaa gtttctgagt tgatgactct tcccattcgt agagaagaga
    13861 gtgcttattc tattgtttca ggtgcagcgg ttaagcgtgt actttttcgt agttttgtgc
    13921 cgtatcctat tcgctatata tggacttgtt atcaggcttt gggttatatt agagaagcct
    13981 atcaaacact agcgcgtaag gaactaacga tggaagtctt ggactgttcg gctattttat
    14041 tgtccttgtt tatgaaccaa tccaagacag ctagcaatat catgtttatg cttgatttgg
    14101 ggaatcattt agatcagtgg tccttgaaaa aaactgcaac agatttagaa cagagtcttc
    14161 ttgcaaaaga gagcgatgta ttcctagtac agggcgatac ggttgttagt ataaagagtt
    14221 ccgatgttca aataggagat gtcttgatct tatctcaagg aaatgaaatt ctgtttgatg
    14281 ggcaagtagt ttcaggttta ggtatggtca acgaaagttc cttgacagga gagagttttc
    14341 cagttgaaaa aagagagtct gatttggttt gtgcaaatac agtattagaa actggagagt
    14401 tacgcattcg tgtaacagat aatcagatga acagccgtat tttacagctg attgagttga
    14461 tgaagaaatc tgaagaaaac aagaaaacga aacaacgcta tttcatcaag atggcggata
    14521 aagtcgtcaa atataatttc ttgggggctg ggctgactta cctattgaca ggttcttttt
    14581 ctaaggctat ttctttccta ttggtcgatt tctcctgcgc tttgaaaatc tctactcctg
    14641 tagcttattt gacagttatc aaggaagggt tgaaccgtga aatggtgatt aaggatggag
    14701 atgttctgga gaaatatctg gaagttgata ctttcttgtt tgataagaca ggaacaatca
    14761 caactagtta tcctatagtt gaaaaggtgt taccttttgg agactatagt gaggaagata
    14821 ttctcagaat cagtgcctgt cttgaggaac acatttatca tcctattgct aatgccatcg
    14881 tcaagcaagc tgagatagag ggaattgaac atgaggaaat gcatgggaaa ctccaatata
    14941 tcgcaagcaa ggggatcaaa tctcatatag atggccaacc agttcttatt gggaattatg
    15001 tcttgatgca ggatgagcag attcatatca gttcagaaca aaatgcttta attgaagagt
    15061 acaagagtca ctacaatctc ttattcttgg cttatcaaaa tgaattgatt ggaatgttct
    15121 gcattcatac tcctttgaga aaagaagcaa aaacagcctt ggataaactt aaggcacagg
    15181 ggaaaaaatt gattctggca acaggagaca ccttgattag gacagaggaa ttagtcaaag
    15241 atttgccctt tgatcaggtc tatacagact tgaaacctga tgggaaattt gagttagtag
    15301 agaaactgca gaaagcaggt cacactattt tgatggttgg agatggattg aatgactcag
    15361 cggctttaac tctatcagat atcggtgtgg tgatgaatga gagtgcagat atttctaagc
    15421 agatgagtga tatcttattg ttagataatc gtttggattt cttccaagag ttggattcgt
    15481 tatcatcatc tttgcaaaca ctcatcaaga aaaatattca agataccgtt gtcgtaaata
    15541 gtagtttgat tggctttggc ttatttaatt ggttcagtcc ttcaaatctc tctatcttac
    15601 ataatctaac aaccttgcgc attgtcttgc gtagcttgtc tattaaaaat agatagatga
    15661 gagttattaa cagcaaaaag gagttacctt tctcgaggtt aactcctttg tttatagata
    15721 aatgttgaat agtttagtaa gtcaatacaa agtatttttt cttcccacga cggataacag
    15781 tcagttcatt ttctaactta tcagcgtcac tcaagacata gtcaaggtct tggatgcggt
    15841 cgccgtttac gtagatggct ccgttttgga cgtcttcacg ggcttggcgt tttgagttaa
    15901 ctataccaga tgagacgagc agttccacga tattgttgtt ttcgtctgcc tgtacttggt
    15961 agtttggcac accacgaagt ccttgtttga gctctttgac agaaaggttt ttgatgtttc
    16021 ctgcaaagag ttgctcagtg atgttaagtg cttctttgta ggcttcttct ccgtgaacaa
    16081 gtgtaacaac ttcacgagcc aagacttttt gagccaagcg ttcgtgtggc gctgcttcaa
    16141 attgtttacg aatatcttca atctcatcaa gtgacaagaa agtaaagatt ttcaagaagc
    16201 gaatagcgtc agcgtccatc acgttcatcc agaattggta catttcgtat ggagaagtct
    16261 tttcgggatt gagccagacg gcatttcctt ctgatttacc aaatttttta ccagttgcat
    16321 ctgtgattag tggaacagtg ataacgtgac cagtcttgtc cgccttacga cgaagcaatt
    16381 cggtaccagc tgtcatattt ccccactggt cagaaccacc gatttgaaga gtgacattat
    16441 ggtcttggtt aaggacgaag aagtcgtacc cttgcatgat ttggtaagcg aactcagtgt
    16501 aagaaattcc tgtttcgatc cgttttttaa cagattcctt actcatcatg tagttgaccg
    16561 tgaagtattt tccaatgtca cggaggaagt caatgaagct gatgctgcca aaccagtcgt
    16621 agttgttgac catgacagcc ttgttttcgc cattttcaaa gtcaagaaaa cgagaaagtt
    16681 gtccttggat agacttgacc cagccatcta ctgtgtcttt tgtttggaga ctacgttcag
    16741 catctttgaa ggacggatct ccgatgagac ctgtagcacc gccaacgagc gcataaggtt
    16801 tgtgacctgc tagttgcaag cgacgacttg tcaagattgc gacaaggtgg cctaggtgaa
    16861 ggctgtcagc agttggatcg tagccagtat aataagaaac ttgaccttct tctagggctt
    16921 tacgcaaagc ttcttcatca gtcgtttgaa atatcaaacc acgctctttt agctcatcaa
    16981 aaatgtgcat gtgtcttttc tcctttttaa aattattgtt tctacctatt gtatcacaaa
    17041 cttgggcaat agcctagcag aatagggaag aaagtggcta ttttattgaa aatttccctt
    17101 ttgtggtata atagataaag tgaggaaatc catgcaaaat caattaaatg aattaaaacg
    17161 aaaaatgctg gaatttttcc agcaaaaaca aaaaaataaa aaatcagcta gacctggcaa
    17221 gaaaggttca agtaccaaaa aatctaaaac cttagataag tcagccattt tcccagctat
    17281 tttactgagt ataaaagcct tatttaactt actctttgta ctcggttttc taggaggaat
    17341 gttgggagtt gggattgctt tggggtacgg agtggcctta tttgacaagg ttcgggtgcc
    17401 tcagacagaa gaattggtga atcaggtcaa ggacatctct tctatttcag agattaccta
    17461 ttcggacggg acggtgattg cttccataga gagtgatttg ttgcgcactt ctatctcatc
    17521 tgagcaaatt tcggaaaatc tgaagaaggc tatcattgcg acagaagatg aacactttaa
    17581 agaacataag ggtgtagtac ccaaggcggt gattcgtgcg accttgggga aatttgtagg
    17641 tttgggttcc tctagtgggg gttcaacctt gacccagcaa ctaattaaac agcaggtggt
    17701 tggggatgcg ccgaccttgg ctcgtaaggc ggcagagatt gtggatgctc ttgccttgga
    17761 acgcgccatg aataaagatg agattttaac gacctatctc aatgtggctc cctttggccg
    17821 aaataataag ggacagaata ttgcaggggc tcggcaagca gctgagggaa ttttcggtgt
    17881 agatgccagt cagttgactg ttcctcaagc agcattttta gcaggacttc cacagagtcc
    17941 cattacttac tctccttatg aaaatactgg ggagttgaag agtgatgaag acctagaaat
    18001 tggcttaaga cgggctaagg cagttcttta cagtatgtat cgtacaggtg cattaagcaa
    18061 agacgagtat tctcagtaca aggattatga ccttaaacag gactttttac catcgggcac
    18121 ggttacagga atttcacgag actatttata ctttacaact ttggcagaag ctcaagaacg
    18181 tatgtatgac tatctagctc agagagacaa tgtctccgct aaggagttga aaaatgaggc
    18241 aactcagaag ttttatcgag atttggcagc caaggaaatt gaaaatggtg gttataagat
    18301 tactactacc atagatcaga aaattcattc tgccatgcaa agtgcggttg ctgattatgg
    18361 ctatctttta gacgatggaa caggtcgtgt agaagtaggg aatgtcttga tggacaacca
    18421 aacaggtgct attctaggct ttgtaggtgg tcgtaattat caagaaaatc aaaataatca
    18481 tgcctttgat accaaacgtt cgccagcttc tactaccaag cccttgctgg cctacggtat
    18541 tgctattgac cagggcttga tgggaagtga aacgattcta tctaactatc caacaaactt
    18601 tgctaatggc aatccgatta tgtatgctaa tagcaaggga acaggaatga tgaccttggg
    18661 agaagctctg aactattcat ggaatatccc tgcttactgg acctatcgta tgctccgtga
    18721 aaagggtgtt gatgtcaagg gttatatgga aaagatgggt tacgagattc ctgagtacgg
    18781 tattgagagc ttgccaatgg gtggtggtat tgaagtcaca gttgcccagc ataccaatgg
    18841 ctatcagacc ttagctaata atggagttta tcatcagaag catgtgattt caaagattga
    18901 agcagcagat ggtagagtgg tgtatgagta tcaggataaa ccggttcaag tctattcaaa
    18961 agctactgcg acgattatgc agggattgct acgagaagtt ctatcctctc gtgtgacaac
    19021 aaccttcaag tctaacctga cttctttaaa tcctactctg gctaatgcag attggattgg
    19081 gaagactggt acaaccaacc aagacgaaaa tatgtggctc atgctttcga cacctagatt
    19141 aaccctaggt ggctggattg ggcatgatga taatcattca ttgtcacgta gagcaggtta
    19201 ttctaataac tctaattaca tggctcatct ggtaaatgcg attcagcaag cttccccaag
    19261 catttggggg aacgagcgct ttgctttaga tcctagtgta gtgaaatcgg aagtcttgaa
    19321 atcaacaggt caaaaaccag ggaaggtttc tgttgaagga aaagaagtag aggtcacagg
    19381 ttcgactgtt accagctatt gggctaataa ggcaggagcg ccagcgacaa gttatcgctt
    19441 tgctattggt ggaagtgatg cggattatca gaatgcttgg tctagtattg tggggagtct
    19501 accaactcca tccagctcca gcagttcaag tagtagttct agcgatagca gtaactcaag
    19561 tactacacga ccttcttctt caagggcgag acgataattc tctaaatgaa gtggccaatc
    19621 aatggattgc cacttttttc ttttcgtgtt acaataaggg tatgaatcag tatcagaaaa
    19681 agattgttaa cggaaaaatt tattcgctcc tatccggctt aatatgggga atctgtggaa
    19741 ttttaggaga gtacttcttt actcattatc aggtgtcttc gggctggatt acctctatgc
    19801 gtttgacact ggcagggagt tttgtactca tttggtctgc aatacaatta aaatcgcaag
    19861 tgctagatat ttggcgagac aagaaaaatt acctgccctt tttagcctat gctattttgg
    19921 ggattttttc agttcagtat tttttctatc tctgtgtaga atactcaaat gctacgacag
    19981 caactatttt acagtttatt agccctgttt ttatcctctt ttacaatcgc ttggtttatc
    20041 aaaaacgagc gtcaaaaagc gctgttttct atgttttggt tgccatgctg ggtgtttgct
    20101 tgatggcgac aaagggagac ctctctcagt tatccatgac gccgctagct cttataacag
    20161 gtttgctgag tgccatgggt gttatgttta atgttatctt gccccaacct tttgctaagc
    20221 gttatggttt tgttcctacg gttgggtggg ggatgatttt ggcaggtttg tttagcaatg
    20281 tcctctcgcc ggtttatcag ctttccttta ctcttgatat ttggagtatc ttgatttgcc
    20341 tcattatcgc tttctttgga acggcttttg cttttttcat ttccatgaag gctgtgtcct
    20401 tggtttctcc tttggtggtt gccgttatca gtgccagtga acctctctct tctgctctct
    20461 tgagtgtttt gttcttagga ttagtagtgg attggtccct ccttctagct atagccttga
    20521 ttattttacc catgatattt ttgtctatag aagaagcgaa agaaagtaga taaaaagtct
    20581 tttcttaatt ctaaaagtgt gctatactag aatagtcaat aaaacacggg gagatagttg
    20641 tgaagagtgt ttttaggttg tatcggtagg ggcttgcttt taccgacagc acgttttatc
    20701 tcacatccct aaaaaatcat acctaaaaac agacaaaaag gcttcaaatt tgaggccttt
    20761 tttggtagaa taggtatcat tataacgaac caggaggcac ctatgactgc tacaaaaatg
    20821 aacgctcaag aaattatcca atttatcgcc aatgctgaaa agaaaaccag tgtcaaagta
    20881 acctttgagg ggcaactcgc aacttctgtg cctagctctg ttgtcaaact aggaaatgtc
    20941 ctattcggag actggaagga cgtggctccg cttcttgaag gtttggtaga aaatcaagat
    21001 tatgttgtcg agcaagatgc tcgtaattct gcagttcctt tgctagataa gcgtgctatc
    21061 aacgctcgta tcgagccagg tgctattatc cgtgaccagg tggaaattgg tgacaatgct
    21121 gttatcatga tgggagctgt tatcaatatc ggtgctgaaa tcggtgctgg aaccatgatt
    21181 gacatgggtg ccatccttgg tggccgtgcc atcgttggaa aaaatagcca cgttggtgca
    21241 ggtgcagttt tggcaggtgt gattgagcca gctagtgctg aaccagtccg tgtcggagac
    21301 aatgttctta tcggtgctaa tgcagtggtt atcgaaggag tccaaatcgg tagtggttca
    21361 gttgtcgctg caggagctat cgttacccaa gatgtcccag aaaacgtggt agtagcaggt
    21421 gttccagctc gtatcatcaa agaaattgat gcccaaaccc aacaaaaaac agcgctagag
    21481 gatgcgcttc gtaccttgta attgtaaaag taaaaaagag gcggaaccct ttttccagcc
    21541 tctttctgct atatcggagg acagatagat gttagatttg attcagacta gacgagcttt
    21601 acaccagatt ccagagattg gcttggagga gttcaagact caggcttatt tgctggatgt
    21661 gattgagaaa ttgactacgg gcaaggattt tgttcaaatt cgtacttggc ggacagggat
    21721 tttggtctac ctgcagggaa gtcagccgga gcgaaccatt ggttggcgga cagatattga
    21781 tggcctgcct atcgtcgaac aaacaggtct accttttgct tctcaacacc aaggtcgcat
    21841 gcatgcctgt ggccatgatt ttcatatgac cattgcctta ggctgtcttg agcgcgccct
    21901 tgaggagcaa cccaagaata atctgctctt tctatttcag cctgctgaag aaaatgaagc
    21961 tggtggtatg ctcatgtatg aggatggtgc ttttggagat tggttgccaa accaattcta
    22021 tggtctccat gttcgtccag atttgaaagt cggccagatt gcgactaata ctcatacact
    22081 ctttgcagga acttgcgagg tgaagatccg tttcaaaggc aaaggtggcc acgcagcttt
    22141 tcctcatgaa gccaatgacg ccttggtggc ggctagttac tttgtgaccc aagtgcagtc
    22201 agttgttagc cgcaatgtca acccaatcga gggagcagtg gtgaccttcg gcgtttttca
    22261 agctggaacc accaacaatg tcatcacaga cacagctttt ttacatggaa ctattcgtgc
    22321 cttgacacag gacatgagtc tcttggtgca aaagagggta aaaacagttg cagaaggggt
    22381 tgcagctgcc tttgatatgg aagtagaagt ggaactcaaa caaggtggat acctacctgt
    22441 tgagaacaat ccagccttgg cgcgtgaact gatggacttc tttgacgaaa aagacggaat
    22501 cgagttgatt gatatcgaac ctgctatgac aggcgaggac tttggttatc tcctttcaaa
    22561 ggttgatggc gtgatgtttt ggctaggtat cgatagtccc tacgctcttc atcaccctca
    22621 aatgagtcct aaggaagaag tcttagctat tggggtggct gcggtgtcta gtttcctgaa
    22681 aaagaaggca gcagaataga gggcttgtct atgaaatcgg aattacgcaa gcaagtcttg
    22741 catgaaatga aggctatatc tcaagaacaa aaacagttta tagatcaaac tttaaccgag
    22801 cggcttttac accatccctt ctatcaagaa gccaaggtca tcgcaaccta tctctctttt
    22861 cctcatgagt ttcaaacgca ggaactgatt gagcaggcgc tgaaggacgg caagaaggtt
    22921 ttaataccca aaacttatcc caaggggcgc atggactttg tggtctatga tccgcaacag
    22981 ttggtaaaaa cttcctttgg attactggag ccacagggag atttggaagt ggtggatgca
    23041 tctcagattg atttgattca tgttcctggc ctggctttta cgacgaaagg atatcggata
    23101 ggatacggtg gaggctatta tgaccgctat ctggaacatt tttctggtcg tactttgagt
    23161 acgatatatc cttgtcaaat tcaggacttt atacctgaaa accatgatat tcctgttcag
    23221 gaggtattaa ttgatgaagg aaatctttga tagacgttac cctgtgacga gtttcttcct
    23281 cttagtgacg gccttggtat ttttactaat gttggtcact gcaggcggaa actttgacag
    23341 ggcagataca ttatttcgat ttggagccat gtatgggcca gctattcgcc tctttcccga
    23401 gcaggtttgg cgtctcttgt ctgccatttt tgttcatatt gggtgggaac atttcattgt
    23461 taatatgctt tcactttatt atcttggaag gcaggtagag gagattttcg gttctaagca
    23521 gtttttcttt ctctatctct tatcaggaat gatgggcaat ctctttgttt ttgtatttag
    23581 tcctaaatcc ttagcagcag gcgcctctac ctctctttat gggctatttg ccgcgattat
    23641 cattcttcgc tatgcaacgc gtaatcctta tattcagcag ttgggccaat cctatctgac
    23701 actttttgtg gttaacatca ttggaagtgt tctgattcca ggaatcagcc tagcaggcca
    23761 tatcggtggt gcagttggtg gcgcatttct agcagttatc tttccagtta gaggagaaaa
    23821 acggatgtat aacaccagcc agagattagg agcggtagtc ttgttcgtag gactcgccat
    23881 tttgcttttc tacaagggaa tgggaatgtg acgaagttga aagaaaattt gtgtaatgaa
    23941 aaaagataag gcattttttg aaccttatct ttttatttta ttccttctca gtcaattctt
    24001 ttccaagttg gatgaggtaa ttcttcaaat catctttgac ttgtgggtgt ttgagggcgt
    24061 agtcgatgga tgttttcatg aagccaaact tgtctccgac atcgtaacga gcccctttga
    24121 actcacgagc aaatacacgt tgtgttttat tgagggtgtc gattgcatct gtcagctgaa
    24181 tttcatttcc tgcacctgga gcttgctttt cgagaatctc aaaaatttca ggcgtgagga
    24241 ggtagcgtcc gataatagca aggtcgctag gagcgtcctc tggagctggt ttttcaacaa
    24301 aggtttcaac actgtaaaga ccatcttttc cttcgccttg cggagcaata accccgtaag
    24361 cagatacttc gtcatgaggg actggcatga cagcgatagt agacgcgtgg gtacgctcgt
    24421 agtcatccat gagttgtttg gtaagtggaa cagccttttc gtctgtgata tccatcaagt
    24481 catcaccaag cataacgaca aaaggttcat ttccgacgaa agccttggct tgcaaaacag
    24541 catctccgag accgcgtgga tgagtttggc ggataaaatg cagacgcatg ccagttgttt
    24601 catcaactag cttcaaaaga tctgttttcc ctttttcttt gaggttatat tccaattcga
    24661 agtttgaatc gaagtggtcc tcaatagaac gttttgattt accagtgaca actagaatat
    24721 cttcaatacc tgatttgaga gcctcttcca cgataaactg gatagttggt ttgtctacga
    24781 ttggcaacat ttctttggca agggccttgg ttgctggtaa aaatcgagtt cctagtccag
    24841 cagcagggat aactgccttt ctaacttttg atgtcataaa aatcctttct atagagggtt
    24901 aagaccactc attttctgct ttaaattcat tgttcatgat gtcataaatg gcatctttga
    24961 tattggttcc gtgataaata acttggtaaa tagcctgtgt aatgggcata tagactccaa
    25021 gttcttgggc tagttcatag gctgctcgag tcgttgaaat tccttcgatt accatgccca
    25081 tattagcttc tatatcagct agggattctc ctcgtccgag agcatctcca gctctccagt
    25141 tacgagagtg gatggaagtt cccgttacga tcaaatctcc cacaccagat aagccgctat
    25201 aggtcaatgg actggccccg agtgctaccc ctaggcgggt gatttctgct aaacctcgag
    25261 cgatgatggc tgccttagca ttatcaccaa atccaagacc atgtaaagct ccagcaccga
    25321 cagcaataat atttttaaga gcaccagcag tttcaacccc gataacatcc gtattggtat
    25381 aaagtcggaa gtagtgatta ctaaatagct tctgaacgta ttgagctgtt tgtaaatctt
    25441 tagaagcagc agttattaaa gttaggtcac gcacaatggt ctcttctgca tgactaggcc
    25501 ctgaaacaac gacgatatca ctacggagat gttcaggaat ttcttcttca agaatggttg
    25561 ataattgttt atggctatca ggttctaatc cctttgatgc gtgcatgatg ataaccttat
    25621 ggtccaaggt ttgtgcaact tgctgggcaa caagtcgtgt cacttttgtt gggacaacaa
    25681 acaaaatcgc atccacatct ttcaatgttt ctgctaagtc ggtgtaggca atgatatttt
    25741 cgtctagaac gacatcttta aagtagtgct tattagtatg gtgtgtatta atttcattga
    25801 tttgctcggg aagatttccc caaatacgta cctcgtgtcc attgtcattt aagacttgtg
    25861 aaagggcggt tccccaagaa ccaggcccca agacggcgac ggtttgtttt tccatgattt
    25921 cctccttgat agagttcttt ctcttattct atcatattct aggggatttt gcacttgcat
    25981 tactggggta tggtggaaaa atacacaaaa accgagacga agggataaac tcttagtctt
    26041 tagttaaagt gagaagaaat ctgatagata gtttgattca gtatatgctt ataatagagg
    26101 taaacaactc aggaggttct tgtaggttgc gagagtttgg cgaaaaaatt aaaagattac
    26161 gtttggctaa aaaaatcagt cgttcagaat tttgtggtga tgagtctgaa ttaagtatcc
    26221 gtcaattaat tagaattgaa aatggagaat ccagaccaac actaacaaag ttaaaatata
    26281 tcgctgaacg tttgggggtt gaggattaca agttgatgcc aagttatata gagttggata
    26341 aggaatacct agaattgaag tatttcttga tgaggactcc tacatacgaa gatgaaacta
    26401 tcgcccaaaa gaaagagagt gttttgataa gatttttgaa gagtattatg ataggctacc
    26461 tgaggaagaa agatttatca tcccaaatta ttcatatctg gcactaacga actacacagt
    26521 tcaaaaatta ccagaaaagc tagttgaaat actgtccttc tggtgatttt gcttaatttc
    26581 tgatgttaga gacataattg aggagatttg tcttgatttt ttcctaaaaa agggcagact
    26641 cctcccttgg tttgatgctc gatttttaaa ataaggtgac ttattctgtt aggcatgttt
    26701 tcccctgccc tctagctcta tatggaacag ggagtatcag atttatctgg cagattgtat
    26761 caaataaggc ttgaaactgt tttgaatagg catatagact tgagaatttg agaatatgtc
    26821 gtctagcagt agagacatat tttccggcaa tatgaaggaa gagacgtcga aaacgcttga
    26881 tagtcaaggc ctttacttca tcaccagcta gctgctttaa aaagaggtag agattataag
    26941 ccagacagcc catcatcata cgtacttcat tcttaatcat ggtcgaacta tctgtcttat
    27001 ccccaaagaa tcctgctttc ctttctttga taaagttttc agcattccct cgttcacgat
    27061 agaattgaaa cagctcctca ctatctacac tgcttaaatt ggtcaaatga acttttt
//
LOCUS       DAVDWM010000026        26282 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVDWM010000026 DAVDWM010000000
VERSION     DAVDWM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.026"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            127..624
                     /locus_tag="VUW49_001281"
     CDS             127..624
                     /locus_tag="VUW49_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7981200.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            624..2279
                     /gene="dnaX"
                     /locus_tag="VUW49_001282"
     CDS             624..2279
                     /gene="dnaX"
                     /locus_tag="VUW49_001282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7981201.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLPALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLAGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2307..2501
                     /locus_tag="VUW49_001283"
     CDS             2307..2501
                     /locus_tag="VUW49_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET7981202.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2651..3421
                     /gene="sufC"
                     /locus_tag="VUW49_001284"
     CDS             2651..3421
                     /gene="sufC"
                     /locus_tag="VUW49_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7981203.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3449..4711
                     /gene="sufD"
                     /locus_tag="VUW49_001285"
     CDS             3449..4711
                     /gene="sufD"
                     /locus_tag="VUW49_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7981204.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4722..5948
                     /locus_tag="VUW49_001286"
     CDS             4722..5948
                     /locus_tag="VUW49_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7981205.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPAIARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5935..6375
                     /locus_tag="VUW49_001287"
     CDS             5935..6375
                     /locus_tag="VUW49_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET7981206.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6429..7841
                     /gene="sufB"
                     /locus_tag="VUW49_001288"
     CDS             6429..7841
                     /gene="sufB"
                     /locus_tag="VUW49_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7981207.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVICELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8040..9281)
                     /locus_tag="VUW49_001289"
     CDS             complement(8040..9281)
                     /locus_tag="VUW49_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET7981208.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKIWWNQFVRFVNEKL"
     gene            9384..10493
                     /locus_tag="VUW49_001290"
     CDS             9384..10493
                     /locus_tag="VUW49_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7981209.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11139..11486
                     /gene="ssrA"
                     /locus_tag="VUW49_001291"
     tmRNA           11139..11486
                     /gene="ssrA"
                     /locus_tag="VUW49_001291"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11656..11743)
                     /locus_tag="VUW49_001292"
     tRNA            complement(11656..11743)
                     /locus_tag="VUW49_001292"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11707..11709),aa:Ser,seq:gga)
     gene            11905..12645
                     /locus_tag="VUW49_001293"
     CDS             11905..12645
                     /locus_tag="VUW49_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7981210.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12642..13553
                     /gene="pfkB"
                     /locus_tag="VUW49_001294"
     CDS             12642..13553
                     /gene="pfkB"
                     /locus_tag="VUW49_001294"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET7981211.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSDGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13550..15502
                     /locus_tag="VUW49_001295"
     CDS             13550..15502
                     /locus_tag="VUW49_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7981212.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15666..17891
                     /locus_tag="VUW49_001296"
     CDS             15666..17891
                     /locus_tag="VUW49_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET7981213.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17936..18370)
                     /locus_tag="VUW49_001297"
     CDS             complement(17936..18370)
                     /locus_tag="VUW49_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981214.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNTKKEPTTSQTTTIVKEIQKESPQENTSPNKENKTSQEKTQQEETPKSSVKE
                     EKKEEQKTSTQDSSTPAPTPSKPATENEKQSNTPTSENKTNQ"
     gene            18508..19650
                     /locus_tag="VUW49_001298"
     CDS             18508..19650
                     /locus_tag="VUW49_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7981215.1"
                     /translation="MIYFDNSATTKPYPEALETYVQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19659..20873
                     /gene="thiI"
                     /locus_tag="VUW49_001299"
     CDS             19659..20873
                     /gene="thiI"
                     /locus_tag="VUW49_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET7981216.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYAQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21008..21821
                     /locus_tag="VUW49_001300"
                     /pseudo
     CDS             21008..21821
                     /locus_tag="VUW49_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            21843..22582
                     /locus_tag="VUW49_001301"
                     /pseudo
     CDS             21843..22582
                     /locus_tag="VUW49_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22595..23761
                     /locus_tag="VUW49_001302"
     CDS             22595..23761
                     /locus_tag="VUW49_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET7981217.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYHGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            24269..25762
                     /locus_tag="VUW49_001303"
     CDS             24269..25762
                     /locus_tag="VUW49_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET7981218.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25775..>26282
                     /locus_tag="VUW49_001304"
     CDS             25775..>26282
                     /locus_tag="VUW49_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7981219.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLL"
BASE COUNT         7851 a         4836 c         5763 g         7832 t
ORIGIN      
        1 aaaaataggc tccataatat ctataaggga tttacccact acaaatatta tagagcccaa
       61 aatcctttgt ttactaaaca agggattttt cttttgtctc tgctcctttt ttgatataat
      121 agttctatgt taaaatcaga aaaacaatca cgttatcaaa tgttaaatga agaattgtcc
      181 ttcctattgg aaggcgaaac caatgttttg gctaatcttt ccaacgccag tgctctcata
      241 aaatcacgtt ttcctaatac cgtatttgca ggcttttatt tgttcgatgg aaaggaattg
      301 gttttaggcc ccttccaagg aggtgtttcc tgcatccgta ttgcactagg caagggtgtt
      361 tgtggtgagg cagctcactt tcaggaaact gttattgttg gagatgtgac gacctatctc
      421 aactatattt cttgtgatag tctagctaaa agtgaaattg tggtgccgat gatgaagaat
      481 ggtcagttac ttggagttct ggatctggat tcttcagaga ttgaggatta cgatgctatg
      541 gatcgagatt atttggaaca atttgtcgct attttgcttg aaaagacaac atgggacttt
      601 acgatgtttg aggaaaaatc ttaatgtatc aagcacttta tcgaaaatat agaagtcaaa
      661 acttctccca gttagttggt caagaagttg tggctaagac tcttaaacaa gcggtggagc
      721 aagagaaaat aagtcacgct tatctttttt ctggtcctcg tggaacggga aaaaccagtg
      781 ttgctaaaat ctttgccaag gctatgaact gtcccaatca agtgggtggc gaaccttgca
      841 ataactgcta tatttgtcaa gcagtgacgg acggtagttt agaagatgtc attgaaatgg
      901 atgcagcttc taataatggg gtagatgaaa ttcgcgaaat tcgtgataaa tctacctatg
      961 cgcctagcct tgctcgttat aaggtttata tcatagatga ggttcacatg ctgtctacag
     1021 gggcttttaa tgccctctta aagacgctgg aagaaccaac acagaatgta gtctttattt
     1081 tggccactac tgaattgcac aagattcctg ctactattct atcccgtgtg caacgttttg
     1141 agtttaaatc aattaagaca caggatatta aggaacatat tcactatatc ttagaaaaag
     1201 aaaatatcag ttctgaacca gaggctgtgg aaatcattgc cagacgggcg gaaggtggaa
     1261 tgcgggacgc cttgtctatt ttggatcaag ccctgagttt gacacaggga aatgagctga
     1321 cgactgctat ctctgaagaa attactggca ccattagcct accagccttg gatgattatg
     1381 tggcggcctt gtctcaacag gatgttccca aagctttgtc ttgcttgaat cttctttttg
     1441 acaatggtaa gagcatgact cgttttgtga ccgatctttt gcactattta agagacttgt
     1501 taattgttca aacaggggga gaaaatactc atcatagttc agtctttgta gaaaatttgg
     1561 cacttcctca aaaaaatctg tttgaaatga ttcgcttagc aacagtgagt ttagcagata
     1621 ttaagtctag tttgcagccc aagatttatg ctgaaatgat gaccgtccgt ttggcggaaa
     1681 tcaagtccga accagctcta tcaggagcgg ttgaaaatga aattgctacg ctgagacagg
     1741 aagttgcccg tctcaaacaa gagctttcta atgtaggtgc ggttcctaaa caagttgcac
     1801 cagctcctag tcgaccagct acgggcaaaa cagtctatcg tgtcgatcgc aataaagtgc
     1861 aatctatctt acaagaggcc gtcgaaaatc ctgatttagc acgtcaaaat ttaattcgtt
     1921 tgcagaatgc ctggggagag gtaattgaaa gtctaggtgg gccggacaag gctctgctag
     1981 ctggttctca accggttgct gccaatgaac accatgctat tcttgctttt gagtctaact
     2041 tcaatgctgg tcaaactatg aaacgagaca atctcaatac catgtttggt aatatcctca
     2101 gtcaggcggc aggtttttca cctgagattt tagctatttc catggaggaa tggaaagaag
     2161 ttcgcgcagc cttttcagcc aaagccaaat cttctcaaac tgaaaaagaa gtagaagaaa
     2221 gcctgattcc agaaggattt gaatttttgg ctgataaagt gaaggtagag gaagactaaa
     2281 gaaagatttc atgatacaat aagtttatga ataaacaaca atttattatt atggcgctat
     2341 ttacagctgc tgagacctat tttttcaatg aagcctggat gactggccgc tatattatgg
     2401 cagccttttg ggcaatttta ctctttagaa atttccgagt cagttatgtg atgggtaaaa
     2461 tcgttgatgt catcgatcag cattttaata ggaaagacta gccctcagct tccagacaaa
     2521 atcaaagcct tttaggcttt tttttgttat actagaaaag tatatttata gaatttttgc
     2581 tctatttctg gggaaatcag acgtttttct agtaagtact gtaaaagttt tgaaaaagaa
     2641 aggaactatc atgtcagtaa tagagatcaa agatcttcac gttgagattg aaggaaaaga
     2701 aattttaaaa ggggttaacc tgaccctgaa aacaggagaa attgccgcta tcatgggacc
     2761 aaatggtaca ggtaaatcga ctctttctgc cgctatcatg ggaaatccaa actatgaagt
     2821 aactaaaggt gaagttttgt ttgatggcgt aaacatcctt gagttggaag tggatgagcg
     2881 tgcgcgtatg ggacttttcc ttgctatgca atacccatca gaaatccctg gaattaccaa
     2941 tgctgaattt cttcgtgccg ctatgaatgc gggtaaagaa gatgatgaga agatttcagt
     3001 tcgtgagttt attactaagc tagatgaaaa aatggaattg cttaacatga aagaagaaat
     3061 ggcagagcgt tacctcaacg aaggcttctc tggtggtgag aaaaaacgca atgaaattct
     3121 tcaacttttg atgttggagc caacatttgc tcttttggac gagattgact caggtcttga
     3181 tattgacgct cttaaagttg tgtctaaagg tgtcaatgcc atgcgtggtg aaggttttgg
     3241 tgctatgatc atcactcact accaacgtct tttgaactat atcacacctg atgtggtaca
     3301 cgtgatgatg gaaggtcgtg ttgtcctttc tggtggtcca gaattggctg cgcgtttgga
     3361 acgtgaagga tacgcaaaat tagctgaaga acttggctac gactacaagg aagaattgta
     3421 attccctcgt atcttttagg agaagtaaat gactagagaa aatattaaac ttttttcaga
     3481 aatgcacgct gaaccaagct ggttggctga tctccgtcaa aaagcttttg acaagattga
     3541 gactttggaa ttaccagtta ttgagcgtgt caaattccac cgttggaatc tgggtgatgg
     3601 aacgattaca gaaaatgagc catcagcaaa tgttccagat ttcacagctt tagatcatca
     3661 cttgaagttg gtgcaagtag gaactcaaac tgttttcgaa caaactccag ttgagttagc
     3721 tgaacagggt gttgtcttca cagactttca ctcagcttta gaagaaattc cagagctgat
     3781 cgaagaattc ttcatgtcat ctgttaagta tgatgatgac aagttggcgg cttaccacac
     3841 agcttacttt aacagtggtg ctgtactcta tattccagat aacgtagaaa tcacagagcc
     3901 aattgaagga attttctacc aagatagcga tagcaatgtg ccgtttaaca agcatattat
     3961 gattatcgct ggtaaaaatt ctaagattag ttatctggag cgtttagagt cacgcggtga
     4021 aggaagtgac aaagcaactg ccaatatcac agtggaagtg attgcacgtt ctggtgcgca
     4081 agtcaagttt gctgctatcg accgtctagg tgaaaacgtc actgcctaca ttagccgtcg
     4141 tggtaaatta ggcaacgatg caagtattga ctgggctatc ggtgtcatga acgaaggaaa
     4201 tgtcgttgct gattttgata gtgacttgat tggtaatggt agccatgctg acctcaaggt
     4261 tgtagctctt tcaagtggtc gtcaggtaca agggattgat actcgtgtaa ctaactatgg
     4321 ctgcaactca atcggaaaca ttctacaaca tggggttatc cttgaaaaag caactttgac
     4381 tttcaatggt atcggccaca tcatcaaggg tgctaaggga gcagatgcgc aacaagagag
     4441 ccgtgttctc atgctttcag accaagcgcg ttcagatgct aacccaattc ttttgattga
     4501 tgaaaatgac gtaactgcag gccatgcagc ctctattggt caggtagatc cagaagatat
     4561 gtactacctc atgagtcgtg gcttggataa ggcaactgca gagcgtttgg ttgttcgtgg
     4621 tttccttgga tctgttatcg tggagattcc agtcaaggaa gttcgtgatg aaatgattgc
     4681 aactatcgaa gagaaattgt caaaacgcta aggggcagcc tatgttagat gtagaagcga
     4741 ttcgcaagga ttttccaatt ttagatcaga ttgtaaatga tgaacctctg gtctatctgg
     4801 acaatgctgc gacgacacaa aaaccactag tagttctgaa agctattaac agctactatg
     4861 agcaggacaa tgccaatgtt caccgtggtg tccatacctt agcggaacga gcgacagctt
     4921 cttatgaagc tgctcgtgaa accattcgta agtttattaa tgcaggctct acaaaggaag
     4981 ttctctttac cagaggaacg acaaccagcc ttaactgggt ggcacgcttt gctgaggaaa
     5041 ttctcactga gggagaccag gtcttgattt cagtaatgga acaccattct aatatcattc
     5101 catggcagga agcttgtcga aagactggag cagagcttgt ctatgtctat cttaaagacg
     5161 gtgccttgga tatggaggat ttgcgagcta aattgactga taaggttaaa tttgtttccc
     5221 tagctcatgc ctccaatgtt cttggtgtgg tcaatccgat caaggaaatc actcaattag
     5281 cccaccaagt tggggcaatc atggtagtgg atggtgctca atctacacct catatgaaga
     5341 ttgatgtcca ggacttggat ctggactttt tcgccttttc gggtcacaag atggctggtc
     5401 cgactggtat cggtgtcctt tacggcaaag aaaagtatct tgagcaaatg tctccagtag
     5461 aatttggcgg cgagatgatt gattttgtct acgagcaatc tgctagttgg aaggaattgc
     5521 cttggaaatt tgaggctgga acgccaaata tggcaggagc tattggactt gcgactgcag
     5581 ttgattatct ggaaaagatt ggtatggatg ccattgaagc tcatgaacag gaattgattg
     5641 cgtacgtcta tccaaaactg caggcaattg agggattgac catttacggt tctcaggatt
     5701 tggctcaacg ttcgggtgtt attgccttta acctaggtga tctccatcct cacgatcttg
     5761 cgacggctct ggattatgaa ggagtggctg ttcgtgctgg tcaccattgt gcgcaaccct
     5821 tgcttcagta tttggaagtc ccagcaatag ctcgtgcaag tttttatatc tacaatacca
     5881 aggcagattg cgacaaacta gtcgatgccc tacaaaagac aaaggagttt ttcaatggca
     5941 ctttctaaac tagatagcct ttatatggca gtggtagcag accattcgaa aaatccacat
     6001 caccaaggga agttagaaga cgctgagcaa atcagtctca acaatccgac ttgtggggat
     6061 gtcatcaacc tctctgtcaa gtttgatgca gaggaccgtt tggaagatat tgcttttcta
     6121 aattcaggat gcacgatttc aactgcttct gctagtatga tgacagatgc cgttttagga
     6181 aaaaccaaac aagaaatttt agaactggcg actatttttt ctgaaatggt tcaagggcaa
     6241 aaagatgagc gtcaagacca acttggagac gcggcattct tgtcaggtgt tgccaaattc
     6301 cctcaaagaa tcaagtgtgc aaccctagct tggaatgccc ttaagaaaac aattgaaaat
     6361 caagaaaaac agtaagacaa gtttcttttg tcttatgaat tattagaaat gaagaaagaa
     6421 aggatattat ggctgaagaa agagtagaac caaaaccaat tgaccttggt gaatataaat
     6481 ttggtttcca tgacgatgta gagcctgtct tatcgacagg aaaaggactc aacgaaggtg
     6541 ttatttgtga attatctgct gctaagggtg agcctgagtg gatgttggag ttccgtttga
     6601 agtcttatga aaccttcaaa aaaatgccca tgcaaacttg gggagcagac ttgtcagaga
     6661 ttgactttga tgacttaatc tactaccaaa aaccatctga caaaccagcc cgttcttggg
     6721 atgatgtacc tgaaaagatt aaagaaacct ttgaacgtat cgggattcca gaagctgaac
     6781 gtgcttattt agcaggggct tctgcccagt acgagtcgga agtggtttac cacaacatga
     6841 aggaagagtt ccaaaaatta ggtattatct ttacagatac agattccgca ctcaaggaat
     6901 acccagactt atttaaacaa tactttgcga agttggtacc gccgacagat aacaagttgg
     6961 cagccctcaa ctcagcagta tggtcgggtg gaacttttat ctacgtgcca aaaggtgtca
     7021 aggtagatat tccacttcaa acttatttcc gtatcaataa cgaaaatata ggtcagttcg
     7081 aacgtacctt gattatcgtt gatgagggag caagcgtcca ctacgtagaa ggatgtacag
     7141 caccaacata ttcaagcaat agcttacacg ctgccattgt agaaattttt gctttggacg
     7201 gagcttatat gcgttataca actatccaaa actggtctga taacgtctat aacttggtaa
     7261 caaagcgtgc taaggctcaa aaggatgcca ctgttgagtg gattgatgga aacttgggtg
     7321 ccaaaacgac tatgaaatat ccatctgttt accttgatgg agaaggagcg cgtggtacca
     7381 tgctctctat cgcctttgct aatgcagggc aacaccaaga cacgggtgct aagatgattc
     7441 acaatgctcc acataccagc tcgtctattg tgtctaaatc catcgctaaa ggtggaggaa
     7501 aggttgacta ccgtggacaa gtcaccttta acaagaactc taagaaatct gtttcccaca
     7561 ttgaatgtga taccattatc atggatgact tgtcagcatc agatactatt ccatttaatg
     7621 aaattcacaa ttctcaagtg gctttggaac acgaagccaa agtatctaag atttcagaag
     7681 agcaattgta ttatctcatg agccgtggat tgtcagaatc tgaggcaact gaaatgattg
     7741 tcatgggatt tgtagaaccc tttacaaaag aacttccaat ggaatacgca gttgagctga
     7801 accgcttgat tagctatgaa atggagggat cagttggata aaatttgatt ttatactctt
     7861 cgaaaatctc ttcaaaccac gtcagcatcg ccttaccgta tgtatggtta ctgacttcgt
     7921 cagtttcatc tacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt
     7981 gttctttgat tttgaagatg aacttgtatc aaaaaatcgc ggtttaaaat cgcgattttt
     8041 tataatttct cgttaacaaa gcggacaaac tgattccacc aaatttttaa gaagaaggct
     8101 ttttcaattt tcttgtctgc taccatttcg aaactagggc gctctgtggt gatgtaacct
     8161 tgaccaatca agtccttgtc ttcataagtc aaatggccaa ccactgttcc agcttcaagt
     8221 ggtgctggga ttgctttgga atcaggtgtg aattgaacag attgggaaga ttgattccca
     8281 acacgttcta ttagatagat atcctctgga gccactgcag ttactgtatc ttcttttcca
     8341 tcttgtacag gggctttgct atcttgatag gcatcgcctt gttgaacgat tttgcgaagt
     8401 gtaaatgtag aagaaatata atccattagg gaagatgtag ctgtaaatcg agcgtaagga
     8461 ttattgtctt gatgatctgc atttaaaaca actgtgatga ctctcatgcc tttttcgaca
     8521 gtagtaccaa caaaagactc tccagcctta tctgttgttc ctgtttttag cccatcaaaa
     8581 ccaccacggt aagcaggcat accttctaac atgtagttgg ttgaagtgat tgtcatccca
     8641 gcaaaagtag aagaaggttt tttggtgatt tctaagactt gtgggtattt tttgatgagg
     8701 ttgcgagcaa cgatagcgac atcataagca ctaagcttat tttcctcatc ttttttagaa
     8761 cctgggtaaa tgttatcccc tagagtttca ttgttaagac ctgtcgtatt gacaacagtg
     8821 gcatcctgaa ttccccattc caagagtttt gcccgcatca tatcgacgaa atctttttct
     8881 gagccagcaa ttttctcagc tagggcaata gcggcgctgt tggcactaga taccagagtt
     8941 gcttcaagca actcttcgac agtataatta cgggcctcca taggaatatt actggcttca
     9001 gaatttgtcg tcaattgata aggataatca gaaatatcta caggagtgga gagggtaata
     9061 cttccgtttt ccaaagcttc atagaccaga taaacagtaa tcaattttgt tatggaagca
     9121 atttcgacag gttgcgttgc atccttctca tagagaattt taccagtatt tgcctcaaca
     9181 gcaatcgcat gtttagcggc aatggtaaaa tcttgagcaa cagcagtaga agcaccccct
     9241 aaaagagaga cagttaacaa agttaaaaat atttttttca tagtagtctt attctatcat
     9301 aaagaaaaaa aatattcttg ctttaataat tcatctgtta agctttttga aaatatggta
     9361 aaataaagtg agggaggtaa ctcatgtttc gtagaaataa attatttttt tggaccacag
     9421 aaattttact cttaaccatc atcttttacc tatggagaca gatggggtct ttgattaacc
     9481 cttttgttag cgtgcttaat acaattatga ttccattttt attagggggc tttctttatt
     9541 atttgacaaa ccctattgtt actttcttaa ataaagtctg taaactcaat cgtttgcttg
     9601 gtattttaat taccttgtgt actttggtct ggggaatggt cataggtgtt gtctatctct
     9661 tacctatttt gattaatcag ttatctagtt tgattatatc tagtcaaact atttatagtc
     9721 gagtacaaga cttaatcata gacttatcta attatcctgc gctccagaat ttggatgtag
     9781 aagctacaat tcagcagtta aacttatcct atgttgatat tcttcaaaat atcctaaata
     9841 gcgtatcaaa tagtgtgggg agcgtcttgt cagctcttat cagtactgtt ttgattttga
     9901 ttatgactcc agtttttttg gtttatttct tattagatgg acataaattc ttgcccatgc
     9961 ttgaaagaac gattctaaag agggatcgct tgcatattgc aggcttatta aagaatttaa
    10021 atgcgacgat tgctcgctat attagtggag tttcgattga cgcaatcatt ataggttgtt
    10081 tagcttatat tggctatagt attattggtt taaaatatgc tttagttttt gccatttttt
    10141 ctggtgtagc caatttaatt ccttatgtgg ggccaagtat tggtttgatt cctatgatca
    10201 tcgcaaatat attcactgat cctcatagac tgctgattgc agtgatttat atgcttgttg
    10261 ttcagcaggt agatggcaat atcttatatc ctcgaatcgt aggaagtgtt atgaaggttc
    10321 atccaatcac gattttagtt ttacttttgt tgtcaagcaa tatctatggt gtagttggaa
    10381 tgattgtcgc agtgccaacc tattctatct tgaaagaaat ttctaagttc ttatcccgtt
    10441 tgtatgaaaa tcataaaata atgaaagaac gagaaagaga attagctaag taaaagtcag
    10501 gagaaccctg atttttcttt actggaagtg gcctttagat tagaagactg aaaataagtt
    10561 aaagtcttaa actaattttc acagctaaga atagtagaag ttaatctgat aaaaatcgaa
    10621 aaaaccagtg gaattctgtg tcagggtaag ttccactggt tttcatagtc tattaaagtt
    10681 cgaatgaaac ctatttatag tatattgaaa ctagaatagt acacctctaa ttctaaaaca
    10741 ttgttagaaa tcgatttgac tgtcctaatc tattcgtcct attcttattt cattttacta
    10801 tattttggtg caataagtga aaagtagtcc gaataatata aggattgatt ttatagtttt
    10861 taaactcaaa tgaattaaaa taaagagagt acgaaaattc tcatctgaaa gtattttaga
    10921 ataattctct tcgtgaattt cttcaaaaca gatagcttca tcttaggtat gtgttaattg
    10981 gatttcgtaa gctctattta caatcgtaaa gttatgcact gagaaagttg tagttagtct
    11041 tccagtttgc taaatttctc gaatagtatc ttttttaggt atgtgatttc tttttgcatt
    11101 tttgagttag ataaggtata atagttttat tgtcttttgg ggtcgttacg gattcgacag
    11161 gcattatgag gcatattttg cgactcgtgt ggcgacgtaa acgctcagtt aaatataact
    11221 gcaaaaaata acacttctta cgctctagct gcctaaaaac cagcaggcgt gacccgattt
    11281 ggattgctcg tgttcaatga caggtcttat tattagcgag atacgattaa gccttgtcta
    11341 gcggcttgat aagagattga tagactcgca gtttctagac ttgagttatg tgtcgagggg
    11401 ctgttaaaat aatacataac ctatggttgt agacaaatat gttggcaggt gtttggacgt
    11461 gggttcgact cccaccggct ccattattcc tttgcattct tttgcattcc ttggtaaaac
    11521 gttgttaaat caacgttttt tatttttata tttggtattc ctttgcattc ttttgctaaa
    11581 aagggagtca caaacagacc ctattttaaa aaaggataca acatttgtcg catcctaaaa
    11641 ataatctttt ttcgacggaa gacatgggat tcgaacccac gcacgctatt acacgcctac
    11701 cgcgtttcca acacggcctc ttaagcctct tgagtaatct tccaatactt actcaaatag
    11761 tctaccataa aggctcttat cttgcaataa aaattctaga aataagaaaa atgatagatt
    11821 ttgaaagaaa atgataaaaa atgcttgact tcgaaataaa gtatggtaga atgaatagtg
    11881 taaacgataa caggaggtga ttcagtgtta aaaacagagc ggaagcaact gattttagag
    11941 gagttaaatc aacatcatgt agtttctcta gaaaaattag ttagtttgct agaaacgtca
    12001 gaatcaacgg ttcgaagaga cttggatgag ttggaagcgg aaaacaagct tcgtcgtgtg
    12061 catggtggag cagaactccc ccactcctta caggaagaag aaaccattca agaaaaatct
    12121 gtcaaaaacc ttcaagaaaa gaagttgctg gctcagaaag cagcctctct cattaaagaa
    12181 aaagatgtca tctttatcga tgctggaaca acaactgctt ttttgattca tgaattggtc
    12241 aataagaata ttacagttgt gaccaactcc attcaccatg ccgctcagtt ggttgaaaag
    12301 cagattccaa ctgtcatggt tggaggaaac gtcaagacgg cgacagatgc tagtatcggt
    12361 ggcgttgctc ttaaccagat taaccaattg cactttgacc gtgcctttat cggaatgaat
    12421 ggtgttgacg atggctatta tacgactcct gatatggagg agggagctgt gaaaagagct
    12481 attttggaga atgccaagca gacctacgtc ttggtggatt cgtcaaaaat tggacaaact
    12541 tgctttgcca aggtagcacc actcaaacgc gctatcgtta tcactagtca agggcatgag
    12601 ctcttgcagg ttattaagga gaaaacggag gtaatagaag tatgatttat acagtcacac
    12661 tcaatccatc cattgactat atcgttcgtt tggaccaagt caaagttggt agtgtaaatc
    12721 gtatggacag tgatgataag tttgctggtg ggaaaggaat caatgtcagc cgtgtcttga
    12781 aacgtttgaa tataccaaat acagcgacgg gatttatcgg tggctttact ggtaaattta
    12841 tcacagatac tttagcagag gaagaaatcg agacacgttt tgtccaggtg gcagaagata
    12901 ctcgtatcaa tgttaaaatc aaagcagacc aagaaacaga aatcaacgga acgggtccaa
    12961 ctgttgaatc ggttcagcta gaagaattga aagctatttt atctagtctg acagcagaag
    13021 atacagttgt ctttgcaggt tcaagtgcta aaaatctagg caatgttatc tataaggatt
    13081 tgatttcctt gacgcgccag actggtgcgc aagtggtctg tgactttgaa ggacagacct
    13141 taattgatag tttggactac cagcctcttc ttgtaaaacc aaacaatcat gaacttggag
    13201 cgatttttgg ggttaaactc gaaagtttag atgaaattga gaaatacgct cgtgagttac
    13261 tggctaaggg tgctcaaaat gttattatct ctatggctgg tgatggtgcc cttcttgtca
    13321 catctgatgg agcttacttc gctaaaccaa tcaagggaac agtcaaaaat tcagttggag
    13381 ctggtgattc tatggttgct ggattcacag gtgaatttgt caaatcaaaa gacgcagtag
    13441 aagccttcaa atggggagtg gcttgcggaa cggcaactac cttctcagat gacttggcaa
    13501 cggcggaatt tattaaagaa acatatggaa aagttgaggt agaaaaacga tgaaaattca
    13561 agacctattg agaaaagatg tcatgttgct agatttgcag gcaactgaaa aaacagctgt
    13621 catcgacgag atgattaaaa atttgacaga ccacggttat gtaacagatt ttgaaacatt
    13681 taaagaagga attttggcgc gtgaagcttt gacttctact ggtttgggtg atggaatcgc
    13741 aatgcctcac agcaaaaacg ctgctgtcaa agaagcgaca gttctatttg ctaagtcaaa
    13801 taagggtgtt gactacgaga gcttggatgg acaagcaact gacctcttct tcatgattgc
    13861 agctccagaa ggtgccaatg atactcactt ggcagccttg gcagaattgt ctcaatactt
    13921 gatgaaagac ggttttgcag acaaacttcg tcaagcaaca tctgcagacc aagttatcga
    13981 actttttgac caagcttcag aaaaaactga ggaacttgtt caagcacctg ctaatgactc
    14041 tggtgacttt atcgtagctg ttacagcttg tacaacaggt attgcccaca cttacatggc
    14101 ccaagaagcc cttcaaaaag tagctgctga aatgggggtt ggtatcaagg tcgaaaccaa
    14161 cggtgctagc ggtgttggaa atcaactaac tgcagaagat atccgtaagg ctaaagctat
    14221 tatcattgca gcagacaagg ccgttgaaat ggatcgattt gatggaaaac cattgatcaa
    14281 tcgtccagtt gctgacggta tccgtaagac agaagagcta attaacttgg ctctttcagg
    14341 agatactgaa gtctaccgtg ccgctaatgg tgccaaagct gcaacagcct ctaacgaaaa
    14401 acaaagcctt ggtggtgcct tgtacaaaca cttgatgagt ggtgtatctc aaatgttacc
    14461 attcgttatc ggtggtggta tcatgattgc ccttgccttc ttgattgacg gtgctttggg
    14521 tgttccaaat gaaaaccttg gcaatcttgg ttcttaccat gagttagctt ctatgttcat
    14581 gaaaattggt ggagctgcct ttggtttgat gcttccagtc tttgcgggtt atgttgccta
    14641 ctctattgct gaaaaaccgg gtttggtagc aggtttcgtg gctggtgcta ttgccaaaga
    14701 aggttttgcc tttggtaaaa ttccttatgc cgcaggtggt gaagcaactt caactcttgc
    14761 aggtgtctca tctggtttcc taggtgccct tgttggtgga tttatcgcag gtgccttggt
    14821 tcttgccatc aagaaatacg ttaaagttcc tcgttcactc gaaggtgcta aatcaatcct
    14881 tctattgcca cttcttggaa caatcttgac aggatttgtt atgctagctg tgaatatccc
    14941 aatggctgca atcaacactg ctatgaatga cttcctaggc ggtcttggag gaggttcagc
    15001 tgtccttctt ggtatcgtcc ttggtggaat gatggctgtt gacatgggtg gaccagttaa
    15061 taaagcagct tatgtctttg gtacaggtac gcttgcagca actgtttctt caggtggttc
    15121 tgtagccatg gcagcagtta tggctggagg aatggtgcca ccacttgcaa tctttgtcgc
    15181 aactcttctt ttcaaagata aatttactaa ggaagaacgt aactctggtt tgacaaacat
    15241 catcatgggc ttgtcattta tcactgaggg agcgattcca tttggtgccg ctgacccagc
    15301 tcgtgcgatt ccaagcttca tccttggttc agcagtagca ggtggactcg ttggtcttac
    15361 tggtatcaaa ctcatggcgc cacacggagg aatcttcgtt atcgccctta cttcaaatgc
    15421 tctcctttac ctcgtttctg tcttggtagg agcaatcgta agtggtgtgg tttatggtta
    15481 cctacgcaaa ccacaagcat aaaaaataga aaaatgaaaa gattggaccg tttggtgcag
    15541 tctttttctc ttcccgaaat gcctgtgaaa tatggtataa tagaagaatg gcaaacaaga
    15601 atacaagtac aacaagacgg agaccgtcta aagcagaact ggaaagaaaa gaagcgattc
    15661 aacgaatgtt gatttcgtta ggaattgcga ttttattgat tttcgcagcc ttcaaattag
    15721 gggctgcagg tataaccctt tataatttaa ttcgcttgct agtgggtagc ctagcttatc
    15781 tggcgatatt cggcctatta atctatctct tctttttcaa gtggatacga aaacaggaag
    15841 gactcttatc tggctttttc accatatttg ctggcttact cttgattttt gaggcctact
    15901 tggtttggaa atatggtttg gacaagtccg ttctaaaagg gaccatggct caggttgtga
    15961 cagatctgac tggttttcga acgactagct ttgctggagg gggcttgatc ggggtcgctc
    16021 tttatattcc aacagccttt ctcttttcaa atatcggaac ttactttatt ggttctatct
    16081 tgattttagt gggttctctc ctagtcagcc cttggtctgt ttacgatatt gctgaatttt
    16141 tcagtagagg ctttgccaaa tggtgggaag ggcacgagcg tcgaaaagag gaacgctttg
    16201 tcaaacaaga agaaaaagct cgccaaaagg ctgagaaaga ggctagatta gaacaagaag
    16261 agactgaaaa agccttactc gatttgcctc ctgttgatat ggaaacgggt gaaattctga
    16321 cagaggaagc tgttcaaaat cttccaccta ttccagaaga aaagtgggtg gaaccagaaa
    16381 tcatcctgcc tcaagctgaa cttaaattcc ctgaacagga agatgactca gatgaccaag
    16441 atgttcaggt cgatttttca gccaaagaag cccttgaata caaacttcca agcttacaac
    16501 tctttgcacc agataaacca aaagatcagt ctaaagagaa gaaaattgtc agagaaaata
    16561 tcaaaatctt agaagcaacc tttgctagct ttggtattaa ggtaacagtt gaacgggccg
    16621 aaattgggcc atcagtgacc aagtatgaag tcaagccggc tgttggtgta agggtcaacc
    16681 gcatttccaa tctatcagat gacctcgctc tagccttggc tgccaaagat gtccggattg
    16741 aagcaccaat ccctgggaaa tccctaatcg gaattgaagt gcccaactcc gatattgcca
    16801 ctgtatcttt ccgagaacta tgggaacaat cgcaaacgaa agcagaaaat ttcttggaaa
    16861 ttcctttagg gaaggctgtt aatggaaccg caagagcttt tgacctttct aaaatgcccc
    16921 acttgctagt tgcaggttca acgggttcag ggaagtcagt agcagttaac ggcattattg
    16981 ctagcattct catgaaggcg agaccagatc aagttaaatt tatgatggtc gatcccaaga
    17041 tggttgagtt atctgtttac aatgatattc cccacctctt gattccagtc gtgaccaatc
    17101 cacgtaaagc cagcaaggct ctgcaaaagg ttgtggatga aatggaaaac cgttatgaac
    17161 tctttgccaa ggtgggagtt cggaatattg caggttttaa tgccaaggta gaagagttca
    17221 attcccagtc tgagtacaag caaattccgc taccattcat tgtcgtgatt gtggatgagt
    17281 tggctgacct catgatggtg gccagcaagg aagtggaaga tgctatcatc cgtcttgggc
    17341 agaaggcgcg tgctgcaggt atccacatga ttcttgcaac tcagcgtcca tctgttgatg
    17401 tcatctctgg tttgattaag gccaatgttc catctcgtgt agcatttgcg gtttcatcag
    17461 gaacagactc ccgtacgatt ttggatgaaa atggagcaga aaaacttctt ggtcgaggag
    17521 acatgctctt taaaccgatt gatgaaaatc atccagttcg tctccaaggc tcctttatct
    17581 cggatgacga tgttgagcgc attgtgaact tcatcaagac tcaggcagat gcagactacg
    17641 atgagagttt tgatccaggt gaggtttctg aaaatgaagg agaattttcg gatggagatg
    17701 ctggtggtga tccgcttttt gaagaagcta agtctttggt tatcgaaaca cagaaagcca
    17761 gtgcatccat gattcagcgt cgtttgtcag ttggatttaa ccgtgcgacc cgtctcatgg
    17821 aagagcttga gatggcaggt gtcattggtc cagctgaagg taccaaacca agaaaagttt
    17881 tacaacaata aaaaaatagc ttctttccaa gtttggaagg aggctatttt agtgattatt
    17941 gattagtttt attttccgaa gttggggtat tggactgttt ttcgttttca gtagcaggtt
    18001 tacttggagt aggagcagga gtagaagaat cctgagttga tgttttctgc tcttcttttt
    18061 tctcttcctt gacgctggat tttggcgttt cttcttgttg tgttttttct tgagaagtct
    18121 tattttcctt attgggacta gtattttcct gaggggattc cttttgaatt tctttgacaa
    18181 ttgttgtcgt ctggcttgtt gtaggttcct ttttagtatt tttgttatta tccaaggcgt
    18241 tcataatcgt gatactagcg gtaatcaagc caaggatact agcgatagtc gcaacggttt
    18301 tttgaaagac tgtaaagcct tttttgatgt gttctgtttt tggttttgaa gttctacgat
    18361 aattagacat acattctctc ctttgattat gacttattat actctgtttc tttaaaaaaa
    18421 tcttaaaaaa agagaaattg ccctttcttg tcgcaagctc cgtttcatga tacaatagag
    18481 ggagtgattt tagaaagcgt gagaaacatg atttactttg ataattcagc gacgaccaag
    18541 ccttatcctg aagcccttga aacctatgtg caggtcgctt caaaaatttt aggaaaccca
    18601 tctagtctcc atcgtttggg agaccaagca acacgaatct tagatgcttc tcgccaacag
    18661 attgcagatt taatcggtaa gaaaagcgat gaaatcttct ttacctctgg tggaacagaa
    18721 ggggataact gggttatcaa gggtgtggcc tttgaaaaag ctcagtttgg caagcacatc
    18781 attgtttcag ccattgaaca tccagcagtc aaagagtcag ccctctggtt gaaaagtcaa
    18841 ggatttgaag tggattttgc tccagttgat aagaaaggct tggtcgatgt tgaggcgtta
    18901 gcaggtttga tacggcctga tacaatcctc gtttccatca tggctgtgaa caatgaaatc
    18961 ggctctatcc aacctattga ggctatttca gaattcttgg cagacaagcc gactatttcc
    19021 ttccacgttg atgcggttca ggcgcttgcc aaaattccga ctgaaaagta tctgacagaa
    19081 cgggtggatt gcgcgacctt ctcgagtcat aaatttcatg gtgtccgtgg tgttggcttt
    19141 gtctatatca aatctggcaa gaagattaca cctcttctta caggtggtgg ccaggagcga
    19201 gattatcgtt cgacaactga aaatgtggca gggattgcag cgacagccaa ggctctccgt
    19261 ttatctatgg aaaagctaga tatctttagg agcaagactg ggcagatgaa ggcagtgatt
    19321 caccaagctc ttctgaacta tccggatatt tttgtctttt cagatgagga agactttgca
    19381 cctcatattc tgacttttgg aatcaaaggt gttcgaggtg aagtcatcgt tcacgccttt
    19441 gaagactatg atattttcat ctcaacaacc tcagcttgtt catctaaggc aggaaaacca
    19501 gccggtacct tgattgccat gggagtggac aaagataagg ccaagtcagc tgtgcgtctt
    19561 agcctagact tggaaaatga tatgagtcag gtcgagcagt ttttgaccaa gttaaaattg
    19621 atttacaatc aaactagaaa agtaagatag gagcattcat gcagtattca gaaattatga
    19681 ttcgctacgg agagttgtca accaagggta aaaaccgtat gcgtttcatc aataaacttc
    19741 gtaataatat ttcggacgtt ttgtctatct atgcccaagt taaggtaaca gcagatcgcg
    19801 accgtgccca cgcttacctc aatggagctg attacacagc agttgcagaa tctctcaaac
    19861 aagtttttgg aattcaaaac ttttctcctg tttataaggt tgaaaaatct gtagaagttt
    19921 tgaagtcttc tgtccaagag attatgcggg acatctacaa ggaaggtatg acctttaaga
    19981 tttctagcaa gcgtagcgac cacaactttg aacttgatag tcgtgaactc aaccaaacac
    20041 ttggaggggc tgtattcgaa gccattccaa atgtgcaagt tcaaatgaaa agtcctgaca
    20101 tcaatcttca ggtggagatt cgtgaagaag cagcctatct ttcttatgaa accattcgtg
    20161 gggctggtgg tttgccagtt ggaacttcag gtaaagggat gctcatgttg tcaggaggga
    20221 ttgactcacc tgtagcaggt tatctagcgc ttaaacgagg ggtagatata gaggcagttc
    20281 actttgccag cccaccttac acgagtcctg gtgctcttaa aaaagcccaa gatttgaccc
    20341 gtaaattaac caaatttggg ggaaatatcc agtttatcga agttcctttt acagagattc
    20401 aagaggaaat caaagccaaa gcgccagaag cctaccttat gaccttgacg cgtcgtttta
    20461 tgatgcgtat tactgaccgt attcgtgagg tacgaaatgg tttagttatc atcaatgggg
    20521 aaagtctagg tcaagtagcc agccaaaccc ttgaaagtat gaaggctatc aatgctgtta
    20581 ccaacactcc catcattcgt cctgtggtta ccatggacaa gttggaaatc attgacatcg
    20641 cccaggaaat cgataccttt gacatttcaa tccaaccgtt tgaagactgt tgtaccattt
    20701 ttgcaccaga tcgtccaaaa acaaatccta aaattaagaa tgcggagcag tacgaagcgc
    20761 gtatggatgt tgaaggcttg gttgagcgag cagtggctgg aatcatgatt actgaaatca
    20821 cacctcaagc cgaaaaagat gaagttgatg acttgattga caatctgctc taattcagaa
    20881 aatccaaaag aatagcgaaa atcagtaaaa aaagttagtt ttttctctaa aaacaggtaa
    20941 aaaactaact ttttttattt ttatgatata atgatataaa attttgaata tagagagttt
    21001 tctgataatg aatcaatcct acttttatct aaaaatgaaa gaacacaaac tcaaggttcc
    21061 ttatacaggt aaggagcgcc gtgtacgtat tcttcttcct aaagattatg agaaagatac
    21121 agaccgttcc tatcctgttg tatactttca tgacgggcaa aatgttttta atagcaaaga
    21181 gtctttcatt ggacattcat ggaagattat cccagctatc aaacgaaatc cggatatcag
    21241 tcgcatgatt gtcgttgcta ttgacaatga tggtatgggg cggatgaatg agtatgcggc
    21301 ttggaagttc caagaatctc ctatcccagg gcagcagttt ggtggtaagg gtgtggagta
    21361 tgctgagttt gtcatggagg tggtcaagcc ttttatcgat gagacctatc gtacaaaagc
    21421 agactgccag catacggcta tgattggttc ctcactagga ggcaatatta cccagtttat
    21481 cggtttggaa taccaagaca aaattggttg cttgggcgtt ttttcatctg caaactggct
    21541 ccaccaaccg ctatatcgag tgccagaaac tatcgcctga ccaacgcatc ttcatctatg
    21601 taggaacaga agaagcagat gatacagaca agaccttgat ggatggcaat atcaaacaag
    21661 cctatatcga ctcgtcgctt tgctattacc atgatttgat agcaggggga gtacatctgg
    21721 ataatcttgt gctaaaagtt cagtctggtg ccatccatag tgaaatccct tggtcagaaa
    21781 atctaccaga ttgtctgaga ttttttgcag aaaaatggta agttaagaaa ggaaaaaacg
    21841 aaatgcatat tgaacatctt agccactgga gtggtcatct taaccgtgaa atgtacctta
    21901 accgttatgg acatggtggg attccagttg tggtctttgc ttcatcaggt ggtagtcaca
    21961 acgaatacta tgattttggc atgattgatg cctatgcttc ctttatcgag gaaggccgtg
    22021 tccagttctt taccctatct agtgtggata gtgagagctg gttggctact tggaaaaatg
    22081 ctcatgacca agcggaaatg caccgtgcct acgaacgtta tgtgattgag gaggccattc
    22141 ttttatcaag cacaaaacag gttggtttga tggcatgatg acgacaggtt gctctatggg
    22201 agcctatcat gcactcaatt tcttcctcca gcatccagat gtctttacca aagtgattgc
    22261 tctcagtggt gtttacgacg cacgtttctt tgtcggtgat tactacaacg atgatgctat
    22321 ttaccaaaac tcgccaatag attatatttg gaaccaaaac gatggctggt ttattgaccg
    22381 ttaccgtcag gcagagattg tgctgtgtac ggggcttgga gcctgggaac aagatggttt
    22441 accatccttt tacaagctca aagaagcctt tgaccagaaa caaattccag cctggtttgc
    22501 tgaatgggga catgatgtcg cccacgactg ggaatggtgg cgtaaacaaa tgccttattt
    22561 cctcggtaat ctctatttat aaaaggagtt acctatgaat taccttgtta tttctcccta
    22621 ctatccacaa aactttcaac agtttaccat cgaactagct aataaaggca tcacagtctt
    22681 gggaattggt caagagtctt acgagcaatt ggatgagccc ttgcgcaata gcttgaccga
    22741 gtattttcgt gttgataatc ttgagaacat agatgaagtc aaacgtgcag ttgcttttct
    22801 cttttataaa catggtccaa ttggccgcat cgagtctcac aatgaatact ggcttgagct
    22861 agacgcaaca ctcagagaac aattcaatgt ttttggtgcc aaaccagagg atctcaaaaa
    22921 gacgaaatat aagtctgaaa tgaagaaact tttcaaaaaa gcaggtgttc ctgtggtacc
    22981 tggagctgtt atcaagacgg aagcagatgt tgatcaagca gtgaaagaaa tcggtcttcc
    23041 aatgattgcc aaacctgata atggagtggg agcagccgca acctttaaac ttgagacaga
    23101 agacgatatc aatcacttca agcaagaatg ggaccattca accctttatt tctttgaaaa
    23161 atttgtcact tccagcgaaa tctgtacctt tgacgggctc gtggacaagg atggaaagat
    23221 tgtcttctca acaacctttg actacgccta tacaccgctt gacctcatga tttataagat
    23281 ggacaattct tattatgtgc tcaaggatat ggatcctaaa ctgcgcaagt atggggaagc
    23341 aattgtcaaa gaatttggta tgaaagaacg gtttttccat attgagttct tccgtgaggg
    23401 ggacgattat attaccatcg agtacaataa ccgccctgca ggtggtttta ccattgatgt
    23461 ttataacttt gctcattcct tggaccttta tcatggctat gcagctattg tcgcaggaga
    23521 ggagttcccg gcgtcagact ttgaaactca gtattgtttg gctacttctc gccgtgcaaa
    23581 tgctcactat gtttattcag aagaggattt gcttgccaaa tatagccagc agttcaaggt
    23641 taaaaaagtc atgccagctg ccttcgcgga acttcaagga gattacctgt atatgctgac
    23701 cactccgagt cgacaagaaa tggagcagat gattgcagat ttcggacaac gtcaagaata
    23761 agaactatcg gattaaggaa attaactccc ttaatccttt tgttttgtct gataaaaaat
    23821 aagagcatcc caacaaggta gctatcataa aacttgttcg ataactattt gaagcaggat
    23881 taggtggtca gaaattaaat tttaatattt caattgagtc atagtattgt gtttgcgtat
    23941 ccttaaatca gctaaaagga tccatgacga cacctatacg atatagtttt caagatacca
    24001 aacaagtcta ttaatattca atgaaaatca aagagcaaac taggaagcta gccgcaggtt
    24061 tctcaaaaca ctgttttgag gttgtggata gaactgacag agtcagtatc atatacctac
    24121 ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtataaaat attcaggtga
    24181 cgcatagata tagttaattg aagctttgtt tgaaatctga taaaataatg atattactaa
    24241 gttttaaaaa ctaaagaaaa gggaagatat gattacaggc gaattaaaaa ataaaatcga
    24301 tcagctgtgg gaaattcttt ggacagaagg aaacgcaaat cctttaacaa atattgaaca
    24361 gttgacttat ctcttattta tgaaagattt ggatagtgtc gagcttggac gtgaaagtga
    24421 tgctgaattt ctagggattc cttatgaggg agtttttcca aaagataaac ctgaataccg
    24481 ttggtcaact tttaaaaata taggagatgc tcaggaagtt tatcgtttaa tgactcagga
    24541 gatttttccg tttattaaaa atctcaaggg ggatacagat gatacagcct tttcacgata
    24601 tatgcgagaa gctatttttc aaataaataa acctgctacg cttcaaaagg caatttctat
    24661 cttagatgtt tttccaacta ggggattaga tgtagatttt gataatgaca aacaaagtat
    24721 tactgatatc ggagatatct atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa
    24781 tggacagttc cgtacacctc gtcacatcat cgatatgatg gttgagttga tgcaaccgac
    24841 tatcaaagat atcatctcag atcccgctat gggttctgct ggcttcttag tatctgctag
    24901 ccgttactta aagcgtaaga aagatgaatg ggaaaccaat acagataata tcaatcattt
    24961 tcataatcag atgtttcatg gaaatgatac ggatacgact atgttgagac ttggggcgat
    25021 gaacatgatg ctacatggag tagaaaatcc acaaatcagt taccttgact cgctgtctca
    25081 agataatgaa gaagccgata aatatacttt ggttttagca aatcctcctt ttaagggttc
    25141 acttgactac aattcaactt ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga
    25201 attactcttt ctttctcttt tcttgcgaac tttaaaacca ggtggacgag cagcagttat
    25261 cgtacctgat ggtgtccttt ttggttcgtc taaagctcat aaaggaattc gtcaggaaat
    25321 tgtagagaat cataagcttg atgctgtaat ctcaatgcct agtggtgttt tcaagcctta
    25381 tgctggagtt tcaactgcca ttctcatctt tacaaaaact ggtaatggtg gtactgacaa
    25441 agtctggttt tacgatatga aagcggatgg tttaagtttg gatgataagc gacaaccgat
    25501 tagcgacaat gatattccag atattatcga acgctttcat catcttgaaa aagaagcaga
    25561 acgtcagaga acggatcaat ctttctttgt tccagttgct gagataaagg aaaatgatta
    25621 tgatttgtct atcaataaat ataaagagat tgagtatgaa aaagttgagt atgaaccaac
    25681 agaggtcatc ttaaagaaaa tcaatgattt agaaaaagaa attcaagctg gcttggctga
    25741 attagaaaaa ttactcaagt agagaggtgg ctgtatgaaa aaagtgaagt tgggggaagt
    25801 cttatctcta aaaaaaggca agaaagccac tgtacttgct gaacaaacaa ctctaagcca
    25861 acgttatatt caaatagatg atttaagaaa taataataat ttaaaattca ctgaaagttt
    25921 aaatatgact gaagcactcc cagatgatat tctgatagca tgggatggag ctaatgcagg
    25981 aacagttggt tatggattat cgggagctgt tggtagtaca attacggtct taaaaaagaa
    26041 tgagcgatac aaagaaaaaa ttatatcaga ttacttggga gtctttttgg aaagtaaatc
    26101 gcagtattta cgagaacatt caacaggtgc aacaattcct catttaaaca agaatatatt
    26161 acttgattta caattagaat tgctaggtat cgaagaacaa gagaacatta tctgtattct
    26221 taatacgatt aaaaggctta ttactaaaag aaaattgcag ttagatgaac taaacttgct
    26281 cg
//
LOCUS       DAVDWM010000027        26242 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVDWM010000027 DAVDWM010000000
VERSION     DAVDWM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.027"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..248
                     /locus_tag="VUW49_001305"
     CDS             <1..248
                     /locus_tag="VUW49_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HET7981220.1"
                     /translation="NEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKS
                     YNSLFYMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            375..1334
                     /locus_tag="VUW49_001306"
     CDS             375..1334
                     /locus_tag="VUW49_001306"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET7981221.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQEKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            1431..2270
                     /locus_tag="VUW49_001307"
     CDS             1431..2270
                     /locus_tag="VUW49_001307"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7981222.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(2318..2488)
                     /locus_tag="VUW49_001308"
     CDS             complement(2318..2488)
                     /locus_tag="VUW49_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET7981223.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            2609..3493
                     /gene="miaA"
                     /locus_tag="VUW49_001309"
     CDS             2609..3493
                     /gene="miaA"
                     /locus_tag="VUW49_001309"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7981224.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            3486..4724
                     /gene="hflX"
                     /locus_tag="VUW49_001310"
     CDS             3486..4724
                     /gene="hflX"
                     /locus_tag="VUW49_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7981225.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMKELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIAFMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESITFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            4717..5340
                     /locus_tag="VUW49_001311"
     CDS             4717..5340
                     /locus_tag="VUW49_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET7981226.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            5355..6284
                     /locus_tag="VUW49_001312"
     CDS             5355..6284
                     /locus_tag="VUW49_001312"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET7981227.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGEIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGVKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            6305..7060
                     /locus_tag="VUW49_001313"
     CDS             6305..7060
                     /locus_tag="VUW49_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7981228.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(7114..8022)
                     /locus_tag="VUW49_001314"
     CDS             complement(7114..8022)
                     /locus_tag="VUW49_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7981229.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            8086..8355
                     /locus_tag="VUW49_001315"
     CDS             8086..8355
                     /locus_tag="VUW49_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET7981230.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            8355..8735
                     /locus_tag="VUW49_001316"
     CDS             8355..8735
                     /locus_tag="VUW49_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7981231.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            8824..9051
                     /locus_tag="VUW49_001317"
     CDS             8824..9051
                     /locus_tag="VUW49_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981232.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFLIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(9091..9816)
                     /locus_tag="VUW49_001318"
     CDS             complement(9091..9816)
                     /locus_tag="VUW49_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET7981233.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGDILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDCTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            9978..11819
                     /gene="typA"
                     /locus_tag="VUW49_001319"
     CDS             9978..11819
                     /gene="typA"
                     /locus_tag="VUW49_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7981234.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            11839..12093
                     /locus_tag="VUW49_001320"
     CDS             11839..12093
                     /locus_tag="VUW49_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET7981235.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            12457..12756
                     /locus_tag="VUW49_001321"
                     /pseudo
     CDS             12457..12756
                     /locus_tag="VUW49_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            12827..14848
                     /locus_tag="VUW49_001322"
     CDS             12827..14848
                     /locus_tag="VUW49_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET7981236.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNSGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRNLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            14850..15491
                     /locus_tag="VUW49_001323"
     CDS             14850..15491
                     /locus_tag="VUW49_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981237.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIERLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLIHAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            15595..16947
                     /locus_tag="VUW49_001324"
     CDS             15595..16947
                     /locus_tag="VUW49_001324"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7981238.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMAAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            16949..18007
                     /locus_tag="VUW49_001325"
     CDS             16949..18007
                     /locus_tag="VUW49_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET7981239.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            18017..19216
                     /gene="divIB"
                     /locus_tag="VUW49_001326"
     CDS             18017..19216
                     /gene="divIB"
                     /locus_tag="VUW49_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET7981240.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQKDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYISVLFNDSEQIKAFT
                     SELAQINPELKVAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            19449..20150
                     /gene="pyrF"
                     /locus_tag="VUW49_001327"
     CDS             19449..20150
                     /gene="pyrF"
                     /locus_tag="VUW49_001327"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7981241.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QLIKQATNPDFICLTPGIRPQGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            20184..20816
                     /locus_tag="VUW49_001328"
     CDS             20184..20816
                     /locus_tag="VUW49_001328"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7981242.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            20996..21399
                     /locus_tag="VUW49_001329"
                     /pseudo
     CDS             20996..21399
                     /locus_tag="VUW49_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21495..22259
                     /locus_tag="VUW49_001330"
     CDS             21495..22259
                     /locus_tag="VUW49_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981243.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            22259..23011
                     /locus_tag="VUW49_001331"
     CDS             22259..23011
                     /locus_tag="VUW49_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981244.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            23021..23719
                     /locus_tag="VUW49_001332"
     CDS             23021..23719
                     /locus_tag="VUW49_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981245.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            23716..24363
                     /locus_tag="VUW49_001333"
     CDS             23716..24363
                     /locus_tag="VUW49_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981246.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(24443..25308)
                     /locus_tag="VUW49_001334"
                     /pseudo
     CDS             complement(24443..25308)
                     /locus_tag="VUW49_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(25320..26082)
                     /locus_tag="VUW49_001335"
                     /pseudo
     CDS             complement(25320..26082)
                     /locus_tag="VUW49_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(26083..>26242)
                     /locus_tag="VUW49_001336"
     CDS             complement(26083..>26242)
                     /locus_tag="VUW49_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
                     /protein_id="HET7981247.1"
                     /translation="EVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLST
                     HLEKHWRN"
BASE COUNT         7755 a         4745 c         5786 g         7956 t
ORIGIN      
        1 tcaatgaatg ggttgatggt ggacgttatt atgttggcgc tgacggagtt tggaaggaag
       61 gtcaagcaag tacagcttct tctagtaatg atagcaatag tgaatattct gctgctttag
      121 gaaaggcaaa aagttataat tcgttattct acatgtcaaa aaaacgtatg tatagacaat
      181 taacttctga ttttgataaa ttttcaaatg atgcagctca atatgccatt gatcatttag
      241 atgattaaca gatactggta ggcgaaaaaa ttccttataa ttcatttacg caaacctttg
      301 cataagtgaa tgaattttga tatactagta ctgtaagcat tttcaattac tacacaatga
      361 aaaaggagaa tatgatgagt caaaagatta ttgggattga ccttggtgga acttctatca
      421 aatttgcaat cttgacaaca gcaggagaaa tccaagaaaa atggtctatc aagactaata
      481 ttttggatga gggaagtcat atcgtagatg atatgattga gtctattcaa caccgtttgg
      541 acttgcttgg attggcagca gcagacttcc aaggtattgg aatgggatca ccaggtgttg
      601 ttgaccgtga aaaagggact gttatcggtg cctacaactt gaactggaaa acccttcaac
      661 caatcaaaca aaagattgaa aaagctttgg gtattccatt tttcatcgat aatgatgcca
      721 acgtagcagc tcttggtgag cgctggatgg gtgctggaga taaccaacca gacgttgtct
      781 ttatgacact cggtactggt gttggtggcg gtatcgtcgc agaaggcaaa ttgcttcacg
      841 gtgttgctgg tgcagcaggt gagcttggtc acatcactgt tgactttgac cagccaatct
      901 catgtacttg tggtaaaaaa ggctgccttg agacagttgc ttcagcaaca gggattgtca
      961 acttgactcg tcgctatgcc gatgaatacg aaggcgatgc agccttgaaa cgcttgattg
     1021 ataacggaga agaagtaact gctaagactg tctttgatct cgcaaaagaa ggagacgacc
     1081 ttgctttgat tgtttaccgt aacttctcac gttacttggg aatcgcttgt gctaacattg
     1141 gttcaatcct aaacccatca acaatcgtca tcggtggtgg tgtgtcagct gcaggagaat
     1201 tccttctaca aggtgttcaa aaagtttacg atgaaaatag tttcccacaa gtacgcacat
     1261 ctactaaatt agctcttgca actctaggaa atgacgctgg agttatcgga gcagcatcac
     1321 ttgtattgca ataagataat gagagaggaa aatgctaacc tagagttagt aatgttcctc
     1381 ttttattttt tatgctatac tagttaggac aatgaatgag gtgagagtaa atgacaaaag
     1441 cagatacgat ttttaaagag aatattgaac gcattctaaa ggaaggtgtt ttctcagagc
     1501 aggctcgtcc taagtacaag gatgggactg ttgctaattc caagtatgta actggtgcct
     1561 tttccgagta cgacttatct aaaggagaat ttcctatcac aaccttgcgt cccattgcaa
     1621 tcaaatccgc catcaaagaa gttctctgga tttaccaaga ccagtcaaat agcttagaag
     1681 tgcttaatga caagtacaat gttcactact ggaatgactg ggaagttgga gacacgggaa
     1741 ccattggtga gcgctatggt gccgttgtta agaaacacga cattatcaat aagcttctca
     1801 aacagttgga aaccaatcct tggaaccgcc gcaatattat ttcgctctgg gattaccaag
     1861 ctttcgaaga aacagatggg ctgctcccgt gcgcctttca gaccatgttt gatgtccggc
     1921 gtgttgatgg ggaaatctat ctggatgcga ccttgaccca gcgctccaat gatatgctgg
     1981 tggcccacca catcaacgct atgcagtatg tggctttgca gatgatgatt gccaaacatt
     2041 ttggctggaa ggttgggaag ttcttctact tcatcaacaa cctccatatc tatgataatc
     2101 aatttgaaca agctcaggaa ttgctccgtc gggagccgtc aaactgccaa ccacgcttgg
     2161 ttttaaatgt tcctgatggg actaatttct ttgatatcaa agcagaagat tttgagttgg
     2221 tggattatga ccctgttaag ccacagttga agtttgacct agctatttaa aagaatagaa
     2281 aaaagaagtt gagaataatc ccaacttctt ttgtttctta acgtgatacg cggcgacgag
     2341 ctgctttttt acggttttct tcgatgaaag ctgctttttg ctcttctggt tcgattactt
     2401 tctttttaaa tgcgtatact gcacctgcaa cggcagcgac agttcctgcg acacctgtta
     2461 caagaccttt agcgaatcct ttagccatga gtcttcctcc tttatattct caatcagcca
     2521 gcctcctcaa gaggtcacat ttttctgact gacctttttg tgttataata atagtaacga
     2581 aaaaatggga atttttcaag gaaaaaagat gaaaacaaaa ataattgtga ttgttggacc
     2641 gactgctgtt ggaaagacgg ctctagctat tgaagttgca aagcgtttta atggcgaagt
     2701 ggttagtgga gatagccagc aagtctatcg aggacttgat attgggacgg ccaaggctag
     2761 tccagaagag caggcagctg ttcctcatca tttaatcgat gttagagaga taaccgagtc
     2821 ttactcggct tttgattttg tttcagaagc taagatgact attgagggta ttcacaaccg
     2881 tggcaagcta gccattatcg ccggtgggac tggactttat atccagagct tgctagaagg
     2941 ttaccaccta ggtggggaga ctcctcatga ggagatttta gcttatcgag ctagtttgga
     3001 gccatattca gatgaggaat tagcccattt ggtggatcaa gcaggccttg agattcccca
     3061 gtttaatcgt cgtcgtgcta tgcgtgcctt agaaattgcc cattttggtc aggatttgga
     3121 aaatcaagag accttgtatg aaccgctgat tatctgcttg gatgatgaac gtagtcaact
     3181 ttatgagcgt atcaaccacc gagtggattt gatgtttgag gctgggcttt tggatgaggc
     3241 caagtggctg tttgaccatt cccctaatgt acaggctgct aaaggaattg gctataagga
     3301 actctttcct tatttccgtg gagagcagac cttggaggaa gctagtgaga gtctcaaaca
     3361 ggcgacccgt cgttttgcca agcgtcagct gacctggttc cgtaatcgca tgcaggtcac
     3421 cttttatcag attggagaat ctggtgtaca agaccgcatt ttaagccaga tagaggagtt
     3481 tttagatgat tgaaacggag aaaaaagagg agcgagtcct gctgattggt gtggaattgc
     3541 agggtatgga cagttttgac ctctccatga aagaattggc tagtttagcg aaaacggcag
     3601 gggcagtcgt tgtagatagc tacagacaaa aacgtgaaaa atatgattcc aagaccttcg
     3661 tcggctctgg taagttggaa gagattgcgt ttatggtgga tgcagaagaa atcactactg
     3721 tcatcgtcaa caaccgtctg accccaaggc agaatgtcaa tctagaggaa gttctcggtg
     3781 ttaaggtcat tgaccgtatg cagttgattt tggatatctt tgccatgcgg gctcgaagcc
     3841 atgaagggaa gctccaagtc cacctagccc aactcaaata cctcttgcct cgcttggttg
     3901 gtcaggggat tatgctcagc cgtcaggcag ggggaattgg ttcccgtggt cctggtgaaa
     3961 gccaactgga gctgaaccgt cgtagcgttc gcaatcaaat cacggatatc gagcgccagc
     4021 tcaaggtggt tgagaaaaat cgtgcgactg tcagagaaaa acgtttggag tctatcactt
     4081 ttaagattgg tttgattggt tataccaatg ctgggaaatc aactatcatg aacatcttga
     4141 ccagtaagac ccagtatgaa gcagatgagc tctttgcgac tctggatgcg acaaccaaga
     4201 gtattcatct gggaggcaac ctccaagtaa ctttgacaga taccgttggc tttatccaag
     4261 atttgccgac agagttggtg tccagtttca agtcaacctt ggaagaaagc aagcatgtgg
     4321 accttctggt tcatgttatc gatgctagca atccttacca cgaggaacat gaaaaaacgg
     4381 ttctctccat catgaaagac ctggacatgg aagatattcc tcacttgacg ctttataata
     4441 aagcggattt ggtggaggat ttcacgccta cccaaacgcc atataccctc atttctgcca
     4501 agtctgagga cagtcgtgaa aacttgcaag cattattgct agataagatt aaggaaattt
     4561 ttgaagcatt taccctgcga gtgccttttt caaagtccta caagattcat gatttagaga
     4621 gtgttgcaat tctggaagaa cgtgattatc aggaagacgg cgaagtgatt acaggctaca
     4681 tttccgagaa aaataaatgg aggttagaag aattttatga ctgatattaa aactttggct
     4741 ctaaagtatg gaggttatac aagtctggac aaggtctatc tggatcagct tctagctggt
     4801 aagacagagc aggagcagtt aacacttatt acacctccgc caagtgttgt taatgcctac
     4861 tttgcagaac tctatcagaa aaaaagtcct caggctgcga cggattattt tgcagaactc
     4921 agtcaggaac tgaatcttta caatactgag ccaagtttca ccctagaaaa taagtctttt
     4981 attcggctta atctgtctgg taaatccttt ggtttttgtt atgagagtga cggctttggt
     5041 cggattttct ctgaaaatga ggagaaaatc tcggatgact tgctctttga aattgcgcaa
     5101 attttccccc atcaactagt ctttgaagag tctggtaaga tttacatgaa gcctgtcgag
     5161 gacgaggaag ttgtcagcct agaaagtctc acagctttga ctgatttgga aagattggct
     5221 gatggtcgaa aacgtctcaa aggctacagc caagaggatt tattgcaaga agctgctgct
     5281 ttttctggca aacgctattt ccgatcggaa aaccgcacag ccatgttata tattgattaa
     5341 ttagaaagta tcgaatggat attcaatttt taggaacggg ggctggtcag ccctctaaag
     5401 ctcgcaacgt ttcaagtctc gccctgaaac ttttggacga gattaacgaa gtttggctct
     5461 ttgactgtgg agaaggtacg caaaatcgca ttctggaaac cacaattcga ccacgtaagg
     5521 tcagcaaaat ctttattacc catctgcatg gagaccacat ttttggtttg ccaggtttcc
     5581 tttctagccg tgcctttcag gccaatgaag agcagacaga tttggaaatc tacggacctc
     5641 aaggaatcaa gtcatttgtc ttaaccagcc ttcgtgtgtc aggttctcgt ctgccctacc
     5701 gcattcattt ccatgagttt gaccaagatt ctctgggtaa aattcttgaa accgataaat
     5761 tcactgtgta tgcagaggag ctggaccaca ctattttctg tgttggctat cgtgtcatgc
     5821 aaaaggatct agaagggacg ctggatgctg aaaaacttaa ggctgctggt gttccgttcg
     5881 gcccgctttt tggtgaaatc aaaaacggcc aggatcttgt tttggaagac ggaactgaaa
     5941 tcaaggcagc agactatatc tcagcgccac gtccaggtaa gattatcact attttaggag
     6001 atactcgaaa aacggatgcc agtgtgcgtc tggctgtcaa tgcagatgtc ctagttcatg
     6061 agtccactta tggcaagggt gatgaaaaaa ttgctcgtaa ccatggtcac tcaactaata
     6121 tgcaagctgc acaagtagcg gtagaagcag gtgtcaaacg cctcctactc aaccatatca
     6181 gtgcccgttt cctctcaaaa gatattagca aactcaagaa agacgctgcc acaatttttg
     6241 aaaatgtcca tgtggtcaaa gacttggaag aagtggaaat ctagcagtca cagaaaggat
     6301 aagtatgcct actattctca ttaccggagc tagcggtggt ctagctcaag aaatggtcaa
     6361 actcctgccc aatgaccaac tcatcttgct tggtagaaat aaggaaaaat tagcccaact
     6421 ctacggaaat tattcccatg cagaattgat tgaaattgat attaccgacg attcagccct
     6481 agaagctctg gtaactgatc tttatctccg ctatggcaag attgatgtct tgattaacaa
     6541 cgctggttac gggatttttg agggatttga ccaaattgct gataaagaca ttcaccagat
     6601 gtttgaggtc aatacctttg ccctgatgaa tctgtctcgt caccttgcgg ctcgtatgaa
     6661 ggaaagcagc aaagggcata tcatcaacat cgtcagcatg gcaggtctaa tagctactgg
     6721 caagtctagt ctttactcag cgaccaagtt tgcggctatt ggtttttcaa atgctctgcg
     6781 actcgaactt atgccctatg gagtctatgt gacaacagtc aatccaggtc caatccgaac
     6841 aggatttttt gaccaagctg acccagatgg aacttatctt aaatcggttg accgcttcct
     6901 cttagaggca gatgcagtgg ctaaaaagat tgtcaagatt ataggaaaaa ataaacgaga
     6961 actcaatctc ccgattttgt tgaacctagc ccataagttt tatactctct ttcccaagct
     7021 agctgataag ttggcagggg aaacttttaa ttataagtaa aaagaaccaa tgtgcaggtt
     7081 gttgctagcc tacatattgg ttcttatcat ttctcaacta tcaaactgaa cttcttctag
     7141 tagatagtcg ataaagcgtt cgcccatctt agataggctg gttttctcat gctggatata
     7201 gaccagctca atcgggtcat caatatccag tgggatagaa acgatattgt ctccgtttag
     7261 gttgctgttc aaaatccctg tcgcaatggt ataaccatcc aaaccaatca agagattaaa
     7321 gagggtggca cggtcactga ccacaatgga tttcttgtgg tgttcttgag aaagaatctc
     7381 ttctgaaaag tagaaggagt tgtgcgtccc ttggtcatag ctgaggtaag ggaaattctc
     7441 caaatcagac agtttcacct tgtctttctt tgccagaggg ttggtcttgc tgacaaagat
     7501 atgcggttgc gctgtgaaga gatggtgggc tagcaggtga ttgtcatcca gcatcttggt
     7561 taaaacatca cggttgtaac tgtttaagaa gaggaccccg acctcactgc ggaagttctt
     7621 gacgtcgtcg ataatctccc aagtccgagt ttcacgaagg aagagttcgt atttctccat
     7681 atcgcttttc ttgagcaaag agacaaaggc attgaccaca aaggcatagt gttgagacga
     7741 aacgctaaag agttcgcggt gggcgacagg atttttatag cgttcctcca gaagctgggt
     7801 ctgctcgaca acctgacggg cataagagag aaactccatc ccatcacggg tcaaggtgat
     7861 tcccttggga ttgcggataa agatctcaat gcccatttca ttttccaaat ctcgcactgc
     7921 attggagaga cttggctgag tgataaagag ttgcttggct gcctcattca tggagccagt
     7981 ttcgacgatt ttgataatat aatgtaattg ttgaattctc atgtttttat tgtaccatac
     8041 ttgccttaga atcgctaaag aagataggaa aataaggagc attgtgtgag tctcttcttg
     8101 aaaaaaacac aaaattttgc tagaatgagt cttatgaaaa cattctatga tgtgcagcaa
     8161 ttcctcaaac gatttggtat tattgtttac atgggaaaac gcttatatga tattgaactg
     8221 atgaagttgg aactatctcg gatttacgat gcagggttga tggataaatt agactatcta
     8281 gaggcagaag cagttcttcg cagagagcac aaggtagaat tggattatat tgagaaaaat
     8341 ggagaaaaga actaatggtt acttggattt tgtgggcact tatactagca atgttggcgt
     8401 ggatgggctt taactatctt cgtattcgcc gtgcggctaa aattgtggac aatgaggagt
     8461 ttgaagcctt gattcgtacg ggtcaattga ttgatttgcg cgacccagca gaattccaca
     8521 gaaaacatat ccttcgtgca cgcaatattc cttcaagtca gttgaaaact agtcttgcag
     8581 cccttcgtaa agataaacct gtccttctct acgaaaacca acgtgcgcaa cgagttacaa
     8641 atgcagctct ttacttgaaa aaacaaggtt tttctgagat ttatatcctt tcttatggct
     8701 tggattcttg gaaagggaaa gtgaagacta gctaacctta tttccaaatt agtcgaatga
     8761 taggatgaag ctattgtttt atagtataat taatttgtat aaaatttaaa gagttcttag
     8821 aacatgaaag aaaagaagaa aaatccactt tttgtgggta tcttgtcgat tattcttggt
     8881 ttgttatttc taattgtagg cctgattttg ggtatcatag gattggtctt ggctatttca
     8941 taccaaaaag agtcacaatt agactataaa atagaaaaga ttcttaatat cttaggaatt
     9001 gtgatttctg tagttaactg gattgtagca atcgccttaa tttttcggta aataaaagaa
     9061 aaaagcttta gttttaaagc tttttttatt ttatcgactc atctctaaat agtttttaat
     9121 gatttgtaac tctttttggt tcaaagggcg gtagttacct tctgctaaat ctgggttcaa
     9181 tgtgaagtcc ccaaattgga ttctcttgag gatagtcacc ttaacaccaa ccgagaggaa
     9241 cattttcttg atttgatgaa attttccttc tgaaatggtg atagaggctt gactgatgga
     9301 gggacttgca gatagaatct ctagttttgc gggtttacag acagtacaat ctaaaaagac
     9361 aattcccttt tgaaaggctt ggatatggtc aggtgttaga agtccattaa cctcaacttg
     9421 ataagtctta tcgacatgat attggggatg gaggagttgg aagcccaagg gaccgttatc
     9481 ggtcaagaga aggagtcctg ttgtatctcg gtccagtcgg ccgacggcat agagcttgtc
     9541 agactggatg tcagatggaa gcaagtccat gacggtcgga agttccttgt ctttgttggc
     9601 tgtaacgaca ccagcaggtt tatgaagcat aagataggtg tgttcatagc cttgaatgat
     9661 tcgatcctga aaaaggagtt cttgtagtcc tgtatcgata ttttgagcta gggagcgggc
     9721 tgggcaacca tcgactagaa tgtcccctct gaggagtgct tgcttcatgg cctttcggct
     9781 aactttttct tgggctaata aattatctaa acgcatactg tgcctatctt atcataagtc
     9841 gcttgctttg aaaaggcttg acgtgaaaag aaaagcggag tgaaaacatt tacacttgct
     9901 tgagttatgt tatttatttg aaattatggt ataatcgttc agttagaaaa taaattttga
     9961 atattataga ggaaatcatg acaaaattaa gagaagatat ccgtaacatt gcgattatcg
    10021 cccacgttga ccacggtaaa acaaccctgg ttgacgaatt attgaaacaa tcagaaacgc
    10081 ttgatgcacg tactgaattg gcagagcgtg ctatggactc aaacgatatc gaaaaagagc
    10141 gtgggattac catccttgct aaaaatactg ccgttgctta caacggaact cgtatcaaca
    10201 ttatggacac accaggacat gcggacttcg gtggagaagt tgagcgtatc atgaaaatgg
    10261 ttgacggtgt tgtcttggtc gtagatgcct atgaaggaac catgccacaa actcgtttcg
    10321 tattgaaaaa agccttggaa caagaccttg tcccaatcgt ggttgttaac aaaatcgata
    10381 agccatcagc tcgtccagca gaagtagtgg atgaagtctt ggaacttttc atcgagcttg
    10441 gtgcagatga cgaccagctt gatttcccag tggtttatgc ttcagcgatc aacggaactt
    10501 cttcattgtc agatgatcca gctgaccaag aagcgactat ggcaccaatc tttgacacga
    10561 ttatcgacca tatcccagct ccagtagata actcagatga gcctttgcag ttccaagtgt
    10621 cacttttgga ctacaatgac ttcgttggac gtatcggtat cggtcgtgtc ttccgtggta
    10681 cagttaaggt tggggaccaa gttacccttt ctaaacttga cggtacaact aaaaacttcc
    10741 gtgttacaaa actcttcggt ttctttggtt tggaacgtcg tgaaatccaa gaagccaaag
    10801 cgggtgactt gattgccgtt tcaggtatgg aagacatctt tgtcggtgaa accatcactc
    10861 cgacagatgc agtagaagct cttccaatcc tacacatcga tgagccaact cttcaaatga
    10921 ctttcttggt caacaactca ccatttgctg gtaaagaagg taaatgggta acttctcgta
    10981 aggtggaaga acgcttgcag gcagaattgc aaacagacgt ttcccttcgt gttgacccaa
    11041 ctgattcacc agataaatgg actgtttcag gacgtggaga attgcacttg tcaatcctta
    11101 tcgaaacaat gcgtcgtgag ggctatgaac ttcaagtatc tcgtccagaa gttatcgtaa
    11161 aagaaatcga cggtgttaaa tgtgaaccat ttgaacgtgt acaaatcgac actccagaag
    11221 aataccaagg gtctgttatc caaagccttt ctgaacgtaa aggtgaaatg ttggatatga
    11281 tttcaactgg taatggtcaa actcgtttgg tcttccttgt tccagcgcgt ggtttgattg
    11341 gatactcaac tgagttcttg tcaatgactc gtggttacgg tatcatgaac cataccttcg
    11401 accaatactt gccattgatt ccaggggaaa ttggtggacg tcaccgtggt gcccttgttt
    11461 ctatcgatgc tggtaaggct acaacttact caatcatgtc tatcgaagaa cgtggtacga
    11521 tctttgtcaa cccaggtact gaggtttatg aaggaatgat catcggtgaa aactctcgtg
    11581 aaaatgactt gacagttaac atcacgaagg caaaacaaat gaccaacgtt cgttcagcta
    11641 ctaaggacca aacagctgtt atcaagacgc ctcgtatctt gacacttgaa gagtctcttg
    11701 agttcttgaa cgacgatgag tacatggaag taacgcctga gtctatccgt ttgcgtaaac
    11761 aaatccttaa caaagcagag cgtgagaaag ctaacaagaa gaaaaaatca gctgaataag
    11821 agctagaaag agagaaagat ggtctattta gtcctaggaa ttttactgct cctactctat
    11881 gtatttgcga caccagaaag cattaaaggg actgtcaata tcgtcgctat ggtatgtatt
    11941 ttagtggcac tcttgatttt attggttcta tcttttctga aaatttttca attaccaaca
    12001 gaaatattcc tagcaatagc catgttgatc ctagcttact ttagtgttag agacatcaca
    12061 ctcatgccag tcaaaaaaag taaaagaaga taaaaaagag agtttcggct ctctttttgc
    12121 taggaatttt acaatagttt gaattgcttt ttagttaaca ccatctcttt tacttgtata
    12181 gtatgaatgg gaaaaacttg aataaaattc gttaatggca tctattttat atttggttag
    12241 tactggttgc atattatttc gaaataaaac tactatataa tattattcac agaggtaata
    12301 tagtaaaata tctaaaaaat tcgatttttt ccttgacaac aaataataaa aaagttattc
    12361 ttatcacata agaaagcggt ttcaataaaa gatagaggag gatttatttt gtttcattgt
    12421 tgctcaaggt gtgctttttt tgaaagaaaa aataaaatga aaacgaaaaa acatagatta
    12481 cttgctctag ctcttatttc aaattttaca ttattgggag ctgcatcagc tgctgtacaa
    12541 tatccagatg gaggagtatg gacatatgga gaaggttcag gaggtggtta ggctttttca
    12601 aattactatc atggtaaaaa atatcattat tcatccatcg tgagcagatg ggatggtcat
    12661 tctgataaag gagaagctcc tgctggaaaa acctcctatg catggatttg gactaaatgg
    12721 ggagaacaag tagcatttta ctgtgattat gactaatcgt tcattgattt aaatcgttcc
    12781 ccttgtaaga aattacaggg ggaattttgt ttacaaggag tctactatga agaaactatt
    12841 catattatta tcaacttttt ttctcagctt cttccttgct tggattattg tcttacgtgc
    12901 gccacaatat ttatatgcaa gctatgattc cgtttcttta cttcgtgtca aaaaagatac
    12961 tcaggaaccg acgcgtgagg tatttgaaca ggaattggag aattttgcaa actcagaaca
    13021 gagtttaata gctagaagaa ttgtagagcc gagtaaggat gggacgactc actttactta
    13081 tgcaacttat ggtcagggaa ctttaccaaa agaattccaa gaagctagcc aagaaagtcg
    13141 tgaacgtagt gatccgctaa atagttatct ccttttgtca ggctccttga cgaaagaaaa
    13201 gcttgccgat aaattaggag atttgggtta taaagcaagt gctgaccgaa agataccgcc
    13261 ctattttctt gcttttcgaa tattactaaa tccccttatt ttaattagtt tagcaatatt
    13321 tggtttatct ttctttgctt tagtgattat cactcggatt aaggaaatga gagcagcagg
    13381 tataaaactc ttttctggtc agactctctt atccatcatg gggcattctt tatctactga
    13441 tatcaaatgg ctccttctat cagccctcct ttccttccta ggtgggggag tcgttctttt
    13501 tagtcaaggt ttgttttatc ctatcttgtt agccacctat ggttttggga ttagtttcta
    13561 tctgttgttt ttattggcga tttcaatttt actaatgctt ctttatctaa tgagtttgaa
    13621 ttacaaagca ttagttcccg ttattaaggg gagattaccc cttaaacgcc tgatgatttt
    13681 aaccctattg tgtcagttgg ttgctgtctt tacagtagga tacgctgtta agacaggttt
    13741 gacgtcttac caacgattga aagaacttga aatttcaaaa caagcatggc aggatagagc
    13801 agactattat caaatttctt ttggcttagg tgatagagga aaagatacag aaaatcagag
    13861 caagtggtat gcctttgcca aggaagcaat cgaagaagaa caagctcttt atgtaaagga
    13921 taatctgctc cattttgcca atccacaagg aaaaaatgaa cagggagaga cactggatac
    13981 ctatagtcca gatgctaata cgctctatgt tagtcccagt tatttggaca aggaaaaggt
    14041 cgtggtagat gctgagacca aacagaagtt agcccatctc caaaaaggtg agtttatcct
    14101 cttgctccca gaacatttgc gctctcgaga agcagaactt aagaaagttt ttgaagaaag
    14161 attgagttat tatggaaaat ctggtgagga ggcaagtgct cctttggatt atgagatgaa
    14221 agcgcacgtt agttatcttt caatgggaga aaagcggttt gtttataata gcggtgagaa
    14281 tcccgtatct actcagtatt tgactgatcc gattttagtg gtattcacgc cgacttctac
    14341 aggtgatagt tttatatcct tatctagttg gtctatcaat gctggaaaac aactctttat
    14401 caaaggatat gagagtgggc tagaactctt gaagaaagct ggaatttatg agcaagtatc
    14461 ctatcttaaa gaaggaagaa gtgtttatct aactcgttat aatgaagttc aaactgaaac
    14521 agcaacttta atcttaggag ctattgttgg aatagccagt tccttgttac tcttttattc
    14581 tgtcaatctt ctatatttcg agcaattccg ccgagatatc ttgattaaac gaatttcagg
    14641 tttacgattt tttgaaacac atgctcagta tatggttagc caatttgcca gttttgtatt
    14701 tggtgctagt ctctttattt taagcagtcg aaacttggtg attggcttgc tcactttatt
    14761 ggtctttcta gctagtgcag ttttgacgct ttaccgtcaa gcgcagaaag aatctcgtgt
    14821 ttctatgaca attatgaaag gaaaatagga tgattgaact aaagaatata tctaaaaaat
    14881 ttggaagccg tcagctattt tcagatatga atcttcattt tgaaggtggg aaaatttatg
    14941 ccttaatcgg tacaagtggc tgtggtaaga caacactctt gaatatgatt gaacgattag
    15001 agccatatga caaagggcaa atcatatatg atggcacttc tcttaaggac attaagcctt
    15061 ctgttttctt tagagattac ttaggatact tatttcaaga ttttggctta attgaaagcc
    15121 aaaccgtcaa agagaatctc aatctgggtt tagttggtaa aaagttgaag gaaaaagaga
    15181 aaatctcttt gatgaaacaa gctctaaacc gtgtaaacct ctcttatttg gatttgaagc
    15241 aacctatatt tgagttatca ggaggagaag cacaacgtgt tgcactagcg aagataattt
    15301 taaaggatcc acctttgatc cacgcagatg aaccaaccgc ttccttagac cccaaaaact
    15361 ctgaggaatt actttccatc ctagaatctt taaaaaatcc gaatcggacc attattattg
    15421 cgacccacaa tcctctgatt tgggagcaag tggatcaggt cattcgagtt accgatttat
    15481 cacatagatg atatggtaag attcagttag aagaaagagt cacaaacaca ctttgtggct
    15541 tttttatttc cataaaaatg gtaaaatagt aggagtagaa atggagttcg agacatgaaa
    15601 gtaatagatc aatttaaaaa taagaaagtt cttgttttag gtttggccaa gtctggtgaa
    15661 tctgcagctc gtttgttgga taagctaggc gccatagtga cagtaaatga cgggaaacca
    15721 ttcgaggaca atccagctgc gcaaagtttg ctggaagaag ggatcaaggt cattacaggt
    15781 ggccatcctt tggaactctt ggatgaagag tttgccctta tggtgaaaaa tccaggtatc
    15841 ccctacaaca atcccatgat tgaaaaggct ttggccaagg gaattccagt cttgactgag
    15901 gtggaattgg cttatttgat ttcagaagca ccgattattg gtatcacagg atcgaacggt
    15961 aagacaacca caacgactat gattggggaa gttttgactg ctgctggcca acatggtctt
    16021 ttatcaggga atatcggcta tccagctagt caggttgctc aaatagcatc agataaggac
    16081 acgcttgtta tggaactttc ttctttccaa ctcatgggtg ttcaagaatt ccatccagag
    16141 attgcggtta ttaccaacct catgccaact catatcgact accatgggtc attttcggaa
    16201 tatgtagcag ccaagtggaa tatccagaac aagatggcag cagctgattt ccttgtcttg
    16261 aactttaatc aagacttggc aaaagacttg acttccaaga cagaagccac tgttgtacca
    16321 ttttcaacac ttgaaaaggt tgatggagct tatctggaag atggtcaact ctacttccgt
    16381 ggtgaagtag tcatggcagc gaatgaaatc ggtgttccag gtagccacaa tgtggaaaat
    16441 gcccttgcga ctattgctgt agccaagctt cgtggtgtgg acaatcaaac catcaaggaa
    16501 actctttcag ccttcggtgg tgtcaaacac cgtctccagt ttgtggatga catcaagggt
    16561 gttaaattct ataacgacag taaatcaact aatatcttgg ctactcaaaa agccttgtca
    16621 ggatttgaca acagcaaggt cgtcttgatt gcaggtggtt tggaccgtgg caatgagttt
    16681 gacgaattgg tgccagatat tactggactc aagaagatgg tcatcctggg tcaatctgca
    16741 gaacgtgtca aacgggcagc agacaaggct ggtgtcgctt atgtggaggc gacagatatt
    16801 gcagatgcga cccgcaaggc ctatgagctt gcgactcaag gagatgtggt tcttcttagt
    16861 cctgccaatg ctagctggga tatgtatgct aactttgaag tacgtggcga cctctttatc
    16921 gacacagtag cggagttaaa agaataatat gaaaaaaatt gtctttacag gtggggggac
    16981 ggttggacac gttaccctca atcttttgtt aatgcccaag ttcatcgaag atggttggga
    17041 agtccactat atcggggaca agcgtggtat cgaacaccaa gaaatcctta agtcaggttt
    17101 ggatgtcact ttccactcca ttgcgactgg gaaattgcgt cgctatttct cttggcaaaa
    17161 tatgctggac gtcttcaaag ttggctgggg aatcgtccaa tcgctcttta tcatgttgcg
    17221 acttcgtcca cagacccttt tttcaaaggg gggctttgtc tcagtaccgc ctgttatcgc
    17281 tgcgcgtgtg tcaggagtgc ctgtctttat tcacgaatct gacctgtcta tgggcttggc
    17341 caataaaatc gcctataaat ttgcgactaa gatgtattca acctttgagc aagcttcgag
    17401 tttgtctaag gttgagcatg tgggagcagt gaccaaggtt tcagatcaaa aaaatccaga
    17461 accagatgaa ttggtggata ttcaaagcca ctttaatcat aaattgccga ctgtattgtt
    17521 tgttggcggt tctgcaggtg ctcgtgtctt taaccaattg gtgacagacc ataaaaaaga
    17581 actaacagag cgctacaata ttatcaatct aactggagat tctagcctga atgagttgag
    17641 ccaaaatctt tttcgtgttg actatgtgac cgatctctat caacccttga tggaattggc
    17701 tgatattgtt gtgacacgag gtggtgccaa tacgattttt gagctcttgg cgatagcaaa
    17761 attgcatgtc attgtgccgc ttggtcgtga agctagtcgt ggtgaccaga ttgaaaatgc
    17821 agcttacttt gtaaaaaaag gctatgcaga agaccttcaa gaaagcgatt tgaccttgga
    17881 tagtttggaa gagaagcttt ctcacttact aagtcacaag gaagattacc aagctaagat
    17941 gaaagcttct aaggaattga aatctctagc agatttttat caattgttga aaaaagattt
    18001 atcataagga aagtaaatgt caaaagataa gaaaaatgag gacaaagaaa ccctcgaaga
    18061 attgaaagag ttatcagaat ggcagaaacg aaaccaagaa tatctaaaaa agaaggctga
    18121 agaagaggcg gctctagctg aggagaagga aaaggaaaga caagctcgaa tgggagaaga
    18181 atctgagaag tcagaggata aacaggacca ggagagtgaa acagaccaga aagattcaga
    18241 atcagctaag gaagagtctg aagaaaaagt agcatcctca gaggctgaca aagagaaaga
    18301 agagaaagaa gaaccagagt ctaaagagaa ggaggaacag gataaaaagc tttctaaaaa
    18361 ggctacaaag gaaaaaccag ccaaagcaaa gattcctggt atccatatct tgcgagcctt
    18421 cacgatttta tttccaagtc tgcttttatt gattgtctct gcctacttgc tcagtcctta
    18481 tgcgaccatg aaagatattc gtgttgaggg aacggtgcaa actacagctg atgatattcg
    18541 acaggcttca ggcattcagg attcggatta tacgattaac cttctgctag acaaggcaaa
    18601 atatgaaaag cagattaagt ctaactattg ggttgaatca gctcaacttg tctatcaatt
    18661 tccaactaag ttcactatta aggtcaagga atatgatatt gtggcctact atatttctgg
    18721 tgaaaatcat tatcctattc tttccagtgg tcagcttgag actagttctg tgagtctgaa
    18781 cagtttacca gaaacttata tatcagttct ctttaatgac agcgaacaaa tcaaagcctt
    18841 tacgtcagaa cttgctcaaa ttaacccaga actcaaggtg gctatccaaa aggtggaatt
    18901 agccccaagc aaggtgactt ccgatttaat tcgattgact atgaatgatt cggacgaagt
    18961 cttggttccg ctttctgaaa tgagcaagaa actgccttat tatagtaaga ttaagccaca
    19021 attgtcagaa ccgagtgtga ttgacatgga agctggaatt tacagttaca ctgtggcgga
    19081 taaattaatt atggaagctg aggaaaaagc caaacaagag gctaaggaag ctgagaaaaa
    19141 acaagaagaa gaacagaaaa aacaagagga agagagcaat cgaaaccaaa cgacccagcg
    19201 ctcatcgcgt cgctaggttt accttttctc ttatagttct ttagtgacca tgtttttact
    19261 tgacaagtgc tagtagaaat aatagaataa tagaaaacct ttaaagcagt ccagagaggc
    19321 agctaaggtt agacggtgaa agggtggaga ctacccattt ttcgtggaac cttgctgttg
    19381 gcaggttcct tttttcgtgg cttctgttgg ccagactctc tcactagtaa aggtaaaagg
    19441 agaaacctat gcgagaacat cgtccaatca ttgctcttga ttttcctagt tttgaggcgg
    19501 tcaaggaatt tttagctctt ttcccagcag aagaaagcct ttatctcaag gtagggatgg
    19561 agctttatta cgcagcgggg cctgagattg tgtcctactt aaaaggtttg ggtcatagtg
    19621 tctttttgga tctcaaactt catgacattc ctaatacagt caagtcagcc atgaaggtct
    19681 tgtctcagct tggtgtcgat atgaccaatg ttcatgcggc tggtggtgta gagatgatga
    19741 aggctgcgcg tgaaggtctt ggaagtcaag caaaattgat cgctgtaacg cagctgacat
    19801 caacatcaga agcccagatg caggagtttc aaaatatcca aacaagccta caagagtctg
    19861 tgatccacta tgccaagaag acagctgaag caggcttgga tggtgtcgtt tgctcggcac
    19921 aggaagttca acttatcaag caggccacca atccagattt tatctgtctg acaccaggga
    19981 ttcgcccaca gggagctgct gttggagacc aaaaacgcgt catgacgcca gcagatgctt
    20041 atcaaattgg tagtgactat atcgtagtgg gacgtcccat tacccaagct gaggatcctg
    20101 ttgcagctta tcatgccatc aaggatgaat ggacacagga ctggaattaa agaactagat
    20161 tagaaaaata aaaggagaat accatgacac ttgctaaaga tatcgctagc cacctcttga
    20221 aaatccaagc cgtttacctc aaaccagagg aacccttcac ttgggcgtct ggtatcaagt
    20281 caccgattta cactgataat cgtgtgacac tagcctatcc agaaactcgt accctaattg
    20341 aaaatggttt tgtggaagct atcaaagaag cctttcctga agtagaagtg attgcaggaa
    20401 ctgcaacagc agggattcca cacggagcca ttattgctga taagatggac ttgccttttg
    20461 cctacatccg tagcaaacca aaagaccacg gagctggtaa tcaaatcgaa ggtcgcgtag
    20521 ctcaaggtca aaaaatggta gtggttgaag accttatttc aacgggtggt tcagttcttg
    20581 aagctgtagc agcagccaag cgagaaggag cagatgtact tggagttgta gcgattttca
    20641 gctaccaatt gccaaaagca gataagaact ttgcagatgc tggtgttaaa cttgtgacgc
    20701 tttcaaacta tagcgagctt atccatctag cccaagaaga aggttacatc acgccagagg
    20761 gccttgatct tctaaaacgc tttaaagaag accaagaaaa ttggcaagaa ggttaggtca
    20821 gtaagataaa gagagacgag gctaccgagt ctcttttacc attttattta aaatatgata
    20881 atattttaaa taaaataatt gacaacgctt tcttttattg atataatgaa aatgtaatat
    20941 gtatcaaatt gatcaatcaa ctatcttgtt tatcacttct taagaggacg atgtcatgaa
    21001 aagaagaaaa atatattcct tttttgcgta gttacaattg catctagttt gtttctccct
    21061 acagttttag ctagacagat tggaaattat gatttaacgt tgccgcgttg gggttcggat
    21121 acaactagtg agcttgagaa agaaaacgcc tctgctggaa ttaataatag tgacagcact
    21181 ggtggcggta aaaggttaaa tacctctatt cgtagcgcct atagtgggtc agatattacc
    21241 ccggtatatt cattggggtc tggctctagg attgtcatgt actataatgg aggtggtgac
    21301 aattatattg gttctggtac tagattagct atggcgccac aatttggaaa tcatgtaaga
    21361 attcatactt caggttcttg gagtccagat tcttattaac ttacttgtca gagtaagcct
    21421 taaagatggt tgattgtggg tgtagcatga aaaaagaatg ctacacccta tttttattat
    21481 aaggaggagt aaggatggaa tttttcattt gtaatcttgt acgagtcgtt caatcacctc
    21541 ggttttatat gtctttattt ttgacccttc tttgcatgag tttaggaaat ttccttgctt
    21601 tcaatggtat ttataaaatt gaaggtttat cgattttttt tgccgcttct tctattcgag
    21661 gattttcacc gattagccta gtagctgcac ttatctgtac actgccctat tctagtcaga
    21721 taatagagga tgctgagagt cattttctaa cagcacaatt gtgccgaatt tctaaaaaga
    21781 ggtatctagc tattgtaggt agtactgcaa ttatttcttc ttttctagtc ttttttctcc
    21841 cctatttatt attattagga attaatcttt tagtgactcc ttatcaggaa atttatattg
    21901 gagattatag tggtgcctta aaagaattat ttgattctaa tcagtttctc tatagtcttg
    21961 tgacgactct ctggtatgga gtttggggcg ctgtgttctc tatttttgga ctagctagtg
    22021 ctttgctagt gaagaaaaaa ttaggagcta ttttcatccc agttgcctat atgatggttg
    22081 gtggtatttt atgggctatt ttagggctat cttacttaga acctgtgaca acgctagctt
    22141 tgggatatca gaaagatatc agtctttcct tagttagtgg gcatcttgct tttattttat
    22201 ttgttagttg tttggttgtt tatggtacat tttttctaca ttcagaggac tatgtataat
    22261 gaaacaattt gttcaatttt ataaaaaaga tttcttagca gtattggttt attttatatt
    22321 actgctatcc tgtgttttat ctagtacagt atatttattg cgctgtcgcc aatattcaat
    22381 ccatccaaat gtattagaat ggattttagt tttacttcaa gatatgacga ctggagtata
    22441 ttgctttccg ttcacatata tattgttctt tttttatttg atgaataact attttaatag
    22501 gttggagtgt cgcattcgtc tgaaatcaat taagcacttt accagtttta gtttcaaatt
    22561 agcagctctt agtacgggga tttggacggc gactttattt ttattgattt ttctaattgc
    22621 atttagtaat ggttttagct tctctttgga gataaaggag gttgattttt taagagaatt
    22681 ttatggtata agtattgcaa acaatgctag tttctttata ggattttttt tctcttatat
    22741 agcatactat ttctttttat ccttacttac tattagcagt ttttcttggt ttaaaaaatc
    22801 aaacatgagc ttagtatttc tgtttacttt tttatttgta gaatccttat tctggattta
    22861 tcagttgaac aatgggataa ttggattatt gccaattttt cagtatatgg taaattccaa
    22921 tccgtatgca ttgatttatt ggcttacatt actatctatc ataattccat tgactgtatt
    22981 ttctgttcat agaaactgga ggagagtgta aaagttggaa atgggaaagt taagtagtca
    23041 catgtggagg ttgaatcaga taatctatac caagtacttt tggggttatg ttcttttttg
    23101 gatattgatt tgtttaggat tatggtattg gttagaagga aatgatagac ttgttataga
    23161 aattttaaaa gggcctaatc tgagtcaaaa ctctttttta gtcttatcta tatggttgct
    23221 tcattggttt attattcata cattttttct agcagttgta tatcgtagaa gagcatccga
    23281 tttctttatg gaagtgattc gattttcttc tattaagctc tggattaggt atcagatttg
    23341 gacctgtttt ctttatggac tcattttaat catggtaaaa gttctagtga ttcaatttat
    23401 gttacagtta gcaaactggg atataggagt tttgtttata gttgattctt tgaatgcttg
    23461 tgtgctatcc ttgctttgct ttatgttata cgcactagga gcgaatgtac aaatgaactt
    23521 tgcttgcgtt agtttctttt tactcatgat tgtgtttggt ggtctatttg taggaaatcg
    23581 aactaattat ctattttata ttttgaatcg agggaatgga gatataggac gagatttatt
    23641 tcttcagttg ctatttttag tctttctttt taaaagcatt ttctatttca ctcgtcaaaa
    23701 gaggaggttt atagaatgaa tgaaataatt acattaaaaa atattgagtt gaaattaaaa
    23761 aaaacatgtg tttttcaaaa ccttaatttt agttgtaaac agggggaaat tataggaatt
    23821 actggtgcga atggctcagg taaaagtgta ttgtttaaat taatagctgg tttatatagt
    23881 ccgtcttatg gagaagtgtt aatcaatggg gaaaatattg ttcctgagag aaaaattcca
    23941 gctaatttgg gagctttgat tgaagaacct ggttttataa attattatag tggctttaag
    24001 aatttacaat atttggcaag catacgagga gtagttggta atcaggaaat caatgataca
    24061 ctgaaaatag ttggtctata tgagcaaaaa gaccagaaag ttaaaactta ttcgctaggt
    24121 atgaggaaaa agctagggat tgctcaagca attatggaga atccctctat tcttttacta
    24181 gatgaaccta tgaatgcctt ggataaatca agtgtagaaa atatgagaac attgtttaga
    24241 aagctctcta gtgaaaaagg gacaacaatt ttgattgcta gtcatagtga agaggatatt
    24301 cgtatcttat gtgataaagt atatgcaata gaagataaag tatgtacact gtgttcagat
    24361 tgaacatctt agatagactc aggatgttat aggtaaaatc ttaaaaaggg attggagctt
    24421 aaaatccagt cccttttaag agttaatggc caccttcaac aaccaggtca tctgctttag
    24481 cagcgtcacc gtaggttggg tgaagtgttt tttcataggc cttgtggaag aagttttcct
    24541 tgcctaattt ttcaatatct ttattgatga agtctagcaa ttcttggttg cctttttgaa
    24601 ctgctgccgc aatggtatcg ggatcaccga gggaagtaat tcctacttca aatcctttat
    24661 tttcaagtgc ccaagctaga acttccgtat tgtcagttga aaaggcatct ccacgtccgt
    24721 caagaagagc ttggtaagag tcactgtatt ggtcgtattt ttggagtttg atttctggat
    24781 gattcttttc aaaataagtc tcagcagtcg ttccttttgt gacaattaag gttttacctt
    24841 caagttgttt gacgtctgta atgagaccag tcttaggtga tacgacaccc agagaaactt
    24901 tcatatatgg aagggcaaaa tcaacttgtt tcttacgttc gtcagttact gtaaagttag
    24961 caagagtaat atctaccttg tttgaaatca agtattccgc acggttggca gcatcgattg
    25021 aaatgtattt aaccttgaca ccaaggtctt gagctagttg gttccctagt tcaatatcgt
    25081 agcgtagcct tggtaagaac catcattgtc aacgtagcca aacggttttt tatctccaaa
    25141 cacggcgatt cgcagttcac cgcttttttt gatttcatcg atagtgcgag ctttggcagt
    25201 tgtttttcca gaggatgaac cagcgtttcc acctgagcta caagcagtga taaagataag
    25261 ggcaaaggca agtgctaaaa cagttaagag tggtttgaat agtttcataa gaatctcctt
    25321 tatagatatg agccgaattg gctaaagtca aagacgttta aaaattcctg ggctcgtttg
    25381 gtttgcggat tggtaaagaa ggcttgagct gttccttctt cagcgatttt cccttggtcg
    25441 aggaagataa tccggtcagt aatggcttgg gcaaactgca tttcgtgggt tactaaaatc
    25501 atggtacggc cttcttgggc caaatcattg ataagttcca gcacctcacg caccatttct
    25561 ggatccagcg aagcagtcac ctcgtcaaaa aggatgattt ctggatgcat taggagggca
    25621 cgggcaattg caacacgttg cttctgtcca ccagataatt gacgggcaaa gcaaagctat
    25681 gttgtttatc cagcaaatcg acacgatcta gtaattgcaa agcttcttcc gttacttctt
    25741 tcttgtccct tccttgagtt ttgatagggc ctaggatgag gttttgtaag acatccagat
    25801 ggggaaagag ttcataactt tgaaagacca tgccaatctt ttggcgaact aggtgaaaat
    25861 cttttttatt ttcaacgata gactgaccat ccagaaaaat atctccacct tgaatacttt
    25921 ctaagccgtt gaggcaacga aggagggtac ttttcccaca accagatggc cccaggatga
    25981 caacaacttc ccttttttga tttctagaga aagtccttgg aggatgggat tgtctccgaa
    26041 ggattttttt agttccttga tttctaagat agtttcagac atttagtttc tccaatgttt
    26101 ttctaagtga gtggatagtt tggaaatagg gtagcaaact gcgaaatata agactagaat
    26161 ggttccataa atccaaaatg aagcagttgg gatggtcagg cgattgctat cgatgatttg
    26221 ttgtccaact ttggtcactt cc
//
LOCUS       DAVDWM010000028        24989 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVDWM010000028 DAVDWM010000000
VERSION     DAVDWM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.028"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            165..1289
                     /locus_tag="VUW49_001337"
     CDS             165..1289
                     /locus_tag="VUW49_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7981248.1"
                     /translation="MGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNFLSVIFSSIT
                     GDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKKVFFALLLLH
                     IVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQK
                     HLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLR
                     SDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMS
                     VLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFSLGFSTKGRN
                     RGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            1286..2038
                     /locus_tag="VUW49_001338"
     CDS             1286..2038
                     /locus_tag="VUW49_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981249.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            2090..2161
                     /locus_tag="VUW49_001339"
     tRNA            2090..2161
                     /locus_tag="VUW49_001339"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2123..2125,aa:Glu,seq:ttc)
     gene            2167..2240
                     /locus_tag="VUW49_001340"
     tRNA            2167..2240
                     /locus_tag="VUW49_001340"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2201..2203,aa:Asn,seq:gtt)
     gene            complement(2280..2822)
                     /locus_tag="VUW49_001341"
     CDS             complement(2280..2822)
                     /locus_tag="VUW49_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET7981250.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDKN"
     gene            3001..3768
                     /locus_tag="VUW49_001342"
     CDS             3001..3768
                     /locus_tag="VUW49_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET7981251.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            3772..4191
                     /locus_tag="VUW49_001343"
                     /pseudo
     CDS             3772..4191
                     /locus_tag="VUW49_001343"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4219..4533
                     /locus_tag="VUW49_001344"
     CDS             4219..4533
                     /locus_tag="VUW49_001344"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET7981252.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(4634..7186)
                     /locus_tag="VUW49_001345"
     CDS             complement(4634..7186)
                     /locus_tag="VUW49_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET7981253.1"
                     /translation="MKSFLKTYRTYFISLIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDILAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7248..8870)
                     /locus_tag="VUW49_001346"
     CDS             complement(7248..8870)
                     /locus_tag="VUW49_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7981254.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            9076..10101
                     /gene="trpS"
                     /locus_tag="VUW49_001347"
     CDS             9076..10101
                     /gene="trpS"
                     /locus_tag="VUW49_001347"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7981255.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            10253..11731
                     /gene="guaB"
                     /locus_tag="VUW49_001348"
     CDS             10253..11731
                     /gene="guaB"
                     /locus_tag="VUW49_001348"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7981256.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(11782..12879)
                     /gene="recF"
                     /locus_tag="VUW49_001349"
     CDS             complement(11782..12879)
                     /gene="recF"
                     /locus_tag="VUW49_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET7981257.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(12882..13250)
                     /locus_tag="VUW49_001350"
     CDS             complement(12882..13250)
                     /locus_tag="VUW49_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET7981258.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            13409..14659
                     /locus_tag="VUW49_001351"
     CDS             13409..14659
                     /locus_tag="VUW49_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7981259.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            14656..15939
                     /locus_tag="VUW49_001352"
     CDS             14656..15939
                     /locus_tag="VUW49_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7981260.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            15981..16802
                     /locus_tag="VUW49_001353"
     CDS             15981..16802
                     /locus_tag="VUW49_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7981261.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            16813..17358
                     /gene="pgsA"
                     /locus_tag="VUW49_001354"
     CDS             16813..17358
                     /gene="pgsA"
                     /locus_tag="VUW49_001354"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7981262.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            17355..18182
                     /locus_tag="VUW49_001355"
     CDS             17355..18182
                     /locus_tag="VUW49_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET7981263.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            18167..19006
                     /locus_tag="VUW49_001356"
     CDS             18167..19006
                     /locus_tag="VUW49_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET7981264.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRIFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            18999..19793
                     /locus_tag="VUW49_001357"
     CDS             18999..19793
                     /locus_tag="VUW49_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET7981265.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            19852..20670
                     /gene="mreC"
                     /locus_tag="VUW49_001358"
     CDS             19852..20670
                     /gene="mreC"
                     /locus_tag="VUW49_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7981266.1"
                     /translation="MNRFKKSKYVIIVFATVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            20670..21164
                     /gene="mreD"
                     /locus_tag="VUW49_001359"
     CDS             20670..21164
                     /gene="mreD"
                     /locus_tag="VUW49_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7981267.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            21258..22436
                     /locus_tag="VUW49_001360"
     CDS             21258..22436
                     /locus_tag="VUW49_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET7981268.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            22660..23439
                     /gene="rpsB"
                     /locus_tag="VUW49_001361"
     CDS             22660..23439
                     /gene="rpsB"
                     /locus_tag="VUW49_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7981269.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRTRLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            23518..24558
                     /locus_tag="VUW49_001362"
     CDS             23518..24558
                     /locus_tag="VUW49_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET7981270.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            24804..>24989
                     /gene="tnpB"
                     /locus_tag="VUW49_001363"
     CDS             24804..>24989
                     /gene="tnpB"
                     /locus_tag="VUW49_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET7981271.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKALYWDG"
BASE COUNT         8005 a         3957 c         5063 g         7964 t
ORIGIN      
        1 ttttaattat tagtcacagt taccgtttaa tttgtaaagg tcgaataaat agaaaagaat
       61 tatacgtttt tggtgcttat acattactaa ctgaaatagt acttgaattt tccttttatc
      121 ttctatattt agataaaata gggattgaaa gatttttatt tcctttgggc ttatattcct
      181 attttcgatg gatgaaacag tatgagaggg atagaggact attcctaagt ttactactat
      241 ctcttttata tgagagcact cataactttc tgtccgtaat tttctcctct ataacaggag
      301 ataattttgt tttacaatat catttcccat tctttttcgt tgtgacggtg ttaacctatt
      361 ttgttacatt aaaaatcatt tactatttcc atttggaact agcctatttt gacaaggact
      421 acctttatcc tttcttgaaa aaagtatttt ttgctttact attgctacat attgtatctt
      481 tcgtttcaga tatggtaagt acgattaaac atttgaatag ttttggaagt attttgtcat
      541 ctattgtctt tatctctctc cttttgacct tctttgcaat gaattctcat aaagttcaaa
      601 tggagaaaga gattgctttg aagcagaaga aatttgaaca gaaacattta cagaattaca
      661 cagatgaaat tgttggtctg tataatgaaa tccgtggttt tcgacatgat tatgctggaa
      721 tgcttgtcag catgcagatg gcaattgaca gtggtaattt acaggaaatt gacagaattt
      781 acaatgaagt tttagtcaaa gcaaatcata aattgcgttc agataagtac acttactttg
      841 atttgaacaa catagaagac tcagctttac gaagtttggt tgctcagtca attgtctatg
      901 ctcgaaataa tggtgtagag tttacactgg aagtaaaaga tacgattacc aagcttccaa
      961 ttgaactatt ggatttggtt cgtatcatga gcgttttatt gaataatgct gtcgaaggat
     1021 cggctgatag ctataaaaag cagatggaag tagcagttat taagatggaa actgaaacag
     1081 ttattgtgat tcagaattca tgtaaaatga cgatgactcc ttcaggagat ctattttcct
     1141 taggattctc cactaaggga agaaatcgcg gagtcggatt aaataatgtg aaagaactac
     1201 tagataagta caacaatatt attttagaaa cagagatgga aggcagtaca tttagacaaa
     1261 tcattagatt taagagggaa tttgaatgaa agttttaatt ttagaagatg ttattgaaca
     1321 tcaagtgaga ctagagagaa tattggatga aatttcgaaa gaatcgaata ttccaatatc
     1381 atacaagaca acgggaaaag tccgtgaatt tgaagaatac attgaaaatg atgaagtaaa
     1441 tcagctttat ttcctagata tcgatattca tggaattgag aaaaagggat ttgaagtggc
     1501 tcagctcatt cgtcattaca atccttacgc tattatcgtc tttatcacta gtcgatcaga
     1561 gtttgcgact ctaacctata aataccaggt atcagcccta gattttgttg ataaggatat
     1621 caatgatgag atgtttaaga agagaattga gcaaaatatc ttctacacga agagtatgtt
     1681 acttgaaaat gaagatgttg tagattattt cgactacaat tacaagggaa atgatttaaa
     1741 aattccttac catgatattt tgtatattga aacaacaggg gtatctcata aattgcgcat
     1801 tattggtaag aattttgcaa aagagtttta tggtaccatg acagatattc aggaaaagga
     1861 caaacatact cagcgatttt attctcctca caagtcattt ttggtaaata taggcaatat
     1921 cagagaaatt gatcgaaaaa acttagaaat tgttttctat gaagaccatc gttgtcctat
     1981 ttcaagatta aaaattagaa aattaaaaga tattttagag aaaaaatctc aaaagtgatt
     2041 gacaattagc aagaaattga tataatggtt atatctgcta cagatagaag gtccgttggt
     2101 caaggggtta agacaccgcc ttttcacggc ggtaacacgg gttcgaatcc cgtacggact
     2161 attttatccg ccatagctca gttggtagta gcgcatgact gttaatcatg atgtcgtagg
     2221 ttcgagtcct actggcggag tatagataaa agggaacaca gctgtgttcc ctttttgtat
     2281 caatttttat caccaagcat tttcataagg aagtctgtta tttcttgaga actttctttt
     2341 tttccatgtg caatccaagt ttggcagaca ccaaaaagtg catgagttag atagatgcta
     2401 ctatattcta attcggtggt atttagattc agttgcataa atcgcttttg taaatctgta
     2461 ctaagcatga tatgaagttt atttcgtaag aaattttgga tttctttagt cccattttca
     2521 gaaagaaggg cagccagaag tggttctgac tctagatatt caaaaacttc taaaatagcg
     2581 tctcttttgt gatgagcatg tttttgaaaa atatattcaa atgtatggaa tagcttgctt
     2641 tgatagtgct caatcatatc atacttatcc ttatagtgag tatagaagct ggaacgacta
     2701 attccggctt tttctactaa tttgacagta gaaattttat caaatggctg ttccatcagt
     2761 aattgtacca tagcattttc aatagttcgc tttgttttta agcgtttgtt actttcttgc
     2821 atatttcctc cttgtaaaca aattagacta tatgtctaaa aatagatttt ttatcttgta
     2881 atttagattt tttaatgtat aatctattat atcaaaattt tagacaatat gtttaaaaaa
     2941 ggagaaacta agtttaaaga atggaaagca atttaaaaaa aaccaacctt tattattgtc
     3001 atgatcggga tttctcttat tccagatctg tacaatatca tatttttgtc atcaatgtgg
     3061 gatccatatg ggcaattgtc tgacttacct gtggcagttg taaataatga taaagaggct
     3121 tcctataatg gtaatactat ggcaatagga aaagacatgg tgtccaattt aaaagaaaat
     3181 aaaaccttgg attttcattt tgtagatgaa gaggaaggaa agaagggatt ggaagatggc
     3241 gattactata tggtagtgac tttaccaagt gatttatctg aaaaaacaac tacattatcc
     3301 aatattcaat cgacagcagc ttatcaatca ttgacaagtg agcaacaaac tgagataagt
     3361 gattctgtat ctcaaaattc aactgatagt attcaatcgg ctcagtcaat tgtagcttta
     3421 gtacaagatt tacagggaag tttagaaaac ttacaaaatc aatcttctaa tctttcgact
     3481 ttaaaaaatc aatctaatca agtatcacct attacttcta cttctttgat aggattgtca
     3541 agtggattaa cagagataca aggagatgtt actagcaaat tagttcctgc cagtcagtcg
     3601 attgcatcag gtgtaaacgc atatactaca ggtgttgata aagtttctca gggcgcaagt
     3661 caactaagtg aaaaaaatgc caccttgaca ggtagtttgg ataaactagt ttcaggctca
     3721 aacaccttga cacaaaaatc ttctagattg acagcaggag ttggttaatt acaatcagga
     3781 tctgggcaat tagcagacaa atccagtcag ttactttcag gtgcttctcc attagagaat
     3841 agagctaata aattggcaga tggatctagg aaactagcag aaggtggaac aaagttaact
     3901 tctggattgg aagatttaca gacaggactt gcttctttag gacaaggact aggtaatgct
     3961 agtgatcaac tcaaatcagt atcaacagaa tctaaaaatg cagagatttt gtcaaatcca
     4021 ctcaatcttt caaaaacaga caatgatcaa gttcctgtaa atggaatcgc aatagctcct
     4081 tatatgatat cagttgctct tttttttgca gcaatatcaa caaatatgat atttgcgaaa
     4141 ttgccttcag gacgtcatcc agagagccgt tgggcttggt tgaaatcttg agctgaaata
     4201 aatggtatta tagctgtttt ggcaggaatt ttggtatatg gaggagttca gcttattggt
     4261 ttaactgcta atcatgagat gagaatattt attctcatca tcctaacaag tttagtattc
     4321 atgtctatgg tgaccacttt agcaacgtgg aatagccgta taggagcttt tttctcactt
     4381 attttgcttt tactacagtt agcatcaagt gcaggtactt atccacttgc tttgacaaat
     4441 gatttcttta gatctattaa tccctggtta ccaatgagct attcagtttc gggattacga
     4501 caaacaatct ctatcaacaa gtcattttcc tagctgtcat actagctcta tttactggtt
     4561 taggtatgct agcctatcaa cataagaaaa tggaagaaga ttaaaaaaat cgaccgatta
     4621 actggtcgat tttttatgcc ttagatgact ttcgtctgtg attatagatt ccaaatagta
     4681 agagagaagt aaaggaacag attgctccag taataaaacc attgggaatg aaggaaagtg
     4741 taatagttcc tttccccttg ggaatgtcaa ctttcataaa tccagtttga gcttgtttaa
     4801 tttctatttt cttaccatct tggtaggcag accaaccttt gtcataagga atggtgaaga
     4861 aaatagatgt atcttgttgg acatcatatg tagcaaaaac cttgttttta gaagttgata
     4921 ctgtgacagg ttgttcttta attttttgaa ttgcctcggt gaaagttttg gtatctaaac
     4981 gatagaaggt aggagattca aatgatactt gtgaatttcc agggaaacta acattgatat
     5041 tgaaagtttt tttctcttta gtatatccta gattaaagaa ggagaagaca ttatcagttg
     5101 taaaagtctt tttttcacca tttacaagga tgtcaacctt cttttgttta tcgttagaaa
     5161 agtgaaggtt tatgaaagag agataaactt ggctgttttc tggaacttca atttgatact
     5221 ggattgctgc atcttcattt gaagaacttg tgacactaat caaatcatta gtattttcta
     5281 ttttttctgt tttttcataa ggtattggag aaaaataatc aaaattgacg ttagcaagtt
     5341 gatttaaaaa tgaggcctga ttatccaagg tatgttcatt gaacttgaca tcattgtaaa
     5401 cagattgact agcaactgca atcggaagag agtattgatt ttcatatagg gtaagattat
     5461 ctttttgata gatatcttta aagccatact tatcaatagg actgtctgag atattgtact
     5521 ggataccaaa taaactatca gccaaaatac tattatttgc atatcggaga ttgagattag
     5581 tcccagagga tttaaaacca agtttatcta aagtagagct tgatgaacga tttcgaacag
     5641 atgaaaattg agagattcca ttgtagttga atttcatact gtcatttcct gtctgagttt
     5701 gtagtttttc agtacgagta aattgatttc caatatatgt tgagaaagat tccatagcta
     5761 ggatatctcg actataagca cttcgagaag caaatcccca ttccttagca attccgtcca
     5821 tttgagatga agcatttaaa ctcatttcaa ccagtataaa taaagagatt agaatggcaa
     5881 atagattcac agatataaac tttttgataa ctgcaaggag taaaagagaa tagacaacca
     5941 aaaattcaag agtaagcaga atattcaaat ctgttaaaaa agaataatgc gattttagat
     6001 agatggtagc taaaaatcct gctactacaa gaaaaagcga aactaaaaaa ttccagactt
     6061 taagttcttt cagacgcttt aagacttctg ctgctgtgta aattaacaag gtagagaaaa
     6121 tccaagcata gcgatgtaaa aacatgtttg gagtatgcat gccttgccaa aataagtcaa
     6181 gagcttctat gtaaaagctt gcaattagaa atgcaaagaa tattacatat atgagtttca
     6241 cgtgaaactt aatagatttc agcgtaaaaa ataaaatggt caaaataaag ggaaatagtc
     6301 caacaaaaat cattgggatg gccccatact ttgttgtgtc aaaggaacca atgaattgct
     6361 tagcaaagag atcaagatac cagctacttt cagtttgaaa ctttgtaact tcagtcaatt
     6421 tttccccatg tgtctgtaaa tcaaatagag tgggaagagt cataatcaaa ctagccatac
     6481 cagctaaaaa ggagataact atgaaatcaa gaacagatga ttttcgagtc ttaaagtccc
     6541 acgaaatttg acagagatac cagaaaataa gaaacaatac tgtcatatat ccaaaataat
     6601 aattttgaat aaataagatt gacagacttg taaagtacaa taggagtttc ttttcagtta
     6661 tcagtagatg taaaccagtt ataattaaag gaatcaagat aaaaacatct agccaggttt
     6721 ttatctctaa ttgactgaca gtgaaactca tcagagcata ggaagtagat aaggctagtt
     6781 ttaaagtctg agggatagat tgaaacaatt tattcaaact aaaaaaggtt gacagaccaa
     6841 tcaatccaaa ttttaagaga gtggtcagat agacagcatc tggcatgttc gttagatcaa
     6901 aaaagtaaac tagaggcgcg agaaaactac ccaagtaata actagatagg gcatagaagt
     6961 ttagccctag accacttgta aaggtgtaaa acagactact atttccatgt aggatatttc
     7021 gtaaggctac atcaaaaata acgtattgat gaaagccatc tcctaataga ggagagttgt
     7081 cgctattcca gtagatacct tgagatagat atactccaga cataatcact acaggaatga
     7141 tgagagaaat aaaataggtt cgatatgttt ttaaaaatga tttcatgtta cctcgtagaa
     7201 tgatagaaaa ctcagttggt taacccaact gagttttgaa gttttattta gtctttccaa
     7261 agttctttaa cttttgcttg tacttctgca ttttctagga attcatcgta ggtttcatcg
     7321 atacggtcaa tgacgccatt tttagataag acaatgatat ggttagccaa agtttgaata
     7381 aattcgtggt catggctagc aaagatgatt gattctttaa agtttttcaa tccatcattc
     7441 aagcttgaga tagattccaa gtccaagtga tttgttggat catcaagtac aaggacattt
     7501 gattttaaga gcatgagttt tgaaagcatg acacgaactt tttctccccc tgacaagaca
     7561 tttacaggtt tgttaacttc atctccagag aagagcatac ggccgaggaa gccacgtagg
     7621 aaagtattgt catcttcttc tttacttgcg aattgacgca accagtcaag aattgattct
     7681 cctcctgcaa aatcagctga gttatctttt ggtaggtaag attgactagt tgtaactccc
     7741 cacttgacag ttccttcata gtcaatatct cccatgattg cacgaattaa tgcagtcgtt
     7801 tgaatatcat tttgtccaat aagtgctgtc ttatcatctg gacgcaagat gaaactaata
     7861 ttatccaaga tagtttcacc atcaatcttt acagttagat tttctactgt caagagatca
     7921 ttaccaatct cacgttccgc tttaaagttg ataaatggat atttacgact agatggtaca
     7981 atctcttcta gctcaatctt atcaagcatt ttcttacgtg atgttgcctg ccttgactta
     8041 gaagcattgg cagagaaacg agcaacgaat tcttgcaatt gtttaatttt ttcttctgct
     8101 ttagcattac ggtctgctag caatttagca gcaagctcag aagattcctt ccagaagtcg
     8161 tagtttccga catagagttt gatttttcca aagtcaaggt cggccatgtg agtacaaact
     8221 ttgtttaaga agtgacggtc gtgggatact acgataactg tgttatcaaa gtcaatcaag
     8281 aagtcttcta accaagtaat cgattggata tccaaaccgt tagtaggctc gtccaagaga
     8341 agaacatctg gtttaccaaa aagtgctttg gcgaggagaa cctttacttt ttcaccgttg
     8401 gccaattcgc tcatgttttg gtagtgtaat tcttctggaa tgtttaggtt ttgaagtagt
     8461 tgagaggctt cactctctgc ttcccaacct ccaagttcgg caaactctcc ttcgagttcg
     8521 gcagcacgaa ccccgtcctc gtctgagaaa tcttccttca tgtagatagc atctttctct
     8581 ttcatgatgc tataaagttt ttcatttccc atgataacga catcaatggc acgttcatct
     8641 tcgtagtcaa agtgattttg acgaagaaca gagagacgtt catctggacc aagagagatg
     8701 tgaccagtag taggttcgat atctccagct aaaattttta aaaaggttga ttttccggca
     8761 ccattagcac cgattaatcc gtaagtattt ccttctgtaa atttgatatt gacatcatca
     8821 aaaagtttgc gatcactaaa acgtagtgaa acatcagata ctgtaagcaa tgtttttctc
     8881 ctatatgtgt aatatattta ttctactaga aaatacagaa atattcaaat ttttatttgt
     8941 caattttgtg taaattatat ttacagtatc ctttacacaa atctgtaaaa agcaaggctg
     9001 atttattttg ataaattacg gttatttcat taaaaaaatg ctataattga aaggactata
     9061 tcgaaggaga acaaaatgac taaacccatt attttaacag gagaccgtcc aacaggaaaa
     9121 ttgcatattg gacattatgt tggaagtctc aaaaatcgag tattattaca ggaagaggat
     9181 aagtatgata tgtttgtgtt cttggctgac caacaagcct tgacagatca tgccaaagat
     9241 cctcaaacca ttgtagagtc tatcggaaat gtggctttgg attatcttgc agttggattg
     9301 gatccaaata agtcaactat ttttattcaa agccagattc cagagttggc tgagttgtct
     9361 atgtattata tgaatctagt ttcgttagca cgtttggagc gaaatccaac agtcaagaca
     9421 gagatttctc agaaaggatt tggagaaagc attccgacag gattcttggt ctatccaatc
     9481 gctcaagcag ctgatatcac agctttcaag gctaattatg ttcctgttgg gacagatcag
     9541 aaaccaatga ttgagcaaac tcgtgaaatt gttcgttctt ttaacaatgc atataactgt
     9601 gatgtcttgg tagagccgga aggtatttat ccagaaaatg agagagcagg gcgtttgcct
     9661 ggtttagatg gaaatgctaa aatgtctaaa tcactaaata atggtattta tttagctgat
     9721 gatgcggata ctttgcgtaa aaaagtaatg agtatgtata cagatccaga tcatatccgc
     9781 gttgaggatc caggtaagat tgagggaaat atggttttcc attatctaga tgtttttggt
     9841 cgtccagaag atgctcaaga aattgctgat atgaaagaac gttatcaacg aggtggtctt
     9901 ggtgatgtga agaccaagcg ttatctactt gaaatattag aacgtgaact gggtcctatt
     9961 cgtgagcgcc gtattgaatt tgctaaggat atgggagaag tttataatat gattcaaaaa
    10021 ggtagtgaaa gagcgcgtga agttgcgggt caaaccctat ctgaggtaaa aggggcaatg
    10081 ggacttcatt actttaacta agtttatttt acaagaaact atcttttcta tctcgaagaa
    10141 aagatagttt tttatttacc cctgtaacat ttgacaaatt tttatagaat ggtatgatag
    10201 atacaatata aaaagagtca agctcaaaaa gaaagaaaag aggaaacttc gaatgtctaa
    10261 ttgggacact aaatttttga aaaaaggttt tacctttgat gatgtattgc ttattccagc
    10321 tgaaagtcat gtgttgccta acgatgcaga tttaacaact aaattggcag ataatttgac
    10381 tttaaatatc ccaattatta ccgctgccat ggacacagtt acagagagtc aaatggccat
    10441 tgctattgct cgtgcaggcg gtctcggagt tatccataaa aacatgtcaa ttgctcaaca
    10501 agcagacgag gttcgtaagg taaaacgttc tgaaaatgga gttattattg atccgttctt
    10561 cttgacgcct gaacatacaa ttgctgaagc agatgagctt atgggtcgtt accgcatcag
    10621 tggtgttcca gttgttgaaa cacttgaaaa tcgtaaattg gttggtattt tgacaaaccg
    10681 agatcttcgt tttatttcag attataatca accaatttca aaccatatga ctagtgaaaa
    10741 tcttgttact gctcctgtgg gtacggatct tgcaacggct gagagtattc ttcaagagca
    10801 tcgtattgaa aaacttccgt tggtcgatga agaaggcagt ctttctggtt tgatcactat
    10861 caaagatatt gaaaaagtta ttgagtttcc aaatgcggct aaagatgagt ttggtcgtct
    10921 tctagttgca ggtgcagtag gtgttacttc agatacattt gaacgtgcag aggctctttt
    10981 tgaggcagga gcggatgcga ttgttattga tactgcacat ggtcattctg caggtgtctt
    11041 gcgtaaaatt gccgagattc gtgctcattt cccagatcgg actttgattg ctggaaatat
    11101 tgctactgct gaaggtgcac gtgcccttta tgaagcgggt gtagacgttg ttaaggttgg
    11161 tattggacca ggttctatct gtactactcg tgtgattgct ggtgttggtg ttccgcaagt
    11221 aacagctatc tacgatgctg cagctgttgc gcgcgaatat ggtaaaacga ttattgctga
    11281 cggtgggatc aagtattctg gagatattgt aaaagcactt gctgcaggtg gaaatgctgt
    11341 tatgcttgga tctatgtttg ctggaactga tgaagctcca ggcgaaactg aaatcttcca
    11401 aggacgtaaa ttcaagactt accgtggtat gggatcaatt gctgctatga agaaaggttc
    11461 aagcgaccgt tatttccaag gttctgtcaa tgaagcaaac aagcttgttc cagaaggaat
    11521 tgaaggtcgt gttgcttata aaggagcggc agctgatatt gttttccaaa tgattggtgg
    11581 tattcgctct ggtatgggtt actgtggtgc agctaacctt aaagaactac acgataatgc
    11641 tcaatttatt gaaatgtctg gtgctggttt gaaagaaagc catcctcatg atgtgcaaat
    11701 tactaatgag gcaccaaatt attctatgta aaaaacaatg aaaagaactc cagtgaaaac
    11761 aggagttctt ttacaatgtt gtcaatttcc atttacagca gctttaccat cctgaatagt
    11821 gaagatactt agattttctg gcagattttg aagatggtct aagcttgttg ttgtgataaa
    11881 ggtttggatt gattgagaaa tcgtttctaa taattttaac tgtctggtgt tgtcaagttc
    11941 actcatcaca tcgtcaagca gtaatatagg agattctgtg gtaatgcttt ccattaattc
    12001 gatttctgct aattttatcg agaggacgag actacgatgt tgaccttggc ttccgaaact
    12061 agcatccatc ccatttatat aaaaagaaat gtcatctcga tgaggaccga caccagtatt
    12121 ctttttaaat aaatctctgg atctactttt ttctaaagca attttgaaag attcggataa
    12181 gttttgtttg tcagttatat tgacagaaga ttgataggat attgacaact cttcgatctg
    12241 attagagagt tcaaaatgtt tcttacgccc aaatgattct agttttttta tgaaatctaa
    12301 gcggtgattc attacacgac atccataatc aactagctga tcatctaaca cagaaaggaa
    12361 tgtttcatct attttttgag ctgattttag gtaagtgttt ctttgcttta ggatgtggtt
    12421 ataattggtt aagtcagata aatagattgg cttaatttgc ccaagttcca tatcaatgaa
    12481 ttttcgtcga atcgaaggtg ctcctttaat tagttgtaaa tcttcaggag caaataagac
    12541 aacattcatg tgtcctacat aatctgaaag gcgtgcctgt tttaagtgat taacttttgt
    12601 tacacggcct ttttgtgtta gttcgatttc taggggaatg gatccagttt ttttctgaac
    12661 gagacctgaa agatgaagtt gttcctcatc aaaatgaatg agatttttat ctgttcgagt
    12721 tcgatgacta cgcgttaagg ctaaaaaata gatagcctct aacatgtttg tttttccttg
    12781 tgcattacgt cccaaaaaga cattcaattt aggattaaag tctattttcg tttctttgta
    12841 attacgaaaa gtcttgagag atagatgttg tagccacatg attatctacc agggaatcgt
    12901 ggagcttgtt ggcttttagg tgatgaagca ggtttcgatt tgtctttttt aactccctta
    12961 ttcatctttt tgacaagttt agcgatccgt tctttttcaa ctttatcagc ttggtattca
    13021 tcttgttctt cagaagtagg ttgtgtcaac aagatgtcaa tgttcatgtc agggatgtca
    13081 actttatcgc caatacgaag tttttttcca cgacaactct ctaattccct attaaagtaa
    13141 acagaatgtt cagagagaaa tgatttaata gctcctccgc tatgtgtaat tccaagttct
    13201 ttgagtagtg cttggagggt aataaattct tcaaataatt tgtattccat aattcacctc
    13261 aatctttggt attataccat attttcctaa aaatagtgag agagacagct agaaatgtaa
    13321 gcgattttta tttcaaaagt gttacaggga acaagaaaat ttcaggtttt cgtgatataa
    13381 tagaagtctg tatataagga ggtaaatcat ggagttagtg catggaattt caacacattt
    13441 tatccaatca aaaaagttta aaacaaacaa aattaccgtg cgttttaccg ctccattatc
    13501 ccttgatacg attgcaggtc acatgttgag tgcaagtatg ttagagactg ctaatcagat
    13561 gtaccccact tctcaagatt tgaggagaca cttggccagt ctatacggta cagatatgtc
    13621 aaccaattgt ttcagaagag ggcaaagcca cattatagaa ttgacattta cctatgttcg
    13681 tgatgagttt ttaagtagga aaaacgtgct aacctctcag attttggaac ttgtaaaaga
    13741 aactcttttt tcacccgcag tagttgataa tgggtttgat ccggccttat ttgaaattga
    13801 gaaaaaacaa ttgctagcaa gtttagcagc tgatatggat gattcttttt attttgcaca
    13861 taaagaattg gataaattgt tttttcatga tgaacgtctt caattggaat atagtgattt
    13921 acgaaatcgt attttagctg aaactccaca aagttcttat tcttgtttcc aagaattttt
    13981 agccaatgat cgaatagatt tctttttcct aggtgatttt aatgaggttg aaattcaaaa
    14041 tgtattagaa tcatttggct ttaaaggtcg aaaaggagat gtgaaggttc agtattgtca
    14101 accttattct aatatccttc aggaaggtat ggttcggaaa aatgtgggac aatccatttt
    14161 ggaattaggt tatcattacc gttctaaata tggtgatgaa caacatttac ccatgattgt
    14221 aatgaatggt ttacttggtg gatttgctca ctctaagctc tttacaaatg tccgtgaaaa
    14281 tgctggatta gcttatacca tttcaagtga gcttgattta tttagtggat tcttgaggat
    14341 gtatgctggt atcaatcgag aaaatcgtaa ccaggctcgt aaaatgatga ataatcaact
    14401 gcttgattta aaaaaaggtt attttacaga gtttgagtta aatcagacca aggaaatgat
    14461 tcgttggtcg ttgttacttt ctcaagataa tcaatcttca ttgattgaac gtgcttatca
    14521 aaatgcctta tttggaaaat cttcagcaga ctttaaaggt tggattgcaa aactcgagca
    14581 agttgacaaa gatgctattt gtagagcagc taataatgtg aaactacagg cgatttactt
    14641 tatggaagga atagaatgac aaaggttgtt tttgaaaaaa aatactatcc agctgtaaaa
    14701 gaaatgattt atcgaactcg tttggccaac ggattgacag ttgctctttt gcctaaaaag
    14761 gaatttaaag aggtttacgg gagtgtcact gtacagtttg gttcggtaga tacgtttgtc
    14821 acagaagttg acggagatgt aaaacaatat cctgcaggaa ttgctcattt tcttgaacat
    14881 aaattatttg agagagaaga ttctagtgat ttgatgtcgg cttttacgag tctaggtgca
    14941 gatagtaatg cctttacaag ctttacaaaa acaaactatc ttttttcagc aacggattat
    15001 tttttagaaa atttatattt acttgatgaa ttggtaacat cagcacactt tactgaagct
    15061 tccattctga cagagcagga tattattcag caagaacgag aaatgtacca agatgatcca
    15121 gattcgtgtt tattcttttc aactttagcg aatttgtatc ctggtacacc tttagcaact
    15181 gatatagttg gaagtgagga gtccatttcc caaatcaatc taactaattt gcaagaaaat
    15241 tttacaaagt tttacaaacc tgtaaacatg tctctgtttt tagttggtaa ttttgatgtg
    15301 gagcgagtac aggactattt tgaaagcaaa gaactgaaag attcagattt tcaggaagta
    15361 gcaagagaaa agttgttttt acagcctgta aagccaacag atagtatgag aatggaagta
    15421 tcttctccca aactagcgat tggagttaga ggtaagcgag aagtttctga agcggattgc
    15481 tatcgacatc atattttatt aaaattattg tttgcaatga tgtttggttg gacttcggat
    15541 cgttttcaaa aatgttatga atcaggtaaa attgatgcgt ccttatctct ggaagttgaa
    15601 ataacaagtc gctttcattt tgtcatgttg acaatggata cgaaagagcc agttgctttg
    15661 tctcatcaat ttaggaaggc tattcgtaat tttacaaagg atttagatat tacagaggaa
    15721 catttagata ttatcaaaag agagatgttt ggcgaatttt tcagtagcat gaactctctt
    15781 gaatttattg caacgcaata tgatgctttt gaaaatggtg agacaatttt tgatttgccg
    15841 aaaattttac aggaaattac tttagaggat gtccttgatg ctggacatca tttaatagat
    15901 gatggtgaca tagttgattt tacaatattc ccatcgtagt aacctatcat aatagacact
    15961 agaaagaagg gatgacaagt atgagaaaaa aaacaattgg agaggtttta cgattagcta
    16021 gaatcaatca gggattgagt ttagatgaat tgcagaaaaa gacagaaatc cagttagata
    16081 tgttggaagc aatggaagca gacgatttcg atcaacttcc aagtcctttt tacacgcgtt
    16141 ctttcttgaa aaaatatgca tgggctgttg agttagatga ccaaattgtt ttggatgctt
    16201 atgattctgg gagtatgatt acttatgagg aagtagatgt tgatgaagat gagttgacag
    16261 gtcgtagacg ttcaagtaag aaaaagaaga aaaaaacatc atttttacct ttattttatt
    16321 ttatcctttt tgctttatcg attttaattt ttgtgactta ttatgtttgg aactatattc
    16381 aaactcaacc agaggagcct tctctttcta attacagtgt ggttcaatca acaagttcaa
    16441 ctagctctgt tccccactcc tcaagtagta gttcttctag tatagaatca gctataagtg
    16501 tatcaggcga aggaaatcat gtagaaatcg cttataagac aagtaaggaa acagttaaat
    16561 tgcaattggc agtttcagat gttacaagtt gggtcagtgt ttcagaaagc gaacttgagg
    16621 gcggtgtaac cttatcgcca aagaagaaaa gtgcagaagc aacagttgca actaaaagtc
    16681 ctgtaacaat tacgttaggt gttgtaaaag gtgttgattt gacagtagat aatcagactg
    16741 ttgatttatc gaaattaaca gctcagactg gacaaatcac tgtaaccttt actaaaaatt
    16801 aaggaaaaac gaatgaaaaa agaacaaatt cccaatctct taacaatagg tcgaattctc
    16861 tttataccta tttttatctt tattttaacg ataggaaatt cgatagagag tcatatagtt
    16921 gcagctatta tctttgctgt tgccagtatt accgactatt tagatggata tttagctcgt
    16981 aaatggaatg tggtcagtaa ttttggtaaa tttgcagatc ctatggcgga taagttacta
    17041 gttatgtcgg cttttattat gttgattgag ttaggtatgg ctccggcttg gattgttgca
    17101 gtgattatct gtcgtgagtt agctgtgaca ggtttaaggc ttttattggt tgaaactggt
    17161 ggaacaattt tagcagcagc aatgcctgga aaaattaaaa cttttagtca gatgtttgcc
    17221 attattttct tgctattaca ttggactttg cttggtcaag ttctacttta tgtagcctta
    17281 tttttcacta tctactctgg ctatgactat ttcaagggta gtgcctatgt atttaaaggg
    17341 acatttggtt cgaaatgaaa tcaataattg atgtaaaaaa tctttctttt cgctataaag
    17401 aaaatcagaa ctactacgat gtgaaggata ttacgtttca cgtgaaacgt ggagaatggc
    17461 tttcgattgt aggacataat ggtagtggta aatcaacgac ggttcgatta attgatggct
    17521 tactggaagc agaatccgga gagattgtaa ttgatggcca acgtttgact gaggaaaatg
    17581 tttggaatat acgtcgtcaa atcggtatgg tttttcaaaa tccagacaat caatttgttg
    17641 gagcgactgt tgaagatgat gttgcttttg gtttggaaaa tcagggactt tctcgtcaag
    17701 aaatgaaaaa gagagtagaa gaagctctgg ctttagttgg catgttggac tttaaaaaga
    17761 gagagccagc gcgtctatca ggtggccaaa agcaacgtgt ggccattgca ggtgttgtag
    17821 ccctaagacc agctatttta atcttagatg aagcaacgag tatgttggat cctgaggggc
    17881 gtagagaact tattgggaca gtaaaaggaa ttcgaaaaga ctatgatatg acggtcattt
    17941 ctattaccca tgatttggaa gaagtcgcca tgagtgatcg cgtattggtc atgaaaaaag
    18001 gggaaattga atcaactagt agtccaaggg agcttttctc tcgaaatgat ttagatcaaa
    18061 ttggattaga cgatcctttt gctaatcaat taaaaaaatc tttgagccag aatggctatg
    18121 atttacctga aaattatttg acagaaagtg agctagagga taagctatgg gaattgctct
    18181 agaaaatgtg aattttacat atcaagaagg tactccctta gcttcagcag ctttgtcgga
    18241 tgtttctttg acgattgaag atggctctta tacagcttta attgggcaca caggtagtgg
    18301 taaatcaact attttacaac tcttaaatgg tttattggtg ccaagtcaag ggagtgtgag
    18361 gatttttgat accttaatca cctcgacttc taaaaataaa gatattcgtc aaattagaaa
    18421 acaggttggc ttggtatttc agtttgctga aaatcagatt tttgaagaaa cggttttgaa
    18481 ggacgttgct tttggaccgc aaaattttgg agtttctgaa gaagatgctg tgaagactgc
    18541 gcgtgagaaa ctggctctgg ttggaattga tgaatcactt tttgatcgta gtccgtttga
    18601 gctgtcaggg ggacaaatga gacgtgttgc cattgcaggc atacttgcca tggagccatc
    18661 tatattagtc ttagatgagc caacagctgg tctagatcct ctagggagaa aagagttgat
    18721 gaccctgttc aaaaaactcc accagtcagg gatgaccatc gtcttggtaa cgcatttgat
    18781 ggatgatgtt gctgaatatg ctaatcaagt ctatgtaatg gaaaagggac gtttagtaaa
    18841 ggggggcaaa ccaagtgatg tctttcaaga cgttgttttt atggaagaag ttcagttggg
    18901 agtacctaaa attacggcct tttgtaaacg attggctgat agaggcgtgt catttaaacg
    18961 attaccgatt aagatagagg agttcaagga gtcgctaaat ggatagtatg attttggggc
    19021 gttatatccc aggggattcg attgttcacc gattggatcc acgtagcaaa ttgcttgcta
    19081 tgatgctact gattttgatt gtgttttggg ctaataaccc cttgacgaat ctgattcttt
    19141 ttatagcgac agggatattt attgccttgt caggagtatc tctttcattt tttattcagg
    19201 gcttgaagtc tatgtttttc ttgattgcct tcacaactat ttttcaacta tttttcattt
    19261 ctaatgggaa tgttttattt gagttttcgt ttgtgagaat cacggattat gctttgcaac
    19321 aagctgggat tattttttgt cgctttgtat tgattatttt cttttcaact ttgttaacct
    19381 taacaaccat gcctttaagt ttggcatcag ctgtcgaagc tttattagca cctttaaagc
    19441 gtgtgaaagt tccagttcat gaaattggat tgatgctgtc tatgagtttg cgttttgtcc
    19501 caaccttgat ggatgatacg actcggatta tgaatgcaca gaaagctcgt ggagtggatt
    19561 ttggagaagg aagcatcgtt caaaaagtaa aggcgatgat tcccattttg attcctcttt
    19621 ttgcgacaag tttaaaacgt gcagattcct tggctatcgc tatggaagcg cgtggctatc
    19681 agggtggaaa aggcagaagt caataccgac aattgaaatg gactctaaag gatacgctga
    19741 ccattcttgt tattctcgta cttggttgtt gtttattttt cttaaaatct tagtagcttt
    19801 caggaattga taaaaagtta ctgtaacagt ttttgataga aaggtaggga tatgaaccgt
    19861 tttaaaaaat caaaatatgt cattattgtt tttgccactg ttctgcttgt gtcagctctc
    19921 ttagcgacga cttattcaag tacaattgtg acaaaattag gagatggaat ctcattggtt
    19981 gatagagttg tacaaaaacc ttttcagtgg tttgattctg tcaaatcaga tttggctcat
    20041 ttgacacgaa catataatga aaatgaaagt ttgaagaaac agctttacca attagaagtt
    20101 aaatcaaatg aggtggaaag tttaaagaca gaaaatgaac aactgcgcca attgcttgat
    20161 atgaagtcta aattgcaagc cacaaagact ttagcagcag atgttattat gcgttctccg
    20221 gtatcttgga agcaggagtt gaccttagat gcaggcagat caaaaggtgc ttctgagaac
    20281 atgttagcta ttgcaaatgg tggcttgatt gggagtgttt caaaagtaga ggagaactct
    20341 actatagtca accttctgac aaatacggaa aatgctgata agatttctgt taaaattcaa
    20401 catggctcta ctacaattta tggaattatt attggctatg acaaggaaaa tgacgttctt
    20461 aaaattagcc aattaaatag taatagcgat attagtgcgg gagataaggt gactactggt
    20521 ggattaggaa actttaacgt tgctgatatt cctgttggtg aagtggttgc cacaacgcat
    20581 agtacagact atttgacacg agaagtaact gttaaattga gtgcagatac tcataatgta
    20641 gatgtgatag aattagtggg gaattcataa tgagacagtt gaagcgagtt ggagtatttt
    20701 tattgcttcc tttctttgtt ctaattgacg cccatattag ccagcttctg ggctcatttt
    20761 tcccccatgt acatttggct agtcattttc tttttctatt tctcttattt gagacgatag
    20821 aagtatcaga gtatctctac ctagtctatt gttttgttat aggcttggtt tatgatgttt
    20881 actttttcca tctaataggg attacaactc tcttatttat cttattggga gccttccttc
    20941 ataaattgaa tagtgttatt ttgttgaatc gttggacaag aatgctagct atgattgtgc
    21001 tgacattcct gtttgaaatg ggtagttatc ttttggcttt tatggtaggg ttgacagtag
    21061 atagcatgtc gatttttata gtctatagct tggtaccgac gatgatttta aattttttat
    21121 ggattactgt ttttcaattt atttttgaaa aatattatct ataagaacga catataaatg
    21181 taacaaaggc gtaatattta ttaggccttt ttttggtata ctagtattgt ctttaaaaga
    21241 aggagtatct acgtaatatg aagaaaaaaa tcttagcgtc acttttatta agtacagtaa
    21301 tggtttctca agtagctgtt ttaacaactg cgcatgcaga aacgactgat gacaaaattg
    21361 ctgctcaaga taataaaatt agtaacttaa cagcacaaca acaagaagcc caaaaacaag
    21421 ttgaccaaat tcaggagcaa gtatcagcta ttcaagctga gcagtctaac ttgcaagctg
    21481 aaaatgatag attacaagca gaatctaaga aactcgaggg tgagattaca gaactttcta
    21541 aaaacattgt ttctcgtaac caatcgttgg aaaaacaagc tcgtagtgct caaacaaatg
    21601 gagccgtaac tagctatatc aataccattg taaactcaaa atcaattaca gaagctattt
    21661 cacgtgttgc tgcaatgagt gaaatcgtat ctgcaaacaa caaaatgtta gaacaacaaa
    21721 aggcagataa aaaagctatt tctgaaaaac aagtagcaaa taatgatgct atcaatactg
    21781 taattgctaa tcaacaaaaa ttggctgatg atgctcaagc attgactacg aaacaggcag
    21841 aactaaaagc tgctgaatta agtcttgctg ctgagaaagc gacagctgaa ggggaaaaag
    21901 caagtctatt agagcaaaaa gcagcagctg aggcagaggc tcgtgcagct gcggtagcag
    21961 aagcagctta taaagaaaaa cgagctagcc aacaacaatc agtacttgct tcagcaaaca
    22021 ctaacttaac agctcaagtg caagcagtat ctgaatctgc agcagcacct gtccgtgcaa
    22081 aagttcgtcc aacatacagt acaaacgctt caagttatcc aattggagaa tgtacatggg
    22141 gagtaaaaac attggcacct tgggctggag actactgggg taatggagca cagtgggcta
    22201 caagtgcagc agcagcaggt ttccgtacag gttcaacacc tcaagttgga gcaattgcat
    22261 gttggaatga tggtggatat ggtcacgtag cggttgttac agctgttgaa tcaacaacac
    22321 gtatccaagt atcagaatca aattatgcag gtaatcgtac aattggaaat caccgtggat
    22381 ggttcaatcc aacaacaact tctgaaggtt ttgttacata tatttatgca gattaattta
    22441 cagagggact cgaatagagc cctcttttca ggttttaccg tgacaatccc tattaaaaat
    22501 tatatcaaaa tagcttgaaa atattggaaa agtatggtag aatgaaaatt gtcgtgtgaa
    22561 cgataatact cattcttgat gaattgtgaa gcagttgccc ttgggtcgtt ttgcgagttg
    22621 aagtcaagaa gaggaaaaaa acaaaaagga gaaatactca tggcagtaat ttcaatgaaa
    22681 caacttcttg aggctggtgt acactttggt caccaaactc gtcgctggaa tcctaagatg
    22741 gctaagtaca tctttactga acgtaacgga atccacgtta tcgacttgca acaaactgta
    22801 aaatacgctg accaagcata cgacttcatg cgtgatgcag cagctaacga tgcagttgta
    22861 ttgttcgttg gtactaagaa acaagcagct gatgcagttg ctgaagaagc agtacgttca
    22921 ggtcaatact tcatcaacca ccgttggttg ggtggaactc ttacaaactg gggaacaatc
    22981 caaaaacgta tcgctcgttt gaaagaaatt aaacgtatgg aagaagatgg aactttcgaa
    23041 gttcttccta agaaagaagt tgcacttctt aacaaacaac gtacgcgtct tgaaaaattc
    23101 ttgggcggta tcgaagatat gcctcgtatc ccagatgtga tgtacgtagt tgacccacat
    23161 aaagagcaaa tcgctgttaa agaagctaaa aaattgggaa tcccagttgt agcgatggtt
    23221 gacaccaata ctgatccaga tgatatcgat gtaatcatcc cagctaacga tgacgctatc
    23281 cgtgctgtta aattgatcac agctaaattg gctgacgcta ttatcgaagg acgtcaaggt
    23341 gaggatgcag tagcagttga agcagaattt gcagctttag aaactcaagc agattcaatt
    23401 gaagaaatcg ttgaagttgt agaaggcgac aacgcttaat ttatacaaat agtaattacc
    23461 taggagggcg gggcttagcc cggctctcct attttcaaaa aatataggag aattaaaatg
    23521 gcagaaatta cagctaaact tgtaaaagag ttgcgtgaaa aatctggtgc cggtgttatg
    23581 gacgctaaaa aagcgcttgt agaaacagac ggtgacatcg aaaaagcgat tgaattgctt
    23641 cgtgaaaaag gtatggctaa ggcagctaag aaagctgacc gtgttgctgc agaaggtttg
    23701 actggtgttt atgttaacgg taatgttgca gcagttattg aagtaaacgc tgaaactgac
    23761 ttcgttgcaa aaaacgctca attcgttgaa ttggtaaata ctacagctaa agtcattgct
    23821 gaaggaaaac ctgctaacaa cgaagaagct cttgctttga taatgccttc aggtgaaact
    23881 cttgaagctg catacgtatc tgcaacagca actatcggag agaaaatctc attccgtcgc
    23941 tttgcattga ttgaaaaaac agacgcacaa cactttggag cataccaaca taacggtgga
    24001 cgtatcggtg ttatttcagt tgttgaaggt ggagacgaag cacttgctaa acaattgtca
    24061 atgcacatcg cagcgatgaa accaacagtt ctttcttaca aagaattgga tgagcaattc
    24121 gttaaagatg agttggcaca attgaatcac gttatcgacc aagacaacga aagccgtgca
    24181 atggttaata aaccagctct tccacacttg aagtatggat caaaagctca attaactgat
    24241 gatgttattg ctcaagctga agctgacatc aaagctgaat tggctgcaga aggcaaacca
    24301 gaaaaaatct gggacaaaat tattccaggt aaaatggatc gcttcatgct tgataacact
    24361 aaagttgacc aagcttacac acttcttgca caagtttaca tcatggatga cagcaagaca
    24421 gttgaagcat accttgaatc agttaacgct tcggtagttg agtttgctcg ctttgaagtt
    24481 ggtgaaggta tcgagaaagc tgcaaacgac ttcgaagctg aagttgcagc tacaatggca
    24541 gcagccttga ataactaata atagaaagga agcaatttgc ttcctttttt gtatagggct
    24601 tagatttttc ctgaaattgt tttttcccca gtctcactcg attagaataa ttaaaacaaa
    24661 aagccctata ataagggcta agtgcattta ggagactata cttcaaattc atagtaagcg
    24721 cgtcataaca aggtatctat cctaattccc caacttccag tatactagat aaaaaataat
    24781 tatcggagga aaggtattgt actatgataa ttcaactaag tgatttaggt caagttcacc
    24841 ttgtttgtgg caagacagat atgaggcagg gaatagactc attagcctat gtagttaaaa
    24901 cccactttga attggattct ttctccggtc aagtctttct cttttgtggt ggacgtaaag
    24961 accgctttaa agccctttac tgggatggt
//
LOCUS       DAVDWM010000029        24940 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVDWM010000029 DAVDWM010000000
VERSION     DAVDWM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24940
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.029"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(112..561)
                     /locus_tag="VUW49_001364"
     CDS             complement(112..561)
                     /locus_tag="VUW49_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET7981272.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(563..1321)
                     /locus_tag="VUW49_001365"
     CDS             complement(563..1321)
                     /locus_tag="VUW49_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET7981273.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1436..3415)
                     /gene="pknB"
                     /locus_tag="VUW49_001366"
     CDS             complement(1436..3415)
                     /gene="pknB"
                     /locus_tag="VUW49_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET7981274.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKILPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3412..4152)
                     /locus_tag="VUW49_001367"
     CDS             complement(3412..4152)
                     /locus_tag="VUW49_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET7981275.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4167..5480)
                     /gene="rsmB"
                     /locus_tag="VUW49_001368"
     CDS             complement(4167..5480)
                     /gene="rsmB"
                     /locus_tag="VUW49_001368"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET7981276.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREDWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5473..6408)
                     /locus_tag="VUW49_001369"
     CDS             complement(5473..6408)
                     /locus_tag="VUW49_001369"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7981277.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6421..8817)
                     /locus_tag="VUW49_001370"
     CDS             complement(6421..8817)
                     /locus_tag="VUW49_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7981278.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFDSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQMVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8883..9197)
                     /locus_tag="VUW49_001371"
     CDS             complement(8883..9197)
                     /locus_tag="VUW49_001371"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7981279.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9222..9848)
                     /gene="gmk"
                     /locus_tag="VUW49_001372"
     CDS             complement(9222..9848)
                     /gene="gmk"
                     /locus_tag="VUW49_001372"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7981280.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9978..11591)
                     /locus_tag="VUW49_001373"
     CDS             complement(9978..11591)
                     /locus_tag="VUW49_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET7981281.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11782..12036)
                     /locus_tag="VUW49_001374"
     CDS             complement(11782..12036)
                     /locus_tag="VUW49_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HET7981282.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(12040..12294)
                     /locus_tag="VUW49_001375"
     CDS             complement(12040..12294)
                     /locus_tag="VUW49_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET7981283.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12532..13629)
                     /locus_tag="VUW49_001376"
     CDS             complement(12532..13629)
                     /locus_tag="VUW49_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET7981284.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13639..14385)
                     /locus_tag="VUW49_001377"
     CDS             complement(13639..14385)
                     /locus_tag="VUW49_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7981285.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFSMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14488..14841)
                     /gene="rsfS"
                     /locus_tag="VUW49_001378"
     CDS             complement(14488..14841)
                     /gene="rsfS"
                     /locus_tag="VUW49_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7981286.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIVANIREKVAQAGFKGSYVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(14854..15354)
                     /locus_tag="VUW49_001379"
     CDS             complement(14854..15354)
                     /locus_tag="VUW49_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET7981287.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15355..15948)
                     /gene="yqeK"
                     /locus_tag="VUW49_001380"
     CDS             complement(15355..15948)
                     /gene="yqeK"
                     /locus_tag="VUW49_001380"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET7981288.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVNVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(15948..16577)
                     /locus_tag="VUW49_001381"
     CDS             complement(15948..16577)
                     /locus_tag="VUW49_001381"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7981289.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16629..16940)
                     /gene="yhbY"
                     /locus_tag="VUW49_001382"
     CDS             complement(16629..16940)
                     /gene="yhbY"
                     /locus_tag="VUW49_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET7981290.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17154..18260)
                     /gene="yqeH"
                     /locus_tag="VUW49_001383"
     CDS             complement(17154..18260)
                     /gene="yqeH"
                     /locus_tag="VUW49_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET7981291.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(18263..18853)
                     /locus_tag="VUW49_001384"
     CDS             complement(18263..18853)
                     /locus_tag="VUW49_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET7981292.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(18805..19713)
                     /locus_tag="VUW49_001385"
     CDS             complement(18805..19713)
                     /locus_tag="VUW49_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET7981293.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19770..20330)
                     /locus_tag="VUW49_001386"
     CDS             complement(19770..20330)
                     /locus_tag="VUW49_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET7981294.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20469..21680
                     /gene="sstT"
                     /locus_tag="VUW49_001387"
                     /pseudo
     CDS             20469..21680
                     /gene="sstT"
                     /locus_tag="VUW49_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21748..22701
                     /locus_tag="VUW49_001388"
     CDS             21748..22701
                     /locus_tag="VUW49_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET7981295.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLNEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            22846..23226
                     /locus_tag="VUW49_001389"
     CDS             22846..23226
                     /locus_tag="VUW49_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7981296.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            23325..23546
                     /locus_tag="VUW49_001390"
     CDS             23325..23546
                     /locus_tag="VUW49_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET7981297.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLIKQDLSQYN"
     gene            23563..23877
                     /gene="trxA"
                     /locus_tag="VUW49_001391"
     CDS             23563..23877
                     /gene="trxA"
                     /locus_tag="VUW49_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7981298.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARSFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            24182..24715
                     /gene="queF"
                     /locus_tag="VUW49_001392"
     CDS             24182..24715
                     /gene="queF"
                     /locus_tag="VUW49_001392"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET7981299.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYSQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            24712..24801
                     /locus_tag="VUW49_001393"
                     /pseudo
     CDS             24712..24801
                     /locus_tag="VUW49_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT         6805 a         5815 c         4920 g         7400 t
ORIGIN      
        1 ataccaaaat cagccgcaat ttcttcataa gtgcggtatt ctcgcacata tagaaagcgt
       61 tgttaattta tttatctcat ttttcagaaa attcttttat ttctgtaaag tctacgatac
      121 tcgatgtgtt tttatataat gatacagtct gagaatcact gttccgctag ccattccaat
      181 agagattacc aaagccaaca tgacaaccaa ggtcgcactt gccagtgctt tattatagtc
      241 ccctgtcaca aaaaaggcag ttgttcggta ggagagataa cctggaacca gcggtgccaa
      301 aatggccaag ataaagacca cagcaggggt cttataaaga atacttaaaa tctggctgac
      361 acaagaacca ataatggctg caatgaaggt agctacaatg acattggtcg gttccttgag
      421 caagagatag attagccaga cagtcatgcc caaaatccct ccaggtaaga gcatagaccg
      481 ttgcacattg agtacgatta aaaaagtgat aatggcaaga aaacttgcta ctgcttgtaa
      541 taaaaaggtt gttagtgtca tattagttca tcaataccaa ggcgacagaa gttcctgccc
      601 ctaaagcgag ggtaatgagc agggattcaa acatcttact cataccagag tttatgtggt
      661 tggtcataat atcacggacc gcattggtca aggcaatacc tggtacaaac ggcatgaccg
      721 caccagctat aatcaaatct gccgttgaag gaaaacctgt gtagcgagcc caaaactggg
      781 caattatccc aaagacaaag gctccagcaa aggctgtcac aaagggaatt cggataaatt
      841 tttccacata gagggaaaag gcaaaaccaa ataaggtcgc cactcctgcc ccaagtgcgt
      901 cgtagatatt tccgctaaac ataactgaaa agaaaggagc actaaaggtc gcagccagag
      961 ttacctgcaa cttagtatag ggaaggggtt gagcttgcaa ggccgtcaat tgcttaaagg
     1021 ctgtttctaa gtcaatctgc cccccaacta gctgacgaga aatctggttc acatcgcaga
     1081 ctttttcgat gttataagaa gaggaggtca cgcgcttcat gcgcgaaata ttggtatttt
     1141 caatagagaa aaagatagcg gcaggcatgg caaggacatt gcaatccaca atcccctgcg
     1201 aatgcgcgat tcgaatcatg gtatcttcta cacgatggat ttctgagcca cttttaagga
     1261 gaatagtccc cgctagcata atcacatcaa tgacggcatt taattctttt gattcttcca
     1321 tgctttcctc cttttatcaa ctccctctat tctatcacaa atccggactc aaaaaaaatc
     1381 tttgccatga aatcatgaca aagattgatt actcattttg attatccatc tgcttttaag
     1441 gagtagctga agttgtttta ggtttgtaga ttgaaatctt gactctagtc ttattgaggt
     1501 ctaccttttc acctgctcta ggactttgtt caacaaccat gccttctgca ctacctgcag
     1561 gcgctgtcgt cacttctaca acttctatat tagcttcctt aatcccaaca atttgaatca
     1621 aattgttctt agtaaactcc aagctagaac caatgtaact cggcatggca acacttgtaa
     1681 cttttttagc tactgtcaag acaatttgag taggtttact cacatcataa gtcgttccgg
     1741 cacctggact ttgtttcata atcgttcctg gttcgctttc gctggactct tcttcctcta
     1801 tcttaatcaa attctcagga accttcttct gcttgagttc tgagattact tctgtagagt
     1861 tccgtccaat atagttccct aattgaatcg tcgtagcttt tttagctact gtcaaaacaa
     1921 tttgagttgc cttgctcaag tcataggtcg taccttctgg tagactttgc ttcaggaccg
     1981 ttccagcctc actctcattc gactcttctt cctcaatttt aatcaaatta tctggaactt
     2041 ttttctcttt taattccgca atgacatcag aggatttccg accgacataa ttactaattt
     2101 ggaaagattg cttgcctgat gagacaacca aattgatttt cgttccttct tttcgaccag
     2161 ttccagcgcc aggatctgta cggataatcc gcccttcttc caccttttca ctagcctctg
     2221 tcttctcctc accaatctca aaattggctt ttttgagcgt tgccttggcc tctgcaactg
     2281 tctgacctgc cacatctgga atggcaatgg ttgcaggagt tctggatagt atccaaataa
     2341 gagaagctgc caccaataca aggctggcca acaaaatcag gtaacgcatc ttaaatctat
     2401 gttttttcgg tgcttgtggt tggtaagttt cctctgtcac agcctggctt gggtttttga
     2461 ttgatttgtg ttctgtttgc gcttgaacct taggaataga tgtcaaggta ctctgagaaa
     2521 ccttcggcaa gatcttggta tctgccttgc tcgtttcatc aaagattaac ttactttcat
     2581 ttctacgatt gtaggacaag ctactagaca agtccacata catctctgaa accgagcggt
     2641 agcgattggt caacttttta gcagttgcct tgataataac attttctaaa gcctgaggta
     2701 cagatggatt ttctgcaata acggacggca ggggtttctg gaaatgctgg agggcaatgg
     2761 tcaccgcgct atccccgtca taagggatat ggcctgtcag catctcatag aaaataatcc
     2821 ccatggcata gatatcactc tgcacagtcg ccttcgaacc acgcgcctgc tctggtgaca
     2881 agtaatgaac tgagcccaac atcgagttag tctgggtcag acttgtctct gcaaaggcta
     2941 cagcaatccc aaagtctgtg accttggcag tcccatctgg tgtcaagagg atattttgag
     3001 gtttcaagtc cctgtgaaca attcctcgag tatgggccaa gcgcatagcc aagagaattt
     3061 gtcccatgat acggactgct tcttcattag aaagaggata atgttccttg atatagcgtt
     3121 tgaggtccag tccagccaca tactccatag ctaggtactg ttgaccgtct tcctcgccaa
     3181 tatctgttat ccgaacgata tgaggatggt ctagatctgc catagctctc gcttcacgct
     3241 gaaaacgagc tacagctatc gggtccgtct ggtagttggt cctcagaacc ttcactgcca
     3301 cttcttcccc atctaagatt aagtctttgg ctaggtagac atccgccata cctcctcgac
     3361 caatctgttt gacaatccga tagcgtccgg caaaaatctt gccgatttgg atcattctgc
     3421 atcctcctcg ttcatagaaa caagggcaac cgtaatgttg tctaaacctc ctgcattgtt
     3481 agcaaaacga acaagtgtct ccgttttatc tgctaaagga atatcactgg ttacaatatc
     3541 acgaatctca ctgcctgaaa tcatgttggt caagccgtca ctattgagca agagatagtc
     3601 acctgactca aggataactg tcccaaaatc aggctgaatt tcatcttttt gcccaataga
     3661 ctgggtgata atattttttt gcggatgagc ttctgcctct tctggtgtca attgaccagc
     3721 cttgagcaat tcattaacca aggaatgatc gctcgtcaac tgatggtatt cttctccacg
     3781 aatcaagccg atacgcgaat caccaatatg agcatagata gcctgattat caataatagc
     3841 aaggacttcc aaagtagttc ccatgcctct gtaagcttca tcctgaccaa gctggtgaat
     3901 cttttgattt tcaatttcta ggtaatgggc gaaccattca cgcacttcat tgactgtatc
     3961 gatctgggta tcaacccaag ctacacccag gtctgtgacc gccatttcac tagcgatatt
     4021 ccctgcgcga tgacctccca tcccatcagc taaaataatc atggtacgtc cagctctatt
     4081 gacatagtgg ttgacatagt cttggttatt tgttcgtttc tgaccaacat ctgttaataa
     4141 tgaaatttcc atgtgtcagt tccttcctaa tccgatatct tgcgaaattg actgatgaag
     4201 aatccatcac ttccatacaa ttcaggtgta atgaggatac agccgtcttt catgatatcc
     4261 ttacattcat gttctagttt tacctgctcg aactcgggat gactctctaa aaaggcctta
     4321 acgacttgaa aattctcctc tgagacgata gtgcaggtgc tataagttat tataccacct
     4381 ttgcctagta tttgacaaac actacctaat atttctaact gaatttcctg caaggacgcg
     4441 aaatctgccg tttctttatt gtatttgata tctggttttc ggcgcaaaag accgattcct
     4501 gaacaaggag catccaccaa aatcttatca aaggaatcct ggtcaaaaaa ctcatgcacc
     4561 tttctggcat ccaatttttg agtttgaacc cgatcttcaa ctcccagacg ttgggcattt
     4621 tcttgaatta aatccaactt gtggtcgtac aagtccagag cagtaacctg acctgtcgta
     4681 agataagagg ctatatgggc tgttttccca cctggagccg cacaggcatc aagcactcgc
     4741 tcatcacctt gtaaatcaag cgtcggagca accagctgac tggactcatc ttggatggta
     4801 atggctccat ccgcaaacaa attatgccct gcaaaatgcc cctgctcctt aaccagacca
     4861 gtggttgcta aaagggaatt attcgcctcc aacaaggctt ggatttcctc ttttcgactt
     4921 aggtctgtta cacgaatact ggctttgttt cgcactaaca ggctttcaaa gatggctttt
     4981 gctctctcct ctccgtattc ttccttgagt ttggcaacta gccaaactgg gagagaatag
     5041 gcaatggagt cacgcttgtt ttttcgcttg atgctagcaa tatctggcca gtcttcacgc
     5101 aagatacggc gaaggacagc gttgaccaat ttttcactgc cttttttacg gagtttggcc
     5161 aattccactg cttcattgac cacagcatga tctggaatct tgtccaaata gcggagttgg
     5221 taggcactca tgagaagaag gacatagagc cagctgtcta actggtctct gtcttcgata
     5281 aagtgggata ggtaccattc cagagtcagt ttacgggcta ccgttccata gactagctcg
     5341 gtcactaagc ccttgtctgc tgcagagagc tgactcccct tgaggtgttt atttaaggcg
     5401 atatttgaat atgcttggtt cacaaaaaca tcctctagca ctgctagagc taaacttcta
     5461 gccgtttcta ctttagtcac caaatcgttc tcctacagtc aatgtacgtc caactccgtt
     5521 gaggaaggaa gcaatgtcca tcttaggctt accagctggc tgcacttgtt tgagggatag
     5581 agccccttca gccgttgcga caatcaattc tttcttgccg atagagagaa tctcacctgg
     5641 atttccctga ccttctactg gtagggcttc ataaatctta aagcggtcgc ccttaaggaa
     5701 agtatgggca acaggccagg ggttcattcc acgaatttgg ttaaagagtt gacgattggt
     5761 tttgttccag tccagttttt cttcctctgg ctttatattt ggagagaagg taacctgact
     5821 cgtatcctgc ggttcaggtt tgatatcacc agcaatatag gcaggcagag tgtccaaaag
     5881 caaatcacga ccaactagcg ccaatttttc aaacaaggcg ccaacattgt cctcatctgt
     5941 gatcggaatg ctgcgacgag aaatcatatc tcctgcatcc atttccttaa ccatttccat
     6001 gatggtcaca ccagcttcct catccccttg aatcaaggca taatggatag gcgcaccacc
     6061 acggtgtcta ggaaggaggg aggcatgaac gttgacagca aagtccatgc tatcaaggag
     6121 tttgcttggg agaaactgcc caaaagcagc agtcacaatt ccatctgctc ctagcttcat
     6181 aagatcttcc atctctggac ttccagataa tttttcaggt tggtagatag atagtcctgc
     6241 ttccttggca gcctgcttga ctggggtttc ttggataact tttttacgac caacagcacg
     6301 gtctggctgg gtcacaacgg ctagaatttc gtaacggtca tctgtcaaaa gtccttttaa
     6361 gactgttgct gaaaagtcgg gggtccccat aaagattagt tttgtcatat cttctccttc
     6421 ttataaaaat tgctgcggct catggtcaat gctgagacgg agctcactat tttcccgttc
     6481 ttgagtcaag gccaggacct ggttgagggt cggccccagc tcatcttcta aacggtattt
     6541 aattaaaatc tggtaatgat agaggttgtg ggtacgggca atcggttttg gcgttggccc
     6601 cagaatggga ctggtctctg acaagcctga ccgcaaaatg ttcatgactt cataggcacg
     6661 tttgaaaacc tcttcttctt tcttgtgaga aagggtaata ccaatcgtga aatagtaagg
     6721 tggatagccg agttgtcgtc tgattcccat ttcataggca taaaagcctt cgtaatcttg
     6781 atccttggca aatcgaatag catagtgctg cggattgtag gactgtatca agacttgacc
     6841 tgctttttca gcccgacctg ctcgacctgc cacctgagtc aagagctgga aggttctctc
     6901 agaagaacgg aaatcaggca gattcaaggc cgtatccgca tttagaactc cgactagggt
     6961 aacattggga aaatccaaac cctttgcaat catctgagta ccaagtaaaa tatccgcttc
     7021 ccctcgccca aactggtcaa gcaaggcttg gtgactgcct ttctttcgag tcgtatccac
     7081 atccatcctc aaaatgcgag cttggggaaa gagttctgct agctcatcat aagccttctg
     7141 agttcccgtc ccatagtaac gaatactgtg gctcttacag ttaggacaga cctgaggaat
     7201 atccttcgag aaaccacaat aatggcagtt catagtcttg gtatccatat gcaaggtcag
     7261 agaaatatcg cagttgggac aagtatccac cgtcccacac tcccgacaca tgacaaagct
     7321 agaataacca cggcgattga gcatgagaac catctgctct tttttaacca gacggtcttg
     7381 gatagcctct agcaaaggag gcgtaaagtt tgacgtctca ttttgtccga tatagtctcg
     7441 aaagtcaatc acttgaacct cagggattgt agccaaagga ttggcacgtt gggttagacg
     7501 taagtgttga tagacgcctt tgccagcacg tgcccggctc tctaagctcg gcgttgcaga
     7561 tccaagtacc agagttgctt gattatactg agcccgtaaa atagctacct ctctggcatg
     7621 gtaacgggga ttgctgtcct gcttataagc cgcttcatgc tcttcatcaa taatcatgac
     7681 acccagattt ttcagaggag caaagatagc agatctggca ccaacaacaa cttgggcatc
     7741 gccacgctcc accttgcgcc attcatcata cttttcacca ttggataatc ctgagtgaag
     7801 aatggctacc ttgtccccaa aacgtgctat aaaacgctcg gtcatctgag gagtcaagga
     7861 aatctcaggt accagcaaaa tagctgtctt gcccttatcc agggcacctt ggataatctg
     7921 caagtaaacc tcggtcttcc cacttcctgt aatcccttga agtagaaagg gaggttgaga
     7981 actgccaata gaactcacaa ccgcatcacg cgcctgtctt tgttctggat ttaactccaa
     8041 aggtctactt gcttcaattc cttcaaaata agcagccgag cgtcgaactt ccttttggac
     8101 tatggtaaca gcaccttgat ccacaaagaa gttgacttgc tctcgcgagt aggactctaa
     8161 caagctagcc aaggaagcgc tctctggatg agacagcaga taatctctca gttccaactt
     8221 tttcttggca cgtgtagaaa tctcaacacc ttctaattga gcatggtcaa cctcatacca
     8281 agactgggtc ttgaccttct tttgatcgac tgcctgatat tccagaccaa gcaggccttt
     8341 tctagtcaaa cgcatcattt cagcttgctt acctaaatcc agagaagaaa aggctagcga
     8401 atcttctgaa tcaaacaggc gtactcgttc ttcctgactc aagccttcca gaggatagag
     8461 aatcttgtca tagctagaat tcagaaatcc cggaagcatg gccttgagga tagagatttt
     8521 gtaggagaag acagatttgc gtaactcctc agccagccag agttgttctg gcgtgagaac
     8581 aggagaaaaa tccagcacct ctgcaatatc ttttaaatct tgctccatct cttctccatc
     8641 tgattgggac tccaaaccaa gaacaatccc ttgaatcagg cgattaccct taccaaaagg
     8701 cacatgaacc cgcatcccaa cttccagcat tccctcaaat tcctccggaa tcctgtaact
     8761 atagggctgg tccgtctgca tcaagggcac atctacgata atcttagcta gggccatctt
     8821 ctcacctcct ccttgtcagt acattcttgc aatagaaaaa ataagattga gtccccccaa
     8881 ccttaaattt tttcaccatc ttctttttct ttagcaattt gctctttgat tttcttttct
     8941 tcttcttctt tgcggcgttt ttcttcttcg atacggcgac gcactgcttc acgttttcct
     9001 tctggatctg ggtgaattgt aacgtttcct gattcgattt cttctaaagc gcgaagagtt
     9061 gatttttcag acttgaaacc ttgagttgct ggggcacctg cttccaattc gtgggcacgt
     9121 tttgcttcca agattacgag tgaatatttt gaaggaacct tgtcgagcaa ggtatcaata
     9181 gagggtttta acatcatttg cttgtaccta ttttctaaat tttatcgggt agttggagat
     9241 tttggtaaca tctcctgata gtgaccaatg acacgatcca cacagaagtg ttctgcttca
     9301 atcacacatt tgacacgttc agcagctagg ggcacctgat cgttgacaat cgcataatca
     9361 tactcacgca tgagggcaat ttcttccttg gccttttcga ttcgttgggc aatcacttct
     9421 gcactatctg ttccacgacc taccaagcga tcttgcaatt catccaaatc tggtggtgtc
     9481 aggaagataa agacagcatc tggaaccttt ttcttgacct gaagagcacc ctgaacttca
     9541 atttcaagga aaacatcgat tcccttgtcc aaggtttcat tgacataggt cagaggagtt
     9601 ccatagtagt tgccgacata ttctgcgtat tccaacatct gtccttgacg aatcagctct
     9661 tcaaattctt cacgagtacg gaagaaatag tcaacaccgt ccacttctcc aggacgttgt
     9721 gcgcgtgtcg tcatcgatac agaatattga aattggtttt cagaactctc aaaaatctct
     9781 cttctaaccg ttccttttcc aacccctgaa ggaccagaaa aaacgattag taagcctcgg
     9841 tctgccattg tgtctccttt tagtcaatct gtgaaataac atttctctag aataatggca
     9901 aaaagccaga ttatccttta cagtctttct atctagtgta acaaaaaagc agtaattttt
     9961 caactgctct ttcttattta tttagcataa tctactgcac gaagctcgcg aatcacggtt
    10021 accttgatat ttcctggata atcgagattg ttttcaattt tcttacgaac tttgtgagcc
    10081 aagattgtga ctttgtcgtc cttgattttt cctggattga ccatgatacg aatttcacgt
    10141 cctgcttgaa gggcaaagct agtttgcact ccttcaaagc cgttagcaat ttcttccaaa
    10201 tcatggagac gcttgatgta gctttcaaga gactcactac gagcacctgg acgggctgcg
    10261 ctcaaggcat ctgctgcagc gacgataact gctatcacgc tctcagcttc aacatctccg
    10321 tggtgactag caatcgtatt caccacaact gggggttcct tgtacttacg ggccaattcc
    10381 ataccgattt caacgtggct accttcaacc tcatggtcaa tggctttccc gatatcgtga
    10441 aggaatccag cacgacgggc aagagccgca ttttcaccaa gttcgctcgc catgatacca
    10501 gccaacttag caacctcaat cgaatggcgc aaaacatttt gtccatatga agtacggaac
    10561 tgcaaacgtc ccataatctt catcaagtct ggatgaaggt ttggcgcacc aatttcatag
    10621 gcagcagcct caccgtattc acgaatctta ttgtcaatct cttgacggtt tttctcaacc
    10681 aactcttcga tacgagctgg atgtatacga ccatctttga gcaacatttc catagtcata
    10741 cgggcaatct cacgacgaat cggatcaaat cctgacaagg tcaccacttc tggtgtatcg
    10801 tcgataatca catcgacccc tgtcaaactt tcaaaggtac gaatgttacg accttcacga
    10861 ccaataatgc gtcccttcat agtatcgtct ggcagatgaa ccgttgagtt tgttgactcc
    10921 gctacatatt caccagcgat acgttgcata gcttgaacca agatgtcctt agccattttg
    10981 tcagaacgtt ccttgacctc ttgctcagct tcgcgaatgc gactggcaat ctccctggtc
    11041 aagttttcct ctgtctgagc caagataata tctcgtgctt ctgcctgaga cagagcacca
    11101 atacgctcta gttctgcttc tttttgtctt tcgacttcct ctaattgctc ttcacgcgca
    11161 tcaaggtttt tcgctctatc agaaatactt tgttcttttt gttcaagtgt ttgttcttta
    11221 ctcgtcaaat tgtcgtcctt acggtcaagg ctagtagctc tctctgtcaa acgactttcg
    11281 atttgtttga gttcttgacg ttctgatttg aattcagcgt ccacttcttc acggtatttt
    11341 ctggcttctt ctttggcctc caatagtgct tcttttttaa gagacttgct ttcacgtttg
    11401 gcttcattaa caagtaaatc cgcttcacgc tcagcttgtc cacgtaaatt agttgcttct
    11461 tgttcagcat ttaaaagcat caactctgca gcttcctgag atgatttcat cttagctgag
    11521 atgctgacat atccaatgac taaaccaatg atgacggcaa aaacagcaat cgcaagcgac
    11581 atgatttcca tgtttttacc tcattttatt gttattccga atgacataca ttcttttaca
    11641 ttctaccata aaaaagtgat tttcacaaac ctaaaataga atatgttttg gggaatttgg
    11701 aacacattta ccaaaataaa cttgttgttt agaaatagta gtttagtaga gacttgagaa
    11761 aaagcctacc tttcaataga cttagtaatg atctttaaag gacaagaaag ccacgctatc
    11821 tccatccatc atataaatca agcgattttc tgcatcaata cgccgtgacc aggctccttg
    11881 gtaatcatat ttgagtggtt ctggtttacc tattcctgta aagggatcac gttgaatatc
    11941 cttgattagt ttattgattc tttttaacgt tttcttatcc tgattttgcc agtagcaata
    12001 atctgcccag gcatcttctg taaacttgag cagcatttct tactcctcaa taacatggac
    12061 ctgagtactt ccagcacgaa cttgagccat tcctcgcaaa accttatcag aaagttcctt
    12121 attttgagca attctcaggg tttcttggat actatcccac tcactctttg aaaggactac
    12181 aatgtcctca tctggatttt tattgaccac cgtcaaaggc tcaaattcat catttacctt
    12241 cttcatgtag tcctttaaat gatttcggaa tgttgagtaa aggactgctt ccataaccat
    12301 acctcgtttt agctcttttc cactattata cacgaaaaga aagaaattgt caggaacttg
    12361 tacaagattt tcttttctat ctatttatac tcaatgaaaa tcaaagagca aactaggaaa
    12421 ctagccgcag gctgtacttg agtacggcaa ggcgacgttg acgcgatttg aatttgattt
    12481 tcgaagagta ttattcgtaa aaaatctcaa aaagcctacc tttcggtaga cttagtttgt
    12541 ttctattcta atcggcactc ttccaaaatt ttgctctgct atacttggct tccctagttg
    12601 gtaaatctgg tctgcttttt gggtcatagc atcccaaggt tctttgccaa ttcggctgac
    12661 tagactgacc tgacctttca aagacttgag atgttgccta cctttttcag taaatccaag
    12721 gacatgaatg gcttctggca agtcactttc tctagcctgc atcaaaatat aggtcaatag
    12781 gcgtctgaca cgcgccttgg tgtaacgttt ggtagtcact aactcgacca attcttccac
    12841 agactgggct gttttaatag cttctttaat gcgcacagcc atttcttgat tgacctgata
    12901 gatggtggtt aggtcgggat ttgacaagat ttgatagcgg agcaagggaa aatagtcttc
    12961 ccagatcacc ttactggcct gctcaaatag ggcaacagaa ggcataaaac gttctaagaa
    13021 atcttggtcc ttctgatgtt gacggagggc tgtcgccgag gcaaagtcca catctttatt
    13081 cacagaatgg taacctgccc cctgacgctg aatcggatgc agcttgatat tccgtcctgc
    13141 aaccgccttg gcataggcca gagcaagaac atgattgggg gtattacctg aaaaatcaag
    13201 accagcaaat tccttccaca tagcttgggt tttctgggga taggaaaggg aatcaggcag
    13261 attttccaca aatttctcca tctcagcacc tttctctgtg tataagtcag caattttctg
    13321 ataatctcga acttcctctg tcccaaaaac tagactatca atgcccaagc gatccaagat
    13381 atccatagct ccttgaccaa agaaatcagc tgcctgaaca ctgactaaaa agggcaattc
    13441 cactaccaag tctgcgccat tttccagcgc catctgggcc cttgtccact tatccacaat
    13501 agcaggctct ccacgctgca tgaaattccc tgacatggca acaattttca gtccctctgc
    13561 ctgatccagc aggtatttat ggccattatg aaaaggattg aactccgcga taatacctgt
    13621 tatggtcatg ctaatctcct acttctgcgc cacaaaaaac caacgggtgc tagtttctgt
    13681 tggctcctta tcctcaaagt ctgcatagag tttgaaggac ttgaatccag cctgttccag
    13741 caagatatca taggtcaaga tctcataggt cctctcctca tgtacttcat cgtggcgact
    13801 aaaggaaccg tcagcctcct tgataaagaa agtcagctca tgcacgatgg agtgaggagc
    13861 ttcgtcctca tatgtatccc aaagcatgga aaaatcttcc gcattttcat ggtaggaata
    13921 gccagggaat acttcatctg tctggtaggt cgagtgcacg tcaaagatga aaactccctc
    13981 ttcattcagc gcattgtaca cgtccttaaa gacgtcccct acttccacct catcctgcat
    14041 atagcagata gagtccgaat aacacgtgac aaaatcgtat ttccctgcct tggacaaatt
    14101 cagcatatta ccttctataa aagcaatctt ttgcttggct gaagttgctc tcttctccgc
    14161 aatcttcaac atatccgcac tcaagtcaag tccagtcaca tcaaaaccag cctgagagaa
    14221 gcgcactgat tgaattcctg tcccacaagc cagttccaag agtttctttc tctccttggt
    14281 cttaggcaaa tgacgcagag aaaaattcgt ccatttgtcg tataaactat cgtccatgac
    14341 cgcatcatag accgccgcaa aggtttcata agtcgccata atcatgatac taagagctcc
    14401 atggtaaaca ccactagccc tttacctttc tatcaatttt tctaaaataa gcaaagaaac
    14461 ccagttgact acaactgagt tcatcttcta agcaagagct tctgaaatat ctactgaatt
    14521 cgcctcatgc catagcttct ctagattata atgggcacgc atttcttctg aaaagatatg
    14581 cacgacaaca gcaccgaggt ccagtaagac ccagcctcca gctgcatcgc cttcgacata
    14641 gctaccttta aagcccgctt gagctacttt ttcacggata ttagctacga tagcgtccaa
    14701 ctgacggcta ttcattgagc tagtgatgac aaagtagtcc gtcacactag tcaaatcttg
    14761 tacatcaagt gcgaggatat cctccgcacg tttctcatca gccgctttca cgactagttc
    14821 tagtaattct ttttcgttca tttaatcctc tttttagaaa atatgtttgt aatctaggac
    14881 atctgcaaag cgctcctccc aaatgtcctc ataaaattca ttaatcgttt ctgctggaat
    14941 gtcatcccca tcaaaggttg tgacagcacc ttctggaaca ataagctgat agccatattc
    15001 aaaggcaacc ttgacagagg tatccacaca atattctgtc tgcataccac ataaaactaa
    15061 tttttcaatc ccctgcttat ccaagtattc ttttaagcca gtttctttga aaatactgtt
    15121 atatttcttc tgaaagacct tttcagcagg ttttcgattt aaaagcgcag ataactgcca
    15181 atcttctgat gtttgagctt cagagttctc aatatgttga acatagataa tttcaatatt
    15241 cttgcttcta gcctggtttt gtaaataaga aattttttcc aatagacttt ttgtctggaa
    15301 cccagtttct actaaaacat tctgaacatc aatgattata aaagccgtct tcatttagtt
    15361 ctctttcaaa tagtgcacaa aggcgttata ggtttcaagg gtttggggat agatggggaa
    15421 tccctgatga gctagatact ccacggtacg agctgtttcg taggccaccg ccttattgag
    15481 cgatagactt gcaatctcac gcgccacatc tactccagga aaggctcgat tgtgctcgat
    15541 atagtctgct acatagacaa ccttatctag gtctgtcatc tgaccagcac cgactgtatg
    15601 gacttcaatg gctcgcagga tttctgaatc atgcaaatcc aaatcttcct gaattttgta
    15661 aattccgacc ataccatgcc agacattatt accccagttt ttgaggtcag ggtctagctg
    15721 gtaacggtca atcaaagcta gaaattcctg atctgacaac tttttagcat agtcatgaag
    15781 aagacctgct agacttgctt tctcaacatt tacaccaaag cgctgagcta attccatagc
    15841 ggcacgctcc acacctagac aatgggttag acgtttctca ggtagaagct ctgccatttt
    15901 ttccaacaag gcctcacggg agcagttgat atagtcttga taggacatca gtagagcccc
    15961 tccttctcga tgtagtctag cactggctga ggtaggagaa agttgggttt ccgaccttgg
    16021 gcaatgaagt cccgcaccat gctggacgag atatccatga gcggtacgtc cacccagata
    16081 actggatagg aagtccctgc cttgtagcgt ggacgctgaa cccccacaaa ctgaaccatg
    16141 tcaaccagtt catcaattcg gtaccactta ggcagatagt caaccatgtc ggcaccgatg
    16201 ataaagtaat aatccgtatc tggattcttc tctgtcaaaa tcttcatggt gtcgtaggtg
    16261 taggaaatac ccttgcgctc caactcaatg gtttcaatga ctaggccgtc aattccctca
    16321 attgccaact caagcatctt gagacgatgg tgttcaggga tggtttcctt tttatcaacg
    16381 tgaggaggtt ggtattcagg catgagaaga acttgatcca gtcccaactg ttgccgtact
    16441 tgatccgcaa caatgagatg ggcattgtga acagggttaa aattcccccc taaaatccca
    16501 acttgtttgc gttttttctc cttgatttct ggctccaact ctaccttggt aaagggagtc
    16561 aatagttcga ttgtcatagg ctagtcccct ttatttctct ataaaaacag ttatttggag
    16621 tttcggtctt agatttcttt gactttctta gaaatcttgc gattttcttt cttgctagat
    16681 tgtttaaaca aaatcaagat gcgtcctatt ttttggactg tatccacacc gatttcttct
    16741 tccaaaattt cagctacttc gtggatgttt tcatctgtgt tttgtaagag agtaaccttg
    16801 attaattcac gcgcatcaag agcttgacgg acgctggttt tgatttggtc gttgagtcca
    16861 tttttcccga tttggatgat aggtttgagg gtgtgtgcct ggctgttgag gaaggcgcgt
    16921 tgttttgatg ttaatgacat aattttcttc cttgtttttt gatagttatt ttaggtggtg
    16981 agggacggtc ggaactaatt ttccaaagat ttcatctttg aaaaatggat ttcctgacct
    17041 cacaattgag ccttggtctc aattgtgccc cttgccaatc tacagattgg caatcacacc
    17101 acggcaataa ctatcgctat atgagctcac tttgttcgct cagcgatttt gttttaaata
    17161 attgcttttc gtgtgacgac ggcgacgccc tctggtgccc agacggcgac ttttgctatg
    17221 cctgttacac gaatccagcc taggcctgag atgactaggt ctgtcttatc tttaatggta
    17281 aagacatgct ggactagcct tgggaaatct tctttttcct tgctatttgg tggtgtcaga
    17341 agagttccca ggtgcttatc gtagaaagca ctagctcctt caagcttgct acgatggagt
    17401 ttgagttcat tatcaaagaa agcagtaaat ccttgctttt ctcctgctat aaagtcaaag
    17461 cgtcccaaac cacctaaaaa tagggtttgc tcaggattaa gctgataggt cttaggcttg
    17521 atttcctttt taggactgac atacttgagg tttttggccg tcaagtagtg agccatctgg
    17581 tggcggtgga taattcccgg cgtatcgtaa atataagatc cgtcgtcaag cggaatctct
    17641 attttgtcca aggttgtccc tgggaagcgt gaagtagtga tgacattctg atcacccgtg
    17701 atttcttgga taatagcatt gattagagtt gattttccaa cgttggtcac accgaccaca
    17761 tagacatcgc ggcccttacg gtagtgttca atcttgtcaa tgacttcctt aatggcatgt
    17821 ttattttgtg ctgaagttag gaccacatcg actggacgaa gaccttcttc atgggcacgt
    17881 ttcatgagcc actggctaat cttaccagac ttaactgact taggaaggat atctttttta
    17941 tttcctacca agaggacatc attgcccgag acgaaacgtg gtaaacctgg gatgacagat
    18001 ccattaaaat caaagatatc aatgacattg accactaaag catcactgtc tcccacctcg
    18061 tgcaagagct tgaggaaatc atcgttcgtc aactggacat ctgtgatttc attgtagtgg
    18121 cggagacgga aacagcgttg gcaatagact tcgccagtct ccaaaccttt ttcaagtgcc
    18181 gactgggggg taaaaccaag accagcttta tctgtcgtct gaatggttgc tccacaacca
    18241 atacagagaa tttcttccat agttaaattc cttttttata tgtaatcggt ccgtactttt
    18301 cagtgatttt tctcataaca cgacgctcac gagttcggtt aatctgcgtt ttgattgagt
    18361 catgttggac caagggtttg actaaaattg accgaatccc tgcacggtgg gctgctcgta
    18421 tatctgtcat gagctggtcg ccaaccatga ccacttcctt tttgtcatag tggaattcct
    18481 tcatagcacg gtcaatacca aatgtgaagg gcttcaaggc ccagtaaacg taatcaatcc
    18541 caaatttctc aactgctcgt tgaacgcgtt ttttggtgtt atttgacact acgataatgc
    18601 caatacccgc gtcccgaagg tcatgtagcc attgcttcat ctctggcgtt ccatcagggt
    18661 tgttccaagc aatgagggta ttatccaaat cgaccaaaac agccttgatt ccctgcgcct
    18721 gcaggcttgg gactgtcaga tcatagactg cttccacagc aaaatctggt atataatttt
    18781 caatcgccat tctggctcct tttcttaact ttttttcgca attttcctta gtaacaagga
    18841 taaaacaatc cacaaacctg cactagccaa actgatatag agccaagcat ggggctcatc
    18901 tgttaaaggt aagggaacat tcattccgaa aaagcctgtc acgactgcca aaacagctag
    18961 caagactgaa atgatagtca aggttgtcaa attgtcattc agattattgt ttagaatatt
    19021 gttgtaagag gctgaaagct gctgtaaaat ctgagagatt aggtctgtca tggataccag
    19081 ctgatgagcc tcaatcatgg catcatcaaa ctgttctctc tcaatctcat caaaactacg
    19141 atacaaggca tgaccttgaa tatgctctaa caaaatccga ttttgtttgg cagctgccgt
    19201 cagataaacc ataccagtct ccaaatcaga cagggcaaag aggtttttct tggtagttcg
    19261 ctggcgcaag aggtcattga cctcatccct actcttgtcc atctgctcaa tgacaggata
    19321 gtaggcattg ctgatgattt ccagactggc aaagagaaac ttataaatcg aaagcgtgtc
    19381 atggctctcc agataacgag tcatctgttc aatgacatag gcgttcttgg tattactaat
    19441 ggtaatcagg cgacgatgct cgacgataaa ggtcatggga aaagtctcat agtaggcctt
    19501 gtcctttttt acgtctagga cattatagat aaaggtaacc gtcccacttt cacggtggta
    19561 gtccatgtgg gcgcgctcgt ttctatccag tgcgtattca atggtttcct tgtccaaacc
    19621 gtaaatttca gaaaggtctt ctagtttatt caactttcct aggtctaggt ctatccaggt
    19681 acaaccattg cccaactgct tttctaaaac catcttttct ccttattaaa ctccctctat
    19741 tgtaccacaa attacttaaa ataacaggtc tagtctgtgc gtgagaaatt gctaacaact
    19801 gtgatatttc ggttagggac aaagtgaagg gcttgctcct cgctacgaag ctgtgtcgta
    19861 cgaattccca cactgacaac cttacccgat acagtaatcg gtccatttgt cagaacgacc
    19921 tcatctccca catccagttg acgttcaaag aggatgaaaa agccattgat gacatcagac
    19981 agaaagcctt gggctcccat accaatcgct accccagcaa taccagctcc agccagcaaa
    20041 ctagaaactg gcaaacctaa aatcgacaaa atgcagtaga gtaaaaagaa atatagcgta
    20101 taattaaaca cattttctag taaacgtgag atggtttttt ggcgtccgac atcatgacga
    20161 gacattttta gagaaggttt gacaattctc tgcaccatgg tatgaagcat ttttttagca
    20221 atataaaata caatcaaaag cagtaaaaga gaaatgacct tggttagaat attctcgata
    20281 atggttgtca catctagctt ttcaatataa gcctgaataa atttttgcat ataaaactcc
    20341 tttatttcat tataccatat tttcatcagt ctcattctcc ataaatattc aaaaaataga
    20401 cagaaaattc ttgattttgt ctatcattta tactataatc ataatcacta cacaaaaagg
    20461 agattattat gaaaagaatc attcatgctt ggaataaggc aagccttgtc aagcgtatct
    20521 tgattggtat gctttgtggg gaatcctagg actgaccctt cctaatctct cagggattgg
    20581 gctactcggg gatttattcg ttggaggtct aaaagctgtt gctcctatcc tagtctttgc
    20641 tctcgttgcc aatgcccttt cccaacatca aaagggacaa gatagcaata tgaaaactgt
    20701 tgttttcttg tatatcctat gatagggact ttcgccgctg ctcttgtagc tgtactagca
    20761 agtttcatcg tccctattga aattacccta aatagtgcca atactgaaat tgcaccacca
    20821 gatgggattg ggcaggtcct cagcaacctc ttgctcaaac tggttgacag cccagtcaac
    20881 gccctgctta ctgctaacta tattggaatc ttatcttggg cagtcatttt tggaatcgct
    20941 atgagagaag ccagtaaaaa tagtaaagaa ttgctaaaaa ctatcgctga cgtgacttct
    21001 aaaattgtcg aatggatcat caatctggct ccatttggaa tccttggtct tgtttttaaa
    21061 accatttctg acaagggagt cggaagcctt gccaactacg gtattttatt ggttctatta
    21121 gtaacgacta tgctttttgt tgcccttgtg gtcacccttt gattgccttc ttctttatga
    21181 gacgcaatcc ttaccctcta gtttggaact gcctccgtgt cagcggtgtg acagcctttt
    21241 tcactcgtag ttctgcggct aacattcctg tcaacatgaa actctgccat gaccttggac
    21301 tcaacccaga tacctattct gtttctatcc cactcggttc tactatcaat atggctggag
    21361 tagcgattac cattaacctt ttgacccttg ctgcagttaa cactcttgga attcctgttg
    21421 actttgccac agcctttgtc ctcagtgtgg tagcagctat ctcagcctgt ggtgcttcag
    21481 gtattgccgg aggttccctc cttcttatcc cagttgcttg tagccttttc ggtatttcta
    21541 acgatattgc catacaagtt gtttgggttg gttttgtgat tggtgtcatc caagactcat
    21601 gtgaaacagc ccttaactct tctacagaag atgtcctctt taccgccgtt gccgaatacg
    21661 cagcaacccg taaaaaataa ctcatcaagg caagcctgct tatgtcttgt cttttacgct
    21721 tttattctaa cttattagga aattcttatg tctattagcc aacgtacgac caagctcatc
    21781 ttagctacct gtcttgcctg cctgcttgct tattttctca atctttcatc agcagtttcg
    21841 gctggaatta tcgctctctt gagcctatct gatacgcgta gaagtacttt aaaactggct
    21901 cgcaatcgtc ttttttctat gcttctagct ctggctatcg gtgttctagc ttttcacttg
    21961 agcggatttc atatctggag tctcggcctc tatctggcct tctacgttcc tttagcctac
    22021 aagatgggct gggaaattgg catcacacca agcactgttt tggttagcca tctcttggtt
    22081 caagagtcaa cctctccaga ccttctagtc aatgaattcc ttctctttgc tattggtaca
    22141 ggatttgcct tgcttgttaa tctctatatg ccttcacgag aagaggaaat ccagcactac
    22201 cacacgctgg tggaagaaaa gttaaaagat atcctccagc gcttcaaata ctatttatcc
    22261 agaggagacg gacgcaaccg agcacagctg gtagcagaat tagacacgct tttgaaagaa
    22321 gccctcagac tggtctattt ggatcactct gaccacctct ttcaccagac cgactaccat
    22381 atccactact ttgagatgag acaacgacaa agtcgtatcc tgagaaacat ggcccaacag
    22441 attaacactt gtcaccttgc cgccagtgaa agcctgatct tagcgcaact cttttcaaaa
    22501 attgcaggtc aactgagcca gaccaatcct gcttctgatt tgctaaatga aattgaacgt
    22561 tatctggaag tcttccggaa ccgcagtctg cccaagacaa gagaagagtt tgaaacccgc
    22621 gccactcttc ttcaactcct acgtgaagcc aaaaccttca tccaagtaaa agttgatttt
    22681 taccaaaaat atagacagta aaaaataaaa ccactaaaaa acagagcttg ccagcattct
    22741 tgaaaagtag tataattatt gcatgcgcaa tcatcttatg acgcaaagac tgcccgttaa
    22801 tggactttct tctataatta tgactctaaa aagaaaggag acactatgac ctatttggaa
    22861 aaatggtttg acttcaatcg gcgtcagaaa gaaattgaaa gtctcttgga agagaccatt
    22921 gcccaacaga gcgagcaaag tctgaccttg aaagagttct acctgctcta ctatctggac
    22981 ttggctgaag aaaaatctct acgccagatt gacctgccag ataagcttca tctgagcccg
    23041 agcgcagttt ctaggatggt ggctcgcttg gaagctaaaa attgcggtct actcagtcgc
    23101 atgtgttgcc atcaagatag acgctctagt tttatctgcc tgacaaatga tgggcaaaag
    23161 acactggcct cgctacaaaa gactgtcgaa gaaagcttgg aagcaggttt ggatttcttg
    23221 atttaagatg attttaaaaa aagttgcgtg cgcaatcatt tttcttgaca ttctctttta
    23281 taaggagtaa aataaagtta gcattaaaca aaggagtttt aaatatgatt gaaattacct
    23341 atatagatgc cagcaagaac gaaagaactg tgactttcga gtcttatgaa gactttgaac
    23401 gttcgcaaca agcttgcctt atcggcgtcg cagactacta ccctgtccaa aaattaactt
    23461 acaagggtca taatttggac taccatggga cttatggaga tatcttcttc tatctcataa
    23521 aacaagattt aagccaatat aactaaaaaa aggagaaata caatggcaaa agcaattaca
    23581 gatgcaacat tcgaacaaga aacaaaagac ggtttggtct tagtagactt ctgggcaact
    23641 tggtgtggtc catgtcgtat gcaaggtcca atcttggaca aattgtctga agaactttca
    23701 gaagatgtct tgaaaatcgt taaaatggac gttgatgaaa atccaaacac agctcgttct
    23761 tttggaatca tgtctattcc aactcttctc ttcaaaaaag acggccaagt tgtcaaacaa
    23821 gttgcaggtg ttcacacagc agaacaaatc aaggccatca ttgctgaatt gagctaatca
    23881 aacgagagac caagtacttt ctttggtctc ttttttcttg ccctttgcca tttttcaaaa
    23941 aatatgctag actatagtag attgaaacta gaatagtaca cctctacttc taaaacattg
    24001 ttagaaatcg atttgactgt cctgatcgat ttgtcctgtt cttatttcat tttactatat
    24061 aagtagaata ttacgatgtt tgggatatgc catcgctaaa aaaactcgat ttggtatttt
    24121 ttagctccct aatgggagct ttttgcgtgc tctgaacatt tcccattttg gaaggagtac
    24181 tatgaaacat caatcagcct tggtcgtctt tagtggtggt caagattcaa caacctgcct
    24241 tttctgggcc atgcaacact atgaaacagt cgaagctgtc acctttgcct acagccaacg
    24301 tcatcacctc gaaattcaaa ttactagaga aatcgctaag gaacagggca ttcgtcacca
    24361 tatcctcgat atgtctctgc tgggacaaat cactgctcag ccagactttg cgacgattca
    24421 tatttcctac attcctgaca agctctgtgt cgagtcaaaa tccctcaaac tatatctatt
    24481 tagctaccga aaccacggag atttccacga aaactgtatc aacaccatcg ggaaagactt
    24541 ggtcaacttg ctagaccctc gctatttaga agtctgggga aaattcactc cgcgcggtgg
    24601 catttcaatc gacccctact acaactacgg taagcaagga actaagtatg agggcttggc
    24661 agaacaacgc ctcttccaac acgaccttta tccagagaaa attgacaacc gctaaactca
    24721 tactcaatga aaatcaaaga gcaaactagg aagttagccg caggttactc aaagcagtgt
    24781 tttgaggttg tagatgaaac tgacgaagtc agcttaaaac actgttttga ggttgtggat
    24841 agaactgacg aagtcagatc aaaacattgt tttgaggttg tggatagaac tgacgaagtc
    24901 agatcaaaac actgttttga ggttgtggat agaactgacg
//
LOCUS       DAVDWM010000030        24247 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVDWM010000030 DAVDWM010000000
VERSION     DAVDWM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.030"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..320
                     /locus_tag="VUW49_001394"
     CDS             <1..320
                     /locus_tag="VUW49_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981300.1"
                     /translation="GELIAPMTYEETMTSDFFEAWFQKFLLPTLTTPSVIIMDNARFH
                     RMGKLELLCEEFGYKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCS
                     CFN"
     gene            complement(527..2785)
                     /locus_tag="VUW49_001395"
     CDS             complement(527..2785)
                     /locus_tag="VUW49_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7981301.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGATREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(3014..3244)
                     /locus_tag="VUW49_001396"
     CDS             complement(3014..3244)
                     /locus_tag="VUW49_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET7981302.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3434..4117
                     /locus_tag="VUW49_001397"
     CDS             3434..4117
                     /locus_tag="VUW49_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7981303.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4117..4851
                     /locus_tag="VUW49_001398"
     CDS             4117..4851
                     /locus_tag="VUW49_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7981304.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            5005..5862
                     /locus_tag="VUW49_001399"
     CDS             5005..5862
                     /locus_tag="VUW49_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET7981305.1"
                     /translation="MTQIIDGKALAVKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            6004..6876
                     /locus_tag="VUW49_001400"
     CDS             6004..6876
                     /locus_tag="VUW49_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7981306.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALVAVKSGAGLVTVGTDRENIPALHSHLPEAMAFALQDKQLLKEQLEKAEVVLLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDTTASALTSFPKGTILVEKGPATRVWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            7158..7841
                     /gene="rpiA"
                     /locus_tag="VUW49_001401"
     CDS             7158..7841
                     /gene="rpiA"
                     /locus_tag="VUW49_001401"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET7981307.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7855..9066
                     /locus_tag="VUW49_001402"
     CDS             7855..9066
                     /locus_tag="VUW49_001402"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET7981308.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            9068..9610
                     /locus_tag="VUW49_001403"
     CDS             9068..9610
                     /locus_tag="VUW49_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET7981309.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9626..10435
                     /locus_tag="VUW49_001404"
     CDS             9626..10435
                     /locus_tag="VUW49_001404"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7981310.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10911..11891
                     /locus_tag="VUW49_001405"
     CDS             10911..11891
                     /locus_tag="VUW49_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET7981311.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEIAEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAEVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNTKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNDRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRFSSQPSAEPMENIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHT
                     NFQSKPWWQVDLAKEETIRQINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11916..12428
                     /locus_tag="VUW49_001406"
     CDS             11916..12428
                     /locus_tag="VUW49_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET7981312.1"
                     /translation="MSAQITINHKKARYVRIELKGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            13093..13803
                     /gene="deoD"
                     /locus_tag="VUW49_001407"
     CDS             13093..13803
                     /gene="deoD"
                     /locus_tag="VUW49_001407"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7981313.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13826..14350)
                     /locus_tag="VUW49_001408"
     CDS             complement(13826..14350)
                     /locus_tag="VUW49_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET7981314.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14403..14639)
                     /locus_tag="VUW49_001409"
     CDS             complement(14403..14639)
                     /locus_tag="VUW49_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7981315.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(14707..15627)
                     /locus_tag="VUW49_001410"
     CDS             complement(14707..15627)
                     /locus_tag="VUW49_001410"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET7981316.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15817..16220
                     /locus_tag="VUW49_001411"
                     /pseudo
     CDS             15817..16220
                     /locus_tag="VUW49_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16436..17017
                     /locus_tag="VUW49_001412"
     CDS             16436..17017
                     /locus_tag="VUW49_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7981317.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            17014..18291
                     /locus_tag="VUW49_001413"
     CDS             17014..18291
                     /locus_tag="VUW49_001413"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET7981318.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVHVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18309..18971
                     /gene="deoC"
                     /locus_tag="VUW49_001414"
     CDS             18309..18971
                     /gene="deoC"
                     /locus_tag="VUW49_001414"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET7981319.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18958..19347
                     /locus_tag="VUW49_001415"
     CDS             18958..19347
                     /locus_tag="VUW49_001415"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7981320.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19437..20489
                     /locus_tag="VUW49_001416"
     CDS             19437..20489
                     /locus_tag="VUW49_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET7981321.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20633..22168
                     /locus_tag="VUW49_001417"
     CDS             20633..22168
                     /locus_tag="VUW49_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981322.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            22161..23219
                     /locus_tag="VUW49_001418"
     CDS             22161..23219
                     /locus_tag="VUW49_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981323.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLTELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            23222..24178
                     /locus_tag="VUW49_001419"
     CDS             23222..24178
                     /locus_tag="VUW49_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981324.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         7057 a         4383 c         5504 g         7303 t
ORIGIN      
        1 atggtgagtt aatcgctcca atgacttacg aagagacgat gacgagcgac ttttttgaag
       61 cttggtttca gaagtttctc ttaccaacat taaccacacc atcggttatt attatggata
      121 atgcaagatt ccatagaatg ggtaagttag aactcttgtg tgaagagttt gggtataaac
      181 ttttacctct tcctccctac tcacctgagt acaatcctat tgagaaaaca tgggctcata
      241 tcaaaaagca cctcaaaaag gtattaccaa gttgcaatac cttttatgag gctcttttgt
      301 cttgttcttg tttcaattga ctatagcaga ttgaaataag atgagaacaa atcgattggg
      361 aaagtaaaat taatttctat aaatgtttta gcaattgttt cgtactattt cagattcagt
      421 ctactatata tcataatgga caaagggcaa gctgtcaaga aattacagtt attttagtaa
      481 aaaaagaagc agaaaaattt cattttctac tccttttcta taggatttat ttttcttttt
      541 cagaactttt aacttcagct tttttggcag atttaatctg aatgtttccc ttgctagtca
      601 taactgcttt gagatctttt tcgcttggat tttcaaggta gtagtcagtg attgtgtcct
      661 caatatagtc ttgaatagtc cgacgaagtg ggcgtgctcc catttttgga tcataaccta
      721 ggtcaaccaa cttttccttg accttgtcag ttacatccaa acgaatgttg ttgctagaga
      781 ggcgcttgtt aacatctgct agcatgagct cgacaatctg aaggaggtta tccttgctga
      841 gagccttaaa ttcgataatg ccatcaaaac ggttcataaa ctctgggcta aagaagttac
      901 cgagttcacc gaggacagaa ttggtacgtc cttctctagt agcaccaaat ccaacgctag
      961 cttcggtctt tcctgtacct gcatttgagg tcatgataat gatggcatcc ttgaagctaa
     1021 cggtgcgtcc ttgcccgtct gtcaaacgac catcgtccaa gacttgaaga aacatgtgca
     1081 taacatctgg gtgagctttt tccacttcat cgagaaggat gagagaatat ggattgtggc
     1141 gaactttttc agttaattga ccagcctcat catagccaac ataacctgga ggagcgccga
     1201 ccaacttagc tacactatgt ttttccatgt attcactcat atcaaagcga atcatactat
     1261 cagcagaacc aaaaagttcg atagccagtt gtttggaaag ttctgtctta ccgacaccag
     1321 ttggcccaac gaagaggaag cttccgattg ggcggttagg ggtaccaagt ccgacacgat
     1381 tacggcgaat agccttggca atcttatcga ctgcatcatc ttgaccaata acatgagact
     1441 tgagatcttc ggctagatgg atgagttgag attgttcttt ctctttcaaa tcaccaacag
     1501 ggatattggt tttctgctcg ataatgtgct caatagtttt ctcgctgatg ataggagtat
     1561 cctggtctgt gatctttttc ttttgcattt ccttatactt ggcaatctgg tcgcggaagt
     1621 aggccgcctt ctcaaaatct tcttctcgtg tagcttgaga cttgagattt tcagcctcaa
     1681 tcaagcgctg atcaattact ttaggatcca caaaattcaa ggtcaagttc atcttagaac
     1741 cagcttcatc taggaggtca atggccttgt caggcaagaa gcgatcttgg atgtagcgat
     1801 tggaaagagt tgcagctgct tcaatcgcag catcggtata ttgaacgtgg tggtaatctt
     1861 cgtatttctt ttgaatccct ttgagaatag tgattgtttc gtccaccgtt ggttcatcga
     1921 ctttaacagg ctgcatacga cgctcgaggg cagcatcctt ttcaatgata cggtattcat
     1981 tgagggtagt agcaccgact agttgcagtt ctccacgagc aagggctggc ttgaggatat
     2041 ttcctgcgtc catattacca tcactcgcag aaccagcacc aacaatttca tggatttcat
     2101 cgataaagag gatgatgtct tcacgtttgc gaatttcttc catgagtttt tgcatgcgtt
     2161 cttcaaattg tcctcgaatc cccgttcctt gaactaagct aaccacatcc agacggatga
     2221 cttgtttacc ttggagttta tgtggcacat cgccatcgac aattttctga gctagacctt
     2281 cgacaacggc cgtttttccg acaccaggtt caccgataag gacaggatta ttcttggttc
     2341 tacgattgag aatctcgatg acacggataa tctcatcgtc gcgcccaata acggggtcaa
     2401 tgtctccacg acgggcaatt tcagttacat taataccaaa ttcttccagc aggccttttt
     2461 cttggctagg tggcggagtt tgagcagatc cacgattttg ggaaccataa ccgccgtttc
     2521 caccgtaacc tccacctgat tgggttgggg gaataggagg agtattgcta gaaggtctga
     2581 aattgtttag atcattgaag aaatcaccaa agggatcgaa gtcacgattg ttcagatccg
     2641 tcataccttt gaagaggcta ttgttaggat ctgttttgat aatcttatag cagttttgac
     2701 agaggtcaat ttgtttttgt tttccattga gattggtgta aagatgaatt gttgagtcgt
     2761 taattttaca gttttgacaa agcatacatc tacctcattt ctttctttag cctgacttgt
     2821 ttaaaggtca aaaatagtca aattctatac tctcattata acaggattgg ggtgtaaagc
     2881 aagtaaaaag attgaagctt tgagaagttg ttacaatgaa aatttttgtg aatttggact
     2941 ataaaaaaat cctatttgtg agacaaaaag gattttactt agacatgcag ggacaatccc
     3001 agcttgtttt agattagtag agaagttcgt aaatttccat tgcgattaag tcaatgctat
     3061 caaacgtata cgtttcgcga tcttctacgt cacgaagggt aaagattggg ccgttttctt
     3121 cgtcgtggct gtacgcaact tctgcaacaa caactccttc gcgttcaaaa ttacgtttta
     3181 aatttccgcc atcgtttgcc attgcttcaa ggcggttgat gattctaacc aaatgtgatt
     3241 ccattttgat tctccttcat ttcttatcgt tactatttta ccataaagga ggccaacttg
     3301 actagaaaaa actaagattt ctcgctaatt ctgctagttt aaagattttt aaataaattg
     3361 gataaaaaat ttaaatttta attcaaaaca tgttataatc atacagtatt ctattttaga
     3421 aagcagtgtg actatgaatt tttctttttt acctaagtat ttaccttatt ttaactatgg
     3481 ggctgttgtg acgattctta tttctatctg tgttatcttt ttgggaacta ttttgggtgt
     3541 tgtcttggct tttgggcaac gttcaaagtt taaaccgctt gtttggttgg ccaacttgta
     3601 cgtttggatt ttccgtggga caccgatgat ggttcaaatt atgattgcct ttgctcttat
     3661 gcatatcaat gctccgacta ttcagattgg aattttaggt gttgattttt cgcgtctgat
     3721 tccagggatt ttgattatct ctatgaatag tggtgcttat gtttcggaga ttgttcgtgc
     3781 cggaatcaat gcggttccaa aaggtcagct agaggcggct tattcgctag ggattcgtcc
     3841 taaaaatgcg atgcgttatg tgattttgcc acaagcagtc aaaaatatct tgccagcatt
     3901 ggggaacgaa tttatcacca ttatcaagga cagctccctc ttatcagcta ttggggtcat
     3961 ggagttgtgg aatggggcta caacagtttc tacaacaacc tatctacctt taacaccact
     4021 tttatttgca gcattttact acttgattat gacctctatt ctgacagtag ccttgaaagc
     4081 ttttgaaaaa catatgggac aaggagataa gaaataatga cagaaacctt gataaaaatt
     4141 gaaaatttac ataaatcttt tggaaagaat gaagtattga agggcatcaa cctcgagatt
     4201 aaaagaggag aagttgtcgt tatcatcggt ccttcaggga gtggaaaatc taccttgctt
     4261 cgctctatga atttgttgga ggaagcaacc aaggggaagc ttatctttga gggagtcgat
     4321 attacggaca agaagaatga cctgtttgcc atgcgtgaga agatgggcat ggttttccaa
     4381 caatttaacc tctttcctaa tatgactgtg atggaaaata ttaccttgtc acctatcaag
     4441 actaaaggtg aaagcaggga agttgctgag aagagagccc aagagctttt ggaaaaagtt
     4501 ggtttgccag ataaggcaga cgcttatcca cagagtctgt cgggtgggca acaacaacgg
     4561 attgccatcg cgcgtgggtt ggctatggaa ccagatgttt tgctctttga cgaaccaact
     4621 tcagccttgg atcctgaaat ggtaggtgag gtattggctg ttatgcaaga ccttgccaag
     4681 tcagggatga ctatggttat cgtaacacat gagatgggat ttgcccgtga ggtggcagat
     4741 cgtgttatct ttatggcaga cggtgtggtt gttgaagatg ggacacctga gcagattttt
     4801 gaacaaaccc aagaacaacg gactaaagat ttcttgagta aggttttata atctatttta
     4861 aaatttcaag acaagattag tgaaaagttc aaccagagct ttttcttata gtttgaaact
     4921 ataggataac ctaggaaaga agtgttagag ggcactagca gtttttggta taatggaaga
     4981 tatttgaaag aaaagagaag tgatatgaca cagattattg atgggaaagc tttagcggtc
     5041 aaattgcagg ggcagttggc tgaaaagact gcaaaattaa aggaagaaac agctctagtg
     5101 cctggtttgg tagtgatttt ggttggggat aatccagcca gccaagtcta cgttcgcaac
     5161 aaggagaggt cagctcttgc ggctggttcc cgtagcgaag tagtgcgagt tccagagact
     5221 attactcaag aggaattgtt agacctgatt gctaaatata atcaggatcc agcttggcat
     5281 gggattttgg tccagttgcc attaccaaaa catattgatg aagaggcggt tttattagcc
     5341 attgacccag aaaaggatgt ggatggtttc catcccctaa acatgggacg tctttggtct
     5401 ggacatccag tcatgattcc ctcgacacct gcaggaatta tggaaatgtt tcatgaatat
     5461 gggattgact tggaaggtaa aaatgcggtc gtaatcggtc gttcaaatat cgttggaaaa
     5521 cctatggctc agcttctttt ggctaaaaat gcgacagtaa ccttggccca ctcacgaact
     5581 cataatcttg ccaaggtggc tgctaaagca gatattcttg tagtcgcaat cggccgcgcc
     5641 aagtttgtga ctgctgactt tgtcaaacca ggtgcggttg tcattgacgt tgggatgaat
     5701 cgagatgaaa atgggaagct ctgtggagat gttgattatg aggcagttgc accacttgct
     5761 agccatatca cgccagtccc tggaggtgtc ggtcctatga ccattaccat gctgatggag
     5821 caaacttatc aagcagcact tcggacattg gatagaaaat aagagaagca ttcgattaat
     5881 agagtgtatt ttctatagct atatctaaaa tggcagaaat gaatattaaa ttttagatat
     5941 aagattacaa aaggaggtct gcgtctcctt tttattgtat aataaaagtg agaggaaaaa
     6001 aggatgaaag tgattaatca aaccttatta gaaaaagtta ttattgaacg ttctcgttca
     6061 agtcataaag gagattatgg tcgtctgctg ctgcttggtg gtacttatcc ttatggaggt
     6121 gccatcatca tggctgcttt agtagctgta aaaagcggtg caggattggt gaccgttgga
     6181 acggacaggg aaaatatccc tgctctgcac agtcatttac ctgaggctat ggcctttgct
     6241 cttcaagaca agcaattgtt aaaagagcag ttggagaagg cagaagttgt tttgctggga
     6301 cctggtttag gagacaatgc ttttggagaa gatttagtta aacaggtctt tgctggctta
     6361 aaacagaatc agattttgat tgtagacgga ggtgctttga ctattcttgc taggacaagt
     6421 ctgtcatttc cttctagcca gcttatccta actccccacc aaaaggagtg ggaaaaactg
     6481 tctggaatta cgattgaaaa gcaaaaggaa gatacaacgg ctagtgccct gacttctttc
     6541 cctaaaggaa caattttggt ggagaaaggt ccggctactc gtgtttggca agctggccag
     6601 tctgattatt accagttaca ggttggcggt ccctatcagg caactggggg aatgggagat
     6661 actctggctg ggatgatcgc aggatttgta ggccaattcc gacaggccag tctctacgag
     6721 cgtgtagcag tagcgaccca tcttcattca gccatagccc aagaactatc tcaagaaaat
     6781 tatgtggtct taccgacgga aatcagcagg tatcttccca aaataatgaa aattatttgt
     6841 caacaagaga gggtaagcaa agataaactt gtttaaaata gacagataaa tctacttcct
     6901 tctcttaatt tatgattctt gaaatgcgcc tgctttaagg ctaaactaga cgtatcaaag
     6961 ttaagagttt gtttttgtaa ctggaaagag atgaacaaat caaatacata aaagataaac
     7021 tcatttttat tcgtttggta aaaataaaat gcatattttt aaagaaagat tgatgaaaaa
     7081 tgtagatttg atgtttttac ttgaataaaa agcggtttca tagtaaaata agaactgaga
     7141 atgatggagg taaatcggtg gaaaatctga agaaaatggc aggtatcaag gctgctgagt
     7201 tcgtgagcga tggaatggtc gttggacttg gaacaggctc gactgcctat tattttgtcg
     7261 aagaaatcgg tcgtcgtatc aaggaagaag gattacagat tacagctgta acaacttcta
     7321 gtgtgaccac taaacaggca gaaggcctta atatcccgct caagtctatt gaccaagtag
     7381 actttgtcga tgtgacggtt gatggcgcgg atgaagtgga tagtcagttt aatggaatca
     7441 aaggcggtgg tggtgccctt ctcatggaaa aggtggtcgc aacaccatca aaagaataca
     7501 tttgggtggt ggatgaaagc aagctggtcg aaaaactagg tgcttttaaa ttgccagtag
     7561 aagtggttca gtatggtgca gagcaggtct ttcgtcattt tgaacgagct ggctacaaac
     7621 caagtttccg tgaaaaagac ggccaacgtt ttgtgacgga tatgcagaat tttatcattg
     7681 acctcgcctt ggatgtcatt gaaaatccaa ttgcttttgg acaagaattg gaccatgtcg
     7741 ttggtgttgt ggagcatggt ttattcaacc aaatggtgga taaggtaatc gttgctggac
     7801 gagatggagt tcagatttca acttcaaaaa aaggaaaata gaagggggca taagatgtct
     7861 aaatttaatc gtattcattt ggtggtactg gattctgtag gaatcggtgc agcaccagat
     7921 gctaataact ttgtcaatgc aggggttcca gatggagctt ctgacacact gggacacatt
     7981 tcaaaaacag ttggtttgaa tgtcccaaac atggctaaaa taggtcttgg aaatattcct
     8041 cgtgaaactc ctcttaagac tgtagcagct gaaatcaatc caactggata tgcaacaaaa
     8101 ttagaggaag tatctcttgg taaggatact atgactggac actgggaaat catgggactc
     8161 aacattactg agcctttcga tactttctgg aacggattcc cagaagaaat cctgacaaaa
     8221 atcgaagaat tctcaggacg caaggttatt cgtgaagcca acaaacctta ttcaggaacg
     8281 gctgttatcg atgattttgg accacgtcag atggaaactg gagagttgat tatctatact
     8341 tcagctgacc ctgttttgca gattgctgcc cacgaagaca ttattccttt ggatgaattg
     8401 taccgtatct gtgaatacgc tcgttcgatt acccttgagc gtcctgccct tcttggtcgc
     8461 atcattgctc gcccttatgt aggtgaacca ggtaacttca ctcgtacggc aaaccgtcgt
     8521 gacttggctg tatctccatt ttccccaact gttttggata aattgaatga ggctggtatc
     8581 gatacttatg ctgtgggtaa aatcaacgat atctttaacg gcgctggtat caaccatgac
     8641 atgggtcaca acaagtcaaa tagtcatgga attgatacac tattgaagac tatgggactt
     8701 gctgagtttg aaaaaggatt ctcattcaca aacctagttg actttgatgc cctttacggc
     8761 catcgtcgta atgctcacgg ttaccgtgat tgcttgcatg agtttgatga acgcttacct
     8821 gaaattatcg cagctatgag agagaatgac cttctcttga ttactgcgga ccatggaaat
     8881 gacccaacgt atgcaggaac ggatcacact cgggaatata ttccattgtt ggcctatagt
     8941 cctgccttta aaggaaatgg tctcattcca gtaggacatt ttgcagatat ttcagcgact
     9001 gttgccgata actttggtgt ggaaactgct atgattgggg aaagtttctt agataaattg
     9061 gtataagatg acgcgctatg ctttgctggt gagaggtatc aatgttggtg gtaagaataa
     9121 ggtcgtcatg gcggagcttc gtcaagaatt gacaaacttg ggactggaaa aggttgagag
     9181 ctacatcaac agtggcaata ttttctttac ttcgatagat tccaaagccc aattggttga
     9241 aaagctagag actttctttg cagtccatta tccatttatt cagagctttt ctttactgag
     9301 tctagaggac tttgaggcgg aacttgaaaa tctaccagct tggtggagca gagacttggc
     9361 acgaaaagat tttctctttt acactgaggg tttggatgtg gaccaagtca tcgcgacagt
     9421 tgaaagttta gagctgaaag atgaagtgct ttattttgga aaacttggga ttttctgggg
     9481 gaaattttct gaagaatcct attctaagac tgcctatcag aagtacttgc tgaaggtgcc
     9541 tttctaccgc cacattacta ttcgtaatgc taaaaccttt gacaaaattg gtcaaatgct
     9601 aaaaaaataa taaaggagac acacaatgac atttttaaac aaaatccatg aaactgctac
     9661 tttcctgaaa gaaaagggaa ttgcagcccc tgagttcggt ctaatccttg ggtcaggact
     9721 tggagaattg gcagaagaaa tcgaaaatcc agttgtagta gactatgctg agattccaaa
     9781 ctggggccgt tcaacagtag tcggtcatgc tggtaaattg gtatatggtg aactggcagg
     9841 tcgcaaggtc ttggctcttc aaggtcgttt ccatttctat gaagggaatc ctctggaagt
     9901 ggtgactttc ccagttcgtg tgatgaaagt tcttggatgt gaaggtgtta ttgtaaccaa
     9961 tgcagctggc ggtatcggat ttggtcctgg taccttgatg gctatctcag accatatcaa
    10021 catgacgggg caaaatccat tgatgggtga aaacttggat gactttggcc cacgtttccc
    10081 agatatgtct agggcctaca caccagaata ccgtgccact gcccatgaag tggctaaaaa
    10141 acttaatatc aagcttgatg aaggtgtcta tatcggagtt actggtccga cttatgaaac
    10201 accagcagaa attcgttcct ataagacact gggagcagat gcagttggta tgtctacggt
    10261 tcctgaagtt atcgtggcag cccactctgg cttgaaagtt ctgggaattt catgtatcac
    10321 taactttgcg gctggtttcc aagaagaact caatcacgaa gaagttgtag aagtgactga
    10381 acgtgttaaa ggtgatttca aaggcttgct taaagcgatt cttgctgaat tgtaagaaaa
    10441 aagatttaaa agggggagtg cctctgtctt ttcaggattg actgcctatc cgtattaaag
    10501 aagaaacaga ggaatactat gagcttcttc ctgctcttat aactgaaaga agcggaagaa
    10561 taggtatgtc tgatctgata gccagcattg tgaaagacaa gattctagga tactagcatt
    10621 agcttcctag ccaagcagac tagtatgata aggagagatg agaatgaatt gactttctga
    10681 atttctcagt cttgtcatat atagcacaat gagatttcgc ttgagtctgc ttgtaaataa
    10741 acgaaaagaa agataagaaa taatgaaaat tggtcaacga attatgcgct ttggcataaa
    10801 aaattaagta tcggagttgt atctgttgta gtcggctttg atttctagct ccagctggaa
    10861 tttcagccaa tgaagtaaag caagatgtaa catctgaagt ggtaataggt gtgctagatt
    10921 ctaaggagga attgaaagag tcagaaaatg atgctccaaa actagaaact cctcttagag
    10981 aggagccaag actagctcct caaacgcttc cggaagcaag tgaagttctt gaaaacaaaa
    11041 gggaagagtc aaaagtagag atagcagagc cagctcaagc ggatgatatc cgcaaggttg
    11101 ttggggaatt agccaaggat ataagtatta ctaagttgta tatgacaggt cattctcttg
    11161 gatgctacct agctcaaatt gcagaggttg aagcttacca aaaatatcct gatttttata
    11221 accatgtatt gaggaaagtg acaactttca gtgctcctaa agtgattact tccagaactg
    11281 tttggaatac taagaatggt ttctgggatg ttggtttgga aagtcgtaaa ttagctgtta
    11341 gtggaaaaat taagcattat gtggttgata atgacaatgt tgtgactccc ttgattcata
    11401 atgatcgtga tattgttaca tttacaggta attcacgctt taaacaccgt tctcgtggct
    11461 attttgaaag tccaatgaat gatattccta actttaatat tggtaaacaa gctaccttgg
    11521 ataaacatgg ttatcgtgat ccgaaattgg ataaagtgcg attctttaag aaacaggctc
    11581 tgcctcgatt ttctagtcaa ccaagcgctg aaccaatgga aaatattgcc ttaggaaaac
    11641 aggttactca aagttcgaca gctttcggag gagatgctag aagagctgtg gatggcaaag
    11701 tcgatggtaa ctatggtcac aattctgtca ctcatacaaa cttccaatct aagccttggt
    11761 ggcaagtaga tttggctaaa gaagaaacca ttcgccaaat caatatttac aaccgaacag
    11821 acactgccca ggatagattg gcaaactttg atgtcattct tttagacagt tctggtaaag
    11881 aaattgagtg aaaacgtata acatctccta aagatgtgtc agcacaaatt acgattaacc
    11941 ataaaaaagc gcgctatgtt cggattgagc taaaaggcta taatgccctc agtcttgcag
    12001 aagttgaagt tttccgcttt atagctacga atggtgaaac ggcgatacaa gtttctaagt
    12061 cagttcaacc aatcagtcag actcctgtga aggataaaac attgacaatt caacacagtg
    12121 gagcttacat tgcccgctac tccataactt gggaagaagt tccagtagat aaagatggaa
    12181 accaagttgt tcgtagtcat tcttgggaag gaaacggtcg caaccagact gcaggttttg
    12241 tcctcaacct cccaatcaaa gaaaatatga gaaatctgcg agttaagatt gagaaaaaga
    12301 cgggcctact atggaataga tggcaaacaa tctatgaaaa cagaccaatt ttagctcaac
    12361 cccaccgtaa aattacccat tggggtacga cattgaattc caaggtgagt gacgatgatg
    12421 tcttgtaatc tgatggtaga atgacagtta gtttgtctag tttataagaa agtactacct
    12481 gagcttgaat agaactcagg tagctctctg tgaaagaaca aaattaatac tcaatgaaaa
    12541 tcaaagagca aactaggaaa ctagccgcag gttgctcaaa gcactgcttt gaggttgtag
    12601 ataagactga cgaagtcagt cacatatata atccaaggcg acgttgacgt gatttgaaga
    12661 gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa cacgagtaga
    12721 aaacttttct aaaaataaaa acgaaaggat gggtaaactg tattcgctga actgaatacg
    12781 ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc ctaaaagcag
    12841 tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc tattttccct
    12901 tcgctttttg atgggtttgg tatctttctc aatataaaat ataaaatata aaataaagaa
    12961 aggtagagcg tgtgttttga tttgaacacg agcggaaaac tcggaaaata gataatctga
    13021 ctgaaaaatc aggatttctc gtcagcttcc taattttcag tcgttttctt ctcgctcttt
    13081 gtatcataaa ttatgtctat ccatattgct gctcagcagg gtgaaattgc tgataaaatt
    13141 cttcttcctg gggatcctct tcgtgctaag tttattgcgg agaatttcct tggtgatgct
    13201 gtttgtttta acgaagtgcg taacatgttt ggttacactg gtacttacaa gggtcaccgt
    13261 gtatctgtca tgggaactgg gatgggaatg ccatctattt cgatttatgc gcgtgagtta
    13321 atcgtagact acggtgtgaa gaaattgatt cgtgtgggaa ctgcaggttc tttgaatgaa
    13381 gaggttcatg ttcgtgaatt agttttggcg caggcggctg caaccaactc aaacatcgtt
    13441 cgtaatgact ggccacagta cgattttcca caaattgcta gctttgattt gcttgataaa
    13501 gcctaccata tcgccaaaga acttggtatg actactcacg ttgggaacgt tttgtcatct
    13561 gatgtctttt actcaaatta ctttgaaaag aatatcgagc ttggtaaatg gggagtcaag
    13621 gctgtggaaa tggaagcagc agctctttac tatcttgctg cccaatacca tgttgatgcg
    13681 ctagctatca tgaccatctc tgatagcttg gtcaatccag acgaagacac aactgcagaa
    13741 gaacgtcaaa ataccttcac tgatatgatg aaggttggtt tggaaacctt gattgcagaa
    13801 taattatagc caaaaaggaa gccctttatg aattgtagga cttccttttc ttatccagaa
    13861 attgatctag ctctctctga tttcgaagga tagtgacttt atgtgaatat tcttggcaaa
    13921 gtttttggta attttctttt tgagttttgc tacgcccatc ccaaagaatc catctgataa
    13981 actcccactc aaagcgttca gggcaatctg ccgccatact ttctctgact tttccacggt
    14041 atttaagata acgcttaaag gctctaaaga gacaggtcaa tggcaaaaaa ttgagaaaga
    14101 tgatttggtc agcttcttgc attcgttctt ggtagtagca ccaagaataa ttaccatcga
    14161 tgacccaagc tttatgcttg gtgagaaagt tttttatctc ggttaacatc cattcgcagt
    14221 cactgtcttg ccaaccaggt tgaaattgga gtgtgtccat gtgcagtttt ggaatggagt
    14281 agtagttaga taacttttct gctagagttg acttaccaga accagaatat ccgataattg
    14341 cgattttcat tttctacctt ttcctatttg gagacaaaaa aacagcctct atggactgtt
    14401 tcttatttaa caagtttagc tgaaagacga gctttatcgc ggcttgcttt gtttttgtga
    14461 atcaaacctt tagtttctgc tttatcgata gctgagctag cagcacggaa aagttcttca
    14521 gatgggtttg cttcgaaagc ttttatagca gtacgcatag ctgatttttg agctgagttc
    14581 ttttcgtttt gtttaacgtt caattcagcg cgtttgatag ctgatttaat gtttgccaat
    14641 gttcttacct ccatatttac taactatacc attatatatg aaaacttacg ttttgacaag
    14701 ggaaaattac ttttttaagt aaatttcatc gatttcatgg ttcttgcttt tatgaagaat
    14761 cacttctgca cgatttctgg ttggttcaat ataattttgc agatttgtga gattgatact
    14821 gatccagacc tgatgggcaa aggactccac ttccccaatc ggcatctgag taaaacgata
    14881 atagtagcta tcagggtcgt tttgggctag gctcagcatt ttcaagaaac ggtccagata
    14941 ccaactttca atatcatcca ctccagcatc tacatagatg gaaaagtcaa agaagtcagt
    15001 gatatagaga cgatcgtttt gtggattttg aaagacatta attccctcaa caattacaaa
    15061 atcagcagct ttgacacttt gtttcttttc gggtacgatg tcgtaaactt catgagaata
    15121 gacaggaata tctacatctt gtccattttt gatgcggtcc aagaagttga gaagagcttc
    15181 catatcatag ctttcaggaa atcctttacg atttaaaatc ccctgctcaa tcaaggtttg
    15241 attgggatag agaaaaccat cagttgtaac caactcaacc gtagcatctg taaacgtacg
    15301 ggacagtagg atttgaagta ggcgactggt tgtggatttt ccaacggcaa cactcccaga
    15361 aaccccaata ataaaaggtt gagatttact ttcacgttgg aggaaaattc cttttgaaaa
    15421 ggctaaatct tccttagttc gcttgtaaat ctgaatcaga tgagccaagg ggagatagat
    15481 atctgtaacg tcttggagac tgatttggtc attaaaactc ttgatagatt ccaattcttc
    15541 ttctgtcaaa ggaggtgttg tctttcgatg taaagattgc caagtctggc ggctgatttt
    15601 ttcaaaatgt aaaaattcgt tggtcatttg ttttccccta gatattttgt ctatcatacc
    15661 ataaaaagct aaaaaaataa agcggtttct tgaatgtggt aagtcaatgg tatatagtat
    15721 aagtgatcaa tttattgatg aaataggaga tagattctac tgatttataa gcttataaag
    15781 ggggcttttt agttcacaac ttataggagg tgtactatga aaatcttaaa acgttacata
    15841 ttggaactct gttttatttt aagttttgct ttacctttta taaaaggaac caatgcagat
    15901 aatggtagat gctttgtgga aacctattac ggttttactt ttttgatgga acatgctatt
    15961 gtaacagctg tctttatctg ttcgttctta attgctttct tactaaaaaa cgatggacga
    16021 aatggattgc tgcgggtagt tattgctttt tagtcctacg gattgcgaca gagggttatt
    16081 tcttccacat gtcactagaa gatttgatac gactttggac aagtttggaa atcctgacac
    16141 aaacgtatca gttgggcttt tatctaaaca ttttgttagg aattttgttg ataataaagt
    16201 attttaaggt taagcaatag aaataataat ctgcctgatt atagatgata aactttttgt
    16261 ttttatctgg actgtgatat ttatagttct aaatggttta ttatttataa gtgttgaaag
    16321 agattgaaga aaatggcttc gtgacttgct ttcttcggtc tttttcgttt tattatcaat
    16381 ttgtaaacga tttacaattt gagtcaaatt atggtaaaat agtgtcatga gtaaaatgta
    16441 ctatgcagaa aatcctgacg ctgctcacga cattcatgag ttgagagtgg acttgttggg
    16501 agaaaaaatg acttttttga cagatgcggg tgtttttagc aagaaaatgg ttgactttgg
    16561 aagtcaactc ttgctcaagt gtctggatgt caaccaagga gagacagtcc tagatgtagg
    16621 ctgtggctat ggtccattag gtttgtcctt ggtcaaggtt tacggagttc aggcgaccat
    16681 ggttgatatt aacactcgtg ccctggattt agctcagaga aatgctgaaa aaaataatgc
    16741 aaaagcgacg atatttcaat ctaatattta tgcaagagtt caagggcgat ttgaccatgt
    16801 catttccaat cctcctatcc gtgcgggcaa gcaagtcgtt catgaaatca tcgagaaaag
    16861 taaagatttc ttggaaattg gtggagattt gaccatcgtc atccagaaaa aacaaggagc
    16921 tccaagtgcc aagtctaaga tggaagatgt gtttggcaat tgtgaaatcc ttaaaaaaga
    16981 caagggatac tatatcctta gaagtgtgaa agaatgagag cagttgattt aatccaaaaa
    17041 aaacgagacg gtcaagaact gacttcaagt gaaattgaat ggctgataga aggctatgta
    17101 tcaggaactg ttcctgatta tcagatgtct gcctttgcta tggctgttta ttttaaagga
    17161 atgacgactc gagaaatctc tgatttaacg atgaatatgg ttaagactgg gcaagagttt
    17221 gacctgtcag ccattgatgg tgtgaaagtt gacaagcatt ctactggtgg tgtcggcgat
    17281 aaggtaacct tgattttagc tcctcttgtt gctagcttcg gtgtgcctgt agcaaaaatg
    17341 agtggtcgtg gtcttggcca tacaggcgga acaattgata aattggagtc cattaagggc
    17401 tatcaagtag aacgtagtca agaggatttt attcgtcagg ttcaggatat tggagtatct
    17461 gtcattgggc aatcagacca gctggtcaaa gcagataagc ttctctatgc ccttcgtgat
    17521 gtgaccgcaa ctgtcgacac gattcctttg attgcgagtt cggtgatgag caagaaaatt
    17581 gcggcagggg cggatgctat tttgctagac gtgactgtcg gtgagggtgc cttcatgaag
    17641 acggttgatg aggcgcgtga gttggctcaa actatggtag atcttggtaa ggttgttgga
    17701 cggaagacag tagcagtgat tacggatatg agccagcctt tgggacgagc gattggaaat
    17761 cgtctggaaa tccttgaagc attggagatt ttgcaaggtc aaggacgtca ggatattacc
    17821 cactttattt gtgaattggc tcaaattatg cttggtttgg caaatgttaa caaaactgtt
    17881 gaagaagttc gccaacatct tgaaaatggt caagcactgg ctaagtttga ggaaatggtg
    17941 caagcccaag gtggagactt ggaagacctc tatcgtcctg tacatgtagc tcatgtggtg
    18001 gaaatccctg ctcaggaaac gggtgtcatt tcagctcttc cagctatgga ctttggtctt
    18061 tatgccatga ggctgggtgc tggtcgtgca gttaagtctg atgtcttgga ctatgaaaca
    18121 ggaattgttt ttgaaaagaa gattggagac tccgttcaaa agggtgaaat tgttgcaaaa
    18181 gtatacacaa atgaaaaaat tccacctcaa ctagttacag atttccaaaa atgtgttaaa
    18241 ataagtgatg aagtgaaaaa aatccgtgaa attgtagaaa tcatctccta aatggaggga
    18301 aaaccgaaat gaaattaaat aaatatatag atcatacgct tttaaaacaa gatgcaaaga
    18361 aaaaacaaat tgatagtttg ttgtctgagg ctagagagta tgactttgcc agtgtttgcg
    18421 ttaatccgac ctgggttgaa catgctaaaa aaggacttga aggcacagat gttaaggttt
    18481 gcacagtagt aggtttccct ttgggagcaa caacttcagc cgtgaaagca tttgagacaa
    18541 aagaagctat ccaaaatggt gcagatgaga ttgatatggt gatcaatgtt ggagctctca
    18601 aatcaggtaa tttagccttg gttgagtcag atattcgcgc agtagtggaa gcaagtggtg
    18661 ataagttagt gaaagtcatt attgaagctt gccttctgac agaccaagaa aaaattgttg
    18721 tttgccaatt ggcccaaaaa gctggggctg actttgttaa aacatctact ggcttttcaa
    18781 ctggtggtgc tacgatagca gatgttagat taatgcgtga aacagttgga tctgatatgg
    18841 gagtcaaggc tgctggtgga gctcgttctt atgcagatgc tcttgccttt gtcgaagcag
    18901 gtgcgacccg tatcggaacg tcagctgggg tagctatttt aaaaggagaa ttggcagatg
    18961 gcgactactg agttgattga actggcaatt gaaaccagca aacatgccta tgtcccctat
    19021 tctcactttc ctatcggagc agttttagta gccaaagacg gaagtgttta cacgggagtg
    19081 aatatcgaaa atgctagcta tcctttgacc aattgcggtg agagaacagc tgtttttaag
    19141 gctatatctg aaggccaaag agaattttca gaattgattg tctatggtca aactgaaaaa
    19201 ccgatctcgc catgtggtgc ttgtcgccaa gtaatggtcg aattttttga acaagattta
    19261 aaagtgacct tagtcgcaaa agataaatcg acggtcgaga tgacggtcgg ggaattactt
    19321 ccatactctt ttacagactt aaactagtct gagtcgctct ctgagtggca cggtccttgt
    19381 ggccaaccaa tccatacttg caacatcgtt gcacatctta tttaggaggt tcagtaatga
    19441 acaagaaaca atggctaggc cttggtctag ttgcagtggc agcagttgga cttgctgcat
    19501 gtggtaaccg ctcttctcgt aacgcagctt catcttctga tgtgaagaca aaagcagcaa
    19561 tcgtcactga tactggtggt gttgatgaca aatcattcaa ccaatcagct tgggaaggtt
    19621 tgcaggcttg gggtaaagaa cacaatcttt caaaagataa cggtttcact tacttccaat
    19681 caacaagtga agctgactac gctaacaact tgcaacaagc ggctggaagt tacaacctaa
    19741 tctttggtgt tggttttgcc cttcataatg cagttgaaga agcagctaaa gaacacactg
    19801 acttgaacta tgtcttgatt gatgatgtga ttaaagacca aaagaatgtt gcgagcgtaa
    19861 ctttcgctga taatgagtca ggttaccttg caggtgtggc tgcagcaaaa acaactaaga
    19921 caaaacaagt tggttttgta ggtggtatcg aatctgaagt tatctctcgt tttgaagcag
    19981 gattcaaggc tggtgttgcg tcagtagacc catctatcaa agtccaagtt gactacgctg
    20041 gttcatttgg tgatgcagct aaaggtaaaa caattgcagc cgcacaatac gcagccggtg
    20101 cagatattgt ttaccaagta gctggtggta caggtgcagg tgtctttgca gaggcaaaat
    20161 ctctcaacga aagccgtcct gaaaatgaaa aagtttgggt tatcggtgtt gatcgtgacc
    20221 aagaagcaga aggtaaatac acttctaaag atggcaaaga atcaaacttt gttcttgtat
    20281 ctactttgaa acaagttggt acaactgtaa aagatatttc taacaaggca gaaaaaggag
    20341 aattccctgg cggtcaagtg atcgtttact cattgaagga taaaggggtt gacttggcag
    20401 taacaaacct ttcagaagaa ggtaaaaaag ctgtcgaaga tgcaaaagct aaaatccttg
    20461 atggaagcgt aaaagttcct gaaaaataat ggatggaagt catttcttag gaagcggcca
    20521 agggccgctt ttttaagagt tgagacaaag tcattttgtc tcagtaaagc ttgctactag
    20581 acaaactagc aagttttatt gaaataaaat aagactctga aaggaagagc acatggcaca
    20641 cgaaaatgtc attgagatgc gtgatattac caaggtgttt ggtggatttg ttgccaacga
    20701 caaaatcaac ttgcacctac gaaaaggtga aattcatgca cttttaggag aaaatggggc
    20761 tggtaagtcc acgctaatga acatgttagc aggccttctt gaaccaacca gtggtgaaat
    20821 agcggtcaac ggtcaagttg tcaatctcga ctccccatct aaagcagcta gcttgggaat
    20881 cgggatggtt caccagcact ttatgttggt tgaagccttc acagtggctg aaaacatcat
    20941 tttaggtagt gaattgacta aaaatggtgt gctagatatc gctggagcta gcaaagaaat
    21001 caaggctctt tctgaacgtt atggcttagc tgttgaccct tctgccaagg tagcagatat
    21061 ctcagttgga gcccaacaac gtgtagaaat tttaaaaaca ctttatcggg gggctgatat
    21121 ccttatcttt gacgaaccaa cggctgtttt gactccatca gaaattgatg agttgatggc
    21181 tattatgaaa aatcttgtca aagaaggaaa atcaattatc ttgattaccc acaaattgga
    21241 tgaaattcga gcagtttctg accgtgttac agttatccgt cgtgggaaat caattgaaac
    21301 cgttgaaatt gcaggggcta ccaatgctga tttggcggaa atgatggtag ggcgttctgt
    21361 ttcctttaaa acagagaagc aagcctctaa accaaaagaa gtggttttgt ctatcaaaga
    21421 tttggtggtc aatgaaaacc gtggtgttcc agctgttaaa aatctgtcct tggatgttcg
    21481 tgctggagag attgttggta ttgcggggat tgatggaaat ggtcagtctg aactgattca
    21541 agccattaca ggtcttcgta aggttgaatc tggtagcatt gagctaaaag gagattcaat
    21601 tgtaggcttg cacccacgtc agattacaga actaagtgtt gggcacgttc cagaagaccg
    21661 tcaccgtgat ggcttgattt tggaaatgat gatatctgaa aatattgccc ttcaaaccta
    21721 ctataaagaa ccacatagta aaaatggaat tttgaactat tcaaatatta cttcttatgc
    21781 taaaaagctg atggaagagt ttgatgttcg cgctgccagt gaattagttc ctgcagctgc
    21841 actctcagga ggaaatcaac aaaaagcaat tattgctcgt gaaattgatc gagatcctga
    21901 tctccttatc gttagtcagc caactcgtgg gttggatgtc ggtgccattg agtatatcca
    21961 caaacgcttg attgaagagc gtgataatgg caaggctgtc cttgttgtca gctttgaatt
    22021 ggatgagatt ttaaacgtct cagaccgtat tgccgttatc cacgatggta agattcaagg
    22081 tattgtatca ccagaaacaa ccaataaaca agaacttggt gtcttgatgg ctggtggaaa
    22141 cttgggaaag gagaagagtg atgtctaaaa aattacaaca aatttcggtt cccttgattt
    22201 ctgtattcct aggaatttta ctcggagcca ttgtcatgtg gatcttcggt tatgatgcta
    22261 tttggggcta cgaagaattg ttctatacag cctttggcag tctgcgtggg attggagaaa
    22321 tcttccgtgc tatgggtcct ctggtcttga ttggtcttgg ttttgccgtt gccagtcgag
    22381 ctggtttctt taacgtcgga cttcctggtc aggctttggc aggttggatt ctcagtggtt
    22441 ggtttgccct gtcgcatcca gatatgcccc gtcccttgat gattctagca accatcgtga
    22501 ttgccttgat tgctggtggg attgtcggag cgattccagg tattcttagg gcctatctag
    22561 ggacgtcaga ggttattgta accattatga tgaactacat tgtcttgtat gtagggaatg
    22621 cctttatcca tgctttccct aaagacttca tgcaaagtac agattcgacc attcgtgttg
    22681 gggctaatgc aacctatcag acaccttggt tgactgagtt gactggtaac tcacggatga
    22741 atattggtat tttctttgcc atcattgccg ttgcagttat ttggttcatg ctcaagaaaa
    22801 caactcttgg ttttgaaatc cgtgcagttg gtcttaatcc acatgcttca gaatatgctg
    22861 gtatttctgc caagcggact attatcctat ctatgattat ttcaggtgcc ttggcaggtc
    22921 ttggtggagc tgttgaaggt ttgggaacct tccagaacgt ctatgttcaa ggttcgtcat
    22981 tagctatcgg atttaacgga atggcggtta gtttgcttgc ggccaactca ccaattggta
    23041 tactctttgc agccttccta tttggcgttc tccaagttgg ggctcctggt atgaatgcgg
    23101 cgcaggtacc atctgagctt gtcagcattg taacagcgtc tattatcttc tttgtcagtg
    23161 ttcattacct tatcgaacgc tttgtcaaac cgaaaaaaca agttaaagga ggtaagtaaa
    23221 gatgtctatt ataaccttgc tccccctctt ggtgtcttct atgctgattt actcagcacc
    23281 tctcatcttt acaagtatcg gtggtgtttt ctctgaacgt ggtggtgtgg taaacgttgg
    23341 ccttgaagga attatggtta tgggtgcctt ttctggagtt gtctttaacc ttgaatttgc
    23401 agaacaattt ggagcagcaa ctccatggct atccttgctt gtagcaggat tggttggtag
    23461 tgttttctct atcatccacg cagcagcgac ggttcatttc cgtgcagacc atgttgtcag
    23521 cggtacggta ttgaacttga tggcgcctgc cttggctgtt ttcttagtta aagttcttta
    23581 taacaaagga caaaccgaca acctaagtca aacttttgga cgctttgatt tcccagtctt
    23641 ggcaaatatc ccagtgattg gtgatatctt cttcaagtca actagtctac ttggttatct
    23701 ggcgattgcc ttctcattcc ttgcttggtt tattctcttt aagactcaat ttggtcttcg
    23761 tctccgctct gtcggtgaac atcctcaagc agcggatacc ttgggaatca acgtctacaa
    23821 gatgagatat ttaggggtta ttatttcagg ttttctaggt ggaattggcg gagcgattta
    23881 tgcgcaatca atctcagtta acttctcagt gacaactatt gttggacctg gatttatcgc
    23941 ccttgctgcg atgatctttg ggaaatggaa tccaatcggt gctatgcttt ctagtctctt
    24001 ctttggactt tcacaaagtt tggctgttat cggttctcaa ttgccgttcc tacaaggagt
    24061 gccagcggtt tatcttcaaa ttgcacctta tgttttgaca attcttgtct tagcagcctt
    24121 ctttggaaaa gcagtcgcac caaaagcaga tggtatcaac tacatcaaat caaaataagc
    24181 atacaaaaaa acgtcagtcc aatgactgaa ctacacccca aaagttagac agaaaaaatc
    24241 taacttt
//
LOCUS       DAVDWM010000031        24168 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVDWM010000031 DAVDWM010000000
VERSION     DAVDWM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24168
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.031"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VUW49_001420"
     CDS             complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="VUW49_001420"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET7981325.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKENHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1419..2195
                     /locus_tag="VUW49_001421"
     CDS             1419..2195
                     /locus_tag="VUW49_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HET7981326.1"
                     /translation="MNSKTPRFSILDFPEQLRSYIKGANLSDSSSHSSATVLYIDSGY
                     YLKIDQKGRLAQETKIASWFETEGLGTPVITYLSANKDFLLTKEAIGSNALAFLNQPE
                     RLCQTLAQALNKLHSLKPQSFPSENHLKRYKEKALKNYQKGTFYNKTLLPQFHIHSRE
                     EAYQLIQEKGYILKADAFIHGDACLPNFILKDASHFSCFIDLGLAGFSDRHIDLFWAI
                     WSLHYNLQDATYAELFLDYYGREYVNMDKLRLVAAFEAFR"
     gene            2205..2981
                     /gene="recX"
                     /locus_tag="VUW49_001422"
     CDS             2205..2981
                     /gene="recX"
                     /locus_tag="VUW49_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET7981327.1"
                     /translation="MKITKLEKKKRLYLMELDGQQTSYITEDTIVRFMLSRDKVISKE
                     ELTEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYSKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            3070..3603
                     /locus_tag="VUW49_001423"
     CDS             3070..3603
                     /locus_tag="VUW49_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET7981328.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(3961..4446)
                     /locus_tag="VUW49_001424"
                     /pseudo
     CDS             complement(3961..4446)
                     /locus_tag="VUW49_001424"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            complement(4484..4816)
                     /locus_tag="VUW49_001425"
     CDS             complement(4484..4816)
                     /locus_tag="VUW49_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981329.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADSISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEHYKRSERDDFLVNLSPNFYSLNIKGLEVIKNKQQKL
                     FEKSGKNS"
     gene            complement(4829..5602)
                     /locus_tag="VUW49_001426"
     CDS             complement(4829..5602)
                     /locus_tag="VUW49_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7981330.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            complement(6049..6804)
                     /locus_tag="VUW49_001427"
                     /pseudo
     CDS             complement(6049..6804)
                     /locus_tag="VUW49_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(6928..8550)
                     /gene="groL"
                     /locus_tag="VUW49_001428"
     CDS             complement(6928..8550)
                     /gene="groL"
                     /locus_tag="VUW49_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET7981331.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(8566..8850)
                     /gene="groES"
                     /locus_tag="VUW49_001429"
     CDS             complement(8566..8850)
                     /gene="groES"
                     /locus_tag="VUW49_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7981332.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(9120..9515)
                     /locus_tag="VUW49_001430"
     CDS             complement(9120..9515)
                     /locus_tag="VUW49_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7981333.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(9592..10353)
                     /locus_tag="VUW49_001431"
     CDS             complement(9592..10353)
                     /locus_tag="VUW49_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7981334.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(10386..11012)
                     /locus_tag="VUW49_001432"
     CDS             complement(10386..11012)
                     /locus_tag="VUW49_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET7981335.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVACLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(11028..11345)
                     /locus_tag="VUW49_001433"
     CDS             complement(11028..11345)
                     /locus_tag="VUW49_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7981336.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(11342..11641)
                     /locus_tag="VUW49_001434"
     CDS             complement(11342..11641)
                     /locus_tag="VUW49_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET7981337.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(11966..12157)
                     /locus_tag="VUW49_001435"
     CDS             complement(11966..12157)
                     /locus_tag="VUW49_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET7981338.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(12318..12992)
                     /locus_tag="VUW49_001436"
     CDS             complement(12318..12992)
                     /locus_tag="VUW49_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981339.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLAIIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(12998..13444)
                     /locus_tag="VUW49_001437"
     CDS             complement(12998..13444)
                     /locus_tag="VUW49_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981340.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELEAILPRYFSRVSKSTI
                     VNIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(13558..14061)
                     /locus_tag="VUW49_001438"
     CDS             complement(13558..14061)
                     /locus_tag="VUW49_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7981341.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(14058..14420)
                     /locus_tag="VUW49_001439"
     CDS             complement(14058..14420)
                     /locus_tag="VUW49_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET7981342.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(14486..16351)
                     /locus_tag="VUW49_001440"
                     /pseudo
     CDS             complement(14486..16351)
                     /locus_tag="VUW49_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(16383..16832)
                     /locus_tag="VUW49_001441"
     CDS             complement(16383..16832)
                     /locus_tag="VUW49_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET7981343.1"
                     /translation="MDKPMLVFKRFGHQIYLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(16956..17672)
                     /locus_tag="VUW49_001442"
     CDS             complement(16956..17672)
                     /locus_tag="VUW49_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7981344.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(18553..19968)
                     /gene="ply"
                     /locus_tag="VUW49_001443"
     CDS             complement(18553..19968)
                     /gene="ply"
                     /locus_tag="VUW49_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET7981345.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(19979..20311)
                     /locus_tag="VUW49_001444"
     CDS             complement(19979..20311)
                     /locus_tag="VUW49_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981346.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(20382..20990)
                     /locus_tag="VUW49_001445"
     CDS             complement(20382..20990)
                     /locus_tag="VUW49_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981347.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(21003..21461)
                     /locus_tag="VUW49_001446"
     CDS             complement(21003..21461)
                     /locus_tag="VUW49_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET7981348.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            21586..21985
                     /locus_tag="VUW49_001447"
                     /pseudo
     CDS             21586..21985
                     /locus_tag="VUW49_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22470..23303
                     /locus_tag="VUW49_001448"
     CDS             22470..23303
                     /locus_tag="VUW49_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET7981349.1"
                     /translation="MNPLKECYEKNGILIAAHRGTNGGNILQNTSKAYLNSIRHNADM
                     FEVDVIRSKDGEFFAFHDGQEPLVFGEKIDIRTLSSEEIQRKECFNSLNEPILQKIEK
                     ISDVLKNFEGQSLINIDRSWFYWDTFLDKLKIYPSLNQVLLKSPVKKELLEMLRDSGL
                     NVMYMPIIKTIEELDFVLSFEGINTVAVELVFQDLENPIISKKIIDDLHEKGLLIWVN
                     ALTLSDSIILSAKIDDDTAIAHDGESWGKLVSIGFDIIQTDWPLLLYQYLVEKNKKIK
                     K"
     gene            23330..>24168
                     /locus_tag="VUW49_001449"
     CDS             23330..>24168
                     /locus_tag="VUW49_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7981350.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNDLKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQA"
BASE COUNT         7497 a         5024 c         4182 g         7465 t
ORIGIN      
        1 ccttttttaa acttttttta ttaattttac aacagcttca gttcgagctg tatgtgggaa
       61 catatcgacc gactggatat aatgaagatc atagacttct actaagcgta ccaaatcacg
      121 agccaaggtc gaaacattac aagaaatata aaccattttt tctggtacat aagtaagaat
      181 agtatctaat aacttatcat ccagacctgt acgtggtggg tcaacaatca aagcatctgc
      241 tcggtagcct tccttgtacc aacgaggaat aatctcttct gccgttccag cttcataatg
      301 agtattgtca aatcccattc ttttagcatt tcgcttggca tcttcaatag cttctggaat
      361 aatatccata cctctgagtg tttttacttt ctttgcaaag gcaaatccaa tcgttccaac
      421 tccacaataa gcgtcaatca aatggttttc tttatcaaca tccagcgctt ttactgcttc
      481 gctatagagg acttctgttt gctcaggatt tagttgataa aaagctcgag gggatagtga
      541 aaattcataa ttgagtacac cttcttgaat actctcttgc ccccagataa tctctgtctt
      601 ttcaccatat atctcactgg ttttagctgt atttgtatta acagctactg tcacaacttc
      661 tgggaaatct ttaaccaact cttttaccaa ttgagttaaa ttaagctggc ggtttgtaac
      721 aataataatc tgaacctgtc cggtctttct cgcgcgtcgg accataatag tacggacacc
      781 tagaactttt ctctcatccg tgattggaat ctggtgataa gtaagtaatt ctgctaagcg
      841 attagcaatc acttgggttt ccttatcttg taccaggcag tctttcaact ctactaaata
      901 gtgagagttt tgtgcatata agcccgcctt gacctgattt ttaaattttc gagtctgaaa
      961 ttgtaactta gctctgtaat attttggttc ctgcattcca atagttggac gaatttcata
     1021 attttcatat cctgcaggag caaatttttt cagcgcttga tgaagtaagt ccgtcttgaa
     1081 ctccagctgc ttatcataat gcaggtgcat gatttggcag cctccgcatt cattataaat
     1141 agtacaagat ggcacaattc gaaatttaga cttcttgttg accttcagta attttgcttc
     1201 aacaaagttg cgtctaatag aagtaatctg acaatagata tcttcgcctt tgagagctcc
     1261 tggtacaaag actaatgttt tttggtaaaa gccgattccc tcaccgttaa ttcccatgcg
     1321 cttgattttt aatggtattt tttgtttcac tttcagattc atatgtctat tataccatgg
     1381 agtttctaag atatttaaga atatgttaca ataaaagcat gaattcaaaa acacctcgct
     1441 ttagtatact agatttccct gaacaattac gaagctatat caagggagct aatctctctg
     1501 atagttcttc tcattcaagt gcaactgtcc tatatataga ttcaggttac tatttaaaaa
     1561 tagatcaaaa aggcagatta gctcaagaaa ctaagattgc aagttggttc gaaactgaag
     1621 gattgggaac tccagtgatt acctatttat cagcaaataa ggacttcctt ctgactaaag
     1681 aagccattgg cagcaacgct ctcgcttttt taaatcaacc cgaaagactc tgtcaaaccc
     1741 tggctcaagc attaaacaaa cttcacagtc ttaaaccaca atcttttccg tcggaaaatc
     1801 atctgaagcg ttataaagaa aaagccttaa aaaactatca gaaggggact ttttataaca
     1861 agactcttct gcctcaattt cacattcaca gtcgtgaaga agcttaccaa ctgatacaag
     1921 agaaaggtta cattctcaaa gctgatgctt ttattcatgg agatgcctgc ctgccaaatt
     1981 tcatcttgaa ggatgcctct catttttctt gctttattga cttgggattg gctggtttta
     2041 gtgaccgaca tatcgacctc ttttgggcaa tttggtccct tcattacaac ctacaagatg
     2101 ctacgtatgc tgaattattt ctcgactatt acggtagaga atatgtaaat atggataaac
     2161 tacgacttgt tgcagctttc gaagcattta gatgaaagga aattatgaaa atcacaaaac
     2221 ttgaaaagaa aaaacgtctc tacttgatgg aactagatgg acagcaaacc tcttatatca
     2281 cggaagatac cattgtccgt tttatgctgt ctcgagataa ggtcattagt aaagaggaac
     2341 tgaccgagat tcaggatttt gcccagtttt cttatggtaa gaatctcgct ctctaccact
     2401 tatcctttaa agcacgcact gaaaaagaag tcagagaata tctgaaaaaa tacgatattg
     2461 ataaaaacat cgtttctcaa gttattgcta atcttaaaga agataagtgg attaatgatg
     2521 gtcagtacgc ttatgctatc atcaatacca atcaactttc aggagacaag ggtccttatg
     2581 tgctgactca gaaactagct caaaaaggga tttcaaaatc tactatagaa gagaacttga
     2641 aagaatttga tttttctgaa gttgctcaac gtgtagctaa taaactattg aaaaaatatg
     2701 agggaaaact tccatctcgt gccctgcaag ataagattat ccagaacttg actaacaagg
     2761 gcttctctta ttctgatgct aaaattgcct ttgacgactt agatagccaa gttgaccaag
     2821 aaacgactca agaactcatc ttcaaggaac ttgataaaca atatagtaag tatgctcgaa
     2881 agtatgaagg atacgaactg aagcagcgtt taactcaagt tttagcacga aagggctacg
     2941 atttttcgga tatagcaagc gctcttagag aatatcttta atatttccaa gtgaaattca
     3001 cagattttag gtaattttat ggtacaatag taaatgataa acttataaat tgtagaaagt
     3061 tggttagtta tgaagcttcc aaaagaaggc gactttatta caattcaaag ttataagcat
     3121 gatgggagtc tccaccgaac ttggcgggac accatggtac taaaaacaac agaaaacgcc
     3181 attattggtg tcaacgatca tacacttgtt accgaaagtg atggtcgtcg ttgggtcact
     3241 cgagaaccgg ctattgttta ctttcacaag aaatattggt ttaatatcat tgccatgatt
     3301 cgtgataatg gaacttctta ctattgcaat atggctagcc cctactatct ggatgaagaa
     3361 gcactgaagt atattgatta cgatttggat gttaaaattt ttacagatgg ggaaaaacgt
     3421 ctcttggacg ttgaagagta cgagcgtcac aaacgcaaaa tgaattattc tgatgacttg
     3481 gactatattt taaaagaaca tgtcaaaatt cttgttgatt ggattaacaa tggacgtggt
     3541 cctttctcag aagcctatgt aaacatttgg tacaagcgct atgtagaact aaagaatcgg
     3601 taaagttgtc aaaggtcagg aacaaaatcc tgaccttgtt tttttggtag tataaaataa
     3661 attgtgtaaa cacaaaaagg aataaatcca ttatagcaga gttacaacac attactagaa
     3721 agagatttat gcagaattcg taaaatatat aaaacaaata gatatactat tattttttaa
     3781 tgtctttatt cctagtaagt ttacaacaaa cttatatcgt ttaatacaaa aaatacgaaa
     3841 atgattagta taaaaaatac aggtcaactt cgtatcaaaa acattttttt gtatttccac
     3901 atagcatcaa tcatttagta caatatttta aaaacaatat cttaacagag aaaatgatat
     3961 ttaatctatc agctcattta atcgtttttg aaaatataaa gcaatacttt tagattcaat
     4021 aaactcaaaa tattttatga cattgtccat ttgtttaatt ccagattctt tagattctgt
     4081 caggtatatt ttatatcctt ctacaaattt gaatgtagat ttttcaaaaa cttttgtgtc
     4141 atcttttaat aatttttgta atttcatttc aaagtagtct gcatatatta gatgtgaatt
     4201 ttcaaggcaa cagaaccaaa aatttaaaca agctgacaca actaaatttc tattcttgcc
     4261 gattttttcg taataatgtg tacgctctag tatttctttt ccgaccatgt aaatataatc
     4321 tatttcaaaa agatgatata aattaccaat cagcgttatc tcataggact cccatcgttc
     4381 cttctggaat aaatagtcat atactaaatt tagatcatct ctactaacta catgattaca
     4441 atctagctga tttcataatc cctgaaccat aattgtattt attttatgag tttttcccac
     4501 tcttctcaaa aagtttttgt tgcttatttt ttattacctc caatccttta atattcaaac
     4561 tataaaaatt tggagataaa ttaaccaaaa aatcatcacg ctccgatctt ttataatgtt
     4621 ctaagtgatt acaaaaattc tctatcgaaa cattgatatt tgataataat tcaaaaaatg
     4681 ttgaaagaga aatttcattt tctccccttt caaatctaga aagttgagat tttgatatag
     4741 aatcagctgc agcatactct aaagaatatc cgtttaattt tctaaaatat cgaaaagctt
     4801 ctccgtaatt tttcacactt tcattccctt aactttcttt tcgagcgata aacataccat
     4861 attctttagg aatgatcaac ataccattat taaactcact ttccaatctt ttacgtatta
     4921 tttcctcttc tagtgaacct atttctgaaa ttcctttaaa cgattggatg tatttaacta
     4981 ggtcatctat actagttacc tgtaactcat catcatagag taatgtatcg acagagttaa
     5041 aatacctact caggtacctt ttaccatttt gtaaagtaaa cgttctattt tctaaatctt
     5101 gattaacctc atctttaaat aaacttgcca aataatttac aactccattt tcaccaaatg
     5161 tagcacagta aaaaattcct ccggttttta agaccctatt cacctcagag agagctttag
     5221 gaatatcatt cacatgatgt agaagcatat tagcaataac aatatcaaaa gtttcattct
     5281 caaaagaaat cttttgaata tccattatct cataattgac attgtttcta tttccaatca
     5341 ctgacttcgt tgattttacc atatctttag aaaaatctgt aacaatcaac tgcttcattt
     5401 tatctataga atcactatta cttttccaca attctcctgt accacatccc aattctagaa
     5461 cctttacttc atcagtgatt tggtagttag aaaatatcca attattaaat cccaatttat
     5521 tttttgaata cttcttatgc aattctactc gaatatcaag attatcatga cttttatatt
     5581 gttcaattac tttatcaatc attttaaatg acctccactc tataatttgg cattatatca
     5641 caagaataaa gatagtgtta aataagttgt ataaatgcaa aaaaggaata ttccgttata
     5701 gcagagttgt aacacatttc tataaagaaa tttattccta cgactcagtt tggcatgctt
     5761 aacttcctaa ctcaaaaaca aagcatcgat taatttatcc gttcttcttt tgaaacagat
     5821 ttgaactgat aattgctcgt tttaatttaa gaagctcttt tgagcaaaac ttcatttttt
     5881 taagagtcca acaatttctg tctatcgctt ttctactagc aaaaaaacct accttgaggc
     5941 aagttttcat ttatatatct aatttcgtaa tagtatagta aaatgaaata aggacagtca
     6001 aatcgatttc taacaatgtt ttagaagtaa aggtgtacta ttctagtttc aatctactat
     6061 aaatttgata tgagaatagt ctttctcaac atccttaccc aacaagaatg ctgtagcgtc
     6121 cttcttaact tgaaccaggt caatattctg ggcttgggct gcatagacgc agccacaata
     6181 acattgacga tagatatcat actcctcaca catctctact gaacgtttgt agccttgatt
     6241 tttcttgaaa tcgttgggaa gatagtgggt cgtgtaaatt ttttgcacat cgattccgat
     6301 gctgttgatg gtttgagaat tcttatgagg actgatggtc aaggctgaac caaagtagtc
     6361 aaagcccaag tccatagcca cttgcgctgt tttatccagt cggtagtcaa aacaaacctt
     6421 gcaacggtcg ccaccttcgg gctcctcctc tagtccccta actagttttc ggtattaatt
     6481 gggttcgtag ggagcttcta ggtactgaac cgtatttcct gtccgctcat taaaatcact
     6541 aacaaatttc ttgatgacat agacccgctt atggtattct accttgggat ggatattaga
     6601 attggcaaaa tagatggtca catctgcata cttggtcaaa tattctagtg tataggtact
     6661 acaaggggca cagcaaacat gcacgagaat ggttggccgc tgctcatttt tctcccatgc
     6721 ttgtaccatt ttctgcatga cacggtcata attaatcttc tgattggggg gcatcttgca
     6781 cagaatttct tctacatcga tcatgttctt ctcctttttc tagtcttatt ttatcatata
     6841 agttaggaga gctcaaatca atttagaagt gaatattata aaaagaaggg ttagtcattc
     6901 cctccttttg tgttttttat agaaagctta catcatcccg cccatcatgc ttggatccat
     6961 tgctggagct ggggctactg gttctggttt attggctacg actgcttctg ttgtcaaaat
     7021 caagctggct acagatgctg cattttgtag ggctgaacga ctcactttaa ctggatcaat
     7081 gataccttga tcaatcatgt taacccactc gccagttgct gcgttgaatc ctataccaag
     7141 ctcagcattt ttcaaacgat cgataacgat agatccttca aatcctgcat tgtgagcaat
     7201 ttgacgaacg ggttcttcca aagcacggag aacaatatta cgtcctgttg cttcatctcc
     7261 tgtcaattcc aaggtagcaa cagctggaat cacattggca agagctgttc caccacctgc
     7321 aacaatacct tcttcaacag ctgcacgagt agcgttgagg gcatcttcaa tgcggagttt
     7381 catttctttc aactcagttt cagttgcggc tccgacctta ataaccgcta caccacctga
     7441 caatttggcc aagcgttctt gcaatttttc acggtcaaat tcagaagttg tagtttcgat
     7501 ttgagacttg ataaccgcaa cacggttaga aatcgcttca ggatttcctg caccttctac
     7561 aataaccgtg ctatctttgt ccacggtcac tctcgctgct tgaccaagag cttcaattgt
     7621 cgcatctttc aactcaagac caaggtcttc tgtgataact gttccgcctg ttaagatggc
     7681 gatatcttca agcatggctt tgcgacggtc accaaaacca ggtgccttga ctgctactac
     7741 gttgaaggtt ccacgaatct tgttcaaaac aagagttgga agagcctcac catccacatc
     7801 atccgcaata atcaagagtg gacgattgct ttggagaatg ctttccaaaa gtggcaagat
     7861 ttcttggata ttggaaattt tcttgtctgt aatcaaaatg tacggatttt caaggtcagc
     7921 caccattttt tcgctatctg tcaccatgta ctgtgaaagg taaccacggt caaactgcat
     7981 tccttctacg acttcaagct ctgtttccat accacgtgac tcttcgatgg tgatgacacc
     8041 gtctttgcca actttttcca ttgcttcaga gatgtactca ccaacttttt cagaacgaga
     8101 agatacggct gcaacttgag cgatagcttc tttattggca acagggatgg cgttgttttt
     8161 caaagcttca actgctgcgg caactgctgt ttcaatccca cgacgaatac cgattggatt
     8221 tgcacctgct gtgacgtttt tgattccttc acggacgatt gcttgggtca aaacagttgc
     8281 agttgtagtt ccatcacctg cgatatcatt ggtttttgaa gctacttctg ataccaattt
     8341 ggcacccata ttttcaaaat ggtcttctaa ttcaatttct ttggcaatag tcacaccgtc
     8401 attggtaatc aatggtgagc caaatgattt ttcaagaacg acattgcgac cttttggtcc
     8461 caaagttact ttaacagtat ctgcaaggat atcgacacca cggaccatag ctgaacgggc
     8521 atctgatgaa aatttaattt cttttgacat acttactttc tccttctatt cttcaatgat
     8581 tgccaagatg ttagcttcgc ctacgatgat atacttttca tcgccatctt tgacatcaag
     8641 acctgcgtgg gcttcaacta agacacggtc tccagtttta acacttggag caaccaagtc
     8701 accgttcaag gtacgaacac cttgtccagt agccacaact tgggctgttt tggttttttc
     8761 ttgggctgaa cctgcgagaa caaagcctcc aacagtttgt tctttttctt ctacttttaa
     8821 gaccacacgg tcccctaatg gtttcaacat ctgatttcct ccataatgag atagatatta
     8881 ttagcactct ttatacctga gtgctaattc atagttctat tgtatcactt agtcagaaat
     8941 aatcaagaaa aaagtctgac tttctcaaga taaaaagcct gagaccaact cagacttttt
     9001 aatgtttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgctcaa
     9061 agcattgctt tgaggttgtg aataaagcta acgtggtttg aagagatttt cgaagagtat
     9121 taaaatggca attcttcctc ttccaagacc aaatctgcca aatcttggcc tgcattattt
     9181 tcacgcatgg cacgttgagc acgactttct aagagttgga atcctgtcac aaggacttcg
     9241 gttacatagt tcatctgacc atttttctca aagcgacggg tacgcaattc tccatcaacg
     9301 gaaatgagac tacctttggt tgcgtagctt gccaaagttt ctgctagtct gccccatagg
     9361 accatattga caaaatcagc ttcacgttca ccgttttggt ctttgtaacg acggttgaca
     9421 gcgatagttg ctcgcgctac cgacttgtca ttgttggttt tgtgcaattc tggtgtagac
     9481 gttaaacgcc caatcatgat aactttatta tacatatttt cttcctccta cttatctatt
     9541 cgtaggaaat caaaaaagtt acagaaattt gtaacttttc gaggaaattt cttatttttt
     9601 atgaatcatg aaacctgtcg cctgttgatt ggccataatg gtcatatctg taatctgaac
     9661 acggcgaggc tgactggtca catagactac tgtgtctgca atatcctgag cttgcaaggc
     9721 ttctattcct tggtaaacgg acgcagcccg ctctttatca ccatgaaaac gaacagttga
     9781 gaaatctgtt tcgacaatcc caggctgaat ggttgtcacc ttgatatccg ttgcgatggt
     9841 atcaattcgc agtccatccg aaaaggtctt aaccgctgcc ttggtagctg agtaaacagc
     9901 ggcaccagca taggcgtaga ttcctgcggt tgaccccata ttgataatat gaccttgatt
     9961 gacttttatc attgctggca agaaacagcg agtaaccgcc atcagacctt taacattggt
    10021 atccaacatg gtcaacatat ccaactcctc atagtcttga tagggagcca agtcaagagc
    10081 cagtccggcg ttgttgacca agatatcaat cctccctatc gtttctagaa tatcagagca
    10141 aacagtcttc accatgacca tatccgtcac atctagtgga aaagtccaga cggtttgatt
    10201 tggaaagctt actgcaaact ccgacttgag gatttctaat ctgtctatcc gtcgtcctgt
    10261 tagaacgaca tcctcaccct gctccagata agcacgcgca atcgcttcac cgattcctga
    10321 ggttgctccc gtaatcacta cattttttgc catcttattt ccttctatca gatgctgaca
    10381 agttcctagg cagtccagtg tttcgctggg tcgaacggtg ttccgacaac ttggtcttct
    10441 gataattcaa gcaccccacg tttttgcgga gcatttggta aatgcaattc acgaggacta
    10501 cacatcatcc caaaactctt ttcaccacga agttcgcctg ggaaaatgaa attccctttt
    10561 ggcatcatgg caccaggaag agccacaatg gttttcaacc cgacacgcgc attgggagct
    10621 cctgcaacga tttgtactgt cttgtcactt gcgactgcaa cttggcagat gttgaggtgg
    10681 tcactatctg gatgggctac catctcaaca atttcaccaa caacaaactt aggttccttg
    10741 tcattgacaa tttcttctgt aaaaccttcc gcctgtaatt cttggttcaa acaagcgact
    10801 tgctcatctg ataaaaagac ttgacctcgc tctgcaattt caaacaagct cgaaacctca
    10861 aaaatgttcc aagctactgt ttccccattt tctttgagga aaacacgggc taccttgcct
    10921 ttgcgttcca cattcagttt ggcatctccg ctatttttca cgatgaccat aaggacatca
    10981 ccgacatgtt ctttgttata tgtaaaaatc attgtttcct ttttctccta tttcaatcct
    11041 gctaaaaaat cgttaatttg ctccttactt ttacggtcgc gattgacaaa acgaccgatt
    11101 tccttgtcct tttctagaac aacaaggcta ggaattccgt aaacatccca gagtttggcc
    11161 aaatccatat actgatctcg gtccattcga ataaaggtga actctggatt ggtctcctca
    11221 atctctggta aggcaggata aatataacgg caatcgccac accagtctgc cacaaaaagg
    11281 aagaccttct tgcccgcttt ttccactaaa gatgctaatt cttctaaact tgctggctgt
    11341 atcataagac ttcctcctca tagactaggt cttcattttc atagacaaag gtataatgac
    11401 ggccatcctc aaaaatgacg ccaccaagca agctctccag actgctttcg taaacttgaa
    11461 catagagggt cgcaatttcc cccatgtcgg aaaaatggtc tcgcacaatc tctgtcaact
    11521 cttcctgagt cttcatgagc ttacggtcat ctgcaacttt tttcgtagca agagcaaggc
    11581 ttccgatacc tagcagagcc aagcctgcca tccacatttt tttagctttc ataccattca
    11641 ttttaacaca aaaaaggctt caggacaaat gaggaagcag cagaaaagca agtaaaaagc
    11701 ctcttccttt aaggaaaagg acttcttata ctaagagcaa actaggaagc tagccgcagg
    11761 ctgctcaaag cactgctttg aggttgtaga tagaactgac gaagtcagct caaaacactg
    11821 ttttgaggtt gtggatgaag ctgacgtggt ttgaagagat tttcaaagag tattattctt
    11881 attgccaggc acctaagttg ccaacgtagt aaccatcagg tgtgtaggta ttgcgaagca
    11941 tcttacctga tgaagccaga taatactact tgccattgtc tttgacccaa tcattcgcaa
    12001 tcatggaacc agaagaactt acataatacc attctccctt gtcataaacc caagtactga
    12061 ctttcatggt tcctgagcaa ttaaaggcaa aaaaactgtc caatatcatt cgttttttaa
    12121 aagcatttga cactacatca tctacgctgt agtacataga tacatcaaac gtaaaagcca
    12181 aaatatcgaa aaatatacaa cttttaaagg cagcatttca ttttttaaag tctaacaacc
    12241 atagaactga aatcagagca aggcatcaat ttcctactct tagtacgtct attttgatgg
    12301 ttgaagtgaa gattttttta tttaacgtag acaatttcca acttcccaaa agccacatct
    12361 ccacggatag tcaaggtttt gctggttggg gctacaggag cgtcagcctt agctgcacca
    12421 aaggaggttt ctactttcaa atctacacgc cagtgttgtg gaacatagac ggttgcattt
    12481 ccgaaggcca cttcaatatt taaagttgca aaatcaccta gcatctctgc attatcatag
    12541 tagattttag catccccaaa agcgacttcc acttggtcat ctacgagatc ttgatcttgc
    12601 ttatagaagg tcccgctacc aaaagcgact tccttatccg tgacgactgt ttttttccca
    12661 ttgtaccacc attttttttc attccagaac ttacttgaat gcgtcagata accaacacca
    12721 agtaccacca agatgctagc ccaaataaga gaatgattgg taactggtaa caagtcataa
    12781 gcgtaatttg caatgatgat cgccagtaaa cctgtaaaaa ctgccgaagt gagatgacgt
    12841 ctaaggatgg actcaatgga cttataagca aaaaaaacaa tacctagtaa aggccatatt
    12901 ttaccatcca aagaaggaat tccaaaattc ccttgcagca agatccaagc tgctaaaacc
    12961 aataaaacaa taccaaatgc tttctttttc atgtttttta cctcatgttt cttagatttt
    13021 cttttaggag ggattggtaa tgccgtgaca catgaacctc cttgtgggtt tgataaaagg
    13081 aaatggtgcc cgttcctgaa aaggacttgt ccaccgaata gatttgccga atattaacga
    13141 tcgttgactt ggatactcga ctaaaatagc gaggaaggat agcctccaac tcatagagct
    13201 tgaggcgaac ctcataagct tccttctggt tatgagcgta gatcttgctc ccttctgttt
    13261 caaagaagag aatttctgac aggtctagat aatattcacc tgtccccttg taaaaagtca
    13321 acctcggagc ctttgactct tgcaagagcc gttgcaagtc tgcaatttca tctgtcaaag
    13381 tgggggtctt gatgacaatt tcagtctcct ctaaattgcc gtcaatctca attcgtaact
    13441 tcatcacatc tcctttctga ctctactata tcaaagctaa gttaggaata caagggcaaa
    13501 aaagcaagtg gtcaatttga agcagtaagt ggttgtagga ctagcttaac ttacatgtca
    13561 gaataggaag aaaaggaatc cccacaccaa accacaagca tagccaacca agacatcctt
    13621 gggataatga acacccccta aaaccctcac caaccccagc aaagctgaaa agatcaagca
    13681 tactaagcca ataggtagac tagcatgcag acaggccatg gagatgatag ttgccgaaaa
    13741 gacatggcga ctgggcatcg accgtcccga actatcctta tcaagaaggg gacagatatc
    13801 ccaagtttca taaggtctcg ggtgattgat tttcttacga aacagggaca aggtcacaaa
    13861 acctgatgca ggaataagca cataaacccc aacttgctga ccaagtccta gttgcaggta
    13921 agtggtgact agcaagatca gatagaccag gggcatggcg actgtcatca aacgattgaa
    13981 actgcgtaat aggaataaaa gaattggacg actagttagt ctggagctga tatttcgata
    14041 ccattcttga taatttttca tttatgcttc tcatactttt ctagaacttg ttgagatgtt
    14101 ttccccttgg caagccaatc cactagcttg tccaagcgac gtatttcctg catcagagga
    14161 tcatcaattt cctcaatacg gacaccgcaa atcttccctg taattgcaat tcgttcaggg
    14221 acataatatg gtgcttgtct aaaaaaatct ccgtaagtaa catccctatc cagacaagca
    14281 agaacatcac tcacttcata ccctgtcaac caactggtca cttggtgaac tgactctgcc
    14341 tttccaccct ttcgctcaac ttttgcaatc aaggctaagt agacagcagc aaatttcata
    14401 ttgtataatt tctgactcat acttctctct ttctatgtgt actagtataa ataagaaaaa
    14461 gaaggccgtc aagccttctt ttgatttatt cttctgcttc atcttctgta aattgactat
    14521 tgtacaagtc agcgtagaag ccaccttgcg ccatcagttc ctcatagttg ccttgctcga
    14581 tgatatttcc atctttcatg accaagatca agtctgcatt tcggatggtt gacaagcggt
    14641 gggcaatgac aaaggatgtg cgtcattcca tcaaacggtc catggctttt tggatcaatt
    14701 cctctgtccg tgtgtcaaca gaagaagtcg cctcatccaa aatcaaaagc ggtgcatcct
    14761 taagaagggc acgagcaata gtcaatagtt gtttttgtcc tacagacaag gtcacggtgt
    14821 catccaagat ggtatcatag ccatctggca aggtcataat aaagtggtga attcccacag
    14881 ccttactagc ttccatcatt cgttcatcac taatccctat ttgattatag atgagattgt
    14941 ctcgaatagt tccttcaaag agccaggtat cctgcaagac cattgaaaag gcatcatgca
    15001 cttctgaacg cgtcatagcc ttggtatcca caccatcaat gcgaatactt cccttatcaa
    15061 tctcatagaa tttcatcaaa agattgacaa tggttgtctt accagcccca gtcggcccaa
    15121 caatggcaac cttttgacca gcatgagctg tcgcagagaa gtcatagtct tgaacattga
    15181 caccgtccac cagaatttct cctgctgaca cgtcgtagaa acgtggaatc agattgacca
    15241 gagttgattt accagaacct gttgacccaa taaaggccac tgtttgacca gtttctgctt
    15301 taaagctaac atgttcaata actgcctccg aatttgccgc atagcggaag gtcacatcct
    15361 taaactcgac ctgacctttg aagttttcat cagtcagctg cacttgaaca gggttttgga
    15421 tagaagaatg caaatctaaa acttgattaa tccgcttagc agagaccata gttcggggaa
    15481 gaacgatgaa gagtgctccc atgagaagga agcccatgac aacctacatg gcataagaca
    15541 tgaaaacaat catgtcacta aagagaggca gacgcgctat cggagcagcg tcgttaatca
    15601 cataggcccc aatccagtaa atcgctacac tcaaaccact tgaaatcccc atcatgatag
    15661 gattcaaaat agccataaga cggttgacaa acaaattcaa acgggtcaat tcatcattta
    15721 ctgctgcaaa tttttcattt tgataatcct ctgcattgta ggcacgaacg acacgaatac
    15781 ctgttaaact ctcacgagtg atactgttca gtttatctat cagcccctga atcaaggact
    15841 gttttggaaa gtctagcgtc atcaaaacgg tcgtcatcag gacgttgaca atcactgcca
    15901 caagtacggc ccaaagccag tattctgaat gacctaaaat cttcccaata gcccaaatag
    15961 ccataattgg accacgcgtt accacttgca agcccatagt aatcaacatt tgaacttgag
    16021 taatgtcatt ggtagtacgc gtcaagaggc taggaattga aaatttctta atctctgtct
    16081 gcgagtaatc caaaactcgg ttaaaaatat cacttctcag cctactagta taagaagccg
    16141 ccactcggga tgcaaaaaat ccaactgcaa ctacggacaa gaaggcaaga aagagcattc
    16201 ccatcatcat gcttgccggc tgccacaact catctaaatt agttccttga ctacctagca
    16261 aatccgtaat tttcgagata taggtcggca cttccaactc tagatagacc gaaaagcaag
    16321 taaagagaat ggctagtaaa atcatcccca ttcttttcta ctaattcttt tggctaattt
    16381 ctttattctc tcctcctatt cccttgatat tttgcctgta gttgaccgag aactttctca
    16441 aaaatcagta attcatcttc atcaatgtct tccatcaact gcttgtctat gcgttcaaaa
    16501 aaagccttaa cctgttgcat ctgagaacgt gctttgtccg tcagacgaac aaacttagcc
    16561 cgcttatcaa caggactcgc ctccaattcc accaaaccat tttgcactat acgcttaacc
    16621 agattactag caacagactt ggtaatattg agttcctgct cgatatcttt aatcaagacc
    16681 aagtcttggt ttttctcgcg attatccaaa aaacgcacaa cctgaccttg cggcccaccc
    16741 ataaattcaa tgccgcaacg tttggcttcc ttttgcacca tcaggtaaat ttgatgacca
    16801 aaacgcttaa agactaacat cggtttatcc ataatctccc ccttctaaat aaaaatagtt
    16861 ctctggagaa taattaaatt tctatgagaa ctattttctt gattaaaaaa atcccaagtg
    16921 attttctcac ttaggatcat gttctatagg ttaaattaaa acccatctac gttcgtataa
    16981 atcttttgga cgtcttcgtc gtcttcaaga acgctgtaaa gtttttcaaa ggtttcaagg
    17041 tcttcgcctg acaattccac ttctgactga ggaatcattt ccaattcagt cacttggaat
    17101 tcttcaatac cagactcacg gagagcaacg atagccttgt gaaggtcagt tggcgctgtg
    17161 taaactgtga ttgtaccttc ttgtgcttct acgtcatcca catccacatc cgcttcgagc
    17221 aattgctcaa agactgcgtc cgcatcttca cctccaaata caataacacc tttgttgtca
    17281 aagaggtaag aaacagaacc tgaagcgccc atgtttccgc cgtttttacc aaaggctgca
    17341 cggacattgg ctgctgtacg gttgacgtta gaagtcaaag tatccacaat tagcatagag
    17401 ccatttggcc caaaaccttc gtaacgtcct tctgtaaagg tttcgtctgt gtttcctttg
    17461 gctttatcaa tcgctttatc gataatgtgt tttggcactt gggcttgttt agcacggtcg
    17521 ataacgaatt tcaaagctga gtttgattct ggatctggat cacctttttt agctgctaca
    17581 tagatttcta caccaaattt tgcatatact ttagagttgg ctccatcttt agccgttttc
    17641 ttggctacga tattggccca tttacgtccc attaggaatc tccttttttc acattttaat
    17701 ctttcttatt ataacacaag tttttttgat tttcactaga ggaaatggat tttattagca
    17761 aatcaagcta ggatagcact ttacctgcta agatggtctt acctttctat ctttatcaac
    17821 agacactcat ccacattcaa aaaacaaact agaccattat ctgcaaatag aaagtttcag
    17881 ccaagtttga caaagtcagc tcaaattact gtttgaagtt tgtagatata agcgacaaaa
    17941 acaatcatac tgcacctttt gttgacagtc tactccagac atatcatagt tcaagtaaat
    18001 actttgaaat ccaacagttc ttataagcgc tattgtattc taagaaatca atagaagagt
    18061 ttctaagcaa acctctaata ctcaataaaa atcaaagagc aaactagaaa gctagccgca
    18121 ggttgctcaa aacactgttt tgaggttgcg gatagggctg acatggtttg aagagatttt
    18181 cgaagagtat aatttacgtg ttcctaagat ggagaagtta gactagtaca ctggcacttc
    18241 taaaccattg ctagcaattg atttgttcat atttaatttc attttttcca taaatgggta
    18301 ttagatataa acagcaaaat atttccgata cgtgtcattc ttgaatttcc aatcatctaa
    18361 aacaagtaaa ggataatcaa tcccctgtat atcaaggaat tggctaccct ttttactttt
    18421 ttacacattc tgtttgatag attcatttta acatcacgag catactccaa tggaaatcgc
    18481 taggcaagag ataaactttc agatatccgc agagagatca tcgcctcttt ttgtcgcaag
    18541 cattctcctc tcctagtcat tttctacctt atcttctacc tgaggataga gagttgttcc
    18601 ccaaatagaa atcgtccgct tacgcactag tggcaaatcg gttttttcat aaaccgtacg
    18661 ccaccattcc caggcaagcc cggtacactc tctaattttg acagagagat tacgaacatt
    18721 cccttttaaa ggaatactag tggtaaagtg agccgttaaa tcctgcccat ttctgtccca
    18781 agccttagga gtcaagactt ccttaccttg atgatcatag gataattcat tccaagtaat
    18841 ataatattgg gcaacatagg caccactatg atccagcagt aaatctccgt ttctgtaagc
    18901 tgtaacctta gtctcaacat agtctgtact attttgaaag gtcgcaacta cattgtcacg
    18961 taaaaaagaa gttgtatagg aaatcggcaa gcctggatga tctgctgtaa agcgactgcc
    19021 ttcttgaatc aagtcctcta ccatatccac cttgcctgtt acaactcggg cacccgaact
    19081 tgggtcgccc cctaaaataa ccgccttcac ttctgtattg tccaaaatct gtttccactc
    19141 tgtctgagga gctaccttga ctccttttat caaagcttca aaagcagcct ctacttcatc
    19201 actcttactc gtggtttcca acttgagata gacttggcgc ccataagcaa cactcgaaat
    19261 atagaccaaa ggacgctctg cagaaattcc tctctgtttt aaatcctcta ccgttacagt
    19321 atcttgaaac acatctcctg gatttttaac agcatctaca ctgactgtat aataaatctg
    19381 cttaaaatta acaatctgaa tctgcttttc gcctgaatgg acagagttaa aatcaatatc
    19441 aagagaattc cctgtctttt caaagtcaga accaaacttg accttgagtt gttccatgct
    19501 gtgagccgtg attttttcat actgcattct agctgggaca ttattgacct gaccataatc
    19561 ttgatgccac ttagccaaca aatcgtttac cgctccgcga acacttgaat tgctggggtc
    19621 ttccacttgg agaaagctat cgctacttgc caaaccaggc aaatcaatac tataagtcat
    19681 cggagcacga tcgaccgcaa gaagagtagg attattctct aacaaggtct catccactac
    19741 gagaagtgct ccaggataga ggcgactgtc gttggtagct gttacagaaa tatcacttgt
    19801 atttgtcgac aagctccgct tctttctttc gataacaaca aactcatcgg gtagctgatt
    19861 accctctttg atgaaacgat tttcaatact ttctccctga tgggtcaaga gtttcttttt
    19921 atcgtaattc atagctagta taaagtcatt tactgcttta tttgccatct tctacctcct
    19981 aataagttcc tggattgagt tgcataaact cagacttgtt cagcgaaatc agccgtggtt
    20041 ggactaagtg atccaaaatt tcctcgtaca attcttctga gacattgcgt cgccgtctgg
    20101 ctagataaga agtcggaata accgtattat ccaacataaa taccttatct aagtcaatca
    20161 aggttggtct tgtaaaagga ttacgagcta gatccggctc ttctatcata aagttcttga
    20221 ccaaacgtct ggtcaagaga gctggtttga aggtctgatt tttaaccaac tctttgtttt
    20281 tagtcatgct gttgtcaata cagatataca tatgattctt cacagccaaa tcgctactaa
    20341 tagtcggaaa aggcaaataa agagctacaa catctcctct cttaatcaag caagagcacc
    20401 cccttttctc ctaatgtaac atagacagga ttgaccaagt cttctgattg actcagaatt
    20461 tccaaagttt gagtttggcg cgctgtcaat ttagtagcat cttgtctctt caatacaaaa
    20521 tgcttgtcgc caataacctt gacaatataa tccttctcca aagctgactg gtaaatccac
    20581 atcagatgtt gtctgtcctg agaactcaag agagaaggat tttcaagcct cccgatagtc
    20641 tgataaaaat caaaaacagg agctaactcc tgccaatctg attggctagt tgtcaaggct
    20701 agaaaaaggg ctttgcgagc tgatacttct tggttagcct tgagagttac tttcccctcc
    20761 aagtttttta gaaatcggga aactccagaa agcaaatttt tctctaactg cgagaaataa
    20821 aaacctttcg ttcccagaca taagtctttc atgtcgcttt ctctagcaaa taagagctca
    20881 aacatttgat agtaaaagaa aaatatctgg cactgggtcg cgctcatctt ttccttatcg
    20941 gcttcttttt ttaaccagag caagggcgac aggtagctgg attgagacat ttcctctacc
    21001 tcctactctt ttttaactgg agcatctgca ctagctgcca cttcttttga ctggatactt
    21061 tcccactggt taatctcctc tgagataaga ccttcgcatg tcttgacaaa tagggcaaaa
    21121 gccttggtct ttcctgcata tttctccgtt tggcattgat agaggaattt ttctttctcc
    21181 aggagttgcg cagttttttg gtaagaaatc caattttcct ttgcattata caaattgata
    21241 atcccctcac acagcaagcc gagactggat aaggcaaccg aaatcaaacg gtagcgatca
    21301 cctggcatag gaatagcaca aaagacagct atgaggaaac ctgccacgat ttctgttatt
    21361 tttaatacct tatagcgcct acgatgttga acgcttttct ttaaaaaatg agctatctgt
    21421 acgtctaatc gctctgtcag gtacatttct tctggcgtca tattcgtaac tcctttcatt
    21481 tactttgata accatagtat aacatatttg aatatcaaag tatagtaaaa actggactat
    21541 aacttattag actccctgcg aaacaaaata tggtatagta gttctatgaa ttatgaagca
    21601 agtaaacaat taactgatac acgatttaag ctccttgttg gtgttcagcg cacgactttt
    21661 aaagagatgt tagctgtatt aaaaacagct tatcaaaaaa gtcggacaag ctggtaaaat
    21721 cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac aaacttcacg
    21781 taaatccagc aaactcaaac cgctaacagt tgaagataaa gcctgtaacc atgcgctatc
    21841 taaggagaga agcaaggttg agaacatctt tgccaaagta aaaacgttta aaatgttttc
    21901 aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga ttgctggtat
    21961 tatcaatcat aaactaggat tctagttttg caggaagtct attataaaaa ttgaagaaat
    22021 taaaaagaga tgatattagg gagaactgat ttaaaagttc tccctattta acattattaa
    22081 ataaaaccta attatatagt aaactgaaat aagatataaa caaatgaatt ggagcttaac
    22141 atccatttcc agcaattttt tagaaactac agtgaactac tctggattca acacattata
    22201 attgaaattg aaggttagat ttaaagtaat aaagaataat attacgctct aaaattagat
    22261 aaatttcaaa taattcaaat aattaaggat acctatcact attataaagt aactgaacaa
    22321 cattttatat ttaacaatta tttaaaataa taaaataata acattatata aattatttta
    22381 aaaaataatg aaacgttcca acttatctat tgacacgttc caagaaatcg gatacaatgt
    22441 aattaacaat aaagatagga gaaaataatt tgaatccatt aaaagaatgc tatgaaaaaa
    22501 acggtatact aattgcagca caccgtggta caaacggagg aaatatatta caaaacacat
    22561 caaaagccta tttgaattcc atacgccata acgcagatat gtttgaggtt gatgtaattc
    22621 gttcaaaaga tggagaattt tttgcatttc acgatgggca agaaccttta gtatttggtg
    22681 aaaaaattga tattagaact ttgagttctg aagagatcca aagaaaggaa tgttttaatt
    22741 cactgaatga acccatatta caaaagattg aaaaaatttc tgatgtatta aaaaattttg
    22801 agggacagtc tttaataaat atagatagaa gttggtttta ctgggatacc ttcctagata
    22861 agttaaaaat atatccatct ttgaatcaag ttcttttaaa atcacctgta aaaaaagaat
    22921 tattggaaat gcttagagac tctggtttga atgttatgta tatgccaatt atcaaaacaa
    22981 ttgaagagtt agattttgtt ctctcatttg aaggaataaa tacagtagct gtcgaacttg
    23041 tattccaaga tttagaaaat ccaatcataa gtaagaaaat aattgatgat ttacatgaaa
    23101 aaggattact tatttgggtt aatgctttaa ccttaagtga ttcaattatt ctttcggcta
    23161 agattgatga tgatacagct attgctcatg atggcgaatc ttgggggaaa ttggtttcaa
    23221 taggatttga tattattcaa acagattggc cattattact ttatcaatat ctagttgaaa
    23281 aaaataaaaa aataaaaaaa taaaaaaata aaaataaagg agaactatca tgttcaaaaa
    23341 ctttaaaaag attgcattca cttcttcact aattttactt gctgcttgct caaacaatgc
    23401 ttcaaataca aatcagtcta ctgatgaagg aagtgttgat aaatctcaag aaattgtaat
    23461 ctatacaaat tcggcttcta atggacgtgc agattggtta caaactaaag catcagagca
    23521 aggatacaat cttcgtttgg tagatatttc aggtggagaa ctagctgacc gtttgattgc
    23581 agaaaagaat aatgctgtgg ctgatatggt aattggatta aataaattag aatttaaccg
    23641 tattaaagct gaaaaacttc ttgtaaaata ctcaccaaaa tgggctgatg aagtagatag
    23701 ctctttggga gattctgaag ggtattattc accaattgta aatcaaccat tagttttgat
    23761 tggtaataaa gatgctaaaa tgccatccga ctggacagat tttactgatt catcttataa
    23821 aggtaaatat ggtatttcaa aattaagtac aggtacatct aagaacatct ttgcaagtat
    23881 tgtttcgaga tataaagatg aaaaaggtga gttaggaata tctgatgaag gttggaaagt
    23941 tgcaaaagct taccttaaaa atgcacacgt ttatgcagaa ggtgaagact atatctcttc
    24001 aattatggat aataataatg atctaaaata tagtatgatg tggggatctg gtgttctaca
    24061 aaatcaaaaa gaacgtgaat ataaattcca agtaatgtca ccaaaagttg gtgttcctta
    24121 tgtaactgaa caagttggga ttttaaatac aagtaagaag caagcatt
//
LOCUS       DAVDWM010000032        23793 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVDWM010000032 DAVDWM010000000
VERSION     DAVDWM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.032"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..339
                     /locus_tag="VUW49_001450"
     CDS             <1..339
                     /locus_tag="VUW49_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET7981351.1"
                     /translation="KPSEEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEESPEESE
                     EPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAE
                     KLLALLKESK"
     gene            481..855
                     /locus_tag="VUW49_001451"
     CDS             481..855
                     /locus_tag="VUW49_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981352.1"
                     /translation="MTTSYLKSKSDNGGGTYCLDNIELLANIDFIKIFEEISTSSKID
                     IVTYSFYDDEFLKALLYDKINVRVLLSKVSNQSQHYTDLIREIVPNSEVIQVEETHAK
                     IVLAEPNFVYLGSQNIDQSDWF"
     gene            943..1143
                     /locus_tag="VUW49_001452"
                     /pseudo
     CDS             943..1143
                     /locus_tag="VUW49_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1308..2246
                     /locus_tag="VUW49_001453"
     CDS             1308..2246
                     /locus_tag="VUW49_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HET7981353.1"
                     /translation="MVIKKYFVNSIESYIQTIREINSANPSHRLWYRGQKSSEYGLIP
                     GIWREAYSVSDSRGERIPIHRTNFYNPKGEVVVMPSQNVLLEKFMSKAMTEDILGPKN
                     EVQWLELAQHYGLPTNLLDWSTDPLVALFFAASTVDCKNDNETYLTEDQVIVREDDED
                     EDEIIHAHASVWVINSLEINRILLNEPGIEGVFQSFNPNDIEKINKEISLNCGTFCFE
                     GSKINPRITRQSGNFTHSGTKLTHPLDFLAAVQSKIIKINIPYSCISKIITDLNKLDI
                     TNESIYFGRTERDLTAERIKGEVYSEFKEYLNSIIE"
     gene            2307..3020
                     /locus_tag="VUW49_001454"
     CDS             2307..3020
                     /locus_tag="VUW49_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET7981354.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAENVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            3078..3680
                     /locus_tag="VUW49_001455"
     CDS             3078..3680
                     /locus_tag="VUW49_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981355.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGKRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3816..4469
                     /locus_tag="VUW49_001456"
     CDS             3816..4469
                     /locus_tag="VUW49_001456"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7981356.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            4479..4943
                     /locus_tag="VUW49_001457"
     CDS             4479..4943
                     /locus_tag="VUW49_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET7981357.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGM
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4956..6224
                     /locus_tag="VUW49_001458"
     CDS             4956..6224
                     /locus_tag="VUW49_001458"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7981358.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            6238..6498
                     /locus_tag="VUW49_001459"
     CDS             6238..6498
                     /locus_tag="VUW49_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981359.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            6509..7870
                     /gene="pdrM"
                     /locus_tag="VUW49_001460"
     CDS             6509..7870
                     /gene="pdrM"
                     /locus_tag="VUW49_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET7981360.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGIPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            8024..8992
                     /locus_tag="VUW49_001461"
     CDS             8024..8992
                     /locus_tag="VUW49_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7981361.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            9008..10000
                     /locus_tag="VUW49_001462"
     CDS             9008..10000
                     /locus_tag="VUW49_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7981362.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            10218..11261
                     /locus_tag="VUW49_001463"
     CDS             10218..11261
                     /locus_tag="VUW49_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET7981363.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     NTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            11325..13010
                     /gene="lpdA"
                     /locus_tag="VUW49_001464"
     CDS             11325..13010
                     /gene="lpdA"
                     /locus_tag="VUW49_001464"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7981364.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            13074..14063
                     /locus_tag="VUW49_001465"
     CDS             13074..14063
                     /locus_tag="VUW49_001465"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET7981365.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(14136..15206)
                     /gene="xerS"
                     /locus_tag="VUW49_001466"
     CDS             complement(14136..15206)
                     /gene="xerS"
                     /locus_tag="VUW49_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET7981366.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETAGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(15790..17340)
                     /locus_tag="VUW49_001467"
     CDS             complement(15790..17340)
                     /locus_tag="VUW49_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7981367.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(17356..18135)
                     /locus_tag="VUW49_001468"
     CDS             complement(17356..18135)
                     /locus_tag="VUW49_001468"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7981368.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(18122..18973)
                     /gene="ylqF"
                     /locus_tag="VUW49_001469"
     CDS             complement(18122..18973)
                     /gene="ylqF"
                     /locus_tag="VUW49_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET7981369.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(19244..>23793)
                     /locus_tag="VUW49_001470"
     CDS             complement(19244..>23793)
                     /locus_tag="VUW49_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET7981370.1"
                     /translation="DENGNLIEPPVNDIPEYTESVGTTGVDENGNLIEPPVNDIPEYT
                     EPISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQI
                     VTSYEELHGKKISEPVETVTILKEMKPKILVKGTKEKTVPVLTLTKVTEDAMNRSANL
                     NYELDNKDNAEISSIIAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMIYD
                     RGQGSETSKLDEKTLRLELKKVEIKNISSTNLVKVNDDGTEIPSDFMSEKPSDEDVKK
                     MYLKITSRDNKVTRLAVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEYIHYIE
                     KPVPKVNNVYYNFNELVRDMQEHPNDEFKLGADLNATNVSAFGKSYVTKDFKGKLLSD
                     GDNHYTIHNLSRPLFGNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTTIENVK
                     VKGNIVGKDWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERA
                     YVNANLIGKKAKAAGIAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESGGVVGS
                     MKHHGSVEDSVSMMKVPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGHSSYQR
                     SKDKNRIRPISEEEAKSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQDYKVD
                     RDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDH
                     VMIHYADKTKEIKAVHQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKDIKDRL
                     ATVELISPEVRALMDKRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDKLVKSL
                     IENADHQLNSDEAAMKALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLMMFKPD
                     FYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTND
                     TDLNDWFIHAAKNVYIVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMF
                     LISTYNTMAYSSFEKYGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSVRDQLL
                     KSQNRVPTPVWDNQNYHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWYMGAMA
                     SIFQDPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQ
                     GMLQTPDKSGNGEYGALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNYNDALM
                     MLDHLEADAVIPKLHGNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIVINNID
                     DLVNNNLMTKHGAPSDRTYNPEDFDSAYVNINMMTGIYGGNTSQGAPGAASFKHNTFR
                     MWGYFGYENGFISYASSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKKQYFKD
                     VKSKAEKGFTAIEIDGRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTVDLKSK
                     VFKALLKNTDGFFNKLFKEDI"
BASE COUNT         7266 a         4479 c         4683 g         7365 t
ORIGIN      
        1 aaaccaagcg aggagaaacc tcagacagaa aaacctgagg aagaaacccc tcgagaagag
       61 aaaccgcaaa gcgagaaacc agagtctcca aaaccaacag aggaaccaga agaatcacca
      121 gaggaatcag aagaacctca ggtcgagact gaaaaggttg aagaaaaact gagagaggct
      181 gaagatttac ttggaaaaat ccaggatcca attatcaagt ccaatgccaa agagactctc
      241 acaggattaa aaaataattt actatttggc acccaggaca acaatactat tatggcagaa
      301 gctgaaaaac tattggcttt attaaaggag agtaagtaaa ggtagcagca ttttctaact
      361 cctaaaaaca ggataggaga acgggaaaac gaaaaatgag agcagaatgt gagttctagt
      421 tctcattttt ttatgaatat gatagattta taaaattgaa agggaaacgg tgtaaaagtt
      481 atgacaacat cttatttaaa atctaaatct gataatggtg ggggcactta ctgtttagac
      541 aatatagaat tgttagcaaa tattgatttt atcaaaatat ttgaagaaat ttctacttct
      601 tctaaaatcg atatcgttac ttatagtttt tatgatgatg aatttttaaa agctctactc
      661 tatgataaaa tcaatgtcag agtcctatta agtaaagtct ctaatcaaag tcaacattac
      721 acagatttaa ttcgagagat tgtacctaat tcagaggtga tacaagtcga agaaactcac
      781 gctaaaatcg ttctggctga acctaatttt gtatatctag ggtcccaaaa tattgatcaa
      841 tcagactggt tctagacaag cgtagttatt agagaccaag aaatttataa atagacttcc
      901 tgcgaaacaa aaatataata caataaaact atgaattatg aatcaagtaa acttaagcca
      961 ctaactcttg aagataaatc ctataaccat gtgctatcta aagagagaat caaggttgag
     1021 aatatttttg ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcgacgt
     1081 aaacgctttg gattacgaat gaatttgatt gctggaatta tcaaccgtga actaggattt
     1141 tagtttcgca ggaagtataa taatatgagt tcgattttta atatcctatt tatttaaaac
     1201 atagaaaaat gaaaaccctt aaaaagaaag gaagaagaat tttaaaataa ttattggagt
     1261 atttttagct tttttacttt tttaagtgta aatggtataa tatacctatg gttataaaaa
     1321 aatattttgt aaattcaatt gaatcgtaca ttcaaactat tagagaaatt aattcagcga
     1381 atccatctca tagactatgg tatagagggc aaaagagttc cgaatatggc ttgattcctg
     1441 gaatttggcg tgaggcttat tctgtttccg attctagggg agagagaatc ccaattcatc
     1501 gaacaaactt ttacaaccct aaaggagaag tagttgttat gccatctcaa aatgttctcc
     1561 ttgaaaaatt tatgagtaag gcaatgactg aagatatctt agggccgaaa aacgaagtac
     1621 aatggttaga attagcacaa cattatggct taccaacaaa tttattagat tggtctacag
     1681 acccattggt tgcattattt tttgctgcct ctacagttga ttgtaaaaat gataatgaaa
     1741 cttacctaac tgaagatcaa gttattgttc gagaagatga tgaagatgag gatgagataa
     1801 ttcatgctca tgcttctgta tgggtaatta attcgttaga aataaaccga atcttactaa
     1861 acgaacctgg tatagaagga gtttttcaga gtttcaatcc taatgatatc gagaaaatta
     1921 acaaagaaat ttctttgaat tgcggcacat tttgttttga ggggtctaaa attaatcctc
     1981 gtatcaccag acaatcaggt aacttcacgc actcaggaac aaaacttacg catcctctag
     2041 attttttagc agctgttcaa tcaaaaatta taaaaataaa tataccttat agttgtattt
     2101 ccaaaattat aactgactta aataaattag atattacaaa tgaatctata tattttggac
     2161 gtactgaaag agatttgact gcagaaagaa ttaaaggcga agtttattcg gaatttaagg
     2221 aatatcttaa ttcgattatt gagtaatcat tatttgtaca cccccatttg aaccattgca
     2281 ccccctctag aaaggaaaac ctcaaattgt tctacaaatt tctactcttc gacctcgacc
     2341 acactcttct tgattttgat gctgctgaga atgtggcttt gacccaactt ctaaaagaag
     2401 aaggagttgc ggatattcag gcttataaag attattacgt tcctatgaac aaggctctct
     2461 ggaaagactt ggagctgaag aaaatcagta aacaagagct ggttaacacg cgcttttctc
     2521 gtttatttgc tcattttgga caggaaaaag acggtagttt tcttgcccag cgttaccaat
     2581 tttacctcgc ccagcaggga caaacactat cgggcgctca tgatctcttg gacagcctca
     2641 ttgagcgtga ttataacttg tatgctgcga caaatggcat tactgccatt cagacaggac
     2701 gtttggctca atctggtcta gcaccttatt tcaatcaagt ctttatctca gaacagttgc
     2761 aaactcaaaa gccggatgct cttttttatg aaaagattgg ccagcaaatt gctggattta
     2821 gtaaagaaaa gacgctgatg attggagatt ctctaaccgc cgacattcaa ggtggcaata
     2881 atgcggggat tgacactatc tggtataatc ctcatcacct cgaaaatcac acacaagccc
     2941 agccgactta cgaagtctat tcttaccaag acttgctgga ttgtttagat aaaaatattc
     3001 ttgaaaagat cacattttaa aggagacgag ctaatgacta caaaaaagct aatattacta
     3061 ttgaagagta cattgaaatg tctgaagttg attttaatga agctgttaat tatgaattta
     3121 catctgacac ttgtcaatta gcaaatagta tttatcaatc tctttttaag ttttttgata
     3181 agaaaaattt ctctggcgat ttaattttta cttggaaatc tccatcatta gtcaaagaag
     3241 gggattatat tgggaaaagg gattcacaag tagataatct tagagtaata ggaaatatat
     3301 ttccgaatta tcttactaat cgaaaatata gcctcaatat gaatcgtaat ggctgtatgg
     3361 gagattttcc tcatgacttt tttgatatat acctagatca tgtagcaaaa tatgcctacg
     3421 aacaaaaagt taataatatt aaagagtatt atcctttaaa aagagcgatt ttacaccaag
     3481 agaatgcatt gtattttcga tttttttcta attttgacga ctttttagaa aaaaattatt
     3541 taaagactat atggcaagtt tctaaagaaa ctcctttttc tgaaatggat tttaatatgt
     3601 ttaaaaatat ttcagaaaag ataatcttcg aaagaggtag taaaatgctt aatgatttaa
     3661 aaagtaatta taaaaaatag ccagtcttta taaactgtat tcacgtattc aaatcgtata
     3721 tagtcgattt attaaatctt tagataaatt gtaaaaagtg gtttccatag ccactttttg
     3781 ctataataga ggcagtaaaa atgaaggagt cagctatgga acactcgtct tggcatgctt
     3841 tgattaaggc gcaattacct gagggttatt tcgggaaaat caatcagttt atggagcagg
     3901 tctattctca ggggattatt tatccaccca aggaaaaggt ttttcaggct ctcttgacaa
     3961 cactgcttga agaagttaag gtggtaattc tagggcaaga cccctatcac ggaccaggtc
     4021 aagcgcaggg cttgagtttt tctgtacctg actctatccc agctccacca tccttgcaaa
     4081 atatcttgaa agaattgtca gatgatatcg gagtcaagaa atctcatgat ttgacagctt
     4141 gggctgagca aggagtcttg cttcttaatg cttgtttgac tgttcctgct ggacaggcca
     4201 atggtcatgc tggtcaaatc tgggagcctt ttactgatgc tgtgattcag gtggtcaatc
     4261 atctagatag accagtcgtt tttgtactct ggggagctta tgcacgtaag aagaaggcct
     4321 tagttaccaa tcctcatcac ttgattatcg aatcagccca tccaagtcct ttgtcggttt
     4381 atagaggatt ttggggttcc aagccctttt ccaaggccaa tgcattctta aaagagacag
     4441 gacaagagcc aatcgattgg cttagataag gagaaaatat gcctcagtta gcgacgattt
     4501 gctacattga taatgggaaa gaactgctca tgctccatcg taataagaaa cccaatgatg
     4561 tccatgaagg gaaatggatt ggtgtgggtg gtaagctaga gagaggagag acgccccagg
     4621 aatgcgcggc gcgtgaaatc cttgaagaaa cagggctcaa agccaagcca gttctaaaag
     4681 gtgtcatcac ttttcctgaa tttacaccag atttagactg gtacacctat gtttttaagg
     4741 tgacggagtt tgagggcgac ttgattgact gcaatgaggg gatgctagaa tgggttccct
     4801 atgatgaggt tttgagcaag ccgacttggg aaggtgacca cacctttgtt gagtggcttt
     4861 tagaggataa acccttcttt tcagccaagt ttgtttatga tggggataaa ttgttggata
     4921 cccaagttga tttctatgaa taaaggagaa agcagatgct actaatcaaa aatggtcgtg
     4981 taatggatcc taagtctggt ttggatcaag tttgtgatgt cttagttcaa gatgggaaaa
     5041 ttatcaaaat tgcgcctgag atcacggaag aaggagcaga aacgattgat gctactggtc
     5101 ttgtggttgc tcctggtttg gtcgatattc atgtgcattt ccgtgaacct ggtcaaacac
     5161 ataaagaaga cattcatact ggtgccctgg cagccgctgc aggtggtttt actactgtcg
     5221 tcatgatggc taataccagt ccaaccattt cagacgtgga gactttgcaa gcagttctcc
     5281 agtcagctgc caaagagaag attaatgtca agacagttgc gaccattact aaaaacttta
     5341 atggtaaaaa cttgactgac tttaaggcgc tcttagaagc tggtgcggtt ggtttctctg
     5401 atgacggtat tccgcttgag agcagtaaga ttgtcaagga agccatggag gaagccaaaa
     5461 aactcaatac ctttatcagc cttcatgagg aagatccagg tttgaacggt gttcttggct
     5521 ttaatgaaaa tattgctaga gaacatttcc atatctgcgg tgctactggg gtggctgagt
     5581 acgctatgat ggcgcgtgat gtcatgattg cctatgcaac taaagcccat gttcacatcc
     5641 agcatttgtc taaggaagaa agtgttaaag tagtggagtt tgctcagggg ttaggtgcag
     5701 aagtcacagc agaagtagcg ccacagcatt tctctaagac agaagcactt cttttaacac
     5761 aaggtagcaa tgctaagatg aatccaccgc ttcgtttgga atcagaccgt cgtgccgtta
     5821 tcgaaggtct caaatcaggt gtcatcacag ttattgcgac tgaccacgcg cctcatcatg
     5881 tagatgaaaa aaatgttgag gatattacca aagcgccatc tggtatgact ggcttagaaa
     5941 catccctgtc tctcggcttg acctatttag tagaagctgg tgagttgagc ttgatggaat
     6001 tacttgaaaa aatgacatac aacccagcca agctttacaa ctttgaagca ggttacttgg
     6061 ctgagaatgg tccagcagat atcactattt ttgatgccaa ggctgaccgc cttgtggact
     6121 cccattttgc ttccaaagca gctaattcac cattcatcgg tgaaacctta aaagggcagg
     6181 ttaaatatac catctgtaag ggacaaatcg tctatcaagc ttgataggag ttagaatatg
     6241 aattattttc gaaaacgtag ggagagacaa gcgaaatcta atagtggaat ttactgtccc
     6301 gcagctaatc gttccaaaat tcaatatgaa actaaagaaa aagctgacca tgcggttcaa
     6361 tatgataacg gaggtttagt tagaagctat tattgtcgta catgtgcgtg ttggcatact
     6421 acttcaaaat ctaataaacc tccgctaagt ttgaaaaact atggtctgaa actctttggt
     6481 ttagataaat cagaatagaa agagaagtat gtataagaca aagtgtttac gagagaagtt
     6541 agtattattt ttaaaaattt tcttcccaat cctgatctac caatttgcca attattctgc
     6601 ctcttttgtt gatactgcaa tgacaggtca atacaacact atggacttgg ctggtgtatc
     6661 tatggcaacc agtatctgga atcctttctt tacatttcta acagggattg tgtcagcctt
     6721 ggtgcctatc attggtcacc atcttggtcg aggcaaaaag gaagaagttg cgtctgattt
     6781 ttaccaattt atttatttgg ccttgggcct atctgtggtc ttgctgggga tggtactttt
     6841 cttggcacca acaatcttga atcatattgg gttagaagca gcagtagcgg cagtagcggt
     6901 tcgctatctt tggtttttat ctatcgggat tatccccttg ttgctcttta gcgtcattcg
     6961 ttccttgctg gattcgctgg gcttgaccaa actgtccatg tacctcatgc ttttgttact
     7021 ccctctcaat agcggattta actatctctt gatttacggt gcctttggta ttccagaact
     7081 gggaggggct ggtgctggtt taggaacatc cttggcctac tgggtcttgc ttgggatttc
     7141 tgttctggtt ttatttaaac aggagaagct caaagcctta caccttgaga aacgaattcc
     7201 acttaatatg gataacatta aggaaggagt tcgtttaggt ctgcctattg ggggaactgt
     7261 cttcgcggaa gtggctatct tttcagtggt tggcttgatt atggctaagt tttcgtcctt
     7321 gattatagct agtcaccagt cagctatgaa cttttcaagt cttatgtacg cctttcctat
     7381 gagtatctca tcggctatgg ctattgtcgt ttcctatgaa gtgggagcca agcgatttga
     7441 tgatgcgaaa acctatattg gtctaggaag atggactgcc ctcatttttg cggccttcac
     7501 cttaaccttc ctttacattt ttaggggaaa tgtggccagt ctttatggta acgacccaaa
     7561 atttatcgat ttgacagtgc gttttttaac ttatagtctt ttcttccagt tagcagatac
     7621 ctttgcggcg ccgcttcagg gaattttgcg ggggtataag gatacagtta ttccttttta
     7681 ccttggtttg cttggttatt ggggcgtagc aatccctgtg gctacgctat ttgattccct
     7741 aacagatttt ggagcttatt cttactggat cggcttgatt attagtttga ttgtgagcgg
     7801 ggcgctctac cgttggcgtt taactgtgat tatgaagaga tttgaatctt tagcaaaatc
     7861 caaatgctaa aaagtttgtg aaaaaatatt cttgacaaga aaatcccttg ttttcacggg
     7921 gttttctttt ttattttgtg aaatttcttt tagaaaaact ttgacagtat ttgccaaaag
     7981 cggtaaaata gtaaggtaag aagaaagtta gaaaggcaag aagatgtcaa ctttagataa
     8041 aaatcttttg ctagaaatgt tccgtaagat ggaagaaatc cgtcgcatgg acttaaaaat
     8101 tgcacaatta gtaaagaaag ggaaagtgcc aggaatgacg cacttttctg ttggtgaaga
     8161 ggcagctaac gtgggggcta tgttagctct caatccagat gatctgatta cctcaaacca
     8221 ccgtggacac gggcaagcta ttgctaaagg gattgacctc aacggaatga tggctgaaat
     8281 ccttggtaaa tacactggaa cctgtaaagg gaaaggtgga tctatgcata tcgctgacct
     8341 tgatgctggg aaccttggtg ccaatggtat cgtaggtggt ggtatgggga tcgctgtcgg
     8401 tgcagccctc agtcagcaaa tgcaaaatac cggtaaaatc gttgtctgct tctttggaga
     8461 tggtgcgacc aatgaaggtg ttttccacga agcagtgaac atggcttcta tctggaacct
     8521 gccagtcatt ttctattgca ttaacaacgg ttacggtatc tctgcggata tcaagaaaat
     8581 gaccaatata gaacatatcc atcaacgtag cgccgcttat ggaattcctg gaatgttcat
     8641 cgaagacggt aacaatgtca tcgatgtcta tgaaggattt cagaaagctg tggaccatgt
     8701 tcgcagtggc aatggtccag tcttgattga aagtgtaact tatcgctggc tcggtcactc
     8761 atcatctgac cctggtaaat atcgtacgcg tgaagaagtg gaattgtgga aacaaaaaga
     8821 tccaatcgaa aacctccgca attaccttat tgaaaataac attgcaagtg ccgaagaatt
     8881 ggaagaaatc caagcgcaag taaaggaagc agtagaagct tctgttaaat ttgcagagga
     8941 aagcccattc ccaccgcttg aatcagcctt tgaagatatt tacgcagact aaggagaaag
     9001 agataaaatg gaaacaaaaa caatgtcctt ccgtgacacc attatccttg ctatgtctga
     9061 ggaaatgcgt cgcgatgaaa atgtgttctt gatgggagaa gacgtcggtg tcttcggagg
     9121 agacttcggt acttctgttg gaatgcttga agaatttggt ccagaacgtg tccgtgactg
     9181 tccgatttct gaagctgcca tctctggagc agcagcagga gcagccatga caggacttcg
     9241 tccaatcgtc gatatgacct tcatggactt ctctgttatt gccatggaca atatcgtcaa
     9301 ccaagctgct aaaacacgtt acatgtttgg tggtaaaggt caggttccaa tgactgttcg
     9361 ttgtgcagct ggtaacggag ttggctctgc cgcccagcac tcacaatctc tagagtcttg
     9421 gtttactcac attccaggac ttaaggttgt ggcacctgga acacctgcgg acatgaaagg
     9481 cctgcttaag tcttctatcc gtgataacaa cccagttatt atacttgagt acaagtcaga
     9541 atttaaccaa aaaggggaag tgccagttga tccagactac acaattccac ttggggttgg
     9601 tgaaatcaaa cgccaaggaa cggatgtaac agttgttact tatggaaaaa tgcttcgccg
     9661 tgtggttcaa gctgctgaag aattagcaga agaaggaatt tcagttgaaa ttgttgaccc
     9721 acgtaccctt gttccgcttg ataaggatat catcataaac tcagtgaaga agactggtaa
     9781 ggttgttctg gtcaacgatg cccacaaaac aagtggctat atcggtgaaa tttcagctat
     9841 tatttcagaa tcagaagcat ttgactatct agatgcacca attcgccgtt gtgcaggaga
     9901 agatgtgccg atgccttatg cacaaaacct agaaaatgca atgattccaa cagttgaaag
     9961 tatcaaagat gcaatccgaa aaacttataa caaagaatag ggttacataa gataagttat
    10021 gtaaataaaa tgaagctgac gagaaacttt gtatctttta ggtgcagagc tcttcgtcat
    10081 taatatctta gattagagca cgggctaaaa gctcggaaaa aagataaatc tccttggctt
    10141 catcgaagcc atcgtcgatt tcctattttt cagtcgcttt tgacgccctt tgtatcataa
    10201 atagaattgg agaggtcatg gctgatgaca agctaagagc gactcctgca gctagaaagt
    10261 tagcggatga tttagggata aacctctacg acgtttctgg ctcaggtgca aacggtcgtg
    10321 tccacaaaga agacgtggaa acttataaaa acacaaacgt ggttcgcatt tcgccacttg
    10381 caaaacgaat tgccctcgaa cataacattg cttggcagga aatccaagga accggtcatc
    10441 gtggtaaaat catgaagaag gatgttttgg ccctgcttcc tgaaaatatc gaaaacgata
    10501 gcatcaaatc tcctgctcag attgaaaaag tggaagaagt ccctgataac gtaacaccat
    10561 atggtgaaat cgagcgtatt ccaatgacac caatgcgtaa ggttattgcc caacgcatgg
    10621 ttgaatctta cttgactgcg ccaaccttca ccctcaacta tgaagttgat atgactgaaa
    10681 tgttggctct tcgtaagaag gttcttgagc caatcatgga agcaactggg aagaagacta
    10741 ctgtaacaga ccttctttcg cttgcagttg ttaagactct tatgaaacac ccatacatca
    10801 acgcttcatt gacagaagat ggcaagacta ttatcactca caactatgtc aatcttgcca
    10861 tggcagttgg gatggataat ggattgatga cacctgttgt ttacaatgct gagaagatga
    10921 gtctttcaga actggttgta gcctttaagg atgttattgg ccgtaccttg gatggtaaat
    10981 tggctccaag tgagttacaa aattcaacat tcacaatcag taatttggga atgtttggtg
    11041 ttcagtcctt tggtccgatt attaaccaac ccaactcagc tatccttggt gtcagttcga
    11101 caatcgagaa gccagttgtc gtcaatggtg aaattgtgat tcgcccaatc atgagtttag
    11161 gattaaccat tgaccaccgt gtcgtagatg gtatggctgg tgctaagttt atgaaggact
    11221 tgaaagagtt gattgaaacg ccaatctcaa tgttaattta agagcaagag tattcatttt
    11281 taagatatag ataaaggaag gaagacatgg ccttagaagt aattatgcca aaagccggcg
    11341 tggatatgac agaaggacaa atcgtccaat ggaataaaaa agtcggagaa tttgtaaaag
    11401 aaggagaaat ccttttggaa atcatgactg ataaagtcag catggaattg gaagccgaag
    11461 aagatgggta cttgattgcc attctcaaag gagatggtga aactgtccct gtaacggaag
    11521 ttatcggtta ccttggtgaa gaaagggaaa acatcccaac agctggagca gcatcgccag
    11581 aagctagccc tgtacctgta gcaagtacct caaacgatga tggtaagagc gatgatgctt
    11641 ttgatatcgt tgtgattggt ggaggtcctg ctggttatgt tgcagccatt aaagctgccc
    11701 aactcggcgg taaggttgcc cttgttgaga aatctgaact tggtggaacc tgcttaaacc
    11761 gtggatgtat tccaaccaag acctaccttc ataacgctga aattattgaa aatatcggtc
    11821 atgctgcaaa tcgtggtatc gtgattgaaa atcctaactt cactgttgat atggaaaaac
    11881 ttttagaaac taaatctaaa gttgttaata ctcttgttgg tggtgttgca ggacttcttc
    11941 gtagctacgg agttactgtt cataaaggaa ttggtacaat cactaaagac aagaacgtct
    12001 tggtaaatgg ttctgaattg cttgaaacca agaaaatcat tcttgctggt ggttcaaaag
    12061 tcagcaagat caacgtccct ggtatggaat ctccacttgt catgactagt gatgacattc
    12121 ttgaaatgaa cgaagtgcca gaaagccttg ttatcatcgg tggtggagtt gtcggtatcg
    12181 aactcggtca ggccttcatg acatttggtt caaaagtgac tgttatcgaa atgatggacc
    12241 gtatagttcc agctatggat gtggaagttt ctaagaacct tcgcttgatc cttgagcgta
    12301 aaggaatgac catcttgact ggtactaaac tgcaagaaat cattgaggaa aatggtcaac
    12361 ttcgtatcaa ggttgaagga aaagacgata tcatcgcaag caaagctctt ctttcaattg
    12421 gtcgtatgcc agaccttgaa ggtattggag aggttgagtt tgaattggat cgtggttgta
    12481 tcaaggtcaa cgaatacatg gaaacttcag ttccaggcat ttatgcacca ggtgacatca
    12541 acggtactaa gatgttggct cacgcagctt tccgcatggg tgaagtttcc gctgaaaatg
    12601 cccttaaagg aaatcatgca gttgccaaat tgaatttgac tcctgcagcc atctacactc
    12661 tccctgaagt agcagcagta ggtttgacag aagaacaagc ccgtgagaaa tacgatgttg
    12721 ccatcggtaa gtttaacttt gctgctaacg gtcgtgctat tgcatctgac gcagctcaag
    12781 gtttcgtaaa agttatcgct gataagaaat acggagaaat ccttggtgta cacattattg
    12841 gtcctgcagc cgcagaatta ataaacgaag catcaagcat catcgaaatg gaaatcactg
    12901 ttgaggaaat gctgaagacc atccacggac acccaactta ctctgaagtg atgtacgaag
    12961 cgtttgcaga tgttctagga atggccatcc attcacctaa gaaaaaataa aacgaagata
    13021 gattagactg gaaggttatt tccagtctct atcatataaa gtaaagggat atcatgaaat
    13081 acattatcaa tcattcaaat gacactgctt ttaatattgc cttggaagaa tacgccttta
    13141 aacacctttt ggatgaggat caaatcttcc tactttggat taacaagcca tctatcattg
    13201 ttggtcgtca ccagaacact atcgaagaaa tcaaccgtga ttatgttcgc gaaaatggta
    13261 ttgaggtagt tcgccgtatt agtggtggtg gagctgttta ccacgattta aataacctca
    13321 actacacgat catctccaaa gaagatgaaa ataaggcatt tgacttcaag agcttctcaa
    13381 ctccggttat caataccctg gctcaacttg gggttaaagc tgagttcaca ggtcgtaatg
    13441 accttgagat tgatggtaag aagttctgtg gcaatgccca agcctatatc aacggccgta
    13501 tcatgcacca cggttgcttg ctctttgacg ttgatttgtc agtcctcgca aatgccctta
    13561 aggtttcaaa agataaattt gaatcaaaag gtgtgaaatc cgtccgtgcc cgtgtaacca
    13621 atattatcaa tgaattacca aaaaaaatca cagtcgaaaa attccgtgat ttactcttgg
    13681 aatacatgaa aaaagagtac ccagagatga ctgaatacgt cttttcagaa gaagaattgg
    13741 ccgaaatcaa tcgcatcaag gatactaagt ttggaacttg ggactggaac tatggtaaat
    13801 cacctgaatt taacgtccgt cgtggaacca aattcactag tggtaaggtt gaagtctttg
    13861 ctaacgttac tgaatcaaaa atccaagaca tcaagattta tggtgacttc tttggtattg
    13921 aagacgttgc tgcagtagaa gatgtccttc gtggggtgaa atacgaacgc gaagatgttc
    13981 ttaaggcgct aaaaactatt gatatcacac gctacttcgc tggtattagc cgtgaagaaa
    14041 tcgctgaagc ggtagttgga taaatcaaaa agaagttggc caactaacca acttcttttt
    14101 ttgatattta tttacataat ttaaaatacg taaaatcata aactatccag agcattcttt
    14161 tgttcatcac taacaatatg ggtatagagg tcagtgactt gtgtgctagc atgtcctagt
    14221 tggtgactga ctaaaacttg tgatttagtc gcatcataga gcctagttgc tagtgtatgg
    14281 cgcagtttat ggggtgttac acgtacttta aaatcctctg agtatttagc aaccattttc
    14341 tcaacgctag aagcatcgat acgattagga acacctctgt agagagttaa aaaaagggct
    14401 gtatctgttt tttccgtttt atagcgttga ttccgaatgg ccagataatt ctctaaataa
    14461 ggtttagcaa aagcagcgac attgactgag tcacgtttgc caccttttcg agtaacatca
    14521 ataaccatca tttttagatt gagatctctt agatctagat taacagcttc agataagcga
    14581 acaccagatg ccaagagaag ggcaataatg gctaaatctc gttctttatt tttgttgaat
    14641 gatgagagag ctcgatttga aagctgttgt gggtgctctt gatcgatata agttagaaaa
    14701 cctgctgttt catcacctag aaagagtttt tgcttgatat tttcagctct ggcagcaagg
    14761 gtttctttct ttttcttggt tgaaactttt ttcattacat tacgatagaa ataaggttcc
    14821 ccctgatcat tttcaacctc ctcggttaga tatttgtaaa gactagaaag tgctgataag
    14881 gttcgattga tagttgtctg tgaaacacct tgttttgttg tattagcatt cagcaaggga
    14941 cgttcacgta gataaaggat aaaggattcc atgtctttct tagacatatt ttccaaaact
    15001 gataaaggaa tatcggatat tttatcagcg tttgaaatac cagactccaa aacccagctg
    15061 aaaaatcggt catattcctt aaggtattcg tacagggttg taaaactgta gggcacagcc
    15121 agcttagatt ggtagtattc cagaacatac cagggcatga gttgttttag tttgtcgatt
    15181 cgttccagta aaatctcacg tttcatgtat tttctccata agtaagtata ctagtagtat
    15241 agcatagact tgtatatttt tcaagaaaat tatagttttt cagaaagatg ttataggagt
    15301 ttttcaaagt gataaaaaga cttagaccta caaacaaaac agaataagga aaataataaa
    15361 aatctcaccc actaaacttc tgttaatact acataactga agagctatat aaggagctat
    15421 gagatacaaa acacattgta taatagattg aaactagaat agtacatctg gacttctaaa
    15481 acattgctag aaattgattt gattgtcctg attgttttat cctatttcta tttcatttct
    15541 ctatataatt gctattgttt tctttttcat gattttaatc ttactgtaca gatttgatac
    15601 agtaaactcc tttctagctt tatactttat catttctaac ttaattatag tcttattttt
    15661 aataaagttc aattatttat atgtaaaatt cctcatcagt aaaaaataac cgatctcatt
    15721 tctgagaatc ggttttctaa ataaatcaaa ctgatcattt acataacata aattatgtaa
    15781 atgatcatac tacaacaaca aatctttgac ttttccaatt tcactttttg gaataaccag
    15841 gtgaatcata tcacccagat acattctggt tgagccgtta actgtttggc tcttgccatt
    15901 atggacttga gttgtgatga ggacgttgtg tggtaagttg agttcatgaa cttgtttccc
    15961 agcaatttta tcagaaactg gtatttcgat aagtgtaact tctccttcgc tagatacttc
    16021 ttctggaagc attttttcca gcatggcttc atagactggc gtacctttga gcaaatccat
    16081 gataatataa gaaacaagag tgacaagacc aagtggcata aggttgcgaa tatctcctac
    16141 catctcagtt acgaggatca tagcggttaa gggagctttt gatatggctc caaaatagcc
    16201 actcattcct agaatgacaa atatagggaa ttgctcttga ctgacaagtc caagattgac
    16261 acagataaca ccaactaagg caccaagcaa agaaccaaga gctaaaatgg ggaggaaaat
    16321 tcctcctggc agtccacttc catagctaat catactccaa ataaagcgga ttaaaaagta
    16381 agctaataaa acttggaaac taaaattttg ttcagttaaa gaaaggacaa gctgatttcc
    16441 gccaccaatg atttgaggta agaagattcc gactggtatg ataaggataa aagccaagat
    16501 gggataataa gccctatcca aatggatttt ttgaccaatc aagtcataaa ctcttccaac
    16561 gtttaataca gctttctcat agagaaaacc tgaaaatcca aggaaaattc ccatgacgag
    16621 atatatccaa tactggtcta gggtcatggg aggaatgtta tctggcatat ccaatactgg
    16681 tgtcaaaccg aacatgagta gagacacaaa gtttgctacg atgctggctg ctagagttga
    16741 gacccagaaa aagcgcgaaa aatggtgata gacttcttct acaacaaaga gaagtgctgc
    16801 aataggagca ttaaaggctg cggctaaacc tgctgcagct ccactggcaa tcaaggaacg
    16861 ttcctctact ggactggatt tgagccactt ggcaattcct ttaccaccaa ctgctccaag
    16921 ttgaatgctg ggtccctctc gacccagcat gagtccactg gcaatagcaa gaatacctag
    16981 cacatatttt ttccaaagaa tgccccacca gttgagggac atgaggcctt tcagttcggc
    17041 ttcgacttga ggaattcctg agcctttaat atctttttct gaccgtgtta gtttggcact
    17101 gagccaacag atgagtatat aaaacaaaac cagtacaaaa agattgcgca ctaagtaccc
    17161 ttgatcttga taaactcctt gtatcaggtg gaagcccttt tcaattaaga aacgaaagga
    17221 tccgacgata attccaacga tgaggccgac aatgattcct cgaccaactt gggatagtat
    17281 agtgctggat gcaaaggcga attctttctt agaacggact atttccgact gttcctccat
    17341 ggtatttcct ttcaattaac tttctttttt acctaaaacc agtgatttaa cgggttcaaa
    17401 gctggttcgg tgaattgggg taactcctag ttttgtgagg ccttccagat gtttagctgt
    17461 gccatatcct gcattagtag cgaaatcata gccagagaac tgctgatcgt attctttcat
    17521 caattcatca cgtgttacct tggctactat agatgctgct gcgatagaga gggagttggc
    17581 atctcctttg ataatggagg tttgtgaaat gggcaagtcc agtttcatgg catcaatcaa
    17641 aaggtgctct ggttgagggc tgagctggga gattgcttct tgcatggcta gtttggttgc
    17701 ttcatagatg ttgacttggt cgatgacctg attatctatg ataccaattc caatcgacaa
    17761 ggcttggtct tgaacggctt ggaaaatctc cagatgtttc tttttaggaa ttttcttgct
    17821 gtcgttgaga cctttaatct tacaattttt aggtaaaata acggccgcag cgactacagg
    17881 accagcaaga ggaccacgac caacctcatc aatacctgca attaaggtca atccttgctt
    17941 ataaagttct ttttcataag aaagcatgga ttccaagcgc aaattttcat ctaattcagc
    18001 ttgaatggct cttttacgct tgctgatttc cttttgaact cctgagcgat tatccttttc
    18061 aagctctaaa aaaatagggc tttctaactc cttgactgtc acaaggagtt ctttgatttc
    18121 tttaatcgtt gccatcgagg tcttccaatg tatctaaggt atagttaccg agtttgccat
    18181 cacggacttc cttcacgaag agactgtaaa aacggtcata gtcatcacgg aaaccgaggg
    18241 cgcgggtcat atccataata atcacaggcg cttcttcttc aattttcatt tgtttgaagc
    18301 gttcagccag cttttctgga taatgttctt tgaaataatt gataccaaaa atggtaacct
    18361 catccatagg aagcaactga tccttgatag ctccagtcaa tgccaactta agtgcaacag
    18421 tttcatcctc aaacttaggc cagagaatcc ccggtgtatc caagatttcc agatctttat
    18481 tggttttaag ccattgttga ccttttgtga cccctggctt gtttccaaca acagcaatct
    18541 ttttaccagc caaacggttc atcagagttg atttaccagc gtttggaatc ccaataatca
    18601 tggtacgcaa ggtttcaatc tgaatcccac gttctttctg gcgagcaatc ttatcagcca
    18661 tgagcttctt ggccgcatct gttacaactt ttacagtcac ttgctctttg gagttgatag
    18721 ctagcgtctg gattccttgt gattcaaaat actgacgcca ttccttggtc attgctggat
    18781 cagccaagtc ggccttgttt aaaatcaaga gttttggttt atcaccaaca atcttggtca
    18841 acataggatt ttgactagat agaggcaagc gtgcatctac taaaatcgtc acaaaatcaa
    18901 caaattttaa attttcctgc acctgtcgac gcgctttaga catgtgacca ggaaaccatt
    18961 gaatagtagc cattgagttt tttcctttcg tagcaaggat ttagatcccc tattttattt
    19021 tactatagtt tgaacataga gagttttcaa atgaatgatg tccaaacagt tcttgtttta
    19081 tgacatagaa atctacttta tattatatca tgccttcttt attttgtccg atgatagcct
    19141 tttaccctat cttttagcct atcttttacc ctatatagaa aaaagccatc aaatgatgac
    19201 ttttcagagg gagcaaaaac tgtcctcttt atattttaaa catttaaatg tcttctttaa
    19261 acagtttatt aaagaaaccg tctgtgtttt taagaagggc tttgaatact ttggatttca
    19321 agtctactgt atttttgaaa tgatctttaa ttttcggatc tgacatacca tcaatatctt
    19381 tttgaacagc ttcagcaaat aaagttttca actgagcata atttgtaatt tgacgaccat
    19441 caatttcaat agcagtaaat cctttttctg cttttgactt aacatctttg aagtattgtt
    19501 tcttccactc ttcaagatta ttgaatttat ccttagatac tttcttgata atgaaatcat
    19561 cacccaataa ttttttattg gttttgtcag cttcgccttg atatttgcta gatgcataac
    19621 taatgaatcc attttcataa ccaaaatagc cccacatacg gaaagtatta tgcttgaatg
    19681 atgcagctcc aggtgctcct tggctagtat taccaccata gataccggtc atcatattaa
    19741 tatttacgta agcactatca aaatcttctg gattatatgt tctgtcacta ggagcgccat
    19801 gttttgtcat caaattattg ttgactaaat catcaatgtt gttgataaca attttcttct
    19861 tctcatcttc agttaattca cgaactttat caaattgatg tagctctcca tttttgcgat
    19921 attgtctatc cattttcttg aaccatcgac taatattacc atgtaattta gggataacag
    19981 catcagcctc aagatggtct aacatcataa gagcatcatt ataatttctc atataacggt
    20041 cgatatcttc acgagttttc aatttatctg gatcataatt ataccattgg tcaccgtcat
    20101 tttcacgatg ataagccatg ttaagaccaa gagcaccata ttcaccgtta ccagatttat
    20161 caggtgtctg caacatacct tgcgcatagg cttcaacgtc tgtaccttga cggtgtctat
    20221 gcccaccgaa gtaaagcatt ctatcgttta cgtgtgttgt ttcgtgagta aaggctgaaa
    20281 taccaaacgg actaatcata tttgttccca tgaaataaac ttggtcatta ttattgggat
    20341 cttggaaaat agatgccata gctcccatgt accaatttgt agtatgataa cgccaagtag
    20401 gtccatataa ttcacgaata ggtgcaattg gtttattatt cttatcataa cccatacgat
    20461 ttacaccttc gacgttatga tagttttggt tatcccaaac aggtgttgga accctatttt
    20521 gactcttgag taattggtca cgtacgcttg gcaatgctaa acgcgaccag aagtcaagat
    20581 aagtttgttg tgcgtgagca acttctttaa ttttatcttt aaatgcttct cgttcagctt
    20641 ctgtatactt accatatttt tcaaaagagc tataagccat tgtattgtaa gtcgaaatca
    20701 agaacatatg tgcattctta agagttaata gaggtaggat catacgatta tgaacaccat
    20761 tattcaaacc atcaaatgca cgatgtcgtt tattaacaaa atctgggttc gttgttttag
    20821 gctccacaat gtatacgttc ttagccgcat ggataaacca atcatttaaa tcagtatcgt
    20881 tagtgaagag acgcatgttg taatctaaga agccatgtaa ttcagactta ccaatagttc
    20941 caccaatcat atcacgataa gcatccaaag ttctgtcacc cttgatattt cgctcacttg
    21001 atccaattcg aatcaagaag tcaagaaggt caactgactt accatagaaa tcaggtttaa
    21061 acatcatcaa gtttttgata gtcatatccc catacttgat atcatagtaa cgattaaggt
    21121 aagtaagagc catcacgatt ttagctttat tttcatccac cttcttaaca agtgctttca
    21181 tagctgcttc gtcactattt aattgatggt cagcattttc aatcaaagat ttgaccaatt
    21241 tatcaagatt agctttcgtt tcagaaaaac tttcttcgaa ataaaggtct cggatataac
    21301 cattcttacg ctcatccgaa ttagcattag gatctctaga tgtatctcgt ttatccatca
    21361 aagcacggac ttcgggagaa attaattcaa cagttgctaa cctatcttta atgtctttaa
    21421 tcaattgatc acgattttta tcaaccatgt ttggtgtata aacaatgtca tctaatccat
    21481 cgatactata ctcacgaact tgcgctactt tagactcttt ttgatgaaca gctttgattt
    21541 ctttagtctt atctgcatag tgaatcatca catgatcaat atctgataaa tcggtcacga
    21601 attgaccatc cttcataccc gtaacagaaa ggacttcttt cttagctaag tttgaatctt
    21661 ctgcaagttt gttaccttga ttgacaatcc attctttgtt ataaaatggt tgcagtttct
    21721 caatattacg ataagccaga tctcgatcta ctttatagtc ttgggttgac ttatattcat
    21781 cttctcttct ggttaaacga tttaagcgat tgaccacagg ttcattgatt tcgtatttat
    21841 ctgcggtaat tcctgtagct tcaattttag attttgcctc ttcctcagaa ataggtctga
    21901 tacgattttt atcttttgaa cgctggtagc tagaatgccc atcactaaca cctattacaa
    21961 cataatttcg tcttacatta tctcctgtcc agtagccatc atcataatca atatcactag
    22021 aaccatagaa aatttcacca ttaggtacct tcatcatgga aactgaatct tcgacagaac
    22081 cgtggtgctt catgcttcct acaacaccac cactttcaat cgcctcagtt acttgaattg
    22141 ttcccttagc aatagatttt ttgattgctc cttcctgacg aacagtattg ttatttcctt
    22201 cattttgtgt ccaataagca atgcccgcag ccttggcttt cttgccaata agatttgcgt
    22261 taacatacgc gcgttcgacg agtcctttcc agttctcacc aacgatacca gtcaagaatt
    22321 gtccaccatc gccgacagaa gttacgttac cgataaaggc cacatttctg agtgtacctt
    22381 ggttatcaat cttatcaata aatccagata cccaatcttt acctactata ttacctttaa
    22441 ccttaacatt ttcaatggta gttccacctt taataatatt cgctacagct gcaacttggt
    22501 tcgcccatgg catatttatg tcaacattac caagattaat attcttaatc gttccaccaa
    22561 taacattgcc aaataacgga cgtgacaagt tatgaatggt atagtgatta tcaccatcac
    22621 tcaagagctt acctttgaaa tctttcgtaa catatgattt accaaatgct gatacatttg
    22681 tggcattaag gtcagcaccc aatttaaact cgtcatttgg atgttcttgc atatctctca
    22741 caagttcatt gaaattgtaa tatacattat tgacttttgg aactggtttt tcaatgtaat
    22801 gaatgtattc atttctagtc ttagatggat cgacatgttg aattaaatct tgagcgcttg
    22861 ctgtaatttt ataaagttct ttttcttttt cagttactaa ctcaatctta tcaactgcta
    22921 aacgagttac tttattatca cgactagtga tttttaagta cattttcttc acatcttcat
    22981 cagaaggttt ttcgctcatg aaatcactag gtatttcagt accatcatca tttaccttca
    23041 ctaaattagt agatgaaata tttttaattt cgaccttttt caactccaaa cgcaaagttt
    23101 tttcatccag tttagacgtt tcagaacctt gaccacgatc ataaatcatt gttgttgcaa
    23161 tcttataatc tttaaacaaa tccaaatttt gtacagcgtc agttaatttt tctttagata
    23221 aatcaacctt tttaactact gtatcaccat ctttaatttc agctataata cttgatattt
    23281 cagcattatc tttattatca agctcataat ttaagttggc agaacgattc atagcatctt
    23341 ctgttacttt ggtcaaggtc aaaacgggaa ctgttttttc ttttgtacct tttacaagaa
    23401 ttttaggttt catttctttc aaaattgtta ccgtttcaac tggttcacta atttttttgc
    23461 catgtaactc ttcatagcta gtaacaattt gacgttcgcc atcctcacca acttctttaa
    23521 cagaatcatc gcctataaat ttgctaggat cagattcttc tatagtggtt ttaggaatag
    23581 tctctgtacg aatatcggta tgtaaagaag gcaactcttc tctttctgat gcaacttctg
    23641 aaactgtaga tattggttca gtatactctg gaatgtcgtt aacaggtggt tcaatcaagt
    23701 ttccattttc gtccactcct gttgtaccta ctgattcagt atactctgga atgtcgttaa
    23761 caggtggttc aatcaagttt ccattttcgt cca
//
LOCUS       DAVDWM010000033        22665 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVDWM010000033 DAVDWM010000000
VERSION     DAVDWM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.033"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(1..354)
                     /locus_tag="VUW49_001471"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="VUW49_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(346..802)
                     /locus_tag="VUW49_001472"
                     /pseudo
     CDS             complement(346..802)
                     /locus_tag="VUW49_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            complement(804..1841)
                     /locus_tag="VUW49_001473"
     CDS             complement(804..1841)
                     /locus_tag="VUW49_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET7981371.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(1939..3264)
                     /gene="brnQ"
                     /locus_tag="VUW49_001474"
     CDS             complement(1939..3264)
                     /gene="brnQ"
                     /locus_tag="VUW49_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET7981372.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(3435..3527)
                     /locus_tag="VUW49_001475"
     CDS             complement(3435..3527)
                     /locus_tag="VUW49_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7981373.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(3570..3722)
                     /locus_tag="VUW49_001476"
     CDS             complement(3570..3722)
                     /locus_tag="VUW49_001476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7981374.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(3882..4460)
                     /locus_tag="VUW49_001477"
     CDS             complement(3882..4460)
                     /locus_tag="VUW49_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7981375.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     DFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(4514..5914)
                     /gene="pepV"
                     /locus_tag="VUW49_001478"
     CDS             complement(4514..5914)
                     /gene="pepV"
                     /locus_tag="VUW49_001478"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET7981376.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKVDAEHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLYFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFDGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(5927..6532)
                     /locus_tag="VUW49_001479"
     CDS             complement(5927..6532)
                     /locus_tag="VUW49_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7981377.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            6703..7503
                     /locus_tag="VUW49_001480"
     CDS             6703..7503
                     /locus_tag="VUW49_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7981378.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(7683..8516)
                     /locus_tag="VUW49_001481"
     CDS             complement(7683..8516)
                     /locus_tag="VUW49_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7981379.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKELPITASVRGNWDDCVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSKGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKDFFDF
                     L"
     gene            complement(8506..10350)
                     /gene="uvrC"
                     /locus_tag="VUW49_001482"
     CDS             complement(8506..10350)
                     /gene="uvrC"
                     /locus_tag="VUW49_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7981380.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(10390..11091)
                     /locus_tag="VUW49_001483"
     CDS             complement(10390..11091)
                     /locus_tag="VUW49_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET7981381.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPHYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(11170..12402)
                     /gene="murN"
                     /locus_tag="VUW49_001484"
     CDS             complement(11170..12402)
                     /gene="murN"
                     /locus_tag="VUW49_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET7981382.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNSIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(12407..13627)
                     /gene="murM"
                     /locus_tag="VUW49_001485"
     CDS             complement(12407..13627)
                     /gene="murM"
                     /locus_tag="VUW49_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET7981383.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFIIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(13739..14518)
                     /locus_tag="VUW49_001486"
     CDS             complement(13739..14518)
                     /locus_tag="VUW49_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET7981384.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(14587..16248)
                     /locus_tag="VUW49_001487"
     CDS             complement(14587..16248)
                     /locus_tag="VUW49_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET7981385.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFENHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMSYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(16423..18645)
                     /gene="recJ"
                     /locus_tag="VUW49_001488"
     CDS             complement(16423..18645)
                     /gene="recJ"
                     /locus_tag="VUW49_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET7981386.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYRFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            18922..19680
                     /locus_tag="VUW49_001489"
     CDS             18922..19680
                     /locus_tag="VUW49_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7981387.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHNGMNMIIVTHEIGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            19720..20484
                     /locus_tag="VUW49_001490"
     CDS             19720..20484
                     /locus_tag="VUW49_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7981388.1"
                     /translation="MISLFGLAAAKPVQADTSVADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQCLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            20497..20886
                     /locus_tag="VUW49_001491"
     CDS             20497..20886
                     /locus_tag="VUW49_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131131.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7981389.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSLHPDCPHLLQL"
     gene            21091..22281
                     /locus_tag="VUW49_001492"
     CDS             21091..22281
                     /locus_tag="VUW49_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET7981390.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKHLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRTYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAIYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         6594 a         5246 c         4133 g         6692 t
ORIGIN      
        1 tacttccgtc gcatcaatca ttaccgtgtc ctcaaaacta agaggagttc ttgaaatcgt
       61 aacaccactt tgaacaagag ttacttcaac ccattggctc cgacggatta agttgctttc
      121 gtggatacca aaatcagccg caatttcttc ataagttcaa tattctcgca catattgaag
      181 agtggccata agaaggtctt ctaggcttaa tttaggtttt cgtccacctt ttgcgtgttt
      241 aagttgataa gctgttttta atacagctaa catctcttca aaagtggtac gctgaacacc
      301 aacaagacgc ttaaatcgtg catcggttag ttgtttactt gcttcatcat tcatagaact
      361 acttaccata ttttgtttca caggaagtct aatctcatcc ggttttgctt ggcgggtttc
      421 tgcggtcatt ttttcaaaag ggacccacca gactggccca cagcccttga gaccgtggcc
      481 attcatatca cctcttcgtc tagggaaaag gtgccagtgg agatgggcat cgccatttcc
      541 tagaagttca atattcattt tctcagcagt aaagcccttg gcaactgcct cttggactaa
      601 actcatttct tctagaaaat ggagtcttgt gtccttttct aaaatggtgc aattccgtga
      661 catgttcctt ggctagaaag agactatagc ttgcaaaata ctgatggtct ccaatcacaa
      721 gatagcctgt ttccaactct ttgacaaagt agggattttc tcccttcttg atgagctcaa
      781 ttctctggca aataaggcac atatcaatcc actaaattgc ttctaagata ggcatcgacc
      841 attcgttctg ttgcgaccac cgagtcaata tgagtgcgct cataagaatg actagactcg
      901 atacccgcac caagaagggc atgcttgacc tctgcccctg cagacatagc cgctgaagca
      961 tccgaaccat aaaatggata gatgtccagc ttaaaaggaa tatcttgctc tttagccaag
     1021 gcaaccaagt gttgacggaa gtcatagtga taaggacctg aagcatcctt gacacagata
     1081 gatactgtgt actcatctgt ctgctggtca tctcccatag ctcccatatc cacagccaga
     1141 tattctacta cctgagcagg aatgttagaa ttagcaccat gtcctacttc ttcaaaaact
     1201 gaaaaagcaa aatgagttgt tactggcaat tcaatcttct ctttcttata aatacgaagc
     1261 aggttgagca aaatcgctgc gctgacctta tcatccaaat gacgagactt gataaaacct
     1321 gtatcagtca caacagttcg tggatcgaag ctgataaaat caccgacctc aatcccaaga
     1381 gcacgagttt ctttttcatt gcttactttt acatccaaac gcacttccat attgtcctgc
     1441 gtgcgctctg cagttcctgc atccttgtag acatggcaag aagtttggtg gatgaggatg
     1501 gttcctgata ccttttgacc tgtgctagcc acatgaacgg tacagttttc accttcaatc
     1561 atgttccaag gaaaaccacc gatacggtcc aatttaagac gaccatctgg tttgacagca
     1621 cgaacaatag cacccagcgt atccacatga gcagtcacat agcgatgttg ctcatcattt
     1681 tgacctttga tggtcacatt gacaccaccc ttagctgtgc gaactggctg gtaaccaaaa
     1741 ccttctagag tcttgactaa ataattcgaa atttcacgag tgaagcccgt tggcgatgcg
     1801 atggctgtca gttctttgat atattctact gtttgattca tttcttcacc tctttcgcta
     1861 cctattataa cacatttatc atcggataaa aaaagaaaat cactcctgta gacttggcta
     1921 caaaagtgat ttaagtgatt attccatttc aaaaacatcg ctttcttgct tgtttggtag
     1981 aaccaatgag agcaaaattc caacaatggc tggcaccaac catgggagag atgccttggc
     2041 aaaaggaaga acgctaacaa gatttgcaag aaactcaacc ttaaacgagc ttcctagtac
     2101 gcttgcaatg gcaataactg taaccacagc aattgtcaac tgcatacctg gttttgaaag
     2161 ggcaacaaat ttgttgacaa tgacaatcat aacgatagca atcgtgattg ggtacaagat
     2221 aaccagtact ggaattgagt acttgataat cgcatcaaga cccaaattgg caatagcaaa
     2281 tccaatcaag gtaaaggctg tcgcataaac tttgtagctg atttgtggga agcgctcatt
     2341 aaagaactca gctgttgaca caattaaacc aacagtcgtt gtgaagcagg ttacggtaac
     2401 catagctgca aggaagagtt gagctgttga gccaaagatt tcttgagtgg cttgtgacaa
     2461 gatgtaaaca cctggtgttc cacccttcat cgcttcagct ggtactggga aatgatttcc
     2521 aagaaaacct aaaccgatgt aaagagcgct gaaggcaagg gcaacaacga taccaacaac
     2581 ccaaatagtt gaaatgtatt ctttcttact tgaaaatcca agttgtttca aggtttgaac
     2641 tgcgattacg ctaaaggcca ctgaggcaag ggcgtccaag gtattgtaac cttctaggaa
     2701 acctgtacca aaggcagaag cttgataagc agctgaagca gcttgaggac ttgttccacc
     2761 atatttgata gctcccagaa cgaccaagat aacaatcaaa attgcaaaga ctggcgttaa
     2821 aatacgtcca atgcggtcta agatttttga tggattaagc gaaatcaaat aggctgccgc
     2881 aaaatacaga accgtaaata caatcaagcc aagtccttta tttgcatccg acaaaagggg
     2941 gctaatccct acttcgtaag ctgttgtagc agtacgtggg gtagcaaaga atggaccgat
     3001 tgacaagtaa agaactgaga ggtaaagagt cgcaaaccaa ggcgctatct tcgttgaaat
     3061 ctcgtagata tatcctttag gatttagcgt tccaataata agggtcaaga cggcgatacc
     3121 aacgcctgaa aagacaaaac ctgcgatggc aggaagaaaa tgttctccag atagagcacc
     3181 tagagaaggc ggaaaaatca agttccccgc accaaaaaat attccaaaca ggagcaaacc
     3241 tgttagggta ccttttttag ccataaaaat ctccttcata tttataaaat actgacctag
     3301 tataccacga ttaggagtct gaaaaaagca tcacgaggca aatattaaac gttttcaatt
     3361 ttcttaaaaa taagaattat ctaaaaataa aactctccta cccagtccac accaagtagg
     3421 agaaatgcaa aattttaaag ttcttcaaag gctaacatgc caccctggac attgataaca
     3481 ttataacctt gttctaatag gaattggcaa gcacgcgccg atctcattcc agatttgcaa
     3541 ataatataat gcaagcgatc tttgtccaat taatcataac tatcagccaa ttgactaagc
     3601 ggtaggttgt gggcaccttc taaatgaaga gctgcaaact catccacttc tctcacatcc
     3661 actagagaaa gctggtcgtt ttggtaaagc tggtaaaatg cgtcaaaggc tatttctttc
     3721 attctttttc ttctaatact cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc
     3781 gtgcgtatgt taccgacttc gtcagtttta tctgcaactt caaagctgta ctttgagcag
     3841 cctacgacta gcttcctagt ttgctctttg attttcattg actataaaat ggttttaatt
     3901 ctttttttca aatctggcac tacttctgcc tcaaaccaag gatttttggc catccagatt
     3961 tgatttcgtg gtgatgggtg aactagcgga aaataggctg gcagatagtc tttatagtgt
     4021 ttcaccctct ccgttacctt cccactgatt ttctcctgta aatagtaggc ttgggcatat
     4081 tgcccaatca agagggttaa ctgaatatca ggcaattcct gtaagagctg cggatgccat
     4141 ttttctgcaa aatctgtacg aggcggaaga tcacccgact tgccatgtcc tggaaagtag
     4201 aaatccatag gcaaaacagc aaaataacct gaattgtaaa aggtatcttc atccacacct
     4261 agccagtccc gcaagcggtc accactttta tctttccagt aaaggcctgc ttcttgagtt
     4321 ttaagtcccg gagcctgacc gatgatattg atgcgagcag tttttggcgc tgcaaagaga
     4381 ggctcaatgc cacgctctgt atagctggca ttctgcgaat ccgccatgat agcctgtttg
     4441 attctttcga tttgagacat ctactttccc tccaaaaaga agtccaagca taagatctca
     4501 gacttctatc gttttatttg atcaattcgt aaatagcttc ggcataaatt gctgctgctc
     4561 ggaagagatc gtccaaggca ataaattcat tggcttggtg catggtatca attgagtctg
     4621 ggaacatagc accataggca actccgcgct ctaacaagcg accaaaggtt ccaccaccga
     4681 tgacttgttc atgaccttta aggcctgttt gtttttcata gacattcaac aaggtttgca
     4741 caagtggatc ttccattggc acatagtgag gggtatgacc atgttctgat agactgacag
     4801 aagcaactgg caagttttct aggattgact tgatttgttc tggacttgtt ccttttggat
     4861 agcggatgtt aagggcaatg gtattatcag cacttgtttc atcgaagcgg aagacgccgg
     4921 cattcataga aagagcaccc atcttttcat ccacatgagc aatcttgaga ttttcaccct
     4981 catgatcgtt caagagaatt ttacctgcga tgtcaaggta gtctttggct ggaccatcaa
     5041 agccaaactg gctgaggaag agggcaaggt aagttgcgcc attgacacct gaagcaggca
     5101 tagcaccgtg ggctgattta ccaatgatcg tcaccttgta tttgccagct tcttcttgga
     5161 gttctcctct aagtttgtgt tctgcaacaa aggcatctag tttagcttgc aagtcagcca
     5221 aatcacctga aacgactgct gttgctgatt ctggtaccat attttcacgt aaaccacctg
     5281 taaagctgtg aagacgggca gcacctgtat tttctcctgc aaagtagagg tattccgtga
     5341 tatttccttt ttcaccattg atgattggaa attcagcatc tggagagaaa ccaaagtctg
     5401 gcttcgcaag tcctacgtgc tcaaagtagt agtccatgtc tgcccagcct gattcttcgt
     5461 ctgttccaac gatgaagcga actttcttag aagttggaag acccaattct ttgatgattt
     5521 tcaaaccata gtaacaagct gttgtaggac ccttatcgtc cgaagccccg cgcgcataaa
     5581 ggcgaccatc tttgatagtt ggtgtgtaag gatctgtgtc ccaaccgcta ccagcaggca
     5641 ccacatccat atgggcaaag attccgagaa cttcttctcc atcaccaaac tcaaaatgtc
     5701 ctgcgtagtt atcgacattc ttagttgggt agccatcacg gtctgcgatt tcaaggaatt
     5761 tctccaaggc ttttactgga ccaggcccaa atggatgttc agcatcaacc ttgctgtcat
     5821 cacgttctga attgatttcc aaaaggctaa acaagtcagc caagaggtct tctttgcgtt
     5881 tttctacttc tgctgtaaaa tcaattgctg tcattttttc ttctttctat cttttctcga
     5941 tgatttcatc tactggcaag cggtagcttg gttccaattt ttcgtctgta taacccactg
     6001 tgatcaagag ttctgggcgg aaacggtctt caatttccaa aacctcatta acttttgatt
     6061 tgtcaaatcc aagaataatg ttagaaccaa ttccttggtc tgtcaatgca agaactaagt
     6121 tcatggcaac caaacctgca ttgagagcta ggtagtcgct gacttgttgc tcactgtaac
     6181 gggcaaactc agctggcaga tttttcataa aatattgaag ttgctcttca gaaaagttat
     6241 tagcaccacc aacacgggca atcttacgag cacgtttggc taagtccgta tctgtaaaca
     6301 aggcaatggt tacaggcgct gatgatacct gctcaaagtt tgaaccataa gccaactttg
     6361 ccagttcagc atttttctca cgaacaacta caaatttcca aggctggctg ttgtgagcgc
     6421 ttggcgccaa ggttgcaatt tcgatagccg tacgcacatc ttttggatca acaggcttat
     6481 cagtgaaatg cttggtcgca tgacgttttt tatttaattc aagaaatttc ataatcgatt
     6541 tccttttcta attctactgc ttccattcta ccataatttg aaagagcttg cagggatttt
     6601 tcatgattaa gtttttctat cacagacata aaagatatat attggtttat caggaacatt
     6661 tctgataaac tagcaagtac tttacatttg aatggagaca gtatgaaaaa atttagccta
     6721 ttactagcta tcctaccatt tttggttgcc tgtgggaatc aagctacacc caaagagact
     6781 agcgctcaaa agacaatcgt ccttgctaca gctggcgacg tgccaccatt tgactacgaa
     6841 gataaaggca atctgacagg cttcgatatc gaagttctaa aggcagtaga tgaaaaactc
     6901 agcgactacg agattcaatt tcaaagaacc gcctgggaaa gtatcttccc aggacttgat
     6961 tctggtcact atcaggctgc ggccaataac ttgagttaca caaaagagcg cgctgaaaaa
     7021 tacctctact cacttccgat ttccaacaat cccctcgttc tcgtcagcaa caagaaaaat
     7081 cctttgactt ctcttgacca gatcgctggt aaaacaacac aagaggatac cggaacttct
     7141 aacgctcaat tcatcaataa ctggaatcag aaacacactg ataatcccgc tacaattgat
     7201 ttttctggtg aggatattgg taaacgaatc ctagaccttg ctaacggaga gtttgatttc
     7261 ctagtttttg acaaggtatc cgttcaaaag attatcaagg accgtggttt agacctctca
     7321 gtcgttgatt taccttctgc agatagcccc agcaattata tcattttctc aagcgaccaa
     7381 aaagagttta aagagaaatt tgataaagcg ctcaaagaac tctatcaaga cggaaccctt
     7441 gaaaaactca gcaataccta tctaggtggt tcttacctcc cagatcaatc tcagttacaa
     7501 taacactctt cgaaaatctc ttcaaaccac gtcagcttta tctgcaacct caaagctgtg
     7561 ctttgagcaa cctacggcta gcttcctagt ttgctctttg attttcattg agtataagat
     7621 atactaaaag cgattggacc agccaatcgt ttttagtatg cattggttat tttaggaaac
     7681 tcttacaaaa aatcaaaaaa atccttcaca tcctctacat acccatgctt ttctattaag
     7741 ctggctaata attccagatt gtgcccctga tagttatcgt cacgacgcag ttcttcatac
     7801 atttcgataa agggaagccc cttggacttg gccaattcta actcagcttc gtaatcataa
     7861 gcaactttac gaaattggat attgagtaat tccccatctt caacttctat cacggcatac
     7921 tgggcacggt gattttttaa cgcctcccaa ttaaaatagg gcatgccaat cgaccctgga
     7981 ttgatgattt gttgcccttt actgccgtag cgaagcaact gcttgtggac atgaccatag
     8041 acggccacgt ccgtttccgc atctagcagt tggtcaaatt tctctgtatc attctcaact
     8101 agcaagtcac caccatagtt tttgtcaggt aaattatgag agatagaaaa gcgcaatccg
     8161 tcaatttctt tcttttccag caaaggcaag cttcgtagcc agacaatcgt tgcaggatcc
     8221 attcgctcca tcaaatactg tgtcatacgc aagagctgaa cttcctgtgg gtcttctaag
     8281 ccatattgcc catctaaagc ctcaaggaca caatcatccc aattgcctcg aacacttgcc
     8341 gtgataggaa gttcctttaa cagggcgact aagtcatttg cgcctggacc aggaagaaaa
     8401 atatctccca gaagccaata ttcactggcc ccttgatttt tagcatctgc aatcactgct
     8461 tctagcgccg tcgcattgcc atgaacatct gataaaattg cgattttatg gttcattgtg
     8521 gtttccttcc gtttggtaat ctactctttc ttctgctact tgaggcaagg cttctccctg
     8581 cgggttcaac tttctttgca cagcctctgc aacgactcta ggtaccccaa cttcgacaat
     8641 ctcatccaca ctggcttcct tgattttggt caaagacttg aaatgcttca taagattctg
     8701 cttgcgttta ggtcccagac cgtcaatccc atccaattga gatgagaaag aatttttgga
     8761 gcgcagttgg cggtggaaag tgatagcaaa gcggtgcacc tcatcttgga tgcgttggag
     8821 gaggaaaaat tcctgagaat tgcgagacaa gtccaccacc tcaagcggat ctccaaagag
     8881 caattcatgg gtttggtgct tatcattctt ttgcagccca gcaattggaa tatccaagcc
     8941 cagttcctct tggataacct gcttagcgat attgacttga ccttgccccc catcaatcac
     9001 aatcaaatct ggaggagtca aagcctcacg ctgtactcga ccatagcgtc tgcgaatgac
     9061 ctctctcatg ctggcatagt cgtctggtcc aacaaccgtt tttatcttgt acttacggta
     9121 atccttctta ctcggtttac cgttgacaaa gaccaccata gccgaaacag ggctagttcc
     9181 catgatatta gagttatcga aggactcgat acgtactggg gtcgggattt ggagcaaacg
     9241 ccctagattt tcaatagctc cttgagtctt ttcgacagat ttttctagca gattgaactt
     9301 ctgctctaga ctaacacgag cattttttat ggctagattg accagttgtt ttttctctcc
     9361 acgttgaggc ttaagaatct tggaatccac caaggccttg acagcctctt cgtcaatatc
     9421 ctgcggaatc agtacctcat tgggaactag atgagatttt tcttgataga attgtcctac
     9481 ataggtcaaa aaatcctcat ctggatcatt gaagtagggg aagagattga catcgcgctc
     9541 gataagctta ccctgacgga caaagaaaac ctgcacacac atccagccct tatccacata
     9601 gtagccaaag acatcgcgat tttgcaaatc tttcgccatg acccgttgct tggttcgaag
     9661 cgttccaata gcctgaatca ggtcacggta ttccgccgca cgttcaaact ccatactttg
     9721 tgctgctact gccattttac tcttgagatc atcgatgatt ttgtcatcct gacctttcag
     9781 aaaatcagac acctcctgag ccatagactt gaaataagcc tcatccttct tacagatggt
     9841 gtgggccata cactggccga tatggtaata aaaacagacc ttagagggcg ggttggtaca
     9901 ttttcgaaaa gggaaaatcc tatctagaag tcgcttgatt tcattggctg cccccacatc
     9961 tggataaggg ccaaaataaa gaccaccgtc ctttttgacc tgacgagtaa tgataagccg
    10021 tggatagcgc tcattggtga ttttgataaa aggataagac ttatcatcct tgagcatgat
    10081 attgtacttg ggcttgtttt ccttgatcaa gttgatttcc agcagaagcg cctcaatatt
    10141 ggactcagtg acgataaatt caaaatccac aatttcagac accagagcct ctgtcttggt
    10201 atcatgactt ccacgaaaat aggaccgtac tcgattacgc agatttttag cctttcctac
    10261 atagataatg gtgccatttt tatccttatg aatgtagcaa ccagggctgg tcggcaagag
    10321 ctctagtttt gatttaatca agttgttcat agttccatta tagcaaaaaa agtagggaaa
    10381 gatagtaccc tactcactag tctcatatat ttttcggagg tttgcaacat cctcttcctg
    10441 gataccataa aaggaacctg ctggttgcat gacagacttc tcatctgtat ggtccaagcc
    10501 aatcgcatga cctaactcat gttctgccgt atggacaagg cgttcatagg agtagccata
    10561 gtgtggattg gacaaataat aatgattcaa ccgcaccgtt acggacaaaa attgccctgt
    10621 taagagatta gtttgacttt ccgcctctcc tgccacagga gtgcctccgt cgttcatctc
    10681 cgtagccgta atatccgcct tactagactc agtcacgagt tcaaagttaa aagcaccagt
    10741 ttgattccag ttctggattg cttctaaata agcctcttga aagcgtgaat ccatctgcgg
    10801 atccaggtaa atacgagcag aggcctgtgc ccaccttcct tcagaatgct ggtgactatc
    10861 tgtctggctt aacttaggca gttgccctgt tttctcccat tggtcaatac tttgttgacc
    10921 aatttttact gaccgttctg cttgctttag cgctccttga aaatccccgt tcagatacca
    10981 gagaagtccg aaagcaagca cacataagag cacaactatc caaaccagac gccaaaacaa
    11041 acgccacaca aaagaaaaga aagcccctat caaacgaaaa agccagcgca tgtctctcca
    11101 cctttctagc aaataagaat tattttacta aaagaagcta aaagaaatct aaatactgac
    11161 tttttcatct taacgtccta ctattttttt gagtaactgg atagctttgt attttaaagg
    11221 cgatggatgg taacggaaag tacccgcttt gcgtacaata tagccattaa aattctgttt
    11281 aaaacgcaaa acaccatcac ttccatcaaa aatcccttga atgcctagga aattgtattt
    11341 aggtattcca cgttttatgc tttccaacat aacatatttt tgaagcagtg caggggcata
    11401 gaacttatta aactcagtgt aggaaccact aaagagataa gtcgtttcct gaggcatata
    11461 aacaaataaa ctcccagcta aaacaatatc ttcttctcca tatttttcaa tcaagtctcg
    11521 cgcttctgct tttcgaactt caaacgtttc aaattgacta gaatattctc tcagttgatt
    11581 ttgttttttc tcagaatgag gatttttact caaatcaagt cgcaacttgt ccaagttttc
    11641 ttctagttta ctttgttcac cttgcaattt gctcatatac tccgaaaaat tcaagcttgc
    11701 tatgagaaac tccgcttgtt ctccaaaagt atcataaaaa tgctcataat attctaaact
    11761 tttatcacta tattctctac gttcagaggt ttcttttgtt atattcttaa aaatcgatag
    11821 ttcttcacgt tttaactttt tcaaccgaat gccaaaggtt tcagcctttt tcaccaaggg
    11881 tttacccttt ttgctaaaac ttttaagcaa actcttttca gttaattcag ttaaatcttt
    11941 atagtataac caatctggtt ctccacctgg gtaacctgtt gttaagccat caaattgata
    12001 acctaaatca gtcaaacctt gaataatact ttttttctca gcatctattg aattaccttg
    12061 gctatcaaaa gtttgataag tttcataagg ttttacaagc aactctaata caccattttg
    12121 cttggcatat tcttttaact ctgcataaaa aactggaaga gcatcttgtt gggtataaat
    12181 cggccccgaa ttgagttcca tatgcagacc acccagcatg ggcaagctat aaaccagagc
    12241 tgcaacttga atttctcctt cttgtttcaa agcaagataa acaattcgag cccctctttt
    12301 ttctagcaaa tcccccatct ggacagattg cataaaggaa cgagaagaaa cctgatcaga
    12361 ataagtctga aactcttctt tcgtgagtgt tgttagtgcc atatacttac tttctatgtt
    12421 tttttcttaa tgttttacgg aaatcaagag caagtcttaa cagaggatag agaggatgag
    12481 tgggcattgt aaattcaccc aagtattctt caatcgttgg attaaatttt tccttaaaat
    12541 gataaagtcc accattgaga gagttttcaa caccacctaa attttgccaa accatacctc
    12601 gctcaaaggc atagcgagcc gtttcatacc atgttaaaat tggtgcattg taacgtttaa
    12661 aatcatcatc cataccagca tatatattga cagaggtagt accaaattcc aaactcaaag
    12721 tagccgctaa aggaactctc gcttgaccta catctatata ttcctgcaag aaggtcaatt
    12781 cctctaacaa acgttctttt tccttcttct gcgcttctac ttttgaaatt cgagtcgact
    12841 cagtaaaggt ctcttccaag gctctatttt tcgctaactg ttcttctaat tcttgcgaac
    12901 gtttagaaac atccaaggtg gccaaggtga tataggcctt gtccttaaaa ttatctaaca
    12961 attttttata ataggcttca ttcctcaaat gaatctcttt tcgcttctca gtttttttca
    13021 tcaactccga aaatgaatct aatagttcca gtccaccata ttgaatctca agccctttgt
    13081 ttcgtgctgt tcgaatagcc tgttttgttg acttggaaag tttatcttct tcaaaatttt
    13141 ccttgtatat tttcgcctga atacgaggtt gaatggtgtc tcccatttcc tccgtttttc
    13201 ctgaccacct tactcccatt tgttgcaaac tatcaataat agccagattt tcaggaaatt
    13261 ctgtcttttc ctgattgatt aaactttgag ataggcaaat acttgggtca aaagtcacaa
    13321 aaaccgctct cttactgcga gcataggact taatagactg aatgataaaa ttcaagagtt
    13381 ctttatcccc ataatccaat ataggtcctc ttgggatgta aaacatttta tagcctagcg
    13441 gaagagttct aatcaaaata ctagctgtcg ccagtaattt ttcttccctg taaacaccaa
    13501 acttctcatg ttgccaatta gacttaactt cctcccaagc actactttgt aatacattgg
    13561 ctaattcatg ttctttgaca aactgatcat attctaatgt gggaatgcca atttgataac
    13621 ggtacatttc ctactctctt tcctccagta ttttattact attctactac tttcgtttga
    13681 aaagtctagt gaaaacatat tttattttag ttctactccc cctatgctga agttcaaact
    13741 aagtcagtct ctcttctaat ttgaaatcaa ttggtagggt tgttaaaaaa acttccaatt
    13801 gtttttccca gtagtaccac tcgtgagttc cagcactatg gctataggtc acatcaaaac
    13861 ctagtttttt gagatttttc actgcgagat tattggcttc gtacaagaaa tcctgttcgc
    13921 cacaccacgc ccaaagtttg gtctttttat ccgatttttt agccagactt tcaagagaat
    13981 agggactagt tgtccagtct ctaatctctc caaaaacacc tctccagtag gctggacttc
    14041 ccagattttg actttcagga gaaaagtttt gaaagctgag ggcacctgaa aaactagctg
    14101 catgagaaaa acgatttgtc gtaagagcca gtttgaagca gccgtagcct cccatagaaa
    14161 gaccagcgat aaaggtcttt tcacgcttgc tagtcatatt agggaagaag cgtttcagaa
    14221 cctgtggcaa ttcctctgct agagccgtgt agtagtcaaa accatactgg gtatcggtgt
    14281 accaaccatt gctggtattg ggcataacaa cgatgagatt agttcctcga agcaagcgtt
    14341 ctacattggt ccgcttaagc caactattat gattgccaga catcccgtgc aaaaggtaca
    14401 agacgggaat atcttcacac tctggttctt ccactcgatt ggcatcaggg tagaggacat
    14461 tcacccccca ctccatatcc aatacttgtg agtaatactc gattttcatt actgccatga
    14521 ttttctcctt ctatttttct ataagaaaaa gccgaaactc gactttctct ctgtttattt
    14581 caacgattat tttgcttcca tgactactgg taaaatagct ggacgacgct tggtttggtc
    14641 aaagagatac ttggtcaaat tatctcgaac cttcccttta agatctgccc agtcaaagtc
    14701 atctccttga agatactctt ctaccgtttg gttaatcaat tctgaacttt cacggagaat
    14761 atcgcgactc ttcttgagat aaacaaatcc acgcgtgtga acacgagccc tagccacaat
    14821 tttcttctca cgacggttga ctgtaatagc cacgatgaaa attccatcct ctgacaagac
    14881 cttacggtca cgaagaacaa catttccaac atcaccaatg gcattcccat caatcaagat
    14941 atctcctgct gaaaccgatc cagctggaac aaagtctcca ttctcgtaag acatggtcgt
    15001 ccccttttta ggaatgaaga tgtgttctgg caacatccca actgccatgg cagccttagc
    15061 gtgagcatcc aactcacgat actccccttg gacagggaag aggtacttag gttgcaaaag
    15121 attgatcatc agctgcaaat cacgcacatt tccgtgccct gatacatgta aactttgggt
    15181 aatcaatttg acaaccccac ctgcctgata aatcatattt tccacacgcg caacaaaggc
    15241 ttctttagca atagacggag ccgtagcaat atagaccagg tccccatcct tgatttctac
    15301 ataacgatgg cgaccaatcg acatcttacg aagtccattg ataggttcac ccatacgacc
    15361 tgtctcaaga ataatcaact catggttttc aaagcgagac atatctttag gcttaatcaa
    15421 gagaatttcg ttggctaaag acaacttctt aagacgaatc gctgtgcgga cgatattttc
    15481 aatatcaaat cctgtcaaga cgatacgtcg acctgtttta tccgcagcgt caaaaatctg
    15541 ctgaatacga gaaagattac tggaaacagc tgcaacgatg atacgacctt cccagtcagc
    15601 aatagtttgg gtaatttcat ccctaacttc actttcacta gccacctgaa tattgctgtc
    15661 tgcattggcc gaatcactga ggagagccag gacgccgtca cgaccaatct ctgccaaacg
    15721 agcaaaatca gttgcataag attcactagc cgtttggtca aatttgaagt cacctgtata
    15781 aacgatgctt ccttccgatg tcttcaagac aattcccaga ctctctggaa cggagtaagt
    15841 cgtagggaag aaggaaacca ctgtcccacc aaaatcaatc tccgtattct catcaatgac
    15901 atggaaatca ttaaatttct taacggcatc atttcctttg acaaagagct ttgccaactc
    15961 aatggtcaac tcagacccaa atacaggaac tttagcctct gccaagagat aaggtagagc
    16021 accaatggca tccgcatgcc cgtgggtcaa gaaaacccca gcaatacggt cgctattttc
    16081 aaaaaggtaa tccatgtttg gaatcaccac atcgacccct aattgttcat tttcaggata
    16141 ttttaaccct acattcaaaa caaaaatgga ctctccaatt tcagcaatgt acatattttt
    16201 tccattctca cgcacaccac caagtgttgt taaactgata ttactcattt ttcctccaaa
    16261 atcttagttt atcttgatta tagggcaact ctaccctctt attctaaaag ttctgatact
    16321 ttcagccgaa tcttttccta ccattatacc atttttttga tgattttcaa aatgaaggtc
    16381 tgttttcaaa aaagagttga tttcccaact ctttcctaac ttctactctt tttccatcaa
    16441 aaaatcataa atttcttgca ccgtacccag cgccatcatt tcttggtctt taacggtttg
    16501 tttgagattt tggtaaattt gactttctcc tatctcccgc tttggcgcct ctttattgac
    16561 tgtcatcaca ccatctttta tcgtcacaaa gcctagttct tcaaatactt gaatcatctt
    16621 gaccagcaag atttgttgaa tattaagata tgcagccaaa tctttcagct tgtagcgaat
    16681 atcaaactct gggaaccggt aaatagtctt gtacaatttg gcaaactgat ctctagtccc
    16741 ataacctgtc agataatagg ccttgtcaat atcatttttg aaatagacag cagagaaatg
    16801 ctgttcctga aaaatggtct tcagctgagt aatatcctct ggaatgtttt ttacgacaac
    16861 agcttcacta gccgcaagat ttggcagttc tccaggaaaa tccaagactg gaacaccttc
    16921 tggcaagact gcattttttc cacgaatgtt aaaaagttga acaccttcca ctcgcgcatc
    16981 caccatcatc aactggaggg cagtttggcc attccattgg ttgacagaca atttaaccgc
    17041 taactctaga ttcttggttt gagaaaactc tgtcgcccat ctgccttgac caaaggctac
    17101 cacttcaaaa ctcgcctcac ccttggaaat tttcagcttt agatgggcat tacctgcccc
    17161 catagtacga gcactttcga cctgaaaatt cttgatataa aaaataggtt tctgattatc
    17221 cattccaaaa ggagctaaac gttcaaaact tttgaccgtt tccaagctaa gtgcctccaa
    17281 atccaactct tcatctaggt ttaacttatt cttgccacca gcatctgcac ctttttcacg
    17341 aacataatct tccaaaacct gagataaatc tgagagttgc tcaacttcca gcgtcatacc
    17401 cgctgcacct gcatgacctc caaaggcgat gaagaggtct cgatggggat ccagagcttc
    17461 aaaaatatcg accgcttcca cactacgagc actgcccttg gcacgaccgt cttctatatt
    17521 aagaacaatg actgtctgtc ccaattcttc caataaacga ccagccacga ttcctagaac
    17581 cccaggattc cagccttcct tggccaagac ctgaaccttc ttctcaggat ccaccatggt
    17641 cttggcttct tcatagatag actgaacgat ttccttgcgc tcttcgtttt tctggtgaat
    17701 cataagggca atctcatgcg cttcctcatc atcaaatcca gtcaacaaat caatggcagg
    17761 attgggatca tccaagcggc ccaaggcatt caaacgagga gcaatctgga aaccaaccgt
    17821 ttcttctgtt acttcgttgg cagcaatccc agccatgtcc agcatttctt gcagaccaat
    17881 gcgctgggta tgacccaaca tttccagacc atattgaact aagatacgat tttcatccgt
    17941 cagactcacc atatctgcaa tagttccaat agcgaccaaa tcaagcaatt ccacttgcac
    18001 ttcttctaac agggcacaag ccaacttgaa agcaactcca caaccagcca aatatttaaa
    18061 aggataatcc gcatctggat gttcaggatg gacaatagca taagcatctg gcagggtttc
    18121 aggcatggaa tgatggtctg tcacaatgac atctactccc atagactgag ccaatgcaat
    18181 agcctcatga ccagcaaccc cattgtccac cgtcacaatc aaggaaattc cttcttgctc
    18241 gataaagtat ttataaacac tagcattagg gccatagcca tcggtaaaac gatttggcag
    18301 gtaaactcgg cactccgcac caagttgttc caaactttcc ttcacaatag aagccgaagt
    18361 catgccatcc gcatcatagt ctccataaac gagaatattt tccccttctt caatagcctg
    18421 acgaatccgc tccactgcct tgtccatatc atggagcaga taagcatcat gtaagtcctc
    18481 caaggaaggt tctaaaaact tcttcagact ttcttggtcc tgaatccctc tctcaaacaa
    18541 taaccgagcc acctcaggac ccagtccagc cttcttggct atctttgtaa aatccgcatc
    18601 ttctacctgc agggcaaact gccattcata agtaggtgtt atcaaaaaga catccactct
    18661 ttctaagaat tctattgctt cattataaca tgatttagac gttttctcta tccagattgc
    18721 ttttttataa cattttatag tatattgaaa ctagaatagt acacctctac ttctaaaata
    18781 ttgttagaaa tcgatttgac tgtcccgatc gatttgtcct gttcttattt cactatagtg
    18841 aattttttca gatatggttt gacaagaaca ttcttttatt gtagaatcgt gttacaaaat
    18901 ctaacataaa tgagatctct catggcttta gtagaattta aaaacgtcga aaaatattac
    18961 ggagactacc acgcactccg caacatcaat ctccgttttg aaaaaggaca agttgttgtc
    19021 ctgcttggac cttctggctc tgggaagtcc actcttatcc gtacgatcaa tggtttagag
    19081 gctgttgaca aaggaagtct cctagtcaat gggcaccaag ttgctggtgc cagccagaaa
    19141 gatttggtac ctcttcgcaa ggaagtcggc atggtttttc aacattttaa cctttatcca
    19201 cacaaaacgg tgttagaaaa cgtgacactt gcacccatta aagttctagg aattgataaa
    19261 aaagaagctg aaaaaaccgc ccaaaaatat ctggaatttg taaatatgtg ggacaagaaa
    19321 gattcctatc ccgccatgct atctggtgga caaaaacagc ggatcgccat cgctcgtggt
    19381 cttgctatgc atccggaact cctcctcttt gatgaaccaa catctgctct tgatcctgag
    19441 actatcggag atgttctagc agttatgcag aaactggcgc ataatgggat gaacatgatc
    19501 atcgttaccc acgaaatagg ctttgctcga gaggttgcgg accgcattat ctttatggcc
    19561 gacggagaag ttttagtaga tacgacagat gtcgataact tttttgacaa tccaagcaaa
    19621 cctcgtgccc aacaattcct cagcaaaatt atcaaccacg aaagtgacaa agtcaaataa
    19681 ggaggcgcct atgaaaaaaa attcttttta tcagcattat tgattagcct tttcggcctt
    19741 gctgctgcca aaccagtcca ggctgatact agtgtcgcag acattcaaaa aagaggcgaa
    19801 ctggttgtcg gtgtcaaaca agacgttccc aattttggtt acaaagatcc caagaccggt
    19861 acttattctg gtatcgaaac cgacttggcc aagatggtag ctgatgaact caaggtcaag
    19921 attcgctatg tgccggttac agcacaaacc cgcggccccc ttctagacaa tgaacaggtc
    19981 gatatggata tcgcgacctt taccatcacg gacgaacgca aaaaactcta caactttacc
    20041 agtccctact acacagacgc ttctggattt ttggtcaata aatctgccaa aatcaaaaag
    20101 attgaggacc taaacggcaa aaccatcgga gtcgcccaag gctccatcac ccaatgcctg
    20161 attactgaac tgggcaaaaa gaaaggtcta acctttaaat ttgtcgaact tggttcctac
    20221 ccagaattga ttacttccct tcacgctcat cgtatcgatg ccttttccgt tgaccgctct
    20281 attctgtctg gctacactag taaacgcaca gaactactag atgatagttt caagccatct
    20341 gactacggta ttgttaccaa gaaatcaaat acagagctca acgactatct tgataacttg
    20401 gttactaaat ggagcaagga tggtagtttg cagaaacttt atgaccgtta caagctcaaa
    20461 ccatctagcc atactgcaga ttaaggagga caccccatga cagatttatc atcttggaca
    20521 gcctattttc aggattttgg acaatttttc aatggtttcc tctttactct tgccctagcg
    20581 attggatcct ttatcctcgc catggtctta ggaatcttct ttggtgccat gtcaaccagt
    20641 aaacgtccaa ttttacgcat tttagctcac atctttgtgg aattttacca aaacactccc
    20701 ctcttggtgc agtttgttat tgttttttat ggcctacctc ttatcagtga ccatactatc
    20761 atgattccaa tttattggac agctgttctt tgcgtgggac tctatcacgg cgcttatatc
    20821 gctgaggtta ttcgttcagg gattcagtct ttacatcctg attgccctca tctacttcag
    20881 ctttaaccta gcaatttcta acctgtctca tatgctagca aaacgttggc aacaagctgc
    20941 agaataacaa attagctctc tagttcattg gagagttttt tgatgatagt agatatttat
    21001 agacctcaat ttgacaccat acattttcta tgataaaatg taacaaattt atttacaaca
    21061 aatcgaaatc taaatgaaat ggaattttct atgaaatcag tcaaaggact actctttatc
    21121 atagctagtt ttatcttgac tcttttgact tggatgaaca cttctcccca attcatgatt
    21181 ccaggactag ctttaacaag cctatctctg acttttatcc tagccactcg tctcccacta
    21241 ctagaaagct ggtttcacgg tttggagaag gtctacaccg tccacaaatt cacagccttt
    21301 ctctccatca tcctactaat ctttcataac tttagtatgg gcggtttgtg gggctctcgc
    21361 ttagctgctc agtttggcaa tcttgccatc tatatctttg ccagcatcat ccttgtcgcc
    21421 tatttaggca aatacatcca atacgaagct tggcgatgga ttcaccgcct ggtttaccta
    21481 gcctatattt taggactctt tcacatctac atgataatgg gcaatcgtct ccttacattt
    21541 aatcttctaa gttttcttgt tggtagctat gcccttttag gcttactagc tggtttttat
    21601 atcatttttc tatatcaaaa gatttccttc ccctatctag ggaaaattac ccatctcaaa
    21661 cacttaaatc atgatactag agaaattcaa atccatctta gcagaccttt caactatcaa
    21721 tcaggacaat ttgcctttct aaagattttc caagaaggct ttgaaagtgc tccgcatccc
    21781 ttttctatct caggaggtca tggtcaaact ctttacttta ctgttaaaaa ttcaggcgac
    21841 cataccaaga atatctatga taatcttcaa gccggcagca aagtaaccct agacagaact
    21901 tacggacaca tgatcataga agaaggacga gaaaatcagg tttggattgc tggaggtatt
    21961 gggatcaccc ccttcatctc ttacatccgt gaacatccta ttttagataa acaggttcac
    22021 ttctactata gcttccgtgg agatgaaaat gcaatctacc tagatttact ccgtaactat
    22081 gctcagaaaa atcctaattt tgaactccat ctaatcgaca gtacgaaaga cggctatctt
    22141 aattttgaac aaaaagaagt gcccgaacat gcaaccgtct atatgtgtgg tcctatttct
    22201 atgatgaagg cacttgccaa acagattaag aaacaaaatc caaaaacgga gcttatttac
    22261 gaaggattca agttcaaata agtactcata ctctcctggg atttcccagg agagtttttt
    22321 atagccaaat gaaataagac cggaacgatg gaattttggc attcaaattg attaggaaaa
    22381 tcaaatgaat ttatagaaat cttttagcag ttatattgtc ctattctagt ttcaaaatgc
    22441 tatagaagca gcattgtgct agtctagatt cagtttacta tactaaaacg agtagcttga
    22501 aatcaaaaaa cccaccctca cacaggcagg ttttatctgt attattcagc tagattatgc
    22561 ggctctataa tatttgtagt gggtaacccc cctatggata ttatggagcc tattttgtgt
    22621 agaaaaaaag tcccatatga cctataatga aaagcgacaa aacaa
//
LOCUS       DAVDWM010000034        22402 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVDWM010000034 DAVDWM010000000
VERSION     DAVDWM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.034"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..977)
                     /gene="dusB"
                     /locus_tag="VUW49_001493"
     CDS             complement(<1..977)
                     /gene="dusB"
                     /locus_tag="VUW49_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7981391.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGTISQASTLAEIEALLQLEKA"
     gene            complement(964..1836)
                     /gene="hslO"
                     /locus_tag="VUW49_001494"
     CDS             complement(964..1836)
                     /gene="hslO"
                     /locus_tag="VUW49_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET7981392.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2001..3428
                     /locus_tag="VUW49_001495"
     CDS             2001..3428
                     /locus_tag="VUW49_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7981393.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSSLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKTERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3587..5095
                     /gene="glpK"
                     /locus_tag="VUW49_001496"
     CDS             3587..5095
                     /gene="glpK"
                     /locus_tag="VUW49_001496"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET7981394.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5137..6963
                     /gene="glpO"
                     /locus_tag="VUW49_001497"
     CDS             5137..6963
                     /gene="glpO"
                     /locus_tag="VUW49_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET7981395.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTSAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7033..7737
                     /locus_tag="VUW49_001498"
     CDS             7033..7737
                     /locus_tag="VUW49_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET7981396.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            7968..8099
                     /locus_tag="VUW49_001499"
     CDS             7968..8099
                     /locus_tag="VUW49_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET7981397.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            8120..9670
                     /gene="dltA"
                     /locus_tag="VUW49_001500"
     CDS             8120..9670
                     /gene="dltA"
                     /locus_tag="VUW49_001500"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET7981398.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKSDSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            9667..10911
                     /gene="dltB"
                     /locus_tag="VUW49_001501"
     CDS             9667..10911
                     /gene="dltB"
                     /locus_tag="VUW49_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET7981399.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAEKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            10925..11164
                     /gene="dltC"
                     /locus_tag="VUW49_001502"
     CDS             10925..11164
                     /gene="dltC"
                     /locus_tag="VUW49_001502"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET7981400.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            11157..12425
                     /gene="dltD"
                     /locus_tag="VUW49_001503"
     CDS             11157..12425
                     /gene="dltD"
                     /locus_tag="VUW49_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET7981401.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            12580..13020
                     /gene="adcR"
                     /locus_tag="VUW49_001504"
     CDS             12580..13020
                     /gene="adcR"
                     /locus_tag="VUW49_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET7981402.1"
                     /translation="MRQLAKDIDAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            13020..13724
                     /locus_tag="VUW49_001505"
     CDS             13020..13724
                     /locus_tag="VUW49_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET7981403.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            13717..14523
                     /locus_tag="VUW49_001506"
     CDS             13717..14523
                     /locus_tag="VUW49_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7981404.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            14533..16038
                     /gene="adcA"
                     /locus_tag="VUW49_001507"
     CDS             14533..16038
                     /gene="adcA"
                     /locus_tag="VUW49_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET7981405.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGKEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEGTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(16180..16305)
                     /gene="comC"
                     /locus_tag="VUW49_001508"
     CDS             complement(16180..16305)
                     /gene="comC"
                     /locus_tag="VUW49_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET7981406.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            complement(16470..16543)
                     /locus_tag="VUW49_001509"
     tRNA            complement(16470..16543)
                     /locus_tag="VUW49_001509"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16507..16509),aa:Arg,seq:tct)
     gene            complement(16587..17066)
                     /gene="rlmH"
                     /locus_tag="VUW49_001510"
     CDS             complement(16587..17066)
                     /gene="rlmH"
                     /locus_tag="VUW49_001510"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET7981407.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17249..18430
                     /locus_tag="VUW49_001511"
     CDS             17249..18430
                     /locus_tag="VUW49_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET7981408.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            18488..19246
                     /locus_tag="VUW49_001512"
     CDS             18488..19246
                     /locus_tag="VUW49_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7981409.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            19459..20820
                     /gene="dnaA"
                     /locus_tag="VUW49_001513"
     CDS             19459..20820
                     /gene="dnaA"
                     /locus_tag="VUW49_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7981410.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            20979..22115
                     /gene="dnaN"
                     /locus_tag="VUW49_001514"
     CDS             20979..22115
                     /gene="dnaN"
                     /locus_tag="VUW49_001514"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7981411.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            22180..22374
                     /locus_tag="VUW49_001515"
     CDS             22180..22374
                     /locus_tag="VUW49_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET7981412.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
BASE COUNT         6712 a         4274 c         4774 g         6642 t
ORIGIN      
        1 gccttctcca attgcaagag ggcttcaatc tctgccaggg tgctggcttg cgaaatggtt
       61 ccacggagtt tggcagcgcc agatgttcca cggaggtagt gaggagcgag gccgcggaat
      121 tcacgaactg cgacgttttc tcctttgagg ttaatcaatc gtttcaagtg ttcgtaggcg
      181 atcttcatct tgtcttcaaa ggtcaaatca ggtaggattt ctcctgtttc aaagtaatgg
      241 ttgatttggt tgaagaggta aggatttccc atggcagctc ggccaatcat gactgcgtca
      301 gcaccaactt cttcgatgcg ttgcttggct tcttggacag tacggatatc accgttggcg
      361 atgaatggaa tcttggttag agcttgggca accttgtaaa gggtctcaag gtctgcgtgg
      421 ccagtataca tttgttcacg ggtacggcca tgcatggcga gggcagaaac acctgcagcc
      481 tcagcagcga gggcattttc tactgccaga gatgggtccg cccagccggt acgcattttg
      541 acagtaagtg ggatatcaag gacagactgg accttgttga tgatagagta gatcttgtca
      601 ggatccttga gccacatagc tccagcttcg ttcttcacga ttttgttgac agggcagccc
      661 atgttgatat cgacgatatc ggtcttggtg ttttcttgga tgaattctgc tgcgcgtgct
      721 aggctgtctt catcgctacc aaaaagttgg atagagacag ggttttcgcc ctcatcgata
      781 tgaagcatgt gcagggtttt ttcgttgttg tattggattc ccttgtcaga gaccatttcc
      841 attacaacga gtccagctcc gagctctttt gcgatggtac gaaaggctga gttggtcacg
      901 ccagccatag gcgctaaaac ggtacgattg ggaatctcaa cattgccaat cataaaaggt
      961 gtattaagat ttgtcacgaa tgagttcctc caggtccttt tcatcaaagt tgtaagtagt
     1021 ttggcagaat tgacaagtga tttctgcgcc gtggtcttcc tctttcattt cctgtaaatc
     1081 tgagcttgga aggctggcaa gagcgttcat aaagcgttca tggctacagt cacattggaa
     1141 acggatttct tcttcagaaa gacgcttgta ggcttcgtcc ccgtagatag ccttgaggag
     1201 ggcttcgata tggtcgtcgc tttcgagaag agtagagata gctggcattt cttggatgcg
     1261 tttttcaaag cgagcaatct cttctttctt ggctcctggc aagacttgaa ctaggaaacc
     1321 acctgcaacc ttgaccttgt cttcctcgtc caaaaggaca ttgaggccga ccgctgaagg
     1381 cgtttgttgg ctttcagtaa ggtaaaaggc aaggtcttca ccgatttctc cagagatgag
     1441 gggagttata gaattgtaag gatttccagt accgtagtct gtgataacga ggaattgacc
     1501 atttccaaca aaaggtccga ctaggacttc accagtcgca gtctttttga tgtcaacacc
     1561 aggattttga acgtagcctt tgacgttccc cttggtatca gcgacggtga taatagcacc
     1621 tagagagcta gatcccaaca ccttaactgt aagtttggta tttccttttt cattggctgc
     1681 gagaatctgg ctggcgataa gagttcgacc aagcgctaca gttgagctag cttgggtttg
     1741 atgtttttct tgagcagtgc ggacggtttc cgtgctatca aggacaaaag cacgaaaggc
     1801 tccgctttct gatatagttt taataatttt atccatagct actattttag cataaaaatg
     1861 cccaaagggg gagccgtgtg tttactgatt ttcaggataa tggaccagga aatcagcatg
     1921 aaaataaaaa gagaaacaga ttatttcagc atttgtaaga tttatgctat gcttaaggta
     1981 gaaaatgaaa ggggtaacaa atgtatttag gagatttgat ggagaaagcc gagtgtggtc
     2041 aattttcaat cctttccttt ctattacaag agtctcagac gaccgtcaag gctgtaatgg
     2101 aagaaacagg attttcaaaa gcaaccctaa ccaaatatgt caccctgctc aatgacaagg
     2161 ctttggatag tagcttagaa ctgactattc actcagaaga tgaaaatctg cgtctgtcta
     2221 tcggtgcagc taccaagggg agagatattc ggagcttgtt tttggagagt gctgttaaat
     2281 accagatttt ggtttatctt ctctaccacc aacagttttt agcccatcag ctggctcaag
     2341 aattggtgat tagcgaggct acgcttggtc gtcacttggc tggtttaaat cagattttgt
     2401 cagaatttga tttatccatc caaaatggac gttggcgagg tccagagcat cagattcgct
     2461 atttctattt ctgtcttttc cgaaaggttt ggtcgagtca ggaatgggaa ggtcacatgc
     2521 agaaaacaga gagaaaacag gagattgcca atttagagga aatctgcggt gcaagtttgt
     2581 ctgcggggca gaaattggac ttggttctct gggctcacat cagtcaacaa cgtcttcggg
     2641 tcaatgcctg tcagtttcaa gtcatagaag agaaaatgcg agggtatttt gacaatatct
     2701 tctatcttcg tttgctgaga aaggttccgt ccttttttgc tgggcaacat attccactag
     2761 gagttgagga tggtgagatg atgatattct tctcttttct cctatctcat cgcattcttc
     2821 ctcttcatac tatggagtat attcttggtt ttggagggca gttggcagat ttactgacgc
     2881 aattgattca agaaatgaag aaggaggaac tattggggga ttatacagag gaccatgtca
     2941 cctatgaact cagtcagctt tgtgctcaag tctatctcta taagggctat attttacagg
     3001 atcgctacaa gtaccagtta gagaatcgtc atccatattt actgatggaa catgatttta
     3061 aagagacagc agaggagatt tttcatgctc tacctgcttt tcaacagggg acagatttag
     3121 ataagaagat tctctgggaa tggctccagt taatcgaata tatggctgaa aacggtggcc
     3181 agcatatgcg gattggtctg gatttgacat ctggttttct tgtcttttca aggatggcag
     3241 ccattttgaa acggtatttg gaatacaatc gttttatcac cattgaagct tatgacccta
     3301 gtcggcatta tgatttgctg gttaccaata acccgattca taagaaggaa cagacaccag
     3361 tctattattt aaaaaatgac ttggatatgg aggatttggc agggattcgt cagttattat
     3421 tcacttaaaa ggcttggtta attcaggtct tttttgtgaa attcacacaa tctcctcaca
     3481 tttttttaaa aattaaaaaa agttgataaa caagaaagcg ctttattttg tatactagta
     3541 agtgtaaaga ggaaacacct caagatcttt atcaggagga cagtatatgt cacaagaaaa
     3601 atacatcatg gccattgacc agggaactac aagttctcgt gccatcattt tcaacaaaaa
     3661 aggggaaaag gttagctcga gtcaaaaaga gtttacccag attttccctc aggcaggttg
     3721 ggttgagcac aatgccaatg aaatttggaa ctctgttcag tcagttattg cgggtgcttt
     3781 catcgaaagt ggtgtcaagc caaatcaaat cgaggcaatc gggattacca accaacgtga
     3841 aacaacggtt gtctgggata agaaaacagg acttcctatc tacaatgcta tcgtttggca
     3901 gtcacgccag acagcacctt tggctgagca actaaaaagc caaggttatg tggaaaaatt
     3961 ccatgaaaag actggtttga ttattgatgc ttacttctct gctaccaagg ttcgttggat
     4021 tttggatcat gtagaaggcg ctcaagagcg agcagaaaaa ggggaattgc tctttggtac
     4081 tatcgatact tggttggttt ggaaattgac tgacggtgcg gctcacgtga ctgactactc
     4141 aaatgcagct cgtaccatgc tttataacat taaagaactc aaatgggatg atgaaatttt
     4201 ggaaatcctt aacattccga aggctatact tccagaagtt cgttctaact ccgaaatcta
     4261 cggcaagaca gctccattcc atttctacgg tggagaggtg ccaatctcag gtatggctgg
     4321 ggaccaacaa gcagccctct ttggacagtt ggcttttgag ccaggtatgg ttaagaatac
     4381 ttatggaaca ggctctttca tcatcatgaa tactggggaa gagatgcagt tgtctgaaaa
     4441 caacctcttg acaaccattg gttacggaat caacggtaag gtttattatg ccttggaagg
     4501 ttctatcttc atcgcaggaa gtgctattca gtggcttcgt gacggtcttc gcatggttga
     4561 aaattcacca gaatctgaaa aatacgctcg tgattctcac aacaacgatg aagtttatgt
     4621 cgttccagcc tttacaggtc taggcgctcc atactggaac caaaatgctc gtggttccgt
     4681 ctttggtttg actcgtggaa caagcaaaga agactttatc aaggcgactt tgcaatctat
     4741 tgcttatcaa gtgcgtgaca tcatcgacac catgcaagtg gatactcaga ccgccattca
     4801 agtattgaag gtggatggtg gtgcagccat gaacaacttc ctcatgcagt tccaggcgga
     4861 tattttaggc attgatattg cacgtgctaa aaacttggaa acaacagctc taggagcggc
     4921 cttcctagca ggtttatcag tagggtactg gaaagacttg gacgagttga aactcttgaa
     4981 cgagacagga gaactctttg agccatctat gaatgaatct cgcaaggaac aactctacaa
     5041 gggctggaag aaggctgtga aagcaactca agtctttgcg gaagtagacg actaatactg
     5101 gcagaataaa gcgatttatt tagaaagtgt gtaaatatgg aattttcaaa gaaaacacgt
     5161 gaattgtcaa ttaaaaaaat gcaggaacgc accctggacc tcttgattat cggtggagga
     5221 atcacagggg ctggtgtagc cttgcaggcg gcagctagcg gtcttgagac tggtttgatt
     5281 gaaatgcaag attttgcaga aggaacatct agtcgttcaa caaaattggt tcacggagga
     5341 cttcgttacc ttaaacaatt cgacgtagaa gtggtctcag atacggtttc tgaacgtgca
     5401 gtggttcaac aaatcgctcc acacattcca aaatcagacc caatgctctt accagtttac
     5461 gatgaagatg gagcgacctt tagcctcttc cgtctcaaag tagccatgga cttgtacgac
     5521 cttttagcag gtgtgagcaa cacgtcagct gcgaacaagg ttttgagcaa ggatcaagtc
     5581 ttggaacgcc agccaaactt gaagaaggaa ggcttggtag gaggtggagt gtatcttgac
     5641 ttccgtaaca acgatgcgcg tctcgtgatt gaaaacatca aacgtgccaa ccaagacggt
     5701 gccctcattg ccaaccacgt gaaggcagaa ggcttcctct ttgacgaaag tggcaagatt
     5761 acaggtgttg tagctcgtga tctcttgaca gaccaagtat ttgaaatcaa ggcccgtctg
     5821 gttattaata caacaggtcc ttggagtgat aaagtacgta atttgtctaa taagggaacg
     5881 caattctcac aaatgcgccc aactaaggga gttcacttgg tagtggattc aagcaaaatc
     5941 aaggtttcac agccagttta cttcgacaca ggtttgggtg acggtcgtat ggtctttgtt
     6001 ctcccacgtg aaaacaagac ttactttggt acaactgata cagactacac aggtgatttg
     6061 gagcatccaa aagtaactca agaagatgta gattatctac ttggcattgt caacaaccgc
     6121 tttccagaat ccaacatcac cattgatgat atcgaaagca gctgggcagg tcttcgtcca
     6181 ttgattgcag ggaacagtgc ctctgactat aatggtggaa ataacggtac catcagtgat
     6241 gaaagctttg acaacttgat tgcgactgtt gaatcttatc tctccaaaga aaaaacacgt
     6301 gaagatgttg agtctgctgt cagcaagctt gaaagtagca catctgagaa atatttggat
     6361 ccatctgcag tttctcgtgg gtctagcttg gaccgtgatg acaatggtct tttgactctt
     6421 gctggcggta aaatcacaga ctaccgtaag atggctgaag gagctatgga gcgcgtggtt
     6481 gacatcctca aagcagaatt tgaccgtagc tttaaattga tcaattctaa aacttaccct
     6541 gtttcaggtg gagaattgaa cccagcaaat gtggattcag aaatcgaagc ctttgcgcaa
     6601 cttggagtat cacgtggttt ggatagcaag gaagctcact atctggcaaa tctttacggt
     6661 tcaaatgcac cgaaagtctt tgcacttgct cacagcttgg aacaagcgcc aggactcagc
     6721 ttggcagata ctttgtccct tcactatgca atgcgcaacg agttggctct tagcccagtt
     6781 gacttccttc ttcgtcgtac caaccatatg ctctttatgc gtgatagctt ggatagcatc
     6841 gttgagccag ttttggatga aatgggacga ttctatgact ggacagaaga agaaaaagca
     6901 acttaccgtg ctgatgtcga agcagctctc gctaacaacg atttagcaga attaaaaaat
     6961 taagaaaaaa taaaagaggt ggagggcagc attccttgtc gctcgtccct tctttttaat
     7021 ggagacagaa agatgatgaa tgaattattt ggagaatttc tagggacttt aatcctgatt
     7081 cttctaggaa atggtgttgt tgcaggtgtg gttcttccta aaaccaagag caatagctca
     7141 ggttggattg tgattactat gggttggggg attgcagttg cggttgcagt atttgtatct
     7201 ggcaagctca gtccagctca tttaaatcca gctgtgacaa ttggtgtagc tttaaaaggt
     7261 gacttgcctt gggcttccgt tttgccttat atcttagccc agttcgcagg ggctatgctg
     7321 ggtcagattt tggtttggtt gcaattcaaa cctcactatg aggcagaaga aaatgcaggc
     7381 aatatcctgg caaccttcag tactggacca gccatcaagg atactgtatc aaacttgatt
     7441 agcgaaatcc ttggaacctt tgttttggtg ttgacaatct ttgctttggg tctttacgat
     7501 tttcaggcag gtatcggaac ctttgcagtg ggtaccttga ttgtcggtat cggtctatcg
     7561 ctaggtggga caacaggtta tgccttgaat cctgcgcgtg accttggacc tcgtatcatg
     7621 cacagtatcc ttccaatttc aaacaaggga gacggagact ggtcttatgc ttggattcct
     7681 gttgtgggac ctgttatcgg tgcagccttg gccgtgcttg tattctcact tttttagttt
     7741 agaaaataac tattattgct agagcttgag atgaaaatct caggcttttt tcttttgaaa
     7801 tagatataga aaaaccgcat aatccttaaa ataagctgaa atctattgga aacatacggt
     7861 aaacaattga aaagatacta tataaagtgg tacaatggta gaaaaataag ctagtaagaa
     7921 tcactcttat tttagcgaaa aattacagaa atgaatggtt tttcttgatg aaacagagaa
     7981 aagaattgta cctctttctt ggtcggacag ccttgtattt tcttatcttt ctagggctgc
     8041 tttacttctt tagctatctt ggtcagggtc aaggaagctt tatctataat gaattttaac
     8101 ttgaaggaga atccctattg tgtcaaataa accaatagca gatatgattg aaaccattga
     8161 gcattttgct cagacacagc ctagctatcc tgtctataat gttttggggc aggaacacac
     8221 ttatggcgat ttaaagtctg attcggatag tttggctgca gtcattgacc aactaggctt
     8281 gcctgagaag tctcctgtgg ttgtttttgg tggccaagaa tatgaaatgt tggcaacctt
     8341 tgtagcgctg actaagtcag gtcatgccta cattccaatt gatagccatt cggccttgga
     8401 gcgagtttca gctattttag aagtagcaga gccaagcttg attattgcca tttcagcctt
     8461 tcccttggag caggtttcta caccaatgat aaatctagct caggttcaag aagcctttgc
     8521 ccaagggaac aactatgaaa tcacgcatcc agtcaaggga gatgataatt actacattat
     8581 ctttacttct ggtacgactg gtaagcctaa gggagtgcag atttcacatg ataatctcct
     8641 cagctttaca aactggatga ttacggataa ggaatttgcg acaccgagtc gtccgcaaat
     8701 gctggcacag ccaccttatt cttttgactt gtctgtcatg tattgggcac cgaccttggc
     8761 acttggtggt acgcttttca ctcttccttc agtcatcact caggacttta agcaactctt
     8821 tgcggctatc ttttcattgc caatcgctat ctggacatca acaccatcct ttgcagatat
     8881 ggccatgttg tctgaatact tcaacagtga gaaaatgcct ggaatcacgc atttctactt
     8941 tgatggtgaa gaattgacgg tcaaaacagc tcaaaaactg cgcgagcgtt tcccaaatgc
     9001 ccgtatcatc aatgcttacg gcccaacaga agcgacagta gctctgtcag cagttgccgt
     9061 gacagacgag atgttagcga ctctcaaacg cctaccaatc ggctatacca aggctgattc
     9121 tccaaccttt atcattgacg aggaaggaaa taaactgcca aatggcgaac agggagaaat
     9181 cattgtttct gggccagctg tttcaaaagg ttatatgaaa aatcctgaaa aaacagcaga
     9241 agccttcttt gagtttgaag gtctgccagc ctatcacaca ggcgatgtgg gaaccatgac
     9301 agatgagggc ttgcttctct acggcggacg catggacttc cagattaagt ttaacggtta
     9361 ccgcattgag ttagaagatg tctctcaaaa cctcaacaag tctcgcttta tcgaatctgc
     9421 tgtcgcagta ccgcgctata acaaggacca caaggtacaa aatctattgg cttatgtcat
     9481 cttaaaagac ggtgttcgtg agcagtttga gcgagatatc gatattacca aggccatcaa
     9541 ggaagacctg acagacatca tgatgtccta tatgatgcca tctaaattcc tttaccgaga
     9601 cagtttgcca ctaactccaa atggaaagat tgacatcaaa ggattgatta acgaggtgaa
     9661 taagagatga tggagttttt tcaacagctt cctcatttag agccatatgg caatcctcag
     9721 tattttgttt atgtgattgc tgcaaccttg cccatcttta taggtctctt tttcaagaaa
     9781 cgctttgcct ggtatgaagt gttggtaagt ctcttcttta ttgtcaccat gttggtgggt
     9841 ggaaagacca atcaactagc tgccttgggt atttaccttt gctgggaaat attgctcctg
     9901 cttttctaca agcattatcg aaaaagcaag gatggcaagt gggtcttcta cttagttagt
     9961 tttctgtccc tacttccgat tatctttgtc aaggtgcaac cagctatcaa tggaacgcag
    10021 tctttgcttg ggttcttggg aatttcttac cttacctttc gttcggttgg aattgtcatc
    10081 gagctgagag atggagtgat taaggatttt accctctggg aattcctccg tttccttctc
    10141 ttcatgccaa ctttctcgag tggtccaatc gatcgcttta agcgatttaa tgaaaattat
    10201 caggctattc ctgagcgaga tgagttgatg gatatgttgg atgaatctgt ccgctatatc
    10261 atgtggggct ttttgtataa gtttatccta gctcatgttt taggagagac cttactacct
    10321 cctctgaaga atttagcctt gcagtcaggt ggcttcttta atctctatgc cttggcagtt
    10381 atgtatactt ttggtctgga gctcttcttt gactttgcag gttattctat gtttgctttg
    10441 gccatctcaa acttgatggg aatccgtagc cctatcaact ttaacaagcc ctttttatca
    10501 agggatttaa aggagttttg gaatcgctgg catatgagtc tgtccttctg gttccgtgac
    10561 tttgtcttta tgcgaatggt gatggtgtta accagaaaga aagtctttaa aaatcgtaat
    10621 gtaacctcaa gcatggccta cattgtaaat atgctgatta tgggattttg gcatggtgtg
    10681 acctggtact atatcgccta tggactcttt catggactag gcttggtcat caatgatgcc
    10741 tgggttcgca agaaaaaaac gctcaataag gaacggaaaa aagcagagaa ggctgcccta
    10801 cctgagaatc gctggattca gttgcttggc atggttgtca ctttccatgt tgtcatgttg
    10861 tcattcttaa tcttttctgg attcttgaat aatctatggt ttaaaaaata aaaggaaata
    10921 aaatatggat atcaaatcag aagttattga aattattgat gagttgttta tggaagatgt
    10981 ttctgacatg atggatgaag atctttttga tgcaggtgtc ttggatagta tgggaacggt
    11041 tgagttgatt gtggagattg agaaccgttt tgacattcgt gtccctgtaa cagagtttgg
    11101 tcgtgacgac tggaatacag ctaataaaat catagctggt attgtggagc tacaaaatgc
    11161 ttaaacgctt atggatgatc ttcggaccgg tcttgatagc tggtttgttg gtttttctgc
    11221 tcattttctt ttatcctact gagatgcatc ataatctagg agctgaaaag cgttcagcag
    11281 tggctactac tatcgatagt tttaaggagc gaagtcaaaa agtcagagca ctatctgatc
    11341 caaatgtgcg ttttgttccc ttctttggct ctagtgaatg gcttcgtttt gacggtgctc
    11401 atcctgcggt attagctgag aaatacaatc gttcctaccg tccttatctt ttaggacagg
    11461 ggggagctgc atcgcttaac caatattttg gaatgcaaca gatgttacca cagctggaga
    11521 ataaacaagt tgtgtatgtt atctcacctc agtggttcag taaaaatggc tatgatccag
    11581 cagccttcca gcagtatttt aatggagacc agttgactag ttttctgaaa catcaatctg
    11641 gggatcaggc tagtcaatat gcagcgactc gcttactgca acagttccca aacgtagcta
    11701 tgaaggacct ggttcagaag ttggcaagta aaggagaatt gtcgacagca gacaatgaaa
    11761 tgattgaatt attggctcgt tttaatgaac gccaagcctc cttttttggt cagttttcgg
    11821 ttagaggcta tgttaactac gataagcatg tagctaagta tttaaaaatc ttgccagacc
    11881 agttttctta tcaagcaata gaagatgttg tcaaagcaga tgctgaaaaa aatacttcca
    11941 ataatgagat gggaatggaa aattatttct ataatgagca gatcaagaag gatttgaaga
    12001 aattaaagga ttctcagaaa agctttacct atctcaagtc gccagagtat aatgacttgc
    12061 agttggtttt aacacagttt tctaaatcta aggtaaaccc gatttttatc attccacctg
    12121 ttaataaaaa atggatggac tatgctggtc tacgagagga tatgtaccaa caaacggtgc
    12181 agaagattcg ctaccagtta gaaagtcaag gttttaccaa tatagcagat ttttctaagg
    12241 acggcgggga gcctttcttt atgaaggaca ccattcacct tggttggttg ggttggttgg
    12301 cttttgacaa ggcagttgat cctttcctat ccaatcccac accagctccg acttaccatc
    12361 tgaatgagcg ctttttcagc aaagattggg cgacttatga tggagatgtc aaagaatttc
    12421 aatagataat ataatataga agagttcaag aatgaaacta gttttcacgt tttttcttga
    12481 ctcttttttt gcttgtgata taattaactg gtaaacaaaa tgtcaaagaa tagtattttt
    12541 ctcttaaaaa agagaagtct aaaaggaaag gagtcagata tgagacagct agcaaaggat
    12601 atcgatgctt ttttgaatga ggtgattttg caggcggaaa atcagcatga aatcctaata
    12661 ggtcattgca ctagcgaggt ggccctgacc aatactcagg agcatatcct tatgctcttg
    12721 tcagaggaat ctttaacaaa ttcagaattg gcccgtcgtc tcaatgtcag tcaggcggca
    12781 gttaccaagg ccattaagtc tttggtcaag gaagggatgt tggaaacatc taaagattct
    12841 aaagatgcgc gtgtgatttt ttatcagttg actgacttgg ctcgtccaat cgctgaggag
    12901 caccaccatc accatgagca tacactttta acctatgaac aagtggcgac tcagtttact
    12961 ccaaatgaac aaaaagtgat tcagcggttt ttgactgctt tagtaggaga aatcaaataa
    13021 tgagatatat tacggtagag gatttgtcct tctattatga taaggagcct gttcttgaac
    13081 atatcaatta tagtgttgat agtggggaat ttgttacctt gactggggaa aatggagcgg
    13141 ctaagacgac gctcatcaag gctagtcttg gaattctgca accacgcatt ggaaaggtgg
    13201 ctatttcaaa gacaaatacg caaggtaaga aattgagaat agcctatctt cctcaacaaa
    13261 ttgccagttt taatgctggt tttccaagta cggtctatga atttgtcaag tcgggtcgct
    13321 atccgagaaa aggctggttc cgtcgtttga atgctcatga tgaggagcat atcaaggcta
    13381 gtctggactc agttggcatg tgggaacatc gagacaaacg cttggggtct ctatctgggg
    13441 gacaaaagca gcgagcggtg attgcgcgta tgtttgcttc tgaccctgat gtgtttatcc
    13501 tagacgagcc gacaacgggg atggatgcag gaagtaaaaa tgaattttac gaactcatgc
    13561 accacagtgc ccatcatcat ggcaaggctg ttttgatgat tacccatgac cctgaagaag
    13621 ttaaggatta tgcggatcgc aatattcatc tagtccgtaa ccaagactcg ccatggcgtt
    13681 gtttcaatgt tcatgagaat ggccaggagg tgggccatgc ttagtttatt atcttacgac
    13741 tttatacaac gcgccttttt ggcggttatt gctatgagtc ttttctcacc ggtattggga
    13801 accttcctca tcttgcgtcg tcagagtttg atgagtgata cccttagcca cgtctcactt
    13861 tcaggtgtag cctttggtct ggttttgggg atttctccaa ctgtttctac tattgccatt
    13921 gtcttgattg cggcggtctt tctggagtat ctccgtacgg tttacaagag ctttatggaa
    13981 atcgggacag ctatcctcat gtcaacaggt ctggctgttt ctctgattgt catgagcaag
    14041 ggtaaaagct cgagttcaat gagtttggac caatatctct ttggttcgat cgtgactatc
    14101 agtgaagaac aggtcatttc cctctttgtc attgcggcgg ttgttttgat tttgaccttt
    14161 ctctttcttc gtcctatgta tatcttaact tttgacgaag atacggcctt tgtggatggc
    14221 ttgccagttc gtaccatgtc cattcttttt aacatggtga caggggtggc tattgccctt
    14281 atgattcctg cagcaggagc tcttctggta tcgaccatta tggtcttgcc agctagtatt
    14341 gccctgcgtc tggggaaaaa ctttaaatcg gttatgctgc ttgccagtgc gattggcttt
    14401 ttgggaatgg tagcaggact ttatatttcc tactatgcag aaacacctgc aagtgcaagt
    14461 attaccatta tttttgtaac tgtctttata ctaatcagtt tagtaagacg ttttatcaaa
    14521 taggagacga acgtgaaaaa aattagctta ttactagcca gtctatgtgc cttgttttta
    14581 gtggcttgtt ccaatcaaaa acaggcagat ggtaaactca atatcgtgac aaccttttac
    14641 cctgtctatg aatttaccaa gcaagtcgca ggagatacgg ctaatgtaga actcctaatc
    14701 ggtgctggga cagaacctca tgaatacgaa ccatctgcca aggcagttgc caaaatccaa
    14761 gatgcagata ccttcgttta tgaaaatgaa aacatggaaa catgggtacc taaattgcta
    14821 gataccttgg ataagaaaaa agtgaaaacc atcaaggcga caggcgatat gttgctcttg
    14881 ccaggtggca aggaagaaga gggagaccat gaccatggag aagaaggtca tcaccatgag
    14941 tttgaccccc atgtttggtt atcaccagtt cgtgccatta aactagtaga gcacatccgc
    15001 gacagcttgt cagcagatta tcctgataaa aaagagacct ttgagaagaa tgcagctgcc
    15061 tatatcgaaa aattgcaagc cttggataag gcttacgcag aaggcttgtc tcaagcaaaa
    15121 caaaagagct ttgtgactca acacgcagcc tttaactatc ttgccttgga ctatggactc
    15181 aaacaagtcg caatctcagg actttctcca gatgcagagc catcagctgc tcgcttggca
    15241 gaattgacag agtatgtcaa gaaaaataaa atcgcctata tctattttga agaaaatgcc
    15301 tcacaagccc ttgctaacac actttcaaaa gaagcaggtg tcaaaactga tgtcctcaat
    15361 cctttagaaa gtctgacaga agaggacacc aaggctggag aaaactacat ttccgtgatg
    15421 gagaaaaacc tcaaggcttt gaaacaaaca acagaccaag aaggcccagc aatcgaacct
    15481 gaaaaggcag agggtaccaa gacagtccaa aatggttact tcgaggatgc agctgttaag
    15541 gaccgcacct tgagtgatta tgcaggtaac tggcaatcag tttatccttt ccttgaagac
    15601 ggcacgtttg accaagtctt tgactacaag gctaagttga ctggtaagat gacccaggct
    15661 gagtacaagg cttactatac aaaaggctat cagacagatg tgactaagat taacattact
    15721 gataatacta tggaatttgt tcaaggtgga caaagcaaga aatacactta caagtatgtc
    15781 ggtaagaaaa ttttgactta caagaaaggc aatcgtggcg tgcgtttcct ctttgaagcc
    15841 acagatgctg acgctggaca attcaagtat gttcagttta gtgaccacaa tatcgcccca
    15901 gttaaggcag aacatttcca tatctttttt ggaggtacaa gccaagaaac tctatttgaa
    15961 gaaatggata attggccaac ctactaccca gataacctat ctggccaaga aatcgcccaa
    16021 gaaatgttgg cgcattgatg agaaatcgac tagttcataa gagctagtcg gttttttgat
    16081 actagatggc taacttcatt atagaccttt caataattaa aaaatgaaca ataactgtcc
    16141 caaatccaag taaatccatt actctttctc cttatttcat tacttttttc gtaggaaaag
    16201 aaaatcaagg atgattcttg aaatcctcat ctccccacct ttaatctttt gtaagtcttt
    16261 ttccttcaaa gctacaaact gttccaattt aactgtgttt ttcataataa aatctcctaa
    16321 aatgtttttt cttgtaagct aacttacaaa aaccattata caaaatggaa tttcgtttta
    16381 gataaaattc tctcaactgt catttttttc tcccaaagtg tactttttta agaaaaaagc
    16441 cgggaaaatt cccagctttg ctattatatt gatcccagca ggattcgaac ctgcgaccgt
    16501 tcgcttagaa ggcgaatgct ctatccagct gagctatgag acctaataca attattctac
    16561 caaaaattca attaaaagtc aattttctat ttatggtagg ggaatccctg ctgaatcgta
    16621 aaagcgcgat agatttgttc aacaagaact agtctcatta actgatgggg taaggttagg
    16681 cgaccaaaac tgacagaaag attggctcta ttttttacag atgatgataa tcctaaactt
    16741 cccccaataa taaaagtaag agtagaaaat ccttttatag aagtttcttc taactgctta
    16801 ctaaattctt ctgagaagaa agttttccct tcaatggcta acacaataac gaaatcacgg
    16861 tcagcaattt ttgataaaat tctctgacct tctatttcta aaatcttttg attttctgat
    16921 tcactggcct tatctggtgt tttttcatct gataactcaa tcatttcaaa cttagcaaat
    16981 ctagaaattc gttttgaata ctctgcgata ccatctttta aatacttttc tttcagtttc
    17041 ccaactgtta caactttaat tttcatgact ctattctaac atattctcta ttttttcaca
    17101 tcttattcac aaaataaaaa atagatttca attaagaaaa tcacaatttc aaaagagtta
    17161 tccacagttt gtgtaaaact tttgtgttta agttataatt aagctagtca gtttatactt
    17221 tcagtaattc aaacatatgg aggcaaatat gaaacatcta aaaacatttt acaaaaaatg
    17281 gtttcaatta ttagtcgtta tcgtcattag cttttttagt ggagccttgg gtagtttttc
    17341 aataactcaa ctaactcaaa aaagtagtgt aaacaactct aacaacaata gtactattac
    17401 acaaactgcc tataagaacg aaaattcaac aacacaggct gttaacaaag taaaagatgc
    17461 tgttgtttct gttattactt attcggcaaa cagacaaaat agcgtatttg gcaatgatga
    17521 tactgacaca gattctcagc gaatctctag tgaaggatct ggagttattt ataaaaagaa
    17581 tgataaagaa gcttacatcg tcaccaacaa tcacgttata aatggcgcca gcaaagtaga
    17641 tattcgattg tcagatggga ctaaagtacc tggagaaatt gtcggagctg acactttctc
    17701 tgatattgct gtcgtcaaaa tctcttcaga aaaagtgaca acagtagctg agtttggtga
    17761 ttctagtaag ttaactgtag gagaaactgc tattgccatc ggtagcccgt taggttctga
    17821 atatgcaaat actgtcactc aaggtatcgt atccagtctc aatagaaatg tatccttaag
    17881 atcggaagat ggacaagcta tttctacaaa agccatccaa actgatactg ctattaaccc
    17941 aggtaactct ggcggcccac tgatcaatat tcaagggcag gttatcggaa ttacctcaag
    18001 taaaattgct acaaatggag gaacatctgt agaaggtctt ggtttcgcaa ttcctgcaaa
    18061 tgatgctatc aatattattg aacagttaga aaaaaacgga aaagtgacgc gtccagcttt
    18121 gggaatccag atggttaatt tatctaatgt gagtacaagc gacatcagaa gactcaatat
    18181 tccaagtaat gttacatctg gtgtagttgt tcgttcggta caaagtaata tgcctgccaa
    18241 tggtcacctt gaaaaatacg atgtaattac aaaagtagat gacaaagaga ttgcttcatc
    18301 aacagactta caaagtgctc tttacaacca ttctatcgga gacaccatta agataaccta
    18361 ctatcgtaac gggaaagaag aaactacctc tatcaaactt aacaagagtt caggtgattt
    18421 agaatcttaa ttgacatcta tgtaaagaaa gctttacata agagaaaaga tgtgttagtg
    18481 tagaatcatg gaaaaatttg aaatgatttc tatcacagat atacaaaaaa atccctatca
    18541 accccgaaaa gaatttgata gagaaaagct agatgaacta gcacagtcta tcaaagaaaa
    18601 tggggtcatt caaccgatta ttgttcgtca atctcctgtt attggttatg aaatccttgc
    18661 aggagagaga cgctatcggg cttcactttt agctggtcta cggtctatcc cagctgttgt
    18721 taaacagatt tcagaccaag agatgatggt ccagtccatt attgaaaatt tacagagaga
    18781 aaatttaaac ccaatagaag aagcacgcgc ctatgaatct ctcgtagaga aaggattcac
    18841 ccatgctgaa attgcagata agatgggcaa gtctcgtcca tatatcagca actccattcg
    18901 tttactttcc ttgccagaac agattctctc agaagtagaa aatggcaaac tatcacaagc
    18961 ccatgcgcgt tccctagttg ggttaaataa ggaacaacaa gactatttct ttcaacggat
    19021 tatagaagaa gatatttctg taaggaagtt agaagctctt ctgacagaga aaaaacaaaa
    19081 gaaacagcaa aaaactaatc atttcataca aaatgaagaa aaacagttaa gaaaactact
    19141 cggattagat gtagaaatta aactatctaa aaaagacagt ggaaaaatca ttatttcttt
    19201 ttcaaatcaa gaagaatata gtagaattat caacagcctg aaataaggct gttcttttat
    19261 ttttttatct cacaaggtta tccactatgt ttttcgataa aaagcttaat aaatcaataa
    19321 tttcttcttt tatccccaac ctgtggataa agtttagtaa cattgtggat tatttttcac
    19381 agcttgtgga aaattcttgc tatctatggt aaaatatctc tagtattaaa cttttaaata
    19441 gtaaaggagg agaaaggatt gaaagaaaaa caattttgga atcgtatatt agaatttgca
    19501 caagaaagac tgactcgatc catgtatgat ttctatgcta ttcaagctga actcatcaag
    19561 gtagaggaaa atgttgccac tatatttcta cctcgctctg aaatggaaat ggtctgggaa
    19621 aaacaactaa aagatattat tgtagtagct ggttttgaaa tttatgacgc tgaaataact
    19681 ccccactata ttttcaccaa acctcaagat acgactagct cacaagttga agaagctaca
    19741 aatttaactc tttatgacta tagtccaaag ttagtatcta ttccttattc agatacggga
    19801 ttaaaagaaa agtatacctt tgataacttt attcaagggg atggaaatgt ttgggctgta
    19861 tcagccgctt tagctgtctc tgaagatttg gctctgacct ataaccctct ttttatctat
    19921 ggaggaccag gccttggtaa gactcactta ttaaacgcta ttggaaatga aattctaaaa
    19981 aatattccta atgcgcgtgt taaatatatc cctgccgaaa gctttattaa tgactttctt
    20041 gatcacctaa gacttgggga aatggaaaag tttaaaaaga cctatcgtag tcttgatctt
    20101 ttgttaatcg atgatataca gtcactcagc ggaaaaaaag tcgcaactca ggaagaattt
    20161 ttcaatacct ttaacgccct tcatgacaag caaaaacaga ttgtcctaac gagtgatcgt
    20221 agtccaaaac atctagaagg gctcgaggag aggcttgtca cgcgttttag ttggggattg
    20281 acacaaacta tcacaccccc tgactttgaa acacgtattg ccattttaca aagtaagacg
    20341 gaacatttag gctacaattt ccaaagtgat actctagaat acctagctgg gcaatttgat
    20401 tcaaatgttc gagatcttga gggagccatc aacgacatca ctttaattgc cagagtaaaa
    20461 aaaatcaagg atatcactat tgatattgct gcagaagcca ttagagcccg caaacaagat
    20521 gttagccaaa tgctcgtcat cccaattgat aaaatccaaa ctgaagttgg taacttttat
    20581 ggtgttagta tcaaagaaat gaagggaagt agacgccttc aaaatattgt tttggcccgt
    20641 caagtagcca tgtatttatc tagagaacta acagataata gtcttccaaa aattgggaag
    20701 gaatttgggg gaaaagatca taccacagtc attcatgccc atgccaaaat aaaatctttg
    20761 attgatcaag acgataattt acgtttagaa attgaatcaa tcaaaaagaa aatcaaataa
    20821 tttgtggata acttttagtt ttttatcttt tttatccaca ttttttaaac aagctaaaaa
    20881 acttgatatg acttgtttaa aggctgtttt ccacagattt cacagactct attattacta
    20941 ttatctttct aatactaaaa ataaataaag gagaatccat gattcatttt tcaattaata
    21001 aaaatttatt tctacaagca ttaaatacta ctaagagagc tattagttct aaaaatgcca
    21061 ttcctatttt atcaacagta aaaattgacg tgaccaatga aggtattact ttaattggtt
    21121 caaatggtca aatttcaatt gaaaatttta tttctcaaaa aaatgaagat gctggtttgt
    21181 taattacttc tttaggttcg atccttcttg aagcttcttt ctttatcaat gtagtatcta
    21241 gtttacctga tgtaactctt gattttaaag aaattgaaca aaatcaaatt gttttaacca
    21301 gtggaaaatc agaaattacc ctaaaaggaa aagatagcga acaatatcca cgaatccaag
    21361 aaatttcagc aagcactcct ttaatacttg aaacaaaatt actcaagaaa attattaatg
    21421 aaacagcctt tgctgcaagt acacaagaga gtcgtccgat tttaacaggt gtccacttcg
    21481 tattgagtca acacaaagag ttaaaaacag ttgcaacaga ctctcatcgc ctaagccaga
    21541 aaaaattgac tcttgaaaaa aatagtgatg attttgatgt cgtaattcct agccgttctc
    21601 tacgcgaatt ttcagcggta tttacagatg atatcgaaac tgtagagatt ttctttgcca
    21661 ataaccaaat cctctttaga agcgaaaata ttagcttcta tactcgtctc ctagaaggaa
    21721 actatcctga tacagatcgc ttgattccaa cagactttaa cactactatt acttttaatg
    21781 tggtaaactt acgccagtca atggagcgtg cccgtctttt atcaagtgcg actcaaaatg
    21841 gtactgtgaa acttgaaatt aaggatgggg ttgttagcgc ccatgttcac tctccagaag
    21901 ttggtaaagt aaacgaagaa atcgatactg atcaggttac tggtgaagat ttgaccatta
    21961 gtttcaaccc aacttacttg attgattctc ttaaagcttt aaatagcgaa aaggtgacta
    22021 ttagctttat ctcagctgtt cgtccattta ctcttgtgcc agcagatact gacgaagact
    22081 tcatgcagct cattacacca gttcgtacaa attaagtgaa agaggttgag cctggctcgc
    22141 ctcttttatg atataatcga aaaagaaaag gagagtagta tgtatcaagt tggaaatttt
    22201 gttgagatga aaaaatcaca cgcttgtaca atcaagtcaa ctggtaaaaa ggctaatcgt
    22261 tgggaaatta cacgtgtagg agcagatatc aaaataaaat gcagtaattg tgagcatgtt
    22321 gtcatgatgg ggcgatatga ttttgagcga aaaatgaata aaattattga ctgagaaccc
    22381 ttagttagag ggttagcact tt
//
LOCUS       DAVDWM010000035        21599 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVDWM010000035 DAVDWM010000000
VERSION     DAVDWM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.035"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..174
                     /locus_tag="VUW49_001516"
     CDS             <1..174
                     /locus_tag="VUW49_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981413.1"
                     /translation="NALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALN
                     IKKERTKFVLSQA"
     gene            complement(567..2294)
                     /gene="ezrA"
                     /locus_tag="VUW49_001517"
     CDS             complement(567..2294)
                     /gene="ezrA"
                     /locus_tag="VUW49_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET7981414.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2376..4322)
                     /gene="gyrB"
                     /locus_tag="VUW49_001518"
     CDS             complement(2376..4322)
                     /gene="gyrB"
                     /locus_tag="VUW49_001518"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET7981415.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVYVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4337..4900)
                     /locus_tag="VUW49_001519"
     CDS             complement(4337..4900)
                     /locus_tag="VUW49_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7981416.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4985..5539)
                     /locus_tag="VUW49_001520"
     CDS             complement(4985..5539)
                     /locus_tag="VUW49_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET7981417.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5550..6773)
                     /locus_tag="VUW49_001521"
     CDS             complement(5550..6773)
                     /locus_tag="VUW49_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET7981418.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6810..9260)
                     /locus_tag="VUW49_001522"
     CDS             complement(6810..9260)
                     /locus_tag="VUW49_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET7981419.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVGHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9460..10389)
                     /locus_tag="VUW49_001523"
     CDS             complement(9460..10389)
                     /locus_tag="VUW49_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET7981420.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDLIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10529..10816
                     /locus_tag="VUW49_001524"
     CDS             10529..10816
                     /locus_tag="VUW49_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET7981421.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10850..12184)
                     /gene="ciaH"
                     /locus_tag="VUW49_001525"
     CDS             complement(10850..12184)
                     /gene="ciaH"
                     /locus_tag="VUW49_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET7981422.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12174..12848)
                     /gene="ciaR"
                     /locus_tag="VUW49_001526"
     CDS             complement(12174..12848)
                     /gene="ciaR"
                     /locus_tag="VUW49_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET7981423.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12957..15503)
                     /locus_tag="VUW49_001527"
     CDS             complement(12957..15503)
                     /locus_tag="VUW49_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7981424.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15626..16066)
                     /locus_tag="VUW49_001528"
     CDS             complement(15626..16066)
                     /locus_tag="VUW49_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET7981425.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCVVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(16041..16910)
                     /locus_tag="VUW49_001529"
     CDS             complement(16041..16910)
                     /locus_tag="VUW49_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET7981426.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAESHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16952..17680)
                     /locus_tag="VUW49_001530"
     CDS             complement(16952..17680)
                     /locus_tag="VUW49_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981427.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17688..18167)
                     /locus_tag="VUW49_001531"
     CDS             complement(17688..18167)
                     /locus_tag="VUW49_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7981428.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKARGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(18178..18876)
                     /locus_tag="VUW49_001532"
     CDS             complement(18178..18876)
                     /locus_tag="VUW49_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET7981429.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18869..19135)
                     /locus_tag="VUW49_001533"
     CDS             complement(18869..19135)
                     /locus_tag="VUW49_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET7981430.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(19203..20129)
                     /locus_tag="VUW49_001534"
     CDS             complement(19203..20129)
                     /locus_tag="VUW49_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET7981431.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20204..21019)
                     /locus_tag="VUW49_001535"
     CDS             complement(20204..21019)
                     /locus_tag="VUW49_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981432.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLVGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVVTILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(21023..21343)
                     /locus_tag="VUW49_001536"
     CDS             complement(21023..21343)
                     /locus_tag="VUW49_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7981433.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21433..>21599)
                     /locus_tag="VUW49_001537"
     CDS             complement(21433..>21599)
                     /locus_tag="VUW49_001537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981434.1"
                     /translation="TDEVSSKHVFEVVDETDEVSSKHVFEVVDETDEVSNHTYGRATM
                     TWFEEIFEEY"
BASE COUNT         6539 a         4744 c         3770 g         6546 t
ORIGIN      
        1 aacgcccttc aactacacta ttctaatgcc aaactggaag cgaccaataa tctcatcaaa
       61 cttatcaagc gcaatgcctt tggttttcga aactttgaaa acttcaaaaa acggattttt
      121 atcgctttga acatcaaaaa agaaaggacg aaatttgtcc tttctcaagc ttagctgact
      181 tcaacccact acagttgaca aagagcccag attaggaact atccgtgagt tctctagtct
      241 ggagattttt caatagactt cgttattgaa cggttaccaa gttactcaaa gtacagattt
      301 gaagctgcag ataagactga cgaaatcact ttaatactaa atgaaaatca aaaagcaaac
      361 taagagcctc aggctgtact tgagtacggt aaggcgacgc tgacgtgatt tgaagagatt
      421 ttcgaagagt ataaaacact gttttgaggt ggtggataaa tcggctgggc aaaaactcaa
      481 tctcaggaga aaatgaagta aatctcccca caactataga aaaagatttg attctgctcc
      541 tcacacaata aaatcttttt cttttattaa aaacgaatcg tttcacgtgt tttctcatat
      601 gaggtaacaa agcgattggt tacaccaggc tctgccactt ccaatgcttg agaaatcttg
      661 tcaaatgaag cgtgataatc aaattctttt tcaaaaatat ctaaagcttc gttaaatgct
      721 tcttgaatgc gttcatcaaa tgagcgatag cggttagaat attgcaagag ttgctctgtc
      781 aaagttgcat attgtacaat attataagtt tccgtttcta aagcttccat atcattcgtt
      841 gcaatttcaa gaactcgggt aacagattca atgttaatca ttttttgttc taactcaacc
      901 attaaatcct cggtattatt gcttgccgta aagaataact tcaagaaagt ttgtggaata
      961 cctggcagat tgcgtttttc catgtatcgc ttgatagtat ggagacgatt gacataaaca
     1021 ttggcctttt gacgtgcatt aatatcatct ttctcaattt gtgtcaggcg ctcactaact
     1081 gaaatttgct catcttcaat atcttttagc tgagtttgta aatcctcaag attttcttca
     1141 agaactgaat aagcttgggt tggttcttct tgatttgaag ttacctcaac aatagctgcc
     1201 tcaaaactct ctaattctgt ctgaatacga cgaacatggc ttgcagctgt ctcaggaagt
     1261 aaataggtct tgttcaaacg tgcaatatct tctcccaata aagtattatt ctctttcata
     1321 tgttgaagat aagttggaag agttgcaagt agattttcca ctactttctg agcagcaatt
     1381 tctcgagtaa aaatatcata caaggcattg atttcctctt gtgcctgtcc attctcatat
     1441 tcggcattat ccaattccaa ctgacgaata ttctcttggt ttttcttgaa tgcttcataa
     1501 agcaagtgga aacgcgcttc aatatccgtt tcaacaaaat gataattagc atcaattagt
     1561 ttacgataac cggcttccaa atcctgtaat tgatctggca attctgtaga aagcgtcgta
     1621 accaaggctg gaacacgatc cacaatatga cttaaggcca aaatgtgatt ttctgtatta
     1681 tccaaaatca ctgcggcttc cacagggtca cccgatgaat tcaaggttac aaattgtgaa
     1741 aattcagatt ggatattttc taattgtttt tcaatttcat ccaaggcttg accatactgt
     1801 tctgaatttt cagcaactct atgctgaagt tcctcaaata aatccaaagc atgaagaaca
     1861 cgaccactat ttttagattc ttgcttctct aagtctgcca aagcattgcg aattgccgca
     1921 atatcttctt caatcaaagt aatttgactc tcaatttggt caatttgatg actggccttg
     1981 agaaaacgaa atgaatggtt ataaccttct gcttcaaaga gattattttc aatatcggca
     2041 aaagagttga gagataaatc gacccatttt tgattccatt cacggaaagc cacttgactt
     2101 tgtccaatca agtgcatatt ttttacagct tctacttcat catttactgg aagattgtat
     2161 agttcttctt ttctttcttc tagtgcctct aatctcccct cgtttcgctt acgtagaaag
     2221 attgccacta cataagccag aactaaaatg actgcaattg caaccattaa ataaattagt
     2281 tgtccattag acatatcaaa ctcctttttt acttgaaaca atcgtaacga ttatatcata
     2341 ttttttagac caagggaact atttctccct attttttaga catcaagtgt actgtagaca
     2401 gcattttctt cgataaactc acgacgaggc tctactcgat cccccatcaa catatcaaag
     2461 attttatctg cttctgcagc atcatctaca gaaactctag ccatcaagcg atgttcggga
     2521 tccatggttg tttcccacag ctgatgatcg tccatttcac ctagcccctt ataacgctga
     2581 atagtcggtt tggtacgacc ttcactataa cgggctaaag cttcttggag tttgatttct
     2641 tgatctgcac ccggctggat atattcttta atctcgcttc caaccttgac accatagatt
     2701 ggtggttggg caatataaac ataaccagct tctaggattg gtttcatata acgataaatc
     2761 aaggttaaaa gaagggtacg aatatgggct ccatcgacat cggcatcggt catcaaaacg
     2821 agtttttggt aacgggcttt cgaaacatca aattctgcgc caaatcctgt tcccatggct
     2881 gtgaaaagac tacgaatttc ttcgttagct agaatcttat ccatacttgc tttttcaacg
     2941 ttcaaaatct taccgcgaat tggaaggata gcctgaaact cacggttacg accagatttg
     3001 gctgatccac cagctgagtc tccttcgacg atgaagagtt ctgtttcagc agggttatta
     3061 gaagaacagt ctgctagttt ccctggaagg ttggaaattt ccaaaccaga ttttttacgt
     3121 gtgacttcac gcgcacgctt ggcagccaca cgagccttgg cagctaaaat ccctttttcc
     3181 acgatacgtt tggcaatctg tggattttcc atgaggaaat cggagaaggc ttcactgaag
     3241 aggcgattgg taatcttgac cacttcgcta tttcccaatt tggtcttggt ttgtccttca
     3301 aactgtggat ttgggtgttt aactgagata actgcagtta atccttcgcg aacatcttcc
     3361 cctgttaaat tatcttcatt gtctttcagt aacttattct tacgagcata atcattgata
     3421 acacgagtca gtgccgtacg gaaaccttgt tcatgcgttc caccttcatg ggtatgaata
     3481 ttattggcga aactcatgac attttcatgg taacctgttg tgtactgcat ggctacttca
     3541 actgtaatat catccatctc accgtctgta tagattggtg tatcaaagat tacatccttg
     3601 ttctcgttga tatattcaac gtaactagca atcccacctt cataatgata atgcttggtt
     3661 tgttccaaac cttggcgctt atcagtgatg gaaatttgaa gaccgcgatt tagaaaggcc
     3721 aactcttgaa tccgtttatt taatttatca aaatcaaaga ttgttgtttc agtgaaaatt
     3781 tctgggtccg gtgtgaagtg aaccgttgtt ccagttctat ctgtatcccc aaccacctca
     3841 aggtctgcaa caacatgacc acgacggtat tcttggtaat gaatcttacc gtttttatgg
     3901 acataaacgt ctaattgagt ggaaagggca ttaactactg acgaccccac cccgtgaaga
     3961 ccacctgaaa ccttgtatcc accaccgccg aactttcctc cagcatgaag gactgtaaag
     4021 acggtctcaa cagcaggacg gcctgttttt tcctgaatat cgactgggat accacgccca
     4081 tcatccacaa cagtaatcga atcatctggc tcaataaaaa cttgaatatg gctggcaaat
     4141 cctgccaagg cctcgtcaat tgagttatca acaatttccc agactagatg gtgaagacct
     4201 tcttttgagg ttgatccaat gtacatccct ggacgcatac gaacagcctc taagccctct
     4261 aaaacttgaa tttgactggc atcataatcc tgtgcctgca gatttttgat ttcttctgtc
     4321 atcttattcc tttttcttat atgtctaata cttgtcttaa attcacgata ctggtaaaca
     4381 agtgggtatc aagtcctgca gcttgacctg cttcgatatc aatcggccga tcaccaatga
     4441 caagaccaga gctaatctga tacttttctc ttaaataaag catggattcg ggatttggct
     4501 ttctcttaaa gcctgagcta gaagtcacca cttctgtaaa ataagctgct atagaggttt
     4561 tttctaaaat ttccaaaacc tgatcatttc gatgagagac caaaaaatga cggccacctt
     4621 gatttgaaat gacttccaat aggtcagaaa ctccttcaaa taaaatcggg tgttcaagct
     4681 ctctggcttc attttccttg tacttttcta aaaaattctc taaattggga gcgaatgtct
     4741 caatcgcaaa aggagtagaa acctttaaag cttgatagac actgtcatgg tcttgtgtga
     4801 taccatacag tgccaatgtt tcaacaaatg cagctgttga agtttcataa ttatccagta
     4861 aagttccacc taaatcccag atgtaatcgt gatatttcat accctttatt atagcatttt
     4921 tttatgaaaa aagcgatgat ttccctgagt tttccacata aaaaacgaga gaataactcc
     4981 cgttttactg atttttacca aagacatctt gatagagcat tcctgttcgt aaactctctg
     5041 cgtcccctcc tagttgctcc accaactcgt aggcaaaggc aagggctgtt gaaggacccc
     5101 gactggttgt caactgacca tctaccacta ctgtttcctt gacgtagtga ccatcaagga
     5161 tttgctcttg aacgccgtca taacaagtgt atcgcttatt tttcaatatc tctgcttgat
     5221 taagggcaat tggtgccgca caaatggctg ctagtttctt cccttcttgc tcgaagcttt
     5281 gcaattcttg aatcaaggtc tgattatcac gtaaatgtgc agaaccaggc atacctccag
     5341 gaagaacaat catatcatag tctgataaat ctccatcaaa gacatgatct gctcttactt
     5401 ggattgcatg cgaacccgtt acttgctctt caaaaccaac catatcacat gtgatattgg
     5461 ctcgacgcaa gacatctaca actgtcaagg cttcaatttc ttcaaagccc tgagctaaca
     5521 taactgctac ttttaccatg attttctcct tatttaattt gttttaatac aacctttttc
     5581 cgtttgaatg ggacttttcc gctcttttct tcagctagat tagtctggta actcatcgat
     5641 aaaagcaaac caacaccaat cagattactg ataatagctg atcccccttg cgaaatgaaa
     5701 ggcaagggaa tccccgtcaa aggaagtagt ccagtcacag caccgatatt ctcaaagatg
     5761 tggaagagca acatcataat caaacctgtg gaaatataag tgtagaactg gttatttgat
     5821 ttaagagtaa tcttcaacat acggtaaatc aacatgagat agagggcaat aaccaggaca
     5881 gagccaataa agccaaaatc ttctgcaata accgtaaaaa tcatatctga ctctcgaact
     5941 gggataagca gattcgaagc attaaatccc tgaccaaata agccaccact cccaatggca
     6001 atctgccctt gagcctgctg gtaagtcgtt gtttgggcaa actcaaaggg attgagccaa
     6061 gccaaaatcc gattaatttg gtaggtcggc attccaatct ggtgaagaaa agctcgtccg
     6121 tccttgctaa taaagatagc taagaaacca gcaactcctg ttacagcagt cacaaatact
     6181 gggataataa ttttccaaga aacccctgat aataaaacga ttcctgagaa aatggctaca
     6241 aaaaccaaag ccgtccccaa gtcactttga agtgctaaaa gaactaggac tggaatggta
     6301 aagagaatca tccagaaaat taacaaaaag tccagcggaa ccgtgcgtct ccattcctta
     6361 tgtttctttg taaattggac aatgacacga gccaacatga ggatatagga tatcttcata
     6421 aattctgacg gttgaaatag ggtaattcca tttattgata cccagttttt ggcacccgtt
     6481 gatgcaacta agcttggatt ataaaataca atcggcaaga tcataagtcc caagcctaaa
     6541 atatatagaa agggggtcac cttccaaaga aattctgtat taaagagcat gaccacaaaa
     6601 ccaatcacaa gccccaaggc aatccaggcg acctgctgcc ctaaaatggg cagaatatta
     6661 ttgggataat catgactaac ggctatatag atagccacca caccgatgac cagtagaaaa
     6721 aatactggca agagcaaact ataatcgact ctagagtcga gagaacgttt catatgtact
     6781 aaccttatac tttcatacaa tactatttat caaagttcat taaaaaatct atcaatagcc
     6841 tcgtcaactt cggatcgaga gatggtttta acagtcgctt cttctgctag agatgctact
     6901 atttgtttgc cgtatcgttt tccgatgatt ctcctatcca aaataagagt taaggaacgt
     6961 tggtattcac gtctcatact tcttcccaaa gcctgtttta aacgaataat ggccattggc
     7021 aattgataat cataaaaggc atttttccct tcttgattca gttcttgatt aatctttttc
     7081 gtcaagggtt cttgaggatt ttggaaagga agcctcggta caacttgaat cacaaaagga
     7141 tggcttgaaa aatcaactcc ctcccagaaa cttgctgcac caagcaagat ttgttgttca
     7201 cctttttcaa agcgtttctt tagctgatga acatccccat ttttatactg ggccaagtgg
     7261 ccaactgtaa gtaaatccga tactgctaga agcatgtctt tagcggtaaa gagaaccaaa
     7321 atcggttgct ggaaagcttg aatttccact agtaaagcag ctacctctct ggcatagact
     7381 tctaaggagg tttctgttac cagagggaaa tctttgacca taaccacttc ttgttcctgt
     7441 tttccccgag attcaatctt gacaaatttg gcttcaggat agcctaaaag gtctgccaaa
     7501 gaaaccctct gactaatctc aagagtagct gatactccca agacttggca actctcagga
     7561 agtaaagagg acagaagagt acggcctgat tttgtagaag aaatctgaat tttcttttga
     7621 ctcgtttcag ttacttcaag ccagtaatca ccttcagctg taaaatagcg aaccagctca
     7681 tcaaagtctt ctacttccaa ttctgaaaaa tactgatgga gattgtccag agaatctaag
     7741 atatttttcc tagatttgcc agactgaaat tgttctatca agtagagaca ctcaaagcga
     7801 atactttcta gtatccgttg ttgaaccctg ttttcttctc ctactaaagc cttatcaatt
     7861 aaatcgataa tagattgtat atcgtaggtc tcttgaagca gattttctag agctaacaaa
     7921 atcttttgga cttcatcaat aatcagtaaa cggtcactga caaattcagg attatcttca
     7981 agtctggtta cgagataggc atgattagtc actaaaagct tgcaagtctc tgccctttct
     8041 tgactacgtt tccaaaaatc ttccgtcaca aataagctct gggatgataa attcccatca
     8101 tgacgaaggt ctgctagaaa atgttggtaa cggtagagtt gcccgatttc atccaaatct
     8161 cctgtctctg tctcagtaag ccagaccaag acttgcattt taaagcgtct aaataagcga
     8221 ttttcatcat tttcctgcaa ggaatgataa aaggcatcca acttcagata attttgtggt
     8281 ccctttaagc tatgaatatc tgtatggaac acttccttga ggcgtttacc ttcttcttcc
     8341 atgatttgat tttgaagaat ctttgtcgga acactaagaa caatttgtcg ctctttggat
     8401 tgagataaag cgggtaagag atagccatag gttttcccaa tccctgtcgg cgcttgaatc
     8461 agagagacag gttcatcttt caatagcaag ccaacctctt tagcaaaact ttcttgttcc
     8521 tccctcactt caaggttcaa cagagaaata tttttagaaa agtcttgaga tagttttcgt
     8581 ggctccagag aagcttccgt tttcttaaaa tatagacctt gaacttggac caagtctgga
     8641 gaactcagga tagattggtt gcgataagtt tcctcaataa ccaggtagga ctcatatagg
     8701 agagcgtcag ccatttccag caagcgttcc aagagacctt taggaagctg ggtcatcttt
     8761 ttccgtaaaa aaagaagtaa ttctgctgta gcttgggcat ctgaaagggc tgtgtgtgcg
     8821 tgtttaagag gaattcctaa ttctcgacac aaaatcggca aactatattt ttccagttca
     8881 gggaaaaaga cctgggccaa ttcgaccgta tcaacacgag ggtttcttag ctcatagcct
     8941 tcaaaaaata aattttccgc caagagatta gcatcaaact gaacattatg ggctacaaaa
     9001 atcccatcct ccaccaagtc aaatattttt ctggcaactt gcgaaaaatc aggtgcttgc
     9061 gccagacgtt ggtctgtcaa tcctgtcagt tctttgatat gagcatccaa gggttcatgt
     9121 ggattgacat ccgtcgtata gtgatcgacg atttctccgt cctcaatcac gacaattccc
     9181 acttggataa ttttagcctt actacctgtg ctagttgcct ctaaatccac cacaacatag
     9241 cgattaccaa tcgttttcat tataaaaaat tataccaaaa aaagggccat ttggcacctg
     9301 caaacatagg ataattttca aactcaagaa cactactttt cttaaaaatc ccatcgaata
     9361 gagcattcta gcctgctgat ttttgtaaaa tagtgaagaa atacgaaaaa ttgagagaag
     9421 aaatgtttgc atcttccttc tctcaacgaa tcatgtattt tatctaacca tgtcaggatc
     9481 gtagtcataa aatcctgtat caaggaaacg gtttttaggg tgaagtttac gaacttcttc
     9541 atccagcaag aaggcttgag catgactaaa gttgccctct tgaatcaagc tacgcgcttg
     9601 gataaagagt tttgcaatct caacaaagtt ctgaccagga gtgtagagcc cttctagttt
     9661 ccagcgagta aagccatgct ccaccaattc tgttaatttg atcatcaaat caaggtcatt
     9721 gttggcaaag atatgggtcc catgattatc ttcaaaaatg gaatagtggc tctctggatc
     9781 acttggctca gccaagaaga ggtcacgttt atgcgtcttt tcatcatcga tatgtgtaaa
     9841 gttatagtag ttttgcaaga gtggacgttt agaatgatgg atgacgctag caccgtaaac
     9901 caaaacttca gcaggaattt ccaaaatctc tggcattttg aaaagttcag ctgatggaat
     9961 ttcacgcgcc aaaacagcct cagatgcgcc agccttttgt ccccagaagt taatctgacg
    10021 actgctagtt accatggttg aagcatcgta gatggtctta aatgaataac catcgcggtt
    10081 aactacgtaa aagacgcctg catccccaat cgtaatatag tctgtcttga tttcttccaa
    10141 gaagtctaag aaaggcttga taaggtccat catatcttgg tgcatgagag cattgaccgc
    10201 aacgatcaat tccttaccag catcatgaac caacttagcg atttcacgta attggtcata
    10261 actaaaggtc gttggcagac gaagaccaaa atctttctca ccgacataga tacggtctac
    10321 gccagcttcg agtagttgtt caacttgttc aatactttca gcagttgctg taatgacaat
    10381 cttttccata agaaaaatta taccacattt ctccaaaatc agccatgctt tgaaaatgaa
    10441 aagggacttt attctttttg taacctttct gtaataattc tctgccgaaa aaatgataaa
    10501 ataggtatgt actgttaagg agagaatcat gcccgtaaga aaattacaat cctatgaggt
    10561 agactatcaa gaagaattaa accagcagct tcctcattac caagcttata cacctgaagc
    10621 gcaatctgat gccaatctca aggaaatcct attttttatt aatatcgctg ttttttgtat
    10681 ctgtattgct atctttagtt ttatcttttt agcattaaaa ttatcaactg ctcttgcctt
    10741 tgccgcagca atcggattca gcttacttgt tttaaaagtt caacggtcta ttatcaaacg
    10801 aaaacgtaga agataaatcc aaaatatcgc cccaattgga gcgatatttt tattttttct
    10861 ttttagatgg tgtctgaatg gcaatcttca cttcaaagat tgttcccttg ggtttattat
    10921 ctttgacagt aacagttcct tttagagcat ctacaatttg cttggctagg gataatccta
    10981 aaccaaaacc acctttttgc cgggttctag ccttgtctac tcgataaaaa cggtcaaaaa
    11041 ttttcttttt atcttctgtc gaaataccga ttccattatc agaaacaagt aaataaagat
    11101 tgcgatcggt cgccgagata agaaaatcaa tttcaccatc ctcctcagta tacttgacgg
    11161 cattatcgaa aagaatggtc atcagttgtt tcagaagaag ctgatctgtg acaattgttc
    11221 gatggatacg attttcaaaa cggaagacac gattattttc cgaagcaatc atctcatagt
    11281 ttgtgaaagt tgtattaaaa aagctagttg gaacttctgc aagctccggc ttaatcccat
    11341 catctctccg agctaagttc agcaagcttg tcgttaaaaa acgcatattt cggacttctt
    11401 ccaaactcga tgcaatgctt tcgctcacat ccataatggt agcttctggc ttacgaaaaa
    11461 gggtctctaa gcgattttgc aaaactgcga gtggagttcg taactcatga ctggcatttt
    11521 ccacaaaaga ctgttgcttc tgcatactct caagcagggg cctaacactg accctagcta
    11581 gatagagact ggcaagtaaa gacaaaatcc agaaactagc catcacgacc acaatcaatt
    11641 gctcatgctt ttgactagcc tgctccaact gactggtatt aatcaagaca gcagcatact
    11701 tgatattggt tgaaaccgaa ctaatattgg tctccatcaa aatcacacga tagatttctt
    11761 cctgcccata gctattaaaa acctgaatct ggtagatatg tcctagttct ttcttttcta
    11821 acttaatctt atccaagccc aaaaatcgat ttccagaaag aagttgagta aagtctttat
    11881 caaagagaat gacttccgta ttggaactga cattaggttt tatttctact ttactagcgt
    11941 ccgccctagc attttctaaa tctttaatct cttctgttgc cctatttata gccagctgaa
    12001 taactgcttg aggattttca ctcagtccat gaagcttatc gtccaccgaa gtataaagac
    12061 tcgaatgcat gacttgtaaa ataatcagag tcattgtaga aaaaatcagg gtgaagacac
    12121 cgaagttgcg gataaaataa ctaaagtcat ccgcatacca tgttttttta agtttactga
    12181 acatctttta aaagataccc aacactacgc aaagtttgca aattctctgc aaaagtggtt
    12241 ccctttaatt tcttacggac ttttgaaaca tagacttcga caaccgaaat cgttgtatca
    12301 ctatcaaatc cccatagacg gtcaaaaatc tgcgtcttag gcaaaatcac attttgattt
    12361 tgaaggaaat aaactagtaa gtcgaactct ttccccagca attcgacagg agtatcttca
    12421 actttaacgg tattggttga taaattaacc acgatattcc cataagtcaa ggtgttttca
    12481 ttaaacttcc ctgaacgttt gagaagggcc tgaatccgca ttttaagttc ttctaggtag
    12541 aaaggtttgg tcagataatc atccgctccc agttcaaatc catgtccctt gtcatccaaa
    12601 ctttccttgg cagtcataat cagaactggt gtcgtaattc ccttttcacg caattctttt
    12661 aagacttgga aaccattttt ttctggcaac atcaaatcca gcaaaatcaa gtcatagaca
    12721 ccactctcag cttcgtagag accttcttct ccatcaaata cctgcataac atccgcaaaa
    12781 tcgtctaaaa agtcaaatac tgaatttgac agacctaggt catcctcaac caataagatt
    12841 tttatcatga gaaactcctc cttattaaaa ctattatacc aaatttgcct taaaaaaaac
    12901 tcaactctct gcattttaca tgagatagct gagttttctt tttattttag gcttatttat
    12961 gcatttccgt attgaagaac aactgcttcg actgcagctt tttcacggct aatcaagtca
    13021 acacgtgctg caatttcctt gattcccata ccgatgttac ggctaagagc aaggtcagaa
    13081 agttgcggct caaagaactc cttgtattcc gccaagcgtt gctgagtctt aaatacatga
    13141 gcaggaagga taacaaagct atcaaagctc atatctcctc caagggctgc cttaatccaa
    13201 gcccagtttt cacgcgccca agaccaagct gttttctgag ttgcttgatg agctaggaat
    13261 tggtaatacc aagcagacaa gtcctgtggt ttgaccacaa atttgtcctt ccaagaagta
    13321 atcaggtttt ggatattatc cgcatctgta ctgtatgcaa gagctgctgc caactggcgt
    13381 ttaaagacag catctgttgc gtgagtataa gtatcaagat aaagtgctaa caagtcttta
    13441 gtctcatgat gtttcatctc attaatcaga acttgtgagc gaatagctgc tgggagtcct
    13501 gcaagattct ccttgtgtgt tgcgaagatt tggctagcga cttgactagc ttctgcatca
    13561 tttgagcgaa tcatcatcga aacagccagc tgacgaacca attcatcctc atctgattct
    13621 ccgtctttag cttcaaaacc aagacggtca tagttatgac gagccaattt agcaaccagt
    13681 cctttgaagg ctgtttcagc atccgttcct tcatcaataa agcgctcaag ggctgaaatc
    13741 acttgagaaa cagctgaaac caccagataa gactcttcct tagcaagttt atcaagaact
    13801 ggaagcaagt ctgcataaga aatgtgccct gcctcagcca acaaacgacg ttcttgaaca
    13861 atttgcagtt tgcttgtgtt atcaagtgtc tctagctcag caagaacagc ttctaacaag
    13921 tctccttgat agtcggtaat atagtgggca gtattttcag tgttgagacg aagagctcct
    13981 tcattttcag caagaagagc tgcgtagcca gggatttcga tactttcagt ttcgagtgta
    14041 tcaggcaagc ctttccagtt gctattgagg ggcaccaccc agagacggtt cttgtcttcg
    14101 ttctcaccga tgaagaattg tttttgtgaa atcttcaaga catcattttc aactttgaca
    14161 gtaagaactg ggtaaccagg ctgttccaac caagaatcca tgaaggctgc gacatcacgt
    14221 cctgacgctt gaccaagggc atcccaaagg tcactaccaa tggtgttgct gtattggtgt
    14281 ttttcaaagt aggcgtgcaa acctttagca aaatcagcat ctcctagcca acggcgaagc
    14341 atgtgcatga gacggcttcc tttggcatag acgatagcgc cgtcaaagag tgtattgatt
    14401 tcatctggat gtttaacttc gacgtggaca gactgaacgc catcagtagc gtcacgttca
    14461 agagcaagag gtactccacc tgtttggaaa tcttcaaaga tattccagct tggttcgatg
    14521 gtatccacac agacgtattc catcatatta gcgaaacttt cattgagcca aaggtcatcc
    14581 caccatttca tagtcacgag gttcccaaac cattggtgag ccaattcatg ggccacaaca
    14641 agggcaactt gttgacggct agcaaatgta gagttctcat cgacaaccaa gtaaacttca
    14701 cggtaggtca caagacccca gttttccata gcaccagctg agaagtcagg aagggcgatg
    14761 tggagagatt gaggaattgg gtacttaact ccatagtaat cttcgtaaaa ctcgatagag
    14821 cgaacagcga tatccagtga gaaatcaaga tttgaaagtg gatgtgcttt ggttgagtag
    14881 acacctacca gggtaccatt tttagtttta gcggtcaccc cttgcaaatc accagcaaca
    14941 aaggccaaca agtaagaaga catgcgaggt gttgtctcaa acttccagat acctgtttcc
    15001 ttacggtttt caacatcgat ttctggcatg tttgacaagg ccaattcacc ttctgcttgg
    15061 tcaaagcgaa gagagaggtc aaaagttgct ttggcttcag gctcatccac acatgggaaa
    15121 gcttcgcgcg caaaatggct ctcgaactga gtagacaaga cctccttctt gactccatca
    15181 actgtataat aagaagggta aatccctgtc atgttgtctg taattttacc agaaaaggca
    15241 agaaccaatt caacttgacc agcctcagcc aattcgatat gaagggcttc attgtcatgg
    15301 tcaactgtaa atggacgagc ttgacctgca acttctacag aggtgatttc caagtctttt
    15361 tggtggaggg agatgcggtc actctgtgct tgaccagtga tggtcacttt cccagaaaaa
    15421 gtcttggtct cacgactcaa atctaaaaat aaatcataat gttcaggaac aaattgctta
    15481 ataaaatgtt caactgcttg catagttttc tcctattcta agtttaagag ctgctgctct
    15541 tcttcaaaca aacttattat atcatatttt gcttgagaaa aaaggattgg acagcgattt
    15601 ccaaaacttt cttccttcta atagtctaca gtgggtagac cttgcgaaat tcttctaaaa
    15661 caaccttgct ttcaggtgta aaagtcagcc ctacttcacc caaacagtct gtgaaaaagt
    15721 cctcatgaac ctggcgccaa taggcaagtg atttgtctcc ctcaccttcc ttaaaggcat
    15781 gttgcgcaga aacttgattg aagagttcaa cagaaacctt tgtaatttcg acaacgcaga
    15841 cagcctgatt ttgactatct aaaatgacat cgaaggtccc tacttgggga agtggttcgt
    15901 cttctagtac atagaggtca taggctgatg ctgttgctgt cttttctcct ttaaacacca
    15961 aatccgctaa aaggtctggt tcaactccaa aagcccaagc atcgatttca tctccgatca
    16021 aaggattgat ttgcttgtat ttattccaca tttcttgcgg tatcatgggt actcctttgt
    16081 aattttttac tttcttcttt tatgtgttta agatgatctg gatggtcaat ctctaaatca
    16141 aaaatctctg gaatagaact gtagtggata atgcacttga tacccaactg attcattttt
    16201 tgtatgaaag aagtattcag atagcctgct acagcaaaat taatcttgtt ctttcttgct
    16261 ttatcctgca tatctcttag catatctaac attattggac tttccatatc atgccattga
    16321 ctgtttctca tagtcgcaaa aacaaaggaa gtcaaatcat tcattccaac tacaatcttt
    16381 gaaatgcccg tttccagtat actagataag tcaaaatacg ctgacggtaa ttcaatcatc
    16441 gttccgactt tcccagtaaa gccatactga cgcaatactg taatagcttg ttttaattgg
    16501 tcggcatcat tgacaaaagg aaaaataaga gacagattgg gattggtttg ataaacttct
    16561 gtaacgacat gtgattcagc ctgaaattca tccaaacacg ccagtaaacg cctggttcct
    16621 ctatagccaa acaagggatg cccttcgtca aaaaactctc tagtccccgc taaacaattg
    16681 gcttctgtat tcgttaattc agaaaaacga taccaaactt ccttatctaa gtaaaaggag
    16741 caaatagtat caagataatc ttttacaaat tgctgacaac ttgctaatat gatgttttga
    16801 ttaagctctc tcaataagta ttccccacga atcatgccga cgtggtgaaa tagttgagga
    16861 taaatttttt caagaatttt ttcgccacta agggcaagtt gatttctcat agccattcac
    16921 ctcgtcttaa aagatacaat atcatatata attatatcaa aatttcctct agttcaaaag
    16981 cttcataagg ttcagataac tcatatccta aaaattctgc tacttttttg gaagcctcat
    17041 tatgagcatc ccaaagtgga aacatatctc tatttaaact ctctagaatc attgcagcac
    17101 caagcttttt agcaaatcca tttccttgtt cgtttggtct agttgcaact tccacttcaa
    17161 ctgctttacg gtaaactaac cctgaggaaa tcccagcaat cagttcatta tttttaagaa
    17221 tcacaaagcc aaatccacct tttaaaacaa aatcctgata ggactcaaaa tccccctgta
    17281 aatcctgtga ccattcttcc gtagaaaagc agttataaat atgattatca ataggcacaa
    17341 tattgtaacc ttcctctaaa tgagtaacta gattatttaa aaattcaact tgaaaatttg
    17401 ccttatcttt aaaagaatag cgagtaaaag aatttagccc cacttttcga tgcagaaaat
    17461 cttgccaatt agtattttct gaaataataa tcttataatc taaaccatat tttctaacaa
    17521 aatcttccca aaatctggaa tccaactccc ctgctaggta tagaaaattc ccaacatcat
    17581 aaaagcttgt tgtcctatca ttgtttcggt agacagttcc aatttgagat ttaagaccat
    17641 agagaaccat atttttcttc catgttttaa atagttccat tatccctcta cttcaccttc
    17701 caattcatgt aagaagtctt gtccagttct ggaaatccta ataattcaga cttaaccttc
    17761 aagactaatg gcgatgcatt ttcttctgta atctcttgaa tatccatcca aatatatcca
    17821 agtgaatcat tcgcaccatc agacacagct tccgaagtcg taacttgagg tgcactctca
    17881 ttcatttcaa catcatacaa ggccatgaca tggtgaacca taaaatttgt taactcttct
    17941 ctgacgaaaa catcgtagat tcgaggatta gagtagcttc taacagtaaa tcccgtctct
    18001 tccataactt ctctagtcag cgtttccgtc agtccttcac caagttgctg actgcctcca
    18061 ggtagatcat accgatgttg ataagggcct ctcgcttttt caatgcagag taacttccca
    18121 ttttctaagc aaacagcgta gactccaaag tgttttttga tttccatcca actcctccta
    18181 cttcaaagac cagccaccat ctattgtcaa gatttgtcct tgcatggcgc tcgcttttcc
    18241 acttgctaaa aaaagactaa gctctgctat ttcctctggc tcaatccagc gcttgattgg
    18301 ggtttcacta gccacccagt cagccaaacc acctggttca aaatccgcag cggtcatagc
    18361 tgtcttgact gctcctggag cgatcccaaa gacctgaatc ccagcttcag catagtctag
    18421 agccaactgc ttggtgaagc cagccaaggc atgcttggat gaagtatagg cgtgaccacc
    18481 tccacctgct aggctagaag caatggaaca catattgatg atgattccct ttttattttc
    18541 cagcatttgt gtcaaataat accgagtcaa ctctactgga ataatgtagt tgatttcaaa
    18601 aatctcttga atgtcctgcg ccgtttgttc caacagtggt ttgtaatcat ccaaaactcc
    18661 ggcagtatta cacaaaacat ccacctgagg gcaccagtca aaaataggtt ccaagtccaa
    18721 ggtcaaatct ctctgtaaaa agtgaaaatc accctctaag agtggctttt caccttggtc
    18781 aactccataa acttgatagc ccttctctaa aaagaggcga gcttgagcca atccgatccc
    18841 tgaactcact cctgtaatga gtacacgttt agtcatgcac ttctacccaa tccgttgcca
    18901 aaacatcaca agctgtcggg ctccacatgg aaaaaccttc tccttctcca gacacgttga
    18961 ttaggaaata aggtgtcatt tcaagtgcaa gcccattttg ctcgatggta tcaaagagtt
    19021 ggacatagtt ttccgcacct ccccaaccag ttcgtacata ttttctctta gcctttaacc
    19081 caggcaggat ctcttcaaat gtcatgtttt tctcctttaa ttctacattc ttcatttaat
    19141 tataacaaaa aaaccgcttt aaaacggctt tttgactgtg atttatttac atcttcttct
    19201 acttacggca aattattccc tgcagcaaga taaatttcat accattcttt tcttgttaag
    19261 ctaaagtttg ccgcttggct aacttctctc aagtgcttag gatttgtggt acctacaact
    19321 gcctgcattt ttgctggata acgcaatatc caagaaatgg caatagttga agaggttact
    19381 ccatatttaa tagctaaacg atcaagtact tgatttaaag cttgaaattt ctcatttcca
    19441 acaaaattcc ctttaaaata cccgaattgt aagacagacc atgcttgaat gaccacatcg
    19501 tgtaatttgc aatattcaaa aatgctgcca tctcgcatag ctgcttgact atcttccata
    19561 ttaacatgaa aagctgattc aaatcctgga gtaaaagccg cactcaattg tagctgatta
    19621 acagctaacg gctgcttgac atctttttta agcaactcca tcatcatagg attttgatta
    19681 gaaactccaa aatctcgaac tttaccttgt ttataaagga gatcaaaggc ttctgctact
    19741 tggtcagatt ccatcaaagc atctggtcga tgaaggagca agctatctag atgatcaatc
    19801 ttcaatcttt gcaaaatacc gtctactgat tttataatat agtccttaga aaaatcaaaa
    19861 taggtaaatt cttcaatgcg aatgccacat ttggactgaa tccacatctt ttctcttaaa
    19921 tctggacgat tttttaggac aagacctaac agttcttcac aacgaccacg accataaata
    19981 tcagccaagt cgaaggcatt gattccaaca gaaagtgctg tttctacaag ctcttcaact
    20041 tcttttacag acttatcttt tattctcatc attccgagaa caatttctga taattctttg
    20101 tcatcttgac caagagttat gtatctcatc aaatttttct cctttaattt ctaacattct
    20161 tcccttcatt ataacaaaaa accgctttgc aacggctttt tgactatact tcactccatt
    20221 ttatcttctt aaacccacgg aacaagacaa agattccaat aaagaggaca gctaaaggaa
    20281 taacttttgt aaggaaaaca tttgaaattc ccatccactc ataataacgg agcagagaac
    20341 ccaccacaag atgggcaata atcatactga caaatggacg aaagaccgct tctttccaat
    20401 tccaaatacc gataactagc gaaatcgtaa agacagacaa actatcccag ggagccggaa
    20461 tataaaaggc tccttcttgt atgaagcttg ccattcctac atatcctaaa acaactagaa
    20521 gaactatagt cccaacaaca atgtaagtgc caattttcat tttaggagaa tcttggacta
    20581 aacttcttcg taaaattgtg accacaagtc caaatccaat cagaaaaata agaagttgcc
    20641 ctaaaaatgt gagcaaattg actgttaaga gaggaccttt agaaaaatca cttagtagtt
    20701 gataataacg taataccgcc aggacaagaa ttggcgtcaa aagggactct ttgatagaac
    20761 tgcgaggtgc tcccttgaga atctctttca ttattttttt aggattctta cctagataat
    20821 cctctgcact catgccatct cgttctgctt ctgagaaatc tagcatcatc aaatagatct
    20881 gctctctgag atagtcttca tcatagagaa atccaacaag attaaaactt tcccacaact
    20941 cctcaaaata cttttgattc tcctcagaaa actcatgtag caaagcgctt gtttcttcgt
    21001 aatacttcat tttcttcatg gtttaacccc cattcttaat cccttctact ttttgactca
    21061 aatcgtccca ttgttgccaa aagactgaga cacgctcttc tccttctttc attaatgaaa
    21121 aatacttccg atctggacca tctggcgacg ggcgcatgtc gcctcttatc cattgatttt
    21181 tttctaactt ttgcaacaaa ggataaatag ttcctggaac gatagtatca aatccagcct
    21241 ctcgcaaagt ctgaaccaac tcataaccat accgctcttt ttgaccaatc atatccaaga
    21301 cacaaccttc aagaacacct tttaatagct gagtttcttt catcacttct cccttctaat
    21361 ctattttgta atacctacta gtaacttcac ctatagtata tcacttctac actagtttgt
    21421 aaagcataat agttaatact cttcgaaaat ctcttcaaac cacgtcatcg tcgccctacc
    21481 gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaaacat gttttgagct
    21541 gacttcgtca gtttcatcta caacctcaaa aacatgtttt gagctgactt cgtcagttt
//
LOCUS       DAVDWM010000036        20388 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVDWM010000036 DAVDWM010000000
VERSION     DAVDWM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.036"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..422
                     /locus_tag="VUW49_001538"
     CDS             <1..422
                     /locus_tag="VUW49_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981435.1"
                     /translation="NHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNYSHDMKLFKMSCR
                     NIGQAGKILADSDYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHALSKERSKVENIF
                     AKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(411..1058)
                     /locus_tag="VUW49_001539"
     CDS             complement(411..1058)
                     /locus_tag="VUW49_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET7981436.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(1167..1685)
                     /locus_tag="VUW49_001540"
     CDS             complement(1167..1685)
                     /locus_tag="VUW49_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET7981437.1"
                     /translation="MRTYENKEELKAEIEKTFEKYISEFDNIPENLKDKRVDEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1912..2781)
                     /gene="rsmI"
                     /locus_tag="VUW49_001541"
     CDS             complement(1912..2781)
                     /gene="rsmI"
                     /locus_tag="VUW49_001541"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7981438.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQILKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2784..3101)
                     /gene="yabA"
                     /locus_tag="VUW49_001542"
     CDS             complement(2784..3101)
                     /gene="yabA"
                     /locus_tag="VUW49_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET7981439.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(3141..4031)
                     /locus_tag="VUW49_001543"
     CDS             complement(3141..4031)
                     /locus_tag="VUW49_001543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7981440.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(4028..4666)
                     /locus_tag="VUW49_001544"
     CDS             complement(4028..4666)
                     /locus_tag="VUW49_001544"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7981441.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4885..5682)
                     /gene="proC"
                     /locus_tag="VUW49_001545"
     CDS             complement(4885..5682)
                     /gene="proC"
                     /locus_tag="VUW49_001545"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7981442.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(5686..6948)
                     /locus_tag="VUW49_001546"
     CDS             complement(5686..6948)
                     /locus_tag="VUW49_001546"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET7981443.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6958..8088)
                     /locus_tag="VUW49_001547"
     CDS             complement(6958..8088)
                     /locus_tag="VUW49_001547"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET7981444.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(8168..10051)
                     /gene="cbpE"
                     /locus_tag="VUW49_001548"
                     /gene_synonym="pce"
     CDS             complement(8168..10051)
                     /gene="cbpE"
                     /locus_tag="VUW49_001548"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET7981445.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTISYSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(10054..10941)
                     /locus_tag="VUW49_001549"
     CDS             complement(10054..10941)
                     /locus_tag="VUW49_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7981446.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10931..11392)
                     /locus_tag="VUW49_001550"
     CDS             complement(10931..11392)
                     /locus_tag="VUW49_001550"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7981447.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(11389..12297)
                     /locus_tag="VUW49_001551"
     CDS             complement(11389..12297)
                     /locus_tag="VUW49_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7981448.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            12290..12487
                     /locus_tag="VUW49_001552"
                     /pseudo
     CDS             12290..12487
                     /locus_tag="VUW49_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(12539..13210)
                     /locus_tag="VUW49_001553"
     CDS             complement(12539..13210)
                     /locus_tag="VUW49_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7981449.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLLESGVLLSAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVIMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(13264..13485)
                     /locus_tag="VUW49_001554"
     CDS             complement(13264..13485)
                     /locus_tag="VUW49_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981450.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWQMEPVLTMP
                     VHGFCPRGTDDLGYIVGGNHLCKGSAARF"
     gene            13757..14566
                     /locus_tag="VUW49_001555"
     CDS             13757..14566
                     /locus_tag="VUW49_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7981451.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKINSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14747..15622)
                     /gene="aguB"
                     /locus_tag="VUW49_001556"
     CDS             complement(14747..15622)
                     /gene="aguB"
                     /locus_tag="VUW49_001556"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET7981452.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15632..16717)
                     /gene="aguA"
                     /locus_tag="VUW49_001557"
     CDS             complement(15632..16717)
                     /gene="aguA"
                     /locus_tag="VUW49_001557"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET7981453.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16714..17841)
                     /gene="nspC"
                     /locus_tag="VUW49_001558"
     CDS             complement(16714..17841)
                     /gene="nspC"
                     /locus_tag="VUW49_001558"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET7981454.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKEVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17841..19100)
                     /locus_tag="VUW49_001559"
     CDS             complement(17841..19100)
                     /locus_tag="VUW49_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET7981455.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(19097..19957)
                     /gene="speE"
                     /locus_tag="VUW49_001560"
     CDS             complement(19097..19957)
                     /gene="speE"
                     /locus_tag="VUW49_001560"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET7981456.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            20213..>20388
                     /locus_tag="VUW49_001561"
     CDS             20213..>20388
                     /locus_tag="VUW49_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981457.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENT"
BASE COUNT         5923 a         4667 c         3655 g         6143 t
ORIGIN      
        1 cgaatcattc tggtaaaaag aaatttcacg ctatgaaggc tcaagcgatt gtcacaagtc
       61 aagggagaat tgtttctttg gatatcgctg tgaactatag tcatgatatg aagttgttca
      121 aaatgagttg cagaaatatc ggacaagctg gtaaaatctt ggctgacagt gattatcaag
      181 ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa ctcaagccgc
      241 taacagctga agataaagcc tgtaaccatg cgctatctaa ggagagaagc aaggttgaga
      301 acatctttgc caaagtaaaa acgtttaaaa tgttttcaac aacctatcga aatcatcgta
      361 aacgcttcgg attacgaatg aatttgattg ctggtattat caatcatgaa ctaggattct
      421 agttttgcag gaagtctaat gtaattctca agctgtgagt tcattatgat ttgtaaatcc
      481 gatatatctt cagcagctaa aaacacattt tgaaatgttc caagtccgtt ttcaccaaaa
      541 ctatattctc tcttactata cttactctta ttattctcta tatagttaga gtcaaggttc
      601 ttaacctctt gaagttcatt ttctttactt ctgaggttaa aatcttttac ttctgaagtt
      661 aagttttttg acttctgtaa gtcattttct tttacctgaa atatactcat aaagtcttta
      721 acataaatga tgttcggctt accaagtcca agccttactc tttcgatcag tcctattcct
      781 ttttttacat caagctcatc taatgttttt atggcagttg gctttgagat atttcttctt
      841 ttcataattt cttcgacagt aaaatagata aatactctgc cttccttgtc tatccagtta
      901 ttcttaaatg acattcctgt ctgtttaaga agtatggaat aaaggataat tgcttctgct
      961 gataagcccc gaaattcttc tctatcaact aatatctccg gtacttttaa gaagttaaat
     1021 ctttccgctt ctctgttata aaaatattca aagtccatgc aagatcacct cccttcttaa
     1081 aaatttgcaa agaaaagacg atagtttttt cttctatcga ctttggtaac catttttatg
     1141 aaatttttta aagttaaaaa tataatttat aatactatct tcttccattt tctgatttta
     1201 gttctgaaag ttccaaaagg tgcaaccgta tttacatgaa taaacttata cacttcccaa
     1261 gtcgctgttt tagtcgcttc atcagcccac tttctcatat gcggttcaaa taattcttcc
     1321 tcactcaacg aatcaatcat tgcagagata gaatgaatat tttcatttaa ttttgctttc
     1381 aactcttgca gagataaatg agcgtaggta tccgtgaacc actgatataa ttcaccaagt
     1441 tgattccatt taaatttatc cgatggtgtt tttacttgaa gcccctttct ttcatcttct
     1501 tcccatttaa gaaccaagtt ggtccagcca acctgataag caaggttttc tgctggagtt
     1561 ctatcaactt catcaactct cttatctttt aaattttctg gaatattatc aaattctgaa
     1621 atatatttct caaatgtttt ctctatctca gcttttagtt cttctttatt ttcgtatgtt
     1681 cgcaaaaaat caccttcttt ctgatttatc aatcctttga tttaaaatga ccttattttt
     1741 ctatttatac cttctaagaa cgctataact gctgctccaa ccatcggtat tccgtatggg
     1801 cttaataaaa aacttaatac caaagcttct ataccaagtg atacatcttt atattatcat
     1861 cactaattaa gttaaacaga aacagacaga attattacat cctgtctccc tttattgttt
     1921 ttcttcccag tcgtggtagg cagcgtagag ctgactttta ttccactggt aaatcttagc
     1981 gacttccttg atagcttggt ttttcttcac accttgctgg atgcgggttt gaatttctac
     2041 gaacaagtct tcctcgtcct tttcctccac accctgactg gcaccctcaa caatgagaag
     2101 acattcgccc ttgagtggcg tttcagcaat gctttctaat aactcagaga tagtacctcg
     2161 ttggtattct tcatagattt tggtcaattc cctgaccaag acaacggagc ggtcaccgta
     2221 gacttctaac atattttcca acgtgtctgc tacacgatga ggtgattcat aaaaaatctg
     2281 agtttcagga tagtcttttt tcgagtcgaa aaattgcttt tgctggcctg attttctcgg
     2341 caaaaagccg taaaagatat gtggctgcgg cgctaaacca ctggcaatca aggcagaaat
     2401 tcctgcagag gcacctggaa ctgtcacaac tgcaatttct tcctcaatag ctgccttaac
     2461 caaatcatga ccagggtctg aaatgctagg caaaccggca tcagagacct gagcaatact
     2521 ttgccctgct ttcaagaaac caatcaaatc aggaattttt tccttggcat tgtgctcatg
     2581 aaaactgatc tgcttggtgg aaatgtcaaa atgcttgagc aaaagacctg tattgcgcgt
     2641 atcctcagca gcaatccagt ccacttcttt caagatctgg atagcacgaa aagtcatatc
     2701 atctagattg ccaatcggcg ttgccactag atacagtttg ccatagggag actgcccctt
     2761 aaaacttttt tgaatttgca tgcttactcc ctgtataaca actcatcaca gaacatacat
     2821 tcttcgtcct gctctcgacg ttgtccataa aaatcattac atacgtgaaa tccatcacgg
     2881 taaatacgac ggacactttc gcgaacatgc ttggccttga cgggagcgtc tgcttccacc
     2941 tcacccaagc gttctcgcaa cttactattt tccaagcgaa gagctgtatt ttcttctacc
     3001 aagctcttga gatttttctt gatggcttcc acatcggcta aggttaccaa taattgttgg
     3061 gaaaaatcat ccagcgcgtc aaataattct tttttgtcca taaaacagcc ctttcctttc
     3121 tttatcattc atttttgagt ttatatttct ttcaagacca gatattccat ggcattttga
     3181 aagctgacat tagcttgcca catttttcta gcttctagta aatcttgtag aatcactctt
     3241 actcttacct gcaagaggtc ctgcccgcag agaacttcaa gaatccgtaa aacctgatcc
     3301 tgtttttcct tatcatctgc caagttggct aatttggcaa cctgtagata actttctttt
     3361 ttcttagcta ctaaccaagt cagcaggcgt tcactttcat cgaccaaggt ccaaaaactt
     3421 gcctgattag ccaacttttc tgcttcagct cgcgattgac taaacttagc taaaagagtc
     3481 gcttttttct taacaagtcc catttgttct aagagtaaga taagtttttc ttcttgcttt
     3541 ttaaagtgga agatctgagt ccgacttcgg attgtcggta acatcttttc ctcatcgcta
     3601 gtcaagaaga aaatataaac ttcactctgg ggttcttcga tgaccttgag cagagaattg
     3661 gctgcgttgg gatgcatttt atccgcttgc tcgataataa agacctgttg ctggctttca
     3721 atccctgctt gagaaaactg tcccaccaat tccctaatgc gttctgtctt gatgacctga
     3781 ttgactggct taatcaaggt gacatcggga aattctccct gttcaatcag cttgcaactt
     3841 cggcatttct cacatggtaa gacgccaact ttatccgtac aaaagaggct cttagctaaa
     3901 aattgcgcta tttccaagct tccaaagaaa cctgaaaaga gataggcgtg attgagctgg
     3961 tcttgttcta agatacggac aaaacggtca aactgagctg gttgccaagc ctttagttga
     4021 tcttgtttca tttggccaag cccattccgt caaacaagac agccttggta gtttccacaa
     4081 cttgctccaa agggagacta gcatcaatct tgacaatgcg atttccctct ttatccagaa
     4141 gagaaaggta gccttgacga acttttttat gcaagtccaa cccttccaaa tccaaacgat
     4201 taacctcgcg gtcactatta gcagcaatac gagccagccc ttcttccacc tcgatgtcaa
     4261 aatagagtgt caaatcgggt ttgaggccat ctgtcgcaaa ctgattgagc cagtcaatgg
     4321 cttcaatatc taagccacga ccaaatccct gataggcaac agaactatcg ataaaacgat
     4381 ccatgatgac caacttgcca gcttcaaggg ctggaagaac tttttccacc aaatgctgtc
     4441 tgcgactggc aatatagaga agtagctctg ttttagcatc catctgagta tgacttggat
     4501 ccaaaatcac ttcccgaatc ttctccccaa tcaagactcc gccaggttca cgggtcgtca
     4561 acacctctac tcctttttcc tctaaaattg gtagcagagc ctctaaaaca ctggtcttgc
     4621 ctgctccctc tggtccctca agagagacta aaaatccttt tgacatgtct aactcatttc
     4681 ttttttacta cttctattct atcaaaaaaa taggggtttg tgacaatctt tttgtattct
     4741 catttcatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gctgtacttg
     4801 agtacggcaa ggtgaagctg acgcagtttg aagagatttt cgaagagtat caaccaccat
     4861 aaaagaggca gataaaacta cctcttattt acctagttct tgtgttcgtt tgtaggcttg
     4921 atgaactgca tccatgactg ttcctcggaa agcatgcgct tctaggcttg ctacaccagc
     4981 gatagtcgaa ccgcctgggc tacagacttg gtctttcaat actccaggat gttgctgact
     5041 ttcaaggacc aattgcccag ctcctaccac agtttgtgct gccattttca atgctatttc
     5101 tcgtggtaat cctgtctgaa cacctgcatc tgccaaggcc tcgataaaaa gatagacaaa
     5161 ggctggtcca cagcctgcaa gacctgtcgc tgcattgatt aagctttctc ctaattcaac
     5221 caagagacca gccttggcta aaagctgaca aaagagctca ctgtcctcag ccctgcaatt
     5281 aggagacaag gcataactaa tcactccttg gccgatagaa gcaggggtat taggcatcat
     5341 acgaataatt cggtgttggc ttgggataag acttgctagt ttttctaagg tcaatccagc
     5401 tgccatcgaa atcaaaagaa gactttctct tttttcaagg atggtctggt attgagaaag
     5461 cagttcagaa aactgagcag gcttaacacc tagaaaaatc acatctgctt cttcgaatat
     5521 ttcttcattg ttagaagtct gaccaccgaa gttagcgatg aaagcatcca ccttgacttg
     5581 actacgattg gcaaggagaa tctcatctga cggtctagcc tgcaagacag acttggctaa
     5641 actagcaccc atattcccca aaccgataaa tccaatcttc atctcttact cccttatctg
     5701 tccatcacca gtgaccacat acttgtagct agtcaattcc ttcaagccca tgggaccacg
     5761 cgcgtgcaat ttctgagtag aaatccccat ttcacaacca agaccaaatt gtcctccatc
     5821 tgtgaaacga gttgaggcat taacatacac cgctgcagag tccacttgat ctgtaaagta
     5881 tgctgcagct tcagcatttt ccgtcacaat ggcatccgaa tgatgggtac tgtgggattc
     5941 aatatgcgca accgcttctt ctaaactgct cacaacctta acagctagaa tatagtctaa
     6001 aaactcggtg tcaaagtctt gggtctcagc tgcttgacct gaaacaaact ggcttgcttt
     6061 gctatccaag cggaattgaa ttggttccag tccagcttcc ttacgctctg caaccagcat
     6121 ttgatccaag cgaggaagga tgcttgctgc cttgttttca tgaaccagca gaacctccat
     6181 ggcattacaa acagaaggac gactggtttt agcattgttg atgatagaca gcgccttgtc
     6241 ttcgtctgca tccttatcca catagacatg gacaatccca gtccctgtct cgataacagg
     6301 tacaatcgca ttctcaacca ctgcattgat caagccagct cctccacgag gaatgagaag
     6361 gtctagatag cccttggcct tcatcatagc ataactactt tcacggctag tatcctccac
     6421 cagttgaatc acatttggat gaatagtagt cgtctccaag cccttcttca aggctgtgac
     6481 aatggcatgg gttgtttgat aggcatcctt accactacga agaacaaccg catttccact
     6541 cttaagagtc aaagcagccg catcagacgt cacatttgga cggctttcat agataatacc
     6601 gatgacaccc atagctacac gtttttttgt gataaccaaa ccattttcaa gctgacttgt
     6661 ttctaaaact tcaccgattg gatctggtaa ggcaaccact tcacgaattc ctcttgccat
     6721 cgcttctata cgatctgcat ccaaataaag acgatccaac atcacatctg agattttccc
     6781 cttagccgct gccatatcga gggcattagc cgctaaaatt tcctcagtag cagccactaa
     6841 gtgatcagcc atggctagca aggcttggtt cttcacttct tcactagctg tgttaatcga
     6901 ttttttaaca gcctgtacct gttcaaattg ttcttgtcta ctcaccatag tttacctcta
     6961 aaattctgta aaaagtagtt ggatttcagg agtaatggaa atccagtcgt cacggtaaat
     7021 caagacaccc ttggcttttt gagaacgcaa tatatcctcc aaagcagatg ctccaaattg
     7081 cacgcgtcct tttccaagtg attttccact ttccttgtca aatactgtca cgatatcacc
     7141 gtaagaaaag gctccttctg cttcaacgat accagataag agaagactct ttccatgttg
     7201 agagagagct tccgcagccc ctttatcaac ccaaatagaa ccttgactct gagcatagaa
     7261 ggcaagccat tgtttctggg tacgaagccc cttctcttga gcaacaaagt aagaaccatc
     7321 ctcggtctcc tctgccgcct caatcatgga atctgatttc aaggatgagc agatataaac
     7381 aggaactcct gattccgtcg cgatagttgc agccttgatt ttggttaaca tacccccagt
     7441 tccgtttgac gaaccagctc caccagccat atcaataatc tcacgattga tggtctcgat
     7501 tctctccaag cgtttggctc ttggatctga attaggattt ccagtataga gaccgtccac
     7561 atctgtcaag agaaccaaaa ggtctgcttg gaccatcgcc gctacttggg cacttagagt
     7621 gtcattgtcc ccaaccttga cctcatcaat aacgacacta tcattctcat tgatgatagg
     7681 aattgcccca cggttgagca aaaccgacaa agcctgatgg gcatttttat aacgacgctt
     7741 atccacaaag tcatcttggg tcagcaagat ttgtgcagaa acgatttgac gcaagagaag
     7801 attggttgta tattcttcca acaaaagccc ctgccctacc gctgctgaag cctgtttatc
     7861 agcaatttta gtcggacgct ttttaaatcc taaggctcca aaaccagccg caatggcacc
     7921 tgaagacacc aaaatcaact catgaccagc ctcgtgcagc atagccaact gctgggtaat
     7981 atcctttacc ttactacgtg ataaacttcc atcctcattt gtcagagaag aagtacccac
     8041 cttaaagaca atccgtttgt atttcatagt ctcactccga ttattcatat ttatccatta
     8101 taacatgaat aacaggaaat agaaaagagt tgataggaaa aagaaggccc taaaaccttc
     8161 ttttctacta ctgttctgat tccgatttgt ttacactcgt ttcagagctt tgtgaagttg
     8221 aggatgtcaa atcttgacta gttgaaaaat ttgtaacact ttcatgttga ctcgtttctt
     8281 tattttcaag atcctttttc actacttctt tggattcttt tatttctgaa taacttatag
     8341 ttttaattgt agcagatttc tcgatcggaa catccactaa ccaggcacca cttgaatcaa
     8401 cagtatagcc ttctggtgtc ttaccattca caagtaattg accattttct ttcaagaagt
     8461 accatttatc cttgtcttta atccaaccta tagctcgtga accacctgtc ttgtagaaat
     8521 accaatcatt tgccttttta agccagcctt gcttcatagc tcctgattct tctagatagt
     8581 aatgatgtcc agcaacttct atctcacctg tcttcatgat accagtagaa tccatatagt
     8641 accaggcttc tttatctttt acccagcccg ttttcatttc ccctgatgga gcaaagtaat
     8701 accattgccc cttatattga agccaacctg tcttcataga gccatcaaca tccaaataat
     8761 accactgctc tttatcttga atccaaccaa tttccatctg gttttctttg ttaaaataat
     8821 accagattcc agcgattttc ttccaatttt tagcagcaac accagagtct gtcaaataga
     8881 accaacgatt gttccatttt ttccattgat tctgtaataa gatacccgtt tggttaaagt
     8941 aataccattc acccttaatt tcattccaac cgacagcata ttctcctgta gaatcaggcg
     9001 cttgatacca ccagtttcca tatgaactct tatgccaacc agcttgaaaa cttggaatcg
     9061 gcttgtagga agttgaaatg tttactaaac cgtcttgtcg aatatcaaaa actgttgcat
     9121 catagtcttt gctggctgcg ttgattctct caattcctcg ttctttgagc caattaacat
     9181 actcactatc aacaccattt ttccaaggta gactatccga agtttgaaca atcaaactcg
     9241 gactcaaatt tttaatgaaa tccttggtat ttgatttgtt ggtatcatgg tgatgattaa
     9301 acttcatcaa atcaactttt ccaatgagag gaccatactt gtcttctgct ccatgaacat
     9361 tatctaagtc gcccccaagg taaattttct tgccattgac tttcaccacg ctaatcaagg
     9421 aattggaatt atcatcccaa attttcttta attcacccga tgaatcggtt tcattttcat
     9481 agttatagag ctgaatatcc atgtccccaa actgaaaatg agcatcccct tgtgtgatat
     9541 tttgaataac tgaaacacct ttttctgaag cagtctgtaa aaccttatca taaccgtaca
     9601 gattatccca tagacgttca gaattagtaa tacgattatc actatatttt ttaagataga
     9661 ctcggtcaac tggataggta gacagtaatt catcaacatt tccaatatga tcactgtggg
     9721 tatgggtcac caaaataaaa tcaagttttt ggacacccaa ttccttcaaa cgacgaaaga
     9781 cacggtctgt tagaacatgc ttataagacg tttcaattcc ttctctccat ggatagcgag
     9841 aatcacttcc atctgggaaa tcataatctt ctcctgtatc caccatggca aaatgtccat
     9901 tgctttcaag aataatcgca tcactgccac cttcttgaac attgataaag tggattttat
     9961 ttcctgaact ttcttgagcc tgaacattcc cataccatga caaacctaaa aaagcgccta
    10021 caagtgctaa actagttaat ttctttttca ttcttacttt ctcaatctct ccaaggtttc
    10081 cttaaaaatc tctgggatat ctgctttaaa ttccaaggtc ttacctgttc tcggatgagt
    10141 aaaacctaaa gtcttggcat gaagaaattg tccatgtcct ttcaaagtct tgcgaggacc
    10201 atagacctca tcaccagcga ctggatggcc gatataagcc atgtgaacac ggatttgatg
    10261 agtgcgccct gtctccagtt gcaactctac taagctataa tcgccaaagc gttccaagac
    10321 gtgaaaacgc gtcactgcag gcttcccttt agcagttaca gcctgtttct tacggtcttt
    10381 ttcactccgg ccaatcggcg cttcaattac accacgatca ttaggcagat ttccatgaac
    10441 aatcgcccaa tatttgcgga gagacttttt atccttgagt tcttgggcaa gtgctagatg
    10501 cgcatcatcg tttttagcaa tcatgagaag acctgacgta tccttatcaa tacggtgaac
    10561 aatccctgga cgcagaaccc cattgatacc cgacaagtcc ttaatatgat acatgagggc
    10621 atttactagg gttccactgg tatgaccagc actcgggtgc acaaccattc cctgaggttt
    10681 gttaacgaca gccacatcct catcttggta gactatttct agcggaagat cctcagccac
    10741 atactctaat acctctggtt ctggcacatg gtaagtgacg acatcaccct cttggactgt
    10801 gtatttagct ttcttgactt gaccattgac caagacctgg cctgatttaa tttgttcatt
    10861 cgcgagacta cgtgataatt ctgacaaatc tgacaaagcc ttatccaaac gcagaccacc
    10921 agtttcaatt ttaatttcca tttatttcct cttttagcat tgcaatcaat aaaataatca
    10981 ctccaaccgt cagatagcta tctgccacat tgaaaattgc aaagttgata aagtcaaggt
    11041 ggaacatatc cacaacaaag ccctgactga ccctgtcaat aaagtttcca agaccacccg
    11101 cgattattag agtcaaaccc aagaccatcc agaatgagtc ctccatgtgt ttatgtaaat
    11161 accaaatggc acctatcacg acaaccagag taatgacagc gaataacagc tgctgatctt
    11221 gtaagataga aaaggctgca cctcgatttt gcaggtaggt caagctaacg aaattgggga
    11281 tccaggagcg cacttcaccc agtggaatct gctggacgat ataggatttg accaactgat
    11341 ccagcccaat taaaagcagt acaatgactg ccactattgc tctttttttc atgatttcct
    11401 cttttgatca aaatattctt gcatgacttc tacgaagaga gtcccagctt gactaagctc
    11461 cacttcttca cgtttaacat agaccatgcg gttatctagg ttatccttga gacgaataac
    11521 tgtaatgcca ttaacactgt cactatctaa aaatccagaa cctgtcgcat aggcgtccgt
    11581 ccgctccaaa ataccattca aggtggcacg gtctgtcaca ttaaacatct gtgagctagc
    11641 gctggtatcg acaaagttct ctgaataata aaggtactcg tctttctctt gagtgaaacg
    11701 aaccgttggt aaatccgcta aatcctccat gactaattcc tctttctggg ctaaaggatg
    11761 accctcacgg agataaatat gggtatggaa gggaatcaac tcaatgacct ccagacctaa
    11821 tttttcaacc cgttgcataa tccccttttt attttgattg ttgaggtaga taatcccaat
    11881 ctcactatgc ccttgcgcca cttcatctaa tatttgaaca gtagttgatt caaaaatacg
    11941 gaagttctta tagtcaggat agcgctctga aaaggccgta atagttggtg gcaagaagtc
    12001 atagtgctgg ctagcaacgg aaaattcatc tttttcttct tcaggattgg catactgatt
    12061 ttgaaaaata tcaaatcctt taaccaattc ttgcgctttt tcataaaatt ccatcccacg
    12121 acgggtcaag aaagtccctg agctggtccg acggaaaatc ttaaaaccca actctttttc
    12181 caaatcccga acagaaatag acagactcgg ctgactaaca tacatctttt cagcagcttc
    12241 acgaaaagta ccactattgg caatagccac aacatagcgt aattgttgaa tgttcatctt
    12301 ctacccctaa cttctttatc ttttcattat accatatttt agaagttttc caaaaaggaa
    12361 aaaagaacat cctattcttc ttaactatct tcactatctg cctttctcaa gccaacctta
    12421 ttttcaaaat caattcaaaa aaataagtgg ctacacacca ctacaatatc gaaaagaaaa
    12481 acgttgacta tttgtaaaaa aagaaaatgc cggaaagttc cgacattatt ttagtaagct
    12541 aacttcctga aaataggagc aaccacacat cccagaaggc agtgatatta atgagaatat
    12601 gaactagtat tggatagtag agatttttag tcatgcgata caatagagct aaaataagac
    12661 ctccactagc ataaatgaaa aaagcaagag gagttaaagc aaaattgata tgaatataac
    12721 cgaaaataat agcagaaagc aaaacatctc cgtaccaagg cgagtttttg aaaaaggttg
    12781 tcataatcac accgcgataa atcaattcct cagcaatagg ggcgatgaag caaacgatga
    12841 gcaagaaata agggaactcc tgtcgtccca tcatttctat cgtttcattc aaagaaattt
    12901 gatttgaaga cagggatatg aaatacgaaa agagaaagtc agacatatac gaaatgatgt
    12961 agcccagtaa aaggtagata aagtacctca actgccactt gaaatgaaaa atcttctttc
    13021 ctgcaaaaac tagtagatag atgactgctg acaaaagaac accactttcc agcaaaagta
    13081 gaatctgaaa aaattcacga cttgctggta gatagggctt tgcgagataa ttgaaaagcc
    13141 tccaaaacca agttgatttg taaaaaatta aggacaatgc tagtaaaatt tgaatagctc
    13201 gttttttcat attaaatcct ctgttttctc cccttgtttc ttcatattcc taataaattt
    13261 tacttaaaat ctcgcagcac ttcctttgca aagatgatta cctcctacaa tatatcctaa
    13321 atcatcggtt cctctgggac aaaatccatg aaccggcata gttaatactg gttccatttg
    13381 ccaaggataa taaccattag gttctttacc atagggatct gctccacctt gtatcagctc
    13441 taattcttca tttgttagat ttatattttt tgtcattaaa atcatgatat atttcctccg
    13501 ttattgttta gattagttga gtaatctgat actcatacct acttacaaaa aaactattat
    13561 attaagttgg ttttttaatt tgtctagttg caacattgac aaacatagta tagcatatct
    13621 ttgccaaata tcctcttcaa atcatgaatt gtcatcaaaa catcttaaac tataaaatca
    13681 attagtctca agctttctat caatttcttc tccaaaatat gctataataa taccaaaaga
    13741 taaagaagga agacctatga ttaaactact agccttggat atggatggaa ccctcctcaa
    13801 tgaagccaag gaaatcccac aggctcacat cgctgctatt caccaagcca ttgaaaaagg
    13861 tgtcaaactg gttctctgta cgggtcgccc ccttttcggt gtcctcccct actacaaaaa
    13921 actgggactc gacctccaga atgagtatgt gattgttaac aacggttgtt caactcacca
    13981 gactagcgac tggggcttgg ttgactggca agaacttagt ccagctgaca tcgaatacct
    14041 ctatgacctt gctgaaaaga gtgatgttca gttgacactt tttgacgagt cacattattt
    14101 tgttctcggt ggcaagccca atcaagttat tgaaaatgat gctaaactag tattttcaga
    14161 cctgactgaa atttctcttg aagaagcgac tagtggaaag ttccggatgt tccaaggtat
    14221 gtttttagga acaaaagaac aaacagacga ttttgagcag cgttttgctg aagagctttg
    14281 ccaacgattc agtggagttc gttcgcagcc tgtcatttat gaagcaatgc cgcttggaac
    14341 gacaaaggct actgctcttt cacgactagc tgagattttg aagattaatt cctcagagat
    14401 tatggccatg ggcgatgcta ataacgatat cgaaatgctc cagtttgcag ggcttgggat
    14461 tgcaatggga aatgccagcg attatgtcaa atctcttgcg gatgccgtta cctcaagcaa
    14521 cgaagaagac ggcgttgcgc gtgctattga gaaatatatt ctataaaaaa gaaaagcaaa
    14581 tagacagaag ttactgctat ttgctttttt gctaatattt taaaatacgg actaatcaat
    14641 agacaagaat agcgaatcat aaaaatcgat ttactagatg ccatagtatc ttatagttgc
    14701 taataagaag ttagtctagc agaatgaatc tctcatttct cccacactaa tctgtaattt
    14761 gtctatacat ttctggtctt ctatctcgaa acaagcccca gtttagacgt tcacttgctc
    14821 ccttgtctag gtcataagta gctaacagaa cagcttcttc ttgtctttca gctcgttcta
    14881 gaatagctcc tgtttcatcc gtcataaagg aggaaccgta gaagtcaaga ctggaactct
    14941 gtccgccatt ttcctcacta ggagtaacct cctctaaacc ataacgattg gctgcgatga
    15001 ctggaacaat attcgctgct gcgtgccctt gcatagtacg ttgccagtga ccacaactat
    15061 ctgtatccaa aattggctct gaaccgatag ctgtaggata aaagagcaat tcagcaccat
    15121 tcaatgcaag acagcgcgct gtttcaggga accattgatc ccaacagata ccgataccaa
    15181 tcttagcata gcgagtattc cagaccttga aaccagtgtt accaggcgtg aaatagaatt
    15241 tttcttgata ataatggtca tctggtatat gggtctttcg ataaacgccc agcacttccc
    15301 catctgcgtc aatgacggca atagagttat acaagacatt accatctttt tcatagaaac
    15361 tgattggtaa aacaacttgt agttccttag caatcacctt aaaatgctga atggcagtat
    15421 tttccgctac agattgggca tactggtagt agtcatactg acgttcctga cagaaatagg
    15481 gatgttcaaa caactcgggc aagagaataa tttgggctcc ttgctcagca gcctgacgta
    15541 ctaaacgctc tgcggtttgg atatttgttg ccacatcctt agcgcattgc atctgaatgg
    15601 ttgcaactct tacatttctc atctttttct cctattctgg aatttgttgg gtgatacagt
    15661 ggatattgcc accacctaag agaatatctc tggctggtat tccgacaact ttacggtctg
    15721 ggaaacactt gctgaggata tctaaggcca cttggtcgtt tacatcctca aactgtggaa
    15781 ccaagacagc cttgttggcg atataaaagt ttacgtagga agctgctagt cgttcacctg
    15841 cgtatcgctc ttcttctcct tcttcataga tgtagcctgg caaatcttct tctgtcacaa
    15901 cttgtcgaac tgcagggata ggcaatttat gaatggtgaa gtgacaacct tttgcatctg
    15961 tttcctgttc taagagttcg agatctgctt ttgacatggc atactgggga tcattttcgt
    16021 catctgtcca agccaaaaca agctcagcag gaccaacaaa ggcagcaaca ttatcgacgt
    16081 gttcattggt ttcatcctga taaataccat aaggaagcca aataactttt tcagcaccaa
    16141 gactttctaa taatgtgttt tcaatctcct ctttagtcaa gttaggattg cgaccaggac
    16201 taagcaagca actttcagtt acgagaatag ttccttgacc atcgctatgg attgcgcctc
    16261 cttccagtac aaaaggttta gcatcataga caggcctttc taaggcctca gcaaaacgac
    16321 tggctacttg gtcatcctct tcataatctt gataaagacc atcataggtg cctccccaag
    16381 cattgaaggc ccaatccacg gctaatttct tacctttatc atttacgaga atggttgggc
    16441 cagtatcacg cgcccaggca tcattggtgg gaatgtctaa ataaacaacc ttgtctccaa
    16501 gataggattg ggcttcagat agataggcct gctccaccaa aagatagact ctttcccctt
    16561 ctgctatggt ctcgatgatc tgagtaaatg ctcttttagc agcctttcct tgaaaaggcc
    16621 atgatcctgg tcgagtcggc catatcatga gggtaccatg atggggttcg tactctgctg
    16681 gcatgtgata gcctaatttt tttggactgt ccatcatgat aatctccctt taaagtcttg
    16741 atagccaaaa gctttgagta agctacagtc tccctgttcg tccatgagat agagacttgg
    16801 caatccaata ccattaaagg tattattttt gacaaaagaa taaatggcca tgtcttgaaa
    16861 ataaagtctg tctccgattt ggactggatt ttcaaaacta taatcaccaa tcacatcgcc
    16921 cgtcagacag gtattagaag aaagtctgta ggtatgggct ttttcctgtg cctcaaagcc
    16981 atttctcaaa ggtggacgat agggcatctc aagtacatca ggcatatggc aggtcgcaga
    17041 ggcgtctaaa accaagattt ccataccgtt ttctacaata tctaatacct cagttgctaa
    17101 ataacccgca ttaagcgcaa tggcttcacc aggctcgata tagatttcaa gattgtaagt
    17161 ttttcggata cgcttgattt ctgaaatcag caaatccaca tcgtaacctt ctcttgtaat
    17221 atgatgacca ccacccatat tgagccattt tacctcatgt aagtagggac caaactgttc
    17281 ttctacttct ttcaaagttg tttgtaaatc atctgctccc tgctcgcaaa gggtatgaaa
    17341 atgaagtccg tcaaccaaat ctagcaaatc actcggaatc ttgtctatag taactccaaa
    17401 gcgagaacct ggtgcacaag ggtcatagag cgcgtgatct ccttgagttg aacactgagg
    17461 gttgaggcgc aaaccaacac tgacaccagc ctctcgacaa cgcgaaccat gcttacgcaa
    17521 ctgtctctct gagttaaaga cgatatggtc tgttatccca agcaattcct ccatgtcctc
    17581 atccttgaaa gctggtgcaa aaacatggac ttcaccagga aattcttccc ttgccaattt
    17641 ggcctcatag agtccactag ctgtcgtacc tgatagatac tggctaatca agggataagt
    17701 tttgtagagg gaatatgcct tctgggcaag caagaccttg caaccgacct cttcttgtac
    17761 atattgtaga atgcggcaat tagcttctaa cttggccaag tcaataacat aggctggtgt
    17821 tggtacttgt tctaacttca ttagtccacc atttgtggat tttcaaccac aacccatggc
    17881 aaaccatact cattcaaagc ttccatgaat ggatctggat ctaactcctc aaggttatac
    17941 actccagctt gtttccaagt tccgttcatg actaattttg tcccaatcat ggctggaacg
    18001 cctgtcgtgt atgaaattgc ttgtgacccg acttctgcat agcactcttg atggtcgcaa
    18061 acattgtaga tgtagatagt cttttcaacg ccatctttga cacctgtaaa gatacatcca
    18121 atattggttt ttccgactgt acgtgggcca aggctagcag gatctggaag caaggctttc
    18181 aaaaattgaa ttggaacaat ttcttggccg ttaaagttaa tggtatccgt acgaaggagt
    18241 ccaacatttt caagacattt catgtgcgtc aagtaagatt gaccaaaagt cataaagaaa
    18301 cgaatgcgtt tgacacctgg aatgttcttg gccaatgatt cgatttcttc atggtgaagg
    18361 agatacatat ctttttgtcc aacttgaggg aaatcatact cacgcttgat agacatagct
    18421 tcgacttcga cccatttccc atcttcccag taagaacctg gcgcagaaac ctcacggaga
    18481 ttaatttctg gattaaagtt ggttgcaaat ggataaccgt ggtcaccgcc attacagtct
    18541 aaaatgtcga tataatggat ttcatcaaaa tagtgtttga gggcataagc tgaaaagaca
    18601 ctagttacac ctgggtcaaa accagaacca agaagagcag tcaagcctgc ttctttgaat
    18661 ttctcttgat aagcccactg ccatgagtag tcaaagtagg ctgtaaaacc aagttcctta
    18721 caacgttttt cgtagatagc acgccactca gggtcttctg tgtcttctgc ttcgtagttg
    18781 gctgtatcga tatagtgaac acctgttgcc aaacaagcat ccataatggt taaatcttga
    18841 taaggcagag ctacattcaa aacagcttct ggtttgtagc tttcaatcag ggcaatcact
    18901 tcttcaacct tgtcagcatc aagtgctgca gtttcaattt tagtacttgt tttgccttct
    18961 agcttcgctt tcaagtcatc gcattttgac ttggtacggc tagcaatcat aatctctgta
    19021 aatgtttcgc tatcttgaca aatctttgaa atagcaactt gggcaacgcc cccacaacca
    19081 ataactagta aacgactcat ttttttcctt cctcttcttc taaaatgtcc tcaacatact
    19141 tgggcaacat aaaggatccc acgtgtaagt ttgcagtgta gtattctgtg aaaagctggc
    19201 gttttttcca accttccttg tcaaaatctt tgacagggtg gtattttttc gatgcaaatc
    19261 caaacaacca atagccagct gggctagttg gaatatgggc ctgataaacc cgactgattg
    19321 gaaaggcttg attgaccttg cggtgcatgc ttcggcaggc cgactcatcc tcgtcaaaga
    19381 agggactccc atgctggtaa atcatgatgc cgtcttcctt cagagctcga taactattgc
    19441 cgtagaattc cttggtaaag agtccttccg tatggccaaa tggatctgtc gcatcgttga
    19501 tgataatatc gtaatcatct tcgcagtttc gcaaaaagcg tagcccattt tggtagtaaa
    19561 tggtaacacg aggatcatct agccctgcag caaagtctgg gaaatactca cgacagacct
    19621 cgaccaacat ctcatccggt tccacgatat cgatttgttc cagttcagga tagagtgtta
    19681 atacttgggc aacaccgccg tcaccacccc caataaccaa tactttcttt ggatttgggt
    19741 ggacagccat gggaacgtga acggtcattt cattgtagac gaaatcatcc gcatctgaga
    19801 acaagacatg gccatttaaa atcagtattt tcccaaaagc tggcgtatcc aagacttcga
    19861 tatcctgcca ttcacttttt ccagcgtaaa gttgcttggc tgttctcaga gacaatttga
    19921 catctggagt atgaacttca gaaaaccata aatccatcta tttctcctta tattatattt
    19981 agcgcagtta gctactgcca aagcccaagt ggtatacttg gaataagcca ctgtggatta
    20041 gtttattccc tccaatcgct tgacgatttc cgaaaggctc taaccacagt tttttgtttc
    20101 aattggtaat gagtttgata acgcatagac gttttatctc gtctaaggta acaaggcaaa
    20161 aaattcgtgg ataaccaagt agacttgaaa tactacatct ggaggtactc ccatgcttta
    20221 tgtgggcatt gatgtcgcta aaaataaaca cgatgttaca gccttgaatg ttccaggaaa
    20281 aactgttctt aaaccactca ctttttcaaa taataaagct ggttttgaac tcttagatct
    20341 gtctcttcga caactcaacc aagactgtct catcgctctt gaaaatac
//
LOCUS       DAVDWM010000037        20347 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVDWM010000037 DAVDWM010000000
VERSION     DAVDWM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.037"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(112..459)
                     /locus_tag="VUW49_001562"
     CDS             complement(112..459)
                     /locus_tag="VUW49_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7981458.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(570..2345)
                     /gene="spxB"
                     /locus_tag="VUW49_001563"
     CDS             complement(570..2345)
                     /gene="spxB"
                     /locus_tag="VUW49_001563"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET7981459.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2552..4795)
                     /locus_tag="VUW49_001564"
     CDS             complement(2552..4795)
                     /locus_tag="VUW49_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7981460.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QAAGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4805..5176)
                     /locus_tag="VUW49_001565"
     CDS             complement(4805..5176)
                     /locus_tag="VUW49_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7981461.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(5187..5582)
                     /locus_tag="VUW49_001566"
     CDS             complement(5187..5582)
                     /locus_tag="VUW49_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET7981462.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5766..5864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5948..6739
                     /gene="thiD"
                     /locus_tag="VUW49_001567"
     CDS             5948..6739
                     /gene="thiD"
                     /locus_tag="VUW49_001567"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7981463.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKELGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6949..7581)
                     /locus_tag="VUW49_001568"
     CDS             complement(6949..7581)
                     /locus_tag="VUW49_001568"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7981464.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7574..8377)
                     /locus_tag="VUW49_001569"
     CDS             complement(7574..8377)
                     /locus_tag="VUW49_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET7981465.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8377..8901)
                     /gene="thiW"
                     /locus_tag="VUW49_001570"
     CDS             complement(8377..8901)
                     /gene="thiW"
                     /locus_tag="VUW49_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET7981466.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(8906..9598)
                     /gene="tenA"
                     /locus_tag="VUW49_001571"
     CDS             complement(8906..9598)
                     /gene="tenA"
                     /locus_tag="VUW49_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET7981467.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9609..10259)
                     /locus_tag="VUW49_001572"
     CDS             complement(9609..10259)
                     /locus_tag="VUW49_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET7981468.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALTLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10261..11646)
                     /locus_tag="VUW49_001573"
     CDS             complement(10261..11646)
                     /locus_tag="VUW49_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981469.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11647..12207)
                     /locus_tag="VUW49_001574"
     CDS             complement(11647..12207)
                     /locus_tag="VUW49_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7981470.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12469..12557)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12766..13395)
                     /locus_tag="VUW49_001575"
     CDS             complement(12766..13395)
                     /locus_tag="VUW49_001575"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7981471.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13397..14179)
                     /gene="thiM"
                     /locus_tag="VUW49_001576"
     CDS             complement(13397..14179)
                     /gene="thiM"
                     /locus_tag="VUW49_001576"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET7981472.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14461..15129)
                     /locus_tag="VUW49_001577"
     CDS             complement(14461..15129)
                     /locus_tag="VUW49_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET7981473.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15344..15432)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15686..16822)
                     /gene="lctO"
                     /locus_tag="VUW49_001578"
     CDS             complement(15686..16822)
                     /gene="lctO"
                     /locus_tag="VUW49_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET7981474.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTLHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17446..18936)
                     /gene="lysS"
                     /locus_tag="VUW49_001579"
     CDS             complement(17446..18936)
                     /gene="lysS"
                     /locus_tag="VUW49_001579"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7981475.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19064..19210)
                     /locus_tag="VUW49_001580"
     CDS             complement(19064..19210)
                     /locus_tag="VUW49_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET7981476.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            19243..19863
                     /locus_tag="VUW49_001581"
     CDS             19243..19863
                     /locus_tag="VUW49_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7981477.1"
                     /translation="MYIAVWGSLGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFRSGLEAISQTQQEIGLA
                     IGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSVFSAVALADLMYVAKDLIGLY
                     YETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPSTLSRK"
     gene            19823..>20347
                     /locus_tag="VUW49_001582"
     CDS             19823..>20347
                     /locus_tag="VUW49_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7981478.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINTSGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEV"
BASE COUNT         6008 a         4489 c         3791 g         6059 t
ORIGIN      
        1 atatgcaatt tctttatata aaaagaccgg attgctccga tctttcaata gttcatattc
       61 tcaatttcta ttttaaaaat agctaaggtt aacgtcaaat gactacgcga cctatttcat
      121 acgataaaaa tcaagcacta gaccagcagg tccttgaact aataaggact ctgttcccca
      181 atcggttaca gttggtccgt gtaaaacctt tataccaagc tcgttcaacc gtttgtagtt
      241 ctggtctaca tcctcaacct cgatatgaat aatgattcct gactgaaagt tttccaaagg
      301 aaccaaatga ttttgtgaca acataaggca gtgactacca atcgtaaact gagcaaaacc
      361 atcatcagca taatctgcct ttttatccaa gatatgctcc aagtcagcac agacttgggg
      421 aacatttgaa acgataatat ctaattgatt taaattcatt tactctcctc cataaaaaga
      481 ccggattgct ccgatctttt aaagttctgc tctatgaaaa tcaaagaata aagtctacaa
      541 gtttcatatt tgattttcgg cgagaggaat tatttaattg cgcgtgattg caatccttct
      601 tcttccaaga agagacggaa tggtacgagt tcttctgctt cgtatttttc cttgaaggct
      661 ttgatagctt cttctgagtg aagttttgga tccaattcaa gtacttctac tggaagtgga
      721 cggtgttgag tgatgcgagc atcgatgaca acagttttac cttctttgtt caatttaaca
      781 gcttctgcaa caactgcatc gatgtcttcg atacggtcaa ctgtgaatcc aacagctcct
      841 tgagcttccg caattttagc gtagtcagcg tttgtgaagt ctacaccaaa caagtgtttg
      901 tttgtatctt cgtatttgtt cttgatgaag ccgtactcag catttgagaa gacaaggttg
      961 ataactggaa ggtcgtattg aacgtttgtg ataacgtctg ggtagcacat gttgaatgct
     1021 ccgtcaccca tgatgttcca tacttggcga tctggattgt ctttcttagc agcgatacca
     1081 ccaggaaggg caatacccat tgtcgcaaag agtggagatg tacgccacat gttcttaggt
     1141 gtcatgtgaa ggtgacgagt agatgtttga gtagtgttac ctacgtcgat tgagtagata
     1201 gcgtcttgat cagcatgttt gttgattgca ttgtaaactt gatacaattg caattcaccc
     1261 tcagttttac cttcgagttt gttcatgtaa tcacgccagt tttggttgtt cttaacgttt
     1321 gcacgccacc atggagttga ttcaactggg tttactttgt caaggatagc tttagctgct
     1381 tgaccagcat caccaaggat tgaagcgtca agggcatgac gtttaccaag tttgtaaggg
     1441 tcgatatcga cttggatgaa tttttcagtg ttcttgaatg cttcgtaaac ttcagcaaat
     1501 gggaagtttg aaccaaggaa aagaactgtg tctgcttcaa agaccacttc gttggctggt
     1561 ttccaaccaa cacggtaagc agaacctgtc aaaccttcat agttccattc gaaagcttca
     1621 aagtttttac cagttgtgat gattggtgct ttgattttac gtgacaattc agtaatcact
     1681 tcaccagctt taacaccacc aaatccagca tagataactg ggcgttcagc attgttcaag
     1741 atttcaacag ctttgtcgat ttcaacttcg ttcaaagcag gagcgatgaa tgagcgttcg
     1801 tatgaacctg aaccgtagta tgagttttca tcgatttctt ggaaaccgaa gtttactgga
     1861 atttcaacaa cagctggacc ttttttagaa actgcagcac ggcaggcttc gtcaattact
     1921 tttggcaatt gctcagcgta agctacacgt ttgttgtaaa cagcgatacc gttgtacatt
     1981 gggttttgat taagctcttg gaaagcatcc atgttcaatt cgttaactgg acgtgatcca
     2041 aggatcgcta ggaatggagt gttatccata gctgcatcgt aaacaccgtt aatcaagtga
     2101 gtcgcacctg gaccacctga accaactgca accccgattg agccgccgaa tttagcttgc
     2161 ataaccgctg caagagcacc tgtctcttcg tggcgaactt gtaagaaacg gatatctttg
     2221 tcttcagcca aagcatccat caatgagctg agtgttcctg atgggatacc gtagattgta
     2281 tctacgcccc atgttttcaa tacgttaagc attgctgcag atgcagtaat tttcccttga
     2341 gtcataatga taactctcct tcaatttttt taaacttgga gaatacgatt acatagaatt
     2401 ggaaacgttc tccaaatttt tactattcca ctgtatcata tttatgctga cttttctaaa
     2461 aatctgctca aaactctcta ttctctattc taatacagtt ttgaaagtgt tgtcatttct
     2521 gttttataac aaagaaatct agtcattact tttagtctat tttactaaaa tttaacagaa
     2581 gggaactggt cagaacagat acagaactaa aggccatggc tagacctgcc aattctgggt
     2641 tgagagccag tccaacacct gaaaagactc ctgctgcaat cggaattccg acaacattgt
     2701 agataaaagc ccagaaaaga ttgagtagaa ttcgatgaaa ggttttctta ctcatatcaa
     2761 aggcacgaac cactcctaaa agattattgg ttgtcaacac caaatctgct gactcgatgg
     2821 cgatatctgt tccagcaccc atagcaattc ccacatctgc tacactaagg gcaggagcgt
     2881 cattgatacc gtccccaaca aaggctactt tgccagccgc ttgcagttta tggatttcat
     2941 gggctttttc ttctggcaag acgcctgcaa tgacctcttc aattccgatt tgatctgcaa
     3001 tagcacgcgc cacaccagca ttgtctcctg tcagcatgac tgttcggaga ccacgttttt
     3061 ttagctgact gatggctagc ttagcatttt ccttaggaat atcttgcaaa gcaagcaagc
     3121 ctttgatttc attgtcaaca gctaaaaaca caactgtctt agcttctttt tctagttctt
     3181 ctagtttatc ttgataagta ttagaaatat ccatgccatc cagcatttta gcatttccaa
     3241 gtaaaacttg ttttccattg attcgccctg aaacaccttt cccgtgcaag gcctgaaaat
     3301 tttcaacagt ttgaaactca agtccagctt cactcgctcg cttaacgata gcctcagcca
     3361 gtgggtgttg agaagcatct tccaaggagg ctgccaatcc aaacacttct acttcgtcgc
     3421 cgatgatatc tgttaccaca ggtttccctt ccgtcaaagt cccggtctta tcaaagacaa
     3481 gagtttgaac tttctggatt tcctgtaaga cagttccatt tttgaggaga acccccatct
     3541 tggcactacg tcctgtcccc accataaggg ctgtcggtgt tgcaagtccc aaggcacaag
     3601 gacaggcgat aatcaaaacc gccactccgt agagaagaga ggacacaaag ctagctccaa
     3661 gcacgaccac actatccctg agcaagacga accaaaccca aaaggtcatg attcctaaaa
     3721 tgacaactac tgggacaaaa atccctgaaa tcttatccgt caagtcctga atcggcgcac
     3781 gacttgtctg agctttcttc acaaaatcca caatctgagc caaaacagtc tctgagccaa
     3841 ctttttctgc tctaaagaca agcgttccac tatgattgat ggttgagcca atgacagtat
     3901 ctccaactgt cttgtccaca ggcagactct cacctgtcac catggattcg tcaatactag
     3961 agacaccttc tactacgaca ccatcaacag caatcttttc accgggacgc actcgaatca
     4021 ggtcgcctac cttgacttgt tccaaaggaa cttggacata actatcatca ctcaagactt
     4081 ctgcggtttt agcttgcaag tccagtaatt tctccacagc ttgggacgta ttttttctca
     4141 ttttttcctc aaaaactgct cccaaaagaa cgaaaaagag gataaatcca gcactttcga
     4201 agtaaacagg gagaccagca aagagagcaa ctaggctata gaaataagcc actagagttc
     4261 ccagcgcaac caaggtatcc atgttggcat tgtgcttttt aaaactggcc caagcactct
     4321 ggatatatgg cttacctgca actaacataa taggcgttgt tgctagaaag gttccccaat
     4381 gcatgacttg atgactaatg ctacctgcca acatcccaat catgagaatc acaagaggca
     4441 cagtacagat actagtaatc caaaaacgtt gcaggagaga tagagatttt cgagtcttct
     4501 caacgactgt atagcttccc ttttgcatct tcatgccaca agaaaattca tgtcgcccta
     4561 attcttgagg tgtaaaacga atgactttct cctcatctac gccgattggt tccaagatac
     4621 cttcttcttc aaacagaatt tccttataac agtttgaagg agtagcacga tgaaaggtaa
     4681 tctcagctgg aattcccttt tgaagctgga tatgggctgg atgatagcct ttttcagctt
     4741 ggatacggat tttttgaatg ccattttcta agcttgcttt cacaatttct gtcatagtct
     4801 ccacctactc tacaatcatc ttgccgtgca tcatgttcat accacaagca aagctaaact
     4861 ctccagcctg ttcaggcgtg atttccacta catactcttc ccccattggc aggttcgcat
     4921 gtacaccaaa atctggaaaa acaatttgat ccagacatgg tgaaggatcc ttgcggtcaa
     4981 agacaatgcg ggctggcact gatttcttga ggacaatcaa ctcaggagta tagcctccca
     5041 tgacttccac tcgaatctct tggtatccgt ttttttgctg ggctttttgt ccagattttt
     5101 caggcttttt gaaaaaccaa aacaagataa acgcgataag ggcaatacaa ataatggtta
     5161 caatactatt taacatgacg tctcctttac atacaattac atcttacttc tgttacagca
     5221 cttgatttct tctctgaaat cacagcttcc aagtcttcca agtcagtctg agtaaattca
     5281 cattctacaa tcaagtcagc caacaaattc ctaatcctac gggaacaaac cttgtctttg
     5341 atatcttgga caagtaaatc ccgactttgg tctagagtta aaagcgctga ataaacaaag
     5401 gacttgcctt cttttttccg agtcaaacac tctttctcaa ctagacgagc caaaagtgtc
     5461 tgaaccgtgg acttggacca gtcaaaccgc tctgccaaaa ccctaatcaa atctgtactg
     5521 gtctgctccc cctgcatcca aataatcttc atgacctgcc attctgcatc tgaaatctgc
     5581 attaccatac ctccaaaatc tacatttgtc aattacactc aacagtatac tcccaaaatc
     5641 tacatttgtc aattatagaa ataatatttt cttcgaaaaa tagaatttta atcatttgaa
     5701 aaacgatttg cagtcaaata ttactatata aacaataaaa atatgctata ctaaagaaaa
     5761 aagaaaacaa ccactagggg tgcgtaaagc tgagattaac gactgttaga tccctctgac
     5821 tcaatctagg taatgctagc tgatggaagt ggaaatgata atggggacta gcagtcttct
     5881 attgcctttc taaaacagac tagcttgttc ttaagaatac aaacttcagt tggttgggag
     5941 gttttagatg acttatttac ccgttgcttt gaccattgca gggactgacc ctagtggtgg
     6001 tgctggcatt atggcagatt taaagtcatt ccaagcgaga gatgtctatg gaatggctgt
     6061 tgtaaccagt cttgtcgctc aaaataccag aggtgttcag ctaatcgagc acgtttctcc
     6121 tcaaatgttg aaagcccaat tggagagtgt cttttctgat attccacctc aggctgtaaa
     6181 aactggaatg ttggctacta ctgaaatcat ggaaatcatc caaccctatc ttaaaaaact
     6241 ggattgtccc tatgtccttg atcctgttat ggttgctaca agtggagatg ccttgattga
     6301 ctcaagtgct agagactatc tcaaaacaaa cttactacct ctagcaacta ttattacgcc
     6361 aaatcttcct gaagcagaag agattgttgg tttttcaatc catgaccccg aagacatgca
     6421 gcgtgctggt cgcctgattt taaaagaatt gggtcctcag tctgtggtta tcaaaggcgg
     6481 acatctcaaa ggtggtgcta aagatttcct ctttaccaag aatgaacaat ttgtctggga
     6541 aagcccacga attcaaacct gtcacaccca tggtactgga tgtacctttg ctgcagtgat
     6601 tactgctgaa ctagccaagg gcaagagtct ttaccaggca gttgataagg ccaaggcctt
     6661 tatcacaaaa gctattcaag atgcccctca actcggtcat ggttctggtc cagtcaacca
     6721 tacaactttt aaagattaag aaaaaaaact ctctagttcc cactttaagg gaattagaga
     6781 gtttttatac tcttcgaaaa tctcttcaaa ctacgtcagc ttccatctgc aacttcaaaa
     6841 cactgttttg agctgacttc gtcagtctta tctaaaacct caaggcagta ctttgagcaa
     6901 cctgcgacta gctttctagt ttactctttg attttcattg agtattaatt aggaaagaat
     6961 gtcatgcaac tttgttaaaa aggcttgcgt ttttgcctca atatcttctg cttgcatcaa
     7021 atcacgtaca acagctacac cagctatgcc agtgcccata agctgatcaa tattctccga
     7081 agtcaagcca ccaatagcaa ctattggaat ggcaaccgtt tggcaaattg ttttcaaggt
     7141 tgaaatcagg gtgatgggtg cattttcctt ggtggttgtc ggaaaaatgg ctcctgtccc
     7201 caagtaatct gcacctgatt tctccgcttc cagagctctt ttaaccgttt tagcggtgac
     7261 accgaggatt ttttcaggac ccaagacttg tcgggcaacc gaaactggga gttcgtcatc
     7321 tccaatatgc agaccagcag catcaaccgc aagacaaaca tccaaccgat catcgattat
     7381 caagggtacc tgataagcat ctgttatttc cttgacttgt tttgccagtt gataatattg
     7441 attggttgtg agattttttt ctcgcaattg gactatggta acccctgaac ggcaggccgt
     7501 ctcaactttt gcaagaaagc tttccacgga atcttgatag cgattggtta ccagatatag
     7561 tctaagtgct tctctattca taaacctctc ctttgatggt atctagccaa ttttcatctc
     7621 ttcttaggag cgaaagctga ttgagtactt ggtaacgaaa ttcttccaat cccattcctt
     7681 gaacaactat tttctcagca gcgatattga gataagagac tgctaagcaa gaagcttcaa
     7741 aaccagtctt tccttggctg agaaaaacgg ctgttaaggc tccaaccaaa tctcctgtcc
     7801 ctgttatcca gtctaattct gcacagccat ttcccagtac agcgacctga tttttcgaaa
     7861 cgacgaggtc cttgggacct gtgactaaga aagacatacc atgataggtc tgacaccagt
     7921 ctttcaagac ttgaagcaaa tcctccgttt cttgatcttt agcactcgca tcgaccccaa
     7981 cgccgtggtg ctttaatcca acaagacttc gaatttctga catgtttcct ttaaggaccg
     8041 taggtctata gtctaaaagg tctctgacta agctcttacg aatggatgaa gctgttacgc
     8101 caaccgcatc tactaccatt gggagacaaa cttgagctgc ataagaagct gctatacgga
     8161 ttgctttctc cttctcagct gacaaatgcc ccaaattgat gaagagagcc tgactttgct
     8221 tagtaaaatc aagaacctca cggggatcat ctgccatgac aggtttgcat cccagagcca
     8281 aaatcccatt tgccagcatc tcacaagaaa tctcattggt catacagtga atgagggaac
     8341 tagagcctat aggaaaggga tttgtaaatt cctgcatcag tctatccttt cactaaagaa
     8401 atatccctgc acttttttaa agaattcctg cttgattaaa aatcgaaagg caataaagga
     8461 aatcgctgta ccaatcaagg ttgctccgaa aaatcgaggc gtgtagataa accagctaag
     8521 cttagcagct gatcctgtaa agagtaccat aacaggatag gaaacaatag aaccaataat
     8581 acctgttccc acaatttctc ccaaggcaga aaagtaaaat tttcgaccgt acttataaaa
     8641 gagacctgct agaagggctc caaaagtcgc tcctgtgaga gctaaaggcg gaatcccttg
     8701 agtcgtcata cggataaagg ctgtcactgt agccatagcc aaggcataaa caggtcccat
     8761 catgattccc gctagaatat tgactacact ggacatcggt gccattccct caatccgaaa
     8821 gataggtgta aggactacat caagggcaat catcatagat aaaatggtca atttgtgaac
     8881 ttgtagttgg tgctttctca tgtttctatt cttctccttt ttctaaagac tgtaaatcgc
     8941 tcttccatgt ctggtgttgg taagccatct cccaaaactt ggcttccata tgaacactga
     9001 tgtggaaggc atctagcatt ttttgcttat ctgtctcatc actttctcga tagagctgat
     9061 tgaccagtgc tccctcctct ctgatctgtt gctctaactc atccgtaata taagtttcaa
     9121 tccattgttg atagagagga tttggtgatg gtttaagatt aagtgatttg cctatatcat
     9181 ggtataacca aggacaagga agcaagcttg caaaagcgat ggctaagttc ggttctgcaa
     9241 attgcctata aatatgagaa atgtaatgat aacaggttgg agcgattgga tgttgctcca
     9301 tttcctggtc gctgatttcc aattccttga aaaattgttg gcgaataaat aactcaccct
     9361 ccactaaacc ctgagcattt tgtttcaaga gtcttttcat ctcttggttt gaagtcttat
     9421 cagccaaaag atgatagatt tctgagaaag ccttcagata gtaggcatcc tgaatcaggt
     9481 aatagcggaa aatggcaggt tctaaattcc cctcttgtaa ttgtaaaata aagggatgat
     9541 gaaaggaagc ctgccaagct ttcttggata attccatcgc aatatctgta aattccataa
     9601 taactccttt ataaaaatag actggtttga agcaataaaa agaaaagcag gtagattaat
     9661 tttgtttttt taggaatata aaaagtccga tagctattct tcaactgtgc atgttcgtca
     9721 tatccgtgag cagatagagc tctcaggtaa agatggcgcc acctaaagac tgtcatcaga
     9781 accttactgt aaatcaaggg cgaccaaaaa tgtagttctt gaccacgtaa taggcaagct
     9841 tctttgaggg acttgatttc ttgctgaatg agaggaaaag aattgaatac cacaatcaag
     9901 gcataggacc aagagcgtga tagccccttt tgagccaagt acaagagaag ctcttttagt
     9961 gaaatagagg aaacaaagac aaggccgata caaactgtca caaaggccct cgttccaagc
    10021 atgactgcct gtgaagcatc tccgtgtaac tgaactgccc agtagttggc aaaagatggt
    10081 aaaatggcaa gtatgatcat ccaagctaac attttaaatc gacggtaata gagcataaag
    10141 agaatacaaa atgcgactac cgaaagagtc agagcaatcg aaggaatgaa agatgtttcc
    10201 aaggataaaa tcagcaagaa gagactgata atcggtgtct gggttgctac tttgaccata
    10261 ctatctcacc tccccttggg tattgctact ctgagatgta agtggtttgg taatggtcac
    10321 ttctttcaca tgccgaagac cctgactagt catctcaatc caataatcaa ccacagaaat
    10381 caaagggtct aaacgatgac taatgagcag aaaacttctt ccttgattcc tctcctccac
    10441 aatccacttg caaaaataat ggcaggctct atcatccaaa cctgcaaaag gttcatctag
    10501 caagatcacg gaagccttac tggtcaagat ggtcaagagc tgaagaattt tttgctgacc
    10561 accacttaat tgatagggac tcttatcgac tgcctgctcc aaatcaaaat atcgtaaagc
    10621 ttgaaaaatc cgctgatttc tttcagaatc aggtccatct aattgaagct cctctcgcag
    10681 actgactcgg ataaactgct tctcagcttc ctgaacaaca ccagtcagat cacgatacaa
    10741 actctttttc tttttcagga ccgaaccctt ccaagtaatg ctccccttat acttttgaaa
    10801 ttgaagaata gaccgaaaga gggttgattt cccgacacca ttgtcaccca ggatacagga
    10861 aatcccttga tagaatgtga aatcagcaat tgaaaagagg gggcgattac caagctcacc
    10921 agtcacacgg ttcatatgga atagttccgg gctagaagca acttcctttg aagcaacctg
    10981 tgtcatctca taggaaggga tttgaaacac ttcccttagt tttccgtctc ttagctccac
    11041 catatggtcg atataggctt tatagtcaga taaatcatgg tcgcacaaaa taactgtctt
    11101 cccatcaaga gccaactctt ttagaatctc caatatctcg attctactct tgcggtcaat
    11161 ggaagcgaag ggctcatcca agagatagac cctaggattc atggcaaaga ggacagccag
    11221 cgctgctttt tgcttttccc cacctgatag gtgatgaatg ggacggtgta agattgcctc
    11281 gcaacgacat tgctggacaa cctctgctat tttagaatca atttcctgaa ggtgatagcc
    11341 gatattctcc aaggtgaaaa tcaactcctc aaacaagttc tccatggtaa attgatgatt
    11401 gggattttgc aagagaatgc caaccttctg gacacgtgcg atgatagaaa gctgactgac
    11461 ctcgctccca tctatcagga cttgaccact atagggaaga gaactgactt gggcaatgac
    11521 ttgaaaaagg ctggattttc ctgaaccact gctcccaact aacaaggtaa aggcttgcgc
    11581 atgaaaagta aaatcaaacg gctcagagaa gattggggac tgaatcgctc gtagttccag
    11641 acccatctat gcttttcctc cagttgcaaa ctgatgatag agtttgacaa tggcacgaac
    11701 caagatggta cagaagaaat aaacagaaat aaaacgtacc acaagtaagg aaaggacaaa
    11761 cggaagggaa aaggcgtagt aacctaactt aatgtattca tagacaaagc taacaagcgt
    11821 aatcccaata ctattagcag ttagagagag ccaactttca tagcgattct tagttacgat
    11881 aaaaccaaat tcacttccca aaccttgaac taagccagac aaaagagctc ctagaccaaa
    11941 ttggctacca taaaggactt cagcaagcgc agctagcact tctccaatcg ttgcacttcc
    12001 gactctcgga acaaagatgg cagcaatggg cgcagccata caccagagac cgaagaggat
    12061 ttcattggca aaggcctgca aaccaagagg tgttaagagt agactgagaa tattatacac
    12121 atatcctgaa ccaacgaaaa ccccaccaaa aaagatagac aagaaagcaa gcaagataac
    12181 atcttttaac tgccattttt tcaacataaa aaactccttt tttaaaagaa aagtggggca
    12241 ctcaagaaga ccgacctaaa tactttgtat agcagattga atttagaaca gtacacagga
    12301 atgctaaaat attcctagaa attaatttga attttctaat tgatttgttc gcatcttatt
    12361 tcaatctact atatcatctt catccagttt cgtaaaagaa aaaactctaa ttacagatac
    12421 gaattagagt tcagcttaca agattagaca gttcttttcg acatacgaaa aaaacatttc
    12481 acatttccct tcgccagtct taactgtatc aggttcaatg ggtatcatct cagcctaaag
    12541 caccccaaat gcctttatta tttaattatg tgattattat aacacacatt ttatactagt
    12601 tcaagaaatt gaactggaaa tacagccttg cactcacaaa gacagcagat ctttcttttg
    12661 caaaaaacaa atgacctgtt tgatgaatta gccattcaag ctgaatctag acatagtttt
    12721 ttaaaaaagg aaaatcctac ttacttagaa tccaaggata gatatctatt gttcactcat
    12781 ttcccgaaca gttttttcta tattttttgc atacgatatt gccgaaatga ttgaaacgcc
    12841 atccatattg gtctttataa tgtctttaat atgtttcgtc tgtatcccac caattgcaac
    12901 taaaggcatt tgtggcaata gttttctcat caattcaaga ccttcataac ctatagcacc
    12961 accagcatca tcttttgact gggtatcaaa tacaggccca acacctacat aatctacata
    13021 ttcaactttt gattgttgaa attcttcttc gtttcttata gaaagaccaa ttattttatc
    13081 tggcatcaat tttctaattt catcaacacc aatatcatct tgacctacat gtacgccatc
    13141 ggcgtcaatt tccattgcta aatctatatc gtcattaacg ataaatggaa cattgtattt
    13201 tttacaaagt tctttaattt ggatagctag ctcaagtttt tctaagcctt ctaaagcacc
    13261 ctcacctttt tctcgaaatt gaaataaggt tataccacct tttaaggctt cctcaacgac
    13321 tgtatataga ttttttcctt ggcaagtagt cgttccacaa ataaaatata gttttagtaa
    13381 ttctttatga aacatcttac ttcactcttt tgaattcctt tacatcttca tctgtaatct
    13441 cgtataaggc atttataaat tcaactttaa atgtcccagg aagatgtcca tttggacgtt
    13501 tttctgccat ttctccagcg atattgtaaa ctaacattgc tgtttctaat gatttcaatt
    13561 cttgaccttt ttctagtccg ataaagcttg ctactacagc tcctaataag catcctgtcc
    13621 caatgacttt cggcatcata gcactaccat tatgaatcgt taccacttct ccattaacag
    13681 caatggcatc cacttcacct gttactacta ttggaatatt gaacttctca tttgctgcta
    13741 gagcaatctc gtcaatatta tctactcccg cactatctac tcctttagat gccacatcta
    13801 ttcctactaa agaggcaatc tctccagcat tccccctaat cgcagctagt ttgtaattgt
    13861 tgattaaatc atctgctact ttttttctat attctcctgc tccacaggct acaggatcta
    13921 aaactgctgg gacattatat ttctctgcaa ttttcagagc agcttggtat aatttccaat
    13981 tttcatctgt caatgttcct atgtttatta ataaaccacc agcatacttt aataagtcct
    14041 ctaaatctgc tggaaactca ctcatggctg gtgaggcgcc cagtgctact aatccatttg
    14101 ctgtgaaatt ttttactaca tcattggtga tgcaaatgac caaaggtgct ttttctttta
    14161 ataattttaa acttgtcata ttgaaatcct tccttttcac tttatacgat ctactaattt
    14221 cgatttatct ttagttgaga atttttttca tttacattga atgatttata ctcaatgaaa
    14281 atcaaagagc aaactaggag gctaaccgca ggttgctcaa aacactgctt tgagcctgta
    14341 gataagactg acgaagtcag ctcaaaacac tgttttgagg ttgtggatag aactgacgtg
    14401 gtttgaagag attttcgaag agtcttacct catcaaattt gtaaatatca tgagccttct
    14461 ctagacatcg taaccaatat caaaaaaagc taattctaaa gcgactgctt gattccagcg
    14521 ttgctgaagt tctgtcaaat cttctcgatt tttaccgaca cgattgagtt cgtcaaccag
    14581 aaattgaacc cactctgcaa agaaaggacc tctgtggaga ttgatccatt ccgaatgaat
    14641 atagacttca ggtagagcca aatctttaga accccagtct aaatagagac cttctgcaat
    14701 gaccagcata accaaaagat gggcatagtc tgatgaagcc actgccgaat acattagatc
    14761 ctgaaaggct tttgttacag ggtgcaaagt cacttctaga tagtcattct ctgctacttt
    14821 taactcttta aaagcctttt ggaaataacc atcttcatct gcttcaagaa agcctagttg
    14881 cttggcaaaa cgaagcttgg attcaagttt atctgcgtga gctacgcagg cacccagcat
    14941 ggataagaag gcatcaaaga agtgataatc ttgaatcaga tagtccttta agaccttatt
    15001 ctcaattgtc cccgcaaaaa gttccttaac aaaacgatga ttgattgcag cctgccaatc
    15061 cttctgactg ctttttaata attctccaac agtcaaacct ggctgaaatg catagtcttg
    15121 tgtttccata tttacttctc ctctctttac ttgttagtaa ttaataaaac accaagaaat
    15181 atcaagcaaa atcgtaattc cacttgatcc ttttaaagca catcgagagc atttgcagag
    15241 agctaactaa acaagcctat ccagtttata taaacaaaaa actccaatta caatcaagaa
    15301 ttagagttga cttacaagat tagaccgttc atttcgccat acgaaaaaac gtttcacatt
    15361 tcccttcgcc agtcttaact gtatcaggtt caatgggtat tatctcagcc taaagcaccc
    15421 caaatgtctc tattatttaa ttactgaacc agtatagcaa aaaatgaaag ccctagcaag
    15481 atatttgacc gaaaaatatc tttatatata atatattgaa actagaatag tacacctctg
    15541 cttctaaaac attgttagaa atcgatttaa ctgtcctgat taatttgtcc tattcttatt
    15601 tcattttatt atagttttcg atagcgattt attcttccaa tacacgaaga aaaacctcca
    15661 cattcagtgg aggcaatctg ttttatcaat acaattttaa gtcacgaggg tcaactggga
    15721 aggttgggtt gtatggattg tgacggagct taaagtgttt gacatcttca atggtctgag
    15781 ctccagacaa ttgcataact gtcttcaatt ccgcattcaa gtgttcaaag acttgacgca
    15841 caccgacact accaccgaga gccaagccat aaataacagg gcgaccaata gctaccaagt
    15901 ctgctcctga agccaaggct ttaaagacgt gttgaccacg acgaacacca gagtcaaaga
    15961 caatcggcac acgtctatca actgcttctg ccacttcttg aagcgagtca aaggcagctg
    16021 gtccaccgtc gatttgacga ccaccgtggt tggttaccca gataccagaa gctcctgcag
    16081 caagtgaacg ttcaacgtcc tcacggcatt gtggtccctt gacatacaca ggaagaccag
    16141 agtattcagc gataaattct acatcgcgtg gagacaagcg ttgtttagct gatttgtaaa
    16201 caaagtccat tgatttacca gcaccttctg gcaggtattc ttcaacaatc ggcatgccaa
    16261 ctgggaagac aaaaccatta cgcttatcca cttcacgatt cccccctaca gtagcatctg
    16321 ccgtcaagac aatcgcttta taaccttcag ccttcacacg gtccatgatg tggcggttga
    16381 taccgtcatc cttactaaag taaaattgaa accaatgaag tgtcccttga agggcttcag
    16441 aaatctctgg aaggtcaaca gtagagtaag aactggttgt ataaagagaa ccaaactcat
    16501 gcacaccacg cgcagtcgcc acttccccct gttcatttgc caatttatga gccgcaacag
    16561 gtgccataat gattggtgaa gatagttttt cacctgcaaa ttcaatctct gtacttggat
    16621 tttctacatt gcaaagtgta tgaggaacga tgagtttgtg gttaaaggcg cggatattct
    16681 ctcttaaagt gaaagtatct tccgccccac tagcgatata gccaaatgct gctttaggaa
    16741 taacttgttg cgccattggc tccaaatcat aggtattgat gaagtctaca tgaccttctg
    16801 cattgcttgt tttgtatgac ataaaatgcc ctccttgatt aagtaagcgt ttgcttttta
    16861 ctacacaact ataataactc ttttttcaat cattttcaag agttttgctt aaaaaccatc
    16921 ttattttttc atacaatata ctagccctac tttccttaaa ctctaaactt tagtctatat
    16981 taagtgaatc gttgagattt tttgcttcca ggcatttgaa ctactttttt atttgctgat
    17041 tctatctact aatactataa aaaacaagtg aataagacac cagaacatcc tctaatagga
    17101 cctatggact tatatcagtc ccatatatac tgtttaagca agatactatc ctagttgacg
    17161 ctaacttcct atcccactta ttaatttttt atttatagtg aatctgaaca aatcaattgg
    17221 ggaatccaag tcaatttcta gtactttttg aaaagtttca gcgtactatc gtaaaatgaa
    17281 ataaaacatg cacaaattgc ttagggaatt taaaccaatt tctaacaatg tcttagaaat
    17341 caaagtgtac tatttcaact tcaatatact acacttcatt ttctccttaa attcagtctc
    17401 tttttgaatc aaaaaatcct ctggtaagag ccagaggata agaatttatt tcattgttgg
    17461 gaagagcaat acatcacgga tagttgttgt atcagtgagg agcatgcaga gacggtcgat
    17521 accgattccc aaaccacctg ttggtggcat accgtattca agagcttcaa tgtagtcata
    17581 gtcgattcct gtcgcttcat catcaccaag ttctttggct ttagcttggg cttcaaaacg
    17641 gctaagttgg tcgattgggt cgttgagttc agtaaaggca ttaccgtact ccttagtcat
    17701 gataaagagc tcgaaacggt cagtaaagcg ttggtcttca ggatttttct tagcgagtgg
    17761 agatacagct actggatgtc catagacaaa ggttggttgg attaaagttt cttcaacaaa
    17821 ctcttcaaag aaggcattga tgatgtgacc aacctcagtg tagtgtttct caactggaac
    17881 tttcttctca gcagcgatag ctttagcttc ttctaaagtc atgtcttgcc agaaatcgac
    17941 accagtaatt tctctgatag catccaccat atgaacacgc ttaaatggtt cgttaatctt
    18001 gatttcagta ccttggtagt tgactgggcc atcgccctta actgatttag cagcgtgttg
    18061 gataatgcct tccgtcaagt ccatgatgtc ttggaagtct gcataagctt ggtaaacttc
    18121 gatagaagta aactcagggt tatgagtagc gtccattcct tcgttacgga agatacggcc
    18181 aatttcatag acacgttcca taccacccac gataaggcgt tttaagtgaa gttcagtcgc
    18241 gatacgaagc accatgtcaa tgttttgggc attgtggtgg gtgataaatg gacgggcagc
    18301 agcaccacca gcttcgttgt gaagaacagg tgtttccact tcaaggaatc ctttttggtc
    18361 aaggtaacga cggatttcag agatgatttt tgaacgagtg acaaaacgtt caaagctttc
    18421 acgattagaa atcaagtcaa ggtaacgttt acggtaaatt gtttcaacgt ctgtcaaacc
    18481 atggaatttc tcaggaagag gacgaagagc cttagacaag tgtgtgatgt gggttgcctt
    18541 gatagagagt tctcccatat ccgtacgcat cacttcacct tcgacaccaa ggaagtcacc
    18601 aagatctgct tttttgaaga tttcgtagtt ttcttcaccg acagcatcct tacgaacgta
    18661 gatctgaatc tggccttcgc ggtcttgaag gtgggcaaaa ccaacttttc ctttaccacg
    18721 tttggttatc aagcgtcctg cgatagtagc tgtttcgttt ttatcgtgta attgttcttt
    18781 atcgaggttg gcatatttat cttttaattc ttgtgaattt gcagtacgtt caaaacgttt
    18841 tccgaaagga tcgattcctt gttcgcggag cgcagccatt ttttcacggc gaacgatctg
    18901 ctggtcattt agttcttcca tatgttctgt agacatggga tcctcctagt tttactcaaa
    18961 attgaatacg atgagtcatt ttttgcgata ctcccatttt atcataaata agcaagaaat
    19021 tcacggataa tctttaaaaa ctaaaaagaa cctgacgcta aaatcactgg aaagaagtga
    19081 aagtatctcc cgccccacta gcgatatagc caaatgctgc tttaggaata acttgttgcg
    19141 ccattggctc caaatcatag gtattgatga aatctacatg accttctgca ttgcttgttt
    19201 tgtatgacat aaaatgccct ccttgataag taagcgttta ctttgtatat tgcagtttgg
    19261 ggaagtttgg gatcctttct gctcggttta atcgttagta tcatccgaca ttatcgaatc
    19321 cctgttttgg cgcaagtagc gacagcctac attgaattgt cacgtaatac gccccttttg
    19381 attcaactct tctttctcta cttcggtctt ccccgaatcg ggattgtcct atcttcagaa
    19441 gtctgtgcaa cgcttgggct tgtcttttta ggaggctcct atatggcaga atctttccga
    19501 agtgggctgg aagccatcag tcaaacccag caggagattg gcctcgctat tggtctgaca
    19561 cctctacagg tctttcgcta tgtggttctt ccgcaagcaa cagcggtggc actcccctcc
    19621 tttagtgcca atgtcatttt ccttatcaag gaaacctctg ttttctcagc agtggctttg
    19681 gccgacctca tgtacgtcgc caaggatttg attggtctct actatgagac agacattgcg
    19741 ctagctatgt tggtagttgc ttatctgatc atgctgctac ccatctcact ggtctttagc
    19801 tggatagaaa ggaggctccg ccatgcagga ttcgggaatc caagtactct ttcaaggaaa
    19861 taatctcctg agaatcttac agggattggg cgttacgatt gggatatcca tcctgtctgt
    19921 cctcttatcc atgatgttcg gaacagtcat gggaatcatc atgacctccc attctagaat
    19981 catacgattt ttaacacgat tgtatctgga atttatccgt atcatgcccc agctggtgct
    20041 actcttcatc gtttactttg gcttggctcg aaactttaat atcaatacct caggtgagac
    20101 ttcagctatt atcgttttta ccctctgggg aacagctgaa atgggagact tggtacgtgg
    20161 agctatcact tctctcccta aacatcagtt tgaaagtgga caggcactcg gcttgactaa
    20221 tgttcaactt tactaccaca tcatcatccc acaagtctta agaagactgc taccgcaggc
    20281 tatcaatctt gtcactcgga tgattaaaac cacttcatta gttgttttga ttggggttgt
    20341 ggaagtg
//
LOCUS       DAVDWM010000038        20221 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVDWM010000038 DAVDWM010000000
VERSION     DAVDWM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20221
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.038"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..562)
                     /locus_tag="VUW49_001583"
     CDS             complement(<1..562)
                     /locus_tag="VUW49_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7981479.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGY"
     gene            complement(729..881)
                     /locus_tag="VUW49_001584"
     CDS             complement(729..881)
                     /locus_tag="VUW49_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981480.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            complement(1012..1215)
                     /locus_tag="VUW49_001585"
     CDS             complement(1012..1215)
                     /locus_tag="VUW49_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981481.1"
                     /translation="MYIRLKNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSF
                     LKQLAKLYQVTTDYILEMDKFSC"
     gene            1717..2912
                     /locus_tag="VUW49_001586"
                     /pseudo
     CDS             1717..2912
                     /locus_tag="VUW49_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3291..4076)
                     /locus_tag="VUW49_001587"
     CDS             complement(3291..4076)
                     /locus_tag="VUW49_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981482.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(4078..4896)
                     /locus_tag="VUW49_001588"
     CDS             complement(4078..4896)
                     /locus_tag="VUW49_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET7981483.1"
                     /translation="MTKLWKRYKPFVSAGIQELITYRVNFFLYRIGDVMGAFVAFYLW
                     KAVFDSSHQSLIQGFTLSDMTLYIIMSFVTNLLTKSDSSFMIGWEVKDGSIIMRLLRP
                     VHFAMSYLFTEIGSRWLVFVSVGLPFVILIAGLKLLSGESFLQIVLITTVYLLSLILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFLSGSLVPLTFFPKIIAELLSFLPF
                     SSLIYTPVMVIIEKYSMSQMIQALSLQLFWLFIMIALSQLIWKCVQNYITIQGG"
     gene            complement(4898..5890)
                     /locus_tag="VUW49_001589"
     CDS             complement(4898..5890)
                     /locus_tag="VUW49_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7981484.1"
                     /translation="MKMITVEHLQKNFVKTIKEPGLKGAFRSFFHPKRQVFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQENRQGYVKDIGVV
                     FGQRTQLWWDLALQETYSVLKEIYDVPDAVFQKRMDFLNEVLDLKEFIQDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLG
                     DIEQLCDRIFMIDKGREIFDGTVNQLKKTFGKMKTLSFELHPGQDYIVSHFEGLSDIY
                     VTRQELSLDIQYDSSQYQTADIIQQTLSDFTIRDLKMTDANIEDIIRRFYRKEL"
     gene            complement(6907..7674)
                     /locus_tag="VUW49_001590"
     CDS             complement(6907..7674)
                     /locus_tag="VUW49_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET7981485.1"
                     /translation="MNNLTLLKEYNFRDLGNHLTQTGQKIKPKTLFRSSKLFGISKID
                     VDLLQSYGITKVIDFRSANEIKKAPDPDIKNIKNIVIPIFYNDDSELTEFPIEFFNKS
                     DAGFQHMIKTYDQMINQKQSKLGYKKFFRLLLSHPKDESLLFHCSMGKDRTGIASLFL
                     LYILGVDMNDIFHDYLLSNKYLINVRKENIEYVNNHSGNVILMHNLLSLSSAKEEYIN
                     RVLNVLDKEYGGILRYINTELGISSQEIEELKDRYLF"
     gene            complement(7775..9412)
                     /locus_tag="VUW49_001591"
     CDS             complement(7775..9412)
                     /locus_tag="VUW49_001591"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7981486.1"
                     /translation="MSIFIGGAWPYANGSLHIGHAAALLPGDILARYYRQKGEEVLYV
                     SGSDCNGTPISIRAKKENKSVKEIADFYHKEFKETFEKLGFTYDLYSRTDSPLHHEIV
                     QELFLQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRGDQCDNCSAIL
                     DPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRWRKNAINLTK
                     RYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDWAQKLQNNIT
                     DFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEPLPEYIISSEYLTLENKKISTSN
                     NWAIWLNDIIKKYDADSIRYFLTINAPEMKDANFSWREFIYSHNSELLGSYGNFINRT
                     LKFIEKYFESEIPTKYLEGEILYNLKELYTTVGNLVESGHMKQALEEIFEYIRSANKF
                     YDDMKPWALRESDIEKCKEVLATCVIIILNLGQMLNPFIPFSGKKIEDMFKTKLNTWN
                     YISNLPNKLSDVSMLFDRIDLKKIDEEVLELQQTSSR"
     gene            complement(9480..10115)
                     /locus_tag="VUW49_001592"
     CDS             complement(9480..10115)
                     /locus_tag="VUW49_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HET7981487.1"
                     /translation="MTFPISEWKRNEEFDFFNKMDVPYLSMSSRIEVTQAINFHKKHS
                     ISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTSEDKLNFSSFTMGD
                     NLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVSTPMNLSKIDTVPRI
                     TWGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVLLQRFVNKINEYFQKK"
     gene            complement(10112..11470)
                     /locus_tag="VUW49_001593"
     CDS             complement(10112..11470)
                     /locus_tag="VUW49_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981488.1"
                     /translation="MKLSSDLNILIRQLSYFIKDKEMLQQKIFGNFLDRFEEAYEQHF
                     FDIFDSLTGGIKISDVDWILGEEKLIKFLGRKNKNGQYLYVLPTNDGYLLRGSETYYH
                     YLSNVPYDYFKLIDKNIFIDGLKSTQNFLTEFIQYINSENSLTLKISLAFLDNLRNQI
                     LILLNAKFLVENDFKHGKYYVNFDSKLFKAIELFYSYYEKLFNFKHFILQPEDLVVIL
                     DILNSEIEALIPKLKNLDNNKVRKLTRVFRELDSIWEIFISLKYFFESENSLDVDNIS
                     EFCGIAYGGIEIPLVAHLFKEKNEISFLFQNSHYSTQEVEVKRFRDSRRNDSKSILLM
                     DDNILTGRAMKNAAQKLSYRKYSVQFFHVRRLGLNRLSQVIQENSMNELQRYLTGIYK
                     GGIFPAPYSKIKFGTNIAKQYLDELQIFTLSGDEILRLLYKNGLFSEESEVKVVRGNL
                     YE"
     gene            complement(11513..13246)
                     /locus_tag="VUW49_001594"
     CDS             complement(11513..13246)
                     /locus_tag="VUW49_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7981489.1"
                     /translation="MNELCKKYVYQLQNELNQYCDNLEGIHVIAYGSNGSNDLSISSD
                     FDVCLIVNDDSFVAMNQRLICDFTKLFHFQENLPIDKDIPLSNKLIYLSSEVELFLDN
                     PPFISDSKKVKIEDIKKTKNFLTSRTMKQRLLLNILTTDSTVLFGDEDYVNNLKYRAW
                     EDIEKIIERAYNIKLTTKNENEFLNLLYQNPVSKASGEYYLGYKKEKRNVVKHLERNV
                     HRLAILNEKKVEDIIDLSENVNRLLLKDYSFLFPSETIKKMISTYPEKKSCSIKEVLS
                     SKLTIASNNFLITNGSLEGMNIIIANLVQNNIGYLTPTFWGYGDSAKRYNKSVISAPL
                     SDIYNYNVTEIDKISKSCELMIICNPNNPTTATLTKNQIENLLLNNPQTYFLIDESML
                     PYKYDFFNYTMIPFVEKYTNLIVCSSISKSLCAPGLRIGYICANEKIVKKLEKKVIPF
                     NVSNINQCIAEKILENFPLSLSICSDIESSFRQLDLFMEKYTTQKSEKACGFRLYQLK
                     AKYDGNKIIDILTERGFLIRNISASYPELGENWIRISNGTLSEIKDLMHIMKDIFEDF
                     DDKASDSYDKG"
     gene            complement(13239..14711)
                     /locus_tag="VUW49_001595"
     CDS             complement(13239..14711)
                     /locus_tag="VUW49_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HET7981490.1"
                     /translation="MSKELYNKFILTKEAGNIIYDGIEFGRDEIDRRILEISKILTAD
                     IVNLSQEAVAISCNKKINFFFLFLVVNCCDGVVMPVYARTPENKLQHICERYDINYMV
                     SDHEINLVGYSLIYHGSVFSEEYFIYECNKSFPKHEELKGIELLMLSSGTTGVSKAIS
                     LSLDNILSNTRSIQKYIIPTREDRCLIIKEITHSSSLISELINSLLSKNSVLLSDLIP
                     TVSSLGYLISTYGVTILYAIPDFLLFMNSIKFINNYRQLLGKLRLVQVVGAFCPPNYI
                     TDFHKNWPKIDLFNGYGLTEASPRVAVLGGEELYANPRCVGYPISGVGVHIDTSSNNT
                     QEGEICVTGPNIMVEYYLNEELTSKIKIGGMLRTGDIGYFDDKNRLYILGRKDNRYIK
                     SGKNIFPEEIESVVRKLPFISEVIVYPKIESGRLISILEYTICNDEEKNLNQEASEII
                     KLCKDNLEDYKIPDQYRKVKNIPHTSTGKVKRDRNNYYNE"
     gene            complement(14715..16220)
                     /locus_tag="VUW49_001596"
     CDS             complement(14715..16220)
                     /locus_tag="VUW49_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET7981491.1"
                     /translation="MDNENEISKFFLSSENVKDMKTIANDILNMGLDFKLSEKVSQKK
                     MKEHDDLNFLSLEESIDNLPQLLEEFREKVIPYCTNFSSPNFMGFPDSGNSLGGLSGS
                     LLSDLLQQNLINASFCAPVATIMEINVIQWLRKVLGYSTSDVHNIMEVGGIVTYGGTG
                     SNSTAMLLARENKDGNTLELGVRNPRNYKVIVPKGIGHYSIASSLKWLGLGNNIIEVP
                     TENYRYNLKKLREALINHSNEIMAVVAYAGDSRTMTIDNLSEVADLVKTLTPNVWLHC
                     DACHGFSLAFSKKYKDRIKGIEKFDSISTDPHKVLGIPYCISVLLVKTPQSLNLIHSQ
                     SDLIMNENLALGQTTPFLGSKSWISLKLWLVAKHLGSEKIGAMVDKRIELTNYLQDLI
                     LKSSKLILLNRTDINSVMFMYIGDKHQEVSLSSVQNINNINKSIYDRLKDEGIYYLHQ
                     FPIIDNTETIYRGFELCPLRFMSGNPNLTIEELQKMVIEVENLGDRFVGES"
     gene            complement(16236..17426)
                     /locus_tag="VUW49_001597"
     CDS             complement(16236..17426)
                     /locus_tag="VUW49_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981492.1"
                     /translation="MMPQITQFLNNIKNNNEYYSNIINDATVLDDIPILNKNDIRLNP
                     DSIISECYSKKDLIHETTNGTTDYHPLDLYKTPQERVDLNFSLWKERRKFISASNIKS
                     VKIAVFYYSHDSLMNHDGSLKKVSGLIQVPMNRADDNQYIADLKYLIEEEIKWLIGPV
                     SIFLKYAKICKKYCIKFNVEYCECTSEYVPEVYRKEIEDVFKCKFLMQYSCHELWGIA
                     FTNSLEMYNVLKPLDNSIVEGLLRKEFTSKKLYSPVVTSLTLKAMPFIKYLLTDLVYF
                     DNGYIIPFGFRYTDMVKLKTDTIHCSIFDNLFGTLPFKFEPLEQYQIIYDAQNSEIVV
                     NVLNLEVTQGDIVCSVLKEYIKEHYKSSIKVSLRNCSKFFRDNLTGKMRGIINKENIN
                     NEIW"
     gene            complement(17423..17680)
                     /locus_tag="VUW49_001598"
     CDS             complement(17423..17680)
                     /locus_tag="VUW49_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7981493.1"
                     /translation="MDIRKKIIELCIKINPHLEDHYFYDYEKMIDDLDFDSLTITQLV
                     IDIEEQFDIMLPPQKISMSEMETFGKIVDLVHKCITSKESR"
     gene            complement(18032..19081)
                     /locus_tag="VUW49_001599"
     CDS             complement(18032..19081)
                     /locus_tag="VUW49_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981494.1"
                     /translation="MIELKIGKPSFGFLPYESHIEGILTGNNLNKIDYYLSMLDMEAI
                     CLQYGNKRIYDFFIGRNIQCNNIYREYSSRIDLSSEEALIPFLVDALAKGSFCYLSVD
                     TFCINKYISYKKEHIVHSPLVSGIDLDRNLIKISDFFDFTNYGIVWVPINEFIKGYFA
                     IQKVVEEGDIEETENWILKIEKINICKNKTEICDRMAINKVFKGYVLGSEEVKDSMDT
                     FSYINYRLANHKVFQKQSIVEQNLSVTGIKVYEYLKLYIEESIKEPQVLMRIKNLVLL
                     KKHFDVYKKIVQVLGINSEKVQACIEKAQLFLLIGMKINRSNQINNKLYFDMISIVRE
                     LKEMEYEIISEILSM"
     gene            19311..20125
                     /locus_tag="VUW49_001600"
                     /pseudo
     CDS             19311..20125
                     /locus_tag="VUW49_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT         6786 a         3700 c         2701 g         7034 t
ORIGIN      
        1 cataccctaa gcacccttga aaaccaacca attcaccacg ttgcatcttc atttttaatc
       61 cttttttcac atgggcagaa gtattctcta cttcttgctg tgcaacggag cttagaatag
      121 ttagaagcaa ttctccatcc attgttaggg tatctatgtt ttcttcctca aaatatactc
      181 caatttgcat atcctttaac attctgacat acttcaaagt atctagggta ttccttgcaa
      241 atctagcaat tgcttttgtg acaatataat ctatctctcc atttgcacaa tcattgatta
      301 atcgttgaaa atcttgtctc tttcctactt gtgttcctga aataccttca tctgcataaa
      361 tatctgccag ctcccaatct gaacgctgag aaatatattc tttgtagtgt cttacttgag
      421 aatcataact gtttttctga tcctcactat cggaactaac tctgcaataa gcagcaaccc
      481 tctttaagtg tctttctaca ttgtcgcctc ttcgacgaac taaagaatca gcttttatta
      541 tttcaacatt attttttttc atactgaaat tcctcctttt gattctatat taccgcattc
      601 aaatcaaaaa gtcagctatt ttgtgtactt aatacgcagt ttgtttctaa tcttgtcata
      661 ctcattttta gataacatat ttcttgagta taaaaagtta agcattgata gttgcatagt
      721 atactttatt actgattgtt ccaaactttt tcctccttgt aacatgtata atttcctccg
      781 tgggtatgac tggttaaatc attcatgggc atttcacccg ctgtcctttt tcgctggcag
      841 cctgaacgtt cagaagtatc attatcctta tttgtagtca tggcagatag agaaccactc
      901 tattttatag ctaataatta tcgctcctat cacggcgtat caagctaaag tgctcgacaa
      961 ataagaaagt cccacctcgg tttattcagt tttcaaaggg cttaggattt attaacaact
     1021 aaacttatcc atctctaaaa tataatctgt agttacctga tataatttag ctaattgttt
     1081 caaaaatgat gtaggaatat cttgcttgcc actttctatt ctagaataag cagactgtga
     1141 acaatgaaga tactgtgcaa tttgacactg ggacaaatct ttgtcttctc ttaaattctt
     1201 taatctgata tacattaata atcctcttac taatattgta atttgaagat tatcaaattt
     1261 ggaattttat gcaaaatatg cataaaataa tttataagat tttagaaata gttggtataa
     1321 cagtaagtaa gctataagtt actaaaaaaa gattttggaa taaacttttt catagcgata
     1381 aaagcaccgt acaagctagg aaaataataa gtaatcgccc aatctgacct tctccagaac
     1441 ttgattattc gtcaatcact acagttgaca aacatactaa gattagtagt gaggaaatct
     1501 ccaacgggag agagtactca ttactttttc tttgtgttaa aatagtggtg tcttgttaag
     1561 tcatagagct ctctatggtg ctagacgtcg cttgacaaac tggcaagaaa cctgttttag
     1621 gaagaatttt atcaaagtat gtgggatact agacgtcgag tattggaaat aaaatcacta
     1681 aaacaaggag tcattcaaga caaaggggta aacatgatgc ggacagtttt cagaatggat
     1741 gtgagtaagg caagctcaga agtcgctatt cttgtgaatg gtgaaaaggt tcatggctat
     1801 accatgccca atgatgccat cggatttagt cgtcttctag aggatttgaa ataggtaaat
     1861 acacctgaga ttatcttcga ggcacagggg tctattctcg tcttcaggcc tttctggagg
     1921 acaatagcta tgcttatata cggctaaatc ctttaaaact aagaagcaac tagacagttt
     1981 gcgagtccgt aaaaccaatc agattgacgc tgaaaagcta gcttcgtcac aattcatcct
     2041 taaccgcaaa ccaacttatg ttcaagaaga agtctatcaa aatttgcgtg acctcagtcg
     2101 attctatcag aatctgacag aagacatcgt taggactaag aatagcctgc acaaggtgct
     2161 tcaaattgcc tttccagaaa taaagaacct tctctctact cctacagggg aacaatattg
     2221 gaacctcgtg accatctttc caaccaagca ttggaggctg aaattatcag tagttgagct
     2281 aaaagaagcc atcgtaattc aacttctaaa cggatttctg agaacagagt tggacgattg
     2341 gctgaaaagt tgagcgagct ggccgaacag tcctatcttg ccatttcaaa aacctctccc
     2401 atgacagaag aggttaagta tcatgcacag gaactgttga gactgacaaa ccgcaaggat
     2461 caggacttag cacaaatgat tgaattggct gaaccattac ctgaatacga gattctgctc
     2521 tctattcctg gtatcgcaga aacaacagct acaagcatta ttggcgaatt ggagacattc
     2581 gtcgctttca gtctgccaac caaatcaatg cctttatcag tattgacctc agacactatg
     2641 aatctggcaa tttcctagct caagaacata tcaccaagcg tggaaatcct tacgcgagaa
     2701 aaattctcta taagtgcgtc cacaatattg cttcagctag tcataccaat ccttgtcata
     2761 tcgcggactt ttatgagaaa cgaaaaagac aatcgcagac gacttcaacc aagccatata
     2821 cgattgtctc tatacatcgc ctcatccgga caatgtatta cctcataacg cataacaaac
     2881 tttacgacta cactttaacc caaagtcatt aaagtcatct atgtcctatc attgtaacac
     2941 cttatttttt aataccagat aagtgtcttt taagtgtgcc caaatttcaa aaaacagctc
     3001 gcaattatct tctaaacatt cctttgatac tcattcagaa aaccttttga gctagaatat
     3061 cgtatgtccc ttaacaaatg gtatgtataa aagagctaaa aaaccactca tgatatgaac
     3121 gagtggttat gactgaaata ttacatacct ttgtatcatt tttgcacaat ggtcaaattt
     3181 cttgtttgaa aaactacagt aagcctaatc aaagctctca atgtatcatt aaaacttcct
     3241 tccaaagaat aggaagttct atcaaaattc ttaattagat tttacttctt ctaagaccct
     3301 gcgctctcat atgcatccaa acctttgttc cataatttta atgataaggt aaaaaatatt
     3361 aatgagataa ggatcaatcc accaatattg aacaatccat ccttgtcttt caagaaatag
     3421 ctagctgggt aataggctgt aaaagcaaaa ggaatgataa aactaatcaa ccaacgtaaa
     3481 aacgaatggt aaatcgcaat cgggtatttt gcaaaatcat taaacatgta aaaaatataa
     3541 attattgccc cagattgttt agtccaaaaa gcaatactag ctgttacaat ttttaaagag
     3601 gtataaatta aggttgcaaa gggaatactg attaaaaaga gaaaaacctt tgcccaagtc
     3661 caagtaagac tagttattgt cattaacagc aataagacgc ctactaacaa ttctccaaga
     3721 gcatcaattt gaaacgtttc tactaaaata tgaaacaaag ggctaatggg tctagtcaaa
     3781 tacttatcaa attctccttt acgaattaaa tgttgtccta aagcccacag attatcaaaa
     3841 aagagatggt caattccctt aggaatgaga gaaaaaccat aaataaaagc aacctgatga
     3901 aaactccagc catcaagtaa gggaatatgt tgaaacaaaa tatttagaaa cagcatgttt
     3961 aacccttgtg tcaaaaagac acccacaaca ccaactaaaa aatcagcctt atactccata
     4021 atctgtttaa gatattgtcg gataaaaatc aggtgcatgc gttgatattt cttcattcta
     4081 acctccttga atagttatat aattctgtac gcacttccaa atcagttggg ataaggcaat
     4141 cataataaac agccagaata attgtagcga gagcgcctga atcatttgac tcatgctata
     4201 cttttctata ataaccatca ccggggtata gattaaagaa gaaaatggca aaaaagataa
     4261 tagttcggca ataatttttg gaaaaaatgt cagaggaacc aaacttccag ataaaaaggc
     4321 aactaaagaa tttttcaaaa gatttgatcc ccataagttt ttaaacacaa aagctgaaaa
     4381 accaaagcaa atattgaaaa aaaaattaat caaaaaagct aagattaagc taagaaggta
     4441 aactgtggtt attaaaacaa tttgtaagaa ggactcacct gataagagct ttagacctgc
     4501 aatcagaatt acaaatggta gaccaacact aacaaaaact agccatctag acccaatttc
     4561 tgtgaaaaga taagacattg caaaatgaac tggacgaagc agtcgcataa taatagagcc
     4621 atcctttact tcccatccaa tcataaaaga actatctgat tttgtcagta gattcgtcac
     4681 aaaactcatg atgatatata aggtcatatc agataaagta aaaccttgaa tgagagactg
     4741 gtgagatgaa tcaaacacag ctttccacaa gtaaaatgct acaaaggccc ccatcacatc
     4801 cccaatgcga taaagaaaaa aattgacacg atacgtgatt aattcttgga ttccagcgct
     4861 aacaaaaggc ttatagcgtt tccataattt agtcatccta aagctccttt cgatagaagc
     4921 gacgaataat atcttcaata ttcgcatccg tcatctttaa atcacggatc gtaaaatcag
     4981 acaaagtttg ttgaataatg tcagccgttt gatactgcga gctgtcgtac tgaatatcaa
     5041 gtgacaattc ctgtctcgtc acatagatat cagaaagtcc ttcaaaatga gagacaatat
     5101 aatcctgccc aggatgtagt tcaaaagaga gagtcttcat cttcccgaaa gtctttttca
     5161 gttgattcac agttccatca aagatttctc ggcccttatc aatcataaaa atacgatcac
     5221 ataactgttc aatatcacct aaatcgtggg tcgttaaaag tatggtcgtc tcttcttctt
     5281 gattaatctg tgtaatggca cgtcgaatat tatccttgac cgaaacatct aaaccaatag
     5341 taggctcatc taggaaaaga actttcggat tatgcaataa ggaggccgca atatccgccc
     5401 tcatacgttg acctagagat agagttctga caggatcttg aataaattcc ttcaaatcta
     5461 aaacttcatt tagaaaatcc atgcgttttt gaaagaccgc atcaggaaca tcataaattt
     5521 cctttaaaac cgagtaagtc tcctgcaaag ccaaatccca ccaaagttga gttcgctgcc
     5581 caaaaactac accaatatct ttaacatagc cttgacggtt ctcttgagga atctttccat
     5641 tgatacgaca aaaacctgat gttggtttca aaatccccgt caacatcttg atagttgttg
     5701 atttccccgc tccatttgcg ccaataaagc ctaaaatttg tcctttgggc acctcaaatg
     5761 ataaatcttt aacagcttca aagacttgtc tcttgggatg aaaaaaagag cgaaaagctc
     5821 cctttaagcc aggttccttt attgttttca caaaattttt ctgaagatgc tctactgtta
     5881 tcatttttat aaatcactcc tttcgtttca ctcaagttat aatatcaaca aaaattgtag
     5941 catactacag taaagttaag aaaatttacc atcattacaa accatatgaa aaaagctact
     6001 aaaactatgc catcttgtat tttatctgta agtaagctaa attactcaag tacaaagaat
     6061 tatgacgaag aacatataaa tttataattg gataaactat agatttcagc aaacgaagtt
     6121 atcccaatat aaaaatactt taataactga agatattgac aggaaaatat taaattcctc
     6181 attactatcc tttactatgc agtataattt taatatcatt tacctacttc caagatgctg
     6241 aaaaatacta agtcacacta ttatgatgtt attatttcca tttttatttt aacagatgag
     6301 tcaattcttt caactacccc ctcccttata actatattca tgaagtattc ttgataataa
     6361 acctcattta acttctcaaa gtagtcagct tctgcaactg ctattatctc ataacagatt
     6421 gatttggttt caataaatgg aagctagaag ccttgtcagc taccctgcac agttggtgtt
     6481 ttttcatttt tctcttcaaa aaaattggtc ggaagttata ataaaaaggt gatgtgtttg
     6541 tggtgagttg aattaagaga caaactcact ttgtcttaac ttttaaattg cctaacaaaa
     6601 atacggagat tattctctaa gctttaactg caacattatg tctctacaac caacgataag
     6661 tccagaaatc tacccaaata accaaacaac ctacatttgt tgaactttga tgacaagtag
     6721 gaaattttac atttgataaa cactattttg tacgcatttt gcacataatt taacgtgctt
     6781 tccatcaatt tagttaagga cttacataga aaacaatccg tttttgctta tgttaaatta
     6841 gtagaattag acaaaaacaa aaccgccgtt cgcactgtta tatattcatt tttagtactt
     6901 actattctaa aaaagatatc tatctttcaa ttcctcaatt tcttgactac ttattcctag
     6961 ttctgtatta atatatctaa gtattcctcc atattcttta tcaaggacat tcaatactcg
     7021 attaatatat tcttcttttg ctgaactgag tgaaagtaaa ttatgcatca atattacatt
     7081 cccactatgg ttatttacat attcaatatt ttcttttctc acatttatta aatacttgtt
     7141 agatagtaag taatcatgaa aaatatcgtt catatctact cccaatatat aaagcaagaa
     7201 caagctagct attcctgttc gatccttccc cattgaacaa tgaaacaaaa gagattcatc
     7261 ttttggatga cttagtaata atctaaagaa tttcttgtaa ccaagtttac tctgtttttg
     7321 attgatcatt tgatcataag tttttatcat atgttgaaaa cctgcatctg atttgttaaa
     7381 aaattctatt gggaattctg tcaactctga atcatcatta tagaatattg gaattactat
     7441 attttttata ttttttatat ctggatctgg agcttttttt atttcattcg ctgatcgaaa
     7501 gtcaatgact tttgttattc catagctctg taacaaatcg acatcaattt tagagattcc
     7561 aaatagttta cttgaacgga ataatgtttt aggttttatt ttttgtcctg tttgagtgag
     7621 gtggtttcca agatctctaa aattatattc ttttagtaaa gttagattgt tcaatgcttt
     7681 tctccataat atgctcaaaa aaccatcttt attttattca acatctccgg ttgttaattt
     7741 aaattaaaaa aggctacttt cgagtatttt tgaattatct actggatgtt tgttgcaatt
     7801 ctaaaacttc ctcgtcaatt tttttcaaat caatcctatc aaacaacatt gatacatctg
     7861 atagcttgtt aggaaggttt gaaatataat tccaagtatt tagctttgtt ttaaacatat
     7921 cctcaatctt tttaccagaa aaaggaatga atggatttaa catttgtcct aaattaagta
     7981 taatgattac acaagtagct aagacttctt tacatttttc aatatcggac tctcgcaatg
     8041 cccacggttt catatcatca tagaatttat tagccgatct aatatattca aaaatttctt
     8101 ctaatgcctg tttcatatgc ccgctctcaa ctaaattacc tactgttgta tataattctt
     8161 tcaaattata taaaatttct ccttcgagat atttagtggg tatttcagat tcaaaatact
     8221 tttcaataaa ctttagagtc ctattgataa aattaccgta cgatcctaat aattctgagt
     8281 tatgactata aataaattct ctccaagaaa aatttgcatc tttcatctca ggagcattta
     8341 ttgttaggaa gtatcggatt gaatcagcat catatttttt tatgatatcg ttaagccata
     8401 tagcccaatt attacttgtt gatatttttt tgttttctaa cgttaaatat tcactagaaa
     8461 taatatactc tggaagaggc tctatttcta aacctgagag tattgccggc caaataatag
     8521 tatgaaatgg gatgttatct tttccatgaa cataatatga ctttgtccga ttgttccaaa
     8581 aatcagtaat attattttga agtttttgcg cccaatcaac acttgctgtg tagtaacctg
     8641 ctaccgcttc aaaccagaca tatatttttt tatctctaaa accatcaatg ggaactggaa
     8701 tcccatttgg tagatcacga gtaacagctc gatcaggtaa accttcccgt aaataccttt
     8761 tagttagatt tattgcattt tttcgccatc tcactgtttc ttcagcatca tttaagtaag
     8821 tctctaacaa attttgaaac tcactaaaga cataataaaa gtgttctgtc tcacgaactt
     8881 ctggttcatt ggaacaaata gaacatcttt tatcaactaa atctatagga tctaaaattg
     8941 ctgagcaatt atcacattga tctcctcttg aatgggttcc acaattcggg cattttcctt
     9001 caacgaacct atcaggtaag aattgattat caaatgtaca atagagttgt ttaatttttt
     9061 ttgtatacaa aaatttcttc tcgtataatt gcaaaaataa ttcttgtact atctcatgat
     9121 gtagtggaga atctgttcgc gagtacaagt catatgtaaa acctaatttt tcaaaagttt
     9181 ctttaaattc cttatgataa aaatcagcaa tttctttcac agacttattt tcttttttag
     9241 ctctgataga aataggggtt ccattacaat cacttccaga aacatataaa acttcctctc
     9301 ccttctgacg atagtatctt gcaagaatat cccccggtaa aagcgctgcc gcgtgaccaa
     9361 tatgtaacga accgtttgca tatggccatg ctcctccaat aaaaattgac atattgtatt
     9421 tctccttaaa ttctttattt ttattataac attatttaaa tgcaatatct tttcaatcat
     9481 tacttttttt gaaaatattc atttatttta tttacaaacc tttgcaataa cacaaaaaaa
     9541 ttagatacgt gtaatccatc tcccattcca tgattaattt gtaaattcac tgtacaaaaa
     9601 tatctttggt tctcttgaat aacttttccc cacgttattc gaggaactgt atcaattttt
     9661 gaaagattca taggagtgct tacatgtaaa aaatcaatcc atggaacaca agaaagataa
     9721 gcaatgttat tcttgtcaat atttggtttt tgcccttctt ctgccttctg tttatttatg
     9781 ttgaacgcag aaacaaattc aattaaatta tctcccatcg taaatgaaga aaaattaagc
     9841 ttatcttccg atgtaagggt agtgaaagaa caacctcttt gattgtattg ccagacaatc
     9901 tttccgtcgg tatccattag taattctgga atagaattta ttgcactcat aacacaccac
     9961 gatattatgg cataaagtga tatcgaatgt tttttatgaa aattgatagc ctgcgttact
    10021 tctattcgtg aactcataga taaataagga acatccattt tattgaaaaa gtcaaattct
    10081 tcattccttt tccactctga tataggaaat gtcattcgta taaattcccc cttaccactt
    10141 tcacttcact ctcttctgag aacaagccat tcttataaag tagtcttaag atttcatctc
    10201 cagatagagt aaaaatttgt aattcatcta aatattgttt agcaatatta gtaccgaatt
    10261 taatttttga atacggtgca ggaaaaatcc ctcctttata tattcctgtt aaatacctct
    10321 gcaattcatt cattgaattt tcttgaataa cttgtgataa tctatttagg ccaagcctcc
    10381 tgacatgaaa gaattggact gaatattttc ggtatgataa tttctgagca gcatttttca
    10441 tagcccttcc tgtcaaaata ttatcatcca ttaataagat acttttcgaa tcatttctcc
    10501 gactgtccct gaaccttttt acttcaactt cttgagtact gtagtggcta ttttgaaata
    10561 aaaatgatat ttcatttttt tctttaaata ggtgcgcaac caagggaatt tctatcccgc
    10621 cataagctat cccacaaaat tcactaatat tatctacatc caatgaattt tcactttcaa
    10681 agaaatactt taaagaaata aaaatttccc agatagaatc taattcccta aaaacacgag
    10741 taagctttct aactttatta ttatctagat ttttaagctt aggaatcaaa gcttcaattt
    10801 cagagtttaa aatatcaagg atcacaacta aatcttccgg ttgcagaata aaatgcttga
    10861 aattaaatag cttttcataa tagctgtaaa ataactcaat agctttgaat agtttagaat
    10921 cgaaattcac ataatatttc ccatgtttaa aatcattctc tactaaaaac ttagcattca
    10981 agagtattaa aatttgattt cttaaattgt ctagaaatgc aagactgatt ttaagagtca
    11041 aagagttttc tgaatttata tattggatga attccgttaa aaaattttgt gtagatttca
    11101 agccatcgat aaatatattt ttgtcaatga gtttaaaata atcatatggt acattcgata
    11161 aataatggta gtatgtttca gaacctctga gtaaatagcc gtcatttgtt ggaagtacgt
    11221 agaggtattg accattttta ttttttctcc caagaaattt aatcaacttt tcttcaccta
    11281 aaatccaatc aacatcacta atcttaattc ccccagtaag tgaatcaaaa atatcaaaaa
    11341 agtgctgctc ataagcctct tcaaatctat ctaagaaatt accaaaaatc ttttgttgca
    11401 acatttcctt gtcttttata aaataagaga gttgccttat taaaatattt aagtcactac
    11461 ttaatttcat attattattc aaaatctccg cactatcttt tattttcaac acctacccct
    11521 tgtcataact atcactggct ttgtcatcaa aatcttcaaa gatatctttc attatgtgca
    11581 tcaaatcttt aatctctgat aacgtgccat tactaatcct tatccaattt tcacccaatt
    11641 caggatatga agcacttata tttcttatca aaaaacctct ttccgttagt atatctatta
    11701 ttttattacc atcatatttt gctttcaact ggtataacct gaaaccacac gccttttcag
    11761 acttttgagt agtgtatttc tccataaata aatctaattg tctaaatgaa gactcaatgt
    11821 ctgaacatat acttagagag agcggaaaat tttctaatat tttttcagct atacattgat
    11881 tgatattaga aacattgaaa ggaattactt ttttttctaa tttttttaca attttttcgt
    11941 tagcacatat atagcctatt cttaatccag gagcacatag tgacttcgat attgaggaac
    12001 aaacaatcaa attagtatat ttttctacaa atggtatcat tgtataatta aaaaaatcat
    12061 atttatatgg caacatgctt tcatcaatca aaaaatatgt ttgtggattg tttaaaagaa
    12121 gattttctat ttgatttttt gttaaagtag ccgtagtcgg attatttggg ttacatatta
    12181 tcataagctc acaagattta gaaattttat ctatttctgt aacattataa ttataaatat
    12241 ctgacaaagg tgccgaaatc actgatttat tatacctttt tgcagaatcc ccatatcccc
    12301 aaaaagtagg ggttaaatat cctatattat tttgaaccaa gttcgcaatt ataatattca
    12361 taccctctaa agaaccgttt gtaatcaaga aattgttact ggctattgtt aatttagaag
    12421 acaaaacttc tttaatgctg catgattttt tttctggata tgtgcttatc atttttttta
    12481 tagtttcaga aggaaataaa aatgagtaat ctttcaagag taacctattg acgttttcag
    12541 ataaatcaat tatatcttcc acctttttct cgttcaaaat tgctaatcgg tgaacattcc
    12601 tttctaaatg tttaacaaca ttcctttttt cttttttata tcctaaataa tactctccac
    12661 ttgctttcga tactgggttt tgatatagta aatttaagaa ttcattttca tttttggtcg
    12721 ttaatttaat attatacgct ctttcaatta ttttttcaat gtcctcccat gccctatatt
    12781 tcagattatt aacataatct tcatcaccaa ataaaactgt agaatctgta gtcaaaatat
    12841 ttagtaagag acgttgtttc atagttcttg aagttaaaaa gttcttcgtt tttttaatat
    12901 cttcaatctt aactttctta gaatctgata tgaacggcgg attatccaaa aataattcaa
    12961 cctcactgct tagataaata agtttgttgc tcaaaggaat atccttatct atcggtaaat
    13021 tttcttgaaa atgaaataac tttgtaaagt cacatatcaa ccgctgattc atagcaacaa
    13081 atgaatcatc gttaacaata agacaaacat caaaatcaga tgaaatagac aaatcattac
    13141 taccatttga accgtaagca ataacatgga ttccctccag attatcacaa tactgattta
    13201 gttcgttttg taattgataa acatattttt tacatagctc attcattata ataattattc
    13261 ctatcacgtt ttactttccc tgtagaagtg tgcgggatat ttttaacctt cctatattga
    13321 tcagggattt tataatcttc tagattatct ttgcataatt ttattatttc acttgcttct
    13381 tgattcaaat ttttctcttc atcattgcaa atagtatatt caagaataga tattaatctc
    13441 ccagattcta ttttaggata aactattact tcggatataa aaggtaattt tcttactaca
    13501 gattctattt cttccggaaa tatatttttt cctgatttta tatacctatt gtcttttctg
    13561 cctaaaatat ataacctgtt tttatcatca aagtaaccaa tgtctccagt ccttaacata
    13621 cctccaattt ttattttaga agtaagctct tcgttcaagt aatattcaac cataatatta
    13681 ggaccagtaa cacaaatttc tccctcttgg gtgttgttgc tacttgtatc aatatgcaca
    13741 ccaactcctg aaattggata acctacacac ctaggatttg catataattc ttctcctcct
    13801 aatacagcaa ctctaggaga ggcctctgtt aaaccataac cattaaataa atcaattttt
    13861 ggccagtttt tatgaaaatc tgttatataa tttggaggac aaaatgcacc tacaacctgt
    13921 actaatctta atttccctaa caattgtcta taattattta taaatttaat agagttcata
    13981 aacaatagaa aatcaggtat agcgtataaa atagtcacac cgtatgtact tattaaatac
    14041 cctaatgaac taacggtcgg aattaaatcg cttaacaaca ctgaattttt tgataacaaa
    14101 ctatttatta attctgatat caatgacgac gagtgtgtga tttcttttat tatgagacac
    14161 ctatcttctc tagtaggaat aatatatttt tgaatgcttc tagtattaga taaaatatta
    14221 tcaagcgata aactaatcgc ttttgaaact cctgtagtac ctgaagagag cattaacaat
    14281 tcaatcccct tcaactcctc atgtttcgga aatgatttat tacactcgta tataaaatac
    14341 tcttctgaaa aaacactccc atgatatatt aaactatacc ctaccaaatt aatttcatgg
    14401 tcagaaacca tatagtttat gtcatacctt tcacagatat gttgtaattt attttctggc
    14461 gttctagcat acactggcat aacaacacca tcacaacaat taactaccag aaaaagaaag
    14521 aaaaagttta tttttttatt acaacttata gcaactgctt cttgtgataa gttgacaata
    14581 tctgcagtaa gtattttaga aatttcgaga attctcctat caatttcatc cctgccaaat
    14641 tcaatgccgt catatattat atttccagct tcctttgtca aaataaattt attatagagc
    14701 tctttactca ttttttaact ctctcctaca aatctatccc ctagattttc tacttctata
    14761 accatttttt gtagttcttc aattgttaaa ttaggatttc cagacataaa tcgtaatgga
    14821 cataattcaa accctctgta tatggtttct gtattatcta ttattggaaa ttgatgtagg
    14881 tagtatattc cttcatcttt tagtctgtca taaatactct tattaatgtt atttatattc
    14941 tgtactgatg ataagcttac ttcttgatgt ttatcaccta tatacatgaa cataactgaa
    15001 ttaatatctg tcctatttaa taatattaac ttagaactct taagtattag atcttgcaaa
    15061 taattcgtta actctattct cttgtctacc atcgctccta ttttctctga tcctaaatgt
    15121 ttagctacta accacaattt tagagatatc caacttttac ttcccaaaaa tggagtcgtt
    15181 tgcccaaggg ccaagttttc attcataatc aaatctgatt gagagtgaat tagattaagc
    15241 gattgaggtg ttttcactaa caaaacactt atgcaataag gaattcctaa aactttatgc
    15301 ggatctgtcg aaatactatc aaatttttca attcctttta tcctatcttt atacttttta
    15361 gaaaaagcta atgaaaatcc gtgacaagca tcacagtgta accaaacatt tggggtaagt
    15421 gtctttacca aatctgctac ttcagataga ttatcaatag tcattgttct actatctcca
    15481 gcatatgcaa ccactgccat tatttcatta gaatggttga ttagtgcttc cctaagtttc
    15541 ttcaagttat agcgataatt ttctgtagga acttcaataa tattattccc caatcccagc
    15601 cactttaaac ttgatgcgat actataatga ccaattcctt ttggcacaat taccttatag
    15661 tttctggggt ttcttacgcc taattcaaga gtatttccat ctttattttc tcttgctaaa
    15721 agcatagctg tagaattact tcctgtacca ccataggtaa cgatgccgcc tacttccata
    15781 atattatgaa catcagaagt ggaatatcct aaaacttttc gtaaccactg gattacattt
    15841 atttccataa tagtagcaac gggagcgcaa aaagatgcat taatcaaatt ttgctgtaat
    15901 aaatcactaa gcaaactacc tgaaagaccg cctaatgaat ttcctgagtc aggaaaaccc
    15961 ataaaattag gggaagaaaa atttgtacag taaggaatta ctttttccct gaattcttcc
    16021 aaaagttgcg gaagattgtc tatactctct tctaagctga gaaaatttaa atcatcgtgt
    16081 tctttcattt ttttctgaga aactttttcg cttaatttaa aatccaaccc catatttaaa
    16141 atgtcatttg cgatagtttt catatctttt acattttctg aactcagaaa aaactttgaa
    16201 atttcgtttt cattatccat gtatgagttt gcctcctacc aaatttcatt atttatattc
    16261 tctttattta ttatcccacg catttttcct gtcagattat ctctaaaaaa ttttgaacaa
    16321 tttcgaagag aaacttttat tgaacttttg tagtgttctt tgatatattc ttttaaaacc
    16381 gagcaaacaa tatcaccttg tgtcacttct aaatttagaa cattaaccac aatctctgaa
    16441 ttttgagcat cgtagataat ctgatactgt tctaaaggtt caaatttaaa tggtaatgtt
    16501 ccaaataaat tatcaaatat tgaacaatgg atggtatctg tttttagttt taccatgtct
    16561 gtgtatctaa agccaaacgg gataatatac ccattatcaa agtaaaccaa atcagtaagt
    16621 aaatatttta taaatggcat tgcctttaga gttagtgaag taacaactgg actatataat
    16681 tttttagaag taaattcttt tctcagcaga ccttcaacta tagaattatc taatggttta
    16741 agtacattat acatttctaa tgaatttgta aatgcaattc cccataactc atgacagcta
    16801 tattgcatta aaaatttaca tttaaataca tcttctattt cctttctata tacttcaggg
    16861 acatactcac tagtacattc acaatattca acattaaatt taatacaata ttttttacaa
    16921 atctttgcat atttcaagaa tatcgaaacc ggaccaatca gccattttat ttcttcctca
    16981 ataagatatt ttaaatccgc aatatattga ttatcgtcag ccctattcat tggaacttga
    17041 atcaatcccg atactttttt cagactgcca tcatgattca ttaatgaatc atgactgtaa
    17101 taaaaaactg ctatcttaac agacttaata ttagaagcac taataaattt tcttctttct
    17161 ttccaaagtg aaaagttcaa gtcaactctt tcttgaggag tcttgtataa atccaatgga
    17221 tgataatctg tcgtgccatt agttgtttca tgtataagat cttttttgct gtaacattct
    17281 gaaataatag aatctgggtt taatctaata tcattcttat tcaaaattgg aatatcatct
    17341 aaaactgtgg catcattaat gatgtttgaa taatattcat tgttgttttt tatattattt
    17401 aaaaattgtg tgatttgagg catcatctac tctccttgct cgtgatacat ttatgtacca
    17461 agtcaacaat ttttccaaac gtttccattt ctgacataga aattttttgt gggggaagca
    17521 ttatgtcaaa ttgttcttca atatctataa ctaattgcgt gattgtcaga gaatcaaaat
    17581 ctaaatcatc aatcattttt tcataatcat aaaaataatg atcttctaaa tgcggattaa
    17641 tcttaatgca taattcaata attttttttc taatatccat atttttacct ttctcaaagt
    17701 ttgtaatttg gttgtggagt taattgttgt taattataac ccaatataat atttgtgcta
    17761 ttttgtaaag tacacactaa tagaaatatt tttccaaaat acagttaaat ctcaatataa
    17821 ttaaagtaca aaaaagccta caaaagtgaa ctttttgact tttctaattt gcttctttta
    17881 ttacctcgtc ccaaatcaca aaactacaga tttagagaga ggttccccat ggctctataa
    17941 tccttctaaa aaaatctaat cttatatctt cgaattatct ttcaaaaatc cactattaga
    18001 ttccaaagat ttctcttaaa aattcaatat tttacattga caaaatctca ctgataattt
    18061 cgtactccat ttcttttaat tcccttacta tgctaatcat atcaaagtat aatttattgt
    18121 tgatttgatt agatctattt attttcatac caattagcaa aaacaactga gccttttcaa
    18181 tacacgcttg caccttttca gagttaatac ctaacacttg aactattttt ttataaacat
    18241 caaaatgttt ttttaataat acaagatttt taattctcat cagaacttga ggttctttaa
    18301 tactttcttc aatataaagt ttcaaatatt catatacttt tattcctgtc acactaagat
    18361 tttgctctac tatactctgt ttctgaaaaa ccttatgatt agctaaccta taatttatat
    18421 aactaaatgt atccattgaa tccttcactt cttctgaacc caaaacatag cctttaaaaa
    18481 ctttatttat cgccattcta tcacaaattt ctgttttatt tttacatata tttatttttt
    18541 ctattttcag tatccagttt tctgtttctt caatatcacc ttcttccacc accttttgaa
    18601 ttgcaaaata tcctttaatg aactcattta taggaaccca aacaattcca taatttgtaa
    18661 aatcaaaaaa gtcagatatt tttatcaggt ttctatctaa atcaattcct gaaaccaaag
    18721 gtgaatgcac aatatgttcc tttttataac taatatactt attaatacag aatgtatcta
    18781 ctgataaata acaaaaagaa cctttagcta acgcatcaac caaaaaaggg attaaagctt
    18841 cctcactaga taaatctata cgtgacgaat actctctgta tatattatta cattgtatat
    18901 ttcttccaat gaaaaaatca taaattctct tattaccata ttgaagacat atcgcttcca
    18961 tatccagcat ggataaataa taatcaattt tatttaggtt atttcctgta agaattcctt
    19021 caatatgtga ttcatagggc aaaaagccaa agcttggttt tccaatcttt aattcaatca
    19081 tttttataaa tctcctaata taaatttact ttcatcgaac taaactcaaa ttgacataat
    19141 ttctgtcatc attcctgctt taattcacaa ctattcttat attatgcaaa aacttatacc
    19201 tgattaacgc aataatataa atttagagac taaccaagat gaattcgtcc tttttaacaa
    19261 tttattcagt agacttgttc ggtaactgta aaaagtgtta tactattttt atggaaacag
    19321 tatacgacaa agcacaaaaa cttaactcaa aaaacttcaa actattgatt ggtgtcaaaa
    19381 aggaaacctt tcaactcatg ctagaacacc tgaattcagc ctatcagatt cagcaccgaa
    19441 aaggtggacg tccacgtagt ctgcccatgg aagaccagct cattatgacc ctccgttact
    19501 tgcgatatta tcccactcag cgtctgctgg cctttgattt tggcgtcggt gtagctacgg
    19561 taaatgccat catcacttgg gtggaggata cacttcgtgc gtcaggtagc tttgatttgg
    19621 accatttaga agccccgagt gctgctgtgg ctattgacgt gaccgaaagt ccgattcagc
    19681 gtccaaaaaa aaccaaagca aaaattattc tggtaaaaag aaacgacaca ccttaaaaac
    19741 tcaaattatg ctggatttga cgacacataa agtctgtcaa atggcctttt ctgacggaca
    19801 tacgcatgat tttactctct tcaaagaaag tattggacaa agtttgcctg aaacgacgct
    19861 tgcctttgtt gacctaggtt atttaggcat cttgaaattt catgagaata ctttcattcc
    19921 tgctaaaaat tccaaaaatc gccgcctgag tgaggatgat aagcagttaa ataaagagat
    19981 gtcagcgata cgaattgaaa ttgaacattt taacgctaaa ttcaagacct tccaaatcat
    20041 gtcagtccct tatcgtaact gcagaaaacg tttcgagtta cgggcggaat taatttgtgc
    20101 catcatcaat tatgaagtga actagattcc gaacaagtct agtataattt taccacaaag
    20161 taacatgatt tgcaagcgat ttcaataata aagatatatg attatcctca ggaataaata
    20221 a
//
LOCUS       DAVDWM010000039        19701 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVDWM010000039 DAVDWM010000000
VERSION     DAVDWM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.039"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(183..818)
                     /locus_tag="VUW49_001601"
     CDS             complement(183..818)
                     /locus_tag="VUW49_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET7981495.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            874..2172
                     /locus_tag="VUW49_001602"
     CDS             874..2172
                     /locus_tag="VUW49_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7981496.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLHIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            2169..2831
                     /locus_tag="VUW49_001603"
     CDS             2169..2831
                     /locus_tag="VUW49_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7981497.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            2911..3459
                     /gene="raiA"
                     /locus_tag="VUW49_001604"
     CDS             2911..3459
                     /gene="raiA"
                     /locus_tag="VUW49_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7981498.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            3595..5568
                     /locus_tag="VUW49_001605"
     CDS             3595..5568
                     /locus_tag="VUW49_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7981499.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            5565..6017
                     /gene="rplI"
                     /locus_tag="VUW49_001606"
     CDS             5565..6017
                     /gene="rplI"
                     /locus_tag="VUW49_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7981500.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            6061..7413
                     /gene="dnaB"
                     /locus_tag="VUW49_001607"
     CDS             6061..7413
                     /gene="dnaB"
                     /locus_tag="VUW49_001607"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7981501.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            7415..7681
                     /locus_tag="VUW49_001608"
     CDS             7415..7681
                     /locus_tag="VUW49_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981502.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            7770..9116
                     /gene="cbpD"
                     /locus_tag="VUW49_001609"
     CDS             7770..9116
                     /gene="cbpD"
                     /locus_tag="VUW49_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET7981503.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      9199..9301
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9372..9662
                     /locus_tag="VUW49_001610"
     CDS             9372..9662
                     /locus_tag="VUW49_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET7981504.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETPH"
     gene            9625..10383
                     /locus_tag="VUW49_001611"
     CDS             9625..10383
                     /locus_tag="VUW49_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981505.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLISCLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            10422..11429
                     /locus_tag="VUW49_001612"
     CDS             10422..11429
                     /locus_tag="VUW49_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7981506.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            11429..12157
                     /locus_tag="VUW49_001613"
     CDS             11429..12157
                     /locus_tag="VUW49_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981507.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            12276..12734
                     /locus_tag="VUW49_001614"
     CDS             12276..12734
                     /locus_tag="VUW49_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET7981508.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            12736..15168
                     /locus_tag="VUW49_001615"
     CDS             12736..15168
                     /locus_tag="VUW49_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7981509.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            15489..16142
                     /gene="rr06"
                     /locus_tag="VUW49_001616"
     CDS             15489..16142
                     /gene="rr06"
                     /locus_tag="VUW49_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET7981510.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDEITQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            16139..17470
                     /gene="hk06"
                     /locus_tag="VUW49_001617"
     CDS             16139..17470
                     /gene="hk06"
                     /locus_tag="VUW49_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET7981511.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            17553..18080
                     /locus_tag="VUW49_001618"
     CDS             17553..18080
                     /locus_tag="VUW49_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET7981512.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            18167..>19701
                     /gene="pspC"
                     /locus_tag="VUW49_001619"
     CDS             18167..>19701
                     /gene="pspC"
                     /locus_tag="VUW49_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HET7981513.1"
                     /translation="MFASKNERKVHYSIRKFSVGVASVAVASLFMGSVVHATEREGST
                     PVATSSNKANKSQTEHMKAAEQVDEYINKMIQLDKRKHTQNLALNIKLSAIKTKYLRE
                     LNVLEEKSKKEELTSKTKKEIDAAFEQFNKDTLKPGEKVEEAQKKVEEAEKKAKDQKE
                     EDHRNYPTITYKTLELEIAESDVEVKKAELELVKEEAKGSRNEEKIKKAKAEVESKKA
                     EATKLEEIKTERKKAEEEAKRKAEAEEEVKDKLKKRTKRGALGEPATPDKKENDAKSS
                     DSSVGEETLPSPSLKSEKKVAEAEKKVAEAEKKAKDQKEEDRRNYPTNTYKTLELEIA
                     ESDVKVKEAELELVKEEAKEPQNEEKIKQAKAKVESKKAEATRLEKIKTDRKKAEEAK
                     RKVAEEDKVKEKPAEQPQPAPAPKPAPAPQPEKPAEQPKAEKPADQQAEEDYARRSEE
                     EYNRLTQQQPPKAEKPAPAPQPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNG
                     SWY"
BASE COUNT         6271 a         3191 c         4529 g         5710 t
ORIGIN      
        1 atcaaagtca ttaattttca agtgattgcg ttcagctaat tctagtaaat aggtgtgaag
       61 gaaaacgata tcaggtttcg tttgaattgc ttcattttct agtatatttt ggtacgcatc
      121 tttttatttt gctcgctaaa gtagttgtga gaatgtcaac gaaacgctgt attattcatt
      181 gtttacacta agtttacagg aacttcaacc tctcgtaaac cttggtcagt taaagtgact
      241 tttccattaa aaaactccac aagtgcagct ttaatagttt ctttttcttc tttatcaaca
      301 taaatcatcg tatcgacttg atctgtaaag tttgtatcca gctccatgag atcatgttct
      361 ttaaggaagt tactgtactc ttggtactga gcataagaca tttgaatagc aatgccagcc
      421 tgttctttta tttcaataat accaatttct ttgacagcta aggcgacact gccggcgtaa
      481 gcacgaatta gtcctccagc gcctagttta ataccaccaa agtagcgtgt cacgaccaca
      541 cagacattgg tgagattgtg attttctagt accccaagca tgggaacacc agcagtacca
      601 ctaggctcac catcatcact tgtacgttta atttcactac gttctccaat aataaaggca
      661 gagcagttat gtgtcgcttt gtagtgttct tttttgatgg cagtaatgaa gtcacgagcc
      721 tcttcttcgc tataaacacg cttggcatgg cagataaagc gagatttttt gatttcttct
      781 tggacttgac cgtcctcttt aattgttcta aattccatga tttaattgta ctaaaaaatc
      841 atctaaagtg ctagttttta cgaataaaga agtatgaaag taaatttaga ttatctcggt
      901 cgtttattta ctgagaatga attaacagaa gaagaacgtc agttggcgga gaaacttcca
      961 gcaatgagaa aggagaaggg gaaacttttc tgtcaacgtt gtaatagtac tattctagaa
     1021 gaatggtatt tgcacatcgg tgcttactat tgtcgagagt gcttgctgat gaagcgagtc
     1081 agaagtgatc aaactttata ctattttccg caggaggatt ttccgaagca agatgttctc
     1141 aaatggcgcg gccaattaac tccttttcaa gagaaggtgt cagagggact gcttcaagca
     1201 gtagacaagc aaaagccaac cttagttcat gcggtaacag gagctggaaa gacagaaatg
     1261 atttatcaag tagtggctaa agtgatcaat gcgggtggtg cagtgtgttt ggctagtcct
     1321 cgcatagatg tttgtttgga gctgtataag cgcctgcaac aggatttttc ttgcgggata
     1381 gctttgctac atggagaatc ggaaccttat tttcgaacac cactagttgt tgcaacaacc
     1441 catcagttat tgaagtttta tcaagctttt gatttgctga tagtggatga agtagatgct
     1501 tttccttatg ttgataatcc cacgctttac catgctgtca agaatagtgt aaaggagaat
     1561 ggattgagaa tctttttaac agcgacttcg accaatgagt tagataaaaa ggtccgttta
     1621 ggagaactaa aaagactgaa tttaccgaga cggtttcatg gaaatccgtt gattattcca
     1681 aaaccaattt ggttatcgga ttttaatcgc tacttagaca agaatcgttt gtcaccaaag
     1741 ttaaagtcct atattgagaa gcagagaaag acagcttatc cgttactcat ttttgcttca
     1801 gaaattaaga aaggggagca gttagcagaa atcttacagg agcaatttcc aaatgagaaa
     1861 attggctttg tatcttctgt aacagaggat cgattagagc aagtacaagc ttttcgagat
     1921 ggagaactga caatacttat cagtacgaca atcttggagc gtggagttac cttcccttgt
     1981 gtggatgttt tcgtagtaga ggccaatcat cgtttgttta ccaagtctag tttgattcag
     2041 attggtggac gagttggacg aagcatggat agaccgacag gagatttgct tttcttccat
     2101 gatgggttaa atgcttcaat caagaaggcg attaaggaaa ttcagatgat gaataaggag
     2161 gctggtctat gaagtgcttg ttatgtgggc agactatgaa gactgtttta acttttagta
     2221 gtctcttact tctgaggaat gatgactctt gtctttgttc agactgtgat tctacttttg
     2281 aaagaattgg ggaagagaac tgtccaaatt gtatgaaaac agagttgtca acaaagtgtc
     2341 aagattgtca actttggtgt aaagagggag ttgaagtcag tcatagagcg atttttactt
     2401 acaatcaagc tatgaaggat tttttcagtc ggtataagtt tgatggagac ttcctgttaa
     2461 gaaaagtttt cgcttcattt ttaagtgagg agttgaaaaa gtacaaagag tatcaatttg
     2521 ttgtaattcc cctaagtcct gatagatatg ctaatagagg atttaatcag gttgagggct
     2581 tggtagaggc agcaggcttt gagtatctgg atttattaga gaaaagagaa gagagagcca
     2641 gttcttctaa aaatcgttca gagcgcttgg ggacagaact tcctttcttt attaaaagtg
     2701 gagtcactat tcctaaaaaa atcctactta tagatgatat ctatactaca ggagcaacta
     2761 taaatcgtgt taagaaactg ttggaagaag ctggtgctaa ggatgtaaaa acattttccc
     2821 ttgtaagatg aggaaaaaat ttgcaaaaat agaatgaaag cgttataata aaatcataaa
     2881 aacaaaaatg tttagaaaga aggtactcat atgattaaat atagtatccg tggtgaaaac
     2941 ctagaagtaa cagaagcaat tcgtgattat gtagtttcta aactcgaaaa gatcgaaaag
     3001 tacttccaac cagaacaaga gttggatgcc cgaattaact taaaagttta tcgtgaaaaa
     3061 acggctaaag tggaagtaac gattccgctt ggatctatta ctctccgcgc agaagatgta
     3121 tctcaagata tgtatggttc aattgacctt gtaactgata aaattgaacg tcagattcgt
     3181 aaaaataaaa caaaaatcga gcgtaaaaat aaaaataagg tagcaactgg tcaattattt
     3241 acagatgctt tggtggaaga ttcaaatatt gtccagtcta aagttgttcg ttcaaaacaa
     3301 attgatttaa aaccaatgga tttggaagaa gcaattctac aaatggattt attggggcat
     3361 gatttcttta tctatgtgga tgttgaagat cagacaacca atgtgattta tcgtcgtgag
     3421 gatggcgaaa ttggtttgtt agaggttaaa gaatcttaat ttcaatatgt gtaaaggtag
     3481 gtttactgaa ttgtaaacta cctttttctt ataattgata gaattactga ttacactttt
     3541 aaaaagtcgc tttttgaggg gtattatggt ataatgggtg ccaagaggtt ttgaatgaaa
     3601 aaattttatg taagtccaat ttttcctatt ctagtaggat tgattgcgtt tggagtctta
     3661 tccactttca ttatttttgt taataataat ctgttgacgg ttttaatttt gtttcttttt
     3721 gtaggaggct atgttttttt atttaagaaa ctgagagtgc attatacaag gagtgatgta
     3781 gaacagatac agtatgtaaa ccaccaagcg gaagaaagtt tgacagctct attggaacag
     3841 atgcctgtag gtgttatgaa attgaattta tcttctggag aggttgagtg gtttaatccc
     3901 tatgctgaat tgattttgac caaggaagat ggtgattttg atttagaagc tgttcaaacg
     3961 attatcaagg cttcagtagg aaatccgtct acttatgcca agcttggtga gaagcgttat
     4021 gctgttcata tggatgcttc ttccggtgtt ttgtattttg tagatgtatc cagggaacaa
     4081 gccataacag atgaattggt aacaagtaga ccagtgattg ggattgtctc tgtggataat
     4141 tatgatgatt tggaggatgc aacttctgag tcagatatta gtcaaatcaa tagttttgta
     4201 gctaatttta tatcagagtt ttcagaaaaa tacatgatgt tttctcgtcg ggtaagtatg
     4261 gatcgatttt atctatttac tgactacacg gtgcttgagg gcttgatgaa tgataaattt
     4321 tctgttattg atgctttcag agaagagtcg aaacagagac agttgccctt gaccttaagt
     4381 atgggatttt cttatggcga tggaaatcat gatgagatag ggaaagttgc tttgctcaat
     4441 ttgaacttgg ctgaagtacg tggtggcgac caggtggttg ttaaggaaaa cgacgaaacg
     4501 aaaaatccag tttattttgg tggtgggtct gctgcttcaa tcaagcgtac acggactcgt
     4561 acgcgcgcta tgatgacagc tatttcagat aagattcgga gtgtagatca ggtttttgta
     4621 gtcggtcaca aaaatttaga catggatgct ttgggctctg ctgtaggtat gcagttgttc
     4681 gccagcaatg tgattgaaaa tagctatgct ctttatgatg aagaacaaat gtctccagat
     4741 attgaacgag ctgtttcatt catagaaaaa gaaggagtta cgaagttgtt gtctgttaag
     4801 gatgcaatgg ggatggtgac caatcgttct ttgttgattc ttgtagacca ttcaaagaca
     4861 gccttaacat tatcaaaaga attttatgat ttatttaccc aaaccattgt tattgaccac
     4921 catagaaggg atcaggattt tccagataat gcagtcatta cttatatcga aagtggtgca
     4981 agtagtgcca gtgagttggt aacggaattg attcagttcc agaattctaa gaaaaatcgt
     5041 ttgagtcgta tgcaagcaag tgttttgatg gctggtatga tgttggatac taaaaatttc
     5101 acctcgcgag taactagtcg gacatttgat gttgctagct atctcagaac gcgcggaagt
     5161 gatagtattg ctatccagga aattgctgcg acagattttg aagaatatcg tgaggttaat
     5221 gaacttattt tacaggggtg taaattaggt tcagatgtac taatagcaga ggctaaggac
     5281 acgaaatgct atgatacagt tgttattagt aaggcagcag atgccatgtt agccatgtca
     5341 ggtattgaag cgagttttgt tcttgcgaag aatacacaag gatttatctc tatctcagct
     5401 cgaagtcgta gtaaactgaa tgtacaacgg attatggaag agttaggcgg tggaggccac
     5461 tttaatttgg cagcagctca aattaaagat gtaaccttgt cagaagcagg tgaaaaactg
     5521 acagaaattg tattaaatga aataaaggaa aaggagaaag aagaatgaaa gtaatctttt
     5581 tagcagatgt taaaggaaaa ggtaaaaaag gcgaaattaa ggaagtacca acagggtatg
     5641 cgcaaaactt tcttatcaaa aagaatctag ccaaagaagc gactgctcaa gctgtaggtg
     5701 aacttcgtgg taaacaaaaa tcggaagaaa aagctcacgc tgagatgatt gcagaaggaa
     5761 aagcaattaa agcacaactt gaagcagaag aaactgttgt agaatttgtt gaaaaagttg
     5821 gtccagatgg tcgtaccttt ggttctatta ccaataagaa gattgcagaa gaattgcaaa
     5881 agcaatttgg aattaagatt gataaacgtc atattcaagt acaagctccg attcgagcgg
     5941 ttggtttgat tgatgtgcca gtgaaaatct atcaagatat cacaagtgta atcaatcttc
     6001 gtgtgaaaga aggataagtt tacaccttct tgacaagatt gtaaaaggaa gggaagtctg
     6061 atggcagaag tagaagagtt acgagtacaa cctcaagata tcttagctga gcaatccgtt
     6121 ttaggggcta tctttattga tgagagtaaa cttgtttttg tgcgagaata cattgagtct
     6181 cgggactttt ttaagtatgc ccatcgtttg attttccaag ccatggtcga tttatccgat
     6241 cgtggtgatg ccatagatgc aacaacggtt cgtactatcc ttgataatca aggtgattta
     6301 cagaatattg gtggcttgtc ttacttggtt gagattgtta attctgtgcc aacttctgct
     6361 aatgcggagt attatgctaa gattgttgca gaaaaagcaa tgctacgtcg tttaattgcc
     6421 aagttgacag agtctgtcaa ccaagcttac gaagcgtcac aaccagctga tgaaattatt
     6481 gctcaggcag aaaaagggtt gattgatgtc agtgaaaatg caaatcgaag cgggtttaag
     6541 aacattcgag atgtgttgaa tctcaacttt ggaaatctgg aagctcgctc gcaacaaacg
     6601 accgatatta caggtattgc gacaggttat cgtgatttgg atcatatgac aacaggactt
     6661 catgaggagg agttgattat cttagcagct cgtccagcag ttggtaagac agcatttgcc
     6721 ttgaatatcg ctcagaatat tgggactaag ttggacaaaa cggttgctat tttttcactc
     6781 gaaatgggtg cggaaagctt ggtagaccgt atgttagctg cagaaggctt ggtggagtca
     6841 cattctatcc gtacagggca attgacagat gaggagtggc aaaaatatac tattgctcag
     6901 ggtaatctag ctaacgccag tatctatatc gatgatacgc caggtattcg gattacagag
     6961 attcgttctc gttctcgtaa attggctcaa gaaactggaa atcttggttt gattttgata
     7021 gactatttgc aacttatcac gggaactggt cgagaaaatc gtcaacaaga agtttctgaa
     7081 atttctcgtc agttgaaaat actagccaag gaattgaagg ttccagtaat cgctctgagt
     7141 cagctttctc gtggtgtaga acaacgtcag gacaagagac cggtcttgtc tgatattcgt
     7201 gaatctgggt ctattgagca ggacgctgat atcgtagctt ttctctatcg cgatgactac
     7261 tatgaacgtg gtggtgaaga agaggagggt atcccaaata ataaggtgga agttattatc
     7321 gagaaaaacc gtagtggagc tcgtggaaca gtggaattga ttttccaaaa agaatacaat
     7381 aaattttcaa gtatctcaaa gagggaggca taagatgtca gatgcattta cagatgtagc
     7441 caagatgaaa aaaatcaaag aagaaatcaa ggcacatgag ggacaagtcg tagaaatgac
     7501 tttggagaat ggtcgtaagc gccaaaaaaa tagattgggt aagctaattg aagtttatcc
     7561 atccctattt attgtggagt ttggggatgt ggaaggagat aaacaagtta atgtttacgt
     7621 tgaatccttt acttactcag atattcttac agaaaagaat ttgattcatt atcttgacta
     7681 aagtgagaaa ttttctcact ttttcttttt tctccgaata atttaggtga aggcaatcat
     7741 cgctttatat tattttttaa ggaggaagaa tgaaaatttt accgtttata gcaagaggaa
     7801 caagttatta cttgaagatg tcagttaaaa agcttgttcc ttttttagta gtaggattga
     7861 tgctagcagc tggtgatagt gtctatgcct attccagagg aaatggatcg attgcgcgtg
     7921 gggatgatta tcctgcttat tataaaaatg ggagccagga gattgatcag tggcgcatgt
     7981 attctcgtca gtgtacttct tttgtagcct ttcgtttgag taatgtcaat ggttttgaaa
     8041 ttccggcagc ttatggaaat gcgaatgaat ggggacatcg tgctcgtcgg gaaggttatc
     8101 gtgtagataa tacaccgacg attggttcca ttacttggtc tactgcagga acttatggtc
     8161 atgttgcctg ggtgtcaaat gtaatgggag atcagattga gattgaggaa tataactatg
     8221 gttatacaga atcctataat aaacgaatta taaaagcaaa cacgatgaca ggatttattc
     8281 attttaaaga tttggatggt ggcagtgttg ggaatagtca atcctcaact tcaacaggcg
     8341 gaactcatta ttttaagacc aagtctgcta ttaaaactga acccctagtt agtgcaactg
     8401 tgattgatta ctattatcct ggagagaagg ttcattatga tcaaattctc gaaaaagacg
     8461 gctacaagtg gttgagttat actgcctata atggaagcta tcgttatgtt caattggagg
     8521 ctgtgaataa aaatcctcta ggtaattctg ttctttcttc aacaggtgga actcattatt
     8581 ttaagaccaa gtctgctatc aaaactgaac ccctagttag tgcaactgtg attgattact
     8641 attatcctgg ggagaaggtt cattatgatc agatacttga aaaagacggc tacaagtggt
     8701 tgagttatac ggcttataac ggaagtcgtc gctatataca gctagaggga gtgacttctt
     8761 cacaaaatta tcagaatcaa tcaggaaata tctctagcta tggatccaat aatagttcaa
     8821 ctgtcggttg gaagaaaata aatggtagtt ggtatcattt caaatcaaat ggttctaaat
     8881 caacaggatg gctgaaagat ggttctagct ggtattattt gaaattatct ggtgaaatgc
     8941 agacaggatg gttaaaggag aatggctcgt ggtattatct gggtagttca ggggcaatga
     9001 aaacaggctg gtaccaggtc tctggtaagt ggtattattc ttactcttca ggcgccttag
     9061 ctgttaatac gacggtggat ggctacagag taaacagtga tggagaacga gtatagaaaa
     9121 ttggagtagg agaaatttcc tgctcttttc tgtaagcagt ttccttgaca tttttcttat
     9181 tttgcgctat catatataca tataatatat gggagtctgt gtacagtctg agaggaagtg
     9241 ttaaacttcg accgcacctg atctgggtaa tgccagcgga gggaacgata cttagtctaa
     9301 ttttgcacct tttccatgta tggtaaaggt ttttcttttt ttaaaaagga aaacgagaag
     9361 aggaggttct tatgaaagca agcattgcct tgcaagtttt acccctagca caggggattg
     9421 atcggatagc tgttattgat caggtcattg cttatctgca aactcaagaa gtgacgatgg
     9481 tagtgacacc atttgaaacg gtcttggaag gggagtttga tgagcttatg cgcattctaa
     9541 aagaagcgct ggaagtggca gggcaggagg cagacaatgt ctttgccaat gtcaaaataa
     9601 atgtaggaga gattttaagt attgatgaga aacttgagaa gtatactgag acgccacatt
     9661 agtctattgg gctttctcgg agtcttgtca atctggcagt tagcaggttt tcttaaactt
     9721 ctccccaagt ttatcctgcc gacacctctt gaaattctcc agccctttgt tcgtgacaga
     9781 gaatttctct ggcaccatag ctgggcgacc ttgagagtgg ctttactggg gctgattttg
     9841 ggagttttga tttcctgtct tatggctgtg ctcatggata gtttgacttg gctcaatgac
     9901 ctgatttacc ctatgatggt ggtcattcag accattccga ccattgccat agctcctatc
     9961 ctggtcttgt ggctaggtta tgggattttg cccaagattg tcttgattat cttaacgaca
    10021 acctttccca tcatcgttag tattttggac ggttttaggc attgtgacaa ggatatgctg
    10081 accttgttta gtctgatgcg ggccaagcct tggcaaatcc tgtggcattt taaaatccca
    10141 gttagcctgc cttactttta tgcaggtctg agggtcagtg tctcctacgc ctttatcaca
    10201 actgtggtat ctgagtggtt gggaggtttt gaaggtcttg gtgtttatat gattcagtct
    10261 aaaaaactgt ttcagtatga taccatgttt gccattatta ttctggtgtc gattatcagt
    10321 cttttgggta tgaagctggt cgatatcagt gaaaaatatg tgattaaatg gaaacgttcg
    10381 tagaattaga atgtttctga aaaagaaaag aggaaatcaa aatgaagaaa acatggaaag
    10441 tgtttttaac gcttgtaaca gctcttgtag ctgttgtgct tgtggcctgt ggtcaaggaa
    10501 ctgcttctaa agacaacaaa gaggcagaac ttaagaaggt tgactttatc ctagactgga
    10561 caccaaatac caaccacaca gggctttatg ttgccaagga aaaaggttat ttcaaagaag
    10621 ctggagtgga tgttgatttg aaattgccac cagaagaaag ttcttctgac ttggttatca
    10681 acggaaaggc accatttgca gtgtatttcc aagactacat ggctaagaaa ttggaaaaag
    10741 gagcaggaat cactgccgtt gcagctattg ttgaacacaa tacatcagga atcatctctc
    10801 gtaaatctga taatgtaagc agtccaaaag acttggttgg taagaaatat gggacatgga
    10861 atgacccaac tgaacttgct atgttgaaaa ccttggtaga atctcaaggt ggagactttg
    10921 agaaggttga aaaagtacca aataacgact caaactcaat cacaccgatt gccaatggcg
    10981 tctttgatac tgcttggatt tactacggtt gggatggtat ccttgctaaa tctcaaggtg
    11041 tagatgctaa cttcatgtac ttgaaagact atgtcaagga gtttgactac tattcaccag
    11101 ttatcatcgc aaacaacgac tatctgaaag ataacaaaga agaagctcgc aaagtcatcc
    11161 aagccatcaa aaaaggctac caatatgcca tggaacatcc agaagaagct gcagatattc
    11221 tcatcaagaa tgcacctgaa ctcaaggaaa aacgtgactt tgtcatcgaa tctcaaaaat
    11281 acttgtcaaa agaatacgca agcgacaagg aaaaatgggg tcaatttgac gcagctcgct
    11341 ggaatgcttt ctacaaatgg gataaagaaa atggtatcct taaagaagac ttgacagaca
    11401 aaggcttcac caacgaattt gtgaaataat gacagaaatt agactagagc acgtcagtta
    11461 tgcctatggt caggagagga ttttagagga tatcaaccta caggtgactt caggcgaagt
    11521 ggtttccatc ttaggcccaa gtggtgttgg aaagaccacc ctctttaatc taatcgctgg
    11581 gattttagaa gttcagtcag ggagaattgt ccttgatggt gaagaaaatc ccaaggggcg
    11641 cgtgagttat atgttgcaaa aggatctgct cttggagcac aagacggtgc ttggaaatat
    11701 cattctgccc ctcttgattc aaaaggtgga taaggcagaa gctatttccc gagcggataa
    11761 aattcttgcg accttccagc tgacagctgt aagagacaag tatcctcatg aacttagcgg
    11821 tgggatgcgc cagcgtgtag ccttactccg gacctacctt tttgggcaca agctctttct
    11881 cttagatgag gcctttagcg ccttggatga gatgacaaag atggaactcc acgcttggta
    11941 tcttgagatt cacaagcagt tgcagctaac aaccctgatc atcacgcata gtattgagga
    12001 ggccctcaat ctcagcgacc gcatctatat cttgaaaaat cgccctgggc agattgtttc
    12061 agaaattaaa ctagattggt ctgaagatga ggacaaggaa gtccaaaaga ttgcctacaa
    12121 acgtcaaatc ttggcagaat taggcttaga taagtagaaa aatagggagt tggtgaagat
    12181 tatcctttac cagcgccctt tttcttttaa aaatgagaaa atttcggtat aatagtcaaa
    12241 caaggtcaag gtttaaagag agaggtgggt ttgttatgag atttaaaaat acatcggatc
    12301 atattgaggc ctacatcaag gcgattttag atcaatctgg tatcgtggag ttgcaacgga
    12361 gtcagttggc agataccttt caggttgttc ctagtcagat taactacgtg atcaagacac
    12421 gctttacgga aagtagaggc tacttggttg aaagtaagcg tggtggcgga ggctacattc
    12481 gtataggacg gattgagttt tctagtcatc atgaaatgct ccgggagctg ctttactcga
    12541 ttggtgagcg agtcagtcaa gaaatttatg aggatattct ccagcttttg gttgagcagg
    12601 aattgatgac caagcaggag atgaatttgc tagaatcagt agctttggat cgcgttttag
    12661 gagaagaagc tccagttgtt cgagcaaaca tgctacgtca ggtcatacaa gaggtagata
    12721 gaaaagggaa gtaagatgaa ctattcaaaa gcattgaatg aatgtatcga aagtgcctac
    12781 atggttgctg gacattttgg agctcgttat ctagagtcgt ggcacttgtt gattgccatg
    12841 tctaatcaca gttatagtgt agcaggggca actttaaatg attatccgta tgagatggac
    12901 cgtttagaag aggtggcttt ggaactgact gaaacggact atagccagga tgaaaccttt
    12961 acggaattgc cgttctcccg tcgtttgcag gttctttttg atgaagcaga gtatgtagcg
    13021 tcagtggtcc atgctaaggt actagggaca gagcacgtcc tctatgcgat tttgcatgat
    13081 ggcaatgcct tggcgactcg tatcttggag agggctggtt tttcttatga agacaagaaa
    13141 gatcaggtca agattgctgc tcttcgtcga aatttagaag aacgggcagg ctggactcgt
    13201 gaagatctca aggctttacg ccaacgccat cgtacagtag ctgacaagca aaattctatg
    13261 gccaatatga tgggcatgcc gcagactccg agtggtggtc tcgaggatta tacgcatgat
    13321 ttgacagagc aagcgcgttc tggcaagtta gaaccagtca tcggtcggga caaggaaatc
    13381 tcacgtatga ttcaaatctt gagccgtaag actaagaaca accctgtctt ggttggggat
    13441 gctggtgtcg ggaaaacagc tctggccctt ggtcttgccc agcgtattgc tagtggtgac
    13501 gtgcctgcgg aaatggctaa gatgcgcgtg ttagaacttg atttgatgaa tgtcgttgca
    13561 gggacacgct tccgtggtga ctttgaagaa cgcatgaata atatcatcaa ggatattgaa
    13621 gaagatggcc aagtcatcct ctttatcgat gaactccaca ccatcatggg ttctggtagc
    13681 gggattgatt cgactctgga tgcggccaat atcttgaaac cagccttggc gcgtggaact
    13741 ttgagaacgg ttggtgccac tactcaggaa gaatatcaaa aacatatcga aaaagatgcg
    13801 gcactttctc gtcgtttcgc taaagtgacg attgaagaac caagtgtggc agatagtatg
    13861 actattttac aaggtttgaa ggcgacttat gagaaacatc accgtgtaca aatcacagat
    13921 gaagcggttg aaacagcggt taagatggct catcgttatt taaccagtcg tcacttgcca
    13981 gactctgcta tcgacctctt ggacgaagca gcagcaacag tgcaaaataa ggcaaagcat
    14041 gtaaaagcag acgattcaga tttgagtcca gctgacaagg ccctgatgga tggcaagtgg
    14101 aaacaggcag cccagctaat cgcaaaagaa gaggaagtac ctgtctacaa agacttggtg
    14161 acagagtctg atattttgac caccttgagt cgcttgtcag gaatcccagt tcaaaagctg
    14221 actcagactg acgctaagaa atatctaaat cttgaagcag aactccataa acgggttatc
    14281 ggtcaagatc aagctgtttc aagtattagt cgtgcgattc gccgtaatca gtcaggaatt
    14341 cgcagtcaca agcgtccgat cggttccttt atgttcctag gacccacagg tgtcggtaag
    14401 accgagttgg ccaaggcttt ggcagaagtt ctctttgatg acgaatcagc ccttatccgc
    14461 tttgatatga gtgagtatat ggagaaattc gcagccagcc gtctaaatgg agctcctcca
    14521 ggctatgtgg gttacgaaga aggtggggag ttgacagaga aggttcgcaa taaaccctat
    14581 tccgttctcc tctttgatga ggtagagaag gctcacccag atatctttaa tgttctcttg
    14641 caggttctgg atgacggtgt cttgacagat agcaagggac gcaaggtcga tttttcaaat
    14701 accattatca ttatgacatc gaacctaggt gcgactgccc ttcgtgatga taagactgtt
    14761 ggttttgggg ctaaggatat tcgttttgac caggaaaata tggaaaaacg catgtttgaa
    14821 gaactgaaaa aagcttatag accggaattc atcaaccgta ttgatgagaa ggtggtcttc
    14881 catagcctat ctagtgatca tatgcaggaa gtggtgaaga ttatggtcaa gcctttagtg
    14941 gcaagtttgg ctgaaaaagg cattgacttg aaattacaag cttcagctct gaaattgtta
    15001 gcaaatcaag gatatgaccc agagatggga gctcgcccac ttcgcagaac cctgcaaaca
    15061 gaagtggagg acaagttggc agaacttctt ctcaagggag atttagtggc aggcagcaca
    15121 cttaagattg gtgtcaaagc aggccagtta aaatttgata ttgcataaaa gaataaaagt
    15181 accagcatct gaccataagt cacagtggag tgaaactcaa tgaaaatcaa agagcaaact
    15241 aggcagctag ccgcaggttg ctcaaaacac tgttttgagg ttgcagatag agctgacgtg
    15301 gtttgaagag attttcgaag agtataagct gcaagatgaa tgattttctt gtattgacgt
    15361 tgttgttgac aaaaagtagc ggataaatga aatccattct attatcatag atgataggct
    15421 ggtaggaaat tttcaaatag catacaggaa atagatgtat ggagttctgg tagtagaaag
    15481 ggagagagat gaacatttta gttgcagatg acgaggaaat gattagagaa ggaattgcag
    15541 catttctgac agaagagggt tatcatgtca ttatggctaa ggatggacaa gaggtcttgg
    15601 aaaaatttca agatctccct atccatctca tggtactgga tttaatgatg cctaggaaga
    15661 gtggttttga agtgttaaaa gaaatcaatc aaaagcacga tattcctgtc atcgtcttga
    15721 gtgctctggg agatgaaatt actcagtcac aggtatttga tctctatgct gatgatcatg
    15781 tgacaaaacc tttttctttg gtactgcttg tcaagcgtat taaggcgctt atcagacgtt
    15841 actacgtcat agaggatatt tggcgatatc aggatgtaac agtggatttt acctcttaca
    15901 aagcacatta taaaaatgaa gaaattgatc tcaaaccaaa ggaattactg gtactaaagt
    15961 gtttgattca gcataaaaat caagttttaa gtagagagca gatattggaa gaaatttcaa
    16021 aagatgtagc tgatttacct tgtgataggg tcgttgatgt ctatattcgt actcttcgca
    16081 aaaaattagc tttagattgt atcgtgactg tgaaaaatgt tgggtataag attagtttat
    16141 gataaaaaat cctaaattat taaccaagtc ctttttaaga agttttgcaa ttctaggtgg
    16201 tgttggtcta gtcattcata tagctattta tttgaccttt cctttttatt atattcaact
    16261 ggagggggaa aagtttaatg agagcgcaag agtgtttacg gagtatttaa agactaagac
    16321 atctgatgaa attccaagct tactccagtc ttattcaaag tccttgacca tatctgctca
    16381 ccttaaaaga gatattgtag ataagcggct ccctcttgtg catgacttgg atattaaaga
    16441 tggaaagcta tcaaattata tcgtgatgtt agatatgtct gttagtacag cagatggtaa
    16501 acaggtaaca gtgcaatttg ttcacggggt ggatgtctac aaagaagcaa agaatatttt
    16561 gcttttgtat ctcccatata catttttggt tacaattgct ttttcctttg ttttttctta
    16621 tttttatact aaacgcttgc tcaatcctct tttttacatt tcagaagtga ctagtaaaat
    16681 gcaagatttg gatgacaata ttcgttttga tgaaagtagg aaagatgaag ttggtgaagt
    16741 tggaaaacag attaatggta tgtatgagca cttgttgaag gttatttatg agttggaaag
    16801 tcgtaatgag caaattgtaa aattgcaaaa tcaaaaggtt tcctttgtcc gcggagcatc
    16861 acatgagttg aaaacccctt tagccagtct tagaattatc ctagagaata tgcagcataa
    16921 tattggagat tacaaagatc atccaaaata tattgcaaag agtataaata agattgacca
    16981 gatgagccac ttattggaag aagtactgga gtcttctaaa ttccaagagt ggacagagtg
    17041 tcgtgagact ttgactgtta agccagtttt agtagatatt ttatcacgtt atcaagaatt
    17101 agctcattca ataggtgtta caattgaaaa tcaattgaca gatgctacca gagtcgtcat
    17161 gagtcttagg gcattggata aggttttgac aaacctgatt agtaatgcaa ttaaatattc
    17221 agataaaaat gggcgcgtaa tcatatccga gcaagatggc tatctctcta tcaaaaatac
    17281 atgtgcgcct ctaagtgacc aagaactaga acatttattt gatatatttt atcattctca
    17341 aatcgtgaca gataaggatg aaagttccgg tttgggtctt tacattgtga ataatatttt
    17401 agaaagctat caaatggatt atagttttct cccttatgaa cacggtatgg aatttaagat
    17461 tagcttgtag acagattagt tttttattaa agttcatata gggttaacat aagtgtgtta
    17521 ttctttgtgt agataaaaga aaggatacta atatggtatt agcgattatt ttagtaacat
    17581 tctttattcg attgattttt ttaaagcgtt cgatagagaa tgagaaacga atccttagca
    17641 atggcgggaa agaatttgga gttgagaata caaaacgatt aactatggct catattgttt
    17701 tttatctctc ttgcttggtt gaggcaatgg tgcacaagac aatttttgat ggcatgggca
    17761 tggttggttt agtcttgctt attttttcta tgctgatgtt gatgttggtg attcacttgt
    17821 tgggagatat ttggacagtg aagcttatgc ttgtcaataa tcacaaatat gtagatcata
    17881 tcttgtttag gacagtaaaa caccctaatt actttttaaa tattttacct gagttgattg
    17941 gcttgacctt gttgagtcat gcctatatga cttttgtttt agtttttcca gtttatgcag
    18001 ttattttgta tcgacgaata gctgaagagg aaaagctatt acatgaagtt ataatcccaa
    18061 atggaagcat aaagagataa agagataaat acaaaattcg atttatatac agttcatatt
    18121 gaagtgatat agtaaggtta aagaaaaaat atagaaggaa ataaacatgt ttgcatcaaa
    18181 aaacgaaaga aaagtacatt attcaattcg taaatttagt gtaggagtag ctagcgtagc
    18241 tgttgccagt ctttttatgg gaagtgttgt acatgcgaca gagagagagg gaagtacccc
    18301 agtagccact tcttctaata aggcaaataa aagtcagaca gaacatatga aagctgctga
    18361 acaagtcgat gaatatataa acaaaatgat ccaattagat aaaagaaaac atacccaaaa
    18421 tctcgcctta aacataaagt tgagcgcaat taaaacgaag tatttgcgtg aattaaatgt
    18481 tttagaagag aagtcgaaaa aagaagagtt gacgtcaaaa acaaaaaaag agatagacgc
    18541 agcttttgag cagtttaaca aagatacatt gaaaccagga gaaaaggttg aagaagctca
    18601 gaagaaggtt gaagaagctg agaaaaaagc caaggatcaa aaagaagaag atcaccgtaa
    18661 ctacccaacc attacttaca aaacgcttga acttgaaatt gctgagtccg atgtggaagt
    18721 taaaaaagcg gagcttgaac tagtaaaaga ggaagctaag ggatctcgaa acgaggaaaa
    18781 aattaagaaa gcaaaagcgg aagttgagag taaaaaagct gaggctacaa agttagaaga
    18841 aatcaagaca gaacgtaaaa aagcagaaga agaagctaaa cgaaaagcag aagcagaaga
    18901 agaagttaaa gataaactaa agaagcggac aaaacgagga gctcttggag agccagcaac
    18961 acctgataaa aaagaaaatg atgcgaagtc ttcagattct agcgtaggtg aagaaactct
    19021 tccaagccca tccctgaaat cagaaaaaaa agtagcagaa gctgagaaga aggttgcaga
    19081 agctgagaaa aaagccaagg atcaaaaaga agaagatcgc cgtaactacc caaccaatac
    19141 ttacaaaacg cttgaacttg aaattgctga gtccgatgtg aaagttaaag aagcggagct
    19201 tgaactagta aaagaggaag ctaaggaacc tcaaaacgag gaaaaaatta agcaagcaaa
    19261 agcgaaagtt gagagtaaaa aagctgaggc tacaaggtta gaaaaaatca agacagatcg
    19321 taaaaaagca gaagaagcta aacgaaaagt agcagaagaa gataaagtta aagaaaaacc
    19381 agctgaacaa ccacaaccag ctcctgcacc aaaaccagcg ccggctcctc aaccagaaaa
    19441 accagctgaa caaccaaaag cagaaaaacc agctgatcaa caagctgaag aagactatgc
    19501 tcgtagatca gaagaagaat ataaccgctt gactcaacag caaccgccaa aagcagaaaa
    19561 accagctcct gcaccacaac cagagcaacc agctcctgca ccaaaaacag gttggaaaca
    19621 agaaaacggt atgtggtact tctacaatac tgatggttca atggcaacag gttggctcca
    19681 aaacaatggt tcatggtact a
//
LOCUS       DAVDWM010000040        19294 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVDWM010000040 DAVDWM010000000
VERSION     DAVDWM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19294
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.040"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            87..3437
                     /locus_tag="VUW49_001620"
     CDS             87..3437
                     /locus_tag="VUW49_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET7981514.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFGENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIEEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLGEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3708..3785
                     /locus_tag="VUW49_001621"
                     /pseudo
     CDS             3708..3785
                     /locus_tag="VUW49_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4023..4493)
                     /locus_tag="VUW49_001622"
     CDS             complement(4023..4493)
                     /locus_tag="VUW49_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET7981515.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQTELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4510..6783)
                     /locus_tag="VUW49_001623"
     CDS             complement(4510..6783)
                     /locus_tag="VUW49_001623"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET7981516.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHINKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7130..10231
                     /locus_tag="VUW49_001624"
     CDS             7130..10231
                     /locus_tag="VUW49_001624"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7981517.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            10314..11321
                     /gene="pfkA"
                     /locus_tag="VUW49_001625"
     CDS             10314..11321
                     /gene="pfkA"
                     /locus_tag="VUW49_001625"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7981518.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            11380..12885
                     /gene="pyk"
                     /locus_tag="VUW49_001626"
     CDS             11380..12885
                     /gene="pyk"
                     /locus_tag="VUW49_001626"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7981519.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(13577..13802)
                     /locus_tag="VUW49_001627"
                     /pseudo
     CDS             complement(13577..13802)
                     /locus_tag="VUW49_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(14006..14224)
                     /locus_tag="VUW49_001628"
     CDS             complement(14006..14224)
                     /locus_tag="VUW49_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981520.1"
                     /translation="MGTKIGISKNTIGNYEKRVKSTKKNTIFDLAKVFSSLIDALFPP
                     VQKDSPSDIQSIYDQRAPPRQGKVLTYA"
     gene            14660..15532
                     /locus_tag="VUW49_001629"
     CDS             14660..15532
                     /locus_tag="VUW49_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET7981521.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVL
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(15678..16022)
                     /locus_tag="VUW49_001630"
                     /pseudo
     CDS             complement(15678..16022)
                     /locus_tag="VUW49_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            16165..16476
                     /locus_tag="VUW49_001631"
     CDS             16165..16476
                     /locus_tag="VUW49_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981522.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            16480..17196
                     /locus_tag="VUW49_001632"
     CDS             16480..17196
                     /locus_tag="VUW49_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7981523.1"
                     /translation="MKKYFIGGLGNNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYLTSEFEEVKKLILLDGDYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(17332..18147)
                     /locus_tag="VUW49_001633"
     CDS             complement(17332..18147)
                     /locus_tag="VUW49_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET7981524.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(18144..19049)
                     /locus_tag="VUW49_001634"
     CDS             complement(18144..19049)
                     /locus_tag="VUW49_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET7981525.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
BASE COUNT         6019 a         3292 c         4129 g         5854 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttggagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcat
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttgttttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcagaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattga ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggataattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gccaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctgggt gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gcagataaga
     3781 ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta
     3841 accataccta cggcaaggcg acgttgacgc ggattgaaga gattttcgaa gagtataaat
     3901 cttcctagga taaagcaaaa cgcatagtat caagggtttt caacacttga tactatgcgt
     3961 tttctgatgt taaagacttt ctaccagttt ttttaagcat aattgttagt tgtagtcatt
     4021 tattattctt caaagaaaaa tggtggggcg aattttttca gttcttcaaa gcacttttga
     4081 gcagtatctg catcttcaca gatgataaga cagacatcat taccacaaag ggtagcgata
     4141 gcgtcaggga agctcaaagt atcaatgata gaaccaaagg attgagccag tccaggaagg
     4201 gtttttagta ggacttggtg ttgaactggg cgcatccaga caagggcgtc ttccatgtag
     4261 agttcgagac gtttttccca ttttgagatg gaaccattgt taagaacata ataagcgcta
     4321 tcttcttcgc ggacttttga taggttcata tttttgatgt cgcgtgagag ggttgcctgg
     4381 gttacttgaa tgtcgttctt agcaagaagg gcttgcaact cagtctgtgt atgaatcttg
     4441 ttttttgtga taagagcgcg tataagttgg tggcggtgtt ctgatttatt cataataatg
     4501 taactccttt tagcaaggta aggtaagcat ggactgagcg aggtcgacag tcaagtggta
     4561 gtctgtattg tcacggatgg tgatttcaaa gtcagtagta tagaggacta aacggagagt
     4621 gtctccttct tttagcttgt aaatagttgg ctgcagttca aattgaacat ccatccattc
     4681 atctgcagta atatcctcta ctaacagtaa atcatttcta ttttgtaaat taaggtaacc
     4741 ttttgtcacg actcgttgtg cctctggtct aaatggcaat tcacagagat tttccaacat
     4801 gtgatagcga ccgttgtcaa tggttctagc acttaaaata gctggataag gttgtaggta
     4861 tttcttttgc ccaaattcta gcagttgggc agataagagc cccttgtttg tactggattt
     4921 gatacgaaga ttgagctgag cgcgaccgtt taggtggaga tctttagtca caggaaggtt
     4981 aatagtaatc tgattggctt tcccttgata gagctctgta ttgaaggttt ggtatgtctt
     5041 accatagcgc tcaaaatcct tatctgggta ctggttttga atagcttgct cttcttgacc
     5101 aagtgagaag gtttcacagt tttcttgccc accgaagtta tcaagtgata accaagtctg
     5161 tggagctgta ttgtcctgcc agataacagt aggaagttga aagtctgttt cctgtcctag
     5221 taatttcttg gtcaataagg catttatgga ctcacggaag tcaattgatt gccaattgtt
     5281 catgtaaaca tgggcaccat tatggaaaaa gagatgcttg tttatatgag taggaagagc
     5341 atggaacatc tggtaaacat gaagtggttt gacattccaa tcctgagaac catgagtaaa
     5401 gacaacctct gcctttactt tatgggcatt gagcagataa ttgcggtcat gccaaaactg
     5461 attgtagtcc ccagtttttc ggtctagctg agctttcact ttttctaagt cagcttggtg
     5521 agcttcattg ccacggatat agtcgccagc taagagatta cgagaatagg ttaactcagc
     5581 aagggagtca aagtcctcac ctggataacc acctgggcta gtcaccagac cgttttcacg
     5641 gtagtagttg taccatgatg aaattcctgc ctcggcaatg ataacttcta aaccatcgac
     5701 tcctgtagtc gcaagaccat tggacatggt acctagatag gaaagtcctg ttgtagcaac
     5761 ttttccgttt gaccaatcag ccttgacttg acgctggcgc gtgtgatcag taaaggcacg
     5821 gcaacggccg ttaagccaat cgatgacatt tttataagcc tcgatttgct ggtagtctcc
     5881 attagtcatg aaacctgtcg agtctttggt accaacacct gagacataga gattggcaaa
     5941 gcctctcgga aggaagtagt cgtttagtgt atagctagag ttgatgtgag ttagcttttc
     6001 ctcagcctct gctataagct cagctttacc ttggggttgg acgagattta gttgaggttt
     6061 ctctagctca atcttgtgag gaagcttaac ctcaagctcg ccctccatct tgtagagagc
     6121 cttgtcacta gccttgtcat tggttccctg atgataaggg ctggctgtca tgatggcagg
     6181 gatttttcca tcaaaacgag ggcgaataat gctaaccttt actaggtctg atagcccttt
     6241 ttggtcagta tcgacacgag actcaacgta aacgacttca cgaatgacat cctggttaga
     6301 aaaagtagcc aaactcttgc cgttaaagta gtggtagtca ttatcctccg gaataagacc
     6361 atcactaaca agttggtcga taagagtatt tccttttttg gtgcgagtat tgagtaactg
     6421 atagagattt tcaatcaagt caccatatat aatgggaaat ccagtttctt tacgaaaaac
     6481 gtcactatct tcgaagtcaa ccaaataaga aaagcctaaa agttgaaaag caacagtata
     6541 aaaaatatct gctgtcagtt catcttctga ttgaaaaaat gtcagcaggt ctgttttttt
     6601 atcagctgct aggatagaaa gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg
     6661 taaaaaggtt tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa agccacattt
     6721 ttttagttca gataagacat tttcttttgg aaaattgata taactatatt gattaaaacg
     6781 catagaacct ccatatagaa tgacagttaa ggttattata tcaaaaaaaa agcagaaagg
     6841 gaattgttaa cttcaaaagg aaataatcca ataaaaatga ataaagtact aaattcaata
     6901 tagagaacag agtaacaata agaataaata gatagggtat aaaagttcta ggagatttat
     6961 attatatgct ttctattttt atatacaata tagtataaat ataaaaatga tgacaaaaat
     7021 acaaatgaat agaaaataaa ttagtaagct gatgaaattt ttctcaagag aagccattta
     7081 taggtgaaaa tggtataata tagtgagaag gatagaggag aagtgtaaat tgatcgcaca
     7141 actagataca aaaacagtct atagttttat ggaaagcgtc atttcgatcg aaaagtatgt
     7201 gagagcagct aaagaatacg gctacactca tttggctatg atggatattg acaatcttta
     7261 tggcgctttc gactttctag agattacaaa aaaatacggc attcatcctt tgctagggct
     7321 tgaaatgaca gtgtttgtag atgatcagga agtgaatttg cgctttttag ctctatctag
     7381 tgtgggctat cagcagttga tgaagctttc gacagccaag atgcaggggg agaaaacttg
     7441 gtcagtcctg tcccagtacc tggaggatat cgcggtcatt gtgccttatt ttgatagagt
     7501 tgagtcgtta gaactaggct gtgattacta tataggggtt tatccagaaa cactagcaag
     7561 cgaatttcat catcctatct tacctcttta tcgggtcaac gcttttgaaa gcagggatag
     7621 agaagttctt caagttttaa cagcgattaa agaaaatcta ccgctcagag aagttccctt
     7681 gcgttcgaga caagatgtct ttatatcagc aagttcttta gagaaactat tccaagagtg
     7741 ttttccgcaa gctttggaca atttagaaaa gcttatttca ggcatttctt acgacttgga
     7801 tactagtctg aaactgcctc gttttaatcc agctagacca gcagtagagg agttgagaga
     7861 gcgtgctgaa ctggggcttg ttcagaaggg gttgactagt aaagaatatc aagatagact
     7921 agaccaagaa ttgtctgtta ttcatgatat gggctttgat gattatttct tggttgtttg
     7981 ggatttgttg cgttttggac gatcgaatgg ctattatatg ggaatgggaa ggggttctgc
     8041 agtaggcagt ttggtttctt atgccttaga catcacgggg attgacccag tagagaaaaa
     8101 tctgattttt gaacgctttc ttaatcgtga acgctatacc atgcctgata ttgatattga
     8161 tatcccagat atttatcgtc cagattttat cagatatgtt ggtaataaat atggtagtaa
     8221 acatgcggca caaatcgtta ctttttcaac ctttggagcc aagcaagctc ttcgagatgt
     8281 cttgaaacgc tttggtgtgc cagagtatga attatctgca attactaaga aaatcagttt
     8341 tcgtgacaat cttaagtcgg cctatgaggg caatctccag tttcgtcagc aaatcaatag
     8401 taagttagaa taccaaaaag cttttgagat tgcttgcaag atagagggct atccaagaca
     8461 aacctctgtc catgcggctg gtgttgtaat tagtgaccaa gatttaacca actatattcc
     8521 tctaaagtat ggtgatgaaa ttccactgac tcagtatgat gctcatggag ttgaggctag
     8581 cggacttttg aagatggact ttctgggact acgaaatttg acctttgttc agaagatgca
     8641 agagttgctt gctgaaatag aaggtattca ccttaaaatt gaagaaatag atttggaaga
     8701 caaagaaacg ttagctttat ttgcctctgg aaatacaaaa ggtatctttc aatttgagca
     8761 accaggtgcc attcgtctgc ttaagcgtgt gcaaccagtc tgttttgaag atgtcgtcgc
     8821 gactacttct ctaaatcgac cgggtgctag tgactatatc aataattttg tggcaagaaa
     8881 gcatgggcag gaagaagtga ctgttctgga tccagtactg gaggatattt tggctccaac
     8941 ctacggcata atgctctatc aggagcaggt tatgcaggtt gcccagcgat ttgccggatt
     9001 tagtcttggg aaagccgata ttttgcgtcg ggctatgggg aaaaaggatg cctctgccat
     9061 gcatgagatg agggcttcct ttattcaagg ttcattagaa gctggtcata ctgtggaaaa
     9121 agcagagcag gtctttgatg ttatggagaa gtttgcaggt tatggtttta acaggtcaca
     9181 cgcctatgcc tactcagcct tggccttcca gttggcttat tttaaaacac attatccagc
     9241 cattttttat caggtcatgt taaattcttc caacagtgat tacttaatag atgcacttga
     9301 agcaggtttt gaagtagcct ctctatccat caacaccatt ccctatcacg ataaaattgc
     9361 caacaaggcc atctatctag gtttgaaatc cattaaagga gtcagtaatg atttagctct
     9421 ctggattatt gaaaatagac cttattctaa cattgaagat tttatagcta aattacctga
     9481 gaattatctg aaacttcctc tgctagaacc tttggtaaaa gttggtcttt tcgattcatt
     9541 tgaaaaaaat cgtcaaaaag tatttaataa tttagctaat ctatttgaat ttgtgaaaga
     9601 gttgggaagt ttgtttggag atgctattta tagttggcag gaatcggaag attggacgga
     9661 acaagaaaaa ttttatatgg aacaagagct tttagggata ggtgtcagca aacatccact
     9721 acaagctatt gcaagtaagg ctatttaccc gattacccca atcggaaatt tgtcagaaaa
     9781 tagctatgct attatcttgg ttgaagttca gaaaataaaa gtgattcgta ccaaaaaggg
     9841 tgaaaatatg gccttcttac aggcagatga tagtaagaaa aaattggatg tcactctctt
     9901 ttcagactta tatcgtcagg ttggacagga aataaaagag ggagccttct actatgtaaa
     9961 aggaaaaata caatcacgtg atggccgtct gcaaatgatt gcacaagaaa taagagaagc
    10021 agttgctgaa cgcttttgga tacaggtgaa aaatcatgaa tcggatcaag aaatttcacg
    10081 cattttagaa caatttaaag gcccaatccc agtcatcatc cggtatgaag aggaacagaa
    10141 aaccatcgtt tctccccatc attttgtagc taaatccaat gaattagagg agaaattgaa
    10201 tgaaatcgtt atgaaaacga tttatcgcta aaaatacgga aaatagaaga attttaaaat
    10261 caaatgtgat ataatcaaga agaatgttaa aagaaaaagg agcataaacc aatatgaaac
    10321 gtattgctgt tttgactagt ggcggagacg cacctggtat gaacgctgcc atccgtgcag
    10381 ttgttcgtca agcaatttca gaaggaatgg aagtgtttgg tatctatgac ggatatgctg
    10441 gtatggttgc cggtgaaatt catcccctag atgcagcttc agtaggggac atcatttctc
    10501 gtggtggtac ttttcttcac tcagctcgtt atccagagtt cgctcaactt gaagggcaac
    10561 ttaaagggat tgagcaattg aaaaaacacg gaattgaagg tgtagttgtt atcggtggtg
    10621 acggatctta ccacggcgct atgcgtttga ctgaacatgg cttcccagct attggtcttc
    10681 caggtacaat cgataacgat atcgttggta ctgactttac aatcggtttt gacacagcgg
    10741 ttactactgc catggacgct atcgataaga ttcgtgatac atcatcaagt caccgtcgta
    10801 cttttgtaat cgaagttatg ggacgtaacg ctggtgatat cgctctttgg gctggtattg
    10861 caactggtgc tgatgaaatc atcatccctg aagcaggctt caagatggaa gatatcgtag
    10921 caagcatcaa agctggttat gaatgtggta aaaaacacaa tatcatcgtc ttagctgaag
    10981 gtgtgatgtc agcggctgaa tttggtcaaa aacttaaaga agctggagat acaagcgacc
    11041 ttcgtgtaac agaacttgga catattcaac gtggtggttc tccaactgcg cgtgaccgtg
    11101 ttttggcgtc acgtatgggt gcacatgctg ttaaacttct taaagaaggt atcggtggtg
    11161 ttgcggttgg tattcgtaac gaaaaaatgg ttgaaaatcc aattcttggt actgcagaag
    11221 aaggggcatt gtttagcctt actgcagaag gtaagattgt ggttaacaac ccgcacaaag
    11281 ccgatattga gctatctagc ttgaataaga gcttgtcata atttacaatt tagttaataa
    11341 gaccaaaaag gtcatatata taaaggagtc acaaaaatca tgaacaaacg tgtaaagatc
    11401 gttgcaactt tgggtcctgc ggtagaaatc cgtggtggta aaaaattcgg tgaggatgga
    11461 tactggggtg aaaaacttga tgttgaagct tcagctaaaa acattgctaa attgattgaa
    11521 gctggtgcta acacattccg attcaacttc tcacacggcg accaccaaga acaaggtgag
    11581 cgtatggcaa ctgttaaact tgcggaaaaa attgcaggta aaaaagttgg tttccttctt
    11641 gatacaaaag gacctgaaat ccgtacagaa ttgttcgaag gtgaagctaa agaatattca
    11701 tacaaaactg gtgaaaaaat tcgtgttgca actaaacaag gaatcaaatc aactcgtgaa
    11761 gtgattgcgt tgaacgttgc tggtgctctt gatatctatg atgatgttga agttggtcgt
    11821 caagttttgg ttgacgatgg taaacttggt cttcgtgtgg ttgctaaaga tgatgcaact
    11881 cgtgaatttg aagttgaagt tgaaaacgat ggtatcatcg ctaaacaaaa aggtgtgaac
    11941 atccctaaca ctaaaattcc tttcccagct cttgctgaac gcgataacga cgatatccgt
    12001 ttcggtcttg aacaaggtat caacttcatc gcaatttcat tcgtacgtac tgcaaaagat
    12061 gtgaacgaag ttcgtgcaat ctgtgaagaa actggaaacg gacatgttca attgttcgct
    12121 aaaatcgaaa accaacaagg tatcgataac ttagacgaaa tcatcgaagc agctgatggt
    12181 attatgattg ctcgtggtga tatgggtatc gaagtaccgt tcgaaatggt tccagtttat
    12241 caaaaaatga ttatcaagaa agtcaatgct gcaggtaaag ttgttatcac tgcaacaaac
    12301 atgcttgaaa caatgactga aaaaccacgt gcaactcgtt cagaagtatc agacgtattc
    12361 aacgctgtta tcgacggaac tgacgctaca atgctgtcag gcgagtctgc aaacggtaaa
    12421 tacccactcg agtcagtaac tacaatggct acaatcgaca agaacgctca agctcttctt
    12481 aatgaatacg gacgtcttga ttcagattca tttgagcgta actctaagac agaagtaatg
    12541 gcttctgctg ttaaagatgc tactagctca atggatatca aattggttgt aactcttact
    12601 aagacaggtc atactgcacg tttgatttct aaataccgtc caaatgctga catcttagca
    12661 ttgacatttg acgaattgac agaacgtggc ttgatgttga actggggtgt tatcccaatg
    12721 ttgacagatg ctccatcttc aactgacgat atgttcgaaa tcgctgaacg taaagcggta
    12781 gaagcaggtc tcgttgagtc aggcgatgat atcgttatcg ttgctggtgt gccagtagga
    12841 gaagctgttc gcacaaacac aatgcgtatc cgcacagtac gttaagaaaa atataaaaac
    12901 ctatcatatc cagctttcta gcctgtatga tgggctcttt ttttgtccag ggggcaaaaa
    12961 ggggggcaaa actttcataa tggattgata ctcttcgaaa atcaaattca aaccacgtca
    13021 gcgttgcctt accgtactca agtacagctt gcggctagct tcctagtttg ctctttgatt
    13081 ttcattgagt atgaaataag atatgcacaa attgattagg aaattcaaat gaatttctac
    13141 aaatgtttta gcaatcgtaa tgtacttgtc tagattcgat ctgatatatt ttcgatttaa
    13201 tgatatggta tttaaaacct ccaaagtagc ttgctccatt cttttactta cgtgagtgta
    13261 gatgttattt actgttttag cgtttttgtg ttccactcta accattatag cattcttctc
    13321 agctagtgta ctaaggagtg tgtgcctgaa aatatgggaa ctaaggggct ggtttatcgg
    13381 tttctctagt ttagtatttg ccttttgcaa agtgatgtta aatgcctttc tctaaattta
    13441 catatcacta ttgtttaaca aaatctaatc tattttaggt cacttattct tttttttgaa
    13501 atgtagaatg aactttttca aagtttttcg aatcttttaa aatctgtttg ttttatatcg
    13561 ccattctccc ccttttttaa ttctccctat atagcctgac agctttcccg atggtacgaa
    13621 tatggttgct ttcgtctagg tggatgtcgg ggtattcggg attgagtttt cttgaggcag
    13681 ccttggcgga gtttcttgac atagttagtg ccgtctactt ggaagatgcc gatggtatta
    13741 tagtcaatct gtggggtatt cttgataaat aggtagtcgc tgtttcttat ctttggctcc
    13801 atggacttgc tgacgacata agcgattggg tcgtagtcgt ctgggataat ggaaactcca
    13861 tatctaaatc gttgtcctgc atcgagcggc tacctgcaga gataaactac ctaacacgag
    13921 agtaagtagt ctgtctgtag tcgtccagtc tgatgatttt tacgatactt cgtttatctt
    13981 cgtttttctg ctcatacagt tgcctctagg cataggttag gactttacct tgtctgggtg
    14041 gtgcccgttg gtcgtagata gattggatat cgctaggaga atccttttga actggaggaa
    14101 agagggcatc aatcaagcta ctgaatactt tagctaagtc aaatatagta tttttcttag
    14161 tagacttaac ccttttttca taatttccaa tggtgttttt acttatacct atcttagtac
    14221 ccaattctta ttgagtccaa ccattactag tctatattgt tttatagttg attgagtttg
    14281 gaatagtacg ctgtagctgc taaaacattt ctagaaatta atttgacttt cctaatagag
    14341 ttgttcatat cttatttcaa tctattatgt ttttcacctc taacaatcgc aatctcttct
    14401 ttatccatga atgaaatcgc tttctatttt tgtaagtaaa gcattacacg aaatccacga
    14461 aaatgaaaac ctttgttgtg ttttcgtaaa aaatttgttg acagagcacg aaacgcctac
    14521 tataattaaa tcaagcttaa ggaaataaca aaaaaacaaa tcggagggaa agacgatgaa
    14581 cacattaaat gagaaagtaa tcaatatctg taaagcaata gttaaagaaa ctttaatcca
    14641 agacatttag gagaaagaaa tgaagaaatc aagaaaatta gccacattag gaatctgttc
    14701 agctttgttt ttaggcttgg ctgcttgtca acaacaacat gctacttctg aggggacgaa
    14761 tcaaaggcaa agcagttcag cgaaagttct atggaaagct tcatacacca acctaaacaa
    14821 ccaggtaagt acagaagagg tcaaatctct cttatcagct cacttggatc caaatagtgt
    14881 tgatgcattt tttaatctcg ttaatgacta taataccatt gtcggctcaa ctggcttatc
    14941 aggagatttc acttccttta ctcacaccga atacgatgtt gagaaaatca gtcatctctg
    15001 gaatcaaaag aagggcgatt tcgttgggac caactgccgt atcaatagtt attgtctttt
    15061 gaaaaattca gtcaccattc caaagcttga aaagaatgac cagttgcttt tcctagataa
    15121 tgatgcgatt gataaaggaa aggtctttga ttcacaagat aaggaagagt ttgatattct
    15181 attttcgaga gttccaactg aggcaactac agatgtcaag gttcacgctg aaaagatgga
    15241 aacattcttc tcacaatttc aattcaatga aaaagctcga atgctgtctg tagtcttgca
    15301 cgacaatctg gatggcgagt atctgtttgt aggccacgtt ggggtcttag tacctgctga
    15361 tgacggtttc ttatttgtag agaaattgac tttcgaagag ccctaccaag cgattaaatt
    15421 tgctagtaag gaagattgct acaagtattt aggcaccaag tatgcggatt atacaggcga
    15481 gggactggct aagcctttta tcatggataa tgataagtgg gttaaacttt aatagtgtta
    15541 tgatatgaca gtaatcttag gatttttatt tctatttttg atgagttggt gggttatttc
    15601 aatcgttaat agccagaaac cactcaaaaa tgaaacattt attggctatg tccaaagata
    15661 cgatattgat gggaatggct atcaatcatt accgtgtcct cagaactgag aggagttctt
    15721 gaaatcgtaa caccactttg aacaagagtt acttcaaccc attggctccg acggagtaag
    15781 ttgctttcgt gaacaccaaa atcagccgca atttcttcat aagttcgata ttctcgcaca
    15841 tattgaagag tggccataag aaggtcttct aggcttaatt taggttttcg tccacctttt
    15901 gcgtgtttaa gtcgataagc tgtttttaat acagctaaca tctcttcaaa agtggtgcgc
    15961 tgaacaccaa caagacattt aaatcgtgca tcagttagtt gtttacttgc ttcataattc
    16021 atagaactac tataccatat tttgtttcgc aggaagtcta atgctaaaat cttatcagtg
    16081 agaagatttc ttaaagttta ttctttgtag tttcatctgt attagtacat aatttaactt
    16141 agagaaaata ggaggactaa tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa
    16201 atgattaaga aaaggcttac aatgtctaat attagtgaca aagaaactac tgagaagatt
    16261 ctggcgctag taccacaaga cttgattaag cgaatcccgt tttttgtcag aaaacatgct
    16321 acaacacgta cgattaaacg catttcaatt gaacaccctg aactctacgc tgtagctcaa
    16381 acaagtggtg agattccaga aaaaaaacgc gaagaattgc gtcagattat cacaactatc
    16441 tttgaacaaa agatgaataa gcatagtatt aagtagagaa tgaaaaaata ttttattggc
    16501 ggtttgggaa ataatgccta tcatagcaag gattttcttc aagaactaga ttcgcaggtc
    16561 tattttctaa atccatatga aaagcatctt cgagatgaaa cagaattgaa atcatggttt
    16621 aaaaatgaga ttgtagagga agaatctatc tgtctgatag gccattctct tggaggagat
    16681 ttagctcgtt atctcacatc ggaatttgaa gaagtaaaga aactgattct tttggatggt
    16741 gactatctag atttagataa gattttacct ttggatacag agttagagga aactaaaaat
    16801 tatatcaaat ctcaaattgt tttggactta gctcttctta tttctaaaga aaaatctgaa
    16861 gcaaagcatt ggtcagaaaa tatggagaaa gctgtaagac agtcttatca ctggaatgtt
    16921 gagtataata gatatgagtt ggctataaat tatgaaaata tagaagcgat actccgccta
    16981 cggagaaaaa tacaagcttt taagagagaa gtgggagata ccttgtttat tagtcctcgc
    17041 tatcctaatg aagctacatg gagagaggaa gccctaaaag aattgccaga ctattttgat
    17101 actatttttt tagaaaactt tggccatgag ctttatactc aagcacctaa agaaatcgct
    17161 agtctaatga atgagtggct cgcttatttt ctatgagcta cttgtattgc tggaaatggt
    17221 ataagatgaa aatctacaag tcactaattt tcatataaca aaaaaacgaa caggctcttt
    17281 ttctgttatt cagaagagaa aaagagcctg ttcgttttta tcttgatttt cttaaaaagc
    17341 tctatataca tagggatttt ctggtttatc gactttggta aagctgtcga tttccaaggt
    17401 tggaaggttt tgtttgagtt ctttatggta gtgattgacc agttttcctt tggctgaaat
    17461 ccaagtgttg ttttcatact gccgggtatt gcccttggtc agcaatccat agacaccaga
    17521 atctgcgata cagtggataa tgccgaatcg gaacagaaat tgactattgg catgactggg
    17581 gtcgttatag acaaagcctg taaactggat tgtcttgccc tcaaattcat ctggatagtc
    17641 gtagatagcc tccatgactt ccatatagtt ttcattagtg atctgaatac tatcttggga
    17701 taagtattta tccgccgccg ttcgcatttc cttttcatag gctgattttg aaaaataaga
    17761 actggtatct ggtttcaaat attggcttgt cgtcccttcg cttgtctgaa tggctagatc
    17821 cgttccttcc gataggggga aatgataacc tttagcagaa acggtctgag aatccaagct
    17881 aacagttggg aaagttaagc cgatgacaag tggaatagcc agaagagaaa tactcgtcat
    17941 cttggccaat cggctgttca gatgactgtg ggttttgact tgcttcatcc agatatacaa
    18001 ttgaacaatt gccaagataa aggaaagcac catggagata taggccagat aggaatagtg
    18061 catgttgatg tactggttta gtttgcccga cagatggagg taaatagtca gttcaaaata
    18121 gccagctaaa actaaaaatc gaatcatagg atgactccaa tcaagagaga ataggctaaa
    18181 acgaccagag tcacaatcgt aatgaagtga ctaataaatc gtgctttcaa gtaatttttc
    18241 atcatgagaa tatttttgat atccagcatt gggccaatga cgagaaaggc cagaactggt
    18301 gccaaaccga aactcgagag tagagaagca cctataaagg catccgcctc actacagaga
    18361 gaaagaagaa aggccaaaag catcaagagc aggatggcaa aaagaggagt tgcactgata
    18421 gaggtcagaa tccgagtcgg aacgtagacc tgtattatag aagcaaagag gcagccaaat
    18481 accaaataac gccccgtatc aaaaaattca tcaatggcct gcacaaagac ttgaaaaact
    18541 ttttttgcag aactcaagta agaaaaatca tgctcatgac aagccagacg attttctttc
    18601 tgaatcggtt cttgccagaa aaatcctaga aaaattccta gtattacagc cacaagaatg
    18661 gaacccagag ctcgtaatag ggcgacatgg aaggagttgc caaaggcaga ataggtcgca
    18721 aaaagaacaa tgggattgat aacaggtgct gtcacaagaa aaggaacggc tgtgtaactg
    18781 ggaacctttt tttccagaaa acgattgatg atggggacga ttccacattc acaagaaggg
    18841 aaaagcatac cgacaaaggt cccaaaaaag attctccccc aacgatttcg agggagaaaa
    18901 tgataaacct tgtcaggtgt gatataaact tcaatcagcc ctgagacaat gcttcctatt
    18961 agaacgaagg gaagggcttc aatgataatg gagagaaaaa tggctccagt ttgaagtaca
    19021 ctagatggga gttgttgaaa agcagtcatc tagtttttct tttctgcttt ggcttttttc
    19081 tctttgtcat cagggaaagt aacttgtttc aaatcaggga gtttagcaaa ctcttcaaac
    19141 atcttgtcca agtcgtctcg ttttgtaaat ttgacagcca taggtgtacc tcgattctaa
    19201 attctacctc tattatacta ttattttgcg gaaaagggaa aagttaaaat ttcaaattga
    19261 gtagctaaga gataatatag cttgatatta cctg
//
LOCUS       DAVDWM010000041        19007 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVDWM010000041 DAVDWM010000000
VERSION     DAVDWM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.041"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            32..496
                     /locus_tag="VUW49_001635"
     CDS             32..496
                     /locus_tag="VUW49_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET7981526.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            654..1079
                     /gene="lacA"
                     /locus_tag="VUW49_001636"
     CDS             654..1079
                     /gene="lacA"
                     /locus_tag="VUW49_001636"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET7981527.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1110..1625
                     /gene="lacB"
                     /locus_tag="VUW49_001637"
     CDS             1110..1625
                     /gene="lacB"
                     /locus_tag="VUW49_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET7981528.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1636..2565
                     /locus_tag="VUW49_001638"
     CDS             1636..2565
                     /locus_tag="VUW49_001638"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET7981529.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2567..3547
                     /gene="lacD"
                     /locus_tag="VUW49_001639"
     CDS             2567..3547
                     /gene="lacD"
                     /locus_tag="VUW49_001639"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET7981530.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3781..4617
                     /locus_tag="VUW49_001640"
     CDS             3781..4617
                     /locus_tag="VUW49_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET7981531.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIIIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLKGYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4653..4970
                     /locus_tag="VUW49_001641"
     CDS             4653..4970
                     /locus_tag="VUW49_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7981532.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            4970..6661
                     /locus_tag="VUW49_001642"
     CDS             4970..6661
                     /locus_tag="VUW49_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET7981533.1"
                     /translation="MNKLIAFIEKGKPFFDKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAKYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            6876..8282
                     /gene="lacG"
                     /locus_tag="VUW49_001643"
     CDS             6876..8282
                     /gene="lacG"
                     /locus_tag="VUW49_001643"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET7981534.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8325..8576
                     /locus_tag="VUW49_001644"
     CDS             8325..8576
                     /locus_tag="VUW49_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET7981535.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDADLEEFKNLFLNFINPEEWDTISFDSDFMPFQ"
     gene            complement(8861..9622)
                     /locus_tag="VUW49_001645"
     CDS             complement(8861..9622)
                     /locus_tag="VUW49_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7981536.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9804..10766)
                     /gene="nrdF"
                     /locus_tag="VUW49_001646"
     CDS             complement(9804..10766)
                     /gene="nrdF"
                     /locus_tag="VUW49_001646"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET7981537.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10954..13113)
                     /gene="nrdE"
                     /locus_tag="VUW49_001647"
     CDS             complement(10954..13113)
                     /gene="nrdE"
                     /locus_tag="VUW49_001647"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET7981538.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13194..13412)
                     /gene="nrdH"
                     /locus_tag="VUW49_001648"
     CDS             complement(13194..13412)
                     /gene="nrdH"
                     /locus_tag="VUW49_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET7981539.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13763..14026
                     /locus_tag="VUW49_001649"
     CDS             13763..14026
                     /locus_tag="VUW49_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET7981540.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADAGDAIAAISETMEKEGLA"
     gene            14032..15765
                     /gene="ptsP"
                     /locus_tag="VUW49_001650"
     CDS             14032..15765
                     /gene="ptsP"
                     /locus_tag="VUW49_001650"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7981541.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            16019..18469
                     /gene="phtA"
                     /locus_tag="VUW49_001651"
     CDS             16019..18469
                     /gene="phtA"
                     /locus_tag="VUW49_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET7981542.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            18627..>19007
                     /locus_tag="VUW49_001652"
     CDS             18627..>19007
                     /locus_tag="VUW49_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET7981543.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYV"
BASE COUNT         6001 a         3462 c         3935 g         5609 t
ORIGIN      
        1 taaagaaact agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtcc
       61 tctacttgat gatgaagtct atgaagatta ttatctagag tttgagaaag aagagatttg
      121 ctctgttcca cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg
      181 gctagaggga caaaaagaaa tagatcttag ttatgatctt ttcagcacag atgcagtgac
      241 tttggatgaa ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataagggatt
      301 gaaagtgcat tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc
      361 tttcattacc tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt
      421 agaagataag aagaatgttt gtctcttaga agccaatcag gttgaagaat tagggtttga
      481 gatagaagtt ttataaaaaa agtaaaaaat aaataacaaa aacaaacata aactgttgac
      541 ttcttcaaac aatagtagta tattatgttt gtgaggacta attgactgtt ttttaaagac
      601 agccaataca ctgctggaat ccagcataga aaaaataaaa aggagtatac aatatgtcta
      661 ttgttatcgg tgcagatgct gcaggtttga gattgaaaga agttgtgaaa gacttcttag
      721 aaaaagaaaa cttccacctt gtggatgtta cagctgaagg tcaagacttt gttgatgtga
      781 ctctcgctgt tgctgcagaa gtaaacaaag aagaacaaaa ccttggtatc gtgattgatg
      841 cttatggagc tggtccattt atggttgcaa ctaagatcaa aggaatggtt gctgcagaag
      901 tatctgacga acgttcagct tatatgactc gtggccacaa caactcacgt atgatcacta
      961 tgggtgcaca acttgttggt gatgaattgg ctaaaaacat cgctaaagga tttgttaacg
     1021 gtaaatacga tggtggacgt catcaaatcc gggtcgacat gttgaacaaa atgggctaat
     1081 tagattacag taagaaaggt aagttaaaaa tgagaattgc aattggatgc gaccacatcg
     1141 taacagatga aaaaatggcg gtttcagaat ttttgaaatc aaaaggatat gaagtcattg
     1201 actttggtac atatgaccat acacggactc actacccaat ctttggtaaa aaagtagggg
     1261 aagctgtaac tagcggccaa gctgatcttg gggtatgtat ctgtggtact ggtgttggta
     1321 tcaacaacgc tgtaaataaa gttccaggtg ttcgttctgc cttggttcgt gatatgacaa
     1381 cagcccttta tgctaaagaa caattgaacg ctaacgttat cggctttggt ggtaagatta
     1441 ctggtgaatt gcttatgtgt gatatcatcg aagctttcat tcatgctgaa tacaaaccat
     1501 ctgaagaaaa caagaaattg attgcgaaaa ttgaacacct tgaaagtcac aatgctcaac
     1561 aaacagacgc aaacttcttt acagaattcc ttgaaaaatg ggatcgcgga gaataccacg
     1621 actaagaggt gacctatgat tttaacagtc acaatgaacc catccattga tatttcctat
     1681 cccttggatg agttgaagat tgatactgtc aatcgtgtgg tggatgtaac caaaacggct
     1741 ggtggtaagg gactcaatgt tacccgagta ctttcagaat ttggcgattc tgttcttgct
     1801 actggtttag tgggtggcaa acttggtgag tttttggttg aacatatcga taatcaagta
     1861 aagaaagatt tcttctcaat tcagggagaa actcgtaact gtatcgctat tctccacgga
     1921 gacaaccaaa cagaagttct tgaaaaaggt cctgaagtat tggaacagga aggtcaagac
     1981 tttttggaac atttcaaaaa actcttggag tcagttgaag tagtggccat ctcaggtagt
     2041 ctgccagctg gccttccagt tgattactat gcgagcttgg tagaacttgc taatcaagct
     2101 ggcaagcctg tagtcttgga ctgctcaggt gcagcacttc aggctgttct tgaatcaccc
     2161 cataaaccaa cagtcatcaa accaaataat gaagaattgt ctcagcttct tggaagagaa
     2221 gtttctgagg atttggatga attaaaagaa gtacttcaag aacctttgtt tgcagggatt
     2281 gaatggatta tcgtttcact tggtgccaac ggtacttttg ccaaacatgg tgacactttc
     2341 tacaaggtag atattcctag aattcaggtg gtaaatcctg ttggatctgg agactctact
     2401 gtggcaggaa tttcttcagg acttcttcac aaagaatcgg atgcagaatt actcatcaag
     2461 gcaaatgtcc ttggtatgct caatgctcaa gaaaaaatga ctggtcatgt caacatggcc
     2521 aactatcaag ctctatatga tcaattaata gtaaaagagg tataaaatgg ctttaacaga
     2581 acaaaaacgt gtacgcttag aaaaactttc tgatgaaaat ggtatcatct cagctcttgc
     2641 atttgaccaa cgtggtgctt tgaaacgcct catggctcaa caccaaacag aagaaccaac
     2701 tgtggcccaa atggaagaac ttaaagtctt ggtagcagat gaattgacta aatacgcttc
     2761 atctatgctt cttgaccctg agtatggact tccagcaact aaagctcttg atgaaaaagc
     2821 tggtcttctc cttgcttatg aaaaaacagg ttatgacaca acaagcacaa aacgcttgcc
     2881 agactgcttg gatgtttggt ctgcaaaacg tattaaagaa gaaggtgcag atgcagttaa
     2941 attcttgctt tactatgatg tagatagctc agacgaactc aatcaagaaa aacaagccta
     3001 catcgaacgc atcggttctg agtgtgtggc tgaagatatc ccattcttcc ttgaaatcct
     3061 tgcttacgat gaaaaaattg cggatgcagg ttctgtagaa tacgcgaaag taaaaccaca
     3121 caaagttatc ggcgctatga aagtcttttc agacccacgc tttaacattg atgtcttgaa
     3181 agttgaagtt cctgttaaca ttaaatatgt tgaaggcttc gctgaaggtg aagtagttta
     3241 tacacgtgaa gaagcagcag ccttcttcaa agcgcaagat gaagcaacga acttgccata
     3301 catctacttg agtgctggtg tatcagctaa actcttccaa gatactcttg tatttgctca
     3361 tgaatcaggt gcaaacttta acggagttct ttgtggccgt gctacatggg cagggtcagt
     3421 tgaagcttac atcaaagatg gtgaagcagc agctcgcgaa tggcttcgca caactggatt
     3481 tgaaaacatt gatgagctca ataaagttct tcaaacaaca gcgacttcat ggaaagaacg
     3541 tgtgtaagaa agtcctccta gtttaggaac atgaatctaa aaaaatttaa aaaaagttgt
     3601 atgtaaaggt ttacaaaaca accgacttgt gctatactta aatcacaagt taatacaagg
     3661 tgagtgttac taagtaatat taggcatgat cacaggtgaa ttagaaatca gctgattttc
     3721 tagttcattt gtggtcattt tttgtactca tataccttta agatataaaa ggaggttgac
     3781 atgtatcgaa ttctaaatcc aatgaatcac aatgtctcgc ttgtcagaaa tgataaggga
     3841 gaagaggtga ttataattgg taaggggatt gcattcggaa aaaggaaggg ggatttgatt
     3901 gctgaaaatc aggttgagaa aatctttcgg atgaagaccg aagagtccag agaaaacttt
     3961 atggctcttc tcaaagatgt tccgcttgat tttatcacag tgacctatga aatcattgat
     4021 aagctatcaa agaaatatca ttatccgatt caagagtatc tctatgtaac cttgacagat
     4081 catatttact gttcttatca agctctaact caaggaaggt acaaggatag taatctgccg
     4141 gatatttccg ctaagtatcc tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac
     4201 cgtcagaagc tagcagatca ttttcctgag gacgagatta ttcggattgc ttatcatttc
     4261 atcaatgccg aaggtgaaaa tgaagtggaa cttgtggagt cgattgataa gaggaaagaa
     4321 attctcagga atgttgaaga agtgctaaag ggttatgcaa ttcaacgaac caaaaaaaat
     4381 aatcatttct atgatcgctt tatgattcat ttgaattatt tcttggatta tttagacaga
     4441 tctagagatg ataaccaatc acttctggat atggaagatc atattaaaca atcctatcca
     4501 aaagccttcg agattggttc caagatctat gatgtgatta cgcaacatac gggtcttgat
     4561 ttgtataaaa gtgaacgagt ttatctagtt ctacatatcc aacgtttatt gtcataaaaa
     4621 tttatttaaa actatataag gagaattcta tcatgaatag agaagaagta acattgttag
     4681 gttttgaaat cgtagcctat gctggcgatg ctcgttcaaa actattggaa gccttgaagg
     4741 ctgctgaagc tggtgatttt gaaaaagcgg acgctctggt agaggaagct ggtagctgta
     4801 ttgcagaggc tcaccacgcg caaacaagtc tattgactaa ggaagcttct ggtgaggact
     4861 tagcgtatag tgtgaccatg atgcatggcc aagaccactt gatgacaact atcttgttaa
     4921 aggatttgat gcaccatttg atcgaactct ataagagagg agttaaataa tgaacaaact
     4981 aattgcattt atcgagaaag gaaagccttt ctttgataaa ctatctcgta atatctatct
     5041 tcgtgctatt cgtgatggtt tcattgcagg tatgcctgtt attctcttct caagtatctt
     5101 tatcttgatt gcctttgtac caaactcatg gggctttaaa tggtctgatg aagttgtagc
     5161 ctttctgatg aaaccttata gctattctat gggtattctg gctctcttgg tagctggtac
     5221 aacagctaag tcattgaccg actcagtaaa ccgtagcatg gagaaaacca atcaaatcaa
     5281 ctatatgtca acgctgttag cagcgattgt tggtttgttg atgttggcag ctgatcctat
     5341 cgaaaatggt ctagctactg gattcttggg gacaaaaggt ttgctttcag ccttccttgc
     5401 tgcctttgtt actgtagcca tctataaggt ctgtgttaag aacaacgtca ctattcgtat
     5461 gcctgacgaa gttccaccaa atatctcaca agtctttaaa gatgtgattc cattcactct
     5521 atcagtggtt tctctttatg ctcttgattt actagctcgt cattttgttg gttctagtgt
     5581 ggcagaatca atcggtaaat tcttcgcacc actcttctca gcagcagacg gataccttgg
     5641 tattaccatt atctttggtg cctttgcctt cttctggttt gtgggtattc atggtccatc
     5701 tattgttgaa cctgctatcg cagcgattac ctatgcaaat gctgaagtca acttgaacct
     5761 tctccaacaa ggtatgcacg ctgacaagat tcttacttct ggtacacaaa tgtttatcgt
     5821 taccatgggt ggtactggtg cgacactggt tgttccattc atgttcatgt ggttaacaaa
     5881 atcaaaacgt aaccgtgcaa tcggacgtgc ttcagtagtt cctaccttct tcggtgtaaa
     5941 tgagccaatc ttgtttggtg cacctcttgt tttgaatcca atcttcttca ttccatttat
     6001 ctttgctcca attgcaaacg tatggatttt caaattcttt attgaaactc ttggaatgaa
     6061 ctcattcact gctaatctac catggacaac tccagctcca ctaggtctag ttcttggaac
     6121 taacttccaa gtgctatcat tcattcttgc tgcccttcta atcgtggttg acgttgtcat
     6181 ttactatcca ttccttaagg tctatgatga acaaattctt gaagaagaac gttcaggtaa
     6241 gtctaatgat gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc
     6301 tattcttgaa aaagcaggtg tcgatgcagc acaaaatacc atcactgaag aaacaaatgt
     6361 cctcgttctc tgtgctggtg gaggtacaag tggactcctt gcaaatgctt tgaataaggc
     6421 agcagcaaaa tacaatgtcc ctgtgaaagc agcagcaggt ggctatggtg ctcaccgtga
     6481 aatgttgcca gagtttgatc ttgttatcct tgcccctcaa gttgcttcaa actttgaaga
     6541 tatgaaggca gaaacagata agctcggtat taaacttgct aagacagaag gcgctcaata
     6601 catcaaatta actcgtgatg gaaaaggtgc cctagcattc gtacaagagc aattcgatta
     6661 aggctaggaa ctgtgaaaca gtctcccatc gttacggaaa tcgctatggc gaatttccta
     6721 atcgccttat taattcgtcg gtaaaaagat atcgttttta cctcctcatg tcacaattca
     6781 gtgatttggc acaagaagtg agatggagaa gcatggctca ctgactcctc tcctctcact
     6841 tttactttat ttaaatcaag aaataggtga aaaaaatgac aaaaacactt ccaaaagact
     6901 ttattttcgg tggcgcaaca gctgcttatc aagcagaagg tgctacacat actgatggaa
     6961 aaggaccagt tgcttgggat aaatatcttg aggataacta ctggtacact gccgaaccag
     7021 ctagtgattt ttacaatcga tatccagttg acctcaagct agcagaagag tatggtgtca
     7081 atggtattcg aatttctatt gcttggtcac gtattttccc gactggttac ggccaagtaa
     7141 atgctaaagg tgttgagttt tatcataatt tatttgcaga gtgtcacaaa cgtcatgttg
     7201 agccctttgt aactcttcat cactttgaca ctccagaagc tctccactca aatggagact
     7261 tcttaaaccg tgaaaatatc gaacattttg tagactacgc tgccttctgt tttgaagaat
     7321 ttccagaagt aaactattgg acaaccttta atgaaattgg accaatcggt gatggtcaat
     7381 atttggttgg gaaattccct ccaggtatcc agtacgacct tgccaaagtc tttcaatcac
     7441 accacaatat gatggtgtct catgcacgcg cggtaaaatt gtacaaagag aaaggctata
     7501 aaggggaaat tggtgttgtt cacgccctgc ctactaaata tcctctagat cctgaaaatc
     7561 cagcagatgt tcgtgcagct gagttggaag atatcatcca caataaattc atcttagacg
     7621 caacttatct aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat atcttatcag
     7681 tcaatggtgg tagtttggat cttcgtgaag aagattttac agcattagaa gctgcaaaag
     7741 acttgaatga tttcctagga atcaactact atatgagtga ctggatggaa gcctttgatg
     7801 gagaaactga aattatccat aatggtaaag gtgaaaaagg aagctctaag tatcaaatca
     7861 aaggtgttgg tcgtcgtgta gctcctgact atgtaccacg cacggattgg gattggatta
     7921 tctaccctca aggtttgtat gaccaaatca tgcgtgtgaa gaaagattat cctaactaca
     7981 agaagattta catcactgaa aatggtctcg gctataaaga tgagttcgtt gataacactg
     8041 tttacgatga tggtcgtatt gattacgtga aacaacactt ggagatcttg tctgatgcga
     8101 ttgcagatgg agctaatgta aaaggttact tcatttggtc attaatggat gtcttctcat
     8161 ggtcaaacgg ttatgagaaa cgttatggtc tcttctacgt agattttgaa actcaagaac
     8221 gttatcctaa gaaatcagct cactggtaca agaaagtagc ggaaactcag attatagact
     8281 agtagaatta gtcattagat atagaatttt agtaagtcaa aaagatgttc aaagatttta
     8341 tccaatctat ttatgaaaaa gtttatatta taaatttcga aaaatgctct caaataccgt
     8401 gtttgacgag tgaagaattg aaaagtcttg gaaaatggta tgtctcgact ggtaaagaat
     8461 ggatttgtca ttcagatgct gatctggaag aatttaaaaa tctattttta aattttatca
     8521 atcctgaaga atgggatact atctcctttg attcagattt tatgccgttt caataatcgt
     8581 aaccaatttc tcaaaaaagt taaatcttat atttagtact ctgtaaaact cttatctaat
     8641 cacgttgctt atactcaatg aaaatcaaag agcaacttta aactaggaag cgagtcgcag
     8701 atttctcaat gtatagcttt gaggaattgg gcaaaaagtc tttgatatag aaaaaagcat
     8761 agtatcaggt gtttcaacac ctgatactat gctttttatt gtgggaagat ttactttttt
     8821 tcttctgaaa ttgagttgtt acccaggctc tttcagttta ttaaggcttg atgactttaa
     8881 tgtgtttaga tagcttaaaa aggattgaat cacttagttt agaatctgaa acaatagtat
     8941 caagatttga tatattataa aaagtataaa aatcaaactt attgaactta ctatgatctg
     9001 cgagtaaata ttttttatta gaattattta aagcgatgcg ttgagcctct ccctcttcct
     9061 cgctaaaagt agctagagct ccgttttgaa taccattaca gctaacgaaa gctttagaaa
     9121 attggagatt agagagattt tgtagggtca atgtaccaac aaaagcacct gtaatatcgc
     9181 gataatttcc acctattaaa atcaaatctg ttaattttcg ttcgcttaaa atcagaaaaa
     9241 caggtagact gttggttacg acgcggatat tgtcaatagg caactcacgc gcaaaaaact
     9301 ctaatgttgt tcctggtcca atgaaaatag tttctctttc ttctactaga ctgcctgcaa
     9361 aatgggctat ttcttgtttt tctgccgttt ggagggcttg tttttcaata tttgatcgct
     9421 cattagtcaa aagggagttg gttcgaagtt tttcagctcc accatgcaca cgaatcagca
     9481 aatctttatc agctaattcc tgtaaatagc gccttgcagt catatctgaa acggctattt
     9541 cgtccataat ctgtttaact gttatggttc ctttactatt tactatctct aaaattttgg
     9601 ctaatttttc ttgtttgagc atattatcac ctcgtttcct actactatct taccataaac
     9661 aaactcatca ttcaaataca aaaacaacaa aatgaaacaa aaacaaaaat atcgaagttt
     9721 gttttcaaaa ctttcgatat ttttgttggg ttataacttt gatgtttcta gtttactttt
     9781 tgatgattga gagtgatgga gaattagtct aaaccgtagt tatagtcatc gtcttgcatg
     9841 gcttcaactt cgccaagaag gtaaccattt ccgacttgag agaagaagtc atggttggaa
     9901 gttcctgttg aaataccgtt cataacgatt gggttgacat cttcagctga atctgggaaa
     9961 agtggatctt gtcccatgtt catgagagct ttattggcat tgtagcgaag gaaggtttta
    10021 acctcttcag tccaaccaac accgtcataa agactctctg tgtagccttc ttcattttca
    10081 taaagagtat agagtaggtc gtacatccat tctttgagtt tttcttgctc ttcttcaggc
    10141 aattcattga aaccaagttg gaatttgtaa ccaatgtagg ttccgtgaac agactcgtca
    10201 cgaataatca atttaatgat ttctgcaacg ttggctagtt tgttgttacc gagatagtag
    10261 aggggagtga agaaaccaga gtagaagagg aaggtttcga ggaagacgct ggcaactttc
    10321 ttttcaagtg ggctgccgtt taggtagatt tcgttgacaa tctcagcctt cttttgtagg
    10381 taaggattgg tattggtcca ttcgaaaatt tcttcaatct cagccttagt attcaaggta
    10441 gaaaagattg atgagtaaga tttagcgtgg acagattcca taaattggat gttattgaaa
    10501 acagcttcct catgtggtgt acggatgtct gcgcgaaggg cttgaacccc agtttcagat
    10561 tgcatagtgt caagaagggt taaaccacca aaaacttttc cgaccaagtc tttctctttg
    10621 ttagatagct ttctccagtc atccaagtcg tttgataagg gaatacgtgt atcgagccaa
    10681 aattgctccg tcagtttttc ccaagttgat ttgtcgatga catcttcgat ggcattccag
    10741 ttaatggctt tgtagtaagt ttccatttaa aatctctttc tgtgtttagt attgcgaact
    10801 cacaattatt tctactttac cataattcta taggagtatc gcacaaaaag tcggaagccc
    10861 gacttttaaa atgttacata aattatgtta tgacatagta gatttgattt tatcagtgct
    10921 gcttagggaa aaataatgtt tctatgctag aaactaaatc acacagcttt cacattggtt
    10981 ggcgccgact tctccaccgt catctgtaaa ggtacggacg tagtagatag acttgattcc
    11041 cttgttaaag gcatagttac gaaggatgga caagtcacgt gtcgtttgtt tattttccct
    11101 cttccattcg taaaggcctt ttggaatgtc actacgcatg aagagggtga gtgaaagtcc
    11161 ttgatccacg tgttcagtcg cagcagcgta aacatcgatg actttacgca tatccatatc
    11221 gtaggcagaa gtgtagtaag gaatggtttc tgtagacaag ccagcagcag ggtagtagat
    11281 tttaccaatt ttcttctctt ggcgttcttc gatacgttgc gtaatcgggt ggatagaagc
    11341 agaaacgtcg ttgatatagc tgatagaacc atttggcgct acagcaaggc gattttggtg
    11401 gtaaagacca tcttcttgaa ccttgtcgcg aagttcagcc caatcagcaa caccagggat
    11461 aaagacattt ttgaagagtt ctttaacacg gtctgatgtt ggaacaaatt caccagttac
    11521 atacttgtca aagtaacttc cgttagcata gtctgatttt tcaaagttgt ggaaggtaat
    11581 accacgttca cgcgcaatat tgtttgactc taccaaggtc cagtagttca taagcataaa
    11641 gtagatgctt gtaaattcaa cagactcagg tgaaccatat tcaatgagtt gttgggcaag
    11701 gtagctgtgc agtcccatgg caccgagacc aaaggtgtgg gcttggctat ttccatggtc
    11761 aatcgttggt acagctacga tatgtgaact atctgtaacg aaagtaaggg cacgaaccat
    11821 agcacggata gaacgaccaa aatcaggtga agtcatcatg ttaaccacgt tggttgaacc
    11881 caggttacat gaaacatctg ttcccatttg aaggaattct tgagcatcgt tgatcaagct
    11941 tggttcttga acttgaagaa tctcagaaca caagttactc atgataatct ttccatcaac
    12001 aggatttgca cggttagccg tatcgatgtt gactacataa ggatagccag actcttgttg
    12061 caatttagag atttcagttt ccaaatcccg cgccttgatt tttgtcttgc gaatatttgg
    12121 atttgcgacc aattcatcgt atttttcagt aatgtcgatg taattgaatg gcacaccgta
    12181 ttctttttct acagagtaag ggctgaagag gtacatttct tcatttttac gagccaattc
    12241 gtagaattta tcaggtacta caacaccaag tgatagagtc ttgacacgta ctttttcatc
    12301 agcgttttct ttcttagttg aaaggaaagc gatgatatct gggtgaaaga cgttgaggta
    12361 gacaacaccg gcaccttgac gttgccccaa ttggttggag taagagaagc tgtcttcaaa
    12421 aagcttcata acaggaacga cacctgaagc agctccttca tagcctttga taggtgcacc
    12481 agcttcacga aggttgctga gggtaattcc cacaccacca ccaatacgtg aaagttgaag
    12541 agctgagttg atagaacgtc cgatagagtt catatcatcc gtcacttgga ttaggaaaca
    12601 agataccaac tccccacgac gagcacgtcc agcattcaag aaggaaggag tagcaggttg
    12661 gtagcgttgg tggatgattt cattggcaat atcgattgca acagcttcat tcccatcagc
    12721 gaaataaagg gcgttgaaga agacacggtc ttccatattt tcaagatagt attcaccgtc
    12781 attagtcttt aaggcatatt gattgtaaaa tttataagct gccatgaatg acttgaattg
    12841 gaagttttgg tctttgataa attgagccaa ttcttccaag aactctggac ggtatttctt
    12901 gataaaggct gtttcgatgt agttgtgttc aatgaggtaa ttgattttgt ctttgattga
    12961 atcaaaaacc atagtgtttg gaactacatt ttctttaaag aaagcatcca aggcttcctt
    13021 atctttatga agcatgattt gtccattaac aggacggtta atttcgttat taagacggaa
    13081 gtaagtcacg tcttcaagat gttttaatcc cataaaattt cccttatcta attacaaaag
    13141 aaaggcttct aagttagccc taaaagcagt ttcttctgga tgatgtacta agattatgct
    13201 aattgtttca gttttcctgg ttggaaacct gaaaagactt cagttggtgt ttggataaca
    13261 ggagctgcgc taaaaccgag ctctttaact tgatcgacgt actcaggttg ctcatcaaga
    13321 ttgatttcac gataagagac attattactg tccaagaaac gcttggtcat tttacattgg
    13381 acacaattgt ttttagaata aacggttacc attgtgtaac tcctcttcaa aatttaatac
    13441 tatcttagta tatcagaaaa taaaattttg tcaatgattt aaactaaata tagtggtgtt
    13501 cttttgttgt tttcatatat aaacacaata tatagtgttt gtattgtgaa atagcaatga
    13561 ttatcctata aattgttttt gaagaaacta tatcctgtgt tttgttatct aggatagaag
    13621 attttcagca agttatctga aaggaaattg aaggaaaatc cagaaaatgc ttgaaaaaaa
    13681 tcctagaaga tggtataata ctaaattgta agggttatca catataactc aaaaaaagaa
    13741 agaacaaaag gagagtcaaa ctatggcttc taaagatttc cacgtagtgg cagaaacagg
    13801 tattcacgca cgtccagcaa cattgttggt acaaactgct agcaaatttg cttcagatat
    13861 cactcttgag tacaaaggta aatcagttaa ccttaaatca attatgggtg ttatgagtct
    13921 tggtgttggt caaggtgctg acgtaactat ctcagctgaa ggtgcagatg caggtgacgc
    13981 tatcgctgca atctcagaaa caatggaaaa agaaggattg gcataaggga aatgacagaa
    14041 atgcttaaag gaatcgcagc atctgacggt gttgcagttg caaaagcata tctactcgtt
    14101 cagccggatt tgtcatttga gactattaca gtcgaagata caaacgcaga agaagctcgc
    14161 cttgatgccg ctctacaggc atcacaagac gagctttctg ttattcgcga gaaagcagta
    14221 ggtacgctcg gtgaagaagc agctcaagtt tttgatgctc acttaatggt tcttgctgac
    14281 ccagaaatga tcagccaaat caaggaaact atccgtgcga agaaagtgaa tgcagaagca
    14341 ggtctgaaag aagttacaga tatgtttatc actatctttg aaggcatgga agacaaccca
    14401 tacatgcaag aacgcgcagc ggatatccgc gacgtgacaa aacgtgtatt ggcaaacctt
    14461 cttggtaaaa aattgccaaa cccagcttct atcaatgaag aagtgattgt gattgcacat
    14521 gacttgacac catctgatac agctcaattg gacaaaaact ttgtaaaagc ttttgtaacc
    14581 aacatcggtg gacgtacaag ccactcagct atcatggcac gtacacttga aattgctgct
    14641 gtattaggta caaacaacat cactgaaatc gttaaagacg gtgacatcct tgctgttaac
    14701 gggatcactg gagaagtgat tatcaaccca acagatgaac aagcggcaga atttaaagca
    14761 gctggtgaag cctatgcgaa acaaaaagct gaatgggcac ttttgaaaga tgctcaaaca
    14821 gtgactgctg acggtaaaca cttcgagttg gctgctaata tcggtactcc aaaagacgtt
    14881 gaaggtgtta acaacaacgg tgcagaagct gttggacttt accgtacaga gttcttgtac
    14941 atggattctc aagacttccc aactgaagat gagcagtatg aagcatacaa ggctgttctt
    15001 gaaggaatga acggtaaacc tgttgtcgtt cgtacaatgg atatcggtgg agataaggaa
    15061 cttccttact tcgatatgcc tcacgaaatg aacccattcc ttggattccg tgctcttcgt
    15121 atctctatct ctgagactgg agatgctatg ttccgcacac aaatccgtgc tcttcttcgt
    15181 gcgtctgttc acggtcaatt gcgtatcatg ttcccaatgg ttgcgctctt gaaagaattc
    15241 cgtgcagcga aagcagtctt tgatgaagaa aaagcaaacc ttcttgctga aggtgttgca
    15301 gttgcggata acatccaagt tggtatcatg atcgagattc ctgcagcggc tatgcttgca
    15361 gaccaatttg ctaaagaagt tgacttcttc tcaattggta caaacgactt gatccaatat
    15421 acaatggcag cagaccgtat gaacgaacaa gtttcatacc tttaccaacc atacaaccca
    15481 tcaatcctac gcttgattaa caatgtgatc aaagcagctc acgctgaagg taaatgggct
    15541 ggtatgtgtg gtgagatggc tggtgaccaa caagctgttc cacttcttgt cggaatgggc
    15601 ttggatgagt tctctatgtc agcaacatct gtacttcgta cacgcagctt gatgaagaaa
    15661 ctcgacacag ctaagatgga agagtacgca aaccgtgccc ttacagaatg ctcaacaatg
    15721 gaagaagttc ttgaacttca aaaagaatac gttaattttg attaatcgaa aagtccctgc
    15781 aactcagtta cagggatttt tttgatattt taaaaagaat tttcaagaaa atctttctta
    15841 tagaaagtcc aaccttgaaa aagtagtagt cagaacaaaa aatacttaaa tggttcataa
    15901 aattcttgac aagttggaga tttaggagta aactattaac cagttaagta atagagagga
    15961 gtttctgcaa tttagaaatg aattgcaact agaaatatca aatagaaaga gagtttcgat
    16021 gaaaattaat aagaaatacc ttgttggttc tgcggcagct ttgattttaa gtgtttgttc
    16081 ttacgagttg ggactgtatc aagctagaac ggttaaggaa aataatcgtg tttcctatat
    16141 agatggaaaa caagcgacgc aaaaaacgga gaatttgact cctgatgagg ttagcaagcg
    16201 tgaaggaatc aatgctgagc aaatcgtcat caagataaca gaccaaggct atgtcacttc
    16261 acatggcgac cactatcatt attacaatgg taaggttcct tatgatgcga ttttcagtga
    16321 agaattactc atgaaagatc caaactataa gctaaaagat gaggatattg ttaatgaggt
    16381 caagggtgga tatgttatca aggtagatgg aaaatactat gtttacctta aggatgctgc
    16441 ccacgcggat aacgtccgta caaaagagga aatcaatcga caaaaacaag aacatagtca
    16501 acatcgtgaa ggtgggactc caagaaacga tggtgctgtt gccttggcac gttcgcaagg
    16561 acgctatact acagatgatg gttatatctt taatgcttct gatatcatag aggatactgg
    16621 tgatgcttat atcgttcctc atggagatca ttaccattac attcctaaga atgagttatc
    16681 agctagcgag ttggctgctg cagaagcctt cctatctggt cggggaaatc tgtcaaattc
    16741 aagaacctat cgccgacaaa atagcgataa cacttcaaga acaaactggg taccttctgt
    16801 aagcaatcca ggaactacaa atactaacac aagcaacaac agcaacacta acagtcaagc
    16861 aagtcaaagt aatgacattg atagtctctt gaaacagctc tacaaactgc ctttgagtca
    16921 acgacatgta gaatctgatg gccttgtttt cgacccagcg caaatcacaa gtcgaacagc
    16981 tagaggtgtt gcagtgccac acggagatca ttaccacttc atcccttact ctcaaatgtc
    17041 tgaattggaa gaacgaatcg ctcgtattat tccccttcgt tatcgttcaa accattgggt
    17101 accagattca aggccagaac aaccaagtcc acaaccgact ccggaaccta gtccaggccc
    17161 gcaacctgca ccaaatctta aaatagactc aaattcttct ttggttagtc agctggtacg
    17221 aaaagttggg gaaggatatg tattcgaaga aaagggcatc tctcgttatg tctttgcgaa
    17281 agatttacca tctgaaactg ttaaaaatct tgaaagtaag ttatcaaaac aagagagtgt
    17341 ttcacacact ttaactgcta aaaaagaaaa tgttgctcct cgtgaccaag aattttatga
    17401 taaagcatat aatctgttaa ctgaggctca taaagccttg tttgaaaata agggtcgtaa
    17461 ttctgatttc caagccttag acaaattatt agaacgcttg aatgatgaat cgactaataa
    17521 agaaaaattg gtagatgatt tattggcatt cctagcacca attacccatc cagagcgact
    17581 tggcaagcca aattctcaaa ttgagtatac tgaagacgaa gttcgtattg ctcaattagc
    17641 tgataagtat acaacgtcag atggttacat ttttgatgaa catgatataa tcagtgatga
    17701 aggagatgca tatgtaacgc ctcatatggg ccatagtcac tggattggaa aagatagcct
    17761 ttctgataag gaaaaagttg cagctcaagc ctatactaaa gaaaaaggta tcctacctcc
    17821 atctccagac gcagatgtta aagcaaatcc aactggagat agtgcagcag ctatttacaa
    17881 tcgtgtgaaa ggggaaaaac gaattccact cgttcgactt ccatatatgg ttgagcatac
    17941 agttgaggtt aaaaacggta atttgattat tcctcataag gatcattacc ataatattaa
    18001 atttgcttgg tttgatgatc acacatacaa agctccaaat ggctatacct tggaagattt
    18061 gtttgcgacg attaagtact acgtagaaca ccctgacgaa cgtccacatt ctaatgatgg
    18121 atggggcaat gccagtgagc atgtgttagg caagaaagac cacagtgaag atccaaataa
    18181 gaacttcaaa gcggatgaag agccagtaga ggaaacacct gctgagccag aagtccctca
    18241 agtagagact gaaaaagtag aagcccaact caaagaagca gaagttttgc ttgcgaaagt
    18301 aacggattct agtctgaaag ccaatgcaac agaaactcta gctggtttac gaaataactt
    18361 gactcttcaa attatggata acaatagtat catggcagaa gcagaaaaat tacttgcgtt
    18421 gttaaaagga agtaatcctt catctgtaag taaggaaaaa ataaactaat gaaaaatgaa
    18481 agtctcgata aagaggcttt catttttatt atgtatatat gtaaaattct tgacaagcaa
    18541 tattaaaaag agtaaactat taactagtta attaaccggt ttattacttt atagtgaatc
    18601 aaatatactt aagaaaagag gaaagaatga aaatcaataa aaaatatcta gcaggttcgg
    18661 tggcagtcct tgccctaagt gtttgttcct atgaacttgg tcgtcaccaa gctggtcagg
    18721 ttaagaaaga gtctaatcga gttgcttata tagatggtga tcaggctggt caaaaggcag
    18781 aaaatttgac accagatgaa gtcagtaaga gagaggggat caacgccgaa caaatcgtca
    18841 tcaagattac ggatcaaggt tatgtgacct ctcatggaga ccattatcat tactataatg
    18901 gcaaggttcc ttatgatgcc atcatcagtg aagagctcct catgaaagat ccgaattatc
    18961 agttgaagga ttcagacatt gtcaatgaaa tcaagggtgg ttatgtc
//
LOCUS       DAVDWM010000042        18077 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVDWM010000042 DAVDWM010000000
VERSION     DAVDWM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.042"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            32..328
                     /locus_tag="VUW49_001653"
     CDS             32..328
                     /locus_tag="VUW49_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET7981544.1"
                     /translation="MDTPDENGYVADNYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGASIG"
     gene            449..1393
                     /gene="manA"
                     /locus_tag="VUW49_001654"
     CDS             449..1393
                     /gene="manA"
                     /locus_tag="VUW49_001654"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET7981545.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1384..2889)
                     /locus_tag="VUW49_001655"
     CDS             complement(1384..2889)
                     /locus_tag="VUW49_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HET7981546.1"
                     /translation="MIFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFEKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMDFLVSNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKDLSNELRPFLFYG"
     gene            2994..3743
                     /locus_tag="VUW49_001656"
     CDS             2994..3743
                     /locus_tag="VUW49_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7981547.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(3763..4218)
                     /locus_tag="VUW49_001657"
     CDS             complement(3763..4218)
                     /locus_tag="VUW49_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET7981548.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4238..5413)
                     /locus_tag="VUW49_001658"
     CDS             complement(4238..5413)
                     /locus_tag="VUW49_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7981549.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5472..6317)
                     /locus_tag="VUW49_001659"
     CDS             complement(5472..6317)
                     /locus_tag="VUW49_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7981550.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6442..6999
                     /locus_tag="VUW49_001660"
     CDS             6442..6999
                     /locus_tag="VUW49_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7981551.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7018..7485
                     /locus_tag="VUW49_001661"
     CDS             7018..7485
                     /locus_tag="VUW49_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET7981552.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7574..8203
                     /gene="upp"
                     /locus_tag="VUW49_001662"
     CDS             7574..8203
                     /gene="upp"
                     /locus_tag="VUW49_001662"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET7981553.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8377..8967
                     /gene="clpP"
                     /locus_tag="VUW49_001663"
     CDS             8377..8967
                     /gene="clpP"
                     /locus_tag="VUW49_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET7981554.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9046..9294
                     /locus_tag="VUW49_001664"
     CDS             9046..9294
                     /locus_tag="VUW49_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET7981555.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9395..10555
                     /locus_tag="VUW49_001665"
     CDS             9395..10555
                     /locus_tag="VUW49_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7981556.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVSNATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAAFTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATVE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10823..11692
                     /locus_tag="VUW49_001666"
     CDS             10823..11692
                     /locus_tag="VUW49_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7981557.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLVETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11696..12652
                     /locus_tag="VUW49_001667"
     CDS             11696..12652
                     /locus_tag="VUW49_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7981558.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12652..13416
                     /locus_tag="VUW49_001668"
     CDS             12652..13416
                     /locus_tag="VUW49_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981559.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13416..14126
                     /locus_tag="VUW49_001669"
     CDS             13416..14126
                     /locus_tag="VUW49_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981560.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14434..15090
                     /locus_tag="VUW49_001670"
     CDS             14434..15090
                     /locus_tag="VUW49_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7981561.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIISL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15198..16293
                     /gene="prfB"
                     /locus_tag="VUW49_001671"
     CDS             join(15198..15269,15271..16293)
                     /gene="prfB"
                     /locus_tag="VUW49_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7981562.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16311..17003
                     /gene="ftsE"
                     /locus_tag="VUW49_001672"
     CDS             16311..17003
                     /gene="ftsE"
                     /locus_tag="VUW49_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET7981563.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKKRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            16996..17922
                     /locus_tag="VUW49_001673"
     CDS             16996..17922
                     /locus_tag="VUW49_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981564.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
BASE COUNT         5555 a         3413 c         3949 g         5160 t
ORIGIN      
        1 tagtattatc gtagaaaatg gactcggtgc tatggataca ccggatgaga atggttatgt
       61 agcagataac tatcggatta cttacttaga ggcccacatc aaggccatgc gagatgccat
      121 ttaccaagac ggggttgact tgcttggtta tacgacttgg ggctgtatcg atccggtttc
      181 agctggcaca ggggaaatga ataagcgcta tggctttatc tatgtagatc gagataatgt
      241 aggaaacgga gctctcaaac gtagcaagaa gaagtccttc tattggtaca aggatgtcat
      301 tgatagcaat ggtgcaagca tcgggtagag cacatttgtt tactattttt ataaaatctt
      361 ctccctgctt tataagatgt aaaaaagagt tcaattcgaa ctcttttttt gctataatga
      421 ggggagaaaa atcagacagg agatcgacat gtcagaacca ttatttttac aatcagttat
      481 gcaagaaaaa atctggggtg gagccaagct acgtgatgag tttggctacg acatcccaag
      541 tgaaaaaatc ggagaatatt gggccatctc agcccatcca aatggagtct ctaaagttgc
      601 caatggtcgt tacgagggaa cagatcttgc tactttgtat gcggaacacc gtgaactatt
      661 tggcaatcgt ccagaacctg tatttccact tttgaccaag atcctcgatg ccaacgactg
      721 gctcagtgtc caagttcacc cagacgatgc ttatggactc gagcatgaag gcgaactcgg
      781 aaaaacagaa tgctggtaca ttatcgcagc ggatgaaggt tcagagatta tctacggtca
      841 caatgccaag tcaaaggaag aactccgcca gcaaatcgag gataagaact gggatgcgct
      901 gttgacaaaa gtgccagtta aagcaggaga tttcttctat gtaccaagcg gtaccatgca
      961 cgctatcggg gcgggtatct tgattcttga aacccagcag tctagcgata ccacttaccg
     1021 tgtctatgac tttgaccgta aggatgacaa gggcaattta cgtgaacttc accttgaaaa
     1081 atctatcgat gtcttgaaca ttggtgaacc cgcaaatagc cgtccagtga caatcaaagc
     1141 agatgatcta cgttccactc tccttgtatc caatgatttc ttcgcagttt acaaatggga
     1201 aattactgga aaagttaact ttgaaaagac agctgactac agcttgttga gtgtcttggc
     1261 tggtcaaggt caactaactg ttgacgggaa aaactatcca atccaaaaag ggagacactt
     1321 tatcctacca agtgatgttg aagcttggac tctggaaggg caagatttag aattaattgt
     1381 tagtcatcca taaaaaagaa agggcctgag ttcattactc aagtcctttt gattacataa
     1441 attgggcgat gaagaccacg atgatgattg gaatgacgga acgaagaaac gaagaaggaa
     1501 gagccagact tggaacagtc cttgtttcca tgctctttca tcgagatgga gttcctccat
     1561 tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa aggtagagag ctccaaatgg
     1621 catgaggaga ttggaaacca agaagtccat agcgtcaaag aaggtcttac caaaaatgtg
     1681 aacatccgcc atgacaccgt aagatagggc tgaaggaatg ccaaagacaa aggtcaaaat
     1741 tcctaaaata acactccatt tggcacgttt gctgttatcc tggttggtga tattgcccac
     1801 attgatctcc agcatcacga cagaagaagt gacagtcgca aagaggaaga ggacgtagaa
     1861 aatggttcca aaaggcatct tgtcaaagag ttgaggcaag acgataaaga gcaagctcgg
     1921 tcccccttca gactggatat tgaaggggga tcgagcttgg aaaatggcta gacctgccat
     1981 gatggatatc gagatattca tggctacgat ggagattcct gactggacta gattggtttt
     2041 cttgtccaag taagaagcat aggtcaacat gactgtaacc cctagtgaga gggcaaagaa
     2101 agattgtccc agagcataga ggagaccagc gctagtcagt tttgaaaagt ctggtttgag
     2161 gaagtaaaga accccttcca tggcatttgg caaactgaga gagcgaccga tgataacaac
     2221 aaagatgata aagagcaggg gcatcatgac tttcgaagct ctttcaatcc ctttttgaac
     2281 cccacgtgat acaatgaaga tattcaatag gataaaggcc gcttgagctc ctagggcaat
     2341 ggctggattt gaaatgattg aagtaaataa ctgagcataa tcacccgttc caccaagttg
     2401 gaacaatttc ccaaactcaa tacctagata gactagaatc catcctccga taacactgta
     2461 aaaagataag aggataaaga gggcaaaggc gccaatccaa ccgataaagt tgtacttgtt
     2521 attcttgccc agtttttcaa aggttttgat agcggaaacg ccagcactac ggccaagggc
     2581 aaactcagcc agcaggagag ggaaaccgat taaaatagtg gaaatgagaa agattagtaa
     2641 aaagcctcca ccgccattag cagcagtcat gtagggaaac ttccaaacgg aaccaagccc
     2701 gatgaccgag ccagcagatg ctagaataaa gccaagtttc gacccccatt gtgatttttc
     2761 tgacatagtt aaactctgat ctcctttttc aaaataagaa aaagctactt gagttgtcaa
     2821 atagccttct gtttgattga tagacttgac tatcctatta tgttttctaa ggtctgtcaa
     2881 gaaaatcatt ttcaagtttt cacacctttc tcaaaaaagt taaaaaattt tctcaaaaac
     2941 gcttgactct gacctaaggg gaggggttat actatcattg taaggaggaa atcatgtacc
     3001 atataaaaga agctgcgcag ctttcaggtg tctctgtcaa gaccctgcat cactatgaca
     3061 agataggact cttggtcccc ttaaagtcgg aaaacggcta tcgaacctac agtcaagagg
     3121 atttggaacg ccttcaggtc attctttact acaaatatct aggcttttct ttagagaaaa
     3181 tagcagagct gttaaaggaa gaaaggacag atttattgcc ccatttgact aggcagttgg
     3241 actatctaac tcgcgaaagg caacatctgg ataccttgat ttccaccttg caaaaaacta
     3301 ttcaagaaca aaaaggagaa agaaaaatga ccattgagga aaaattcacg ggatttagct
     3361 atcaagacaa tcaaaaatac caccaagaag cggtagagaa atatggtcaa gaagtcatgg
     3421 gacaagcgct cgaacgccaa aaaggtcacg aagacgaggc tacggccgcc tttaaccaag
     3481 tctttcaaac tttggcacaa aatcttcaag ttggtttacc tgcaacagca accgaaaacc
     3541 aagagcaagc agccaagctc ttgcaagcca ttcgcactta tggatttgac tgctctattg
     3601 aggtattcgg tcatatcggt aaaggttacg tctataaccc agagtttaag gaaaacattg
     3661 acaagtttgg ttctggaaca gcccagtaca cgtcagatgc cattgcagct tacgttcaga
     3721 caaatgcaga aaaaataggc taggaatttc ctagcctatt ttttacttca aatcataaag
     3781 ccagtcgtca ccgtttttgt agtaaaagaa ttcactgaga tcttcttcta gaaacacacg
     3841 aagcatatca gacatatcat cggttgcaag ttttagatga gaaagatttt caaagtcctc
     3901 ccaccaaact ttcccttcgt ctgaagactg gagttcacca gtaaagtgtt ctgtcttgta
     3961 aaaaaggacg acataacgat aatccttgtc gtcataccag tttttgatac cacagagttg
     4021 gggtttggaa atgatcagac cagtttcttc tttcacttca cgaatgacag catcgacaaa
     4081 ggattcgcca cgttcaacat gaccaccagg aaaagtaatg ccagaccagt cgggactaac
     4141 tcggtcttgg accaggacct tatctccgtt tttaatcata cacatgttaa caaattcgac
     4201 tgcctctctt ctgttcattc ttcacaacct ttaatcttta atcataatgc agacttcccg
     4261 ccacccagcc ggtacagagg gcagaagtga tgttaaagcc acccgtgtgg gcattgatat
     4321 ccataacttc gcctgcaaag tggaggccag gtaccagctt actttcaagg gttttaggat
     4381 tgatttcctt gagactgact ccacccttgg taacaaagga ctttgcaagg gacatttttc
     4441 cagttacagg aattttaagt tctttaatgg actggacaag ttgttctcgt tccttttcag
     4501 tcagttgttt gactttttca ggatatcctt gtacaaaaaa ttcggccaag cgttctggta
     4561 acaaggtttt taaagcgttt ttcaaggatt tttcccgatt ttcttctaga aatgtaacca
     4621 agtccttctc agaaagttga ggcaaaacat cgagtgagag aacctcccca cctttgacaa
     4681 agctagacat gcgtagggca gcaggacctg acaaaccaaa gtgggtaaag agtaaatcat
     4741 gagtgatgac atgcttacca taacttaggg tcacatcgtc cagagaaatc ccttgtaagg
     4801 ctttatgtgg aaaatctgtt aataaaggac tttcagcagc ctcaagatcg gtgatggtat
     4861 gcttaaaatg gcgagcaatc tcgtgaccaa aaccagtcga accagtcgaa ggataagact
     4921 taccacctgt tgtgacaatg agtttctcac aagtgaaggt ttgatccgct gacttaagga
     4981 caaactggtc atctactttt ttaacagaaa cgatttctat ttgagtagca acttgaccac
     5041 ctagttcggt gattttcttt tccaaagctt cgataatagt ccgagacttg tcactggctg
     5101 gaaagacgcg tccgtggtct tcgaccttaa gtttaacacc attttctgta aaaaagttga
     5161 tgatgtcatg attatcgaac tgggagaaaa cactgtaaag aaagcgtccg tttccaggaa
     5221 ttccagctag caggttgtct aagctaccat tgttggtcac attgcaacgt cccccaccag
     5281 tcccagctaa tttttttcca agtttccgat ttttttcgat gaggagggtt ttctgtccat
     5341 aaaagctact ggaaatcgta gccatcatac cagcaggtcc cccaccgatg acaatagtat
     5401 caaaatgttt catagctcta ttgtaccaca aaaaaacaag agatgatggt cacctcttgt
     5461 caagaatgca attaatcaat ttcatagccc atcagcaaac cgccctcttc tgcatagaaa
     5521 ctgcagagac cagaggttgg tagaatttta atatccgctt gtgggaaggt ttcacggatt
     5581 cgctctgaga gctgttgaca acatttttcg ttattgcgtt gggccatgac aatacggcca
     5641 ccagcatatc cagcttttac taactcatca taggcagctt gaactgattt ctttgatccc
     5701 cttgcttttt gtagcaattc gagagttcca gtttcactag cttttccgac catacgaatg
     5761 ttgagaaggc caactaccgt accgataagc ttactcaaac ggccgttctt caccaagtta
     5821 tcgactttgg ctaggacaaa gagcaactta gttttttctt gataggcggt gatagcttca
     5881 accacttctt caaaagacaa gccttggtca atcaagtcat tcaatttttc tacgagtagg
     5941 tcaacttcac caccagcaga taaactatca atcacatgaa tcttagtgtc aggatggtct
     6001 tccagataaa tattctttgc tagttgagca ctattgtgac tgccagaaag ggtacctgtg
     6061 atggttacta ggaaaatgtt tttggcacct tcaaatgctc gcaaatagtc atctgggctt
     6121 ggacaagctg attttgaagc ttctgcagtt gcatacatgg tttccatcat ttggtcaata
     6181 tcgagactgg cgtcatcaac aaagacctga tcagctactt gaatggttaa ggggacactt
     6241 acaaaggttg tgttaatagc tggtgttggc agttgacgat aatcacaacc agagtcagca
     6301 ataatcttcc aagtcataga aattctccat ctttgtcagt tatacattga caaaggttct
     6361 gtcttttttt acaattatat catgaaaact cttgaaacaa aagcatcatc cgtctgttgt
     6421 gacaagtaga aagaaaatgt tatgtctgaa cgtagaatct ctgaaaagtc tcttgaaaat
     6481 ctcagaaaat caaaccaaga atccaattta ttaaccagag aagccattga aacagccctc
     6541 ttgcaactct tggaaaaaaa ggagctgacc aagattagta tttctgaatt ggtcaaacgt
     6601 gcaggtgttt cgcgtgcggc cttttatcgc aattatgact ccaaagagga aattttagag
     6661 agcgtcttta aacgaactgt ccacaatatt atggaacagc tgcatcatta cgatttaaag
     6721 acagaccttt atttggtttg ggttcacctt ttccgggagg ccagaaagga agctagagta
     6781 attcaattgg ccttggatta ccatctggaa aaaatctttg tccaagccat gcaggaattt
     6841 ctagaaaaat accatgggaa atcaaaaggt gtcagctctt atcttcattc cttctggagc
     6901 tcagccatcg tctctgtcct tctaaaatgg atcaaggatg gcatgaaggt accagctgaa
     6961 aagattgcag atttaggttt accatttttt aaaaaataga gaaaaaggag aaaagagatg
     7021 actgaaaaaa gactggcctg ggatgagtat tttgcagccc aagccctact aattgcgaat
     7081 cgttccactt gtaaacgtgc taaagtgggc gcgattctgg taaaagataa taaggttatt
     7141 tccactggtt acaatggttc ggtgtcaggg actgagcatt gtattgatca cgaatgtttg
     7201 gtcattgaag gccactgtgt tcgcaccctt cacgctgagg tcaatgctat ccttcaaggt
     7261 gctgagcgtg gtgttcccaa aggctttaca gcctatgtaa cccattttcc ttgtctcaac
     7321 tgtacaaaac aattgcttca ggtcggctgc aagcgcgtgg tttatatcaa ccagtaccga
     7381 atggacgact acgcccaata cctttatcaa gaaaaaggga cagaattgac ccatttacca
     7441 cttgagacag tacagacagc tcttaaagag gctgatctaa tgtaaaaatt atcaaaaata
     7501 aatggcttag aaagattttt aaaccgtttt ttggtataat aagaagaata aattgaaaga
     7561 aggaattcca aaaatgggaa aaattgaagt tattaatcac ccactgattc aacacaaatt
     7621 gtcaatcttg cgtcgtacag atacttctac aaaagctttt cgtgagctag tagatgagat
     7681 tgcaatgttg atggggtatg aagtacttcg tgatcttcca ctagaagatg tggaaatcga
     7741 aacaccaatt acaaaaacag ttcaaaaaca attggcaggt aagaaattgg ccatcgtccc
     7801 aatcttgcgt gcaggtatcg ggatggttga tggtctcttg agcttggttc cagctgctaa
     7861 agttggccac atcggtatgt accgtgatga agaaacactt caaccagttg aatacttggt
     7921 gaaattgcct gaggacattg accaacgtca aatttttgta gtagacccaa tgttggcaac
     7981 aggtggctca gcaatcttgg ctgttgattc tcttaaaaaa cgtggcgcat caaatatcaa
     8041 atttgtctgc cttgtatctg ctccagaggg tgtaaaagcc cttcaagaag ctcatccaga
     8101 tgtagaaatc tttacagcag ccttggatga acgtttgaat gaacacggtt atatcgttcc
     8161 aggtcttgga gatgctggag accgcttgtt cggtacaaaa taagatcgaa aagaagaatg
     8221 acttggaaaa gtatttctag tcacgaaagg aggttgggtt tttgtttctg tctaatgaaa
     8281 gcagagcaaa aatttgacct tttttgacca agatattata atagtcttat cttcagtcat
     8341 ttgaccaaca aaattaaaac tcaaaaggag aaatgaatga ttcctgtagt tattgaacaa
     8401 acaagccgtg gagaacgttc ttacgatatt tactcacgtc ttctcaaaga ccgcatcatt
     8461 atgctgacag gtccggttga agacaatatg gctaactctg ttattgccca attgcttttc
     8521 ttggatgccc aagatagtac gaaagatatt tacctttatg tcaatacacc aggtggttct
     8581 gtttcagctg gtttggcaat cgtagatacc atgaacttta tcaaggcaga tgtccaaacc
     8641 attgttatgg gaatggctgc atctatgggg actgtcatcg catcaagtgg agcaaaaggc
     8701 aaacgtttca tgcttccaaa tgctgaatac atgattcacc aaccaatggg cggtacaggt
     8761 ggtggtaccc aacaaactga tatggctatc gctgcagaac acttgctcaa aactcgtaat
     8821 accttggaaa aaatcttggc tgaaaattca ggtcagtcaa tggaaaaagt ccatgcagat
     8881 gcagaacgtg ataactggat gagcgcccag gaaacacttg aatatggctt tattgatgaa
     8941 attatggcca acaattcatt gaactaatga tgatagaagg caaactcgac tgggtttgct
     9001 ttttttggta taatagggag agatttctta gaaagaggat ttatcatgtt tgaaaaagtc
     9061 aatcgctctg gcttgattat ctatctttac tataatcgtg atgccaaaaa actgcaggat
     9121 tatggagata ttacctatca ttccaagaaa catcgttact tacaactcta tgttccaact
     9181 caagaagtgg agcaattggt cggacgcttg agcaaggaaa aatttattaa aaaagttagg
     9241 gtttgtcata tccaagagtt ggaaacaccc tttgtgggca atctttatcg ataggaaaac
     9301 gttatcatcg aaaaagttca aggaaaacat tgacaatttt ctgacaattc ggtatattct
     9361 taacagacta tttaagaaat aaggagataa aaagatgaag aaaaaatttg ccctatcgtt
     9421 tgtggcgctt gcaagtgtag cacttcttgc agcctgtgga gaagtgaagt ctggagcagt
     9481 caacactgct ggtaactcag tagaggaaaa gacaattaaa atcgggttta actttgaaga
     9541 atcaggttct ttagctgcat acggaacagc tgaacaaaaa ggtgcccaat tggctgttga
     9601 tgaaatcaat gccgcaggtg gtatcgatgg aaaacaaatc gaagtaatcg ataaagataa
     9661 taagtctgaa acagctgagg ctgcttcagt tacaactaac cttgtaaccc aatctaaagt
     9721 atcagcagtc gtagggcctg cgacatctgg tgcgactgca gctgcggtat cgaacgctac
     9781 aaaagcaggt gttccattga tctcaccaag tgcgactcaa gatggattga ctaaaggtca
     9841 agattacctc tttattggaa ctttccaaga tagcttccaa ggaaaaatta tctcaaacta
     9901 tgtttctgaa aaattaaatg ctaagaaagt tgttctttac actgacaatg ccagtgacta
     9961 tgctaaaggg attgccaaat ctttccgcga gtcatacaag ggtgaaatcg ttgcagatga
    10021 aactttcgta gcaggtgaca cagacttcca agcagccttt acaaaaatga aagggaaaga
    10081 ctttgatgct atcgttgttc ctggttacta taatgaggct ggtaagattg taaaccaagc
    10141 gcgtggcatg ggaattgaca aaccaatcgt tggtggtgat ggattcaacg gtgaggagtt
    10201 tgtacaacaa gcaactgttg aaaaagcatc aaacatctac tttatctcag gcttctcaac
    10261 tactgtagaa gtttcagcta aagctaaagc cttccttgac gcttaccgtg ctaagtacaa
    10321 tgaagagcct tcaacatttg cagccttggc ttatgattca gttcaccttg tagcaaacgc
    10381 agcaaaaggt gctaaaaatt caggtgaaat caaggataac cttgctaaaa caaaagattt
    10441 tgaaggtgta actggtcaaa caagcttcga tgcagaccac aacacagtca aaactgctta
    10501 catgatgacc atgaacaatg gtaaagttga agcagcagaa gttgtaaaac cataatagaa
    10561 aaatgttgaa atagggaatg agcctttgac tcactccctg tttcgatatt taatactctt
    10621 cgaaaatctc ttcaaactgc gtcaacgtcg ccttggatta tatatgtgac tgacttcgtc
    10681 agtcttatct acaacctcaa agcagtgctt tgagcaacct gcggctagtt tcctagtttg
    10741 ctctttgatt ttcattgagt ataagaacct atcaaaaagt gagggaaaat cctcggaatt
    10801 ataaatagaa agagtgaatc ttatgctcca acaactcgta aatggtttga ttctaggtag
    10861 tgtttacgcg ctgttagccc taggatatac catggtttac ggaattatca agctcatcaa
    10921 cttcgcccat ggtgatattt atatgatggg agcctttatc ggttatttct tgatcaattc
    10981 tttccaaatg aatttctttg tagcgcttat tgtagctatg ctagcgacag ctattcttgg
    11041 tgtcgtgatt gagtttcttg cttaccgacc tttgcgccac tctactcgta ttgctgtttt
    11101 gattacggct attggggttt ctttcctatt ggagtatgga atggtctatc tggttggtgc
    11161 caatacccgt gccttccctc aagcgattca aacagttcga tatgatttgg gaccaattag
    11221 cttaacaaat gtgcagttaa tgattttggc catttccttg attttgatga ttttgttaca
    11281 agtcattgtc caaaagacta agatggggaa agccatgcgt gcagtatcag tagatagcga
    11341 cgcggcgcaa ttgatgggga tcaatgtaaa ccgtacgatt agctttacct tcgctttggg
    11401 ttctgctctt gcgggtgcgg ctggtgttct gattgctctt tattataact ctcttgagcc
    11461 tttgatgggg gttactccag gtcttaaatc tttcgttgcc gcagtacttg gtggtatcgg
    11521 aattattcct ggtgcggctc ttggtggctt tgtgattggt ctagtggaaa cctttgcgac
    11581 agcctttggg atgtcagatt tccgtgatgc cattgtttat ggaatcttgt tgttgatctt
    11641 gattgtccgc ccagctggta tccttggtaa gaatgtgaaa gagaaggtgt aagcgatgaa
    11701 ggaaaattta aaagttaata ttctatggtt actccttttg ttagctggct atagcttgat
    11761 tagtgtactg gtttcagtcg gagtacttaa tctattctat gtacagattt tacaacaaat
    11821 tggaattaat attattttgg ctgttggtct caacttaatc gttggttttt caggacaatt
    11881 ttcacttggt catgctggtt tcatggcgat tggtgcctat gcagcagcta ttattggttc
    11941 taaatcacca acctacggtg ccttctttgg agctatgctt gtaggggctt tactttcagg
    12001 agcagttgcc ttacttgtcg gcattccaac cttgcgcttg aagggggact atcttgcggt
    12061 agcaactcta ggtgtttctg aaattatccg tatctttatc atcaatggtg gaagccttac
    12121 aaatggtgcg gcaggtatct tagggattcc taactttaca acttggcaaa tggtttactt
    12181 ctttgtcgtg attacaacca ttgcaacctt gaacttcttg cgtagcccaa ttggtcgttc
    12241 aaccctctct gttcgtgaag atgaaatcgc tgctgagtca gttggggtta atacgactaa
    12301 aattaaaatc atcgcttttg tctttggtgc cattactgca agtattgctg ggtcacttca
    12361 ggcaggattt atcgggtctg ttgtaccgaa agattacacc ttcatcaact caatcaacgt
    12421 tttgattatt gttgtatttg gtggactcgg ttccattaca ggtgcgattg tttcggctat
    12481 tgttctggga attttgaata tgcttctcca agatgttgct agtgtgcgta tgattattta
    12541 cgctttggcc ttggtattgg taatgatttt cagaccaggt ggactccttg gaacgtggga
    12601 actgagccta tcacgtttct ttaaaaaatc taagaaggag gaacaaaact aatggcatta
    12661 cttgaagtaa aacagttaac caaacatttt ggtggtctaa cagctgttgg agatgtgact
    12721 cttgaattga acgaagggga actggttgga ttaatcggtc caaacggagc tgggaaaacc
    12781 acccttttca accttttgac cggtgtttat gaaccaagcg agggaacagt aaccctagat
    12841 ggtcaccttt tgaatgggaa atcaccttat aagattgcct ctttgggact tggacgtact
    12901 ttccaaaata tccgtctttt taaagattta acagttttag acaatgtttt gattgctttt
    12961 ggaaaccatc acaaacagca tgtttttact agtttcttac gcttaccagc tttttacaag
    13021 agtgaaaaag aattaaaggc taaagctttg gaattgttga aaatctttga tttagatggt
    13081 gatgcagaga ctcttgctaa aaatctttcc tacggacaac aacgtcgttt ggaaattgtt
    13141 cgtgcccttg ctacggaacc taaaattctc ttcttagatg aaccagcagc aggtatgaac
    13201 ccacaggaaa cagccgaatt gactgagtta attcgtcgta tcaaagatga atttaagatt
    13261 acaatcatgt tgattgaaca cgatatgaat ctggtcatgg aagtaacaga acgtatctac
    13321 gtacttgaat atggccgttt aattgctcaa ggaactccag acgaaattaa gaccaataaa
    13381 cgcgttatcg aagcttatct aggaggtgaa gcctaatgtc tatgttaaaa gttgaaaatc
    13441 tttctgtgca ttacggtatg atccaagcag tccgtgatgt aagctttgaa gttaatgaag
    13501 gagaagttgt ttcccttatc ggtgccaacg gtgcaggtaa gacaactatt cttcgcacct
    13561 tgtcaggttt ggttcgacca agttcaggaa agattgaatt tttaggtcaa gaaatccaaa
    13621 aaatgccagc tcagaaaatc gtggcaggtg gtctttcaca agttccagaa ggacgccacg
    13681 tctttcctgg cttgactgtt atggaaaatc ttgaaatggg agctttctta aagaaaaatc
    13741 gtgaagaaaa tcaagctaac ttgaagaagg ttttctcacg ctttcctcgt cttgaagaac
    13801 ggaagaacca agatgcagct actctttcag gaggggaaca acaaatgctt gccatgggac
    13861 gcgctcttat gtcaacacca aaacttcttc ttttagatga accatcaatg ggacttgccc
    13921 caatcttcat ccaagagatt tttgatatca ttcaagatat tcagaagcaa ggaacaaccg
    13981 tcctcttgat tgaacaaaat gccaataaag cactcgcaat ctctgaccga ggatatgtat
    14041 tggaaacagg aaaaatcgtc ctatcaggga caggaaaaga actcgcttca tcagaagaag
    14101 tcagaaaagc atatctaggt ggctaaaaca atccagtgga ttgttttagt cggcagatga
    14161 agattacgaa gtaatcatca atatagtccg ggggaccttt ttagccggta gattgagatt
    14221 gcaaacaaat ctgcatctac attgaaagct taatttctaa taattgaaaa aatcgaatga
    14281 aacgtatctt accttcattc agggagctcg attttagagc tctttttgct agcttattca
    14341 tacttttttg aatttcgaaa aagaaatgta agcgtttgat agatttacaa aaagattgta
    14401 taatggggat aagaatagaa aaggagaagt ctcatggcag ttaaagattt tatgacccgc
    14461 aaggtagttt atattagtcc agatacaaca gtatctcatg cagcagattt gatgagagag
    14521 caaggattgc accgcttgcc tgttatcgaa aatgatcaat tggttggttt ggtgactgag
    14581 ggaaccattg cacaagcaag tccatctaaa gcaacaagtc tttctatcta tgagatgaat
    14641 tatcttctga ataagacaaa agtaaaagat gtcatgattc gcgatgttgt cactgtctca
    14701 ggctatgcta gtctagaaga tgcaacttat ctgatgttga aaaataagat tggtattctc
    14761 cctgtcgtag ataaccatca agtatacgga gttattactg accgtgacgt tttccaagcc
    14821 tttcttgaaa ttgcaggtta tggcgaagaa gggattcgtg tacgctttgt tacagaagat
    14881 gaagttggtg ttcttggaaa aattatttct ttgattgtag aagaaaattt gaatatatcc
    14941 catacagtca atattccgcg taaggatggt aaggtcatta tcgaagtgca aatcgatgga
    15001 tcaattgatt taccagcctt gaaagaaaaa tttgaagcaa atggtattca agtggaagaa
    15061 atcgctcgca cttcagcaaa agtcttgtaa gaagggaagc ccaaaggctt cttttttcat
    15121 gaaaagggga ttagagcaaa agatggaaag aaatgataaa atatgctata atgaaataat
    15181 gtaaaaaagg agtatttatg gacatttcag taattcgtca gaaaattgac gcaaatcgtg
    15241 aaaaattagc ttctttcagg gggtctcttt gacctcgaag ggctagagga agagattgcc
    15301 atcttggaaa acaagatgac agaacctgat ttttggaacg ataatattgc ggcccaaaaa
    15361 acgtcgcaag aattgaatga attaaaaaac acttacaata ccttccataa gatggaagag
    15421 ttgcaggatg aagtcgaaat tttattggat tttttggctg aagacgagtc agtgcatgat
    15481 gaactggtag cgcagttagc cgaacttgat aagataatga ccagctacga gatgactcta
    15541 ctcttgtcag aaccttatga ccacaacaat gccatcttgg aaatccatcc aggttctggt
    15601 ggtactgagg cgcaggactg gggtgatatg ttgcttcgta tgtatactcg ttatggtaat
    15661 gctaaaggct ttaaagtgga agtgttggat taccaagcag gtgatgaggc tggtattaag
    15721 tcggtaactt tatcatttga agggcctaat gcctatggtc tcctcaagtc agaaatgggt
    15781 gttcaccgct tagtgcgaat ctcaccattt gactctgcca aacgtcgcca tacctctttc
    15841 acatctgtag aagtgatgcc agaattggat gatactattg aagtggaaat ccgtgaagat
    15901 gatatcaaga tggatacctt ccgttcaggt ggtgccggtg gacaaaacgt caataaggtt
    15961 tcaacaggtg tacgtttaac ccacattcca actggaattg ttgtccaatc aacagtggat
    16021 cgtacccagt atggaaatag agatcgtgcc atgaagatgt tgcaggctaa gctctatcaa
    16081 atggagcaag agaagaaggc tgcggaggta gattctctca aaggtgagaa aaaggagatt
    16141 acttggggaa gtcaaatccg ttcttatgtc ttcacgcctt atactatggt aaaagatcac
    16201 cgaactagct ttgaggttgc tcaggtagat aaggttatgg atggggacct agatggtttt
    16261 atcgatgctt atctcaagtg gcgaattagc taagatagaa aggaactcac atgtcaatta
    16321 ttgaaatgag agatgtcgtt aaaaaatacg acaacggaac gactgctcta cgcggtgttt
    16381 cggtcagcgt tcaaccgggg gaatttgctt acatcgtagg accttcagga gcagggaagt
    16441 caacttttat tcgttctctg tatcgtgaag taaaaatcga taaaggaagc ctatcagttg
    16501 ctggttttaa tctggttaag atcaaaaaga aagatgtccc gcttctacgt cgtagtgttg
    16561 gggttgtctt tcaggattat aaattgttac caaagaaaac tgtctatgaa aatattgctt
    16621 acgctatgga agtaatcggg gaaaatcgcc gtaatatcaa aaaacgagtg atggaagttt
    16681 tggacttggt tggattgaag cataaggttc gttctttccc aaatgaactc tcaggtgggg
    16741 agcaacagcg gattgcgatt gcgcgtgcaa ttgtaaataa tcccaaagta ttgatagctg
    16801 atgagccaac aggaaatctg gatccggata attcatggga aattatgaat ctcttggaac
    16861 ggattaacct acaaggaaca actattttga tggcgactca taatagccag attgtaaata
    16921 ccttgcgcca ccgtgtcatt gccattgaaa atggccgtgt cgttcgtgac gaatcaaaag
    16981 gagagtatgg atacgatgat tagtagattt tttcgccatt tatttgaagc cttaaaaagt
    17041 ttgaaacgaa atggttggat gacagtagct gctgtcagtt cagtcatgat tactttgacc
    17101 ttggtggcaa tatttgcatc tgttattttc aatacagcga aactagctac agatattgaa
    17161 aataatgtcc gtgtagtagt ttatatccga aaggatgtgg aagataatag tcagacaatt
    17221 gaaaaagaag gtcaaactgt tacaaataat gactaccaca aggtatatga ttctttgaag
    17281 aacatgtcta cggttaaaag tgttaccttt tcaagtaaag aagaacaata tgaaaaatta
    17341 accgagataa tgggagataa ctggaaaatc tttgaaggag atgccaatcc tctctatgat
    17401 gcctatattg tagaggcaaa cactccaaat gatgtaaaaa ctatagccga agatgctaaa
    17461 aaaattgaag gtgtctctga ggttcaagat ggcggtgcca atacagaaag actcttcaag
    17521 ttagcttcat ttatccgtgt ttggggacta gggattgctg ctttgttaat ttttatcgca
    17581 gttttcttga tttcaaatac cattcgtatt accattattt cccgcagtcg cgaaattcaa
    17641 atcatgcgct tggtcggagc taaaaacagt tatatccgtg gaccgttctt gttagaagga
    17701 gcctttatcg gtttattggg agctatcgca ccatctgttt tggtctttat tgtttatcaa
    17761 attgtttacc aatctgtcaa caaatcgttg gtagggcaaa atctatccat gattagtcca
    17821 gatttattta gtccgttgat gattgcccta ctatttgtga ttggggtttt cattggttca
    17881 ttgggatcag gaatatccat gcgccgattc ttgaaggttt aggtaaaata gctgctttta
    17941 tgaggagatt gtaaaatctc cttttttgct acaagagttt ttgaaaagag atgcgtagaa
    18001 gaaaagagct tccaaagaag tcccccagag aagacttctt gatggctaat ttgaacttga
    18061 aatttggcgc actagaa
//
LOCUS       DAVDWM010000043        17899 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVDWM010000043 DAVDWM010000000
VERSION     DAVDWM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.043"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(98..1033)
                     /gene="birA"
                     /locus_tag="VUW49_001674"
     CDS             complement(98..1033)
                     /gene="birA"
                     /locus_tag="VUW49_001674"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HET7981565.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAGSLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1030..1890)
                     /locus_tag="VUW49_001675"
     CDS             complement(1030..1890)
                     /locus_tag="VUW49_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7981566.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTVA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1998..4160
                     /locus_tag="VUW49_001676"
     CDS             1998..4160
                     /locus_tag="VUW49_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET7981567.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKNVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            4195..5454
                     /locus_tag="VUW49_001677"
     CDS             4195..5454
                     /locus_tag="VUW49_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7981568.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQGLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5468..6334
                     /locus_tag="VUW49_001678"
     CDS             5468..6334
                     /locus_tag="VUW49_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7981569.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKAGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANANAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6348..7181
                     /locus_tag="VUW49_001679"
     CDS             6348..7181
                     /locus_tag="VUW49_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7981570.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7182..7262
                     /locus_tag="VUW49_001680"
                     /pseudo
     CDS             7182..7262
                     /locus_tag="VUW49_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7539..8981
                     /gene="gtfA"
                     /locus_tag="VUW49_001681"
     CDS             7539..8981
                     /gene="gtfA"
                     /locus_tag="VUW49_001681"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET7981571.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINCHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            9119..10312
                     /locus_tag="VUW49_001682"
     CDS             9119..10312
                     /locus_tag="VUW49_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7981572.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10998..12977
                     /locus_tag="VUW49_001683"
     CDS             10998..12977
                     /locus_tag="VUW49_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7981573.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13044..14540
                     /locus_tag="VUW49_001684"
     CDS             13044..14540
                     /locus_tag="VUW49_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981574.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14540..15466
                     /locus_tag="VUW49_001685"
     CDS             14540..15466
                     /locus_tag="VUW49_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981575.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15475..16542
                     /locus_tag="VUW49_001686"
     CDS             15475..16542
                     /locus_tag="VUW49_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981576.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16553..17479
                     /locus_tag="VUW49_001687"
     CDS             16553..17479
                     /locus_tag="VUW49_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981577.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(17555..>17899)
                     /locus_tag="VUW49_001688"
     CDS             complement(17555..>17899)
                     /locus_tag="VUW49_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981578.1"
                     /translation="YSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQT
                     VFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNI
                     KKERTKFVLSRA"
BASE COUNT         5262 a         3182 c         3769 g         5686 t
ORIGIN      
        1 ttatttttag agccaataaa acttcagatc cgtaaaactt aattgaatta ctaatctgaa
       61 gttattttta tatcgtttat attataattg tgttatttta cttccaacta ttgagagaaa
      121 tttcgccact atttagccag atttcttttc cgttatcaca ttgaactaaa agttttccat
      181 tttctgagat gtctttagca agtcccttgt agtctttttg ctctagtgtg aaagtgactt
      241 cttttcctag aatgaatgac tgttttttgt ataggtataa tagctcttct gctggtgttt
      301 cgaagaaagc acgccagatt tctatgatca attcattcct tgttatagga gctgtagctt
      361 taaataagct gccagctttt tcttttaatt cctgagggaa gtctttaata gtgaaattga
      421 tacctactcc aataatgata tctgtgacta agccagtttc tacagaggtc attgcttcag
      481 taaggattcc tccaatttta tgattgttta gatagatatc attgacccat tttatgtcga
      541 catctattaa agttaggttc ttaatggctt tgtagacagc tccagctaca agtagtgtgt
      601 aggatggtaa tttgtcatag gggagatttg gtttaagatg gagtgtcata taaataccac
      661 cttgtggtga gtagaaggaa cgttgaaaac ggcctcggcc tgctgtttga taggaagcta
      721 gatagagggt atttgcttca tggcctaaat caattgcttc ttttgcatct agttgtgttg
      781 attttgtttc gggtttaaag ctgactttaa ttggaagatt ttcttctaga atctctggaa
      841 gaataaggtc accattcatc agtttatatc ctctattttt gatactatca atttcaatgc
      901 cttcttgttc tagtcgcttg atggctttcc aaattgctgt tcggcttagg gatagttttt
      961 ctgcgatttt ttctccgctg atatagtcgg tttctttaga taggatttgg tagacagctt
     1021 ggtaggattt cataatgttg cctttcttac tagttggtat tgagagtatt cttttcttgt
     1081 atgacttgga gtctgattaa agtattgttt ataagctttc gaaaaatgga gtggatctga
     1141 aaaacctacc gagtatgcaa ttaccttgat ggactcttgg gtattttcga gaagttgtct
     1201 agctcggtgc attcgaacgt agagtaggta ttctttgggt gataaggtat taaattcttt
     1261 gaatacgctt gataagtagc ttctgtgaac ggatagttct tttgctaaat cttgaattgt
     1321 aagtgattga ggatagtggc tatcaattaa tcgtttgcat tcaagataga gttggtgggt
     1381 tgatgaaata ttcttttttt tctgattggg agcaacagtt cccagatgaa acatcagttc
     1441 atgaagttgt cccatgatat ggagttgagc taattcactt gattttgtaa tctgagcgaa
     1501 gcggacaatg tctgagatga gttttgcagt agtctgggta tgacaagttt cagattggat
     1561 gagataggat tgatcagaaa tttgagaaag agcaaaataa tcaggggatt tccctccagt
     1621 gattcctaac cagtagtagg cccaaggttc tttactatct gcttgataaa aggttagttc
     1681 ctctggtttt aatagaaaga aatctccttc ttttaaatca acaattttac ccttgtaatg
     1741 aaattttcct tgtcctttag taatgtaatg taggacgtat gtatcacgaa tggctggacc
     1801 aaaagagtaa ttaggtgtgc attcctcata tccataaaag cttagggcaa ggtcaattgt
     1861 tccagtctgg tattctgaaa aaactagcat gtgctacctc ctacctaaca ttttaccata
     1921 ttcttgagac atttttctat tttggaaagc gatttcaggt gataaaatat aatcaataaa
     1981 gtgatgaggt gaagtaaatg ggagttagga tagagaataa tctattttat gttgagagta
     2041 aaaatctaag tttgattatt gaaaatcgaa atggctactt acttttgaaa catttaggaa
     2101 agactattaa gaactataaa ggttccaata gtgtttatga acgagaccat gccttttcag
     2161 gaaatccaac ggctactaat cgaaccttta gtttagatac tcagcgacag atttttggac
     2221 aacatggctt aggagatttt aggaaaccaa ctatacaggt tcagcatagt gtaactgaag
     2281 taacagactt tcgatttgta gaagcaaaga ttttaaaagg tcagaatggt ccacagggct
     2341 taccttctcc acatagcatg gacgatacag agactcttgt cttaatgtta gaagattcta
     2401 aggctcaact tagtctgact ttgtattata ctacttttaa taatgatgcg actattgcta
     2461 gctacagtaa attagataat aatagtaatc aggaagttgt catccataaa gatttttctt
     2521 ttatggctga ttttccagct gcagattacg aaatagtaac tctgcagggt gcttatgctc
     2581 gtgaaaagac tgttagacgt caacaggtag aacaaggaat cttttcgatt agctcaaacc
     2641 gaggtgcttc tggtcatgct caaacaccag ctcttctact atgcgaacaa ggagtcacag
     2701 aggatgctgg gaatgtgttt gctattcaac taatgtatag tggcaacttt gaagcttttg
     2761 ttcaaaaaaa tcaattgaat gaagttcggg tggctattgg cattaatcca gaaaactttt
     2821 cttggaagtt agctcctgag gaatactttg aaacaccggt agctttagtg acccattcag
     2881 atcagggatt aactggtatt agtcatgaaa gtcagaattt tgtactgaag cacattatgc
     2941 taagtgaatt ttctaaaaaa gaacgtccaa ttctaatcaa taactgggaa gctacttact
     3001 ttgactttca gagagaaaaa ctgttagagt tagcagatga agctaagaaa gttggcattg
     3061 aactttttgt attagatgat ggttggtttg gcaatcgttt tgatgataat cgtgctttag
     3121 gtgattgggt tgttaatgag gaaaaactgg gtggaagtct agaaagtctg atttcagcta
     3181 tccatgaaag aggtttgcag tttggacttt ggttagaacc cgaaatgatt tctgtagata
     3241 gtgatttgta tcgtcgacat cctgactggg ctattcaggt tcctgactat gagcatactt
     3301 attctcggaa tcaattagta cttaatcttg ccaatcctca ggtagtagaa tacttgaaaa
     3361 atgtcttaga tcaactccta tcttatcatg atattgatta cattaaatgg gatatgaacc
     3421 gcaatatcac taagctaggg aatggattaa cttatctaga gacacagatg caatctcatc
     3481 agtacatgct ggggctttac gaactcgttt cttatctgac agagaagcac agccatattc
     3541 tctttgagtc ctgctctggt ggtggtggac gaaatgatct tggtatgatg cgctatttcc
     3601 cacaagtctg ggctagtgat aatactgatg ccattgcacg tttaccaatt caatacggtt
     3661 catcctatct ctatccaacc atttctatgg gggctcatgt gtcagcagta ccgaatcatc
     3721 agatgggacg aatgacacca ttagaaacac gtggccttgt agcaatgatg ggaaatttgg
     3781 gctatgaact tgatttgaca aatttatcag atgaagagaa agctacgatt gctaatcagg
     3841 tgaacttgta taaagaatta cgaccagtag ttcagttagg acagcagtat agactaatta
     3901 atcctgatac tgtatccaat gaagcagctg tacaatttaa ttacggaaat caaacgattg
     3961 taacctacgt tcgcgtttta tctgttgtag agaccatgga aacaacttta aaattaaaag
     4021 atttggatga agagggacta tataaattac aggaaaatgg cgaagtttac tcaggtgcag
     4081 aactaatgta tgcgggctta actgttatct tatcccaagg agattttttg agtagacagt
     4141 atatttttag aagactatga taagggtgta taaatttata aaggatgctt tctaatggaa
     4201 tggtataaaa aaatcggact tcttgcaact acaggtttag ctttggttgg gctcggcgct
     4261 tgctccaact atggtaaatc tgcggatggc acagtgacca tcgagtattt caaccagaaa
     4321 aaagaaatga ccaaaacctt ggaagaaatc actcgtgatt ttgagaagga aaaccctaag
     4381 atcaaggtca aagtcgtcaa tgtaccaaat gctggtgaag tattgaagac acgcgttctc
     4441 gcaggagatg tgcctgatgt ggtcaatatt tacccacagt ccatcgaact gcaagaatgg
     4501 gcaaaagcag gtgtctttga agatttgagc aacaaagact acctgaaacg cgtgaaaaat
     4561 ggctacgctg aaaaatatgc tgtaaacgaa aaagtttaca acgttccttt tacagctaat
     4621 gcttatggaa tttactacaa caaagataaa ttcgaagaac tgggcttgaa ggttcctgaa
     4681 acctgggatg aatttgaaca gttagtcaaa gatatcgttg ctaaaggaca aacaccattt
     4741 ggaattgcag gtgcagatgc ttggacactc aatggttaca atcaattagc ctttgcgaca
     4801 gcaacaggtg gaggaaaaga agcaaatcaa taccttcgtt attctcaacc aaatgccatt
     4861 aaattgtcgg atccgattat gaaagatgat atcaaggtca tggacatcct tcgcatcaat
     4921 ggctctaagc aaaagaactg ggaaggtgct ggctataccg atgttatcgg agccttcgca
     4981 cgtggggatg tcctcatgac accaaatggg tcttgggcga tcacagcgat taatgaacaa
     5041 aaaccgaact ttaagattgg gaccttcatg attccaggaa aagaaaaagg acaaggctta
     5101 accgttggtg cgggagactt ggcatggtct atctcagcca ccaccaaaca tccaaaagaa
     5161 gccaatgcct ttgtggaata tatgacccgt ccagaagtca tgcaaaaata ctacgatgtg
     5221 gacggatctc caacagcgat cgaaggggtc aaacaagcag gagaagattc accgcttgct
     5281 ggtatgaccg aatatgcctt tacggatcgt cacttggtct ggttgcaaca atactggacc
     5341 agtgaagcag acttccatac cttgaccatg aactatgtct tgaccggtga taaacaaggc
     5401 atggtcaatg atttgaatgc cttctttaac ccgatgaaag cggatgtgga ttaggagtag
     5461 agagactatg aaaaaagtat tacaaaaata ttgggcatgg gcttttgtgg tcatccccct
     5521 cttgttacaa gcaattttct tctatgtgcc gatgtttcaa ggagcctttt acagttttac
     5581 caactggaca ggattgactt ataactacaa atttgttggc ttaaacaact ttaagctcct
     5641 cttcatggat ccaaaattca tgaatgcgat tggctttacc gcaatcatta cgattgccat
     5701 ggtggttggt gagattgcac tcgggatctt cattgcgcgt gtcttgaatt ctaaaatcaa
     5761 aggccaaacc ttcttccgtg cttggttctt cttcccagct gttttatctg gtttgacagt
     5821 ggctttgatc ttcaagcaag tcttcaacta cggtcttcca gcgattggga atgcccttca
     5881 tattgaattt ctccaaacca gtcttttagg gactaagtgg ggagcaatct ttgcggctgt
     5941 ctttgtcctt ctttggcaag gggtggctat gcccatcatc atcttcctag ctggtttgca
     6001 atctattcca actgagatta cagaggcagc aaggattgat ggtgcgacta gcaagcaagt
     6061 tttctggaac attgaattgc cttacttgct accaagtgtc tctatagtct ttatcctagc
     6121 cctaaaagct gggctgactg cctttgacca agtctttgcc atgaccggtg gtggtccaaa
     6181 caatgccaca acctcacttg ggctcttggt ttataactat gcctttaaaa acaaccaatt
     6241 cggttatgcc aatgccaatg ccgtaatctt gttcttctta attgtagtga tttcgatcat
     6301 ccaattgaga gtatctaaga aatttgaaat ttaagaggag aagcatgatg aaacaagatg
     6361 aaagaaaagc cctgattggc aaatacattc tattgattct aggatcggtt ctgattttag
     6421 tgccgctcct tgctaccctc tttagttcct tcaaacccac taaggatatt gtagataatt
     6481 tctttggctt tccaaccaac ttcacatggg acaactttag ccgtctctta gctgatggga
     6541 ttggaggcta ttattggaac tctgtcgtca tcactgtctt gtctttactt gcagtaatga
     6601 tctttatccc tatggcagcc tactccatcg ctcgcaatat gagtaaaaga aaagccttta
     6661 ccatcatgta taccctctta atcctcggaa tcttcgtacc tttccaagtc atcatgattc
     6721 cgattacggt tatgatgagt aaactcggtt tggctaatac ctttggtttg atcttgctct
     6781 acttgaccta tgcgattcca cagaccctct ttctctatgt tggctatatc aaaatctcga
     6841 ttccagaaag tctggatgaa gcagcagaga tcgatggggc taatcaattt acaacctatt
     6901 tccgcatcat cttcccaatg atgaaaccga tgcatgcgac aaccatgatc atcaatgccc
     6961 tttggttctg gaatgacttc atgttgccac tccttgtctt gaaccgggat tccaaaatgt
     7021 ggactctgcc tttgttccaa tacaactacg caggccaata tttcaacgac tacggaccaa
     7081 gctttgcctc ttacgtggtc ggcattatca gtatcaccat tgtctatctc ttcttccaac
     7141 gccatatcat ttcaggaatg agcaacgggg cagtgaagta atactcaatg aaaatcaaag
     7201 agcaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt gtggatgaaa
     7261 ctgacgaagt cagtaaccat acctacggta aggtgaagct gacgtggttt gaagagattt
     7321 tcgaatagta taagaagagc tcatggaatc tatagtcttt gacacagagg aagagttcag
     7381 cttgttgata attctatgta gaaaacaaga aagtagtact ttgtttatca cagtctatat
     7441 tagtacgagt ttgatgataa atagagattt aaaatgaatt ttatttatga agccagatca
     7501 tatgagactt ggcttcaatt agaaaaagga gagtaatgat gccaattcaa aataaaacca
     7561 tgttgattac ctattctgat agccttggaa ataatcttaa agacttatat gataatttgg
     7621 aagagcattt tggagatgct attggaggag ttcacctttt accatttttc ccatcaacag
     7681 gtgatcgtgg atttgcgcca gttgactacg acgaagtgga ctcagctttt ggtgattggg
     7741 aggatgttaa gcgtttaggt gagagatatt atcttatgtt tgattttatg attaatcata
     7801 tttctcgtca atccaagtat tataaggact atcaagaaaa acatgaagcc agtgaattta
     7861 aagatctctt tttaaactgg gataagtttt ggccagaaaa ccgtccgaca cagtctgatg
     7921 tagatttaat ttacaagcgt aaggatcgtg caccaaagca agagattgtg tttgaagatg
     7981 gttcagtgga acatttgtgg aatacttttg gtgaggagca gattgatctt gatgtgacca
     8041 aagaagtaac tatggaattt atccgtaaga ccattcagca cttggcaagt aatgggtgtg
     8101 atttgattcg tctagatgcc tttgcttatg cagtgaagaa attggatact aatgatttct
     8161 ttgtggaacc agatatttgg gatttattgg acaaagttcg agatatcgct gctgagtatg
     8221 ggacagagct tttacctgag attcatgaac actattcgat tcagtttaaa atagcagacc
     8281 atgattacta tgtttatgat tttgctcttc caatggtgac actttatact ctttacagtt
     8341 ccagaacaga gcgtttggct aagtggttaa agatgagccc gatgaagcaa tttacgacgc
     8401 tagataccca tgatgggatt ggagtagtag atgtcaagga tatcctgacc gatgaggaga
     8461 ttgactatgc ttcaaatgaa ctctataagg ttggagccaa tgtcaaacgt aagtactcta
     8521 gtgccgagta taacaactta gatatctacc aaatcaattc aacctactat tcagcgcttg
     8581 gagatgatga tgtcaagtat tttctcgctc gtctaattca agcttttgcc ccaggtattc
     8641 ctcaggttta ctatgtgggt ctattagcag gcaagaatga cttgaaatta ttagaagaaa
     8701 ctaaagaagg tcgaaatatt aattgtcatt actatagcaa cgaggaaata gcaaaagaag
     8761 tgcaacgacc tgttgtgaag gcccttctca atctattttc tttccgtaac cgttcagaag
     8821 cctttgatct agaagggact actgagatag agacaccaac agctcacagc attgtaatca
     8881 aacgtcaaaa taaagataag tccgtaacag cagtagtaga aattgatttg caaaatcaga
     8941 cttatcgggt aattgagaat ggagttgaag tatcattttg aagccttgat atggtggata
     9001 aaatagggtt tttattttgg aaaacgtttc ctttgttttc aaattgctaa aaaagtggta
     9061 caatatagga tagcttacta ttatctgaat cagctgattt ggagagaaag gattcatttt
     9121 gaaatcaata ggctttattg aaaagctgaa ggggttgtct agtaaagagc tgattttatt
     9181 gggaattatc ctaagtatct ttttaccctt ttatcttttt gtagttgtac tctgtttata
     9241 tattatcagt ttgattttta caggagacat gaaaagtatt cttcagaaaa tgggggagca
     9301 tccgatgctg cttctttttc ttggctatag tactgttata tccattcttg cacaaaattg
     9361 gatgggtctt gtggcttcag taggaatgtt tctatttact attttctttt tgcactatca
     9421 gtcgatttta tcccataaat tctttcgatt gattttgcag ttcgtcttgt ttggtagtgt
     9481 cttgtcagct gcttttgcca gtttagaaca tttccaaatt gtgaagaaat ttaactatgc
     9541 ttttctttca cccaatatgc aggtgtggca tcagaaccgg gcagaagtga ccttctttaa
     9601 tcctaattat tatggaatta tttgttgttt ctgtattatg attgctttct atctgtttac
     9661 aacgaccaag ttgaattggt tgaaagtatt ctgtgtgatt gcaggctttg ttaatctctt
     9721 tggtttgaac tttactcaaa atcgaactgc ctttcctgct attatcgctg gagcaattat
     9781 ctatctcttt acgacgatta aaaactggaa ggccttttgg cttagtattg gggtcttcgc
     9841 gattggtttg agtttcctct tttctagtga tttgggagtt cgaatgggta ctttagactc
     9901 ttctatggaa gaacgcattt ctatctggga tgctgggatg gccttgttta agcaaaatcc
     9961 tttttggggt gaagggccat tgacctatat gcactcttat cctcggatac atgctcctta
    10021 tcatgaacat gcccacagtc tttatattga tacgattctg agttacggaa ttgtgggtac
    10081 cattttatta gttttgtctt ctgttgctcc tgttcgcttg atgatggata tgagtcagga
    10141 gtcggggaaa cgtccgatta tcggccttta tctatctttc cttacagtgg ttgctgtgca
    10201 cggaattttt gacttggctc tcttctggat tcagtcaggc tttattttct tgctagttat
    10261 gtgcagtatt ccattggagc atcgaatgtt ggtatcggac atgacggatt aagttttata
    10321 agtttgaaat cttctacctt gggtaggaga tttttttata cgatgggcat gtcgtgcatc
    10381 tgaggtgtaa gtcctctgta gggatccact accggtgaat acaatagcgc tccccaagcc
    10441 tgactatcgt gaggtagcgg attaaaatgg tctggggata gatcgtttta agtctgacgc
    10501 aggaaataag aattgtcaga gaaagcaata gtgaaatcgt aggctatgaa tagaaacgtg
    10561 atagcgagga gcatatagtg gataaggtag cgagaaattt taaagtataa aagatagtct
    10621 taatctatat atgtaaatca caaaagtaat tgattcggaa taaggttggt gtgaaaagac
    10681 atagtaaact gaatcttgaa tactagaatg atacttctac aatattgtta taatttaatt
    10741 tgaattccaa aatcacattg ttccgtccgt atttcatttt actgtatgtc ttcctaagct
    10801 ccaaaatttc tgtgtcaaat ttcctatttt cactgtagtc tttgacactt cgaacgacaa
    10861 tttgaatcat atattcaaaa aaaaatgaaa aatttgtgcc aacaagccta aatattctga
    10921 aaatttgttt gatttttaat ggataatgtg atataatggt tgtcaatcta taattcacat
    10981 aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat
    11041 tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc atctgattca tcagccccta
    11101 aagcttatgg ctatgtttat acagcagacc cagaaacctt ggactacctg atttcaagta
    11161 aaaatagtac aacagtagtg acttcaaatg ggattgatgg tttattcact aacgataatt
    11221 acggtaatct tgctcctgca gttgcagagg attgggaagt ctctaaggat ggtttgacct
    11281 acacttataa gattcgtaaa ggggttaaat ggtttacctc tgatggagaa gaatatgcag
    11341 aggtgacggc taaagatttc gtgaacggtt taaaacacgc agcagataaa aaatcagaag
    11401 ctatgtattt agctgaaaat tcggttaaag gcttggcaga ttatctatca ggaacttcaa
    11461 cagatttttc aacagttggt gtcaaggcgg ttgatgatta tacgttacaa tacactttga
    11521 accagcctga accgttctgg aactctaagt tgacctattc tattttctgg cctctgaatg
    11581 aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc aacagatccg acatccttgc
    11641 tttataatgg tccattcttg ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa
    11701 aaaatgagca atattgggat aaagaaaatg tccacctaga tactatcaat ctagcttact
    11761 atgatggatc agatcaggag tcgctagagc gtaacttcac tagtggagct tatagttatg
    11821 cccgtcttta ccctaccagc tccaactatt ctaaggttgc agaagaatac aaggacaata
    11881 tctattacac acaatcaggc tctgggattg ctggtctggg tgtgaatatt gatcgccaaa
    11941 gttacaacta tacttctaaa actacagatt cagagaaagt agctactaag aaggcattgc
    12001 ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga tcgctcagct tactcagctc
    12061 aaatcaatgg taaagatgga gcagctttag cagttcgtaa tttatttgta aaaccagact
    12121 ttgtttcagc tggtgagaag acctttggtg atttagtcgc tgctcaactt cctgcttatg
    12181 gtgatgagtg gaaaggtgtg aatttagctg atgggcagga tggtttattc aatgctgaca
    12241 aggccaaggc agagtttgcg aaagctaaga aagctttaga agcagacggc gttcagtttc
    12301 ctattcatct ggacgttcca gtagaccaag catcaaaaaa ctacatatct cgtattcagt
    12361 cctttaaaca atctgtagaa acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac
    12421 aaatgacaag tgatgaattc cttaatatta cttactatgc tgccaatgct tcatctgagg
    12481 attgggatgt atcgggagga gtttcatggg ggccagacta tcaagaccca tctacttacc
    12541 tggatatttt aaaaacaact agcagtgaaa ctacaaaaac atatttagga tttgataatc
    12601 caaatagccc ttcagtagtt caagttggtt tgaaagaata cgataaatta gttgatgaag
    12661 ctgccaaaga gacaagcgac ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat
    12721 ggttgacaga tagttcactc tttattcctg ctatggcttc ttctggtgca gcaccagtgc
    12781 tttcacgaat tgttccattt actggagctt ctgcgcaaac aggctctaag gggtcagatg
    12841 tttacttcaa atatttgaaa ttacaagata aagtggtgac taaggaagag tatgaaaaag
    12901 ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg
    12961 caagtcatgt gaagtaaata atttctaata gaactttctc tccatgagga gaaggttctt
    13021 ttgggatttt taaaggaaat aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat
    13081 tggtttcgat tttcttagta acgactttga cctacacgat tatctatacc ttggttcctc
    13141 gaaaattgat tttcaagcag gatcctaact ataataaaat tgcgacaacg gctgataaac
    13201 gtgataacta tgaaaatact gtgtttgagc gtatgggcta cattgagtat tacgatacta
    13261 aagagttgca agaaaaggca agtagcatgg attcttctgt aacagtagaa gcaaatgcga
    13321 ccaataaagc tatttatgaa aagtacatca atcaattagg tcatggttgg actttgggag
    13381 aatttactga aagtggtcaa ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt
    13441 ttcacttcta tgctaacttg attgacattg accatacaaa taaaatccaa gaccctgaaa
    13501 atccagactt gaaacgctac cttcgttttg aaaatgatcc agctatcgga tggtcattgg
    13561 tcggttcagg aactaaacat aaatatctct tgtactttaa cagtcagttc ccatttgttc
    13621 atcaaaactt tgtgaacttg aatttaggtg actcttaccc aacctatgct aatacaccag
    13681 ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa
    13741 caggtaagaa aacgtcttct gtaaatattt actcaagaac ctacaagtca cctagtcagg
    13801 ctgactctcg tgaagtagct agctatggga aagatgatcc ttatacagcg actgaaagta
    13861 attaccaata tccatctatg attgtcagct ctgctattac tggtttgatt ggtttggttc
    13921 ttgcctatgc tcttgccgtg ccacttggtt cagccatggc tcgtttcaag aacacttgga
    13981 ttgatagcct ctcaacaggg gctttgacct tcttgcttgc tcttccaacg attgccttgg
    14041 tttacatcgt tcgattgatt ggatcatcta ttgcccttcc agattcattc cctatcttgg
    14101 gagctggaga ttggcgttct tacgttttac cagcagtcat ccttggtttg ttgggtgctc
    14161 ctggtacagc catttggatt cgtcgttaca tgattgactt gcaatctcaa gactttgttc
    14221 gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa
    14281 atgccatggt tccgctggtt tcaggaattc ctggtgccat cattggggtt atcggtggtg
    14341 caacccttac tgaaacagtc ttcgccttcc caggtatggg taaaatgttg attgactctg
    14401 taaaagcatc taataactct atggtcgttg gtcttgtctt catctttaca tgtatttcta
    14461 tcttctcacg tcttttggga gatatttgga tgactattat tgacccacgt attaaattga
    14521 ctgagaaagg aggcaaataa tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg
    14581 tgacgatttt gcctctgaaa ctattgatgc gccagcatat tcttactgga aatcagtgtt
    14641 taaacaattt atgaagaaaa aatcaactgt agtcatgttg ggaatcttgg tagccatcat
    14701 tttgataagt ttcatctacc caatgttttc taagtttgat ttcaatgatg tcagcaaggt
    14761 aaacgacttt agtattcgtt atatcaagcc aaatgcggag cattggttcg gtactgacag
    14821 taacggtaaa tcgctctttg acggtgtctg gttcggagct cgtaactcca tcctcatttc
    14881 tgtgattgcg acagtgatta acttggttat cggtgttttt gtcggtggta tttggggtat
    14941 ttcaaaatca gttgaccgtg tcatgatgga agtttacaac gtcatctcaa acatcccacc
    15001 tcttttgatt gttattgtct tgacttactc aatcggagct ggattctgga atctgatttt
    15061 tgccatgagc gtaacaacat ggattggtat cgccttcatg atccgtgtgc aaatcttgcg
    15121 ctatcgtgac ttggaataca acttggcgtc acgtactttg ggaacaccaa ccttgaagat
    15181 tgttgccaaa aatatcatgc ctcaattggt atctgttatt gtgacaacca tgactcaaat
    15241 gcttccaagc tttatctcat acgaagcctt cttgtctttc ttcggtcttg gattaccgat
    15301 tacagtgcca agtttgggtc gtttgatttc ggattattca caaaacgtaa caaccaatgc
    15361 ttacttgttc tggattccat tgacaaccct tgtcttggta tccttgtccc ttttcgtagt
    15421 tggtcaaaac ttagcggatg ctagtgatcc acgtacacat agataggagt agaaatgaca
    15481 aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg tggaatttga cgttcgtgac
    15541 aaagtattga cagccattcg cggcgtttcc cttgaactag ttgaaggaga agtattagcc
    15601 ttggtaggtg agtcaggatc aggtaaatct gttttgacaa agaccttcac aggtatgctc
    15661 gaagaaaatg gtcgcattgc ccaaggtagt attgactacc gtggtcagga tttgacagct
    15721 ttatcttctc acaaggattg ggaacaaatt cgtggtgcta agattgcgac tatcttccag
    15781 gacccaatga ctagtttgga ccccattaaa acaattggta gtcagattac agaagttatt
    15841 gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat tggccattga ctacatgaat
    15901 aaggttggca ttccagacgc agatagacgt tttaatgaat acccattcca atattctgga
    15961 ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct gccgacctga tgtcttgatc
    16021 tgtgatgagc caacaactgc cttggatgta actattcaag ctcagattat tgatttgcta
    16081 aaatctttac aaaacgagta tcatttcaca acaatcttta ttacccacga ccttggtgtg
    16141 gtggcaagta ttgcggataa ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga
    16201 acggttgagg aagtcttcta tgaccctcgc catccatata catggagtct cttgtctagc
    16261 ttgcctcagc ttgctgatga taaaggggat ctttactcaa tcccaggaac acctccgtca
    16321 ctttatactg acctgaaagg ggatgctttt gccttgcgtt ctgactacgc aatgcagatt
    16381 gacttcgaac aaaaagctcc tcaattctca gtatcagaga cacattgggc taaaacttgg
    16441 cttcttcatg aggatgctcc gaaagtagaa aaaccagctg tgattgcaaa tctccatgat
    16501 aagatccgtg aaaaaatggg atttgcccat ctggctgact aggaggaagg aaatgtctga
    16561 aaaattagta gaaatcaaag atttagaaat ttccttcggt gaaggaagta agaagtttgt
    16621 cgcggttaaa aatgctaact tctttatcaa caagggagaa actttctcgc ttgtaggtga
    16681 gtccggtagt gggaaaacaa ctattggtcg tgctatcatc ggtctaaatg atacaagtaa
    16741 tggagatatc atttttgatg gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc
    16801 ggaattgatt cgtcgaatcc agatgatttt ccaagaccct gccgcaagtt tgaatgaacg
    16861 tgcgactgtt gattatatta tttctgaagg tctttacaat caccgtttat ttaaggatga
    16921 agaagaacgt aaagagaaag ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca
    16981 cttgactcgt taccctcatg aattctcagg cggtcaacgt caacgtatcg gtattgcccg
    17041 tgccttggtc atgcaaccag actttgttat tgcagatgag ccaatttcag ccttggacgt
    17101 ttctgtacgt gcccaagtct tgaacttgct caaaaaattc caaaaagagc tcggcctgac
    17161 ctatctcttc atcgcccatg acttgtcggt tgttcgcttt atttcagatc gtatcgcagt
    17221 tatttacaag ggtgttattg tagaggttgc agaaacagaa gaattgttta acaatccaat
    17281 tcacccatat actcaagcct tgctttcagc ggtaccaatc ccagatccaa tcttggaacg
    17341 taagaaggtc ttgaaggttt acgatccaag tcaacacgac tatgagactg ataagccatc
    17401 tatggtagaa atccgtccag gtcactatgt ttgggcgaac caagccgaat tggcacgtta
    17461 tcaaaaagga ctaaactagt aatggtttta taatttccat gtcaattttt atggaaatta
    17521 ggctctttgt caactgtagt gggttgaaga aaagctaagc tcgagaaagg acaaatttcg
    17581 tcctttcttt tttgatgttc agagcgatga aaatccgttt tttgaagttt tcaaagttcc
    17641 gaaaaccaaa ggcattgcgc ttgataagtt tgatgagatt attggtcgct tccagtttgg
    17701 cattagaata gtgtagttga agggcgttga caatcttttc tttatctttg aggaaggttt
    17761 taaagacagt ctgaaaaata ggatgaacct gcttaagatt gtcctcaata agcccgaaaa
    17821 atttctctgg ttccttattc tggaagtgaa aaagcaagag ctgatagagc tgatagtggt
    17881 gtttcaagtc ttccgaata
//
LOCUS       DAVDWM010000044        16534 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVDWM010000044 DAVDWM010000000
VERSION     DAVDWM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.044"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(197..655)
                     /locus_tag="VUW49_001689"
     CDS             complement(197..655)
                     /locus_tag="VUW49_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET7981579.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(652..1092)
                     /locus_tag="VUW49_001690"
     CDS             complement(652..1092)
                     /locus_tag="VUW49_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981580.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1448..3397
                     /gene="mutL"
                     /locus_tag="VUW49_001691"
     CDS             1448..3397
                     /gene="mutL"
                     /locus_tag="VUW49_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7981581.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGYGISHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIAIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3722..4189)
                     /locus_tag="VUW49_001692"
     CDS             complement(3722..4189)
                     /locus_tag="VUW49_001692"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7981582.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4190..5395)
                     /locus_tag="VUW49_001693"
     CDS             complement(4190..5395)
                     /locus_tag="VUW49_001693"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7981583.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHQLALIMGNIENEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5415..6050)
                     /gene="ribE"
                     /locus_tag="VUW49_001694"
     CDS             complement(5415..6050)
                     /gene="ribE"
                     /locus_tag="VUW49_001694"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7981584.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNRF"
     gene            complement(6035..7135)
                     /gene="ribD"
                     /locus_tag="VUW49_001695"
     CDS             complement(6035..7135)
                     /gene="ribD"
                     /locus_tag="VUW49_001695"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET7981585.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7270..7384)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7541..8134
                     /gene="ruvA"
                     /locus_tag="VUW49_001696"
     CDS             7541..8134
                     /gene="ruvA"
                     /locus_tag="VUW49_001696"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7981586.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8144..8707
                     /locus_tag="VUW49_001697"
     CDS             8144..8707
                     /locus_tag="VUW49_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET7981587.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPVKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8704..9381
                     /locus_tag="VUW49_001698"
                     /pseudo
     CDS             8704..9381
                     /locus_tag="VUW49_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(9536..10567)
                     /locus_tag="VUW49_001699"
     CDS             complement(9536..10567)
                     /locus_tag="VUW49_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET7981588.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10822..11505)
                     /locus_tag="VUW49_001700"
     CDS             complement(10822..11505)
                     /locus_tag="VUW49_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET7981589.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11517..12461)
                     /locus_tag="VUW49_001701"
     CDS             complement(11517..12461)
                     /locus_tag="VUW49_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET7981590.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12593..15424
                     /gene="uvrA"
                     /locus_tag="VUW49_001702"
     CDS             12593..15424
                     /gene="uvrA"
                     /locus_tag="VUW49_001702"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7981591.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15417..16478
                     /locus_tag="VUW49_001703"
     CDS             15417..16478
                     /locus_tag="VUW49_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET7981592.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
BASE COUNT         4660 a         3287 c         3535 g         5052 t
ORIGIN      
        1 aaaatctgaa ataactgtta tgatatagca gattgaaact agaatagtac acctttgctt
       61 ctaaaacatt gttagaaatc gatttgactg tcctgatcga tttatcctgt tcttatttca
      121 tttcactata gtaaggtgca gcaggataaa gaaaaaccga gaagtttctt tctcggattt
      181 gcttattatg aggttcctat tttctatgct ccaagagttg attgatgtga tctacttttt
      241 tagcctctct tttatagaga atagcccaga tgatgagata gacaatcgca aactcgataa
      301 tgagctggag gtagaaaatc cagtggaaag ggaaccaacc agcaagagtt gctagtggga
      361 caaagccagc cagcataagg aagaaatgag tcagagtggc acgaagcatg ctccagtcac
      421 ggctaaataa gcggttgcca aagttgaaga gcataccgat ggttgcccag ataagtgtgc
      481 agtagagcaa gaccagggca ccgtgaacct gatgctgatc catcacttgg cctatgagag
      541 agtagggatt tagtggtgcg taggtatttg gtgcataaat gagtgaaaag aggatagaga
      601 ggatgaggcc gataagaaca ccggtagctg catcgtgaaa tacttgtttt ttcatagttc
      661 taatttctcc ttgatggttt ttagataacg gcgtgaagag taggtgaagc tttcgttttt
      721 caagaaaatt tctaccagac cgtttggcgt gagcttgagg tgagagatgg aatcgatatt
      781 gatgatttct gattgggaaa tttggataaa attggttggc aagagtttaa gaacctgata
      841 gagtcgcaaa tcaatgctgt aggtctgact cgcggtttct gctagaacct tccgattctc
      901 gatatagaag cgctgaatct tgccaatctc aactagatag acctgatcat cgatttttcc
      961 tttgattttt tctctttggt ccagattttc tgcgaactcg atgactttct ggactttttc
     1021 tgtcggctga ggtgcttgga caatcagctt ttcctcctcg taagtctcac taatctgtag
     1081 ttctactttc ataaatttct ctccttgttt ttcatacaaa attaatctca cttcttgtac
     1141 acctttatta atactcttca aaaatctctt caaaccacgt cagcgtcgtc ttgccgtagg
     1201 atagttgttg actttgtcag ttttatctac aacctcaaaa cagtgttttg agctgtctgc
     1261 gtctagcttt ctagtttgtt ctttgatttt tattgagtat accactattt tactccctct
     1321 ggcaagggac tttgtctatg tggagggatt gggctcctat gtggtggagc ttttctgttc
     1381 tttctgaaat atggtataat agcactaatc aatttctagg aaaatagata cagaaagggg
     1441 ctgaaagatg tctcatatta ttgaattgcc agagatgctg gcaaaccaaa tcgcggctgg
     1501 agaggtcatt gaacgtcctg ccagtgtggt caaagagttg gtagaaaatg ccattgacgc
     1561 gggctctagt cagattatca ttgagattga ggaagctggt ctcaagaagg ttcaaatcac
     1621 agataacggt tatggaattt cccacgatga ggtggagttg gccctgcgtc gccatgcgac
     1681 cagtaagata aaaaatcaag cagatctctt tcggattcgg acgcttggtt ttcgtggtga
     1741 agccttgcct tctattgcgt ctgttagtgt cttgactctg ttaacggcgg tggatggtgc
     1801 tagtcatgga accaagttag tcgcgcgtgg gggtgaagtt gaggaagtca tcccagcgac
     1861 tagtcctgtg ggaaccaagg tttgtgtgga ggatctcttt ttcaacacgc ctgcccgtct
     1921 caagtatatg aagagccagc aagcggagtt gtctcatatc attgatattg tcaaccgtct
     1981 gggcttggcc catcctgaga tttcttttag cttgattagt gatggcaagg aaatgacgcg
     2041 gacagcaggg actggtcaat tgcgccaagc aatcgcaggg atttacggtt tggtcagtgc
     2101 caagaagatg attgctattg aaaactctga cttagatttc gaaatttctg gctttgtgtc
     2161 cttgcctgag ttgactcggg ctaaccgcaa ttatatcagc ctcttcatca atggccgtta
     2221 tatcaagaac ttcctgctca atcgtgctat tttggatggt ttcggaagca agcttatggt
     2281 tggacgtttt ccactggctg tcattcacat ccatatcgac ccttatctag cggatgtcaa
     2341 tgtgcatcca actaagcaag aggtgcggat ttccaaggaa aaagaactga tgactctggt
     2401 ttcagaagct attgcaaata gtctcaagga acaaaccttg attccagatg ccttggaaaa
     2461 tcttgccaaa tcgaccgtgc gcaatcgtga gaaggtggag caaactattc tcccactcaa
     2521 agaaaatacg ctctactatg agaaaactga gccgtcaaga cctagtcaaa ctgaagtagc
     2581 tgattatcag gtagaattga ctgatgaagg gcaggatttg accctgtttg ccaaggaaac
     2641 cttggaccga ttgaccaagc cagcaaaact gcattttgca gagagaaagc ctgctaacta
     2701 cgaccagcta gaccatccag agttagatct tgctagcatc gataaggctt atgacaaact
     2761 ggagcgagaa gaagcatcca gcttcccaga gttggagttt ttcggacaaa tgcacgggac
     2821 ttatctcttt gcccaagggc gagatggact ttacatcata gatcagcacg ctgctcagga
     2881 acgggtcaag tacgaggagt accgtgaaag cattggcaat gttgaccaaa gccagcagca
     2941 actcctagtg ccctatatct ttgaatttcc tgcggatgat gccctgcgtc tcaaggaaag
     3001 aatgcctctc ttagaggaag tgggcgtctt tctagcagag tacggagaaa atcaatttat
     3061 tctacgtgaa catcctattt ggatggcaga agaagagatt gaatcaggca tctatgagat
     3121 gtgcgacatg ctccttttga ccaaggaagt ttctatcaag aaataccgag cagagctggc
     3181 tatcatgatg tcttgcaagc gatctatcaa ggctaatcat cgtattgatg atcattcagc
     3241 tagacaactc ctctatcagc tttctcaatg tgacaatccc tataactgtc ctcacggacg
     3301 tcctgttttg gtgcatttta ccaagtcgga tatggaaaag atgttccgac gtattcagga
     3361 aaatcacacc agtctccgtg agttggggaa atattaaaag tataaaaaag tctgggaaaa
     3421 attttcaaaa tcaaaaaaac gcataaaatc aggtgttcaa aaaccttgat tttatgcgtt
     3481 ttatcatgga aatagttact tcattttttc ctaattcttt tcgaaactct ttttaaacga
     3541 cgtcagtttt atcagtaatc tcaaaacagt gttttgagct aattttgcca gttttgtctg
     3601 taacatcgaa gttgtgtttt accactctgc gactggtttc ctagtttgct ctatgatttt
     3661 cacagagtat taaattgcga ttttgccaag tttctttatt cgtctaaaag tagagtctgt
     3721 tctatgcgtc taatgtacga atcaggttga ccatttcaat agctccttgt gcacactcag
     3781 aacccttatt tcctgcttta gtaccagctc gttctatggc ttgttcaatt gtatctgtcg
     3841 ttagcacacc aaacataaca ggaatttcgc tatttaaact gatttgggcg attcccttag
     3901 atacctcgct acatacataa tcataatgac ttgtattccc tctaatgaca gctcccaagc
     3961 agataattgc atcatatttt ttactttttg ccatttttga tgcaatcagt ggtatttcaa
     4021 aagctcctgg aacccaggct acctcgatat ctttctcgtt tacattctct cttttgagat
     4081 tatctagtgc tccagataat aattttgaag ttataaattc attaaatctc gctacaacaa
     4141 tacctatttt aatattgttt gctactaaat taccttcata agtgttcatt tatttttcct
     4201 ccatatttaa aatgtgaccc attcgatttt tctttgtttc taaataaaaa ctatcgtaag
     4261 gattggcttc tatttcgatt gatattctac tggaaatggt aattccatat ttttctaact
     4321 gttcaacctt gtcaggatta tttgtcagta aatgaagtga ctgaagtccc agatctttaa
     4381 gcatttgtgc tccaatatga tattctctta aatcaccttc aaagcctaat gcaagattgg
     4441 catcaagcgt atccatgcct tgatcttgta aatgataggc ttttaattta ttgataagtc
     4501 caattcctcg tccctcctgt cgcaagtaaa gtaagacacc cgaaccattc tcaacaatca
     4561 ttttcatagc tttatcgaat tgctgtccac aatcgcaacg taaggagcct aaaacatccc
     4621 ctgttaaaca ttcggagtgg acccgacata atacattggc ttcattctct atgtttccca
     4681 taataagagc aagttgatgt tccccattta gtttatctat atagctaatt gctttgaaat
     4741 taccgtatct agtaggcata ttgacagttg aaactcgttc taccagctga tcatatactt
     4801 ttctatattc ttgtaattct ttgatggtaa ttagtggaat gttgtgtttc ttcgagaact
     4861 gaattaaatc atctgttctc atcattttgc catcatgatt cattatttca caacataggc
     4921 cacactcttt tagtccagct aattttaata aatcaacagt tgcttctgtg tgtccacttc
     4981 tttctagaac accacctttt tttgcaatta aaggaaacat gtgtcctggc ctgcgaaaat
     5041 cagagggtgt tatatcttca gctacacaca tacgtgcggt cagtcctctt tcctcggcag
     5101 aaatacctgt ggtcgtttct ttataatcaa ttgaaactgt aaaagcagtc ttatgattat
     5161 ctgtattgtt ttcaaccata ggtgaaagca ttaattgatt agctaaactt tcgctcatag
     5221 gcatacaaat taatcctttg gcataagtag ccataaaatt aacattttct gttgtagctg
     5281 cttgtgcaga acaaattaag tctccttcat tttctctatc cttgtcgtct ataacaagaa
     5341 caagtcgtcc cttctgcaat gcttctaatg cttcttgtat ttttcgatat tccattgact
     5401 gattatcctt tctgctaaaa tctattttga tataatagtt ccttagatat ttctgatttt
     5461 ggagagttat ccatcagttt ttgcacatat ttacctaaga tatcattttc aagatttact
     5521 gtactcccga cttgtttact cttaagaatg gtttgttcca aggtatgagg gataacagat
     5581 actgaaaagt ttactttgga gactttagcg acagtcagac taatgccgtc aattgtaata
     5641 gatccttttt caactattaa atctaaaatt tctttttgtg tgttgatttg ataccataca
     5701 gcattatcat ctttttttat tgacgagatt tttcctgtac catcaatgtg tcctgtaacg
     5761 acgtgacccc caagtcgacc gttgacagat aaggctcttt ctagattcac ctcacttcca
     5821 tgttttaata gagtaagagc tgttcgactc catgtttcat tcattacatc aactgtaaag
     5881 gattgatgat tgaaatgagt aactgtaaga cagataccat ttactgctat actatcgcct
     5941 aaatggatat ccgttaatat ttttgaggct ttaattgata gtttacaatt acgagagtct
     6001 ttctgtattc tttcaacttt tccgatttct tcaattattc ctgtgaacat ggataaatca
     6061 cttcactttc tatgagatag tcatttccta tttgagaaaa tgcataaggt ttcaatctaa
     6121 tagcgtcatt tggcaaagaa ataccttcac ctccgacagg aaacttggca ctacctccaa
     6181 aaatttttgg tgcaatatat attttcagct catcaacaat ttgttgttcc aaagcactcc
     6241 aattcattag actgccccct tctagaacta ggctatcaat ctgcatgttt cctagatgtt
     6301 gcattaaact cgataagtct atatgattgc cttttttctt tatggaaagt atttcacagc
     6361 catgattttg atatagcttc attttatttt tgtcttcaga ggaagtggca atgtaagttt
     6421 taatatcatt tgctgttttt acgattttag aggtaagagg agttcgtaaa tgtgtatcgc
     6481 atatgatacg gataggattt ttcccttcct ccaatctaca tgtcagcaaa ggatcgtctt
     6541 gaataacagt attgactccc accataattg cactaacata gtgtcgtaac tgatgcacat
     6601 gctttcttgc ttcttcttca gtaatccatt tggattgatt tgttttagtg gctatttttc
     6661 catccattga cattgcatat ttcataaaaa catagggtac atgctgggta atatactttc
     6721 taaaactttt tattaagtta agacactcat tttctaaaat tccaacagta acttgaagat
     6781 tattttcctc aagtatcttt actccttttc cagatacaat aggattacag tctaggcttc
     6841 caatgactac tcttgtaata ccactatcga ttatagcatc tatacaggga ggtgttttcc
     6901 cgaagtgaca acagggttca agtgttacat aaagcgtcgc tccgacaggg gattttctac
     6961 agtttttaag agcatttctc tcagcatgtg ggccaccaaa aaactcatga taaccttgtc
     7021 cgatgatgtg attatctttt acaataactg cgccgaccat aggattggga ttgacgtaac
     7081 cagccccttt ttgtgccagt tttattgcta atttcatata ttttgaatcg ctcatctcac
     7141 tacctccaaa aaaaatatac cttgaatagg ggactactca aggcatacaa aagaaaactt
     7201 atgcgattaa caaaaatact ctgaaatgac aagtaatcat ttcagagcac gcaaaaagca
     7261 caaatatact tttatcttct ttcatccaga ctatactgtc ggctttggaa tttcaccaaa
     7321 tcatgccttt cggctcgtgg gctataccac cggtagggaa ttgcaccctg ccctgaagat
     7381 agttattcaa ttacagatga ttatagtact taattttgaa tatgtcaaca gataaatacc
     7441 gattgttttt gatatactgt atttgtgata atcgattctc gctcctcgga taaagaaaat
     7501 atgatatact agataaacga aataagagag aaggaatact atgtacgcat atttaaaagg
     7561 aatcattacc aaaattactg ccaaatacat tgttcttgaa accaatggta ttggttatat
     7621 cctgcatgtg gccaatcctt atgcctattc aggtcaggtt aatcaggagg ctcagattta
     7681 tgtgcatcag gttgtgcgtg aggacgccca tttgctttat ggatttcgct cagaggatga
     7741 gaaaaagctc tttcttagtc tgatttcggt ctctgggatt ggtcctgtat cagctcttgc
     7801 tattatcgct gctgatgaca atgctggctt ggttcaagcc attgaaacca agaacatcac
     7861 ctacttgacc aagttcccta aaattggcaa gaaaacagcc cagcagatgg tgctggactt
     7921 ggaaggcaag gtagtagttg caggagatgg ccttcctgcc aaggtcgcag tgcaagcaag
     7981 tgctgaaaac caagaattgg aagaagctat ggaagccatg ttggctctgg gctacaaggc
     8041 aacagagctc aagaaaatca agaaattctt tgaaggaacg acagatacag ctgagaacta
     8101 tatcaagtcg gcccttaaaa tgttggtcaa ataggagcag agaatgacaa aacgttgttc
     8161 gtgggtcaag atgaccaacc cactctacat cgcctatcat gatgaggagt ggggccagcc
     8221 cctccatgat gaccaagcgt tgtttgagtt gttgtgtatg gaaacctatc aggcaggcct
     8281 gtcttgggaa acggtactca acaaacgcca agctttccga gaagcctttc atagctatca
     8341 aattcactca gtcgcagaga tgactgacac tgaattggaa gccatgctgg agaatccagc
     8401 tatcattcga aatagagcca agatttttgc tacacgcgct aacgcccaag cttttctaca
     8461 gttacaggca gagtacggct cttttgatgc ctatctttgg tcttttgttg aggggaaaac
     8521 tgtcgttaac gatgttcctg attatcgcca agccccagtt aaaacaccct tatctgagaa
     8581 attagccaaa gatctcaaaa aacgaggctt caagttcaca ggcccagtcg ccgtattgtc
     8641 ttttctacag gctgcagggc tagttgatga ccacgagaat gattgtgagt ggaaaggtct
     8701 taaatgatgt ctaacaaaaa taaggaaatt ctgatttttg cgattctcta tacagtcctc
     8761 tttgtgtttg atggcgttaa attgctggct tctttaatgc catctgccat tgcaaattat
     8821 cttgtttatg tagttttagc tttatatggc tccttcttgt tcaaggatag attgatccaa
     8881 taatggaagg agattagaaa gactaaaaga aaattcttct ttggcgtctt aacaggatgg
     8941 ctctttctca ttctgatgac tgttgtcttt gaatttgtat cagagatgtt gaagcagttt
     9001 gtgagactag atggacaagg tctaaatcag tctaatattc aaagtacctt tcaagaataa
     9061 ccactactga tagctgtttt tgcttgtgtc attggacctc tggtagaaga attatttttc
     9121 cgtcaggtct tattgcatta cttgcaggaa cggttgccag gtttactaag cattattctg
     9181 gtaggacttg tttttgcttt gactcatatg cacagtttgg ctctatcaga gtggattggt
     9241 gcagttggtt acttaggtgg aggccttgcc ttttctatta tttatgtgaa agaaaaagag
     9301 aatatctact atcccctact tgttcacatg ttaagcaaca gcctctcctt aatcatttta
     9361 gctatcagta tagtaaaatg aaataagaac aggacaaatc aatcaggaca gtcaaatcga
     9421 tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaataca ctgtaacatg
     9481 tgatgaaaat gccagtaatg ataccgagaa aaaagctgag aaacttttcc cagctttatt
     9541 tgttatagtc aaagcgaatg acttgttcct gtgcatctac atgagcatgg accccaaagg
     9601 gtacaattgc tcttggagtt gcgtggccga cattcagatt atagacaatc gggatattgc
     9661 tgtcaatgat atctaatagt gcctctttat agtcgtcatg gaaagtttca tccataggtt
     9721 ttccgaccaa gagtccattg atgaccgcga atatgccagt gtcctttaaa gttagcaaca
     9781 tctttttgaa gtcttctggc ttaggctttt cttcgcttgt ttcgagcaag aggattttcc
     9841 cttcccagtc tgacaagtca gggaaaagtt tgtatttttg gcagagttcc gtgctatctg
     9901 cgtatcgaga gttgtcaaag atatcgtaga gggattctag gcaaccaccg aggattttcc
     9961 cctcgaactg agcacttcct tgcaacaagt caaaacctgt atttgtatga ctgacacgag
    10021 gtgttcccag ggccgtggga ctaaaatcag ttcgttcctc ataccaaacg tcactagggc
    10081 ggatttctga aattcttccc gtctcaatca attctttaaa gtagtgaagg ctataggcta
    10141 gcatttcttt gtctaattca caaatgtctg ctaaaaagga ttgaccataa aaagtcttga
    10201 ttcctaattt atgcaacatg aggtggttca tggttgtatc cgagaagcca agaaaaattt
    10261 tttgcttgat aaccttttgg agttggtcat tttcaaaaag ataaggtagc aagcgatagg
    10321 tatcatctcc accgatggca cataggatca tgtcgatgct atcatcagaa aaggcatgaa
    10381 tcaaatcctc tgcacgagct tcaggatggt ccttgataaa gtctaatcct tttaacgaat
    10441 ggggcaaaaa gatgggattg agtcccagat ccttgagacg ttggataccc aagtccactt
    10501 cgtgtttgac aaaatcctct ccgataatgc cactagataa actaacaata ccaatagtag
    10561 aaaccatatc tcatcctcct agaaatagat tgagcctatt ttatcacaaa atataagaga
    10621 aagctatgtg ggatttcaga agaatacttt taatactctt cgaaaatctc ttcaaaccac
    10681 gtcagtttta tctgcaacct caaagctgtg ctttgagcaa cctgcagcta gcttcctagt
    10741 ttgctctttg attttcattg aatataactc aaaaaagtaa gtgaaaaagc aaccgcacct
    10801 gcagttgctt ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac ttgcactacg
    10861 gatattcaag cgtttcttga gatagaagcg aagggctagg agggctgctc caataatagc
    10921 tagtggcaat ggatccagta ctgggttaag gctagctggt aggaagcttg ttgcaaagaa
    10981 gacaagcaac caaaggaaca tagaagctag gataactagt acagatttcc agaaaggtgg
    11041 acgttgactg cgatccatat ctggtccata gtattggtaa acaaagtagt acatcaagta
    11101 gaaggcaaat ccaccaacca gtccaactaa tagaagagta atcaatccat agccgaaagc
    11161 ttggtctgcc gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc tagtgatgaa
    11221 aagagctgag tccataatca tcagttttgg gtcatcattt tcttttggat gctctttttc
    11281 gtactgctct ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt atagggaacg
    11341 ggcagttgta cctttagatt gctcctcaag gatttgggga atgacttcct caaaaatagt
    11401 ctggatttca gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa tgtggacaaa
    11461 ctcttggttt ttcttagtta atttttgtag atcaatttga gacataggaa ctcctcttag
    11521 aaccattttt tatggatgag atagagagtg agcgagacac tcatagcaaa ggcgataaag
    11581 acgattaacc agaaggcatt tggctctccg tttaggggga tttcattatc cttaaagttc
    11641 atcccgtagg cagaaaagac catggttggg atggacatga cgatggtcac aagggccaag
    11701 gttttcatga tgttgttctg gttgttagaa atgatagagg caaaggtctc tgtcatagaa
    11761 tgcaagacgt ttccataaat atctgccatc tcgatggcct gttgggtttc aatcagggtg
    11821 tcttcaagca ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga actggtcaat
    11881 ttcttaatca cgcgctcatt tgttttgagg gaggccttga aatagacgat agttttttcc
    11941 aattccatga gctcaatcaa ttcttcatta cgagttgatt gatgcagttg actttcgatt
    12001 tgttcactct tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc attgcgataa
    12061 agaatttgaa agataaaacg tgaacgcatg aaggtataga aattacgcaa tcgacggttg
    12121 ataaagacat caaggacagg tagtggttcc aaacacgtag tgataatggt ttcctcagtg
    12181 atgataatac caagcgggat ggttacgtag taggtgcggt tatttctttc ctccgtgacc
    12241 ggcacgtcta cgataatcag ggtatactcg tcttcaatgg taatacgaga catttcttcc
    12301 gcatcgagcg gtgctcgaag gtcagcaata tcaatatcga aggtgttggc gatttcgagt
    12361 gattcatttt gagtcggatt gacgagattg atccaagtac ccggttcaag cgtatcgatc
    12421 tctttaaatt cagttgttgt agagagaaaa acttgtttca tatcccttat cctttctcat
    12481 ttttcagatt ttttcacact gtactattat actacaaaat cggccttttg ggtaatagaa
    12541 tctcactttt tttggtataa tggtaagcaa taatggacta gaaagaacaa agatgcaaga
    12601 taaaattgtc attcatgggg cgcgtgccca taatttaaaa aatattgatg tggagattcc
    12661 gcgagacaag ttggttgtcg tgactggctt gtcaggttca gggaagtcca gtctggcttt
    12721 tgatactctc tatgcggagg gacaacgtcg ctatgtagag agtttgtccg cctacgctcg
    12781 tcagttcttg ggaaatatgg aaaaaccaga tgtagatgcc attgatggtc tcagcccagc
    12841 tatttccatc gaccagaaaa cgactagtaa aaatcctcgc tcgacggtgg gaacgacgac
    12901 tgaaatcaat gactatctgc gtctcctcta cgcacgtgtg gggacgcctt actgtatcaa
    12961 cggacatgga gctatcaatg cttcttctgt ggagcaaatc gttgataagg ttttggagtt
    13021 acctgaacgc cagcgcttgc agatcttggc tcctgtcatc cgcaagaaaa aaggccaaca
    13081 taagagtgtt atcgagaagg ttcagaaaga cgggtatgtt cgtgtccgtg tggatgggga
    13141 agtctatgat gtgaccgaag tgccagagtt gtctaagagc aagcaacaca atatcgatgt
    13201 cgtggttgac cgtattgtta tcaaggaggg cattcgtagt cgtctctttg attccattga
    13261 ggctgccctt cgtattgcag aaggttatgt cattatcgac accatggacg actcggagtt
    13321 gttgttctct gagcattatg cctgtccagt ttgtggtttt actgtcccag agttagagcc
    13381 tcgtctcttt tctttcaatg ctccttttgg ctcttgtagt gagtgtgacg gcttgggcat
    13441 caagctggag gtggatactg atttggtagt gccagatgct agcaaaacct tacgtgaggg
    13501 agcgctagca ccttggaatc ctatctcatc caactactat ccaaacatgt tagagcaggc
    13561 catgaaagtc tttggagtgg ctatggataa gccttttgag gacctgtcag aagaagataa
    13621 gaacttgatt ctctatgggt cagatggtaa ggaattccat ttccactatg agaatgaatt
    13681 tggtggcgtg cgcgatatcg acattccgtt tgagggagtt atcaataata tcaaacgtcg
    13741 ttaccatgaa accaatagcg attacactcg cactcagatg cgtctctaca tgaatgagct
    13801 gacctgcgga acctgtcaag gctatcgtct caatgaccag gccttgtctg tccgtgtggg
    13861 cggccagcaa gggccacata ttggagaaat ctcagacctg tctatcgctg accacttgga
    13921 cttggtgagc cagttaactt tgtctgaaaa tgaagccatc attgctcgtc ccattctcaa
    13981 ggaaatcaag gatcgtttga ccttccttaa taatgtgggt cttaactatc taaccctgtc
    14041 acgttcagca ggaacccttt caggtgggga aagtcagcgc attcgtttgg caacccagat
    14101 tggttccaac ctatcaggtg tcctctatat cctagacgag ccgtcaatcg gtcttcacca
    14161 gagggacaat gatcgcctga ttgccagtct gaaaaagatg cgtgacttgg gcaatactct
    14221 tatcgtggtg gaacacgacg aagataccat gcgtgaggct gattatctga ttgacgttgg
    14281 tcctggtgcc ggtgtttttg gtggtgagat tgttgcagca ggtacaccta agcaagtggc
    14341 tcgcaacagc aagtctatca caggtcagta cttgtcaggc aaacgtgtca tcccagtacc
    14401 agaagagcgc cgtgtcggaa atggtcgttt tattgaagtg ataggagcgc gtgagaacaa
    14461 cttgcaaaat gtcacggctc gctttccact aggaaaattt atcgcagtga caggtgtgtc
    14521 gggttcaggg aaatcgactc taatcaacag cattctcaaa aaagccattg cccagaagct
    14581 caaccgtaat tcagacaaac ctggtaagtt taagactatt acagggattg agcatgtcga
    14641 ccgtttgatt gatattgacc agagccctat cggacggacg ccgaggtcta acccagctac
    14701 ctatacagga gtttttgacg atatacgtga cctttttgct cagacaaatg aagccaagat
    14761 tcgaggctac aagaaggggc gcttcagttt caacgtcaag ggaggtcgct gtgaagcttg
    14821 ctcaggtgac gggattatca agattgagat gcacttcttg ccagatgttt atgtggcttg
    14881 tgaagtttgc cacggaaccc gctataacag tgaaacccta gaagttcact acaaggaaaa
    14941 gaatatttcg caggtcttgg atatgacggt caacgatgcg gtagaatttt tccaacacat
    15001 tccgaaaatt caacgcaaac tccagaccat caaggatgta gggctaggct atgtgacctt
    15061 gggacagcca gctaccaccc tttctggggg agaagcccaa cgtatgaagt tagctagcga
    15121 actccacaaa cgatcgacag gaaaatcttt ctacattctg gatgagccga cgacagggct
    15181 tcatacagag gacattgctc gcctgctcaa ggttttagct cgctttgtag acgatggcaa
    15241 tacagtcctc gtcatcgagc acaatctgga tgttatcaag acggcagacc atatcattga
    15301 cttgggacct gagggcggtg tcggtggtgg aaccatcatc gtaacaggaa ctccagaaga
    15361 agtagctgcc aacgaagcca gctatacagg acactatttg aaaggaaagt tacatcatga
    15421 ataaacgcgt acaagcattt ctagctaaaa tgcaagaaaa agaactagat ggtatcatca
    15481 tcaacaatct taaaaacgtc tattatttga ctggtttttg gggctcaaac ggaacagtct
    15541 ttatcagtcg tgaccgtcag gtcttagtga cagactctcg ctatatcatc gcagctaagc
    15601 aagaaaccag tggttttgag attgtggctg atcgtgatga attggctgtc attgcaggaa
    15661 ttgttaagga catgggcttg actcgtatcg gttttgaaga tgagatttca gtgtcttatt
    15721 accaccgtat gcaggcagct tttgcaggtt tgaacttgct tccacaaact cagtttgtgg
    15781 aaggtcttcg tatgattaag gatgaggcag agattgcagc gattcgcaag gcttgttcta
    15841 tctcagacca agctttccgc gatgcgcttg actttatcaa accaggaaaa actgaaattg
    15901 agattgccaa cttccttgat ttccgcatgc gtgagttggg agcatctggc ttatcttttg
    15961 atacgatcct agctagcggt atcaattctt ctaaacccca tgcccatcca atgcacaaac
    16021 cagtggagtt gggagaagcc attaccatgg acttcggctg tctctatgac cactatgtca
    16081 gtgatatgac ccggactatc tatctagggc atgttagcga tgagcaggca gagatttaca
    16141 atacggttct aaaagctaac caagccttga ttgaccaagc taaggcaggc ttaggtttcc
    16201 gtgactttga caaaatccct cgtgatatta tcattgaggc aggttatggt gactacttta
    16261 ctcacggcat tggccacggt attggtctgg atatccatga ggaaccctac tttagtcaga
    16321 cttctacaga aactattaag acaggtatgg ccttgaccga tgaaccaggt atctatatcg
    16381 aaggcaaata tggcgttcgt atcgaggatg atatcctgat tacagagaca ggttgtgaat
    16441 tattgaccct agctccaaaa gagttgatag tcatttagtt gtttgtcaag aaaaaagtac
    16501 gaaaatgacg aaaaaagtca aaaaaaatta aaaa
//
LOCUS       DAVDWM010000045        15476 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVDWM010000045 DAVDWM010000000
VERSION     DAVDWM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.045"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            137..292
                     /locus_tag="VUW49_001704"
                     /pseudo
     CDS             137..292
                     /locus_tag="VUW49_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            492..3767
                     /gene="rexB"
                     /locus_tag="VUW49_001705"
     CDS             492..3767
                     /gene="rexB"
                     /locus_tag="VUW49_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET7981593.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQESQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3764..7414
                     /gene="addA"
                     /locus_tag="VUW49_001706"
     CDS             3764..7414
                     /gene="addA"
                     /locus_tag="VUW49_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET7981594.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSAQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLILE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKVDFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGRLIILKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAAFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIKNIEKYLI
                     LLGKDEVQVVKV"
     gene            7584..8762
                     /locus_tag="VUW49_001707"
     CDS             7584..8762
                     /locus_tag="VUW49_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981595.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            8971..14697
                     /locus_tag="VUW49_001708"
     CDS             8971..14697
                     /locus_tag="VUW49_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET7981596.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTKLINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEET
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSEAVVTDKG
                     EPAVQPELSEAVVTDKGEPAVQPELSEAVVTDKGEPAVQPELPEAVVSDKGEPAVQPE
                     LPEAVVTDKGETEVQPESPDTVVSDKGEPKQVAPLPEYTGPQASAIVEPEQVAPLPEY
                     TGVQAGSIVEPEKVEAPKEYTGKIEQPSAEDTKPENEASSTNGESERPKDKIKEEKQV
                     DKKLELRNVSNVELYTVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIE
                     STTKNNKEVYKIVAHAENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNS
                     DPNGTFHLGATMDAREVELPDDQESYVKNEFYGKLIGENNGKYYAIYNLKKPLFKTLN
                     TATIQNLSIKEANVSSKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNS
                     RISNSSFTGRITNTYDTTAGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVG
                     GIAGVVEKSATIKYSYVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDV
                     NVTNGNAIAGYNFNGIKTIETYSNKNNKVVNVVQEDDEVVTKDSDVQRGTVLDADKVK
                     EKKVELVSKHSTKVEDFDFTSRYNTNYNEVTGYQQSREQVYKNIEKLLPFYNRETIVK
                     YGNLVEDNSDLFTKKLLSVVPMKNNEVITDINKNKQEINKLLLHFEGNKSRVLNIAYK
                     NDFSKVAEYDIANTKLMYTPNTMLHDYNNIVKTILNDLKSVQYSSADVRKVLDISGNI
                     KLTELYLGEQFEKTKANIEDSLSKLLTADAAIVENNNKVIDNYVIEKIKNNKEALLLG
                     LTYLERWYNFNYGETNAKDLIMYHLDFFGKSNSSALDNVIELGKSGFNNLLAKNNVIT
                     YNVLLAKNYGTESLFKALEGYRKVFLPTISNNEWFKKQTKAYIVEEKSTIEEGREKQG
                     KEGTKYSIGVYDRLTNPSWKYQSMVLPLLTLPEEKTVFMIANISTIGFGAYDRYRSSE
                     YPKGEKLNKFVEDNAKEAAKRFRDHYDYWYKILDNDNKEKLYRSILVYDAFKFGTDKD
                     KDKVTHQATFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMAYRMLDKDGAVTY
                     THEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITINSVLKYEDSENS
                     TRLQVADPTQRFNSAEDLHNYMHNMFDVIYMLEYLEGKAVANLETNQKYELLRKIENK
                     FDLDQDGNNVYATNVVRRLTMDEVNKLNSFDSLIENDIITSRGYKDQEYKRNGYYTID
                     LFSPIYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYISNQYEKDAKAAGSKIN
                     SYGKEVGLVTDELVLEKVFNGQYKTWTQFKKDMYKEREKQFSKLNRVNFINPNNPLSR
                     QRNVSVTDIGVLERMIVEAVRDDAQDDVAKFYPETNSRVLKLKKAIYKAYLDQTNDFR
                     SSIFENKK"
     gene            14741..>15476
                     /locus_tag="VUW49_001709"
     CDS             14741..>15476
                     /locus_tag="VUW49_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7981597.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGEPEVQPTLPEAVVTDKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKL"
BASE COUNT         5294 a         2779 c         3178 g         4225 t
ORIGIN      
        1 ttcattttac tatagtagat tgaaataaga tacgaacagc ttgattaggg aaatcaaatt
       61 aatttctaga aatattttag catccacggt gtactattat agtttcaatt tactatagtt
      121 caatcattct atcaagaata ttgaatatat aaaggcacgt aatattaaaa cttcttctga
      181 tatcgaatta gagtccgatg ttgatggcga taaatcagac aatctaccag tcgacattaa
      241 gatactggga caacacattg aagtgtattc cgtacctaag aaacaatcat aaaactcaaa
      301 gccttgttac cgaggctttt ttattatgtg attttcagta gccagactta ttctatctaa
      361 tacaagtaaa gctaagtatt taagatttag cagatgatga agaaaagaaa ggatggccta
      421 aatattttaa cagatttacg aggctaatca tggtataata tagggtgatg aaaaattcca
      481 acgaggctga gatgaaatta ctttatactg atattcggac ttctttgaca gaaattctaa
      541 caagagaggc agaagagcta gttgcagctg gcaagcgggt cttctacatt gcccccaact
      601 ctctttcttt tgaaaaggaa cgcgccgtgc tggaatactt gtcccagcag gcttcttttt
      661 cgattaccgt cacgcgcttt gctcaaatgg ctcgctatct ggtcttgaat gatttaccag
      721 ctaaaactac tcttgatgat atcggtcttg ggttggcctt ttacaaatgc cttgccgaac
      781 tcgatcccaa ggacttgcgt gtttatggcg ctattaagca ggatcctcaa ttgatccagc
      841 agttaattga gctttaccat gagatgacca aatctcagat gagttttttg gacttggaga
      901 atttaacaga tgaggataag agggcggatt tactcttgat ttttgagaaa gtaacagcct
      961 atcttaatca aggtcagtta gcccaggaaa gtcagttgtc ccatttgatt gaggctattg
     1021 agaatgacaa ggtaagtagt gattttaatc aaatcgcctt ggtcattgac ggctttactc
     1081 gtttttctgc tgaggaagag cgggttgtgg acttacttca cggcaaaggt gttgagattg
     1141 ttatcggggc ttatgctagt aagaaagcct ataccagtcc ttttagcgag ggcaatctct
     1201 accaagccag cgtaaaattt ctccatcatc tggcttctaa ataccaaacg cctgctcagg
     1261 actgttctca aactcatgag aagatggata gttttgacaa ggcctctcgt ttgttggagt
     1321 cttcttatga cttttcagaa ctcgctttgg atgtcgatga gaaagaccgt gaaaatttac
     1381 aaatctggtc ttgtttgacg caaaaggagg agttggagct agtagcccgt agtattcgtc
     1441 agaaattaca tgagaactca gacctgagct acaagcattt tcgtattctc ttgggggatg
     1501 tagcttctta ccagttatct ctcaaaacca tttttgacca gtatcagatt cctttttatc
     1561 ttggtagaag cgaagccatg gctcatcatc ccttgactca gtttgtcgag tctattttag
     1621 ctttaaaacg ttaccgtttt cgtcaggagg atttgattaa tcttcttaga actgatttgt
     1681 atactgacct cagtcagtct gatattgatg cttttgagca atatatccgc tatcttggta
     1741 tcaatggctt gccagccttt cagcaaacct tcaccaaatc ccaccatgga aaatttaatc
     1801 ttgagcgttt gaatgtcctc cgcctgagaa ttttagcacc tcttgaaacc ctctttgcca
     1861 gccgaaaaca aaaggctgaa aaactcctac aaaaatggag tgtctttcta aaagaaggag
     1921 ctgtgaccaa gcagttacaa gatttgacaa cgactttgga agctgtagaa caggaaagac
     1981 aagccgaagt ttggaaggct ttctgccatg ttttagaaca atttgcgact gtttttgctg
     2041 gttcacaggt tagtctggaa gacttcctag ccttgctcca ttctggaatg agtttgtccc
     2101 aataccgtac cattccagca acagtggaca ctgttctggt gcagagttac gatttgattg
     2161 caccattgac tgctgacttt gtctatgcta ttggactaac tcaggacaat ttaccaaaaa
     2221 tttctcaaaa caccagtctt ctgacagatg aagaaagaca aaacctaaac caagcgaccg
     2281 aagaaggcgt tcaattactg attgccagca gtgaaaatct caagaaaaat cgctacacta
     2341 tgctttcctt ggtcaattct gctcgtaagc agttgttctt gtcggctcca agccttttta
     2401 acgaaagtga aagtaaggaa tctgcctatc ttcaagagtt gatccatttt ggatttaggc
     2461 ggagagagaa gaggatgaat cacaaaggac tgtctaagga ggatatgggg tcctatcaca
     2521 gtcttttgtc tagtctggtt gcctatcacc agcagggtga gatgagcgat actgagcaag
     2581 atttgacttt tgtcaaggtt ctgtcgcgtg tcataggtaa aaaactagat cagcaaggtc
     2641 tggaaaatcc agctatccca accagtccaa gcagcaagac cttagccaag gacaccttgc
     2701 aagctctcta tcctgccaaa caggagtttt acctgtctac gtcgggtttg acagagtttt
     2761 atcgcaatga atacagttat ttcctacgct acgttttagg cttgcaggag gaattacgtt
     2821 tgcatcctga tgcccgtagt cacgggaatt tcttgcatcg tatctttgaa cgcgccttac
     2881 agttgcctaa tgaagattcc tttgaccaac gtctagaaca agctattcaa gaaaccagtc
     2941 aagaacgcga atttgaagct atttatcaag aaagtttgga agcccagttt accaaggaag
     3001 ttttgcttga tgttgcacgg acaactggac atattctccg acacaatcca gccatcgaaa
     3061 ccatcaaaga agaagcaaat tttggtggaa aagaccaagc ctttattcaa ttagacaatg
     3121 gacgcagtgt ctttgtacga ggcaaggtgg accggattga ccgtttgaaa gctaatggag
     3181 cgataggagt agtagactac aaatccagtc tgactcagtt ccagtttcct catttcttta
     3241 atgggctcaa ttctcagtta ccaacctatc ttgctgccct aaaaagagaa ggggagcaga
     3301 actttttcgg cgccatgtac ttggaaatgg ctgaacctgt ccaatctctg atggcggtaa
     3361 aaagtctggc aggagcagtg gtagaagcca gcaaatctat gaaataccaa gggctcttct
     3421 tggaaaaaga aagcagttat ttaggcgaat tttataacaa aaacaaggct aatcaactga
     3481 cagatgagga atttcagctc ctactggact acaatgccta tctttacaag aaagctgctg
     3541 agaagatttt agcaggccgg ttcgccatca atccttatac tgaaaatggc agaagcattg
     3601 ccccatacgt ccagcaacat caggctatta caggctttga agccaattac catctgggcc
     3661 aagcccgttt cctagaaaag ttggacctag ctgatggcaa gcgtctggtc ggagaaaaac
     3721 tcaagcaagc ttggcttgaa aaaataagag aggagttgaa tcgatgaagc ttattccctt
     3781 tttaagtgag gaggagattc aaaaactgca agaagcagaa gcaaattcga gcaaggaaca
     3841 gaagaaaact gccgagcaaa tcgaagctat ctacacttct gcccagaata tcctggtctc
     3901 agcatcggct ggttctggaa agacctttgt catggcagag cgcattctgg accaattggc
     3961 gcgtggtgtc gaaatttctc aactctttat ctcaaccttt accgtcaagg ctgcaactga
     4021 acttaaagaa cgtttagaga aaaaaatcag caagaaaatc caagaaacag atgatgtcga
     4081 cctcaaacaa cacttgggtc gccagttggc agacctaccc aacgctgcca ttggaaccat
     4141 ggattctttc acacaaaaat tccttggcaa acatggttat ctgcttgata ttgcacctaa
     4201 tttccgtatt ttacaaaacc aaagcgagca acttattctc gaaaacgaag tctttcatga
     4261 ggtctttgaa gcgcattacc aaggtaaaca gaaagagacc tttagtcatt tgctgaaaaa
     4321 ctttgctggg cgtggcaagg acgaacgggg tctgcgccag caggtctata aaatctatga
     4381 cttcctccaa tccaccagta atcctcaaaa gtggctgagt gaatctttcc tcaaaggatt
     4441 tgagaaagtt gattttacca gtgaaaaaga aaaactgacc gagcaaatca aacaagccct
     4501 ttgggatttg gaaagctttt tccgttacca tctggataac gatgccaagg agtttgcaaa
     4561 ggctgcctat ttagaaaatg ttcagttaat tctggatgaa attggctccc taaatcagga
     4621 gtccgatagt caggcttatc aggcagtgct tgcgcgtgtt gtcgccatct ctaaggagaa
     4681 aaacggtcga gctctgacta atgccagccg taaggctgat ttgaagcccc tggctgatgc
     4741 ctacaacgaa gagagaaaga cccagtttgc taaactagga caattatcag accagatagc
     4801 gattctcgac tatcaagaac gttatcatgg agacacttgg aaactagcta aaaccttcca
     4861 atctttcatg agcgattttg tagaggctta tcgtcagaga aaacgacagg aaaatgcctt
     4921 tgaattcgct gatatcagcc attacaccat tgagatttta gagaatttcc cacaagttcg
     4981 tgagtcttat caggagcgct tccatgaagt catggtcgat gagtatcagg ataccaacca
     5041 tattcaagaa cggatgctgg aattgttgtc taatggccac aatcgcttta tggtgggaga
     5101 tatcaagcaa tccatctatc gtttccgtca ggcagacccg cagattttca atgagaaatt
     5161 ccaacgctat gcgcaaaatc cccaagaagg caggctcatt atcctcaagg aaaatttccg
     5221 tagtagttca gaagtgctgt cagcaaccaa tgatgtcttt gaacgtctca tggaccaaga
     5281 ggtcggcgaa atcaactatg ataacaagca ccagcttgtt tttgccaata ccaaactgac
     5341 tcccaatcca gacaacaagg cagcatttct cctctacgac aaggacgata caggtgagga
     5401 agaagagagt caaacagaaa cgaaactaac aggcgaaatg cgcttagtta tcaaggagat
     5461 tctgaaactt catcaagaaa aaggtgttgc ctttaaggaa attgcccttc tgacctccag
     5521 ccgcagtcgt aatgaccaga ttctcctcgc cctgtctgag tacggaattc ctgtcaaaac
     5581 tgacggagag caaaacaatt atctccaatc cctagaagtg caagtcatgc tagacactct
     5641 tcgtgtcatt cacaatcccc tgcaagacta cgccttggtt gcccttatga agtctccaat
     5701 gtttggtttt gatgaggatg agctagcacg tttgtccctt cagaaagcag aggataaagt
     5761 ccacgaaaat ctctatgaga aactggtcaa tgcacaaaaa atggcaagta gtcaaaaagg
     5821 cttgattcac acagctctag ctgaaaaact aaagcaattc atggatatcc tagcttcttg
     5881 gcgcttgtat gccaaacccc actctctcta tgacttgatt tggaagattt acaacgaccg
     5941 tttttattat gactatgttg gggctttgcc gaatggtcct gctaggcagg ccaatctcta
     6001 tgccctagca ctgcgtgctg atcaatttga aaagagcaat ttcaaaggtt tgtcgcgttt
     6061 tattcgtatg attgaccaag tcttagaagc ccagcacgat ttggcaagcg tggccgtcgc
     6121 accgccaaaa gatgcagtag agctcatgac catccacaag agtaaagggc tggagtttcc
     6181 ttacgtcttt atcctcaata tggatcaaga tttcaacaag caggactcta tgtcagaagt
     6241 cattctcagt cgtcagaatg gtcttggtgt caaatatatt gccaagatgg agacaggggc
     6301 agtagaagac cactatccta aaaccatcaa actctccatt cctagtctga cctataggca
     6361 gaacgaagag gaattacagc tagcaagcta ttctgagcag atgcgtttgc tgtatgttgc
     6421 tatgacgcgg gctgagaaaa agctctatct tgtcggcaag ggttctcgtg aaaagctgga
     6481 agccaagcaa tacccagcag ccaaaaatgg gaaactaaat agcaatacta gactgcaagc
     6541 acggaatttc caagattggc tttgggctat cagtaaagtg tttactaagg acaagctcaa
     6601 ctttagttat cgttttattg gcgaagatca gttgaccaga gaagctatcg gagagttgga
     6661 aaccaagagt cctctccaag atagctccca agcagacaat cgtcagtcag ataccatcaa
     6721 agaagctctg gaaatgctga aggaggtgga agtttataat actcttcacc gcgcagctat
     6781 tgaacttcct agtgttcaaa ccccaagtca aatcaagaaa ttctacgaac cagttatgga
     6841 tatggaaggt gtcgagattg ctggtcaagg tcagtcagta ggcaagaaaa tcagcttcga
     6901 tttgccagat ttttcaacca aagaaaaggt aactggagct gagattggta gtgctactca
     6961 cgaactcatg cagagaattg acctcagcca gcaactaacc cttgctagcc taacagaaac
     7021 actcaaacaa gttcaaacta gccaagctgt cagagacaag atcaatcttg ataaaattct
     7081 tgctttcttt gacacagtac tcggtcagga aattcttgct aataccgacc atctctatcg
     7141 cgagcaacct ttctccatgc tcaaacgaga ccaaaagagt caggaagact ttgttgtccg
     7201 tggtatcctt gatggctatc tgctttacga aaacaaaatt gttctgttcg actacaagac
     7261 agaccgctat gatgaaccaa gtcaactcgt agaccgctat cgtggtcagt tagctctata
     7321 cgaagaggct ttatcacgag cctattcgat taaaaatatt gaaaaatact tgattttact
     7381 cggtaaagac gaggttcaag ttgtaaaagt ataacttata ctctttgaaa atctcttcaa
     7441 accacgtcag cttcgccttg ctgtatatat ggttactgac ttcgtcagtt ctatccacaa
     7501 cctcaaaaca gtgttttgag ctgattgcgg ctagctttct agtttgctct ttgattttta
     7561 ttgagtatta gaaaggagac ctcatgacac ttccagttag aaaatccctg cacgatgcag
     7621 ttttacaggc ttcgaaagcg gatacttggg aacaagctac caaggaatgg aatgaagttt
     7681 ccttgatttt taacggtatt ggccgtagca attgtgtctg tggaaatgcc atcaaatacg
     7741 cttatgaact ctttaacggt gtcacaggcc aacgcctctt tccaattggt agcgactgtg
     7801 ttcgtcattt tcatcgattg tcccttgacc agcaattgga agaggaagaa aaactgctca
     7861 gaaaggttga aaatctaacc agaaaagctc agaaaaagga aaaaatcaag gtcaataaaa
     7921 gtgactttga cgagcgactt ctaaaatggc tctgggaaaa aggtgttttc aaacccaatc
     7981 gtggcaatca atttgcgcct gagagagact accagctttt cctagaagtc tttcagggag
     8041 gaagttggac caaggcggaa ccaaagaaaa aggctcggat ggaagaagtc cttgaaaagt
     8101 gtatcaaacc ctttttactt gggaaatcgg atgaccaact ctaccttgtc aagctaggca
     8161 aggagaaaat tgactacgaa caagaacttc gtatccaggc agagaaagaa cgcaagaaga
     8221 gggataaaat cgccaagcaa tatgcagaca atctggttct agccatggga cccgcagagc
     8281 gtgcttatca agattacttt ggctttacag agaccctgac tcaagaagaa cgcaagtggg
     8341 aaaaaattct ttttggtaaa aatagagcag aacgagctat caaggccaaa caataccaaa
     8401 aagagctgga aaaagaccaa cgaattgcca gtcaggatcc aattgaaaga aaacagaagc
     8461 agacttggct cctcaattcc tattttcgtg agcttcctga agaaaaagct cgattttcta
     8521 ggctcttatt agaatatcga aaaagtggtg aagtaccctt ttcaactgaa tacctgtcag
     8581 atcatctcat cgactttttc tataaaatga aagcctttga atttgaaatc gcaccagaac
     8641 aagtccgaga ttttctaaaa aaaagcctgc aggaagacca tcgctcatca gcacaaggaa
     8701 gctggataga aggaattctc ctcaactgcc tcaaaccatt tttagaacga ttagtgatat
     8761 gagtaacacc taccgtgtaa atttcatggt aggtgttatt gtacccccaa ataatataaa
     8821 acaaagtatg aaagcgcatc tcaattttaa gacttttttg taaattaaga gtataattaa
     8881 ggtatactgc cttttctata gataatagat aatacactaa tataataaag gtctttgtaa
     8941 catatataaa ttttatttca aggaggaata atggaaaagt attttggtga aaaacaagag
     9001 cgtttttcat ttagaaaatt atcagtagga cttgtatctg caacgatttc aagtttattt
     9061 tttatgtctg tattagctag ttcatctgtg gatgctcaag aaactgcggg agttcactat
     9121 aaatatgtgg cagattcaga gctatcatca gaagaaaaga agcagcttgt ctatgatatt
     9181 ccgacatacg tggagaatga tgatgaaact tattatcttg tttataagtt aaattctcaa
     9241 aatcaactgg cggaattacc aaatactgga agcaagaatg agaggcaagc cctagttgct
     9301 ggtgctagct tagctgctct gggaatttta atttttgctg tttccaagaa aaaggttaag
     9361 aataaaacgg tattacattt agtattggtt gcaggaatag gaaatggtgt cttagtttca
     9421 gtccatgctt tagaaaatca tcttttgcta aattacaata cggactatga attgacctct
     9481 ggagaaaaat tacctcttcc taaagagatt tcaggttaca cttatattgg atatatcaaa
     9541 gagggaaaaa cgacttctga ttttgaagta agtaatcaag aaaaatcagc agccactcct
     9601 acaaaacaac aaaaggtgga ttataatgtt acaccaaatt ttgtagacca tccatcaaca
     9661 gtacaagcta ttcaggaaca aacacctgtt tcttcaacta agccgacaga agttcaagta
     9721 gttgaaaaac ctttctctac taaattaatc aatccaagaa aagaagagaa acaatcttca
     9781 gattctcaag aacaattagc cgaacataag aatctagaaa cgaagaaaga ggagaagatt
     9841 tctccaaaag aaaagactgg ggtaaataca ttaaatccac aggatgaagt tttatcaggt
     9901 caattgaaca aacctgaact cttatatcgt gaggaaacta tagagacaaa aatagatttt
     9961 caagaagaaa ctcaagaaaa tcctgattta gctgaaggaa ctgtaagagt aaaacaagaa
    10021 ggtaaattag gtaagaaagt tgaaatcgtc agaatattct ctgtaaacaa ggaagaagtt
    10081 tcgcgagaaa ttgtttcaac ttcaacgact gcgcctagtc caagaatagt cgaaaaaggt
    10141 actaaaaaaa ctcaagttat aaaggaacaa cctgagactg gtgtagaaca taaggacgta
    10201 cagtctggag ctattgttga acccgcaatt cagcctgagt tgcccgaagc agtagtaagt
    10261 gataaaggcg taccagaagt tcaaccagcg ttatcggaag cagtcgtaac cgacaaaggt
    10321 gagcccgctg tccaaccaga gctatcggaa gcagtagtaa ccgacaaagg tgagcccgct
    10381 gtccaaccag agctatcgga agcagtcgta accgacaaag gtgagcccgc tgtccaacca
    10441 gagctaccgg aagcagtagt aagtgataaa ggtgaaccag cagtccaacc agagctaccg
    10501 gaagcagtcg taaccgacaa aggtgagact gaggttcaac cagagtcgcc agatactgtg
    10561 gtaagtgata aaggtgaacc taaacaggta gcaccgcttc cagaatatac aggaccacaa
    10621 gccagcgcta ttgttgaacc agagcaggta gcaccactac cagaatacac aggggtacaa
    10681 gccggatcga tagtcgagcc agagaaagta gaagcaccga aagaatatac tggtaaaatc
    10741 gaacaaccta gtgcagaaga tactaaacct gaaaatgagg caagctctac aaatggggaa
    10801 tcggaacgtc ctaaagataa aattaaagaa gaaaaacaag tcgacaagaa actagaactt
    10861 agaaatgttt caaacgtgga attatatacg gtagaaaata ataaatatcg acatattacg
    10921 gctgtagatg gtgctttaga tagttcattg aagtatttca tgaaggtaaa atcagaaaat
    10981 tttaaagata taatgttgcc tgttactaaa attgaaagta caacaaaaaa caataaagaa
    11041 gtatacaaaa ttgtggctca tgcagaaaat ctaattcaac acgaaaacaa tgttatttcg
    11101 aatgactata catattattt accaaaaact cagcaaagtg aaactggcgt ctatacatca
    11161 tttaaaaatt tagttgatgc aatgaatagt gaccctaatg gaacattcca tttaggtgca
    11221 actatggatg ctcgtgaagt tgaacttccg gatgatcaag aaagttatgt aaaaaatgaa
    11281 ttttacggaa aattaatagg tgaaaataat ggtaagtatt atgctattta taacttgaaa
    11341 aaacctttat ttaaaactct aaacaccgct actattcaga atctttcaat aaaagaagcg
    11401 aatgtttctt ctaaagaaga tgctgctaca atatcgaaag aagcaaaata caatactctt
    11461 atagataatg ttcattctga tggtattata gctggtgaac gtggaattgg tggattggtt
    11521 tcaaaagtag ataactcgag aatttctaat agtagtttta caggcagaat aactaacaca
    11581 tatgatacaa ctgcagggta tgaaattggt ggtttagtag gtaaactttc tggttcgctt
    11641 gcttctatag agaaatcaat agcatcgatt gacatagcct ctaatgcaaa atcaggagat
    11701 caaattgttg gtggtatagc cggagttgtt gagaaaagtg cgacaattaa gtatagctat
    11761 gttgaaggaa atgttaacaa tgtgcgacac tttggtaaag ttgggggagt tgcaggtaat
    11821 ctttgggata gagattcaca agatgtaagt aaatcaggta aactatcata tgttttaagt
    11881 gatgtcaatg ttacgaatgg taatgctata gctggctata atttcaatgg aataaaaacg
    11941 attgaaacat atagcaataa aaataataag gtagtaaacg tagtacaaga agatgatgag
    12001 gttgtgacta aagattcaga tgtacaaaga ggtacggtat tagatgctga taaagtaaaa
    12061 gagaaaaagg tagaattggt ttctaaacac agcactaaag tagaagattt tgattttact
    12121 tcaagataca atactaatta caatgaagta acaggttacc aacagtcgag agaacaagta
    12181 tataaaaata ttgaaaaatt acttcctttc tataacagag aaacaatagt taagtatggt
    12241 aatttagtag aggataacag tgacttattt actaaaaaac ttctatcagt tgttcctatg
    12301 aagaacaatg aagtaataac tgatattaat aaaaataaac aagaaataaa caaattgtta
    12361 ttacattttg aaggtaacaa atcacgagta ttaaatattg catataagaa tgatttctct
    12421 aaagttgctg agtatgatat agctaataca aaattaatgt atacacctaa tacaatgctg
    12481 catgattata ataatattgt aaaaactatt ttaaatgatc tgaaatctgt tcaatatagt
    12541 tcagctgacg ttagaaaagt attagatatt tctggtaata taaaattaac agagttatat
    12601 ttaggtgaac aatttgaaaa aacaaaagca aatatagaag acagtttatc aaaactactt
    12661 actgcagatg ctgcaatagt ggaaaataac aataaggtaa tagataatta tgtaattgag
    12721 aaaataaaaa ataataagga agccttattg ttaggattaa cgtatttaga acgctggtat
    12781 aattttaatt atggagaaac caatgctaaa gatttaataa tgtaccactt ggatttcttt
    12841 ggtaaatcaa atagttctgc attagataat gttattgagt taggtaaatc tggatttaac
    12901 aatttattag caaaaaataa tgtaattact tataatgtat tattagcgaa aaattatgga
    12961 actgaaagtt tatttaaagc attagaagga tatagaaaag tattcttacc aacaatttct
    13021 aacaatgagt ggtttaaaaa acaaacaaaa gcatatattg tagaagaaaa atctacaatt
    13081 gaagagggaa gagaaaaaca aggaaaagag ggaactaaat attctattgg ggtatatgat
    13141 agattaacta atccatcttg gaaatatcaa agtatggtct taccactatt aactttacct
    13201 gaagaaaaaa cagtgtttat gatagcgaat atttccacta ttggatttgg agcctatgac
    13261 agatatagaa gttcagaata tcctaaggga gaaaagttaa acaaatttgt agaagataac
    13321 gccaaagaag cggctaaacg atttagagat cattatgatt attggtataa gatattagat
    13381 aacgataata aagaaaaatt atatagaagt attctagtat atgatgcttt caaatttggt
    13441 actgacaaag ataaagacaa agtaactcat caagctactt ttgaaactga tcatccagct
    13501 ataaaatact tctttggacc tgcgggaaat aatgttgttc ataatggaca cggagcatat
    13561 gctaccggag atgcattcta ttatatggca tatcgtatgt tggataaaga tggagctgta
    13621 acatatactc atgaaatgac acataactca gatagagaga tttatcttgg aggatatgga
    13681 agaagaagcg gattaggacc tgagttttat gcaaaaggat tattacaagc tccggatcat
    13741 ccatatgatc caacaataac aattaactct gtcttgaaat atgaggattc agaaaattca
    13801 acaagacttc aagtagctga tccaactcaa cgatttaata gtgcagaaga cctacataat
    13861 tatatgcata atatgtttga tgtaatttat atgttagaat atttagaagg aaaagctgta
    13921 gctaatttag aaacaaatca aaaatatgag ttactaagaa aaattgaaaa taaatttgat
    13981 cttgatcaag acggtaataa tgtatacgcg acaaatgttg ttcgcagatt aacgatggat
    14041 gaagttaata aattaaattc ttttgatagt ttaattgaaa atgatattat tactagcaga
    14101 gggtacaaag accaagaata taagagaaat ggttattata caattgattt attttcaccg
    14161 atttattcag ctcttagtgg agagaaaggg actcctggtg atttaatggg acgccgtata
    14221 gcattcgaat tattagctgc aaaaggatat aaagagggca tggtacctta tatctcaaat
    14281 caatatgaaa aagatgcaaa agcggcggga agtaagatta attcttatgg taaagaagta
    14341 ggtttagtta cagatgaatt agtacttgaa aaagtattta atggtcaata taaaacttgg
    14401 acacaattca agaaggatat gtataaggaa agagaaaaac aattcagtaa actaaataga
    14461 gttaacttta ttaatcctaa taatccattg agtcgtcaac gaaatgtatc ggttacggat
    14521 ataggtgtac tagagagaat gattgtcgaa gcagtgagag atgatgcgca agatgacgta
    14581 gcaaaatttt acccagaaac aaatagtaga gttcttaaac tgaaaaaagc aatatataaa
    14641 gcttatcttg accaaacaaa tgattttaga agttcaattt ttgagaataa aaaatagtgt
    14701 ttactattag gaaataaaaa gtgaatgtgg aggattagaa ttgtcattat taaaaaaaga
    14761 taaattttcc attcggaaaa taaagggtat tgttggttcg gtattcttgg ggagtctttt
    14821 atttgcaccg tcagttgttg gtgcatcgac ttatcattac ttagattata gtagtttgac
    14881 acaaactgaa cgtgatcaac tcaaacaagg tagacctgat gaatccaaag agtcatatgc
    14941 tttggattat gaaaaagacg cactaccaaa cacaggaagt tctcaatcta ttatgactgc
    15001 attgggtttg ttagctatcg ggagtcttat tgtgattatc acaaaagata atagaaataa
    15061 aaaaatagct acatttttga ttgtaggggc gacaggttta gttacactat caactgcttc
    15121 agcacttaat ttgaatgcta acatccatga gtctggacgt gatggtgtgt tgcaaatttc
    15181 tggttacaga tatgttggtt acttggagct tgatgataaa actgtttcgt cggtttctcc
    15241 agcatcaact gtttcgccag ttgagcaacc caaagttgta acagagaaag gggaaccgga
    15301 ggttcaacca gccttacccg aagctgttgt gactgacaaa ggcgaaccgg aagtacaacc
    15361 gacattacca gaagctgttg tgactgacaa aggcgaacca gaagttcatg agaagcctga
    15421 ctatacgcaa ccaataggtg caaaccttgt tgagcctgaa gttcatgaga aactag
//
LOCUS       DAVDWM010000046        15204 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVDWM010000046 DAVDWM010000000
VERSION     DAVDWM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.046"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1..992
                     /locus_tag="VUW49_001710"
                     /pseudo
     CDS             1..992
                     /locus_tag="VUW49_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1066..1596)
                     /locus_tag="VUW49_001711"
     CDS             complement(1066..1596)
                     /locus_tag="VUW49_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HET7981598.1"
                     /translation="MLNEKRKNYVEDKRQNFPSLPNWKMDLFSEEEKRNYFQTTCEIS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDVEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSDIHKLGI"
     gene            complement(1590..2057)
                     /locus_tag="VUW49_001712"
                     /pseudo
     CDS             complement(1590..2057)
                     /locus_tag="VUW49_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2606..3283
                     /locus_tag="VUW49_001713"
     CDS             2606..3283
                     /locus_tag="VUW49_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET7981599.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQIRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            3270..4067
                     /locus_tag="VUW49_001714"
     CDS             3270..4067
                     /locus_tag="VUW49_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET7981600.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4084..5187
                     /locus_tag="VUW49_001715"
     CDS             4084..5187
                     /locus_tag="VUW49_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7981601.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            5194..5385
                     /locus_tag="VUW49_001716"
     CDS             5194..5385
                     /locus_tag="VUW49_001716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981602.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            5488..6507
                     /gene="galE"
                     /locus_tag="VUW49_001717"
     CDS             5488..6507
                     /gene="galE"
                     /locus_tag="VUW49_001717"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7981603.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKVMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            6509..7462
                     /locus_tag="VUW49_001718"
     CDS             6509..7462
                     /locus_tag="VUW49_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7981604.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(7524..7730)
                     /locus_tag="VUW49_001719"
     CDS             complement(7524..7730)
                     /locus_tag="VUW49_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7981605.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            7782..8258
                     /locus_tag="VUW49_001720"
     CDS             7782..8258
                     /locus_tag="VUW49_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET7981606.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            8267..8938
                     /locus_tag="VUW49_001721"
     CDS             8267..8938
                     /locus_tag="VUW49_001721"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7981607.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9069..9407
                     /locus_tag="VUW49_001722"
     CDS             9069..9407
                     /locus_tag="VUW49_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET7981608.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            9410..10039
                     /locus_tag="VUW49_001723"
     CDS             9410..10039
                     /locus_tag="VUW49_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET7981609.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            10279..11430
                     /locus_tag="VUW49_001724"
     CDS             10279..11430
                     /locus_tag="VUW49_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7981610.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWRAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASCLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            11748..12497
                     /gene="truA"
                     /locus_tag="VUW49_001725"
     CDS             11748..12497
                     /gene="truA"
                     /locus_tag="VUW49_001725"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7981611.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            12487..13260
                     /locus_tag="VUW49_001726"
     CDS             12487..13260
                     /locus_tag="VUW49_001726"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7981612.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            13238..13699
                     /locus_tag="VUW49_001727"
     CDS             13238..13699
                     /locus_tag="VUW49_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7981613.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(13914..15136)
                     /locus_tag="VUW49_001728"
     CDS             complement(join(13914..14797,14797..15136))
                     /locus_tag="VUW49_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET7981614.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMNKALLEGCSQRSVS
                     LDYALPNQGMLSFWLAQYKKNGYTIVEKTRGRPAKMGRKRKKTWKEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGIDKDKEIKTEIQAIYNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDPNSTQKLYLSPVLDGFNS
                     EIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQA
                     SMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVK
                     LKGLSPVQYRTKSFG"
BASE COUNT         4392 a         2857 c         3268 g         4687 t
ORIGIN      
        1 aaatgggacg taatccaaaa acgagacctg aagagaggac agaattagaa cgtcttcaat
       61 cagaaaatga gtacctgaga gcggagaatg ccatcctaaa aaagttaaga gaactccgat
      121 tgaaggagga aaaagagaaa gaagaaagac agaaattgtt caagaattaa tgactgagtt
      181 ttcgttagat cttcttctaa aagtcattaa actagctcgt tggacctact actatcactt
      241 gaaacagcta gataaaccag ataaggacca agagcttaaa gctgaaattc aatccatttt
      301 tatcaaacac aagggaaatt atgcttatcg ccgggttcat ttagaactaa gaaatcgtgg
      361 ttatctggta aatcataaaa gagttcaagg cttgatgaaa gtactcaatt tacaagctaa
      421 aatgcgacag aaacgaaaat aaaggagacg ttggtaagaa ggcagagaat ctcatttaag
      481 gccaatttga aggctctaaa acaatggaaa agtgctacac agatgtgaca gaatttgcca
      541 ttccagcaag tactcaaaag ctttacttat caccagtttt agatggcttt aacagcgaaa
      601 ttatcgccta taatctttca acttcaccca acttagaaca agtacaaaca atgttggaac
      661 aggcattcaa agagaagcac tacgagaata cgattctcca tagtgactaa ggctggcaat
      721 accgacacga ttcttatcat cggttcctag agagtaaggg aattcaagca tctatgtcac
      781 gcaagggaaa cagccaagac aacggtatga tggaatcttt ctttgggatt ctgaaatcgg
      841 aaatgtttta cggttatgag aagtcgtttc agtcgcttaa gcaattggaa caagccatta
      901 tagactatat tgattactac aacaataaga gaattaaggt aaaactaaaa ggacttagcc
      961 ctgtgcaata cagaactaaa tccttcggat aaattatttg tctaactttt ggggggcagt
     1021 acatttttgg tatatataaa atttgtagga gctatatcta caattttata ttcccagttt
     1081 atggatgtca cttactatat tcacaatgtt atccagtgtt ttttctctaa tatttaagga
     1141 gtgttctgtt tctcgaataa attcttcaaa gtttaacccg tcaacttgtt cctgaacaag
     1201 aaaataatca tccacgatat aaaattcatc agttaaatta gtagtataac ttttatcggc
     1261 taattttttt agcatgtgag cttcattttt tatatcatca agagctgtcc attctccttc
     1321 aacatcataa ttcacaaaag gtcttgactg cttgatgatt actttttgcc cgtccgattt
     1381 tctaattgcc cgataaacat ttcctttatt tgatctctta ataatttttt ccattttgta
     1441 tttatttatt gcagagtctt tacttgaaat ttcacatgtg gtttgaaaat aattcctttt
     1501 ttcttcttct gaaaataaat ccattttcca gttagggaga ctagggaaat tttgtctctt
     1561 atcttctaca tagttcttcc ttttttcatt tagcaataaa tagatgactt ttttattttt
     1621 ttcatcataa gcttgttttt ttaaaaaagc cccatatcta taatgaactg gagaatgcat
     1681 tccacattga tagtcagata gtatttttgg agccttaaat tctgacagtt tattcgtaag
     1741 attacaaatc atactcttgg cttcagattc tgacttagga tatagagtta taaatttgtt
     1801 agcagtaggg ttcatttccc taggggaatt aatttttttt aattcctcta aatttttaac
     1861 gactttaaag ctacaattat ttagtttgga tagtttatac acaatcttaa aatatctaca
     1921 gcatctttga tttgagaact tatgtgtatt ttccagcctt gtgcaggcat cttatctgac
     1981 aaatgatagt aattccaaat atcatcttgt tggaaatttt tatcacattt taaacattct
     2041 ttctctaata aatccataac aaactcctaa ttattaaaat aggctagaga cacagcacta
     2101 gcctattccg taagtaaagc tacttatttt atataaaata taactagcga cctgtaatta
     2161 aacaagtaaa agaacaatac ttagggagac ctgtgacaat gatatcttcc tctggaagta
     2221 gttctaattc aaacaatgtt tgttccatga ctgtatcctc ctgttatgat aaattccaga
     2281 aagcatagag caaattcgtg gttcgtctga ctaaatttct tatctgaaca ccaccttcaa
     2341 atacaaattt tagtcaaact tttatttttt ctaactttct aattatattc tatcatgtgt
     2401 ttttatatgt caagaatttt ctgaataaac tgtttatttt ttattttttg acatttttgc
     2461 aaattgttta aaatctttca attggattca cttattactt taggacattg aaacttcttt
     2521 cgccagaggt gcttatatta ctgggaagtt gaagattgaa atgtggtata ataagaggta
     2581 atagagtttt ggaaagtgag agaagatgat ttcaaagaga ttagaattgg tggcttcctt
     2641 tgtgtcacag ggggctattt tactagatgt gggaagtgac catgcttatc tgcctatcga
     2701 gttggttgag agaggccaaa tcaaaagcgc tattgcaggt gaggtggtgg aaggtcccta
     2761 tcagtctgcg gttaaaaatg ttgaggctca cggcctaaag gagaaaatcc aaatccgttt
     2821 agccaatggc ttggcagctt ttgaagagac tgaccaagtg tctgtcatta ccattgctgg
     2881 catgggtggt cgtttgattg ctaggatttt agaagaaggt ttggggaagt tagctaatgt
     2941 agagcgtttg atcctccagc ccaataatcg tgaagacgac ttgcgtatct ggctacagga
     3001 tcatggattc cagattgtag cagaaagcat cttagaagaa gctggaaagt tttatgagat
     3061 tttggtggtg gaagcaggac aaatgaagct atcagccagt gatgttcgct ttggtccctt
     3121 cttgtccaaa gaagtcagtc cagtatttgt ccaaaaatgg caaaaagaag ctgagaagct
     3181 agagttcgcc ctcggacaaa tcccagaaaa aaatctggaa gaacgtcaag ttctagtaga
     3241 taagattcaa gctatcaagg aggtgctcca tgttagcaag tgaagtgatc caagcttatg
     3301 aagccttttg ccctcaggaa ttttctatgg agggagacag ccgtggtctg caaattggca
     3361 ctttagacaa gggtatccaa agggtcatgg ttgccctcga tattcgtgaa gagacggtgg
     3421 cagaggccat tgaaaagggt gtggacttga ttatcgtcaa gcacgcgcct atctttcgtc
     3481 ctatcaagga cttgcttgcc agccgtccgc aaaatcagat ttacatcgac ctgattaagc
     3541 atgatatcgc agtttatgtc agccatacca atattgatat cgttgaaaat gggctcaatg
     3601 actggttttg tcagatgcta ggaatcgagg agacgactta tctgcaggaa acaggtccag
     3661 aacgtggaat tggacgtatt gggaatattc agcctcagac attttgggaa ttggcccaac
     3721 aggtcaagca agtctttgac ctagatagcc ttcgaatggt gcattatcaa gagaatgatt
     3781 tgcagaagcc tatttcaaga gtagcaattt gtggtggaag cgggcagtct ttctataagg
     3841 atgctttggc aaagggggca gatgtctata tcactggcga catctactac catactgctc
     3901 aggatatgct gtctgatggc ttgttagcat tggatccagg tcactatatc gaagtgcttt
     3961 ttgtggaaaa aatcgcagca ctccttactc aatggaaaga gaaaaaaggc tgggaactag
     4021 agattttacc tagtcaagca tcgaccaatc ctttccgtca tatctagtta gaaagtgaag
     4081 acaatgaaaa aagttgccat tattggagca ggaattgtgg gagcaacagc tgcctactac
     4141 ctctcgagag aaagtgacct agaggtgacc gtttttgacc atgagcaagg tcaagccacc
     4201 aaggccgcag caggaattat cagtccttgg ttttccaaac gccgtaataa agcctggtac
     4261 aagatggcgc gcttgggggc tgatttttat gtggatttat tagctgattt agagaaatca
     4321 ggacaagaaa tcgactttta ccagcgttcg ggagtctttc tcttgaaaaa ggatgaatcc
     4381 aagttggaag aactttatca actggccctc cagcgcagag aagaatctcc cttgataggg
     4441 caattagcca ttctggacca agcttcagct aatgaattat tccctggttt gcagggattt
     4501 gaccgcctgc tctatgcttc tggtggagcg agagtagatg gccaactctt ggtaactcgt
     4561 ctgctagaag ccagtcatgt caagctggtc aaagaaaaag tgactctgac actcttatca
     4621 tcaggctacc agattggcga agaggtgttt gatcaggtta ttttggcaac gggagcttgg
     4681 ttgggggaca tgttagagcc tttaggttat gaagtggatg tccgtcctca aaaaggacaa
     4741 ctacgagatt atcagcttgc ccaagacatg gaagattacc ctgttgtcat gccagaaggg
     4801 gagtgggatt tgattccctt tgcaggtggg aaattatcct tgggcgctac ccatgaaaat
     4861 gacatgggat ttgatttgac ggtagatgaa cccttgctcc aacaaatgga ggaggcggcc
     4921 ttgcctcatt acccagtttt agctagagcg acttcaaggg ctgagcgtgt gggaatccgt
     4981 gcctatacca gtgatttctc tcccttcttt gggcaggtgc cagaattggc aggtgtctat
     5041 gtagccagtg gactaggttc atcaggcctc acaactggtc ctatcattgg ttaccatcta
     5101 gcccaactga tccaagacaa ggagttgacc ttggaccctg aaaattaccc aattgaaaac
     5161 tatgtcaaac gagtaaaaag cgaataatac tcaatgaaaa tcaaagaaca aactaggaaa
     5221 ctagccgcgg gttactcaaa gcactgcttt gaggttgtgg atagaactga cgaagtcagc
     5281 tcaaaacact gttttgaggt tgtagataga actgacgaag tcagtaacca tacccatggc
     5341 aaggcgacgt tgacgtggtt tgaattggat tttcgaagag tataagcatt ttactgaaat
     5401 tttatcaggt agtttaggat ggcaaatgac attccctgtc aaaaatggta aaataagaaa
     5461 aaataatctg agaatcgagg aaaaaagatg caagaaaaga ttttggtaac tggtggtgcc
     5521 ggttttattg gaacccacac tgttattgaa ttgatccaag caggccatca agttgttgtg
     5581 gtggataatc ttgtcaatag caatcgtaag agtttagaag ttgttgaaag aatcacagga
     5641 gttgagattc ctttctatga ggcagatatt cgtgacacag ataccctcag agatattttc
     5701 aagcaggaag aaccgactgg tgtcattcac tttgctggtt tgaaggctgt tggcgaatca
     5761 acacgtatcc ctcttgccta ctatgacaac aatatcgctg gaactgtcag ccttttgaaa
     5821 gtcatggaag aaaacaactg taaaaacatc atcttcagtt cttctgcgac agtttacggg
     5881 gatccgcaca cagtgcccat cttggaagat ttcccacttt cagtgaccaa cccatacggt
     5941 cgtactaagc tcatgctaga ggaaattttg actgatattt acaaagcaga ctcagaatgg
     6001 aatgttgtct tgcttcgtta ctttaaccca atcggagccc atgagagtgg tgatttggga
     6061 gaaaatccaa acggtattcc aaacaatctc ttgccatatg tgactcaagt agccgttgga
     6121 aaattagagc aagtgcaagt gtttggagac gattacgata cggaagatgg aacaggtgtt
     6181 cgtgactata tccacgttgt cgatttggct aagggtcacg ttgcagcttt gaaaaaaatc
     6241 caaaaaggtt caggactaaa cgtttataac cttggaactg gtaaaggtta ctcagttctt
     6301 gaaattatcc aaaacatgga aaaagcggtg ggacgtccta ttccttaccg catcgtagaa
     6361 cgtcgcccag gtgatatcgc tgcctgctac tcagacccag caaaagctaa agcagaactc
     6421 ggttgggaag cagaactcga catcacccaa atgtgtgaag acgcatggcg ttggcaaagc
     6481 aagcatccaa atggatttga agactaagat gatgatttca atcatcgtcc cttgtttaaa
     6541 cgaagaggaa gtacttcctc ttttttatca ggctctggaa gctttacttc cagatttgga
     6601 aacagaaatc gagtatgtct ttgtcgatga tggatcaagt gatgggacct tggaactctt
     6661 aaaggcctat cgggagcaaa atccggcagt tcattatatt tctttctctc gaaattttgg
     6721 caaagaagca gccctttatg caggcttgca atatgcgaca ggagatttgg tggtggtgat
     6781 ggatgcagac ctccaagatc ctcctagtat gttgtttgag atgaaaaatg tactagacaa
     6841 aaatgtagac ttggactgcg ttgggacacg gagaactagt cgggagggag aacccttctt
     6901 tcgcagtttc tgtgctgttc tcttttatcg cctcatgcaa aaaatcagcc cagtagctct
     6961 gccgtcgggt gtccgtgact ttcgtatgat gagaaggtct gtggtcgatg ccattttaag
     7021 cttgactgag tccaatcgtt tttctaaggg actctttgcc tgggtcggct ttaaaaccca
     7081 ctatctggac tatccaaatg tcgaaaggca ggctggcaag accagttgga gttttaggca
     7141 actctttttt tactccattg aagggattgt taatttttca gatttccctt tgactatagc
     7201 ctttgtagct ggtctcctat cttgttttct ttctctgctg atgacctttt ttgttgtggt
     7261 tcggaccctc attttgggca atccgacatc tggttggacc tctctgatgg ctgttattct
     7321 ctttcttgga ggcattcaac tcttgaccat tgggattctc ggcaagtata tcagtaagat
     7381 ttatttagag actaaaaaaa gaccacttta tcttatcaaa gaaaaaagtg accttcctga
     7441 ttttacagaa aaaataaagt gaaaagacta taattttaca tggaaatgtg ctaaactaga
     7501 gggagtggat tatcgccatt gatttaggct tcctggtttc caatcagggc gcgagttggg
     7561 caatttttaa cagcctctaa gacatcctga ctaggagaaa tttctttttc cagttggtca
     7621 gggtcatcgt aaaaacgcac gattccatta tcgtggtaat caaataaatc agaataagtt
     7681 tggcaaagcc cacaggcgat acatcgttca ggtataagtg tgattttcat gttcatattt
     7741 atattgtaat aagaaaagta aaaaaaaaca aggagtaagg tatggcaaaa gaaccgtggc
     7801 aagaagatat ttatgatcaa gaagaatcaa gagcagagcg tcggcatcga aaccacggag
     7861 gggctgatag gatggctaat cgtattttga cgatcctagc tagtattttc tttgtaattg
     7921 tggtggtgat ggtcatcgtt ctcatctatc tatcatcggg ggggagtaat cgcacagcag
     7981 ccttaaaaga ctttcatgat tctgatgcaa gtgtagtaca aatctcatct tcaagcagtt
     8041 ctcagcctga gcagagttca gagccagaat ctacttctag tagttcagaa gaagctgcta
     8101 atcctgaagg aacgattaaa gttctcgcag gagaagggga agcagctatt gccgctcgtg
     8161 caggaatctc cattgctcag ttagaggcct tgaatcctgg gcacatggct acaggatctt
     8221 ggtttgctaa tccaggtgat gttataaaaa taaaatagga gccgatatga aaacaattca
     8281 aattgctatt gatggtcctg cttccagcgg taagagtacg gtcgcaaaga ttattgctaa
     8341 ggattttgga ttcacctacc ttgatacagg agctatgtat cgtgcagcga cctatatggc
     8401 tcttaagaac caattaggag ttgaagaagt cgaagccctt ctagccttgt tggaccagca
     8461 tccaatcagc tttggacgtt cagaaactgg agaccagctt gtttttgtag gagatgtgga
     8521 tattacccat cctatccgtg aaaatgaagt gaccaatcat gtttctgcta ttgcagcaat
     8581 tcctgaagtg cgtgagaaac tggtttctct ccaacaagaa attgcccagc aaggcgggat
     8641 tgtcatggat ggtcgcgata ttggaactgt tgtattgccg caagcagaat tgaaaatttt
     8701 cctagtagct tctgttgatg agagagcaga gcgtcgttac aaggaaaata ttgccaaggg
     8761 aattgaaaca gaccttgaaa ccctaaaaaa ggaaattgct gcgcgtgact acaaggatag
     8821 tcatcgtgag acttctcctc tcaaacaagc agaggatgct gtctaccttg atacaactgg
     8881 tttgaacatt caagaagtag ttgaaaaaat caaagcagaa gctgaaaaaa gaatgtaaat
     8941 gtagaaaagc cgatagattg atatcggttt ttttctaaat ttgtcaaaat gctgatttta
     9001 gggtataata gttcctgttc gagaaatttt gattttcaaa gaatagtgaa tgatgataag
     9061 gagaaaccat gaacaattta ccaaattgtc caaaatgtaa ctcagagtat gtctacgaag
     9121 acggtgccct actggtttgc ccagagtgtg ctcatgagtg gaatcctgct gaagttgcag
     9181 aagtagaaga aggtcttgtc gctatcgatg ccaacggaaa taaattggct gatggtgata
     9241 cagtaactct catcaaggac ttgaaagtaa aaggtgcgcc aaaagatttg aaacaaggga
     9301 cgcgcgtgaa aaatatccgc atcgtagaag gcgaccacaa tatcgactgt aagattgatg
     9361 gttttggtgc catgaaactt aaatcagagt ttgtgaggaa gatttaagca tgtcaaaaca
     9421 ctataaactt gtattttata gccgtatctt cttgtttcta gcggctttta cgggagttta
     9481 tcttgaaatt actaggcatg gtggttttgg gatgcttctc tattacacgg ttctgtccaa
     9541 cctcttggta actattttta ccctttatct tctaaaggtt atgagccgtg taggtgaaaa
     9601 ttggcaaagg ccaagtctct tgcgcttaaa aggtggggtc accatgagta tcatgattac
     9661 ctgtgtgatt taccatttcc tcttggcgcc cattgcgact aatttctata ccctagaaaa
     9721 tttcctttgc cactatatcg ttcccatctg gtttttagcg gataccctct tttttgacaa
     9781 acagggtcaa tacaagattt gggatccagc agtgtggacc attttaccct ttctgtatat
     9841 gatgtttgct ctttttaatg gcttggttct aaaactcaat attccaaatg ccaaggataa
     9901 tccttttcct tacttctttt tgaatgtgaa caagggttgg aatgttgtgt ttaagtggtg
     9961 tctgattatc tttgttgcct atatggtagc aggatttatt ttctacttta tcaagcaaat
    10021 caagagaaag tcatcctaat actcttcgaa aatctcttca aaccacgtca gtttccatct
    10081 gcaacctcaa aacactgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt
    10141 ttcattgagt ataagataca agggattcat tttcgaattt aagaaggagt ctacaaacca
    10201 atgaggcttc ttcttttctt gcttatttga taactatcaa aaaagtagaa gattaagaga
    10261 aatatagaaa gagatttcat gaaacaattt ttagaacggg ccagcatttt ggctctctcc
    10321 ctcgttttga ttacctcctt ttccatttcg agtgccctac cagccatgtt tgactattat
    10381 cagggttatt ctaaggaaca aattgagctc ttggtgagct tgccttcctt tggaatcatg
    10441 atgatgttac tgctaaatgg tttcttagaa aaaatatttc ctgagcgctt acagattagt
    10501 ttgggcttgc tgattttatc attgagcggt acagctccct tctggtacca agcctatccc
    10561 tttgtctttg gaacacggct tctctttggt ttgggtcttg ggatgatcaa tgccaaggcc
    10621 atttctatta tcagtgaacg ctaccaagga aaaaggcgaa ttcagatgtt agggctacgc
    10681 gcttctgcag aggtcgttgg agcttctctc attaccttgg ccgtcggtca gttgttggcc
    10741 tttggttgga gagctatctt tctagcctat agtgctggat ttttggtgct gcccctttat
    10801 ctgctctttg tcccttatgg aaaatcaaag aaagaagtca agaaaagagc gaaggaagca
    10861 agttgtttaa ctcgagaaat gaaaggcttg atttttacct tagctatcga agcggcagtt
    10921 gtagtttgta ccaatacagc tattaccatc cgtattccaa gtttgatggt ggaaagagga
    10981 ttgggggatg cccagttatc tagttttgtt cttagtatca tgcagttgat cgggattgtg
    11041 gctggggtga gtttttcttt cttgatttct atctttaaag agaaactgct cctctggtct
    11101 ggtattacct ttggcttggg gcaaatcgtg attgccttgt cttcatcctt gtgggtggta
    11161 gtagcaggaa gtgttctggc tggatttgcc tatagtgtag tcttgacgac ggtctttcaa
    11221 cttgtctctg aacgaattcc agctaaactc ctcaatcaag caacttcatt tgctgtatta
    11281 ggctgtagtt tcggagcctt tacgacccca ttcgttctag gtgcaattgg cttactaact
    11341 cacaatggga tgttggtctt tagtatctta ggaggttggt tgattgtaat ctctatcttt
    11401 gtcatgtacc tacttcagaa gagagcttag gattgattcc taagtttttc ttttgatact
    11461 caatgaaaat caaagagcaa actatagttg attgagtttg gaatagtacg ctgtagctgc
    11521 taaaatattt ctagaaatta atttgacttt cctaatagag ttgttcatat cttatttcaa
    11581 tctactatag aaagctagcc gcaggttgct caaaatagtg ttttgaggtt gcagatggaa
    11641 actgacgtgg tttgaagaga ttttcgaaga gtatgagatt tttataccca tacaatattt
    11701 gttttaggtc cttgtttaca gactaatttc ttgataaaat agaggttatg acgagatata
    11761 aagcaactat ttcctatgat ggttatgcct ttgctggctt tcagcgccag tctcatgcgc
    11821 gtagcgttca ggaagaaatt gaaaaaacct tgaccagatt aaataaaggg caaaccatta
    11881 ctgttcacgg tgctggtagg acagatagtg gggttcatgc cctgggacag gtcattcatt
    11941 ttgacctgcc ttatcagatg gatgaggaga aactccgttt tgccttggat acccagtctc
    12001 ctgaagatat tgatgtgatt tcgattgagc ttgtggcaga tgattttcat tgccgttatg
    12061 ccaaacatag caagacctat gagtttattg tggatagagg acgtcccaaa aatcctatgc
    12121 gccgtcacta tgccacccac tttccctacc cactcgatgt ggaacgaatg cagattgcaa
    12181 tcaaaaagct agagggaacc catgatttta ccggttttac agcctctggg actagtgtag
    12241 aggataaggt tcgcaccatc acagaagcta gtttaatagt cgatgagaca ggacaatttt
    12301 tgacctttac cttttcagga aatggtttct tgtataaaca gattcgcaat atggtgggga
    12361 ctcttctcaa aatcggtaac aaccgcatgc cagtagagca gattgatctc atcttggaga
    12421 agaaggacag gcaacttgca ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga
    12481 ttcgttatga agaataatcg tattttagca ctttctggaa atgatatttt tagtggtggt
    12541 ggactgtcag ctgatttggc tacctatacc ttgaacggct tgcatgggtt tgtagcagtg
    12601 acttgtttga cagccttgac agaaaaagga tttgaagtct ttccaactga tgataccatt
    12661 tttcaacatg aattagatag cttacgtgat gtggaatttg ggggaattaa gattggtctt
    12721 ctccctactg tcagtgtggc tgagaaggcc ttggacttta tcaaacaacg cccaggagta
    12781 cctgtggtgt tggatcctgt cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag
    12841 ctctgccaag agttgattcg ctttttccct tatgtcagtg tgattacgcc taatctccca
    12901 gaagcagaat tattatccgg tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg
    12961 cagaaattgc atgatttagg agcgccagca gtcattatca agggaggcaa tcgtcttagt
    13021 caggacaagg ctgtggatgt cttttatgat ggacagacct ttactatcct agaaaatcca
    13081 gttatccaag gccaaaatgc tggtgcaggt tgtacctttg cctctagcat tgccagtcac
    13141 ttgattaaag gtgataaact tttgccagca gtagaaagct ctaaggcttt cgtttatcgt
    13201 gctattgcac aagcagatca gtatggagta agacaatatg aagcaaacaa aaacaactaa
    13261 aatcgccctt gtatccctat taaccgccct ttctgtggtt ctaggttatt tcttaaaaat
    13321 cccaacacct acaggaattc taactctttt agatgctggt gtcttctttg cggcctttta
    13381 ctttggtagt cgtgaaggag cggtagtcgg aggactagca agtttcttga ttgacctctt
    13441 atcaggctac cctcagtgga tgttctttag cttggtcaac catggcttgc agggattttt
    13501 cgcaggattt aaaggaaaaa gtcagtggtt aggccttatt ttagcaacta ttgccatggt
    13561 aggaggctac gccttgggtt ctactttgat gaatggctgg gcagcagccc tcccagaaat
    13621 tctaccaaat ttcatgcaaa atatggtagg gatgattgta ggatttattc ttagtcaaag
    13681 tatcaagaag attaagtaat actcttcgaa aatctcttca aaccacgtca gcttccatct
    13741 gcaacctcaa aacactgttt tgagcagcct gaggctagct ttctagtctg attttcattg
    13801 aatataaaga ggttgagtaa aaagtctttc aaataagaaa aatgaactgc ccccaaaagt
    13861 tagacagaaa aaatctaact tttgtactgc ccccaaaaag ttagacaaat aatttatcca
    13921 aaggatttag ttctgtactg cacaggacta agtcctttta gttttacctt aattcgtttg
    13981 ttgttgtagt aatcaatgta gtccacaata gcttgttcaa ggttttctaa agatctaaag
    14041 ttcttctcat aaccataaaa catctccgat ttcaaaatgc caaagaaaga ttccatcatg
    14101 ccgttgtctg ggctgttgcc cttgcgtgac atggatgctt gaattccctt actctctagg
    14161 aaccgatgat aagaatcgtg ttggtattgc cagccttggt cactatggag aatcgtattc
    14221 tcatagtatt tctctgtaaa ggcctgttct aacatactct tcacttgttc taagttgggc
    14281 gaagtagaaa gatgataagc aatgatttca ctgttaaaac catctaaaac tggtgataaa
    14341 tagagtttct gtgtgctatt tggatctgta tagcactttt ccattggcct cgatgcttcg
    14401 aactggcgtt gaatgagatt ctctgctttc ttgccaatct ctccttggta ggaagaatac
    14461 ttccgtttac ggcgaattcg agccgttaaa ccaagaattc tcatcaaccg ttgaactttc
    14521 ttatgattga ccacaaaacc acgattcctt agttctaaat ggattcgacg atagccatag
    14581 ttgcctttat gttcattata aatggcctga atttcggttt taatctcttt atctttatca
    14641 atcccatcca gttgtttcaa ctgataatag taagtcgagc gagctaaacg tgctgtttca
    14701 agaagaaaat ctagtcgaaa tcctcctgaa accatttctc taactgtctc tgcctttctc
    14761 gctctagggc ttcgtccctt tcctctaact cttttaactt ttttaggtag gccacctcag
    14821 tccgtaagcg ttcattctcc tcctggagtc gttctagttc tgtcatttct ttccaagtct
    14881 tcttccgttt acgtcccatt ttagctggtc tccctcttgt tttctcaaca atagtatacc
    14941 cgtttttctt gtattgtgcc agccaaaatg aaagcattcc ttggtttggg agggcataat
    15001 caagactaac acttctttgt gaacaacctt ccagtaaggc tttattcatc atttcttgtt
    15061 tcaatttaga agaatagtga cgattcttcc cctttttaac gatttctatg ccataacggt
    15121 caattaattt caccatgtac tttagaccag aaacatccac accaaatctt tttgaaagct
    15181 gtttgaagct ttgtccttgc tttc
//
LOCUS       DAVDWM010000047        14568 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVDWM010000047 DAVDWM010000000
VERSION     DAVDWM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.047"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            10..1122
                     /locus_tag="VUW49_001729"
     CDS             10..1122
                     /locus_tag="VUW49_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7981615.1"
                     /translation="MRTPFTVLPIILGNISQGLEVEVSSLGVLTSLPLLMFTLFSPFS
                     TRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLMVGASVAVINVLLPSLIQA
                     NQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGLILLLTLLCLATFLVWLPN
                     HRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFYTVMTWLPTMSIHAGLSSH
                     EAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGVVGISMLFFPINNFI
                     YWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGY
                     SFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            1261..1587
                     /locus_tag="VUW49_001730"
     CDS             1261..1587
                     /locus_tag="VUW49_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7981616.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            1574..2167
                     /locus_tag="VUW49_001731"
     CDS             1574..2167
                     /locus_tag="VUW49_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET7981617.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGIEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLAILAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            2160..3071
                     /locus_tag="VUW49_001732"
     CDS             2160..3071
                     /locus_tag="VUW49_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET7981618.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            3134..4198
                     /locus_tag="VUW49_001733"
     CDS             3134..4198
                     /locus_tag="VUW49_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981619.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEERITDLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(4270..5130)
                     /locus_tag="VUW49_001734"
     CDS             complement(4270..5130)
                     /locus_tag="VUW49_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET7981620.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVYGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(5405..6391)
                     /locus_tag="VUW49_001735"
     CDS             complement(5405..6391)
                     /locus_tag="VUW49_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET7981621.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(6663..8138)
                     /locus_tag="VUW49_001736"
     CDS             complement(6663..8138)
                     /locus_tag="VUW49_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET7981622.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(8242..9165)
                     /locus_tag="VUW49_001737"
     CDS             complement(8242..9165)
                     /locus_tag="VUW49_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7981623.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(9179..10108)
                     /locus_tag="VUW49_001738"
     CDS             complement(9179..10108)
                     /locus_tag="VUW49_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7981624.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(10427..10648)
                     /locus_tag="VUW49_001739"
     CDS             complement(10427..10648)
                     /locus_tag="VUW49_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981625.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(10602..10862)
                     /locus_tag="VUW49_001740"
     CDS             complement(10602..10862)
                     /locus_tag="VUW49_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981626.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            complement(11258..11869)
                     /gene="rpsD"
                     /locus_tag="VUW49_001741"
     CDS             complement(11258..11869)
                     /gene="rpsD"
                     /locus_tag="VUW49_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7981627.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(12064..13116)
                     /locus_tag="VUW49_001742"
     CDS             complement(12064..13116)
                     /locus_tag="VUW49_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET7981628.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(13113..13811)
                     /locus_tag="VUW49_001743"
     CDS             complement(13113..13811)
                     /locus_tag="VUW49_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981629.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(13978..>14568)
                     /locus_tag="VUW49_001744"
     CDS             complement(13978..>14568)
                     /locus_tag="VUW49_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HET7981630.1"
                     /translation="KAPWSDNGDAKNPALSPLGENVKTKGQYFYQLALDGNVAGKEKQ
                     ALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSP
                     SNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAML
                     PNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT         4188 a         3180 c         2759 g         4441 t
ORIGIN      
        1 ggtgtttcct tgcgaactcc ttttactgtt ttacccatta ttttgggaaa tatttcgcaa
       61 ggtctggagg tagaagttag ttcgcttggt gtcttgacca gcctgcctct ccttatgttt
      121 accctctttt caccattttc tacccgactg gctcagaaaa tcggcttgga gcatctcttc
      181 acctacagcc tcttcttctt gaccatcggc tcacttattc gactaatcaa tctgcccctg
      241 ctctatctag gaaccttgat ggttggggca agtgtcgcag tcatcaatgt cctgcttcct
      301 agtcttatcc aagccaatca accaaagaaa attggttttc tgaccacctt atatgtaacg
      361 tctatgggga ttgcaacggc tctggcttcc tatctagctg tgcccattac acaagccagt
      421 tcttggaaag gacttatcct cctcctcacg ttactctgtc tagcaacttt tttggtctgg
      481 ctcccaaatc accgctataa tcatagacta gctccacaaa ccaaacaaaa aagtcaaata
      541 aaggtcatgc gtaataaaca ggtttgggca attattatct tttcaggttt tcaatccttg
      601 atcttttaca ccgtcatgac ctggttacct accatgtcta tccatgcagg tctatccagt
      661 cacgaagctg gcttgctgac ttctatctta tctctgatta gcattccttt ttcaatgacc
      721 atcccaagcc tgacaaccag tttatctact cgcaaccgtc agctcatgct cactctggtt
      781 tcactagctg gtgtggtcgg catttccatg ctctttttcc caatcaataa tttcatttac
      841 tggcttgcca tccatctcct catcggaacc gcaaccagcg ccctcttccc ttatctcatg
      901 gtcaactttt cactcaagac aagcgcccct gaaaagacag cccaattgtc cggcctatct
      961 caaacaggag gctatatcct agcagccttt gggccaaccc tctttggtta cagttttgac
     1021 ctgttccact cttgggtacc atctgtagct gccctcttgc tcatcgatat cctgatgact
     1081 gtggccctct ttacagtgga cagagcggat aagatccttt aaacttctag ttttagctag
     1141 aagtttttta tatataaaaa ttttacacat ttctcttgac agggctttct tttagatgta
     1201 caatgtatgt gtagaaaaat tatatataaa aatcttacac attagaaaag gaggtttccc
     1261 atgtactttc caacatcctc tgccttgatt gaatttctca tcttggccgt actggagcag
     1321 ggtgattctt atggttatga gattagccaa accattaagc tcatcgctaa tatcaaagaa
     1381 tccacactct atcccattct caaaaaattg gaaggcaata gctttctgac aacctattct
     1441 agagagttcc aaggtcgcat gcgcaaatac tactccttga caaacggtgg tatagagcag
     1501 ctcttgaccc taaaagatga atggacactc tatacagaca ccatcaatgg catcatagaa
     1561 gggagtatcc gccatgacaa gaactgaata cctgactcag ctagaactct atctcaagaa
     1621 actacctgaa gctgaccgta tcgaagccat ggactatttc agagagctct ttgacgatgc
     1681 tggaatcgaa ggagaagaag aactcatcgc tagtttggga actcccaaag aagcggccca
     1741 cgaagttcta tccaatcttc tcgataaaaa aatcaatgaa gcacccgctc aaaaaaataa
     1801 ccgacaaatt ttacatatcg ccttgttagc catccttgca gcacctatcg gcattcctct
     1861 gggaatcgcc atcctcgtga ccctgttcgc aatccttgta gccgctttga ctgtcattct
     1921 ggctttcttt gcagtttcca tactgggtat catcggcgga ttcctatttt tagttgaaag
     1981 tttcactgtc ctcgcccaag ccaaatcagc ctttatcttg atttttggtt ctggtttact
     2041 ggctatcggt gcttcttcgc tagttttact tggcatttcc tatgtaactc gcttcttcgg
     2101 tctactcatt gttcgtctgg tacaatttgt tcttaaaaaa ggaaagagag gtaatcagca
     2161 tgcgtaaatg gacaaaagga tttctcatct ttggtgtggt gactaccgtt atcggcttta
     2221 tcctgctttt tgtaggtatc caatctgacg ggattaagag cctactttcc atgtccaaag
     2281 aacctgtcta tgatagccgt acggaaaagc taacctttgg caaggaagtc gaaaacctag
     2341 aaattactct ccaccaacac gcgctcacca tcacagactc tttcgatgat caaatccaca
     2401 tttcttacca tccatctctt tctgctcacc atgatcttat caccaatcag aacgatagaa
     2461 ctctgagtct cactgataag aaactgtctg aaactccgtt tctctcttct ggaattggtg
     2521 ggattcttca tatcgcaagt agctactcta gtcgttttga agaagttatt ctccagctac
     2581 caaaagggag aactctaaaa gggatcaaca tctcagccaa tcgcggacaa accaccatca
     2641 taaatgctag ccttgaaaat gcgaccctca atacaaacag ctatatcctc cgaattgaag
     2701 gaagtcgtat caaaaacagt aaactcacaa cgcccaatat cgttaatatc tttgatacag
     2761 ttcttacaga tagtcagcta gagtcaacag agaatcactt ccacgctgaa aatatccaag
     2821 tccatggcaa ggttgaactg actgccaaag attatctcag aatcatccta gaccagaaag
     2881 aaagtcaacg aattaactgg gacatctcaa gcaactatgg ttctatcttc caattcacaa
     2941 gagaaaagcc tgaatcaaga ggtacggaat taagcaaccc ttacaaaact gaaaaaaccg
     3001 atgtcaagga tcaactcatt gcgagatctg atgataatat tgatctaata tccacaccaa
     3061 gcagacgttg acaaaaacgc ttacatctgc tactatggaa gcatatttat ctaactaaaa
     3121 aggaggttct accatgaaac aagaatggtt tgaaagtaat gattttgtaa aaacaacaag
     3181 caagaacaag cctgaagagc aagctcaaga ggttgcagac aaggctgaag aaaggataac
     3241 cgatctcgat acaccaattg aaaaaaatac tcagttagag gaggaagtct ctcaagctga
     3301 agtcgaattg gaaagccagc aagaagagaa aattgaagct cctgaagaca gtgaagcgag
     3361 aacagaaata gaagaaaaga aggcatctaa ttctactgaa gaagagccag acctttctaa
     3421 agaaacagaa aaagtcacta tagctgaaga gagccaagaa gctcttcctc agcaaaaagc
     3481 aaccacgaaa gagccacttc ttatcagtaa atctttagaa agtccttata tccccgacca
     3541 agctccaaaa tctagggata aatggaaaga gcaagtgctt gatttttggt cttggctagt
     3601 ggaagcgatc aaatctccta caagtaagtt ggaaacaagt atcacacaca gttacacagc
     3661 ctttctcttg ctcattctgt tttctgcatc ttcctttttc tttagtatct atcacatcaa
     3721 acatgcttac tatggacata tagcaagcat taacagtcgc ttccctgagc agctagctcc
     3781 tttaactctt ttttctatcg tctctatcct agtagcgaca acactcttct tcttttcatt
     3841 cctcttgggt agtttcgttg tgagacgatt tatccaccag gaaaaggact ggacgctaga
     3901 caaggttctc caacaatata gtcaactctt ggcaattcca atcttcctca ctgctattgc
     3961 tagtttcttt gctttctttg atagcctacg atttacagcc ctcttgtgtg tgattagcat
     4021 tggaatcatt ctgcttgcca gtctccatat cattacaaga ccaagtcaag caagtgaaac
     4081 cgactccttc tatcaattat tcttgtctgt ccttgtgaac ggagttatta tcctcctctt
     4141 ctttgtagct gaagtggcac tgattggaga ttatcttcgt atcttggcct ttctttaaaa
     4201 ttcaatcctg tcatccttgg caggattttt tctatgtcaa aaaagcaagt cgattgacct
     4261 gcttctgctt tacttttctt gtgctaatgt tttaaaatct tgtcctagga tgggttgtat
     4321 ttctatttga ttggcgtcta gttggtggta aaaagatggg ggtaatacct ctaagaattt
     4381 tgcatagtcc aagcgagcta tggattcata gtcttctggt ttagagccgt ctcctgtgat
     4441 ttgaaccaag tcaatctccc actggacttc cttatccacc aattgctcaa tataggctgc
     4501 aggaacaaac ggttctctcg gtaaaactgt cttgactcct tgagctagat ggtaaacgcc
     4561 atacactttt ccttctccat cctgatagaa ggaaactctc gcaaagtagg catctgtctc
     4621 ttgaaccgca cgcacacgcg cttcaaactg agccaagcca tctgcctcta aaaagctttt
     4681 caagttctca cgactaaaga aaataggatt aaaaattcct tggcaaatcg taaaacgagc
     4741 tacagggtct gctagatagg cttgaatact tgcttggaaa taagcttcaa aatcaaaacc
     4801 atctagccct tcaatatcag tccctgtata agcaagcaaa gcatctaaaa atgacttgat
     4861 aatctgccag tctgtctttg ttagtaggtc aggaagatcg acacggtaag ccttttgacc
     4921 atcagcataa ctgaccttaa agagaaattg tgattgcccc actatcgcac actcgatata
     4981 ttcgagacga ttaagaggct gacggagata gactacatca tagctatgtg actccaaacc
     5041 gtctagcaag gccaagatcg acttggcatt caaaatctct tgttctccta aaatgctctg
     5101 tttatttgga ataaaaaatg ttttcgccat aactggactc ctttgaaatc gtttacatac
     5161 agtatacctt atttttctga aagatacaaa aacaaacttt atactcttcg aaaatctctt
     5221 caaaccgcgt cagcttcgcc ttgccgtaga tatatgtaac tgacttcgtc agtcttatct
     5281 acaacctcaa agcagtgctt tgagcagcct gcggctagct tcctagtttg ctctttgatt
     5341 ttcattgagt attagatttt tgagtacaaa gcatagaaaa acagcccctg aggactgttt
     5401 gatcttatac agtagctgct tgatccaagc tttcaccgat agcggctagg cgctcaatca
     5461 cttcagcttg tgtcaattca ttttttgaaa catagcggtt acgtgggtga acacggcact
     5521 cgtgtgagca tccacgaagg tacttgtctt cattttcttc tgatgtcaag atacgacggt
     5581 tacagaaggg atttccacag ttgacataac gttcacatgg tgttccatca aaccagtctt
     5641 tccctacgat ggttgggttg acatggttga catcaacggc gatacgctcg tcaaatacgt
     5701 acatttttcc atcccaaagc tcaccttgaa cttctgggtc tttaccgtaa gttgcgattc
     5761 ctccgtgcaa ttggccgaca tctttgtagc cttcacggac catccagcct gagaatttct
     5821 cacagcgaac gccacctgta cagtaaacca cgacacgctt gtccatgaat ttttccttgt
     5881 tatcacggac ccattgtggt aactcacgga agttgcgaat atctgggcga atagctccac
     5941 ggaaatgtcc taggtcgtac tcataatcgt tacgtgtgtc aaggacaacg gtatctttat
     6001 caagaagcgc ttctttgaac tcttttggag acaagtaagc acctgttgtt tcaagtgggt
     6061 tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg gacaatttct ttcttgtagc
     6121 gaacaaacat cttcttgaag gcttgttcat tttcttcgtc aatcttgaac cagagttctt
     6181 ccatacctgg gaggctgtga acgtagtcca tgtatttttg agttgtttca tagtcacctg
     6241 aaactgttcc gttaattccc tcgtcagcga ctaggatacg gcctttaagg ccgattgatt
     6301 tacagaaagc caagtggtct gcagcaaatt gctctgcatt ttcaattgga gtataaaggt
     6361 agtaaagtaa gacacgaata tcttttgcca taagatttgt tctcttttct attcttaaat
     6421 tttctaaatt ttttatttat ccatactagt atacctgctt gagaaaacga atgcaattta
     6481 tttgactgga aattgtagag gattctcgaa atttcctgaa tggttaaaat aaggacaaga
     6541 gaaaacaggg atatctatat ccttgtgcca aaaaaaccac tgccctcccc agaccaacct
     6601 gaggaaagca gtgattctta ttttaggagt taggaatgaa tacacgaaat caatttagct
     6661 gattattttt tgtttttcaa gaattcatcg tattgttttt gcatttcgtt caatactttt
     6721 tcgtaggcac cttcagattt caatttttcc atcaattctg gaatcgcttt atctgggtct
     6781 acagtaccag tgttgatagc tgtatcaaat tgttgcattg tgttagcaat agctgagatt
     6841 tcagatttca cattgtcagt attgaagata aatccaagcg ctggagattc tttagcttct
     6901 gccaattctt tcttagaatt ttcgatttgt tggtctgtaa cgttttcgtt gatgtaaagg
     6961 atccagttgt taccagtgtt ccatccaccc atgtgagtgt ttcctttgta gccatcaaga
     7021 acgcgaacac ggttttcttt accttcaatt ttttcccagt tcttgccttc tggaccgtaa
     7081 acaagaccgt tcaagagttc tggattcgta ttcaagaggt tcaagatttc cattgatttt
     7141 tctttgttct tagagttgtt tgagatgaca aagttagcaa cttgtgttgt ttggtttttc
     7201 ttgatgaagt tagtaattgg tttgatttgg atatctttgt tggcaacacg tgaaagcaag
     7261 ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa cgaaccaagt atcttgttga
     7321 aggtcaaagg aagtatcgct tgttgcgacg tcttttggaa tgtagccagc ttcatagaat
     7381 ttgtgaagag tcttcaagtg ttctttgaaa cgaggcactt cgtaacggtt tacaacttta
     7441 gtagtatcgc cttcaaggtc aataacgaat ggaagcccgt ttgctactgg gtagtcaaaa
     7501 ttatcagatg ggatgaaaac tttaccaata gcaaatggta ctacgtctgg agctttttct
     7561 ttgatttgtt tcaagactgg ctcaagagtt tcgtaagaag taacacctga aatatcgata
     7621 ccatatttgg caaggagagt tccgttgaag gcaaagtttt gagatgatgc aacgttggct
     7681 gcaactggta cagcataaat cttaccattt acagtattac ccttgatata agctgggtca
     7741 agtgccttgt aaaggtcttt accttctttt ttgtacaatt ctgtcaagtc agcataagca
     7801 cctttttgag catttacaat atagttatct gcaaaggcga tatcatagtt ttcaccagat
     7861 gatgtgataa ctgacatttt cttaccatag tcaccccagc caaggtattg gatatccaat
     7921 ttagcaccaa ctttttcttc gatgattttg ttggcatttg ctaacaattc atccaagtta
     7981 tctggtttgt caccgatttg gtacattttg ataacaggtt tgtcacctga atcagcagct
     8041 tttttactgt tacctgtcaa gttgccacaa gcagcaagac ctgcagccaa agcgactaca
     8101 ctagcagatg caaaagcata ttttttccag tttttcatga taaaaactcc tttttttatt
     8161 tttaaactta taaacaatgt aatgatctta acttcacgca agggaagttt ggaaatgaga
     8221 aatgttcttt ttcaataagc actattcttt cacaccaccg atagtcaaac cttttacaaa
     8281 gtagcgttgg aagaatggat acaaaatcgc gattggaagg gttgctacca caaccatggc
     8341 catacgacct gtttctttcg gtagagcaac tcccagttga ccagtcaagc cgaccgcttt
     8401 ggcaatgtag tccatatttt gttggatttg catgagcaaa tattgcaatg gatacaagtt
     8461 atcactcttg atgtaaagaa gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa
     8521 aagcgtgatg gttgcgatac ctggtagtga caatggcaaa cagatttgga agaaaatccg
     8581 agcctcactg gcaccatcga tacgagccga ttcgagaatg gcttctggaa tggtcttctt
     8641 gaaaaaagaa cgcatcaaga tgatattgaa tggtgagaga agcattggaa caatcaaggc
     8701 ccaaacagtg tcaccaagct gaagtacacg ggtcaccatg atataacctg gtaccaaacc
     8761 agcgttgaac aacatactga gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt
     8821 tgtccgtgaa atagcgtagg cataggttgt tgtgataaag acatttgtca atgtcccaac
     8881 tacggttaca aagacagaga tgaagagggc ttgtaggatt ttatccttaa actgtgccaa
     8941 aaactcaaaa ccgtctaagc caaattggga tgggaagaag ctatagccgt attggaggag
     9001 gcttttctcg tctgtcactg aaataatgat aacgaataca aaaggtagga tacaagagag
     9061 ggcaatcaaa cccgaaataa tactgaagaa gatatctgct ttcttactga aggagtgaat
     9121 gccgacatta tcaatttttt cttttttaat tttctttttt gccatattct cctcctttct
     9181 agaacaaagc tgagtttgga tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa
     9241 tcaaaccaac aacggattgg taaagaccgg ctgctgcagc cataccgata tctgctgtct
     9301 gagtcaaacc attaaagaca tatacgtcca aaacgttggt tacattgtaa agctgaccag
     9361 cattgtgtgg gatttgatag aagagaccga agtctgcgcg gaagatattt ccgactgcaa
     9421 ggatggtcaa tacagttaca agcggagtca actgaggaat ggttacgttg cgaatacgtt
     9481 gccacttgct agctccgtcc actgtcgctg cttcgtagta ggttggatca attcccatga
     9541 tcgtcgcata gtacatgaca ctgctatatc caaagccttt ccaaatacct aggaaaagta
     9601 ggagataggg ccagatgccc aggtcagcgt agaaattgac ttctttgaga ccaagacttt
     9661 ccaatagatg attgaacacc cctttatcaa tatttaggaa ggcatctgta aagaaactaa
     9721 tgataaccca agacaagaag taagggaaca acatagaagt ttgaaaaatc ttcaccattc
     9781 tcttagaacg gagctcgctg aggataatgg caatccctac agatacaact aaacctagaa
     9841 agataaagcc aagattgtag aggacagtat ttcgtgtgat aataaaggcg tctcttgaac
     9901 taaataagaa tctgaaatta tcgagtccga cccatttact atttatgata ctatctatga
     9961 aaccattact ggtcatgtgg tagtctttga aggcaaccac gttcccaaat actggaatgt
    10021 aaaagaatag aatcaaccag agtgcccctg gcaaaaccat caagagaaag atccagttgt
    10081 ctctcaatgt ttttgaaaac tttttcataa tttcctccct ttttattttg atatccatct
    10141 aaaaattctt ttttagactt ttgataacga ttacattatt agtatactcc tatttgcagg
    10201 ttaggttaaa ctcctaatta tagaaaaaac tccacaaatt atgtagcaga tttaaaactt
    10261 tatcaccact atcaaacaaa tgtcctaaat caattgttta ttttatctct attagcccag
    10321 tgatggcgtc actctgttat aagcatccaa caacggggta tactgaaaaa tctccagact
    10381 agggaactca gagatagttc ctaatctgga gatttttaat atgttattag gcgtttgctt
    10441 tcaacttagc aataacctct ttaagattat caatcacctc tgcttcagta tgctcagagc
    10501 ctttttcatc tgccaagaac aaaactgctt tttgaagttc tttttgagag ttttcaagga
    10561 catccttatc tactgtttca aggtttgagt ctttaagaag tttacttaat tccttggcta
    10621 atttcttgag tttgatttgc agactcatct tctcctgctg tttctttgcc cgctgtttgt
    10681 cctccatcct tagttgctga ctggctttcc ttaatggact ctagggaagc aatggcatct
    10741 ttgactgttt gcaagatatc acgtaaacct tgctctgtca aactatcatc tgcaaaagct
    10801 ttcttagcct ctgccaaaac cagacgtgct gaatctgtgg taggattcga tacacctgtc
    10861 aatgatctca aaagattttc taaggtttga gtctgcttac taatactaga ctaaaatcaa
    10921 aaagcattat ataacagtga tatgaaatca actaaagaag aaatccaaac tatcaaaaca
    10981 cttttaaaag actctcgtac agctaaatat cataaacgcc ttcaaatcgt tctatagtaa
    11041 aatgaaacaa ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat
    11101 ttttggttca ttttactata tcactttgaa ctatatcggt ctagttatgt ctaaacgatt
    11161 atagctggat actaagagaa atcaaaaaag cactaaggga gaacgtccca aagtgcttaa
    11221 tatcaaggcc tcaaagccta tcttattcaa taaaatatta caacattttg ttgtagaatt
    11281 caacgacaag tgcttcgttg atttctgggt tgatttcgtc gcgttctggc aagcgagtca
    11341 atgaaccttc caatttttca gcgtcgaatg atacgaatgc tggacgtcca agagtagctt
    11401 ctactgcttc aaggattgct ggaactttca atgatttttc acgaactgag atcacttgac
    11461 ctggagttac gcggtatgat gggatatcaa cgcgtttccc gtcaacaagg atgtgaccgt
    11521 ggtttacgaa ttgacgagct tgacgacgag tagtcgcgag accaagacgg taaacaacat
    11581 tatccaaacg acgttccaaa agaagcataa agttgaaacc taggattcca cctttgattt
    11641 ttgtagcttg tacgaacaag ttacggaatt gtttttcacc tacaccgtaa gtgaaacgaa
    11701 gtttttgttt ttcagccaat tgcaaaccgt attctgacaa tttagaacgg ttgtttggtc
    11761 cgtgttgtcc tggtacgtag ttacgacgtg ccaattcttt acctgtacct gtaagtgaaa
    11821 ggccaaggcg acgagcttgt ttccaagatg gtcctgtata acgtgacata tgaatgtcct
    11881 cctgatataa ataatatttc ggtggaaata gtcacttaga aagccctgat tcgtgcagat
    11941 gcccttcgcc taaacagcca aggttacttg tcataagaca cctgttgacg agcttcatgc
    12001 tttcctgctg atatttcaca caaaatccat tttaccatga atagctggat ttgtaaaggg
    12061 gttttaggct ttattttcac taccagagag gttgagaacg agggtaaagg tacttcctag
    12121 accgtactgg ctgctgactg tgatttcccc acccaattga tgggccaatt cacgcgcaat
    12181 cgcaagtcct aatccatgac cacctgtctt catgttacgc gaagtttcga cacgataaag
    12241 acgtttgaaa atattttcca aatcctctgg ggcaatgccc tgcccttcat cggtcacact
    12301 gattgaaagc tggcccttct ccagcttagt caccacttcc agcttggttc ctggagcaga
    12361 atatttaaaa gcgttatcga ccagattcac caaaatacga gaaagcttag cataatctcc
    12421 ctcaatccgg gcagactctg ggattacctg caagtggaca tctcttctct cctgctcaat
    12481 caaaaactga aattcactca tgcactcaat taagagcttg tccagaaaaa tactgtcttt
    12541 actggtagtt tccacctgat ttctagctgt atttagggtc aaaaaattca actcctcaac
    12601 cagtttattg agcctctccg tctggcgtcc aatggttgct agataatgag cttgctccga
    12661 ctccttgata atcccatcca aaatcccttc taccgtcgct tggatcgaag tgataggagt
    12721 cttaatatca tgcgacaact gggcaatcat caagcccttt tctcgttcgc tttcttccaa
    12781 ggaatcaaag cttacctgca aatcatggga catctcatta aaagtttgcc ctaattgctg
    12841 aaattctaca ggaccttgaa cctccaaatt tgaaggaaaa tccttggccg ctacccgctt
    12901 ggcatgctcc ttgagtttgc ccaacgacgt aaagactggc aataggagaa agagactaat
    12961 cccagcaccg acaaggctgg caacgatggt catcccaagc aaaaagtaaa tctcgccttt
    13021 cgcaatcagc attttttgaa cagcccaaaa aacaaccaaa atggttaaga gggttgaaat
    13081 aatatatcca accaaaatat aactttttag tttcatgttt gtcctctcgg tttctctatc
    13141 ttatatccca acccccaaac tgtcttaata gtgggcgttt ggtcactact atattttgcc
    13201 agctcctgtc gaagagcatg gatatgcaca ttcaaggtat tggtgtcatc cacgtagtct
    13261 tctttccaga tcttttcata gaggtctgtc ttggagaaaa ctcgctctgg attgctagct
    13321 aaaatccaca gcaattcaaa tgatttaaca gttaaatcca gcatttcttc tcctatttga
    13381 acttcatgac tactatgatt catttttaga ttgccaaggg aaatcagctc tgtttcgccc
    13441 ccacgatgaa ggcggcgcaa aatattgtgg acacgcaaaa ccagctcacg tgggctaaaa
    13501 ggcttagcaa taaaatcatc tgctcccaag ctcaggccgt aaatcttgtc ctgttcactg
    13561 gtcttagcag taataaatag gaaaggctgc tctggtgata agtattgaac ctcactgatt
    13621 aaatcataac catccatccg aggcatcatg acatctgtga taatcaaatc aatcggtttt
    13681 ttcttgaaca gctctagcgc ttccagtcca tcatgggcta ccaagacctg ataacctgcc
    13741 tgaattaagt atctctgatg aatatctgtg atttctacct cgtcgtcaac gagtaaaatt
    13801 gtctttccca tctgtctctc ctttgataaa aacagtgcta tactgcttta agtataacac
    13861 tatttttatt gttttacgat gattttaatc tgctattgaa agaaagtcct gattgctgat
    13921 tgagttgtta aactaatctt agtcagccca ctatccatca tttatttcct tagctgatta
    13981 gttttcttct tttttgtttt tcaaacctag accaccgagt aaacctgcaa gcgcaagccc
    14041 aaggaaacca atacttgcca ttgatgtttg agtctcacca gtatttggta gcatagcttt
    14101 atcctctgac atcatcgtat cagacatctt gttagcagaa gcagccatgt tttcacctgc
    14161 catcgtgttg gtagaacttg tcatggtgtc agcaggcatg ctatctgtag tacctgtagc
    14221 atgattgtga ttcatcggag tcacgccaga accagagtta gaaggtgata atgaaccatt
    14281 tgctgtgtct gatgtttctt taacatttat cttaatagtg acttttttag ttgctacgat
    14341 gttgtccaag tctggtttac cgtctttgtt accatagaca ttgactgtag cgctgtaagt
    14401 ttgagtaccg tttgctcgga actggtcaat gagcgcttgt ttttctttgc cagctacatt
    14461 tccgtccaag gctaattgat agaagtattg acctttggtc ttcacgtttt cgcctagtgg
    14521 agatagggct gggtttttag cgtcgccgtt atctgaccac ggtgcctt
//
LOCUS       DAVDWM010000048        14282 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVDWM010000048 DAVDWM010000000
VERSION     DAVDWM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.048"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..297
                     /locus_tag="VUW49_001745"
     CDS             <1..297
                     /locus_tag="VUW49_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981631.1"
                     /translation="SRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTVEDK
                     AYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(612..2303)
                     /locus_tag="VUW49_001746"
     CDS             complement(612..2303)
                     /locus_tag="VUW49_001746"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7981632.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFERVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2439..2885
                     /gene="argR"
                     /locus_tag="VUW49_001747"
     CDS             2439..2885
                     /gene="argR"
                     /locus_tag="VUW49_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET7981633.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2936..5470
                     /gene="mutS"
                     /locus_tag="VUW49_001748"
     CDS             2936..5470
                     /gene="mutS"
                     /locus_tag="VUW49_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7981634.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5617..7311
                     /gene="patA"
                     /locus_tag="VUW49_001749"
     CDS             5617..7311
                     /gene="patA"
                     /locus_tag="VUW49_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET7981635.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7642..7911)
                     /locus_tag="VUW49_001750"
                     /pseudo
     CDS             complement(7642..7911)
                     /locus_tag="VUW49_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            8103..9869
                     /gene="patB"
                     /locus_tag="VUW49_001751"
     CDS             8103..9869
                     /gene="patB"
                     /locus_tag="VUW49_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET7981636.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSAGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            10008..10697
                     /locus_tag="VUW49_001752"
     CDS             10008..10697
                     /locus_tag="VUW49_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7981637.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            10701..10880
                     /locus_tag="VUW49_001753"
     CDS             10701..10880
                     /locus_tag="VUW49_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981638.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            10867..12216
                     /locus_tag="VUW49_001754"
     CDS             10867..12216
                     /locus_tag="VUW49_001754"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET7981639.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            12340..13800
                     /locus_tag="VUW49_001755"
     CDS             12340..13800
                     /locus_tag="VUW49_001755"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7981640.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            13814..14248
                     /locus_tag="VUW49_001756"
     CDS             13814..14248
                     /locus_tag="VUW49_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7981641.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         4187 a         2767 c         3213 g         4115 t
ORIGIN      
        1 agtcgcagaa atatcggaca agctggtaaa atcttggctg acagtggtta tcaagggctc
       61 atgaagatat atcctcaagc acaaactcca cgtaaatcca gcaaactcaa gccgctaaca
      121 gttgaagata aagcctataa ccatgcgcta tccaaggaga gaagcaaggt cgagaacatc
      181 tttgccaaag taaaaacgtt taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc
      241 ttcggattac gaatgaattt gattgctggt attatcaatc atgaactagg attctagttt
      301 tgcaggaagt ctattatttg gttaggtgaa ttagtgaagc gtttaggcaa gtgtctctgg
      361 ttacgacgtc atggcctcta aatcgattat atttaggggt catgactagt gaagcagtta
      421 gctagttcgc atataagcgg ctagcgtcta acaattagga actttagttc caataacttt
      481 aagattacga cgttttagga cataaatcga tcatatttat gtcctaaaac tagtgaagcg
      541 cctagccaaa gtccgaatag gatttggcgt tagttactta gattgctttg caatcaagta
      601 actttggcga tttacatctt ctctggcgct tctactccaa gcaagcgaag ggcttctttg
      661 agaacgactg cggttgcgta gctgagggct agacggctgt cgcgttctgg gctttcatcc
      721 aagatacgtg tatgtgcata gtatttgtta aaggattgag ccaggctaat tgcaaattta
      781 gcaatgatag aaggttcaaa gttatctgcc gcacggttga taatacgtgg gaagtcttga
      841 atgagtttaa tgatttccca gctttcagta tcattcaagc tatagttgcc agctgtttct
      901 ggtttgaaat cggctttgcg taagatagat tggatacgag cgtaggcata ttgaacgtaa
      961 ggtccagttt caccctcgaa ggataccata gcttctaggt cgaagtcgta tccatttgta
     1021 cggtcggttt tgaggtcata gaatttaatg gctccaaccc caacagcatg tgctacttgg
     1081 tctttgtttt ctagttcagg atttttagcc tcgatttgga ccttggcacg gctaacagcc
     1141 tctgcaacag taggctctag caagatgaca ttccctttac gagtagagag cttcttccct
     1201 tcttttgtaa ccagaccaaa aggaacgtgg gtaatgtcgt cactccaatc gtagcccatc
     1261 tcttgcaaga cagctttgag ctgtttaaag tgggcagatt gttcttgacc aacgacatag
     1321 atagatttag caaattggta ttcgttttta cggtagaggg ctgcagccaa gtcacgtgtg
     1381 atatagagag ttgcaccatc agacttcttg atgagggctg gatgttcaat tccatatttc
     1441 tcaagattca caacttgggc accttctgat tcaagaagta gtcctttttc agaaagaatg
     1501 tctacaactg catccatctt atcattgtag aaggcttctc cgttatagct gtcaaattca
     1561 accttcaatt cattgtaaag gcggttaaat tccactaaac tttcatcgcg gaaccattgc
     1621 caaagagcga gagcttcctc atctccattt tcaagtttac ggaaccattc gcgcgcttct
     1681 tcatccaagc tagggtcatt ttcagcttca gcgttgatgc ggacatagag tttaaggagt
     1741 tcatcgattg gatgagcttt tacagcttct tcgtcgcccc attttttgta ggcaacaatc
     1801 aacatcccaa attgtttacc ccagtctccc aaatggttga ccttgaccgt ttgataaccg
     1861 attttttgga aaatatgtga caagctatct ccgataacag ttgaacgcag gtggccaata
     1921 gaaaatggtt tagcgatatt cggactagac atgtcgataa caacattttc ttgtttacca
     1981 atattttggt cagcatagtg ttctttttca gtggtaacag cttgcaatac ttgagcagaa
     2041 atggcagatt tatcaaggaa aaagttaacg taaggtcctg ttgcgacaac tctttcaaag
     2101 gcttggctgt tcattttttc agccagttca gccgcaatca tttgtggtgc tttacgttcg
     2161 actttggcaa gagaaaaagc agggaaagcg atgtcaccca tttctgagtt tttaggggtt
     2221 tccagtaact ttaaaatagc ctcttggtcc aggctatcaa tgatgctagc caattcgcta
     2281 gcaatcaatt cttttgtatt cattaagagc tcctttttgg acttttctac tattttaaca
     2341 caattttaaa gaaagaagaa aaaatttttg aaatctcctg tttttttggt ataatatggt
     2401 tataaatata gttataaata tgcacgcaag aggattttat gagaaaaaga gatcgtcatc
     2461 agttaataaa aaaaatgatt actgaggaga aattaagtac acaaaaagaa attcaagatc
     2521 ggttggaggc gcacaatgtt tgtgtgacgc agacaacctt gtctcgtgat ttgcgcgaaa
     2581 tcggcttgac caaggtcaag aaaaatgata tggtgtatta tgtactagta aatgagacag
     2641 aaaagattga tttggtggaa tttttgtctc atcatttaga aggtgttgca agagcagagt
     2701 ttaccttggt gcttcatacc aaattgggag aagcctctgt tttggcaaat attgtagatg
     2761 taaacaagga tgaatggatt ttaggaacag ttgctggtgc caatacctta ttggttattt
     2821 gtcgagatca gcacgttgcc aaactcatgg aagatcgttt gctagatttg atgaaagata
     2881 agtaaggtct tgggagttgc tctcaagact tatttttgaa aaggagagac agaaaatggc
     2941 gatagaaaag ctatcacccg gcatgcaaca gtatgtggat attaaaaagc aatatccaga
     3001 tgcttttttg ctctttcgga tgggtgattt ttatgaatta ttttatgagg atgcggtcaa
     3061 tgctgcgcag attctggaaa tttccttaac gagtcgcaac aagaatgccg acaatccaat
     3121 ccctatggcg ggtgttccct atcattctgc ccaacagtat atcgatgtct tgattgagca
     3181 gggttataag gtggctatcg cagagcagat ggaagatcct aaacaagcag ttggggttgt
     3241 taaacgagag gttgttcagg tcattacgcc agggacagtg gtcgatagca gtaagccgga
     3301 cagtcagaat aattttttgg tttccataga ctgcgaaggc aatcaatttg gcctagctta
     3361 tatggatttg gtgacgggtg acttttatgt gacaggtctt ttggatttca cgctggtttg
     3421 tggggaaatc cgtaacctca aggctcgaga agtggtgttg ggttatgact tgtctgagga
     3481 agaagaacaa atcctcagcc gccagatgaa tctggtactc tcttatgaaa aagaaagctt
     3541 tgaagacctt catttattgg atttgcgatt ggcaacggtg gagcaaacgg catctagtaa
     3601 gctgctccag tatgttcatc ggactcagat gagggaattg aaccacctca aacctgttat
     3661 ccgctacgaa attaaggatt tcttgcagat ggattatgcg accaaggcta gtctggattt
     3721 ggttgagaat gctcgctcag gtaagaaaca aggcagtctt ttctggcttt tggatgaaac
     3781 caaaacggct atggggatgc gtctcttgcg ttcttggatt catcgcccct tgattgataa
     3841 ggaacgaatc gtccaacgtc aagaagtagt gcaggtcttt ctcgaccatt tctttgagcg
     3901 tagtgacttg acagacagtc tcaagggtgt ttatgacatt gagcgcttgg ctagtcgtgt
     3961 ttcttttggc aaaaccaatc caaaggatct cttgcagttg gcgactacct tgtctagtgt
     4021 gccacggatt cgtgcgattt tagaagggat ggagcaacct actttagcct atctcatcgc
     4081 acaactggat gcaatccctg agttggagag tttgattagc gcagcgattg ctcctgaagc
     4141 tcctcatgtg attacagatg ggggaattat ccggactgga tttgatgaga ctttagacaa
     4201 gtatcgttgc gttctcagag aagggactag ctggattgct gagattgagg ctaaggagcg
     4261 agaaaactct ggtatcagca cgctcaagat tgactacaat aaaaaggatg gctactattt
     4321 tcatgtgacc aattcgcaac taggaaatgt gccagcccac tttttccgca aggcgacgct
     4381 gaaaaactca gaacgctttg gaaccgaaga attagcccgt atcgagggag atatgcttga
     4441 ggcgcgtgag aagtcagcca acctcgaata cgaaatattt atgcgcattc gtgaagaggt
     4501 cggcaagtac atccagcgtt tacaagctct agcccaagga attgcgacgg ttgatgtctt
     4561 acagagtctg gcggttgtgg ctgaaaccca gcatttgatt cgacctgagt ttggtgacga
     4621 ttcacaaatt gatatccgga aagggcgcca tgctgtcgtt gaaaaggtta tgggggctca
     4681 gacctatatt ccaaatacga ttcagatggc agaagatacc agtattcaac tggttacagg
     4741 gccaaacatg agtgggaagt ctacctatat gcgtcagtta gccatgacgg cggttatggc
     4801 ccagctgggt tcctatgttc ctgctgaaag cgcccattta ccgatttttg atgcgatttt
     4861 tacccgtatc ggagcagcag atgacttggt ttcgggtcag tcaaccttta tggtggagat
     4921 gatggaggcc aataatgcca tttcgcatgc gaccaagaac tctctcattc tctttgatga
     4981 attgggacgt ggaactgcaa cttatgacgg gatggctctt gctcagtcca tcatcgaata
     5041 tatccatgag cacatcggag ctaagaccct ctttgcgacc cactaccatg agttgactag
     5101 tctggagtct agtttacaac acttggtcaa tgtccacgtg gcaactttgg agcaggatgg
     5161 gcaggtcacc ttccttcaca agattgaacc gggaccagct gataaatcct acggtatcca
     5221 tgttgccaag attgctggct tgccagcaga ccttttagca agggcggata agattttgac
     5281 tcagctagag aatcaaggaa cagagagtcc tcctcccatg agacaaacta gtgctgtcac
     5341 tgaacagatt tcactctttg atagggcaga agagcatcct atcctagcag aattagctaa
     5401 actggatgtg tataatatga cacctatgca ggttatgaat gtcttagtag agttaaaaca
     5461 gaaactataa aaccaagact cactagttaa tctagctgta tcaaggagac ttctttgaca
     5521 attctccact tttttgctag aataacatca cacaaacaga atgaaaagga gctgacgcat
     5581 tgtcgctccc ttttgtctat tttttaagga gaaagtatgc tgattcagaa aataaaaacc
     5641 tacaagtggc aggccttggc ttcgctcctg atgacaggct tgatggttgc tagttcactt
     5701 ctgcaaccgc gttatctgca ggaagtatta gacgccctcc ttactgggaa atatgaagct
     5761 atttatagta tcggggcttg gttgattggt gtggccgtag tcggtctagt tgctggtgga
     5821 ctcaatgttg tcctcgcagc ctatattgcc caaggagttt catccgacct tcgggaggat
     5881 gccttccgta aaattcaaac cttttcttat gctgatattg aacaatttaa tgcgggaaat
     5941 ctagtcgttc gaatgacaaa tgatatcaac cagattcaga acgttgtcat gatgaccttc
     6001 caaattcttt tcagacttcc cctcttgttc atcggttcgt ttatcctagc ggttcaaacc
     6061 ttaccttctc tgtggtgggt gattgttctc atggtagtct tgatttttgg tttgactgct
     6121 gtcatgatgg gaatgatggg gcctcgtttt gccaagtttc aaacccttct tgagcgcatc
     6181 aatgccattg ctaaggaaaa tttacgtggc gttcgtgtgg tcaagtcctt tgtccaagaa
     6241 aaagagcaat ttgctaagtt tacagaggtc tcagacgagc tttttggtca aaacctttac
     6301 attggttatg ccttttcagt agtggaaccc tttatgatgt tggttggtta cggggcggtc
     6361 ttcctctcta tttggctggt cgcgggaatg gttcagtcgg atccgtctgt tgttggttcc
     6421 atcgcttctt ttgttaatta cctaagccag attatcttta ccattgttat ggttggattt
     6481 ttgggaaatt ctgtcagccg tgccatgatt tccatgcgtc gtattcgaga aattcttgac
     6541 gcagagccag ctatgacctt caaggatatc ccagatgaag agttggttgg aagccttagc
     6601 tttgaaaatg tgacctttac ctatccaatg gacaaggaac cgatgctgaa agatgtgagc
     6661 tttactattg aacctggtca aatggttggt gtagttggag cgactggtgc aggaaagtca
     6721 accttggctc aattgattcc acgtctcttt gatccacaga acggggccat taaaatcggt
     6781 ggcaaggata ttcgagaagt gagtgaagga accctgcgta aaacagtttc catcgttctc
     6841 caacgtgcca ttctttttag tggaacgatt gcagataact tgagacaggg gaagggaaat
     6901 gctactctat ttgaaatgga gcgcgcagcc aatattgccc aggctagtga attcattcat
     6961 cgtatggaga aaacctttga aagtccagtt gaagaacggg gaaccaattt ctctggtgga
     7021 caaaaacaaa ggatgtcgat tgcgcgtggg attgtcagca atccacgtat tctgattttt
     7081 gatgattcga cctcagcctt ggatgccaaa tcagagcgct tggtgcaaga agctttgaat
     7141 aaggacttga agggtacgac aaccattatt attgctcaaa aaattagctc ggttgtccat
     7201 gcagacaaga tcttggttct aaatcaagga cgattgattg gtcaaggcac gcatgcagac
     7261 ttggttgcca acaatgccgt ttaccgtgaa atctatgaaa cacagaaatg aaagacaaac
     7321 tataagaaaa gtcaatagtt ttatctaaac tatttcttat ttcaatttga tgatttggcg
     7381 atgattttag agaacggcaa aaagcccttg aaaaagtcca ttttttcaaa ggtaatcctg
     7441 tgttgatttc agaaattaca tcactttttg ttcgtcaaat ggcagctctt tttttaggat
     7501 ataaaacagg gttcggataa gtttttttgc aaggtggatg atggctacat tgtaatgttt
     7561 tccttgttct aatttagtct taagataggc cttaaaagca ggcgaaaagc gagggcatgc
     7621 tttggcagct tgtatgagta cctaccgcag atgaggggaa ctccgtttga ccattcttcc
     7681 tgctaaatca atctgatctg actgataaat agaagaatcc agtccagcga aagcttgtaa
     7741 ttgagcagga ttatcaaagg catgaatatt tcgaatctca gctaaaatga ccgcccctaa
     7801 acgatcccca atcccagtaa ccgtcgtgat gaccgagttg aactcagcca tcaagtcatt
     7861 gacacatttt tccgccttgt caatgagcct cttgtaatgt ttgatgtttt cattacacga
     7921 gataaaacgt ttatgcgtta tcaaactcat gaccaattga aacgaaaagc tgtggttaga
     7981 tcctttcgga aattgtcaag cgattggagg aaatgaacta atccacagcg gcttattcca
     8041 agtataccac ttgggctttg gcagtagcta actgcgctaa atataatata aggaggagta
     8101 aaatgaagac agttcaattt ttttggcatt attttaaggt ctacaagttc tcatttgtag
     8161 ttgtcatcct gatgattgtt ctggcgactt ttgcccaagc cctctttcca gtcttttctg
     8221 gacaagcggt gacgcagcta gccaatttag ttcaagctta tcaaaatggc aatccagaac
     8281 ttgtatggca aagcctatca ggaatcatgg tcaatcttgg cctgctggtt ttggttctat
     8341 ttatctctag tgtaatatac atgtgtctca tgacgcgcgt gattgcagaa tcgaccaacg
     8401 agatgcgcaa aggcctcttt ggtaagcttg ctcagttgac ggtttctttc tttgaccgtc
     8461 gacaagatgg cgatatcctg tctcatttta ccagtgattt ggataatatc ctccaagcct
     8521 ttaacgaaag cttgattcag gtcatgagca atattgtttt atacattggt ctgattcttg
     8581 tcatgttttc gagaaatgtg acgctggctc tcatcaccat tgccagcacc ccattggctt
     8641 tccttatgct gattttcatc gtgaaaatgg cacgtaaata caccaacctc cagcagaaag
     8701 aggtagggaa gctcaacgcc tatatggatg agagcatctc aggccaaaaa gccgtgattg
     8761 tgcaaggaat tcaagaggat atgatggcag gatttcttga acaaaatgag cgcgtgcgca
     8821 aggcaacctt taaaggaaga atgttctcag gaattctttt ccctgtcatg aatgggatga
     8881 gcctgattaa tacagccatc gtcatctttg ctggttcggc tgtacttttg aatgataagt
     8941 ctattgaaac aagtacagcc ctaggtttga ttgttatgtt tgcacaattt tcacagcagt
     9001 actaccagcc tattatccaa gttgcagcga gttggggaag ccttcagttg gcctttactg
     9061 gagctgaacg aattcaggaa atgtttgatg cagaggagga aatccgacct gaaaaggctc
     9121 caaccttcac taagttgcaa gaaagtgttg aaatcagtca tatcgatttt tcatacttgc
     9181 ctgataaacc tattttgaaa gatgtcagca tttctgcccc taaaggccag atgacagcag
     9241 ttgttgggcc gacaggttca ggaaaaacga ctattatgaa cctcatcaat cgcttttatg
     9301 atgttgatgc tggtggtatt tattttgatg gtaaagacat tcgtggctat gacttagata
     9361 gtcttagaag caaggtggga attgtattgc aagattcggt cttgtttagc ggaacgattc
     9421 gagacaatat ccgatttggt gtgccagatg ctagtcagga aatggttgag gtagcagcaa
     9481 aagcaaccca cattcacgac tatatcgaaa gtttgcctga taagtacgat actcttattg
     9541 atgatgacca gagcatcttt tcagcagggc agaagcaatt gatttcaatc gctcgaaccc
     9601 tgatgacaga tccagaagtt ctcattctcg atgaagcaac ttcaaacgta gatacggtga
     9661 cagaaagcaa gattcaacat gccatggagg tggttgtagc aggtagaact agtttcgtca
     9721 ttgcccaccg cttgaaaacc attctcaatg cagatcagat tattgtcctt aaagatggag
     9781 aagtcattga acgtggtaac caccatgaac ttttgaagct aggtggcttt tattcagaac
     9841 tctatcacaa tcaatttgtt ttcgaataag aaagaagttg tcctatgtgg gcagcttttt
     9901 cttgtccata aaaaatgttt atcacagcct taaaaaaaac atattagacg aaagtcattt
     9961 tgagtgatat gataggacta tcgttagcat tcgaaaggag aggcatcatg gctagaacgg
    10021 ttgtaggagt tgctgcaaat ctatgtcccg tagacgcaga aggcaaaatc attcattcat
    10081 ctgtatcttg tagatttgca gagatcattc gtcaagtcgg tggtctccct ttagtcattc
    10141 ctgttggtga tgagtcagtt gtacgtgatt atgtggaaat gattgacaaa ctcattttga
    10201 caggaggcca aaatgttcat cctcagtttt atggagagaa aaagaccgtc gagagcgatg
    10261 attacaatct ggtccgtgac gaatttgaat tggcactctt gaaggaagcg cttcgtcaga
    10321 ataaaccaat tatggcaatc tgtcgcggtg tccaacttgt caatgttgcc tttggtggaa
    10381 ccctcaatca agaaatcgaa ggtcactggc aaggactacc tttcgggaca tctcactcta
    10441 ttgagacagt agaaggaagc gtggtggcta agctatttgg gaaagaaagt caggtcaact
    10501 cagtccatcg tcaaagcatt aaagatttgg cacctaattt ccgtgtaact gctattgatt
    10561 caagagacca aactattgaa gcgattgagt ctatcgacga gcaccgcatt atcggtttac
    10621 agtggcatcc agagtttctg gttaatgaag aagatggcaa tttagaatta tttgagtatt
    10681 tattgaatga actgtaacga ttggaacatc tggtcgttct ttcttttatt ttttcaaaat
    10741 ttttagaatg tggcatttta cgcaaacgtt tgaattctga taaaaattgg agaaattcga
    10801 caaaaaaact tgaaaaaaac gaaggtaagc gttatgatag aaaagaagaa atattggagg
    10861 aagaacatgt cacatattaa atttgattat tcaaaagttt tagacaaatt tgttgcacca
    10921 catgaagtgg aatacatgca atcacaagta acagcagcag atgaattgat ccgtaaagga
    10981 actggtgctg gtagcgactt cttgggatgg ttggaccttc ctgaaaaata cgaccgcgaa
    11041 gaatttgacc gcatcttgaa agctgctgag caaatcaagt cagacagcga tgttttggtt
    11101 gtgatcggta ttggtggatc ttaccttggt gcaaaagcag caatcgactt cttgaatcac
    11161 cattttgcta acttgcaaac aaaagaagaa cgcaaagctc cacaaatcct ttacgcagga
    11221 aactcaatct catctactta ccttgctgac ttggtagagt atgttgcaga caaagacttc
    11281 tcagtaaacg taatttctaa atcaggtaca acaactgaac cagcgattgc tttccgtgtc
    11341 tttaaagaac tcttggttaa gaaatacggt caagaagaag ctaacaaacg tatctatgca
    11401 acaactgacc gccaaaaggg tgctgttaag gttgaagcag acgctaacgg ttggggaaca
    11461 tttgttgttc cagatgatat cggtggacgc ttctcagtat tgacagccgt tggtttgctt
    11521 ccaatcgcag catcaggagc tgacatcaaa gctcttatgg aaggtgcgaa tgcagctcgc
    11581 aaagactaca cttcagacaa aatctctgaa aacgaagctt accaatacgc agctgttcgt
    11641 aacatccttt atcgtaaagg ctatgcaact gagatcttgg taaactatga gccatcactt
    11701 caatacttct cagaatggtg gaaacaattg gctggtgaat cagaaggaaa agaccaaaaa
    11761 ggtatctacc caacttcagc caacttctca actgacttgc actcacttgg tcaatttatc
    11821 caagaaggaa ctcgtatcat gtttgaaaca gttgtccgtg ttgacaaacc tcgtaaaaac
    11881 gtgcttattc ctactttgga agaagacctt gacggacttg gttaccttca aggaaaagac
    11941 gttgactttg taaacaaaaa agcaactgac ggtgttcttc ttgcccacac agatggtgat
    12001 gtaccaaaca tgtatgtgac tcttccagag caagacgctt tcactcttgg ttacactatc
    12061 tacttcttcg aattggcaat tgccctttca ggttacttga atgctatcaa cccatttgac
    12121 caaccaggtg ttgaagctta taaacgtaac atgtttgccc ttcttggaaa accaggattt
    12181 gaagaattga gcaaagaact taacgcacgt ctataataga agaaaagagt ggtttgccca
    12241 ctctttttac tctctttatc catagaaatt ggactcagcc aagacttgtg atataatata
    12301 gaaagcaaaa aggcagacgc ctagataata ggagaaacta tgtcaaaaga tatccgcgta
    12361 cgttacgcac caagtccaac aggactacta cacatcggaa atgctcgtac agcattgttt
    12421 aattacttgt atgcgcgcca tcatggtgga acatttctca tccgtatcga agatactgac
    12481 cgtaaacgcc atgtcgagga tggtgaacgt tcacaacttg aaaaccttcg ctggttaggc
    12541 atggattggg atgaaagtcc agaatcacat gagaattatc gccagtctga gcgtttggac
    12601 ttgtatcaaa aatacattga ccaactatta gctgaaggaa aagcctataa atcttacgtt
    12661 acagaagaag agttggcagc tgaacgcgaa cgccaagaag tagctggcga aacaccacgc
    12721 tacatcaatg aataccttgg tatgagtgaa gaagaaaaag cagcttacat cgcagaacgt
    12781 gaagcagcag ggatcatccc aactgttcgt ttggctgtca atgagtcagg tatctacaag
    12841 tggcatgata tggtcaaagg cgatatcgaa tttgaaggtg gcaatatcgg tggtgactgg
    12901 attatccaaa agaaagacgg ttacccaact tacaactttg ccgttgccat cgatgaccat
    12961 gatatgcaaa tctctcatgt tatccgtgga gacgaccata ttgctaacac accaaaacag
    13021 ctcatggttt atgaagcact tggttgggaa gctccagagt ttggtcacat gaccttgatt
    13081 atcaactctg agactggtaa gaagttatct aaacgtgata ctaatacact tcaatttatc
    13141 gaagattacc gaaagaaagg ttaccttcca gaagcagtct ttaactttat tgctcttctt
    13201 ggttggaacc caggtggtga agatgagatt ttctctcgtg aagaactcat taaacttttt
    13261 gatgaaaacc gcctcagcaa gtcaccagca gcctttgatc agaaaaaact agactggatg
    13321 agcaatgatt atatcaagaa tgcagacctt gaaaccatct ttgaaatggc aaaaccattc
    13381 ttagaggaag caggccgttt gactgacaag gctgaaaaat tagttgagct ctataaacca
    13441 caaatgaaat cagtagatga gattatccca ttgacagatc ttttcttctc agatttccca
    13501 gaattgacag aagcagagcg cgaagtcatg acgggtgaaa cagttccaac agttcttgaa
    13561 gcattcaaag caaaacttga agcgatgaca gatgataaat ttgtgacaga aaatatcttc
    13621 ccacaaatta aagcagttca aaaagaaaca ggtattaaag ggaaaaatct tttcatgcct
    13681 attcgtatcg cagtttcagg cgaaatgcat gggccagaat taccagatac aattttcttg
    13741 cttggacgtg aaaaatcaat tcagcatatc gaaaacatgc taaaagaaat ctctaaataa
    13801 agaggataca ataatggaca tctgggaaaa gatgtacgaa gaagcacaga aactatacaa
    13861 tccacatgaa gtatcagact ttgtttatgc caatcatgtt gtagctgcag tagaagcaga
    13921 agatggacaa atatttacag gattctgtat ggagggaacc tgtggtgttt tccatctctg
    13981 cgcagaacgg gcggccctct tcaatatgta ccaattttca ggacaaacta aggttaagaa
    14041 agtattagcc tttcgagaca aaccacctta tggtggaagt tcagccatgc cttgcggtgc
    14101 ttgtagagaa ttccttttag agcttaatgc tgaaaataaa gatgcagaat tcatgatgga
    14161 ctataatata agaaaaacag ttaaagtcgc agaactaatc ccttattggt ggggagaaga
    14221 acgtgcttct aagtttaatg agcgatagaa gagtcagtat aattctggct ctttttacta
    14281 ta
//
LOCUS       DAVDWM010000049        13995 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVDWM010000049 DAVDWM010000000
VERSION     DAVDWM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.049"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1..835
                     /locus_tag="VUW49_001757"
                     /pseudo
     CDS             1..835
                     /locus_tag="VUW49_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(970..2172)
                     /gene="rpsA"
                     /locus_tag="VUW49_001758"
     CDS             complement(970..2172)
                     /gene="rpsA"
                     /locus_tag="VUW49_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7981642.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2334..2405)
                     /locus_tag="VUW49_001759"
     tRNA            complement(2334..2405)
                     /locus_tag="VUW49_001759"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2371..2373),aa:Gln,seq:ttg)
     gene            complement(2415..2495)
                     /locus_tag="VUW49_001760"
     tRNA            complement(2415..2495)
                     /locus_tag="VUW49_001760"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2459..2461),aa:Tyr,seq:gta)
     gene            complement(2545..2775)
                     /locus_tag="VUW49_001761"
     CDS             complement(2545..2775)
                     /locus_tag="VUW49_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET7981643.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2943..3587)
                     /gene="pcp"
                     /locus_tag="VUW49_001762"
     CDS             complement(2943..3587)
                     /gene="pcp"
                     /locus_tag="VUW49_001762"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET7981644.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3602..4525)
                     /locus_tag="VUW49_001763"
     CDS             complement(3602..4525)
                     /locus_tag="VUW49_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET7981645.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4522..5211)
                     /locus_tag="VUW49_001764"
     CDS             complement(4522..5211)
                     /locus_tag="VUW49_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET7981646.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5280..5942)
                     /locus_tag="VUW49_001765"
     CDS             complement(5280..5942)
                     /locus_tag="VUW49_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7981647.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPAPYLNIMDPKSGSAMKHLG
                     ITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMPTASS
                     GGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQIPKRTRK
                     IH"
     gene            complement(5998..7020)
                     /locus_tag="VUW49_001766"
     CDS             complement(5998..7020)
                     /locus_tag="VUW49_001766"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET7981648.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7150..9621)
                     /gene="parC"
                     /locus_tag="VUW49_001767"
     CDS             complement(7150..9621)
                     /gene="parC"
                     /locus_tag="VUW49_001767"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET7981649.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDVAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKNFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(10041..11984)
                     /gene="parE"
                     /locus_tag="VUW49_001768"
     CDS             complement(10041..11984)
                     /gene="parE"
                     /locus_tag="VUW49_001768"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET7981650.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            12120..12761
                     /gene="plsY"
                     /locus_tag="VUW49_001769"
     CDS             12120..12761
                     /gene="plsY"
                     /locus_tag="VUW49_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7981651.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12806..13995)
                     /locus_tag="VUW49_001770"
                     /pseudo
     CDS             complement(12806..13995)
                     /locus_tag="VUW49_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4017 a         3136 c         2451 g         4391 t
ORIGIN      
        1 cactttgagc atgatttttc tcggcttcca aatattatgt cttgggatga gtatgccttc
       61 actaagggaa agatgagttt cattgcgcaa gattttgata atcttaatat tatcactgtt
      121 cttgaaggca gaacacaagc tgtcatccga aatcactttc ttcgctacga tagagccgtt
      181 cgttgtcaag tgaaaatcat tacgatggat atgtttagtc cttactatga cttggctaaa
      241 cagctttttc cgtgtgctaa aatcgttcta gatcgtttcc atattatcca acatctcagc
      301 cgtgccatga gtcgttttcg tgttcaaatt atgaatcagt ttgaacgaaa atcccatgaa
      361 tacaaggcta tcaagcgtta ctggaaactc atccaacagg atagtcgtaa actcagcgat
      421 aaacgttttt atcgccctac ttttcgcatg cacttaacaa ataaagaaat tcttgacaag
      481 attttaagct attcagaaga cttgaaacac cactatcaga tctatcaact cttacttttt
      541 cactttcaga acaaagaccc tgagaaattt ttcggactca ttgaggacaa tctgaagcag
      601 gttcatccta tttttcagac tgtctttaaa acctttctaa agaacaaaga aaaaatcgtc
      661 aacgctcttc aattacctta ttccaacgca aaaattggaa gcgaccaata atctcatcaa
      721 acttatcaaa cgaaacgcct ttggatttcg gaactttgaa aacttcaaaa aaaggatttt
      781 tatcgctctg aacatcaaaa aagaaaggac gaattttgtc ctttatcgag cttagctgac
      841 ttcaacccac tacagttgac aaagagcccc tttctcgagc ttagcttttc ttcaacccac
      901 tacagttgac aaagagccag aaaaatccct tgtttagtaa acaaaggatt ttgtgaattt
      961 tcaatttgat taaagttcga tgtcaccaaa caaatcagcc attgaaaatc ctgtttgtgt
     1021 ttctggaagt tcgaaatcac gcttttcttg acgtcttgga cgacgtggac gagcagcacg
     1081 tttttcttct ttttgtcctt cttcttgggc tggacgttct tcaagagctt taatagaaag
     1141 tgacacgcgt tctgcatctg cgttaacttc aagaacttta acttgaactt cttgaccaac
     1201 tttaagagct tcttttggat tttcaatccg tttgtgtgaa atttgtgata cgtgaacaag
     1261 tccatcgata cctggcaata cttcaacaaa tgcaccgaag tcagtcaaac gtttaactgt
     1321 tccttctact acatcacctt tagccaattt ttgctcaacg ccatcccatg gtcctggtac
     1381 tgttgcttta agtgaaagtg atacacgtcc ttcttcttcg ttaagatcaa ggattttcac
     1441 ttcaatttct tcaccaacag ttacaactga ttttggtgat acgttacgtt catgtgacaa
     1501 ttcagtcaag tgaaccaatc cgtcaacacc accaaggtcg acgaaagcgc cgaagcttgt
     1561 gatacgagca actttaccag ttacaacatc accaacagcc aatttaccga atacttcagc
     1621 gcgagctgct gcagtagctg cttcaacaac ttcacgacgt gaaaggatga agcggttttc
     1681 tttagcgtta acttctttga ttttagtatc aaattcttga cctacaaaac gctcagcatt
     1741 acgtacgaaa cgagtatcca acattgaagc tgggataaat ccacgaacac cttcaaattc
     1801 tactgaaagt ccacctttaa cggcacgcgt tcctttaaca gtaacaactt cttcttcgcg
     1861 accaacaagt ttgtcccatg ctttgcgagc ttcaaggcgt tttttagata caaggtatgt
     1921 aactgtatca gtatctttac caactacttg acgaagtaca agaacatcca atacttctcc
     1981 tactttaaca aagtcattga tatctgcatc acgatcgttt gtcaattcgc gaagagtcaa
     2041 gacaccttca acaccagttc cagagattgc aacgttagct tgagtcgcat caactgtcaa
     2101 tacttcagca ctaacaacat caccagtctc aacttgacta acgctattta gcaaatcttc
     2161 aaattcattc atctaaaaaa tcctccaaca atcaagtttt tcttaaactt gacatactta
     2221 taattttttt cctaagcacc cgcaaggaca tgttcatatc attcacgtct ttcaattata
     2281 aaaataaaat accctaagat attttgcttt ttatcttgat tattacctaa gaactggggt
     2341 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
     2401 ccccattaat gaaatggaga gagagggatt cgaacccccg aacccgaagg agcggattta
     2461 cagtccgccg cgtttagcct cttcgctatc tctcctacaa tcaacatgga ctattatatc
     2521 atgaaaattt caaaaaaaca agacttattt tgctaaatta taattttcaa tcaaaatttc
     2581 cacagctttt tccaattttt tataaaaact atcgacattc ccattcttta taatggcaaa
     2641 ttgtccgtct aattcggcaa tttctccgat aactttttta ccgatagtca atgtataacc
     2701 ttcaaaactg tctttaccaa cattaacttt ggcatccgct acttgaattt cgattttttt
     2761 atctttcttg ctcatttcat acctctcttc actttttcta ttttacaaga aaattctaaa
     2821 actagcaaga aaaaactcta tatcaggctt ctctgatata gagttttatg taaacaacct
     2881 caattgattt agcatagtta atagttagta aacaagcata attgactatc ctttttaggt
     2941 tatcagtgag tctcaccgcc tactaactgc aaatccttat ctttgtagtc tacaatagct
     3001 tcaatagcta cctctatgcc tcttacaata tcacatagac tcattcctgc agtattcggc
     3061 ttatttacta cttgttccat catatatgga atatgcatga aacctgctct catattaggg
     3121 aattttttat ccactaaata aagagcttgg tacatcaaat gattgcaaac aaaggttcct
     3181 gcactattgg atacaactgc cggaagtccc tgttttttga tagcttgtac catcgctttg
     3241 ataggtaaac tactaaaata ggccgatgct ccatcaatac gaatcggtgt atcaattggt
     3301 tgattgcctt cgttatcagg tatgcgagca tcatcttgat taatagccac tcgttcaggt
     3361 gttaagccgg tcctgccgcc tgcttgtcca atacaaagta cagcatctgg ttgatatcgt
     3421 aatatttctg cctctaaaac ttctgacgac ttataaaaaa ccgttggaat ttctacccag
     3481 cgaacttcag ccccattaat ctcagatggt aataatttta cagcctccaa agctggatta
     3541 atcttttcac ctccaaaagg attaaaacct gtaaccaata ttttcatttt attttccttt
     3601 actaaaatgc gagaaagtac attaagaata tgtgaataac aatcattact agagcaacac
     3661 ctgcttgagc ctttataacg ccattctgat ctttcatatc catcaatgct gctggtagag
     3721 cgttaaaatt agcagccatt ggggtcaata aggtcccaca ataacctgct gtcatggcaa
     3781 gagcaccagc cacaattgga ttagctccca gagcaaatac aaagggaact ccaacacctg
     3841 ctgtaataac ggtgaatgct gcaaaagcat ttcccataat cattgtgaat agaaccattc
     3901 caagaacata ggccaaaact cctataaagc gactatctga aggaacaata ccgctaatca
     3961 gatgagagat aacatcacca acacctgcca cagtaaaaat agcccccaaa gcccctaata
     4021 attgaggaac aatcccactt gttgaaactt gctgagtcat tcgattattt tctgataaca
     4081 gactcttagg gtgactattg gtaatcacaa gaacagaaat tgtagcaaac aaggcggcaa
     4141 ggctaatcga aatcttgcta aattctggaa tcatttgcgc taagaccaac gcaagtattg
     4201 ccatcagcat aactggaata aaaattttat ttttcaacct gttagattca atattggctt
     4261 tcatttcatc taaggatggc aaggttccga tacggacttg cttaaacaat gttaacagcg
     4321 ataataggat tacaataata ccaatactca tatttggcat ataggaacca cctataaacg
     4381 taatagacaa tagagtccaa aatgcagatg tcccaagtcg aactgggttt gttttatctt
     4441 tataactaca ataggctgta tggagaaatt gacaaccaat cacaatatag gtcaactcta
     4501 atagttgctt tgccaactct gtcatttttg ttctcctccc ctagtctttt ttgatatcaa
     4561 ttttttatca aataaataat tgtaaatccc cactacaata agtgttataa cagcaacaat
     4621 aatagatgta gaagcaatcc ctgcataatt gctttcatag cctaactgat ctaatgttcc
     4681 ccctatcaag aggactcccc cagcacctac aaacgtattt tgagcaaaga aatttccaaa
     4741 attttcattc gctgccgcac gcgcttttat tgtctcatct tcaacctctg ttaactttct
     4801 acctaattga gactctgcag ctgcttctcc cataggttga accaaaggtc tgacaaactg
     4861 agggtgtcct cctagacgaa ttgaaaagaa accagctaac tctcgaataa agaaataaac
     4921 tgtatagaag tttccaactg tcagaccttt aatctttcga atcaaatcga ttgatcgttg
     4981 cttgagtcca aaggtttctg acagccccac aagaggcaag gtaaccataa aaatcgtgag
     5041 cactcgctga ttgctaaatt cttttcccaa aatctccaaa aattcaacga gagaaacacc
     5101 tgaaactaaa gctgtaacca aaccagctaa gactactgtt gcaattgtat caaattttaa
     5161 aataaaaccc acaacaatga ttgctattcc tattaatcta atccactcca tatcaaactc
     5221 ctttatattc aaaatgacag tattttaaaa attttatcaa gatcaatacc attccttatt
     5281 taatgtattt ttctagttct ttttggtatt tgccattttt taaaaacctc gttatattct
     5341 tttgttgtaa caatatcttt ttgcaatttc attcctttaa agatatatgg atccccctta
     5401 ataccaactt gtgagtatgg ttttgagaat ggtactacgt tacttacaac tggagaacca
     5461 ccagatgaag ctgttggcat caataatgaa ctatctgtcg accaagcttg agctttggca
     5521 tatttttcat atctcttctc taggtcagtg gtctcagaaa cagcatcttc taacaatttc
     5581 ttatatttat ccaaaccagg tttagctaca acatccttat cttttccttt cgtaatacca
     5641 aggtgtttca tggcagaacc agattttgga tccataatat tcaagtaagg cgctggatct
     5701 tgatagcttg gagcccatcc tgtactattc aaatcatagt ctttttgaga aggagcaaca
     5761 ttgccgtatt tatcattttc catcaaacca tcaataacgt ctgtcctcga tgttcgagtc
     5821 gctatactgt agcccaatga tgctggatct actgcataga cataagaaaa tgttgtcggt
     5881 gcatctgctt ctttatcagt ttttccacaa gccactaaaa tagctgacgt gctcaggacc
     5941 actcctgctg ttaagagcca ctttttctat ttcataaaaa atctcctttg gtttatttta
     6001 atctactttt acaatccaac cttctggcgc ttcaatatcg ccaaactgaa tacccgtcaa
     6061 ttcattatat aatttacgcg tcacaggacc tacttctgtt tcactataga atacatggaa
     6121 atcatcacca tgttgaatac ctccaattgg agaaataacc gctgctgtac cacaggcacc
     6181 tgcctctaca aaacggtcaa gattatcaat tggaacatca ccctcaatag gagttaatcc
     6241 caagcgatgt tctgccaaat aaagcaagga atacttggta atagatggca agatagatgg
     6301 actcaatggt gttacaaatt cattatcagc tgtaattcca aagaaattag ctgatccgac
     6361 ttcttcaatc tttgtatgag ttgatgggtc cagatagata acatctgaga aatggcgtga
     6421 cttggccatt tttcctggta agagacttgc agcatagttt ccaccaacct tagccgcacc
     6481 tgtaccattt ggtgctgcac ggtcgtactc atcctgaatc aagaagttgg ttgggaccaa
     6541 accaccttta aagtaatttc caactggcat agcaaagatg gtgaaaatgt actcttctgc
     6601 cggttttacc ccgataatat ctccgacacc aatcaaaaga gggcgaagat ataaggttcc
     6661 acctattccg tatggtggta cgtattcttc attcgcacgg acaactgctt tacaagcttc
     6721 tacaaacatg tctgtcggaa cttgtggcat caagagacgg tcacatgtac gttgcaggcg
     6781 tttagcattt tcatcaggac ggaacagttg aacactgcca tccttagtac gataagcttt
     6841 caaaccttca aatgcttgtt gtccatagtg aagacttgga gaagactctg aaatatgcaa
     6901 agttgcatcc tctgtaagct ctccttgatc ccattgtcca tttttgaaat gagcaagata
     6961 gcgataaggt aatttcatat aggaaaaacc gaggttttcc caatcaatcg ttactgtcat
     7021 attccactcc ttattctaaa aacctatttc ttatattcta cactattttt ctaaaatagc
     7081 aagtatattt tgtaattttc agaaaattcc tccaataaaa accatcttag aactgattct
     7141 tcatttcact tatttatctt cagtaactac ttcctgaaga taagcgtcaa aaacttcttc
     7201 atctgaaatc gtgtcagaaa tgaagcttcc attgctagtg cgttctgaca agttcaagtc
     7261 ttgcaatcgg ctttcataga ttgttccttt attggattgg acaagcagag tttggtcgtt
     7321 cacatccact tccgtactga agaaatcgcc aacaaatcct tgctctgcaa ctgctcctgc
     7381 caagaagaca cgatgcggtt tgtttttcaa ctcacgcaag acttgtaatc ctcgtttggc
     7441 acggctggtt gctagaattt cctcaatgga aacacgtttc aagcttccac gctgggtcaa
     7501 gaggtagaag gacgaagtat tacagataaa gccagattgg aggacatcat cttctttcaa
     7561 attcatagcc ttgacacctg ctgccttagc accgacaacc ggaacctctt cgatattgaa
     7621 acgcagggca taaccatttt gactaaccaa gacaacatca tctagtttaa tcggagccac
     7681 tgctacaatc tgatctgtat cgtctttgag cttagcatac ttgacagact tagatctata
     7741 ggtccgccat ggagtgaatt cttttcgctc tacccgtttg atttgaccaa ggcgagtcgc
     7801 tgcaaagtag gttgtcgcat cgtcaaactg atccagtact tccacataaa ggatttcttc
     7861 attcgtttca aagtttgtga tggtttggct cagatgctct ccgatgtcct tccaacgaat
     7921 atctgccaac tcatggattg gtctgtagat gacatttcca agacttgtga acatcaagag
     7981 gtgctgggtt gtcttggcag attgaacaaa aatcaaacgg tcatcatcac gcttgccaat
     8041 ttcttccaag gtggaagccg caaaggaacg tggactggta cgcttgatgt aacctgcctt
     8101 ggtcacgctg acgtaggtat cttcctcagc gataagacta gctgtatcaa tctcaattgc
     8161 tttcgcagtg tcttctaaag aactcaaacg aggagttgca aagttcttct tgacctcacg
     8221 aagttctttc ttcatgagat tgtacatagt cctttcatca ccaataatag ccgccagcat
     8281 agcaatcttc tcacgaagct ctgcttcttc ttcctgcaag acaaccacat cggtattggt
     8341 caaacggtac agttgcaaag ttacgatagc ctcagcctgt tcttccgtaa aatcatagct
     8401 aactttgagg ttttccttgg cgtccgcctt attctcagaa gcacggataa gagcaatgac
     8461 ttcatccaaa atcgaaatca cacgaatcaa accttcgacg atatggagac gtttctcagc
     8521 cttttctttg tcaaagcgtg aacgcgccaa aatcacttct cgacggtgag cgatgtagct
     8581 agacaggatt ggaacaatcc caacctgacg aggtgtgaaa ttgtcaatcg ccaccatatt
     8641 aaagttgtag ttgatttgta ggtcggtgta cttaaataag tagttgagaa caagctcagt
     8701 attagcgtct ttcttaagtt cgatagcgat acgaagacca tcacggtcag actcatcacg
     8761 aacctcagca attccagcta ccttgttatt aacacgaaca tcatcgattt tcttgactag
     8821 attggcctta ttgatttcat aaggaatctc agtaataacg atttgttcct taccaccttt
     8881 tagcttttca atttcagtct tggaacgaac aaccacgcgc cctttcccag tctcataagc
     8941 tttcttgatt tcatcacgac cctgaataat agcccctgta gggaagtctg gtccaggcaa
     9001 gaattccatg agtttatcaa tctttgcagt tgggtggtca atcatgtaaa ctgcaacatc
     9061 tatgacctca gctaaattat ggggaggaat gtctgtggca taaccagccg aaatcccagt
     9121 cgaaccattg accaagaggt ttggaaaggc tgctggcaag accgttggtt ctttctccgt
     9181 atcgtcaaag ttccatgcaa aaggaactgt ctttttctcg atatcctgaa gaaggtagcc
     9241 tgcaatttca gacaaacgtg cctcagtata acgcatagcc gcaggaggat ctccgtccat
     9301 agaaccgtta ttaccgtgca tttcaactag aatctcacga tttttccagt tctgtgacat
     9361 acgaaccatg gcatcataga tagaagaatc cccgtgtggg tggaaattcc ccatgatgtt
     9421 cccgactgac ttggccgact tacggtagct cttgtcaaaa gtattgctat ccttattcat
     9481 agaataaaga atacggcgct gaaccggctt caacccatca cgaatatctg gcaaagcccg
     9541 gtcttgaata atgtacttgg agtagcgacc aaagcgctct cccatgatgt cctccaggga
     9601 catgttttga atgttagaca taagatacaa agcccataaa ataccaagtg aaaatagaaa
     9661 attcttgaag taagcaaact cacaagagaa tttatctttt tcacacaata tctagggcgt
     9721 gttcaactcc tttcaaagaa tgtagagtag gtttttatgc agtaaaagat attttacggg
     9781 aattcgtccc gtgttcagtt acgataagta accaaactat cctgtttgta tttttcaata
     9841 tgaaaatctg gttttccaaa attagtctta gtttgtgtct tagccgctcc cttaaacgcc
     9901 tctttgagat aagcactcat agaagattct tcattaataa tcctgcaatt ttttcaaacc
     9961 aagattttca aactgctttt tcacatagtc attcacatcc gactctaatt tccagtttac
    10021 taacatatta ttttctttca ttaaaacact gtcgcttctt ctagcgtaaa cttgacatta
    10081 tcttcaatcc atttacggcg tggttctacc ttatctccca tgagaacatt gacgcggcgt
    10141 tcggcgcgcg ctaaatcttc aattgtgaca cggatgaggg tacgtgtttc tgggttcatg
    10201 gttgtttccc agagctggtc cgcattcatc tcaccaagtc ctttgtatcg ttggagggta
    10261 gcgcctttac cgaactgttt acggagttct tctagttctc cgtccgtcca agcgtaggcc
    10321 acttcttctt tcttgccttt acctttggac atcttgtaaa gaggtgggag ggcaatatag
    10381 acatgacctg cctcgactag cggacgcatg taacggtaga aaaatgtcaa gagcaaggtc
    10441 tggatatggg caccgtcggt atccgcatcg gtcatgataa tgatcttatc atagttggca
    10501 tcttcaatag agaagtctgc tccaacaccc gcaccaatgg tataaatcat ggtattgatc
    10561 tcttcatttt tgaggatatc cgccatcttg gccttggctg tattgataac cttaccacga
    10621 agaggtagaa tagcctggaa cttgcggtca cgaccttgtt tggcagaacc accggcagag
    10681 tccccctcaa ctagatagag ttcattctta gcaggattct tagattgggc tggggtcaat
    10741 ttcccagaca acaagccctt atctttcttg tttttcttcc catttcggct ctcatcacgc
    10801 gccttacgtg ctgcttcacg agcatcacgg gccttgatag ccttgcggat gaggttagaa
    10861 gctaattccc cattttccat aaggaaaaag gtcaacttat cagccactat tccatccaca
    10921 actgggcgag ctagggggct tcctagttta tccttggtct gtccttcaaa ctgcaagtgt
    10981 tcttcaggaa ctaagataga aagaacggcc gctagtccct cacgatagtc tgaaccttca
    11041 aggtttttat ctttttcctt gagaagacct gttttacgtg catagtcatt catgaccttg
    11101 gtaatggcag acttgagtcc tgtctcgtgc gttccaccgt ccttggtgcg aacgttattg
    11161 acaaaggata gaatgttatc tgagaatccg tcattgtact ggagggctac ttccacttga
    11221 aaaccattgt cttccccttc aaagtaaaga actggcgtca agatttcctt atcttcgttg
    11281 agataagaaa caaaatcttg tactccattc tcatagtgga actcaatcgc ttcatctgtt
    11341 cgcttgtccg ttaaagacaa ggtcacattt ttcaagagaa aggctgattc attaaggcgc
    11401 tctgaaatgg tattgtactt gaaatctgtc gtagaaaaga tagtcgcgtc aggcataaaa
    11461 gtaactttgg tgcctgtttt agacttgggt gctgtaccga ttttcttcaa agtcgtgaca
    11521 ggttttccac cattttcgaa acgttgcttg taaactgcgc catcacgggt aatttcaact
    11581 tctaaccagc tagaaagggc gttaacaacg gaagaaccca ctccgtgaag tccacctgat
    11641 gtcttatagc caccttgacc gaatttccct ccggcatgaa gaatggtaaa gataacctca
    11701 acagttggaa ttcccatagc gtgcatacct gtcggcatcc cacgtccatg gtcttgaacc
    11761 gttagactac cgtctttatt gatagttaca tcgatacgat caccaaaccc agacaaggct
    11821 tcatcgactg cattatcaac gatttcccaa actaggtgat gaagaccagc gccatcggtc
    11881 gatccaatat acatccccgg acgttttcgg accgcatcca acccttctag cacctgaata
    11941 gcatcatcat tataattgtt aatattgatt tccttttttg acacaaggaa cctcctattc
    12001 gttcatcttt actattctac aggttttcca aggattttgc aaaatttttc tttctccgat
    12061 gtgacaattt tagcagagat tctctgcttt tctttcccaa ttcatgatat aataggagta
    12121 tgattacaat agttttatta atcctagcct atctgctggg ttcgattcca tctggtctct
    12181 ggattggaca agtattcttt caaatcaatc tacgcgagca tggttctggt aacactggaa
    12241 cgaccaatac cttccgcatt ttaggtaaga aagctggtat ggcaaccttt gtgattgact
    12301 ttttcaaagg aaccctagca acgctgcttc cgattatttt tcatctacaa ggcgtttctc
    12361 ctctcatctt tggacttttg gctgttatcg gccatacctt ccctatcttt gcaggattta
    12421 aaggtggtaa ggctgtcgca accagtgctg gagtgatttt cggatttgcg cctatcttct
    12481 gtctctacct tgcgattatc ttctttggag ctctctatct tggcagtatg atttcactgt
    12541 ctagtgtcac agcatcgatc gcggctgtta tcggggttct gctctttcca ctttttggtt
    12601 ttatcctgag taactatgac tctctcttca tcgctattat cttagcactt gctagtttga
    12661 ttatcattcg tcataaggac aatatagctc gtatcaaaaa taaaactgaa aatttggtcc
    12721 cttggggatt gaacctaacc catcaagatc ctaaaaaata aaatgccagt tctgtgaact
    12781 gccccccaaa agttagacaa ataatttatc caaaggattt agttctgtac tgcacaggac
    12841 taagtccttt tagttttacc ttaattcgtt tattgttgta gtaatcaaca tagtctacaa
    12901 tagcttgttc caattgctta agcgactgaa acgacttctc ataaccgtaa aacatttccg
    12961 atttcagaat cccaaagaag gactccatca taccattgtc tgggctgtta cccttacgtg
    13021 acatagatgc ttgaattccc ttactctcta ggaaccgatg ataagaatcg tgttgatatt
    13081 gccagccttg gtcactatgg agaatcgtat tctcatagtg cttctctgtg aatacctgtt
    13141 ccaacattgt ttgtacttgt tctaagttgg gtgaagttga aagattatag gcgataattt
    13201 cgctattaaa gccatctaaa actggtgata agtaaagctt ttgagtactt gctggaatgg
    13261 caaattctgt cacatctgtg tagcactttt ccattgtttt agagccttca aattggcctt
    13321 gaatgagatt ctctgctttc ttaccaacgt ctcctttatg agaagaatat tttcgtttct
    13381 gtcgcatttt agcttgtaaa ttgagtactt ttatcaagcc ttgaactctt ttatgattta
    13441 ccagataagc acgatttctt agttctaaat gaacccggcg ataagcataa tttcctttgt
    13501 gttcgataaa gatggattga atttcagctt taagctcttg gtccttatct gttttgtcta
    13561 gctgtttcaa gtgatagtag taggtccaac gagctagttt aatggatttt agaagaagaa
    13621 ctaacgaaaa ctcagtcatt aattcttgaa caatttctgt ccttcttctt tctctttttc
    13681 ctccttcaat cggagttctc ttaacttttt taggatggca ttctccgctc tcaggtactc
    13741 attttctgct tgaagatatt ctaattctgt cctctcttca ggtctcgttt ttggcttacg
    13801 tcccatttta ggtactctcc ctcttgtttt ctcaacaata gtatacccgt ttttcctgta
    13861 ttgtgctagc cagttaagaa gtatcgtacg acttgggagg ccgtattcaa gagaaactct
    13921 atctttagtc cagccttcat gtcagacttt atgaatcatt tcttgtttta aatcaggaga
    13981 atagtaacga ttttt
//
LOCUS       DAVDWM010000050        13870 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVDWM010000050 DAVDWM010000000
VERSION     DAVDWM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.050"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            226..1665
                     /locus_tag="VUW49_001771"
     CDS             226..1665
                     /locus_tag="VUW49_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7981652.1"
                     /translation="MSREKKRGTAVHLKSYINSVLLILYTGIASLFLFQIFRYNILNF
                     RNLNLLVALLVVLVFILGVSLIVRKKAEKLTMLLLILAVASSSISLLAVQQFIGFTSR
                     LNATSNVSEYAISLVVLKDSEISELEQVSHVMAPTDTDYEAIQSLLADIKEKQGKDLI
                     VDKSKSYLEAYKSLISGDTKVIFLNSIFEHMIESDFPDFRDKIKKIYTKEMTKKVEAP
                     KVSKGQTFTIYFSGIDTYGPINSVSRSDVNILMTVNSETKKILLTTTPRDSYVAIADG
                     GKNQKDKLTHAGIYGIDSSIHTLEHLYGVEINYYVRVNFTSFLKIVDLLNGIDVDNSQ
                     EFTIDGNDYPVGNLHLNSKSALGFVRERHSLADGDRDRGRNQQKVLAAIIQKLTSIES
                     LKNYRQIMQGLEDSIQTNLSPTTIMDLANTQLESGGNYKINSQDLKGTGRMDLPSYAM
                     PDSQLYMMEIDENSLSAIKENIQAVLEGR"
     gene            1667..2398
                     /locus_tag="VUW49_001772"
     CDS             1667..2398
                     /locus_tag="VUW49_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HET7981653.1"
                     /translation="MIDVHSHIIFDVDDGPKSIEDSRALLLEAYDQGIRTIVSTSHRR
                     QGMFETPEDKIAENFRAVQKIARDIADDLTILYGAEIYYTQDIINKLEKKTFPTLNGS
                     RYALIEFSSETSYRSIHSALQQLLLSGITPLIAHIERYDALENNKQAVLELIRMGCYT
                     QINSSHLLKKKLFKDDAKIMKKRVHYFLKHDLVHIVASDMHNLDTRPPYMAEAYEIIS
                     KRYGKEKAEEFFIKNPQRILMDQLI"
     gene            2417..3004
                     /locus_tag="VUW49_001773"
     CDS             2417..3004
                     /locus_tag="VUW49_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7981654.1"
                     /translation="MYKVIKRFIDIALALLGIVLLSPLFLGIIVFIKLESKGPIFFKQ
                     KRIGLHKKEFYILKFRTMRIDTPSAVPTHLLKNPYQWITKVGKVLRRTSLDELPQIWN
                     ILVGHMSLVGPRPALWNQFDLIEERDKYGANDILPGLTGWAQINGRDELSIPVKAKFD
                     GYYVKNCSFVLDCRCVVESFLVVFKRYGHREGGAD"
     gene            3017..4147
                     /locus_tag="VUW49_001774"
     CDS             3017..4147
                     /locus_tag="VUW49_001774"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7981655.1"
                     /translation="MGNRKKVFVVANVVKEHILKFHIPTIKEMTKRGWTVDVGASGDE
                     TVPYCHQQYSLPHQRSPFHLGTLKSIWKLRKILKSGGYDIVYCHTPVGGLVGRLASLG
                     LRKQGTRVIYFVHGYHFYKGATFFNWLVYYPIEKLLSLFTDSIILINQEDYHLTKTKF
                     KQVQSYLVSGMGVKQERFSPQSNQVRERYRKELKLPEDATVLIYCAELIKNKNQTFLL
                     HAMKKLVDQGENLYCLLVGIDYTKGEMVEYIQSLNLSDRVLLLGWREDIANLYACSDI
                     CVATSIREGFGLNIVEAMFCHVPVVATINRGHASIIQDGQNGLLVQLGNTKQLVEAIY
                     VLMKDTKKKQELVEQASANLEQYHSQKIVNSLLEIIEQTAMK"
     gene            4165..5574
                     /locus_tag="VUW49_001775"
     CDS             4165..5574
                     /locus_tag="VUW49_001775"
                     /inference="COORDINATES: protein motif:HMM:NF025654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET7981656.1"
                     /translation="MKIQIDKIYYLFFWLVVSFGYFFMMPTFTEDLEGVVSASLVQLL
                     LFIIGYVYVSYLNRSFISLYSIFMIVFYLFQNGQVLLYSLGVEYDYFYVLRYDETIVL
                     QSVIFSTQCLIAAFMAGVFSTKKEVSKPLYSYMDQLEREKLITTGKLFWGAFAIFALP
                     FMMMKLVITSTSGYFAMIRFVGSLPTITVLFEKMFIASSVFLIVYLKSEETWSKFLKV
                     VILGWSIMAALTGDRTVGLAGIVTLALIQTLIGNRKKKIKFSQYALLVGAAVVVMYLM
                     SFAFQFRMQQDSKVSGLQTAVVEMIGTLGFSFFPLVLTIRIVPTSINFFRGKSYIAAI
                     ITGLIPSNFDFLHLTNTLSEWNAYPTELLDTIYHYGFGLDYSLIAEAYINFGSYGWIA
                     IFFLCSLIAYFVRDVDFKRKDNLFSQYASLILLYSWFTLPRRKSYFIFNNFFWYVLFF
                     GLALILVSRSIKQRKEFKK"
     gene            5571..6338
                     /locus_tag="VUW49_001776"
     CDS             5571..6338
                     /locus_tag="VUW49_001776"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7981657.1"
                     /translation="MKISIIMGIHNGEKRFETAVQSIFNQTYDNWEFIICDDASTDKT
                     FEKLQGLYGQDSRFILLKNEKNVGLSATLNRCIEASSGEFIARMDDDDICYPERFERQ
                     VDYLFHHPEIDFVSSSIDIFDGEVVVGTRILLDFPSKKDLIWNSPFVHPVTMFRRDAL
                     LEVGGYRVSPETVRGQDYDLFMRLYSCEKKGGNILEPLFRYTIDQNTMKRRTFKARIG
                     EMIIRYKGYRAMKVPFYNYVFIFKPLVAHLVTLVKRR"
     gene            6344..7519
                     /locus_tag="VUW49_001777"
     CDS             6344..7519
                     /locus_tag="VUW49_001777"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7981658.1"
                     /translation="MKILITTHTYLPNKDGVQFVNQYLAEGLVKRGHQVTVLTYCYPS
                     RTRVPREVINGVRVIRWNARTSCTFHKGEKLAYQNFILNSQSDYDVLVNVGTQTALTD
                     WLFPIFSQLTIPKVLYIHSIWDFNWLHWHRQSFKRFVAKAWANVRWKYYYWTKASIFK
                     QYHEVIQLHPKDYSVAFFKEKYGIESQILENAADASFFQNRNAVKKQSYIVNVSNFND
                     RKNQKKAVEYFLKSNLPNEWKLVLVGSEKNQYLQSLQTFEKELRNQLGITSGKEIEYR
                     IGLSREEVVKTVKEASFCLMTSIWEAFPISLIEVMAAGIPFISSDVGIVNYLSGGITA
                     CSDQEFIRAIEEFASCPEIRNQYGKKGQMEAKEHYQVDDKVKELEALLQKVVKEEKE"
     gene            7516..8481
                     /locus_tag="VUW49_001778"
     CDS             7516..8481
                     /locus_tag="VUW49_001778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981659.1"
                     /translation="MKKIVFLCPYFGTLPPHTQLWLNSCKMNPSVTWYLFTDDKRKFD
                     YPENVQVFYTTLEETKALYQKKFDFEISLEGAYKLGDYKPLFGYLYEEMIQEFDAWGH
                     IDVYDEIYGDIRAFVTDELLEKYDKLMIFGHMGIYKNSPEVNRRFELSSDLNRTYQEI
                     FSSSQFYNFEEYVAGSITRIYQKNGFPIGKLDEVIADLSGTSYHFRRGYISDDFETFT
                     YLPYEPMIFSWEEGKTYCYSVKNHQVQKDEFMYVHFKRRKMIQDIPDDASTYLIVPSG
                     FALMPEEITVDLIRKYSQKKLFYPVYFEEKWKGFKRLWNKIWRSE"
     gene            8478..9359
                     /locus_tag="VUW49_001779"
     CDS             8478..9359
                     /locus_tag="VUW49_001779"
                     /inference="COORDINATES: protein motif:HMM:NF025663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET7981660.1"
                     /translation="MKNILKKLIQQLPDKLFLQIKYFYIFKKKLNLKNPKTFNEKLQW
                     LKLYDRNPFYTILADKILVKDYIAKTLGEEYVIPTLGVYHHFDEIDFDELPNSFVMKT
                     NHDSGSIIIVKNKEDLNIQEAREKLQKSLERDYFIFGREWPYKNIERKIIIEEFMTDE
                     SNQELKDYKIYCFNGLPKFVHVDYDRFTNHKRNIYDSNWKQQEFMYAFPSDKNKTIEK
                     PAELSEMIELVRKLSKEMPFVRCDFYIVNHKILFGEMTFCPGSGFEKFYPEQYDVEFG
                     KDITLPEKHEWKQKKNV"
     gene            9335..10750
                     /locus_tag="VUW49_001780"
     CDS             9335..10750
                     /locus_tag="VUW49_001780"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014051.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET7981661.1"
                     /translation="METKEKRLVHNTIIFTIGSLGSKFIQFLLVPLYTYTLTASQFGI
                     TEILLTAVNLLIPVFSISIADGLLRFGLDKTLRRENVLKSAFIISILGTILSIISIPI
                     FSLYPTLSEWMVYFIIILNLRMYRDVFAIQLKVEGKNTLFACDSMIYTFVLSLASIVF
                     LVPFSLGISGYFFAYIVSNGISIFFILFFGGVWKSFTSGRFEKQLMIQLLKYSAPMIL
                     NGIAWWITNASDRFMLQWFMDDRAVGLYGVVAKLPLLIGTFTGVFNQAWIISAVEEFE
                     EENEEWFYQKVFHQYYAALFLSVSVFLLLLQPFMKVYVSPSFYEAWQYAPFLLLSSVV
                     SGIAAFMTGFYVAQKKNLNIIYTTIAGAFANILLNAMFIPMLGVLGASIATFLSWFVI
                     AIYRMKDVENFACFPLDKKVFWYLFLLCIQTITMTFLPILGIVFSVVLIPYFFYQEQE
                     FLAVLFDKGRKKVCSFKKSKR"
     gene            10723..11385
                     /locus_tag="VUW49_001781"
     CDS             10723..11385
                     /locus_tag="VUW49_001781"
                     /inference="COORDINATES: protein motif:HMM:NF020514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HET7981662.1"
                     /translation="MFIQKIKKIIRVFLRKSLVKDYRQRIQNKEFTLITSNCVGGLIS
                     HDLGLQFRSPTINMYIEAADFIQFCSHLERYLKEDLSFIEVNQDGHFVALCGDIKIYI
                     VHYNSFEEFAKKWSERAKRVDFDQLYLMMSERDGCRYEDIVKFDTLPYKNKVIFTSKK
                     MPEIQSAFYIPGIETKNNQKHKVKSVTTYLNSFTGKRYIDLFDFVEFLNTGRKQLSKY
                     EK"
     gene            11375..12457
                     /locus_tag="VUW49_001782"
     CDS             11375..12457
                     /locus_tag="VUW49_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7981663.1"
                     /translation="MKSKIILKNKTILVTGAAGFIGANLVMSLLQEENPLQIIGIDNL
                     NDYYDVSLKEYRLSTIQEECQSSLNKKWVFIKGDIADSDLMEEIFETYRPQIVVNLAA
                     QAGVRYSITNPDVYMKSNIIGFHTILEKCRKYQENIEHLVYASSSSVYGGNTKIPFSE
                     LDAVDNPVSLYAVTKKTNELEAHAYSKLYNIPTTGLRFFTVYGPAGRPDMAYFSFTKQ
                     LIDKKPIEIFNYGNCQRDFTYIDDIVEGVKRVMTGAPQKEIGEDGLPIPPYAIYNIGN
                     HQPENLLNFVEILQEKLIAASILPEDYDFSSAQKLVPMQAGDVVATYADISLLEKDFN
                     FKPKTSLRNGLRNFAEWYRNFYQRGE"
     gene            12459..13685
                     /locus_tag="VUW49_001783"
     CDS             12459..13685
                     /locus_tag="VUW49_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET7981664.1"
                     /translation="MKITVVGMGYVGLSLAVLLAQHHEVTALDIIDKKIQLINEKKSP
                     IQDEYIEQYFLEKNLHLSATTNEVEAYKDAEMIIIAAPTDYDTHKNFFDTSAVEQVIE
                     NIISMNNEALIVIKSTVPVGYTESIREKYGKKNILFSPEFLREANGLYDNLYPNRIIV
                     GTDLNDGALVSKAHEFVSLLQEGALKENIDTLIMGFSEAEAVKLFSNAYLALRVSYFN
                     ELDTYAELKGLNTKAIIDGVGLDPRIGQFYNNPSFGYGGYCLPKDSKQLLSNYHDVPE
                     NIISAIVSSNETRKKFIAERILEIVGANHIDEYYNLKQEIVVGIYRLVMKKGSDNFRH
                     SSIQGIIKRLKANGVTLMIYEPSLPDGQLFFGSEVVHDFPKFKEKSHLIVANRFDETL
                     MDVKDKVYTRDLFGRD"
BASE COUNT         4667 a         1873 c         2625 g         4705 t
ORIGIN      
        1 gcaggaagtc tattttggaa tgctattatg tttagttgtg ctcaatgaaa atgaaagaac
       61 tagtaagcta ggcgcagtca gctcaaaaca gtgttttgag gttgtagata gaactgacga
      121 agtcagtaac atatatatga caaaccgatg ctgacgtggt ttgaagagat ttttgaagag
      181 tataagacgg tattttaagg gaatgattac tatttgaggt attttatgag tagagagaaa
      241 aaaagaggaa cagcagtcca cttgaaatct tatatcaata gtgtgctgct aatcctgtac
      301 acaggaatag cgagcctatt tctttttcag atattccgat acaatatttt aaattttagg
      361 aatctcaatt tattagtagc tctgctagta gtattggtat ttattttagg agtgagtttg
      421 attgtccgaa aaaaagctga aaagttgaca atgcttctcc taatcttagc tgtcgcaagt
      481 agttcaatat cgcttttggc tgttcaacaa tttatcggtt ttacaagtcg cttaaatgca
      541 acttcaaacg tttcagaata tgctattagt cttgtagttt tgaaagatag cgaaattagt
      601 gaactagaac aagtatcaca tgtgatggcg cctaccgata cagattatga agctattcaa
      661 agtttgttag cagatataaa agaaaagcaa ggtaaagatc taatagtaga taaaagcaaa
      721 tcttatttag aagcctataa gagtttgatt tcaggtgaca ccaaggtgat ttttttaaat
      781 agtatttttg aacatatgat tgagtcggat tttcctgact ttagagacaa aattaagaaa
      841 atatatacaa aagaaatgac aaagaaagta gaagctccaa aggtttcaaa ggggcagact
      901 tttactattt atttcagtgg gattgatact tatggaccaa ttaactctgt atcacgttcg
      961 gatgtcaata ttttgatgac agtcaatagc gagaccaaaa aaattctttt gacaactaca
     1021 cctcgtgact cctatgtcgc aattgcagat ggaggaaaga atcaaaaaga taaattgact
     1081 catgctggaa tttatggtat tgattcttcc attcatacct tggaacattt atatggtgta
     1141 gagattaact actatgttcg tgtaaatttc acttctttct taaagatagt tgatttattg
     1201 aatggtattg atgtggacaa tagtcaagaa tttacaattg acgggaacga ctatcctgtt
     1261 ggaaatctcc atctaaattc taagtcagca cttggtttcg ttcgagaacg tcattctcta
     1321 gcagatggag atcgtgatcg aggacggaat caacagaagg tgctcgctgc aattattcaa
     1381 aaattaacct caatagaatc attgaaaaat tatcgtcaaa tcatgcaagg attagaggat
     1441 tcaatacaaa caaatctctc tccaacaact atcatggact tagccaatac tcaattggaa
     1501 agtggaggga attataaaat aaattctcaa gatttaaaag ggacagggcg gatggatctt
     1561 ccttcttatg caatgcctga tagtcagttg tatatgatgg aaattgatga gaatagcctc
     1621 tcagctatca aagaaaatat tcaggctgtt ctggagggta ggtaagatga tagatgttca
     1681 ttctcacatt atttttgatg tggatgatgg gccaaagtcc atagaagata gtagagcttt
     1741 gttgttagaa gcctatgatc aaggaattcg gacaattgtt tcaacatccc accgccgtca
     1801 aggtatgttt gaaacaccag aagacaagat tgcggaaaat tttcgtgcgg tacagaaaat
     1861 agctagagat attgcagatg atttaacaat tttatatggt gcagaaatct actatactca
     1921 agacattatc aataaacttg aaaagaaaac tttcccaact ttaaatggga gtcgatatgc
     1981 cttgattgaa tttagtagcg agacttctta tcgttctatt catagcgctc ttcagcagtt
     2041 gcttttgtca ggaattaccc cgcttatcgc ccatatcgaa cgttatgatg ctcttgaaaa
     2101 caataaacaa gcagttctcg aattgattcg tatgggctgt tatacacaga ttaatagttc
     2161 ccatctactc aaaaaaaaat tatttaaaga tgatgcaaaa atcatgaaga aacgagttca
     2221 ctattttttg aaacatgatt tagtccatat tgtagcaagt gacatgcata atcttgatac
     2281 tagaccgcct tatatggcgg aagcttatga aatcatttct aaaagatacg gaaaagaaaa
     2341 agctgaagaa ttttttataa aaaatccgca acgaatccta atggatcaat tgatttaatg
     2401 aaaaaggaaa caacaaatgt ataaagttat caaacgattt atagatatag ctttagcttt
     2461 gctaggaatt gtattattat caccactatt tttaggaatt atagtgttca taaaattaga
     2521 atctaaaggg cctattttct ttaaacaaaa aagaattggg ctacataaaa aagaatttta
     2581 cattctaaaa tttagaacga tgagaattga cactccttca gctgtcccta cacatctttt
     2641 aaaaaatcct tatcaatgga ttacaaaggt tggtaaagtc ttacgtagaa cctctctgga
     2701 tgaattgcca cagatttgga atattttggt cggtcacatg tcgcttgtag gaccaagacc
     2761 tgccctttgg aatcaatttg atttgattga agaaagagac aaatatggag caaatgatat
     2821 tttacctggt ttaacaggtt gggcacaaat caatggaaga gatgaattat ctattccagt
     2881 caaagccaaa tttgatggct actatgtgaa gaactgttca tttgttttag attgtcgatg
     2941 tgttgtagaa agttttttag tagtatttaa gagatatggg catcgagaag gtggtgcaga
     3001 ttagaggaac tgatatatgg gaaatcgtaa aaaagttttt gtagtagcca atgtagtcaa
     3061 agaacatatt ctaaaatttc acattccgac tatcaaagaa atgacaaaaa gagggtggac
     3121 ggtagacgta ggtgcttctg gggatgaaac agttccctat tgtcaccaac aatattcact
     3181 gccccaccaa cgttctccgt ttcatctagg cactctcaaa agtatctgga aattgagaaa
     3241 aattttgaag tctggaggct atgatattgt ttactgtcat actcctgttg gaggtttggt
     3301 gggacgactg gcttctttag gattgcgaaa acaaggaaca agagtcattt attttgttca
     3361 tggatatcat ttttacaaag gagcaacttt tttcaattgg ttagtgtatt acccaattga
     3421 aaagctactg tcccttttta cagatagtat cattctaatc aatcaagaag attatcatct
     3481 cacaaaaaca aaatttaaac aggttcaatc ctatctagtg agtggaatgg gagtaaaaca
     3541 agaacggttt tcaccccaat caaatcaagt tagagaacgt tatcgtaaag aattgaaact
     3601 gcctgaggat gcgactgttt taatttattg tgctgagcta ataaaaaata aaaatcaaac
     3661 atttttactt catgctatga aaaagcttgt ggatcaggga gaaaatcttt attgcttatt
     3721 agtaggaatt gattatacca agggtgaaat ggtagaatat attcaatcct tgaatttatc
     3781 cgatcgagtt ttacttttag ggtggagaga agatattgcc aatctttacg cttgttctga
     3841 tatatgtgtt gcaacaagta ttcgagaagg atttggatta aatatagtgg aagcaatgtt
     3901 ttgtcacgtt ccagttgttg cgaccatcaa tagaggacac gccagtatta tacaagatgg
     3961 acaaaacggt ctattagtcc aattaggaaa tacaaagcaa ttagtagagg ctatttatgt
     4021 actaatgaag gatactaaga agaagcaaga attagtggag caagctagtg cgaatttaga
     4081 acaatatcat agtcagaaaa ttgtaaatag cttactagaa ataatagaac agactgcaat
     4141 gaagtgagtg aaggatgtga aaaagtgaaa atacaaattg ataaaatata ctatctattt
     4201 ttttggctgg tagtttcctt tggatatttt tttatgatgc caacctttac agaagattta
     4261 gaaggcgttg tcagtgctag tttagtacaa ttgttattat ttattatagg atatgtatat
     4321 gtatcttatt taaatcgttc ttttatctca ctgtatagta tctttatgat tgtattttac
     4381 ctttttcaaa atggacaggt attgttatat tcacttggag tagagtatga ttacttttat
     4441 gttttaagat atgatgaaac catcgtacta caatcagtta ttttttcaac acaatgtctt
     4501 attgcagcct ttatggctgg agtcttttct acgaagaaag aggtatcaaa acccctttat
     4561 tcttatatgg atcaattaga acgagaaaaa ctgattacta ctggaaagtt attttggggt
     4621 gcgtttgcga tttttgcttt accctttatg atgatgaagc ttgttatcac cagtacatct
     4681 gggtattttg cgatgatacg tttcgttgga agcctaccta caataacagt gttatttgaa
     4741 aaaatgttca ttgcttcaag tgtttttttg attgtttatt taaaatctga ggaaacttgg
     4801 agcaagtttt taaaagttgt tatcttaggt tggagtataa tggctgcttt aacaggagat
     4861 agaaccgtag gtttagcagg gattgttacg ttagctttaa ttcaaacatt aattggtaat
     4921 cgtaaaaaaa agattaagtt ttctcagtat gccttgttag ttggggcagc tgttgtagtg
     4981 atgtatttaa tgtcctttgc ttttcaattt aggatgcaac aagattctaa agtttcagga
     5041 ctacaaactg cggtagtaga aatgatagga actctagggt ttagtttttt cccactcgta
     5101 ttaacgatta gaattgttcc aacttctatt aactttttta gaggaaaatc atatattgca
     5161 gccatcatta caggactaat tccatctaat tttgattttt tacatttaac aaatacatta
     5221 tcagaatgga atgcatatcc aacggaatta ttagatacaa tctatcatta tggtttcgga
     5281 ttagattatt ctttaattgc ggaagcctat attaattttg gttcttatgg atggatagcg
     5341 atttttttcc tttgttctct aatagcttat tttgtaagag atgtggattt taaaagaaag
     5401 gacaacttat tctcacaata tgctagcttg attttgctgt attcatggtt tacccttcca
     5461 agaagaaaat cttattttat ttttaataat tttttttggt atgtattatt ttttggacta
     5521 gcactcatac ttgtctctcg ttctattaaa caacgaaagg aatttaaaaa atgaaaattt
     5581 caattattat gggcattcat aatggtgaaa agcgctttga aacagctgtt caaagtattt
     5641 tcaatcaaac ttatgataat tgggaattta ttatttgtga tgatgcttcc acagataaaa
     5701 cgtttgaaaa gttacaagga ttatatggac aggattcacg ttttatctta ttaaaaaatg
     5761 aaaaaaatgt aggtctttcc gcgacattaa accgttgcat tgaagcaagt agtggagaat
     5821 ttattgctag aatggatgat gatgatattt gttatcccga gcgctttgaa agacaagtcg
     5881 actatttgtt ccatcatcca gagattgatt ttgtttcttc atctattgat atttttgatg
     5941 gagaagtagt tgtaggtaca cggattttac ttgattttcc tagtaaaaag gatttgattt
     6001 ggaatagccc ctttgttcat cctgttacca tgtttcgtag agatgccttg cttgaagttg
     6061 gcggttatag agtttctcca gaaactgttc gtgggcaaga ttatgatttg tttatgcgac
     6121 tatattcatg cgagaaaaaa ggtggaaata ttttagaacc actatttcgc tatacgattg
     6181 atcaaaatac gatgaagcga cgtacgttta aagctcgaat tggggaaatg attattcgct
     6241 ataaaggtta tcgtgcgatg aaagttccat tttataatta tgtttttata tttaaaccat
     6301 tagtagctca tctagtaaca ctcgttaaaa ggagataatg tttatgaaaa ttttaataac
     6361 cactcatact tatcttccta ataaagatgg tgttcaattt gttaatcaat atttagcaga
     6421 aggattggtt aaaagaggac atcaagtaac ggtgctgacc tattgttatc catcacgtac
     6481 aagggtacct agagaagtga taaatggtgt tagagtcatt cgttggaatg ctcgaacaag
     6541 ctgtactttc cataaaggag aaaaattagc ttatcaaaac ttcatcttaa actcccaatc
     6601 agattatgat gttttagtga atgttggaac acagactgct ttaacagatt ggttatttcc
     6661 gatattttct cagctaacaa ttccgaaagt cttatatatt cattcgatat gggatttcaa
     6721 ctggttgcat tggcatagac aatcattcaa gcgatttgtt gctaaagctt gggcaaatgt
     6781 acgatggaaa tattattatt ggacaaaggc ttctatcttt aagcaatatc atgaagtgat
     6841 tcagctgcac ccaaaagatt atagtgtggc tttttttaaa gaaaaatatg gcatagaaag
     6901 tcagatttta gaaaatgctg ctgatgcttc tttttttcaa aatagaaatg ctgtcaaaaa
     6961 gcaatcttac attgtaaatg tttctaattt taatgatcga aaaaatcaga agaaagcggt
     7021 tgagtatttt ttgaaaagca atcttccaaa tgaatggaag ctcgttttag ttggttcaga
     7081 aaagaatcag tatttacaaa gtctacaaac ttttgaaaaa gaactccgaa accaattagg
     7141 tatcacttca ggtaaagaaa tagaatatcg catcggactg tcaagagaag aagtcgtaaa
     7201 aactgtaaaa gaagcttctt tttgtttaat gacaagtata tgggaagctt ttccaatttc
     7261 cttaattgaa gtgatggctg caggaattcc atttatctct tctgatgtag gaattgtcaa
     7321 ctatttgtct ggaggaatca ctgcttgtag tgatcaagaa tttataagag ctattgaaga
     7381 gtttgccagt tgtccagaaa ttagaaatca atatggcaaa aagggacaaa tggaagcaaa
     7441 agaacattat caagtggatg ataaagtaaa agagcttgaa gctttgttac aaaaggtcgt
     7501 aaaggaagaa aaagagtgaa aaaaatagta tttttgtgtc catactttgg gactttacca
     7561 ccccatacgc agttatggct aaattcttgt aaaatgaatc cgtctgtgac atggtatctt
     7621 tttacagatg acaagagaaa atttgactat ccagaaaatg tgcaagtatt ttatacgact
     7681 ttggaagaaa caaaggcgtt gtatcaaaag aaatttgatt ttgagatttc tttagagggg
     7741 gcttataaac tcggagatta taaaccttta tttggttatc tttatgagga aatgatacaa
     7801 gagtttgatg cttggggaca tattgatgtt tatgatgaaa tctatggaga tattcgtgca
     7861 tttgtaacag atgaattact agaaaaatac gataaattga tgatttttgg gcatatgggg
     7921 atttataaaa attctcccga agtcaatcgt agatttgaat tgtcgtctga tttgaatcgt
     7981 acttatcaag aaatattttc ttcatcacaa ttttataatt ttgaagaata tgttgcagga
     8041 agtattactc gtatctatca gaaaaatgga ttccccattg gaaaattaga tgaggtgatt
     8101 gcggatcttt ctggtacaag ttatcatttt cgacgagggt acatttcaga tgattttgaa
     8161 acatttactt atttaccata tgagcctatg attttttcat gggaagaggg gaaaacgtat
     8221 tgttattcgg ttaagaatca tcaagtgcaa aaagatgagt ttatgtatgt tcactttaag
     8281 cgtcgaaaaa tgattcaaga tataccagat gatgcttcta cttatttaat cgtcccaagt
     8341 ggttttgcct tgatgcctga agagataacg gttgatttga ttcgaaaata tagtcagaaa
     8401 aaattatttt atccagttta ttttgaagaa aagtggaaag gtttcaaacg attatggaat
     8461 aaaatttgga ggtcggaatg aagaacattt taaaaaaatt gatacaacaa ttaccggata
     8521 aactgtttct acaaattaag tatttctaca tttttaagaa gaaattaaat ttaaaaaatc
     8581 ctaagacctt caatgaaaaa ttacaatggc taaaattata tgatagaaat cctttttata
     8641 ccattttagc ggataagatt ttagtgaagg actatattgc caagacactt ggggaagaat
     8701 atgttattcc gactcttggt gtttatcatc attttgatga gattgatttt gatgagcttc
     8761 caaattcttt tgtgatgaaa accaatcatg atagtggcag tattatcatt gtaaaaaaca
     8821 aagaagactt gaatattcaa gaagcaagag aaaagctaca aaaaagtttg gaaagagatt
     8881 attttatttt tggtcgtgaa tggccttata aaaatattga acgaaaaatt attattgaag
     8941 agtttatgac ggatgaatcg aatcaagaat taaaggatta taaaatttat tgttttaatg
     9001 gtcttcctaa gtttgttcat gtagattatg accgttttac aaatcataag agaaatattt
     9061 atgatagcaa ctggaagcag caagaattta tgtatgcttt tccgtccgat aaaaataaga
     9121 cgatagagaa gcctgctgaa ctgtctgaaa tgattgaact tgtgagaaaa ttatcaaaag
     9181 agatgccatt tgtgagatgt gatttttata tcgtcaatca caaaattttg tttggtgaga
     9241 tgactttttg tccaggttca gggtttgaaa aattttatcc agaacagtat gatgtagagt
     9301 ttggaaagga tattacttta ccggagaagc atgaatggaa acaaaagaaa aacgtttagt
     9361 tcataatacg attattttta caattggaag tttaggttca aaatttattc agtttttgtt
     9421 agttcctttg tatacctata ctttaacagc ttctcagttt gggattaccg aaattttatt
     9481 aaccgctgtt aatcttttaa tacctgtatt ttctatttcc attgctgatg gattattaag
     9541 atttggatta gataagacct taagaagaga aaatgtcttg aaatcagctt ttatcatttc
     9601 gatacttggg actattttat ccattatttc gattccaatt ttttcgctct atcctacttt
     9661 gtcagagtgg atggtttatt ttatcatcat tttgaacttg agaatgtatc gggatgtttt
     9721 tgcgattcaa ttgaaagttg aagggaaaaa tacattattt gcttgcgata gtatgattta
     9781 tacatttgtt ttatctttag cgagtattgt ctttctagtt cctttttctt taggcatttc
     9841 aggttatttt ttcgcttata ttgtcagtaa tggaatttcc attttcttta tcttattttt
     9901 cggaggagtc tggaaaagtt ttactagcgg aagatttgaa aaacaattga tgatacaact
     9961 attgaaatat tcagcaccca tgattttaaa tggtattgca tggtggatta caaatgcctc
    10021 agatcggttt atgttgcaat ggtttatgga tgatagagcg gtaggtttgt atggtgttgt
    10081 tgcaaagtta cccttattaa tcggaacgtt tactggagta tttaatcagg catggattat
    10141 ttcagcagta gaagagtttg aagaagaaaa tgaagaatgg ttttatcaaa aagtttttca
    10201 ccaatattat gcagcactct ttttaagtgt ttcagtattt ttgcttctac ttcaaccatt
    10261 tatgaaagta tatgttagtc caagctttta tgaagcatgg caatatgctc cgtttttatt
    10321 attgtcaagt gtagtttcag gaatcgccgc atttatgaca ggtttttatg ttgcgcaaaa
    10381 gaaaaatttg aatattatat acacgacgat tgctggtgct tttgcgaata ttttgttaaa
    10441 tgcgatgttt attcctatgc ttggagtatt aggcgcttcc attgcgacct ttctatcatg
    10501 gtttgttatt gcaatctatc gtatgaaaga tgttgaaaac tttgcttgtt ttccattaga
    10561 taaaaaagtt ttttggtatt tatttctatt atgtattcag accattacga tgacttttct
    10621 gccaatcctt ggcattgttt tttcagtcgt attgattcct tatttctttt accaagaaca
    10681 ggaatttcta gcagttttat ttgataaagg aaggaaaaaa gtatgttcat tcaaaaaatc
    10741 aaaaagataa taagagtatt tttaaggaaa tctttagtaa aagattatcg acaaagaatt
    10801 caaaataaag agtttactct tattacttct aattgtgtgg gtggactgat tagtcatgat
    10861 ttgggtctac aatttcgttc tcccactatc aatatgtata tagaagcagc agactttatt
    10921 cagttttgtt cacatttaga acgctattta aaagaagact tatcttttat tgaagtaaat
    10981 caagatggac attttgtagc tctttgtggg gatataaaaa tttatattgt tcattataat
    11041 agctttgaag agtttgctaa aaaatggtct gaacgtgcca aaagagtaga ttttgatcaa
    11101 ttatatctta tgatgtctga acgagatggc tgtcgttatg aagatattgt gaagtttgat
    11161 acgttaccct ataagaataa agtaattttt acaagtaaaa agatgccaga aattcaatcc
    11221 gctttttata taccaggtat tgaaacaaaa aataatcaaa aacataaggt gaaatcagta
    11281 acaacttatc ttaactcctt tacaggaaaa cgatatatag atttattcga ctttgtagag
    11341 tttttgaata caggaaggaa acaattaagt aagtatgaaa agtaagataa tattaaaaaa
    11401 taaaacaatt cttgttacag gagcagctgg ctttattggt gctaatcttg tcatgagttt
    11461 attacaagaa gaaaatccat tacagattat tggaatagat aatttgaatg attattatga
    11521 tgtttcttta aaagagtatc gtttatcaac aattcaagag gagtgtcaat catctttaaa
    11581 taaaaaatgg gtatttatca aaggagatat tgctgattct gatttgatgg aagaaatttt
    11641 tgagacgtat agacctcaaa ttgtagtgaa tttggcagca caagcaggtg ttcgttattc
    11701 tatcactaat cctgatgttt atatgaaaag taatatcatt ggatttcata ctattcttga
    11761 aaaatgtaga aagtatcaag aaaatattga acatttagtt tatgcatcat cctcttctgt
    11821 ttatggtgga aatacaaaaa ttcctttttc tgaattagat gcagtagaca atcctgtttc
    11881 tttatatgca gttacgaaga aaacaaatga attagaagca catgcgtatt caaaattgta
    11941 caatattcca acaacaggcc tgagattttt tacagtttat ggaccagctg gaagacctga
    12001 tatggcttat tttagtttta caaagcaatt aattgataaa aagccaattg aaattttcaa
    12061 ttatgggaat tgtcaacgtg attttactta tattgacgat attgttgaag gcgttaaaag
    12121 agtgatgact ggagctcctc aaaaagaaat tggagaagat gggttaccaa ttcctcctta
    12181 tgctatctat aatattggaa atcaccaacc agaaaattta ttgaattttg tcgaaatctt
    12241 gcaagaaaaa ttgatagctg cttctatttt accagaagat tatgatttct catctgctca
    12301 gaaacttgtt ccaatgcaag ctggtgatgt agttgcaacc tatgcagata taagtctatt
    12361 ggagaaggat tttaatttca aacctaagac tagtcttaga aatggcttga gaaattttgc
    12421 agaatggtat agaaattttt atcaaagggg tgaatagaat gaaaattact gtagtaggaa
    12481 tgggatatgt cggattgtcc ttggcggtat tattagcgca acatcatgaa gtaacagcgt
    12541 tagatattat tgataaaaaa attcaactca tcaatgagaa aaaatctcca attcaagatg
    12601 agtatattga acaatacttt ttagaaaaaa atcttcattt aagcgcaaca acaaacgaag
    12661 tagaggcgta taaagatgcg gaaatgatta ttatagctgc tcctacagat tatgatacac
    12721 acaaaaattt ctttgataca tctgccgtag aacaagtaat cgaaaatatt atttctatga
    12781 ataacgaggc tttaattgtc attaaatcaa ctgttcctgt tggctatacg gagtcgattc
    12841 gagaaaaata tggtaaaaag aatatactgt ttagcccaga atttcttcgt gaagcaaatg
    12901 gattgtatga taatctttat cctaatcgaa ttatcgtagg tacagatttg aatgatggag
    12961 cattagtttc taaagcacat gaatttgtgt ctttactaca agaaggtgct ctcaaagaga
    13021 atattgatac tttaatcatg ggattttctg aagcagaagc tgtgaaatta ttttccaatg
    13081 cttatctggc tttaagagtg tcttatttta atgagttgga tacttatgct gaactgaaag
    13141 gattgaatac gaaagccatt attgatggag taggattgga tcctagaatt ggtcagtttt
    13201 ataacaatcc atcctttggt tatggtggtt actgtttacc aaaggatagt aagcaactgt
    13261 tgtcaaatta tcatgatgtt cctgaaaata ttatttcagc aattgtttct agtaatgaaa
    13321 ctcgtaaaaa atttattgca gaacgtattc ttgaaatagt cggagctaat catattgatg
    13381 aatactataa tttgaagcag gaaatcgttg taggtattta tcgtttagtc atgaaaaaag
    13441 gttcggataa ttttagacat tcttctatcc aaggaattat aaagagacta aaagcgaatg
    13501 gagtgacatt aatgatctat gaaccttcac ttccagatgg acaattattt tttggaagtg
    13561 aagttgttca tgattttcca aaatttaaag aaaaaagcca ccttattgta gccaatcgtt
    13621 ttgatgaaac attaatggat gtaaaggata aagtatatac aagggatttg tttggaagag
    13681 attaagggag ataggttttg aacaagttct attctcaaaa aatcacaccg ttactatctt
    13741 cgaataaatt aaaagcaaag caagtgattg atagaaaatc ggctctttgt caactgtagt
    13801 gggttgaaga aaagctaagc tcgagaaagg acaaatttcg tcctttcttt tttgatgttc
    13861 aaagtgataa
//
LOCUS       DAVDWM010000051        13682 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVDWM010000051 DAVDWM010000000
VERSION     DAVDWM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.051"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..962)
                     /locus_tag="VUW49_001784"
     CDS             complement(<1..962)
                     /locus_tag="VUW49_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET7981665.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGAR"
     gene            1586..1768
                     /locus_tag="VUW49_001785"
     CDS             1586..1768
                     /locus_tag="VUW49_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET7981666.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1778..2128
                     /locus_tag="VUW49_001786"
     CDS             1778..2128
                     /locus_tag="VUW49_001786"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET7981667.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYGDSAWEHLRAVTGID
                     LLSILTDIANKKQG"
     gene            2136..3326
                     /locus_tag="VUW49_001787"
     CDS             2136..3326
                     /locus_tag="VUW49_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET7981668.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(3841..4833)
                     /locus_tag="VUW49_001788"
     CDS             complement(3841..4833)
                     /locus_tag="VUW49_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981669.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            4993..6747
                     /locus_tag="VUW49_001789"
     CDS             4993..6747
                     /locus_tag="VUW49_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981670.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6731..8451
                     /locus_tag="VUW49_001790"
                     /pseudo
     CDS             6731..8451
                     /locus_tag="VUW49_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            8462..9091
                     /locus_tag="VUW49_001791"
     CDS             8462..9091
                     /locus_tag="VUW49_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET7981671.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGSYCVGYFTPCLILWCYLN"
     gene            8992..9549
                     /locus_tag="VUW49_001792"
     CDS             8992..9549
                     /locus_tag="VUW49_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET7981672.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFIAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGISFKNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            9537..10901
                     /locus_tag="VUW49_001793"
     CDS             9537..10901
                     /locus_tag="VUW49_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981673.1"
                     /translation="MVEIKNLSLDYGEERILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRFAI
                     ASVMCKTSPFVFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
     gene            complement(11584..12125)
                     /locus_tag="VUW49_001794"
                     /pseudo
     CDS             complement(11584..12125)
                     /locus_tag="VUW49_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12201..12977)
                     /locus_tag="VUW49_001795"
                     /pseudo
     CDS             complement(12201..12977)
                     /locus_tag="VUW49_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12901..13095)
                     /locus_tag="VUW49_001796"
     CDS             complement(12901..13095)
                     /locus_tag="VUW49_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981674.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
     gene            complement(13265..13615)
                     /gene="tnpB"
                     /locus_tag="VUW49_001797"
                     /pseudo
     CDS             complement(13265..13615)
                     /gene="tnpB"
                     /locus_tag="VUW49_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         4810 a         2109 c         2361 g         4402 t
ORIGIN      
        1 cgagcaccaa acaactgctc attttcagat ggtataccgt agtagaaacc tgttgccaat
       61 tcacgttggg caaccttcca catctcgtcc actaagtctt gtttgatagc ttcaaacttt
      121 tcagaacttt caagataggc atccacagcc gccttgtagc agttggttac tgttgatacg
      181 tagtgaatag acttcatacg tccttcgatt tttagactgt ccacaccatt ttcaatcata
      241 tctggaatgt ggtcaatcat agacatgtca acggctgaca ttgaaaattc ttctggaatc
      301 tcaccctgca aactcttacg ttctttccca aatggcatat cgtaaaggtc gtatttccaa
      361 cggcatgact gagaacatcc accacggttg gcatcacgca tactcatgtg gtttgaaaga
      421 gtacaacgtc cagagtatga aatacacata gctccatgga caaaggcttc aatttcaaca
      481 tctgtacgtt tgcggatttc agctaattct tccattgaaa cctcacgcgc taaaacgaca
      541 cgagtcaagc ctagctcttt ccagaactca agggtttcat agttagtggc actggcttgg
      601 gtagaaaggt ggatttcaag gcctggtgct tcagtcgctg caatcataat caaggctggg
      661 tcagatacga taactgctgc aatcccgata tcacgcagtt tacggaacca ctcaccagca
      721 ccagcttcat ttccttcgtg cataaccata ttagccgcta catagacctt ggcaccatac
      781 ttggccgcaa actgcacgcc ttcttccatc tgttcgaaag taaagtttcc cgcacggcta
      841 cgaagaccat aggcctgacc accgataaag acagcatctg ctccatactg aacagctacc
      901 tttagcttct ctaaagtccc tgcaggtgat aaaacctcag gacgttttaa tgtttttgtc
      961 ataatttctc ctatttgcaa tataaaagtt tgacattttt ccttctcatt ttatagtaaa
     1021 taaaggataa aatcaagcct agacggattg ttttgacaat tctaatcttt tagactttgc
     1081 ataataacta ttaaacagtc gaaaattacc ccagttttaa caagtagtca aaaagtctaa
     1141 aaaagtaaaa accagtatcc caagatactg actcgtaaaa atatataaat tagtccttag
     1201 atgactactc ccactctgtt gctcctataa tggaaaagga ttgatacagt ctagaatatc
     1261 gcttttttat ttattattat gatataaaac tgttagaatt gattttttgt tttataccca
     1321 aatatgaata gagacgtagc taataagttg aataacatat ttggaaaaat gagaaatagc
     1381 aatattacta gaacttttag aaacaatcaa tatttaaaaa gaaaaaagct caaagaattt
     1441 attaaaccat gttataatag atatagctat aattttattt ataacaagag gatagaaatg
     1501 accgaattag aaagaaaaaa tcgaaaaatt agctaagaaa tattctgata acttaaacat
     1561 caaagttcaa gagagagttc gtgaaatggc aaatgataat aagagccatt atttgatata
     1621 cagagtttta ggtatttcat ttgaagaagg agaaaatatc gatttgtatc aaaataaagg
     1681 tcgtttttta tacaaatatg ctggttcatt tttagaagaa gctgcagtac tatcctttaa
     1741 cgaaaaattt ggtacagaaa atacttaaaa agttaacatt cctaattctg aaagtacaaa
     1801 acctaagact tttgaaattg attgtttagt cggagaaaaa cacgcatacg aaataaaatg
     1861 gtgggatgca actacagatg gagaccatat aactaaagaa cacactagaa taaaagttat
     1921 tcataacaaa ggatatatac caattcggtt aatgttctac tatccaaata gaactcaagc
     1981 tataaaaatt cagcaaactt tagaaacatt gtataacggt attggaggga aatattatgg
     2041 agattctgcc tgggaacatt taagagcagt gaccggtatt gatttactta gtattctaac
     2101 agatattgca aataaaaaac aggggtaaaa tcaaaatgac agtattaaaa ggagataact
     2161 tagaaatatt aaaaactatt gaatcctcaa gtattgattt aatctatatg gaccctcctt
     2221 tctttacaca gaaaacccaa aaattatcta ataacaaaaa tattatgtat tcattcgaag
     2281 atacgtggac ttcgattgag gattacaaag aatttttgtc tgtaagatta gaagaatgca
     2341 aaagagtgct aaaaaatagt ggcagtattt tcgttcattg tgataaaatt gcaaatcatc
     2401 atattagatt aattttagat aatatctttg gagcagatat gtttcaaagc gaaattatat
     2461 ggaactataa acggtggtct aattcaaaaa agggattatt gaacaatcat caaaacattt
     2521 acttttattc aaagtcaaaa gattttaaat ttaatacaat ttttacagag tattcttcta
     2581 ctacaaatat cgaccaaata ctagtggaac gaaaacgaga tggaaactct aaaactatat
     2641 ataaggttga taataatggt aactatattc tagcaaaaga gaaaaatgga gttccccttt
     2701 cagatgtttg gaatatacca tttcttaatc caaaagctaa agaaagagta ggttatccta
     2761 cacaaaaacc tattctgtta ttagaacaaa ttataaagat tgctactgat aaaaatgata
     2821 tagttttaga cccgttctgt ggaagtggaa ctactttagt agcctccaag attttgaata
     2881 gaaattatat ggggattgat ttatctgagg aagctatcaa tataactcag caacgtctgg
     2941 aaaatgttat aaaaacaagt tcaaatttat tgaataaagg aatcgaagca tatagaacca
     3001 aaactgagga agaggaaaac attcttaaat tattacaggc aaaaattgtt caaagaaata
     3061 aaggaattga tggtttttta cctaaacatt ttcaaaaaaa accgatacct ataaaaattc
     3121 aaaaaaataa tgaatgtctg aatgagagta tctctttatt acagaatgct ataaactcca
     3181 aaaaacttga ttttggagta gttataaaaa ctcattcaga taatttatta tttgattttg
     3241 atacgattcc tgaaaatata atagtagttg accattttga acttacaatc gaaaaatggt
     3301 taagtaaaag tcaacagtta ctatagtttg caaattattt acagaaagaa caaaaaatgc
     3361 tctgaaacac tgatttcaag gcattttttg ttagtttaaa attactccca ttcttctttt
     3421 ccaaacatac aatatatcca aaaccattca aaatactaga ttctattttt tataatatca
     3481 ctaaatccac ctaattatag gacgttttca gatttttagt cccgtcccag tcccagtacc
     3541 ggagaaatat tgttttaata taatatctct ttttgtcttc taagctctta aaagcaaaag
     3601 aacaagtaaa gagtcaagac aaggataaaa agtccatatt agggcaaata aaaagcttta
     3661 agacagatga caaatctaag tcaaataaga aagaccatag caaaggtgca gagagataaa
     3721 tattggcggt cttcggactg cctttatttt tttatccatt tttcaaatca aatttattca
     3781 gactatatat gcacatatac acttaaattc atataaaaac atggcttgta aaaaattact
     3841 ttaatcacaa taatcgcatt taaaattgtg atgtttgcaa gctaaattac ggacttcact
     3901 tggaagtttt cccttgtatc ttttataata gatagaaaat ttgctggcag atgaatatcc
     3961 aacagattct gctatctctt ttataggtag ttcagtgttt aaaagaagag tttcagctac
     4021 attcattctt tttctttgag tgtactctgt aatgctttga caatattttt ccttaaataa
     4081 attttttaat ttagtaccac tcattttaga tattttttca agcgtgcctt gatttacatt
     4141 cgttgcaaaa tgatcatcta agaatcttgc tacatcttca agtgctttat catcatcaat
     4201 ttcaatttta tatttttttc tatttaagta tgtgtcaatt actatactta tccattcatt
     4261 tgcctttgct ttaaagaaaa aatcagcggc aggagcgtcc atcttacaat ttaatatttc
     4321 cattgccact ctttctaagg cctttgtaag tattatttga ttcggttgaa gcaaggttga
     4381 ataaaaagat tctggattaa tgttaataga tgctaaatgt ttttctatta gctctttttt
     4441 aaaaccaatg gaaacagcaa gataacaaca attctcgtgt aataaaaaaa caaaatcatc
     4501 ttttatatta tcaaaatcaa aagtacataa agagtttgcg gtaatagttt gatacggatt
     4561 aaacttttct ccgtttgcac tgacaatgta acttgaataa attgaaacat agtctgacat
     4621 actataagtg ctattttgaa ctacttcctc tttgatataa aaatcatgta tatcgataat
     4681 gaagctgcct ccttcataaa accaatatag gccttctgca taagttgaat tctctttttt
     4741 ccaacaaaat gtatgacctg cacttgaata cttctcattg tttttacatt catctacttt
     4801 catataatct tttacaaaat cataatatga cataacacac tatccctttt agacaatatt
     4861 ccaattagcc ttattaattc aaaactattg tattagtaat tataacagat gtataataga
     4921 aaagcaatga tagatattat caattaagcg aatttatatc taaaagggat attaaagaaa
     4981 ggagatatgc ttatgaagat ttacaaaaaa ctatttgctt atgtccaaga taagaaatat
     5041 cttggggttt tggccataat tttttctgct atatctgctc tacttacagt atatggatat
     5101 tatttaatct acaaatttct agataagtta ataattaatt caaatttatc cggtgcagag
     5161 agcatagcat taaaatctgt tattacacta acaagtggag cgatatttta ttttgtctca
     5221 ggaatgtttt cacatatctt gggattcagg cttgaaacaa atttaagaaa aaggggaatc
     5281 gatggtctgg aaaaagcaag ttttaggttc tttgacttaa atccatctgg tcaaataaga
     5341 aagattatag atgacaatgc tgcacaaact catcaggtgg tagcacacat gattcccgat
     5401 agttctcagg caataatcac acccgtactt gtacttgcac ttggctttat agtaagtata
     5461 agagttggca taattttgct tgctcttact ataattggtg gcttaatttt aggggcgatg
     5521 atgggcgagc aagaatttat gaagatatac caagaatccc tatctaaact aagtgctgaa
     5581 actgttgagt acgtgagagg aatgcaagtt gtaaaaatat ttaaagcaaa tgtagagtct
     5641 tttaaaagct tttataaggc gataaaagat tactcaaagt atgcttatga ttattcccta
     5701 tcttgtaaaa ggccttatgt tttgtatcaa tggttatttt ttggactgat tgcaatttta
     5761 attattccta tagtttattt tatgactagc ttagctagcg caaaggtgat tttacttgag
     5821 cttatcatga ttttattttt atcaggagtt ctctttgttt cattcatgag aatgatgtgg
     5881 tactccatgt atatttctca aggaaattat gcagtagata ctttagaggc gctttacgaa
     5941 gatatgcaaa aagacaaatt agtgcatggt aatatcaata attttaaaaa ctataatata
     6001 gaatttgaga atgttagctt tgcttataat gataaagctg tcattgaaaa tttatccttt
     6061 aatttagaag aaggaaagtc ctacgcactt gtcggttcat ctggatcagg caaatcaaca
     6121 gtagcaaaac ttatatcagg tttttataat gttaataaag gaagcataaa gataggcggg
     6181 atagcaataa gtgaatattc tgacgaagcc ttaattaaag ccatttcctt tgtttttcaa
     6241 gattcaaaat tattcaagaa gagcatttat gataatgtag ctttagctaa taaagatgcg
     6301 acgaaagatg acgttatgag agccttaaaa ttagcaggat gcgatttaat attagacaaa
     6361 ttcccagaaa gagaaaatac aatcataggc tcaaaaggtg tttatttatc cggtggagaa
     6421 aaacaaagaa ttgcaattgc tagagcaatt ttaaaggatt ccaaaattat tattatggat
     6481 gaagcatcag catctattga cccagataac gagtttgaat tgcaaaaagc ttttaaaaat
     6541 cttatgaagg ataaaacagt tatcatgatt gcacacaggc tatctacaat taaagacctt
     6601 gatgaaatta ttgtcatgga tagtggaaaa attatagaaa gagggtctga caaagaatta
     6661 atgtcaaaag atacaaggta taagagcctg caagagatgt ttaacagtgc gaatgaatgg
     6721 agggtttcaa atgaaagagt tttataaaaa aagatttgct cttacagatg gaggagcaag
     6781 aaatttaagt aaagcaacac tggcttcatt tttcgtttat tgtataaaca tgcttcctgc
     6841 catattactt atgatttttg ctcaggaagt tttggaaaat atgggcaaaa gcaatggctt
     6901 ttatatagta ttctcagttt tgactttgat agcaatgtat attttgcttt ctatcgaata
     6961 cgataaatta tataacacaa cctatcaaga aagtgcagat ttaagaataa ggacagcgga
     7021 gaatttatca aaattacctc tatcttactt ttctaaacat gacatttccg acatttcaca
     7081 aacaatcatg gctgatattg aaggcataga gcatgcaatg agccactcaa taccaaaggt
     7141 gggcggcatg gtactgtttt tcccattaat atctgtaatg atgctagcgg gcaatgtcaa
     7201 gatgggttta gctgtaatta tcccatctat tttaagcttt atatttatac ctttatctaa
     7261 aaaatatcag gttaatggac agaatagata ttatgatgtc ttaagaaaaa actcagaaag
     7321 ctttcaagaa aatatcgaaa tgcaaatgga gattaaagca tataatttat cgaaggatat
     7381 taaagatgac ttatataaaa aaatggaaga tagtgagaga gtacacttaa aggcggaagt
     7441 aactacaatt ttaactttgt ctatatcttc aatatttagc tttatatctc ttgctgttgt
     7501 gatatttgtc ggcgtaaatc taattattaa taaagagata aattctctct accttatagg
     7561 atatttacta gctgctatga agataaaaga ctctttagat gcatctaaag agggcttgat
     7621 ggaaatattt tatttatcgc ccaaaataga aagattaaaa gaaattcaaa atcaagattt
     7681 acaagaaggc gatgactata gcttaaaaaa tttgatattg atctaaaaga tgttgagttt
     7741 gcctacaata aagacgcaaa agttttaaat ggtgtaagtt ttaaagctaa gcagggagag
     7801 gtcactgctt tggtaggtgc aagtggctgc ggtaaaacaa ctatcttgaa acttatatca
     7861 agactttatg attatgacaa gggacaaatc ttaatcgatg gcaaagatat aaaagaaata
     7921 tcaacagaat ctctttttga taaggtctct atagttttcc aagatgtggt tctctttaat
     7981 caaagcgtta tggaaaatat tagaatcgga aagcaagatg caagtgacga agaggttaaa
     8041 agagcagcaa aacttgcaaa ttgcacagat tttatagaaa aaatggataa aggtttcgat
     8101 acagttattg gtgagaacgg agctgagcta tcaggaggag aaagacaaag attatcaata
     8161 gccagagcct tcttaaaaga tgcgccgata ttgatcttag atgagataac agcaagcctt
     8221 gatgttaaca acgagaaaaa gattcaagag tctttaaata atttagttaa agataaaact
     8281 gttgtaatca tttcacatag aatgaaatcc atagaaaatg cagacaagat agtagttctt
     8341 caaaacggaa gagtagaaag cgaaggtaag catgaagagc ttttacaaaa atcaaaaatt
     8401 tacaaaaatt taatagaaaa gacaaaaatg gcagaagaat ttatttatta ggaggactac
     8461 aatggataat aaaaaattaa aagtaaaaga tttagtaagc atcggtgttt ttggcgtaat
     8521 ttattttgcc ttcatgtttg gagttggtat gatgggcttg attccaatat tgttcttaat
     8581 atacccgaca gtattagcca tagttgcagg aactgttgtt atgttattta tggctaaggt
     8641 tcaaaagcca tgggcactat ttatatttgg tatgatatca ccacttgtga tgtttgcagc
     8701 tggtcatacc tacgtagttg tggttttatc acttatagta atgataatag cagaattaat
     8761 tagaaagatt ggtaattata attcatttaa atacaatatg ctttcttatg caatcttcag
     8821 cacatggata tgtagctctt taatgcaaat gcttttagca aaagaaaaat atatggagtg
     8881 gtctttgatg acgatgggaa aagattatgt tgatgtgtta gaaaagttaa taacttatcc
     8941 tcatatggct ttagtagcct taggtgcttt cttaggagga attcttggag catatatagg
     9001 caaggctcta ttgaaaaaac acttttcaaa tggatcatat tgtgtgggat actttactcc
     9061 ttgcctaatt ttatggtgtt atctgaatta aaccctatag ttaagatgtt tttgagtata
     9121 cctattgtta ttagaatgtt tattttacca tttatagcag caagctttat gataaagacc
     9181 tcggatgtag gcgcaataat ttcatcgatg gataagctta agatttcaaa gaatgtatcc
     9241 atacctattg cggttatgtt tagattcttc ccatctttta aggaggagaa gaaaaacatc
     9301 aaaatggcta tgagagtaag agggataagt tttaaaaacc cagtcaaata tcttgaatat
     9361 gtttctatgc cactactcat tatatcatct aatatatcag atgacattgc aaaagcggca
     9421 gaaacaaagg caatagaaaa tccaattgcc aagaccagat acattcgcgt aaagatacag
     9481 ctaattgatt ttgtttatgt tttagcggtt gctggactta ttgtgggagg cttaatatgg
     9541 ttgaaataaa aaatttaagt cttgattatg gtgaagagcg tatattagat gatatatcac
     9601 tatccatagc cgagggagag tgcgtgctat ttacaggaaa aagtggaaat ggtaagtcat
     9661 ctttaataaa ttcaatcaat ggactagctg taaggtatga taacgcaaag acaaagggcg
     9721 aaataattat tgatggtaag aatataaaaa atttggaact ttatcaaatc tcaatgcttg
     9781 tttcaactgt ttttcaaaat cctaagacct atttttttaa tgtcaatacg acattagaat
     9841 tattatttta tttggaaaat atcggtcttg caagagaaga gatggacagg cgtttgaagg
     9901 atatgcttaa gatattcccg ataaaaaatc ttttgaacag aaatatattt aatctatccg
     9961 gcggtgaaaa acaaattctt tgcattgcag cttcttatat agcaggtaca aagattatag
    10021 ttatggatga gccttcatcg aatttagata ttaaaagcat aagtgttttg gcaaagatgc
    10081 taaagatatt aaaagagaaa ggtataagca taattgttgc agagcataga atttattatt
    10141 tgatggacat agttgaccgt gtatttttaa tagataaagg aaagcttaaa aaaacttata
    10201 ctagaagtga atttttaaag ctagataaaa atgaattaaa tgctttaagt ttaagagata
    10261 aagaattaag taaattaaaa gttccttatt taaaagaagg tggagagtat cagataaaaa
    10321 atcttagcta caaatttact gatgatgagt gtttaagctt aaaagatatt tcattcaagc
    10381 ttgggaaaat ttatggcata ataggatcca acggacgagg aaaatcaacg cttttaagat
    10441 gtttaatagg tcttgagaaa aaatcaaaag aagaaattta ttttaaggga gagaagctat
    10501 ctaaaaaaga aagactcaaa aactcttcac ttgttatgca agatgtaaat catcaattat
    10561 tcacagatga agtattcaac gagcttagat taggagtaaa gaattttgat gaagaaaagg
    10621 cgaaaatcat tttaaaagat ttaggcctgg acgaatttat tgaaaggcat ccgatgagtt
    10681 tatcaggagg gcaaaagcaa aggtttgcaa tagcatctgt tatgtgcaag acttctccat
    10741 ttgtcttttt tgacgaacct tcaagtggta tggattattc caatatgata aaaatatctg
    10801 aactgattaa taagtataaa accatggata aaataatttt tattgtttcc catgatatag
    10861 aatttttaaa tgaagtggca gatgaaattt ttgaattgta aaatagagtc tgttaaacat
    10921 gtctatagaa ctagtaaatt gtctaaaaat ttaaaagata ctaacacata ttgatattta
    10981 ttagaaaatt aacttcagga tttttacagt atttataaac tttataatat gtatttatag
    11041 tattaataat ttccatatct ttagggaaat tatttattta cttaccataa ttaaagagat
    11101 gagagataaa tatcggcagt cttcggactg cctttatttt tttacttaat ttaatgcagt
    11161 aaatcatacg attgaaagga atctgcgtta tatgctataa ttaggtgatt aatctataca
    11221 tttagaaagg taaaatatag aattggagaa tgatatggga ttttcatata ataaattgta
    11281 accgtccaat aacgaagtat attgaaaaat ctccagacta gagaactcac ggatagttcc
    11341 taatctggag atttcttatt tgcacttttc ttgtacaact ttagtccacg gtaaataggc
    11401 ctctaaaacc tctttgttta cgagagtttc ctcgtttgga agacattcta gaagatagga
    11461 tagatatttc tcgctattta taatggattg aaataagata tgaacaaatc gattaggaaa
    11521 ggaaaattga tttatagaaa tattttagca gtcgatgcgt actgttatag attcaacgca
    11581 ctataattga tgacgtttag ctgtttccaa caaactcata ataatagctc ttgctttagc
    11641 tccttcaaaa ctttgagaaa ataaccaccc tcgctttttt aaactgagct caggctaaaa
    11701 gagtccactg gactctttta ctccgtccca taaccaatga tttaatagcg cgttcagcta
    11761 gattattgga aaggaccaga tgtccgtctt ttaaaatggt cttaaaggtt tctttatact
    11821 tgaggctgta ttcaattgcc cttcctagtt ttgaacctgc taaaactgac tgacggcggc
    11881 accaagcaaa gaagtcttcc attaggggct ggacgtttct gtagtcgttc atcagctggc
    11941 aaagcctccc agtctctttc caaggcaaat aactggtcac gataggctaa acctttagct
    12001 cctaaggatg atttatctgc ttgcttgggg gtcgcttcaa aaaatttcct tctcacatgc
    12061 gcccaacatc caacaagttc agcttcttcc agttgacgat aagcttctag cggttcgcag
    12121 tttaccaagc ttagcgtcgc cttgctccta aaccttagac tgctatcgca taggcagaac
    12181 tagaggacta aagttctaag ttactgccgc aacatatcac aatgcatata gccagaataa
    12241 tttcctagga attcttgcac taccgaacca ctccgacgct gatcatggtg gtacagcgta
    12301 atcccttgtt tctcagcttt cccagacaaa aaagtccaat agtaagtcag ctgactatca
    12361 ctttctaaga cccgataaga ggtttcatct gcatgaagaa gagcttgttc taacaacttt
    12421 tctcgtaaaa ggttataaag ggactctaaa tagtattgac tcgccttgat atgccaatta
    12481 gcaatttcct tacgtgtgat tggtaaaccc atcttagccc aatcttcttc ttagcgataa
    12541 ttgggtacct tcagaataaa cttctgatgg atggtgtgag cgataataga agctgagcca
    12601 aggctatgcg ccaaaggggc tttaggaata ggagctttca cgattttatc acttggatta
    12661 ttcttactgc atgtttggca tttgtaagcg tgttggatat gattcactct ttttaattgc
    12721 gcaagaataa agaccaattc ttgtcgttga aggctcgctc caatctcttt tagctctccc
    12781 tgacagtcag ggcaaatgca gttctctaag cgatgatgaa cttcttctga atcaaattgg
    12841 gcaagaatag cttgacgttt ccctttagct ttcttacgtt tataggtgat ttcttctctt
    12901 tcaactgggt aagtcagagt cttcttccat attttgttcc tcttcaaaaa gactgagttg
    12961 tccagatggg caaacacttt tctcagagga ttttccatag agcttttgcg ttagataagc
    13021 cacttgttca cgaagaaggg caagttcatt ggtgagacta tcaattgtag cactctgttg
    13081 ttgaatgatt ttcaatcttc tcccatagac tttcctcctt atttcctaac tttattatat
    13141 agtgtattga atctataaca gtacaccttg actgctaaaa tatttctata aattaatttg
    13201 actttcctga tagagatgtt catatcttat ttcaattcac tatactaaaa agaaagtcct
    13261 catttcaata gaaatcacga ctttctgatg aatttatttt tggagtgata gaaaagccct
    13321 tcataagcca gtctacttgt tcaggtgtga gagctttgac atccttttct gtactgggtc
    13381 aagtcagtct gccgttctca aagcgtttat atagtagcca aaatccttga ccatcccagt
    13441 aaagggcttt aaagcggtct ttacgtccac cacaaaagag aaaaacttga ccggagaaag
    13501 gatctaattc aaagtgggtt ttaaccagat aagccagtga atcaatgcct tgcctcatat
    13561 ccgttttccc acatacgaga tagacctgcc ctaggctaga tagatgaatt gtcatagagc
    13621 aacaccttat ccaaaagctg ttctaccatt tcgagattga gagattgaaa aaaacttact
    13681 tc
//
LOCUS       DAVDWM010000052        13580 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVDWM010000052 DAVDWM010000000
VERSION     DAVDWM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.052"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(196..273)
                     /locus_tag="VUW49_001798"
                     /pseudo
     CDS             complement(196..273)
                     /locus_tag="VUW49_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            344..1000
                     /locus_tag="VUW49_001799"
     CDS             344..1000
                     /locus_tag="VUW49_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET7981675.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVMSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            990..1199
                     /locus_tag="VUW49_001800"
     CDS             990..1199
                     /locus_tag="VUW49_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET7981676.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIK"
     gene            1417..2247
                     /locus_tag="VUW49_001801"
     CDS             1417..2247
                     /locus_tag="VUW49_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7981677.1"
                     /translation="MKKIVKYSSLAVLALVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2401..3255
                     /locus_tag="VUW49_001802"
     CDS             2401..3255
                     /locus_tag="VUW49_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7981678.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKSLFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3355..4728
                     /locus_tag="VUW49_001803"
     CDS             3355..4728
                     /locus_tag="VUW49_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7981679.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4721..5782
                     /locus_tag="VUW49_001804"
     CDS             4721..5782
                     /locus_tag="VUW49_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET7981680.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5784..6476
                     /locus_tag="VUW49_001805"
     CDS             5784..6476
                     /locus_tag="VUW49_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981681.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6506..7075)
                     /locus_tag="VUW49_001806"
     CDS             complement(6506..7075)
                     /locus_tag="VUW49_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET7981682.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7210..7824
                     /locus_tag="VUW49_001807"
     CDS             7210..7824
                     /locus_tag="VUW49_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET7981683.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7826..9472
                     /locus_tag="VUW49_001808"
     CDS             7826..9472
                     /locus_tag="VUW49_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7981684.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9484..10770
                     /locus_tag="VUW49_001809"
     CDS             9484..10770
                     /locus_tag="VUW49_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981685.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGHPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10822..11355
                     /locus_tag="VUW49_001810"
     CDS             10822..11355
                     /locus_tag="VUW49_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981686.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11433..11903
                     /gene="nrdI"
                     /locus_tag="VUW49_001811"
     CDS             11433..11903
                     /gene="nrdI"
                     /locus_tag="VUW49_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET7981687.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12196..13458)
                     /locus_tag="VUW49_001812"
     CDS             complement(12196..13458)
                     /locus_tag="VUW49_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET7981688.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3954 a         2543 c         3113 g         3970 t
ORIGIN      
        1 aatagtccat gctgtggttg gattcttttc agcctacttg tactcttcga aactctcttc
       61 aaaccacgtc agcttcactt tgccgtaggt atggttactg acttcgtcag ttctatccac
      121 aacctcaaaa cggtgttttg agctgacttc gtcagttcta tccacaacct caaaacagtg
      181 ttttgagctg acttcatcag ttctatctgc aacctcaaaa cagtgttttg agcaacctgc
      241 ggctagtttc ctagtttgct ctttgatttt cattgagtat tattaagaca atatgaggag
      301 aaagatgaaa gaaaaaggat tttaggaggg ggcgcaggca gccatgccaa cggctcttgg
      361 ctatgtcagt atcggcctgg cctgtggaat tatcggtgcg ccctatgtga cacctgttga
      421 gatgggcttg atgagtctct ttgtttatgc tgggagtgcc cagtttgcca tgttggcact
      481 gattgtggtt caagctcctg tggcagctat tgctatgacg gtttttctaa tcaacttgcg
      541 tctctttttg ttgagtttac acgcatcgac ttatttccgt cataccagtc tttggtacaa
      601 tatcggtatg tctagtatct tgacagatga gacctatggc gttttgatgg gtgaattggc
      661 ccatacagac aaggtaaatc ctatgtggat gcacggaaac aatcttaaca gctatgtggc
      721 ttggtttgtg gggacagtag tcggaacggc tctgggtggc ctgctaccaa atccagaaat
      781 ctttggcttg gattttgccc tggttgggat gtttattggt atttttgctt cgcaatttca
      841 gattatgcaa agacggattc ctgtccgcaa tctgctcatt atcctagcag ttgttgcggt
      901 gtccttcttt ttgctcttga cagtgatgtc tcagtcacta gctgttctgt ttgcgacgct
      961 acttggttgt agcatggggg tggttttaga tggtcagtaa gtatctttta ttagcagtta
     1021 ttttctctgg cttggtgact tggattcccc gtatgattcc cttcatcttg gtcaagtata
     1081 agggcctgcc tgcgattgtt gagcgttttt tgaagttttt gcctgtttcc attatctttg
     1141 ccctgattct ttcaagcgta gtgacaggta aggttggcag tcttcctcaa atcaaatgac
     1201 tggacttctt agccgtcttt ccaacagctt gggtagcctt tcgctaccgc aatctagtcg
     1261 gaacagttct ctttggagtg gtcttgattg ccatcttgcg tttggtcttt taaattaccc
     1321 ataaaaaaaa cttatcacag agatagatat catataatgg cgtaaatgct ctttttctgt
     1381 taagattata aggtattcta ttttggagga aatgacatga aaaaaatcgt taaatactca
     1441 tctcttgcag tccttgctct tgttgctgca ggtgtgcttg cggcttgctc aggtggtgct
     1501 aagaaagaag gagaagcagc tagcaagaaa gaaatcatcg ttgcaaccaa tggatcacca
     1561 aagccattta tctatgaaga aaatggcgaa ttgactggtt atgagattga agtcgttcgc
     1621 gctatcttta aagattctga caaatatgat gtcaagtttg aaaagacaga atggtcaggt
     1681 gtctttgctg gtcttgacgc tgatcgttac aatatggctg tcaacaatct tagctacact
     1741 aaagaacgtg cggagaaata cctctatgcc gcaccaattg cccaaaatcc taatgtcctt
     1801 gtcgtgaaga aagatgactc tagtatcaag tctctcgatg atatcggtgg aaaatcgacg
     1861 gaagtcgttc aagccactac atcagctaag cagttagaag catacaatgc tgaacacacg
     1921 gacaacccaa ctatccttaa ctatactaag gcagacttcc aacaaatcat ggtacgtttg
     1981 agcgatggac aatttgacta taagattttt gataaaatcg gtgttgaaac agtgatcaag
     2041 aaccaaggtt tggacaactt gaaagttatc gaacttccaa gcgaccaaca accgtacgtt
     2101 tacccacttc ttgctcaggg tcaagatgag ttgaaatcgt ttgtagacaa acgcatcaaa
     2161 gaactttata aagatggaac tcttgaaaaa ttgtctaaac aattcttcgg agacacttat
     2221 ctaccggcag aagctgatat taaataattt cttgaaatca tccattctga gtaaggtggg
     2281 tggtttctca gtttttaggg atatagtttt aaactatata tctgactatc taataacaat
     2341 ttgtgaaatc aaacaaattg tgagatacta gtacggtatt atttttaagg agaaagaatc
     2401 atgaaaatca aaaaatggct tggtctagca gcccttgcta cagtcgcagg tttggctctt
     2461 gcagcttgcg gaaactcaga aaagaaagca gacaatgcaa caactatcaa aatcgcaact
     2521 gttaaccgta gcggttctga agaaaaacgt tgggacaaaa tccaagaatt ggttaaaaaa
     2581 gacggaatta ccttggaatt tacagagttc acagactact cacaaccaaa caaagcaact
     2641 gctgatggcg aagtagattt gaacgctttc caacactata acttcttgaa caactggaac
     2701 aaagaaaacg gaaaagacct tgtagcgatt gcagatactt acatctctcc aatccgcctt
     2761 tactcaggtt tgaatggaag tgccaacaag tacactaaag tagaagacat cccagcaaac
     2821 ggagaaatcg ctgtaccgaa tgacgctaca aacgaaagcc gtgcgcttta tttgcttcaa
     2881 tcagctggct tgattaaatt ggatgtttct ggaactgctc ttgcaacagt tgccaacatc
     2941 aaagaaaatc caaagaactt gaaaatcact gaattggacg ctagccaaac agctcgttca
     3001 ttgtcatcag ttgacgctgc cgttgtaaac aataccttcg ttacagaagc aaaattggac
     3061 tacaagaaat cacttttcaa agaacaagct gatgaaaact caaaacaatg gtacaacatc
     3121 attgttgcaa aaaaagattg ggaaacatca cctaaggctg atgctatcaa gaaagtaatc
     3181 gcagcttacc acacagatga cgtgaaaaaa gttatcgaag aatcatcaga tggtttggat
     3241 caaccagttt ggtaataaga aacagggagg tgggagagaa aattccacct cttgcttttg
     3301 tatagagtat agattgtaaa gaagactatt cgttcataga aaggtagaga gaatatggtt
     3361 tttcctagcg aacaagaaca gattgaaaaa tttgaaaagg atcatgtagc ccagcattat
     3421 tttgaggttt tgcgtacctt gatttctaag aaatcagtct ttgcccagca ggttggactc
     3481 aaggaagtcg caaattatct gggtgagatt ttcaagcgtg ttggagctga agtggagatt
     3541 gatgagagct atacagcgcc ctttgtcatg gcacatttca agagttcgcg tccagatgcc
     3601 aagaccttga ttttctataa ccactatgac actgtgccag cggatgggga tcaggtctgg
     3661 acagaggatc cttttacgct ttcggtccgc aatggcttca tgtatgggcg tggggttgat
     3721 gacgacaagg gtcatatcac agctcgcttg agtgctttga gaaaatatat gcagcaccat
     3781 gatgatttac ctgtcaatat cagctttatc atggagggag cggaggaatc ggcttcaaca
     3841 gacctagata agtatttgga aaagcatgca gacaaactcc gtggggcgga tttgttggtc
     3901 tgggaacaag ggaccaaaaa tgccttggaa cagctggaaa tttctggtgg caataagggg
     3961 attgtgacct ttgatgccaa ggtaaaaagc gctgatgtgg atatccactc gagttatggt
     4021 ggtgttgtgg aatcagctcc ttggtatctc ctccaagcct tacagtctct tcgtgccgcg
     4081 gatggccgta tcttggttga aggcttgtac gaagaagtac aagagcccaa tgaacgagaa
     4141 atggccttgc tagaaactta tggtcaacga aacccagagg aagttagtcg gatttatgga
     4201 ttggagttgc ctctcttaca ggaggagcgg atggcctttc taaaacgttt ctttttcgag
     4261 ccagcgctta atatcgaagg aatccagtct ggttatcaag gtcagggtgt taagactatt
     4321 ttacctgcag aagccagtgc caagctagag gttcgtctgg ttccgggcct agaaccgcat
     4381 gatgttctgg aaaaaattcg gaaacagcta gacaaaaatg gctttgataa ggtagaatta
     4441 tactatacct tgggagagat gagctatcga agcgatatga gcgcaccagc cattctcaat
     4501 gtgatcgagt tggccaagaa attctatcca cagggcgttt cagtcttgcc gacgacagcg
     4561 gggacaggac ctatgcatac ggtctttgat gccctagagg taccaatggt tgcattcggt
     4621 ctaggaaatg ccaatagccg agaccacggt ggagatgaaa atgtgcgaat cgctgattat
     4681 tacacccata tcgaattagt agaggagctg attagaagct atgagtagag atattatcaa
     4741 gttagatcag atcgatgtga cttttcacca aaagaagaga accatcacag cggttaagga
     4801 tgtgaccatt cacatccaag aaggggatat ctacggaatc gttggatatt ctggagcagg
     4861 gaaatcaacc cttgtacggg tgattaatct cttgcaaaaa ccatctgcag ggaaaattac
     4921 cattgacgac gatgtgattt ttgatggtaa ggtcaccttg acggcagagc agttgcgtcg
     4981 taaacgtcaa gatatcggga tgattttcca gcattttaac ctgatgagcc aaaagacagc
     5041 agaggagaat gtagcctttg cccttaaaca ctctggactc agcaaggaag aaaagaaggc
     5101 taaagtagct aagttgttgg acttggttgg tttggcagat cgtgctgaaa actacccttc
     5161 acaactatct ggagggcaaa aacagcgtgt ggcaattgcg cgtgccttgg ccaatgatcc
     5221 aaaaatcttg atttcagacg agtcaacttc tgcccttgac cctaagacaa ccaagcagat
     5281 tttggccttg ttgcaagatt tgaaccaaaa attaggcttg actgttgtct tgattacgca
     5341 tgaaatgcag attgtcaaag acattgccaa ccgtgttgca gttatgcagg atgggcattt
     5401 gattgaagag ggtagtgtgc ttgaaatctt ctcaaaccct aaacaacctt tgactcaaga
     5461 ctttatctca acagctacag gtattgacga agccatggtc aaaatcgaga agcaagaaat
     5521 cgtggaacac ttgtctgaaa acagtctctt ggtgcaactc aagtacgctg gagcttcaac
     5581 agacgagcca cttttgaatg aattgtacaa gcattaccaa gtaatggcta atattctcta
     5641 tgggaatatc gaaattctcg atggtactcc tgttggagaa ttggtggtgg ttttgtcagg
     5701 tgaaaaagca gcgttggcag gtgcccaaga agccattcgt caagcaggtg tacaactaaa
     5761 agtattgaag ggagtacagt aagatggaat cattgattca aacctattta ccaaatgtct
     5821 ataagatggg ttgggctggt caggcaggct ggggaacggc tatctactta actctttata
     5881 tgacagttct ttccttcatt atcggaggct tcttggggct agtggcaggt ctctttctcg
     5941 tcttgacagc gccaggtggt gtcttggaga ataaagtcgt attctggatt ttagacaaaa
     6001 ttacctcaat ttttcgtgcg gttcccttta tcatcctctt ggcaatcttg tcaccacttt
     6061 ctcacttgat tgttaaaaca agtatcgggc caaatgcagc ccttgtccca ctttcttttg
     6121 cagtctttgc cttctttgcc cgtcaggtgc aggttgtctt ggctgaattg gatggcggtg
     6181 tcattgaggc ggctcaagcg agcggagcga ctttctggga catcgtgggt gtttacctat
     6241 cagaaggtct tccagatttg atccgtgtga cgactgtgac cttgatttcc cttgttgggg
     6301 aaacagctat ggccggtgcg gttggagctg gtggtatcgg taacgtagcc atcgcttatg
     6361 gatttaaccg ctacaatcac gatgtgacca tcttggcaac cattattatc attttgatta
     6421 tctttgcaat ccaattcttg ggagatttct tgactaagaa attgagccat aaataaaaaa
     6481 gagccgcgtg gctcttttta actgatcaga ttttctgggc aaatttttta ctcaaggctt
     6541 gtccaatcaa ggcacccact agggctccga tgacaatact tgcgataaat agaaggacag
     6601 ttccagggtt tggagcgacc atgatacggt cgatatattc ttgggatttt cctcttgcca
     6661 gaagagtagc catataggct ttgggcgcaa tccacataag caagattggt cctgttgtac
     6721 taaaggcgaa aataatgaaa gaaaggaagt tctttgtttt gtccttgtat tttcctaaat
     6781 gagctactcc atctgctagg aggccacaga taattccagg aaggaaggaa ccagcaccgt
     6841 gtttagttcc caagaaaaag agggcaatga caaggccgat agtggtaatg gctccaaagc
     6901 gcggaacttt tgcgactagg atcatataga cgctaccgcc gacaagggca gtaaaggcag
     6961 gcgcataaaa catgtttcca gtttggtcaa agagattgcc caaaaggaca ccaatcccca
     7021 tgcagaggaa gtaaagaact gcaaaaagca gtgtagttaa gatagatttc ttcatgaatt
     7081 gtctcctgat aattttttca caattctcat tttatcatgg tttggtggga ttgtaaatgg
     7141 atactagaag tttttgaaaa cgcttgaaat atgatataat ggtttcatag ttatttttag
     7201 gggaatatca tgggtagatt tttagacttt gtctttaatc gtttcttttt agggatgatt
     7261 gcgacagcct tcttttggct attaacttta gcaggaggga ttatccttgg tctagcgccg
     7321 gctagtgcca ccttgatgag cttatatgca gaacatggtt atagctttcg ggaatacagt
     7381 ttgaaggagg cttggtctct ttacaagcaa aattttgtct caagcaacct gattttctat
     7441 agctttttag gtgtgggtct agttttgacc tatggtttgt atctcttggt gcaattgcct
     7501 catcagacca ttgttcattt gattgcgacc cttttgaatg tcctagtagt tgccctgatc
     7561 tttttggctt atacagtatc tttaaaatta caagtttatt ttgccttgtc ctatcgaaat
     7621 agtctcaaat tatccttgat tggcatcttt atgagtctag cagctgtggc taaggttctc
     7681 cttgggactg tgctacttgt agcaattggt tattatatgc ctgccctgct attttttgta
     7741 ggaattggga tgtggcattt ctttatcagt gatatgttgg aacctgtcta tgaaatcatc
     7801 catgaaaaat tggcgacaaa atagaatgaa gcagttttgg ctacatacgc ttctaagaac
     7861 ctatagttca gtgatgatca ttatcattgc gagttttgca atcttactct cttacgctga
     7921 ctgggattca cgtgaaaagg aagcccagag agtagcccag cgtgtaactg ctagaacagt
     7981 gagtgaaatt gaatattacc atagagagtc aacccagata gctcaggctt tagttgaaaa
     8041 tcaagctcgt attgagggaa tctataaata ctttagcctt agcatgccag actattttta
     8101 ctggcaatta gagcggaaag cttcgcctta tatatcagtc tctctgtatg aaaatgttga
     8161 tgacctctat gttcgaaatg attttgtaac tggagtggcc attgcttttc aagattacaa
     8221 ggaagtctat gtttctacta aagacaaacg tagtggagaa aaaatcaggg ctgaggattt
     8281 caaaccagca ggaaatagtt ttgccattcc ggtgtcagat ccagtgtcag atcaagattt
     8341 aggagtgatt tacatctcct tggatcctgc tgttttatac catgccattg ataatactag
     8401 aggtcatact ccgatggcag taacagtgac ctcacctttt gatacggaga tttttcatat
     8461 tggtgagaca gttgataagg agagtgaaaa ttggctagtt ggcttaactt ctcatggata
     8521 tcaggttcag gtggcagttc ctaaaaactt tgttttacaa ggaacagtga ctagctctgc
     8581 tttgattgtg ggtttgagcc ttctctttat tgtcattctt tatctgactt tgaggcagac
     8641 ttttgctaat taccaaaagc aggtagtgga tttagtagaa tccattcaag tcattgctca
     8701 aggcgaagag gggcgtcgga ttgacatttc cgagaaagat caggaattac tcctaatcgc
     8761 ggagacgacc aatgatatgt tggatcgatt ggaaaagaat atccatgata tttaccagtt
     8821 agagcttagt caaaaagatg ccaatatgcg agccttgcag gcgcaaatca atcctcattt
     8881 tatgtataat acgctggagt tcttgcgcat gtatgcagtt atgcagagtc aagatgagtt
     8941 ggcagatatc atttatgaat tcagtagtct cttgcgtaac aatatttccg acgaaagaga
     9001 gaccctcctc aaacaggaat tagaattttg ccgtaaatac agctatctct gcatggttcg
     9061 ctatcccaag tccattgcct atggtttcaa gatagatcca gagttagaga atatgaagat
     9121 tcccaagttt accttgcaac cgctggtaga aaactatttc gcgcatggtg ttgaccacag
     9181 gcggacagat aatgtgatta gcatcaaggc tcttaaacag gatggttttg tggaaatttt
     9241 ggtggtcgat aatggtagag gaatgtcggc tgaaaagttg gcaaatatcc gagaaaaatt
     9301 aagtcagaga tattttgaac accaagccag ctacagtgat caaaggcagt ctatcgggat
     9361 tgtcaatgta cacgagcgtt ttgtgctcta ttttggagac cgctatgcca ttactataga
     9421 gtctgcagag caagccggtg ttcagtatcg tattacaatt caagatgagt agaaagggag
     9481 aaaatgtata aagtattatt agtagatgat gagtacatgg tgacagaagg tctgaagcgt
     9541 ttgattccct ttgataagtg ggatatggag gtcgtcgcaa cagccagtca tgccgatgaa
     9601 gctctagaat atgttcagga aaatcctgtc gatgtcatca tttccgatgt caatatgcca
     9661 gacaaaacag ggcttgatat gattcgggag atgaaagaga tcttaccaga tgctgcctat
     9721 atcctgctct caggttatca ggagtttgat tatgtaaaaa gagcaatgaa ccttagtgtg
     9781 gtggactatt tggtcaagcc tgttgataag gtagagctgg gaaatctgct ggagaagatt
     9841 gcaggtcagc tcggcgagag agggaagaaa agtcagactc ttagtcaaga attagacgag
     9901 gctggatttg ttagttattt aggggataag gagaattggt ggataggtct atccaaggaa
     9961 aaacaaggtt ccttcaccat tccctactat gtcttgggtc aagactggca gattttcatt
    10021 tctggccacc ccctagatgg tttagtcgtt acaccttttg aagctcctta tcaagaacac
    10081 tttgaacgct ggaagctgaa tgctgagaaa accctctttt acggttctgt aaatctgcag
    10141 cagtctgaga gtctctttgc ctattacgaa ccgatttata gggttatcat tcagggaaat
    10201 ctcaatcaaa tcgtagaaga gttaaatctc ttggagaagg tagttcttga aaatacacct
    10261 cgtgtttcga ttactaaaca gctttttatc cagtttgtca tggatgtttt ccatttattt
    10321 gaacatctca aagctgatga tatgacggac attgtcaaaa ccattcatgc tattcaatcc
    10381 ttcgatgaat tggtttctta tatcaaggaa actctgatca gctttttcgg tcaataccgt
    10441 atgaatgaaa atgtggtcag tgtgctggaa gtcattggtc gtgattacca aaaagagctt
    10501 tccctcaagg atatcagtaa ggccctcttt atcaatcctg tctatctagg gcagttgatt
    10561 aagcgtgaaa ccgattcgac ctttgcagag ttactaaaca aacaacgtat taaggctgcc
    10621 cagcagctct tgctttcaac tagtgacagt atcgaagata tttgttatgc tgttggttac
    10681 agtaaccttg gatatttcta taaagttttc cgaaaattgt gcggaaaatc gccaaaagcc
    10741 taccgaaaac aggtagaaac tatactataa gatttgtatt cctttacaaa aggtgctata
    10801 atattatcag gaggtcctat catgaaaaag aaaccaattt atctatgggt tttgctaatc
    10861 ttgtctgccc ttatttcagt tccgtctctg tttggaattg tgagtccctt gcctagtaaa
    10921 gaagcccttc gtgctgctca gaaacaagtt gcaggggtca atgctcagca attagaagac
    10981 cagcttaatt atacctatag agtagcagaa gcatctcatt ctatttttaa tgttgctttg
    11041 attgtgctat ctactatttt agtggtcgta gcgattgtct tccttgttcg taaaaatttg
    11101 caatatgcaa actatactta cgttggctat gttttgttag ctattattgg ttcgatttat
    11161 ggctatgttg gtttacaaga cgctgtgcag ttggttcaag atgagagcat gcgtttgaca
    11221 gtgagtattg gttcaaaagc tgttagcatt ttctatatcg tcatcaatgt tctcttccta
    11281 gcccttgtct tctataagat gtggcgacaa cagaaagcct tggcagaaga agaggaaaca
    11341 gaagagctta cctaagtatt gacaaaaaag aactatcaag atacaatctt ggtagttgtt
    11401 ttttgattat agataaaatc atggaggtac atatgaagac aatttcttta gtttatatta
    11461 gtttgagtgg caacactgaa agttttgtga cgcgcttgaa agactatctc ttgtcccagt
    11521 acaaagggat tgaggttcaa aagattcata tcaaggattt ggtcaaggaa ggcaaaaatt
    11581 tctatgaaat ggaccatccc tatgtcgctt ttttgccgac ctatctcgaa ggtgggaatg
    11641 gcgtggataa cggagatgtt gagattttga cgacaccggt gggagatttt attgcctatg
    11701 gtaacaatgc tagtaagtgt tttggtgtgg ttgggtcagg aaatcgtaac tttaataacc
    11761 aatactgcct aacagccaag caatacagtc aacgctttgg cttccctgta ttggctgact
    11821 ttgaaatgcg aggcatgctg gaagatatca aacatgtcgc agctattatc gcagatttgt
    11881 atgagttgga aaaggagaat taatactcaa tgaaaatcaa agagcaaact aggaagctag
    11941 tcgcaggttg ctcaaagcac agctttgagg ttgcagatag agctgacgtg gtttgaagag
    12001 attctcgaat agtataaagt tcttataaga ggtggttgaa gttgttcaac tgttttttga
    12061 gtataaacag tctttaagaa cgtaaaaaag cataagttca ggtcttcaaa accttgttct
    12121 tatgcttttt atcatagaaa tatttgctga attttctctt gaaatgaaac cttatcttca
    12181 tctcgcgctt gtcccttacc ctgcttttcc gattgcgagt gcgtagataa agaagatggc
    12241 gaagccaaag aggaaaatca atcccgcaat ggcaaggtgt ttgagccaag aaggaagatt
    12301 tttgttttca cgcgttacca aggcgtaatt caaaagagca aagaaaggtg ttgtcaggaa
    12361 agagccaatc atggcaaagc ggagcatggt tgaaacctga ccagcgaaga acttgataat
    12421 gacgataccg atgatagcag tgatggtcat ccagatgttc aaagatttac gattgtcctc
    12481 tttttgactg attagcagtc ggagagattc ctgattaacg cgagaatagc catcgataac
    12541 agttataact gttccaaaga tacagaggaa ggcaataaag gtaatcaagt aacgggacca
    12601 ttcgccaaga acagaggcat acatgcccac gaattgagag atgtatttgg ctgaagcagc
    12661 ttcaaccgcc tgccctgtag gatactgaat cagtgctccc agtgccacaa agaagacggc
    12721 taggatagct gttccaatat aaccagtgtt aaagtcaaac agagcgtctt ctgtgttaaa
    12781 gttgacggtc tttctctttt cagctgacca aagtgaattg atggctgaaa tttcaatagg
    12841 agccggcatc catcctagga gggagacgat gaagggcaga gctgccattt gccaaggtgt
    12901 cttctcgaca aaatcagaac tgtattctgg atgcttgacc gccgcaatga taactgcaag
    12961 aacagtcgca atggttaagg cagacataat ccatttgacc atgccgtcta aaagtttgta
    13021 gcctccaaag agtagcatag cccaaatgat tgcaacgaga atgagggacc actgagtaat
    13081 gctaagtcca atcattggga aggcactggc gatgatagct gagcacagaa tggcaacacc
    13141 agccgtgttg accatagccg aaaagacatt gaggataaag aaaatccaga gatagagttt
    13201 tcctttttcg gcataacctt caaccaaagt ctttccagta tcagctgtgt attcagcacc
    13261 aaaacggaaa aatggatatt taaagacatt ggctaagatg accaagagaa gtagagacca
    13321 accgtaagaa ccgccagctt gagttgagga tacaatgtgg gaacctccaa cagcggcagt
    13381 tgccattagg attccaggtc ccattgcttt tagcttgctt gcccaggttg attgatttaa
    13441 ggaaatagct tgcgacatga taaatactcc tttttgaatt ttcagaataa tctgtatatg
    13501 taatctatat tctacaaaaa attttggaaa aatgcaagaa tattttgaaa aaagatagaa
    13561 aatttacaaa gaaaatctga
//
LOCUS       DAVDWM010000053        13513 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVDWM010000053 DAVDWM010000000
VERSION     DAVDWM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.053"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1..755
                     /locus_tag="VUW49_001813"
                     /pseudo
     CDS             1..755
                     /locus_tag="VUW49_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(922..1125)
                     /locus_tag="VUW49_001814"
     CDS             complement(922..1125)
                     /locus_tag="VUW49_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981689.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEIIID"
     gene            complement(1118..2200)
                     /locus_tag="VUW49_001815"
     CDS             complement(1118..2200)
                     /locus_tag="VUW49_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET7981690.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(2326..3108)
                     /locus_tag="VUW49_001816"
     CDS             complement(2326..3108)
                     /locus_tag="VUW49_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET7981691.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3108..4451)
                     /locus_tag="VUW49_001817"
     CDS             complement(3108..4451)
                     /locus_tag="VUW49_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7981692.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4550..5866)
                     /locus_tag="VUW49_001818"
     CDS             complement(4550..5866)
                     /locus_tag="VUW49_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET7981693.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNILPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLSDLFAI"
     gene            6060..7286
                     /locus_tag="VUW49_001819"
     CDS             6060..7286
                     /locus_tag="VUW49_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET7981694.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7485..9059
                     /locus_tag="VUW49_001820"
     CDS             7485..9059
                     /locus_tag="VUW49_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7981695.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            9323..10111
                     /gene="codY"
                     /locus_tag="VUW49_001821"
     CDS             9323..10111
                     /gene="codY"
                     /locus_tag="VUW49_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET7981696.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10111..10686
                     /locus_tag="VUW49_001822"
     CDS             10111..10686
                     /locus_tag="VUW49_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET7981697.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10768..10853
                     /locus_tag="VUW49_001823"
     tRNA            10768..10853
                     /locus_tag="VUW49_001823"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10802..10804,aa:Leu,seq:aag)
     gene            11137..12267
                     /gene="ugpC"
                     /locus_tag="VUW49_001824"
     CDS             11137..12267
                     /gene="ugpC"
                     /locus_tag="VUW49_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7981698.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(12389..12559)
                     /locus_tag="VUW49_001825"
     CDS             complement(12389..12559)
                     /locus_tag="VUW49_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981699.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEYKEHHNIDA"
     gene            12767..>13513
                     /locus_tag="VUW49_001826"
     CDS             12767..>13513
                     /locus_tag="VUW49_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7981700.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKS"
BASE COUNT         4024 a         2523 c         2921 g         4045 t
ORIGIN      
        1 agaacacaag ctatcattcg aaatcacttt cttaaatatg atagagccgt ccgatgtcgc
       61 gtcaaaatca ttactatgga tatgtttagc ccttactatg acttagctaa acagcctcgc
      121 tttcaaattt ctaggctcag gctgaaacag tctcccagac tgtttcactc ccgaatgcaa
      181 aaattgttct tgatcgcttt cacattgtac aacatcttag ccgtgctatg agtcgtgtgc
      241 gtgttcaaat catgaatcag tttcatcgaa aatcccatga atacaaggct atcaagcgct
      301 actggaaact cattcaacag gatagtcgta aactcagcga taaacatttt tatcgtccta
      361 cttttcgtat gcacttaacc aataaagaga ttttaaacaa gcttttgagc tattcacaag
      421 acttgaaaca tcactatcag ctctatcaac tcttgctgtt tcacttccag aataaggaac
      481 cagagaaatt tttcggactt attgaggaca atcttaagca ggttcatcct atttttcaga
      541 ctgtctttaa aacctttcta aaggacaaag agaaaatcgt caacgccctt caactaccct
      601 attcaaacgc caaattggaa gcgaccaata atctcatcaa acttatcaag cgcaatgcct
      661 ttggttttcg aaactttgaa aacttcaaaa aacggatttt tatcgctctg aatatcaaaa
      721 aagaaaggac gaaatttgtc ctttctcgag cttagctttt tttcaaccca ctacagttga
      781 caaagagcca taaattaatt gtctaactgt ttgggggcag tacatattca acacctgata
      841 ctatgcgttt tattgtggga agattttcct cattttctag tgaaattgaa ctttttctaa
      901 catcttttga tagatgactt cttaatcaat tataatttcc attgttgttt cttggggttt
      961 catcccttgt ctttgataaa aacggagagc accctcgtta tcattccaga cacttagggt
     1021 tagattattg cagccaattt ctttagcata atagcagaaa aatgacaata aaatagcccc
     1081 tagatatttt gaaaggaaag cataggggct atatagttta atcaaaataa agcaaatctt
     1141 tgctggtgct tgtaaagagg tcgaagccat ccttggtaac aacaccgcag tcttcaatac
     1201 gaacaccgac tttaccaggg atatagatac ctggttcaac agagaagcac atgccttctt
     1261 cgatgaccat gtcgtttcct tccatgatag atgggaattc atggacatcc ataccgatac
     1321 catgcccgag acggtggttg aagtactcac cataaccagc tttttcgatg acctcacggg
     1381 cagcgcggtc cacttcatga gcagtcacac ctggcttgat aaagtcaaga gcagcttgtt
     1441 gggcttcaag agtcaagttg taaatatctt tcttgaattg gtctggtttg ccgacagcga
     1501 ctgtacgagt catatctgac gcatagccat tgaccagaac acccaggtca aagaggagaa
     1561 gagcatcatt ttcaacctta ttagctgctg gaatgccgtg tggattcgca gcattatcac
     1621 cagtcaagac catggtatca aagctcattt cgtaaccttc acgtttcatg gcaaagtcaa
     1681 tttgtgcgat gatatctgtc tcagtcttat caagagaaat attgtcaaaa ccaacatgaa
     1741 cagccttgtc agcataaaga cctgcaacca tcattttttg cacttcatca gctgatttga
     1801 tgaggcgcat gcgttggata cgaggagtga ggttgtcaaa ctcagcagtc tcaaaaactg
     1861 ttttcaaacc atggtatttg gtcaagatga gattgtcaaa ctcaacagcg acacgtttga
     1921 agtcaagttg tggaagagca tgtttgattt tttgccatgg attttcagaa tcgacatagc
     1981 ccactactgg gaaggaaacg gtgctacttg cacgttctac ttcaagagct gggacaaaga
     2041 ggagaggttc ctgatctgct aggacaaaga ggaacatttg gcgttcatgg ggatcactgt
     2101 aaaaaccagt gaggtaattg attgtgacgg ggtcagatac gacagcgacg tctagttttt
     2161 ctgattcaag atatgttagg atttgttgta atttagacat atgctacctt ctttctaccc
     2221 ctctattttg gcaaaaaatg agagaaaatg caagggagaa gggtaaaaga aaagacaaaa
     2281 aactccgaca agataacgga gttctttgat atcattttcc tttgtttaag aaaagtcagc
     2341 cttgcttttg acgtcactgt actcttcagc gatttcttgg ctgagaatgt cctcataggc
     2401 agggagttgg atgtcctgac catatttctt cttgagggca gtagtaacta ggcgataagc
     2461 cagattggca ttacgagaga ggataggccc gtggaagtag gaaccaaaga cattcttata
     2521 atgaacccct tcaccgacct tttcttcgtt gtttccattt ccatagacaa cctgtcccag
     2581 cggtttttgg tcatcagaga ggaaggtacg accttggtga ttttcaaatc catagtaggt
     2641 ttcatcgaaa tcttcattgt gaatcttgat gtcaccgata aaacggttat tggtctggtt
     2701 gagcgtgtag tgtcccatga cccctagccc ttcgatacgt tttcctgaag cttcaacata
     2761 atattgaccc aatagttgga aaccaccgca gatagccaga actacaccgt cgttttggat
     2821 gtagttgtca atgctctctt ttttagcagg taggtcgtct gcaatgatac tttgttcaaa
     2881 gtcttgacca ccaccgaaaa aggcgatgtc gtagtgattt tcatcaaagt catcatggag
     2941 agaaacgatg tcaacggtca catgggctcc cagtttttca gccacatact tgagcatgag
     3001 gatgtttcca ttgtccccgt aggtattcat gagatttccg tagaggtggg caatgttgag
     3061 ctgatagggg taattgccat cttttgagga aagtgaagta taaaccatta gttcatctcc
     3121 tttctaacaa tctgacgact agccagcagt tcacgaaatt ccagcatggc agtataagtt
     3181 gccagaatat aggcatgctt gcagtcttga ttctcaatgg tcttgagaac ttgctccaga
     3241 ttactcgttt cagtgatttt ctcagctgga tagccagtca ctcggaggcg acgagcgatt
     3301 tcagaatgac gaacaccgcc agcgttgatt tcaggaatgt ccatgtcagt gatttgctca
     3361 aagtctgcat cccagatcca gctagtgtca attccatctg catagttggc attaaggagg
     3421 acagataggc taaatggata aggtgctagt ttgatcattt cgatagcttg ggttgcaccg
     3481 actggatttt taatcaagac aagggtacat tccttgtcac cgatatgaaa ggtttcttgg
     3541 cgtccaaaga cagcacggct cttgtcaaat ccctgtttga tgagttggga atctgcgccg
     3601 aggaaacggg cgatggccac agcagctagg gcgttataga tattatagag cccgccgatt
     3661 tggataccgt attcttggcc gtctatgaca aagcgagagc gattgttggt caactcaacc
     3721 agttttgtca aacgatagtc gagatcagga cgtttacatc cacaaccttc acagatatag
     3781 gcacccaagt ttgcataggt attatgctca tatttgagga tgccttggca gtcaggacag
     3841 agaatccctt cggtattgta gtgagccagt tgggctggtc ctttttccaa gtcaaaacca
     3901 aaatactcta tagggtttgg aatagttggc ttgtagaaaa gtggactgtc tccgttaagg
     3961 agaacagtag cagttggaac tttccgaatg gcatccaata tcatgttata ggtagtatag
     4021 atttcaccga aacggtccat ctggtcacgg aagatattag taatgacaaa aagactaggc
     4081 tggatatagt cacagatacg agatagactg gcttcgtcaa tttcgaggac ggcaatattt
     4141 ttcccagttt ttgaagattt ggctgttagg aaggttgttg caatccctgt aatcatgttg
     4201 gcaccgcttg ggttggttag aacttgacca taaacctctt ttaaaatgcc gacagtgagg
     4261 gcagttgtca gggtttttcc atttgttcca gtgacaacga caatctcgta gttcttagct
     4321 aggttttgta aaatatcttt atcaaattga agggcgactt tacctgggag cgtacttcca
     4381 cgtccaagac ggcttaaaac gaagtgggaa gaacgcccag caagaaggcc caaagtagtt
     4441 tttaagttca tgtttctatt atatcacaaa agagggaaaa gacagatttg gtgtttcgta
     4501 gaagtaaaaa cagcccaaag agggctgtca gttaagatga gatttcaact taaatcgcaa
     4561 acaagtcact caagttctca gccaaggttg ggtgagtgaa gatttgtttt gtgaagtaag
     4621 tgtaaggaat cttgttgtcc atagcaacag taatgatgtt gatgatttct tgagaacctt
     4681 ctgagaagat gcttgcacca agaatttctt ttgtttcagt attaacaaca gctttgaaag
     4741 ctccgcgaag gtctccattt acgtgaccac gaggcatggc tgcaacaggg atttctttca
     4801 ctgcgtatgg aagtttcaaa tcagctgctt ggctttcagt caaaccaact tgtgaaagtg
     4861 caggtgtgat gaacatagta tttggcacat tgagacggtc ctcaagtgtg tagctgccat
     4921 ctccagcaag gtagctgtaa acaacacgga agtcatcaag tgaaatgtaa gtaaattgaa
     4981 gaccaccgtt gacatctcca actgcaaaga caccaggaac gtttgtttga cagtgtttat
     5041 ctactttaat agcgccacgt tcagttagtt caatatctgt attttcaagt tgaagtggtt
     5101 ctacatttgg tttgcgtcca gttgcgtaga gaagggcgtc gaaacggtaa gtttcgtctt
     5161 cagttacgac aagcacttgg tcaccgtcgt ttttaatttc agtagtatgg atattttgaa
     5221 gcaattcaat accgtcttct tccatgtatt gtttagcaag agctgcgatg gaaggttctg
     5281 cacgaggtag gaatgtatcc aaggcatcta ggactgtaac cttgcttcct agtttattgt
     5341 aaaggccagc aaattcaaga ccgatatttc cgccaccaag gactccaagt ttttcaggca
     5401 atttatccaa gctttggata cctgttgagt caaagacgtt tttgcttgta gcaagtccag
     5461 ggattggcaa gatgtttgaa acagcaccag tgttgatgac gatagtttca gcagtcaatt
     5521 cttgtttttc atcaccagct tggatttcga tgactttatt tgaaaggaag tgagcttcag
     5581 catcaaagat attgacgcca gtaccagcaa cagtcgtata gtttttaccg ttgaggcgac
     5641 cagtgatcgt gtttttggta gcaatgactt cttcaaaaga caagtctttc tcagcagcaa
     5701 ccagcaaagt tttagttggg atacaaccga tgttgataca agttccaccg tacatagctt
     5761 tgctacgttc aacgagggca acttttttgc cagctgaagc caatttacct gctagtgttt
     5821 taccagcttt accaaatccg ataacgatta aatcataagt taacatttag agttcctcct
     5881 attcgcattt cattctagca caagaaaaaa acttttgcac aattctgcta cggaaaagac
     5941 agaaaaagat aaaaacgctt tccagacaaa aaactagtaa ttttttgaca agcttgataa
     6001 actaaaatcc tatctatttt tatataagat agaataaatg ttcgaaaata ggaggtttta
     6061 tgaaatcgaa tcgttatatt attgcctttg ctggggtcat tttacactta atgctgggtt
     6121 cgacttatgc ttggagtgtt tatcgtaacc ctattattga aaaaacggga tgggatcagg
     6181 cttctgttgc cttcgccttt agtctagcaa tcttttgttt gggcttatcg gctgcattta
     6241 tggggcgttt ggtagaaaaa tttggtccga aagtcatggg aagtctatct gcttttctat
     6301 acgcaggtgg aaatatctta acaggatttg caatagaccg tcaggagctg tggttgttgt
     6361 atctcgctta tggcatttta ggtgggcttg gtttgggagc aggctatatt acccctgtgt
     6421 cgacgattat aaaatggttt cctgataaac gtggtctcgc aacaggttta gcgattatgg
     6481 ggtttggttt tgcttcttta ttgactagtc ccatagcgca acacctcatc gcaggggtag
     6541 ggcttgtaga aactttttat attttaggag caagttactt tattatcatg ctcctagctt
     6601 cacaattcat taagcgtcca aatgagcaag agcttgcaat tttatcttct tcagggaaag
     6661 aaaaaacagc ctctttgacg caaggaatgg ctgcaaatca ggctctaaaa agcaatcggt
     6721 tttatatgct ttggattatt ttctttatca acatagcttg tggtttaggc ttaatttcag
     6781 cggcatcgcc aatggcacag gagatggctg gcttgtctac aagtcatgca gcagtaatgg
     6841 tgggtgtttt ggggattttc aatggatttg gtcgcttgct ctgggcgagt ttgtctgact
     6901 atatcggtcg ccctctaacc tttagtatat tactgcttgt caatcttttc ttttctctct
     6961 cactttggct ctttacagat tccgttttat ttgtagttgc tatgtctatt ttgatgactt
     7021 gctatggagc tggtttttct ttgattccag cttatctcag tgatattttt ggaaccaagg
     7081 aattggccgc tctgcatggt tatattttaa cagcttgggc aatggctggt ttagcgggac
     7141 ctattttatt agcagagact tataaaatgg ctcattcgta cacacaaacc ttgttcgttt
     7201 ttctcatttt atacagtatc gccttggctt tgtcttatta tctaggtcgt tcaatcaaaa
     7261 aagaaagtca aaaaccgctt acatgatttt ctgtagaaat ggggctatct tttgctctgg
     7321 tcttgtgtta tactagatag gttgcaaaga aaacagtact tttcttttgt ggaaaagaag
     7381 caacacgatt ttatatctag ttatatgaaa atacgtcata aaaagaaaag tataaactaa
     7441 gccctatagg gtggcttcgc accaccttta gaaagaagaa taacgtgaaa tttaatgaat
     7501 taaacttgtc tgctgatttg ctagcagaaa ttgaaaaagc tggttttgta gaagctagtc
     7561 ctatccaaga acaaactatt cctttggccc ttgaaggcaa ggatgttatc ggtcaagctc
     7621 agactggtac aggaaaaact gcagcctttg gcttgcctac ccttgaaaaa atccgtacag
     7681 aagaagcgac tatccaagcc ttggtcatcg ctccaactcg tgaactagct gtccaaagtc
     7741 aagaagaact cttccgcttt ggtcgtagta agggagtcaa agtccgttca gtatatggcg
     7801 gatcaagcat tgaaaaacaa attaaggctc ttaaatctgg tgcccatatc gtggtgggaa
     7861 ctccaggtcg cctcttggac ttgattaaac gcaaggcctt gaaattacaa gacattgaaa
     7921 cccttatcct tgacgaagcg gatgaaatgc ttaacatggg cttccttgaa gacatcgaag
     7981 ccattatttc ccgtgtacct gagaaccgtc aaactttgct tttctcagca actatgccag
     8041 atgccatcaa acgtatcggt gttcagttta tgaaagcccc tgaacatgtc aagattgcgg
     8101 ctaaggaatt gacaacagaa ttggttgacc agtactatat ccgtgttaag gaacaagaaa
     8161 aatttgacac catgactcgt ctcatggatg tggcacaacc agaactcgct attgtatttg
     8221 gtcgtaccaa acgccgtgtg gatgaattga ctcgtggttt gaaaattcgt ggcttccgtg
     8281 cagaaggaat tcatggcgac ctagaccaaa acaaacgtct tcgtgtcctt cgtgacttta
     8341 aaaatggcaa tcttgatgtt ttggttgcga cagacgttgc agcgcgtggt ttggatattt
     8401 caggtgtgac ccatgtctac aactacgata ttccacaaga tcctgagagt tatgttcacc
     8461 gtatcggtcg tacaggtcgt gctggtaagt caggtcaatc tattactttt gttgctccaa
     8521 acgaaatggg ttaccttcaa atcattgaaa acttgactaa gaaacgcatg aaaggtctca
     8581 aacctgcaag tgtagaagaa tccttccaat caaaaaaaca ggtagctctc aagaaaatcg
     8641 aacgtgattt tgcagatgaa accattcgtg ccaactttga gaaatttggt aaggatgctc
     8701 gcaaattggc tgctgagttt actccagaag aattggcaat gtatatcttg agtctgacag
     8761 tccaagaccc agatagcctt ccagaagtgg agattgcacg tgaaaaacca ctaccgttta
     8821 aaccatcagg taatggtttc ggtggtaaag ctaagggagg tcgtggaggc cgtcgtgggg
     8881 acgaccgtcg agagcgtgat cgccgtggca atggtcgccg tgatgagttc aaaaaaggaa
     8941 gtcgtggcaa cgatcgtttt gataaggaaa aacgttaccg taaggataat aaaaaaccac
     9001 gcaatacttt aagcgaaaag caaacaggct ttgttattcg taacaaaggc gataaataag
     9061 aaaaagcggt tcaaaatgaa tcgctttttt atataaggag accgaatgtg aaaagacaaa
     9121 taaaattatt atattatctt ttataatgtt atgatacaca aaaataggca acttgtcaat
     9181 agaaaaggaa caatttaatt aaaaatattc tggtttttaa ttaaattgta agtcaatgca
     9241 agtcaataag gaattttcag ataattattg aaaaagagac gtttttatgt tataatgata
     9301 gcgattaaat tcgaggtgaa aaatggcaca tttattagaa aaaactagaa aaattacatc
     9361 aattttgaaa cgctcagagg agcagttgca ggatgagctc ccttacaacg ctattacgcg
     9421 tcagttagcg gatattattc attgcaatgc ctgcattatc aatagtaagg gacgtctgct
     9481 tggctatttt atgcgttata aaacaaatac agatcgcgta gagcaattct tccaaactaa
     9541 gattttccca gatgactacg ttcaaggggc taatatgatt tacgaaacag aagcaaactt
     9601 gcctgttgag catgatatga gtattttccc tgttgagagt agagatgatt ttccagatgg
     9661 cttgacgact attgcaccga ttcatgtatc ggggattcgc cttggttctt tgattatttg
     9721 gcgtaatgat aaaaaattcg aagatgagga cttggttctt gttgagattg ccagtaccgt
     9781 tgttgggatt cagcttctta acttccaacg tgaagaagat gagaaaaata ttcgtcgtcg
     9841 tactgctgtc accatggcgg tcaataccct ttcttactcc gaactccgtg ctgtttcagc
     9901 aattttaggg gaattaaatg gaaatgaagg gaagttgact gcgtcagtga ttgcagatcg
     9961 tatcggaatc actcgctctg tgattgtcaa tgctcttcgt aaacttgagt ctgcggggat
    10021 tattgaaagt cgctcacttg gaatgaaggg aacctatctt aaggtcttga tttcagatat
    10081 ttttgaagaa gtgaagaaaa gagattacta atgacaaagg ctttaatttc gattgattat
    10141 acagaagatt ttgttgctga tagtgggaag ctgacagcag gtgctccagc tcaggcgatt
    10201 tcggatgcca tcagcaaggt aactcgatta gcttttgaac gaggagatta tatcttcttt
    10261 actattgatg ctcatgaaga aaacgattgt ttccatccgg aaagtaagtt atttcctcct
    10321 cataatctga ttgggacgag tggacggaat ttatatggag atttggggat cttttatcaa
    10381 gagcatggtt cagacagtcg tgtcttttgg atggataaac gccattactc agctttttca
    10441 gggactgacc tagatattcg tttgagagag cgtagagtgt ctaccgttat cttaacaggt
    10501 gtcttgacgg atatctgtgt cctacataca gctatagatg cctataatct aggatatgac
    10561 atcgagattg ttaaaccagc tgttgcttcc atctggcctg aaaatcatca atttgcccta
    10621 ggtcatttca aaaatacact cggagctaag ttagtagatg aaaatctaaa tgaactttct
    10681 gagtaacctg gttggtgaaa atgaaaaaat ttgaaaaaaa gtgttgacaa gcatccccaa
    10741 agttgatata ctagtaaagt aatcgacgcg gggatggcgg aattggcaga cgcgcaggac
    10801 taaggatcct gtgaccgctt taggtcgtga gggttcaagt ccctctctcc gcataggaaa
    10861 agagctagct aaagctagct cttttgtatt tctctaaaaa attagaatcc agtcaaatgt
    10921 agtggagaat cagcgaaaat atgggaaaac tgatccttgt ttctgctttt gtctatagaa
    10981 gaataataaa gattatcttc ttcaaattct ccgatattat ctaaagtttt gtgcaagttg
    11041 cacagaactt gtttattttt ttggtcatct tgccatagaa atataaagcg ttttcatata
    11101 taatataatt atcaaaagac aaaaggagtt cacctcatgg tagaattgaa tcttaaaaat
    11161 atttacaaaa aatatccaaa cagcgaacac tattcagttg aagatttcaa cttgaacatc
    11221 aaagataaag aatttatcgt tttcgtagga ccttcaggat gtggtaaatc aactacactc
    11281 cgtatgattg ctggtcttga agacattaca gaaggtactg catctatcga tggcgtggtt
    11341 gtcaacgacg tagctccaaa agaccgtgat atcgccatgg tattccaaaa ctacgctctt
    11401 tacccacaca tgactgttta tgacaacatg gctttcggtt tgaaattgcg taaatacagc
    11461 aaagaagaca ttaacaaacg tgttcaagaa gcagctgaaa tacttggatt gaaagaattc
    11521 ttggaacgta aaccagctga cctttcaggt ggtcaacgtc aacgtgttgc catggggcgt
    11581 gcgattgtcc gtgatgcgaa agtattcttg atggacgaac ctttgtcaaa cttggatgcc
    11641 aaacttcgtg tatcaatgcg tgctgaaatc gctaaaattc accgtcgtat cggagctaca
    11701 actatctatg taactcacga ccaaacagaa gcgatgacac ttgcagaccg tatcgttatt
    11761 atgtcagcta ctaagaaccc tgctggtaca ggtactatcg gacgtgtaga acaaatcggt
    11821 actcctcaag aagtttacaa aaatccagtt aacaaattcg ttgcaggatt catcggaagc
    11881 ccagctatga acttcatcac cgtgaaattg gttggtagcg aaattgtttc tgacggtttc
    11941 cgtttgaaag tgccagaagg agcattgaaa gttcttcgtg aaaaaggcta cgaaggaaaa
    12001 gaattgatct ttggtatccg tccagaagac gtgaatgcag aacctgcttt ccttgaaaca
    12061 ttcccagact gtgttgtaaa agcgactatc tctgtatcag aactgcttgg ttcagaatct
    12121 cacctttact gccaagttgg taaagacgag tttgttgcaa aagttgatgc tcgtgactac
    12181 ttgcaaacag gtgcaacagt tgagcttgga tttgacttga acaaagcaca cttcttcgat
    12241 gtagaaactg aaaaaacaat ctactaaaat aaataaaatt caaagcacta caagaaaaga
    12301 tatctcttta tcaattgtag tggagagata tcagttaatc tagggagaga aacaaaatgc
    12361 ttctctcctt tttgctaggg aagtcatatt atgcatctat attgtgatgc tctttatact
    12421 cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc gtacgtatga ttactgattt
    12481 cgtcagtttt atctgcaacc tcaaagatgt actttgagca gcttacggct agtttcctag
    12541 tttgctcttt gatttccatt gagtattatt tgtgggtacc atctacaagt gaagctatat
    12601 gcgtaaacta cgtgagcaat tgaattcgaa ctagagaggt aataataaat ttatgctata
    12661 gttatggtga cttgtatgct tttgattcta gtttatcaaa taatagatta gaattgtcag
    12721 ataatatcat tttgtgttat aatgaagaaa aaacagaggt gttcaaatgt cagaagcagg
    12781 tcataagttt ttagcaaaat tggggaaaaa acgcttacgt ccaggtggaa agcgtgccac
    12841 agattggtta attgcagaag gaggattttc aaaagaaaag agaatactag aggttgcgtg
    12901 taatagggga actacagcaa ttgagttggc acagcgtttt ggttgcaaga taactgctgt
    12961 tgatatggat gctcaagctt tagaagtggc taaaaaatct gctggaacgg caggtgttgc
    13021 tcatttaatc agttttgaaa gagcaaatgc aatgaaactt ccttatcaag atgctagttt
    13081 tgatattgtt ataaatgaag ctatgctgac tatgcaagcc gatcaagcta agaaaaaatg
    13141 tgtaatggaa tatctaaggg tattaaaacc tggaggtctt ctcttgacac atgatgtgct
    13201 tcttaaggaa gctaaagagt ctatcagaca ggaattatca caagcaattc atgtaaatgt
    13261 aggtccttta actcaagatg gttgggaaca ggtgatgata gaatcaggtt attgtgatgt
    13321 gaaagcattg actggtgaaa tgacattaat gaaattatcg ggtatgattt atgacgaagg
    13381 tttgctagga actttgaaaa tttgtgtaaa tgcttgtaaa aaggagaata gaaagcagtt
    13441 tttaactatg tataaaatgt ttgctaagaa taaacagaaa ttgggcttta ttgcgatggc
    13501 tagttataaa tcg
//
LOCUS       DAVDWM010000054        12771 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVDWM010000054 DAVDWM010000000
VERSION     DAVDWM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.054"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(60..1226)
                     /locus_tag="VUW49_001827"
     CDS             complement(60..1226)
                     /locus_tag="VUW49_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET7981701.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
     gene            complement(2007..2675)
                     /locus_tag="VUW49_001828"
     CDS             complement(2007..2675)
                     /locus_tag="VUW49_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET7981702.1"
                     /translation="MTYSQNYLDDILVRMAYHSSGIEGNTISLPETVSIILESTLPRN
                     GKSIREFYEIENHKQAFSYLLDSLANHQALTVGLVQDFHALLVDRLQHDRGQFKQVQN
                     AIIGAEFQTASPAETPYLMTQWADNTAYRLEHAQNEKEVLEILADTHIQIERIHPFSD
                     GNGRTGRLILMYLAMKYLNAPIIIPKDSRAHYMELLANQNVTGLADLFKKSLDYERIR
                     KKQF"
     gene            complement(2814..3551)
                     /locus_tag="VUW49_001829"
     CDS             complement(2814..3551)
                     /locus_tag="VUW49_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7981703.1"
                     /translation="MDNLAQHVGEKIKFFRKENGWTQSVLAEKMSTSKQTISKYEKGI
                     IKVNQDTIFKLADIFGVSIDSFFPSIIEEIATTSPIQSIYDQLTPPRQEKALTYLKKQ
                     LLEQKNENIVSENIISLDDYRESKTLPVIGVVTAGNGITQDDNLNMEKCFYTDEIPDD
                     YDAIAYVVGNSMEPKIKNGDYLFIKNTPRVDYNTIGIFQVDGANYVKKLRQGYLESLN
                     PECADIQLDESNDIRTIGEVVSIYREN"
     gene            3718..3933
                     /locus_tag="VUW49_001830"
     CDS             3718..3933
                     /locus_tag="VUW49_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981704.1"
                     /translation="MQEKLLILRKKQGISQQELADYLKISVTSYGQKERGEKAFTLDE
                     MFALSTFFELSISEIFLPRKSPKKRHN"
     gene            4030..4215
                     /locus_tag="VUW49_001831"
     CDS             4030..4215
                     /locus_tag="VUW49_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981705.1"
                     /translation="MNFKEFKTWLDDAVSVAEAMALPENKGVLDDLIENTANNLAFIA
                     ELVESRQLIYRKPRHED"
     gene            4675..5127
                     /locus_tag="VUW49_001832"
     CDS             4675..5127
                     /locus_tag="VUW49_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981706.1"
                     /translation="MEETAYENLTRRIDRISAEMREIGEKTGLSRLSLLASQTEFIKE
                     ELSRLLWIELPELNDSHKIEAVSKSTTGIFFSPGIFEMDAMRKAFFKRQAKHFFDNEA
                     DQQAYIEHTEKEYLGATVTLKDILFNSKNGTQKANKSCLIEKFEEAMQ"
     gene            5124..5324
                     /locus_tag="VUW49_001833"
     CDS             5124..5324
                     /locus_tag="VUW49_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981707.1"
                     /translation="MTLDLGKMTQAEFDKQMAEIKERNPNIFQLISDFLDRKVTPEEV
                     DEFLKMERAEQVEYIKNYQARV"
     gene            5326..5541
                     /locus_tag="VUW49_001834"
     CDS             5326..5541
                     /locus_tag="VUW49_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981708.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSDQIERENQQTI
                     KTPSEDLNPEYGRYIQLAGVNVWGGME"
     gene            5538..5831
                     /locus_tag="VUW49_001835"
     CDS             5538..5831
                     /locus_tag="VUW49_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981709.1"
                     /translation="MSYTMNLYPDIEKIPDETYFTKEIFLATCLNNTIRIAVARGVLT
                     SQLLDKETKEVVCKTLDTIIETQKAFLQGVASGQITFLNKSKGEEELDESIRE"
     gene            5812..6153
                     /locus_tag="VUW49_001836"
     CDS             5812..6153
                     /locus_tag="VUW49_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET7981710.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            6146..6421
                     /locus_tag="VUW49_001837"
     CDS             6146..6421
                     /locus_tag="VUW49_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981711.1"
                     /translation="MFSLSRESEHDLTHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGARIIFYLVDDIRRFMGVENG"
     gene            6563..7423
                     /locus_tag="VUW49_001838"
     CDS             6563..7423
                     /locus_tag="VUW49_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HET7981712.1"
                     /translation="MTIYEARGFQSNLVYPFDKMEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKVVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            7503..8957
                     /locus_tag="VUW49_001839"
     CDS             7503..8957
                     /locus_tag="VUW49_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HET7981713.1"
                     /translation="MWELYSRIWELGDQWRKKNSYIVNEGKKNERVEIPRPSVAIVAK
                     ALQEICHFTFIGEGVISDISKLYLYHLDLGHYVSSNDIFRKLLLKYDSRLTSNKFFLE
                     LISYIRTETKMKPPLDDYRYIPVANGVYNIKTHKLEEFSPNFVITSKIQTEYNPCARK
                     PILDGWFDFDRWLEALAVNDKEVVALLWQVINEAINPNRTRKKMVLMVGDGNNGKGTF
                     QALLENLIGRSNISNLKPDQFGKEFYLGALEGKVCNIGDDISNKYLDEVSDLMSVISG
                     DPVQVNKKSLQPVEARFRLLCIFSGNDLPRARNKTMGWYRRLCIIPFRADFNGQKERH
                     EIKDRFIKNKELLEWVLFKVLNMPDFDSFIEPEAVQKMLSKYKNDNDYIKVWVENFYI
                     PNGWHEINHVPMFIARNKLKEFAEDIGIDKPKLGQFSNAVMAELEKTTSYKYSAKNGT
                     VSSKFYDVLDPQGFQRKRFVKGIWGIHLEDEDLG"
     gene            9567..9725
                     /locus_tag="VUW49_001840"
     CDS             9567..9725
                     /locus_tag="VUW49_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HET7981714.1"
                     /translation="MEIQNGFIYFKSYGIIKLLEVPRFGSITLKIQDGEIVSSVESKT
                     TIFKKNTD"
     gene            9809..10408
                     /locus_tag="VUW49_001841"
     CDS             9809..10408
                     /locus_tag="VUW49_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981715.1"
                     /translation="MPTLQEVKNQMDKVRTQLEIFDRFDEEIKKAEQEVKAIKAKKAD
                     LQTFEDFQAINAKEKYIADMKAQRTKLEKERIDSIVADARKINASGYLETALEQDETV
                     KRQRQEIKQKSIELLELIANYNENYKNTAKRLADEVRETGIEELFDRLNTSPEYSGVS
                     KPYIYSGVAGYMGNQHRYLDPSDDLAYFVNRINLFEGEQ"
     gene            10408..10638
                     /locus_tag="VUW49_001842"
     CDS             10408..10638
                     /locus_tag="VUW49_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981716.1"
                     /translation="MKDEYKQLFQEIEEEKRQADLLQGLAKNVESISKSYEEEAVTLE
                     EFKAMGYKRRHELFEKDKALYDKLLGKEKRGL"
     gene            10702..10968
                     /locus_tag="VUW49_001843"
     CDS             10702..10968
                     /locus_tag="VUW49_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981717.1"
                     /translation="MVRSSKARLEKLEEYAGKMEYATTDECISLYVDCLRFAFQQDEI
                     KIDMDDFIKTPFKDIKLADYKKSKKRMDELREMTGISEFSELEQ"
     gene            11019..11360
                     /locus_tag="VUW49_001844"
     CDS             11019..11360
                     /locus_tag="VUW49_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET7981718.1"
                     /translation="MANYLMTGNVKKASELSEITRKTAYNYLNDATFKRIYRERRSEQ
                     LKESTTLLQNASVEAVNVLREIMLDKTVSPYARQQSAQSILNMAYKAVEMVEVVEQLE
                     ILEAKILDEGD"
     gene            11347..11598
                     /locus_tag="VUW49_001845"
     CDS             11347..11598
                     /locus_tag="VUW49_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981719.1"
                     /translation="MKAIKNRLNKLNKLAVVDEVDTVFLTQIDTGHFEVRYRIGGKNP
                     KDIIEVYPTKEEAEKDISEIRNTKGLRIFWDNLALLEDD"
     gene            11954..12372
                     /locus_tag="VUW49_001846"
                     /pseudo
     CDS             11954..12372
                     /locus_tag="VUW49_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
BASE COUNT         4259 a         2125 c         2750 g         3637 t
ORIGIN      
        1 gctaaaattc cttgatatag ctatttccca taactgaaat aataatttta tggacttttt
       61 taggaacttt ttaatttttc aatagctttt tcataaaatg aagtagcatt tttctgattg
      121 tctttggata ggtggctata aatatccatg gtcatagaaa gttttgcatg accgagccgt
      181 gtttgtatct ctttgtaagg tagtccagca ttgagcaaga tactagcatg agtgtggcgg
      241 aaggcgtgga acgtaaaacg agggcatcca gcacgttcca aatgttttac taacctgaaa
      301 ctaagcattc tagcgtctat atgcttatca aagctattag agaatacttt ctcataggtc
      361 aaccccattt ctttccctat ttgggattgt cttgctttat agagccgtag catgagcacc
      421 gtcttattat ccaagtcaat taccctaata cttgatttag tcttagggcc agtgatttct
      481 ttgataatat ttaacgtttt atttacgtct agtgtgccgt tttttaggtc aatatcagac
      541 catttgaggg ctaaacactc acgaatacgc aaaccagtcg ctaaaagcgt cttatatagc
      601 accgtatcgt agaaattttt gtaagtgttt ggcagctgct ccaggtaagt caagaatttt
      661 tttaagttat catcatccag atatttcagt ttttgccctt ctttttcctt acgacgtggc
      721 acgataacat cacgggcagg gttaaagggg atagcttgca aagatacggc atatttaagt
      781 atgcgtttgt ttagagagtg aaggagcgga tattcttgga atcctttgcc tagttgatta
      841 tagtcttttg cccattgatt gacttgcttt tgaatgattg ggggcgtcag tttatccagc
      901 ctataatttc cgaaagctgg aagtagatat ttatctattt tgaatttggt acccattaag
      961 gtcgattttt taaccgtatg gcagtacgtt tctagccaat tctccaccag ttcggcgtaa
     1021 gtctttatct caactgcttt atatactgta gaaccgtctt tttcaaagtc tatttgagcc
     1081 tgtagtgctt tggctttgag ttctctcttg gttcgccctg atatagtcgt cttgactttc
     1141 ttacctgtta cggtatcgat accaaggtag acgctagagc gatagactgt agacccgtct
     1201 tttttggtgt attctgttat tttcatggtt ttactccttt ttccatcagc aggcaagcaa
     1261 ttagaaaaga ttttgagttt ataccatgcg agggactacg agaatgcccc tattttcgat
     1321 tttaagcagt cagacggtaa attgtaccag aatatgaaac aaggcggata tggggcttat
     1381 atgagcttgt ttgtgatggg tgtttttgat ataatgtttt cgccccctca atttagagaa
     1441 gggaggtgtt atatatgcta gagttgtttt ccctttttct agctccgtta cttgttaacg
     1501 tcttatctga gcttttcaag ttatggataa agagacgtag caagtagctt ttaacccttt
     1561 aagagggtag caaaaaaccc catcgctgta acggtggggc tttttagtta catatgctag
     1621 aagcactgtt tttcccttta tgctttcatt ctagcataca agccccaata tttcaagagt
     1681 tttatttttt ttgatattgt ttagagcgct ttaagcgttt ctaaaatgcc aaagtgttga
     1741 aaatcgtcta aattataccg cattatactt cattagagag ttatagactg atgagcgtga
     1801 gaaatgccgt cagtcacttt ttaaatgcaa gagtggtata ataatagcgt agactaagag
     1861 gtttgaaaga gtcccaactc aacagtaacc agacctttct gcttccaatc tctcaagatg
     1921 ctactaatcg cctgaatctt tcccgttatt tcagttcagg cgatattaga caagtcagca
     1981 gcagctagct tgtctttttt gttgttttaa aactgttttt ttcgtattct ttcgtagtcc
     2041 agtgactttt tgaagaggtc agctagtccc gttacatttt ggtttgcgag gagttccata
     2101 taatgcgccc ttgagtcttt tgggatgatg ataggagcgt ttagatactt catagccaag
     2161 tacataagta tcaggcgacc agtacgaccg ttaccatcgc taaatgggtg aatccgttca
     2221 atctggatat gggtgtctgc taaaatctct aatacttctt tttcattctg agcatgctcc
     2281 aaacgataag cagtattatc cgcccattgt gtcatcaagt agggcgtttc tgctgggcta
     2341 gccgtttgaa attccgcccc gataatggca ttttgaactt gcttaaattg tccgcgatca
     2401 tgttgcagcc tatccactaa taaggcgtga aaatcttgaa ccagccccac agtaagagct
     2461 tgatggtttg ccaatgagtc caagagataa gaaaaggcct gtttatggtt ttcaatctca
     2521 taaaactcac gaatactttt gccgttgcgt ggcaaggtgc tttctaagat aatgctgacg
     2581 gtttcgggta gagagatagt attcccttca ataccgctag aatggtaggc catgcgtact
     2641 agtatatcgt ccagatagtt ttgtgagtag gtcataattc tcctatctat ttgtcacttt
     2701 tgcccatttt tgcgctttag tctgtaaatc ggctttctat tcttcaaatt tccccgtttt
     2761 tgcacaatag acccatggga tggggttgct attggcgaat atgggggagt tttttaattc
     2821 tctctatata tactcacgac ttcgccgatg gtgcgtatat cgttgctttc gtctagttga
     2881 atatcagcac attcagggtt aagactttct aaataccctt gacgcagttt cttgacgtag
     2941 ttagcgccgt ctacttggaa gataccgata gtgttatagt caactcgagg tgtattcttg
     3001 ataaataggt agtcgccatt ctttatcttt ggctccatag agttgccgac aacataagcg
     3061 atagcgtcgt agtcgtctgg tatttcatca gtataaaagc atttttccat gtttaggtta
     3121 tcgtcttggg ttatcccgtt tccggcggtt acaactccga taacagggag cgtcttgctt
     3181 tctctgtagt cgtccagact gataatgttt tctgatacta tgttttcgtt tttctgctcc
     3241 agcagctgct ttttaagata agtcaaggct ttttcttgtc ttggtggagt gagttggtca
     3301 tagatggatt ggatggggga agtggtagca atttcttcaa tgatagaagg gaaaaagcta
     3361 tctatagaca cgccgaaaat atcagcaagt ttgaaaattg tgtcttgatt aacctttata
     3421 ataccctttt cgtatttact tattgtttgt ttactggttg acattttttc agctagtaca
     3481 gattgagtcc agccgttttc ttttctaaaa aattttatct tttctccaac gtgttgagcg
     3541 agattgtcca tgttatcccc tcctttcgta ttttcattat aacaaaaaag tcacttaaaa
     3601 agcaactttt tttaattttt aacaaaaaaa ctgttgacaa gtcgcttaaa aagcgataat
     3661 atagttttgt cgcctaataa gcgacttaca aaaaactaaa aaaggagggg aaagttattg
     3721 caggaaaaac tgttgatttt acgcaaaaaa cagggtattt ctcaacaaga attagctgat
     3781 tatttaaaaa tatcagttac ttcttatggg caaaaagaac gcggagaaaa ggcttttaca
     3841 ttggatgaaa tgtttgcgct aagtactttt ttcgagttat cgattagcga aattttttta
     3901 ccaagaaagt cgcctaaaaa gcgacataac tagaaaggag taacccaatc gcaatactat
     3961 attacatcta caagatactc cactggtgct ttaccactgg ggattgataa caaaaaaagg
     4021 agaaaagaaa tgaactttaa agaatttaaa acatggctag atgatgccgt gagtgtggct
     4081 gaagctatgg cattacctga aaacaagggt gtgcttgacg acttaataga aaatacggcc
     4141 aataacctag cttttatcgc tgagttagtg gaaagccgtc aactgattta tagaaaacct
     4201 agacatgaag attaaaaaag acaataaaaa agtcacttgc tgatagttta gcgaccgagg
     4261 caagcgacaa gatttagggt atagatattt tttctatatc tcaattataa caaaaataaa
     4321 ctaatcaatc aataacaact aatagcaggc aagcaattag aaaaggtttt gaaaagcata
     4381 gagcgccaac tcttaaaact ggtactttct cacgctttca atttggcgag tctgagcgtg
     4441 aggctagcaa ttacaagaaa aaaactgtat aagaaacaac tcaaaaagac ccacgctcag
     4501 aaatttggcg accgagagca tgaggcatgc gacaggaaaa actgtataag aaacaaccat
     4561 tcaaaatccc cacactcgcc atcgctcaac tttgagtgtg aggatatcct ggatagtaaa
     4621 aggcattaaa aagcccttag ggcaactaca caacatagaa acagaggtaa aaatatggaa
     4681 gaaacagcat acgagaattt aactagacgt atagacagaa ttagcgcaga aatgcgagaa
     4741 attggcgaaa aaactgggct tagtagactt tcgttattgg caagtcagac agaattcatc
     4801 aaagaagaat tatcccgttt actttggatt gaactccctg aattgaatga cagccataaa
     4861 atcgaagcgg tctctaaaag cactacggga atctttttct cccctggtat ttttgaaatg
     4921 gacgctatgc gaaaagcatt ctttaaacgc caagctaagc acttttttga caacgaagca
     4981 gaccaacagg cgtatataga acatactgaa aaggagtatt taggggctac tgtaacctta
     5041 aaagatattc tttttaactc taaaaatgga actcagaaag caaataaaag ttgtcttata
     5101 gagaagtttg aggaggcaat gcaatgacac tagacctagg caaaatgaca caagcagaat
     5161 ttgataaaca aatggctgaa atcaaggaga gaaatccaaa catttttcaa ctgatttctg
     5221 attttttaga tagaaaggta acacccgaag aggtggatga gtttttgaag atggagcgag
     5281 ctgaacaagt ggaatatatt aagaattatc aagcgagggt ataacatgaa tgagctagat
     5341 ataagtaaca cacaggctgc tgtcttaatc gtgattttga ttggcttact gctttattta
     5401 aaccaccgag accgcaaaaa aagcgaccaa attgagcgag aaaaccaaca gacgataaaa
     5461 acacctagcg aggatttaaa ccctgaatat gggcgatata tccagcttgc aggagtcaac
     5521 gtatggggag ggatggaatg agttacacaa tgaatttata ccccgatatt gaaaaaatac
     5581 cagatgaaac ctattttact aaagagattt ttctagcaac gtgcttgaat aacacaatca
     5641 gaatagcagt agccagagga gtgctaacat ctcaactttt agacaaagaa actaaagaag
     5701 ttgtttgtaa aaccttggat acaatcatag aaactcaaaa agcttttttg caaggggtag
     5761 cgagcggaca aataaccttt ttgaataaaa gtaaaggaga ggaggaactg gatgagtcta
     5821 tcagagaatg acaagcgagt tttaagacta atcaaggtag gggctgagaa ctccataaca
     5881 gggtcagaaa ttagcctgac aaccaagcta acagaaagaa ctgtacgtga cattatcaag
     5941 cgtctagtag ttaagcacaa catccctatt gtaggggtta gatgtggcgt ttttagtggg
     6001 tactttattc cggcgaataa aggcgagtta ctggatggcg ctaaggcttt ttacaaccaa
     6061 gtacaggagg agagcaagcg cctagcggtg ttgatgaata gcgacctaga aagctacaaa
     6121 gaaaccttga aggaggtggg cggatatgtt tagcttaagc cgagaaagtg agcatgattt
     6181 gacgcatggc attctggagg tagtggaaag atacctggaa gcgcgtgaga aagtaccgcc
     6241 aagattgacc aagttaataa atagagttga actcaaagaa gagttaaaaa ttagtgacaa
     6301 cacgctgaat aagtgggaaa gtcagggttt aagacgctat caaccaccgg acgatggtgc
     6361 tagaataata ttttatttag tggatgacat tcgtaggttt atgggggtgg agaatgggta
     6421 gatataacac acacccagcg caaggcggag gtcattatca tgacattaaa ttgtataagc
     6481 accgtggcag atctttggaa cagttccaag aacaaaagct actgaagaag ttaaaaaaaa
     6541 aaacgcaaaa aggggcgata gtatgacaat ttatgaggct agaggtttcc agagtaatct
     6601 tgtctatcca tttgataaga tggagccgtt ccagtacatt gaacgcttta agcctttggt
     6661 agtccctgaa agtgctgatc cggaggaata caaaagaacc caggcgccat attgtttaag
     6721 tggtaaggtt atgccggaga aaaacgggag ttacaaacga aacaactcca gtctaattta
     6781 tcgggatttg attttccttg attatgacga tatacagggg acgactgagg acttcataga
     6841 agccgtttct agcgctttgt ttggctactc ttatatctta tacccgacta tcaaacatag
     6901 catagaaaag ccccgttttc gccttgtggt gaaatctaac aacgtgatga acgaggcaac
     6961 ctataagcag gtagtaaaag agattgctga caagattggg ctaccctttg acatggccag
     7021 tttaacctgg tcacaactcc aagggctacc agtcacaaca ggcgacccag caacttatca
     7081 aaaggttgtg gagcatggcc tagattatcc agtccccaag gttgaaccaa gagcgaagca
     7141 aggaactact gagcgttata aacctagagt aagtggccag aggtcaatga ctatgaggat
     7201 tattgacacg cttttcaacg gatttggaga cgaaggaggg cgcaacgtag cgctgacacg
     7261 ctttgtggga ttactcttca ataagtgggt tgattgtgac ctagagaccg cctatgaact
     7321 tacaaagata gcaaatagcg tgacagttga accattgcct attgaagagc tagaccgcac
     7381 ttttagcagt atagcacggg ctgagtatag aaagagaggt tagaaccata gaactagaag
     7441 aattagcaaa cctaaaaagt gaaatcttgg aggtcagaga gcaagaacaa cctcctagaa
     7501 cgatgtggga actttatagt aggatttggg agcttgggga tcaatggcgt aagaaaaact
     7561 cctatatcgt caatgaagga aagaaaaacg aaagggtaga aatcccacga ccaagtgttg
     7621 caatcgtagc caaagcgtta caggaaattt gccattttac ctttataggt gagggggtta
     7681 taagtgatat tagtaagtta tatctttacc atttggattt aggccattat gtatcaagta
     7741 acgacatttt ccgaaaattg cttttaaaat atgactcacg tttgacttct aacaagttct
     7801 ttttagaact tatctcctat atccgtacag aaactaagat gaaaccaccg ctggacgatt
     7861 atcgatatat tccagtagcg aatggagttt ataatattaa aactcataaa ctagaggaat
     7921 ttagtcctaa ttttgtcatt accagtaaga tacaaacgga atacaatcca tgcgccagaa
     7981 agcctatttt agatggttgg tttgattttg atagatggtt agaggcttta gcagttaacg
     8041 ataaagaagt tgtagcttta ttgtggcaag tcataaatga ggctatcaat cccaaccgaa
     8101 cacgtaagaa aatggtgcta atggttggag atggtaataa tggaaagggt acttttcaag
     8161 ccttgctgga gaatttaata ggacggtcaa atattagtaa tttgaaacct gaccagtttg
     8221 ggaaagagtt ctatttaggc gctttggaag gaaaggtttg taatattgga gatgatattt
     8281 ctaacaagta cctggatgaa gtttctgacc ttatgagtgt aattagtgga gatccagtgc
     8341 aggtcaataa aaaaagcttg caacccgtgg aagcacgttt cagactattg tgtatctttt
     8401 cgggtaacga tttgcctaga gctagaaata agaccatggg gtggtataga cggctttgta
     8461 tcattccttt ccgtgcagat tttaacggtc agaaagaacg tcatgaaatc aaagaccgat
     8521 ttataaagaa caaagagctg ctagagtggg ttttgtttaa agttttaaat atgccagatt
     8581 ttgatagctt tatagaacct gaagcggtac aaaaaatgct tagcaagtac aaaaacgata
     8641 acgattatat caaagtttgg gtagagaatt tctacatacc aaacgggtgg catgaaataa
     8701 accatgttcc aatgtttatt gcaagaaata aattaaaaga gtttgctgaa gatattggga
     8761 tagataaacc taaacttgga caatttagca atgcagtaat ggccgaattg gaaaaaacaa
     8821 cgtcatataa gtactcagcc aaaaacggaa cggttagctc taagttttat gacgtacttg
     8881 acccacaagg ttttcaacgt aaaagatttg ttaaaggtat atggggaata cacttagaag
     8941 atgaagattt aggttaggaa atttaatact aaaaaaccaa ttacaccaag ggttatagca
     9001 atttaggtta tgcaaaaaat agaaacataa cctaaacact aacctttgta aacgttgata
     9061 taaaagtctt tgcgagtatt taggttagta ggttagtttt tatttctaat atctctatat
     9121 aaaaataata tatatacgtg ttatataggc gcgaacttaa aaagtaaaac taaccctaat
     9181 ctaacaccct tgaaacgttg atatagtagg cttttgaagg gttatttttt aaaactaatc
     9241 aaaacctaat ctaatagaat aaaggagcaa aacagatggc gatgaacatt ttgtcaaaga
     9301 gtggctagat tagaagaaac cgttgagcgg atcagtgagg cgctggatgg ctttattgag
     9361 aatgcgaacc ataacgctat agtaagcaat aaaaaacatc gaacggattc aggaaggttt
     9421 tgaaatagtc tttaaagagc ttgatgaact aaaagcagta cctgacaaac actaaaacat
     9481 ttaaacaaaa tggggtagat agtaccgacc cccttgcaat ggtgtaacta ctagtgacac
     9541 ccttaaaaca gatgaaagga taacgaatgg agatacaaaa tggctttatt tatttcaaaa
     9601 gttatggtat aataaaactg ttagaggttc caaggtttgg gagtatcacg cttaaaatac
     9661 aagacggtga gattgtatcc agcgtggaat ctaaaacaac aatatttaaa aagaatactg
     9721 actgaaaaaa tcagaggtat agcataaagt agagtaatct attttggcta tgcctctttt
     9781 gtttttggaa agattggagg aaaaagaaat gccaacattg caagaagtca aaaaccagat
     9841 ggacaaagta cggactcaat tagagatttt tgatcgcttt gatgaggaaa tcaagaaagc
     9901 agagcaagaa gttaaggcta ttaaagctaa gaaagcggac ttacaaactt ttgaagattt
     9961 tcaagctata aacgcaaaag aaaagtatat tgctgatatg aaagcacaaa gaactaaact
    10021 cgaaaaagaa cgaattgact caatcgtggc agacgctaga aaaatcaatg cctcaggtta
    10081 tttagaaaca gcattagagc aagatgaaac agtgaaacgc caacgtcaag aaatcaaaca
    10141 gaaatctatt gagcttctgg agttgattgc aaattataac gaaaactaca aaaatactgc
    10201 taaaagattg gcggatgagg taagagaaac aggtatcgaa gagttatttg accgcctgaa
    10261 tacgtcacca gaatatagtg gagtaagtaa gccttatatt tacagtggtg tagctggtta
    10321 tatgggaaac caacaccgct atttagatcc gagtgatgat ttagcgtatt ttgtgaatcg
    10381 tatcaattta tttgagggag aacaataatg aaagatgaat ataaacaact atttcaagaa
    10441 atcgaagaag agaagagaca agctgactta ctgcaaggac ttgccaagaa cgtagaaagt
    10501 atttcaaaat cttatgaaga ggaagctgtg acccttgaag agtttaaagc tatgggctac
    10561 aagcgtagac atgaactatt tgagaaagat aaagccctgt atgataagct actaggcaag
    10621 gaaaaacgcg gtttgtgatg tggaggggag caatcccctt tttttacata aatcagacag
    10681 aattacacac gaggtagggg aatggttaga agttcaaaag caagactaga aaagttagaa
    10741 gagtatgctg gaaagatgga atatgcaaca actgatgaat gtatatcttt atatgtggat
    10801 tgtttaagat ttgcttttca gcaagatgaa ataaagatag atatggatga ttttattaaa
    10861 acaccattta aagatattaa gctagcagac tataagaaat caaaaaaacg tatggatgaa
    10921 cttagggaaa tgactgggat aagtgagttt tcagaattag agcaataaag cgaggtgaac
    10981 agtatttgac aggattaagc agaaaaaaag aaatatttat ggccaattat ctcatgactg
    11041 ggaatgtaaa aaaagctagt gaattaagtg agattaccag aaagacggct tacaattact
    11101 tgaacgatgc aacctttaag agaatttacc gtgaaagacg tagcgaacaa cttaaggagt
    11161 ctactacgct gttacagaat gcaagcgttg aggcagtcaa tgtattaagg gaaatcatgc
    11221 ttgataagac agtgagcccg tatgcgagac aacaaagcgc ccaaagcatt ctaaacatgg
    11281 cttataaggc ggttgaaatg gttgaggtag tggagcaatt agaaatatta gaggcgaaga
    11341 tactagatga aggcgattaa aaaccgttta aataagttga ataagttagc tgtggttgat
    11401 gaagttgata ccgtgttttt gacgcagatt gatactggac attttgaggt aagatatagg
    11461 ataggtggaa aaaatcccaa agatattata gaagtctatc cgacaaaaga agaagcagaa
    11521 aaagatattt cagaaattag aaatacaaaa ggattgcgga tattctggga caatttggct
    11581 ttattggagg atgattagag cttatcattt atcaagcaga taagagcaat aaaaaccctc
    11641 cttaactccc tgagaaacga caagcagata agcgaagttt ttagccctct tatttcggtt
    11701 ctactccttg cagaatgaca aggaaataag agagataaaa acatccctta attgcttatc
    11761 aaaccacaag gagatacggg ggctatctcc ctccccacgc acttctatga gcttcacacc
    11821 gtcattgtac attttttctc acggggaagc atttgaagct agagcgaaca tagagaaaac
    11881 gaaaaaagcc aaggcactcc gcctcagcta atagttctca caaagactat tatatcatag
    11941 aggaggccga ggcatgacac cagagcaggt aaaagaaaaa ctagagggcg ttaagtggat
    12001 aaacaaagag atagaaggct tatatttaga gcttgcagct ttagaaagtg gtattatcaa
    12061 aaagcaagaa ctaagtaata ccagagtaca aacaagcagg gtaaatacag cagagaataa
    12121 ctttataagt gtttgaagct aaaagaggat acactccaga gaattgagcg ccttaccgag
    12181 gaaagaatgg caatatctga gctaatcgat aagctggcca atccgcttga gcgttctgtt
    12241 ctaagacttt tttatttgaa tgatcttgta gctttggaag ttgctgagga aatagataaa
    12301 tctaccacct cagtatatcg aataaagaag gaagctatag aacacttggc aggtatggta
    12361 aatggggatt gatttaacct tgccacagaa ttaggagcgt atggggagct taagccccat
    12421 atttgctccg atttcgcttt gtttggggtt ggacgattaa aacaccatgg ataaactaga
    12481 agcccttaga aacgaatcag ggggactttt gtgaggtcat aatcttataa accacgcgct
    12541 tcatggtata atatacctat cagcaaccag caataaaaaa agcacgtttg aacgtgctag
    12601 tttcttgcct gctgaactca tcgatttagt agcccttttg tggggctttt tttgtggact
    12661 ttttgagaat ttttaagaac ttttaatgat ttctaatcaa atgagatttt tcaaaaagtc
    12721 agtaatatca agggtttagg cgctataaac aagtttaaaa gcgtatagat t
//
LOCUS       DAVDWM010000055        12481 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVDWM010000055 DAVDWM010000000
VERSION     DAVDWM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.055"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(313..945)
                     /locus_tag="VUW49_001847"
     CDS             complement(313..945)
                     /locus_tag="VUW49_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981720.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(947..1714)
                     /locus_tag="VUW49_001848"
     CDS             complement(947..1714)
                     /locus_tag="VUW49_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HET7981721.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            complement(1765..3504)
                     /locus_tag="VUW49_001849"
     CDS             complement(1765..3504)
                     /locus_tag="VUW49_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7981722.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSIISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            complement(3528..5636)
                     /locus_tag="VUW49_001850"
     CDS             complement(3528..5636)
                     /locus_tag="VUW49_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HET7981723.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFF
                     VTALLYVIQSLVPIANRYIIDTNFKDDSYSSRMLFTILFIFTVSFSLMYLLRQIYVAS
                     LKYIMDKEISYDFMKHLIYLPYSFYEKRTLGDILFRANSIVYIREILSNNFIAAILDL
                     LMIVVYAVVLFSFSKYMVIFLISLSLALSIVMYPIIKISKNLIDKNIKEKVNVQNITS
                     EVISKNSDIKLTGEEEFWINKWDNFNTKQLIIGRKLDIHLSIVSSITNVLQIILPVLT
                     LIVGVNIKTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGYFLRIEDVFNT
                     KSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGETVAIVGESGSG
                     KSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTF
                     GHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKP
                     SVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEI
                     GTHRELLELGGIYKQLYSNN"
     gene            complement(5633..6562)
                     /locus_tag="VUW49_001851"
     CDS             complement(5633..6562)
                     /locus_tag="VUW49_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET7981724.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            complement(6543..7247)
                     /locus_tag="VUW49_001852"
     CDS             complement(6543..7247)
                     /locus_tag="VUW49_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7981725.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            complement(7597..10587)
                     /gene="lanM"
                     /locus_tag="VUW49_001853"
     CDS             complement(7597..10587)
                     /gene="lanM"
                     /locus_tag="VUW49_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HET7981726.1"
                     /translation="MKYKIKNVHKTLFIKERYQILSERNDNSLNNFSLNNWRSTMSII
                     SEKTFEEMLKLNNYNKKIFSNTVNYDYTEEQIRLYSEYAKNSEWYKFAEGSFEIFRKH
                     PQRINFKKDFSRILEPFIINLLKLINNYLENKEYEWIDKNGNIFSSLVFYLEDLIYPW
                     IVKPLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENILNFYNKYPVLLRQISESC
                     LRFYTYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKI
                     VYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKKLP
                     EYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDT
                     LFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKI
                     DFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTR
                     VLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDI
                     PIFYTDTNINEIYDDFGNHFISLHQFSGYDYFMKYLPNNNLENLEKQLDIIEIYFPSF
                     DQRKKGYKLNLSNRKKLPNESLVTILHNEVENIADSLVINNIYDEYPIWSIPVNFKGS
                     KWCLEQTGNDMYRGRAGILLFMYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGL
                     TGDIGYFIVLSIIEDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINT
                     LVRYYRRGIEKERVLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKV
                     TRLKKYKELILQLVPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGS
                     MAIINFYIDYMKEMDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIA
                     YTFLRYIALNQNDKKIVIPSLLEI"
     gene            complement(10704..10892)
                     /gene="pneA2"
                     /locus_tag="VUW49_001854"
     CDS             complement(10704..10892)
                     /gene="pneA2"
                     /locus_tag="VUW49_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HET7981727.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(10899..11123)
                     /gene="pneA1"
                     /locus_tag="VUW49_001855"
     CDS             complement(10899..11123)
                     /gene="pneA1"
                     /locus_tag="VUW49_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HET7981728.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKICISAAVSGIGGLVSYNNDCLG"
     gene            11588..12451
                     /locus_tag="VUW49_001856"
     CDS             11588..12451
                     /locus_tag="VUW49_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981729.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
BASE COUNT         4228 a         2189 c         1596 g         4468 t
ORIGIN      
        1 tagttttctt ttaaaacggt tgtaggagga aaatctctcc tacactatta ttgattctga
       61 gagaaaattg caaaatatca tctaccaaac tttcaattat taagattttt tgcagtttat
      121 ctgaattaga atcgcaaggc ttctcaataa tataattgtc gaattttttc attctaactt
      181 cctctccatt atttcttcct ttatcaagaa aaagttacct caatattaat aaagtacaat
      241 tctaaattaa ctccaattct tgattcatga taatatttca gtaactcatc gtgcaattaa
      301 aaacaaggat ttttatttca gatgatccat cgtgaacact tcatcagcca tctcccaaat
      361 tgccggatta tgtgttgcga taatgattag cctattatca tctcgaagag atagcaaaat
      421 ctccataatc aactgagagg ttgctgggtc tattgaagct gttggctcat ctgccagaat
      481 aaagggtgga ttctttaaga taatttttgc caaggcaacc cgttgcgatt ctccgcccga
      541 taactcaaag atgcgcttat ctaggtcaag ataagccagg ccgacctgtt ctaaagcctg
      601 cttctgcctc aaccgctgtt ccgaccgact caacttttga ccaatgagac ctagcttaag
      661 gttttcttca atactttggt tttcaattaa gccaaagttc tggaagaggt agcccaattc
      721 gtgacggaaa aaatcactcg atttataatt ggccaagtct ttacctcggt aaaaaatcgt
      781 cccatcataa ggttctaatt tcccaatcat gttcatcaag gttgtttttc cgctaccact
      841 tgaaccaatt aaggcataga cttttccagc ctcaaatgtc attgaaagat tcgaaaataa
      901 ctctcgttct ccaaaagatt tactcacctg tttaagttca atcatattaa cctcccttca
      961 aaacaagaat ggacatcttg ttttctttct gcatttggac atgtaactgt aaaagagata
     1021 gaccagtaaa gagtaacaag actaagaaag caactcctat ctctacctga agaaatacac
     1081 tcgcaacaaa tcccagtaaa aacacaccca gttgagcaaa gagataagtg cgatggattt
     1141 ctaagaacct gagacctgca atgcgtttga taaaaatggc acgtctaaat tcttcaaagt
     1201 agagcctatt catagtgtta aacaacaaga ttgaagttgc aatcccaagc acagctcctg
     1261 ctagcattac ccaacgttcc ctctggatat tatccaataa tgtgatgtag ttgtggtaac
     1321 ctgtttgcat ttctgagacc caattttcaa tgccttgtct ctggataagc tcctgggcat
     1381 cagacaattg attaaagaga acgtagttct gtactgcgtc tatccaaaac aaaatggact
     1441 gtggaccagt tgattggggt gttataacaa tgatgattgg atctttcaaa aactgctggt
     1501 aagaaatagg ggtcgtatta tacacaaaac gatcctgacc tgattctaaa taacctaccg
     1561 tagcagtcat ttgctgtcgt tcatcttgac tagatatgcg actggttaag tcgtcttcaa
     1621 aaacagattt ataatgttct tcctctgaac ggaggtgttc aggcagcaat aagacaaact
     1681 cccctacatc aaggtgattc atcttttgac tcattttagt tgaactgtag gacttacttt
     1741 ttcagagggc tccaaatttt attttcattt acttttttta gcccataaaa tatttaccat
     1801 acttataaaa agtagtaata ctccaactaa actgacttgt aatgaattat atgtattttc
     1861 tgaagtgtta ggtaaataat tttgagagta cccttctcgg gcaatagtaa taatattgtg
     1921 tgtgtttatt tgtttaattc caatagtagt ttgaattctc tcttccaact caattaaatt
     1981 aacattatct atgaaactat cccaactttg ttcattttta tattctaatt gtaacatatt
     2041 caaatcatat ctcaatacgt ttggtatatt taacactttt ataccatttt ttactggtaa
     2101 tgtttcttcc agaatttttt ttacaaacat ataattatag ggctgatctt ttaatttgta
     2161 tttatcaata atcattgcaa tcgcaccaga aactttagga gcagcaaatg agtttccata
     2221 aagatacgta tatcctccta gtgttgccga aaaaatccaa tcttctttat atttttctgc
     2281 attaataaag ttattaagtc ctaattctga aagagataat gttgagcctg caggcgcata
     2341 tattgcatca gaatcccctt tattgctaaa atctgataaa ttacctaagc gatctatgcc
     2401 tccgaccgaa attatagatg agaaataact tggaacatca actactaatc ctggttttct
     2461 tacttttttg cgtgaactaa taagtttcaa taaatctgac tgatttgata catttaggga
     2521 gtcattccct aatgcagcta ctataattac tcctttttga ttcgcgtaat caatagcctt
     2581 tttatacttc aaaaatgttt caaaatcatt tgttccgtcc tcatattctc catcaatcat
     2641 taaatattgt ccagtactaa gattgataat atcattgcca tcatctacag catcaaaaat
     2701 tgcttttaca atccaatctg gactagctga cgacttacca aatattctat agacgttaat
     2761 attaacgtga ggatttactc cattaatatt gtcatcccct acaatttgag ctacaacagc
     2821 cgtaccatga cctaatctat cttgaatatc tgatattatt ccttcctctc cctcctcttt
     2881 tcctaaatat cctttattgg ggacatagtt ttttgagtga tttgaaagat ttttcagtaa
     2941 tccagtattt tgtaaatcga ctcctgaatc taaaatagca attgaaactt tagaattact
     3001 tttagaatat aatttataac tttcgcctcc accagtaact ttatctatat cccattcata
     3061 ttttcttaca atcgacgatt ctaagttatc tttgtcatgc tctatatttc ctcctctatt
     3121 atctagatga gaaacatttt ccttaatttt attatctaaa ttatcttcgc ctagtaaatt
     3181 attactttca gagttaacaa tatcgttatt tatttgtggt tcaagttcca atttacaaca
     3241 gtttttttct aaatgtttat ctgttgttac aaataattct gtcttatcga tttgaatacg
     3301 aaatttattt tggtctactt cttcttgatt tttattagaa tagtcaacct gtttttcatc
     3361 agattcagaa aaactttcgc tagtctgagt gtgagttata ttgccatcaa gatttttagg
     3421 tatttcttga gcagtaacag aattattgaa caaacaaaag aataatatcg ctaaagtcca
     3481 atattttttt ttcataatct tcataaattt acccttttaa tcaaaaacta attatttgaa
     3541 tacaattgct tataaatgcc tcctagttca agaagttctc tatgggtccc aatctctaca
     3601 atttcaccat tatgcattac taagatctta tctgcagact tgatagtatc tagtcgatgg
     3661 gcaattaaaa tcttagtggt accgatttca cttaagtaag aagttgtaat ttgttgagat
     3721 aaattatcta aactacttgt tgcttcgtcc aaaacaacta ccgaaggttt cgacaaaaga
     3781 caacgagcta aaagtatcat ttgctgctgc cctccagaaa agttattacc ttcttcagcg
     3841 atgattgtct ctaatccaag aggtaaagat ttaactacat tgctaagacc aattgaattt
     3901 agatttgtca ttaattcttc atctgaaaca aagtgtccaa atgtcaaatt ctctcttaag
     3961 gtaccataac ttagggttga attttgtaac actgctccaa aaatctttct atacaatgtt
     4021 tgaccaattt cttctttttc tactccatca acttctattg aaccaatatt aggttctaat
     4081 aaacctaata aaattttagc taatgtactc ttacctgaac ctgattctcc aacaatagca
     4141 acagtttctc cttttttaat agtaacattt attcctttca aaacataatc atcaaataat
     4201 ccatatttat accaaatatc ttttaattct atttttttat caaattttat atcttgactg
     4261 actctttctg gaattaattc ggatttagta ttaaacacat cctctattct taaaaaatat
     4321 ccctttaata acattaattg tatatagtta tcacttaaag aaattatagg agaaataaag
     4381 tatggtgaga ctgtacttat tgctacaatt tgtcctaacg tcaattgttc gaatgttttt
     4441 atatttacac ctacaataag ggtcaaaaca gggagaataa tttgtaaaac attcgttata
     4501 ctactaacaa ttgataaatg tatatcaagt tttcgaccta tgatgagctg ttttgtatta
     4561 aaattatccc atttgttaat ccaaaattcc tcttctccag ttagcttaat atcactattt
     4621 ttagaaatta cttcggaagt aatattttga acattaacct tttcttttat atttttatca
     4681 attaaatttt ttgagatttt tatgattgga tacattacaa tagatagagc tagacttagt
     4741 gatattaaaa agattaccat gtacttagaa aagctaaata aaaccacagc ataaaccaca
     4801 atcattaaca aatcaagtat agctgctata aaattatttg atagtatttc tcttatataa
     4861 acaatagagt tagctctaaa aagtatatcc cctaaagtac gtttttcata aaaactgtaa
     4921 ggtaaatata tcaaatgttt cataaaatca tagctaatct ctttatccat tatatatttt
     4981 aaggatgcaa catatatctg tcttaataaa tacattagtg agaatgaaac agtaaatata
     5041 aataatatag taaataacat tctagacgaa tacgaatcgt ccttgaaatt cgtgtcaatt
     5101 atgtatctat tagctatagg tactaatgat tgtattacat acaataatgc tgttacaaaa
     5161 aataaaatcc ctagcttatt cctatactta aaaaaatact ttaaaacagg cgacttctta
     5221 ttatctttac gagacataaa gctatctaac tttttaaatg ttaaaatgat atttgaataa
     5281 tgcctttcaa attcacttat tgacattctc tgcctgcctt ttgcaggatc taaaatataa
     5341 aacttgtttt tactaatttt ttcaagaata ataaaatgag aactctccca gaagcaaatg
     5401 agtgggaaac tgagcggttt taaatctctt accgaagctt taaaagcatg agaacttata
     5461 ttaaaatctc ctaaaacttt acttatagat aggagagaat atccagatct ggatgatggg
     5521 tagatttcac gtagttcatt taaagaaatc tcatatttaa aatagttcaa tatcatggca
     5581 cagcatgcta atccacattc tgaattttcc atttgatata tcattggaat tttcacaata
     5641 cttcctcctt gttcaaaata aagtcgataa tttcttcttg tctaaaatct actaatttct
     5701 ttagatactt aatcttttct aaatatttat tatttttcga gtaaatatag tttgacaagg
     5761 taaaaacaac ttcttctaaa gataagttct gtggaataat ccaatcagaa gatttatgta
     5821 gtaatttata gtactcatgg ataatatttt cctgaatatt tgaatcaaaa acttcatgat
     5881 tagcaattgt aaatgctgac ccttcacctt tcacttcaaa gtaaaggttt atcaaacatt
     5941 tttcaaattt tgaaaaaaaa gatttttctt tagaggttaa ttttgcaaaa tcgtcgattg
     6001 ttatataagg attattatag agactgttcg caaagaatct ctgatatgtt tttgaatctt
     6061 ttgaatacaa aactatctct ctaatagcat tgccatctgt tccatcaatt ggtaaacata
     6121 ccgtaactag aaaaagaatt atattcaaaa taaaaaattc tgatgcgtac ggcacaaatc
     6181 ccaaaagtgc taatattgcg acaattaggt tagctccacc tcccccaaag aagtagaaca
     6241 ccaaattcct atcactattt ttttcattag taatgtttct attactcatt tgacaataac
     6301 cgaatgctaa taacactgga aatttgaaat atattttttt tctgaaatag aagaaaaagg
     6361 gagtagcaag catctctagt ttataagata aacatcttcc cactaaaaaa tgacctagtt
     6421 catgtaatgt aattgatatt aacgaaatta aaatcaatcg aaaataatag attaatgaat
     6481 catttggaaa aattatcaat aataggaaca ataacggaat caaacataaa tatatgacag
     6541 agttatttaa tattttcaac ataataccat tcctctaaac tattagcttc aaaaaggcgt
     6601 tttttctccc aatacatctt ctcaaaatgt tcggaatcat aattttctaa aattaatttt
     6661 aatgtctggt aagctctttc ttgataatcc gttgttttgt acttaatttt cccttcaagt
     6721 acatcttcaa ttttataagt tgcctccatc aactgagcct ctgcaatatc tttgagtgaa
     6781 ttggtaattg aaacttggtg taatatctgt cctcccatat atgaaaatat atctctaaga
     6841 tattctgaca cattatcaga gccgttactc tcagcaacat ctaatgttac aacaaacttt
     6901 ccagctaatc gaaaaagatg gctccacccc ccaatccttt caataaagtt ttttgtgtcc
     6961 acagatacgt tttgtaaata tacaggagaa gagataatta taatatcaga ctctaataac
     7021 tcttttttta taacacctcc atcatcagca ttactttgcc tatcaattcc tttcttaaac
     7081 aactcttctg aatcagaatt agatatttct agctctgaat tgaaaggtgt cctgaaagat
     7141 atatcaacat tatttctact agaaatgata cttgaaagtc tcttagtata ctctaaagtc
     7201 ttagagttat gatttcgcac tcctgcatat ataaatattt tattcatttt aattcatcct
     7261 ctcaatttga atttagtaga tttttcaaga tagtatggta caaaaacaga cttttgttga
     7321 ctcacattat tacatatgtt ttgtattaaa ccaaaatcaa tactattttt ggagtaattt
     7381 tgattttagt ttaaaatcat ttctataaca gtagcatata cctcaagccg tttagcaatt
     7441 agaatagaac ctttctttat tatattatta tcttaacgaa aagctacact attaaaaata
     7501 ttttatagaa ttacatatta aactagtcaa tcttggtatt tttatattgc ttaatgagtg
     7561 gacacctcta ttttagaaac aaaactataa attaagctag atttcaagta atgaggggat
     7621 aactatcttt ttgtcattct gattcagtgc gatatacctt aaaaaagtat aagcaatacc
     7681 agtcacacct gtatacaaag aaaaatctgg gaaattgctt gtttggacga tacgatactc
     7741 tccttctttt gatttattca ttacaacact acacaataaa gactccaatt ccatactagt
     7801 atccatttct ttcatgtagt cgatgtaaaa atttattatg gccatacttc catggcaaaa
     7861 tgtatcatta tctaaactag ctacaattcc ctctggaaca ctttggggat gattaactaa
     7921 tgtcccaaat tctccactac accacttcaa agaatgaatt ttgattttct ccctaggaac
     7981 tagttgtaaa attaattctt tatatttttt aagtcttgtc actttataaa tattttttaa
     8041 tgtaaaaatt acacctgata gtccatggcc aaaactatat ccaaaattac tattatctct
     8101 ctcgcttaca tcattatata gcgtatcacc taaacttaat actagcctta gaacacgttc
     8161 cttctctatt cctctcctat aatatcttac cagtgtatta attaaaggta gaagaccatt
     8221 aatatagtca gacttgtttg aaacacttgc aaaatcagtc ttttcaagct cagttaaaac
     8281 actctttata taatttaagc atgcgagagt atttgtatcg taatcctcta taatggatag
     8341 aacaatgaaa tatcctatat ccccagttaa accaaatgtg gtcttagata aagaaacaga
     8401 tggcggaatt gcagataaca ttttattgta cagttgagta tatgatgatt tatctttcaa
     8461 taattttaca tagtacataa acagtaatat tccagctcta cccctataca tatcattacc
     8521 cgtttgttca agacaccatt tagaaccttt aaaattaaca ggtatactcc aaattggata
     8581 ttcgtcataa atattattaa taaccaaaga gtctgcaata ttttctactt cattatgcag
     8641 aatagtaact aaactttcat ttgggagttt ttttctatta gataagttta atttatatcc
     8701 tttttttcgc tgatcaaagc ttggaaaata aatttcaatg atatcaagtt gcttttctaa
     8761 attttccaaa ttattattag gtaaatattt cataaaatag tcatatccag aaaattgatg
     8821 tagggaaata aaatgatttc caaaatcatc gtagatttca ttgatatttg tatctgtata
     8881 aaaaatcgga atatctaata acctcatttg ttcacattcg cttgctacaa taccttgatt
     8941 agaaaactta ttgctccaga gattttccaa tgctttttct ctatctaaca tttcttcata
     9001 aaaatcagga tgatataaaa aagatagtac tgaagcatag ctatttgtgt ctctaaaaag
     9061 tacccttgtc tttaaaccat acaagtttgc ttttaatagc attttaaatt cttctgtttt
     9121 atttaactct tcaaatatca gataaaaatc cctaaaacct tttttgaaat cttttatata
     9181 cttatcaaat tctatatcac catcccgaac aggcaggttt ttcccacctt caaaatcaat
     9241 tttcccaata tcaaacttta ccttatcagt atttaaatta attaaaactt gaccattaaa
     9301 agcatccttc ttaaaatttc ctgtaagagc actcatctca acatcaagac catcctccct
     9361 ttttactccc tctgttggaa gaagcaaagt tcgagtcacc ctgtgaaaca gggtatccag
     9421 caaactctga ccagttttat catcaaatag aggttgctgt aacattgttt caaagtctat
     9481 tattacaggc atatctccat aggaaattaa attttcaaaa tgtatatcac tcccattaaa
     9541 taaatatata aatgctaaaa gcacaccaaa attataataa tattcaggta attttttctt
     9601 ttgctctaga ggtagataat caataaattc ttcatagctg tgatccgaat aagcaatagt
     9661 tcgaggtatt cttatcctaa ttttttcatc aacctttcgg atatactcag caatagtatt
     9721 tagtgagtta ttgattatta aatttttagg cttgtaaaca attttcgcgt catcaaagaa
     9781 gagtatcaaa acagtttttc cttggctgtg tgtatcaccc tttccaaatt ttatatcatt
     9841 taatttcccc cttagcccta attcttcttc tagcacacta aaatcatttt ctaaatttga
     9901 taaaatttct ataaaataag tatagaaccg aagacaagac tccgatattt gcctcagtaa
     9961 aacgggatat ttgttataaa aatttaatat attctcttcc ttgtcaaaca atgttataaa
    10021 atatttgtac cgttgctgct ccgattcccc ttcaagtaaa cctttttcac gcaatgaatt
    10081 tatctctaaa accaaaggtt taacaatcca aggatagatt aaatcttcta aataaaatac
    10141 tagagaggaa aaaatatttc cattcttgtc aatccactcg tattctttat tctctagata
    10201 attattgatt aatttaagca aattaataat aaagggttcc aaaattctag aaaaatcttt
    10261 cttaaaatta attctttggg gatgttttct aaatatttca aatgaaccct ccgcaaattt
    10321 ataccattcc gaatttttgg catattctga atatagtctt atctgttcct cggtataatc
    10381 atagttcaca gtgttagaaa atattttttt attgtaatta tttaatttca acatttcttc
    10441 aaaagttttt tcagaaataa ttgacattgt agaacgccaa ttatttaatg aaaagttatt
    10501 taaggaatta tcatttcgtt cagataatat ttgatatctt tccttaataa acaatgtttt
    10561 atgcacattt ttaattttat atttcattta cttacctcaa acctatacga cttactattg
    10621 ttttaaaaaa aaaacctctc tcaaaattat tagttacgag agaggaaaat aaagagcaac
    10681 ttttattttt aaaattgaat attttatttt ttcttgttgt ctttgccaca acgagtctga
    10741 gctgacccca aatatgatgc aataaaactt aatgtagcag aacatataat tgttgatcta
    10801 gggtcagcac catctgtgcc accataaacc aattgcattt cttcaagaga taattctttc
    10861 aaactctttc cgattacaaa atcatttttc attttaattt accccaaaca atcattattg
    10921 tagctaacta gccccccaat accagaaaca gctgcactaa tgcatatttt cgagctagct
    10981 gcagctgatt tcaaaataat aggagtaggc gtccaacgtg gctctgcacc atcagatgca
    11041 ccataaatta aactcatttc atcagcagac aatgatttta aactttttcc acaaaatttt
    11101 tcgtttgaat taaagttggt cataagtaat aactcctcct tataaaaagt taaataagtt
    11161 gctctatatt tttcgttttt tcagataaat tataattctc tcattctcgt gcaaaatatc
    11221 gggttttaat ccgccttccc aacatcaaga ccattggact tcttttgcga ttgcactcga
    11281 atctccaatt gtttattctt ctgaataaac tgttggtaag gaattgtatt actaaacata
    11341 aaaacaccta gcaatgcaaa tgtaactaat tttttaattc cacgtttttt tctcatgcaa
    11401 tgaaacccct ttctttttcc ttgtttttat tctaacagat atattttatt tagtcaagta
    11461 tttttatgta atttttaact tttttttttg tttattacca taaagaatta tttattcctt
    11521 taacttgcta ttttgaacta aaaactttat aatgaaatta agcaaatagg aaggtttatt
    11581 atctttaatg aatacactcg cagagaaatt cagattgaaa agaaaagagt tgagactctc
    11641 ccaacaaact cttgcagaag gaatttgtga acaaagtcag attagtaaaa ttgagagagg
    11701 gcatttcatt ccctccgcag accttttgtt caaactctct caacgacttg aagtaccatt
    11761 agattatttt tttaatgaac aaattgaaat taaatctaac ctctctaatt tcaagcaatt
    11821 gtctgctcga ctattagacg atagaaatta tgacgatttg gaatatattt atagaataga
    11881 gattgaacga agtacttttc taacactaga agaccgaact taccttgaat ggattaaagc
    11941 tattattgac ttctatcaat atgacagtaa gtgtgaggca atttcttcat tggaaaatat
    12001 attattaaaa gtctcttcaa atactctgat ttatttaaag gcgttgaata ctttatctaa
    12061 tttctattcc ttagtgggtc gtgaacaaga atatgaggca aactactctc atttaataga
    12121 gttgtatcag acaaaaaatt tggaccatca agagttttta tttggctaca tcagagttcg
    12181 ttacaactac gctcactacc tagtatcaaa ggaaaaatat aatgaagcca tccaagaagc
    12241 tcttgaaacg attgaactct gtaaacaaag acagacaagc taccaattgg ctcctctact
    12301 tattcttgta ggaaatgctg gagccaaatt tctagacaga gaacaagtca aaaattatta
    12361 tatagaagca agagagttat gtaagattta taacaatcct ttaatgttga tgaagataga
    12421 aaattatttg aaggaactag atactgttta gttaatcttg acattatagt ttttctgaaa
    12481 c
//
LOCUS       DAVDWM010000056        11489 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVDWM010000056 DAVDWM010000000
VERSION     DAVDWM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.056"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            39..1154
                     /gene="ychF"
                     /locus_tag="VUW49_001857"
     CDS             39..1154
                     /gene="ychF"
                     /locus_tag="VUW49_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7981730.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            1225..1794
                     /locus_tag="VUW49_001858"
     CDS             1225..1794
                     /locus_tag="VUW49_001858"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7981731.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            1795..5304
                     /gene="mfd"
                     /locus_tag="VUW49_001859"
     CDS             1795..5304
                     /gene="mfd"
                     /locus_tag="VUW49_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7981732.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            5373..5639
                     /locus_tag="VUW49_001860"
     CDS             5373..5639
                     /locus_tag="VUW49_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET7981733.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            5632..6000
                     /locus_tag="VUW49_001861"
     CDS             5632..6000
                     /locus_tag="VUW49_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7981734.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            6120..7388
                     /locus_tag="VUW49_001862"
     CDS             6120..7388
                     /locus_tag="VUW49_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET7981735.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            7385..8662
                     /gene="tilS"
                     /locus_tag="VUW49_001863"
     CDS             7385..8662
                     /gene="tilS"
                     /locus_tag="VUW49_001863"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7981736.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            8666..9208
                     /gene="hpt"
                     /locus_tag="VUW49_001864"
     CDS             8666..9208
                     /gene="hpt"
                     /locus_tag="VUW49_001864"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7981737.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            9224..11182
                     /gene="ftsH"
                     /locus_tag="VUW49_001865"
     CDS             9224..11182
                     /gene="ftsH"
                     /locus_tag="VUW49_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7981738.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(11246..>11489)
                     /locus_tag="VUW49_001866"
     CDS             complement(11246..>11489)
                     /locus_tag="VUW49_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981739.1"
                     /translation="LKQVHPLFQTVFKTFLKDKEKIVNALQLPYSNAKLEATNNLIKL
                     IKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA"
BASE COUNT         3935 a         1703 c         2388 g         3463 t
ORIGIN      
        1 aatattaggg attgaaatga aaacggagaa tgagaaatat ggctttgaca gcaggtatcg
       61 ttggtttgcc aaacgttggt aaatcaacac tatttaatgc aattacaaaa gcaggagcag
      121 aggcagcaaa ctacccattt gcgactattg atccaaatgt tggaatggtg gaagttccag
      181 atgaacgcct acaaaaacta actgaaatga taactcctaa aaagacagtt cccacaacat
      241 ttgaatttac ggatattgca gggattgtaa aaggagcttc aaaaggagaa gggctaggga
      301 ataaattctt ggccaatatt cgtgaagtag atgcgattgt tcacgtagtt cgtgcttttg
      361 atgatgaaaa tgtgatgcgc gagcaaggac gtgaagacgc ctttgtagat ccacttgcag
      421 atattgatac aattaatctg gaattaattc ttgctgactt agaatcagtg aacaaacgat
      481 atgcgcgtgt agaaaagatg gcacgtacgc aaaaagataa agaatcagta gcagaattca
      541 atgttcttca aaagattaaa ccagtcctag aagacgggaa atcagctcgt accattgaat
      601 ttacagatga ggaacaaaag gttgtcaaag gtcttttcct tttgacgact aaaccagttc
      661 tttatgtagc taatgtggac gaggatgtgg tttcagaacc tgactctatc gactatgtca
      721 aacaaattcg tgaatttgca gcgacagaaa atgctgaagt agtcgttatt tctgcgcgtg
      781 ctgaggaaga aatttctgaa ttggatgatg aagataaaaa agagtttctt gaagccattg
      841 gtttgacaga atcaggtgta gataagttga cgcgtgcagc ttaccacttg cttggattgg
      901 gaacttactt cacagctggt gaaaaagaag ttcgcgcttg gactttcaaa cgtggtatga
      961 aggctcctca agcagctggt attatccact cagactttga aaaaggcttt attcgtgcag
     1021 taaccatgtc atatgaagat ctagtgaaat acggatctga aaaggccgta aaagaagctg
     1081 gacgcttgcg tgaagaagga aaagaatata tcgttcaaga tggcgatatc atggaattcc
     1141 gctttaatgt ctaaaaatta ataaatggtg tcaattaggt tggaaaaaaa ttccaaccct
     1201 tttggctttt gaaaggaaaa ataaatgacc aaattacttg taggcttggg aaatccaggg
     1261 gataaatatt ttgaaacaaa acacaatgtt ggttttatgt tgattgatca actagcgaag
     1321 aaacagaatg tcacttttac acacgataag atatttcaag ctgacctagc atcctttttc
     1381 ctaaatggag aaaaaattta tctggttaaa ccaacgacct ttatgaatga aagtggaaaa
     1441 gcagttcatg ctttattaac ttactatggt ttggatattg acgatttact tatcatttac
     1501 gatgatcttg acatggaagt tgggaaaatt cgtttaagag caaaaggctc agcaggtggt
     1561 cataatggta tcaagtctat tattcaacat ataggaactc aggtctttaa ccgtgttaag
     1621 attggaattg gaagacctaa aaatggtatg tcagttgttc atcatgtttt gagtaagttt
     1681 gacagggatg attatatcgg tattttacag tctgttgaca aagttgacga ttctgtaaaa
     1741 tactatttac aagagaaaaa ttttgagaaa acaatgcaga ggtataacgg ataaatggtg
     1801 accttattag atttattctc agaaaatgat cagattaaaa aatggcatca aaatttaaca
     1861 gataagaaaa gacaactaat acttggttta tcaacatcta ctaaggctct tgcaattgca
     1921 agcagtttag aaaaagaaga taggattgtg ttattgacgt caacttatgg agaagcagaa
     1981 ggacttgtta gtgatcttat ttctatcttg ggtgaggaac tcgtctatcc atttttggta
     2041 gatgatgctc ctatggtgga gtttttgatg tcttcacagg aaaaaattat ttcacgggtt
     2101 gaagccttgc gttttttgac tgattcatct aagaaaggga ttttagtttg taatatcgca
     2161 gcaagtcgat tgattttacc gtctcccaat gcattcaaag atagtattgt aaaaatctca
     2221 gttggtgaag aatatgatca acacgcgttt atccatcagt taaaggaaaa tggctatcga
     2281 aaagttactc aagtacaaac tcagggcgaa tttagtcttc gaggagatat tttagatatt
     2341 tttgaaatat cccagttaga accttgtcga attgagtttt ttggtgatga aattgatggt
     2401 atcaggtcat ttgaagtaga aacacaatta tcgaaagaaa ataagacaga actcactatc
     2461 tttccagcta gtgatatgct tttgagagaa aaggattatc aacgaggaca gtcagcttta
     2521 gaaaaacaaa tttcaaaaac tttatcacct attttgaaat catacctaga agaaattctt
     2581 tcaagttttc accaaaaaca aagtcatgca gactctcgga agtttttatc tttgtgctat
     2641 gataagacat ggactgtctt tgattatatt gaaaaagata ctccaatatt ctttgatgat
     2701 tatcaaaaat tgatgaatca gtatgaagtc tttgaaagag acttagcgca gtactttaca
     2761 gaagaattac agaatagtaa agcattttct gatatgcagt atttttctga tattgaacaa
     2821 atctataaaa aacaaagtcc agtgaccttt ttctctaatc ttcaaaaggg tttaggaaat
     2881 ctcaaatttg acaaaattta tcaattcaat caatatccta tgcaggaatt tttcaatcag
     2941 ttttcttttc taaaagaaga aattgaacga tataaaaaaa tggattacac cattattctg
     3001 cagtctagca attcaatggg aagtaaaaca ttggaggata tgttagagga atatcagatt
     3061 aaattggatt ctagagataa gacaagtatc tgtaaagaat ctgtaaactt aatagagggt
     3121 aatctcagac atggttttca ttttgtagat gaaaagattt tattgataac tgaacatgag
     3181 atttttcaaa agaaattaaa gcgtcgtttt cgaagacaac atgtttcaaa tgcagagaga
     3241 ttaaaagatt acaatgaact tgaaaaaggg gactatgttg tccatcatat ccatgggatt
     3301 ggtcaatatc taggaattga aaccattgaa atcaagggaa ttcatcgcga ttatgtcagt
     3361 gtccaatacc aaaatggtga tcaaatttct atccccgtgg aacagattca tctactgtcc
     3421 aaatatattt caagtgatgg taaagctcca aaactcaata aattaaatga cggtcatttt
     3481 aaaaaggcca agcaaaaggt taagaaccag gtagaggata tagctgatga tttaatcaaa
     3541 ctctactctg aacgtagtca gttgaagggt tttgctttct cagctgatga tgatgatcaa
     3601 gatgcctttg atgatgcttt cccttatgtt gaaacggatg atcaacttcg tagtattgag
     3661 gaaatcaaga gggatatgca ggcttctcag ccaatggatc gacttttagt tggggatgtt
     3721 ggttttggaa agactgaagt tgctatgcgt gcagccttta aagcagtcaa tgatcacaaa
     3781 caggttgtca ttctagttcc gacgacggtt ttagcgcaac agcactatac gaattttaag
     3841 gaacgattcc aaaattttgc agttaatgtt gatgtgttga gtcgctttag aagtaaaaaa
     3901 gagcagactg caacacttga aaaattgaaa aacggtcaag tcgatatttt gattggaaca
     3961 catcgtgttt tgtcaaaaga tgttgtgttt gctgatttgg gcttgatgat tattgatgag
     4021 gaacagcgat ttggtgtcaa gcataaggaa actttgaaag aactgaagaa acaagtggat
     4081 gtcctaacct tgaccgctac gccaatccct cgtaccctcc atatgtctat gctgggaatc
     4141 agagatttgt ctgttattga aactccgccg actaatcgct atcctgttca gacctatgtt
     4201 ttggaaaaga atgatagtgt cattcgtgat gctgtcttgc gtgaaatgga gcgtggaggt
     4261 caagtttact atctttacaa caaagttgac acaattgttc agaaggtttc agaattacag
     4321 gagttgattc cggaggcttc gattggatat gttcatggtc gaatgagtga agtccagttg
     4381 gaaaatactc tattagactt tattgaggga caatacgata tcttggtgac gactactatt
     4441 attgagacag gggtggacat tccaaatgct aatactttat ttattgaaaa tgcggaccat
     4501 atgggcttgt caaccttata tcagttaaga ggaagagtcg gtcgtagtaa tcgtattgct
     4561 tatgcttatc tcatgtatcg tccagaaaaa tcaatcagtg aagtctctga aaagagatta
     4621 gaagcgatta aaggatttac agaattgggc tctggcttta agattgcaat gcgagatctt
     4681 tcgattcgtg gagcaggaaa tcttttagga aaatcccagt ctggtttcat tgattctgtt
     4741 ggttttgaat tgtattcgca gttattagag gaagctattg ctaaacgaaa cggtaatgct
     4801 aacgctaaca caagaaccaa agggaatgct gagttgattt tgcaaattga tgcctatctt
     4861 cctgatactt atatttctga tcaacgacat aagattgaaa tttacaagaa aattcgtcaa
     4921 attgacaacc gtgtcaatta tgaagagtta caagaggagt tgatagaccg ttttggagaa
     4981 tacccagatg tagtagccta tcttttagag attggtttgg tcaaatcata cttggacaag
     5041 gtctttgttc aacgtgtgga aagaaaagat aataaaatta caattcaatt tgaaaaagtc
     5101 actcaacgac tgtttttagc tcaagattat tttaaagctt tatccgtaac gaacttaaaa
     5161 gcaggcatcg ctgagaataa gggattaatg gagcttgtat ttgatgtcca aaataagaaa
     5221 gattatgaaa ttttagaagg tctgctgatt tttggagaaa gtttattaga gataaaagag
     5281 tttaaggaag aaaattccat ttgatatttt tcttctatta aaatagataa aaatagataa
     5341 aaatggtaca ataataaatt gaggtaataa ggatgagatt agataaatat ttaaaagtat
     5401 cgcgaattat caagcgtcgt acagtcgcaa aggaagtagc agataaaggt agaatcaagg
     5461 ttaatggaat cttggccaaa agttcaacgg acttgaaagt taatgaccaa gttgaaattc
     5521 gctttggcaa taagttgctg cttgtaaaag tactagagat gaaagatagt acaaaaaaag
     5581 aagatgcagc aggaatgtat gaaattatca gtgaaacacg ggtagaagaa aatgtctaaa
     5641 aatattgtac aattgaataa ttcttttatt caaaatgaat accaacgtcg tcgctacctg
     5701 atgaaagaac gacaaaaacg gaatcgtttt atgggagggg tattgatttt gattatgcta
     5761 ttatttatct tgccaacttt taatttagcg cagagttatc agcaattact ccaaagacgt
     5821 cagcaattag cagacttgca aactcagtat caaactttga gtgatgaaaa ggataaggag
     5881 acagcatttg ctaccaagtt gaaagatgaa gattatgctg ctaaatatac acgagcgaag
     5941 tactattatt ctaagtcgag ggaaaaagtt tatacgattc ctgacttgct tcaaaggtga
     6001 taaaatggaa aatttattag acgtaataga gcaatttttg agtttgtcag atgaaaagct
     6061 ggaagaattg gctgataaaa atcaattatt gcgtttacaa gaagaaaagg aaaggaagaa
     6121 tgcgtaaatt cttaattatt ttgttgctac caagtttttt gaccatttca aaagtcgtta
     6181 gcacagaaaa agaagtcgtc tatacttcga aagaaattta ttacctttca caatctgact
     6241 ttggtattta ttttagagaa aaattaagtt ctcccatggt ttatggagag gttcctgttt
     6301 atgcgaatga agatttagta gtggaatctg ggaaattgac tcccaaaaca agttttcaaa
     6361 taaccgagtg gcgcttaaat aaacaaggaa ttccagtatt taagctatca aatcatcaat
     6421 ttatagctgc ggacaaacga tttttatatg atcaatcaga ggtaactcca acaataaaaa
     6481 aagtatggtt agaatctgac tttaaactgt acaatagtcc ttatgattta aaagaagtga
     6541 aatcatcctt atcagcttat tcgcaagtat caatcgacaa gaccatgttt gtagaaggaa
     6601 gagaatttct acatattgat caggctggat gggtagctaa agaatcaact tctgaagaag
     6661 ataatcggat gagtaaagtt caagaaatgt tatctgaaaa atatcagaaa gattctttct
     6721 ctatttatgt taagcaactg actactggaa aagaagctgg tatcaatcaa gatgaaaaga
     6781 tgtatgcagc cagcgttttg aaactctcct atctctatta tacgcaagaa aaaataaatg
     6841 agggtcttta tcagttagat acgactgtaa aatacgtatc tgcagtcaat gattttccag
     6901 gttcttataa accagaggga agtggtagtc ttcctaaaaa agaagataat aaagaatatt
     6961 ctttaaagga tttaattacg aaagtatcaa aagaatctga taatgtagct cataatctat
     7021 tgggatatta catttcaaac caatctgatg ccacattcaa atccaagatg tctgccatta
     7081 tgggagatga ttgggatcca aaagaaaaat tgatttcttc taagatggcc gggaagttta
     7141 tggaagctat ttataatcaa aatggatttg tgctagagtc tttgactaaa acagattttg
     7201 atagtcagcg aattgccaaa ggtgtttctg ttaaagtagc tcataaaatt ggagatgcgg
     7261 atgaatttaa gcatgatacg ggtgttgtct atgcagattc tccatttatt ctttctattt
     7321 tcactaagaa ttctgattat gatacgattt ctaagatagc caaggatgtt tatgaggttc
     7381 taaaatgagg gaaccagatt ttttaaatca ttttctcaag aagggatatt tcaaaaagca
     7441 tgctaaggcg gttctagctc tttctggtgg attagattct atgtttctat ttaaggtatt
     7501 gtctacttat caaaaagagt tagagattga attgattctg gctcatgtga atcataagca
     7561 gagaattgaa tcagattggg aagaaaagga attaaggaag ttggctgctg aagcagagct
     7621 tcctatttat atcagcaatt tttcaggaga attttcagaa gcgcgtgcac gaaattttcg
     7681 ttatgatttt tttcaagagg tcatgaaaaa gacaggtgcg acagctttag tcactgccca
     7741 ccatgctgat gatcaggtgg aaacgatttt gatgcgcttg attcgaggaa ctcgcttgcg
     7801 ctatctatca ggaattaagg agaagcaagt agtcggagag atagaaatca ttcgtccctt
     7861 cttgcatttt cagaaaaaag actttccatc aatttttcac tttgaagata catcaaatca
     7921 ggagaatcat tattttcgaa atcgtattcg aaactcttac ttaccagaat tggaaaaaga
     7981 aaatcctcga tttagggatg caatcttagg cattggcaat gaaattttag attatgattt
     8041 ggcaatagct gaattatcta acaatattaa tgtggaagat ttacagcagt tattttctta
     8101 ctctgagtct acacaaagag ttttacttca aacttatctg aatcgttttc cagatttgaa
     8161 tcttacaaaa gctcagtttg ctgaagttca gcagatttta aaatctaaaa gccagtatcg
     8221 tcatccgatt aaaaatggct atgaattgat aaaagagtac caacagtttc agatttgtaa
     8281 aatcagtccg caggctgatg aaaaggaaga tgaacttgtg ttacactatc aaaatcaggt
     8341 agcttatcaa ggatatttat tttcctttgg acttccatta gaaggtgaat caattcaaca
     8401 aatacctgtt tcacgtgaaa catccataca cattcgtcat cgaaaaacag gagatgtttt
     8461 gattcaaaat gggcatagaa aaaaactcag acgtttgttt attgatttga aaatccctat
     8521 ggaaaagaga aactctgctc ttattattga gcaatttggt gaaattgtct caattttggg
     8581 aattgcgacc aataatttga gtaaaaaaac gaaaaatgat ataatgaaca ctgtacttta
     8641 tatagaaaaa atagataggt aaaaaatgtt agaaaacgat attaaaaaag tcctcgtttc
     8701 acacgatgaa attacagaag cagctaaaaa actaggtgct caattaacta aagattatgc
     8761 aggaaaaaat ccaatcttag ttgggatttt aaaaggatct attcctttta tggctgaatt
     8821 ggtcaaacat attgatacac atattgaaat ggacttcatg atggtttcta gctaccatgg
     8881 tggaacagca agtagtggtg ttatcaatat taaacaagat gtgactcaag atatcaaagg
     8941 aagacatgtt ctatttgtag aagatatcat tgatacaggt caaactttga agaatttgcg
     9001 agatatgttt aaagcaagag aagcagcttc tgttaaaatt gcaaccttgt tggataaacc
     9061 agaaggacgt gttgtagaaa ttgaggcaga ctatacctgc tttactatcc caaatgagtt
     9121 tgtagtaggt tatggtttag actacaaaga aaattatcgt aatcttcctt atattggagt
     9181 attgaaagag gaagtgtatt caaattagaa agaataatct ttaatgaaaa aacaaaataa
     9241 tggtttaatt aaaaatcctt ttctatggtt attatttatc tttttccttg tgacaggatt
     9301 ccagtatttc tattctggga ataactcagg aggaagtcag caaatcaact atactgagtt
     9361 ggtacaagaa attaccgatg gtaatgtaaa agaattaact taccaaccaa atggtagtgt
     9421 tatcgaagtt tctggtgtct ataaaaatcc taaaacaagt aaagaaggaa caggtattca
     9481 gtttttcacg ccatctgtta ctaaggtaga gaaatttacc agcactattc ttcctgcaga
     9541 tactaccgta tcagaattgc aaaaacttgc tactgaccat aaagcagaag taactgttaa
     9601 gcatgaaagt tcaagtggta tatggattaa tctactcgta tccattgtgc catttggaat
     9661 tctattcttc ttcctattct ctatgatggg aaatatggga ggaggcaatg gccgtaatcc
     9721 aatgagtttt ggacgtagta aggctaaagc agcaaataaa gaagatatta aagtaagatt
     9781 ttcagatgtt gctggagctg aagaagaaaa acaagaacta gttgaagttg ttgagttctt
     9841 aaaagatcca aaacgattca caaaacttgg agcccgtatt ccagcaggtg ttcttttgga
     9901 gggacctccg gggacaggta aaactttgct tgctaaggca gtcgctggag aagcaggtgt
     9961 tccattcttt agtatctcag gttctgactt tgtagaaatg tttgtcggag ttggagctag
    10021 tcgtgttcgc tctctttttg aggatgccaa aaaagcagca ccagctatca tctttatcga
    10081 tgaaattgat gctgttggac gtcaacgtgg agtcggtctc ggcggaggta atgacgaacg
    10141 tgaacaaacc ttgaaccaac ttttgattga gatggatggt tttgagggaa atgaagggat
    10201 tatcgtcatc gctgcgacaa accgttcaga tgtacttgat cctgcccttt tgcgtccagg
    10261 acgttttgat agaaaagtat tggttggccg tcctgatgtt aaaggtcgtg aagcaatctt
    10321 gaaagttcac gctaagaaca agcctttagc agaagatgtt gatttgaaat tagtggctca
    10381 acaaactcca ggctttgttg gtgctgattt agagaatgtc ttgaatgaag cagctttagt
    10441 tgctgctcgt cgcaataaat cgataattga tgcttcagat attgatgaag cagaagatag
    10501 agttattgct ggaccttcta agaaagataa gacagtttca caaaaagaac gagaattggt
    10561 tgcttaccat gaggcaggac ataccattgt tggtctagtc ttgtcgaatg ctcgcgttgt
    10621 ccataaggtt acaattgtac cacgtggccg tgcaggcgga tacatgattg cacttcctaa
    10681 agaggatcaa atgcttctat ctaaagaaga tatgaaagag caattggctg gcttaatggg
    10741 tggacgtgta gctgaagaaa ttatctttaa tgtccaaact acaggagctt caaacgactt
    10801 tgaacaagcg acacaaatgg cacgtgcaat ggttacagag tacggtatga gtgaaaaact
    10861 tggcccagta caatatgaag gaaaccatgc tatgcttggt gcacagagtc ctcaaaaatc
    10921 aatttcagaa caaacagctt atgaaattga tgaagaggtt cgttcattat taaatgaggc
    10981 acgaaataaa gctgctgaaa ttattcagtc aaatcgtgaa actcacaagt taattgcaga
    11041 agcattattg aaatacgaaa cattggatag tacacaaatt aaagctcttt acgaaacagg
    11101 aaagatgcct gaagcagtag aagaggaatc tcatgcacta tcctatgatg aagtaaagtc
    11161 aaaaatgaat gacgaaaaat aaccctgaga gaggctggag cctctctttt ttggctcttt
    11221 gtcaactgta gtgggttgaa gtcagctaag cttgagaaag gacaaatttc gtcctttctt
    11281 ttttgatgtt cagagcgata aaaatccgtt ttttgaagtt ttcaaagttt cgaaaaccaa
    11341 aggcattgcg cttgataagt ttgatgagat tattggtcgc ttccaatttg gcgtttgaat
    11401 agggtagttg aagggcgttg acgattttct ctttgtcctt tagaaaggtt ttaaagacag
    11461 tctgaaaaag aggatgaacc tgcttaaga
//
LOCUS       DAVDWM010000057        10847 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVDWM010000057 DAVDWM010000000
VERSION     DAVDWM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.057"
                     /strain="416"
                     /db_xref="taxon:1313"
     misc_binding    71..288
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            401..1405
                     /locus_tag="VUW49_001867"
     CDS             401..1405
                     /locus_tag="VUW49_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7981740.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            1474..2340
                     /locus_tag="VUW49_001868"
     CDS             1474..2340
                     /locus_tag="VUW49_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981741.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            2337..3095
                     /locus_tag="VUW49_001869"
     CDS             2337..3095
                     /locus_tag="VUW49_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981742.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            3185..4945
                     /gene="dnaG"
                     /locus_tag="VUW49_001870"
     CDS             3185..4945
                     /gene="dnaG"
                     /locus_tag="VUW49_001870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7981743.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     ANLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            4948..6057
                     /gene="rpoD"
                     /locus_tag="VUW49_001871"
     CDS             4948..6057
                     /gene="rpoD"
                     /locus_tag="VUW49_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET7981744.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSNLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            6071..6400
                     /locus_tag="VUW49_001872"
     CDS             6071..6400
                     /locus_tag="VUW49_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7981745.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            6515..7558
                     /gene="cpoA"
                     /locus_tag="VUW49_001873"
     CDS             6515..7558
                     /gene="cpoA"
                     /locus_tag="VUW49_001873"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET7981746.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            7574..8899
                     /locus_tag="VUW49_001874"
     CDS             7574..8899
                     /locus_tag="VUW49_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7981747.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            8930..9106
                     /locus_tag="VUW49_001875"
     CDS             8930..9106
                     /locus_tag="VUW49_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7981748.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            9165..9299
                     /locus_tag="VUW49_001876"
     CDS             9165..9299
                     /locus_tag="VUW49_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET7981749.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            9363..10673
                     /gene="obgE"
                     /locus_tag="VUW49_001877"
     CDS             9363..10673
                     /gene="obgE"
                     /locus_tag="VUW49_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7981750.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            10683..10841
                     /locus_tag="VUW49_001878"
     CDS             10683..10841
                     /locus_tag="VUW49_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981751.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         3323 a         1909 c         2514 g         3101 t
ORIGIN      
        1 gcgaatgcta aaagtaaata tcaaaaaaat tctaatagac tattgacaag tagtttaaaa
       61 atgatataat ttaaccattc agaaaagtaa tcatacaaac tttttagaga gtctgtggta
      121 gctgaaaaca gataagtggc gatgatgaaa attgggctga atgctattta gaatttgaaa
      181 ttataaaaat tcggtaagca caccttacag tgcatctcgt tattgcgaga ctgagcgata
      241 gggaaattcc ctataattga ggtggtaccg cgcatcgacg tcctcacaca agttttttgt
      301 gtgaggattt ttttgatgga ggttagtatg gaaagaaaac gatggcgtcg cttgtttaga
      361 taagtgaaat atgttaaagg aaataaaaag gagaaacaga atgaaaaata aacgtttaat
      421 tggaattatt gctgcattag cagtcttagt agcaggaagc ttgatttatt cttcaatgaa
      481 taaatcagaa gctcagaata ataaggatga gaagaaaata accaagattg gtgtgcttca
      541 atttgtgagc catccatccc ttgatttgat ttataaaggg atccaagatg gacttgcaga
      601 agaaggatat aaagatgatc aagttaaaat tgattttatg aactcagaag gtgatcaaag
      661 taaggttgcg acaatgagta aacaattggt tgcaaatggg aatgaccttg tggttggtat
      721 cgcaacacca gcagcccaag ggttggctag tgcaacaaaa gacctaccag ttatcatggc
      781 cgctattaca gacccaattg gtgctaactt ggttaaagat ttgaaaaaac caggtggcaa
      841 cgttacaggg gtatctgacc acaatccagc tcaacaacaa attgaactca tcaaggctct
      901 gacaccgaat gtgaaaacaa tcggagctct ttactcaagt agcgaagaca attcaaaaac
      961 acaggtcgaa gaatttaagg cttatgctga aaaagcaggt ctgacagtgg aaacatttgc
     1021 agttccttca acaaatgaaa ttgcctcaac tgtcactgtt atgactagca aggtagatgc
     1081 tatttgggtt ccaattgata acaccattgc atcaggattt ccaacggttg tctctagcaa
     1141 tcaaagttct aagaaaccaa tttatcccag tgcgacagct atggtagaag taggtggttt
     1201 ggcatcagtt gtaattgacc aacatgacct tggtgtggca acaggtaaaa tgattgtgca
     1261 agtcttgaaa ggtgcaaaac cagccgatac cccagtcaat gtcttttcaa ctggtaagtc
     1321 agtcatcaat aaaaaaatag cacaagaact aggtattact attcctgagt ctgttctcaa
     1381 agaagcagga caagtcatcg aataaataag gtagggagga ggagactctt cccatttttt
     1441 aaaaagatga atatagaaag gattaaataa aatatgattg tttccattat ttctcaagga
     1501 tttgtctggg ctattctagg tctgggaatc tttatgacat ttaggatttt aaactttcca
     1561 gatatgacga cagaaggttc cttccctctt gggggagctg ttgctgtcac tttgataacc
     1621 aaaggcgtga acccattttt agcgacactt gttgctgtag gagcaggttg tttggctgga
     1681 atggcagcag gccttcttta tacaaaaggg aagatcccaa ccttgctctc agggattttg
     1741 gtgatgactt cttgtcactc aatcatgctc ttgattatgg gacgtgcgaa tttaggcctg
     1801 cttggaacca agcaaattca ggatgttttg ccttttgatt cggatttgaa tcaactcttg
     1861 acaggtctca tctttgtgag tattgttatt gctctcatgc tctttttctt ggacactaaa
     1921 ctcggacaag cctatattgc tacaggagat aatcctgata tggctagaag tttcgggatt
     1981 catactggac gcatggagct catgggcttg gtcttatcaa atggtgtgat tgcccttgca
     2041 ggtgccctca ttgctcagca agaaggctat gccgatgtgt ctcgagggat cggggttatc
     2101 gttgtggggc ttgcaagttt gattattgga gaagttattt tcaagagttt gagcttggca
     2161 gagcgtttgg ttactatcgt tgtaggttct atcgcttatc aatttttagt gtgggcagtt
     2221 atcgcacttg gctttaatac aagttacctt cgtttataca gtgccttgat tttagcagtc
     2281 tgcctcatga ttccaacatt taagcaaaca atcttgaaag gagccaagtt aagcaaatga
     2341 cagcaattgt agaattaaaa aatgcaacca aaatcgttaa aaatggcttt gatgaagaaa
     2401 agattatttt aaatgatgtt tccttagaaa tttttgaaca ggactttatc acgattttgg
     2461 gtggaaatgg tgctggtaaa tcaaccctct ttaacactat agcagggacc ttatcactaa
     2521 ctagtggaac tatccgtatt ttaggtgaag atctcactaa gttttcaccc gagaagcgtg
     2581 ccaagtacct gtctcgtgtc ttccaagatc caaagatggg gacagctccc cgtatgacgg
     2641 tcgctgaaaa tcttttaatc gccaagtttc gtggtgaaaa gcgtggattg ttaccacgac
     2701 gcttgactag ctataaggat gaatttcagg caaccattga aaaagtagga aatggtcttg
     2761 agaaacactt gaatacaccg attgagttct tatcaggtgg acaaagacag gctttgagtc
     2821 tcttgatggc aaccttgaag cgacctgaat tactcctgtt agatgagcat actgctgccc
     2881 tggatccaaa gactagtgtt gctttgatgg aattgacaga tgaatttgtt aagaaagatc
     2941 agctaacagc ccttatgatt actcatcata tggaagatgc tctcaaatac ggcaatcgct
     3001 tgattgtcat gaaagaagga cggattatcc aagatttaaa ccaagaagaa aaagcaaaaa
     3061 tgaaaatctc tgattattat caactctttg aataaagtag ggaaaatgag tgaaatggtt
     3121 tacttttttt ctgaaataaa gtatactata taaagtaaac tatgataaca tggaggtatt
     3181 gtgtatggtt gacaaacaag tcattgaaga aatcaaaaac aatgccaaca ttgtggaagt
     3241 cataggagat gtgatttctt tacaaaaggc aggacggaac tatctagggc tctgtccttt
     3301 tcatggcgaa aaaacaccat ctttcaacgt tgtagaggat aagcagtttt atcactgttt
     3361 cggttgtggt cgctcaggtg atgtctttaa gttcatcgag gagtaccaag gggttccctt
     3421 tatagaggca gtccaaatct taggtcagcg tgttggaatt gaggttgaaa aaccgcttta
     3481 tagtgagcag aagccagcct cacctcacca agctctttat gatatgcacg aagatgcagc
     3541 caaattttac catgctattc tcatgacaac gactatgggc gaagaggcca gaaattacct
     3601 ttatcagcgg ggtttgacag atgaagtgct taaacatttt tggattggtt tagcacctcc
     3661 agaacgaaac tatctctatc aacgtttgtc tgatcagtat cgtgaagagg atttactgga
     3721 ttcaggcctg ttttatctct cggatgccaa tcaatttgta gacacctttc acaatcgcat
     3781 tatgtttccc ctgacaaatg accaaggaaa agtcattgcc ttctcaggtc gtatctggca
     3841 aaaaacagat tcacaaactt ctaagtataa aaacagccga tcgactgcaa tttttaacaa
     3901 aagttacgaa ttatatcata tggatagggc aaaaaaatct tctggaaaaa ctagtgagat
     3961 ttacctgatg gaaggattca tggatgttat tgcagcctat cgggctggaa tcgaaaatgc
     4021 tgtggcgtcg atgggaacgg ccttgagtcg agagcatgtt gagcatctga aaaggttaac
     4081 caagaaattg gttcttgttt acgatggaga taaggctggg caagccgcga tattgaaagc
     4141 attggatgaa attggtgata tgcctgtgca aatcgttagc atgcctgcta acttggatcc
     4201 tgatgagtat ctacaaaaaa atggtccaga agacttggcc tatctattaa cgaaaactcg
     4261 tattagtccg attgagttct acattcatca gtacaaacct gaaaacagtg aaaatctgca
     4321 ggctcagatt gagtttattg aaaaaatagc tcccttgatt gttcaagaaa agtccatcgc
     4381 tgctcaaaac agctatattc atattttagc tgacagtctg gcgtcctttg attatgccca
     4441 gattgagcag attgttaatg agagtcgtca ggtgcaaagg cagaatcgca tggaaggaat
     4501 ttccagaccg acgccaatca ccatgccaat caccaagcag ttatcggcta ttatgagggc
     4561 agaagcccat ctactctatc ggatgatgga atcccctctt gttttgaacg attaccgttt
     4621 gcgagaagac tttgcatttg ctacacctga atttcaggtc ttatatgact tgcttggcca
     4681 gtatggaaat cttcctccag aagttttagc agagcagaca gaggaagttg aaagagcttg
     4741 gtaccaagtt ttagctcagg atttgcctgc tgagatatcg ccgcaggaac ttagtgaagt
     4801 agagatgact cgaaacaagg ctctcttgaa tcaggacaat atgagaatca aaaagaaggt
     4861 gcaggaagct agccatgtag gagatacaga tacagcccta gaagaattgg aacgtttaat
     4921 ttcccaaaag agaagaatgg agtaataatg gcaacaaaac aaaaagaagt aacaacattt
     4981 gacgtacagg tagcagaatt tatccgtaat cataagcaaa aagggacagc gacagatgat
     5041 gaaatcaatg caagtctggt tattcctttt accttggacg ctgatgggat tgaagatctc
     5101 ttgcaacgga ttcaggatgc aggcatttct atcacagata acgaaggaaa tccaagtgcg
     5161 cgtgttctta gcaatgaaga agaaccagaa ctcagcgatg aggacttgat tgggtcaact
     5221 tctgctaagg tcaatgaccc tgtccgtatg tacttgaaag aaataggggt cgttcctctc
     5281 ttgaccaatg aagaggagaa agagttggca ctggctgttg aagctggcga tatcgaagcc
     5341 aaacaacgtc ttgcggaagc caatcttcgt ttggttgttt ccattgccaa acgctatgtc
     5401 ggtcgtggca tgcagttcct tgacttgatt caagaaggaa atatgggctt gatgaaggcg
     5461 gttgacaagt ttgactattc taaagggttc aagttttcaa cttatgcaac ttggtggatt
     5521 cgtcaggcta tcactcgtgc tattgcggat caagctcgta ccatccgtat cccagttcac
     5581 atggttgaaa ctatcaataa attggttcgt gaacagcgga atctccttca agaattgggg
     5641 caagatccga caccagaaca gattgctgaa cgaatggata tgacacctga taaggttcgt
     5701 gaaatcttga agattgccca agaaccagta tctcttgaaa ctcctatcgg tgaagaggac
     5761 gatagcaacc ttggagactt tatcgaagat gaagtgattg aaaatccagt ggattatacg
     5821 actcgtatcg tcttgcgtga gcaattggat gaaatcttag atactcttac agaccgtgaa
     5881 gaaaatgttc tgcgtctacg ttttggacta gatgatggaa aaatgcgcac acttgaagat
     5941 gtggggaaag tctttaacgt aactcgtgag cgtatccgtc agattgaagc aaaggctttg
     6001 agaaaactac gccaaccaag ccgcagcaaa ccgcttcgtg attttattga agactaagag
     6061 tgaggaaaat atggcttata cagaagagca aattgaaaac atcaaaacac ggattttaac
     6121 agccttggaa gaagtcatcg accctgagtt gggaatcgat attgtcaatc ttggtttgat
     6181 ctatgagatt cgttttgacg gtgacacagg gcaaacagag attgacatga ctttgacaac
     6241 tatgggttgt cccttagcag accttttgac agaccagatt tatgatgcta tgatagaggt
     6301 tccagaagta acggatactg aggttaaatt agtctggtat ccagcctgga cagttgaaaa
     6361 aatgagtcgc tatgcccgca ttgccctagg cattaagtaa acatataaaa gcatgtgaga
     6421 gactagagga aaagcgagga aatttcccct cttttcctct agtctctcct ttcttttgct
     6481 gattttattc aaagaaaatg atataatagt agttatggag aaaaagaaat tacgcatcaa
     6541 tatgttgagt tcaagtgaga aagtagcagg acagggagtt tcaggtgctt accgtgaatt
     6601 agttcgtctt cttcaccgtg ctgccaagga ccaattgatt gttacagaaa atcttccaat
     6661 cgaggcagat gtgactcact ttcatacgat tgattttccc tattatttat caaccttcca
     6721 aaagaaacgc tcagggagaa agattggcta tgtgcatttc ttgccagcta cacttgaggg
     6781 aagtttgaaa attccatttt tcttaaaggg aattgtgaaa cgctatgtat tttcttttta
     6841 caaccggatg gagcacttgg ttgtggtcaa tcctatgttt attgaggatt tggtagcagc
     6901 tggtattcca cgtgaaaaag tgacctatat tcctaacttt gtcaacaagg aaaaatggca
     6961 tcctctacca caagaagagg tagttagact gcgcacagat cttggtctta gtgacaatca
     7021 gtttatcgta gtaggtgctg ggcaagttca gaaacgtaaa gggattgatg actttatccg
     7081 tctggctgag gaattgcctc agattacctt tatctgggct ggtggcttct cttttggtgg
     7141 tatgacagat ggttatgaac actataagaa aattatggaa aatcccccta aaaatttgat
     7201 ttttccaggc attgtatcgc cagagcggat gcgcgaattg tatgctctag cggatctttt
     7261 cttgttgcct agttacaatg agctctttcc tatgactatt ttagaagctg cgagttgtga
     7321 ggctcctatt atgttgcgtg atttagatct ctataaggtg attttggagg gaaattatcg
     7381 ggcgacagcg ggtagagaag agatgaaaga ggctattttg gaatatcaag caaatcctgc
     7441 tgccttaaaa gatctcaaag aaaaggctaa gaatatttcc agagagtatt ctgaagagca
     7501 tctgttacaa atctggttgg acttttatga gaaacaagcc gctttaggga gaaagtaaaa
     7561 agtgaggtaa tctatgcgaa ttggtttatt tacagatacc tattttcctc aggtttctgg
     7621 tgttgcgacc agtattcgaa ccttgaaaac agaacttgaa aagcagggac atgctgtttt
     7681 tatctttacg acgacagata aggatgtcaa tcgctacgaa gattggcaaa ttatccgcat
     7741 tccaagtgtt cctttctttg cttttaagga tcgtcgcttt gcctaccgag gttttagcaa
     7801 ggcacttgaa attgctaaac agtatcagct agatattatc catactcaga cagaattttc
     7861 tcttggcctg ttggggattt ggattgcgcg tgaattgaaa attccagtca tccataccta
     7921 tcacacccag tatgaagact atgtccatta tattgctaag gggatgttga tccggccgag
     7981 tatggtcaag tatctggtta gaggtttcct gcatgatgtg gatggggtta tttgccctag
     8041 tgagattgtc cgtgacttgc tatctgatta taaggtcaag gttgaaaaac gggtcattcc
     8101 tactgggatt gaattagcca agtttgagcg tccggaaatc aagcaggaaa atttgaaaga
     8161 actgcgtagt aaactaggga ttcaagatgg tgaaaagacg ttgcttagtc tttcgagaat
     8221 ctcctatgaa aaaaatattc aagcagtttt agtagccttt gctgatgttc tgaaagagga
     8281 agataaggtt aaactggtag tagctgggga tggcccttat ctgaatgacc tcaaagagca
     8341 agcccagaac ctagagattc aagactcagt catctttaca gggatgattg ctcctagtga
     8401 gacggctctt tactataaag cggcggattt cttcatttcg gcatcgacaa gcgaaacgca
     8461 aggtttgacc tacttggaaa gcttagccag tggaacacct gtcattgctc acggaaatcc
     8521 ttatttgaac aacctcatca gtgataaaat gtttggaacc ttgtactatg gagaacatga
     8581 tttggctggt gctattttgg aagccctgat tgcaacacca gacatgaacg agcatacctt
     8641 atcagagaaa ttgtatgaga tttcagctga gaactttggg aaacgagtgc atgagtttta
     8701 tctggatgcc attatttcaa ataacttcca gaaagatttg gctaaagatg atacggtcag
     8761 tcagcgtatc tttaagacag ttttgtatct tccgcaacag gtggttgctg tacctgtaaa
     8821 aggatctaga cgcatgttga aggcttcaaa aacacagttg atcagtatga gagactattg
     8881 gaaagaccat gaagaataga aagaggaaca gctatgaaaa aaacaattaa tgagaagcgg
     8941 tcgtgataaa aagattgcgg gtgtttgtgc tggggtggcc cattatctgg atatggatcc
     9001 gactatcgtt caagtcattt ggggtgttct tacttgctgt tacggagctg gaattgtagc
     9061 ttacattatt ttatggatta tcgcaccagt agcaactgac tattgaaaac tagctcaatt
     9121 catgctaaga taatagaaaa tagaatgtaa cgaaggagag aaaaatggca tttggagata
     9181 atggaaatcg taaaaaaact atgtttgaga aaataacctt gtttatcgtg attatcatgc
     9241 tagtagcaag tttattggga atttttgcaa ctgcaattgg tgccctcagt aatctataaa
     9301 atagattcaa gaaaatttag tgactgggat ttcccagccc ttttttaaag tgagaagaaa
     9361 taatgagtat gtttttagat acagctaaga ttaaggtcaa ggctggtaat ggtggcgatg
     9421 gtatggttgc ctttcgtcgt gaaaaatatg tccctaatgg aggcccttgg ggtggtgatg
     9481 gtggtcgtgg aggcaatgtg gtcttcgttg tagacgaagg actacgtacc ttgatggatt
     9541 tccgctacaa tcgtcatttc aaggctgatt ctggtgaaaa agggatgacc aaagggatgc
     9601 atggtcgtgg tgctgaggac cttagagttc gagtaccaca aggtacgact gttcgtgatg
     9661 cggagactgg caaggtttta acagatttga ttgaacatgg gcaagaattt atcgttgccc
     9721 acggtggtcg tggtggacgt ggaaatattc gtttcgcgac accaaaaaat cctgcaccgg
     9781 aaatctctga aaatggagaa ccaggtcagg aacgtgagtt acaattggaa ctaaaaatct
     9841 tggcagatgt cggtttagta ggattcccat ctgtagggaa gtcaacactt ttaagtgtta
     9901 ttacctcagc taagcctaaa attggtgcct accactttac cactattgta ccaaatttag
     9961 gtatggttcg cacccaatca ggtgaatcct ttgcagtagc cgacttgcca ggtttgattg
    10021 aaggggctag tcaaggtgtt ggtttgggaa ctcagtttct ccgtcacatc gagcgtacac
    10081 gtgttatcct tcacatcatt gatatgtcag ctagcgaagg ccgtgatcca tatgaggatt
    10141 acctagctat caataaagag ctggagtctt acaatcttcg cctcatggag cgtccacaga
    10201 ttattgtagc taataagatg gatatgcctg agagtcagga aaatcttgaa gaatttaaga
    10261 aaaaattgtc tgaaaattat gatgaatttg aagagttacc agctatcttc ccaatttctg
    10321 gattgaccaa gcaaggtctg gcaacacttt tagatgctac agctgaattg ttagacaaga
    10381 caccagaatt tttgctctac gacgagtccg atatggaaga agaagcttac tatggatttg
    10441 acgaagaaga aaaagccttt gaaattagtc gtgatgacga tgcgacatgg gtactttctg
    10501 gtgaaaaact catgaaactc tttaatatga ccaactttga tcgtgatgaa tctgtcatga
    10561 aatttgcccg tcagcttcgt ggtatggggg ttgatgaagc ccttcgtgcg cgtggagcta
    10621 aagatgggga tttggtccgc attggtaaat ttgagtttga atttgtagac taggagactg
    10681 gtatgggaga taaaccgata tctttccgag atgcggatgg taattttgtt tccgccgcag
    10741 acgtttggaa tgaaaagaaa ttggaagaac tatttaatcg tctcaatcca aatcgtgcct
    10801 tgagattggc acgaactaaa aaggaaaatc catctcagta aagaagc
//
LOCUS       DAVDWM010000058        10843 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVDWM010000058 DAVDWM010000000
VERSION     DAVDWM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.058"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(7..1221)
                     /locus_tag="VUW49_001879"
     CDS             complement(7..1221)
                     /locus_tag="VUW49_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7981752.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1377..1670)
                     /locus_tag="VUW49_001880"
     CDS             complement(1377..1670)
                     /locus_tag="VUW49_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981753.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1832..2284
                     /locus_tag="VUW49_001881"
     CDS             1832..2284
                     /locus_tag="VUW49_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7981754.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2319..3707)
                     /locus_tag="VUW49_001882"
     CDS             complement(2319..3707)
                     /locus_tag="VUW49_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7981755.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3773..4735
                     /locus_tag="VUW49_001883"
     CDS             3773..4735
                     /locus_tag="VUW49_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET7981756.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQTLKDYIEG"
     gene            complement(4764..5057)
                     /locus_tag="VUW49_001884"
     CDS             complement(4764..5057)
                     /locus_tag="VUW49_001884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981757.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVGSKHVFEVVDETNEVS
                     SKHCFEVADETDEVSSKHCFEVADETDEVSSKHVFEVVDETDEVSNHTYGKAT"
     gene            complement(5121..6131)
                     /gene="ccpA"
                     /locus_tag="VUW49_001885"
     CDS             complement(5121..6131)
                     /gene="ccpA"
                     /locus_tag="VUW49_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET7981758.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6556..7155)
                     /locus_tag="VUW49_001886"
     CDS             complement(6556..7155)
                     /locus_tag="VUW49_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981759.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQTKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7157..8254)
                     /locus_tag="VUW49_001887"
     CDS             complement(7157..8254)
                     /locus_tag="VUW49_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7981760.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLSSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHIFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKAFKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(8254..8991)
                     /locus_tag="VUW49_001888"
     CDS             complement(8254..8991)
                     /locus_tag="VUW49_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981761.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(8993..9877)
                     /locus_tag="VUW49_001889"
     CDS             complement(8993..9877)
                     /locus_tag="VUW49_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981762.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9864..10058)
                     /locus_tag="VUW49_001890"
     CDS             complement(9864..10058)
                     /locus_tag="VUW49_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981763.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIHGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(10055..10261)
                     /locus_tag="VUW49_001891"
     CDS             complement(10055..10261)
                     /locus_tag="VUW49_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981764.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         3315 a         2459 c         1939 g         3130 t
ORIGIN      
        1 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
       61 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
      121 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
      181 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
      241 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
      301 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgccccc ccaaggaagt
      361 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
      421 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
      481 aaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
      541 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
      601 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
      661 cttaggataa agggctccag ttgggttatt tggattgata aggacgattg ccttggtatt
      721 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattcaacag cttcatcaca
      781 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
      841 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
      901 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
      961 cagctggcaa tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata
     1021 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
     1081 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
     1141 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
     1201 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
     1261 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
     1321 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggt attttgcaaa gacacatcac
     1381 ataccaaaag ataagcctag aaaaagcaag cctagaaagg agtcataaag aagattaaac
     1441 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
     1501 aataagatta gatggtagag tacatgaaag actaaaaatc caataaaact tggataaaga
     1561 agcggtacca gactctctaa ttgacagatc atgatcactt ttaccaagac tgaaactagg
     1621 ataattccat aatacaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
     1681 ttcacttctt tccttctatc ctttctttta ttttatcata tatttatata gattttaagc
     1741 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
     1801 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
     1861 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
     1921 acgaaacgac gctaaactca ccatcgcaca tgtcattgac acacgcgctc tccaaagcgt
     1981 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
     2041 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
     2101 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
     2161 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttggtcggct cttcatctga
     2221 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
     2281 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
     2341 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
     2401 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
     2461 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
     2521 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
     2581 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
     2641 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
     2701 gctgctcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
     2761 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
     2821 ttgaccttat tgaaatgata gtcacggctg acaaagactt tttctgaaga caaaacacca
     2881 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
     2941 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
     3001 atttcaaggt cattatctga gtcaccaaaa gccatgactt gattgaggtc aaagccatat
     3061 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
     3121 gcaaaaggat tgctacgtgt caatttcaag tcttcaaaat cagctgccgc cttctcagat
     3181 tcttctggtg tcatcagcat caaaacttgg tagataggct gattcatcag gtgaagcagg
     3241 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
     3301 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
     3361 gacatgattt tagaacccaa catggcatcc ttggtcccta gagcaatctc cgtgccctct
     3421 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
     3481 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
     3541 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
     3601 ccttgttctt tgacaatctt aatcgcatcc ttagtggatt tcaaaacact cttgcgattg
     3661 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct atcccaatct
     3721 ccccttttgt gatacaatga ttataccaca tttcagaaag agtgagtaaa tcatgcctaa
     3781 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
     3841 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
     3901 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
     3961 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg tgatcacaca
     4021 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
     4081 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgag ctcggtagtg atggtgttta
     4141 taattatcta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
     4201 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
     4261 tgtcagcacc ttccagactc caacacatgg ccccctcggt ctcatcatga aacaggaaat
     4321 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
     4381 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
     4441 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
     4501 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaatccca gtcgctctgg tatcacgatg
     4561 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
     4621 agcaggcgtt ttctttgtta aagaactcaa tgcccaaaaa gctcgcttga aactcctcat
     4681 cgccctcaat gccggactaa caggacagac tttgaaagac tatatagaag gctaatactc
     4741 ttcgaaaatc tctgcaaacc acgtcacgtc gccttaccgt atgtatggtt actgacttcg
     4801 tcagtttcat ccacaacctc aaaaacatgt tttgagctga cttcgtcagt ttcatctgca
     4861 acctcaaagc agtgctttga gctgacttcg tcagtttcat ctgcaacctc aaagcagtgc
     4921 tttgagctga cttcgttagt ttcatctaca acctcaaaaa catgttttga gccgacttcg
     4981 tcagttctat ctacaacctc aaaacagtgt gttgcgcagc ctttaaccag ccgcctagtc
     5041 cgctctatgg tattcattaa gtcaacatct cttgtttaag agcacaaaat caggaaatct
     5101 tctcgattcc ctgatttttt ctatttacgt tttcgtgttg agctacgttc tgtcaaacca
     5161 tgaggtaaga gaacttcacg ttcttccaac tcttccttat gcataatctt ggtcaacata
     5221 cgcatactaa tggcaccaag gtcataaaga ggttgggcaa tcgttgtcaa gtttggacgg
     5281 gtaaagcgtg agatttgtga atcatcacta gtaataattt caaaatcttc tggcacagaa
     5341 acacctttat cagccaaacc gttcaagact cctgctgcca actcatcacc tgtcacaact
     5401 gctgcagttg catttgatga aatcaaacgc tctgctaagg cgtaaccatc atcatagcta
     5461 tatttagatt caaataccaa accctcacta taagtgattc ctgctttttt caaggtttcc
     5521 ttgtagccaa ctaaacgaac cttaccattg atgtcatcca ctagcggacc gctaacgaaa
     5581 gcaatacgct cattttcttt agcaaggtaa ctcactgcat caattgttgc ttgcttatag
     5641 tcaatattga cacttggcaa ctggtgctca acatcgacag ttcctgcgag aacaatcgga
     5701 gtacgtgaac gcgaaaattc tgagcgaatt ttatctgtca agtgataacc catatagata
     5761 atgccatcta cctgctttga aaagagggta ttgacaacag aaacttcttt ctcgttatct
     5821 tcatcgctat tagctaggac aatattgtac ttgtacattt ctgcaatatc atcaatcccc
     5881 ttagccaaac tcgaaaaata accattggta atatttggaa tcacgacacc gacagtggtt
     5941 gtctttttac ttgcaagacc acgcgcaact gcatttggac gataatccaa acgatcaatt
     6001 acctctagca cttttttacg ggtattctct tttacatttt tattgccatt gaccacacgg
     6061 ctgaccgtcg ccatggaaac acctgcttca cgagcgacat cataaatggt tactgtatca
     6121 tctgcattca ttccttttcc tgtcctttct atctcacaca ttcttttaca agtagaggtg
     6181 ctgattgaag ctctatatct acttacaaaa gtgaagatgt gaaaatttcg ttttcatatt
     6241 tctacttatt ccattctatc actaattgta aacactttca agtgtttttt gaagattgat
     6301 tgaaaaaatt tcatagaaaa cctaggttta gctccttgct accaccttag actaaacaaa
     6361 aaggaggaaa ctaagccctc ctaaagttat agtaaaatga aataagaaca ggataaatcg
     6421 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
     6481 ttcaatctac tataggtatt gttccattca ctaccgtcaa ttttagcaca tagtcttcat
     6541 aaaaatatta tatcatcata accaaccaga ttcttttgcg atattagctg cctctgttcg
     6601 attaccagca tccagttttg aaagaatatt ggtgacatag tttcgaatag ttccgtttga
     6661 gaggtaaagc tgatctgcga tttcttggtt ggataagccc cgggcgattc cctttaagac
     6721 agcaatctct tgttctgtca acggattggg acgggtcatc accacttcca tcaattcagg
     6781 cgaatactcc ttgcgtcctt cgaggacggt gtgcaaggtt tgcatgaggt ctgcaatgct
     6841 tcgttccttt aatacataag catccactcc agccttgacc gcacgttcaa aatacccagc
     6901 acgcttgaag gtcgtcacca caaccacctt tgtttcaagc ttttctgatc gtatccactc
     6961 caagacttca agacctgtct taacaggcat ttctacgtca aggatggcga tatctacaga
     7021 ctccttttct aatagttgga ttgcttcttg cccattcttg gtttgaagga cagactccac
     7081 atctggttga agcgtgagca attgacacat ggcatctcgc aacatacttt gatcttctgc
     7141 gactaatact ttcatcttag tttctctcct tataaggtag tcgaacttgc acttccgttg
     7201 gatgtttctg actgattaca cttacttctc ctgaaaatgg aaaaacacga tctcggactg
     7261 tatggagctc atctcctttt agaaaagcaa agccgcagcc atcatcactt actgttaaaa
     7321 tgagttcctt ctctgtccgt tctaatttta agtaagcttt aaacgctttg gcatgtttga
     7381 tgatattggt caccaactca agcaaaatca tggaagccat tgactccaat tcttgagtaa
     7441 ggctagcagt atctagttgg ttatccgttt ccacctcaat tccagcaatt tctaacatct
     7501 ttttcacagt ctctagttcg gatgtcaaag ttctagactt aagattttcc acaatggttc
     7561 gaacttcacg catcgattct ttgctaattt gctgtatttc tcttaattcc ttttccactt
     7621 gtggataagc ctgcatttga aatagctgca aggctagatc tgtcttgaca ctaagcatag
     7681 caaagatatg tcccagacta tcatgcaaat cctgaccgat acgattgcgc tcattttctg
     7741 ctagcaatag atttatctgg gtattttgct tgacctgagc ttctttcaaa tcctcgacaa
     7801 ggcgaattcg aaccgagctc aaagtcatca aatcgacaaa agtaagaatt acaagcatat
     7861 agactagaaa ctcaacttcg attctctgaa aaatcaaaag tcgccccaca acaaggactt
     7921 gagcaaggag aaatgtccat acatgtaaag actttaaact acgtacgcca aaatgataaa
     7981 ttaagagatt ggaaaggaaa aagaaaaacc agatataatt cccagcaaca aaggcggtat
     8041 tcccagctac atagaccagc atgatccccc agaaaagcca agataggcgc tggttcttag
     8101 ttattaaaac acccaaatac gtcactacaa atagaatatc aaccaataaa tgccaagcag
     8161 gaagctcccc aatcactaca ggtaggatgg gaaaaagcat aaaaatcaaa ctggcccaaa
     8221 acatatagtg tatgcttttc agtctttcaa gcattaagca ttctccttat gaccttgaag
     8281 gtaaatagtc aaaccaaata aaactgctga aaaaacaagt aaataaactg tggctgatag
     8341 attgatgcca ccctcgttta agaaggtctt gagcaactcc atcaattgat aggtcgggag
     8401 acacttacca actgcttgca tccagtctgg aaataaagaa atgggcatcc agagtccgcc
     8461 taaaacagcc aatcctagat aaagaagatt gcccacaaca gacatcagct gactagttgg
     8521 taagagagtc aaggtcaagc caagtgctac aaaagcaata ctccccacaa tcagcaaaat
     8581 cgcagctcca acccatttta ctagaggcat gtctacacct cttacaaaat gtccaactgt
     8641 aaaaacaacc agaattgaga ccaaataatc aaccagcata cttgttatct ttgatagata
     8701 atattctacc atatttacag ggctatgacg caatgttttc tgccagttgt tcatcttatc
     8761 ggtatgtaaa acagctggaa atgagaggat agttgtcgac atcatggaaa aggcagtcat
     8821 ggagatgagg taatcacgca taaaattcgc gggttcacct ggtgtgtcct gatagatacc
     8881 agaaaaaaat aaatagaaag ccgtcggcat ccctaccgac aatagataat agattaattg
     8941 tcgtttggtc aataaaaatt ctatcttact aagtgctatc catcgtttca tcttagttat
     9001 ctcccttctg cgtctcttca aagattgtat tcaacaaact acgattatta acttcgattt
     9061 cttgcatcct acatcctgct tgggctaata gttcccagaa agcatctgct tctcgagtga
     9121 ctacttgtaa agaatcttgc tttagggtcc agttttcaac caaatttgac tgctctacga
     9181 cttccttgta agctatagga aggataaagt gcttttctat ttcctcgcta cgcatagcta
     9241 gaggcgtcgt atctcgaatc aactctccct tatttaagag caaaatgcgg tctgcagtgt
     9301 gttccacttc ctcaatataa tgggacgaat agagaatggt gactccttgc gcttttaact
     9361 cctggacaat ttcccaaaaa cgttgacgag ttgaggtatc catggacgca gtaggctcat
     9421 ctaaaaaaac aatctttggt cgcccaatta aggtcaagac gaaagaaaag agacgctttt
     9481 gaccgcctga taatttttct gccaactgtt ctttttgttg cttgacaaac tgcaatagtt
     9541 gatcaatttc ctgattgctc aaaggatttg gatagatacg ttgaaagaaa gcaatcaact
     9601 ctttaacttt taatttctga acgattacat tttcttgagg gaggtaagcc ctagtatagt
     9661 ctaacttaga actcgtcact ggtaaacctt gaatagatac ttgaccgctt gtgaccagtt
     9721 tatctccaag caaacaatct aagagtgttg tctttccggc accatttgga ccaattaagg
     9781 caacacattc accatcgttg atttcaaagg agatgttccg caaaatttcc ttgtctttta
     9841 ttttcttact caatttttca actttaacca cagtcatgag actctccttt caaccactcc
     9901 attcccatag ggaaaacgac gaaaatcata aatccaaacc ccaaagcacc atgaatgaat
     9961 tggcgaagca aggcttgatc aaaccaatca gtaaacattt ccactaacca taccaagagt
    10021 gacaggccga taaagaaata gatgatccct ctcttcattc ttcaaactcc tttttcacat
    10081 ctctgactaa tttcaaacct tctctaacaa gccaagacat gattccaaag ccagcaaaga
    10141 ccttccaagg aaaatggtag aaatcctcat ccaatcccga aaacatgaga taggtcataa
    10201 ctcctgctac tactaaactc attgcaataa tcattttatt tctcatctct tcttccccca
    10261 tttcatacta caattatagt cttttaaaat cagaggaggt agaagcttct gtcactagaa
    10321 aatatgacaa atgtcataaa aaattctgtt caaaacaagc aagatacact atacaataaa
    10381 acacaatcag aaaaatctaa ggcaacttcc tcaaaagaga tatcaaactc aattcacacc
    10441 ataatgtaaa ctaatactta tttaaaatca aaaagagtag aaatttttat cagacaaaca
    10501 catatatagt gtattgaatc tataacagta ggccttaaat actaaaatat ttctataaat
    10561 taatttaact ttcctgatag agctgttcat atcttatttc aattctctat attatacgtt
    10621 gaacaaaacc cttctattgc tttcttaaaa atttataaga gttataaaat ctgttaaatt
    10681 tcaatgtgta tacctaaact acggtattta ttgaaaagac tggagacaaa aagtatacgc
    10741 tgccaaaatg aattactgaa aatcaaaaaa gagagaacca aactgattcc ctcttaatgt
    10801 atataatatc tagtttttaa aaaacacact cacatatctc tgt
//
LOCUS       DAVDWM010000059        10222 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVDWM010000059 DAVDWM010000000
VERSION     DAVDWM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.059"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            96..329
                     /locus_tag="VUW49_001892"
     CDS             96..329
                     /locus_tag="VUW49_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET7981765.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            329..1663
                     /locus_tag="VUW49_001893"
     CDS             329..1663
                     /locus_tag="VUW49_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET7981766.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEVFEAFAIEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1673..1927
                     /locus_tag="VUW49_001894"
     CDS             1673..1927
                     /locus_tag="VUW49_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET7981767.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            2023..2418
                     /locus_tag="VUW49_001895"
     CDS             2023..2418
                     /locus_tag="VUW49_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET7981768.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            2831..3280
                     /locus_tag="VUW49_001896"
     CDS             2831..3280
                     /locus_tag="VUW49_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HET7981769.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3267..3827
                     /locus_tag="VUW49_001897"
     CDS             3267..3827
                     /locus_tag="VUW49_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981770.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            3827..4405
                     /locus_tag="VUW49_001898"
     CDS             3827..4405
                     /locus_tag="VUW49_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7981771.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            4398..7049
                     /locus_tag="VUW49_001899"
     CDS             4398..7049
                     /locus_tag="VUW49_001899"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7981772.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPVFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            7130..7294
                     /locus_tag="VUW49_001900"
     CDS             7130..7294
                     /locus_tag="VUW49_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HET7981773.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            7345..8949
                     /locus_tag="VUW49_001901"
     CDS             7345..8949
                     /locus_tag="VUW49_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7981774.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWELYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQE
                     QRRAVSKIQPESSMFQCIKEKLEALF"
     gene            9329..10012
                     /locus_tag="VUW49_001902"
                     /pseudo
     CDS             9329..10012
                     /locus_tag="VUW49_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         3182 a         1864 c         2232 g         2944 t
ORIGIN      
        1 acaacctcaa aacactgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt
       61 ttcattgagt attagaacat ataatggagg tcgtcatgga caatatcatc gatgtgtcaa
      121 ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta gtggagctgg
      181 gttttaaacc ccttgccaat cccttgatgc gcaacacagt cggtcgcaag gtatcgctca
      241 aacaaggttc taagctagca ggaactccta tggacaagat tgtacgcaca ctggaagcga
      301 atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg tcctacggga
      361 tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc gttttgatgc
      421 gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc tgatgaactc
      481 agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg ccaatctttt
      541 taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc acccagttcg
      601 tgtctttaag gaagaaaatt tggccctccg tgcagctttg attcgcattc gtagattgtt
      661 agatacctat gagttgatgg aagacgagga aatgcttgcg gagatgcgca agggcttggt
      721 gcgtcagatg ggacttgtgg ggcaatttga catccattac caacgtaaag aagaactctt
      781 ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt ggggagtgga
      841 tgatcagatt agggaactct ttcaaacagc tctaacgaca gccaagtcac taccagaagt
      901 gtcaattagc agtgtaaagg aagtttttga agcttttgcg atagagtttg aaagtatgat
      961 tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc aggatgactg
     1021 gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc cgtcagagaa
     1081 atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc ctgtacagct
     1141 agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc attttaccat
     1201 tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac aggcttttgg
     1261 taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcatctcc ctatggagat
     1321 tacctttgtc aataaagaag atattttcca gtattacaat gacaatacgc cagctgatga
     1381 gatgattttc aaacggacgc cgtctcaagt cgggcgcaat gtcgaactct gccatccgcc
     1441 taagtacttg gacaaggtca aaactatcat gaagaggctt cgtgagggaa gcaaagacaa
     1501 gtatgaaatg tggttcaagt ctgagtcgcg aggtaagttt gtccacatca cctatgctgc
     1561 agtacacgat gaagacggag aattccaagg agtgttggag tatgttcagg atatccagcc
     1621 ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa aaatgagtta
     1681 cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca tgattaacga
     1741 catggcgcac aaggaaagcc aaaaagttta cgaagaagac caggacgagc gtgccaaaga
     1801 tgccatgatt cgctatgaga gtcgcttgga tgcttatcag ttcttgcttg gtaagtttga
     1861 aaacttcaaa gtaggcaagg gattccatga tttgccagaa ggcttgtttg gtgagcgaaa
     1921 ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg tttatgttaa
     1981 atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa gaaactcaat
     2041 cgtaaaaaac aattacgaaa tggcctccgt cgcgcaggtg ccttttcaag tactgtgact
     2101 aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc aagcgcagct
     2161 ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga gcaagctcaa
     2221 aaagtagcta attctgtaga agattttgca gcaaatttgg gtggacttcc acttgatcgt
     2281 gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa ctggactgaa
     2341 aaagaactcc ttgccttgaa aggaatcggc ccagctacca tcaagaaatt gaaagaaaat
     2401 ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgaaaaaatc ttgctacaat
     2461 agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg ctgagaagtc
     2521 cacaaatgtg tcaaaactgg ttgctaatgg atgaaaaatt gaaataaaag tgtctttttg
     2581 ctttaaagac gagagttgcg ggcaactgcc cgaaattggg tggtaccgcg gataaacaca
     2641 ttcgtccctg tcatgtagat ggcagggctt ttcttttacg tcttagtcaa agggggttgt
     2701 tatgaagcaa tcttttaaca caagtaaact atattatggt tttcctgttt ttattttagg
     2761 atatcaagat tagaattttg gctataacat cacgacctgt agttcctctt atagtctagg
     2821 agattgggtt gtgattggag tcgttgcgag agagaatgcc gcagagcaga tcaaacagta
     2881 tcaaaaattt actgtgaata tttctgatga aacttctatg cttgcgatgg agcaggctgg
     2941 ttttatcagt catcaggaga aattggaacg tttgggagtg cattatgaaa tttctgaacg
     3001 aactcagact cctattttag acgcctgtcc acttgtttta gattgtcggg tagataggat
     3061 tgttgaggaa gacggtattt gccacatctt tgccaagatt cttgagcgac ttgttgttcc
     3121 agaactcctg gatgaaaagg gacattttaa aaatcaactg tttgccccaa cctatttcat
     3181 gggagatgga tatcagcgcg tttatcgcta tctggacaag cgtgtagata tgaagggcag
     3241 tttcatcaaa aaagcgagga agaaggatgg caagaactga gctgccagat aaaatcgaaa
     3301 cagaacgtct cgttttacga gtccgtactg tggcggatgc tgaggatatc tttgactatg
     3361 ctagtttgcc agaggtcgcc tatccagcag gttttcctcc agtcaagacc ttggaagatg
     3421 agatttatta tctggagcac attcttccgg agcgtaatca aaaggaaaat ctcccagcag
     3481 gctacgggat tgtcgtcaaa ggaacggata agatcgttgg ctctgtcgat ttcaaccatc
     3541 gccatgaaga tgatgtgctg gaaatcggct ataccttaca cccagactat tgggggcgag
     3601 gttatgtgcc agaagctgcg cgtgccttga ttgacttagc ctttaaagat ttgggtcttc
     3661 acaagattga actaacttgc tttggatata accttcaaag taaacgagtc gcggaaaagc
     3721 ttggctttac cctcgaagct cgcataagag accgcaaaga tgctcaaggc aactgctgtg
     3781 atgatttaag atatgccttg ctgaagagtg aatgggaggt gatttgatgc atcttttcat
     3841 cattggtgct ccagcatcag gaaaaatgac cattggtcaa gaattatctc gactgacgga
     3901 tgctacccta ttttataacc atcaagccat cgattttgca ctagaaatct atcaggacta
     3961 tacagaggaa atgtgggaat ttgttcgtgg aatcactttt tcattccttg gagcgagtgc
     4021 aagaaatcag cgatcagtaa ttttaacaga tgtaattgat ttttcaaatc agtatcatct
     4081 gatatattta aagcaaattc agagtttgtt ggatgattat catcaagaga ttctttttgt
     4141 tgagttggaa acaagtcttg aagagtgcat acgtcgaaat cgaacggaga atcgattaaa
     4201 gtacaaaccc ttaaaacgac atattgaggt atctgaaaga gaaattatag agaccgctga
     4261 aacacttcaa ttaaattccc aatatcaacc gaatgagttg caccactact ttaaaataaa
     4321 taatacgaat ttgtctgcag aagaagctgc taagcaaatt caaaataaaa taaacaaaat
     4381 agagaaagga cacacacatg tctaaagaac tttcatctaa atacaatcca gccgaggttg
     4441 aggctggtcg ttaccaaaaa tggcttgatg ctgatgtttt caagccttca ggcgatcaaa
     4501 aggctaagcc ttattcaatc gttattccac caccaaacgt tacaggtaaa cttcaccttg
     4561 gtcacgcttg ggatacaact ttgcaagata ttatcatccg tcaaaaacgc atgcaaggtt
     4621 ttgataccct ttggcttcct gggatggacc acgcagggat tgccactcag gctaaggtag
     4681 aggagcgctt gcgtggtgag ggcatttccc gctatgacct tggtcgtgag tctttcttga
     4741 cgaaagtctg ggaatggaaa gacgaatatg ccactactat caaggaacaa tggggcaaga
     4801 tggggctctc tgtagactac tctcgtgagc gtttcactct tgacgaaggt ttgtcaaaag
     4861 ctgttcgtaa ggtctttgtg gacctttaca agaaaggctg gatctaccgt ggtgagttta
     4921 tcatcaactg ggacccagca gctcgcacag ccctttctga tattgaggtg attcacaagg
     4981 atgtagaagg tgccttctac cacatgaatt acatgctgga agatggttca cgcgcccttg
     5041 aagttgctac aactcgtcct gagaccatgt ttggggacgt tgcggttgcg gttaatccag
     5101 aagacccgcg ctacaaggac ttgattggta aaaacgtcat ccttccaatc gctaataaac
     5161 tcatcccaat cgttggagat gagcacgcag atcctgagtt tggtactggt gtcgtgaaaa
     5221 tcacacctgc ccacgatcca aatgacttct tggttggcca acgtcataac ttgccacaag
     5281 tcaacgtcat gaacgacgac ggaactatga atgagcttgc ctttgaattt tcaggcatgg
     5341 atcgttttga agctcgtaag gcagtcgttg ctaagttgga agaaatcggt gcccttgtca
     5401 aaatcgaaaa acgtgtccac agtgttggtc actcagaacg tacaggtgtc gtagttgagc
     5461 cacgcttgtc tactcaatgg ttcgtcaaga tggaccaatt ggctaagaac gccattgcca
     5521 accaagacac agaggacaag gtcgaattct acccacctcg tttcaacgat accttccttc
     5581 aatggatgga aaatgtccac gactgggtta tctctcgtca gctttggtgg ggtcaccaaa
     5641 tccctgcctg gtacaatgct gatggtgaaa tgtatgtcgg cgaagaagct ccagaaggtg
     5701 acggatggac tcaggacgaa gacgtcttgg atacttggtt cagttctgcc ctctggccat
     5761 tttcaaccat gggctggcct gaagtcgact cagaagactt taaacgttat ttcccaactt
     5821 caaccttggt aacaggttac gacatcatct tcttctgggt gtctcgtatg atcttccagt
     5881 cattggaatt cacaggccgt caaccattcc aaaacgtcct tatccacggt cttattcgtg
     5941 acgagcaagg acgcaagatg tctaagtctc tcggtaacgg gattgaccca atggatgtta
     6001 tcgagaaata cggtgccgat gcccttcgat ggttcctttc aaacggttct gcgccaggac
     6061 aagacgtgcg tttctcttat gagaaaatgg atgcttcatg gaactttatt aacaagattt
     6121 ggaacatttc tcgctatatc ctcatgaaca atggaggttt gacgctggat gtggcgcatg
     6181 acaatgtcac aaaagttgca acaggtgagg ctggtaatgt gacggaccgc tggattctcc
     6241 acaatctcaa cgaaaccatt gcaaaagtta ctgaaaactt tgataagttc gagtttggtg
     6301 tggctggtca tatcctttac aacttcattt gggaagaatt tgccaactgg tatgttgagt
     6361 tgaccaagga agttctttac agcgacaatg aagacgataa ggtaattact cgatctgttc
     6421 tcctctatac gctggataaa atccttcgtc tccttcactc aataatgccg ttcgtgacag
     6481 aagaaatctt tggtcagtac gctgaaggct ctatcgtgac agcagcatac ccaactgtca
     6541 acccagtctt tgaggacctt gctgcccaca ctggtgtgga aagtctcaaa gacttgatcc
     6601 gtgccgttcg taatgcgcgt gcggaagtaa atgtagcacc aagcaaacct atcacaatcc
     6661 ttgttaagac tagcgatagt gacttggaag ccttctttaa cagcaatgtc aactacatca
     6721 aacgctttac aaatccagaa catttggaaa tcgcatcaac catccctgca cctgaactgg
     6781 ctatgtcaag tgtcatcaca ggagcagaaa tctacctgcc actggccgat ctcctcaatg
     6841 ttgaagaaga actggcccgt ctcgacaagg aacttgctaa atggcaaaaa gaactggaca
     6901 tggttggtaa gaaactctct aacgaacgct tcgtagccaa cgccaaacca gaagtcgtcc
     6961 aaaaagaatg cgacaaacaa gctgactacc aagccaagta tgatgcgacc gtagcacgta
     7021 ttgatgagat gaagaagttg gtgaaatgat gttaaaaacc taaccctatc gggttgggtt
     7081 ttacatttga taatgttata ataaaaagga gaaactttag gagaatacta tggggaaaat
     7141 tgttgctatt gatttattta acggtgcagg tggcactaca agtggcctta aaaaatcagg
     7201 catagatgtt caagttgctg ttgaaattga ttccgtagct gttaaaactt ataagttaaa
     7261 taatcctgag gtgtctgtaa tagatatgga gtaaataaaa cttcaatttt acaagcttta
     7321 tatggttctc cagccaataa aagtgtgtct gaatattggt ttagtacaaa tgttgatcaa
     7381 atagatgaag tggatgggaa acaatgttta atctattcat attataacgt taaagcttct
     7441 agaaatgtag aagttcttaa aggccgttct ggaacaaaaa aaggattaga ttattgggaa
     7501 ctatatgctc ctcaaaaaca atatgagatg gaaagattac ccaaaaataa atatattgga
     7561 agttcttcga cagacagatg ggatggcatt gaaaaaaatg ttgtcttttg tgattgtaaa
     7621 gaatatgttt ctgcttttga tttatttttc tatcactata attttaaaaa aatttctact
     7681 caacgtagta aacaagattt tataagatta cgatctaaac cagttgctga tattttaaag
     7741 aataatacaa gtagctatac gcgttataaa aaggaaatgg ttatagataa tgttaaagtt
     7801 gatgataaag tttgtgaaat tattagtgaa ataatggatg aatcttatac agacattcaa
     7861 attttaacac ataagttgta ttcaaaaggt gatgatataa aagcttccaa aactatttgg
     7921 atgaaaaaat ctggaaaaga atattccgag gcttttgcag gaacaggtga ggctagaatt
     7981 attttgttag taaatgatat agtaaatgcg caatcaaatt cattaatatt aatagatgaa
     8041 cctgaaatct cacttcatcc gagtgcaatc tataaattta aagagttttt acttcaagag
     8101 tgtttaaata aaaaacatca aattattatc actacacatt ctacacaact tataaaagat
     8161 tttcctagag aagccgtgaa acttttagtg aaaaacggag aaaaggtaga tgttattgaa
     8221 aatattgatt atcaggatgc attttttgaa ttaggtgatg tgtatcattc taggaagatg
     8281 atttatgttg aagatagact agctaaatat attctagagt ttgttatcac tcattcaggt
     8341 agtgagaatc ttaaacagaa tttagtagtg agatatattc ctggtggagc aaatcaaata
     8401 atttgtaata atattttaaa ctcatcgtat ttagattccg ataaccatta tttttggctt
     8461 gatggagatc aaaacactaa tgttagtgaa tcaaataatt taatgaacta tcttgaaaat
     8521 ggtgttgtta tatcagataa aattcctgaa tcagataata aaaatcttga tgatattata
     8581 aaattgataa cgggatgtcc aattaaattt aatgtttcag gtaataaagg gcaaaaaaat
     8641 aatattgaat taattgcgaa acaaagaagc tttatagatt attgggctaa atacgtttct
     8701 tatcttcctt tcccaacccc agagtttttt ttagctaatt tgtgtaacag cgtagatagg
     8761 gaaggttatg atttttctaa agatggcaac ggaaaagaat attttagaaa aaaaactcaa
     8821 gtagcgttag gaatagagaa tataacttct gaagatattt tccaggagca aagaagagct
     8881 gtttcaaaaa tacagccaga atcatcaatg tttcagtgta ttaaagaaaa attagaggca
     8941 ttgttctaat tccttagatt aggtactaca taacaaattt tttaaattca aacccgcacc
     9001 aaaaacaaca ttgcaacttt cttctcaggt aatgtgttgg gatttgaaca gaccaacgag
     9061 ttcatagtgg tgtatgccaa tgactttgat aagtctacgc gtcagactta ttagttcatt
     9121 tatccagata tatctcgata atcatattat ttagtcaaag aacatcccat atgagggcat
     9181 gaaatgctaa gtttagcttg ctcactatta agatgaagta tatatatttc atagaaccta
     9241 ttgtgaagga tttttataat tttcgccaat tccataccta tttcacaatt taccgatgac
     9301 acagaaaaac aattgaaagg atatttctat gcaatcaact tacaacattg acaatccaaa
     9361 cttgtcttat gaagcgaagc gtgatttatg gcggataggt tttggtctgc agaaagttga
     9421 caatctagtg ccatcagcgt atatggaatc tttggctgag aaacagtccc ggggtgaact
     9481 gacttatgag caggtttatg aggatgcaac ggtttatcat cataccgttg atgcgagtac
     9541 ggaggaggca gacttggttt ctttgcgtat cgtagaactc ttgtctcgaa gaggttttag
     9601 attcagtcct gcgactttaa ttgctattca taaggagttg tttcaagata tatttgaacc
     9661 ctctattccg gtggggcaat ttcgtcagac caatatcaca aagaatgaac ctgttttgaa
     9721 tggtgaaagt gttgtgtact ctgattactc catgattcaa atgaccttgg attatgattt
     9781 taatcaggaa aaacaagttg catatgcgac actaactcag gcggatgtgg ttaaacaaat
     9841 ccagcatttt atttcaggaa tctggcagat tcatccattt cgcgaaggaa acactcggac
     9901 agtaacggtc tttttgattc agtatcttcg tgagtttggt tttgatattg ataatatacc
     9961 atttcagcaa cattccaagt attttcgtga tgccttggta ttagataatg caatgtatct
    10021 agatttagac ctcgattttt cataatccaa atactgagta aacattgaat tttaggcaaa
    10081 aatgaagtaa atattctcac aagaaaacgt atatcatcaa agtttggctc tttgtcaatt
    10141 gtagtgggtt gaagaaaagc taagttcgag aaaggacaaa tttcgtcctt tcttttttga
    10201 tgttcagagc gataaaaatc cg
//
LOCUS       DAVDWM010000060        10148 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVDWM010000060 DAVDWM010000000
VERSION     DAVDWM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.060"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(60..293)
                     /locus_tag="VUW49_001903"
     CDS             complement(60..293)
                     /locus_tag="VUW49_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981775.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEDLEQGLERGKVEGRAERK
                     LFTFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(286..402)
                     /locus_tag="VUW49_001904"
     CDS             complement(286..402)
                     /locus_tag="VUW49_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET7981776.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(617..970)
                     /locus_tag="VUW49_001905"
     CDS             complement(617..970)
                     /locus_tag="VUW49_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET7981777.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIHQREGDTH"
     gene            1223..3856
                     /gene="polA"
                     /locus_tag="VUW49_001906"
     CDS             1223..3856
                     /gene="polA"
                     /locus_tag="VUW49_001906"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7981778.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3941..4378
                     /locus_tag="VUW49_001907"
     CDS             3941..4378
                     /locus_tag="VUW49_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7981779.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(4614..5624)
                     /locus_tag="VUW49_001908"
     CDS             complement(4614..5624)
                     /locus_tag="VUW49_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET7981780.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            5773..6942
                     /locus_tag="VUW49_001909"
     CDS             5773..6942
                     /locus_tag="VUW49_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7981781.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAGKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            6939..7709
                     /gene="recO"
                     /locus_tag="VUW49_001910"
     CDS             6939..7709
                     /gene="recO"
                     /locus_tag="VUW49_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7981782.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            7706..8698
                     /gene="plsX"
                     /locus_tag="VUW49_001911"
     CDS             7706..8698
                     /gene="plsX"
                     /locus_tag="VUW49_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7981783.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            8704..8937
                     /locus_tag="VUW49_001912"
     CDS             8704..8937
                     /locus_tag="VUW49_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7981784.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            8983..9273
                     /locus_tag="VUW49_001913"
                     /pseudo
     CDS             8983..9273
                     /locus_tag="VUW49_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9475..9705
                     /gene="blpU"
                     /locus_tag="VUW49_001914"
     CDS             9475..9705
                     /gene="blpU"
                     /locus_tag="VUW49_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET7981785.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
BASE COUNT         2958 a         1830 c         2484 g         2876 t
ORIGIN      
        1 aaaataggct ccataatatc cataggggat ttactcacta tggatattat ggagccattt
       61 tacaacagtg cctcaaattc agatactgac atacctaatt gctggctggc aacctcagaa
      121 gtcagaagac cttggcgtac tatgtctagg aaggtaaaaa gtttcctttc tgcccttcct
      181 tcaactttcc cacgctctag tccttgttca aggtcttcag ccctagcagt ttccaaggca
      241 tagtcctgtg ctaacaagac ttgttcttct cgcatatgta gttgactaaa cattttcctg
      301 tcctcctcgg accagctctt gtagtccagc agttgatttg cttgggtaat ggctcgttgc
      361 ggttgctggg taaagggctt gttgccgaaa aactccaacc atggcttgcg aaccttgtct
      421 ttgctggttt ctctgtattt tttaattcca agaatgccat cttaaccaga tggttttcct
      481 gtccattgtt ggtaatcgcc aatacctcac ctgttgtgtc ttcgcgcata ctaaagctat
      541 gaaaagccag gtcatctgag aaataattac tatccacgat agcaatgacg taaacaggag
      601 cgatgtgttt gtagatctag tgagtatcac cttctcgctg atgaattttt tcaagatttt
      661 gattaacctg actgcacagg taagcccaca agtgattgat gaaaaaattc tgatgatgga
      721 cttgaatctc aataattact tgagtaccgt tatccaactc cgccaagacg tctatactgg
      781 tataaaaatc ctgcaccgag taaggcatgg agagtaatac atgaatatcg cttccctcca
      841 aaatggtcac attttttgct ggcaagtcca gcatatcgcg aataaattga caagtgattt
      901 ctggattgct gaatattttc ttagcaacca agtcattggt cgggctaatt cccagatgtc
      961 tgagaatcat ccttttcctc cttctatagt gaattgaaat aagatgtgaa caactctatc
     1021 aggaaagtca aattaattta tagaaatatt ttagcagtca aggtgtacta ttatagattc
     1081 aatatactat attatagcac atttttaaat ctaggtttac taaattctct ggctctatct
     1141 atttattcgg aaaaagtttg aaaaatactt gctctagctc ttcccaatgg tattttttga
     1201 ttctttcctt tataatgggt gtatggataa gaaaaaatta ttattgattg atgggtcttc
     1261 tgtagctttt cgggcgtttt ttgcgctgta tcagcagttg gaccgtttta agaatgcggc
     1321 tggtttgcat accaatgcga tttatggttt tcagttgatg ttgagtcatt tgttggagcg
     1381 ggttgagccg agtcatattt tggtggcttt tgatgcggga aagacgacct tccggacaga
     1441 gatgtatgcg gactataagg gtggtcgggc caagactcct gatgagtttc gtgagcaatt
     1501 tcctttcatt cgtgagttgc tggatcatat ggggattcgt cactatgagc tggctcagta
     1561 tgaggcggat gacatcattg ggacgctgga taagctagca gagcaggatg gttttgatat
     1621 taccattgtc agtggggaca aggatttgat tcagctgacg gatgagcata cggtggttga
     1681 aatttccaag aaaggtgtgg ctgagtttga ggcctttacg ccagattacc tcatggaaga
     1741 aatgggcctc acaccagctc agtttatcga tctcaaggcg ctcatgggtg ataagtcgga
     1801 taatatccct ggggtgacca aagtcggtga aaagacgggt attaagctct tgctggagca
     1861 tggttcgctt gaggggattt atgaaaatat tgatggaatg aagacttcta agatgaagga
     1921 aaatctcatc aatgacaagg aacaggcctt tttgtctaaa acactagcga ccattgatac
     1981 caaggcaccg attgcgattg gtttagagga cttggtctat agtggtccag atgttgaaaa
     2041 tcttgggaaa ttttacgatg agatgggatt caaacagctc aagcaggctt taaatgtgtc
     2101 gtcagctgat gtggctgagg gtttggattt tactattgtt gaccaaatca gtcaagatat
     2161 gctgagtgaa gagtctatct tccactttga gctttttggt gagaattacc atacggataa
     2221 tttggttgga tttgcctggt cttgtgggga taagctctat gccacagaca agcttgagct
     2281 gttgcaagac ccgattttca aggatttctt agaaaaaaca tctctgagag tttatgactt
     2341 taagaaggtt aaagttcttt tgcaacgttt tggtgtggat ttgcaggcgc ctgcttttga
     2401 catccgtttg gctaaatacc tcctttcgac tgtggaggac aatgaaattg cgaccatcgc
     2461 tagtctttat ggtcagactt acttggttga tgatgaaact ttctacggta agggtgttaa
     2521 aaaggccatt cctgaacgtg agaaattctt ggaacactta gcttgtaaac ttgctgtttt
     2581 ggtagaaaca gagcctattt tacttgaaaa actcagcgaa aatgggcaat tagagcttct
     2641 ttatgatatg gagcaacctc tggcttttgt ccttgccaag atggaaattg ctgggattat
     2701 ggtcaagaaa gagaccttgc ttgagatgca ggctgaaaat gagcttgtca ttgaaaaact
     2761 gactcaagag atttacgagc tggctggtga ggagtttaat gtcaactcgc ctaagcagtt
     2821 gggcgtgctt ctctttgaga aattgggact tcctctagaa tacactaaga aaaccaagac
     2881 aggttattcg acagcagtgg atgttttgga gcgtctcgct cctattgctc cgattgttaa
     2941 gaaaatcctg gattaccgtc aaattgctaa gattcaatct acttatgtaa ttggcttgca
     3001 ggactggatt ttggctgatg gaaagattca tactcgctat atgcaggatt tgacccagac
     3061 cgggcgtttg tctagtgtgg atccaaactt gcaaaatatt cctgcccgat tggaacaagg
     3121 gcgcttgatt cggaaggctt ttgtgccaga gtgggaggat agtgtgctac tcagctctga
     3181 ctattcacag attgaattgc gcgttttggc gcatatttct aaggatgagc acttgattaa
     3241 ggccttccaa gagggggcag atatccatac ttcgacagcc atgcgggtct ttggcattga
     3301 gcgtcctgat aatgtgactg caaatgaccg tcgcaatgcc aaggcagtta actttggagt
     3361 ggtttatggg atttcagact ttggcttgtc taataatttg ggaattagtc gtaaggaagc
     3421 caaagcctac attgatacct actttgaacg ttttccaggt attaaaaact acatggatga
     3481 agtggtgcgg gaggcgcgtg ataagggcta tgtagagacc ctctttaagc gtcgccgtga
     3541 gttgccagat atcaattcgc gcaacttcaa tattcgtggt tttgcggagc gaactgctat
     3601 caactcacct atccagggtt cggcagcaga tattctcaag attgccatga ttcagctgga
     3661 taaagccttg gttgcaggtg gttatcagac taagatgctg ttacaagtgc acgatgaaat
     3721 cgtccttgaa gtgcctaaat ctgaattggt agagatgaaa aaattggtga aacaaaccat
     3781 ggaagaagcc attcaactca gtgttcctct tatcgcagat gagaatgaag gggcaacctg
     3841 gtacgaggct aaataaaaag ggggctagtc ctcctttttt gtagtagaat tctgcaaacc
     3901 ttttcaaaat gtgctatact gatgaaaaag gaggatttct atgagtcaag aatttatcaa
     3961 tccaagtgat ggcgtgattc gtcagtatct cgcaacgagt aaaacccttg ctgtggtggg
     4021 tttgtctgac cgtgaagaaa caactagcaa tcgagtaaca aaggaaatgc aggctcgggg
     4081 ctataaaatc atcccagtca atcccaaggc ggcaggtggc gaaatcttgg gtgaaaaggc
     4141 ttatgctagc ctcgctgaaa ttccttttcc tgtagatatt gtcaatgttt atcgtcgcag
     4201 tgaatttctg cccgatgtgg cgcgtgattt tctcaaagct gatgccaaga ttttttgggc
     4261 acagctagga cttgaaagtt tagaagcgaa agagattttg cgtgatggtg gatgtgatga
     4321 tatcgtgatg aatcgctgta tcaagagaga acatactcgc ttgattgagg aagcataaga
     4381 aaaaggtagc tgatgggcta ccttttgtgt tatactcaat gaaaatcaaa gagcaaacta
     4441 gcaaactagg aaactagccg cgggctgctc aaaacactgt tttgaggttg tagatacgac
     4501 tgacgaagtc agctcaaaac accgttttga ggttgcagat agaactgacg aagtcagtaa
     4561 catatatacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga gtattagaaa
     4621 atgccgataa gggtctgcat accaagagtg gtgaggatga tggcaatcca gcagatggct
     4681 ccaagaagaa tggattttcc actggatttg accatagcga ccagattggt tttgagaccg
     4741 atagcactca tggccatgac aatgaggaat ttagagagtt gtttgagagg ggtaaagaaa
     4801 ctactagaca caccgagaga ggtcagaagg gtggttagga gcgatgcaag gataaagtaa
     4861 aggataaaaa gtgggaagac ttttttcagt tgtaggcttt gcttgttttc ttgttggcga
     4921 ctttgccagt aggatagaaa gagcgtgata gggataatgg ccaaagtacg tgtgagttta
     4981 acaatggttg cagactcgag ggtattgctt tggtaaagac tgtcccaagc gctggcggca
     5041 gctgttacag aggaagtgtc gttgaccgca gtccctgcaa agagggcgaa gccttcattg
     5101 gatagatgaa gccaggtgcc gagggttgga aagatgagcg cagccaagac attgaagaaa
     5161 aagataacgg aaatggcttg ggctacttcc ttttccttag catggataac aggcgctgtc
     5221 gctgcaatgg cagaaccccc acagatagaa gaacctactc caaccaaggt agccagtttt
     5281 gtatccagtg caaagaaacg ctggaagagg taggcaataa tcagagctat tgagatagtg
     5341 gacaggatga cagggagtga agattggcca actgcgaaga cctgcgagat attgagacca
     5401 aaaccaagca agacaacggc atattggagc aacttcttgg aactaaaggt caaaccagca
     5461 tccagttgtt tataggacga gagaaaggga tgtaggagca tgcctatgaa aatggcaaaa
     5521 acgggcgcgc caatcacagg gaagaatcct cctaagtacc aagataggat ggaaatgaga
     5581 aggcaggcca agatacctgc tccatttttt gatagaaatg acataaaaac ctccaaataa
     5641 aatctattac cattatagac ctataaacag gaaaagtaaa atagaaagtg gaaagctatt
     5701 ctctaatgta tttttgcggt cggggggctt ttgtagtata atagagatac gttttgaaag
     5761 taggaggtat ctatggactt aactaagcgc tttaataaac agttagataa aattcaagtt
     5821 tcgttgattc gtcagtttga ccaggctatt tcggagattc ctggggtctt gcgtttgacc
     5881 ttgggggaac ctgattttac aacgccagac catgtcaagg aggcgggcaa gcgagcgatt
     5941 gatcagaacc aatcctacta tacagggatg agtggtctgc tgactctacg tcaggcagcc
     6001 agtgactttg ttaaggaaaa gtaccaactg gactatgctc ctgaaaatga aatcttggtt
     6061 acaattgggg cgacagaggc tttatctgcg actttgacgg ctattttgga agagggagac
     6121 aaggtacttt tgccagctcc tgcttatcca ggctatgaac cgattgttaa cttagttggg
     6181 gcagaaattg ttgagattga tacgactgaa aatggttttg tcttgactcc tgagatgttg
     6241 gagaaggcca ttttggagca gggtgataag ctcaaggcag ttattctcaa ctatccagcc
     6301 aatccgacag gaattaccta cagtcgagag cagttagagg ccttggcagc tgttttacgc
     6361 aagtacgaaa tttttgttgt ctgtgatgag gtttactcag aattgaccta cacaggcgaa
     6421 gcccatgtgt ctctaggaac gatgttgaga gaccaggcta ttattatcaa tggtttgtct
     6481 aaatcgcatg ccatgacagg ttggcgtttg gggctgattt ttgctcctgc gaccttcaca
     6541 gcccagttaa tcaagagtca ccagtacttg gtcactgccg caaataccat ggcgcaacat
     6601 gctgcggtag aagccttgac ggctggtaaa aacgatgcgg agcccatgaa gaaggaatat
     6661 atccaacgtc gggactatat catcgaaaaa atgactgctc ttggttttga gattatcaaa
     6721 ccagacggtg ccttctatat ttttgctaaa attccagcgg gctacaatca agactccttt
     6781 gcttttctga aggattttgc tcagaagaag gccgttgcct ttatccctgg tgcagccttt
     6841 ggacgttacg gggaaggcta cgtccgccta tcttatgcag ccagcatgga gactatcaaa
     6901 gaagccatga aacgacttga ggagtacatg agagaagcat gattcagtct atcacgagtc
     6961 aaggcttggt gctttacaat cgcaattttc gtgaggatga caagctcgtc aaaattttta
     7021 cagagcaggt tggcaaacgc atgttttttg tcaaacacgc tggtcagtct aagctggcgc
     7081 ctgttattca gcccttggtg ctggcacgat ttctcttgcg aatcaatgat gacggactca
     7141 gttacatcga agactatcat gaggtcatga cttttcccaa gattaatagt gacctctttg
     7201 tcatggccta tgcgacctat gtggcagctc ttgcagatgc tagtttgcag gacaatcagc
     7261 aggatgctcc cttgtttgct tttttgcaaa agactttgga gttgatggaa gcaggcttgg
     7321 attatcaggt tttgaccaat atttttgaaa ttcaaatttt gactcgattt ggaatcagcc
     7381 tcaattttaa tgagtgtgtc ttctgccatc gggttggtca ggcttttgac ttttctttca
     7441 aatatggagc ctgcctctgt ccagagcatt atcatgagga taagagacgt tgtcatctca
     7501 atcccaatat cccctatctg ctcaatcaat ttcaagctat tgattttgag actttggaga
     7561 ccatttcgct caagcctgga atcaagcaag agctacgcca atttatggat caattatatg
     7621 aagagtacgt tgggattcac ctaaaatcaa agaaatttat tgattcccta gcagactggg
     7681 gacaattact aaaagaggaa aagaaatgaa aaaaatcgca gtagatgcca tggggggcga
     7741 ttacgcacct caggccattg ttgagggtgt caatcaagcc ctatctgact tttcagatat
     7801 cgaggttcaa ctttacggag atgaagctaa aatcaagcaa tatctgacag cgacagagcg
     7861 cgtcagcatt atccatacgg atgagaagat tgattcggat gatgaaccta cgagagctat
     7921 taggaataag aaaaatgcca gtatggtatt ggcagccaag gctgtcaaag atggtgaagc
     7981 agacgctgtc ctttcggctg ggaatacagg tgccttgttg gctgctggat tcttcatcgt
     8041 gggtcgtatc aagaatatcg accgtcctgg gcttatgtcg actttgccga ctgtagatgg
     8101 gaagggattt gacatgctag acctcggtgc caatgcggaa aatacagccc agcacctcca
     8161 tcaatacgcg gttctaggat ccttctatgc taaaaatgtt cgtggcattg cgcaaccacg
     8221 tgttggcttg ctcaataacg gaacagagag tagcaagggc gatccgcttc gtaaggaaac
     8281 ttatgaatta ctagtggctg atgaaagttt gaactttatc ggaaatgtgg aagcgcgtga
     8341 tttgatgaat ggcgttgcag atgttgttgt ggcagatggt ttcacgggaa acgctgtgct
     8401 caaatccatc gaagggacag ctatgggaat catgggcttg ctcaagacag ctattacagg
     8461 tggtggtctt cgagcgaaac taggtgccct ccttctcaag gacagcctca gtggtttgaa
     8521 aaaacagctc aattattcag atgttggtgg agcggtcttg tttggtgtta aggcacctgt
     8581 tgtcaagact catggctcaa gcgatgccaa ggctgtttat agtacgattc gtcagatccg
     8641 taccatgcta gaaacagacg tggttgccca gactgcgcgt gaattttcag gagaataaaa
     8701 gagatgacag aaaaagaaat ttttgaccgt attgtgacca ttatccaaga gcgacaggga
     8761 gaggactttg tcgtgacaga atccttgagt ctgaaagacg atttggatgc ggattctgtt
     8821 gacttgatgg agtttatctt gactctggaa gatgaattta gtatcgaaat cagcgatgaa
     8881 gaaattgacc aactccaaaa cgtaggagat gtggttaaaa tcattcaagg aaaatagcaa
     8941 tcggagttcc aagtcaacgg aagtagatgg tttttagaaa tgagaaatat cggacaagct
     9001 ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc tcaagcacaa
     9061 actccacgta aatccagcaa actcaagccg ctaacagttg aagataaagc ctgtaatcat
     9121 gcgctatcta aggagagaag caaggttgag aatatctttg ccaaagtaaa aacgtttaaa
     9181 atgttttcaa caacctatcg aaatcatcgt aaacgcttcg gattacgaat gaatttgatt
     9241 gctggtatta tcaatcatga actaggattc tagttttgca ggaagtctaa tagtaaaaag
     9301 tgattagaaa acatcttttt taaaaataga gatgattttg aaacaaaaaa gctaattcaa
     9361 gacgtttcga tgccaattca agatttggat gaaaaaaatt aatagatact gttatactaa
     9421 acttgtcaag tttgtaacaa gacaaatatt aaaaataaaa aagaggtatt cgttatgaat
     9481 acaaaaaaga tgtcacaatt tgaaattatg gatactgaga tgcttgcttg cgttgaaggt
     9541 ggcggatgca attggggaga ttttgccaaa gcaggtgttg gaggaggagc agtacgaggt
     9601 cttcagctag gaattaaaac aagaacatgg caaggtgcag caactggtgc tgcgggagga
     9661 gctatacttg gaggtgtggc ctatgcagcg acatgttggt ggtaattatg gattttaaaa
     9721 gttttattat tggtttagta gttggtatat ttggtcctta tatggatgat ttaattagaa
     9781 aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa taatcaaaac
     9841 tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt cttatagtaa
     9901 cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct attctgacta
     9961 ggaatagatc ataccagagg tagcttagaa atagcagaga cattagaaat tgaagtaata
    10021 aataggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg cctagggtga
    10081 cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcaggact cttgttctgc
    10141 ctattttt
//
LOCUS       DAVDWM010000061         9901 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVDWM010000061 DAVDWM010000000
VERSION     DAVDWM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.061"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            190..285
                     /locus_tag="VUW49_001915"
                     /pseudo
     CDS             190..285
                     /locus_tag="VUW49_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(538..1158)
                     /locus_tag="VUW49_001916"
     CDS             complement(538..1158)
                     /locus_tag="VUW49_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7981786.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTPNTWNDTSYQS"
     gene            complement(1158..1640)
                     /locus_tag="VUW49_001917"
     CDS             complement(1158..1640)
                     /locus_tag="VUW49_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET7981787.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1640..2482)
                     /locus_tag="VUW49_001918"
     CDS             complement(1640..2482)
                     /locus_tag="VUW49_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET7981788.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2577..3377)
                     /locus_tag="VUW49_001919"
     CDS             complement(2577..3377)
                     /locus_tag="VUW49_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET7981789.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTSKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3531..4910
                     /gene="glmU"
                     /locus_tag="VUW49_001920"
     CDS             3531..4910
                     /gene="glmU"
                     /locus_tag="VUW49_001920"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7981790.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4920..5465
                     /locus_tag="VUW49_001921"
     CDS             4920..5465
                     /locus_tag="VUW49_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7981791.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5477..5791
                     /locus_tag="VUW49_001922"
     CDS             5477..5791
                     /locus_tag="VUW49_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET7981792.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5808..6500
                     /locus_tag="VUW49_001923"
     CDS             5808..6500
                     /locus_tag="VUW49_001923"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET7981793.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQSQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6577..7068
                     /gene="rocS"
                     /locus_tag="VUW49_001924"
     CDS             6577..7068
                     /gene="rocS"
                     /locus_tag="VUW49_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET7981794.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7166..7756
                     /locus_tag="VUW49_001925"
     CDS             7166..7756
                     /locus_tag="VUW49_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7981795.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPRKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7765..9528
                     /locus_tag="VUW49_001926"
     CDS             7765..9528
                     /locus_tag="VUW49_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7981796.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            9742..>9901
                     /locus_tag="VUW49_001927"
     CDS             9742..>9901
                     /locus_tag="VUW49_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7981797.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVK"
BASE COUNT         3085 a         1716 c         2102 g         2998 t
ORIGIN      
        1 aagacttttt gaccagcctc atttttttaa cttgatactc agtgaaaagc aaagattaaa
       61 ctaggaagct agctgtaggc tgctcaaaga acagctttga ggttgtagat aaaacttgtg
      121 aggtcaccaa catatataat gtgaagctga cgtggtttga atagatttta gaagagtatg
      181 agtctggaag ttttaatgga taatgcaaga ttccatagaa tgggtaagct agagttctta
      241 tgtgaagagt ttgggcataa acttttacct tttcctccct actcatctta gtatagaaaa
      301 gtgaatctga aatagtacat aactgcttct aaaacattct tataaattga tttaaattct
      361 caaatcatat tattcagttc ttatttcatt ttgttctaca atcctgttga gaagacacgt
      421 gttcatatca aaaaggtatt ggcaagttgc aatacctttt tacgaggctc tgttgtctta
      481 tttttgtttc aactgactat atctcctatg gttctagttc agaaggctag gctataatta
      541 tgattgataa gaagtatcat tccaagtatt gggagtgaat gtttcaaaat catgggtttc
      601 tataatggtc aggctggcat ttgctagacc gccatcttta cgaagaagtg gttctttata
      661 gcctaggaga gtacgaagac tggcagtaag attggcgccg tgtccgacaa ttagaatacg
      721 ttcagctgga ctatctttta atgatttgat aaattggatg gtccgttgag ttgtactata
      781 gagggattcg gctccgaaca ttcgagtgtc aaattgagca agatttgaac gaaaagcctg
      841 gatttgttgc gggtaaatag cttccaaggt tgcaattttc aaaccttcta acttcccaag
      901 ttgccattca cggagattag gaacgatttc taaagaacag ggggtataga gttgactttg
      961 gataatctca gcagatttga ccgctcgagg taaatcactt gaataaatct gatcaaaagg
     1021 aatttccttg agatactgac caagtcgttt tagggtttca atggattcag gaagaagagg
     1081 agaatcacca ctagcacctt gaaaacgacc ttcttggttc cagagggtac gaccgtggcg
     1141 gacaaagtag agtttcatta cttgatgtcc tccaaaatat ctacaaagtc tgcctttaca
     1201 aagctagcca agtcttgtgg cgcgacgata atgctgtgtc cgacttcgcc tgcagagaca
     1261 atcatttgat ccaaatctag agcaatttta tcgataaaaa tgggataatt gtgtttctga
     1321 cgaattccga caggattatt ggctccatga atgtaaccag ttgttttttc taagtccttt
     1381 tgtggaatca tgctcacttt tttattgcca gaaattttag ctagtttctt ttcagacaag
     1441 tgctgagtga tagggacaat tccgataatc ggtccggtct tgtctcccaa aagcgccaag
     1501 gttttgaaaa tctgatctcg ttcataacct tgaggaagct ctccttctag ggcattgatt
     1561 tgaatcccct gatgagggat agctgcttta gataggattt gttccaccaa tgtttttttg
     1621 attttaactt tttttgccat tatttatatt tatcctccaa ttgactcatc caaataccaa
     1681 gccagattcc cagcgcaaag aagaaggcga tgatgacata accgacaagt gaaagtcctg
     1741 tgtattggat actttcagcg tttcctgcat ttggaattaa gatcaaaagg gtacttgata
     1801 ggacgatacc gatgatgaaa tgatagacac gtgagtggta gttgtttaag gcataatcca
     1861 tcaattttga aaaaacgatg agagttgcac ctgcaccaat tccaatcgga aagaaggttc
     1921 ccaagaaatc aaaagtttta aaaccagtca acataggagc atagagtccc aaaatcaaaa
     1981 gtaaatttga tgggctgagg ccaggaacca agacgccaag ggccaatagt gcgcctgcta
     2041 ggacgaagtt aagaaagctg gcgcttaagg ttccaacgac aaaatttaag gcatagagtc
     2101 ctaatccaga aatgataaag gttgtccata accaagctaa atcaatcttg tctcggtcag
     2161 attctcgagt tgattctttg aggaggctag gaactgtacc gataatagct cccgcaaagc
     2221 tccataatac aaaaacctga taattttcaa gcaggtattc aatcgggtag gaaaataagc
     2281 cgattcccag aagcataccg atggcaactg gaataaagta caaaacattt tctttaaagt
     2341 ctttaaaggg atgggccaga aagccaatca ttcgttcata gattcctaag attgctgcta
     2401 gaacccctcc ggaaattccc ggtaggataa atccaagagc aattacaatc cctttaataa
     2461 cgcgtgctaa ccatgagagc atatatttcc tccaactatt tctatttcaa aaaatcctta
     2521 ctatattgta tcacaatcag acacaaaaag aaaaagcaaa tgataaacaa atgcttttaa
     2581 ggttttaaaa aaagttttcg aaaggtttct tctttatttt ttaagggaga gataacgttg
     2641 atatctaaat cgtggtcaaa gccggcaatt tttcctttag atgtgtattg gtgaatatca
     2701 taatctaaat cagttttagg actgctctcc aaaaatcctg agtctgagcc gtaggacgga
     2761 atccaaacag aggtaaactt gcttgtatca atactgtgtt cttccatgaa gtagacacca
     2821 acgtagatgc cgatgttttt agcacctagt gatgctagtt ttgctcgaaa gttttcgacc
     2881 ccttcgttca tattagacat ggttttgtct tccacgtcaa gccaatagta actagggctg
     2941 taaggagagg cagcattgta gaaaacttcg gcagcctttt ccatttcttg gacacttttt
     3001 ccagctacat aagcgtagac agcaactggg acattccgct tttgaagttc agtgatatga
     3061 ctcttatagg ccttgtctat tccattgata aatgaagcat cattttcttt tgtcgtttga
     3121 gcaccactgt gaacacgaac aatagcacct gaaatatttt gtgagagggc atcgtagttg
     3181 atttcctcag gacgctgcca gccagagagg tcaataatcg gtttgtctaa gtgtttcaaa
     3241 gcctgtgctt caatctgtgc tatattggat tttgttttaa acgattggct gtcattaagt
     3301 gggcgattga tgattaaaat gaacatcata atcccaaaaa aactaaataa aataagtgga
     3361 tgaatttgtt ttctcatatc ttataagtct accctaaaaa tcaaaaaaaa tcaaaaaaaa
     3421 tcaaaaaaat gggttaagga agagacttta gagcattttt tcattcaaga gtgcggaatg
     3481 atttgaaata tggtataata aaagggaatt tctacagaaa agagaagatt atgtcaaatt
     3541 ttgccattat tttagcagcg ggtaaaggga ctcgcatgaa atctgatttg ccaaaagttt
     3601 tgcacaaggt tgcgggtatt tctatgttgg aacatgtttt ccgtagtgtg ggagctatcc
     3661 aacctgaaaa gacagtaaca gttgtaggac acaaggcaga attggttgag gaggtcttgg
     3721 ctggacagac agaatttgtg actcaatctg aacagttggg aactggtcat gcagttatga
     3781 tgacagaacc tatcttagaa ggtttgtcag gacacacctt ggtcattgca ggagatactc
     3841 ctttaatcac tggtgaaagc ttgaaaaact tgattgattt ccatatcaat cataaaaatg
     3901 tggccactat cttgactgct gaaacggata atccttttgg ctatggacga attgttcgta
     3961 atgacaatgc tgaggttctt cgtattgttg agcagaagga tgctacagat tttgaaaagc
     4021 aaatcaagga aatcaacact ggaacatacg tctttgacaa cgagcgtttg tttgaggctt
     4081 tgaaaaatat caataccaat aacgctcaag gcgaatacta tattacagac gtcattggta
     4141 ttttccgtga aactggtgaa aaagttggcg cttatacttt gaaagatttt gatgaaagtc
     4201 ttggggtaaa tgaccgtgtg gcgcttgcga cagctgagtc agttatgcgt cgtcgcatca
     4261 atcataaaca catggtcaac ggtgttagct ttgtcaatcc agaagcaact tatatcgata
     4321 ttgatgttga gattgctccg gaagttcaaa tcgaagccaa tgttaccttg aaagggcaaa
     4381 cgaaaattgg tgctgagact gttttgacaa acggtactta tgtagtggac agcactatcg
     4441 gagcaggagc ggtcattacc aattctatga ttgaggaaag tagtgttgca gacggtgtga
     4501 cagtcggtcc ttatgctcac attcgtccaa attcaagtct gggtgcccaa gttcatattg
     4561 gtaactttgt tgaggtgaaa ggatcttcaa tcggtgagaa taccaaggct ggtcatttga
     4621 cttatatcgg aaactgtgaa gtgggaagca acgttaattt cggtgctgga actattacag
     4681 tcaattatga cggcaaaaac aaatacaaga cagtcattgg agacaatgtc tttgttggtt
     4741 caaattcaac cattattgca ccagtagaac ttggtgacaa ttccctcgtt ggtgctggtt
     4801 caactattac taaagacgtg ccagcagatg ctattgctat tggtcgcggt cgtcagatca
     4861 ataaagacga atatgcaaca cgtcttcctc atcatcctaa gaaccagtag gagcctatca
     4921 tggagtttga agaaaaaacg cttagccgaa aagaaatcta tcaaggacca atatttaaac
     4981 tggtccaaga tcaggttgaa ttaccagaag gcaagggaac tgcccaacgg gatttgattt
     5041 tccacaatgg ggctgtctgt gttttagcag taacggatga acaaaaactt atcttggtca
     5101 agcagtaccg caaagctatc gaggctgtct cttacgaaat tccagccgga aaattggaag
     5161 taggagaaaa cacagcccct gtggcagctg cccttcgtga attagaggaa gaaacagcct
     5221 atacagggaa attagaactc ttgtacgatt tttattcagc tattggcttt tgtaatgaga
     5281 agttaaaact atatttagca agcgatttga caaaagtgga aaatccgcgt ccgcaggatg
     5341 aggatgaaac cttggaagtc cttgaagtga gcttagaaga agcgaaagaa ttaatccaat
     5401 caggtcatat ttgtgatgcc aagacaatta tggctgttca gtattgggag ttgcagaaaa
     5461 aatagaggag gtcagtatgg gtaaatcttt attaacggat gaaatgattg aaagagctaa
     5521 tagaggcgaa aaaatttcag gtcctccttt gctagatgat aatgaggaga ctaagatttt
     5581 accaacctct tcttcccgtt ttggttatgc caatcctaag aatcatggtt ttagccagga
     5641 aaccttgaag attcaggtcg aaccatctat tcataaaagc cgtcgcattg aaaataccaa
     5701 gagaaatgtc ttcaattcta agttgaataa aatcttattc gcggtcatct ttctcttgat
     5761 tttgcttgtt ttagcaatga aacttttgta atagaaaagg aattgaaatg aaaataggaa
     5821 ttattgctgc tatgccagaa gaactggctt atctggtcca gcatttagat aatgcccagg
     5881 agcaagttgt ttttgggaat acctatcata caggaaccat tgcttctcat gaagtcgttc
     5941 ttgtagaaag tggaattggt aaggtcatgt ctgctatgag tgtggcgatt ttggctgatc
     6001 atttccaggt ggatgccctt attaatacgg gttcagctgg ggcagtagca gaaggtatcg
     6061 ctgttgggga tgtcgtgatt gctgacaaat tagcctatca tgacgtggat gtcacagctt
     6121 ttggctatgc ttatggacaa atggcgcaac aaccgcttta tttcgaatca gacaaaacct
     6181 ttgttgctca aagccaaaag agtttatctc aattggacca aaactggcat cttggtttga
     6241 ttgctacagg agatagtttt gttgcaggaa atgacaagat agaagcgatt aagtcccatt
     6301 tcccagaagt tttagccgtg gagatggagg gggcagctat tgctcaagca gcgcatgccc
     6361 tcaatctccc agtcttagtc atccgagcta tgagtgacaa tgccaaccat gaagcaaaca
     6421 tcttttttga tgagtttatt atcgaagctg gacgtcgctc tgcccaagtc ttgttgacct
     6481 ttttgaaggc tttagattaa gcggaaattt gacagttttt ctagcttatg ataagattta
     6541 agtaaagaaa agctagaaaa cgtttcagag gatattatga gtattgaaat gaccgtcagt
     6601 gagattgcag aggtcttagg attatctcgc caagcaatca ataaccgtgt caaagaatta
     6661 ccagaagaag acacagataa aaatgacaaa ggggtaacag ttgttaccag aagtggcttg
     6721 attaagctag aagaaatcta taaaaaaacg atttttgaag atgagcctgt cagtgaagat
     6781 gtcaaacaac gtgaactgat ggagattcta gtggatgaga agaatgcaga aattcttcgt
     6841 ctttatgaac aattaaaagc caaggatcgt cagttatcag aaaaagacga gcagatgcgt
     6901 atcaaagacc gtcagattgc tgagaaagac aaacaattag atcagcaaca acaattgacc
     6961 cttcaagcca tgaaggatca agaaaatctt aagctagagc tggaccaagc aaaagaagaa
     7021 gtccaatcca ctaaaaaagg cttttttgct cgtttatttg gaggataatc aaggagctgt
     7081 ttaggttaaa tgctaacctg atggcttctt ttgtttgtga atagtatagt tgattagaac
     7141 agagacagta gaggagaggt acagaatgga acgattagaa gattacattg cttttgattt
     7201 agaatttaat cagcacgaag gacaaacaca cttgattcaa gtatcggcag tgcgttttag
     7261 agatggtcgg gaaatcgatg cctatgattc ctatgttcat accagcgtcc ctcgaaaaag
     7321 ctttatcaat ggtttgaccg ggattacagc tgagacctta aaagatgctc ctccagttga
     7381 gaaagtctta gaagaattcc aagagtttgt gggttctttg ctgatgatag gctacaatgc
     7441 tgctaaaagt gatttaccta ttttagcaga gcatggttta gactatagcc aacagtacaa
     7501 ggtggatttg tatgatgaag cttttgaacg tcggagttca gatttacatg gtattgccaa
     7561 tctcaaattg caaactgttg cctcatttct tggatttcaa ggtcagtctc ataatagttt
     7621 agaggatgct cggatgacgg cgcgtgttta cgaatccttc ttagagtcgg atcaagctag
     7681 ggaattgtta gagacagaaa gtagcttatc aaacaatcct tttggtggct tggacttgtc
     7741 tcaattattt gactaggagt gcctatgaaa cctgaaaaac ctaaaaaatt gggtcttaaa
     7801 cagatttacc tcaatcttga taaaatcctc tttctatttt tcttgatttt tatgatagtg
     7861 gagtatttgt gggtaccatt caattcatgg ttggctagct tcttattaga tcagacaggt
     7921 tatcttttta tttcctataa caatgtctta gccatcctta ctcattctcc attgattagt
     7981 ctagcttttt tagccttgat tgtaattaac cttctggttg cttattttca aataggactc
     8041 ctctttattg gtgctcgtca tctcctctat aatgaaaagc gaaggctaat tgagtatatc
     8101 cgcaaggttt tccatgaaag ttttggattt atgaaaaagc taacttgcgc taaagccttg
     8161 tttatctttt tctatatagc tatgctgttt ccttttatac ggaaaatgct gaagatttat
     8221 tatcttaata aaatcgtcat tccagagttt atccaagctt atttagaaga tagatattgg
     8281 atgtggtggc tgagcatcct acttctatct cttatctttc tctatgtctc tgtcagattg
     8341 atgtttgccc ttccgaagat tgtatatgat cagctgactg ttcgagaagc agtgatgttt
     8401 agcttggaga aaaccaagaa gagagttact ttttatgctt ggaatttatt ttacatcttg
     8461 ctcaaagcac atttattatt ttatcttcct ttgattcctt tattattggc tcaaactttg
     8521 gtagatgata tcactcagag agagtctctg attcttggta ttttgaattt tgtcgtgatt
     8581 aaaaatctct attatatggc tctgacttat tttttggtta aatttgtttc atttttgaca
     8641 ggaaaggagc tggatatgct tcctaggaga gaaaaagatc acatcgtgcg atggggtgtc
     8701 atgacttgtg ccagtctttt ctttgcctta gaaggttata tttatctgga ggctcccatg
     8761 gttcatctac ctcaacttat ttctcaccga ggggtttcca atgcaaatgg gattcaaaat
     8821 acagtagagt ccttggaaac tacagcacaa ctcaaaccag acttggtgga gacggacgtg
     8881 caggaaacaa aagatgggca gtttgtcatg atgcatgatg ctaacttgaa aaatctagca
     8941 ggtatcaata aaagtcctca agacttaaac ttggaggagc ttaaagggat tgatattttt
     9001 gaaaatggct accagactaa aatttcaagc tttgaagatt atctcagtcg agccaacgaa
     9061 cttggtcaaa aattactaat tgaaattaaa accagtaaaa aagatagtcc agacatgatg
     9121 aaccgctttt tagcccgtta tgctgcaaag ctcaagattt atggacatca aatccagtct
     9181 ttagactacc atgttgtcga aaaagtaaga cagtatgatg ccgaactacc agtttatttc
     9241 atcatgccct acaattctgt ctttcctaaa acaagagcga caggatatac tatggagtac
     9301 tcaaccttgg atgaatattt tgtaagcaaa ctatggacaa cggatcagaa actttatgtc
     9361 tggactgtca atgattcaga agctatcagc aaatctcttc acttaggagt agatggagtg
     9421 attacagatg atttggaaaa agttcagcgg gaaatagaag tagcccaaga agatccagaa
     9481 tatacggatt tgctcttgaa aaaagcattg gaattctttg aattttagta ataaaaaaga
     9541 ggaacggttg ttcctcccat gataggtaag tattcttgtg aatgcttgct tttttgtggt
     9601 tgcaatccgt ttacgagaat tgtttgtatt gttcttgttt caatttactt tatagtgctt
     9661 tgaagttgga atactacact ataacttcta aaatatagta aaatgaacca aaaatagtac
     9721 acaatgtggt gtaatctttt tatggcatat tcaatagatt ttcgtaaaaa agttctctct
     9781 tattgtgagc gaacaggtag tataacagaa gcatcacacg ttttccaaat ctcacgtaat
     9841 accatttatg gctggttaaa gctaaaagag aaaacaggag agctaaacca ccaagtaaaa
     9901 g
//
LOCUS       DAVDWM010000062         9842 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVDWM010000062 DAVDWM010000000
VERSION     DAVDWM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.062"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            343..6777
                     /locus_tag="VUW49_001928"
     CDS             343..6777
                     /locus_tag="VUW49_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7981798.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARVKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDDRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYINGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNLNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
     gene            7404..7880
                     /locus_tag="VUW49_001929"
     CDS             7404..7880
                     /locus_tag="VUW49_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7981799.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7918..8223
                     /locus_tag="VUW49_001930"
     CDS             7918..8223
                     /locus_tag="VUW49_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7981800.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            8271..9746
                     /locus_tag="VUW49_001931"
     CDS             8271..9746
                     /locus_tag="VUW49_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET7981801.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT         3618 a         1480 c         1903 g         2841 t
ORIGIN      
        1 acaacctcaa aacagtgttt tgagctgact acgtcagttc catctacaac ctcaaaacag
       61 tgttttgagc aacctgcggc tagtttccta gtttgctctt tgattttcat tgagtactaa
      121 tcttactttt taacgtgtgc taaaaaataa attaatgcat gttaattttt cttgaattaa
      181 atttttttaa atgatatact gtttttatag agagataaca attatatata aatgtactac
      241 tctatttcct agataatact tagtaaaact ttttataaca aaggctagca aagttaaagt
      301 ttattatcta ttggaagtta aataaatatg taaggaatta aaatgaaaaa aagtacagta
      361 ttgtcattaa ccacagctgc agttatttta gcagcctatg cccctaatga ggtagtctta
      421 gcagacacat ctagctctga agatgcttta aacatctctg ataaagaaaa agtagtatta
      481 gataaggaaa cagaaaataa agagaaacat gaagatattc ataatgctat agaaacttca
      541 caggatttta aagagaagaa aacagcagtc attgagggaa aagaagttgt tagtaaaaat
      601 cctgtgacag acaataacac tagcaatgaa gaagcaagag tcaaagaaga ttccaatcaa
      661 tcccaaggag atcatacgga ctcatttgtg aataaaaaca cagaaaatcc caaaaaagaa
      721 gataaagttg tctatattgc tgaatttaaa gataaagaat ctggagaaaa agcaatcaag
      781 caactatcca atcttaagaa tacaaaagtt ttatatactt atgatagaat ttttaacggt
      841 agtgccatag aaacaactcc agataacttg gacaaaatta aacaaataga aggtatttca
      901 tcggttgaaa gggcacaaaa agtccaaccc atgatgaatc atgccagaaa ggaaattgga
      961 gttgaggaag ctattgatta cctaaagtct atcaatgctc cgtttgggaa aaattttgat
     1021 ggtagaggta tggtcatttc aaatatcgat actggaacag attatagaca taaggctatg
     1081 agaatcgatg atgatgccaa agcctcaatg agatttaaaa aagaagactt aaaaggaact
     1141 gataaaaatt attggttgag tgataaaatc cctcatgcgt tcaattatta taatggtggc
     1201 aaaatcactg tagaaaaata tgatgatgga agggattatt ttgacccaca tgggatgcat
     1261 attgcaggga ttcttgctgg aaatgatact gaacaagata tcaaaaactt taacggcata
     1321 gatggaattg cacctaatgc acaaattttc tcttacaaaa tgtattctga cgcaggatct
     1381 gggtttgcgg gtgatgaaac aatgtttcat gctattgaag attctatcaa acacaacgtt
     1441 gatgttgttt cggtatcatc tggttttaca ggaacaggtc ttgtaggtga gaaatattgg
     1501 caagctattc gggcattaag aaaagcaggc attccaatgg ttgtcgctac gggtaactat
     1561 gcgacttctg cttcaagttc ttcatgggat ttagtagcaa ataatcatct gaaaatgacc
     1621 gacactggaa atgtaacacg aactgcagca catgaagatg cgatagcggt cgcttctgct
     1681 aaaaatcaaa cagttgagtt tgataaagtt aacataggtg gagaaagttt taaatacaga
     1741 aatatagggg cctttttcga taagaataaa atcacaacaa atgaagatgg aacaaaagct
     1801 cctagtaaat taaaatttgt atatataggc aaggggcaag accaagattt gataggtttg
     1861 gatcttaggg gcaaaattgc agtaatggat agaatttata caaaggattt aaaaaatgct
     1921 tttaaaaaag ctatggataa gggtgcacgc gccattatgg ttgtaaatac tgtaaattac
     1981 tacaatagag ataattggac agagcttcca gctatgggat atgaagcgga tgaaggtact
     2041 aaaagtcaag tgttttcaat ttcaggagat gatggtgtaa agctatggaa catgattaat
     2101 cctgataaaa aaactgaagt caaaagaaat aataaagaag attttaaaga taaattggag
     2161 caatactatc caattgatat ggaaagtttt aattccaaca aaccgaatgt aggtgacgaa
     2221 aaagagattg actttaagtt tgcacctgac acagacaaag aactctataa agaagatatc
     2281 atcgttccag caggatctac atcttggggg ccaagaatag atttactttt aaaacccgat
     2341 gtttcagcac ctggtaaaaa tattaaatcc acgcttaatg ttattaatgg caaatcaact
     2401 tatggctata tgtcaggaac tagtatggcg actccaatcg tggcagcttc tactgttttg
     2461 attagaccga aattaaagga aatgcttgaa agacctgtat tgaaaaatct taagggagat
     2521 gacaaaatag atcttacaag tcttacaaaa attgctctac aaaatactgc gcgacctatg
     2581 atggatgcaa cttcttggaa agaaaaaagt caatactttg catcacctag acaacaggga
     2641 gcaggcctaa ttaatgtggc caatgctttg agaaatgaag ttgtagcaac tttcaaaaac
     2701 acggattcta aaggtttggt aaactcatat ggttccattt ctcttaaaga aataaaaggt
     2761 gataaaaaat actttacaat caagcttcac aatacatcaa acagaccttt gacttttaaa
     2821 gtttcagcat cagcgataac tacagattct ttaactgaca gattaaaact tgatgaaaca
     2881 tataaagatg aaaaatctcc agatggtaag caaattgttc cagaaattca cccagaaaaa
     2941 gtcaaaggag caaatatcac atttgagcat gatactttca ctataggcgc aaattctagc
     3001 tttgatttga atgcggttat aaatgttgga gaggccaaaa acaaaaataa atttgtagaa
     3061 tcatttattc attttgagtc agtggaagaa atggaagctc taaactccaa cgggaagaaa
     3121 ataaacttcc aaccttcttt gtcgatgcct ctaatgggat ttgctgggaa ttggaaccac
     3181 gaaccaatcc ttgataaatg ggcttgggaa gaagggtcaa gatcaaaaac actgggaggt
     3241 tatgatgatg atggtaaacc gaaaattcca ggaaccttaa ataagggaat tggtggagaa
     3301 catggtatag ataaatttaa tccagcagga gttatacaaa atagaaaaga taaaaataca
     3361 acatccctgg atcaaaatcc agaattattt gctttcaata accaagggat caacgctcca
     3421 tcatcaagtg gttctaagat tgctaacatt tatcctttag attcaaatgg aaatcctcaa
     3481 gatgctcaac ttgaaagagg attaacacct tctccacttg tattaagaag tgcagaagaa
     3541 ggattgattt caatagtaaa tacaaataaa gagggagaaa atcaaagaga cttaaaagtc
     3601 atttcgagag aacactttat tagaggaatt ttaaattcta aaagcaatga tgcaaaggga
     3661 atcaaatcat ctaaactaaa agtttggggt gacttgaagt gggatggact catctataat
     3721 cctagaggta gagaagaaaa tgcaccagaa agtaaggata atcaagatcc tgctactaag
     3781 ataagaggtc aatttgaacc gattgcggaa ggtcaatatt tctataaatt taaatataga
     3841 ttaactaaag attacccatg gcaggtttcc tatattcctg taaaaattga taacaccgcc
     3901 cctaagattg tttcggttga tttttcaaat cctgaaaaaa ttaagttgat tacaaaggat
     3961 acttatcata aggtaaaaga tcagtataag aatgaaacgc tatttgcgag agaccaaaaa
     4021 gaacatcctg aaaaatttga cgagattgcg aacgaagttt ggtatgctgg cgccgctctt
     4081 gttaatgaag atggagaggt tgaaaaaaat cttgaagtaa cttacgcagg tgagggtcaa
     4141 ggaagaaata gaaaacttga caaagacgga aataccattt atgaaattaa aggtgcggga
     4201 gatttaaggg gaaaaatcat tgaagtcatt gcattagatg gttctagcaa tttcacaaag
     4261 attcatagaa ttaaatttgc taatcaggct gatgaaaagg ggatgatttc ctattatcta
     4321 gtagatcctg atcaagattc atctaaatat caaaagcttg gcgagattgc agaatctaaa
     4381 tttaaaaatt taggaaatgg aaaagagggt agtctaaaaa aagatacaac tggggtagaa
     4441 catcatcatc aagaaaatga agagtctatt aaagaaaaat ctagttttac tattgataga
     4501 aatatttcaa caattagaga ctttgaaaat aaagacttaa agaaactcat taaaaagaaa
     4561 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4621 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4681 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4741 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4801 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4861 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4921 gatatgagat tatttgttaa agatgatgat cgaataaaag ctgaaactaa gattagaatg
     4981 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     5041 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     5101 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5161 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5221 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5281 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5341 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5401 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5461 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5521 tccaatgtag aagctataaa agaagatggt tccatgttat ttattgatac caaaccagta
     5581 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5641 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5701 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5761 actagagatt ttaatattaa gttgaatgtt aaagacggtg acatcatgga ctggggaatg
     5821 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5881 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5941 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     6001 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     6061 gagattcaag aacaacatat ttatataaat ggaaaagaat atacatcgtt tgatgatatt
     6121 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6181 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtcagtga attaaaacct
     6241 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6301 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6361 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6421 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6481 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6541 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6601 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6661 aatcttaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6721 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagat
     6781 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6841 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6901 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6961 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     7021 gatgagacaa tttcaaggaa ttaagtagcg tttggttgct taattccttt ttatttggaa
     7081 ggaagttttt cgtaaccgcc caataacgaa gtctattgga cggttacgat atgatcactg
     7141 acttcgtcag tcttatctac aacctcaaaa ctgtgttttg agcaacctgc ggctagcttc
     7201 ctagcttact ctttgatttt cattgagtat tagaacagaa aaaatgcttg gagtatttgt
     7261 ttgtgtgttt atttttatat aacaaactat aaacaaaaca aaaatataaa aaaagagaca
     7321 aaaaagaaca gaaagtaatt gacaacggtt tcatatagtg ttatacttat ggcataaagt
     7381 taatggaaag aagggaaaaa cttatggtat tagattattt ctttgacaaa aaccttgtgt
     7441 tttgcttaga agcggataat caagaacaac tctttgatca ggttgcaact ttattggaag
     7501 aacgagaaat tgtcactcca acttatcgtg aagctttgat cacgcgtgaa aagtcatttc
     7561 caactggttt agatatggaa tttctaggaa aggacttgcc aaatgtagcg attcctcata
     7621 cagatattgt tcataatcta gctgagaaag tggtggttgt tcgattagaa aaaccagtaa
     7681 cttttcacaa tatgatagct cctgataagg aagtagaagt atccttgctc ttctttatca
     7741 tcaataactc aagttcaagt caaacaaata ttctggctca gttgatggac tttttcacag
     7801 gaaatggaca tcttgaagac ctatcaaaaa tttccgaacc agaaaaactt tatgcttaca
     7861 ttgctgaagc aaccgcttaa tcttgtctat taaaaaataa aatcggagga aatccaaatg
     7921 attaaaattc ttgctgcctg cggtgcaggt gttaactcaa gtcaccaaat taaaagtgct
     7981 ctagaagaag aactttcaaa ccgtggttat gatgttcact gtgatgcagt catggtgaaa
     8041 gatgtaaacg aagaccttat gaaaggttac gatatcttta caccaatcgc tgcaacagac
     8101 cttggttttg aaccaggtat cccagttatc gaagctgggc caatcttatt ccgtatccca
     8161 gcaatgagcg ctccagtatt tgacaatatt gaagcagcta ttaaagaaca cggattaagc
     8221 taattctaat ttgttaacat tcatataaca taaaaaaggg aggaactgtt atggatgcaa
     8281 tctttgacct aatcggaaag gttttcaatc ccattttaga aatgggtgga cctgtcatca
     8341 tgttaatcat tttgacagta ttggctttac tttttggagt gaaattctcc aaagcgcttg
     8401 aaggtggtat caaacttgcc atcgctctta caggtatcgg tgctatcatc ggtatgctaa
     8461 atggtgcttt ctcagcatca cttgcaaaat tcgttgaaaa cactggtatc caattgagta
     8521 ttaccgacgt tggttgggca ccactcgcaa caatcacttg gggttctgct tggacactat
     8581 acttcttgct catcatgttg attgtcaaca tagtgatgct agctatgaag aaaaccgata
     8641 cacttgatgt cgatatcttt gatatctggc acttgtctat cacaggtctc ttgattaaat
     8701 ggtatgctga taacaatggt gtgagtcaag gggtttcact ctttattgct acagcagcta
     8761 tcgttcttgt cggtgtgttg aaaattatca actctgactt gatgaaacct acatttgatg
     8821 accttcttaa cgccccaagt tcatcaccaa tgacatcaac tcacatgaac tacatgatga
     8881 acccagttat catggttttg gataagattt ttgaaaaatt cttcccaggc cttgataaat
     8941 atgactttga tgctgctaaa ttgaacaaga aaatcggttt ctggggatct aaattcttca
     9001 tcggtttcat ccttggtatc gttatcggta ttatgggaac tccacatcca attgcaggtg
     9061 ttgcagatgc agataaatgg cgtcttgtta tcaaaggatg gttgtctctt ggtttgactg
     9121 ccggtgtatc tttggaactc ttctcactta tcggttcatg gttcatcgca gccgtagaac
     9181 cactatcaca aggtattaca aacgttgcta ctaaacgtct tcaaggacgt aaattcaata
     9241 tcggtcttga ctggccattc atcgctggtc gtgctgaaat ctgggcttgt gccaacgtac
     9301 ttgcaccaat tatgttgatt gaagcagtgc ttctttcaaa agttggaaat ggtatcttgc
     9361 cacttgcagg tatcatcgct atgggtgtta ctccagctct cttggttgta actcgtggta
     9421 aattgctccg tatgattatc ttcggaacac tcttgttgcc actcttcctt ctttcaggta
     9481 cacttattgc accatttgca acagaacttg ctaaaggtgt aggtgccttc ccagaaggtg
     9541 tgagccaaac tcaattgatt actcactcta ctcttgaagg accaatcgaa aaacttcttg
     9601 gttggacaat tggtaacact acaactggtg atatcaaagc aatccttggt gcagtagcct
     9661 tccttgtatt ctatatcggt atctttgctt ggtacagaaa acaaatgatc aaacgtaacg
     9721 aagagtacgc agcaaaagca aaataatgcg ctcctataaa atataaactc aatgaaaatc
     9781 aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac acagttttga ggttgtagat
     9841 aa
//
LOCUS       DAVDWM010000063         9796 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVDWM010000063 DAVDWM010000000
VERSION     DAVDWM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.063"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1..601
                     /locus_tag="VUW49_001932"
                     /pseudo
     CDS             1..601
                     /locus_tag="VUW49_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            529..978
                     /locus_tag="VUW49_001933"
     CDS             529..978
                     /locus_tag="VUW49_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981802.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALS"
     gene            1813..2169
                     /locus_tag="VUW49_001934"
     CDS             1813..2169
                     /locus_tag="VUW49_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981803.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            2174..2539
                     /locus_tag="VUW49_001935"
     CDS             2174..2539
                     /locus_tag="VUW49_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HET7981804.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            2526..4349
                     /locus_tag="VUW49_001936"
     CDS             2526..4349
                     /locus_tag="VUW49_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HET7981805.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            4463..5329
                     /locus_tag="VUW49_001937"
     CDS             4463..5329
                     /locus_tag="VUW49_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981806.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
     gene            5920..6171
                     /locus_tag="VUW49_001938"
     CDS             5920..6171
                     /locus_tag="VUW49_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981807.1"
                     /translation="MSSQMKAFLNELQGNMEVVGEFFDNPEKVIRKFGIVGREKEALL
                     IRDLNDMDDYALSIQNSVASPSGAHSSTCHFVRQDLQSA"
     gene            6400..6702
                     /locus_tag="VUW49_001939"
     CDS             6400..6702
                     /locus_tag="VUW49_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981808.1"
                     /translation="MKNKYDVKRIIPDELSESLDIFLKNYSETGLSDYNTYLFYGFIL
                     KSYKLPRENRYSIKLLVKELQNRGLKVTLIINIYYHALNCLALNDGLKIYGEDFLI"
     gene            6699..8045
                     /locus_tag="VUW49_001940"
     CDS             6699..8045
                     /locus_tag="VUW49_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7981809.1"
                     /translation="MNYEKYLNYLDYETEIEDAYHNLLLEYKISDNFSDEHWLYNLPS
                     NITQSKGFKIHLSASILNANLVAKKFFDFIFSREKKINFKILVSIKELSLQNTGLNGY
                     SQVGKFITIYPKDNKEFQRLLHKLEILYKGVKGVNIPSDFRFQLSEVVYYRYGEFVKD
                     STFKDKRDKKIPSNVNVPIRDYYIPRYNTIPDQYIILEVISKNAKGGVYKVFNTQKRV
                     YSLLKEASDLSLVDFTNRDSVNRLINEREILVELEKEEFTPKVFNDFYIKNSYFVEFE
                     FIIADKLSEYNMVSGSYDWFLTLIDYMEIINSKYNLSYRDLSFNNILIDNRNNIYIID
                     FEHALRKETQIEEKKLPLFGTPGFYETNLNLLNNQPEDIMGLVLLLYWSQNLDDFNVF
                     SKMSFLEAMEYAMNFNSRRLDTLDKSDWLYDIYKKAFNYKYDTFSELKKDFIEVLK"
     gene            8059..8976
                     /locus_tag="VUW49_001941"
     CDS             8059..8976
                     /locus_tag="VUW49_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981810.1"
                     /translation="MNCFLKMNNVSVRYDDVIALKDITLQINKGDFIGLLGSNGAGKS
                     TLINSIVGFQEIYLGEIEYCDKDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLN
                     LAGKFGKNAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVQIARAIAHNPDFYILDE
                     PTVGLDTESAEKFLMYLKDKSLEGKTIIISSHDINLLEKFCKKILFLQNGSISFFGDM
                     RDFVDNSTIKLNFSMQNRISRYQIEFLENFRFKVHIEDNDSFTIEVPIEENILDVINE
                     VGKACEIKNFSTSKLTLQESYLQRIGGEK"
     gene            8973..9773
                     /locus_tag="VUW49_001942"
     CDS             8973..9773
                     /locus_tag="VUW49_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981811.1"
                     /translation="MKADQLRHKSDLGLRGLAIIAKNEIIAFFRSKGLIISQFLQPIL
                     YVVFIIIGLNSSIKNIQFNDIKTSYAEYTIIGVIALLIIGQMTQVIYRVTIDKKYGLL
                     ALKLCSGVRPLYYILGMSIYSILGLIVQEIIIYIITLAFEINIAMDRFFYTVLLSIVV
                     LLFWDSLAILLTMFINDYRRRDIVIRFVLTPLGFTAPVFYLIDSAPSIVRWIGQLNPL
                     TYQLTILRNFYFKNSTTLELVFLLLTSLLVLISVSFIIPKIKLILIER"
BASE COUNT         3528 a         1274 c         1839 g         3155 t
ORIGIN      
        1 tatgatatat tttttgaaaa taagaaaata aaagttgata agacaatttt ttttagtaat
       61 tttgataagg ttgttttttc tagttcaaaa gaatatatta gttttcttat gttagcagag
      121 gcaatcataa aaaatggaat agaaatagtt gtagtaactg atcataatac taccaaaggt
      181 attaaaaagt tacaaatggc agtctcaatc ataatgaaaa attatccgat ttatgatata
      241 catcctcata ttttacatgg agtagaaatt agtgcagcag ataaattgca tattgtatgt
      301 atatatgatt atgaacaaga atcatgggtt aatcaatggt taagtgaaaa tattataagt
      361 gagaaagatg gaagttatca acattcactg actataatga aggatttcaa taatcaaaaa
      421 atagttaact atattgctca tttcaatagt tatgacattt tgaaaaaagg ttctcactta
      481 tcaggtgcat ataaacgaaa aattttttct aaagaaaata cacaattttt ggagtttaat
      541 attaactcga aagaatcttc gcaacaactt gatattctct ataaagaagt tggtgtatta
      601 agtttgggac aaaaagttgt agccatgctt gattttttat tagcatatag tgattattct
      661 aaagacttca gaccattgat tattgatcag cctgaagaca atctagacaa tcgttatatt
      721 tacaggcatt tagttcagca gtttagagat gtgaaagctc aacgtcaaat tattttagca
      781 acacataatg ctacaattgt aacaaattct atgacagatc aagttgttat tatggagtca
      841 gatggagtta acggatggat tgaatcacag ggatatgtta gtgaaaaata tataaaaaat
      901 catatcatca atcaattaga gggaggaaaa gattccttca agcataaaat gtctatatat
      961 gagacggctt tatcatagta gagtcagaaa aagtaggcta gaaatttagc ctactttttt
     1021 ctttgttcga caggcatagt gtacatctga ggtccaagtc ctctgtggat atttgctgca
     1081 gatgaaacca atagcgactc ctaatcctga atatcgtgag gtagggggga taggaaggaa
     1141 ttagcgaaat caaggttcta caaacagaat cgtgacttga agccatatat agcggatgag
     1201 gaactctaaa atccaaatag gtgtcgtaac ctatatacgt aaattacgag agtaaactag
     1261 gaaggatgta cggcttattc cgtgagcatt taggacgtag tacaacgaat catgggagtc
     1321 agctgaacac atagtattga agaaatttct gtaatggaaa tggagcgaag aagtgaacaa
     1381 ttaaatgaat acctctctaa ttaaatttgt caattctaat tcctggtatg aaaagacagt
     1441 gacctgaaaa tgtaaacgat gggagctgat cataaatata ggacggtaca tgcagtggtg
     1501 ttagagatta gtccctactt gatttgtgat aacttcccca aatttcttct gctatacttt
     1561 tctcaacttt taaaaatcca actaagaatt ttacctggga gtttgggggc gtagccacca
     1621 agttatctta tggttagctg tcaaaactgg aaggttttgg ttagctggcg atatgatttt
     1681 ttgggtattg tgaccacaat gcctgagctc gcaaagcctt acaagaatga aaatcagttg
     1741 ttggaaaagt gtactgacat tgtatggtag ctcacattgt cagtacaagt attttggaaa
     1801 ggaagtagca gtatgaaacg agatgtgcgt gatattcgga aacaatttcg tttaacagaa
     1861 gcagaagaaa agcaaattct agctttgatg agagagcggg gagagactaa tttctctgat
     1921 tttcttcgta aaagtttact ttcctctgat ttacaaaaac agatggagac atggtttgcc
     1981 ctctggcaat cccaaaaact agaacaaatc agtcgtgacg ttcatgaagt tttaatcttg
     2041 gcacagtcag aacgtcaagt cacccaagag catgtatcta ttctcttaac gtgcgtgcag
     2101 gaattgattc aagaggttgc aaacaccata cccctcagta aagaatttcg tgagaagtac
     2161 atgaggtaag cacatggaac atcgttaccg aaccaatctc aagaaagtgt ttttgtctga
     2221 tagtgagttg aaccaactaa atataaatat cgatcaaagt ggttgtaaat ccttttctga
     2281 atatgcgaga cgaactctac tcgatcctgg tatgaatttt atcacgattg acacaaacgg
     2341 ttaccaagat ttagtgtttg agttaaagag gattggcaat aatatcaacc agattgctcg
     2401 aagtgttaat caatctcagt taatttctgg tgaagaattg caggagttga aaaaaggaat
     2461 tggtgaattg ataaaagaag ttgataagga atttaatctg caagcgcaga agctaaagga
     2521 gttccatggt catcactaaa cactttgcca ttcacggaaa gagttaccgc agaaagctta
     2581 tcaagtacat tctcaatcct gagaaaacca ataatcttgc cttggtgtcg gactatggca
     2641 tgaagaattt tctggacttt cctagctatg aggaaatggt gcagatgtat catgaaaatt
     2701 tcatcagcaa cgatacgctt tacgattttc gccacgacag gatggaagaa aatcaacgaa
     2761 aaatacacgc tcaccacatc attcagtctt tctcgccaga ggatcatctc actcctgaac
     2821 aaatcaatcg gataggttat gagactgtga aggaattaac tggtggcaaa tttcgtttta
     2881 tcgttgcgac ccatgttgat aaagaccacc tgcacaatca catcattatc aattcagtag
     2941 atagcaattc tgacaaaaag ctcaagtggg actacaaggt ggagcgaaat cttcgcatga
     3001 tttctgaccg tttttctaaa atcgcaggtg ctaaaatcat tgagaaccgc tattctcacc
     3061 agcggtatga agtctatcgt aagactaatc acaagtatga actcaagcag cgactctatt
     3121 ttttgatgga acattctagg gactttgagg atttcaaaaa gaatgctccg ctactacatg
     3181 tggagatgga tttccgtcac aagcatgcca ccttttttat tacggactca actatgaaac
     3241 aggtggtgcg tggcaagcaa ctcaatcgca agcagcctta cacagaagaa ttttttaaga
     3301 actactttgc caaaagagaa atagaaagtc tcatggaatt tttattgctg aaagttgaga
     3361 atatggatga tttacttcag aaagcaaaac tttttggact aactatcaat cctaaacaaa
     3421 agcatgtttc ttttcaattt gcaggagtgg aggtaaagga gacagagcta gaccagaaaa
     3481 atctttatga tgtagagttt ttccaagatt attttaaaaa tagaaaagat tggcaagctc
     3541 cagaaactga ggatttcgtt caactttatc aagaagaaaa gttatccaaa gaaaaagaac
     3601 ttccaagcga tgagaagttc tgggagtcct atcaagagtt caagagtaac agagatgccg
     3661 ttcatgaatt tgaggtggag ttgtcactca atcaaattga aaaagtagtg gatgatggaa
     3721 tttacgtcaa ggtcaagttt ggtattcgtc aggagggact tatctttgtg ccgaacatgc
     3781 agcttgatat ggaagaggat aaggtgaagg ttttcatcag ggaaaccagc tcctactatg
     3841 tctaccacaa agacgctgcc gagaaaaatt gttatatgaa aggtcgaacc ttaattagac
     3901 agttcagcta tgaaaatcaa accattccat tacgcagaaa agcgacagtc gatatgatta
     3961 aagagaagat tgcggaagtg gatgctttga ttgaactgga agtagaaaat caatcttatg
     4021 tcacgattaa agatgagtta gtgcatgaac tagcagcgtc tgaattgaga atcaatgagt
     4081 tgcaagaacg aatgtcaacc ttgaatcaag tagcagaata tctactggct tcagttgaaa
     4141 gtaagcaaga aatgaaatta aatctttcaa aactgaatat aactgagaat atcagtgcta
     4201 atattgttga gaaaaaattg aagagcctgg ggaatcaact ggaattggaa aggggcaggt
     4261 atgaaaagat ggtagtcagg ctagataaat ttataaatcg cttaaataca ggactttcaa
     4321 aaggagatgg gatagatttt caaaaatagg aatctagcca ataattatat acaatgtgta
     4381 tcttttgtaa aatatatttg aaatttagat ttaatcatgt tacaattaac attgaaatca
     4441 aatagaaaag gaagatgcaa acgtgggtac actattagca acaagattaa aaaatagacg
     4501 aaaagaatta aaaatgtctc agcgagaatt agctgagggt atctgtaaac aggggcaaat
     4561 tagtcgatta gagagtggag agttcacccc tggagcggat tttttacatg cccttgctaa
     4621 gaaattaaaa gttagtatgg actacttttt tgatgagcag gttgtagagg aagtcaatga
     4681 gttatcagag tttaagaagt tagcacagac atttatcaca aatcgtaatt atgaatcttt
     4741 aaaatatata tatgaattgg aaaatgtaaa agcccatcgc ttatctctag cagataaatt
     4801 ttacatggaa tggataaagt ctcttataga tttttatttt tatggacaga aagaagaggc
     4861 aattacacga ttagagaata tgctacctca gttaaatgta tctgatttga actaccttca
     4921 agtttcaaac actctattca atttttatta tgatattgaa gatttagaac gttttaatga
     4981 gattagagac aagctagagc atcaagtgaa tcagcttaat ttaaacacaa ttgaagagtt
     5041 agaactatca attaagttca attataacgt ttgtcgatac ctatggttac agaaaaatat
     5101 tgaagaagct attacgaaaa tcacagctac gataaagcaa tgtaaggagt acaggacaac
     5161 ttatctatta gctgatttgt atttattgat gggaagtgta agtgaaaatt tttcttctaa
     5221 aagttcagta aaagaatatt ttgagactgc tcattttctc tacaaacttg aggaaaacat
     5281 gtcaatggct ttgaaagtcg aacattattt tgcagatata acagaataag gaatacggtg
     5341 ttctagtgag catcgttttt taaatctaat ttgaatacaa aaaagtatta aataaaaaaa
     5401 aataaaaaaa gattataaaa atacttgaca agctaatttt gaagtgataa aataaatgca
     5461 agaaaacgaa aggagaggta tatgagaagt ttgtttagga aaattgttgc tttgttagtc
     5521 ataggattaa tcctattagg gactgctgga ggaactcaag ttcataagat ggctagaggg
     5581 attgatccag gtcctgcgaa tggtatttat agataggaga ttatagtcac ttaaaaatca
     5641 aaaaattata aaatttttgt tttacttaaa tagatatttt atgatgggcg aagtttttat
     5701 aactagttcc tataagtaca gaaacctatt ttaatttact aatttcaaaa caattttttg
     5761 agatatatgt aattaaatct aaattaagct ttaaaaaatt atttttagca atttgatttt
     5821 gaaagtgtca tgcagcaata tgaaagcgta gaatagaggt gtttccaatg tacaagtaag
     5881 ttttgattcc aaataaagta atagaaaagg agctatataa tgagtagtca aatgaaagct
     5941 ttcttgaatg aacttcaagg aaatatggaa gttgttggag aattttttga taatcctgaa
     6001 aaggtaatta gaaaatttgg aattgtcgga agagagaagg aagcattgct aatccgtgac
     6061 ttaaatgata tggatgatta tgctctttca attcaaaata gcgttgcatc gccttctggg
     6121 gcacatagta gcacttgcca ttttgttaga caagatttac aatccgcata attgttaata
     6181 tataaaacat aaaatttcaa ttttcaatta gggataatag aattccttac tatgatatag
     6241 aaagtgaatt aattaggaaa tcttagtcag aggtttctga ttaattcatt ttttatatat
     6301 taagagtgat gtgaaatgtt caaattgtga gactactctc ggattgttgt atagaaattt
     6361 aaaattttat tgtgagcttt ttaaggaaga gtggccgata tgaaaaataa atacgatgtt
     6421 aaaagaatta ttccggatga attatcagaa tcactagata tttttctaaa aaattattct
     6481 gaaactggat tgtcagatta taatacttat ctcttttatg gttttatctt gaagtcctat
     6541 aaattgcctc gagaaaatag atatagcata aaattattgg tgaaggaatt acaaaataga
     6601 ggactgaaag ttacattgat tatcaacatt tattatcacg ctttaaattg tttggcttta
     6661 aatgatggtc taaaaattta tggagaagat ttcttaatat gaattacgaa aagtatttaa
     6721 actatttgga ctatgaaact gaaatagaag acgcatacca taatttatta ttagaatata
     6781 aaattagtga taattttagt gatgaacatt ggttatataa tttgccgtct aatatcaccc
     6841 aatctaaggg atttaaaata cacctgagcg cttcaatatt aaatgctaat ttggttgcta
     6901 aaaaattctt tgattttata ttttctagag agaagaagat aaatttcaaa attttggtta
     6961 gcattaaaga actttctttg caaaatacag ggcttaatgg ttattcacaa gtcgggaaat
     7021 tcattacaat ttatccaaaa gataataaag agtttcaacg tttgttacac aaattagaaa
     7081 tattgtataa aggtgtaaag ggtgtaaaca taccatcaga tttcagattt caacttagcg
     7141 aggttgtata ttacagatat ggggagttcg taaaagatag cactttcaag gataaaagag
     7201 ataagaaaat cccttcaaat gtgaatgttc caataaggga ttactatatt ccaagatata
     7261 acactattcc tgatcaatat attattttgg aagtgatttc taaaaatgcc aaaggtggag
     7321 tttataaagt atttaatact caaaagcgag tttattcttt attaaaagaa gctagtgatt
     7381 tatcgttggt tgattttaca aatagagatt ctgtgaatcg tttaataaat gaaagagaaa
     7441 tattagtaga attggaaaag gaggaattta ctccaaaagt ttttaacgat ttttacataa
     7501 agaattcgta ttttgtggaa tttgagttta ttattgcaga taaattaagc gagtataata
     7561 tggttagtgg ctcgtatgat tggtttttaa cattaattga ttatatggag ataataaact
     7621 ccaaatataa tctttcctat agagatttat catttaataa tatacttatt gataatagaa
     7681 ataatattta tattatagac tttgagcatg ccctaaggaa agaaactcaa attgaagaaa
     7741 aaaagttgcc tctatttgga actccagggt tttatgaaac caacttaaat ttattgaata
     7801 atcaaccaga ggatattatg ggattggttt tgttattata ttggagtcag aatttagatg
     7861 attttaacgt cttttcaaaa atgagttttt tagaagcgat ggagtatgca atgaatttta
     7921 attcgcgtcg cttagacacg cttgataaat cagattggct ttatgatatt tataaaaaag
     7981 cgttcaatta taaatatgat actttttctg aattaaaaaa agattttata gaggtattaa
     8041 aataaaggta ggggtgttat gaattgtttc ttgaaaatga ataatgtaag tgttcgttat
     8101 gatgacgtaa tagctttaaa agatataact ttacaaataa ataagggaga tttcattggc
     8161 ttattaggtt caaatggtgc aggtaaatct acgttaatta attctattgt aggttttcaa
     8221 gagatttatt taggagaaat agagtattgt gataaagatt tgatagttag ttctcaacct
     8281 tttgctcatt taggctttac tcctcaaacc acagtaattg atttttatac tactgtgaag
     8341 gacaatgtaa tattggggct gaaccttgct ggaaagtttg ggaaaaatgc tgagaagttg
     8401 tgtcaaatag ccttagaaat tgttgggtta gctgataaaa aaaataattt ggtagaaaca
     8461 ttgtcaggtg gacaactgca acgcgtccag attgctagag caatagctca taatccagat
     8521 ttttatattt tagatgaacc taccgttggt ttagatactg aatctgccga aaaattttta
     8581 atgtatttaa aagataagag tttggaagga aaaactatta tcatatcttc acatgacata
     8641 aatctactcg aaaagttttg taaaaaaata ctttttttac aaaatggctc catatcattt
     8701 tttggtgata tgcgtgactt tgtagataat tcaactatca aattaaattt ttcaatgcag
     8761 aatagaattt ctagatatca aattgaattt ttagaaaatt ttagatttaa agttcacatc
     8821 gaagataatg atagttttac aatagaagtc cctatagaag aaaatatctt agatgttatc
     8881 aatgaggtag gaaaagcatg tgaaattaaa aacttttcaa caagtaaatt aaccttacaa
     8941 gaaagttatt tgcaaagaat aggaggagaa aaatgaaggc tgatcaatta aggcacaaat
     9001 cggacttagg tttaagaggt ctagcgatta ttgctaaaaa tgagattatt gcttttttta
     9061 gaagtaaagg tttaattatt tctcagtttc tacaaccaat cttatatgtt gtttttataa
     9121 taataggatt aaattcttcg ataaagaaca ttcagtttaa tgatataaaa acctcttatg
     9181 cagaatatac aatcattggt gttatagctt tattgataat cgggcagatg actcaagtta
     9241 tttatagggt gacaatagat aaaaaatatg ggctacttgc tcttaagtta tgcagtggag
     9301 ttcgtccttt atattatatt ttagggatga gtatctattc tatattaggg ttgatagttc
     9361 aagaaattat tatatatata attacgttag cgtttgagat aaatatcgca atggatagat
     9421 ttttttatac agttttgtta tctattgttg ttttattatt ttgggactcc cttgcaattt
     9481 tacttacaat gtttatcaat gattacagaa gacgtgatat tgtaatacgt tttgtactaa
     9541 caccgcttgg ttttacagct cctgttttct acttaataga ttctgctcct agtattgtga
     9601 gatggattgg tcagttaaat cccttaactt atcaattaac tattttgaga aacttttatt
     9661 ttaaaaattc aacaactttg gaattagttt tcttattgtt aacatcatta cttgtcctta
     9721 tatctgtatc ttttattata ccaaagataa aattgatact gatagaaaga taaaagttgg
     9781 gtcatccaac tttttt
//
LOCUS       DAVDWM010000064         9189 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVDWM010000064 DAVDWM010000000
VERSION     DAVDWM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.064"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(724..984)
                     /locus_tag="VUW49_001943"
     CDS             complement(724..984)
                     /locus_tag="VUW49_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981812.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(1043..1594)
                     /locus_tag="VUW49_001944"
     CDS             complement(1043..1594)
                     /locus_tag="VUW49_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981813.1"
                     /translation="MPANTKVIFQETFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1584..2579)
                     /locus_tag="VUW49_001945"
     CDS             complement(1584..2579)
                     /locus_tag="VUW49_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981814.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLTKGQKVVNVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPFSQHILKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(2987..3412)
                     /locus_tag="VUW49_001946"
     CDS             complement(2987..3412)
                     /locus_tag="VUW49_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981815.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVTSISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
     gene            complement(3541..3924)
                     /locus_tag="VUW49_001947"
     CDS             complement(3541..3924)
                     /locus_tag="VUW49_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981816.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKEMALG"
     gene            complement(3946..4152)
                     /locus_tag="VUW49_001948"
     CDS             complement(3946..4152)
                     /locus_tag="VUW49_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981817.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(4139..4300)
                     /locus_tag="VUW49_001949"
     CDS             complement(4139..4300)
                     /locus_tag="VUW49_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981818.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(4646..5602)
                     /gene="lytA"
                     /locus_tag="VUW49_001950"
     CDS             complement(4646..5602)
                     /gene="lytA"
                     /locus_tag="VUW49_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET7981819.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(5978..7348)
                     /locus_tag="VUW49_001951"
     CDS             complement(5978..7348)
                     /locus_tag="VUW49_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7981820.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIILQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQSLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            7622..8375
                     /locus_tag="VUW49_001952"
                     /pseudo
     CDS             7622..8375
                     /locus_tag="VUW49_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8448..9189)
                     /locus_tag="VUW49_001953"
                     /pseudo
     CDS             complement(8448..9189)
                     /locus_tag="VUW49_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2980 a         1830 c         1522 g         2857 t
ORIGIN      
        1 tattatatat gcacagacat atgaaatctt tttgagacag cagttatgat atatttatcc
       61 attcacaaca tcttctgtac caatactaca atattggtac attttgatta cacaatcgtt
      121 tcaaaaaata tgatggaagc ctgagtattt tatagtaact cccttcctat caactagatc
      181 aaccagttga gtgttgaatg ttgaaacacc ctagcctcag caaaggacta tgagcagcat
      241 ttcctagcca ataggacgct ccaaatcacc tattctaaat ccatcactag cttggtcagt
      301 aagatacaga ctgcaaggcg agactgacgt ggttgatgag attttggaag agtattacaa
      361 tcatagattc accagtagac ttgaccgcag atttaactat agtagattga aactagaata
      421 gtacacttct gcttctaaaa cattgttaga aatcgatttg actgtcctga tcaatttgtc
      481 ctgttctcgt ttcattctac tatactaaaa aaccacctga aacaaatcaa gtgactagag
      541 gataaaaacg aagactgggc aaaaagtttt gaaaagcaaa aaacgcataa tatcaggtct
      601 tgaaaaacct tgatactata cgtttgattg tgggaagatt gacttcattt tctcctgaaa
      661 ttgaagtttt ttctagtttc cagatataga cgaattcaat atagccatct cgctacaatt
      721 attttaacca tcgagaaaat ttttcagaat ttcaaaaatt tcattttgat ccaatgaaat
      781 gtcattattt tgaagaatag ttgacttcac aggctctaat tctatcacaa agcggtgcat
      841 gtctgctttt accgcactac ttgatgttat agccgataac ttttcatctc ctagcaagga
      901 agctgtcaaa cgggtaaggt cattcaaatg ctttttaatg gacttactta aaccttgagc
      961 acctgtggca gagcgctccc tcatatccag ccaagcttta gccttaaaaa taattaatac
     1021 tcaatgaaaa tcaaagagca aactaggaag ctagctgcag gctgctcaaa acactgtttt
     1081 gaggttgtag atggaagctg acgtggtttg aagagatttt cgaagagtat agaccacaat
     1141 tactcaatac cgaatacccc tgaatggttt ctttttcatg caccaatata ttataataat
     1201 cttcatccaa taataaggct gataaactag catcttggtc aaaatgtaag ggaatttctc
     1261 gaccgtcctt ctttaatgga tattctggta agatactaaa tagttcaatc atagaaggaa
     1321 actcaggatt agttgttgta aatcgaaaaa gctgcgcttt ctcatctttc tgacttcctt
     1381 gatactctcc caattctaaa aaatgattca aggtagtata aaattcctta ttttttactt
     1441 catcaatgat gaccatatca taatcttttg ttgtgcgact tttaaatcct tgcgaatcca
     1501 atacgataga ggtagcagtt cccccaatca gaacataata gttctgaaaa tccgcaaacg
     1561 tttcttgaaa aataactttc gtattagctg gcatcatctt ctcccagata ctgtaatatc
     1621 atattttcta gtgcttcact ctcttcctct atacgtgggt catcatcatc ttttaaggtc
     1681 aaataaagag aaatcggatc tacaaattgt ttatcatgat tatttttaaa atcattccaa
     1741 aactcagata caaaaggacg atatttccat atctctagca tctttccttt taaaatatgc
     1801 tgagaaaatg gcaaggataa ctgattgaat tttctctgcc atatgacata gctagtattt
     1861 tcatccgttt cagctaaaaa agttgaatgc gacaaagcat aagcaccacc atatagaagg
     1921 ttagaaacag attttatctg ctttatatcg ccatctggca ataaaatccg ttttttgatg
     1981 ggattaaata aacatgacac ggattttaag aataattctt tctttgacac cgtatatgtg
     2041 taaagcttat tttgcttgtt taaccaatat aaagctttaa aagtcctcaa acacctataa
     2101 attgttgagt ttggaagtcc agtgacttgt gaaagcaaat caacatttac tactttttga
     2161 ccttttgtca ataaaaaggc aatccacgtt aattgttcgc taggtgttaa ttccttaggg
     2221 atttcagtat cattcgcatt gagtactagt cccaatggag ggaagaagag gtttccctta
     2281 aagtctacaa acggaactct agcttgaagt aattgctttt tttcactgtc tgataacttc
     2341 gaaaacacca aaacaacatc catattggct ttttcaccca tagtgcgagc ctgagtaaca
     2401 aatgaactca aactccccct tctcttttct ttaatcaata aaaatgattc cttatgaaaa
     2461 gtcatttgag tatactcaaa cctttttaca aaactgattg gtaaagtcaa agtcagttcc
     2521 tcaatactga catcaacata aaaggattga aaaattgaca ttatcttttt gacattcatg
     2581 ttttttcctt attctatcat ttttattata ttttatcatt cggtataata aaatataagt
     2641 tttttgataa aataaccaaa aactactcgc tatcccctct cacattaccc tacatatcgt
     2701 ttgacatgcg actgatagtt caggaaaact tcaaggagaa cttttctctc atccactatg
     2761 caggacttac tatttcactt ctactcctat aggctcaatt tgacactttt ctttttcgaa
     2821 ttgctcatat gcctcctgaa tagtgagttc gatttatcca aatttatttt tgtctatttt
     2881 gatgaatagt tccacgaaga tagtctgaaa atgaatttgc accaattttc cttctctttt
     2941 taagcatatt attccagata tgattgccct cttattcagt aataaattac gctttaaacg
     3001 cttcaatcag tatatctcct gttgtcatat attctaaaga aaattcttct acatatgatt
     3061 taacatctct taggttatta cttcctaata tcccattatg ctttttcgct aaggaaatag
     3121 aagtcacttc tcccttacca ataatcttgt tactatcatg atttcttgtt aaatctctat
     3181 ataatgcgta ttcttcagtt ccaatgtcta tgctcacaat ctcagctgaa cccttagcta
     3241 ccaactgatc tatcctagat tttaaatggg gaattgtagg tatattgatt tcatcataca
     3301 cctcttgtgg aataacaatt ttacccaaat agagcttttc taaaagatgt tcagtgccaa
     3361 cccataaaaa tactgaaata caatctgcat caataaatac acgactagtc aaacgacaac
     3421 tcccccctct tcatctagcc catatacaat atcatatctg aaagcatcta gtaacagttc
     3481 ctcatacttc ccttgcgaaa ttctgttatt ttctaaaagt tgttcagttg aattaatata
     3541 ttatcctaat gccatttctt ttttactttc tgacaaagga cgatataaac ttgtatcata
     3601 gcctaatctt gaagctgtct ctataacact aatatccata tttttaattt cttctgcatc
     3661 aaggtatcca tcattcctca atctatataa catagcttta tgactgatac cataaaactg
     3721 acccaatttt ataatatctt ctacttcaag atgagttcta ttggcatttt ctctgatttc
     3781 ctcaaccatc ctatacagtg aagatgggaa aattaaaaaa taagaagcaa actgatccgc
     3841 ttttctttca gtttcatctc cttcaccaat caagataaga ctgactgaac tcttcttcac
     3901 ctcatcataa taaagatgat acagttcatg tgctaaagaa aatctttacc ttcctaatgg
     3961 catgtctgaa ttgactgcaa tgagactgaa ctgagttcct ttataacaga ctccgctaat
     4021 attctttccg agtccataaa ataccagcgt caaattttct atcttttgta ccaatttaaa
     4081 aatatctatc ggcgattcac catcagctcc caactttttt ctaagatttg aagctttatt
     4141 acttaaatcc attcgtgata ttcctttcac ctttttccat tttggcaatc atgctttgat
     4201 tcaaagacaa atactcagca atcttatctt gagtaagatt tgaagatatt ctaagttctt
     4261 ttattctttt tccaacatca catacattta tcatattcat acacctctca taaaaagaat
     4321 agcacacttt tgtcataatt ttttaaataa aaaaattatg acaaaacaag gaagcaattt
     4381 attgatgctg cttaaaaatc taaaattgat gagttcctat caaaaatcgt atcttcaacc
     4441 tcaaaacagt acttaaagct atccgactcg gtttacattg tcaaatttag attttatttg
     4501 agcataactt tctagtttgc tttttgattt ttgtttaata tagtagcaaa cattgaagga
     4561 aatatctcca caagaaaacg catactatta agctttttca agacctaata atatgcgctg
     4621 ttctgatttg aaagacattc cattattatt ttactgtaat caagccatct ggctctactg
     4681 tgaattctgg cttgtctgcc agtgttccgt ctggtttgag gtagtaccag cctgttccgt
     4741 ccgctgactg gataaaggca tttgatacca tggcgccttc tttagcgtct aagtagtacc
     4801 aagtgtcctt gtacttgacc cagcctgtct tcatggcacc ttcttcgttg aaatagtacc
     4861 acttatcagc gattttcttc cagcctgtag ccatttcgcc tgagttgtcg aaccagtacc
     4921 agttgccgtc tgtgtgcttc ctccagcggt ctgcaagcat atagcctgaa ctgtcaaagt
     4981 agtaccaagt gccattgatt ttctcaaact tgtcttttgg ataagagccg tctgaatgta
     5041 cgtaccagta gccagtgtca ttcttctgcc agcctgtttc aatcgtcaag ccgttctcaa
     5101 tatcatactt aaactgctca cggctaatgc cccattttgc caagtaaggg tatggatcca
     5161 catggtctga gtggttgttt ggttggttat tcgtgcaata ctcgtgcgtt ttaattccag
     5221 ctaaactccc tgtatcaagc gttttcggca aacctgcttc atctgctaga ttgcgtaaga
     5281 gttcgatata aaggcggtag tccgtcatga actcttcttt agttgaatgg ctttcaatca
     5341 gttcaaccgc tgcataggtc tcagcattcc aaccgccccc aacgtcccag gcaccattat
     5401 caacaggtcc tacctgcatg atgcaaccgt tcccaacaat gtgcgagaaa aaacctaatt
     5461 ctgggtcttt ccgccagtga taatccgctt cattctgtac ggttgaatgc ggattcccag
     5521 ttgagtgtgc gtgtacttgc ctatatggtt gcacgccgac ttgaggcaaa tctgttctta
     5581 atttactcac attaatttcc atattctact ccttatcaat taaaacaact cattttttac
     5641 aatccagaac cagaaactcc tttatttcta ccttacaaag aagacaatct tagtcacgat
     5701 taggcttgta gatagaacct caaaacgcac tattttgaca ctgtaaatag gactgacaag
     5761 gtctgcattc tatctacaat aacaccccag actaaaaagc ttttcaaaag tatattttta
     5821 cagtctctat atgtcctttt cataaatact atactttatt atatcatata aaagaagtca
     5881 aaagtctgtt aaactatttt caacaccaaa ctaaagaaga gaacacaagt tttttcgatg
     5941 ttcactagag gaaatggatt ttattcagta aatccaacta ggattgcact ttggttgcca
     6001 aaattgcctt tccttctttt atcaagggat gacggaatag tgagaagtac agttgaactg
     6061 tcatggcaac ccagataaga ggttcacaaa ggataacacc cttatatcct gcccaaggaa
     6121 taatcaaaac cacaaaaacg atttttccga ttagttcaat aaagctagaa actagaggaa
     6181 ggatcttctg ccccaagccc tgcaagcaat tgcgataaat caacaagagg ctcaaaatgg
     6241 gataaaaggc tgaactgatt tgcagataga gacttccatt ttctatcaag taaccatctg
     6301 tcgaactagc caaaaaggaa accaaggctg gactggcaaa aaagaggaaa atacaaacaa
     6361 aaactgccca ggatatactt aaacgactgc cgattcgaag accttgaaca atgcggtcag
     6421 gtcgcttagc tcctagactc tgagaagcaa aggtcgtcat tgatgcagaa atagcggtca
     6481 taggaagaag ggcaaaggtc ataatgcgtc gagctgccgt ctgggcacta ataatcactg
     6541 caccaaatgt attaacagaa aactgtaaaa tcacactgcc gatagataca attgaactca
     6601 tcaagcccat agccaaacct tgctccaaga gatccgcgta caagcttttg tcccatttga
     6661 aatgtttaaa ctgtggcaag agttctggca cacttttacg aatataataa aagcagagaa
     6721 ccgctgataa accttgcaaa ataatggtag caagtcctgc ggattgaact cccagatgca
     6781 attgcgtaat aaaatagaga tccagaacca cattaaccaa ggcagagaaa atcagaaatc
     6841 ccagggctgc tagactgtca ccaatagacc gcaacaagcc tgcaaaaaga ttataagcaa
     6901 agctgacacc tacacaggtc acaatcatag aaatatattg ataagattga ggaagaattt
     6961 ctgcaggagt atctaagtat tgcaagagag gatacaagcc aagaaagccc agcaacataa
     7021 ctagaatgct caaaagagca cctaaaatcc aagtggctgc tactgcttcc ttgattttag
     7081 tgaaattccg agccccataa taacgagcaa tgacaatccc catgccattg ccaacaccaa
     7141 gtgtaaaacc tacaatcagg tcaaaaatcg ctgtcgtcgc tcctactgca gccaaggatt
     7201 cttgaccaag aaatcgtcca acaatcaaga catcagcagt gttatagagc tgttgaaaaa
     7261 tatttgatag caagattgga aaggtgaagc ttaagagcga gggaagaatc ggaccatgta
     7321 tcaggtccac tgttcgtttt ttattcataa agctattata tcagctttcc agaggagcga
     7381 caagaaacag caaactttgc acgagctgag aaccagagca aagcaacgaa aaagcagtgg
     7441 tttttctggc tatataatat ttgtagtagg taaatcctat ttttcaaact gtttttaaga
     7501 actttttgaa agataaggac aaaattatga atacactgga actgccctat tcaaacgcga
     7561 aactagagta tactacagta aaatgaacca aaaatagtac acaatgtggt ataatcttct
     7621 tatgtcatat tcaatagatt ttcgtaaaaa agttctctct tattgtgagc gaacaggtag
     7681 tataacagaa gcatcacacg ttttccaaat ctcacgtaat atcatttatg gctggttaaa
     7741 gctaaaagag aaaacaggag agctcaacca ccaagtaaaa ggaacaaaac caagaaaagt
     7801 tgatagagat agacttaaaa actatcttac tgacaatcca gacgcttatt tgactgaaat
     7861 agcttctgaa tttggctgtc atccaactac catccactat gcgctcaaag ctatgggcta
     7921 cactcgaaaa aaagaaccac acctactatg aacaagagcc agaaaaagta gccttatttc
     7981 ttaagagttt taatagttta aagcacctag cacctgttta gattgatgaa acaggattcg
     8041 atacttattt ttatcgagaa tatggtcgct cattaaaagg tcagttaaga agaggcaaag
     8101 tatctggaag aagatatcag aggatttctt tggttgcagg tctaaccaat ggtgagttaa
     8161 tcgctccaat gacttacgaa gagacgatga cgagcgactt ttttgaagct tggtttcgga
     8221 agtttctctt actaatatta aacacaccat cgattattat tatggataat gcaagattcc
     8281 atagaatggg gaagctagaa ctcttgtgtg aagagtttgg gcataaactt ttacctcttc
     8341 ttgtttcaat tgactataat aacctcatta agatcatcaa gcgaaatgct aactttgaaa
     8401 aatttaaaac tagaatcctc atcgctttga acatcaaaaa ggagagaacc aaaggagttg
     8461 cgtttgatga gtttaatgag attattagtc gcttctaatt tggcgttgga gtagggtagt
     8521 tgaagggcat tgacaatctt ctctttatct ttgaggaagg ttttaaagac agtccgaaaa
     8581 atttctctgt ttccttattc tggaagtgaa aaagcaagag ctgatagtgg tgtttcaagt
     8641 cttctgaata gctcaaaagc ttgtttaaaa tctctttatt ggttaagtgc atacgaaaag
     8701 taggacgata aaatcgctta tcactcagtt tacggctatc ctgttgaatg agtttccagt
     8761 agcgcttgat agccttgtat ttatgagact ttcgatctaa ttgattcatg atttgaacac
     8821 gcacacgact catagcacgg ctaagatgtt gtacaatatg aaagcgatca agaacgattt
     8881 tagcattcgg gagtgaaaca gtctgggaga ctgtttcagc ctgagcctag aaatttgaaa
     8941 gcgaagctgt ttagccaagt catagtaagg gctaaacata tccatagtaa tgattttgac
     9001 gcgacatcgg acgggctcta tcgtagcaaa gaaagtgatt tcgaatgata gcttgtgttc
     9061 gtcccaagac ataatctcag gcagatttct aaaattacac tcaaagtgga agtcattgag
     9121 ctttcgaatg acagttgaag ttgaaatggc cagctgatgg gcaatatcgg tcatagaatt
     9181 cttttcaat
//
LOCUS       DAVDWM010000065         9031 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVDWM010000065 DAVDWM010000000
VERSION     DAVDWM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.065"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(208..1176)
                     /locus_tag="VUW49_001954"
     CDS             complement(208..1176)
                     /locus_tag="VUW49_001954"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET7981821.1"
                     /translation="MSFSDLKLFALSSNKELAECVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1321..2136)
                     /locus_tag="VUW49_001955"
     CDS             complement(1321..2136)
                     /locus_tag="VUW49_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET7981822.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLLGWLSSFANTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDVG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2161..2658)
                     /locus_tag="VUW49_001956"
     CDS             complement(2161..2658)
                     /locus_tag="VUW49_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7981823.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESTLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            complement(2731..4092)
                     /gene="radA"
                     /locus_tag="VUW49_001957"
     CDS             complement(2731..4092)
                     /gene="radA"
                     /locus_tag="VUW49_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7981824.1"
                     /translation="MAKKKAIFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELIQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4106..4621)
                     /locus_tag="VUW49_001958"
     CDS             complement(4106..4621)
                     /locus_tag="VUW49_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7981825.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQAQKLSK
                     TPLLSSAEIIVSSAVTRALETASYVVCATGLSLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMFMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4623..5066)
                     /locus_tag="VUW49_001959"
     CDS             complement(4623..5066)
                     /locus_tag="VUW49_001959"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7981826.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5123..5220)
                     /gene="ffs"
                     /locus_tag="VUW49_001960"
     ncRNA           complement(5123..5220)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VUW49_001960"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5253..5720)
                     /locus_tag="VUW49_001961"
     CDS             complement(5253..5720)
                     /locus_tag="VUW49_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7981827.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK"
     gene            complement(5921..7207)
                     /locus_tag="VUW49_001962"
     CDS             complement(5921..7207)
                     /locus_tag="VUW49_001962"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7981828.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7438..7674)
                     /locus_tag="VUW49_001963"
     CDS             complement(7438..7674)
                     /locus_tag="VUW49_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET7981829.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7940..8801
                     /locus_tag="VUW49_001964"
                     /pseudo
     CDS             7940..8801
                     /locus_tag="VUW49_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8836..>9031)
                     /locus_tag="VUW49_001965"
     CDS             complement(8836..>9031)
                     /locus_tag="VUW49_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981830.1"
                     /translation="RFHRMGKLELLCEEFGHELLPLPPYSPEYNPIKKTWAHIKKHLK
                     KVLPSCNTFYEALLSCSCFN"
BASE COUNT         2895 a         2157 c         1449 g         2530 t
ORIGIN      
        1 cctactagaa ttctccttag aaagcgtcga ttcaagtgct atcactgttc aaaaatgatg
       61 gtcgctgaaa cttctgatga cgtacagtca tatttcttct ctttttatta tatcacagtt
      121 ttaaatctag ctttactaga ttcaccgcta ctatctattt attcggaaaa aagacgaaaa
      181 acctgagaat catctcaggc ttggtcatta aatttttttc tcaatatcga aaagtggaga
      241 aagtggtcgt ttttcatgaa tacgtacgat agcatcccct aggagatgag cgattgaaat
      301 ctgctcaatc ttatcaatca aacgctcttc tggcagatag atggtatcca aaacaaccaa
      361 tttcttaata gctgattttt ggatattgtc cgtagcagga ccagaaagaa ctgggtgcgt
      421 acagcttgca tagacttcaa cagcaccagc ttccgcaaga gcatctgccg catgacaaat
      481 cgttccagcg gtatcaatca tatcatcaat caagatacaa gtcttgcctt caaccttacc
      541 gatgatgttc ataacttcac tagtattcat cttatcaacg ctacgacgtt tatcaataat
      601 agcgatagat gttttcaaaa attctgccaa cttacgagca cgagtcaccc ctccatggtc
      661 cgggctgaca accacatagt cagaaccaac cataccacga cgctcaaaat aatctgcaat
      721 cagaggagca cccatcaaat gatccacagg aatatcaaag aatccttgaa tttgcgcagc
      781 atgcaagtcg atggtcaata aacgatccac tccagctact tcaagcatat ttgcgacaag
      841 ttttgaagtg attggctcac gcgctctcgc ctttctatcc tgacgtgcat acccatagta
      901 aggcatgaca acattgacag attctgcact cgcacgcttc aaagcatcta ccataatcaa
      961 aatttcaagc agattgtcat ttacaggcga actagttgat tgtaggataa agacgtgttt
     1021 cccacggatt gattcttcaa tgttgacctg aatctctcca tctgaaaatt ggcgaacact
     1081 tgatttcccc aactctatcc caatctcctg cgccacacat tctgccaatt ctttattaga
     1141 agaaagggca aacagcttta aatcagaaaa agacatgatt tcctccggta tatatgtatc
     1201 acttgtgctt ttcacaagat tttccatcta ccattgtagc gctttttgca ctatttttca
     1261 atcaaaaata aaagaagggc accatatttg tacccttgca tcattctttt gaaaaatatt
     1321 ctaggtcatc aactcattgt gtttctcaac aaagcaataa gcatgataaa aaccatagag
     1381 agcaatagcc gtaaccactg gaatcgctaa aggcaactct gtttccaact ccacaaaagg
     1441 agagttaaac aagaagtgag ttcccaaggc taaacctaga aaaataaggc cctgtttctt
     1501 gccaaccttc tgtcctttat aggctctgta aagcaagtaa acacctacta cagctagacc
     1561 tgaaaaagtc cagtgagagg caattcctga gatgatacgc tctaaaattc gcgaaatagt
     1621 aaagtcaaag ccctctggca aatccgtacg aatataacca acatcctcaa tcatttggaa
     1681 tcccaaaccg gaagcaattc caagtaaaaa taaagatttt aattttcgca caggaatcaa
     1741 agccaaaaca aaaacaagtg gcaataattt caagggttct tctaccaaag gagccacaat
     1801 agcactttca aaggcattta aaaatggact atctgggaaa agaaccccca gtaaatcatg
     1861 gatataagta ttagcaaaac tagacaacca gcctaaaagg aacatccctc ccaataaaga
     1921 cagaatcaaa accttctttg gcaattccca ttttttccca atacggaaga gaaaataaag
     1981 agccggaatc atgtaaaaga gagctagaaa gatagaaact cccattagtc catattccgc
     2041 acctgacctc gaaccgtccg tatagtagat ggtttcatac tgtaaaccaa tacatagcaa
     2101 taaaataaaa ataaataaaa tactgctttt cttcatacac tttctttcta aatgaagtat
     2161 ttataattct acgactgcca tacttcctgt atcaacattg taaatggcac cagagataat
     2221 gacatcgtct ggtattaggg tagactcgat aagcagttgc atatcctcgc gtacactctc
     2281 ttctatatct tggaagggca agaagtcctg gtctgacaca tctacaccta attcctcttt
     2341 taaatactcc tgaaaaggtt cattttcaaa ggtctgagca ccacagtctg tatggtgcaa
     2401 taccacaatc tctcttgtcc ccatttgttg ctgggaaata actagcgaac gaatcatgtc
     2461 ttcagtcact cgaccacctg cattccgcaa gatatgagca tccccaagtg ccaagcccag
     2521 agcttgcgca acgtgcagac gagagtccat acaggtcaca atagctactc ttgttttggg
     2581 tttaagtggc agatttaact gcccatgtag ggcaacataa gcctgattgg cttgcataaa
     2641 ctgttcaaaa tacgacacga ttccctcctt gaaaatttga tagtcaaata tttctcctat
     2701 cttatcattt ttaagagaat ttgtcacgga ttatgcaaag acctttttca agacttcctg
     2761 aatcgttgtc acgccaatga cctgaatttc cttaggcaga gtgattcctg tcaaggaatt
     2821 cttaggtaca taaatcttag taaagcccag tttagcagct tcgttgatgc gttgctcaat
     2881 acgattcacg cgccgaatct ctcctgtcaa gcccagttct ccgacaaaac attcctgagg
     2941 attagttggc ttgtctttgt agctcgaagc aatagcaact gcaacagcca agtcaatcgc
     3001 aggttcatcc aatttaacac caccagcaga tttgagatag gcatcctgat tttgcaagag
     3061 aagccctgcc cgtttttcca aaacagccat aatcaagcta gcacggttaa aatcaagtcc
     3121 tgtcgtagta cgcttggcat ttccaaacat ggtcggtgtt accaaagcct gaacctccgc
     3181 caaaatcgga cgcgtccctt ccatggttac aacgatggag gaaccagtcg ccccatccaa
     3241 acgctcttct aggaaaactt gactcggatt gagtacctca accaagccgc ccgactgcat
     3301 ctcaaaaatc ccaatctcat tagtggaacc aaaacgattt ttgaccgctc tcaaaatacg
     3361 aaaggtgtgg tgacgctccc cttcaaagta aagcaccgta tccaccatat gctccaacat
     3421 acgaggccca gccaaggttc cttctttggt cacatgacct acgataaaga tggcaatgtt
     3481 attggtcttg gccaactgta tgagttcagc ggtcacttca cgcacctgag aaacagaccc
     3541 ctgcacccct gaaatctcag gagacatgat ggtctggatg gaatcaataa tgagaaagtc
     3601 tggctggata cgctccactt ctgcacgaac actctgcata ttggtctctg catagagata
     3661 aaactcacta tcaatatcac ctaagcgctc tgcacgtagt ttaatctgct gggcagactc
     3721 ctccccactg acatagagaa ctgtccccac ttgggacaac tgggttgaga cttgtaggag
     3781 aagagttgat ttcccaatcc caggatcccc accgatgagg acgagacttc ctggtaccac
     3841 tccgcctcca agcacacggt tgaattcctc catctccgtc ttggttcgat tgacattgat
     3901 ggaagtcacc tcagctagtt tcatgggctt ggttttctca cctgtcaagg acacacgcgc
     3961 attcttgacc tcagcaacct caacctcttc cacaaaagaa gaccaagacc cacagttggg
     4021 gcaacgtccc agatatttag gggaattata cccacaattt tgacatacaa atatcgcttt
     4081 tttctttgcg atgacaaacc tctttctata tctctaactc acactcaatc acttggcaaa
     4141 aatcaatctt ctcatttggc acaaactggc gcatgaacat tctatgagca acaactacca
     4201 cagtctgatg ttctcgatac ttagacatac attctagaaa ccgagacttc atttccgtag
     4261 ctgtctcata ttgaatagga ctattaggaa gcaactcccc cttgttttct aaaaacagtc
     4321 ttctagctgt ttcaaagttt tctattcctg ttttatagac ctgccattca tgtaataaag
     4381 gctctactct taaagaaaga cccgtagcac agaccacata cgaagctgtt tctaaagctc
     4441 ttgtgactgc agaagatacg attatttcag ctgacgagag taaaggagtt ttgctcaatt
     4501 tctgagcttg ctgccgtccc atctcagaca agggtgccaa atctatccca aatcctatat
     4561 aagaacgctc ctctaactca cggtaatctg gctccccatg acgtacaaag ataatcttca
     4621 ttctagtgcc ctgtcgatcc aaatccacca gttcgaacgc catcagctgc atctccatct
     4681 gcaattaaga aagtagcaaa aacagcctgg acaatacgct ccccaacttc aagaacaacc
     4741 tcttggtctg tgatattctt catctgcgca aaaatatgcc cttcatttcc aggatttcca
     4801 taataatccc catcaatgac cccaactgag ttaattaaaa ccaagccctt cttacgagga
     4861 tttgaagaac gatcatagag gtagagaacc tcagtcggct gcatataagc cttaacccct
     4921 gtcggaacca agacaatctc tcctggcgca acaactgtac gcacagcaac ctttaagtcg
     4981 taaccagccg catgcgctgt ctcacgcttg ggcaataaat tttcatctgt aaaactcgaa
     5041 accaattcaa aaccacgaat tttcataatt ttctcttttc tattatcatt tattctagat
     5101 tattctatct tatttattcg gaaaaagcac gaaaaaaaga gcacacaatt caaatcgctt
     5161 agggctgctg gattcctccc ctgacccgct tcacgcagaa ctgttgctcc actatttatt
     5221 atatcacatt cctattcatt ttaaaagcaa aattattttt tccgtctatt tctaaaaaag
     5281 tcctgcatga tagctgcaca ttcatcttcc aaaattcccg tttcaacctc tacacgatgg
     5341 ttaagacgct catctgtcaa gatatcgtac aaacttccag cagcgccaaa tttctggttt
     5401 ttagccccat agaccacatt tggaatacgg gcaagcccaa tcgccccact acacatgaca
     5461 caaggttcaa tggtcacaaa aagtgtgcaa tccagcaagc gccagctctc ctcactcaag
     5521 ttcgcatcct ctatagccat aatttccgca tgcataaccg ctcgctgtaa ttcctcacgc
     5581 gcattatgcc cacgaccaat gatttcccca tctttgacaa tcacacaacc aattggaatt
     5641 tcatcgtgtt caagagcaat ctcagcctct ctcaaagcct ccctcataaa gacttctttt
     5701 tcttcaagcg tataatacat ctcttttctc ttttcctact tatcgatttt attattatat
     5761 catgaatccc aacacaaaaa aagccaccga atgcggtgac tctataggga gattattatg
     5821 aaaaagaaaa gtttaggata tttgttacaa caagttagga ggtcttcttg taactgtcta
     5881 tagtataccc gacctatctt aaacaaatct taaaaatctc ttaggaccaa acactttcta
     5941 aaatatttgt ttgttcacga ccaggaccta ctgagaaagt agaaatacga acgccaacca
     6001 attcactcac acgacgaaca tagttacgcg cattctcagg aagatcttcc aaattgcgaa
     6061 ctccggtaat atcttctgac caacctggca actcttcata gataggcttg caacgtttca
     6121 attgctcaag actagctgga tagtagtcaa tacgttgacc gtcaagatca taggccacac
     6181 agattttcac agtatccaaa ccgctcaaaa catcaataga gttcaaagaa aggttagtaa
     6241 taccagaaac acgacggcta tgacgcatca caactgagtc aaaccaacct acacgacgtg
     6301 gacgaccagt tgttgtacca tattcatgac ccacttcacg gatacgttct cccacttcat
     6361 caaacaactc agttgggaaa ggaccatctc ctacacgact cgtataagct ttacatacac
     6421 ctacaacctt gtcaatcttg cttggaccga taccagaacc aattgtcaca ccaccagcta
     6481 cagggtttga tgacgtaaca aatggataag taccttggtc gatatctagc ataacacctt
     6541 gtgcaccttc aaaaagcaca cgtttgccat tatcaagcgc atcattcaag ataacagatg
     6601 tatctatcac gtatttcttg atttgttgac catattcgta atattcttca aaaatatcat
     6661 cgaaaacaat cgctttactg tcatacaatt tttcaaaaag acgattcttt tcagcaaggt
     6721 tacgttctaa acgctcacgg aaaatatctt tatctaaaag atctgcaata cgaattccaa
     6781 cacgagcagc cttgtccata taagctggac caattccctt aattgtcgta ccaatcttat
     6841 tgtcgccctt agcttcttct tgcaagcgat ccaactcgat atgataaggc aaaataacat
     6901 gcgcacgatc agaaatacgc aagttatcag ttgttacacc ttcctcatga agatagctca
     6961 actcttttac aagagattta ggatttacaa ccataccatt cccaatgaca gatatttttt
     7021 cagggaagaa aatcccagat ggaatcaagt gcaacttaaa tttcttaccg tcaatcacaa
     7081 tcgtgtgacc agcattatca ccaccttggt aacgtgcaat cacttctgca ttcgctgaaa
     7141 ggaagtctgt aatcttccct ttaccttcat caccccattg ggtacctaca acaacaactg
     7201 aagtcataat tttgtctgag ccctcaggct cttcctttct cacatacatg gcaggactct
     7261 cacctgcaat tatatcttac aatttattat aataaaaaat cgccttttta tcaagaagaa
     7321 acaatagaaa gatttgctat ttccaactat taaaaaatga tttagaaaaa ttactagcta
     7381 tttactatta tctttccata aaagagtaaa ttagttcgga aatttactaa aattacctca
     7441 acaagaaata aacccccgat tcattaccaa tttttcaaga tacaaacgat aagcaacacg
     7501 ataatggtaa acgataaaat ccctacgaca accaatgcca tatctcacta aataataaat
     7561 taaaaattta aaatgaacat gttcccagtc aaaattatca ccaaatgtag gaccatactc
     7621 ttcttcaata ctatcataga aattagccat ctgctcataa attttttgta acataatcaa
     7681 atactccttt tcttttttat aaacttattc taacaaaaaa ttttacacat tcactatcaa
     7741 ttcctgaatt gttaaaatac cagcctcaac aagataaaaa taggaaaagt tgacaaaata
     7801 gaaacaaatt tgctacctaa atttcaaaca attaaagttc taaaaagacc gccttaaaac
     7861 ttttgggtaa atcctattaa aactataata tttttatata ggtactatgg ttctgatttt
     7921 ctagtataaa aaaattcaaa ctagaattct ccttagaaag cgtcgattca agtgctatca
     7981 ctgttcaaaa atgatggtcg ctgaaacttc tatcgtcaag aagaatcacc aaatccctcg
     8041 tattatcaac caaaagattg ctcaaaagtt aattgaaaag atttctatga ccgacattga
     8101 tcatcagttg tctatttcaa cttcaactgt cattcgcaag atcaatgatt ttcactttga
     8161 gcatgatttt tcgcgtcttc ctgagattat gtcctaggac gttgaaacag tccggggagt
     8221 gactgtttca atcgggagat ggagatgagc tttattgctc aggactttga taagctcaat
     8281 atcataactg ttcttgagag cagaacacaa gccatcatcc gaaatcccat ggatacaagg
     8341 ctatcaagcg ctactggaaa ctcattcaac aggatagtcg taaactgagt gataaacatt
     8401 tttatcatcc tacttttcgt atgcacttaa ccaataaaga gattttaaac aagcttttga
     8461 gctattcgga agacttgaaa caccactatc aactctatca gctcttgctt tttcacttcc
     8521 agaataagga accagagaaa tttttcggac ttattgagga caatcttaag caggttcatc
     8581 ctatttttca gactgtcttt aaaaccttcc tcaaagataa agaaaagatt gtcaacgccc
     8641 ttcaattacc ttattccaac gccaaactgg aagccaccaa taatctcatc aaacttatca
     8701 agcacaatgc ctttggtttt aggaactttg aaaacttcaa aaaagaaagg acgaaatttg
     8761 tcctttctaa atcttcgctt tcttcaaccc actacagttg acaaagaacc gaaaaatgtc
     8821 tcgcctctaa tatagtcaat tgaaacaaga acaagacaaa agagcctcgt aaaaggtatt
     8881 gcaacttggt aatacctttt tgaggtgctt tttgatatga gcccatgttt tcttaatagg
     8941 attgtactca ggtgagtagg gaggaagagg taaaagttca tgcccaaact cttcgcataa
     9001 aagttctagc ttccccattc tatggaatct t
//
LOCUS       DAVDWM010000066         8902 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVDWM010000066 DAVDWM010000000
VERSION     DAVDWM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.066"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            132..449
                     /locus_tag="VUW49_001966"
     CDS             132..449
                     /locus_tag="VUW49_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7981831.1"
                     /translation="MERQTLKDIEVIIVNQSTEEIASISQQMNLSFNIKIIDENSFAE
                     LSDMITGDYITFLSSNDSLFDWTFEKMYHAIKLSDSDVVLGHFADLVDGVFYFYPWGG
                     IIG"
     gene            446..1450
                     /locus_tag="VUW49_001967"
     CDS             446..1450
                     /locus_tag="VUW49_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HET7981832.1"
                     /translation="MTENLTNVQVLQKMDDTDHRIRKQYRSLFGKLFNSKLLRKINQF
                     DINNLIWRLYIHSKTATYINFPTYIYKPVIDAKPLKDSYKIILENYENRIKDCAVLEN
                     YDIERAKKQYIQELANFSAWLKNDGEYLDSDIVYHKLIAAEKGIFPFLELDRLDFTII
                     SNNCVGGLIYKQLGFQYRTPFVGLFILPDDYYKLTKHFRYYMEQELVFEEELISPSWT
                     HEVYPLGHLGDIDIHFLHYSSIEEARTKWEKRKKRINWNNLYFKFDNKDSASEEILNK
                     MDNLPYSNKLILVNRPYKNLKSQCVIPGQEHLPELAIMSDPLNTFDIIAWLKKGGNAL
                     "
     gene            1552..3681
                     /locus_tag="VUW49_001968"
     CDS             1552..3681
                     /locus_tag="VUW49_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET7981833.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            3668..4117
                     /locus_tag="VUW49_001969"
     CDS             3668..4117
                     /locus_tag="VUW49_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET7981834.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            4175..4447
                     /locus_tag="VUW49_001970"
     CDS             4175..4447
                     /locus_tag="VUW49_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7981835.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(4495..4659)
                     /locus_tag="VUW49_001971"
     CDS             complement(4495..4659)
                     /locus_tag="VUW49_001971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981836.1"
                     /translation="MDEAGEILLILFKNQSANYINFTLPTKLQPAAGFLVCTLIFIEY
                     KSKKALNQAV"
     gene            complement(4801..4992)
                     /locus_tag="VUW49_001972"
     CDS             complement(4801..4992)
                     /locus_tag="VUW49_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981837.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            5420..6178
                     /locus_tag="VUW49_001973"
     CDS             5420..6178
                     /locus_tag="VUW49_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981838.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPTRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            6180..8168
                     /locus_tag="VUW49_001974"
     CDS             6180..8168
                     /locus_tag="VUW49_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981839.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(8210..8851)
                     /locus_tag="VUW49_001975"
                     /pseudo
     CDS             complement(8210..8851)
                     /locus_tag="VUW49_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
BASE COUNT         2745 a         1617 c         1810 g         2730 t
ORIGIN      
        1 ttgtgctata attaaataaa aagaaaatca ttatcagaaa attatgggag gtagtcaaac
       61 tgacttactc aattagtgtt gttttgttac atcaaggaga taaaaatagt ttattagagt
      121 ctttgtcaat agtggagaga caaacattaa aagatataga agttattatt gtaaatcaat
      181 ctacagagga gatagcttcc attagccaac agatgaatct atcatttaat attaagatta
      241 tagatgagaa ttcttttgct gagttgtctg atatgattac aggagattat attacgtttc
      301 tatcttcaaa tgattcatta tttgattgga catttgaaaa gatgtatcat gcgataaaat
      361 tatccgattc tgatgttgtt cttggtcatt ttgctgattt ggttgatgga gtattttact
      421 tttatccttg gggggggata attggttgac agaaaattta acgaatgttc aagttttaca
      481 aaagatggat gatacagatc atcgaattag aaaacaatat cgctcattat ttggaaaatt
      541 attcaatagt aagcttttac gaaaaataaa tcaatttgat ataaacaacc ttatttggag
      601 actttatatt cactctaaaa ctgcaaccta tattaatttc ccgacatata tctataaacc
      661 tgttatagat gctaaacctc taaaagactc ctacaaaatc atattagaaa attatgaaaa
      721 tagaatcaaa gattgcgccg tcttagaaaa ctatgatata gaaagagcga aaaaacaata
      781 tattcaagaa ttggcaaatt ttagtgcttg gttaaaaaat gacggtgaat atttagattc
      841 tgatattgtt tatcataagt taatagctgc agagaaagga attttccctt ttcttgagct
      901 tgatagatta gattttacga ttataagtaa taattgtgtt ggaggattaa tttataaaca
      961 gcttggtttt caatatagaa caccttttgt tggtttattt attcttcctg atgattatta
     1021 taaacttaca aagcattttc gttattatat ggaacaagaa cttgttttcg aagaggaact
     1081 tatttcaccg agctggactc atgaagttta tcctttgggg cacttaggag atatagatat
     1141 tcattttcta cattattcta gtattgaaga agcacgaact aagtgggaaa aaagaaaaaa
     1201 acgcataaat tggaataatc tttattttaa atttgataat aaagattcag ctagtgaaga
     1261 aattttaaat aagatggata atcttcctta ttctaataaa ttaattttag tcaatcgtcc
     1321 atataaaaat ttgaaaagtc aatgtgttat tccaggtcag gaacatcttc cagaactggc
     1381 tattatgtct gatccattaa atacttttga tatcatagct tggttaaaaa aaggtggcaa
     1441 tgctctttaa gtgcaagtta ttgcgctgta gcatataaat ctatttccta catatttttt
     1501 aaatgttcta cgagttaatt tgaaacgttt agcttgtggt ataatagatt tatggataaa
     1561 aaatatgaaa aaatctctca ggatttggga gtgacgttaa agcaaattga taccgttcta
     1621 agtttgacag ctgaaggggc gactattccc tttatcgcgc gttatcgcaa ggacatgact
     1681 ggtagtctgg atgaggtggc gattaaggct attattgatt tggataaaag tctgacaaat
     1741 ctcaatgacc gtaaggaagc tgtcttagct aagattcaag aacaaggtaa gttgaccaag
     1801 gaattggaag aagctatctt agttgccgaa aaattagcag acgttgaaga actctatctt
     1861 ccttataagg aaaagcgtcg taccaaggca accattgccc gtgaagctgg actctttcct
     1921 cttgctcgct tgattttgca gaatatagtt gacttagaga aagaagctga aaagttcgtc
     1981 tgtgaaggat ttgcgactgg caaggaagcc ttgaccggtg cagttgatat tttggtcgaa
     2041 gccttatcgg aagatgtgac cttgcgttct atgacttatc aggaagtgct gagacactct
     2101 aaactcactt ctcaagccaa ggatgaaagt cttgatgaaa agcaggtttt tcagatttat
     2161 tatgattttt cagagacagt tggaactatg caaggctatc gtaccttggc tctcaatcgt
     2221 ggggagaaac ttggtgtctt gaagatcggt tttgaacatg cgacggaccg tattcttgcc
     2281 ttctttgcta ctcgtttcaa ggtgaaaaat gcttatattg atgaagttgt tcagcaatcc
     2341 gttaagaaaa aggtcttgcc tgctattgag cgtcgtattc ggacagaatt aactgagaaa
     2401 gctgaagagg gagctatcca acttttttct gacaatctgc gcaatctcct cttggttgct
     2461 ccactgaaag ggcgcgtggt tcttgggttt gacccagcct ttcgtacagg tgccaagcta
     2521 gctgtcgtgg atgcaacagg aaaaatgctg acaactcagg ttatttatcc tgttaaacca
     2581 gcatcagctc gtcaaatcga agaagccaag aaagatttag cagatttaat tggtcaatac
     2641 ggtgtagaga ttattgccat tggaaatgga acggccagtc gtgaaagtga agcttttgta
     2701 gcggaagttc tgaaagattt ccctgaagtc agctatgtta tcgttaatga aagtggtgct
     2761 tctgtctatt ctgccagcga acttgctcgt caggagtttc cagacttgac cgttgaaaaa
     2821 cgctctgcca tttctatcgc ccgtcgtttg caagatcctc ttgcggaatt ggtcaaaatc
     2881 gatcctaagt caattggtgt cggtcaatac caacacgatg tcagtcagaa gaaactatct
     2941 gagagtctgg actttgttgt cgatacagtg gttaaccaag ttggtgtcaa tgtcaataca
     3001 gctagcccag ctcttctttc acacgtagct ggactcaata aaaccatttc tgaaaatatc
     3061 gtcaaatacc gtgaggaaga gggaaaaatc acttcacgct cccaaatcaa gaaagttcct
     3121 cgtttggggg ccaaggcttt tgaacaggct gctggtttcc tccgtatccc tgaaagtagc
     3181 aatatccttg ataatacagg agttcaccca gaaaattacg ctgccgttaa ggaactcttc
     3241 aaatgcttgg acattaagga tctgaatgaa gaggcccaaa gcaaactcaa gtcgctttca
     3301 gtcaaggaaa tggcgcaaga gttagacctt ggtccagaaa ctcttaaaga tatcattgca
     3361 gatcttctca aaccaggtcg agatttccgt gattcctttg atgctccagt tctccgtcaa
     3421 gatgtcctag atatcaagga cttagtggtt ggacaaaagc tagaaggagt ggtgcgtaac
     3481 gttgttgatt tcggtgcttt tgttgatatc ggaattcatg aggacggtct catccatatt
     3541 tcccatatga gtcgcaagtt tatcaaacac cctagtcaag tggtggcagt tggagatttg
     3601 gtgagcgttt gggttaagaa aattgatact gaacgtgaaa aagttaatct gtctctccta
     3661 gctccaaatg aaactgactg attacgtcaa gcaggtttca ctagaagact tcggcagacc
     3721 ttttatccat catgtccagt ggaataggcg tctacgttcg acaggtgggc gatttttccc
     3781 caaagatggg catttggatt ttaatcccaa ggtttatcag gaactggggt tggacgtttt
     3841 taggaaaatt gtccgacatg aactctgtca ttatcacctc tattttcaag ggaaaggcta
     3901 tcaacacaag gatcgggatt ttaaggaact gttgaaagca gtggatggat tacggtttgt
     3961 accatccttg cccaatagta actccaagcc aatcaagctc tatcgttgcc aatcctgcca
     4021 gcaaacttat cagcgtaagc gtaggattga tacccaacgc tatcgctgtg gactttgtcg
     4081 aggaaagttg ctattagtaa atcagcctga ggactgatga aagccgagcc tatccgtgat
     4141 atactagtct aacctagcag aaagaggaaa tatcatgaac agtaagtttt ataaaatgag
     4201 acgaaatcgt atgatttcag gagttttagc aggtctgtca gacaagtgga actttgatgt
     4261 tacccttgta cgttttctgt ttgccatttt tacagtcgca aactttggac ttggggtgat
     4321 tatctatatc atcctagctt ctatcatgcc aaccaaggaa gaaatcgaag cagaaatgta
     4381 cggaacaggc ccacgtaaac gcaaagaagc tcaagccatt gacgataatg aaggctggtt
     4441 ttggtgaggt tttacaaaag atttggaata cgcaaaaagc tttaaatcaa tagtctaaac
     4501 tgcttgattt aaagcttttt tacttttata ctcaataaaa atcaaagtgc aaactaggaa
     4561 gccagccgca ggctgtaatt tagtcggcaa tgtgaagttg atatagtttg cactttgatt
     4621 tttgaatagt attaataata tttcgccagc ttcatccaca aactatagtg gattggaact
     4681 agaataggac acctatactt ctaaaaaatt gttagaaatc gatttgactg tcctgatcga
     4741 tttgtcctgt tcttatttca ttttactata aaagcagtaa tttaaacgca gttttttaat
     4801 ctatttttgc tgttgagaca atacattttt ggaaaaatgg tggatgctga taacaactga
     4861 taaccagcaa ataacataag cagtgccggt aaattctgta ctgataaagt gtgtgtgttc
     4921 aactaagagc tgaccgatga atggagagag cagatagaga agtccgatga gtatatgttc
     4981 tttgtgtttc attaaagcgc accttctttc cttataggtt gaacgacttg caagtgttaa
     5041 attgaatggt atgttatcta ggtaagctta ctaaaatgtt agttcccgct tcctcctttt
     5101 caaagtaagc cttttcacac ttatatttta caactttaaa aataggaaat caaatcattt
     5161 tctaagtaat tttgcttata tttcacccaa tcttacaaaa atgtaagatt aaagtctctt
     5221 ttgtaaggtt aggttatggc aagcggtctt tttttgatgt aaactagact cataaagaac
     5281 aaaggagtaa catcatgaaa caaatcttac ataagaaaac tagaaaacca agtcacaaag
     5341 aaattgagcg cgttcagctg ggatgcacta tgatgcaagc tacatttcat ttgatgggat
     5401 attaagaaag gagattctca tgacactttt agatgtaaaa cacgttcaaa aaatttataa
     5461 aacacgtttt cagggcaacc aagtagaagc cctcaaggat attcacttta ccgtagaaaa
     5521 gggtgactac gttgccatca tgggtgagtc tggttctggt aaatcaactc ttctcaatat
     5581 tctagctatg ttggataaac caactcgtgg gcaggtttac ttgaacggaa ctgacaccgc
     5641 aactattaaa aattcacagg cttctagttt ccggcgtgaa aagctaggat ttgtcttcca
     5701 agactttaac ttgctagata ctctgtctgt taaggacaat atcttgcttc cgcttgtctt
     5761 gtcaagaaga cctataacgg agatgatgaa gaaattggtg gtgacagctg agaatctggg
     5821 tattaaccaa ttgcaagaga agtaccctta cgagatttct ggtggccaga aacagcgtgt
     5881 agcagtagcc cgcgccatca tcacagaacc tgaaattctc cttgcggacg agccaacagg
     5941 agcccttgat tccaagtcat ctgcagcctt acttgatgtc tttaatgaaa tcaatgagcg
     6001 tgggcaaacc atcctcatgg taacccactc aacagcagct gctagcaggg ccaagcgtgt
     6061 tctctttatc aaagacggca ttctttacaa ccaaatctac cgtggagaga agacagagcg
     6121 tcagatgttc caagaaatct ctgatacctt gactgtcatg gcaagcgagg tgaattagta
     6181 tgtttcgatt aaccaataag ttagcggtat cgaacttgat taaaaaccgc aaactctact
     6241 atccctttgc actggctgtt ctcttggcag tcaccatcac ctatctcttt tactccctaa
     6301 ccttcaatcc aaagattgcg gaaatccgtg gaggaaccac cattcaagca acacttggat
     6361 ttggtatgtt tgtcgttacc cttgcgtcag ccattatcgt cctctatgcc aatagttttg
     6421 tcatgaaaaa ccgttccaag gaactgggta tatatggcat gttaggcttg gagaagcgcc
     6481 atctaatcag tatgaccttt aaggagttag tgctatttgg gattctaact gttggagcag
     6541 gaatcggtat tggagccttg tttgacaagt tgattttcgc tttcctactc aaactgatga
     6601 aattgaaggt ggaactggtc gctaccttcc agatgaaagt tgtcattaca gtacttcttg
     6661 tcttcggttt gattttccta ggcctcatgt tcctgaatgc ccttcgaatc gcccgtatga
     6721 atgccctcca gctctcacgt gagaaagcta gcggagagaa aaaaggtcgc ttccttcctc
     6781 tccaaacgat tcttggttcc ataagtttag ggattggata ttatcttgcc cttacggtaa
     6841 aagatcctct tacagcctta acaaccttct tcatagctgt tttactggtt atctttggta
     6901 cttatctttt gttcaatgca gggattaccg ttttccttca aatcttaaag aaaaataaga
     6961 aatactatta ccaacctaat aatctcatat ctgtttctaa cttgattttc cgtatgaaga
     7021 aaaatgcagt tggactagca actatcgcta ttttgtcaac aatggttttg gtaaccatgt
     7081 cagcagcgac aagcattttc aattccgcag aatcctttaa aaaagttcta aatcctcatg
     7141 attttggggt ttcagggcaa aatgttgaaa aagaagattt ggacaaactc ttgagccagt
     7201 ttgcaagtga caaaggttat aagattaaag agaaagaagt gcttcgttac acttactttg
     7261 ctgttgcgaa tcaagaagga accaagttaa ctatttttga aaaaggacaa aaccgtgtcc
     7321 aaccaaaaac agttttcatg gtatttgacc aaaaagatta tgaaaatatg actggtcaaa
     7381 aactgtctct atcaggaaat gaggtcggtc tctttgccaa aaatgacgga ctgaaaggac
     7441 agaaagctct aactctaaat gatcatcaat tttctgtcaa agaagaattt aataaagatt
     7501 tcattgtcaa ccatgttcca aataagttta atatcttgac tactgattac aattaccttg
     7561 ttgttcctga tttacaagcc tttttggatc aattcccaga ttcggctatc tataatcagt
     7621 tttacggtgg tatgaatgta gatgccagcg aagaagaaca actcaaggtc gctgaggagt
     7681 atgaaaacta cctcaatcaa tttaatgctc aattagacac agaaggtagc tatgtttatg
     7741 gtagcaatct agcagatgct agttctcaga tgagtgccct ctttggtggt gtcttcttta
     7801 tcggtatttt cctatccatt atctttatgg tcggaactgt tctggtcatc tactacaaac
     7861 aaatttctga aggctacgaa gaccgtgaac gctttattat cttgcagaaa gtcggtttgg
     7921 accaaaagca aatcaagcaa accatcaaca aacaggtttt aactgttttc ttccttcctt
     7981 tgctctttgc cttcatacat ctcgcctttg cctaccatat gcttagcctg attttaaaag
     8041 tgattggtgt actggatacg actatgatgt tgattgtgac cttgtctatc tgcgctatct
     8101 tcctcatcgc ctatgtgctg attttcatga ttacttcaag aagttatcgc aagattgtgc
     8161 aaatgtaaaa aagatacctc gacttcaaaa tcgaggtatt tcttgtattc taaatgctga
     8221 aaagttgttc gagcaggaag gtaactccca tggtcaagag accaatagca aggttccgaa
     8281 tcatagctgt tttggttggg gcttttccaa gtctagcact tgtgtaacca gtgagaagaa
     8341 gggccacacc gacaataagg acggtagcag ggatgcggta atcacttgga aaaatggtca
     8401 ctgacagcat tggaggcaaa cttctaagga aaaaggcaac gaagctagaa atggcagcgt
     8461 gccaaggatt ggtaaattct tcatactaaa tcccatattt ttcctctacc agagccttga
     8521 gtggattttt aagaaagatc ttattggtca agagttgggc agaagttttg aattctccat
     8581 tttggatata agcagcatag agggattttt tggctagttc cctatcttgg tctagcaaga
     8641 gtttttctcg cgaaacggca gcttcctcgg tatcttttgg agttgaaacg gatacatatt
     8701 ctccaccagc cattgaaaag gcaccagcta agatagccgc aaaaccggat aaaaagataa
     8761 tccagatatt gctcgtggca ctggcaactc cgataaccac accagcaatg gaaataattc
     8821 catcgttagc atcaagaaca cccgcacgca ggatatttaa acgacctgca aaatttgaat
     8881 caatttcgtg atttgtttct ga
//
LOCUS       DAVDWM010000067         8481 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVDWM010000067 DAVDWM010000000
VERSION     DAVDWM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.067"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..283
                     /locus_tag="VUW49_001976"
     CDS             <1..283
                     /locus_tag="VUW49_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7981840.1"
                     /translation="EFDRTVFESIVEKIIVGGVNIDGEIDPAMLTIIFKTGETQNKDG
                     KKFKSKRKNAKLEPDKLCPQNSDEDKKLYSQGTDNTRRDGSTFVQTRCR"
     gene            231..551
                     /locus_tag="VUW49_001977"
                     /pseudo
     CDS             231..551
                     /locus_tag="VUW49_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
     gene            765..998
                     /locus_tag="VUW49_001978"
     CDS             765..998
                     /locus_tag="VUW49_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HET7981841.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCN"
     gene            1289..1507
                     /locus_tag="VUW49_001979"
     CDS             1289..1507
                     /locus_tag="VUW49_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981842.1"
                     /translation="MALNYKQLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            1900..2925
                     /locus_tag="VUW49_001980"
     CDS             1900..2925
                     /locus_tag="VUW49_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7981843.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKAHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            2915..3922
                     /locus_tag="VUW49_001981"
     CDS             2915..3922
                     /locus_tag="VUW49_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7981844.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            3922..4929
                     /locus_tag="VUW49_001982"
     CDS             3922..4929
                     /locus_tag="VUW49_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7981845.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            4929..5723
                     /locus_tag="VUW49_001983"
     CDS             4929..5723
                     /locus_tag="VUW49_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981846.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVMANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            6111..6536
                     /locus_tag="VUW49_001984"
     CDS             6111..6536
                     /locus_tag="VUW49_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7981847.1"
                     /translation="MPKRYLLDGKTPRISVTNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYIEDLKLKCNIANYNI"
     gene            6592..6909
                     /locus_tag="VUW49_001985"
     CDS             6592..6909
                     /locus_tag="VUW49_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HET7981848.1"
                     /translation="MMASKRLSIEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNK
                     RLIEKGAVFESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKD
                     EIT"
     gene            7223..7897
                     /locus_tag="VUW49_001986"
     CDS             7223..7897
                     /locus_tag="VUW49_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HET7981849.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEKWRENFSDLCNKYLERAGAEKRVD
                     HRSFKR"
     gene            7946..>8481
                     /locus_tag="VUW49_001987"
     CDS             7946..>8481
                     /locus_tag="VUW49_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickase"
                     /protein_id="HET7981850.1"
                     /translation="MERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLKGWIGNLLDN
                     LSTAYEKFKDIERDKVIDNPKLFNLTNYLLTYSEIQKEKSKYLKGYAKTNKEKYDFKK
                     LISAYSYLRKNNIETIGQLQTKIETLKSNSYRLNKKAKTIHKEMEDVEKKILYYEIYK
                     AKKEVYEEYQKKNIFTKEA"
BASE COUNT         3424 a         1113 c         1435 g         2509 t
ORIGIN      
        1 ggagtttgat cgtacagttt ttgaaagtat tgttgagaaa attattgtag gtggtgttaa
       61 tatcgacgga gaaattgatc ctgcaatgtt aaccataata ttcaaaacag gtgaaactca
      121 aaacaaggat ggtaaaaaat ttaaaagtaa acgtaaaaat gctaaactag aaccagataa
      181 attgtgtcct caaaacagtg acgaggataa aaaattgtat tctcaaggga cagacaacac
      241 acgtcgagac ggtagcactt ttgtccaaac tcgatgtcga taagcacata agtgttgaaa
      301 ttgagctgga tgagatgaat ttgacaagtg cggagagcaa agcaacatat gctcaaatca
      361 aagaatatgt ttggaataaa tttgaattaa aagtttcgac attatatatt gcacagataa
      421 aaaagaaatg tggaatagaa ttacgagaac attacaacaa gtctaaaaag gataaacaaa
      481 ttattccaca gtgtacacct gaaaaagaag aagccatcat ggatgctttg agacacttca
      541 aaatgattta atagaaaaga atgacagtat atgactttct gcatttatta cattcctact
      601 tggtatagga acagctatta ttcctttctt gcaaggtatc aattagaaaa taggctcaat
      661 ataaagattg ataggatcat ttttatattt aaaggagcgt tgaaatgatt gataaaggca
      721 acaaaaaatt ttaggataaa tttgctaagt tgtatgcccc ttttatgaaa aaagataaag
      781 aggtttatga taaagtttgt gaatatctta gtcctcattt gaataaagat atggaggtgc
      841 ttgaacttgc ttgttggttt cgtgtcataa tagttataga ggcaaatagt tatgtaaata
      901 taaggagttc aagacttcta ccaaagttta aaactcaaaa aataaatagt tggtgtgctg
      961 cttatagtat ccattttaat aatggatatt gtaattagca taccaatgaa tttaaagatt
     1021 ctttaaagag tcttattttg tgatgaaaat ttaatatgta aatctcagac gatagaaatt
     1081 aaaaactcta tcgtcttttt tatactcaaa attaggaggt aaaaatggta aggataagag
     1141 gtcccactta aaacaattta tggcaaaata aggacggaat aacacaacaa attctctaaa
     1201 acaaatcact aaatcaatgt aagattgaat gaaatcaata tttatgctat aattaaataa
     1261 atctaatgaa gaaaaaaaga gggatattat ggcacttaac tataaacaat tatggataca
     1321 gttagcaaaa aaaggactaa agaaaacaga tgtaatagct atggcaggac ttacaacaaa
     1381 tgttatggca caaatgggaa aggataaacc aattacattt aagaatttag aaagaatatg
     1441 taaggcttta tcttgcactc ctaatgatat tattagtttt gaagataatt ttagtgacga
     1501 ggaatagaaa atgactttaa ggacagaaga tcaagttagg gattatgcaa gagaagtata
     1561 ggctttaatg aagttgaaga aaacatcaat caaggtactg gtcaaataac tacttttaat
     1621 caattaggct tcaagggata ttcagataag ccagatggtt ggtatttacc taaaaatatg
     1681 aatgatgtag caataatcct tgaaacaaaa tcagaagaaa gagatattag caaacaaatt
     1741 tttattgatg agttaatgaa aaatatagac ataatttaac taaaaataaa aactagatcc
     1801 ttttttgaaa aaattatatt attaaatttg taactgtatc tattgacaat gataattatt
     1861 atcgatacaa tagacttgaa atatgtttaa ggagttttta tgaaaaacaa attttttcta
     1921 atagctattt tagctatgtg tatagttttt agcgcttgtt cttctaattc tgttaaaaat
     1981 gaagaaaata cttctaaagc gcatgcgcct gataaaatag ttttagatca tgctttcggt
     2041 caaactatat tagataaaaa acctgaaaga gttgcaacta ttgcttgggg aaatcatgat
     2101 gtagcattag ctttaggaat agttcctgtt ggattttcaa aagcaaatta cggtgtaagt
     2161 gctgataaag gagttttacc atggacagaa gaaaaaatca aagaactaaa tggtaaagct
     2221 aacctatttg acgatttgga tggacttaac tttgaagcaa tatcaaattc taaaccagat
     2281 gttatcttag caggttattc tggtataact aaagaagatt atgacactct atcaaaaatt
     2341 gctcctgtag cagcatacaa atctaaacct tggcaaactt tatggagaga tatgattaaa
     2401 attgattcaa aagccttagg tatggaaaaa gaaggtgatg agttaatcaa aaatactgaa
     2461 gctcgtatat ccaaagaatt agaaaaacat ccagaaatca aaggaaaaat caaaggaaaa
     2521 aaagtattat ttactatgat taatgctgca gatacatcaa aattctggat ttatactagc
     2581 aaagatccaa gagcaaatta tttaacagat ttaggtctag ttttccctga atcattaaaa
     2641 gaatttgaga gtgaagatag ttttgcaaag gaaatttctg cagaagaagc aaataagata
     2701 aatgatgctg atgtaatcat aacttatggt gatgataaaa ctcttgaagc tttacaaaaa
     2761 gatcctcttt taggtaaaat aaatgcaatt aaaaatggtg ccgttgctgt aattccagat
     2821 aatacaccgt tagcagcctc atgcactcca acaccacttt caataaacta tactattgaa
     2881 gaatatctaa atcttttagg aaatgcatgc aaaaatgcga aataaaaaac aaataaacct
     2941 aggcataatt tttgtaatct gcctaggtct tcttattaca atatttttgt cattaaagct
     3001 tggaacaaaa gaaattaata tcagagattt tttagcagct tttggaatgg gtaatacaaa
     3061 tgatgatttt attaaatcaa ttatatataa aagaatacct agaactattt ttgcaatttt
     3121 agcaggttct agtcttgcca taagcggtgt attgatgcaa tcagttacta gaaacccaat
     3181 agctgatcca ggtatactcg gtataaacac aggagcaagt cttagtgtag taattggtct
     3241 ttctttttta ggaatttcat caagcataag ccatataagt tttgcaatca ttggtggctt
     3301 agtaagtgca atttttgtat acgcgattgc tgtaagcgga aaagcaggcc ttacccctat
     3361 aaaacttgcc ttatcaggaa cttgtgttag tatggcttta agcagttttg taagtttttt
     3421 aattttaccg aataataacg tcttagacaa atttagattt tggcaaatag gtagccttgg
     3481 agcagctaca ttatcttcta tatctacact actacctttt ataattttag gtcacttgat
     3541 agctatattt atttcatcag atttaaacgc tttagctatg ggtgatgaaa tggctgttgg
     3601 tcttggagtt aatgttaata ggataagatc acttgcaata attgcaagtg tgcttttatg
     3661 ttcaagtatt actgcaattg gtggacctat tggcttcgta ggtcttatag ttcctcactt
     3721 ttgtggctta tttataagca aagatatacg cacaatgacc atttcttcat cttttatagg
     3781 tgcagagctc ttgcttatat gtgatataat cggccgtatg ttaggtaaac caggtgaaat
     3841 tgaagtaggg ataattactg caataatcgg gggtccagta cttatttatg taactatgaa
     3901 aaatagaggg gttaataact aatgcaaaat ttaattatag gtattcaaaa aagaaaaaat
     3961 agaataacac tattctcctc actattttta ttaataataa tcagtctatc atttttcatt
     4021 ttacttatcg gagatgaaag ttattctttt tcaactttga ttaaagtctt aaatagtgaa
     4081 actgttcctg gagctagttt ttcgattatg gaaattagat taccaaaatt attagcagga
     4141 attatagctg gctggtcttt tggattggca ggatttatct ttcaaactat gttaagaaat
     4201 cctcttgcaa gtcctgatat aatcggtgtc acaagttctt catctattgc agcggtcttt
     4261 tgcatattgg tattaaaaac aaatagttta actactggaa ttatttcaat aacttgtgga
     4321 ctaacatcat ctttaatatt atttttacta gctaaaaaag atggtttttc agcagcaaga
     4381 ctgataatat taggtattgg ttttcaagct gtcacaagag caggcacctc atttttattg
     4441 ttgaaagtag caagatatga attacaagaa gttatgagat ggctcagtgg ctctttatct
     4501 tttacaaagt tagatgacat acctcttgtt ctaatagtaa gtattattgc tactatatta
     4561 gttttatttt ttaataaaag actagaaatt attgaacttg gtgaagaaat agcaatcgga
     4621 cttggagcaa atcccgagct ttcaaggctt gttttaattt tttgcgctgt atctttaact
     4681 gctttttcta cttcaattac aggaccaata gcttgtatat cttttttagc tggtcccata
     4741 gccttaaata ttggcaagaa aagaagccca atattagctg gattggttgg aattttacta
     4801 gttttgttat cagacatatt ctctcaaaat attttaccag ctagatatcc agtaggtgtt
     4861 gtaactggct tgttaggttc accatactta atatacttac taataaaaat gaacaggaga
     4921 aatatataat gaaaggtctg tggtcaaata atcttacctg cggttatgat gaaaaaataa
     4981 tcttggaaaa tataaatata aaaatacctg aagaaaaaat atcagttatt attgggtcaa
     5041 atggttgtgg gaaatcaaca ctcattaaaa ccttgtctcg acttataaag ccattagagg
     5101 gagaagtatt gcttgataat aaatcaatta attcttataa agaaaaagat ttagcaaaac
     5161 acatagctat attacctcaa tctccaataa tccctgaatc aataacagta gctgatcttg
     5221 taagccgtgg tcgtttcccc tacagaaagc cttttaagag tcttggaaaa gatgaccttg
     5281 aaataataaa cagatcaatg gttatggcca atgttgaaga tctagcaaat aacctagttg
     5341 aagaactttc tgggggtcaa aggcaaagag tatggatagc tctagcccta gcccaagata
     5401 caagtatcct acttttagat gagccaacta cttacttgga tatctcatat caaatagaac
     5461 tattagacct cttgactgat ctaaaccaaa aatataagac aaccatttgc atgattttgc
     5521 acgatataaa tctaacagca agatacgctg attacctatt tgcaattaaa gaaggtaaac
     5581 ttgttgcaga gggaaagcct gaagatatac taaatgataa actagttaaa gatatcttta
     5641 atcttgaagc aaaaattata cgtgacccta tttccaattc gcctctaatg attcctattg
     5701 gcaagcacca tgttagctct taataattga atattggaat tcatttaaaa gtcgcaaatt
     5761 ttgaactgac cctcaaaagt taggccaaaa aactaactaa aggaggtcag ttttttaatg
     5821 gcaaatacag cacagaattt aagataaaat tagtaaaaga atatcatgaa tgaaaaatag
     5881 gatatagaga ataacagcaa tattaaaaag atcaggactg ataataaaca aaaagaaagt
     5941 acaacgacta gttcaaaagc taaaacttca agtaaaaagt tattcaagaa aatctagaaa
     6001 atactcatcc tacaaaggac aggtaggaaa aatatcagac aacaaaataa aaagaaatat
     6061 ttattaccga aaaaaagggg aaatcgagtc aagtaccaac aggctcatct attccaaaaa
     6121 gatatttact cgatggaaaa actcctagaa ttagtgttac aaatattgac aatggtatct
     6181 taggttacta cgaagatata gatgataaaa attatagagt ttttgaaaat tttatatctg
     6241 tttcattttt aggagcagtc ttttatcata aatataaagc aagcctagat atgaaaattc
     6301 attgtttaaa attaaaaaat aaagaattga ataaagaagt agcattctat ttaacttcaa
     6361 ttattaggca agctttaaaa aatactgaat ataaagatca aatatctagc acagtattgc
     6421 cagatatcaa aattaaatta ccgatagaca gtagaggtac tccagattgg aattacatgg
     6481 aaagatatat agaagactta aaattaaaat gcaatatagc aaattataat atttaaaaca
     6541 agaacgatta gaaataaaaa tctagtcgtt ttttatttta agaaaggata catgatggca
     6601 agtaaaagat taagtattga agaacagatt gaaaagaaag aagaaagcat taagcaatta
     6661 caaaatcaaa aaagacagtt gaagaaaaaa cttaatgaac aagaaagaaa agcaagaaac
     6721 aaaagactta tagaaaaagg tgcagtgttt gaaagtattt ttgaagaaag tattgattta
     6781 acaaaagatg aattttataa gttaataaaa acgttaaatg atgaagaaat aagattaaac
     6841 ataatggaaa ttttagaaga aagaatagat gataatgtag aaaaatcatc taaagatgaa
     6901 ataacataat tgtcccttgg ttacaagggc gcaattatac accctaaagg gtgcttgcgt
     6961 cctgcggagt caaacccttg cagggagcaa caaccattcg ctttttaaaa gtcaagggta
     7021 aataaactcg ctaaggctcg cccttgactt ttaaaatttg aaatgaaagt aacgattaag
     7081 ccgacatact gaaacaacaa aaattaattc agtagtcggc ttttttaatt ctatcatttc
     7141 aaaacgctca ttcacaaaga ggatataaaa aaactattaa gcctccacct aaaacaacaa
     7201 agaaagaaag gaagtgataa aaatggcaga cagttttcat ttttcagtaa acataatatc
     7261 aagaggaaaa ggcaaaagtg cagtagcaag tgcagcatat ataagtggag aaaaaataaa
     7321 aaatgaatgg gacggagtaa cccatgacta cacaagaaaa gaaaaaatat tagtaaaaaa
     7381 tataatattg cctgatcata taccaaaaga atttaatgat aggtctacct tatggaataa
     7441 agtagaaatg gcagaaaaaa attctaatgc acaactagca agacaattca taataggact
     7501 accaaaagag ttatctttaa gtgaaaataa aaacctggtt gaaagatata taaaagaaaa
     7561 tttaacatca caaggaatga tagtagatta tgcaattcat gatgagagtc aagataaaaa
     7621 tggaaatatc cattgtcata taatgaccat aatgcgacca ataaacgaaa aaggagagtt
     7681 cttagccaag tcaaaaaaag aatatatcct agatgaaaaa ggagaaaaag tattaaacaa
     7741 aaatggaaaa ccaaagacaa gaaaagtaga actaacaacc tggaacgata caggcaatgt
     7801 agaaaaatgg agagaaaatt tttcagatct ttgtaacaaa tatttagaaa gagcaggagc
     7861 agaaaaaaga gtagaccata ggagttttaa aagataaaat tcagattatc taccgacaat
     7921 ccatttagga tcagcagcaa gtgcaatgga aagaaaggga atagaaactg acaagggaaa
     7981 ctataataga gaaataagaa aatataatca acttgtaaaa acaataaaag aagagataaa
     8041 aacattaaag ggttggatag gaaatttatt agataactta tctactgctt atgaaaaatt
     8101 taaggatata gaaagagata aggtaataga caaccctaaa ctttttaatc taacaaatta
     8161 tctattaact tattcagaaa ttcaaaaaga aaaaagtaaa tatctaaaag gatatgcaaa
     8221 aactaataaa gaaaaatatg acttcaaaaa gctaataagt gcatatagtt atttaagaaa
     8281 aaacaacata gaaaccatag gtcagttaca aacaaaaata gaaactttaa agtctaatag
     8341 ttataggcta aataaaaaag caaagacaat ccataaagaa atggaagatg tagaaaagaa
     8401 aattctatac tatgaaatct acaaagccaa aaaagaagtt tatgaagaat atcaaaagaa
     8461 aaatatattc acaaaagaag c
//
LOCUS       DAVDWM010000068         8133 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVDWM010000068 DAVDWM010000000
VERSION     DAVDWM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.068"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(204..965)
                     /locus_tag="VUW49_001988"
     CDS             complement(204..965)
                     /locus_tag="VUW49_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET7981851.1"
                     /translation="MEIQDYTDSEFKHALARNFRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQENGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFKSIQIYQRDRSCVYDSKENTT
                     SAATVLHDLLFGEWSQIEKEMLQVGEKRLNELLEK"
     gene            complement(965..1441)
                     /locus_tag="VUW49_001989"
     CDS             complement(965..1441)
                     /locus_tag="VUW49_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981852.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(1886..2167)
                     /locus_tag="VUW49_001990"
     CDS             complement(1886..2167)
                     /locus_tag="VUW49_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981853.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            complement(2616..4355)
                     /locus_tag="VUW49_001991"
                     /pseudo
     CDS             complement(2616..4355)
                     /locus_tag="VUW49_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            complement(4651..5316)
                     /locus_tag="VUW49_001992"
     CDS             complement(4651..5316)
                     /locus_tag="VUW49_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981854.1"
                     /translation="MGLYKNQEKLEEKMKDKESYNKFIPKLYNEELLKNHFKETMCFS
                     RKELNNKSIINQKLIKFPIVGHEVWALFGKEKTGEWRCLQVGQSKNKVKAEIESLIEF
                     MSYDYNQLVDSIKDGVKNRNSTFYSNIYQSSKKSKNEFLYSHIASQYDEFQLCLLDID
                     KYLGFKNLKFENKHISNIMKIAKPLYAEAKLAFETKSIYWTMYNSGVDGQAIMIFLGD
                     NKD"
     gene            complement(5421..6302)
                     /locus_tag="VUW49_001993"
     CDS             complement(5421..6302)
                     /locus_tag="VUW49_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET7981855.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKE"
     gene            complement(6414..7253)
                     /locus_tag="VUW49_001994"
     CDS             complement(6414..7253)
                     /locus_tag="VUW49_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET7981856.1"
                     /translation="MLGAIIGDIVDSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGSMYPNADYGARFNAWLNSDNSFGNGSAMRISPCAWIMD
                     CGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCREYEQS
                     INDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFLESRDF
                     EDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRRWENRI
                     KAY"
     gene            complement(7311..8069)
                     /locus_tag="VUW49_001995"
     CDS             complement(7311..8069)
                     /locus_tag="VUW49_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET7981857.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
BASE COUNT         2513 a         1620 c         1150 g         2850 t
ORIGIN      
        1 agttcatctt cagtaatttt tctatatttc gttgcagtcc aattagaaaa tagcttgtaa
       61 tcataagaaa caggtgtatg caaatgaatt aaagtcttgt aaaagctacc gaatttctct
      121 ttggaatttg ttagttcttg ataaacagac tttatcttgt tattaaaccc tctcatttct
      181 actttcctaa aaatatcaat tgtttatttt tcaagtaatt cattaagtct cttttccccc
      241 acctgcaaca tctccttctc tatttgactc cactccccaa atagtaaatc atgaagaacg
      301 gttgctgctg aagttgtatt ttcttttgaa tcatatacac aacttctatc tcgttggtaa
      361 atttgaatgc ttttaaagat agccagttct tccaattgtc gtgtattatc aactagatga
      421 tttacaatga aatcatgatg ttcttttgga gttgcgcgtg cttgatttgg attgatagca
      481 tacagttctt cgtatcggat aagggtgctc agataggaca attcgggctt ggttgcaatt
      541 aaggctaatt gtacctcata ccccttattt tttaatagtt gtgctgtttt ctttggaaca
      601 tcaatagttc gtaaagttcc ctctatcaaa agattgtatc ccaaatgact caattcggtt
      661 actaaagact ctaccatttt ccctgcaaaa tctttggtgt attcaacgct atctttgcca
      721 ttttcttgct gcagttctaa atagtgtgga tgctgagaac gaaaactatc accatctatg
      781 ataacaatat ttccttgaaa ttctttctgt ttaatacgat gaattgtagt cttaccggca
      841 ccactttgac ctccaagcaa aatcgctata ggttgtttac tggacttttt tcctcttgtc
      901 agtgaacgaa aattccttgc taaagcatgt ttgaattcac tatcagtata atcttggatt
      961 tccattaagc taccatccgc ttttcagata tctctaacat acgttcaatt ccatccaaat
     1021 agccgctata tctctctatt tcatcaaaat tttctactaa atagatattt gtctgaatca
     1081 agtcagatag atcatcactc attaaaatcc aaggattaga ttcatcatca atgctaattc
     1141 cctgactatc ttgataacga tagagtcgag ataatagatt agcacctctt tctttcacaa
     1201 tttcaatttt caaagtcaat tcataatctt caacaggatt gagcatttta tcttctccta
     1261 caatatcgac ataagataca ttaaacttct gacaaatgat gtcaattaat tccgtagaga
     1321 ctgaactagt tccattttca taacgactca agctatttcg agaaattcct ataattcgtg
     1381 caaattcgtg ttgtgttaag tcatgtgttt tacgtaagga ttttatgttt tttccaatca
     1441 tggcaaactc ctttattttt ataaatccat tatagcataa aaaagcataa cgcaccaaaa
     1501 atggtgcgtt atttcttgtt tttctatcct aaactttact tctataaaag tcacctaaag
     1561 agccataaat atttattctt gtattaattt tttcgatgcg caaacgatat ctacaaatac
     1621 atctataacg attcaccttt taggattttt attgtcaaaa ttgcctttat ctacaaagac
     1681 aaaatgctta taaaatagct tgaaatcaag gattatcacc tttttcgttg tagcaacact
     1741 ccacatgtga ttattaggct attagcaaag cgtatccaat acctcaataa agtgttgttt
     1801 ttactgatat tttaactata ttgtatagtc tactatttaa atctcaatta ggatatttta
     1861 tagaataccc aaaattcaac aataactaaa agtaagattc cattgtattt ccttgttggt
     1921 ctttcatatc acgaatttta tttttcagaa tattaaagtt acaaatttca tctctaatat
     1981 cgttatccgc tctgtagcca ttaatcatct cagtcagagt tttcccttta ctaccattct
     2041 ttatagtagt atttactctt tttatactgc tttcaaaagg ctcatatgtt ttatacttaa
     2101 ttacttcgtc tttaaaatta aataaaggtg tatccggctt agcattccat aaaataatca
     2161 accacattat agcattataa agctgctttg cagtttcatt ccacttttca ggttataaat
     2221 ttccactccc tgtattgtca tattcttgtc tattatttac atgagaatca cctacaagcg
     2281 gattaaaaag aggtagccct ttgtgattaa ttagttctaa aaaatccctt gccgcaccca
     2341 tgcaacactg aataacttcc ttcttgccat caattttact ctcagcgata aatacataat
     2401 attcatcttc aatagttgct cctgcatcac tatgtttttg ttgatttgct aacagcttaa
     2461 tatgtgcttt aggctgaact tcaaaatctc taacaattct ttgtcttgtt tcatgtcctc
     2521 tacaatttat ttaagttctc ctttacaagt ttagtaacta gccttattat actatataag
     2581 gtatgaaatg taccataatc ttcatcaact agtttttaga aaatcataaa tccatctgga
     2641 gagatgacaa gctgattatc caattcttgg caattgcttg ctagtgctac tgcttctttc
     2701 tctaaaagat aatcttctgt tgattggttg aatattgctt tgaggatccg tccttcgtat
     2761 ttccattcca gagctactag atcagagttg atttcttgaa ggctatactt gccatgcgaa
     2821 atgattactg acgcgtattt ccgaatttta atcagcctct tcataaagtt cagcatatca
     2881 ttgtcacttg atacacgttc ccaaggcata caacgacgac aatctggatc tggtcctcca
     2941 gtcaaggcta gctcggttcc gtaataaatg cacggtgttc ctttttgtaa aaagagaaag
     3001 gctaaggctg atttaaccag ttgaacatct tcattggccg tccacaggat tcgctctgta
     3061 tcatgtgaat ccaagagatt aaacatgacc tctgaaatct gctgcttgta atacatagac
     3121 tctccattga tttcatcgat gaactggtct gtcttcttaa ttcctcgtaa gaaatagtcc
     3181 ttgatactat cagataaagg ataattcatg acggcatgga actcatctcc atttagccaa
     3241 ggctgagatg tatgccagac ttctcctagg atataaagat caggattttt agctaaaact
     3301 gccttacgaa aatccttcca gaactgatgg tcaatctcat tagccacatc caaacgccaa
     3361 gcatcgatat taaactcttc aatccaataa gtcgcaacct ttaaaagata attcttgacc
     3421 tctggattgg ctgtatttag cttaggcata tagtcctcga aaccaaaaac atgatagggt
     3481 aagtctctct tattaactag cttttcagtt gtcactggga attgttgaat atggaaccaa
     3541 tccttataag cagactgttc accatttttg acgacatttt ttcattgaag agattgcgaa
     3601 ccaatatgat taaataccgc atccagcatg actttcatgc cacgatgatg cgcttgatcc
     3661 accagttccc gaaaggtctc cttgtctcca aaatgacggt caatttcaaa gtaatctgtc
     3721 gtattgtact tgtgattgct tgtagattca aagatgggac aaagatatag tccagtaata
     3781 cccaagtctt gcaagtaatc catatgatca ataatcccct gtaaatcacc accaaagaaa
     3841 tcatcgctct taggtgtgac agatgaatcc cagtctaaag tcccttctgg gtttaataga
     3901 gcattgccat tggcaaatct ttcaggaaat atctgatacc ataccgtatt tgaaacccag
     3961 tcaggaacct tgcaggcatc aatctcatga agataaggca acttaaatcc attcccaatt
     4021 gcatgaagat tttctagaga attttccaca caccctttat cgccatacaa aatattttga
     4081 ccttctgtat ctctgagctc aaagagatac tggatacgtg caaagtcaac tgacacttca
     4141 acctgccaat ggtcaaataa ggtaccagaa gttatcttga ccatttcttt tgtatcctga
     4201 taaaactcct ccataaagat aaaagggtcc ccatagtgca agttgatgct ttcaatgtcc
     4261 cctttcttag ttcgaattcg aatatggagt ttcttatcct tataaagata ggcatactcc
     4321 gactcaggcc tatggtaaat agcactcaat tccatcaatt tgtctcctaa agtattcatt
     4381 caaattttac gcaaacgttt tcaaactcaa tgaatccagt atactatttt tgtaaactat
     4441 ttgcaaggaa tgtccgttgt taaaggaact atcttttaaa gtatacaaaa agccttaaaa
     4501 aaggctttga attactacta ttgattagaa ggatataaag tttacaagta tggtacaatc
     4561 tattcaaatt taattagtaa ttttgaattc caatttcata tagactcaag atagtagtat
     4621 atatttgaag atagaaaaga agacatttaa ttaatctttg ttatctccta aaaaaatcat
     4681 gattgcttgt ccatcaactc cagaattata catagtccaa tatatagatt ttgtttcaaa
     4741 tgcaagttta gcctctgcat acaatggttt tgcaattttc attatattac taatatgttt
     4801 attttcaaat tttaagtttt taaagccaag atatttatca atatctagta gacaaagttg
     4861 aaattcatcg tattggcttg ctatatgact gtataaaaat tcattcttgc tttttttaga
     4921 agattgataa atattactat aaaaagtact atttctattt tttacaccat ctttaatact
     4981 atcaactaat tgattataat cataagacat aaattcaatt aatgattcaa tttcggcctt
     5041 tactttattt ttactttgtc caacttgtaa acaccgccat tcgcccgttt tttctttacc
     5101 aaataatgcc caaacttcat gaccaactat ggggaattta atgagctttt gatttattat
     5161 actcttatta tttaattcct tcctagaaaa acacatagtt tctttaaaat gattctttaa
     5221 aagttcttca ttgtataact ttggaataaa tttattataa ctttctttgt ctttcatttt
     5281 ttcttctaac ttctcctgat ttttatataa acccattatc caattatttt aaacgacatg
     5341 ttaattttac aaaatttagc aaatcttaca agtaactact ttcgttgata taaaacttta
     5401 tttctcagtg aatgacaaac ttactctttc ggttttgaaa atacttctac tcgctgagct
     5461 aggactttga tttctacagg taggttatct gatttatctc cgtcaacatc tgactccaat
     5521 tcactatctg aagagatttt aatattacgc gctttgatat actcgatatt atcatttgcg
     5581 acaacatccc cttttaataa atcaggaatg acggataatt tggagaatat agaggcatct
     5641 ttcagaatca aaatgttggc atagccgtcc ttgttttctt caaagatttt cttatcagcg
     5701 aagtaatttg tcaaaaggac caaaacatgg ctagcttcac caacataatt tccattttct
     5761 gtctcaacct taatgttaaa gacctgatct gtcatgacag acttcatggt atttacagca
     5821 taggttaaaa taccgaattt tgttttgtcc tctatttcaa cattgtgaat cgcctcaggc
     5881 agagaaccga tactaaagat ataaccaaaa tagttgtcat ttgctttacc gatatcaatc
     5941 ttattggtta aatcaaaatc cagttcgtca attgcgccat cgatgtcttg attgatttcc
     6001 aaaagttttg taatgaggtt acccgtaccg cctgggataa tccctaactt aggaatgtag
     6061 tctctctcat caatacctga aatgacttca ttgacagttc catctccacc aaacacaacc
     6121 actgcatcat actgctcacg agaagcttct tcagcaaaat gtgttgcatc cagcgctttt
     6181 tcggtaattt tggtttcaac atattcaaag tattcttttg ctttattctc cagcttttct
     6241 ttgtaatcca aagccttctc gccaccagaa gtagggttga taattaccat tgcttttttc
     6301 attgatttta tccttaattt taaacagaaa tgtttacatt tcgtcgtatg caagtaaatg
     6361 taatcctatt atacaatgaa aatacagaaa agagaaatct gacgtactgg agattaatac
     6421 gcttttattc tattttccca tcgcctaact acatccttta agggttcatc caagtaagaa
     6481 taggccttat ccttgatcca atcaggaata ccgtaagctg cctctgctat gctacaagtg
     6541 attgctgcga gagtatcact gtcgccacca agtgagatgg catttcttat cgcatcttcg
     6601 aagtctctac tttcaagaaa ggcgataatg gcttgaggga cagtttcctg acatgtttcg
     6661 ttaaaacgat agttaggacg gatttcatct aaagtttgag atagattgta atcgtattct
     6721 ttttctatat aatccttaat tcgtctttta cactctgtag gattgtcgtt aattgattgc
     6781 tcgtactctc tacaataacc accaaagtaa aaacgacata gaaagatagc atcagctgta
     6841 gccatagcgc ctttgacacc ttctggatga ttatgagtta cctctgcaga aagactcgta
     6901 agtcctctag atgatggcca cataccagtt ttcgcataaa aaccacagtc catgatccaa
     6961 gcacatggag aaatacgcat agctgatcca ttcccaaagc tattatcgct gtttagccat
     7021 gcattaaacc gagcaccgta atcagcattc ggatacatgc tgccatattt cttcatcgcg
     7081 tcaatgaaat cgtctttttg tccaccattc ataattgctt ctgcaacagc acaggtcata
     7141 accgtgtcat ctgtaaaaaa gcagtccttc cgaaataaag gaaagtcctt tgttttgata
     7201 ttgttccatt cgtaaacaga atcgacaata tctccaataa ttgctccaag catcagattc
     7261 ctccttgttc attttgatgc tttttatatt ggttatctac catatttatt ttagaaaata
     7321 acatcctgtt ggattttaaa aatttcattt ttttcaaaat agggttttac catttctttc
     7381 cacctagctc tatgaaaatt gattgatttt aaaggagata ggccataatt tcccaatgca
     7441 taaccatcat ttacttcaac aacaagtgtt ctgccatcgc gagtaacacc gatatctagt
     7501 ccataagcta ttggcgcatc tttccaacat gatatcgctt catcaattac acttgcatca
     7561 aattgtgcat gataatcacc tgtatagggt cgaacatcta atacgcgacc atctaacaca
     7621 aaacaacgcc attcagctat gaattctaca acctcactaa tccatatagg atagtcgaaa
     7681 ggtagaccaa tacctattaa atcatgggtt ccattaacaa cccttccagt aaagactttt
     7741 gaaccagctt taggcttaat aaattttccc caattatcag gtatattcac aatctctcct
     7801 aaaataccag cataaatctt tcgaccataa aactctttaa gctcaatagg atagtcataa
     7861 accggaacgt ttaagcccat catttttagt aatgctctag tctccattat ataatctaca
     7921 actatatctt cacttgttaa ctcttttatt tcagaaaaag attgatataa aataacttct
     7981 tctccttgta agtaggcacc tacttgagca ttgtatttat taattgaaac ctcacttggt
     8041 aatttacttt gtctaatata aacaaccatt tcatcactcc tatatcacta gtgttacacc
     8101 aatttgtaaa aaataatagt aattttgctc tta
//
LOCUS       DAVDWM010000069         7801 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVDWM010000069 DAVDWM010000000
VERSION     DAVDWM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.069"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            352..972
                     /locus_tag="VUW49_001996"
     CDS             352..972
                     /locus_tag="VUW49_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981858.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1058..1501
                     /gene="tsaE"
                     /locus_tag="VUW49_001997"
     CDS             1058..1501
                     /gene="tsaE"
                     /locus_tag="VUW49_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7981859.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVVILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            1491..2009
                     /locus_tag="VUW49_001998"
     CDS             1491..2009
                     /locus_tag="VUW49_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981860.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEATEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2017..3033
                     /locus_tag="VUW49_001999"
     CDS             2017..3033
                     /locus_tag="VUW49_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7981861.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYKDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3118..4374
                     /locus_tag="VUW49_002000"
     CDS             3118..4374
                     /locus_tag="VUW49_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET7981862.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            4429..5595
                     /gene="recA"
                     /locus_tag="VUW49_002001"
     CDS             4429..5595
                     /gene="recA"
                     /locus_tag="VUW49_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7981863.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(6167..6316)
                     /locus_tag="VUW49_002002"
                     /pseudo
     CDS             complement(6167..6316)
                     /locus_tag="VUW49_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6381..>7801
                     /locus_tag="VUW49_002003"
     CDS             6381..>7801
                     /locus_tag="VUW49_002003"
                     /inference="COORDINATES: protein motif:HMM:NF018196.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680869.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET7981864.1"
                     /translation="MKIKSNRQTDFRTEKYTCFGIRKYSFGVASVAIATGLMFLGSGT
                     VSANEPTSKETGDQVVVENNKSDLLKENQRTESRYESPAVIPVTNSKESQSSENNKEI
                     QSLPSESTTNSVLKDAVENEVEKVEKQPNKSNVEDRSVSTDTDVSLRRTRRDLDSNIV
                     EKAGSISITSPKENTSSDSSHPNGFSPVTKYHDASDGWYTFVQLDQQRQNQNIRVVDS
                     YDGYVVVSAKSEQTSAGFTTDGTLKLEFYNRNNELQQEIIMDKNTNSLEFVFNRNKKL
                     IINRTANSISIGDMPYTRKTRNQIANFNILYNGPEISNKQGQGPFYADGWLIPTNNSV
                     TVRFKDKDTNRELVPSHTLNGLTGMRFDTTAAELKGYELVEKEIVGSPNGYISPWKKV
                     GDSFTKVTQPNLEVKYTLLDMDGTMKTEVYYSGKKIHEITLKKGETSYYNGTAANRRP
                     YTQVLENPFLVATNEVVYYYKATT"
BASE COUNT         2513 a         1230 c         1727 g         2331 t
ORIGIN      
        1 ataacctgta aggctgatag ttagattaat actatcagcc ttataattat gtgactctga
       61 gtccgtctcg ctccgtgagg tgcgagacaa aacaaccacc cgtttgacgg attttcatga
      121 tttcaaattt atattcttcg aaaatctctt caaaccacgt cagctctatc tgcaatctca
      181 aaacagtgtt ttgagcaacc tgtggctagc tacctagttt gtttgctctt tgattttcat
      241 tgagtattag tctctagtgt ttgtttagcg tcttaaagag acatttgaga agattagaaa
      301 cagttctctc ttttctccta gtataatgat tggtaggaag ggagagagaa gatgaaatca
      361 atgagaatct tatttttgtt agctttaatt caaatcagtt tgagtagctg tttcctatgg
      421 aaggaatgca tcttgtcctt taaacaaagt acagcttttt tcatcggaag catggttttc
      481 gtttcaggaa tctgtgctgg agtaaattat ctttataccc gtaagcaaga agtccatagt
      541 gtcctagcca gtaagaagtc ggtgaagctt ttttacagta tgttactctt aattaatttg
      601 ttaggagctg ttcttgtttt gtcagataac ttgttcatca aaaatacgct gcagcaagaa
      661 ttagttgact ttttattgcc atccttcttt ttcctatttg ggctagattt gctgattttt
      721 ttacccttga aaaaatacgt gcgcgatttt cttgctatgc tagacagaaa aaagacagtg
      781 ttggtgacta ttttagcaac acttcttttc ttaagaaatc caatgaccat tgtctcactt
      841 ctgatttata ttggactggg cttgtttttt gcagcctatc ttgtcccaaa ttcggttaag
      901 aaggaagttt ccttttatgg tcatattttc cgagatcttg tattggtcat tgttacgctc
      961 attttctttt agagaaagcc tgttttttat ggatgttatg atgataagtt tagtaggaaa
     1021 atagacacaa aagaaaagtt tttggtataa taagagtatg tacacaaaaa atgaagaaga
     1081 gttgcaagcc ttaggggagc gtttgggcca tctattagca aagaatgatg ttgtaatctt
     1141 aactggagaa ctgggtgcag gtaaaacgac ctttactaaa ggacttgcaa aaggattaca
     1201 gatttctcaa atgattaaaa gtcccaccta tactatcgtg agagagtatg aaggtcgact
     1261 tccactttat cacctagatg tttatcgtat tgaaggagat gctgattcta tcgacttgga
     1321 tgagtttatc tttggtggcg gcgtgactgt tattgagtgg ggaaatctct taggagatgc
     1381 cttgccagat gcttatttag aattggaaat tctaaaagaa gcagatggac gccgtttaaa
     1441 ttttcaggca aagggtttgc gtgctgagaa attgttagag gagcttcaat atggagtatg
     1501 aattgctcat tagggaagca gagcccaaag atgcagctga attagtggcc tttttaaatc
     1561 gtgtgagttt ggagacagac tttaccagcc tagacggaga tggtattctc ttgactagtg
     1621 aggagatgga aatattcctc aacaagcaag ctagttcgga caatcagata accttacttg
     1681 cattcttaaa tggcaaaatt gctggtattg taaatattac agctgatcag cgtaagagag
     1741 tccgtcatat tggagatctc ttcattgtga ttggaaaaag atattggaat aatggcttgg
     1801 gaagtttgtt gctagaagaa gcgacagagt gggcacaagc aagtggcatt ctgcgtcgtc
     1861 tccaactgac tgtccaaact cgtaatcaag cagcagtcca tctttatcaa aagcatggct
     1921 ttgtcattga aggtagccaa gagcgtgggg catatataga agaagggaaa tttatcgatg
     1981 tttacttgat gggtaaactg atagattagt agaaatatgg ttaaaaaaat tattggaatg
     2041 gtgctagctt tactttctgt aactgtagta ggagtaggtg tttttgctta tactatttat
     2101 caacaaggga cagaaacctt agctaaaacc tataaaaaaa tcggcgaaga aaccaaggtt
     2161 attgaagcga ctgaacctct aaccattctg ttaatgggag tggacaccgg aaatgctgaa
     2221 cgaactgaaa cttgggtcgg tagaagcgat agcatgatct tgatgacagt gaatcctaaa
     2281 acgaaaaaaa caacaatgat gagtttagag cgggatattc tgacgcgcat tgaatcaggg
     2341 aatggtcagg ctcatgaagc gaaactgaac tcagcatatg cagatggtgg agcagagctt
     2401 gctatagaaa ccattcaaaa aatgatgaat atccatattg atcgctatgt gatggtcaat
     2461 atgagagggt tgcaaaaact agtggatgca gtaggaggta ttacagtcaa taatatccta
     2521 ggtttcccaa tttctatcag tgaccaagaa gaatttaata ctatttctat cggtgttggg
     2581 gagcaacata ttgggggaga agaagcccta gtctatgcac gaatgcgtta caaagatcct
     2641 gagggggatt atggtcgtca aaaacgtcaa cgtgaagtta ttcaaaaagt catggaaaaa
     2701 gctctcagtt taaatagcgt tggtcattat caagagattc taaaagcttt gagtgacaat
     2761 atgcagacca atattgattt gtctgcaaaa agtatcccta acttgctagg ctataaagat
     2821 tcatttaaaa ccattgaaac tcagcagttg cagggtgaag gagagatact tcaaggtgtt
     2881 tcttaccaga ttgtttcgag agcacatatg ttggaaatgc aaaatatact ccgacgttct
     2941 ttgggacaag aagaagttac tcagcttgaa accaatgcgg ttttatttga agatttattt
     3001 ggcagagcac ctgttggtga tgaagataat taacttttga tacaaataaa aaaatcaatc
     3061 gtaggaaaat tcctgcgatt ttttcaaaaa aatacgaata gataggtagg aggaaacatg
     3121 aaagcagaaa tcattgctgt tggaacagag attttgacag gacagattgt caacaccaat
     3181 gcccagtttt tgtcggaaaa actagctgag attggggtag atgtatattt tcagacggct
     3241 gtaggagaca atgaagttcg tctcttgtct ttgcttgaga ttgccagtca acgtagcagt
     3301 ctggtgattt tgacaggcgg tttggggcca actgaggacg acctaaccaa acaaacccta
     3361 gctaaatttt tagggaaagc attagtcttt gatcctcagg ctcaggagaa gttggatatc
     3421 ttttttgccc tgcgaccaga ctatgcccga acaccgaata acgaaagaca agctcaaatt
     3481 gtagaaggag cgattccact gccaaacgaa acaggactgg ctgttggagg aaaattagaa
     3541 gtagacggag tgacctatgt cgtccttcca ggtccgccaa gtgaattgaa acccatggtc
     3601 ttaaaccaac ttctacccaa gttgatgaca gggagcaagc tgtattcccg agttcttcgt
     3661 ttctttggga ttggcgagag ccagttggtt acgattttgg ctgatttaat tgataatcag
     3721 atcgatccta ccttggcccc ttatgccaag acaggagaag tcactctacg tctgtcaaca
     3781 aaggctagca gtcaagaaga ggcgaatcaa gcgctggata tcttggaaaa tcaaatcttg
     3841 gactgccaga ctttcgaagg aatttcttta cgagactttt gctatggtta tggggaagaa
     3901 actagtttag ccagcattgt ggtagaagaa ctgaaaaggc aagggaaaac catcgcggct
     3961 gcagagagtt tgacggcagg tcttttccaa gctaccgtgg cgaatttttc tggagtttca
     4021 agtatattta agggtggttt tgtgacctat agcttggagg aaaaatcaag gatgttggat
     4081 attcctgcca agaatttgga agaacatggt gtggtgtctg aatttacagc tcagaagatg
     4141 gctgagcagg cacgaagcaa gacccagtct gattttggaa ttagtttgac tggagtggca
     4201 ggaccagata gcctagaagg acacccagtt gggacagtct tcataggctt ggcgcaagat
     4261 caaggaactg aggttatcaa ggttaatatt ggaggcagaa gccgagcaga tgtacgtcac
     4321 attgcggtta tgcatgcctt taacctagtt cgcaaggctt tattaagtga ctaacttttg
     4381 atataatagt agataggtct gaggatcatt agaatgtagg agaatagaat ggcgaaaaaa
     4441 ccaaaaaaat tagaagaaat ttcaaaaaaa tttggggcag aacgtgaaaa ggccttgaat
     4501 gacgctctta aattgattga gaaagacttt ggtaaaggat caatcatgcg tttgggtgaa
     4561 cgtgcggagc aaaaggtgca agtgatgagc tcaggttctt tagctcttga cattgccctt
     4621 ggctcaggtg gttatcctaa gggacgtatc atcgaaatct atggcccaga gtcatctggt
     4681 aagacaacgg ttgcccttca tgcagttgca caagcgcaaa aagaaggtgg gattgctgcc
     4741 tttatcgatg cggaacatgc ccttgatcca gcttatgctg cggcccttgg tgtcaatatt
     4801 gacgaattgc tcttgtctca accagactca ggagagcaag gtcttgagat tgcgggaaaa
     4861 ttgattgact caggtgcagt tgatcttgtc gtagtcgact cagttgctgc ccttgttcct
     4921 cgtgcggaaa ttgatggaga tatcggagat agccatgttg gtttgcaggc tcgtatgatg
     4981 agccaggcca tgcgtaaact tggcgcctct atcaataaaa ccaaaacaat tgccattttt
     5041 atcaaccaat tgcgtgaaaa agttggagtg atgtttggaa atccagaaac aacaccgggc
     5101 ggacgtgctt tgaaattcta tgcttcagtc cgcttggatg ttcgtggtaa tacacaaatt
     5161 aagggaactg gtgaccaaaa agaaaccaat gtcggtaaag aaactaagat taaggttgta
     5221 aaaaataagg tagctccacc gtttaaggaa gccgtagttg aaattatgta cggagaagga
     5281 atttctaaga ctggtgagct tttgaagatt gcaagcgatt tggatattat caaaaaagca
     5341 ggggcttggt attcttacaa agatgaaaaa attgggcaag gttctgagaa tgctaagaaa
     5401 tacttggcag agcacccaga aatctttgat gaaattgata agcaagtccg ttctaaattt
     5461 ggcttgattg atggagaaga agtttcagaa caagatactg aaaacaaaaa agatgagcca
     5521 aagaaagaag aagcagtgaa tgaagaagtt acgcttgact taggcgatga acttgaaatc
     5581 gaaattgaag aataagctgt taaagcagtg gagaaatccg ctactttttc gtttattttg
     5641 gattttaccc taaagactat tcttatattt agggtaaaaa ctttttgaaa aatcagttgt
     5701 aaaatgaagt gaaatataaa aggtagattt atctgaaatg ctatattttt tagagcaaca
     5761 taaaaataaa atcatattaa tcgtttttga tgcgaatgat tcatacaaat gcttatctga
     5821 agttgaatgt ggttatttct tagaaaactt aagactgtag aaatagactg ttaattgtgg
     5881 aagggagcta ttactaccac tatttcgtat gctattccct gtattgttat cttaatgcca
     5941 taaaggagtt gggtaaaaag tctttaaaat caagaatatg catgtatcta atcattaaaa
     6001 aactttgttg ttatatgttt tataatggta atatttactt tattatcttc ctgaatggaa
     6061 tttgtccatt cttatgaaag atgctaacta aaggaatgct ttgttgtatt cgacctgata
     6121 attgaagaga atatttattt aaacagtcct caaatcaata aaaatgttat aatttgattt
     6181 ttgaaaaata tttgatataa ttgataatac cagcaatcaa attcattcgt aatccgaagc
     6241 gtttacgatg atttcgatag gttgttgaaa acattttaaa cgtttttact ttggcaaaga
     6301 tgttctcaac cttgcttcat aattttattg tatcatattt ttgtttcaca ggaagcctaa
     6361 tgaaaagagg aggaatttta atgaaaatca aaagtaatcg acagacagac tttcgaaccg
     6421 agaaatatac ttgttttggt attcgaaagt acagttttgg agttgcttca gttgcgattg
     6481 caacgggttt gatgtttcta ggaagtggaa ctgtttcagc aaatgagccc acttcaaaag
     6541 aaactggaga ccaagttgtt gttgaaaata ataaatctga tttacttaag gaaaatcaga
     6601 gaacagaatc aaggtatgaa tcacctgcag tgattccagt tacgaattca aaagaatctc
     6661 aaagttcaga aaataataag gaaattcaat cattaccgtc tgagtcaaca acaaattcag
     6721 ttttgaagga tgctgtagaa aatgaagttg aaaaagttga gaagcaacct aataaatcta
     6781 atgtggaaga tagaagtgta tccactgata ctgatgtatc tttaagacga actcgtcgcg
     6841 atcttgattc taatatagta gaaaaagctg gtagtattag tatcacttca ccaaaggaaa
     6901 atacttcaag tgattcatcg catccaaatg gttttagtcc agtaactaag taccatgatg
     6961 ctagtgatgg gtggtatact tttgttcaat tagatcaaca acgtcaaaat cagaatatcc
     7021 gtgtagttga ttcatatgat ggatatgttg tagtatctgc taaatcagaa caaacgtcag
     7081 ctggttttac aactgatggt acgcttaaat tagaatttta taaccgtaat aatgagctgc
     7141 agcaagaaat tatcatggat aaaaatacaa attccttaga atttgtattt aatcgtaata
     7201 agaaattaat tattaatcga actgcgaatt caatttctat tggtgatatg ccgtatacta
     7261 gaaaaactcg taatcagatt gctaacttta atatcttgta taacggtcca gaaatctcga
     7321 ataaacaagg acaaggtcct ttttatgctg atggatggtt gatacctacc aataattcag
     7381 taactgtacg tttcaaggat aaagatacca atagagaatt ggttccttct catacattaa
     7441 atggtttgac agggatgcgt tttgatacta ctgcggcaga attaaagggt tatgaactag
     7501 tagagaaaga gattgttggt tctccaaatg gatatatttc tccttggaag aaagtcggtg
     7561 atagttttac aaaagtaact cagcctaacc tagaggtcaa gtatacattg cttgacatgg
     7621 atggtaccat gaaaacagag gtttactata gtggcaagaa gattcatgaa ataactctta
     7681 aaaaaggaga aacatcctat tataatggta ctgcagctaa tagacgtcca tatactcaag
     7741 ttttagagaa tccattttta gttgcaacta atgaggttgt gtactactat aaggcaacta
     7801 c
//
LOCUS       DAVDWM010000070         7015 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVDWM010000070 DAVDWM010000000
VERSION     DAVDWM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.070"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(1..110)
                     /locus_tag="VUW49_002004"
                     /pseudo
     CDS             complement(1..110)
                     /locus_tag="VUW49_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            96..581
                     /locus_tag="VUW49_002005"
     CDS             96..581
                     /locus_tag="VUW49_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7981865.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            665..1372
                     /locus_tag="VUW49_002006"
     CDS             665..1372
                     /locus_tag="VUW49_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7981866.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            1373..2704
                     /locus_tag="VUW49_002007"
     CDS             1373..2704
                     /locus_tag="VUW49_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET7981867.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            2871..3746
                     /locus_tag="VUW49_002008"
     CDS             2871..3746
                     /locus_tag="VUW49_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET7981868.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            3864..4727
                     /gene="pstC"
                     /locus_tag="VUW49_002009"
     CDS             3864..4727
                     /gene="pstC"
                     /locus_tag="VUW49_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7981869.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            4720..5535
                     /gene="pstA"
                     /locus_tag="VUW49_002010"
     CDS             4720..5535
                     /gene="pstA"
                     /locus_tag="VUW49_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7981870.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            5537..6289
                     /gene="pstB"
                     /locus_tag="VUW49_002011"
     CDS             5537..6289
                     /gene="pstB"
                     /locus_tag="VUW49_002011"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET7981871.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            6304..6954
                     /gene="phoU"
                     /locus_tag="VUW49_002012"
     CDS             6304..6954
                     /gene="phoU"
                     /locus_tag="VUW49_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7981872.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
BASE COUNT         2047 a         1368 c         1449 g         2151 t
ORIGIN      
        1 tgtacttccg tcgcatcaat cattaccgtg tcctcagaac tgagaggagt tcttgaaatc
       61 gtaaaaccac tttgaacaag agttacttca acccattggc tccgacggat aaagaagact
      121 tgataagaac tctagagtta gctccccatg ttgaaggtgg ctattttaga caaacaaaaa
      181 aagcttcaga cactataaac cttgctggta agggaagggc gctttatacc agtatttatt
      241 tcttattaga agaaacgaat ccttcacatt ttcaccgatt gacagcagat gaaatctggt
      301 attttcatgc aggatcccca cttactgttc acatgattac agcagatggt cactacgaag
      361 cagtcacttt aggcctggac atatccaaag ggcaacaact tcactactgt gttcctaagg
      421 gcactatttg gggatcaaca gttgataagg attatgctct ggtttcttgt cttgttgctc
      481 ctggttttga atttgaggat tttgaattat ttgaaagggt agatctactg gcgacctatc
      541 cagagcacaa ggagatgatt gagcgtttga ccagatacta gtcttgataa gcaaatttaa
      601 tctgatttag caatgttagg atttctgata attgtgttat aatgttacca ataggagata
      661 atagatgaca aaacaagtct tattagtgga tgatgaagaa cacattctga aattgcttga
      721 ctaccattta agtaaggaag gcttttctac tcaattggtg acaaatggac ggaaggcctt
      781 agctttggca gaaacagaac cctttgattt tatcttgctt gatatcatgt taccacaatt
      841 agatggcata gaagtttgta agcggctgag agccaaaggc gtcaaaactc caattatgat
      901 ggtttctgcg aaaagtgatg aatttgataa ggttttggcc ttggaattag gggctgatga
      961 ctacctgacc aagcctttta gccctagaga attgctggcg cgtgtcaagg ctgtcctcag
     1021 gcgaactaaa ggagaacaag aaggagatga ttcagataat atcgctgacg attcttggct
     1081 atttgggacc ttgaaagtat accctgagcg tcatgaagtc tacaaggcga ataagttact
     1141 gagtttgacc ccaaaagaat ttgaactctt gctctatctt atgaaacatc ccaacatgac
     1201 actgactaga gagcgtcttt tggaacgtat ctgggggtat gactttgggc aggaaacacg
     1261 tttggtggac gttcatattg gtaagttgag ggaaaaaatt gaagacaatc ctaaagcccc
     1321 tcaatttatt cgaaccattc ggggttatgg ttataagttc aaggagttat agatgaaacg
     1381 ctaccttcaa ttttggctag tcaatctaag tgttagcctc attctgattg cagggatggc
     1441 attaacttgg atcagtaaag gcatcggtct ctttcttcta gccctttctt tgggactagg
     1501 tggctactgg ctcttttgcc tttggaaatg ggaagttgct tttgagacct tgcaccaacc
     1561 tctcttgacc agtagtgaat actttttaga aaaagggcaa gaagacttaa agtctttggc
     1621 tcagtatgtg tctggcctaa aaaccaaagt ctcccaacag gatcagcaat acaaggatct
     1681 agccgaaaca atggaggttc ttctgtcgca cctaaccatg gggacgtttt tagtttccgc
     1741 tcaaggtcag atgttattat ccagtcgctc tctgcctcat tattttcccg atgtggacgg
     1801 cgacataagt tcacttgatg acctcaaacg gatggatatt cggaatttag ttcaccaagc
     1861 ctttgatcaa aaaacaaggt taaaacaaga agtaagtggg tttcatgagg gtgacttgat
     1921 tttagaagtg acagcagttc ccgtttttag ccccacccaa tctgtggaag ctgtgctcgt
     1981 cttgctatat gatttaacaa cgattagaac ttatgaaaag ttaaatttag cctttgtctc
     2041 aaatgcctcc catgaattga ggacaccagt tacttcgatt aagggctttg ctgaaaccat
     2101 taaggggatg tcagctgaag aagaagcgct caaggatgac tttctagaca ttatttacaa
     2161 agaaagtttg cgtcttgagc atattgttga gcatcttctt accttatcta aggctcaaca
     2221 aatgcctata caatggacga ccctttcttt ggcagaattt gtacaggatt tgactcaaag
     2281 cttgcaacct cagctcaaga agaaggattt acagctaaag gtgcaggtgc cagatgatgt
     2341 caccctcgta tcagatagtc aattactttc ccaaatctta ctcaatcttt tatccaatgc
     2401 catccgttac actgaacaag gggggaaaat tgaggtcaag acccaaaagg tgaacgaagg
     2461 cattaagatt tctgtatcag atacagggat tggtattagt caattagagc aggatcgtat
     2521 ttttgaacgt ttttaccgag ttaataaagg tcgaagcaga caaactggtg gcactggtct
     2581 tggccttgcc attgtcaaag aactcagtca attattaggt ggccaagtca cggtgacgag
     2641 tcagcttggc agaggcagtt gcttcacgat ttttcttcct aaccaatctt tcgcacagga
     2701 ctaataattt ttaaagctat ttttaggagc agccattttt caaaagtggc tttttttgat
     2761 tctttacaca atctttacat gaatcaggga taaaccttta caaagacttg ttagactata
     2821 aatgtagtaa gcctacacaa gaaaaataca tagagataaa ggtgattatt atgaaattca
     2881 aaaaaatgct tactcttgca gccattggct tatcaggatt tgggcttgtt gcctgtggca
     2941 atcagtcagc tgcttccaaa cagtcagctt caggaacgat tgaggtgatt tcacgagaaa
     3001 atggctctgg aacacggggt gccttcacag aaatcacagg gattctcaaa aaagacggtg
     3061 ataaaaaaat tgacaacact gccaaaacag ctgtgattca aaatagtaca gaaggtgttc
     3121 tctcagcagt tcaagggaat gctaatgcta tcggctacat ctccttggga tctttaacga
     3181 aatctgtcaa ggctttagag attgatggtg tcaaggctag tcgagacaca gttttagatg
     3241 gtgaataccc tcttcaacgt cccttcaaca ttgtttggtc ttctaatctt tccaagctag
     3301 gtcaagattt tatcagcttt atccactcca aacaaggtca acaagtggtc acagataata
     3361 aatttattga agctaaaacc gaaaccacag aatatacaag ccaacactta tcaggcaagt
     3421 tgtctgttgt aggttccact tcagtatctt ctttaatgga aaaattagca gaagcttata
     3481 aaaaagaaaa tccagaagtt acgattgata ttacctctaa tgggtcttca gcaggtatta
     3541 ccgctgttaa ggagaaaacc gctgatattg gtatggtttc tagggaatta actcctgaag
     3601 aaggtaagag tctcacccat gatgctattg ctttagacgg tattgctgtt gtggtcaata
     3661 atgacaataa ggcaagccaa gtcagtatgg ctgaacttgc agacgttttt agtggcaaat
     3721 taaccacctg ggataagatt aaataaaatg tttgctccat aaatctctaa agagatgcag
     3781 acgtttcatc gtacaataag ataaagaagg caagtaggga ggtgtcgtat ctcccttact
     3841 ttcttcacta gaaaggacaa gatgtgacaa aacaagcctt caaagaagca gtttttaggg
     3901 caattttttt catgagtgca acagtagctg ttgtagctat tttgctaatc tgtttcttta
     3961 tttttagtaa tggcttacct ttcatggcta actacggctt tgcccgtttt ttattaggca
     4021 gtgattggtc gccaacgaac attccggcaa gctatggtat tttaccaatg atcgttggtt
     4081 ccttattaat taccttagga gcgattgtga ttggggtgcc aacaggcatc ttgacatcgg
     4141 tgtttatggt ttattattgt ccaaagcccg tctatggctt cttaaaatca gctatcaact
     4201 tgatggcggc cattccatct attgtttatg gttttttcgg cctacaatta ttggtgcctt
     4261 ggattagaac ctttttagga aatggcatga gtgtcctaac cgcttcgtta ctattaggaa
     4321 taatgatttt gccaaccatt atcagtttgt cagaatctgc tatccgaaca gttcccaaaa
     4381 cgtattattc tggtagcttg gctctaggag ctagtcatga acggagtatt tttagtgtca
     4441 tcttgccagc tgcgagatct ggtattttat cagcagttat tttaggaatc ggtcgcgcag
     4501 taggtgaaac catggcagtt attttggtgg caggcaacca gccgattatt ccaagtggac
     4561 tcttttcagg aaccagaacc ttaacaacca atattgttct ggaaatggct tacgcatcag
     4621 gtcagcatag ggaagccctt attgcaacct cagcagttct ctttttcctt attctcttga
     4681 ttaatgccta ctttgcctac ttgaaaggaa aatcatctta tgagtaaata cctgctaaaa
     4741 cttctcgttt attgtttttc agctttaacc tttggctctc tctttttaat cattggtttt
     4801 atcctcatca aaggcttacc tcatctaagt ctatccctct tttcttggac ttatacttct
     4861 gagaacattt cccttatgcc agcgattatt tccaccgtta ttctggtttt tggtgctctt
     4921 cttttagcct tgcccatagg gatttttgct ggtttttatc ttgtggaata tacaaaaaaa
     4981 gattcccttt gtgttaaaat catgcgattg gcctcagata ccttgtctgg gattccttcc
     5041 attgtttttg gtctgtttgg catgctcttc tttgtagtct tcttaggttt tcaatactct
     5101 ctgttatcag gaatcttaac ctcagttatc atggtgttgc cagtcattat tcgctcaaca
     5161 gaagaagccc ttttatctgt tagtgatagc atgcgtcaag caagttatgg acttggggca
     5221 ggtaagttac ggactgtttt tagaattgtt ctaccagttg ccatgccagg tattttagct
     5281 ggagtgatac tagctattgg ccgtatcgtt ggtgaaacag ctgccctcat gtatacatta
     5341 ggtacctcta ccaatacgcc aagtagtctc atgtcttcag gccgttctct agccctacat
     5401 atgtatatgc tgtcaagtga ggggctacat gtcaatgaag cctatgctac cggtgtgatt
     5461 ttgattatta ctgttttaat gataaatact ctatcaagct tattatctcg aaaacttgtg
     5521 aaaggagctt cctagtatgg gaacattttc agtcagacac ctagacttat tttacgggga
     5581 ttttcaagcc ttaaaaaata tttcgattca attaccagaa agacagatta ctgccttgat
     5641 aggcccatct ggttgtggca aatcaacttt tctaaaaacc cttaaccgga tgaacgattt
     5701 ggttccttct tgccatattg aaggccaagt cctcttagat gagcaagata tttatagtag
     5761 caaattcaac cttaatcagc tacgtaagcg tgtagggatg gtttttcaac agcctaatcc
     5821 ctttgccatg tctatctatg ataacgtggc ttatggccca aggacacatg gtattcgaga
     5881 caaaaaacaa ttagatgcct tagtggagaa atctttaaaa ggggcagcca tttgggaaga
     5941 agtcaaagat gatcttaaaa agagtgccat gtccttatct ggcggtcagc agcaacgcct
     6001 ttgcattgcg cgagctttag cagtagaacc tgatattctg ttaatggatg agccgacttc
     6061 agccttagac cctatctcca ctttaaaaat tgaagacctc attcagcaac taaaaaagga
     6121 ttatacgatt atcattgtta cccataacat gcaacaagct tcacgtattt cagataaaac
     6181 tgcttttttc ttaacaggag aaatttgcga atttggagat accgttgacg tgtttaccaa
     6241 tccaaaagat cagcgcacag aagactatat ttcaggacgg ttcggataag gaaggaaaaa
     6301 cctatgagaa atcaatttga cttagaattg catgaattag aacaatcctt tttaggacta
     6361 gggcaacttg tccttgaaac agcttcaaaa gccttactgg ccttagcctc caaagacaag
     6421 gagatggcag agctaattat caataaggat catgctatca accaaggtca aagcgctatc
     6481 gaattgacct gtgcccgttt gttggccttg cagcagccac aagtgtctga ccttcgattt
     6541 gtgattagca tcatgtcttc ttgttcagac cttgaacgta tgggagacca tatggcaggc
     6601 attgccaaag ctgttttgca actaaaagaa aatcaactag cccctgacga agaacagtta
     6661 caccaaatgg gtaaattatc cctcagcatg ctagccgatt tattggttgc ctttcctttg
     6721 caccaagcct caaaagctat tagtattgct caaaaagatg aacagattga ccaatattat
     6781 tatgccttat caaaggaaat cattggactt atgaaagacc aagaaacctc aattcccaat
     6841 ggaactcaat acctttatat catagggcat ctggaacgct tcgctgatta cattgctaac
     6901 atttgtgaac gcctagtcta cctagaaaca ggagaactag tggatttgaa ttaattcaac
     6961 taatccttaa aagagaagag tacgattaag tgctcttttt tatggttgta aaaaa
//
LOCUS       DAVDWM010000071         6761 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVDWM010000071 DAVDWM010000000
VERSION     DAVDWM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.071"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..961
                     /locus_tag="VUW49_002013"
     CDS             <1..961
                     /locus_tag="VUW49_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7981873.1"
                     /translation="KENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIRLYNNPKKNQ
                     ASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRPTIVEGKGKW
                     AETLHLVEENMFGSSIANYRNSAEDRYVSSIFHGMYKREIFQKVGLVNEQLDRTEDND
                     IHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQFKCLSLFHY
                     VPCLFVLSLVFSLALLPITLVFITLLLGAYFLLLSLLTLLTLLKHKNGFLIVMPFLLF
                     SIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            1000..2850
                     /locus_tag="VUW49_002014"
     CDS             1000..2850
                     /locus_tag="VUW49_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET7981874.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDILSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLTFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSILS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDTKKKGQRLGGIPVLGSYDNLSELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(3122..3220)
                     /locus_tag="VUW49_002015"
                     /pseudo
     CDS             complement(3122..3220)
                     /locus_tag="VUW49_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(3246..3878)
                     /locus_tag="VUW49_002016"
     CDS             complement(3246..3878)
                     /locus_tag="VUW49_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7981875.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLVPQQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(3900..4772)
                     /gene="sdaAA"
                     /locus_tag="VUW49_002017"
     CDS             complement(3900..4772)
                     /gene="sdaAA"
                     /locus_tag="VUW49_002017"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET7981876.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(4781..5452)
                     /gene="sdaAB"
                     /locus_tag="VUW49_002018"
     CDS             complement(4781..5452)
                     /gene="sdaAB"
                     /locus_tag="VUW49_002018"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET7981877.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHFHN
                     VNFFK"
     gene            5694..6281
                     /locus_tag="VUW49_002019"
     CDS             5694..6281
                     /locus_tag="VUW49_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7981878.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(6334..6678)
                     /locus_tag="VUW49_002020"
     CDS             complement(6334..6678)
                     /locus_tag="VUW49_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET7981879.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLK
                     KEVEIYYQAKER"
BASE COUNT         1977 a         1285 c         1438 g         2061 t
ORIGIN      
        1 taaagagaat attgaaattc tatttataaa tgctatgtcc accgatggga ccacagctat
       61 cattcagcaa tttataaagg aagatacaga gtttaactca attagattgt ataacaatcc
      121 taagaaaaat caagctagtg gttttaacct gggagttaaa cattctgtag gggaccttat
      181 tttaaaaatt gatgctcatt caaaagttac tgagactttt gtaatgaaca atgtggctat
      241 tattcagcaa ggtgaatttg tctgtggggg gcctagaccg acgattgtcg aaggaaaagg
      301 aaaatgggca gagaccttgc atcttgttga ggaaaatatg tttggcagta gcattgccaa
      361 ttatcgaaat agtgctgagg atagatatgt ttcttctatt tttcatggga tgtataaacg
      421 agagattttc cagaaggttg gtttagtaaa tgagcaactt gaccgaactg aagataatga
      481 tattcattat agaattcgag aacatggtta taaaatccgc tatagcccaa gtattctatc
      541 ttatcagtat attcgaccaa cattcaagaa aatgctgcat caaaagtatt caaatggttt
      601 gtggattggc ttgacaagtc atgttcagtt taagtgttta tcattatttc actatgttcc
      661 ttgtttattt gttttgagtc ttgtgtttag tctagcattg ttaccgatca cattggtatt
      721 cataacttta ctattaggtg cctattttct acttttgtca ttactcactt tgctgacttt
      781 attaaaacat aaaaatggat ttctaattgt gatgcccttt cttttatttt ccattcactt
      841 tgcttatggc tttgggacga ttgtaggttt aattagagga tttaaatgga agaaggagta
      901 caagggaaca ataatttatt tggataaaat aagccaaata aatcaaaata tgctacaata
      961 acaatatagt agaactattt taaggaggag taaatttcta tgaataaaaa actaacagat
     1021 tatgtgattg atctggtgga aattttaaat aaacaacaaa aacaggtttt ctggggaata
     1081 tttgatattt tgagtatggt ggtttccatc attgtatctt atatcttatt ttatggcttg
     1141 attaatccag cacctgttga ctacattatc tatacgagtt tgaccttcct gttctatcaa
     1201 ttgatgattg gtttttgggg cttgaacgcg agtattagtc gttacagcaa gattacggat
     1261 ttcatgaaaa tcttttttgg tgtgactgcc agcagtatcc tgtcatatag tatctgctat
     1321 gccttcttgc cactcttctc catccgtttc atcattctct ttatcttgtt gagtaccttc
     1381 ttgattttat tgccacggat tacttggcag ttaatctact ccagacgcaa aaaaggtagt
     1441 ggtgatggag aacaccgtcg taccttcttg attggtgctg gtgatggtgg ggctctcttt
     1501 atggatagct accaacatcc aactagtgaa ttggaacttg ttggtatttt ggataaggat
     1561 actaagaaaa agggtcaaag acttggtggt atccctgttt taggctctta tgacaatctg
     1621 tctgaattag ccaaacgcca tcaaatcgag cgtgtcattg ttgcgattcc gtcgctggat
     1681 ccgtcagaat atgagcgtat cttgcagatg tgtaataagc tgggtgtcaa atgttacaag
     1741 atgcctaagg ttgaaactgt tgttcagggc cttcaccaag caggtactgg cttccaaaaa
     1801 attgatatta cggacctttt gggtcgtcag gaaatccgtc ttgacgaatc gcgtctgggt
     1861 gcagaactga caggtaagac catcttagtc acaggagctg gaggttcaat cggttctgaa
     1921 atctgtcgtc aagttagtcg cttcaatcct gaacgcattg tcttgctcgg tcatggggaa
     1981 aactcaatct accttgttta tcatgaattg attcgtaagt tccaagggat tgattatgta
     2041 cctgtgattg cggacattca agactatgat cgtttgttgc aagtctttga gcagtacaaa
     2101 cctgctattg tttatcatgc ggcagcccac aagcatgttc ctatgatgga gcgcaatcca
     2161 aaagaagcct tcaaaaacaa tatccgtgga acttacaatg ttgctaaggc tgttgatgaa
     2221 gctaaagtgt ctaagatggt tatgatttcg acagataagg cagttaatcc accaaatgtt
     2281 atgggagcaa ccaagcgcgt ggcggagttg attgtcactg gctttaacca acgtagccaa
     2341 tcaacctact gtgcagttcg ttttgggaat gttcttggta gccgtggtag tgtcattcca
     2401 gtctttgaac gtcagattgc tgaaggtggg cctgtaacgg tgacagactt ccgcatgacc
     2461 cgttacttta tgaccattcc agaagctagc cgtctggtta tccatgctgg tgcttatgcc
     2521 aaagatgggg aagtctttat ccttgatatg ggcaaaccag tcaagattta tgacttggct
     2581 aagaagatgg tgcttctaag tggccatact gaaagtgaaa ttccaatcgt tgaagttgga
     2641 atccgcccag gtgaaaaact ctacgaagaa ctcttggtat caaccgaact cgttgacaat
     2701 caagttatgg ataagatttt cgttggtaag gttaatgtca tgcctttaga atccatcaat
     2761 caaaagattg gagagttccg cactctcagt ggagatgagt tgaagcaagc tattatcgcc
     2821 tttgctaatc aaacaaccca cattgaataa aaaagaaaaa cgcatattac caagttaaac
     2881 aaccttggta atatgcgttt tattatgtag agacttatac tcttcgaaaa tctcttcaaa
     2941 ccacgtcaac gtcgccttgc cgtatgtata tgttactgac ttcgtcagtt ctatctgcaa
     3001 ccataaaaca gtgttttgag ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     3061 ttgagctgac ttcgtcagtt ccatccacaa ccataaaaca gtgttttgag ctgacttcgt
     3121 cagttccatc tacaacctta aaacagtgtt ttgagcagcc cgcagctagt ttcctagttt
     3181 gctctttgat tttcattgag tattacttca ttttcttctg aaatggaatt gttacccagt
     3241 ctatgctatt gaaaatacgc caaaacctct aagggtttgt gagcgatata atcaggttga
     3301 tagtttagta gatctgcttg ctctccaaat ccccaagtga cggccaattt ctgaatacct
     3361 gtttctcgag ctcccagcat atcaaacttg gtatctccga tgatgatggc ttgttgagga
     3421 actagttgat gtgtctgcaa ggcttggtga atgacatctg ccttatgggg tgcttcaggg
     3481 ctagaaccat aaatgccatc aaagaaatga tggatttcca agttttttgc catgtcttga
     3541 gcagtagatg tattctttgt cgtggtgatg tagagtggat aactgctcga taactcctca
     3601 agcaagtcta taatctgagg aaagagttga gcttcataga tgccttttgc cttatagtaa
     3661 gaacgatata tctgcacggc ttcagaaatt tggtctttgg acaggcaggt cgcaaaacta
     3721 ctttcgagag gtggtcccat aaaaccacga atagttttgg catcagggct aggcaccccc
     3781 agctctttaa aggtataggt aaaggcattg tgaatcccga tagaactatc aacgagggtt
     3841 ccatccaaat cgaaaaaaat cgctgtgata gaggtcatgg tttctcctat ttgataagct
     3901 tattctccga aaatttcttt ttggaggcga cgaccagtag gggtggtagc gagtccacct
     3961 tcagctgttt cacgaaaggc agttggcatg cttgctccta cttggtacat ggcatcgatc
     4021 acttcatcca cagggatttt agattcgata cctgccaagg ccatgtctgc tgcgatgaaa
     4081 gcaaagctag ctcccatggc attacgtttg acacagggaa cttcgaccaa acctgcaaca
     4141 gggtcacaga tgaggcctag catattttta atgacaaagg caatagcttg actggcctga
     4201 taaggtgttc cacctgcagc cagagtcaag gcggcagcac tcatagcaga ggctgaacca
     4261 acttcagctt gacacccacc ctcagcacct gagatggagg cattgtttgc gatgactagt
     4321 ccaaaggcac cagcagcaaa gaggaaatcc aattgttgct cgtggctgag gtctaatttt
     4381 tcaatagcag cagtgagaac ggatggcaga cagccagcac ttccagcggt tggagtggca
     4441 cagaccaagc ccattttggc attgtgttca ttgactgcga tggcatttcg ggcagccgag
     4501 agaatcgtat aatctgacag agtttttccg ttttcgatgt agtgatccaa tttggcagca
     4561 tctccacctg tcaggccact acgagattta ttttcattga ggccaagttg gacagaggct
     4621 ttcataactt ccagattgcg ttccatgaga aggaagactt cttcacgttc gcgaccggtc
     4681 aattcaaact ctgttgtaat catgagttct gcgacatttc cttgaaagtc cagatctgct
     4741 tgctcgacca attctttgat agaataaaac atgcttcctc ctatttaaag aaattgacat
     4801 tgtggaaatg agggattttt cgaatttctt cgatagcctc atcacagttg cgactgtcaa
     4861 cttcgataat cataatggct ttttcaccag ctttttcacg agtgacattc atctgggcga
     4921 tattgatacc atagcgggaa agcgcctctg taacaagggc aatcatacct ggaatatctt
     4981 gatgaacgat gatgatagtc ggtgtattca tattgagaga gacggcaaaa ccattgagtt
     5041 cggttacctg aatatttcct ccaccgatag aaataccagt cacgctgatg gtcttgtggg
     5101 catttttaac agtaatttta gtggtattag ggtgaggggc attgctgtct ttctgaatgg
     5161 tccagacaat cttgatacca cgcttgtggg caatttccag actatttgga atttcaggat
     5221 catctgtatc cattcctaaa atacctgcaa caagggctag gtctgttccg tgaccacgat
     5281 aggtcttggc aaatgagtta aaaagttgga attcaacttc tgtcggagta tcatcaaaaa
     5341 tggaagagac aatcttccca atacgaacag caccagcggt atggctacta gatgggccaa
     5401 tcataactgg tccgatgata tcaaagacag attgaaaacg aagtgatttc atcagtttcc
     5461 ccttataaaa attcttatct ctattatatc aaagaatgag gggcttggct ttaattgtgg
     5521 atgaaaacct ttctaatacc tcaaatagca taaaaatagt atcttttatg acaaaaaaca
     5581 ccttatttag ggaaataaaa aataattttg taatatttct acataaaagt gtcaagaaac
     5641 ggtaatattt aaagggtatg atagaactat agaaagaagg agaattttcg aatatgaaat
     5701 caataactaa aaagattaaa gcaactcttg caggagtagc tgccttgttt gcagtatttg
     5761 ctccatcatt tgtatctgct caagaatcat caacttacac tgttaaagaa ggtgatacac
     5821 tttcagaaat cgctgaaact cacaacacaa cagttgaaaa attggcagaa aacaaccaca
     5881 ttgataacat tcatttgatt tatgttggtc aagagttggt tatcgatggc cctgtagcgc
     5941 ctgttgcaac accagcgcca gctacttatg cggcaccagc cgctcaagat gaaactgttt
     6001 cagctccagt agcagaaact ccagtagtaa gtgaaacagt tgtttcaact gtaagcggat
     6061 ctgaagcaga agccaaagaa tggatcgctc aaaaagaatc aggtggtagc tatacagcta
     6121 caaatggacg ttatatcgga cgttaccaat taacagattc atacctgaac ggtgactact
     6181 cagctgaaaa ccaagaacgt gtagcagatg cctacgttgc aggacgttac ggttcatgga
     6241 ctgctgctaa aaacttctgg cttaacaatg gctggtatta agaactaacg aacaaataat
     6301 atgtaaagag tcgaggaaat ccctcggctt tttttatctt tccttcgctt ggtaatagat
     6361 ctctacttct tttttgagat gagacagcat agaagtttcc tcggttagct ccagaaggga
     6421 gaaccctttt tggataagtt tagaatcgtc tgtacaaatc ccaatacgga catatgtaca
     6481 aatcccaata cggacatagc agatagccag cctatcgtag cttttcttat tcttggcgtc
     6541 ctctagtaaa gtgtagcaga tttgcttaca catgttttta tcttgattgt atagatagag
     6601 tgtggaaagg tttaatagaa ttgtcattcg caagtcatat aaatgttgat agtttttata
     6661 gacttctaag cgttgcaaga ttttctcgga ttattgacaa ataaacggac attctgatag
     6721 acgattgccc tagcggctta gtgtaaactt tacaaatttg g
//
LOCUS       DAVDWM010000072         6746 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVDWM010000072 DAVDWM010000000
VERSION     DAVDWM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.072"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..220
                     /locus_tag="VUW49_002021"
     CDS             <1..220
                     /locus_tag="VUW49_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7981880.1"
                     /translation="KEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSV
                     KPQELDWEFIGKNIDSWIEKAQLEFVEQ"
     gene            237..1241
                     /locus_tag="VUW49_002022"
     CDS             237..1241
                     /locus_tag="VUW49_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7981881.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSETQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIEEKVTMYINPRDIMQF
                     "
     gene            1253..2947
                     /locus_tag="VUW49_002023"
     CDS             1253..2947
                     /locus_tag="VUW49_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7981882.1"
                     /translation="MRKSLNWKDWLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTFAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFHPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNVPHLILFNLVLVGTVIILLIAYTIQKLPFSYRMIR
                     AVFFSIDNDMEEAARSMGASSFYTMVRVIIPYILPVVLSVVVLNFNSLLSDYDLSVFL
                     YHPLFQPLGIIIKQSTDETATLNAQAMMFVYSVILMIMSSAALYLSSLFQGKRGKR"
     gene            2949..3650
                     /locus_tag="VUW49_002024"
     CDS             2949..3650
                     /locus_tag="VUW49_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7981883.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSLEG
                     YNRLLIYLNTSGIKILEIQNGIGNLDEERQLNKIKSTKVSCYLSLKLNENFEHLKLME
                     EMHNIAGVIYVEEIR"
     gene            3697..4296
                     /locus_tag="VUW49_002025"
     CDS             3697..4296
                     /locus_tag="VUW49_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7981884.1"
                     /translation="MNIIFDLGNVLIEWNKEKILSKICKNDLEYNLFNQFVFQSNLWI
                     DLDNGKISLEFLENQLIDEMGHQYQDQIHELVWNWFNYVDLYDEVYELIKQLKKKNFQ
                     IYVLSNTSSIFHILLDSVLSKVSSVLDGYVISCEVKMMKPQKEIYLSLVNKYQLDIKD
                     CIFLDDLEENVEAARTLGIKAFQIKERKEISNILKDLLN"
     gene            4306..6333
                     /locus_tag="VUW49_002026"
     CDS             4306..6333
                     /locus_tag="VUW49_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7981885.1"
                     /translation="MVTIKDIARVSGVSHGTVSNVLNKKGNVSAAKIKLVEETAKKLG
                     YNMNSQAQFLRKGASSKCFFLLFNNARKKYAEYFAEIDQLSLDVEYVYLHKFSEIKNK
                     ISAILSENPQFIVCLGFSPKRFVDIEDNIPIFEVDTYQSNKTLSSFDSDKIFEIVLEL
                     VRTEKLSNLYFISLTNEKEHSLFEYLTQKIKNSTHIQVQERLQLLSLYPTLKNFAKYD
                     CIFVDDEDLMHTLLDLFDWFTIIDRPKICLIGSRVWFEKRNLFYIHLDYRILALNTAE
                     NLESGNSSIVPVKDGKQILTNQLFDGECDDIYLLTVHSPMNKALEILSEKYKQISGKN
                     IKIIEKRYDELLEMIESGDVSSSFDMIRMDMAWLPTFGKKYFQEITSFRQTKSINQNI
                     SKSVSREYMYIDQKQYTFPLDVSSQILVYRKDLFEDSFLQRRYFEKTKRKLTVPKSYQ
                     EFDELSEFFTLTENPFSPTLYGHSLALSSSVRAACEFIPRFRERLAQSRMNLAVLPQV
                     LTEYEKSYQFTEKSFNSSWNDFVTNLNSGKTSMEIIFSNYTSPLFDGLNVSAQFEFAT
                     ATIPGNTPVIGGGSIGISKYSNRVEECLNFINWLYSEEISILLTSLGGFLPSKYVMQN
                     RMLQFQYPWLSSLEDTFSIGSRTKWYGFNTDFRFEQMLGQELIESVKHRYG"
     gene            6492..6746
                     /locus_tag="VUW49_002027"
                     /pseudo
     CDS             6492..6746
                     /locus_tag="VUW49_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2309 a          896 c         1191 g         2350 t
ORIGIN      
        1 aaaagaattt gtagattggt ttggctcttc agaacttcaa aaagaatggt ctgataaatt
       61 tggttcaatt ccagctaata aaaaagctct agagcaagca aaagatgaac tcaaacagtt
      121 tgctaatagt gttaaaccac aagaacttga ctgggagttt atcgggaaaa acatcgattc
      181 ttggattgaa aaagcacagc ttgaatttgt tgaacaataa tagttaggat taagtgatga
      241 ttacatttaa ggatattcaa attaagtata atgattttat tgccatcgat aatctcaatt
      301 taacaatcaa tgagggagag tttttcacat ttttaggacc aagtggttgt gggaaatcaa
      361 caactcttcg tgcactagtt ggatttttgg agccttcaca ggggcaaata ctagttgata
      421 atcaagatat tacgaattta gatccggaaa aacgcgaaat tggtattgtg tttcaaagtt
      481 atgcattatt cccaacaatg actgtttttg ataatattgc ttttggtcta aaagttaaaa
      541 aagtttctcc tgaaattatt aaagctaaag ttcatgaaat tgcgaaaaaa attaatatct
      601 cagacgagca actaaaccga aatatttctg aattatcagg gggacaacaa cagcgtgtag
      661 ctttagcacg tgcattagta ttagaaccaa aaattctttg tttggatgaa ccgttatcaa
      721 acctagatgc caaactacga gtttcgttga gaaaagaatt gaaacgattg caaaaagaac
      781 taggaatcac tactctttat gtcacacatg atcaagaaga agcattaaca ttatcagata
      841 ggattgctgt gtttaataat ggatttattg agcaagttgg cacgccgtat gatatttata
      901 atcaatccga aactcaattt gtctgtgatt ttattggctc aatcaataaa attagccaag
      961 aagttcttaa aaaattacaa ggcgagaata aatactttaa agaaactgaa ggatacatac
     1021 gcttagagcg tgttaagtta gagaaaactt cacctcaaga tttatctata acaggagtaa
     1081 ttagtgatat tgagtttaat ggtgtactta ttaagtataa agtagacttg tcgaatagtg
     1141 aaagtattca tattatcgaa aagaatgatg gactatcacg tcatataatc gaagaaaaag
     1201 tcactatgta cattaatccg cgtgatataa tgcaatttta aggagattgt gcatgagaaa
     1261 gagtctaaac tggaaagatt ggctcataag attaggagtt ttatggctaa tctttgccct
     1321 gattatttat ccaaatattg aacttttgtc tggagtgttt tataaaaatg gttcatttac
     1381 tctagaggca ttcaataaga ttatacactc tcagagagcg atgagtagta tatttaatag
     1441 ctttattttg gcgatttcaa tggtctttac tgtaaatatt gttgggacat tgattgttct
     1501 actcacagag tactgggata ttaggggttc aaaaatactt aaattaggtt atatgagctc
     1561 tctagtatat ggaggaattg tattagcgac aggatataaa tttgtttatg gcgctaatgg
     1621 tcttgtaact aaaacgctct caagtataat tccaggtctt gcccctaact ggtttcaagg
     1681 atttggtgca gtattgttta ttatgacatt tgcttgtacc tcaaatcaca taatgttttt
     1741 aacaaatgca gttcgtagtg ttgattatca tacaattgaa gcagctaaaa atatgggtgc
     1801 aagtccttta aaagttttct ttagtgtggt ttttccaact tttaaaccta cgttatttgc
     1861 cttaaccatc ttaacttttt tgacaggtct ttcagcagtt tctgctcctt tgatagtagg
     1921 ggggaaagat tttcaaacta ttaacccatt gattatcagt ttcgctaaaa ctacaagttc
     1981 gagagattta gcagctttac tagctatcat gttaggttta gcaactattc tattattgac
     2041 acttttgaat catgtcgaaa aaggaggtaa ttatgtatct gtttcgaaaa ccaaggctag
     2101 tattaagaag cagataattt cttctccatt atggaatact tttgctcaca ttgttgcgta
     2161 tgtattgttt gcaatctata tgttaccaat agttttgata attctatact cgtttacaga
     2221 ctcgttgacg atcaagacgg gaactttaga tctatctaaa tttacgttag cgaactatgc
     2281 taatttattt acagatgctc aatcatttca tccttaccta gtcagcgttg tttatgcaat
     2341 tttagctgcg gtaattgcta caattatagc aatagtaatt tcacgtattg tacataaaaa
     2401 caagagtaag ttcgataaat tctttgagta tggtgcactg ataccatgga tattgccagg
     2461 tacattgatt gcactaggat taatgttcac ttataatgta cctcatttaa tactatttaa
     2521 tttagtttta gtaggaacag taattatttt acttattgca tatactattc aaaaattgcc
     2581 attttcttat agaatgattc gggctgtatt tttcagtata gataatgata tggaagaagc
     2641 tgcacgtagt atgggtgcat ctagtttcta cactatggtg cgagttatta taccgtatat
     2701 tttaccagtc gtattatcag tagttgtatt aaactttaat tctttattat ctgactatga
     2761 cttatctgta ttcttgtacc atccactttt ccaaccattg ggaatcatta ttaaacagtc
     2821 tactgatgaa acagcaactt taaatgcaca ggcaatgatg tttgtgtatt ctgtaatttt
     2881 aatgattatg tctagtgcag ctctttattt atcaagccta ttccaaggta aacgaggtaa
     2941 gagataatat gaatctttca tacgaactga gtatttggga aatacttctt aggtgctttt
     3001 tagcgattct tttcggtggc attgttggct atgagcgagg agtcaaaaac caagcagcag
     3061 ggattagaac ctacttacta gtttgtttgg gaacttgtgt tgtaatgatg acaaatcagt
     3121 tcatcactag tcgttatggt agcggtgatc ctagtcgaat gggagcccaa gtaattagtg
     3181 gcttgggttt cctaggggca ggaacaatca tggtaactaa tcaaaaagtt aaaggtttaa
     3241 caacagcagc tggattatgg tcttcagcaa ctattggttt agctataggc attggttttt
     3301 atgaaggtgc tattttagcc ggagttgtac tcatagcagt tttaactcta tttactcctc
     3361 ttaaagatta tttaaactcg aaaactaaaa ttgctgatta ctacttggtt tttagttctt
     3421 tagaaggtta taatcgttta cttatatatt taaatacatc gggtattaag attttagaaa
     3481 ttcaaaatgg tataggaaat cttgatgaag aacgtcaatt aaataaaatt aagtcaacaa
     3541 aagtgtcctg ctatttatca ttaaaattaa acgaaaactt tgaacattta aaattaatgg
     3601 aagaaatgca taatattgct ggtgttattt atgttgaaga gattaggtaa gggtgacttt
     3661 gtctaatttt ttctatattt ctaatcggag aaaaaaatga atataatttt tgatttaggg
     3721 aatgtgttga ttgaatggaa taaagaaaaa attttatcaa aaatctgtaa gaatgattta
     3781 gaatataatt tatttaatca gtttgtattt caatctaact tgtggattga tttggataat
     3841 ggtaaaatta gtttagagtt cttagagaat caattaatcg atgaaatggg acatcaatat
     3901 caggatcaaa ttcatgagtt agtatggaat tggtttaact atgttgattt atatgatgaa
     3961 gtttatgaat taataaaaca gctaaagaaa aagaattttc aaatttatgt tttatcaaat
     4021 acctcctcaa tttttcatat tttattagat tctgttctat ctaaagtatc atctgtttta
     4081 gatggttacg taatttcttg tgaagttaaa atgatgaaac cacaaaaaga aatttacttg
     4141 tcattagtta ataaatatca attagatata aaagattgta tatttttaga tgatttagaa
     4201 gaaaatgtag aagctgcaag aactctaggt attaaagcat ttcaaattaa agagcgtaaa
     4261 gagatctcaa atatactaaa agatctctta aactagaggt taattatggt tacaataaaa
     4321 gatattgcaa gagtgtctgg agtctcacat gggacagttt caaacgtttt aaataaaaaa
     4381 ggtaatgtct cagcagctaa aataaagtta gtagaagaga ctgcgaaaaa attgggttat
     4441 aatatgaatt ctcaagctca gtttcttcga aaaggcgctt ctagtaaatg ttttttcttg
     4501 ttatttaata atgcacgcaa aaagtatgct gagtattttg cagaaattga tcagttatct
     4561 ttagatgtag aatatgttta cttacataaa ttctcagaga ttaaaaataa gatatctgca
     4621 attttaagtg aaaatcctca atttatagtt tgcttaggtt tttctccaaa aagattcgtt
     4681 gatattgaag ataatattcc gatttttgaa gtagatacat atcaatctaa taaaacttta
     4741 tcttcctttg atagtgataa aatatttgag attgtacttg agttggttcg gactgaaaag
     4801 ctatctaatt tatattttat ttcacttact aatgaaaaag aacattcttt atttgaatat
     4861 ttgactcaaa aaataaaaaa ctcaactcat atacaagtgc aagaacgttt acaattgttg
     4921 tctctatatc ctactctaaa aaactttgca aagtatgatt gtatatttgt tgatgatgaa
     4981 gatttgatgc acacgctatt agacttattt gattggttta ctataattga tagaccaaaa
     5041 atatgtttga taggttcacg tgtttggttc gaaaaaagaa atctttttta tatacattta
     5101 gattatagaa tattggcact caatactgcc gaaaatttag aaagtggaaa tagttcaatt
     5161 gtaccagtta aagatggcaa acaaatttta actaatcaat tatttgatgg agaatgtgat
     5221 gatatttatt tacttactgt ccattcacca atgaataaag ccttagaaat tttatcagaa
     5281 aagtataaac aaatttctgg gaaaaatatt aaaattatcg agaaaagata tgatgaactt
     5341 ttggagatga ttgagtctgg cgatgtttca tccagttttg atatgattag aatggatatg
     5401 gcttggcttc caacatttgg gaaaaagtat tttcaagaga ttacaagctt tagacaaaca
     5461 aaaagtatta atcaaaatat ttctaagtcg gtgtctcgag agtatatgta tattgatcag
     5521 aagcaatata cttttccatt ggatgttagt agtcagattt tagtatatag aaaagactta
     5581 tttgaagatt catttttaca aagacgatac tttgaaaaaa caaaaagaaa attaactgta
     5641 ccaaaaagtt atcaagagtt tgatgagtta agtgaatttt ttacattaac agaaaatcca
     5701 ttttcaccta cactttatgg ccatagttta gctttaagtt cttcagtgag agcagcatgt
     5761 gaatttattc ctcggtttcg tgagagatta gcacaatcta gaatgaatct tgctgtactt
     5821 cctcaggttt taaccgagta tgagaaaagt tatcagttta ccgaaaaatc atttaattct
     5881 tcgtggaacg atttcgttac gaacttaaac agtggtaaaa cttcaatgga aataattttt
     5941 tcaaattata ctagtccatt atttgatggt ctcaatgttt ctgctcagtt tgagtttgca
     6001 actgccacta tacctggtaa tactcctgtg ataggtggtg gctcaattgg aatttctaaa
     6061 tattcaaata gagtggaaga atgtctaaac tttattaatt ggttatattc tgaagagatt
     6121 tctattttac taacatctct gggtggtttt ttaccatcaa agtatgtcat gcaaaatagg
     6181 atgttacaat ttcaatatcc atggttatct agtttagaag atacatttag tattgggagt
     6241 agaacaaaat ggtacggctt taatactgat tttagatttg agcagatgtt agggcaagag
     6301 ttaatagaaa gtgtgaagca tagatatgga taaattaata ctaaattttt attactagtt
     6361 tatttattat tgtttaaaat taaagattca agtaaacacc aattatattt aaaattatta
     6421 ttttggcatt tttctattgc aaagttattt tattagactc cctgcgaaac aaaatatggt
     6481 atagtagttc tatgaatgat gaagcaagta aacaattaac tgatacacga tttaagcgtc
     6541 ttgttggtgt tcagcgtacc acttttgaag agatattagc tgtattaaaa acagcttatc
     6601 aaaaaagtcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag
     6661 atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagccgct aacagttgaa
     6721 gataaagccc gtaaccatgc gctatc
//
LOCUS       DAVDWM010000073         6738 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVDWM010000073 DAVDWM010000000
VERSION     DAVDWM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.073"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..304
                     /locus_tag="VUW49_002028"
     CDS             <1..304
                     /locus_tag="VUW49_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981886.1"
                     /translation="PMTYEETMTSDFFEAWFQNFLLPTLTTPSVIIMDNVRFHRMGKL
                     ELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            365..1327
                     /locus_tag="VUW49_002029"
     CDS             365..1327
                     /locus_tag="VUW49_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7981887.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYEDTSISVSSNETDKV
                     LAGSLYTDTNEQGLMIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1504..2511
                     /gene="gap"
                     /locus_tag="VUW49_002030"
     CDS             1504..2511
                     /gene="gap"
                     /locus_tag="VUW49_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7981888.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2579..3415)
                     /locus_tag="VUW49_002031"
     CDS             complement(2579..3415)
                     /locus_tag="VUW49_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7981889.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3541..5736
                     /locus_tag="VUW49_002032"
     CDS             3541..5736
                     /locus_tag="VUW49_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET7981890.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5789..5941
                     /gene="rpmG"
                     /locus_tag="VUW49_002033"
     CDS             5789..5941
                     /gene="rpmG"
                     /locus_tag="VUW49_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7981891.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5951..6127
                     /gene="secE"
                     /locus_tag="VUW49_002034"
     CDS             5951..6127
                     /gene="secE"
                     /locus_tag="VUW49_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET7981892.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6182..6718
                     /gene="nusG"
                     /locus_tag="VUW49_002035"
     CDS             6182..6718
                     /gene="nusG"
                     /locus_tag="VUW49_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET7981893.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2087 a         1185 c         1458 g         2008 t
ORIGIN      
        1 tccaatgact tacgaagaga cgatgacgag cgactttttt gaagcttggt ttcagaattt
       61 tctcttacca acattaacca caccatcggt tattattatg gataatgtaa gattccatag
      121 aatggggaag ctagaacttt tatgcgaaga gtttgggcat aaactgttac ctcttcctcc
      181 ctactcgcct gagtacaatc ctattgagaa aacatgggct catatcaaaa agcacctcaa
      241 aaaggtatta ccaagttgca atacctttta tgaggctctt ttgtcttgtt cttgtttcaa
      301 ttgactatag ttcacggata cagttgggaa agaagctatt aattatttgg agagtccttt
      361 tgggatgaac atgtcaaata ttactataga tgggcaagaa atggatctga gtcaaacgat
      421 gccttcttat gctcaagtag tgagtcttta tgaggataca agcatctctg ttagtagtaa
      481 tgagacggac aaagtgttgg ctggatccct ctatactgat acaaatgaac agggattaat
      541 gattccaagc agtttactaa aaaattggaa tgaacagaca ggaaaaaatt tgacagctaa
      601 tgatcttatt ggcaaatcag tctcagccag cattgtagaa agtgctgccg aagctagtaa
      661 gattgctcaa tttcaaacga agattgtacg tgtaatcaat gatgaagatg acatggagga
      721 cagcaacagt ttcatgctgt ctcatcaaat ggaaacgatt ttgaaagagg ctggatttac
      781 gaaagctgta tcttatttta tcttggaact caaagatcca tcacagacaa aagtagtaac
      841 agaagaatta cagaaaaata agaagtatac tgtgctttct caacagagag ttcttgatat
      901 tgtgattacc tttattcgtg ttattcaggg attattgatt gtgctttcat cacaagctat
      961 tgtggtagca gcggttatga ttggtatcat tatttacatc aatatcatgc aacgttccaa
     1021 ggaaataggt gtcatgaaag cagttggtta tcagaatcgt ggtgtcaaag gaatttttat
     1081 ttacgaggct atctggattg taggcatcgc cttgctgctg gcatttttgg ttgcacaagg
     1141 ggtgggaagt ttggcgaatg cgattgtaag tcacttttac ccatccatca ctaaggtttt
     1201 tgaattaaat cttttatctg ttttaggaac tctagttttc gctctattac ttggttatgt
     1261 ctcagcctac ttcccggcgc gtaagattag taaaatggat cctgtagaat cgttacgcta
     1321 tgaataataa gggattcctt gatttacagt cttaaaaaga aactcacata gaaaaacaat
     1381 ggccatactt gcaattcaac tcaaatagtt gtataatagg tttgttgaaa ggtgatttgt
     1441 agtgattcaa gttactcttt tcacaataaa aatttcatga ttttcataag gaggaaatca
     1501 ctaatggtag ttaaagttgg tattaacggt ttcggacgta tcggtcgtct tgctttccgt
     1561 cgtatccaaa acgtagaagg tgttgaagtt acacgcatca acgaccttac agatccagtt
     1621 atgcttgcac acttgttgaa atacgacaca actcaaggtc gtttcgacgg tactgttgaa
     1681 gttaaagaag gtggatttga agttaacggt aaattcatca aagtttctgc tgaacgtgat
     1741 ccagaacaaa tcgactgggc tactgacggt gtagaaatcg ttcttgaagc tactggtttc
     1801 tttgctaaga aagaagcagc tgaaaaacac cttaaaggtg gagctaaaaa agttgttatc
     1861 actgctcctg gtggaaacga cgttaaaaca gttgtattca acactaacca cgacgttctt
     1921 gacggtactg aaacagttat ctcaggtgct tcatgtacta caaactgctt ggctccaatg
     1981 gctaaagctc ttcaagacaa ctttggtgtt gttgaaggat tgatgactac tatccacgct
     2041 tacactggtg accaaatgat ccttgacgga ccacaccgtg gtggtgacct tcgccgtgct
     2101 cgcgctggtg ctgcaaacat cgttcctaac tcaactggtg ctgcaaaagc tatcggtctt
     2161 gtaatcccag aattgaatgg taaacttgat ggatctgcac aacgcgttcc aactccaact
     2221 ggatcagtta ctgaattggt agcagttctt gaaaagaacg ttactgttga tgaagtgaac
     2281 gcagctatga aagcagcttc aaacgaatca tacggttaca cagaagatcc aatcgtatct
     2341 tcagatatcg taggtatgtc ttacggttca ttgtttgacg caactcaaac taaagttctt
     2401 gacgttgacg gtaaacaatt ggttaaagtt gtatcatggt acgacaacga aatgtcatac
     2461 actgcacaac ttgttcgtac tcttgaatac ttcgcaaaga ttgctaaata attcttgagt
     2521 tgatagaaag caaggctttg tggtcttgct tttttatatg gaaaaatgga tgacacgatc
     2581 atccattctt ttttaattct ttttcaaatg tatttgaaag ggtagtgaaa gttagcctct
     2641 ctaaagtaag tgggtgggta aaggaaagtc ggaaggcatg aagcataagc cggcttgtct
     2701 ttgatttact attatagaga gggtctccca ggataggaag attatgatgc aaaaggtgca
     2761 cacgaatctg atgggttcgc cctgtcttta gcttgcaatg agccaaggaa gtcttgtttg
     2821 agaattgctt taatctgctt acatgcgttt cagcatattt cccatttttt gcatcaacta
     2881 ttctttttct acgatcatgg cgatcacgtc caattttgtc tctgaaaaca agttcttttc
     2941 tgttgatatt tccatcaact agagcccaat attctctaga aatctctttt ttctccaata
     3001 agcgattgag aatgggcagg ataaaaggat ttttggcaaa gagaactaag ccactggttt
     3061 ccatgtccag acgatgaacg acatagcagg tttggccaac ataggtactg acatggttaa
     3121 gaagggcaat ttcgtttggt tgattaccat gcgttttcat cccctctggt ttgtttacaa
     3181 taatcaagtg ttgatcttga taaacttcct gcactaagtc tgggttgccc caagggatcg
     3241 tcttttgggg ataatcttcc tcgtcaaaag tcaactggca aacatctcca ggatttacga
     3301 tttcgttcca gcggacttct tcttgattta tcaaaatatg tttcttgatt ctcaaaaaat
     3361 gacggatttt tctagggatg aggagttgtt cctcaagtaa ttgctttacc gtcatttgag
     3421 gtagagaggc gggtaatgta aatgtgaatt gcatacagat attgtaacaa aaaaagccct
     3481 atttggatag gaaatagcta aattcttgtc ttcctatgat gaagatgata aaataaacgc
     3541 atgaaattag ataaattatt tgagaaattt ctttctcttt ttaaaaaaga aacaagtgaa
     3601 ctagaggact ctgattctac tatcttacgt cgctctcgta gtgatcgaaa aaaattagcc
     3661 caagtaggtc cgattcgaaa attctggcgt cgttatcatc taacaaagat tatccttata
     3721 ctaggtttga gtgcaggctt gctagttgga atctatttgt ttgctgtagc caagtcgacc
     3781 aatgtcaatg atttgcaaaa tgccttgaaa actcggactc ttatttttga ccgtgaagaa
     3841 aaagaggctg gtgccttgtc tggtcaaaag ggaacctatg ttgagctgac tgacatcagt
     3901 aaaaacttgc agaatgctgt tattgcgaca gaagaccgtt ctttctataa aaatgacggg
     3961 attaactatg gccgtttctt cttggctatt gtcactgctg gacgttcagg tggtggctct
     4021 accattaccc aacagctggc taaaaacgcc tatttatcgc aggatcaaac tgttgagaga
     4081 aaagcgaaag aatttttcct tgccttagaa ttaagcaaaa aatatagtaa ggagcaaatt
     4141 ctaaccatgt accttaacaa cgcttatttt ggaaatggtg tgtggggtgt agaagatgcg
     4201 agtaagaaat actttggagt ttctgcatca gaagtgagtc tggatcaagc tgcgactctg
     4261 gcagggatgc tcaaggggcc ggaactgtat aatcccttga attccgtaga agattctact
     4321 aatcggcgcg atactgtctt gcagaatatg gttgcagcag gatatattga taaaaaccaa
     4381 gaaaccgaag ctgctgaagt tgatatgact tcgcaattgc acgataagta tgaaggaaaa
     4441 atctcagatt accgttaccc ctcttatttt gatgcggtgg ttaatgaagc tgtttccaag
     4501 tataatctaa cagaggaaga gattgtcaat aatggctacc gcatttacac agagctggac
     4561 caaaactacc aagcaaatat gcagattgtt tatgaaaaca catcgctatt tccgagggca
     4621 gaggatggaa cgtttgctca atcaggaagt gtagctctcg aaccgaaaac agggggagtt
     4681 cgtggagttg tcggtcaagt tgctgacaat gataaaactg gattccggaa tttcaactat
     4741 gcaacccaat caaagcgtag tcctggttct acaattaagc ctttagttgt ttatacacca
     4801 gcagttgaag caggctgggc tttgaataag cagttggata accataccat gcagtatgat
     4861 agctataagg ttgataacta tgcagggatc aaaacaagtc gagaagttcc tatgtatcaa
     4921 tccttggcag aatcgcttaa tctacctgct gttgccactg ttaatgattt gggtgttgac
     4981 aaggcttttg aggcaggcga aaaattcgga ctcaacatgg aaaaggtcga ccgtgttctt
     5041 ggtgtcgcct tgggaagcgg tgttgaaacc aaccctcttc aaatggctca agcatacgct
     5101 gcctttgcaa atgaaggttt aatgcctgaa gctcatttta ttagtagaat tgaaaatgct
     5161 agtggacaag ttattgcgag tcataaaaat tcacaaaaac gggtgattga taagtctgta
     5221 gctgacaaga tgaccagtat gatgttgggg actttcacca acggtaccgg tattagttca
     5281 tcgcctgcag actatgtcat ggcagggaaa actggaacaa ctgaagcagt tttcaatccg
     5341 gagtacacaa gtgaccagtg ggtaattggt tatactccgg atgtagtgat tagccactgg
     5401 cttggctttc cgaccactga tgaaaatcac tatctagctg gctctacttc aaacggtgca
     5461 gctcatgtct ttagaaacat tgccaatacc attttacctt atacgccagg aagtaccttt
     5521 acggttgaaa atgcttataa gcaaaatgga attgcaccag ccaatacaaa aagacaagta
     5581 caaaccaatg ataatagcca gacagatgat aatttgtctg atattcgagg gcgtgcgcaa
     5641 agtctagtag atgaggctag ccgggctatc tcagatgcga agattaagga aaaggctcaa
     5701 acaatatggg attcgatagt caatctattt cgctaagatg cttgtcaaag cctagctttc
     5761 ttgttataat ggataagatg gaggcgttat ggcactaaaa aaagcaagcc tagcttgtgc
     5821 ggtttgtggt tcgagaaact attcaatcaa gatcagcgga aaccccaagc ctacacgact
     5881 agaagtaaat aaattttgta agcattgtgg caagtacact acacacagag aaacgagata
     5941 ggagagagcg atgcgtttta ttggagatat ttttagactt cttaaagaca caacatggcc
     6001 aactcgcaag gaaagctgga gagattttcg ttctatcatg gaatacacag ctttctttgt
     6061 agtaattatt tacatttttg accagttgat tgtttcaggt ttgattcgat ttattaacat
     6121 tttttagaag attagtggag ttaattacac tagaaatctt ctatttatga aaggaaatat
     6181 catggatagt tttgataaag gatggtttgt tttacaaact tattctggtt atgaaaataa
     6241 ggtgaaagaa aatctattac aacgtgcaca aacctacaat atgttggata atattctacg
     6301 cgttgaaatt ccaacacaaa cagtgcaagt tgaaaaaaat ggaaagagaa aagaagtaga
     6361 agaaaatcgc tttccaggtt atgttcttgt agaaatggtc atgacagatg aagcttggtt
     6421 tgttgttcga aacacaccaa atgttacagg atttgtcgga tcacacggga acagatcaaa
     6481 accaactcca ttattggaac aagaaattcg cgacattttg gtatctatgg gacaaactgt
     6541 tcaagaattt gatttcgatg ttgagattgg tcaaactgta cgtattattg atggtgcttt
     6601 tgcagactac actggtaaga ttacagaaat tgataataac aaagtgaaaa tgattatctc
     6661 tatgtttggt aatgacacag ttgcagaagt aaatctaaac caaatcgcag aattataacc
     6721 ctgagagagg ctggagct
//
LOCUS       DAVDWM010000074         6457 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVDWM010000074 DAVDWM010000000
VERSION     DAVDWM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.074"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(108..404)
                     /locus_tag="VUW49_002036"
     CDS             complement(108..404)
                     /locus_tag="VUW49_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7981894.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(394..555)
                     /locus_tag="VUW49_002037"
     CDS             complement(394..555)
                     /locus_tag="VUW49_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET7981895.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            625..1389
                     /locus_tag="VUW49_002038"
     CDS             625..1389
                     /locus_tag="VUW49_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET7981896.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(1629..1781)
                     /locus_tag="VUW49_002039"
     CDS             complement(1629..1781)
                     /locus_tag="VUW49_002039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981897.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NISTAR"
     gene            1999..3378
                     /locus_tag="VUW49_002040"
     CDS             1999..3378
                     /locus_tag="VUW49_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET7981898.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVHSIFLTTFLVEGLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3392..4057
                     /locus_tag="VUW49_002041"
     CDS             3392..4057
                     /locus_tag="VUW49_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET7981899.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4367..4906
                     /locus_tag="VUW49_002042"
     CDS             4367..4906
                     /locus_tag="VUW49_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7981900.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            4973..5383
                     /locus_tag="VUW49_002043"
     CDS             4973..5383
                     /locus_tag="VUW49_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET7981901.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            5499..>6457
                     /gene="pavB"
                     /locus_tag="VUW49_002044"
     CDS             5499..>6457
                     /gene="pavB"
                     /locus_tag="VUW49_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET7981902.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYL"
BASE COUNT         1755 a         1235 c         1486 g         1981 t
ORIGIN      
        1 acagtgtttt gagcagcctg cggctaactt cctagtttgc actttaattt tcattgagta
       61 ttacttcatc ttctcatgaa attgagtttt tgcccagctc ctcttgatta ttctacttga
      121 ccgggccaag cattcatacc accttctacg ttggtaacgg tgaggccttg ggagctgaga
      181 aattggcagg tagatgcaga gcggactcct ccttgacaaa tgacatagta ttcatggtca
      241 ggtttgagtt ctttgtagcc ttgctctaag gtacttaatg gaagattttt ggcacctggt
      301 gcatgtcctg cttggaattc atgtgcttca cggacatcga taagttctag attttcattt
      361 tggtattttc cataaaagtc agccatgctg atactagttt ccatattcta ctccttctgg
      421 gttaaccatt ttgtatagtg aataagcgcc gtcaaggttt tgggcggtaa atcctgcttg
      481 cttgaggata ggctctgcga tatagctgcg caaaccactg tggcagctga cgatgtaggc
      541 ttgttttttg tccagttcat cagcttcctc cttgacactt tgtcagtttt tgatacaata
      601 gtacaaaatt agaggaggca ggctatgatt cagaaacatg cgattcctat tttagagttt
      661 gatgacaatc ctcaggcggt tatcatgccc aatcacgagg ggctggactt gcagttgcca
      721 aagaagtgtg tttatgcatt tttaggtgag gagattgacc gctatgcaag ggaagtaggg
      781 gcgaactgtg ttggcgaatt tgtttctgcc accaagacct atccagttta tgtcgtgaac
      841 tacaaggacg aggagatttg tctggttcag gctcctgtcg gttcagctcc agcagcccag
      901 tttatggatt ggttgattgg ctatggtgtg gagcagatta tctctactgg gacctgtggt
      961 gtcctagctg atatagagga aaatgccttt ctagtccctg ttcgcgctct gcgagatgaa
     1021 ggagccagtt accactatgt ggcaccttct cgttatatgg aaatgcagcc agaggctatt
     1081 gctgctattg aggaagtttt ggaagacaga gggattcctt atgaagaagt catgacctgg
     1141 acgacagacg gtttttaccg agaaacggct gaaaaggtgg cttatcgtaa ggaagaaggc
     1201 tgtgctgttg tggagatgga gtgttctgct cttgcggcag tagctcaatt gcgtggggtt
     1261 ctctggggtg aattgttgtt cacagcagat tctctagcgg acttggacca gtacgacagt
     1321 cgtgactggg gctcggaagc ttttaataag gcgctagaac tgagtttagc aagtgttcac
     1381 cacctttagt tgtactggca aaggatttgt tttatcataa aatgtctagc tcatactttt
     1441 caaaaatatg tttaaacgag gtcaccttcc tcttgtccta ggcatgttga ggttgggaaa
     1501 aatctttaaa atcagaaaaa cgtatcatat caggtgatga aaattttgat actatgcgtt
     1561 ttatgttggt aagatttaga gtgagatgaa atgatactct tcgaaaatct cttcaaacca
     1621 ggtcagcttc accttgccgt agatatgtta ctgacttcgt cagttctatc tgcaacctca
     1681 aaacagtgtt ttgagctgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag
     1741 caacctgtga ctagctttct aagcgatgcc ttggttctca ttgcctataa tcaaaaagag
     1801 aaattttctc ctgaaaagca tatagagtag ctggcgttaa aagctcctgt cttgcttttt
     1861 tgacctatag tcacatctat caagtattgt tcttgcctaa gctatcaata aaaaggtggc
     1921 attttttagg cttggtgtta gtagattttg ccttatccta tctaagtcat ttcgaacttt
     1981 ttatggtaca atggaaacat gttattcaaa ttatctaagg aaaaaataga gctaggctta
     2041 tctcgtttat cgccagcccg tcgtattttt ttgagttttg ccttggtcat tttactaggc
     2101 tctcttcttt tgagcttgcc ctttgtccaa gttgaaagct cacgagcgac ttattttgat
     2161 catcttttca ctgctgtctc tgcagtctgt gtgacaggtc tctcaaccct tccagtagct
     2221 cacacctata atatctgggg ccaaataatc tgtttgctct tgattcagat cggtggtcta
     2281 gggctcatga cctttattgg ggttttctat atccagagca agcaaaagct tagtcttcgt
     2341 agccgtgcaa ctattcagga tagttttagt tatggagaga ctcgatcttt gagaaagttt
     2401 gtccattcta tttttctcac aacttttcta gttgagggct tgggagcaat tttgcttagt
     2461 tttcgcctta ttcctcaact tggctgggga cgtggtcttt ttagttccat ttttctagcg
     2521 atctcagcct tctgtaatgc cggttttgat aatttaggga gcaccagttt atttgctttt
     2581 cagaccgatt tactggtcaa tctggttatt gcaggcttga ttattacagg cggccttggt
     2641 tttatggtct ggtttgattt ggctggtcat gtaggaagaa agaaaaaagg acgtctgcac
     2701 tttcatacga agcttgtact attattgact ataggtttgt tgttatttgg aacagcaact
     2761 actctctttc ttgagtggaa caatgctgga acgattggca atctccctgt tgccgataag
     2821 gttttagtta gcttttttca aacagtgacg atgcgaacag ctggcttttc tacgatagat
     2881 tatactcagg ctcatcctgt gactcttttg atttatatct tacagatgtt tctaggtggg
     2941 gcacctggag gaacagctgg gggactcaag attacgacat tttttgtcct cttggtcttt
     3001 gcacgaagtg agcttctagg cttgcctcat gccaatgttg cgagacgaac gatcgcgccg
     3061 cgaacggttc aaaaatcctt tagtgtcttt attatctttt tgatgagctt cttgatagga
     3121 ttgattctgc tagggataac agccaaaggc aatcctccct ttatccacct cgtatttgaa
     3181 accatttcag ctcttagtac agttggtgta acggcaaatc tgactcctga ccttgggaaa
     3241 ttggctctca gtgttatcat gccacttatg tttatgggac gaattggtcc cttgaccttg
     3301 tttgttagct tggcagatta ccatccagaa aagaaagata tgattcacta tatgaaagca
     3361 gatattagta ttggttaaga aaggaaagag catgtcagat cgtacgattg gaattttggg
     3421 cttgggaatt tttgggagca gtgtcctagc tgccctagcc aagcaggata tgaatattat
     3481 cgctattgat gaccacgcag agcgcatcaa tcagtttgag ccagttttgg cgcgtggagt
     3541 gattggtgac atcacagatg aagaattatt gagatcagca gggattgata cctgcgatac
     3601 cgttgtagtc gcgacaggtg aaaatctgga gtcgagtgtg cttgcggtta tgcactgtaa
     3661 gagtttgggg gtaccgactg ttattgctaa ggtcaaaagt cagaccgcta agaaagtgct
     3721 agaaaagatt ggagctgact cggttatctc gccagagtat gaaatggggc agtctctagc
     3781 acagaccatt cttttccata atagtgttga tgtctttcag ttggataaaa atgtgtctat
     3841 cgtggagatg aaaattcctc agtcttgggc aggtcaaagt ctgagtaaat tagacctccg
     3901 tggcaaatac aatctgaata ttttgggttt ccgagagcag gaaaattccc cattggatgt
     3961 tgaatttgga ccagatgacc tcttgaaagc agatacctat attttggcag tcatcaacaa
     4021 ccagtatttg gataccctag tagcattgaa ttcgtaaaga gggatgaccc ctcttttttg
     4081 atgcccaaga tggcaaatag agacagaagc cccttgtctt ctagtaaaag ttcttcaaag
     4141 gctggacttt atggtaaaat agaaggaagt gacaagagag agtaatactc aatgaaaatc
     4201 aaagatcaaa ctaggaaact agctgcgggc tgctcaaaac actgttttga ggttgcagat
     4261 agaactgacg aagtcagtaa catctatacg gcaaggcgac gttgacgcgg tttgaagaga
     4321 ttttcgaaga gtataagaaa aaatcagtcc cctaaaggag cagattatga agttattgtc
     4381 tatcgccatt tctagctata atgcagcagc ctatcttcat tactgtgtgg agtcgctagt
     4441 gattggtggt gagcaagttg ggattttgat tatcaatgac gggtctcagg atcagactca
     4501 ggaaatcgct gagtgtttag ctagcaagta tcctaatatc gttagagcca tctatcagga
     4561 aaataaaggc catggcggtg cggtcaatcg tggcttggca gaggcttctg ggcgctattt
     4621 taaagtagtt gacagtgatg actgggtgga tcctcgtgcc tacttgaaaa ttcttgaaac
     4681 cttgcaggaa cttgagagca aaggtcaaga ggtggatgtc tttgtgacca attttgtcta
     4741 tgaaaaggaa gggcagtctc gtaagaagag tatgagttac gattcagtct tgcctgttcg
     4801 gcagattttt ggctgggacc aggtcggaaa tttctccaaa ggccagtata ccatgatgca
     4861 ctcgctgatt tatcggacag atttgttgcg tgctagccag ttctaactgc ctgaacatac
     4921 tttttatgtc gataatctct ttgtctttac gccccttcag caggtcaaga ccatgtacta
     4981 tctgcctgtc gatttctatc gttatttgat tgggcgtgag gaccagtctg tcaatgagca
     5041 agtgatgatt aagtgcattg accagcaact caaggtcaat cgactcttga tagaccaact
     5101 tgatttgtcc caagtgagtc atcctaaaat gcgagaatat ctgctgaatc atattgaaat
     5161 cacgacggtg atttccagta ccctgctcaa ccgatctgaa acagcggagc atctggcaaa
     5221 aaaacgccaa ttgtggacct atattcagca ggaaaatcca aaagtctttc aggctattcg
     5281 taagaccatg ttgagccgtt tgaccaaaca ttctgtcttg ccagatcgca aactgtccaa
     5341 tgtcgtctat caaatcacca aatctgttta tggatttaat taatataagt gttttataag
     5401 agggatttaa gaaaaatttt aactttttct tagtcctttt taattttagg agattatact
     5461 agagtcatca aataaagaaa gactctaagg agaatcctat gaaattcaat ccaaatcaaa
     5521 gatatactcg ttggtctatt cgccgtctca gtgtcggtgt tgcctcagtt gttgtggcta
     5581 gtggcttctt tgtcctagtt ggtcagccaa gttctgtacg tgccgatggg ctcaatccga
     5641 ccccaggtca agtcttacct gaagagacat cgggaacgaa agagggtgac ttatcagaaa
     5701 aaccaggaga caccgttctc actcaagcga aacctgaggg cgttactgga aatacgaatt
     5761 cacttccgac acctacagaa agaactgaag tgagcgagga aacaaactct tctagtctgg
     5821 atacactttt tgaaaaagat gaagaagctc aagaaaatcc agagctaaca gatgccttaa
     5881 aagaaactgt agatacagct gatgtggatg ggacacaagc aagtccagca gaaactactc
     5941 ctgaacaagt aaaaggtgga gtgaaagaaa atacaaaaga cagcatcgat gttcctgctg
     6001 cttatcttga aaaagctgaa gggaaaggtc ctttcactgc cggtgtaaac caagtaattc
     6061 cttatgaact attcgctggt gatggtatgt taactcgtct attactaaaa gcttcggata
     6121 atgctccttg gtctgacaat ggtactgcta aaaatcctgc tttacctcct cttgaaggat
     6181 taacaaaagg gaaatacttc tatgaagtag acttaaatgg caatactgtt ggtaaacaag
     6241 gtcaagcttt aattgatcaa cttcgcgcta atggtactca aacttataaa gctactgtta
     6301 aagtttacgg aaataaagac ggtaaagctg acttgactaa tctagttgct actaaaaatg
     6361 tagacatcaa catcaatgga ttagttgcta aagaaactgt cgaaaaagct gttaaagata
     6421 acgttaaaga cagtatcgat gttccagcag cctacct
//
LOCUS       DAVDWM010000075         5852 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVDWM010000075 DAVDWM010000000
VERSION     DAVDWM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.075"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1..334
                     /locus_tag="VUW49_002045"
                     /pseudo
     CDS             1..334
                     /locus_tag="VUW49_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            489..689
                     /locus_tag="VUW49_002046"
     CDS             489..689
                     /locus_tag="VUW49_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981903.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKAFATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            714..1130
                     /locus_tag="VUW49_002047"
     CDS             714..1130
                     /locus_tag="VUW49_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981904.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1361..2068
                     /gene="ccdA2"
                     /locus_tag="VUW49_002048"
     CDS             1361..2068
                     /gene="ccdA2"
                     /locus_tag="VUW49_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET7981905.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2079..2636
                     /gene="sdbB"
                     /locus_tag="VUW49_002049"
     CDS             2079..2636
                     /gene="sdbB"
                     /locus_tag="VUW49_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET7981906.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIVPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3010..4401
                     /locus_tag="VUW49_002050"
     CDS             3010..4401
                     /locus_tag="VUW49_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7981907.1"
                     /translation="MNIFRTKNVSLDKTEMHRYLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4547..5464
                     /gene="adcAII"
                     /locus_tag="VUW49_002051"
     CDS             4547..5464
                     /gene="adcAII"
                     /locus_tag="VUW49_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET7981908.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5472..>5852
                     /locus_tag="VUW49_002052"
     CDS             5472..>5852
                     /locus_tag="VUW49_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET7981909.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYV"
BASE COUNT         1788 a         1037 c         1236 g         1791 t
ORIGIN      
        1 ctactatgaa caagagccag aaaaagtagc cttatttctt aagaatttta atagtttaaa
       61 gcacctagca cctgtttaga ttgacgaaac aggatttgat acttattttt atcgagaata
      121 tggtcgctca ttaaaaggtc agttaataag aggcaaagta tctggaagaa gatatcagag
      181 gatttctttg gttgcaggtc taaccaatgg tgagttaatc gctccaatga cttacgaaga
      241 gacgatgacg agcgactttt ttgaagctta gtttcagaaa tttctcttac caacattaac
      301 cacaccatcg gttattatat taaaacgaaa taagaatagg acaaatcgat caggacagta
      361 aaatcgaatt ctaacaatgt tttagaagtc catatgtact attctagttt caatatacca
      421 taactgctcc tagtcaacct gcaagtcctg tcccagctcc aagctcagct ccgactcaag
      481 atcctagagt ggtagtaagc gcttcggcta gctcaacgag cccacaagct caagagcaag
      541 ttgacaagtc tgaacttcgt gccttgagtc aagagttaga ccaacacttg aaagcttttg
      601 caacagtatc tgatccgaaa atcgatgcaa ccaaggctgt cctcctagat gctcaaaaag
      661 ctccggaaga tagtgccttg acagagtaag gtctgcatac tgcagtagag tctgtgaagg
      721 ctgccttgga ctctctcaaa gatgtgaaag caaatgctag cgacagcaag cctgcacagg
      781 acaagaagga tgcaaaacaa ggaacggaag atagtaagga ttcagataag atgactgaaa
      841 caaactcagt tccggcagga gtgattgtgg tcagtctact tgccctccta ggcgtgattg
      901 ccttctggct gattcgccgt aagaaagagt cagaaatcca gcaattaagc acggaattaa
      961 tcaaggttct aggacagcta gatgcagaaa aagcggataa aaaagtcctt gccaaagccc
     1021 aaaaccttct ccaagaaacc cttgatttcg tgaaagaaga aaatggctca gcagagacag
     1081 aaactaaact agtagaggag cttaaagcaa tccttgacaa actcaagtaa aacatgattt
     1141 atgaaatgtc agacttaaaa gacattttcg ttcacaaaag cagcgacgag taatccttgt
     1201 cgttgctttt tggctatttt gggggataag atccgccttg actagcaaaa gaaaagattg
     1261 gtccaatagt gcccgatacg attttgcgta gcagaaagct tacaaataaa attgaatata
     1321 gtaaaatagg attagaatta ttaagaaagt tggttcttta ttggaaacga tagtattttt
     1381 aatctctgtt tttctagcag gtgttttatc ctttttttct ccttgtattt ttcctcttct
     1441 gccagtctat gctgggattt tattggatga tcaagaaagt gcaaaaagct tttctttgtt
     1501 tgggagaaag gttctctggt caggcttgat tcgaacactt tgctttatcg ctggtatctc
     1561 tctcattttc tttattctag gctttggtgc tggttacttt ggtcatattc tctatgcaaa
     1621 ttggtttcga tatggcatgg gagctattat tatcattttg ggtcttcacc agatggaaat
     1681 ttttcatttg aagaaattag aagttcaaaa aagttttacc tttaaaaaat cagattctaa
     1741 tcgttattgg tcagcttttt tacttggtat tacctttagc tttggttgga caccttgtat
     1801 tggtccagtt ttaagttctg ttttagcact tgcggcttct ggaggcaatg gcgcttggca
     1861 aggagcgatt tatactctca tttacactct gggcatggcc cttcctttct tggtattggc
     1921 actagcttca ggtctagtca tgccatattt tagtaaaatc aagcgtcata tgatgctact
     1981 aaagaaaatt ggtggtttcc tcattgtttt aatgggaatt ttgttactat taggacaagt
     2041 aaatgttcta gctggaattt ttgaataaag gaggacaaat gaaaaaagta atgtttgctg
     2101 gcttaagtct cttgtcatta gttgtattga tggcctgtgg tgaggaagaa actaaaaaga
     2161 ctcaagcagc acaacagcca aaacaacaaa cgactgtaca acaaattgct gttggaaaag
     2221 atgctccaga cttcacattg caatccatgg atggcaaaga agttaagtta tctgatttta
     2281 agggtaaaaa ggtttacttg aagttttggg cttcatggtg tggtccatgc aagaaaagta
     2341 tgccagagtt gatggaacta gcggcgaaac cagatcgtga tttcgaaatt cttactgtca
     2401 ttgtaccagg aattcaaggt gaaaaaactg ttgagcaatt cccacaatgg ttccaggaac
     2461 aaggatataa ggatatccca gttctttatg ataccaaagc aaccaccttc caagcttatc
     2521 aaattcgaag cattcctaca gaatatttaa ttgatagcca aggaaagatt ggaaagattc
     2581 aatttggtgc tatcagtaat gcggatgcag aagcagcatt taaagaaatg aactagaatc
     2641 gataaaatct gattacaaga tgtagtcaga ttttggtaac ttctaaaact aacttccagt
     2701 ttcccacaac ctagctttta gtatgagaaa aacgcgtgac atcagtggaa atccgtctta
     2761 gactaacttc cactggtttt ctttttcttg atatataagg gttcaaaagc gaaaagactc
     2821 agaaaaagtg cacgacaaat agccctaaaa cagagtcgtc ttagagtaac ttccagtttt
     2881 gggagagaga tggaagttac tttgagaagt tacggtagtc agattttttt agaaaaacat
     2941 tttataaata ttcacatatc tcctatgttt atggtaaaat agaattatca gtttattttg
     3001 gagtcaaaga tgaatatatt tagaacaaag aatgttagtt tagataaaac agagatgcat
     3061 aggtatttga agttatggga tttgattttg ctgggtatcg gagccatggt agggacaggc
     3121 gtctttacaa tcacaggtac tgcagctgca acacttgctg gcccagccct agtgatttca
     3181 atcgttattt ctgccttgtg tgtgggatta tcagccctct tttttgcaga atttgcctcg
     3241 cgagtacccg ctacaggagg tgcctatagt tacctctatg ctatcttagg agaattccct
     3301 gcctggttgg ctggttggtt aaccatgatg gagttcatga cagccatatc aggcgtagct
     3361 tcgggttggg cagcttattt taaaggcttg ctctctcaat acgggatagc ctttcctcag
     3421 gctttaaatg gtacctttaa tcctcaagca gggacatttg ttgatttatt gcctattctt
     3481 gtcttggtct tggtgacttc gcttgtttta ctaaatgcca aggcagcctt acgctttaat
     3541 tcgattctag ttattttgaa attttccgct ttagctctct ttgttttagt aggaatttgg
     3601 aatattaagt ttgataattg gagcaatttt gctccttacg gttttggaca aatctatgga
     3661 gctagcactg gtattatggc tggtgcgtcc ttgatgttct tcggtttttt gggatttgaa
     3721 tccatctcta tggctgtaga tgaagtcaag actcctcaaa aaaatattcc tagagggatt
     3781 gtcttatcgc tttctatcgt aaccattctc tatgccttgg tgacccttgt tttgactggt
     3841 gtggttcact atagtcattt aaatgtcgat gatgccgttg cctttgccct tcgtagtgtt
     3901 gggattagtt gggcagccaa ctatgtgtca ttagtggcta tcttgacctt gattacagtt
     3961 tgtatttcga tgacctatgc cctatcgcgt atgatttaca gtttagcacg tgacggctta
     4021 gtgcctgctg cctttaagga actgacgaag actagcaaga taccaaagaa tgctactatc
     4081 ttgacaggtc tagcttcagc agtagcagca ggaatgttcc cactagccag tattgcagcc
     4141 tttttaaata tttgtacctt agcctacttg atcatgttgg cttatggtct gattcgctta
     4201 cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa caccattggt acctctgtta
     4261 ccaattttat cgatcatcat ttgtttatcc ttcatgttac agtataatat gaatacctgg
     4321 attgctttcc tagttgcgtt gttagtagga agtattattt acttcactta tggctataag
     4381 cattctacca tagaagaata aagtgaaagt ctattgagtt gttgacactc agcagatttt
     4441 tatatgtgaa agaaatttca aattttttct aaaaatatct tgacagatta aattttcagg
     4501 agtagaatat ttactagtta attaaatggt taaggagttg ttcatcatga agaaacaaaa
     4561 tttattttta gtcctgttaa gtgtctttct tttgtgcttg ggggcttgtg gtcaaaagga
     4621 aagtcagaca ggaaagggga tgaaaattgt gaccagtttt tatcctatct acgctatggt
     4681 taaggaagta tctggtgact tgaatgatgt tcggatgatt cagtcaagta gtggtattca
     4741 ctcctttgaa ccttcggcaa atgatatcgc agccatctat gatgcagatg tctttgttta
     4801 ccattctcat acactcgaat cttgggcagg aagtctggat ccaaatctaa aaaaatccaa
     4861 agtgaaggtc ttagaggctt ctgagggaat gaccttggaa cgtgtccctg gactagagga
     4921 tgtggaagca ggggatggag ttgatgaaaa aacgctctat gaccctcaca catggctaga
     4981 tcctgaaaaa gctggagaag aagcccaaat tatcgctgat aaactttcag aggtggatag
     5041 tgagcataaa gagacttatc aaaaaaatgc gcaagccttt atcaaaaaag ctcaggaatt
     5101 gactaagaaa ttccaaccaa aatttgaaaa agcgactcag aaaacatttg taacacaaca
     5161 tacagccttt tcttatctag cgaagagatt tgggcttaat caacttggta ttgcaggtat
     5221 ctctcctgaa caagaaccaa gtccacgaca actaacagaa attcaggaat ttgttaagac
     5281 ctataaggtt aaaacgattt ttacagaaag taacgcttct tcaaaagtag ctgaaactct
     5341 tgtcaaatca acaggtgtgg gtcttaaaac tctgaatcct ttagagtcag acccacaaaa
     5401 tgacaagacc tatttagaaa atcttgaaga aaatatgagt attctagcag aagaattaaa
     5461 gtgaggaaag aatgaaaatc aataaaaaat atctagcagg ttcagtggca gtccttgccc
     5521 taagtgtttg ttcctatgaa cttggtcgtc accaagctgg tcaggttaag aaagagtcta
     5581 atcgagtttc ttatatagat ggtgatcagg ctggtcaaaa ggcagaaaat ttgacaccag
     5641 atgaagtcag taagagagag gggatcaacg ccgaacaaat cgtcatcaag attacggatc
     5701 aaggttatgt gacctctcat ggagaccatt atcattacta taatggcaag gtcccttatg
     5761 atgccatcat cagtgaagag ctcctcatga aagatccgaa ttatcagttg aaggattcag
     5821 acattgtcaa tgaaatcaag ggtggttatg tc
//
LOCUS       DAVDWM010000076         5452 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVDWM010000076 DAVDWM010000000
VERSION     DAVDWM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.076"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1122..2381
                     /locus_tag="VUW49_002053"
     CDS             1122..2381
                     /locus_tag="VUW49_002053"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7981910.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2397..2948
                     /locus_tag="VUW49_002054"
     CDS             2397..2948
                     /locus_tag="VUW49_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981911.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2941..4218
                     /locus_tag="VUW49_002055"
     CDS             2941..4218
                     /locus_tag="VUW49_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7981912.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4234..5094
                     /locus_tag="VUW49_002056"
     CDS             4234..5094
                     /locus_tag="VUW49_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET7981913.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         1659 a          886 c         1229 g         1678 t
ORIGIN      
        1 tcatatggga ctttttttct acacaaaaat agactccata atatctataa gggatttacc
       61 cactacaaat attatagagc caaaaatccc gtgcctcatc agacacggga ttttgtggta
      121 cgacaggcat gtatagcaaa ctgaatctgg aatagcacag catatcttct aaaatattgt
      181 tataaattga tttgaatttc aaaattaaat tgtttgattc ttatttcaat ttgttatagt
      241 atatctgatg tcaaagttct cggcgagtca aatagcgatt cccaagcctg actatcgtga
      301 ggtagcggat taaaatggtc tggggataga ccgttttaag tctgacgctg gaaataagaa
      361 ttgtcagaag aagggatagc gaaatcgtgg ctctacgaac aggaacgtga taataaggcg
      421 tatatagcgg ataagagggc ctcaaactct aaagtccaaa aaggtagtcg taacctatat
      481 gcgtaaatca cgagagtaat tgaattcgta ctaagatttt ctattttcac tgtaaccttt
      541 taatgtcctt atatcttgta tacacgagga aagatgtacg atttatcccg tgaggtctat
      601 cactataaag agaaaacgac agatagaagt gatcctgagt cacggttatc tttaaaatag
      661 attgtctgat aggacggtat gtataaaacg cttctgtgaa ctgagagaag gggagaagtt
      721 cttgctaaaa tttagttgaa tagccgtatt ccgatactta gataagagat ctagtcttag
      781 ctcctactca gttttagggg ataaaaaagg ggcaatagcg attcgagaaa gattatactc
      841 ttcgaaaatc tcttcaaatc acgtcaatat cgccttgccg tatatgtagg atactgacta
      901 cgtcagttcc atctacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag
      961 tttgatcttt gattttcatt gagtattagt aattcagtta ctaactcgtc aactctgatt
     1021 tatccaataa aattgaaaag gatggaaaaa aggataaatt tatgatatac tttattttga
     1081 agaccttatt agaaatcttg aaagagtatt gaaaacttag aatgagaaaa attgttatca
     1141 atggtggatt accactgcaa ggtgaaatta ctattagtgg tgctaaaaat agtgttgtgg
     1201 ccttaattcc agctattatc ttggctgatg atgtggtgac tttggattgt gttccagata
     1261 tttcggatgt agccagtctt gtcgaaatca tggaattgat gggagctact gttaagcgtt
     1321 atgacgatgt cttggagatt gatccaagag gtgttcaaaa tattccaatg ccttatggta
     1381 aaattaacag tcttcgtgca tcttactatt tttatgggag cctcttaggc cgttttggtg
     1441 aagcgacagt tggtctaccg ggaggatgtg atcttggtcc tcgtccgatt gacttacacc
     1501 ttaaggcgtt tgaagctatg ggtgccactg ctagctacga gggagataac atgaagttat
     1561 ctgctaaaga tacaggactt catggtgcaa gtatttacat ggatacggtt agtgtgggag
     1621 caacgattaa tacgatgatt gctgcggtta aagcaaatgg tcgtactatt attgaaaatg
     1681 cagcccgtga acctgagatt attgatgtag ctactctctt gaataatatg ggtgcccata
     1741 tccgtggggc aggaactaat atcatcatta ttgatggtgt tgaaagatta catgggacac
     1801 gtcatcaggt gattccagac cgcattgaag ctggaacata tatatcttta gctgctgcag
     1861 ttggtaaagg aattcgtata aataatgttc tttacgaaca cctggaaggg tttattgcta
     1921 agttggaaga aatgggagtg agaatgactg tatctgaaga cagcattttt gtcgaggaac
     1981 agtctaattt gaaagcaatc aatattaaga cagctcctta cccaggcttt gcaactgatt
     2041 tgcaacaacc gcttacccct cttttactaa gagcgaatgg tcgtggtaca attgtcgata
     2101 cgatttacga aaaacgtgta aatcatgttt ttgaactagc aaagatggat gcggatattt
     2161 cgacaacaaa tggtcatatt ttgtacacgg gtggacgtga tttacgtggg gccagtgtta
     2221 aagcgaccga cttaagagct ggggctgcac tagtcattgc tgggcttatg gctgaaggta
     2281 aaactgaaat taccaatatc gagtttatct tacgtggtta ttctgatatt atcgaaaaat
     2341 tacgtaattt aggagcggat attagacttg ttgaggatta aaccgtagag gtgtttatga
     2401 atatttggac caaattagca atgttttctt tttttgaaac ggatcgcttg tatttgcgtc
     2461 ctttcttttt tagtgatagt caggacttcc gcgagatagc ttcaaatcca gaaaatcttc
     2521 aatttatttt cccaacgcag gcaagtctgg aagaaagtca atatgcactg gccaattact
     2581 ttatgaagtc ccctttggga gtgtgggcaa tttgtgacca gaaaaatcaa caaatgattg
     2641 gttctattaa atttgagaag ttagatgaaa tcaaaaaaga agctgagctt ggctattttt
     2701 tgagaaaaga tgcttggtcg caaggattta tgacagaggt tgttagaaaa atttgtcagc
     2761 tttcttttga ggaatttggc ttaaaacaat tatctatcat tacccacctt gaaaatgaag
     2821 ctagccaaag agttgctctt aagtctggat ttagtttgtt ccgtcagttt aagggaagtg
     2881 atcgttacac aagaaaaatg cgggattatc ttgaatttcg gtatgtaaaa ggagagttca
     2941 atgagtaagc atcaggaaat tctaagctat ttggaggaat taccagtagg taaaagagtc
     3001 agtgttcgta gcatttcgaa tcatctagga gttagtgatg gaacagccta tcgggctatt
     3061 aaagaagctg aaaaccgtgg aattgtggag acccgtccta gaagtggaac aattcgtgtt
     3121 aaatcccaga aagttgctat agagagatta acgtttgctg aaattgcaga agtgacttct
     3181 tctgaggttc tggctgggca agaaggttta gagagagaat ttagtaagtt ttcaattggt
     3241 gccatgactg aacaaaatat cttgtcttac cttcatgatg gggggctctt gattgtcgga
     3301 gaccgaaccc gtattcagtt gctagccttg gaaaatgaaa atgcagttct ggttacaggg
     3361 ggatttcagg ttcatgatga tgtgcttaaa ctggccaatc aaaaagggat tcctgttcta
     3421 agaagtaagc atgatacctt taccgtcgcg accatgatca ataaagcctt gtcaaatgtc
     3481 caaatcaaga ctgatattct gacagttgag aaactttatc gccctagtca tgagtatggt
     3541 tttctgagag agacagatac agttaaagat tatttggact tggttcgtaa gaatcgtagc
     3601 agccgtttcc ctgttatcaa tcaacatcag gtcgttgttg gtgttgtaac catgagagac
     3661 gctggtgata aatcaccaag cacgacaatt gataaggtta tgtctcgtag tctatttttg
     3721 gttggattat cgacaaatat tgccaatgtg agtcaacgga tgatcgcaga agactttgaa
     3781 atggtaccag ttgttcgaag caatcaaact ttgcttggcg ttgtgacgcg acgagatgtc
     3841 atggagaaga tgagccgttc ccaagtttcg gctctaccaa ctttttctga gcagattgga
     3901 caaaagctct cttatcacca tgatgaagta gtcattacag tggaaccctt tatgctagaa
     3961 aaaaatggag ttttggctaa tggtgtattg gcagaaattc tgacccacat gacccaagat
     4021 ttagttgtta atagtggtcg caatctcatt atcgagcaga tgctgatcta ctttttgcag
     4081 gctgttcaga tagatgatat attgcgcatt caggcacgga ttattcatca tacgagacgg
     4141 tcagctataa ttgattacga tatttatcat ggtcaccaga ttgtttcaaa agcaaatgtg
     4201 actgttaaaa ttaattagaa actaggagaa aagatgataa cattaaaatc agctcgtgaa
     4261 atcgaagcta tggacaaggc tggtgatttt ctagcaagta ttcatatagg cttacgtgat
     4321 ttgattaaac caggcgtaga tatgtgggaa gttgaagaat atgtccgccg tcgttgtaaa
     4381 gaagaaaatt tccttccact tcagattggg gttgacggtg ccatgatgga ctatccttat
     4441 gctacctgtt gctctcttaa cgatgaagtg gctcacgctt tccctcgtca ttatatcttg
     4501 aaagatggtg atttgctcaa agttgatatg gttttgggag gtcccattgc taaatctgac
     4561 ctgaatgtct caaaattaaa cttcaacaat gttgaacaaa tgaaaaaata cactcagagc
     4621 tattctggtg gtttagcaga ctcatgttgg gcttatgctg ttggtacacc gtccgaagaa
     4681 gtcaaaaact tgatggatgt aaccaaagaa gctatgtaca agggtattga gcaagctgtt
     4741 gttggaaatc gtatcggtga tatcggtgcg gctattcaag aatacgctga aagtcgtggt
     4801 tacggtgtag tgcgtgattt ggttggtcat ggtgttggcc caactatgca cgaagaacca
     4861 atggttccta actatggtat tgcaggtcgt ggactccgtc ttcgtgaagg aatggtctta
     4921 accattgaac caatgatcaa tacaggcgat tgggaaattg atacagatat gaaaactggt
     4981 tgggcgcata agaccattga cggtggattg tcatgtcagt atgaacacca atttgtcatt
     5041 acgaaagatg gacctgttat cttgactagc caaggtgaag aaggaactta ttaataaaaa
     5101 gtgaaaagac tactggaagt ttattttgat aaaaaatcca gtagatcttt tcataataaa
     5161 acgcattgta tcaagtgtta ggggctgata tcatgcgttt ttctgttttt aagatttttt
     5221 ccaactctgt ttgtaagcgc atcataacaa agggtctagg attcagggct ctcctcctat
     5281 atactattag taaagtaaaa ctaagggagg atattttagt gtcgcagtct attgttcctg
     5341 tagagattcc acaatattgt cgttttgatt ctaaaaagag aaatggaatt ctatttaacg
     5401 ttcgtattgc caatcttaaa tttacctttt ttagatcaga cttcctgcga aa
//
LOCUS       DAVDWM010000077         5325 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVDWM010000077 DAVDWM010000000
VERSION     DAVDWM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.077"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            520..2853
                     /locus_tag="VUW49_002057"
     CDS             520..2853
                     /locus_tag="VUW49_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET7981914.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2866..4329
                     /locus_tag="VUW49_002058"
     CDS             2866..4329
                     /locus_tag="VUW49_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET7981915.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDPHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4329..>5325
                     /locus_tag="VUW49_002059"
     CDS             4329..>5325
                     /locus_tag="VUW49_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7981916.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFAYGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLAEQQRIIEAIESALEKVDEYAESYNRLEQLDKKFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDD"
BASE COUNT         1904 a          791 c         1069 g         1561 t
ORIGIN      
        1 aaatctgaat cgcaaaaact atcaataaaa cccaatctat tatgaaagtc aaaaacactt
       61 cccaactgaa agaactacct ccagtgacaa actttgagaa aaacggtagt agagctaaaa
      121 agagaagcaa aataggaagc atccgcattg ttaaaatcct tttggcataa aaaaatcttt
      181 atttaaacga aaatattatg gcaaaattta cgccagtttt tgaacggctg atgtagatat
      241 tttatacttt caaaatgttt aaatgtgatt atttattttt gaaaaatagc tcaccagccc
      301 gactgaaagt gcttataaat gataataagt cgcctgccga aaacagcgaa aaatagcggt
      361 gttatgcgga gataatctga cgcgatgcga aagtatattg catacttatt ttcaacaatt
      421 tagcagagta tttttataag tgtgatataa tagaagtata atttgttctg atagtttatt
      481 ttatggagaa gtagattttt agaatgcgga gggttcaata tggttgagtt tataaagtct
      541 aagaaagaaa tgagtgagga ggatattaaa gcaaatttca tcactcctgc tattgtatcc
      601 aaaggatgga aaaatggtga gcatatcgct tacgaagaat acttcactga tggtcgaatt
      661 gaggttagag gagataaggc tcgtcgtaaa gaaggaaaaa aatcagacta ttcactgtat
      721 taccaatttg gaactcgaat tgcaattgtt gaggcaaagg ataataaaca cagcgttcga
      781 gcaggattac aacaagctat tgaatatgga gagattttag atgttccatt tgtttattct
      841 tcgaatggtg atggctttat tgaacacgac cgtatcacga gagaagaacg tgagctggag
      901 ttagacgaat tccctactcg tgaagaatta ttttctcgta tgacgaagga aaaaggattg
      961 acgtacgaaa ttacagaagc tatctcaact ccatactata cagacgcctt ctcaatgaaa
     1021 acgccacgct attatcagca aatagctatc aaccgtacta ttgaaacagt tgccagagga
     1081 caaaaacgag taatgtttgt gatggcaaca ggaacgggga aaacgttcat ggcttttcaa
     1141 attattcatc gccttcgaaa agctggtttg gctaaacgag ttttattctt agcggataga
     1201 aacatcttag tagaccaaac gatggctgaa gactttaggc cattcgaaaa ggtaatgacg
     1261 aaaattacac caaaactttt gactgctcct gaaaaattaa attcttttga aatttatcta
     1321 gggctttatc agcaactaac tggtgaagat ggaactgaaa cacattatca aaaatttgac
     1381 aaagacttct ttgatttaat cgtaattgat gaagcgcacc gtggttcagc taaggaaaac
     1441 agtaactggc gtaagataat tgattatttc agttctgcga cacagattgg gatgaccgct
     1501 actcctaaag aaaccaagaa tgcttccaat acggaatact ttggtgagcc aatctatact
     1561 tatagtttaa aacagggaat cgaggatggt tttttggctc catatcgtgt tatgagagtt
     1621 aatttagatg tagatgtgga tggttatcgt ccagaaactg gaaaagttga tgctaacgga
     1681 caattaatag aagataggta ctacggcagg aaagattttg ataaaaccat tgtcattgat
     1741 gatagaacgc aaagagttgc caagtttgtt tctgattata tgaagcaaaa caatgcacga
     1801 tttgataaaa caattgtttt ttgtgttgat attgaccatg ccgagcgaat gcgtgctgca
     1861 cttgtaaaag agaatctaga cttagtccaa gaagactatc gttatgtcat gcaagtaact
     1921 ggtgacaacg ctgaaggaaa agctcaactg gataacttta tggatgtcaa ttctaatttt
     1981 cccgctattg taacaacgtc taaattatta acgacaggag ttaatgctaa aacatgtcgt
     2041 ttgattgttt tagactctaa tatccaatcc atgactgaat ttaaacaaat tattggtcgt
     2101 ggcacacgtc tttatcctca aaaggggaaa gaatttttta cgattattga ttttcgaaat
     2161 gttaccaatt tgtttgctga ccctgatttt gatggtgatc cagtgaaggt gctggaaaca
     2221 ggtgcgaaaa cagtcagtgg ttctacgccc ggtttcgtaa gtgaggaagg tgacccagta
     2281 gaaaaatata tcgttacaga caagcaggtt accattctta attctactgt tcaagtattg
     2341 gatgaaaacg ggaaactgat taccgaaagc ctgaccgact acactcggaa gaatatctta
     2401 ggtagctacg ccactttgaa cgattttatc acagtttggc atacggcgga taaaaagaag
     2461 cttatcttag acgaacttta taaaaaagga gtttatctag atgctattcg agagtcggag
     2521 ggaatatcag aacaagaaat cgatgatttt gatttactcc taaaacttgc ctatggtcaa
     2581 aaagaattaa ccaaaacgga acgtatcaat aagctcaaac aaagcggata tttatataaa
     2641 tatagtgagg aagcgcgtgc tgttttggaa attttactga acaaatacat ggataaaggt
     2701 attggagaac tcgaaagcat tgaaacatta aaacttccag aatttcagat atatggtgga
     2761 accttcaaaa tcatcaatac ttattttgga gataaaaaac gatatttaca agcaattaaa
     2821 gaattggagc aagagctatt tacagtagct taatgaaagg aaagtatgtc aattacatca
     2881 tttgtaaaaa gaattcaaga tatcactcga aacgatgctg gtgttaatgg tgatgctcaa
     2941 cgtattgagc aaatgtcttg gttattattc ttaaaaattt atgatagccg tgaaatggtt
     3001 tgggaattag aagaagacga gtatgagtca attatcccag aggaattaaa atggcgaaat
     3061 tgggctcatg ctcaaaatgg ggaacgggta ttgacaggcg atgaattact tgattttgtc
     3121 aataacaagt tattcaaaga gttgaaagag cttgaaataa cttcaaatat gcctattcga
     3181 aaaacgattg ttaaatcagc ttttgaagat gcgaacaact atatgaaaaa tggcgtcttg
     3241 ttacgccaag tcatcaatgt tattgatgaa gttgatttca atagccctga agatcctcat
     3301 tcgtttaatg atatttacga aaaaattctt aaagatattc aaaatgctgg gaactcagga
     3361 gaattttata cgccacgtgc agcgactgat tttattgccg aagttcttga cccaaaactt
     3421 ggagaatcaa tggcagacct tgcttgcgga acaggaggct tcttgacttc gactctgaac
     3481 cgtttaagta gtcaacgtaa aactagtgaa gataccaaaa aatataatac agctgttttt
     3541 ggtattgaaa agaaagcatt tcctcatctt ttagcagtta caaatctgtt tcttcacgaa
     3601 attgatgacc ctaaaattgt tcatggaaat actttggaga aaaatgttcg tgaatatacg
     3661 gatgatgaaa aatttgacat tattatgatg aatccacctt ttggagggtc agaattagaa
     3721 acaataaaaa ataactttcc agcagaatta cggagttctg aaacagctga tttatttatg
     3781 gctgtcatta tgtatcgttt gaaagaaaat ggtcgtgttg gagttatttt acctgatggt
     3841 tttctatttg gtgaaggtgt aaaaactcgc ttgaaacaaa aactggtaga tgagttcaac
     3901 ttgcatacga ttattaggtt gcctcatagt gtctttgcac cgtatacagg aatccatacg
     3961 aacattcttt tctttgataa aacaaagaaa acagaagaaa cttggtttta tcgtttagat
     4021 atgccagatg gttataaaaa tttctcgaaa actaagccga tgaagtcaga acacttcaat
     4081 cctgttcgtg actggtggga aaatcgtgaa gagattctgg aaggtaagtt ctacaaatct
     4141 aaatcattta cacctagtga attggctgag ttgaattata atttagacca gtgtggtttt
     4201 ccaaaagagg aagaggaaat cttaaatccc tttgagttga ttcagaatta tcaagcggaa
     4261 agagcaactt taaatcataa gattgataat gtattagctg atattttgca gttgttggag
     4321 gacaaataat gacaccagaa caacttaagg caagtattct ccaaagagcg atggaaggga
     4381 aattagtgcc gcaaaatccc aatgacgaac ctgcaagtga attattaaag agaattaaag
     4441 ctgaaaaaga aaaacttatc agtgaaggaa aaatcaaacg agataaaaag gaaactgaga
     4501 tatttcgtgg tgatgatggg aaacattatg ggaagtttgc ttatggaagt actcaagaaa
     4561 ttgatgttcc ttatgatatt cctgatactt gggagtgggt gaggttttct acattggttg
     4621 aaattgtcag aggtggctct ccacgaccaa tcaaagatta tcttacttct gaagtagatg
     4681 gaataaattg gataaaaata ggtgatactg aaaagggtga aaagtatata aataatgtta
     4741 aagaaaaaat caaaaaatca gggcttaaca aaactagatt tgtaaaaaaa ggtacatttt
     4801 tgttaactaa ttctatgagt tttggtagac cttatatttt gaatgttgat ggtgcaatac
     4861 acgatggatg gttggctatt tcgaactatg aaaactcatt aaataaagat tacctattct
     4921 atattctttc atcaaatgta gtttattctc aatttctatc tctaattagt ggagctgttg
     4981 tgaaaaactt gaatagtgat aaagttgctt ctattcttat ccctctccct ccgctagctg
     5041 aacaacaacg cataatagaa gcaatcgaat cagctttaga aaaagtagat gaatatgctg
     5101 aaagttataa tagactagaa cagctagata aaaaatttcc agataaacta aaaaaatcta
     5161 ttcttcaata tgctatgcaa ggaaaattag ttgaacaaga cccaaatgat gaatcagtcg
     5221 aagttttact tgaaaaaata cgagcagaaa aacaaaaact ctttgaagaa ggcaagatta
     5281 aaaagaaaga tttggacatt tctattgttt cccaaggaga tgata
//
LOCUS       DAVDWM010000078         5215 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVDWM010000078 DAVDWM010000000
VERSION     DAVDWM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.078"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(21..2717)
                     /gene="ppc"
                     /locus_tag="VUW49_002060"
     CDS             complement(21..2717)
                     /gene="ppc"
                     /locus_tag="VUW49_002060"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7981917.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(2737..3966)
                     /locus_tag="VUW49_002061"
     CDS             complement(2737..3966)
                     /locus_tag="VUW49_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET7981918.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(4122..4424)
                     /locus_tag="VUW49_002062"
                     /pseudo
     CDS             complement(4122..4424)
                     /locus_tag="VUW49_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(4871..5092)
                     /locus_tag="VUW49_002063"
     CDS             complement(4871..5092)
                     /locus_tag="VUW49_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7981919.1"
                     /translation="MYRRLRDLREDHDLPQKQIATILSFTNSAYAKIERGEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
BASE COUNT         1642 a         1073 c          899 g         1601 t
ORIGIN      
        1 gcattcgctc ttgaaaatta tcaacctgaa ttacgcaatc ctgtcgcaat tccgttgatg
       61 gtgatatgaa tcaatcgttc ttgatcactg gacaattctc cacgacgttg gcgtttaatc
      121 aactctaact gaatatagtt gagaatatta aagtaaggca tacggtaatc cagactagct
      181 tttagatatg gattgtcagc taagagttcg tcatgtcctt caatagccaa gataacgttc
      241 ttagtaactt gccattcatt taaaatagtc tcatagatgg ccttaacttg ctcgtcttca
      301 caaagtttag catattcaaa agcaatattc atatttgatt ttgacaaaac catatcaaca
      361 tttgaaagaa gcgattggaa gaaaggccaa ttttggtaca tatctcgtaa gatagcaata
      421 ttctctggat ttttattgat aaattccttg aagcttgaac caaccccgta ccatccaggg
      481 aacataacac gactctgtga ccatgagaat acccaaggga tggcacgcaa accaccgatt
      541 tcagtaatag tcttacgagc ggctggacga gaaccaatat taaaacttga aatagccttg
      601 attggacttg actcgaagaa ataatcatag aaatgctcat taccaaagac caaatcacgg
      661 tagatatcgt aactacggtc cactacttga tccataatgg cttcataacg atttggggta
      721 ttggtatcgc tcttcttctg agtaatcata cggttaatag ctgccgatac tagcatttca
      781 aggttatagt aagcggcgtc tttgttaccg tatttattcc caattacttc accctgctcc
      841 gtcaagcgga tacgatcctt gatagacttg agcggttgag atgtaatggc ttcataggtt
      901 ggcccaccac cacgaccgac agtaccacca cgaccatgga agaaggtaac cttaacgcca
      961 aattcatctc caatagcagt caattgctgt tgagccttgt agagggtcca acatgatgac
     1021 aagtaaccgc catctttatt actgtcagag tagccaagca tgatttcttg gtagttattt
     1081 cgtgagtcaa tccatttttt ggcaaggcta agagaaagat attttctcat tgtttcctct
     1141 gaatgatcca agtcttcaat tgtttcaaaa aggggaacaa tctgaacacg cgccctttcc
     1201 gtatccacca gtcctacttc ttttaacaga atagctaatt ctagcatatc agaaaggctg
     1261 gttgcatgtg aaatgatggt ctgacggatg acatcatctc ccaacttatc tttcaaaaca
     1321 cgagccgtct taaaaatagc taattctttt gctaataatt ctgatttttc tgcgtgagtc
     1381 gcagaaagaa ttcggggatc ttcttctaat tctttcaaga gaaggtcaca cttttcttct
     1441 tcgctcaact cgctataacg agaatgaatt cctgctgatt tcaagagttc tgccacacag
     1501 gcttcataga cgctagagtc ttgtcgcata tcaattgacg ctaagtaaaa accaaatatc
     1561 tctactgcct gcaataattc cacaaaatca cctgaaatca aggactcacc cttattttct
     1621 agtagagaat ctcgaatggc aatcaaatcc ttgtagaaat cattggcggt ttcataacga
     1681 gtcccaactt cttcatcctc aatcagatag gttttagttg cctgaatttt tgattgaata
     1741 tcaaacaagg cacgacggta aagctctttt tcgcggtaaa tcgagttatc cttggattga
     1801 cgagccattt ctctgacttg cttgctgacg ttgacaatgc tagttgaaag agaaaattca
     1861 cgataaagtt ggtaaatctt tttatcatag tagttcatga tgacttcaca ctgagtgagt
     1921 gcagactgct tcaaggtctt tgctgtaaca aatggatttc catcacggtc accacctatc
     1981 cacataccca tggtgattgg tttagcctgt tttaaattca gaccatgcgc ttgcgctaag
     2041 cgcttatact ccgtcgtcaa atgaggtaca gctttcaaaa aggagctgtt ataatattcc
     2101 atagcattcg tgatttcgtt agtcactttt aattttttct cacgaatcat gtctgtctgc
     2161 atgataattt cgatgtaacg acgcaaatca ttgtaccatt tatctttatt aatcaacccc
     2221 aacttaacat cacggtattt acgcaaaaga ctatgaatat gatttgttaa atccaacata
     2281 cttttgcgtt gcacttgtgt tggatgggct gtcaaaacag ggacaacatt caagtgttct
     2341 aggatctcaa cggcattttc cttttctgct accaatttaa tcgttgtaga taatttacct
     2401 aaatagtcct gatcaatatt attttgatga ttgatttcat aagctaaatc cacatcctct
     2461 gaaatattaa tcaaaagagg caagatagag aaatagcgtg aaatatagac catttcatca
     2521 tttgataagc tagtcactag acggtttaga ccttgataat cttcctgcgt tgataattct
     2581 ttcaactgta ttattttttc aaaggtctct ggggcaagca tatttttagt aatatcttcc
     2641 agtaattctg ttagaatcaa gacttcttct tgcacaacac ttttattact ataattttct
     2701 aatttttgaa gagacataaa ctatcctttc tttatcctac ttcaacagaa ggttcattgg
     2761 ttgattttcc aattctcggt acaatttagc gcgtttttca ctggcatcaa tatttaagac
     2821 aaaggctact gccactgata agactagaag actatttcca ccctgggata agaaggggaa
     2881 ggttactcct gtagatggaa tcaagcccga aatccctccg atattgacaa atacctgaac
     2941 caacatcatc cctccgacac cgagtgcaac catggcattg aaaggattct ccgctcggat
     3001 accgaccaag ataatccgca aaatcatgaa aaataagaga gctaaaataa gactggcacc
     3061 aacaaagcca aattcttcaa tcacgataga aaagacaaag tctgtatgag cttctggcaa
     3121 ataacctcgt ttttcaatcg agtttccaag acctagacca aaccaaccgc cattgaccat
     3181 ggcaaaataa gaattagcta actggtgacc tgcatcagca cgatcggcaa aaggattaaa
     3241 aaaggcacta aagcgcttgg ctacatagcc aaatactgga atttttgaaa aggtctcaac
     3301 accgattagg ctgatagtgg tcaagacaaa gacagaagcg gcagatacga gcgccagaat
     3361 ggttgaaaac cagcgataag cgattccact aactgtatac ataatcaagg aaaccaagac
     3421 taaaatagtc gcatttccta aatcagggaa aattcccaaa cttccaatca gaactaggag
     3481 aacgaatcgc caatcattaa aagcacgggg aagccattga ttttgagtca aaacttgaaa
     3541 atcataagta gctatttctt cttgctgttt ggagaatcgg tgagctaaat accaaataat
     3601 aatgattttt aagtactcag ctggctgaat agttattcct gcaaccgaaa tccaaccgta
     3661 tgccccgttt acggaaatac caataaaacg agccaagaac aataaaagca tttctattaa
     3721 tataactaaa atgattagtc gctcatttct caaaaaatct agtctcaatt tataaattaa
     3781 ggcaatcagt atcaaactaa caatccaaaa gattccttgg tttcgaacca actgcaaggc
     3841 actcttgcct tcttcaatta aaatagcact ggtggtcgaa tagaccacaa tcaagcccaa
     3901 aatagataaa agcaagtagg gaatcaagat ggaataattt aataagtgcc tcttactaat
     3961 cttcatagca tcaccactct actaggatac agacaaatct gttccaaatt ccgttttatt
     4021 ttctagtttt gattgctatt ataccatttt ttcagaaaga aaaaaactct taccttacaa
     4081 tccttcgaat tttactgttt ttacagaatt aaggcagaat ttgaaatcgt aaaaccattt
     4141 tgaataagag ttgcttcaac ccattgactc cgacggatta agttgctttc gtgaatgcca
     4201 aaatcagccg caatttgttc ataagtgctc tattctcgca tgtattgaat agttaccata
     4261 aggagatcgt ctaggcttaa tttgcttttt cgtccacctt ttgcgcgttt acgttgataa
     4321 gctgttttta acacagctaa catctcttca aaagtcgtgc gctgaacacc tacaaggatc
     4381 ttgaaacggc tatcgctcaa ttgtttactt gcttcatcat tcatagtttt attgtattat
     4441 atttttgttt cgcagaaagt ctattattcc tttcgttcca aggtttttac gcttttagca
     4501 acaagcttac acactccacg tgatgcgtct gtagaactca aaaggttcgg ggaacctttt
     4561 gaggattaat tgcgtttatc taacaaactc acacattcaa cgtggcatga ctgatgaagt
     4621 catcataaga atttcacttg ctgctctttt acgttggcag gctgaacggt cagaagtatc
     4681 attgtatatc atttgtatca tggcatataa agtatcgctc tattttattg atggttttaa
     4741 aatcgctctt atcatggcga gccattcaaa tttgctccac aaatgaggaa agtaccattt
     4801 taggctattc agttgtcaaa gggcaaataa aaaatctcac tcattttcaa actctatgaa
     4861 attgaggaga ttattttcta aagcgaattt tatcaggaaa atcagttaat cccaataaat
     4921 agtctgtact gacgtcatag aaatctgaga gtttaaccaa tacatcagcc gtcaacgcat
     4981 gctcaccccg ttcaattttg gcataagctg aatttgtaaa cgaaagtatt gtagctattt
     5041 gcttttgggg cagatcatga tcctccctca aatctctcaa acgtctgtac atctagcttt
     5101 ctcctaagaa aaagtataga ccatatattg taactattta ggatattgtc caaatcggac
     5161 aacaggaatt ttaccatcct ttatatagac ttttattttt atgtatcaag ttttt
//
LOCUS       DAVDWM010000079         5149 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVDWM010000079 DAVDWM010000000
VERSION     DAVDWM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.079"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(44..190)
                     /locus_tag="VUW49_002064"
     CDS             complement(44..190)
                     /locus_tag="VUW49_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981920.1"
                     /translation="MKTKEQTRKLAVGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            348..566
                     /locus_tag="VUW49_002065"
     CDS             348..566
                     /locus_tag="VUW49_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET7981921.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            1611..2603
                     /locus_tag="VUW49_002066"
     CDS             1611..2603
                     /locus_tag="VUW49_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7981922.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2605..3423
                     /locus_tag="VUW49_002067"
     CDS             2605..3423
                     /locus_tag="VUW49_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET7981923.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3425..4210
                     /locus_tag="VUW49_002068"
     CDS             3425..4210
                     /locus_tag="VUW49_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7981924.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4308..4526
                     /locus_tag="VUW49_002069"
     CDS             4308..4526
                     /locus_tag="VUW49_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET7981925.1"
                     /translation="MKDLPEWFGIPESTQTYIEGTTTLQVWTAYQESDLTGFVSLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4511..4708
                     /locus_tag="VUW49_002070"
     CDS             4511..4708
                     /locus_tag="VUW49_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7981926.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1471 a          870 c         1066 g         1742 t
ORIGIN      
        1 tcgaaatcta gtggaatgaa tctggaatag tccatcgagc tttctaatac tctttgaaaa
       61 tctcttcaaa ccacgtcaac gtcgccttgc cgtgcgtatg gttactgact tcgtcagttc
      121 tatccacaac ctcaaaacag tgttttgagc aacctacggc tagtttccta gtttgctctt
      181 tggttttcat tgagtataac acattgttag aagttggttt aaattcccta atcaatttgt
      241 tcacatttac cttcgatata ttatatccca tagttaaggt tggtcatgca gatgattata
      301 gtcatggagc cgtaaaactt agtgtttctt tagttgacaa agatgccatg aaaaaaatat
      361 ctgtaactgt aataggatat tttgaaataa atatagatga aaatatcacc gatattctat
      421 acgtaaatgg tactgctatt ctttatcatt atttacgttc tattgtttca atagtttcgg
      481 caattgatag cagtgaagca atgttgctac ctaccattaa tgttttagag ttactagata
      541 aatctcaacc ttttgaagaa gaataattta ttagctcact aaattgaggg taaggaaaag
      601 taaaatcagt aagaaaaatg tcttgcatta tacagcaacc ttttgggaat gagtagatgg
      661 attgaataaa attttattaa gagtggatga tttatctgta gattattatt ggacagttag
      721 tcttgaagta gtctaagaat taggttataa tcagtagaat ccttgctaat aatgaggagg
      781 ttagtttatg tatagtagac tgaatctaaa atagtacgaa acaattgcta aaacatttat
      841 agaaattaat tttactttcc caatcgattt gttctcatct tatttcaatc cgctatatat
      901 tatggtatcg aatcttcatc agaatgataa aattaatcaa ttgatatctg attacaaaca
      961 gaatatgaaa gctttttata tcactattga aaaatttata cgagatgatg aaagccttaa
     1021 gtgttatttt ataaaggtta tttcaagtcg ttccaaggta acaagtctag atcagattga
     1081 agctgataaa acgatacaaa gaaaatattc aagtgagcta aaaaaattta ttggatttta
     1141 taatgagatt atttgtgagg aaaatagttt cctacatgta cgaaagaggt ggtcgagttg
     1201 gtttaggtag tcgatgcgtg agttgataat tctcagggta tggacttctt tttcatgaat
     1261 gaggtaaaag agcaggtatt gtttagagac aatcattctg agcatatttt ctggatagag
     1321 ggagtatccg attttatgat caaagttaat accgccctct ggtgagaaga tgagtaggtt
     1381 ggtaatttaa actattaaac agaatttttg attaaaagta ttattttatg agagaaatcc
     1441 taatttcaca atccataggc aaactcttgc atttcgtttt ttattggact ataataggtt
     1501 ggtataaagc cttttgtagt aataaaatgt agaaggtgta gaaagcaagg atttagaata
     1561 tttgtagtta aaaacacaat gttgctattc cttacgatag ggagatagat atggcaatga
     1621 tagaagtgga acatcttcag aaaaattttg tgaagactgt taaggaaccg ggcttgaagg
     1681 gggctttgcg ctcctttatt catcctgaaa agcagacctt tgaagcggtc aaggatttga
     1741 cctttgaggt tccaaaaggg cagattttag gatttatcgg ggcaaatggt gctgggaagt
     1801 cgacaaccat taaaatgctg acaggaattt tgaaaccaac atctggtttt tgtcggatta
     1861 acggcaagat tccccaggac aatcggcaag attatgtcaa agatattggc gtagtctttg
     1921 gacaacgcac ccagctatgg tgggatttgg ctctgcaaga gacctacact gtcttaaaag
     1981 agatttatga tgtgccagac tcgctctttc ataagcgtat ggactttttg aatgaagtct
     2041 tggatttgaa ggactttatc aaggatcccg tgcggactct ttcactggga caacggatgc
     2101 gggcggatat tgcggcctcc ttgctccaca atcccaaggt tcttttttta gatgagccga
     2161 ccattggttt ggacgtttcg gttaaggata atattcgtcg ggcaattact cagatcaatc
     2221 aagaggaaga aactaccatt cttttgacca ctcacgattt gagtgatatt gagcaacttt
     2281 gtgatcggat tttcatgatt gacaaggggc aagagatttt tgatggaacg gtgagccaac
     2341 tcaaggagac ctttggtaag atgaagactc tctcttttga actgctacca ggtcaaagtc
     2401 atctcgtctc tcactatgaa ggtctgtctg atatgaccat tgatagacaa ggaaacagcc
     2461 tcaacattga atttgatagt tctcgctacc agtcagctga cattatcaag caaaccctgt
     2521 ctgattttga aatccgcgat ttgaagatgg tggatacgga tattgaggat attatccgtc
     2581 gcttctaccg aaaggagctc taggatgatc aaattgtgga gacgttataa accctttatc
     2641 aatgcagggg ttcaggagtt gattacttac cgagtcaact ttattctcta tcggattggc
     2701 gatgtcatgg gggcttttgt ggccttttat ctctggaagg ctgtctttga ttcttcgcaa
     2761 gagtctttga ttcagggctt cagtatggcg gatatcaccc tctacatcat catgagtttt
     2821 gtgaccaatc ttctgactag atccgattcg tcctttatga ttggggagga ggtcaaggat
     2881 ggctccatta tcatgcgttt gttgcgacca gtgcattttg cggcctccta tcttttcacc
     2941 gagcttggtt ccaagtggtt gatttttatc agcgttggcc ttccattttt aagtgtcatt
     3001 gtcttgatga aaatcatatc gggtcaaggt attgtagagg tgctaggatt aactgtcctt
     3061 tatcttttta gcttaacgct cgcctatctg attaactttt tctttaatat ttgctttgga
     3121 ttttcagcct ttgtgtttaa aaatctttgg ggttccaacc tacttaagac ttccatagtg
     3181 gcttttatgt cggggagttt gattcccttg gcattctttc caaaggttgt ttcagatatt
     3241 ctttcctttt tgcctttttc atccttgatt tatactccag ttatgatcat tgttggaaaa
     3301 tacgatgcca gtcagattct tcaggcactc cttttgcagt tcttctggct cttagtgatg
     3361 gtgggattgt ctcagttgat ttggaaacgg gtccagtcct ttatcaccat tcaaggaggt
     3421 tagtatgaaa aaatatcaac gaatgcatct gatttttatc agacaataca tcaaacaaat
     3481 catggaatat aaggtggatt ttgtggttgg tgtcttggga gtctttctgg ctcaaggctt
     3541 gaatctcttg tttctcaatg tcatctttca acatattcca ttcctagaag gttggacctt
     3601 tcaagagata gctttcattt atggattttc cttgattccc aagggaatgg accatctctt
     3661 ttttgacaat ctctgggcac tagggcaacg cctagtccga aaaggagagt ttgacaagta
     3721 tctgactcgt cccatcaatc ctctctttca catcctagtt gaaacctttc agattgatgc
     3781 cttgggtgaa ctcttagtcg gtggtatttt attgggaaca acagtgacca gcattgtttg
     3841 gactcttcca aaatttctgc ttttcctagt ttgtattcct tttgcgacct tgatttatac
     3901 ttctcttaaa atcgcaacag ccagtatcgc cttttggact aagcagtcag gcgccatgat
     3961 ttacatcttc tatatgttca atgactttgc taagtatccg atttctattt acaattctct
     4021 tcttcgttgg ttgattagct ttatcgtgcc tttcgccttt acagcctact atccagctag
     4081 ctatttctta caggaaaagg atgtgttctt taacgtagga ggtttgatgt tgatttctct
     4141 ggttttcttt gttatttccc ttaaactttg ggataagggc ttagattcct acgaaagtgc
     4201 gggttcgtaa aagctaaagt aagactaaaa tcaagaaagg aacttatgat gtttgtaatt
     4261 gaagaagtca aggatgaaaa tcaaaaaagg cagttgtcgc tgagattttg aaggatttgc
     4321 cagaatggtt tggaatccca gaaagcacac aaacctatat agaaggaacc acgacactgc
     4381 aagtttggac cgcctatcag gagagtgatt tgactggatt tgtaagctta tcctattcga
     4441 gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag gtagaaaaat
     4501 tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg gttatctgca
     4561 ggtcaaaaca gtggcagaag cttctaataa agattatgat cgaacaaatg acttttatcg
     4621 aggtcttggc tttaaaaagt tagagatttt tcctcaacta tggaatccgc aaaatccttg
     4681 tcagattttg attaaaaagc ttgaataata ttacttgaca tctatttttt attctcagag
     4741 tgctatactg taagtgtaat cgccgattta gctcagttgg tagagcaagg cactcgtaaa
     4801 gcctaggtta taggtagata aacgactgag gagttgaaaa aatagatagg tagaagataa
     4861 ccgttaagcc ttattcttag cggttattta tattgtttaa tagcgctgat atttcatcaa
     4921 ttatgcctgt tttcgtgttt ccggtagttg ttcaagttta ttgctactat ttttgatggt
     4981 atgaatgtgc ttataatgta tcccggttaa cgaaagtttt ggacttatac tcttagaaaa
     5041 tctcttcaaa ccacgtcaac gccgccttgc cgtgcgtatg gttactgact tcgtcagttc
     5101 tatccacaac ctcaaaacag tgttttgagc tgacttcgtc agttctatc
//
LOCUS       DAVDWM010000080         5078 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVDWM010000080 DAVDWM010000000
VERSION     DAVDWM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.080"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            103..615
                     /locus_tag="VUW49_002071"
     CDS             103..615
                     /locus_tag="VUW49_002071"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET7981927.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            797..1741
                     /locus_tag="VUW49_002072"
     CDS             797..1741
                     /locus_tag="VUW49_002072"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET7981928.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            1750..2427
                     /locus_tag="VUW49_002073"
     CDS             1750..2427
                     /locus_tag="VUW49_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET7981929.1"
                     /translation="MTYLDAFKSGNLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTSQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            2525..3283
                     /locus_tag="VUW49_002074"
     CDS             2525..3283
                     /locus_tag="VUW49_002074"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7981930.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            3321..4825
                     /gene="lytC"
                     /locus_tag="VUW49_002075"
                     /pseudo
     CDS             3321..4825
                     /gene="lytC"
                     /locus_tag="VUW49_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
BASE COUNT         1572 a          857 c         1174 g         1475 t
ORIGIN      
        1 atcgtcaaaa cgttagataa ttattgaagt taacttttcc ttttttcttt cttaaaaaat
       61 atgctataat agagagtaaa aaactttgaa agaaagaaaa agatgaattt aaaagattac
      121 attgcaacaa ttgaaaatta tccaaaggaa ggcattacct tccgtgatat tagtcctttg
      181 atggctgatg gaaatgctta tagctacgct gttcgtgaaa tcgttcagta tgctactgac
      241 aagaaagtcg acatgatcgt gggacctgaa gctcgtggat ttatcgtggg ttgtccagtt
      301 gcctttgagt tgggaattgg ttttgcgcct gttcgtaagc caggtaaatt gccacgcgaa
      361 gttatttctg ctgactatga aaaagagtac ggtgtcgata ccttgactat gcacgcggat
      421 gccattaagc caggtcaacg tgttcttatt gtagatgacc ttttggcgac aggtggaact
      481 gttaaggcaa ctatcgagat gattgaaaaa cttggtggtg ttatggcagg ttgtgccttc
      541 cttgttgaat tggatgaatt gaacggccgt gaaaaaattg gtgactacga ctacaaagtt
      601 cttatgcatt attaatgaaa acagtcccta gggctgtttt ctctacacta ggatataaaa
      661 atagactata actagttaga gaaaaactat aattgaaaac tatatcttct tacagtataa
      721 taaaaggact aagtgtttga gatttgtctt caaacatatg caattattcc tgaaagagta
      781 cagttaggag agggttatgc cgattcgaat tgataaaaaa ttgccagctg ttgagatttt
      841 acggacagag aatatctttg tcatggatga tcaacgtgct gcccaccaag atatccgtcc
      901 tttgaagatt ttaattttaa atctcatgcc acagaaaatg gtcacagaga cccagttgtt
      961 gcgccacttg gctaatacac ccctacaact ggatattgat tttctctata tggagagcca
     1021 ccgttctaaa acaactcgtt cagagcacat ggagaccttc tataaaactt ttcctgaagt
     1081 caaggatgag tattttgatg ggatgatcat cacgggtgct ccagttgagc atttaccatt
     1141 tgaggaagtg gactattggg aggaatttag acagatgctt gagtggtcta agactcatgt
     1201 ctattcgacc cttcatatct gttggggggc tcaggctggg ctttatctgc gctatggtgt
     1261 agaaaaatac cagatggaca gtaagctatc aggtatttat cctcaggaca ccctaaaaga
     1321 gggtcacctt ctatttagag gctttgatga tagctatgta tcccctcatt cacggcacac
     1381 ggagatttct aaggaagagg tcttaaacaa gaccaatctc gagattttat cagaaggacc
     1441 tcaggttggg gtttctattt tggccagtcg tgatttacga gaaatttata gttttggtca
     1501 tttggagtat gaccgtgata ccttggcaaa agagtatttt cgagatcgtg atgcaggttt
     1561 cgacccacat attccagaaa attactttaa agatgatgat gttaatcagg taccttgtct
     1621 ttgttggtct tcatctgcag ccctcttttt cagtaattgg gtaaaccatg cggtctatca
     1681 ggagacgcct tttgactgga gaaagataga agatgatgca tctgcatatg ggtatttata
     1741 agaggaatta tgacatattt agacgctttt aaatcgggga acttggtttt accgagtgcc
     1801 ctgctcttgc attttaagga actctttcct tctagcgacg attttctggt ttggcaattt
     1861 ttctatttgc aaaatacgac aggcttagaa gaaatgtcgc caagccagat tgctgaaagg
     1921 attggcaagg aaatttcgga tgtcaaccag tccatttcta atctgacgga aaggggactg
     1981 ctccagtatc gtactatcga attaaatggc gaaattgaat tgctctttga tgctagtttg
     2041 gccttggaac gtttggatga cctgtttgga gcagttcatt caagttcaga ccagctgaca
     2101 tctcaaaacc agctcaagga tttggtggaa accttccagc aggagttggg acgattgttg
     2161 acgccttttg agattgagga tttgaccaag acactaaagg aagatggaac cagtgctgac
     2221 ttgattaagg aggctcttcg tgaagctgtt ttgaatggaa aaccaaactg gaagtacatt
     2281 caggcgattt tgagaaactg gcgccatgaa ggaatcaaga gtgtggctca aattgaggcc
     2341 aagagagcag aaagagaagc aagcaatcct cagttgacac aggtatctgc agatttcaga
     2401 aatgccatgg atctctggaa ggattaatcc atgcaagcag gcttgaaatc cgagtaagat
     2461 ttgcaagctg tgtataattg tgatagaata aatagaaaat aaattgaaaa aagaggtatg
     2521 taaaatgtca cgtaaaccat ttatcgctgg taactggaaa atgaacaaaa atccagaaga
     2581 agctaaagca ttcgttgaag cagttgcatc aaaacttcct tcatcagatc ttgttgaagc
     2641 aggtatcgct gctccagctc ttgatttgac aactgttctt gctgttgcaa aaggctcaaa
     2701 ccttaaagtt gctgctcaaa actgctactt tgaaaatgca ggtgctttca ctggtgaaac
     2761 tagcccacaa gttttgaaag aaatcggtac tgattacgtt gttatcggtc actcagaacg
     2821 ccgtgactac ttccatgaaa ctgacgaaga tatcaacaaa aaagcaaaag caatctttgc
     2881 gaacggtatg cttccaatca tctgttgtgg tgaatcactt gaaacttacg aagctggtaa
     2941 agctgctgaa ttcgtaggtg ctcaagtatc tgctgcattg gctggattga ctgctgaaca
     3001 agttgctgcc tcagttatcg cttatgagcc aatctgggct atcggtactg gtaaatcagc
     3061 ttcacaagac gatgcacaaa aaatgtgtaa agttgttcgt gacgttgtag ctgctgactt
     3121 tggtcaagaa gtcgcagaca aagttcgtgt tcaatacggt ggttctgtta aacctgaaaa
     3181 tgttgcttca tacatggctt gcccagacgt tgacggtgcc cttgtaggtg gtgcgtcact
     3241 tgaagctgaa agcttcttgg ctttgcttga ctttgtaaaa taatcagtaa gtagcaaaag
     3301 ctaggtggaa cagcattcag atgtctgtta cattttttat aggagagaaa gattgaaaac
     3361 aaaaattgga ttagcaagta tctgtttact aggcttggca actagtcatg tcgctgcaaa
     3421 tgaaactgaa gtagcaaaaa cttcgcagga tacaacgaca gcttcaagta gttcagagca
     3481 aaatcagtct tctaataaaa cgcaaacgag cgcagaagta cagactaatg ctgctgccca
     3541 ctgggatggg gattattatg taaaggatga tggttctaaa gctcaaagtg aatggatttt
     3601 tgacaactac tataaggcct ggttttatat taattcagat ggtcgttact cgcagaatga
     3661 atggcatgga aattactacc tgaaatcagg tggatatatg gcccaaaacg agtggatcta
     3721 tgacagtaat tacaagagtt ggttttatct caagtcagat ggggcttatg ctcatcaaga
     3781 atggcaattg attggaaata agtggtacta cttcaagaag tggggttaca tggctaaaag
     3841 ccaatggcaa ggaagttatt tcttgaatgg tcaaggagct atgatgcaaa atgaatggct
     3901 ctatgatcca gcctattctg cttattttta tctaaaatcc gatggaactt atgctaacca
     3961 agagtggcaa aaagtgggcg gcaaatggta ctatttcaag aagtggggct atatggctcg
     4021 gaatgagtgg caaggcaact actatttgac tggaagtggt gctatggcga ctgacgaagt
     4081 gattatggat ggtgctcgct atatctttgc ggcctctggt gagctcaaag aaaagaaaga
     4141 tttgaatgtc ggctgggttc acagagatgg taaacgctat ttctttaata atagagaaga
     4201 acaagtggga accgaacatg ctaagaaaat cattgatatt agtgagcaca atggtcgtat
     4261 caatgattgg aaaaaggtta ttgatgagaa cgaggtggat ggtgtcattg ttcgtctagg
     4321 ttatagcggt aaagaagaca aggaattggc gcataacatt aaggagttaa accgtctggg
     4381 aattccttat ggtgtctatc tctataccta tgctgaaaat gagaccgatg ctgagaatga
     4441 cgctaaacag accattgaac ttataaagaa atacaatatg aacctgtctt accctatcta
     4501 ttatgatgtt gagaactggg aatatgtaaa taagagcaag agagctccaa gtgatacaga
     4561 tacttgggtt aaaatcatca acaagtacat ggacacgatg aagcaggcgg gttatcaaaa
     4621 tgtgtatgtc tatagctatc gtagtttatt acagacgcgt ttaaaacacc cagatatttt
     4681 aaaacatgta aactgggtag cggcctatac gaatgcttta gaatgggaaa acccttatta
     4741 ttcaggagaa aaaggttggc aatatacctc ttctgaatac atgaaaggaa tccaagggcg
     4801 cgtagatgtc agcgtttggt attaagcaat gatttgaaag agggatgtga tagtagcacc
     4861 ctctttttct ttgttttatg atagttcatc ctcgagtaaa ttcaagatct tgctcggaaa
     4921 tgaagcttat atagtagatt gaatatagac aaataccttg tgattggtaa aacattttag
     4981 aaattcatgg ctctttgtca actgtagtgg gttgaaaaaa agctaagctc gagaaaggac
     5041 aaattttgtc ctttcttttt tgatattcag agcgataa
//
LOCUS       DAVDWM010000081         3497 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVDWM010000081 DAVDWM010000000
VERSION     DAVDWM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.081"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            232..393
                     /locus_tag="VUW49_002076"
     CDS             232..393
                     /locus_tag="VUW49_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981931.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            485..2164
                     /locus_tag="VUW49_002077"
     CDS             485..2164
                     /locus_tag="VUW49_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7981932.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLHIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENISIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            2432..2998
                     /locus_tag="VUW49_002078"
     CDS             2432..2998
                     /locus_tag="VUW49_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981933.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3320..>3497
                     /locus_tag="VUW49_002079"
     CDS             3320..>3497
                     /locus_tag="VUW49_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET7981934.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIY"
BASE COUNT         1322 a          441 c          747 g          987 t
ORIGIN      
        1 tctgctaagc aaaaagaaag tgagcgataa gtaagagttt gaatataggg agggataccc
       61 tatccgctat gaagaaaatg aggataatga tacttctaag gttgaagccg gctcatccgg
      121 aatagaggcg gaggtgagat tcctatgttg ctaatccaaa gcacttgtca atgtcaaaaa
      181 acttaattaa acaaataatt attagatata aaactttaga ggaggtatat attggacaat
      241 gactggaatg gattggctga tttaatagca aatctgattg caaaatatgc tggtgcttta
      301 gatttagata atctacccga tccaacgcca gcaaaaaatc aagagatgaa aaatagtttt
      361 gacatggcaa aaacacaaat tgagacggac taataaaagt gttataatat acttgaaatg
      421 atagtccaaa cttaaattaa aaaaattata tatgatatag aaaaattata ttgagattaa
      481 tactatgtca aaagaaaaaa taaaagtata cctctataca cgagtatcta cgtcaataca
      541 gatagagggg tattcgttag aggcacaaaa atcacgaatg aaagcttttg ctatttacaa
      601 cgattatgaa attgttggag agtatgaaga tgccgggaag tctggcaagt ctattgaagg
      661 tagaatacag ttcaatcgaa tgatggaaga tataaaatct ggaaaagatg gagtatcttt
      721 tgttcttgtg tttaagttat caagatttgc aagaaatgcc gctgatgttc tttctacact
      781 tcatataatg caggattatg gagttaatct tatctgcgtt gaagatggca ttgattcatc
      841 taaagatgct gggaaactaa tgatttctgt tttatcagct gtggctgaaa tagaaagaga
      901 aaacatcagt attcaaacaa tggaaggtcg cattcaaaaa gcaagggaag gaaaatggaa
      961 tggtggcttt gctccgtatg gatataaact tgaagacggc aagctgttta taaatgagga
     1021 agaggcagtt gcaataagaa cgattttcga ccagtatgta aatactacga taggagccaa
     1081 tgggatatct aaatacttag agaatcatgg aattagaaaa atcccaagac agaatggtaa
     1141 gaatcctttg tttgatgcag gtcttataag aaagatatta aagaatcctg tatataatgg
     1201 gaaaatagca tttggaagaa gaactttaga aaaagttcat ggtacaagaa atgaatataa
     1261 gcaggttgaa caagatgaat atctaatatc tgaagggata catgaagcta tagtttccga
     1321 tgaagtttgg caagctgctc aggttaagct aaaatctcaa gcaaagaaat atgagcatgt
     1381 gaataaagga aaagatacac gcacacactt gctttctgga attgtaaaat gcccgatatg
     1441 tggagtggga atgtttggga acaagtgtat caagaaaaag aaagatggca caaagtataa
     1501 agatttttat tactatggtt gtaaccatag gcagatgata agaggtcata agtgtacatt
     1561 cagtaagcaa attagagaag aattgttaga tgatgctgtt gcagaggtga ttgtcaagat
     1621 agtaagtaat ccgaaatttg cttctatgat gcaagaaaaa atcaacatga aggtggatac
     1681 ctctgaaata gaaaaagaaa tagataatta ccaaaaggaa ttgagaaaga gtcattctac
     1741 aaagtttaag ctaattgagg aaatagataa tttagatgtt gaagataagc attataagag
     1801 aaggaaacag gatttagatg atagactata tcgtatgtat gacaaaatag atgaattaga
     1861 atcatcacta attgatgcga aagcaaagaa acagactatt gaagctgaaa agcttacagg
     1921 agataacata tataaggttc tgatctattt tgataagctc tataaagtca tgaatgatgt
     1981 agagcgtagg cagttaattt cagctttgat ttctgaaatt caagtttatg aagaaaaaca
     2041 atctaacgga caatggctaa aatccattac ttttaagcta ccaattatag aagaaaatct
     2101 caacatagat ttggacaatg atgaacaagt tgaatgtgta agtttattag agaaacgtag
     2161 ttaatcctca aaaggttccc caaacttttt gagtcccact gacgcatcac gctgaggcgg
     2221 tatcactgct tgtacgagtt gaagcatcag cgaagtaaaa gtagatgttc agccaatgcg
     2281 acgaggtgag accgaaggtc gataaggtgt gttctgggta taggactcgt aaagtaagta
     2341 ggaaatatga tgagatgata cagagagcct gaattggtca ggtgacgtgc ttatttacat
     2401 tagcctgata atgaaaatgg agagattatc gatggttata ttggaattgt accaaaatga
     2461 ttattctaaa gatttagtag catttgattc catagaagat gggaaagcct ttgtagctca
     2521 aatcccggga tacactctgg aaacagaaga tggttttgaa gtggagtatt ttaatcctaa
     2581 aaacataccg gattatatgg aaattatttt taacggaaat attgttcctt tatctaaatt
     2641 tatgttcgat cctggggaaa atgtaaacat tatttggaaa gagatttcaa atttatccct
     2701 caaaaatgat agagtgatcg aaggatattc aaaaattgat gcatatgtcg ttaacaacca
     2761 tgaagttaaa gcctatgtcg aaacaagaga aacgaactat cgtaaagcaa aagacttctt
     2821 agaaagccgc ggatatgaaa tcgacagaag ctttttcgga agtgaagacg gtgaagcgat
     2881 tctttacaga aaaaaaggga tcgaagtttg gcatttcttg tgtcacttag acccaatgtt
     2941 tgtagagatt gaagatgtag aaggatatgt taaagaagaa gttggagaga ttcaatagtc
     3001 aaccaaagtt ccttgtgagt atataccttt gctagtaaaa gagtcaaaac gttataacga
     3061 aggggggtaa gaaaaccctt gagtgtaagt ctttttcttg tttagaggga aagtattaga
     3121 gtagccgctc ataatttata ctagatttga taactgattt gagacagatt ttatttctat
     3181 aaatattgtg aaaatttttt aataccaaag atttaaagtt actgtgtaaa aaatcctctc
     3241 cacttttaaa ctaatgtatt ataaaagcca gttgacacaa gtgttacacc tgtgatagaa
     3301 ttaacttgag gtgatgaaaa tggcaaccga taaaaataga ataatgatta gcttggatga
     3361 taaaaatcta gagaaacttg aaaatttagt agaagatgct agagatagaa ggggaatgcg
     3421 actaacaaag tcccaggtta ttgaattact cctaaatacc gttgattatt tcgatgatat
     3481 tatgggggct atttact
//
LOCUS       DAVDWM010000082         3228 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVDWM010000082 DAVDWM010000000
VERSION     DAVDWM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.082"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            291..1388
                     /gene="glf"
                     /locus_tag="VUW49_002080"
     CDS             291..1388
                     /gene="glf"
                     /locus_tag="VUW49_002080"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014634450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HET7981935.1"
                     /translation="MYDYLIVGAGLSGAIFAYEATKRGKKVKVIDKRNHIGGNIYCEN
                     IEGVNVHKYGAHIFHTSNKKVWDYVNQFAEFNNYINSPVANYKGSLYNLPFNMNTFYA
                     MWGTKTPQEVKNKIAEQTAHMKDVEPKNLEEQAIKLIGPDIYEKLIKGYTEKQWGRSA
                     TDLPPFIIKRLPVRLTFDNNYFNDRYQGIPIGGYNVIIENMLKDVEVELGVDFFANRQ
                     ELEASAEKVVFTGMIDQYFDYKHGELEYRSLRFEHEVLDKENYQGNAVVNYTEREIPY
                     TRIIEHKHFEYGTQDKTVITREYPADWKRGDEPYYPINDERNNAMFAKYQEEAVQNDK
                     VIFCGRLADYKYYDMHVVIERALEVVRNEFS"
     gene            1706..2386
                     /locus_tag="VUW49_002081"
     CDS             1706..2386
                     /locus_tag="VUW49_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009731852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HET7981936.1"
                     /translation="MNNRIVDISLFDLLNVLWLRKWIIVFVSFVFGCISFLYYQFFVP
                     KTYTSDTRIYVVSKGDNKNLSNADLQAGSALTKDYKEIILSDEVLEETISDLKLESTL
                     ESLASKIKISIPAETRIISISVTNTNSDEAARIANGIRKVAALKIKEVTQVTDVTTLQ
                     TARPPQTPSGPHVRKSTTAGLVLGAFLTVFLVVAKEILDDRIKRFEELEKLGIPILGS
                     IPLSKNIK"
     gene            2393..3082
                     /locus_tag="VUW49_002082"
     CDS             2393..3082
                     /locus_tag="VUW49_002082"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET7981937.1"
                     /translation="MGKLELTRKYYQLYNETKEYFNALQTNIQLSGDGLKVISVTSTV
                     KNEGKSTISVGLSMSLALSGYKVLLLDVDLRNSVMSGMFHSRERITGLTEYLSGKAGM
                     ADSMQETTLDNLYILQAGVVSPNPLSLLRSVKFETLIDSMKKYFDYIIVDTPPIGQVI
                     DAAIIAQQCDGIFLVTEMRKTTRRQIFSSLQQLEQTGVPVLGLVANKAIIKKDSYGYY
                     GKNTASETERY"
BASE COUNT         1107 a          487 c          663 g          971 t
ORIGIN      
        1 aaagaattag caaattattc tggtaaaaag aaatgccgcg ctatggaaaa atgatttgtt
       61 ttgtgctaaa atagattgaa actaatactc aatgaaaatc aaagagtaac ttggaagtta
      121 gctcaaagta cagctttaag gtggtagata gaactgacga agtcagctca aaatactgtt
      181 ttgaggttgc agatggacgc tgacgtggtt tgaagagatt ttcgaagagt ataagatcct
      241 ctcttttgct atagttaagg agatgagagg taaatgataa gggagagact atgtacgact
      301 atctaatcgt tggtgctggt ttgtcgggag caatttttgc ttatgaagca accaagcgtg
      361 gaaaaaaagt aaaagttatt gataaacgta accacattgg tggaaatatc tactgtgaga
      421 atatagaagg agttaatgtt cataaatatg gtgcacatat cttccatact tctaataaga
      481 aagtctggga ttatgtgaat caatttgctg aatttaacaa ctacatcaac tcgcctgtag
      541 ctaattacaa gggtagcctt tataatctac ctttcaatat gaatactttc tatgctatgt
      601 gggggacaaa aactcctcaa gaagtgaaaa ataagattgc tgagcaaacg gctcacatga
      661 aagacgttga accgaaaaac ttggaagaac aggctatcaa gttgatcggt ccagatattt
      721 atgaaaagtt gattaaagga tatactgaaa agcaatgggg acgttccgcg actgaccttc
      781 caccgtttat catcaaacgt ctaccagttc gtttgacttt tgataataac tactttaatg
      841 accgttacca aggtattcca atcggtggct ataatgtcat tatcgaaaat atgctgaaag
      901 atgttgaagt agaacttgga gttgactttt ttgccaatcg tcaggaatta gaagcttctg
      961 ctgaaaaagt tgtctttaca gggatgattg accaatattt cgattataaa catggtgagc
     1021 tagaataccg tagtcttcgt tttgagcatg aagttctaga taaggaaaat tatcaaggaa
     1081 atgccgttgt taattataca gaacgagaaa ttccttatac tcgcattatt gagcacaaac
     1141 attttgagta cggaacacaa gataaaacgg ttattactcg tgaataccca gctgattgga
     1201 aacgtggaga tgaaccttat tatccaatca atgatgagag aaacaatgcc atgtttgcta
     1261 aatatcaaga agaagcagta cagaatgata aggtaatttt ctgtggacgt ttggctgatt
     1321 ataaatacta cgacatgcat gtggttattg aacgggcttt ggaagtggta aggaatgagt
     1381 tctcatgaga gaaacaggca tatatgaaag ttaatgtaga ggagttgagc tttttatttg
     1441 aatattgggt gaaaaagtag tatacttaga tagtctgttt ttaaaaatca aaatggggaa
     1501 atcgattctt atcaacttca gagtgaactg aattaaaact gcataggagt atataaaatg
     1561 ctgaagtaag atttacagaa catggtatga taagtgttcg acagcttgtt tggcaggagt
     1621 tcgaatattc aagtaattga gagggttttt gaacttctga taagatagat agtagattac
     1681 tttcgagtaa aggatagttg agaaaatgaa taatcgtata gtagacatca gtttgtttga
     1741 tttattgaat gtcttatggc ttcgtaaatg gatcattgtt tttgtatcat ttgtttttgg
     1801 atgtatttct tttttatatt atcaattttt tgtacccaaa acctatacaa gtgatactcg
     1861 tatatatgtg gtaagcaaag gggataataa aaatctgtct aatgcagatt tgcaagcagg
     1921 aagtgctcta acaaaggatt acaaagaaat cattttatca gatgaagtgt tagaagaaac
     1981 aatttctgat ttaaaactag aatcaacttt ggaatctcta gcttcgaaaa ttaaaatttc
     2041 tatccctgcg gaaactcgaa ttatttccat tagtgttaca aatacgaact cagatgaagc
     2101 tgctcgaata gcaaatggga taaggaaggt agctgccctt aaaatcaagg aagtaactca
     2161 agttacagat gtcaccactt tacagactgc acgccctccc caaactccgt caggaccgca
     2221 tgtgcgaaaa tctactacag cagggcttgt tcttggtgca ttcttaacag tctttctagt
     2281 agttgccaag gaaatattag atgatcgcat aaaacgtttt gaagaacttg aaaaattggg
     2341 aatccctatt ttaggctcaa tccctttatc aaaaaatata aagtagaaaa tcatgggaaa
     2401 attagaacta acacgtaagt actatcaatt gtacaatgaa acgaaagaat attttaatgc
     2461 cttacagacc aatattcaac ttagtggtga tggactgaaa gtcatttctg ttacttccac
     2521 agtaaaaaac gaaggcaaat cgactatatc agttggcttg tccatgtcct tggccttatc
     2581 aggctataaa gtcctgttac tggatgtgga tttacgtaat tcagtgatgt caggaatgtt
     2641 tcattctagg gaaagaataa caggtttgac tgaatattta tctggcaaag cgggaatggc
     2701 ggattctatg caggaaacta ctttagataa tctctatata ttacaagcag gagtcgtttc
     2761 accgaatccc ttatctctat taaggagtgt taagtttgaa accttgatag attctatgaa
     2821 aaagtacttt gactatatta tagtagatac tccaccaatc ggccaagtaa tagatgctgc
     2881 aattattgcc caacaatgtg atgggatatt tttggtcact gaaatgagga aaacgacacg
     2941 cagacaaatt ttttcttccc tccagcaatt ggaacaaact ggagttcccg ttttgggcct
     3001 tgtggccaat aaagcaatta taaaaaaaga tagctatggc tattatggaa aaaacaccgc
     3061 gagcgagact gaaagatatt gatggactac ctcctctacc tcacaaattg cattttttgt
     3121 gaggtagagg ggtaagttga tactattata tattagactt cctgcgaaac aaaatatggt
     3181 atagtagttc tatgaatgat gaagcaagta aacaactaac tgatgcac
//
LOCUS       DAVDWM010000083         3105 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVDWM010000083 DAVDWM010000000
VERSION     DAVDWM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.083"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..99
                     /locus_tag="VUW49_002083"
     CDS             <1..99
                     /locus_tag="VUW49_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET7981938.1"
                     /translation="FKIFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            180..896
                     /locus_tag="VUW49_002084"
     CDS             180..896
                     /locus_tag="VUW49_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7981939.1"
                     /translation="MKKRYLVVTALLALSLAACSQEKAKNEDGVAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYID"
     gene            complement(1010..1084)
                     /locus_tag="VUW49_002085"
                     /pseudo
     CDS             complement(1010..1084)
                     /locus_tag="VUW49_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            1216..1641
                     /gene="rplK"
                     /locus_tag="VUW49_002086"
     CDS             1216..1641
                     /gene="rplK"
                     /locus_tag="VUW49_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET7981940.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1849..2538
                     /gene="rplA"
                     /locus_tag="VUW49_002087"
     CDS             1849..2538
                     /gene="rplA"
                     /locus_tag="VUW49_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET7981941.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            2916..3074
                     /locus_tag="VUW49_002088"
                     /pseudo
     CDS             2916..3074
                     /locus_tag="VUW49_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1031 a          578 c          684 g          812 t
ORIGIN      
        1 tttaaaatat tttcaacaac ctatcgaaat catcgtaaac gcttcggatt acgaatgaat
       61 ttgagtgctg gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctaataaa
      121 agattagtca aacgtttatg atcagaagta gataaactat tagaaataaa ggagtagaaa
      181 tgaaaaaaag ataccttgtc gtgacagcct tgctagcctt gagtctagca gcttgttcac
      241 aagaaaaagc aaaaaatgaa gatggagtag ctaagacaga acaaacagct aaagctgatg
      301 gaacagtcgg aagtaagtct caaggggctg cccagaaaaa agcagaagtg gttaataaag
      361 gagactacta cagcattcaa gggaaatacg atgaaatcat cgtagccaac aaacactatc
      421 ccttgtcaaa agactacaat ccaggggaaa atccaacagc caaggctgag ttggtcaaac
      481 tcatcaaagc catgcaagag gcaggtttcc caattagtga ccattacagt ggctttagaa
      541 gttatgaaac tcagaccaag ctctaccaag attatgtcaa tcaagatggg aaagaagctg
      601 ctgaccgtta ttctgctcga cctggttata gcgaacacca gacaggcttg gcctttgatg
      661 tgattgggac tgatggtgat ttggtgacag aagaaaaagc agcccaatgg cttttggacc
      721 atgcagctga ttatggcttt gttgtccgtt atctcaaagg caaggaaaag gaaacaggtt
      781 atatggctga agaatggcac ctgcgttatg tcggaaaaga agcgaaagaa attgctgaga
      841 gtggtctcag tttggaagaa tactatggat ttgaaggcgg agattacatc gattaatact
      901 cttcgaaaat ctcttcaaac cacgtcagct tcgtcttgcc gcagttatgg ttactgactt
      961 cgtcagttct gtccacaacc tcaaaacagt gttttgagct gactttgtca gttttatctg
     1021 caacctcaaa gctgtacttt gagcagcctg cggctagctt cctagtttgc tctttgattt
     1081 tcattgagta taaaaaataa acttttctct tgcaattcca gataaatagt gtataataga
     1141 tgggtatgtg aaaaacatac ttgtgggagg taaaaatctc taattaccgc caaaaccaca
     1201 aaggaggatt taaaaatggc taaaaaagtc gaaaaacttg taaaattgca aatccctgct
     1261 ggtaaagcta caccagctcc accggttgga cctgctcttg gtcaagctgg tatcaacatc
     1321 atgggattca caaaagagtt caacgctcgt acagctgacc aagctggtat gatcattcca
     1381 gttgttatct cagtttacga agataaatca tttactttca tcactaaaac accaccagct
     1441 gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taacaaaact
     1501 aaagttgcta cagttactcg tgcacaagta caagaaattg cagaaactaa gatgccagat
     1561 ttgaacgcag caaacgtaga gtctgcaatg cgtatgatcg aaggtactgc tcgttctatg
     1621 ggattcactg ttgttgacta atcaataaca cagtagaaaa tctcacagca gtctattagt
     1681 tgcttttcat actaggcaag tgactgaagc ttgtacttgg gtacagcaag ggagcttaac
     1741 gaagtaggaa aagaaaaata atggactgaa aacccgcaag acttcatcat ttcgagaagt
     1801 gacgtgggag atgaaaatcg attgaaccac ttacaaggag aatagaaaat ggctaaaaaa
     1861 agcaaacaac ttcgtgctgc tcttgagaaa atcgacagca caaaagcata cagcgtagaa
     1921 gaagctgtag cacttgcaaa agaaactaac tttgcaaaat ttgacgcaac tgtagaagtt
     1981 gcttacaact tgaacatcga cgttaaaaaa gctgaccaac aaatccgtgg agcaatggta
     2041 ttgccaaacg gtactggtaa aacttcacgt gttcttgttt tcgcacgtgg tgcaaaagct
     2101 gaagaagcaa aagctgctgg tgcagacttt gttggtgaag atgaccttgt tgctaaaatc
     2161 aacgacggtt ggttggactt cgacgtagtt atcgctacac ctgatatgat ggctcttgtt
     2221 ggacgtcttg gacgtgtcct tggaccacgt aacttgatgc caaaccctaa aactggtact
     2281 gtaacaatgg atgttgctaa agcagttgaa gagtctaaag gtggtaaaat cacttaccgt
     2341 gctgaccgtg caggtaacgt tcaagcaatc atcggtaaag tatcatttga agctgaaaaa
     2401 ttggttgaaa actttaaagc tttcaacgaa acaatccaaa aagcaaaacc agctacagct
     2461 aaaggaactt acgtaacaaa cttgactatc acaactactc aaggtgttgg tatcaaagtt
     2521 gacgtaaact cactttaatc agtagttaaa gtaatgtaaa aaagttgaag acgctatgtc
     2581 tcaacttttt ttgatgtacg acgggcatgt tgtatagtag attgaaacta gaatagtaca
     2641 catctacttc taaaacattg ttagaaattg atttgactgt cctgatcgat tcgtcctgtt
     2701 cttatttcat tttattatat atttgaggtg taagtcctcg gtgggcacct gctaccggtg
     2761 aacccaatag cgattcccaa gtctgactat cgtgaggtag cggattaaaa ctgtctgggg
     2821 atagaccgtt ttaagtctga cgctggaaat aagaatcgtc agaggaaggg atagcgaaat
     2881 cgtggctcta cgaacaggaa cgtgataata aggcctatat agcggataag gtatctggct
     2941 ggggcgacca ctatcaatta gtagctcaga agtcggtact taaacgtgct atatcaaaac
     3001 cagtcctgaa aaaacgtgga ctggtttcgt gtttggatta ttaccttgaa cgacatgcgt
     3061 taaaagttag ttgaaccgcc gtatgccgaa cggcacgtac ggtgg
//
LOCUS       DAVDWM010000084         2922 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVDWM010000084 DAVDWM010000000
VERSION     DAVDWM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.084"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(63..1004)
                     /locus_tag="VUW49_002089"
     CDS             complement(63..1004)
                     /locus_tag="VUW49_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET7981942.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(982..2745)
                     /gene="aspS"
                     /locus_tag="VUW49_002090"
     CDS             complement(982..2745)
                     /gene="aspS"
                     /locus_tag="VUW49_002090"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7981943.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
BASE COUNT          848 a          687 c          587 g          800 t
ORIGIN      
        1 aacatatata cggcaaggcg acgctgacgt ggtttgaaga gattttcgaa gagtattaat
       61 cactaattat ctatctcaac aaatcttcct agaatatgaa cattttccga gacagagaca
      121 aaggagcttg gatccacttg tgtcataatc tgtttaaatt cattaaactc tgcacgtgta
      181 atgacagtga ttaaaactgc ctttctctcg tgattatagg ttccttctgc atcgtggatc
      241 atggttgctc cgcggtgcaa ttttttatgg attttttcaa ttaccttctc tggatgattt
      301 gtcacaatca tggcctgcat acgcttttgc ttagtaaaga ctgcgtctgt cacacggcta
      361 gagacaaaga tggtaatcat agaataaaga gcgtatttcc aaccaaaggt taaacctgct
      421 atcagcatga tagttccatt taccaagaaa gaaatactac cgacattctt acccgttttc
      481 ttgcgaatag tcaggctgac gatatccgtc ccaccactgg agatattgtt tcgaagagca
      541 aaaccaatcc ccaaacccat aacaacaccc ccaaaaaggg aattgataat gggatcttcc
      601 gtcaaggttg ccacagggac aaactggata aagaaggaac tcatagatac cgtgataaag
      661 gtaaagacgg tgaacttatg gccaatctga taccaagcta agaccatcaa agggaagtta
      721 atggcgtaga agcttagcga aatcggaata tgaaaaccaa accagtgatt actcaaggca
      781 gagataatct gtgccagacc tgttgcacca ctcgaataca catgccctgg ttggaaaaag
      841 aaattaactg ctactgctga taaaaaacca tagaccagag aggccgaaat cttctcatca
      901 tacttttctc gagagatact ttgtaagaca cgtaaaattt ttatctgata agcaaagcgg
      961 cgcagataat agcgccatcg cttaattcgt tttgcttgtt tcatcttctt ctacttgtaa
     1021 gctgagttcc tctagttgtt tgagagcgac tgttgatgga gcttgtgtca ttgggtcagt
     1081 tgccttgttg ttcttaggaa aggcaatgac ttcacggata ttttcttctc cagcaagcaa
     1141 catgacaaaa cggtcaagcc cgatagccaa accaccgtgt ggtgggaaac catagtccat
     1201 ggcttcaaga aggaaaccaa actggtcatt ggcttcttca gttgagaaac caagagcctt
     1261 gaacatgcgt tcttgaaggt ctttttggtt gatacgaagg ctaccaccac caagctcata
     1321 accgttcaag acgatatcgt aagcaatggc acgaacctta gccaaatcac cttctaattc
     1381 gtgagcagtc tcttcctgtg gaagggtgaa aggatggtgg gcgctcatgt agcggccttc
     1441 ttcttcagac cattcaaaca tcggccagtc aaccacccaa aggaagttga acttatcatt
     1501 atcaatcaag ccaagctctt tagcaatacg tccacgaagg gcacccagtg ttgcattagc
     1561 cacttcaagc gtatccgcca caaagagaac caagtcctta tcttcaagag caagcgctgt
     1621 tgtcaattct tcttggatac cagtcaagaa cttggcaact ggtccgttta attctccatc
     1681 aaccaccttg acccaagcaa gacctttggc accatactgt ttggctactt ccgtcatctt
     1741 gtcgatgtct ttacgtgaat agttgtccgc agctcctttg accacaatcg cttttacagc
     1801 aggtgcttct gaaaagactt taaagtctac acctttgacc acttctgtca agtcctgaag
     1861 caacatgtca aaacgagtat ctggcttgtc agaaccgtaa agagccatag catcatcgta
     1921 tttcatacga gggaacggta gcgttacttc gatgcctttt gtttccttca tcacgcgcgc
     1981 gatcaagcct tctgtaatat cttggatttc ttgctcagta aggaaggacg tttccaagtc
     2041 gacctgagta aattcaggct ggcggtctcc acgcaagtcc tcgtcacgga aacatttaac
     2101 gatttggtag taacggtcaa aaccagcatt catcaagagc tgtttcgtga tttgtggact
     2161 ttgaggaaga gcgtaaaaat gccccttatt aacacgagac ggtactaaat aatcacgcgc
     2221 cccttcaggc gttgacttag aaaggaatgg tgtctccacg tcgataaact ccaactcatc
     2281 caaatagttg cggatagagt gggtcacctt ggcacgaagt ttaagatttt ccaacatttc
     2341 tggacgacga aggtcaaggt aacggtaacg caaacgtgta tcgtcatttg cctcaatgcc
     2401 atccttaatc tcaaatggtg ttgtcttagc tgtgttaagc acaataagag ctgtcacgtt
     2461 taactcaacc gcaccagttg gcaacttatc attggcttgc tcacgcgcag cgacctgacc
     2521 agtcacctca ataacaaatt cgctacgaag gctttcagct gttgccataa cctctgcaga
     2581 tactttttca gggttgataa ccaactgcat gattccttca cggtcacgaa gatcgataaa
     2641 gatcaaacca ccaaggtcac gacgacggcc aacccatcct ttcaaggtta tttcttgtcc
     2701 gatgtgttcc tcacgaacac gaccagcata catactacgt ttcattattt ctctcctctt
     2761 ttattctgtt actattttac cataaaagcg caggctcttc atgaaaatca tcagaaaagt
     2821 ttgccagtct ttaaaagtca ggtgaaagcc ctaaaaatta gcgctaatac tcttcgaaaa
     2881 tctcttcaaa ccacgtcagc gtcgccttac cgtagtacgg tt
//
LOCUS       DAVDWM010000085         2876 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVDWM010000085 DAVDWM010000000
VERSION     DAVDWM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.085"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..105
                     /locus_tag="VUW49_002091"
     CDS             <1..105
                     /locus_tag="VUW49_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7981944.1"
                     /translation="LPNTGTEDHSSLAALGLLGLMSGFGLVACKKKED"
     gene            167..349
                     /locus_tag="VUW49_002092"
                     /pseudo
     CDS             167..349
                     /locus_tag="VUW49_002092"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            425..640
                     /locus_tag="VUW49_002093"
     CDS             425..640
                     /locus_tag="VUW49_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HET7981945.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLDAYLPWT
                     KVVQEKCKQEISRLGTIREFSSLEIFQ"
     gene            741..1487
                     /locus_tag="VUW49_002094"
     CDS             741..1487
                     /locus_tag="VUW49_002094"
                     /inference="COORDINATES: protein motif:HMM:NF012162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester-forming surface-anchored protein"
                     /protein_id="HET7981946.1"
                     /translation="MKRKLSKQFSAYLSLLILLLGQFLGLSSVKTFADTVDSKRYIGW
                     TITTGETPASFDESIYVSPQGSNAGDLAEAIVAYCFNYTYATPPKLDGTINYPIYEKE
                     TGTAESFGKYATSKHITNKELYTAVLKVMYNGFPNNAASLQGVISDARFRAVTQYAVG
                     HFTDNYDATTILSSDELELYNTLIGIKDGAKNVPEDATLDIYRNTMEVHASTTQGASK
                     PEYQNLLSSRFVNKVTREELKVTPSIDIVE"
     gene            1620..2720
                     /locus_tag="VUW49_002095"
     CDS             1620..2720
                     /locus_tag="VUW49_002095"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017548.1,HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HET7981947.1"
                     /translation="MKKPDVSFRLKNKATDEQVGEILTVSTAESTEESDVIKWENIPR
                     KYSDYIVEEVSVPEGYTSSGRGSGKGTLANPFVITNKKQSDIPAYVQSISVTKVWKGD
                     EADTSKRPDVYFTIFQVKNDIETILTADKLPTPKGFENPKKLTSSAPITWRNLYAPGI
                     DNDTNEPISYIVKETDKDGNVLTSPNYEVSSTVIGANTANHTYTFTNTFKEEKMNSKE
                     IENSKVNLGGEELEGAKIRISKDSKEVESWTSTKTAKSLKLEAGDYVFHEEVAPAGYL
                     AVTDITFTVNEDGTVTVKAANENTVVSTGNKLTVTDQAEESVSSTSEKLKNTDKGRKT
                     LPQTGEITDTWLVYIGITVLFLIISAKFYNKK"
BASE COUNT         1018 a          448 c          571 g          839 t
ORIGIN      
        1 ttgccaaata ccggtactga agatcatagt agtttagcag cacttggact tcttggacta
       61 atgagtggat ttggacttgt agcttgtaag aaaaaagaag attaaaatct ttaaaaatct
      121 aaaggaagat atgtttaagt ttcttaactc aaatttttaa actgaaagga agttttttga
      181 agcgcccccc aagcaagcgg ataaatcatc gttaggagct aaaggtttag cttattgtga
      241 ccagttattt gccttggaaa gagactggga ggctttgcca gctgatgaac gactacagaa
      301 acgtcaagaa catctccaac ccctaaaaga gtccagtgga ctcttttagc ctaagctcag
      361 tttaaaaaag cgagggtggt tgttttctca aaattttgaa ggagctaaag caagagctat
      421 tattatgagt ttgttggaaa cagctaaacg tcatcaatta aatagcgaga aatatctatc
      481 ctatcttcta gaatgtcttc caaacgagga aactctcgta aacaaagagg ttttagatgc
      541 ctatttacca tggactaaag ttgtacaaga aaagtgcaaa caagaaatct ccagattagg
      601 aactatccgt gagttctcta gtctggagat ttttcaatag actttgttat tgggcagtta
      661 cgaaaattca cttccaattg tcaagtcaag tgcaacaaag ttgtttctct gattaagtga
      721 gtatctttca agagtatgaa atgaaaagaa aattatcaaa acaattctca gcatatctaa
      781 gtttactaat tttactctta ggacagtttt tggggttatc ttctgtaaaa acatttgcag
      841 atacggtaga cagtaaacgt tacattggat ggacaattac aactggggag acaccagcat
      901 catttgatga gtctatctac gtaagtcctc aaggaagcaa tgccggagat ttagctgaag
      961 caattgttgc atattgcttt aactatacct atgcaacccc acctaagtta gatggtacca
     1021 taaactatcc tatttacgaa aaagagactg gaacagcaga aagttttggg aaatacgcca
     1081 cctctaagca tattacaaat aaagagttgt atacagcagt attaaaagtt atgtataatg
     1141 gtttcccaaa taatgcagcg agtctacaag gggttattag tgatgcacgt tttagagctg
     1201 taactcagta tgctgtgggg cattttaccg ataattatga tgcaacaacg atacttagca
     1261 gtgatgaatt agagctttat aatacattaa ttggtataaa agacggtgca aaaaatgtac
     1321 ctgaagatgc tacattagac atttatcgta acacaatgga ggttcatgct agtactacac
     1381 aaggagcttc aaaaccagaa tatcaaaatc ttttatcttc tagatttgta aataaagtaa
     1441 ctagggaaga gcttaaagtt actccatcta ttgatatagt ggaatgaaat aagatatgaa
     1501 caaattgatt aggaaaatca aatgaattta tagaaatatt ttagcagtca tggcgtccta
     1561 ttctagtttc aaaatgatat atttatgcta agaaagtttg ggaaaatgct aattctattg
     1621 tgaaaaaacc tgatgtttca ttccgtttga aaaataaagc aacagatgag caggtaggtg
     1681 agattttaac agtttctact gctgaaagta cggaagaatc agatgttatc aaatgggaaa
     1741 atattcccag aaagtactca gactatattg tggaagaagt atcagtacct gagggatata
     1801 catcatctgg acgtggttct gggaagggga ccttggctaa tccttttgtg attacaaata
     1861 aaaaacaatc agatattcct gcttatgtcc aatctatatc tgttacaaag gtttggaaag
     1921 gagatgaggc tgatacttcc aaacgtcctg atgtttattt tactattttt caagtaaaaa
     1981 atgacattga gacaattttg actgctgaca aattaccgac tccaaaagga tttgaaaatc
     2041 ctaagaagtt aacttcatca gcacctataa catggcgaaa tctgtatgca cctggaattg
     2101 ataatgatac aaatgaaccg atttcttata ttgtgaaaga gacagataaa gatgggaatg
     2161 tcttaacatc acctaactat gaagtgtctt ctacagtgat tggtgcaaat acggctaatc
     2221 atacctatac ctttaccaat acattcaaag aagagaagat gaatagcaaa gagattgaga
     2281 acagtaaagt caatcttgga ggcgaagagc ttgaaggagc taagattcgt atttcaaaag
     2341 atagtaagga agttgaaagc tggacttcta ctaagactgc taagtcactg aaactagaag
     2401 caggagacta tgtcttccat gaggaagtag caccagctgg atacctggca gttactgaca
     2461 ttacgtttac tgttaatgaa gatggtacag tgactgtcaa agccgctaat gaaaatacgg
     2521 ttgtgtcaac tggtaataag cttacggtaa ctgaccaagc cgaggaaagt gtttcttcaa
     2581 catcggaaaa attaaaaaat acagataagg gaaggaaaac tctacctcaa actggtgaaa
     2641 ttacagatac ctggcttgta tacattggca ttacagtatt attccttatt ataagtgcta
     2701 agttttacaa caaaaaataa atatcaatta tagcggattg ataaaaacct aatattaagt
     2761 gctctttgtc aactgtagtg ggttgatgaa aagttaggct ctataatatt tgtagtgggt
     2821 aaattcccta tggatattat ggagcctatt ttgtgtagaa aaaaagtccc ataaga
//
LOCUS       DAVDWM010000086         2699 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVDWM010000086 DAVDWM010000000
VERSION     DAVDWM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.086"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            1..500
                     /locus_tag="VUW49_002096"
                     /pseudo
     CDS             1..500
                     /locus_tag="VUW49_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(557..1834)
                     /gene="ltrA"
                     /locus_tag="VUW49_002097"
     CDS             complement(557..1834)
                     /gene="ltrA"
                     /locus_tag="VUW49_002097"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET7981948.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSDKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPGGGIRQLGIPTVMDRMIQQTIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQLYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
BASE COUNT          751 a          603 c          476 g          869 t
ORIGIN      
        1 agctatggac tacactcgaa aaaaagaacc acacctacta tgaacaagac ccagaaaaag
       61 tagccttatt tcttaagaat tttaatagtt taaagcacct agcacctgtt tagattgatg
      121 aaacaggatt cgatacttat ttttatcgag aatatggtcg ctcattaaaa ggtcagttaa
      181 gaagaggcaa agtatctgga agaagatatc agaggatttc tttggttgca ggtctaacaa
      241 atggtgagtt aatcgctcca atgacttacg aagagatgat gacgagcgac ttttttgaag
      301 cttggtttca gaagtttctc ttaccaacat taaccacacc atcggttatt attatggata
      361 atgcaagatt ccatagaatg gggaagctag aacttttatg caaagagttt gggcataaac
      421 ttttacctct tcctccctac tcacctgagt acaatcctat tgagaaaaca tgggctcata
      481 tcaaaaagca catagagtag gggataatct ctagcccctc tcacaccacc gtacgtgccg
      541 ttcggcatac ggcggttcaa ctaactttta acgcatgtcg ttcaaggtaa taatccaaac
      601 acgaaaccag tccacgtttt tccaggactg gttttgatat agcacgttta agtaccgact
      661 tctgagctac taattgataa tggtcgcccc agccagatac cttatctgct atccatttag
      721 gaacccctaa cttaagcaat ccccataatc gtctcgattt cttcttccat tgcttccaga
      781 taatcactcg taggcgagta cgcaagcgct catctatgct ggcgactata cttttcatat
      841 ttcccaatga gaaatagttt atccatcctc gaatagacaa attcagttgc tcaatacgtc
      901 ttgttaggtc tatactccat ttcctctgtg ttagtttctt caatttaaac ttaaatctcc
      961 gaacactatc ttgatgtgga cggcttttcc aaccatctga tgatttccag aacccaaaac
     1021 ctagatattt caactctctt ggtctggtaa tcttagtctt ggtcatgttt actttcaaac
     1081 ctagccgttt ctcaataaaa cgactgactg aatacatcac acgcttagag gctgcctcgc
     1141 ttccgaccgt aatcacacaa tcatctgcgt agcgcacaaa tcgaagtccc ctcttttcta
     1201 attccttgtc caattcatta agcatgatat tggataagag aggagataaa tttcctccct
     1261 gtggtgtacc aactagtgtt ttataaagtt gaccattaat gataacacct gaatgaagat
     1321 acttacgaat caaggattcc gtatctccgt cttcgataat gttatgtact aaggacatca
     1381 atctatcttg aggaactgta tcgaaaaatt tctctaggtc tatatccact atccactcat
     1441 agccgtcatt taagtattct aagagcttca tgatggcttt ttcacatgac ctatttggtc
     1501 tgaaaccata actcgtatct gagaaatggg gttcacaaat ggggctcatg acttggacaa
     1561 tggtctgttg aatcattcta tccataactg ttggaattcc tagttgacgg atacctccgc
     1621 ctggtttagg tatctcaact ctaagaactg gttgaggctt atattttctc tgttttatca
     1681 gttccttagt caagcgccag ttttgtctga gataattatc catctcttcg atagtcattc
     1741 catcaatccc agctgagcct ttatcggatt ttacttgatt gtaggcttcc agcatatttt
     1801 cgcgtgataa tattttatct agcaattttg acatgtgcct attcctttct tgttttggtg
     1861 cctgagggac atcttatatt ggtgaacctt catctagtca ttacgtgtca aagtccagtt
     1921 ctatcagacc actcatttta cagacacaag tgaagtagga ttacttcgat tagttgttca
     1981 ccccttcgct ccacttccat tacggaagtt tcctcactac tatgggctcg gctgacttct
     2041 catgattcgt tgttactacg ctttcagcgc tcatgaaacc tcacgggata agtcgtacat
     2101 ctttcctcgt ttatacaaga tatgaggacg ttaaaaggtt acagtgaaaa taggaaatct
     2161 gacgcagagt ctttagactc tagaaagatt tatctttttc acacaaacct tagtccgaat
     2221 tcaattactc tcgtgattta cgcatatagg ttacgactac ctttttggac tttagagttt
     2281 gaggccccct tatccgctat atacgcctta ctatcacgtt cctgttcgta gagccacgat
     2341 ttcgctatcc cttcctctcc ctgctacctc acgatagtca ggcttggaaa tcgctattgg
     2401 gttcaccggt agcaggtgcc caccgaggac ttacacctca gatgcacgac atgcccgtcg
     2461 tacatcaaaa aggtattacc aagttgcaat accttttacg aggctctttt gtccggctct
     2521 tgtttcaatt gactataatt tctaacaatg ctttagaaac catagtgttc tattccaaat
     2581 tcaatccatt atattttaga ttttgtgaaa aaaacattga tacaaaaagc aggtgatttt
     2641 ctggctctat aatatttgta gtgggtaaat cccctatgga tattatggag cctattttg
//
LOCUS       DAVDWM010000087         2517 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVDWM010000087 DAVDWM010000000
VERSION     DAVDWM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.087"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..550
                     /locus_tag="VUW49_002098"
     CDS             <1..550
                     /locus_tag="VUW49_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7981949.1"
                     /translation="CEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWS
                     GFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVAD
                     SHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPP
                     LPEQSRIVDKIEQFFAHIDALI"
     gene            607..1404
                     /gene="psrA"
                     /locus_tag="VUW49_002099"
     CDS             607..1404
                     /gene="psrA"
                     /locus_tag="VUW49_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HET7981950.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1415..2020
                     /locus_tag="VUW49_002100"
     CDS             1415..2020
                     /locus_tag="VUW49_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7981951.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1968..>2517)
                     /locus_tag="VUW49_002101"
     CDS             complement(1968..>2517)
                     /locus_tag="VUW49_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7981952.1"
                     /translation="ETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIP
                     WVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATH
                     NEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNH
                     EEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          862 a          361 c          435 g          859 t
ORIGIN      
        1 ttgtgaaata ccagaaagtt gggaatgggt gaggttaaac gatataacct cctacattca
       61 aagagggaag tctccaaaat attcaaatat tcctatatac cctgtgattg ctcagaaatg
      121 taatcaatgg tctggctttt ctattgactt agcaagattt attgatcccg aaacagttca
      181 tagttaccag aaagaaagat tactaagaga tggtgattta atgtggaatt caacaggtct
      241 aggtacgtta ggacgattag ctatttacca tgaaaataaa aatccttatg gctgggctgt
      301 tgcagatagc catgttacag ttataagagt cttatcggga gttattaatt gccatttcat
      361 ctataatttt ctttcctctc ctattgttca gtcagttatt gaagaaaaag cgtcgggtag
      421 tacaaaacaa aaagaattat taacgaaaac tataaaagaa tatttaatcc ccctcccacc
      481 ccttcctgaa caatccagaa tcgttgataa aatcgaacaa tttttcgccc atattgacgc
      541 actaatttag tataatagtt ttacgtgcgg tggaatttct atcaaacata agataggagt
      601 tttacgttga aagattttga attacatttg aagaaagctg gtttagctga aaatactgtg
      661 cgttcgtacc tttatggtgt tcgatttttc ttggagaatt acgaactcaa aatggaagat
      721 ttatttgaat acaaaggata tttattagac aattttaaac ctaaaactgt caatttgcga
      781 ctacaaggag taaataaata tctggctttt attggtcatg atgatttgaa attaaaattt
      841 gtcaaagtac agcaaaaacc gtttttagaa gatgtcatta gccatgctga ttaccttttt
      901 cttaaacgta gtttgaaaaa agatggtatt ttaaaatggc attttgtcgt ttggttttta
      961 ggtgcaactg gggctcgtgt aagtgaactt attaaactaa aagtagagca tgtagaaatc
     1021 ggttattttg acatttactc aaaaggtggt aaaattcgta gactgtacat tcctaaaaaa
     1081 ttaagaaata gttgtcttag ttggttagaa tcagaaaatc gccgaagtgg ttatttattt
     1141 ttgaataaat ttaacgaacc aattacagca agaggagttg ctcaacagtt aaaaaattat
     1201 gctgataaat acaaaatgaa tcctaaagta atttaccctc attcttttag gcatttattt
     1261 gctaagaatt ttttagcgaa gtataatgat attgccttgc ttgcagattt gatgggacac
     1321 gaaagtatag aaactactcg aatttatcta aggaaaacag ctactgaaca acaaaatatt
     1381 gtagataaaa ttgttaattg gtaaaaaata acaggtggtc aaactgacta cctgctattt
     1441 ttgtgattat ggctcttatt atgggaatat acctatgaat tgggttgtta taaaaataaa
     1501 agatattttt tcaatgaata caggtctttc ttacaagaag ggcgatttaa gcattaataa
     1561 taaaggtgtt agaattatac gtggtggtaa tattaagcct ttagaatttt ctctgttgga
     1621 taacgattac tacattgata cacaattcat ctcctctgag caagtttatt taaaacataa
     1681 tcagctaata acacctgtat caacctcttt agaacatatt ggaaagtttg caagaatcga
     1741 taaagactat gatggtgttg tggctggtgg atttattttc caattaacac cattcgaaag
     1801 ttcagagatt atttcaaaat ttctattatt taacttgtcc tctccgttat tttataaaca
     1861 attgaaagca ataactaaac tatcaggtca agctttatat aatattccta aaactacact
     1921 gagcgagcta ttaattccgt tagctccttt tgaggaacag gaacttatta ctcaaaaagt
     1981 tgagaaactt tttgaaaaag taaatcaact ttggaaatga ttcttttcat ctcttcatga
     2041 ttagaaatag ggattaataa ttcggagata ctggtactat ttaatgtttt ccctttgata
     2101 gcatcttttg aatcacctaa agtagagata agtggcaaaa atatcattaa gtaatctctg
     2161 ataatatttt ctttattagc ataggggaat atcgatataa tggcttcatt atgagtggca
     2221 ggaatatcca atatggcaac ttttccaata gataatttaa aactcattaa taaagttcct
     2281 ttaggtgaaa tgtctatttt ctttgatttt aatgctaatt tagaaataga ttctctcgta
     2341 ttagttacat aaccagatat aggcatatct gatatagata cccaaggtat ttcagttccc
     2401 caaaaagtag cttcactgcg tggaggagtt tttcctattc tgaagttaac taggctagca
     2461 aatttaatat atctccatgc ttctgggatt tcatatatag gataagaggt tgtttcg
//
LOCUS       DAVDWM010000088         2406 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVDWM010000088 DAVDWM010000000
VERSION     DAVDWM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.088"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..160)
                     /locus_tag="VUW49_002102"
     CDS             complement(<1..160)
                     /locus_tag="VUW49_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7981953.1"
                     /translation="MAYSIDFRKKVLSYCERTSSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVK"
     gene            242..693
                     /locus_tag="VUW49_002103"
                     /pseudo
     CDS             242..693
                     /locus_tag="VUW49_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            858..1157
                     /locus_tag="VUW49_002104"
     CDS             858..1157
                     /locus_tag="VUW49_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET7981954.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1266..2406)
                     /locus_tag="VUW49_002105"
                     /pseudo
     CDS             complement(1266..2406)
                     /locus_tag="VUW49_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          717 a          465 c          423 g          801 t
ORIGIN      
        1 cttttacttg gtggtttagc tctcctgttt tctcttttag ctttaaccag ccataaatgg
       61 tattacgtga gatttggaaa acgtgtgatg cttctgttat actacttgtt cgctcacaat
      121 aagagagaac ttttttacga aaatctattg aatatgccat aaaaagatta taccacattg
      181 tgtactattt ttggttcatt tcactataat aataacgata ctagataatg aggaaaatgc
      241 aatggcaaac ccaactttcg gagaaaaaaa gtgaatacag actatattgc tcgctatggc
      301 gtatatgcag ttatccctaa tcctgaacaa aaacaaattg ttcttgttca agaaccaaat
      361 ggtgcttggt tcctaccatg tggaaaaatt gaagcaggtg aaaatcatca ggaagcccta
      421 aagcgtgagt tgattgaaga gcttggtttc acagcagaaa ttggtaccta ttacggacaa
      481 gctgacgaat atttctattc tcgtcatcgt gatacctact actacaatcc tgcctacctc
      541 tatgaagcaa ctcctttcaa agaagtacaa aagccactag aaaactttaa tcatattgcc
      601 tggttcccta ttgacgaggc tatcaaaaac cttaaacgtg gtagccataa atgggccatt
      661 gaatcttgga aaaaacagca taagattggc taatactctt cgaaaataaa attcaaatca
      721 cgtaagcgtc gccttaccgt actcaagtat agcctgcggc tagcttccta gtttgctctt
      781 tgattttcat tgagtataaa tcaagcgact ttattcaaaa agtgttataa tagaattaga
      841 aaatcggagg aaatattatg aagcttatca atacgacaaa ctcacactcg caacttgtaa
      901 aaagtcaact agaaagtacc gatgcaaccc ttgttgaggt ttattctgca gggaatacag
      961 atgttatttt tacccaagct ccgcttcact atgaaatcct catctcaaac aaacaccgtg
     1021 ctattcgtga gcctgaaatc gaaaccattc aagaattttt cttaaaacgt aaaattgata
     1081 agacctctgt tgatgaagcc aatatcaaga cactctactc agagaaatta attggaattt
     1141 caatcccaat caagtaagaa aattgagtaa aaactcgatt tgagagaaag tccaataagt
     1201 ctttccataa caaaacgcat agtagcaaga gttttgtact gaccccaaaa agttagacaa
     1261 ttaatttatc cgaaggattt agttctgtat tgcacagggc taagtccttt tagttttacc
     1321 ttaattcgtt tgttgttgta gtaatcaata tagtctataa tggcttgttc caattggtta
     1381 agtgatttaa atgttttctc atagccataa aacatttcgg attttaaaat accaaagaag
     1441 gactccatca taccattgtc ttggctgttt cccttgcgtg acatagatgc ttgaattccc
     1501 ttactctcta ggaaccgatg ataagaatcg tgttgatatt gccagccttg gtcactatgg
     1561 agaatcgtat tctcgtagtg cttctctgtg aatgcctgtt ccaacattgt ttgtacttgt
     1621 tctaagttgg gtgaagttga aagattatag gcgataattt cgctgttaaa gccatctaaa
     1681 actggtgata agtaaagctt ttgagtactt gctggaatgg caaattcagt cacatctgtg
     1741 tagcactgtt ccattgtttt agagccttca aattggcctt gaatgagatt ctctgctttc
     1801 ttgccaacgt ctcctttatg agaagaatat tttcgtttct gtcgcgtttt agcttgtaaa
     1861 ttgagtactt catcaagtgt tgaactcttt tatgatttac cagataacca cgatttctta
     1921 gttctaaatg aacccggcga taagcataat ttcccttgtg ttcgataaaa atggattgaa
     1981 tttcagcttt aagctcttgg tccgtatctg gtttatctag ctgtttcaag tgatagtagt
     2041 aggtccaacg agctagttta atggctttta gaagaagaac taacgaaaac tcagtcatta
     2101 attcttgaac aatttctgtc tttcttcttt ctctttttcc tccttcaatc ggagttctct
     2161 taactttttt aggatggcat tctccgctct caggtactca ttttctgctt gaagacgttc
     2221 taattctgtc ctctcttcag gtctcttttt tggcttacgt cccattttag gtactctccc
     2281 tcttgttttc tcaacaatag tatacccgtt tttcctgtat tgtgctagcc agttaagaag
     2341 tatcgtacga cttgggagac cgtattcaag agaaactcta tctttagtcc agccttcatg
     2401 tcagac
//
LOCUS       DAVDWM010000089         2053 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVDWM010000089 DAVDWM010000000
VERSION     DAVDWM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.089"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..207
                     /locus_tag="VUW49_002106"
     rRNA            <1..207
                     /locus_tag="VUW49_002106"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            259..331
                     /locus_tag="VUW49_002107"
     tRNA            259..331
                     /locus_tag="VUW49_002107"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:292..294,aa:Ala,seq:tgc)
     gene            456..>2053
                     /locus_tag="VUW49_002108"
     rRNA            456..>2053
                     /locus_tag="VUW49_002108"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          604 a          396 c          604 g          449 t
ORIGIN      
        1 cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac
       61 acaccgcccg tcacaccacg agagtttgta acacccgaag tcggtgaggt aaccgtaagg
      121 agccagccgc ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc
      181 ggaaggtgcg gctggatcac ctcctttcta aggataagga actgcgcatt ggtcttgttt
      241 agtcttgaga ggtcttgtgg ggccttagct cagctgggag agcgcctgct ttgcacgcag
      301 gaggtcagcg gttcgatccc gctaggctcc attggtgaga gatcaccaag taatgcacat
      361 tgaaaattga atatctatat caaatagtaa caagaaaata aaccgaaaac gctgtagtat
      421 taataagagt ttatgactga aaggtcagaa aataaggtta agttaataag ggcgcacggt
      481 ggatgccttg gcactaggag ccgacgaagg acgtgacaaa cgacgatatg ccttgggtag
      541 ctgtaagtaa gcgatgatcc agggatttcc gaatggggga acccaacagg taatacctgt
      601 tacccacatc tgttaaggat gtgaggagga agacgcagtg aactgaaaca tctaagtagc
      661 tgcaggaaga gaaagcaaaa gcgattgcct tagtagcggc gagcgaaacg gcagaagggc
      721 aaaccgaaga gtttactctt cggggttgta ggactgcaat gtggactcaa agattataga
      781 agaatgattt gggaagatca gccaaagaga gtaatagcct cgtatttaaa atagtctttg
      841 tacctagcag tatcctgagt acggcgggac acgtgaaatc ccgtcggaat ctgggaggac
      901 catctcccaa ccctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      961 gtgaaaagca ccccgggagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
     1021 tcgagcccgt taatgggtga gagcgtgcct tttgtagaat gaaccggcga gttacgttat
     1081 gatgcgaggt taagttgaag agacggagcc gtagggaaac cgagtctgaa tagggcgcct
     1141 tagtatcatg acgtagaccc gaaaccatgt gacctaccca tgagcaggtt gaaggtgcgg
     1201 taagacgcac tggaggaccg aaccagggca cgttgaaaag tgcttggatg acttgtgggt
     1261 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
     1321 tagcgtcgat attagagatt cttggaggta gagcactgtt tgggtgaggg gtccatcccg
     1381 gattaccaat ctcagataaa ctccgaatgc caatgaatta tggtcggcag tcagactgcg
     1441 agtgctaaga tccgtagtcg aaagggaaac agcccagacc accagctaag gtcccaaaat
     1501 aattgttaag tggaaaagga tgtggggttg cacagacaac taggatgtta gcttagaagc
     1561 agctattcat tcaaagagtg cgtaatagct cactagtcga gtgaccctgc gccgaaaatg
     1621 taccggggct aaaacaattt accgaagctg tggatacctt tataggtatg gtaggagagc
     1681 gttctatgtg tgatgaaggt ataccgtgag gagtgctgga acgcatagaa gtgagaatgc
     1741 cggtatgagt agcgaaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg
     1801 ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga
     1861 tggacaacag gttgatattc ctgtactaga gtatgtagtg atggagggac gcagtaggct
     1921 aactaaagca gacgattgga agagtctgtc taagcagtga ggtgtgaatt gagtcaaatg
     1981 cttaattcta taacattgag ctgtgatggg gagcgaagtt tagtagcgaa gttagtgatg
     2041 tcacactgcc aag
//
LOCUS       DAVDWM010000090         2016 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVDWM010000090 DAVDWM010000000
VERSION     DAVDWM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.090"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(74..1549)
                     /locus_tag="VUW49_002109"
     CDS             complement(74..1549)
                     /locus_tag="VUW49_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7981955.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          588 a          456 c          372 g          600 t
ORIGIN      
        1 tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc atcaaattga aataagaaat
       61 agtttagata aaactattga cttttcttat agtttgtctt tctcttaata acgttgatat
      121 gattgacctc tggatcttcc gtcccttgga gggagcaacc acgttccttg gcgaattgga
      181 tatagtctac aatttctcgt gtaatgcgtt caccaggagc caagatagga atccctggag
      241 gatagcacat gacaaattct ccacagacct gtccaacaga ctcatctaag gtcaaacttt
      301 ttctctctga atagaaggct tcttgtggag acagcaccaa ctcgggctga atatattctc
      361 cagctattaa gtccttccca tctcgtgaat agagtctctt aatatcagcc agagcaccaa
      421 ccaagcgctc gatgtcttgg atgcggtcgc cgatggaaat ataggccaag atattgccga
      481 tatcaccaaa ctcgatctga atgtcgtatt cgtctcgcaa gaggtcataa acctcgatac
      541 ctgttaagcc tattccctga gtgtaaactg acaacttggt tacatcaaaa tcacagaccg
      601 acacaccatc tattaactct tttgagtaag catagtaacc gccaatggca ttgatttcat
      661 gacgagcgta ctcggatagc tcaatgactt cctcaaacga ctctttacca cgaagggcca
      721 agttgcgacg tgaaatatcc aaactagcca tcaataagta agaggcagat gtagactgtg
      781 tcaggtttat aatctgacga acgtactcgg gattcatctg ctccccgatt aaaagaagcg
      841 aactttgtgt caaactccca ccagacttat gcatggaaac tgctgccata tcagctcctg
      901 catccatagc agaaattgga agtttaccag tgaaatgcaa atgcgctcca tgggcttcat
      961 ctactaaaac catcatgcca gcttcatgag ccatttctgt caaccccttt aggtctgaac
     1021 agatgccgta gtaagtagga ttgttgatta ggatagcctt agcatctgga tggtccttta
     1081 tggcctgtgc tactcggtca ttttcaagac ctaaagcgat accaatctta ggatctacac
     1141 tcatctcgat atagatggga atggcaccac atagaaccaa cgcattgata gcagatttat
     1201 ggacatttcg tggcagaata atcttatctc ctgccttgca ggttgccaga atcatagtct
     1261 gcaccgatga agttgttcca ccaatcatta gaaaggcatg gctagctcca aaagcatctg
     1321 cagccagctc ctctgcatca cgaataatcg aaataggatg gcctaaatta tccaagggtt
     1381 tcatcgaatt gacatcaatg cctacacatt tttctcctaa gagttcgaca agttctggat
     1441 ttccccgtcc acgcttgtga cctggaacat caaagggaac aatccttttc ttgcgtaact
     1501 tcaccaaggc ctcataaatt ggggcttggt tttgatctaa ctctttcaaa acatactcct
     1561 ttcaatattt ttaggacctt atgaaaacta aggaactaaa ggttgactca tgaaaaaaga
     1621 gcaggttttc acctgctctg caatcttctg acgtgtttat tttggtttct gttgagtatg
     1681 tctgttcccg atgtttccta actctctagt agcaaatatt acgtacttta ttgatcgttt
     1741 cacaagatga cgtgtgcttt caccacacta aaaatttatg aattaggaca agtgtcctaa
     1801 catcaactta taaaagtagg cttttaaccc tatcaataat gtggcttttc aaccacgctt
     1861 cggcattcaa ccctgcctgt ccgtaggcat tttttactcg ggtttatatt tgctagaaaa
     1921 catcatctca ttttctaagc tttattacta tatcatgttt ttaagaactt gtaaagtatt
     1981 ttttgataaa aaacaaaaat atagtagatt gaaact
//
LOCUS       DAVDWM010000091         1860 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVDWM010000091 DAVDWM010000000
VERSION     DAVDWM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.091"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(3..968)
                     /locus_tag="VUW49_002110"
     CDS             complement(3..968)
                     /locus_tag="VUW49_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7981956.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VUW49_002111"
     CDS             complement(1086..1499)
                     /locus_tag="VUW49_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET7981957.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VUW49_002112"
     CDS             complement(1496..1732)
                     /locus_tag="VUW49_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET7981958.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          542 a          363 c          275 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagtctc
      241 tgggcttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtccgagca tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctggta ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaacccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga gacttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcagata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DAVDWM010000092         1822 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVDWM010000092 DAVDWM010000000
VERSION     DAVDWM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.092"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            90..1010
                     /gene="cysK"
                     /locus_tag="VUW49_002113"
     CDS             90..1010
                     /gene="cysK"
                     /locus_tag="VUW49_002113"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7981959.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1207..1605)
                     /locus_tag="VUW49_002114"
     CDS             complement(1207..1605)
                     /locus_tag="VUW49_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981960.1"
                     /translation="MKAPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEGWARM
                     GLPITRKEIVNWHIKASQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            1596..1822
                     /locus_tag="VUW49_002115"
                     /pseudo
     CDS             1596..1822
                     /locus_tag="VUW49_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          567 a          345 c          371 g          539 t
ORIGIN      
        1 aaatcctatc gcttcgatag gatttctata ttttacaaat ggtatggata gcgttatact
       61 aaagatatct tatacaaaga ggtattcata tgtctattta taacaacatt actgaattaa
      121 tcggtcaaac accgattgtt aaacttaaca acatcgtgcc agaaggtgct gcagacgtct
      181 atataaagct tgaagcattt aatcctggtt catctgtaaa agaccgtatt gcccttagca
      241 tgattgaaaa agctgaacaa gatggtattc tgaaacctgg ttctactatt gttgaagcaa
      301 caagtggaaa cactggtatt ggactttcat gggtaggtgc tgctaaaggg tataaagtcg
      361 tcatcgttat gcctgaaact atgagtgtag aacgacgtaa aattatccaa gcttatggtg
      421 ctgaactcgt cctaactcct ggtagcgagg gaatgaaagg tgctattgct aaggctcaag
      481 aaatcgctgc tgaacgtgat ggtttccttc ctcttcaatt tgacaatcca gctaatccag
      541 aagtacacga aagaacaaca ggagctgaga tactagctgc tttcggtaaa gatggattag
      601 atgcctttgt tgctggagta ggtaccggtg gaacgatttc tggtgtttct catgcactca
      661 aatcagaaaa ttctaacatt caagtttttg cagtagaagc agatgaatct gctattctat
      721 ctggtgaaaa acctggtcct cacaaaattc aaggtatctc agctggattt attcctgata
      781 cacttgatac taaagcctat gatggtatcg ttcgtgtaac atcagatgac gctcttgcac
      841 tcggacgtga aattggtgga aaagaaggct tccttgtagg gatttcctca gctgcagcta
      901 tctacggagc cattgaggtt gccaaaaaat taggtacagg taaaaaagtc cttgccctag
      961 caccagataa cggtgaacgt tatctctcta cagcacttta tgaattgtaa ccatccaata
     1021 acgaagtata ttgaaaaatc tccagactag agaactcacg gatagttcct aatctggaga
     1081 tttcttattt gcacttttct tgtacaactt tagtccatgg taaataggcc tctaaaacct
     1141 ctttgtttac gagagtttcc acgtttggaa gacattctag aagataggat agatatttct
     1201 cactatttat aatggattga aataagatat gaacaaatcg attaggacat gatggtaaag
     1261 cgtaatccct tgtttctcag ctttcccaga caaaaaagtc caatagtaag tcagctgact
     1321 atcactctct agcaccctat aagaagtttc atccgcatga agtaaggact gcgtcaatag
     1381 tctctctcgc aagaggttat aaaggggctc caaatagtat tgactcgcct tgatatgcca
     1441 attaacaatt tccttacgtg tgattggtaa acccatccta gcccaacctt cttcttggcg
     1501 ataattgggt accttcagat taaacttctg atggatggtg tgagcgataa tagaagctga
     1561 gccaaagtta tgcgctaaag gggctttagg aataggagct ttcacaagct tatccagatg
     1621 attatctttt actcgttatg gataatgcta tatggcataa atcaagtacc ttaaagattc
     1681 cgactaatat tggcttttca tttattcctc catacacacc agagatgaac cccattgaac
     1741 aagtgtggaa agagattcgt aaacgtggat ttaagaataa agcctttcga actttggaag
     1801 atgtcataca aggactggag aa
//
LOCUS       DAVDWM010000093         1708 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVDWM010000093 DAVDWM010000000
VERSION     DAVDWM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.093"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..>1708
                     /gene="phtD"
                     /locus_tag="VUW49_002116"
     CDS             <1..>1708
                     /gene="phtD"
                     /locus_tag="VUW49_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HET7981961.1"
                     /translation="LKDAAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAARAQGRY
                     TTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELAAAEAYWNGKQGSRPS
                     SSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSLLRELYAKPLSERHVESDGLIF
                     DPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQP
                     SPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDL
                     SAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQ
                     VDFEALDNLLERLKDVSSDKVKLVEDILAFLAPIRHPERLGKPNAQITYTDDEIQVAK
                     LAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKG
                     LTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYD
                     HYHNIKFEWFDEGLYEAPKGYSLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNK
                     NG"
BASE COUNT          550 a          371 c          361 g          426 t
ORIGIN      
        1 accttaaaga tgcggcccat gcggacaata ttcggacaaa agaagagatt aaacgtcaga
       61 agcaggaacg cagtcataat cacaactcaa gagcagataa tgctgttgct gcagccagag
      121 cccaaggacg ttatacaacg gatgatgggt atatcttcaa tgcatctgat atcattgagg
      181 acacgggtga tgcttatatc gttcctcacg gcgaccatta ccattacatt cctaagaatg
      241 agttatcagc tagcgagtta gctgctgcag aagcctattg gaatgggaag cagggatctc
      301 gtccttcttc aagttctagt tataatgcaa atccagctca accaagattg tcagagaacc
      361 acaatctgac tgtcactcca acttatcatc aaaatcaagg ggaaaacatt tcaagccttt
      421 tacgtgaatt gtatgctaaa cccttatcag aacgccatgt ggaatctgat ggccttattt
      481 tcgacccagc gcaaatcaca agtcgaaccg ccagaggtgt agctgtccct catggtaacc
      541 attaccactt tatcccttat gaacaaatgt ctgaattgga aaaacgaatt gctcgtatta
      601 ttccccttcg ttatcgttca aaccattggg taccagattc aagaccagaa caaccaagtc
      661 cacaatcgac tccggaacct agtccaagtc tgcaacctgc accaaatcct caaccagctc
      721 caagcaatcc aattgatgag aaattggtca aagaagctgt tcgaaaagta ggcgatggtt
      781 atgtctttga ggagaatgga gtttctcgtt atatcccagc caaggatctt tcagcagaaa
      841 cagcagcagg cattgatagc aaactggcca agcaggaaag tttatctcat aagctaggag
      901 ctaagaaaac tgacctccca tctagtgatc gagaatttta caataaggct tatgacttac
      961 tagcaagaat tcaccaagat ttacttgata ataaaggtcg acaagttgat tttgaggctt
     1021 tggataacct gttggaacga ctcaaggatg tctcaagtga taaagtcaag ttagtggaag
     1081 atattcttgc cttcttagct ccgattcgtc atccagaacg tttaggaaaa ccaaatgcgc
     1141 aaattaccta cactgatgat gagattcaag tagccaagtt ggcaggcaag tacacaacag
     1201 aagacggtta tatctttgat cctcgtgata taaccagtga tgagggggat gcctatgtaa
     1261 ctccacatat gacccatagc cactggatta aaaaagatag tttgtctgaa gctgagagag
     1321 cggcagccca ggcttatgct aaagagaaag gtttgacccc tccttcgaca gaccatcagg
     1381 attcaggaaa tactgaggca aaaggagcag aagctatcta caaccgcgtg aaagcagcta
     1441 agaaggtgcc acttgatcgc atgccttaca atcttcaata tactgtagaa gtcaaaaacg
     1501 gtagtttaat catacctcat tatgaccatt accataacat caaatttgag tggtttgacg
     1561 aaggccttta tgaggcacct aaggggtata gtcttgagga tcttttggcg actgtcaagt
     1621 actatgtcga acatccaaac gaacgtccgc attcagataa tggttttggt aacgctagcg
     1681 accatgttca aagaaacaaa aatggtca
//
LOCUS       DAVDWM010000094         1660 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVDWM010000094 DAVDWM010000000
VERSION     DAVDWM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.094"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            110..964
                     /locus_tag="VUW49_002117"
     CDS             110..964
                     /locus_tag="VUW49_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7981962.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVKEHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTCIRLAR"
     gene            1114..1371
                     /locus_tag="VUW49_002118"
     CDS             1114..1371
                     /locus_tag="VUW49_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET7981963.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT          526 a          270 c          391 g          473 t
ORIGIN      
        1 atattatcct gaaatggtgt tatttttgag ttatctggct tgactttctt gatggaaatt
       61 atataatagt tgcaacaata ataattacaa cgagaaaaga ggaccattta tgacctatga
      121 atacaagagt cacatttatt tggcagagac agttttaaat gtaaaggatt tggccagtca
      181 aacagccttt tatcagcaag ttattggttt agaaatctta tctcaaactg agacagagtc
      241 cattctaggc cttggtggaa aagtcttggt acagctgatt caagcacaag agagcggaga
      301 agtgaaggaa cattatggtc tttaccattt ggccattctc ttgcccacac gcaaggcttt
      361 ggctgatgtc ttgaaacacc tgacagattt acaaattcct cttgtcggcg gtgcagacca
      421 tggttacagt gaagctattt acttggaaga tttggaggga aatggcattg aactctatcg
      481 agataagcca gtttccacat gggatattcg agaagatgga cgcattatcg gagtgacgga
      541 ggctcttgcg gcgcaggaca tttatgagtt gggggaaaga gtagagccct ttatcctggc
      601 agaaggtacg agaatggggc atattcatct ttccgtcaag gatagtcgaa agtccagcca
      661 gttttatcaa aaggtgttag gactagagga taaattcagt gtgcctggcg ctagttggat
      721 tgcggctgga gattaccatc atcatttagc agttaacgaa tggggaggaa aaggtctggc
      781 ttcgcgtaag caaggcttac caggtttagc ctactatgtc atcgaagtcg cacataaaga
      841 agaactgtta acgattgccc agcgagcaca agaagttgac gcaccaatca aatggatgac
      901 atcgagccaa ttggaaatca cagactcaga tggcatcgtg acctgtattc gtttagcaag
      961 gtaaaattag gaatttttaa taagttataa gtattgttag tttgcttttt gaacggtctt
     1021 tggtaacaga actgcgtgag ctggatgggc gagaattttg acaaagaagc tgattagaga
     1081 tataatgaag aaaaatcgag caaggaaaaa gaaatgacaa attcaaagta tattacttgt
     1141 ttgaaacgtt cagagggcca gttgtgtggg attcaaaaga tgattgaagg agatcgtgac
     1201 tgtgctgata ttgtgacgca actgacagca gtgagatcta gtgtagagcg cgtgattgag
     1261 atgataatta ccgaaaatct tactgaatgt atcaatcagc cactagatga ttctgaagct
     1321 caaaaggaac gcctagaaaa ggctatccga tacttgatta aacggaaata aatgaatgtt
     1381 ttgagacgat atgtgcaaag gtctattaac tattagaata gtttaatgtg agtcaaaaga
     1441 agttgtaaaa aatctttaaa attaaaaaaa cgcatagtat caggtgttga atgtactgac
     1501 ccccaaaagt tagatttttt ctgtctaact tttggggtgc agttcataag aaccttggta
     1561 ctatgcgttt tattatgtag agacttatac tcttcgaaaa tctcttcaaa ccacgtcagc
     1621 ttcaccttgc cgtagatatg gttactgact tcgtcagttc
//
LOCUS       DAVDWM010000095         1594 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVDWM010000095 DAVDWM010000000
VERSION     DAVDWM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.095"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            299..>1594
                     /locus_tag="VUW49_002119"
     rRNA            299..>1594
                     /locus_tag="VUW49_002119"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          445 a          325 c          468 g          356 t
ORIGIN      
        1 accgcctttt cacggcggta acacgggttc gaatcccgta cggactatgg tatgttgcgt
       61 caggaccact tgatgaaaaa aagtttaaaa aagcttaaaa atcttcaaaa aagtgttgac
      121 aagtgaaagt ggctgtgata tactaatata gttgtcgctt gagagaagca agtgacaaag
      181 acctttgaaa actgaacaag acgaaccaat gtgcagggcg ctacaacata agttgtagta
      241 ctgaacaatg aaaaaacaat aaatctgtca gtgacagaaa tgagtgagaa ctcaaacttt
      301 taatgagagt ttgatcctgg ctcaggacga acgctggcgg cgtgcctaat acatgcaagt
      361 agaacgctga aggaggagct tgcttctctg gatgagttgc gaacgggtga gtaacgcgta
      421 ggtaacctgc ctggtagcgg gggataacta ttggaaacga tagctaatac cgcataagag
      481 tggatgttgc atgacatttg cttaaaaggt gcacttgcat cactaccaga tggacctgcg
      541 ttgtattagc tagttggtgg ggtaacggct caccaaggcg acgatacata gccgacctga
      601 gagggtgatc ggccacactg ggactgagac acggcccaga ctcctacggg aggcagcagt
      661 agggaatctt cggcaatgga cggaagtctg accgagcaac gccgcgtgag tgaagaaggt
      721 tttcggatcg taaagctctg ttgtaagaga agaacgagtg tgagagtgga aagttcacac
      781 tgtgacggta tcttaccaga aagggacggc taactacgtg ccagcagccg cggtaatacg
      841 taggtcccga gcgttgtccg gatttattgg gcgtaaagcg agcgcaggcg gttagataag
      901 tctgaagtta aaggctgtgg cttaaccata gtaggctttg gaaactgttt aacttgagtg
      961 caagagggga gagtggaatt ccatgtgtag cggtgaaatg cgtagatata tggaggaaca
     1021 ccggtggcga aagcggctct ctggcttgta actgacgctg aggctcgaaa gcgtggggag
     1081 caaacaggat tagataccct ggtagtccac gctgtaaacg atgagtgcta ggtgttagac
     1141 cctttccggg gtttagtgcc gtagctaacg cattaagcac tccgcctggg gagtacgacc
     1201 gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt
     1261 aattcgaagc aacgcgaaga accttaccag gtcttgacat ccctctgacc gctctagaga
     1321 tagagttttc cttcgggaca gaggtgacag gtggtgcatg gttgtcgtca gctcgtgtcg
     1381 tgagatgttg ggttaagtcc cgcaacgagc gcaaccccta ttgttagttg ccatcattta
     1441 gttgggcact ctagcgagac tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa
     1501 tcatcatgcc ccttatgacc tgggctacac acgtgctaca atggctggta caacgagtcg
     1561 caagccggtg acggcaagct aatctcttaa agcc
//
LOCUS       DAVDWM010000096         1433 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVDWM010000096 DAVDWM010000000
VERSION     DAVDWM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.096"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..1261
                     /locus_tag="VUW49_002120"
     rRNA            <1..1261
                     /locus_tag="VUW49_002120"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1338..>1433
                     /gene="rrf"
                     /locus_tag="VUW49_002121"
     rRNA            1338..>1433
                     /gene="rrf"
                     /locus_tag="VUW49_002121"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          385 a          304 c          423 g          321 t
ORIGIN      
        1 cgacacaggt agtcgaggcg agtagcctca ggtgagcgag agaactctcg ttaaggaact
       61 cggcaaaatg accccgtaac ttcgggagaa ggggtgctga ctttaagtca gccgcagtga
      121 ataggcccaa gcaactgttt atcaaaaaca cagctctctg ctaaatcgta agatgatgta
      181 tagggggtga cgcctgcccg gtgctggaag gttaagagga gtgcttagcg taagcgaagg
      241 tatgaattga agccccagta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
      301 tccttgtcgg gtaagttccg acccgcacga aaggcgtaat gatttgggca ctgtctcaac
      361 gagagactcg gtgaaatttt agtacctgtg aagatgcagg ttacccgcga caggacggaa
      421 agaccccatg gagctttact gcagtttgat attgagtgtc tgtaccacat gtacaggata
      481 ggtaggagtc taagagatcg ggacgccagt ttcgaaggag acgctgttgg gatactaccc
      541 ttgtgttatg gccactctaa cccagatagg tgatccctat cggagacagt gtctgacggg
      601 cagtttgact ggggcggtcg cctcctaaaa ggtaacggag gcgcccaaag gttccctcag
      661 aatggttgga aatcattcgc agagtgtaaa ggtataaggg agcttgactg cgagagctac
      721 aactcgagca gggacgaaag tcgggcttag tgatccggtg gttccgtatg gaagggccat
      781 cgctcaacgg ataaaagcta ccctggggat aacaggctta tctcccccaa gagttcacat
      841 cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg tagtcggtcc
      901 caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga acgtcgtgag
      961 acagttcggt ccctatccgt cgcgggcgta ggaaatttga gaggatctgc tcctagtacg
     1021 agaggaccag agtggactta ccgctggtgt accagttgtc ttgccaaagg catcgctggg
     1081 tagctatgta gggaagggat aaacgctgaa agcatctaag tgtgaaaccc acctcaagat
     1141 gagatttccc atgattatat atcagtaaga gccctgagag atgatcaggt agataggtta
     1201 gaagtggaag tgtggcgaca catgtagcgg actaatacta atagctcgag gacttatcca
     1261 aagtaactga gaatatgaaa gcgaacggtt ttcttaagtt gaatagatat tcaattttga
     1321 gtaggtatta ctcagagtta agtgacgata gcctaggaga tacacctgta cccatgccga
     1381 acacagaagt taagccctag aacgccggaa gtagttgggg gttgccccct gtg
//
LOCUS       DAVDWM010000097         1214 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVDWM010000097 DAVDWM010000000
VERSION     DAVDWM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.097"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(1..404)
                     /locus_tag="VUW49_002122"
                     /pseudo
     CDS             complement(1..404)
                     /locus_tag="VUW49_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(650..961)
                     /locus_tag="VUW49_002123"
     CDS             complement(650..961)
                     /locus_tag="VUW49_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981964.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(976..>1214)
                     /locus_tag="VUW49_002124"
     CDS             complement(976..>1214)
                     /locus_tag="VUW49_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET7981965.1"
                     /translation="INEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTI
                     KVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          367 a          251 c          207 g          389 t
ORIGIN      
        1 cgagcaccaa acaactgctc attttcagat ggtgtaccat agtaaaatcc tgtagccagt
       61 tcacgttggg caaccttcca catctcgtcc accaagtctt gtttgatagc ttcaaacttt
      121 tcaggacttt caagataggc atccacagcc gccttgtagc agttggttac tgttgataca
      181 tagtgaatag actccatacg tccttcgatt tttagactgt ccacaccatt ttcaatcata
      241 tctgagatat ggtcaatcat agacatatca acggctgaca ttgaaaattc ttctggaatc
      301 tcaccctgca aactcttacg ttctttccca aatggcatat cgtaaaggtc gtacttccaa
      361 cggcatgact gagaacatcc accacggttg gcatcacgca tactctagac ggattgtttt
      421 gacaattcta accttttaga ctttgcataa taactgttaa acagttgaaa attaacccgg
      481 ttttaacaag tagtcaaaat accagataaa ggaaaaaagt ctttaaaatc ttaaaaacgc
      541 atagtatcag gtattgaaca atcttgatat tatgcgtttt attgtgggaa gatttacttc
      601 attttctctt ggaattgagt tttctcctac ttcaaaacta gatgatttgc tatttaaaat
      661 agcgttcctt atgattttgg caacctggat taaagcgaga aaaacaaaag gggcaactta
      721 agctttcttt atattgctta tatgttagta gttttagaca aacaccacat aaaataggct
      781 tatcctgtat caaagataag ggatacggct caaaagggtg attttctaat gaatcatgac
      841 accgataaca agcataatac tttttacaat catagcattg taaagcaata atatccagct
      901 tgctatggta atgaatgcat cttgtttgct tatccactaa gaaaccctgt gcctctatca
      961 tttccttttc ctttcttaag ctcgaactgt gacgctggtt ccatcttcct tataaagatt
     1021 gatgagtcct tcttttaatg cacgaaccat gtctcctgat tgaacgggtt gaggcacctt
     1081 aatagtcaat agttccattg gatttggagc gcggtcgatt ttattgcctt tggcatcatg
     1141 caaatcttca atataggttt caaaatgacg gaaacctgga ccataaaact caacttggtc
     1201 cccttcgtta atga
//
LOCUS       DAVDWM010000098         1004 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVDWM010000098 DAVDWM010000000
VERSION     DAVDWM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.098"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            273..1004
                     /locus_tag="VUW49_002125"
                     /pseudo
     CDS             273..1004
                     /locus_tag="VUW49_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          325 a          171 c          203 g          305 t
ORIGIN      
        1 aacgccttca aatcgttcta tagtaaaatg aaataagaac aggacaaatc gatcaggaca
       61 gtcaaattga tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatcta
      121 ttatatttcg tctgatgggc aaatcttata aagagattat agaactttta tagtagtttg
      181 aaataagatg tgaacaactc tatcaggaaa gtcaaattaa tttatagaaa tattttagca
      241 gccaaggtgt actgttatag attcaataca ctatagactg taatcaaaca acgatttggc
      301 gaaatgtaaa aaaatatgag gagttcggac tcgactctct ccttcaagaa acacgtggtg
      361 gtcgtaacca tgcatatatg acggttgagg aagagaaagc ctttcttgcc cgtcatttga
      421 aggctgcaga ggcaggagaa tttgttacaa ttgatgcctt atttcaggct tataaaaagg
      481 agttaggtcg ttcctacaca cgtgatgcct tctatcaact gttgaagcgc catggctggc
      541 gaaatattat gccacgtcca gaacatccta agaaagcaga cgctcaaacc attgtcgcgt
      601 ctaaaaataa aatctcaatt caagaagaca agaaagcgtt ttaaatatag tagacgtttt
      661 cgtaaggttt gcttgatgta ccaagatgag gctggtttcg gtagaatcag taaactggga
      721 tcttgttggt ctccaatagg agtaggtcta catgtccata gtcactatat acgagaattt
      781 cgctattgtt atggagctgt tgatgcctat acaggcgaat catttttctt aatagctggt
      841 ggatgtaata ctgagtggat gaacgccttt ttagaagagc tttcacaagc ttatccagat
      901 gattatcttt tactcgttat ggacaatgct gtatggcata aatcaagtac cttaaagatt
      961 ccgactaata ttggctttgc atttattcct ccatacacac caga
//
LOCUS       DAVDWM010000099          951 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVDWM010000099 DAVDWM010000000
VERSION     DAVDWM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.099"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..>951
                     /locus_tag="VUW49_002126"
     CDS             <1..>951
                     /locus_tag="VUW49_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7981966.1"
                     /translation="DVETKQISINKKEAKIVRYIFERYLEGIGGKVIARELDELGYKS
                     PRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSNFGEEDKYYIKDNHEP
                     IISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSMLECGFCGSILSRRSW
                     HCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAYRQVYHSNENLMTDLL
                     ETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVSDSIYNEKYNEISSEK
                     EFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFD"
BASE COUNT          384 a          117 c          202 g          248 t
ORIGIN      
        1 gatgtagaaa ccaagcagat ttcaattaat aaaaaagaag ccaaaattgt tcgttatatt
       61 tttgaaaggt atctagaagg tattggagga aaagtaattg caagggaact tgatgaactt
      121 ggttacaaat cgccaagagg tttagaacac tggaatgata caacagttct aggaataata
      181 aagaatgaaa aatataaagg tgatattcta atgggaaaaa cttttacagt tgacccaata
      241 agcaagagac ggttgagtaa ttttggagaa gaagataagt actatatcaa ggataatcat
      301 gaaccaataa tatctaaaga agattttgaa aaagctcagg agattagatt gcgtcgagca
      361 ggaaataaga agacagctgc gaatgtaaat gggaagcgtg aacgctattc aaaaatgtat
      421 gcttttagta gcatgttgga atgtggtttt tgtggttcaa tattatctag aagaagttgg
      481 cactgccgtt cagattatcg caaagttgtg tggcactgtg ttacatcgat aaagaaagga
      541 aagaaatttt gtaagcatag taaaggatta gaagagatcg ccattgaggg ggctttcctg
      601 gaagcttaca gacaagtcta tcattcaaat gaaaatttaa tgacagactt acttgaaacg
      661 atagaatctg aattgaatga caatagcctt aacaaggaac taaaaaggat tacaaacaaa
      721 cttcgaatat tactaaaaaa agaggagaat cttgtaaatc taaggctcga aggaaaagtt
      781 agcgattcca tatacaatga aaagtacaat gaaatttcct cagaaaaaga gtttctagca
      841 gaagagaagg taaatattga aacaacatta aaatcagaaa tcgatgtaaa gaaaagacta
      901 actgagttta agcacttgct atcttcacag aaaatgctta cggagtttga t
//
LOCUS       DAVDWM010000100          946 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVDWM010000100 DAVDWM010000000
VERSION     DAVDWM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.100"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            7..>946
                     /locus_tag="VUW49_002127"
     CDS             7..>946
                     /locus_tag="VUW49_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HET7981967.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEF"
BASE COUNT          340 a          171 c          194 g          241 t
ORIGIN      
        1 acagagatgc aagaacatta taccccaaaa gggaaacatt tgacaataga taaccgtcgc
       61 ttgattgagc ggtggaagaa tgaaaataag tccaatcgtg aaattgcagg cttgttagga
      121 aaggcgcctc aaacgattca taatgaagtc aaaagaggta caactttaca acaagtgaga
      181 aaagggctat acaaaaaggt ctattctgcc gattacgcac aaactgttta ccaattcaat
      241 cgaaaacggt cggtgaaaaa gttaatttta acaaaggaaa tcagagagaa gatcttacac
      301 tatcataagc aaaaattttc gcctgaaatg atggttaaca agaagcaagt gaaagttggt
      361 atttcaacca tctactactg gtttcataat ggtcatttag gattgacgaa ggccgacatg
      421 ctttatccca gaaaaaggaa aggtgtcaag aagcaagcta gtccgaactt taagccggca
      481 ggtaaatcta tcgaagaacg tcctgacgtt attaatcttc gcttggaaaa tggtcattat
      541 gaaattgata ccgtcctact gactaagata aaaaattatt gcctgttagt cttaaccgac
      601 cggcggagca gacaccaaat tataaggtta attccaaata aaactgctga atctgtcaat
      661 caggcgctta cgttactatt aggggagcat cgtattctgt ccattactgc agataatggt
      721 tcggagttca aacgattgtc tgaggtattt cctgaggaac atatctacta cgcacatgct
      781 tactcttcat gggagagagg ttcaaatgaa aatcataatc gattaattcg gagatggtta
      841 cctaaaggaa ccaagaaaac gactccgaaa gaagtagctt ttatcgaaaa ttggattaac
      901 aactacccta aaaaatgctt ggactacaag tcgccaagtg aatttc
//
LOCUS       DAVDWM010000101          929 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVDWM010000101 DAVDWM010000000
VERSION     DAVDWM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.101"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            66..926
                     /locus_tag="VUW49_002128"
                     /pseudo
     CDS             66..926
                     /locus_tag="VUW49_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          354 a          167 c          169 g          239 t
ORIGIN      
        1 agagcggaga atgccatcct aaaaaagtta agagaactcc gattgaagga ggaaaaagag
       61 aaagaagaaa gacagaaatt gttcaagaat taatgactga gttttcgtta gatcttcttc
      121 taaaagctat taaactagct cgttggacct actactatca cttgaaacag ctagacaaac
      181 cagataagga ccaagagctt aaagctgaaa ttcaatccat ttttatcgaa cacaagggaa
      241 attatgctta tcgctgtatt catttagaac taagaaatcg tggttatctg gtaaatcata
      301 aaagagttca aggcttgatg aaagtactca atttacaagc taaaacgcga cagaaacgaa
      361 aatattcttc tcataaagga gacgttggca agaaggcaga gaatctcatt caaggccaat
      421 ttgaaggctc taaaacaatg gaacaatgct acacagatgt gacagaattt gccattccag
      481 taagtactta aaagctttac ttatcaccag ttttagatgg ctttaatagc gaaattatcg
      541 cctataatct ttcaacttca cccaacttag aacaagtaca aacaatgttg gaacaggcat
      601 tcacagagaa gcactacgag aatacgattc tccatagtga ccaaggctgg caataccaac
      661 acgattctta tcatcggttc ctagagagta agggaattca agcatccatg tcacgtaagg
      721 gcaacagcca agacaacggt aggatggaat ctttctttgg cattttaaaa tccgaaatgt
      781 tttatggcta tgagaaaaca tttaaatcac ttaatcaatt ggaacaagcc attatagact
      841 atattgatta ttacaacaat aaacgaatta aggtaaaact aaaaggactt agccctgtgc
      901 aatacagaac taaatccttc ggataaatt
//
LOCUS       DAVDWM010000102          748 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVDWM010000102 DAVDWM010000000
VERSION     DAVDWM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.102"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(33..120)
                     /locus_tag="VUW49_002129"
     tRNA            complement(33..120)
                     /locus_tag="VUW49_002129"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Ser,seq:gct)
     gene            complement(127..200)
                     /locus_tag="VUW49_002130"
     tRNA            complement(127..200)
                     /locus_tag="VUW49_002130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ile,seq:gat)
     gene            complement(236..306)
                     /locus_tag="VUW49_002131"
     tRNA            complement(236..306)
                     /locus_tag="VUW49_002131"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Gly,seq:tcc)
     gene            complement(326..398)
                     /locus_tag="VUW49_002132"
     tRNA            complement(326..398)
                     /locus_tag="VUW49_002132"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(363..365),aa:Phe,seq:gaa)
     gene            complement(402..475)
                     /locus_tag="VUW49_002133"
     tRNA            complement(402..475)
                     /locus_tag="VUW49_002133"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Met,seq:cat)
     gene            complement(489..578)
                     /locus_tag="VUW49_002134"
     tRNA            complement(489..578)
                     /locus_tag="VUW49_002134"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(540..542),aa:Ser,seq:tga)
     gene            complement(589..662)
                     /locus_tag="VUW49_002135"
     tRNA            complement(589..662)
                     /locus_tag="VUW49_002135"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(626..628),aa:Ile,seq:cat)
     gene            complement(676..748)
                     /locus_tag="VUW49_002136"
     tRNA            complement(676..748)
                     /locus_tag="VUW49_002136"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Met,seq:cat)
BASE COUNT          216 a          206 c          168 g          158 t
ORIGIN      
        1 ataaaaaaga gagttacaaa ctctcattaa aacggagaat aagggattcg aacccttgcg
       61 ccagttaccc gacctaacga tttagcaaac cgtcctcttc agcctcttga gtaattctcc
      121 aattaatggg cacgagtgga ctcgaaccac cgacctcacg cttatcaggc gtgcgctcta
      181 accacctgag ctacgcgccc aagttaaaaa acttggtaat ttgaacaaag ttcaaagcgg
      241 gtgacgagaa tcgaactcgc gacaacagct tggaaggctg tagttttacc actaaactac
      301 acccgcataa atactatcaa taaaatggcg cgagacggaa tcgaaccgcc gacacatgga
      361 gcttcaatcc attgctctac caactgagct accgagcctt attgcgggag caggatttga
      421 acctacgacc ttcgggttat gagcccgacg agctaccgag ctgctccatc ccgcgttaat
      481 aatataaaag gaggatgtgg gattcgaacc cacgcacgct tttacacgcc tgacggtttt
      541 caagaccgtt cccttcagcc ggacttgggt aatcctccaa tattcaaatg gaccttgtag
      601 gacttgaacc tacgaccact cggttatgag ccgagagctc taaccagctg agctaaaggt
      661 ccgacaagat cattatagcg gcgaagggga tcgaaccccc gacctcccgg gtatgaaccg
      721 gacgctctag ccagctgagc tacaccgc
//
LOCUS       DAVDWM010000103          697 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVDWM010000103 DAVDWM010000000
VERSION     DAVDWM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.103"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(112..585)
                     /gene="tnpA"
                     /locus_tag="VUW49_002137"
     CDS             complement(112..585)
                     /gene="tnpA"
                     /locus_tag="VUW49_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HET7981968.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
BASE COUNT          190 a          159 c          125 g          223 t
ORIGIN      
        1 actggtggtt tgttctgcgg ctataagccg cttctaccgg ccagggccaa aggcccaccg
       61 aaatagcttc ctcgcgcacc actttcccga gcaggtgcta aagcacctta gttacttcct
      121 cttatttatt tcgccagtaa acggatctac tgactcgaat aacgtgagct ggtctgctac
      181 tctgtcttct tgtaattgat tctgaatata ttcagctatc actttctgat tacggcctac
      241 cgtatctaca taatagcctc tacaccaaaa cttgcgattg ccatatttgt attttaaatt
      301 cgcatgctta tcaaaaatca tcaaactgct cttgcccttt aaatagccca taaaggacga
      361 aacactaagt ttcggaggaa tactgataag catgtgaata tggtctgaac aagcattcgc
      421 ttcatggatt attacaccct tacgctcaca taagtcacgt atgattcttc cgatactagc
      481 tttgtatctg ccataaatga tttgacgacg atatttgggt gcaaaaacaa tatgatattt
      541 acaattccat gtggtatgtg ataaactttg attatcctct ctcatgaggt acctcctgta
      601 tgatatgttg tagtggcgga gaaaccactt ctatcttatc attttaggag gttctttttg
      661 ttaccacgct aaaagctcta tggaaccact agcatag
//
LOCUS       DAVDWM010000104          692 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVDWM010000104 DAVDWM010000000
VERSION     DAVDWM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.104"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VUW49_002138"
     tRNA            complement(74..147)
                     /locus_tag="VUW49_002138"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VUW49_002139"
     tRNA            complement(157..242)
                     /locus_tag="VUW49_002139"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VUW49_002140"
     tRNA            complement(250..321)
                     /locus_tag="VUW49_002140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VUW49_002141"
     tRNA            complement(344..416)
                     /locus_tag="VUW49_002141"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VUW49_002142"
     tRNA            complement(435..516)
                     /locus_tag="VUW49_002142"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VUW49_002143"
     tRNA            complement(522..594)
                     /locus_tag="VUW49_002143"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VUW49_002144"
     tRNA            complement(619..691)
                     /locus_tag="VUW49_002144"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          205 c          149 g          159 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DAVDWM010000105          565 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVDWM010000105 DAVDWM010000000
VERSION     DAVDWM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.105"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            151..>565
                     /locus_tag="VUW49_002145"
     CDS             151..>565
                     /locus_tag="VUW49_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET7981969.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRRTVRKCLGKGRFKEVYSADYAQQSYENNRKHSVKRSSVTKELKEKILHYHNQ
                     KFLPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDL"
BASE COUNT          206 a          107 c          114 g          138 t
ORIGIN      
        1 gattctagtt ttgcaggaag tttaatgaag ttagccaccc agccctccta aaaattagat
       61 ggttcaacta gctgaaagct cttgtaggca taagaaaaca cctctgtgtt atacttgttg
      121 ttcaaccaca aacacaagaa aggcacagaa atgcaagaca attatactac aaaaggtaaa
      181 catttgacaa tcgatagccg tcgcttaatc gaaagatgga aaaaagaagg aaaatcaaat
      241 agagaaattg cctctctact tggaaaagct cctcaaacta tccacactga aatcaagcgt
      301 aggacagtcc gaaaatgtct tggaaaaggg cgcttcaaag aggtttattc tgccgactat
      361 gctcaacagt cttatgaaaa taatcgcaag cactcggtca agagatcaag cgtgaccaag
      421 gaactaaagg aaaagattct ccactatcat aaccaaaaat ttttgcctga aatgatggtt
      481 atggctaaag gggttaacgt gggaatttca accatttact attggattca tcatggaaaa
      541 ttggggttaa gcaagcagga tctgc
//
LOCUS       DAVDWM010000106          560 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVDWM010000106 DAVDWM010000000
VERSION     DAVDWM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.106"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            193..533
                     /gene="tnpB"
                     /locus_tag="VUW49_002146"
                     /pseudo
     CDS             193..533
                     /gene="tnpB"
                     /locus_tag="VUW49_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          177 a          107 c          105 g          171 t
ORIGIN      
        1 cagttcattc tacacccact attatgcgtt tttatcattt tgaaatctac tgctaatcaa
       61 tgaagaagca aagatcaaac taggcagtta gttacaagtt acgtaagcgc gtcataacaa
      121 ggtatctatc ctaattcccc aacttccagt atactagata aaaaataatt atcggaggaa
      181 aggtattgta ctatgataat tcaactaagt gatttaggtc aagttcacct tgtttgtagc
      241 aagatagata tgaggcaggg aatagactca ttagcctatg tagttaaaac ccactttgaa
      301 ttggatcctt tctccggtca agtctttctc ttttgtggtg gacgtaaaga ccgctttaaa
      361 gccctttact gggatggtca aggattttgg ctactatata aacgctttga gaacggcaga
      421 ctgacttggc ccaatacaga aaaggatgtc aaagctctca cacctgaaca agtagactgg
      481 cttatgacgg gcttttctat cactccaaaa ataaatccat cagaaagtcg tgatttctat
      541 tgaaatgagg actttctttt
//
LOCUS       DAVDWM010000107          557 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVDWM010000107 DAVDWM010000000
VERSION     DAVDWM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.107"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..478)
                     /locus_tag="VUW49_002147"
     CDS             complement(<1..478)
                     /locus_tag="VUW49_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7981970.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          145 a          132 c           74 g          206 t
ORIGIN      
        1 agtgggtacg gatagtaaac tccttaaaca caatgcgtaa gtctcttaat actctttgac
       61 gccctcgaaa tcgttcattg cttaagacgc gttctagttc ttcttgtttc tctttacttt
      121 gtttgtttct ataatctctt agtgtttcat gaaagagata ataatcatct agccagagac
      181 ccccctcact tatacgatga ctgatctcac ccacttcttc atagggttct ttatcgtatc
      241 tacgcttctg actttcctgc ttacggatat agtctaaaat tcgatttcga aacttggttt
      301 tgaaatattt ccttaaacgt ggaatatcgt ctaccagtcc ttcttctcta ctaatcaatt
      361 catgtaagca gagcatgcct tcttggtccc aatccgacaa ttcccataaa tgaaggtaat
      421 attcatttct acacttatac acagtccctt ggacttcttc atacaattct ttaatcataa
      481 ttttcccctt tctagataca gtctaacaga ttagaaaaca catttgttcc attttctcca
      541 ttctgttccc tttagct
//
LOCUS       DAVDWM010000108          549 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVDWM010000108 DAVDWM010000000
VERSION     DAVDWM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.108"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(231..>549)
                     /locus_tag="VUW49_002148"
     CDS             complement(231..>549)
                     /locus_tag="VUW49_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET7981971.1"
                     /translation="ANGDMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGS
                     MATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGE
                     WVN"
BASE COUNT          152 a          121 c           98 g          178 t
ORIGIN      
        1 atagtttaaa acccgtaact ctcttaaaga gtctcttgag tgccgccatt ctttcctcat
       61 caaaaaaact ccgtagtctc tagcgccata aatccggcta gagattacag agttattttt
      121 taatctaaac ttcctacaag ggtatattat ctactgaaag gatatggtat ttgtagggaa
      181 tactttaaag agttcttaca ggaagtcagt attaactagt tatattaggt ttagtttacc
      241 cattcaccat tggcattgac tctatagcta tctacagttg tgttgactgc aagggcacct
      301 gagccattga catagtacca tttatctgat actttgaacc attggcttgc tttcatagca
      361 cctgatgctt caagatagta ccaggtatct ccatctttca cccaatctgt tgccattgaa
      421 ccgttagcgt ttaggtagta ccatgaacca ttgtattgga gccatcctgt cgccatatca
      481 ccattagcgt ttaggtagta ccatgaacca ttgtattgga gccaacctgt cgccatatca
      541 ccattagcg
//
LOCUS       DAVDWM010000109          521 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVDWM010000109 DAVDWM010000000
VERSION     DAVDWM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.109"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..196
                     /locus_tag="VUW49_002149"
     CDS             <1..196
                     /locus_tag="VUW49_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981972.1"
                     /translation="RFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLK
                     KVLPSCNTFYEAFLSCSCFN"
     gene            114..>521
                     /locus_tag="VUW49_002150"
     CDS             114..>521
                     /locus_tag="VUW49_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7981973.1"
                     /translation="MSKSTSKRYYQVAIPFTRLFYPALVSIDYINCLSETTDKNWHKS
                     DRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKT
                     ITDRVDHSDSEVTTSIYTHVTKNMKDEAINVLDK"
BASE COUNT          182 a          122 c           78 g          139 t
ORIGIN      
        1 aagattccat agaatgggga agttagaact tttatgcgaa gagtttgggc ataaactttt
       61 acctcttcct ccctactcac ctgagtacaa tcctattgag aaaacatggg ctcatatcaa
      121 aaagcacctc aaaaaggtat taccaagttg caataccttt tacgaggctt ttttatcctg
      181 ctcttgtttc aattgactat ataaattgtc taagcgaaac aaccgataag aattggcaca
      241 aaagcgaccg tatttttgtt accaatacag gaaaaccagt tcatagttct atcttgagca
      301 agtctctcca gcgagcaaac gaacgcctta aaaaaccaat tcccaaacat ctgtcccctc
      361 acatcttcag acacaccact attagcatct tatcagaaaa taaaattcct ttaaaaacaa
      421 tcacggacag ggttgatcat tccgactctg aagtcactac ttccatctac acccacgtca
      481 caaagaacat gaaagatgaa gcaatcaatg tactggataa a
//
LOCUS       DAVDWM010000110          519 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVDWM010000110 DAVDWM010000000
VERSION     DAVDWM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.110"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..>519
                     /locus_tag="VUW49_002151"
     CDS             <1..>519
                     /locus_tag="VUW49_002151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981974.1"
                     /translation="NKADGTTTWTETSHTYGKVNVPVVDGYYSDKAEAGSKTVTPEQP
                     EATEKVTYKPLGSLVPKSDDPKFPSTPDVKYPNDPTDPTKPGKPVVPDVPGYKPYLLD
                     PKDPSKPGQLVEPGKELPNLPTNPGDDTPIIYVPIVNDVTKPTKQTVTFEGAGDKTPV
                     ANVQNDYTFTGK"
BASE COUNT          197 a          120 c           97 g          105 t
ORIGIN      
        1 aaaacaaagc tgatggaact acaacatgga ctgaaactag tcatacttat ggtaaggtta
       61 atgttccagt tgtagatgga tactactcag ataaagcaga ggctggatct aagacagtga
      121 ctccagaaca accagaagca acagaaaagg ttacctacaa accacttgga agcttggttc
      181 caaaatctga tgatcctaaa ttcccgtcaa caccagatgt gaaatatcca aatgatccaa
      241 cagacccaac aaaaccaggg aaaccagttg ttcctgacgt accaggctac aaaccatact
      301 tgctagatcc aaaagatcca agcaaaccag gtcaattagt agaaccaggt aaggaacttc
      361 caaatcttcc aacaaatccg ggtgatgata caccaattat ctacgtacca atcgtaaatg
      421 atgtgacaaa accaaccaaa caaactgtaa cctttgaagg agcgggcgat aagactccgg
      481 ttgccaacgt ccaaaatgac tacacattca caggtaagg
//
LOCUS       DAVDWM010000111          484 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVDWM010000111 DAVDWM010000000
VERSION     DAVDWM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.111"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            145..>484
                     /locus_tag="VUW49_002152"
     CDS             145..>484
                     /locus_tag="VUW49_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981975.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLS"
BASE COUNT          142 a          103 c           83 g          156 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gttgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tgttggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tcca
//
LOCUS       DAVDWM010000112          458 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVDWM010000112 DAVDWM010000000
VERSION     DAVDWM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.112"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..>458
                     /locus_tag="VUW49_002153"
     CDS             <1..>458
                     /locus_tag="VUW49_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981976.1"
                     /translation="VDIDSTHFTTYGKQEGVAYNAHYRAHGYHPLYAFEGKTGYCFNA
                     QLRPGNRYCSEEADSFITPVLERFNQLLFRMDSGFATPKLYDLIEKTGQYYLIKLKKN
                     TVLSRLGDLSLPCPQDEDLTILPHSAYSETLYQAGSWSHKRRVCQFSERK"
BASE COUNT          123 a          114 c           87 g          134 t
ORIGIN      
        1 ttgtagatat cgattctacc catttcacaa cttatggcaa gcaagaaggt gttgcttata
       61 acgcccacta tcgtgctcat ggctatcatc ctctttatgc tttcgagggg aagacaggtt
      121 attgtttcaa tgcccagctt cgtcctggta atcgttattg ttctgaagag gcagacagct
      181 ttatcacacc tgttttagaa cggtttaatc aacttctctt tcgaatggat agtggctttg
      241 cgaccccaaa attatacgat ttaattgaaa aaacagggca atactacctc ataaaactca
      301 agaaaaatac tgttctgagc cgtcttggag acctttccct cccttgccca caggatgagg
      361 acttaaccat cttgccccac tccgcctact cagaaactct ctatcaagca ggatcttggt
      421 cgcacaagcg tcgtgtctgc cagttctctg aacgaaaa
//
LOCUS       DAVDWM010000113          444 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVDWM010000113 DAVDWM010000000
VERSION     DAVDWM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.113"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..>444
                     /locus_tag="VUW49_002154"
     CDS             <1..>444
                     /locus_tag="VUW49_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7981977.1"
                     /translation="FGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGN
                     GIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLL
                     ELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVA"
BASE COUNT          157 a           64 c           80 g          143 t
ORIGIN      
        1 gtttggggat cctttaaata ataacaagaa atttgcagta aaaacgggac aacagtgttt
       61 taaattttca agtggaaaat ttcttgataa acatgataga gtttttgaag gttatcctgc
      121 ctatggtgga aatggcattg cttggaaaag tcgaaaatat ttaattgata atcctactat
      181 catcattggt cgtgtaggtg catattgtgg taatgtaaga actacccacg gtaaggtatg
      241 gataagtgac aatgctatat atataaaaga atttaagaat tccgatttca atcttgtatt
      301 tttattagaa ttaatgaaag taattgattt tagtaaattt gctgatttct caggtcaacc
      361 aaagataact caaaaaccat tagaaaatca gaaatatatc ctccccccac tagccctcca
      421 aaatgagttc gcagactttg tagc
//
LOCUS       DAVDWM010000114          388 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVDWM010000114 DAVDWM010000000
VERSION     DAVDWM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.114"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            10..388
                     /locus_tag="VUW49_002155"
                     /pseudo
     CDS             10..388
                     /locus_tag="VUW49_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315045.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          135 a           85 c           64 g          104 t
ORIGIN      
        1 ctgaacttcc tgcatgaaca aggatataaa gtttacacct acaatccttt gctcatcaag
       61 gaatttgcca aatcattatc acttcgcaag actaaaacgg ataagaaaga cgctcatggc
      121 atcgctctta aacttctttc tgaccccaat cgtgaacaat ttcaacacga taatcggcaa
      181 gtagacctaa aaatactggc tagacatatt catcgtctca agaaaaacag tctgattgga
      241 aagtacaata cactcgttgt cttgatatca tctttcctga gttggataaa atcgttggaa
      301 agcattcaga atatacctac caactcttga cacgctaccc taatcctcag aaaaggattg
      361 aggcaggatt tgataagctg atagaaat
//
LOCUS       DAVDWM010000115          368 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVDWM010000115 DAVDWM010000000
VERSION     DAVDWM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.115"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..286)
                     /gene="tnpB"
                     /locus_tag="VUW49_002156"
     CDS             complement(<1..286)
                     /gene="tnpB"
                     /locus_tag="VUW49_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET7981978.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVD"
BASE COUNT          115 a           84 c           67 g          102 t
ORIGIN      
        1 agtctacttg ttctggtgtg agagctttga catccttttc tgtacttagc caaatcaatc
       61 tgccgttctc aaagcgttta tatagtagcc aaaatccttg accatcccag taaaggactt
      121 taaagcggtc tttacgtcca ccacaaaaga gaaagacttg accggagaaa ggatccaatt
      181 caaagtgggt tttaaccaga taagccagtg aatcgattcc ttgtctcata tcagttttcc
      241 cacacacgag atagacctcc cctaggctag atagatgaat tgtcatagag caacacctta
      301 tccaaaagct gttctaccat ttcgagattg agagattgaa aaaaacttac ttcaagcttg
      361 ccaatacg
//
LOCUS       DAVDWM010000116          341 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVDWM010000116 DAVDWM010000000
VERSION     DAVDWM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.116"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            246..>341
                     /locus_tag="VUW49_002157"
     CDS             246..>341
                     /locus_tag="VUW49_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7981979.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNRE"
BASE COUNT          125 a           63 c           57 g           96 t
ORIGIN      
        1 tgaaactaga atagtacacc tctgcttcta aaacattgtt agaaatagat tttactgtcc
       61 tgatcgattt gttctgttct tgtttcattt tactatatgt tcgtaaatga aagttctata
      121 atgaaattag ccacccagcc ctcctaaaaa ttagatggtt caactagctg atagctcttg
      181 taggcataag aaaacacctc tgtgctatac ttgttgttca ccacaaacac aagaaaggca
      241 cagagatgca agacaattat actacaaaag ccaaacattt gacaatcgat agccgtcgct
      301 taatcgaaag atggaaaaaa gaagggaaat caaatagaga a
//
LOCUS       DAVDWM010000117          328 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVDWM010000117 DAVDWM010000000
VERSION     DAVDWM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.117"
                     /strain="416"
                     /db_xref="taxon:1313"
BASE COUNT          103 a           61 c           56 g          108 t
ORIGIN      
        1 ttggggtcaa ggattttggc tactatataa acgctttgag aacggaaaac tgacttggct
       61 aagtacagaa aaggatgtca aagctctcgc acctgaacaa gtagactagc ttatgaaggg
      121 cttttctatc actccaaaaa tatagtagat tgaaactaga atagtacacc tctgcttcta
      181 aaacattgtt agaaatcaat ttgactgtcc tgatcgattt gtcctgttct tatttcattt
      241 tactataaat ccatcagaaa gtcgtgattt ctattgaaat gaggactttc tttttacact
      301 catctgcttt caaaaagcat tctagtcc
//
LOCUS       DAVDWM010000118          304 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVDWM010000118 DAVDWM010000000
VERSION     DAVDWM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.118"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..134
                     /locus_tag="VUW49_002158"
     CDS             <1..134
                     /locus_tag="VUW49_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HET7981980.1"
                     /translation="LKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          100 a           55 c           69 g           80 t
ORIGIN      
        1 tcctcaaatt gtctagtctc tatgcctatt ccgaattgtt ttcagcactt tattctagga
       61 ttagaaaagt caacctgaat cttcctgttc cttatgaacc acctagaaga aaagcgtcgt
      121 taatgatgca ttaaagaaca gtcgagatga aaaaatcgtg tgacgcacca agggaggagt
      181 ctgccctttt gaggaaatct agcgaggaaa aacgatactg gaacagcaga aagtaaaact
      241 gacctcatga ggaggaagaa agtggctcat gaggtcaggg gttttgtaag ttacatctaa
      301 ttga
//
LOCUS       DAVDWM010000119          297 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVDWM010000119 DAVDWM010000000
VERSION     DAVDWM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.119"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            116..>297
                     /locus_tag="VUW49_002159"
     CDS             116..>297
                     /locus_tag="VUW49_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7981981.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIED"
BASE COUNT          110 a           40 c           54 g           93 t
ORIGIN      
        1 aaaaaatcaa acctatcagg ataatacctg gaacaaaaaa gagtgattgc tttttcatat
       61 aatacctctt ctagctgatt ttaataacag cttattttaa ggctttttca ctataatgtc
      121 aagtaaggtt tctggctttc aagataaaaa tgggaaagtc ttgaaaatta tgatagaatg
      181 gtggaaggaa aaattcagga gagtagtagt gactcaaaat gttgaaagtc ttctcgtatc
      241 cattgtaatc agtgcataca atgaagaaaa atatctgcct ggtctaattg aagactt
//
LOCUS       DAVDWM010000120          289 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVDWM010000120 DAVDWM010000000
VERSION     DAVDWM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.120"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            <1..>289
                     /locus_tag="VUW49_002160"
     CDS             <1..>289
                     /locus_tag="VUW49_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7981982.1"
                     /translation="LDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCPDCGSLMKKYD
                     FQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQIPRI"
BASE COUNT           97 a           66 c           47 g           79 t
ORIGIN      
        1 tgctcgatat taaagaccca aacatcaaga ttctagatat catcaatatg gatacccaca
       61 aagaaattat cgctaagctg gattatgagg ctccatcttg ccctgattgt ggaagtctaa
      121 tgaagaaata tgactttcaa aaaccgtcta agatccctta cctcgaaaca actggtatgc
      181 cttctagaat tctccttaga aaacgccgtt tcaagtgcta tcactgttca aaaatgatgg
      241 ttgctgagac tcccctggta aagaaaaatc accaaatccc tcgtatcat
//
LOCUS       DAVDWM010000121          285 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 416
            SAMEA2446330-rid19726763.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVDWM010000121 DAVDWM010000000
VERSION     DAVDWM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446330
            Sequence Read Archive: ERR540667
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929657.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 10:22:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,162
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,989
            CDSs (with protein)               :: 1,989
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 57 of 126
            Pseudo Genes (incomplete)         :: 58 of 126
            Pseudo Genes (internal stop)      :: 42 of 126
            Pseudo Genes (multiple problems)  :: 27 of 126
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446330-rid19726763.denovo.121"
                     /strain="416"
                     /db_xref="taxon:1313"
     gene            complement(<1..101)
                     /locus_tag="VUW49_002161"
     CDS             complement(<1..101)
                     /locus_tag="VUW49_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET7981983.1"
                     /translation="MAYSIDFRKKVLSYCERIGSITEASHVFQISRNT"
     gene            complement(209..282)
                     /locus_tag="VUW49_002162"
     tRNA            complement(209..282)
                     /locus_tag="VUW49_002162"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Asn,seq:gtt)
BASE COUNT           98 a           55 c           44 g           88 t
ORIGIN      
        1 gtattacgtg agatttggaa aacgtgtgat gcttctgtta tactacctat tcgctcacaa
       61 taagagagaa cttttttacg aaaatctatt gaatatgcca taagaagatt ataccacatt
      121 gtgtactatt tttggttcat ttcactataa cacaaaatag attattatta cataacaaaa
      181 aagaggtcta aacctcttaa ctcaattact ccgccagtag gactcgaacc tacgacatca
      241 tgattaacag tcatgcgcta ctaccaactg agctatggcg gatta
//
